LOCUS       DAZOYF010000001        88634 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZOYF010000001 DAZOYF010000000
VERSION     DAZOYF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 88634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.001"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="ACHUOF_000001"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="ACHUOF_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR0609367.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="ACHUOF_000002"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="ACHUOF_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR0609368.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="ACHUOF_000003"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="ACHUOF_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR0609369.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="ACHUOF_000004"
                     /gene_synonym="trmU"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="ACHUOF_000004"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR0609370.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="ACHUOF_000005"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="ACHUOF_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR0609371.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="ACHUOF_000006"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="ACHUOF_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR0609372.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="ACHUOF_000007"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="ACHUOF_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR0609373.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="ACHUOF_000008"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="ACHUOF_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR0609374.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="ACHUOF_000009"
     CDS             complement(8942..9223)
                     /locus_tag="ACHUOF_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609375.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="ACHUOF_000010"
     CDS             complement(9237..10898)
                     /locus_tag="ACHUOF_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR0609376.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="ACHUOF_000011"
     CDS             complement(10882..11208)
                     /locus_tag="ACHUOF_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR0609377.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="ACHUOF_000012"
     CDS             complement(11264..11446)
                     /locus_tag="ACHUOF_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609378.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="ACHUOF_000013"
     CDS             complement(11430..11870)
                     /locus_tag="ACHUOF_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0609379.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="ACHUOF_000014"
     CDS             complement(11870..12166)
                     /locus_tag="ACHUOF_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFR0609380.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="ACHUOF_000015"
     CDS             complement(12163..12501)
                     /locus_tag="ACHUOF_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFR0609381.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="ACHUOF_000016"
     CDS             complement(12498..13709)
                     /locus_tag="ACHUOF_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR0609382.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="ACHUOF_000017"
     CDS             complement(13711..14283)
                     /locus_tag="ACHUOF_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFR0609383.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="ACHUOF_000018"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="ACHUOF_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15781..16005)
                     /locus_tag="ACHUOF_000019"
     CDS             complement(15781..16005)
                     /locus_tag="ACHUOF_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609384.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16046..16159)
                     /locus_tag="ACHUOF_000020"
     CDS             complement(16046..16159)
                     /locus_tag="ACHUOF_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR0609385.1"
                     /translation="MSRVGISEPHHAAGAIRLFLRHLKRYGALLAFRHGPA"
     gene            complement(16234..16308)
                     /locus_tag="ACHUOF_000021"
                     /pseudo
     CDS             complement(16234..16308)
                     /locus_tag="ACHUOF_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16318..16623)
                     /locus_tag="ACHUOF_000022"
     CDS             complement(16318..16623)
                     /locus_tag="ACHUOF_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609386.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16620..16898)
                     /locus_tag="ACHUOF_000023"
     CDS             complement(16620..16898)
                     /locus_tag="ACHUOF_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609387.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17178..18926)
                     /locus_tag="ACHUOF_000024"
     CDS             complement(17178..18926)
                     /locus_tag="ACHUOF_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFR0609388.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18923..19222)
                     /locus_tag="ACHUOF_000025"
     CDS             complement(18923..19222)
                     /locus_tag="ACHUOF_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR0609389.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19228..19461)
                     /locus_tag="ACHUOF_000026"
     CDS             complement(19228..19461)
                     /locus_tag="ACHUOF_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609390.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19463..19683)
                     /locus_tag="ACHUOF_000027"
                     /pseudo
     CDS             complement(19463..19683)
                     /locus_tag="ACHUOF_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19676..19897)
                     /locus_tag="ACHUOF_000028"
     CDS             complement(19676..19897)
                     /locus_tag="ACHUOF_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609391.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19887..20087)
                     /locus_tag="ACHUOF_000029"
     CDS             complement(19887..20087)
                     /locus_tag="ACHUOF_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR0609392.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20217..20396
                     /locus_tag="ACHUOF_000030"
     CDS             20217..20396
                     /locus_tag="ACHUOF_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609393.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20521..20850
                     /locus_tag="ACHUOF_000031"
     CDS             20521..20850
                     /locus_tag="ACHUOF_000031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609394.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20900..21088)
                     /locus_tag="ACHUOF_000032"
     CDS             complement(20900..21088)
                     /locus_tag="ACHUOF_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0609395.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21463..22692)
                     /locus_tag="ACHUOF_000033"
     CDS             complement(21463..22692)
                     /locus_tag="ACHUOF_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0609396.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGEKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22941..24062
                     /gene="roxA"
                     /locus_tag="ACHUOF_000034"
                     /gene_synonym="ycfD"
     CDS             22941..24062
                     /gene="roxA"
                     /locus_tag="ACHUOF_000034"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR0609397.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24111..25337)
                     /gene="pepT"
                     /locus_tag="ACHUOF_000035"
     CDS             complement(24111..25337)
                     /gene="pepT"
                     /locus_tag="ACHUOF_000035"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR0609398.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25587..26723
                     /gene="potA"
                     /locus_tag="ACHUOF_000036"
     CDS             25587..26723
                     /gene="potA"
                     /locus_tag="ACHUOF_000036"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR0609399.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26707..27564
                     /gene="potB"
                     /locus_tag="ACHUOF_000037"
     CDS             26707..27564
                     /gene="potB"
                     /locus_tag="ACHUOF_000037"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR0609400.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27561..28355
                     /gene="potC"
                     /locus_tag="ACHUOF_000038"
     CDS             27561..28355
                     /gene="potC"
                     /locus_tag="ACHUOF_000038"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR0609401.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28352..29398
                     /gene="potD"
                     /locus_tag="ACHUOF_000039"
     CDS             28352..29398
                     /gene="potD"
                     /locus_tag="ACHUOF_000039"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR0609402.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29554..30375)
                     /gene="cobB"
                     /locus_tag="ACHUOF_000040"
     CDS             complement(29554..30375)
                     /gene="cobB"
                     /locus_tag="ACHUOF_000040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR0609403.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30391..31302)
                     /gene="nagK"
                     /locus_tag="ACHUOF_000041"
     CDS             complement(30391..31302)
                     /gene="nagK"
                     /locus_tag="ACHUOF_000041"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR0609404.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31331..32575)
                     /gene="lolE"
                     /locus_tag="ACHUOF_000042"
     CDS             complement(31331..32575)
                     /gene="lolE"
                     /locus_tag="ACHUOF_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR0609405.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32575..33276)
                     /gene="lolD"
                     /locus_tag="ACHUOF_000043"
     CDS             complement(32575..33276)
                     /gene="lolD"
                     /locus_tag="ACHUOF_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR0609406.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33269..34468)
                     /gene="lolC"
                     /locus_tag="ACHUOF_000044"
     CDS             complement(33269..34468)
                     /gene="lolC"
                     /locus_tag="ACHUOF_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR0609407.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34730..35803
                     /locus_tag="ACHUOF_000045"
     CDS             34730..35803
                     /locus_tag="ACHUOF_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR0609408.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35931..39377
                     /gene="mfd"
                     /locus_tag="ACHUOF_000046"
     CDS             35931..39377
                     /gene="mfd"
                     /locus_tag="ACHUOF_000046"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR0609409.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39520..40482
                     /gene="ldtC"
                     /locus_tag="ACHUOF_000047"
     CDS             39520..40482
                     /gene="ldtC"
                     /locus_tag="ACHUOF_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFR0609410.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40565..40777)
                     /locus_tag="ACHUOF_000048"
     CDS             complement(40565..40777)
                     /locus_tag="ACHUOF_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFR0609411.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41062..41694
                     /gene="comR"
                     /locus_tag="ACHUOF_000049"
     CDS             41062..41694
                     /gene="comR"
                     /locus_tag="ACHUOF_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR0609412.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41756..42295)
                     /locus_tag="ACHUOF_000050"
     CDS             complement(41756..42295)
                     /locus_tag="ACHUOF_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0609413.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42522..43826)
                     /gene="ndh"
                     /locus_tag="ACHUOF_000051"
     CDS             complement(42522..43826)
                     /gene="ndh"
                     /locus_tag="ACHUOF_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR0609414.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44234..44776)
                     /gene="ycfP"
                     /locus_tag="ACHUOF_000052"
     CDS             complement(44234..44776)
                     /gene="ycfP"
                     /locus_tag="ACHUOF_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR0609415.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44799..45824)
                     /gene="nagZ"
                     /locus_tag="ACHUOF_000053"
     CDS             complement(44799..45824)
                     /gene="nagZ"
                     /locus_tag="ACHUOF_000053"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR0609416.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45835..46659)
                     /gene="thiK"
                     /locus_tag="ACHUOF_000054"
     CDS             complement(45835..46659)
                     /gene="thiK"
                     /locus_tag="ACHUOF_000054"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR0609417.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46640..47281)
                     /gene="lpoB"
                     /locus_tag="ACHUOF_000055"
     CDS             complement(46640..47281)
                     /gene="lpoB"
                     /locus_tag="ACHUOF_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR0609418.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47296..47673)
                     /locus_tag="ACHUOF_000056"
     CDS             complement(47296..47673)
                     /locus_tag="ACHUOF_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR0609419.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47676..48035)
                     /gene="hinT"
                     /locus_tag="ACHUOF_000057"
     CDS             complement(47676..48035)
                     /gene="hinT"
                     /locus_tag="ACHUOF_000057"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR0609420.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48368..50553
                     /gene="fhuE"
                     /locus_tag="ACHUOF_000058"
                     /pseudo
     CDS             48368..50553
                     /gene="fhuE"
                     /locus_tag="ACHUOF_000058"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50613..52046)
                     /gene="ptsG"
                     /locus_tag="ACHUOF_000059"
     CDS             complement(50613..52046)
                     /gene="ptsG"
                     /locus_tag="ACHUOF_000059"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR0609421.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52341..53138)
                     /locus_tag="ACHUOF_000060"
     CDS             complement(52341..53138)
                     /locus_tag="ACHUOF_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0609422.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53149..54153)
                     /gene="holB"
                     /locus_tag="ACHUOF_000061"
     CDS             complement(53149..54153)
                     /gene="holB"
                     /locus_tag="ACHUOF_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR0609423.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54150..54791)
                     /gene="tmk"
                     /locus_tag="ACHUOF_000062"
     CDS             complement(54150..54791)
                     /gene="tmk"
                     /locus_tag="ACHUOF_000062"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0609424.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54781..55803)
                     /gene="yceG"
                     /locus_tag="ACHUOF_000063"
     CDS             complement(54781..55803)
                     /gene="yceG"
                     /locus_tag="ACHUOF_000063"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR0609425.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55806..56615)
                     /gene="pabC"
                     /locus_tag="ACHUOF_000064"
     CDS             complement(55806..56615)
                     /gene="pabC"
                     /locus_tag="ACHUOF_000064"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR0609426.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56735..57976)
                     /gene="fabF"
                     /locus_tag="ACHUOF_000065"
     CDS             complement(56735..57976)
                     /gene="fabF"
                     /locus_tag="ACHUOF_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR0609427.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58063..58299)
                     /gene="acpP"
                     /locus_tag="ACHUOF_000066"
     CDS             complement(58063..58299)
                     /gene="acpP"
                     /locus_tag="ACHUOF_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR0609428.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58510..59244)
                     /gene="fabG"
                     /locus_tag="ACHUOF_000067"
     CDS             complement(58510..59244)
                     /gene="fabG"
                     /locus_tag="ACHUOF_000067"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR0609429.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59257..60186)
                     /gene="fabD"
                     /locus_tag="ACHUOF_000068"
     CDS             complement(59257..60186)
                     /gene="fabD"
                     /locus_tag="ACHUOF_000068"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR0609430.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60202..61155)
                     /gene="fabH"
                     /locus_tag="ACHUOF_000069"
     CDS             complement(60202..61155)
                     /gene="fabH"
                     /locus_tag="ACHUOF_000069"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR0609431.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61223..62293)
                     /gene="plsX"
                     /locus_tag="ACHUOF_000070"
     CDS             complement(61223..62293)
                     /gene="plsX"
                     /locus_tag="ACHUOF_000070"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR0609432.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62374..62547)
                     /gene="rpmF"
                     /locus_tag="ACHUOF_000071"
     CDS             complement(62374..62547)
                     /gene="rpmF"
                     /locus_tag="ACHUOF_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR0609433.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62599..63120)
                     /gene="yceD"
                     /locus_tag="ACHUOF_000072"
     CDS             complement(62599..63120)
                     /gene="yceD"
                     /locus_tag="ACHUOF_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR0609434.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63319..63903
                     /gene="yceF"
                     /locus_tag="ACHUOF_000073"
     CDS             63319..63903
                     /gene="yceF"
                     /locus_tag="ACHUOF_000073"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR0609435.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64015..64974)
                     /gene="rluC"
                     /locus_tag="ACHUOF_000074"
     CDS             complement(64015..64974)
                     /gene="rluC"
                     /locus_tag="ACHUOF_000074"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR0609436.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65547..68738
                     /gene="rne"
                     /locus_tag="ACHUOF_000075"
     CDS             65547..68738
                     /gene="rne"
                     /locus_tag="ACHUOF_000075"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR0609437.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68935..69877)
                     /gene="flgL"
                     /locus_tag="ACHUOF_000076"
                     /pseudo
     CDS             complement(68935..69877)
                     /gene="flgL"
                     /locus_tag="ACHUOF_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69889..71531)
                     /gene="flgK"
                     /locus_tag="ACHUOF_000077"
                     /pseudo
     CDS             complement(69889..71531)
                     /gene="flgK"
                     /locus_tag="ACHUOF_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71597..72538)
                     /gene="flgJ"
                     /locus_tag="ACHUOF_000078"
     CDS             complement(71597..72538)
                     /gene="flgJ"
                     /locus_tag="ACHUOF_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR0609438.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72538..73635)
                     /gene="flgI"
                     /locus_tag="ACHUOF_000079"
     CDS             complement(72538..73635)
                     /gene="flgI"
                     /locus_tag="ACHUOF_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR0609439.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73647..74345)
                     /gene="flgH"
                     /locus_tag="ACHUOF_000080"
     CDS             complement(73647..74345)
                     /gene="flgH"
                     /locus_tag="ACHUOF_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR0609440.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74398..75180)
                     /gene="flgG"
                     /locus_tag="ACHUOF_000081"
     CDS             complement(74398..75180)
                     /gene="flgG"
                     /locus_tag="ACHUOF_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFR0609441.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75318..75905)
                     /gene="flgF"
                     /locus_tag="ACHUOF_000082"
                     /pseudo
     CDS             complement(75318..75905)
                     /gene="flgF"
                     /locus_tag="ACHUOF_000082"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(76007..76069)
                     /locus_tag="ACHUOF_000083"
                     /pseudo
     CDS             complement(76007..76069)
                     /locus_tag="ACHUOF_000083"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(76089..77228)
                     /locus_tag="ACHUOF_000084"
     CDS             complement(76089..77228)
                     /locus_tag="ACHUOF_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR0609442.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(77253..77948)
                     /gene="flgD"
                     /locus_tag="ACHUOF_000085"
     CDS             complement(77253..77948)
                     /gene="flgD"
                     /locus_tag="ACHUOF_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR0609443.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77960..78363)
                     /gene="flgC"
                     /locus_tag="ACHUOF_000086"
                     /pseudo
     CDS             complement(77960..78363)
                     /gene="flgC"
                     /locus_tag="ACHUOF_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78367..78783)
                     /gene="flgB"
                     /locus_tag="ACHUOF_000087"
     CDS             complement(78367..78783)
                     /gene="flgB"
                     /locus_tag="ACHUOF_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR0609444.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78938..79597
                     /gene="flgA"
                     /locus_tag="ACHUOF_000088"
     CDS             78938..79597
                     /gene="flgA"
                     /locus_tag="ACHUOF_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR0609445.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79673..79966
                     /gene="flgM"
                     /locus_tag="ACHUOF_000089"
     CDS             79673..79966
                     /gene="flgM"
                     /locus_tag="ACHUOF_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR0609446.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79971..80387
                     /gene="flgN"
                     /locus_tag="ACHUOF_000090"
     CDS             79971..80387
                     /gene="flgN"
                     /locus_tag="ACHUOF_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR0609447.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80427..81962)
                     /gene="murJ"
                     /locus_tag="ACHUOF_000091"
     CDS             complement(80427..81962)
                     /gene="murJ"
                     /locus_tag="ACHUOF_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR0609448.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82129..82995)
                     /locus_tag="ACHUOF_000092"
                     /pseudo
     CDS             complement(82129..82995)
                     /locus_tag="ACHUOF_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(82997..83644)
                     /locus_tag="ACHUOF_000093"
     CDS             complement(82997..83644)
                     /locus_tag="ACHUOF_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HFR0609449.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83655..84239)
                     /gene="rimJ"
                     /locus_tag="ACHUOF_000094"
     CDS             complement(83655..84239)
                     /gene="rimJ"
                     /locus_tag="ACHUOF_000094"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0609450.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84475..85683
                     /gene="mdtH"
                     /locus_tag="ACHUOF_000095"
     CDS             84475..85683
                     /gene="mdtH"
                     /locus_tag="ACHUOF_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR0609451.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85747..86394
                     /gene="grxB"
                     /locus_tag="ACHUOF_000096"
     CDS             85747..86394
                     /gene="grxB"
                     /locus_tag="ACHUOF_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR0609452.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86528..87088
                     /locus_tag="ACHUOF_000097"
     CDS             86528..87088
                     /locus_tag="ACHUOF_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0609453.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87194..88240
                     /gene="pyrC"
                     /locus_tag="ACHUOF_000098"
     CDS             87194..88240
                     /gene="pyrC"
                     /locus_tag="ACHUOF_000098"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR0609454.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88314..88559
                     /gene="dinI"
                     /locus_tag="ACHUOF_000099"
     CDS             88314..88559
                     /gene="dinI"
                     /locus_tag="ACHUOF_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR0609455.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        21200 a        23672 c        21931 g        21831 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgtatgtgct gtatgggtgc
    15781 tcagtctctg atccctttca gaaattctat caatgcatct gtctgttcag gatttactgc
    15841 cagcatttca ccggatagcg cgcaacgaac aaaaccatga tgatcctttt caatcagcgc
    15901 ccccgtttcc agaaatgcgc ggtaatcagt gatattcatc gtctccatgt tgtcagcatg
    15961 gtatttatca cgctgtttaa gtatctcatc aaatttcatc ggcattgcgt tttcctctgt
    16021 tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg acgaaaagcc agtagtgctc
    16081 cataccgttt caaatgacgc aaaaaaagcc ggattgctcc ggctgcgtgg tgtggttctg
    16141 atattcctac tctgctaatt tgctcagccc catgtctgcg taagtgcgat ttactgcatt
    16201 tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg cccttgctgc tctgttttac
    16261 cagcgggtcg cccaccagct gcccgtaagc tgcaatttgt gctgtcatat tgccacctca
    16321 gtgaagcgat ccggcattgt tctttttcat ctcttccatc attttttcag tgagcattac
    16381 gcactgaaac cccatgtttt tgatgtctac gggtttcatg cctttgtcag tggatatcac
    16441 aggcagaata tcctgataca catcgcggtc agggtaacta agcacacaaa gctcccatga
    16501 tgccacgggt gaggatcctt cgtctgtttc aacccaccag cctggtgtag ctgcgcttat
    16561 ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt tcaccaaatt ttggttcgct
    16621 catgattcca cctctccggt tttaacgttg attgttgtta cctgttccgc ttcggcaatc
    16681 tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg tcatgaatgc gcttatcagt
    16741 tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt ctatcgttct gattgccacc
    16801 tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg gttctgttct gtctctgcgt
    16861 tttttgaatg atttattaaa tttccctgaa gtgtgcatat ttatttttac cccctcgttt
    16921 aaaaagtttt gagttgtgcc tccccttgtc taccttatct accttagtgg ccctcatgcc
    16981 agtaatggcg ctgctttcag cggggtagag tgcttttatc cactatctac cccgtgtcta
    17041 cctccctgtc tgattcaggt aaaatcaggt agagagggta gatagtgggt agacagtaaa
    17101 aaaaggctat ctacctaact taatgcactg aattaaatgt atttttcttt actcaggtag
    17161 acagcaatta caaaaaatta taaaaacgcg tcgcactcgt ctgttgttat tgcgttagtc
    17221 tgcgttactc ccttaacttt ccgcgtaata tattcatgtc cgtaaacttt cgcggctggc
    17281 ttcatagcct tgccaaagtc atttacgttt agcggtttgc tcctgcctgc gtacgccata
    17341 aacgccagat agacgcggta aaggctgttt ctggtcgtgt acttcactga atcgccaccg
    17401 ccgcccatca tcaggccgcg tgcttcctcc agaaaattca ggaactggca aaactcaata
    17461 accggatccg tctgttgctt tattgccaga gcttcatcac cgtcacgctg ttccagtagt
    17521 aaagcccgtg ctttctcaga gtcggtaaag ttcgccagca atcggcggat aatgacaggg
    17581 atttcagccg caatcttttc cggtagctcc ctgtcttttt cggcctcact aacgatattg
    17641 tcgaaacgga aaatcacgcg acgacgtgcc acacctccgg cccgttcggt gaatatcatc
    17701 gggttattgt tggtcgccag caccaccgcc ctgattacag ccgtgaaacg cttttcgtat
    17761 ttcgggttaa tttccacggg gtcaccgccc gtgattttct tgatgcccgt tccttcgcct
    17821 gtatatttcg gctggtcagc caggacgata agacgactcc cgacaacctg cgcacgtcca
    17881 ccagcatcat caagcgatgt catttcagcg cttaccgtgt tctgtttccc tgccagaagg
    17941 ctggctatgt gtgtgaatgt acttttaccg ctcccgccgt ctccggtggc ctcaataaac
    18001 atctgccagt cgtaccggtt cgccataatc atgtacagcg cggcacatat acgcatcatc
    18061 ttgcgctggt cttttccggc tgcgtgctca agccatttat gaaagtttag cgcgttatcg
    18121 cggatgttct cccctggtgc tggtggcgtg tactcaatgc cgttgtgcgt ggtgatccag
    18181 ttctccggcg tgtgcgggga aaattccccc gttttcaggt caagcgcacc attggcgaac
    18241 ggcagcaaat cgccggacgg ctcgcccatt ggttcggcaa taacttttaa cgcttccacg
    18301 gcgttattga ttacgcgctt gctgaaagtg gccctgtgct ctgaatagat caccaccatt
    18361 tcgcggctaa gttccattgt gctgaccgga caccataccc cgccgcgcca tacgtgaacg
    18421 atttcacttt cctgatgcac acaaacgcca tcaaagcgct cggcaagcag ctgcgcgcgc
    18481 tcactgtctg ccatttgtgc cagttgcgta tttttctgct ccggcatggt aagccctgcg
    18541 gcaatatttt cacgctcagc attcagatag cgccgccagt tttcacactt ctggacgtgc
    18601 attccttcgg ggtaaaaatt tgcgtcctga atatctgccg ccgccagctt ctgaccaatc
    18661 tttttggtct ccaccaaatc cagttctccg gcctggtaca gccttacgcg tttttttcca
    18721 tccggaacaa ttttcagtgc atcaagttcg gcaagctgat ttggcccaag ccacacaggc
    18781 ggcacattat cgccggatgc ggggccatcc tgctcctgcc attgtttcgc gtgcgaccag
    18841 gcatcactac ccgcaaagat aattacctct gtgtctttgt gttttattcc gcgtggctgt
    18901 ttttttacgt tcggtgccag tttcattttt tacccctgaa tacgttaagc atctttttta
    18961 tttcctgaat attggcgcgt gctttctccc tgctggttgg tttactgcgg ggcgctgcct
    19021 gtaccagcgt gaaatcacgc cggaattgat aaacaggcat cacgcaatca tattcgtaac
    19081 cttcacggcg gtaggtgatg cgccgttctg ccacgccttt aatcgttacc atgccgccgt
    19141 actggtcgcg gtaaatatca ccgcgcgtaa atttagggtg agtgttgcca ctggcagtta
    19201 agccagaata tttaagtttc attattttta ttctccggtg tgttgttctt tatatctgtc
    19261 gtgcaataga tctatttctt gcagttccat tattacaggc tcaagaagcg ttattaatgc
    19321 cgtggcaatt cttgattttt gtttgtcgcg ttcattgtcg ccaagtgttt caagccatat
    19381 gcgcaatatt tccagcatgt tttcactgtg agaaagtgca agaaatgcgc ggtctattgt
    19441 ttcgtggtaa atatcacgca tattaatccc cgtccgtcgt ttttcttaaa ataacctctg
    19501 tcacgaaatc agcataatcg gcggcgatat tcagtacatc aagcccgttg atttatattc
    19561 tctcgtggaa agtaagaaaa aagccgctct aataagttct ggcattgacg aaagcgcatc
    19621 agccgcatca tcaggaacgc cggaaaattc ctgtttcagg gaattaaaac gatcatcacg
    19681 cataaccccc cccattttca caatcggcaa caagaatatt tttagcgtca ttcagtgacc
    19741 gcgttgcgat gtatcggata cattccaggg cgaactgtgt ccggtctttc tgttcttctc
    19801 cctgcgcaag ctctgccgtg cattcaatat caataagcgc gtgcatcagc gtagtgagtg
    19861 cggcggctgc tgcgttcggt gtggttttat tgtgcatagg tttccccctg acgaatacgg
    19921 gcggcgaata cagcaacaca accggacggg caacggctac gcgcttcgcg ttccgtccaa
    19981 gcggttacgt ggatgatttg ggattctccg gcactcagtg ccagaaaacg ccacacaaag
    20041 gccgtttgtg tgtgtacaag gtgtggtata tgatttacgg caaccataac ggctcctagt
    20101 ttacgttgtt ggttagaagc cccgttactg ctcctacagt gcggggcttt gctttttgtg
    20161 ttgattgtgt gtaatgtgtc actacacata aatacattac ctgaggtgta attaacgtgt
    20221 caacacacaa aaacgaaagg cgtggtaatc ctccgttcca gttccgttta gatcctgact
    20281 tacgggaaaa gatggaaaag gcacaacaac aggacggcga tgattctctt gctgcctgga
    20341 taaagcgaat aatccgcaaa gaattacagc aacgcggcat agaacccaaa ggataaaaac
    20401 tatcagcgcc gtggtgtgag gtactacggc gcattgcttt acaggcgcac acaatgacca
    20461 acgaagaatc aaccgaaaaa acatcacctg cacgaaaaag acgacgcaga aagaaagagc
    20521 atgaatcaga acgattcgcg ccgtgttctt ttgcccttga gaagtacctt aaagaatact
    20581 catcaaagga gagaacttcg cagttatggc aacgcactga accagactga ttgcattgcc
    20641 caccagcctg atagcggcta tcatccccgt gcttatgttt gggatcacac acacaaacgg
    20701 cgcagcgggt tatctgttca gaaaggcggc tccatttcgg ggccgctttt tttatgcctg
    20761 aaaaacccca attttgtggt tttccagttt caccagggcg aacgaatccc cgcccacgtt
    20821 ctggcgtata ttcaatcttc atggttatag ctctgtgttc agatgattga tgtgtggcgg
    20881 ctgcgtgccg ccagcgtgat taatgaactg ccttgcagct atccttccag gccagaacct
    20941 cggataaaga ccagccaacg gaacgaccgc caagtttacg acgtgatggg aattgtccgg
    21001 ccttttccag gcggtagcgg catgagcggc taaggcctgt tagcttttcg cattcttttt
    21061 cacgtataaa ccgatcagtg cttaacacta ttgccccctt tcgtttctta aagagttatt
    21121 tcgtgttcta ttgcgttggg atgtgtctga ttgtgtcagg atgattcaga gttggcaaat
    21181 gttgaggccg tatggtttac agaaagagga ataatcagga taaaatcctt taaattcatg
    21241 ttaatacaaa ggcataaaat attgtttcat gccttttttc tcgctattta aagagtgatt
    21301 cgctagtgta taaaaaatca gtaacacatt aaaaatcagc tacttataaa tctgtacact
    21361 ttttcacctc ttgttcgtag ttgttccgca ttgttgctca ttgttgcacg ttgtatctgt
    21421 tcgcatatcc agtatgcgca tactgaaaaa acacgaaaaa aattattttc ttctggctac
    21481 tggtagcgtg gttacgtttt catgtgttcc cgccagtatc cctaaccgct ccgtccacat
    21541 atccagcgca ttgcgtttag catccagata acgggaatga ttataaactc gctgcattcc
    21601 tggcatctgg tggcctgtaa gctgctccac gacgtgagga tccacgccta aatcgttcag
    21661 catcgttgta aaggtgcgcc ggatgtcatg tagtgaccag tgagggtgat ttagcctcct
    21721 gtgcgctaat ctgccgtact gcgacacgct tgtttcctgt ttcacttccc ccagcaataa
    21781 gcccgtgtgc ctgttctgct ccaccagctg cgtgacgaac ggcagtattg cttccggtat
    21841 gggccggaat attgccacct tcgttttgct gtgttctttc ggcacggtcc agagcatttc
    21901 ggtaaaatcc cactcgctga tctccgataa cctcagttct accgtccggc atccgaacac
    21961 aatcaggagg cggattaacg cgatgtagta gggggagaat atttttttgt ccagtgcctg
    22021 caataattcg cccagttctt tggtgcttaa gacacgctcg cttatatccg gtttttcccc
    22081 aacgtccgcc acactcatat catcaagaac gttgctgatt gcatagcgcc gcctccggca
    22141 gaacttaagc gcctgtttgc acgtctgtag caagaatccg gcagtaacag gcgttcgctt
    22201 tgccacctgg tcaaaacagg ccagccagtg ccgtagctcg catttatcca gcggcatagc
    22261 accaatgtgc tgtattacgt gattattaag gcgctttttc agggcggcat aatccacgcg
    22321 gttttccttt gcgtacgact caagccagta ggtgagcgca tcgccaaccg ttaccggctt
    22381 taacgcttcc tgtacggtgt aattaagctc atgacgtgga tttttcccct ctgccagcca
    22441 tgcgcgacac tgggcggctt tttccctggc tgatttcagg ctcagatcag gataatttcc
    22501 cagcttaatg cgttccggtg gtgcccccct tcccgttccg gccctgtaag tgaaatacca
    22561 ggttaaaagg ccactggttg aatgcctgac gctcaggttt ccaccgtcat taagaaaggc
    22621 tgttttttgg gcgggtgtgc cgttgatttt cctcagctgt gtatcgctca gtttgttaag
    22681 tgctctgctc ataattttga ttccggtcat cacaattaac tacaccatta actacaccga
    22741 tcgttgcaca aagggctaca acgtgaaaca agctggaaca aggaaaatca caaatctgtt
    22801 tataatcaaa aacataatga acaatctgaa acattatgaa acggcaaaaa acactaattg
    22861 ataaaatatg tccatacttc acgcattacg ttaagcatcc gttataatcg gttgcagata
    22921 ccagcctgtg gatgcttaac atggaatacc aactcactct taactggccc gattttcttg
    22981 aacgtcactg gcagaaacgc ccggtggtgt taaaacgcgg ctttaataat tttattgacc
    23041 cgatctctcc agacgagttg gcgggtctgg cgatggaaag cgaagttgac agtcgactgg
    23101 tcagtcacca ggatggcaaa tggcaggtca gccacggccc gttcgaaagc tacgatcatc
    23161 tcggtgaaac caactggtca ttactggtac aggcagtaaa ccactggcat gagccgaccg
    23221 ccgcgctgat gcgaccgttc cgtgaactac cggactggcg tattgatgat ctgatgattt
    23281 ctttttctgt acctggcggc ggcgtcggcc cgcatctcga tcagtacgac gtgtttatca
    23341 ttcagggtac cggacgtcgt cgctggcgag tgggcgaaaa gctgcaaatg aaacagcact
    23401 gcccacatcc ggatctgtta caggtcgatc cgttcgaagc catcatcgat gaagagctgg
    23461 agcctggtga tattctttat attccgccag gattcccgca tgaaggctac gcgctggaaa
    23521 atgcgatgaa ctattccgtg ggttttcgcg cgccaaatac gcgggaatta attagcggat
    23581 ttgccgatta tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat ccggatgttc
    23641 cacctcgtgc tcatcctgcg gatgttctgc cgcaagagat ggataaactg cgtgagatga
    23701 tgctcgaatt gatcaaccag ccggaacact ttaagcaatg gtttggcgag tttatatccc
    23761 agtcacgtca tgaactggat atcgcgccgc cggaaccgcc ttatcagcca gatgaaatct
    23821 acgatgcgct gaaacaaggt gatgtgctgg tgcgcctggg tggtctgcgc gtattgcgca
    23881 ttggcgacga cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt ccggcactgg
    23941 atgcactcgc cagcaacatt gcgctgactg cggagaattt tggcgatgcg ctggaagatc
    24001 cgtcattcct cgcgatgctc gcggcgctgg tcaatagcgg gtattggttc ttcgaagggt
    24061 aagtttggat ttaagccgga tgcagcatca catgccgcat cccttttcgc ttacttccgt
    24121 tgcgccgtta actcggcaat acggacgatc acctgcaccg ctttttccat accttccaga
    24181 atcacaaact catgcttacc atgatagttg taaccgccag tgaacaggtt cgggcaaggt
    24241 aatcccataa acgacaactg cgcgccgtcg gtaccgccgc ggatcggttt cagttccggt
    24301 tcaatatcgc agtcacgcat cgcctgctgg gcgatatcga gaatatgcgg atgctcaacc
    24361 actttctcgc gcatattgta gtaactgtct tcaatcacca attcaatgta gcaatcagga
    24421 tgtaaccctt tgcccacttt tttggcgatc tccatcattt tacgtttacg cgcttcaaac
    24481 tgtttacggt cgaaatcacg gatgatgtag tgcatatcgg cccgttcaac ggtgcctttc
    24541 atgctcgcca ggtgatagaa accttcatag ccttctgtca tttccgggct ttcatccgcc
    24601 ggaacttccg catgaatacg tgccgccagc gacagcgcat ttaccatcac tcctttcgcc
    24661 gtgcccggat gaacattgtt accgacaatt tttatattga ccgatgcggc gttgaagttt
    24721 tcaaactcca gttcgcctac gccaccaccg tcaacagtgt aagcccagcg ggcatcgaag
    24781 gcatcaacat caaaatgttt cgcccctttg cccacttctt catccggggt aaaggcgacg
    24841 cgaatatcac catgcggaat gtttttctgt tgcaataccg ccagcgcggt catgatttct
    24901 gcaatacctg ctttgtcatc agcacctaac aaggttttac catcggtggt aatcaacgtc
    24961 tgacccagta gctgatgcag caccgggaac ataaccggtg ataaaacttc atcgccgata
    25021 cccagcgcaa tatcgccacc gcgatagttt tcaacaattt gcggattaac atttttgccg
    25081 ctgcaatccg gtgaggtatc cacatgagaa ataaagccaa tcgccgggat atcgccaggg
    25141 acgttagccg gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac attgataagc
    25201 cccatttctt cgagctgctc tttcagcaga tgcaataact tccattggcc ttccgtgctg
    25261 ggaacctgtc tcacccctgc ttttgattgg gtatccagag acacgtagtt caaaaatcgc
    25321 tcaagtagtt tatccatgta gtcaccctca ctttttgtga caacattatt aagaagcaag
    25381 aaaagacaaa tattgcgtca ggtcactttt acccctgcaa gcgggaatat ttatcagcat
    25441 taccttaatg aatataaagc taagccagta attcacaaca aagattggcg attcaagcgg
    25501 tttgccgtag aattaccgcc ctcattagga gtcaccaagg tggttaacca caaaccccgc
    25561 atcggtaagc catccgttgc gtttacatgg gacagagtaa aaaattgaat aaacaaccga
    25621 gttcgctttc accgctggtg caattggcgg gaattcgcaa atgctttgat ggtaaagagg
    25681 tcattcccca gctggatctg actatcaaca atggcgagtt cctcacgctg cttggccctt
    25741 ctggctgcgg taaaacaacc gttcttcgcc tgattgcagg tctggaaact gttgattccg
    25801 gacgcatcat gctggataac gaggacatca cccacgttcc ggcggaaaac cgctatgtga
    25861 acactgtttt ccaaagctat gcacttttcc cccacatgac cgtgttcgaa aatgtggcct
    25921 ttgggttgcg catgcaaaaa acccccgctg ctgaaattac gccccgcgtg atggaagccc
    25981 tgcggatggt gcagctggaa accttcgctc aacgtaaacc gcatcagctc tctggtggtc
    26041 aacagcaacg cgtcgccatt gctcgagcgg tggttaacaa gcctcgtctg ttgttgctgg
    26101 atgagtcgct ctcagcgctg gattacaaac tgcgtaagca aatgcagaac gagctgaaag
    26161 cgttacagcg taagcttggc attacattcg ttttcgtgac tcacgaccag gaagaagcac
    26221 tcaccatgtc ggacaggatt gtggtgatgc gcgatggtcg cattgagcaa gacggcacgc
    26281 cgcgtgaaat ctacgaagag ccgaaaaacc tgtttgttgc cgggttcatt ggcgaaatta
    26341 atatgtttaa cgccacggtg atcgagcgac tcgacgagca gcgcgtacgc gccaacgttg
    26401 aaggccgcga atgtaatatc tacgttaact tcgccgttga accggggcaa aaactgcatg
    26461 ttctgctgcg cccggaagac ttacgtgttg aagagattaa cgacgacaac cacgctgaag
    26521 ggctgattgg ttacgttcgt gagcgtaact acaaaggcat gacgctggag tcggttgttg
    26581 aactggaaaa tggcaagatg gtgatggtca gcgaattctt caatgaagac gatcctgact
    26641 ttgaccactc tctcgaccaa aaaatggcca ttaattgggt agaaagctgg gaggtcgtac
    26701 tggctgatga agaacacaag taagttccag aatgtagtga ttgtcactat tgtcggttgg
    26761 cttgtgttgt ttgtctttct gcccaacctg atgatcattg gcaccagctt tttgacccgc
    26821 gacgacgcca gtttcgtcaa aatggtcttt acactggata actacacgcg tctgctcgat
    26881 ccgctctatt ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc caccctcgcc
    26941 tgcctggtgc tgggctatcc atttgcctgg tttctggcga agctgccaca caaggtgcgt
    27001 ccgctgctgc tgtttctgct gattgttccg ttctggacca actcattgat tcgtatctac
    27061 gggctgaaaa ttttcctcag caccaaaggc tatctcaacg agtttttgct ctggctgggc
    27121 gttatcgaca caccaatccg tatcatgttc acgcccagtg cggtgattat cggtctggtt
    27181 tacattctgc tgccgtttat ggtgatgccg ctgtactcca gtatcgaaaa actggataag
    27241 ccgttgcttg aggcggcgcg cgatctcggt gccagcaagt tacagacttt tatccgcatc
    27301 attattccac tgacgatgcc ggggattatt gccggatgtc tgctggtgat gctgccagcg
    27361 atgggcctgt tctatgtgtc cgacctgatg ggcggtgcga aaaacctgct gatcggtaac
    27421 gtcatcaagg tacagttcct caatattcgt gactggccgt ttggtgcagc caccagcatt
    27481 acgctgacta tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc tcgtttgctg
    27541 aataagaagg tggaactcga atgatcggtc gactgcttcg cggcggtttt atgaccgcta
    27601 tctacgcgta cctgtatatc ccaatcatta ttttgattgt gaactccttt aacagctcgc
    27661 gctttggcat caactggcag ggttttacca ccaaatggta tagcctgctg atgaacaacg
    27721 acagcctgtt acaggcagcg cagcattcac taacaatggc ggtgttttcg gcgacgtttg
    27781 ctacgcttat cggttcactg acggcagttg cgctgtaccg ttatcgcttt cgcggtaagc
    27841 cgttcgttag cggaatgctg tttgtggtaa tgatgtcacc agatatcgtg atggcaattt
    27901 cgctgctggt gctgtttatg ctgctgggta ttcagcttgg cttctggtcg ctgctgttct
    27961 cgcatatcac cttctgcctg ccatttgtgg tggtgacggt gtattcgcgc ctgaaaggtt
    28021 ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc cagcgaattt accattctgc
    28081 gaaaaatcat tctgccactg gcaatgccag cggtggcggc gggctgggtg ttaagcttta
    28141 ccctgtcgat ggacgatgtg gtagtttctt cattcgtcac cggaccaagt tatgaaattc
    28201 tgccgttaaa aatttattcg atggtcaaag tcggcgtatc gccggaagtt aacgcgctgg
    28261 caaccatatt actggtgctg tcgctggtga tggttattgc cagccagctt attgctcgtg
    28321 ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc acctgctcgc
    28381 ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca acacgctgta
    28441 tttctacaac tggaccgagt acgtgccgcc aggactgctt gaacagttca ccaaagaaac
    28501 cggtattaag gttatctatt cgacttacga gtcgaacgaa accatgtacg ccaagctgaa
    28561 aacgtacaaa gacggtgcct atgacctggt ggttccttca acctattacg tcgataaaat
    28621 gcgtaaagag gggatgatcc agaagatcga caagtcgaag ttaacgaact tcagcaatct
    28681 cgatccagac atgctcaaca agccttttga cccgaataac gactactcca ttccgtatat
    28741 ctggggtgcg acggcgattg gtgttaacgg tgatgcggtg gatccgaaat ctgtcaccag
    28801 ctgggccgat ctgtggaagc cagagtacaa aggcagcctg ctgttgaccg acgatgcccg
    28861 tgaagtgttc cagatggcgc tgcgtaagct gggctactcc ggtaacacca ccgatccgaa
    28921 agagattgaa gctgcatata acgagctgaa aaaactgatg ccaaacgtcg cagcgtttaa
    28981 ctccgataac ccggctaacc cgtacatgga aggcgaagtt aacctcggca tgatctggaa
    29041 cggttctgct ttcgttgcac gccaggcggg tacgcctatt gacgtggtgt ggccgaaaga
    29101 aggcggcata ttctggatgg acagcctggc gatcccggca aatgccaaaa acaaagaagg
    29161 cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg ttgctgaaac
    29221 tatcggttat ccaacgccaa accttgcggc gcgtaagctg ttaagtccag aagtggcaaa
    29281 cgataagacg ctctacccgg atgctgaaac cattaaaaat ggcgaatggc agaatgacgt
    29341 tggcgcagcc agcagcattt atgaagagta ttatcagaag ctgaaagcag gacgttaatt
    29401 cagcaagcgg gcggtggtat taccgcccgt tttttagtta gcaaggatgt ggctaatgag
    29461 tcaggacagt aaagttttat cccgctcttt cgtcggtatt gtggtccgct tgtaggcctg
    29521 ataagcgtag cgcatcaggc aatgcttcct gctttataat ccctccagca acttttcaac
    29581 aaattctggc accacctggc ttgccgggcc gtaatatttc tcggcaaatt cattaccaac
    29641 ctgactcggt tcaagattca gttcaacggt gtgagcacca tgtagtttgg cttcgtgtac
    29701 aaacccagcc gccggataga catgcccgga agtaccaatg gcaataaaga catccgccat
    29761 cgacaacgcc atataaattt catccatgcc gagtggcatt tcgccaaacc aaacgacgtg
    29821 cgggcgcaag ggtgccggga actggcaaca atggcattta tcttctggcg taacgtctcc
    29881 ggtccagtcg agaacctgac cactttgcga acaacgaact ttcagcaatt ccccatgcat
    29941 atgaatgaca ttggtattac ctgcgcgttc atgcaggttg tctatattct gcgttaccag
    30001 caaaaagcga tcgccgagag cgtcttgcag tttagccagc gcaagatgcg cggcgttagg
    30061 ctgaatttct ggctgctgca gctgtcgacg acgggcgtta taaaacgctt gcaccagttc
    30121 aggatcgcga tcgaaacctt ccggcgttgc cacatcttca acccgatgtt cttcccacag
    30181 gccatctgcg gcgcgaaagg tacgaatacc tgattccgca gaaattcctg cccctgtcag
    30241 tacgagtact cttggttttt ccattgcttc cggcaccact ttatctctga aaaaaatacg
    30301 ctgacgcaaa cgctcgcgca ggcgacgttt atttttacga aaacgactta accgatgacc
    30361 ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt agatgtagga aggccgcacc
    30421 acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg cgtggaacac gagctacagg
    30481 taagagatga caaggcaacc tgtccgccag ttgcgttgtg attgccggga aattcgataa
    30541 gccaccacca atgacgacca ggtcagggtc aacaatggtc aggatatttc ccagagaaac
    30601 cgctaataaa tccagataac gctcaacgtg cgcccttgcc tgctcatcgc cttgatcata
    30661 aagcgcaata atttcgggag cctgcaacgg ttgatgataa tagtgttgat acagccacgc
    30721 aaaaccgcga ccagacagat aattttcaat gcagccatgc tgaccacagc cgcagcggcg
    30781 taacgggaaa tccaacccca tcatggttaa cgcatcaacc ggcagacgca tatggccaaa
    30841 ctcgccggta atgtagcttt taccagtaat cggtttgccg ttgaaaacca gcccgccgcc
    30901 aacgccggtg ccgagaatta accccatcac cagtggatat tgcgtaaatt catcatccca
    30961 ggcttctgaa agggcaaaac agttggcatc gttatcaagg cgtacatcgc gatcaagacg
    31021 tgcgctcagg tcggcacgca gcggtttgcc gctggcagcg gggacattag cggcatacag
    31081 cttaccatct tctgtttccg gcattcccgg aataccgatg ccgacagaac ctttacagcc
    31141 aaaacgttgg tcagcttcag ctaccagctc acacactgca tctaaaaatg cgtcatagct
    31201 gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc cgaccgctat caaacacgcc
    31261 aagcgcaatt tttgttccac caatatcaaa cccgtaatac attgccgctc cttgttttaa
    31321 tgtactgcct ttactggccg ctaaggactc gcgcagggtc aatattactg gcgcgccgcg
    31381 ccggatacca acttgccaaa agactcagca acaatgctgt gaccagtacg tagaagacgt
    31441 ccagccagtg caattccgat ggcaggaagt caataaaata aatatcgctg gagaggaact
    31501 gatgaccgat cagcttttca atccactcaa taatcggggt aagttgcagt gaaacaacta
    31561 cgccgataac caccccacac aggctgccga acagccctgc cagcaatcca taccagacaa
    31621 agatggcgcg aattaaacca tctttcgccc ccagcgttct taatactgcg atatcgccac
    31681 tcttgtcttt caccgccatc actaaggtgg agacgatgtt gaaacaggcc acgccaatca
    31741 ccagtaccat cgccagatac ataatggcgc ggatcatctg gatatcgcga tacatatagc
    31801 cgtaagtacc aatccaactt ttaatataaa catagctgtt ggtcacttca cccgcatcgc
    31861 gtaccagctt attggcgttg aaaacatccg tcattttaag ggcaatacct gacacgctgg
    31921 aacccatatc aagatattgt tgggcatccg ccagcgggat catggcaaaa ctgtgatcga
    31981 gttgaccact caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt ggctgcatca
    32041 gtttatgctc gggattcgag ttggggatca tgatcgacac ccaatcgccc tgcttcactt
    32101 tcagcgcatc cgccacgcct ttgccgatga taatttgctg ttcgcccgct ttaaaattgc
    32161 gccacgcatc cccctgaaca aacgagggta atgcgctcag acgctgttcc tgttgtgggt
    32221 taacgccctt cacctggatt gcgcgcagat tcgctccact ttccaccaga ccggtgaaat
    32281 tgatatacgg cgcagcggcg gcaatacccg gcactttctg cacgttatcc agtgcttcct
    32341 gccagttagt ccatggctga tttaccgctt cgatttcgcc atgcggcacc accgccagaa
    32401 tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg atcaataccg
    32461 ccacgccgag ggcaatgcca atggtagaaa tgacagagat tagcgacacc atgccgccgc
    32521 gtcgccgtcc gcgactaaaa cgcaggccaa tcaataacga taaaggcatc gccattactc
    32581 cgcccccatc aggctcagtt ccgccgtcag acgtccatca cgcatttcca gttggcggct
    32641 catacgtttc gccagttgca ggtcgtgagt gaccaccagg aaagcggtgc cctgcaagcg
    32701 attcaactcc ccaagcaact ggaagatgct gtcggcgtta cgcgcatcga ggttaccggt
    32761 aggttcatcc gccagtacca gacgcggatt attgaccagc gcacgggcaa tcgctacacg
    32821 ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc gcacgatgct ccagccccac
    32881 cgcttttaac atctccagtg cacagctgtt gatttcagcg ggctttttct tgccaatcag
    32941 cagtggcata gccacgtttt ccagggcagt aaaatccggc agcaggtggt gaaactgata
    33001 aataaagccc agcttctggt tgcgcagttc tgctttcgcc gctgaagaca gtttgctcat
    33061 cggctgaccg ttaaagatca catcgccgga ggttggcgta tccagcccgc ctaacaggtg
    33121 cagcaaggta cttttaccgg aaccagagct accgacaatt gccatcattt caccttcgcc
    33181 cacgctgaaa ctgacattga gcagcacatc ggtttgcaca ctgccttcct gatagcgttt
    33241 gcacaggttg tcgcattgca acaggatctt attcataacg taaagcctca gcgggttgtg
    33301 tggcggcagc gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc atcgccacca
    33361 gcgcaataac aatgacctgt aaaggttcga tagccaccgg cagcgccgcg ccatcaagca
    33421 ggacgccgat tatcggcatc agattattta actggctggc aagcagtgcg ccaagcgccg
    33481 ctccgaggat tgcaccgata atcccggcgc tagccccttg caccataaag accatcatga
    33541 tttgtcgcgg agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc ttctccatca
    33601 ccatcagccc cagcgaggta ataatgttaa acgccgcaac agcgacaatc aggctcagca
    33661 gtaagcccat catatttttt tccatgcgta cggcctggaa cagctcgcct ttacggtcac
    33721 gccagtcctg ccatttgctg ccttcaggca gtttttgctg acttaaagag tcaactctca
    33781 gcggctcatc cagccacaaa cgccagccgg taatattggc tgccggataa cgcatcagac
    33841 gcgaggcatc ctcaatattc accagcattt catagccatc gacttcactg ttggcggcga
    33901 aagtgccaat cacattgaac aggcgctgac ttggaatacg ccccatcggc gtgaactggc
    33961 tggcagatgg caccatcacg cggatttgat caccgcgatt gacgcccaac tgtgaggcaa
    34021 gttgttcgcc gaggatgaca ttatatttcc ccggctcgag gtcagtttgt ttcacattga
    34081 ccagatacgg cgttagtgga tctttttgcg ccgggtcgat accgagcatc accccgaccg
    34141 ccacgctgcg cgcgctttgc agtaccacat caccggtagt aataggtgcg acgcgattaa
    34201 cgccgtccag tttgactgcc gtttccggga gttgctgcgg gttaagagag ccatgctcag
    34261 aagagagaat tgcctgtggc atcaggccaa ggatgttgtt ttgcagctcg cgctcaaagc
    34321 cgttcatcac tgacaatact gtgaccagcg ccatcacccc gagggtaatg ccgatggtag
    34381 aaagccagga gacgaaacga ccgaagcgat ccactgcacg cccacgcatg taacgcaggc
    34441 caataaatag agcgacaggt tggtacatga aatccgtctg gttgctgtag caaagacacg
    34501 agtatataag cgaaagcaag atgggtaaac gtctgaactg taagtgatcg tcagggctaa
    34561 tctgaaaagc ccccgtaaat caatcggcta aattgccata ttttatcatc aatcttgccg
    34621 cctgatctta atctgaaaaa tcatccaata cagacagata cccattacac ctcgggtcat
    34681 aaccagcagg atcaaccgca tgtaaattaa aaaagagcgt gttgttctga tgaaacaaaa
    34741 agagctatgg attaaccaga tcaaagggtt atgtatttgt ctggtggtga tttatcactc
    34801 ggtcattacc ttttatccgc atatgaccac ttttcagcat ccgttatcgg aagtcctgag
    34861 caaatgctgg atctatttca atctttacct tgcccccttt cgtatgccgg tttttttctt
    34921 tatatccggc tatttgattc gccgctatat cgacagcgta ccgtggggaa attgtctcga
    34981 taaacggatc tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc
    35041 gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc
    35101 cgcttatgcc gattccaccg gtgagttcct gcacgggatg atcaccgcca gcaccagctt
    35161 gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt aaaattttta gccgtctggc
    35221 gctgccacta ttcgccttgt ttgtactgct gagtgtggcg gttaatttcg ttcccacgcc
    35281 gtggtgggga atgaacagtg tgatacgcaa tttgccttat tacagccttg gcgcatggtt
    35341 tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg cgccgccatc tgctgatggc
    35401 ttctttgctt accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc
    35461 gctggtatcg attgtggtga tcatgaagct gttttatcag tacgagcaac gattcggtat
    35521 gcgctccacc agcctgctga atgtgattgg ctccaacacc attgctatct acaccaccca
    35581 tcgcattctg gttgaaatat tcagcttaac tctgcttgcg caaatgaacg cagcacgctg
    35641 gtcgccgcaa gtcgaactga cactcctgct ggtttatccc tttgtcagtt tgttcatctg
    35701 tactgttgcg ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt
    35761 ctccccgcct tcactgcccg ctgccgtcag ttactctcgt taaaccatca ccccagccgg
    35821 ggcgattcaa tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag
    35881 atatgccccc atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac
    35941 aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg
    36001 gcgcagcctg tgcaacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac
    36061 tcattgcacc agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagttcaccg
    36121 atcagatggt gatgaatctg gcggactggg aaactcttcc ctacgacagt ttttcgcctc
    36181 atcaggacat tatctcctcg cgcctttcca ccctttacca gctaccgacg atgcagcgtg
    36241 gcatactgat tgttccggtg aatacgctta tgcagcgcgt ttgcccacac agttttctcc
    36301 acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc
    36361 aactggacag cgccggttat cgccatgttg accaggtgat ggagcacggc gaatacgcca
    36421 cgcgcggcgc gttgctggat ctcttcccga tggggagtga gctgccttat cgtcttgatt
    36481 tctttgatga tgaaatcgac agcctgcggg tgtttgacgt cgacagccag cgcacgctgg
    36541 aggaagtaga agcgatcaat ctgctgcccg cgcacgaatt tccgaccgat aaagcggcaa
    36601 ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata
    36661 tttaccagca agtgagtaaa ggcacattac ctgctgggat cgagtactgg cagccattgt
    36721 tcttcagcga accactgcca ccgctgttca gttatttccc tgccaatacc ttgctcgtga
    36781 atactggcga tctggaaacc agtgccgaac gtttccaggc tgacacgctg gcgcgttttg
    36841 agaatcgcgg cgtcgatccg atgcgcccgc tgttgccacc acaatcgctc tggctgcggg
    36901 tggacgagct cttctcagag ctgaaaaact ggccccgagt gcagctaaaa actgaacatt
    36961 taccgacaaa agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc
    37021 aggcgcaaca aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact ttcgacggtc
    37081 cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcac
    37141 gaattaaaat tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt
    37201 atctgatgat tggcgctgcc gaacatggtt ttgtcgataa ggcgcgtaat ctggcgctga
    37261 tctgcgaaag cgatctgctc ggtgaacgtg ttgcccgccg tcgtcaggat tctcgccgca
    37321 ccatcaaccc cgatacactg atccgtaacc ttgcggagct gcatattggt cagccggtgg
    37381 tccatctgga gcacggcgtc ggtcgctatg ccggaatgac cacgctggaa gctggcggca
    37441 ttactggcga gtacctgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt
    37501 cgtcactgca tctgattagc cgttacgcag gtggcgcgga agaaaacgcc ccgctgcata
    37561 aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg
    37621 tggcggcgga attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta
    37681 aacacgatcg tgagcagtat cagttattct gcgacagctt cccgtttgaa accacgccgg
    37741 atcaggcgca ggccattaat gcggtactta gcgacatgtg tcagccgctg gcaatggatc
    37801 gtctggtgtg cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc
    37861 tggcagtaga taaccacaag caggtagcgg tgttggtgcc taccaccctt cttgcgcagc
    37921 agcattacga caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct
    37981 cccgtttccg cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg gaagggaaaa
    38041 tcgatattct gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag
    38101 gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag
    38161 cgatgcgcgc gaacgtggat attctgacgc ttactgcaac gccgatccca cgtacgctga
    38221 atatggcaat gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc
    38281 tggcagttaa aacctttgtc cgtgagtatg acagcctggt ggtccgggag gcgatcctgc
    38341 gtgaaatttt gcgcggagga caggtttatt atctctacaa tgatgtggaa aacattcaga
    38401 aagccgccga acggctggca gaactggtgc ctgaagcacg gattgccatc ggtcacgggc
    38461 agatgcgcga gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg
    38521 tgctggtttg taccaccatt atcgaaaccg ggatcgacat cccgacagcc aacaccatta
    38581 tcattgaacg tgcggatcac ttcggtctgg cgcagctgca ccagttacgc ggtcgcgtcg
    38641 gacgttcaca tcatcaggca tatgcatggc tgctgacgcc gcatccaaaa gcgatgacta
    38701 ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg
    38761 cgctggcaac gcacgatctg gagattcgcg gcgcgggtga actgcttggc gaagaacaaa
    38821 gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgccgtcg
    38881 atgcactgaa agccggacgc gagccgtcgc tggaagatct caccagccag caaacagaag
    38941 tcgagctgcg gatgccgtcg ctattgccag atgatttcat ccctgacgtg aacacgcgtc
    39001 tgtcgttcta caaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag
    39061 tcgagcttat cgatcgcttc ggcctgctgc cagatccggc gcgtaccctg ctggatattg
    39121 cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag
    39181 gcggggtgat cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc
    39241 tgcaaaaaca gccgcagcat taccgccttg atggtccgac gcgcctgaaa tttattcagg
    39301 atttgagtga gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag
    39361 agaacgcgat cgcttaatgt aaatcggggg cgtttctgcg cccccatctg caacatttac
    39421 aaattctttg cactttcctg cactatccga cactgtcacc atccataatt caggcttatc
    39481 tgtttattac aataacctta tatttattat gtatttttgg tgatgatcaa aacgcgtttt
    39541 tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa
    39601 gccaacacct ggccgctgcc gccagcgagc agtcgtctgg ttggcgaaaa caaatttcat
    39661 gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt
    39721 ctcgctctgt tacaagctaa ccccggcgtt gatccttacg taccgcgcgc gggcagcgtg
    39781 ttaacgatcc cgttgcaaac cctacttcca gacgcgccgc gcgaaggcat tgtgatcaac
    39841 attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca
    39901 ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca
    39961 gacaaacgtg caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca
    40021 cagggaattg agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat
    40081 gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc
    40141 ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca
    40201 ctctttagcc aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc
    40261 tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt
    40321 gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat
    40381 gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg
    40441 gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat
    40501 ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta
    40561 atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa
    40621 tacggaaaga ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc
    40681 ccagatttgt ccccgcgtta gcactgatag taccgacttt ttgttggcct tctggcgttg
    40741 actgaacttc gacagccgca aagttggcaa atgacatgga gcttaaaatc gccgcagcga
    40801 tgaggttttt acgtttttca taatagtggc cttatgcaga tgaatgacgt cgcaagaaat
    40861 tacttaacga tcgataacta aatgatagat gtgatctgga tcacatacaa gatatttttt
    40921 ataacaatca ttaattaatt taaaaatctt taaatttcag tcaaatagaa agcaattatt
    40981 tttatgggat ctggactggt gaacggaggt gattattttt ataataacct ttcattcaca
    41041 gtcaccaggt acaacggcat catggcaact gactcaacac aatgtgtaaa aaaaagccgt
    41101 ggccgcccaa aagtgttcga cagggatgcc gcgcttgata aggccatgaa attgttctgg
    41161 caacacggtt atgaagcgac ttctcttgcg gacctcgtcg aagcaaccgg agccaaagcg
    41221 cccacgctat acgcggaatt taccaacaaa gaggggttat ttcgtgccgt tctcgaccgc
    41281 tatatcgatc gttttgccgc taagcatgaa gcacagctgt tttgtgaaga gaaaagcgtg
    41341 gagtctgcgc tggctgacta ttttgctgcc atcgccaact gctttaccag caaagacacc
    41401 ccggcaggct gcttcatgat caacaactgc accaccctct ccccagattc aggagatata
    41461 gccaatacgt tgaaatcacg ccatgcgatg caagagcgca ctttgcagca gtttttatgt
    41521 caacgacaag cgcgcgggga aatcccgacc cactgtgacg tgacacatct ggcagaattc
    41581 cttaattgta ttattcaggg gatgtcgatc agcgcacgcg aaggtgcatc gctggaaaaa
    41641 ctgatgcaga ttgccggaac gactttgcgt ttatggcccg aactggtgaa ataagtcgca
    41701 aaaaaggccc ctcagcctga gcgaatgagg ggccaaattg cagagtacag ccttgttata
    41761 ctttgttatt caaaatcagt tgcccgttac tatctagcgg gatctgcgta cccggatcgc
    41821 ggtccatgcg gattttgccc tgctgatcgc caatcttata ggtcacatca taaccgagca
    41881 ttttttctga cttgtcatac accgttttac aacgctgttg cgtagtcgtg taagtatcgc
    41941 tttcctggag agagccctgg atctggttac cggcatatcc accacccagc gcccccacaa
    42001 cagtggcgac atctttaccg cgaccaccac caaactgatg ccctatcacg ccaccagcaa
    42061 cagcgccgag caccgacccg gtaatgcgat tttcatcctg caccggtcga cgatgggtca
    42121 ctgtgacgtt gcgacactcc tgacgcggtg ttttaaccgt ttccttgatt ggggttgcag
    42181 aaacaacctg agcgtattgc gggccccgtt caaacacgtt cagactggcc actgccgcta
    42241 cgcccaacgc agctgcgaca ccaatcccga tacccgccaa cattgattta ttcacgggaa
    42301 cacctccttc tttgcagtta tgccaatcat gcaagcgaag gagaaaagca gctatcagac
    42361 agaagatgaa aagcgcggga atgactggaa atacttaagg attcggagga gtctgaagga
    42421 atgcctgatg cgacgcttaa cgcgtcttat caggcctacc gctcgtacca tttgtccagg
    42481 gcggataagg cgtttatacc acatccgcca gtgtacgtcg attaatgcaa cttcaaacgc
    42541 ggacggataa cgcggttaat actccccacc agcatcatta atccggtttt aaagtaacca
    42601 tgcagcgcaa tctgatgcat tcggtatagc gagatatata caaagcgcgc aattcgtcct
    42661 tcaatcatca ttgagccgcg cgtcaggtta cccatcaggc taccaacggt ggagaagttc
    42721 gacagcgata ccagcgaacc atgatcttta tactgataat ttttcagcgg cttaccgttc
    42781 atctgcgcca gaatgttgtt cattgcgcaa gtcgccatct ggtgtgcagc ctgagcacgc
    42841 ggcggaacaa agcccccttc cggacgcggg catgacgcgc agtcgccaat agcgtaaatg
    42901 tctggatcgc gagtggtttg cagcgtcggt tccaccacca gctggttgat acggttagtt
    42961 tcaagaccac cgatatcttt caggaagtct ggcgctttga tcccggctgc ccataccatc
    43021 agatcagcct caatatattc gccatcttta gtgtgcaggc cgccttcatc agcactggtg
    43081 accatggttt gcgtcagcac gcgaacgcca agtttcgtta gctcgttgtg ggccgcagca
    43141 gagatacgtg gcggtaatgc aggcaaaata cgttctcccg cttctaccag cgttacgttc
    43201 agggcttcgt tggtcaggcc tttgtaaccg tagctgtgca gttgcttgat cgcgttgtgc
    43261 aattcagcgg agagttctac acccgtcgcg ccgccgccga caatcgcaat gttcactttg
    43321 ccatttgcgc ccaggttggc agagtatttc aggaacagat tcagcatctc ctggtggaag
    43381 cgacgcgcct ggtgcgggtt atcgaggaaa atgcagttct ctttgacgcc tggcgtattg
    43441 aaatcgttag aggtgctacc cagcgccatg accagggtgt cataggcgat tttacgttcc
    43501 ggaaccaaca gttcaccttt ctcgtcgcgt agttctgcaa tagtgattgt tttcgcttca
    43561 cgatcaatat caatgacgga acccaactgg aactggaaac catgattgcg cgcatgggcc
    43621 agatagctca acgcatcgac gccttcatca agcgagccag tcgccacttc gtgcagcagt
    43681 ggtttccaca ggtggctgtg gttacgatcg accagcgtaa ttttggcttt tttcttacgt
    43741 cccagcttat gccccagctg tgttgccatt tccagcccac cagcaccgcc gccgacaatc
    43801 acaatctttt tcaatggcgt agtcaacgtg ccccccttaa atttattaac caattgttaa
    43861 ttaatggtta ttaacatagc tcttaattaa caacaggtta cagcaatgaa aatgttcttc
    43921 gctattgaga ataacatgaa tggtgcattg gtcataccaa aattgatgtt aatcaagttt
    43981 tgttgcttaa aagataggca actttaccgg gggagatcaa aaaatagagt ttattgttta
    44041 ttatttgagc aaattcggaa gggtaatata gggtgaagcg tatatcgagg gcgtttggtc
    44101 gtcctgataa gatgcgcagc atcgcatcag gcattgatgc tcgactgtcg gatgcggcgt
    44161 ggacgcctta tccgacttac agaattcgga gcgcatcagg taccggcaag gttacctgat
    44221 gcgacgaggg catttacccg agggttttga acgctttaat gcgctgtaaa tgcggggaga
    44281 tattcttgaa tttgtgcgtc tgctcttcgt cccagacaat ctcgtaataa tgatgcaact
    44341 cttcagatgt ccgctggctg ttaagtgctt catcattacg cgacaaaata accagacaac
    44401 gatcgcgatt cttctcgcgg aagttggtca cacacttagt ggcaatatcg gcatactctt
    44461 ccgggcgatc aatcttgcct tccatgttct cataagggaa caaattaggg ttgaagatca
    44521 cctggcggat atcgcaaaga aaaccaatcc gttccgccca gtatccgccc aggccaacgc
    44581 cgcaaattag cggacgctcg tcaacgttca gttgcaacat tttgtccact tctttaagca
    44641 gatgctgcat atcatgtttc ggatgccgcg tactgtagct tatcaagcgt acatccgggt
    44701 caataaactg caattgtaag actttctcgt ggttacccgg actgttagag tcaaaaccgt
    44761 gtaaatagat aatcatcgct tcctcacata agccagggtt agtgacctac tttctcttcc
    44821 tgccagcgtt catgtaactg attcagacgg gtgctgatcg ctttccagcg agccgagtcc
    44881 atcagttcct gtcgcgaaaa tgaaccttta tgatacaaac gtgtaacacg ttctgccttg
    44941 atcggtgaca gattatctaa cacgctgacg gcccctttac gattattgca gaccaggatc
    45001 atatcgcaac ccgcatccag tgatgcctgc ccgcgttcgg cataactgcc cataatcgcg
    45061 gcaccttcca tcgataaatc gtcagagaaa atcacgccgt caaaccccag ttcctgacgc
    45121 aaaacggttt tcagccagta gggagaaccg ctcgccggac gcggatcaac atcactgtag
    45181 atcacatgcg caggcataat ggcgtcgagt ttattttcgc ggattaagga actgaagacc
    45241 gacatatctt tagcgcgaat ctccgcttgt ggacgtggat cgcacggtgt ttctttgtgt
    45301 gagtctgccg ttactgcacc gtgtcctggg aagtgtttcc cggtcgtttt cattccggct
    45361 tcatgcatac catcaataaa ccggctggca attgccaggg ctttttgtgg atcggcatga
    45421 taagaacgct cgccaatcgc cgcgctgata tgcccgacat ccagcactgg cgcaaagctg
    45481 atatcaatat ccatagcgat catttcgctg gccatcagcc aacccgcctc ttgcgccagt
    45541 ttgccgccct cttccattcc caacagcgca gcaaaggatt gtgctgccgg taagcgggta
    45601 aaaccttcgc gaaaacgctg cacgcgtcca ccttcctgat ctaccgccac caccagatga
    45661 ttgcgcgatg ctgcgcggat ctggcgcacc agttcacgta actgggcagg atcatgatag
    45721 ttacgcgtaa agagaatcag ccctcccacc agcggatgcg ccagtatttc acgctcttcc
    45781 gcgtccagtt cgtaaccttc gacatccaac attactggac ccacactgct ctccttattg
    45841 ttttattaat agctgccgcc aggtgtcatc ggccagcctg ataaattgtt gatcgccggt
    45901 ttgtcgccag cggtactcaa accaccctcc tttgagcatc agcagccagg gaaaccatcg
    45961 cctgacctga cgccataatt gcgccggata aatcttcgcg cgagtggcat agtcattgac
    46021 caattgccgg tgctgttcag tattttccac ccacaccgcc gccagttcca gcgcgatatc
    46081 accatctcca gcatactccc agtcgatgag ttttaaccct gatgcgctat gcactaaatt
    46141 tccggcgtgg acatccatat gcaatggact taagcgtaaa ggtcgtggtt cccgcgcttt
    46201 gcgcagacgt tttaacattc gcagccaacc cacagtccgc cgcgccggat cgctttgctg
    46261 ccagtacagt tccagtaagg gcaacagcgt tattcgccag ccaaaacgtg gttgttgatg
    46321 tagataatac agcaagcctg ccagttcgtt ggtatccggc aaatacgttt ttaccgcacc
    46381 gggcagatag tcgactacca tccagtcacg gagatataaa tgcggcttcg gtgcaatgct
    46441 tgcgggtagt tgtgataaag cccgatactg gcgcaagaac gcggactgcg gcgcatcagg
    46501 atcgtgcggc tgacgcacaa caaaacgctg gccctgatgt tcaatgagaa aactaccgcc
    46561 actaagccca ctattaaacg tcgtgacggg atgaaactgc gggaaaaagc gcgacagcaa
    46621 ttcgtcgcgc gtgatgggat tattgctgcg aaacggcacc tttacctgac cagataattt
    46681 cgcccgtctg caccagcatc agctgcattt gtagggtcgg agcgttaacg ttgccagagg
    46741 cgcaggagta cagcacgtaa tgagcgccga cattgcgggc aatgcctatg gctttactac
    46801 gggtgcctaa actgtcctgc ggcgacaaac ctaactgttg cttagccatc gacagttgct
    46861 gggcggaaac cagggtaaat ttcccgttat tagccagcgc atttcgcagc gtttcggtcg
    46921 cttctgcggc attcagcgaa ccgttagtac ggttgttaac gctatcaacc agcaggacgc
    46981 tacccgcagt caccccgtca gccccaagca tcttactgac catcggctgc attgcgccat
    47041 tccagtcata atggcgaata tgcggcgcag gcggtgccgt tcgatcttcg tgctcaattg
    47101 ggcctggctg ctgcgggatc gtcggcaccg agggcactgt ggggacaggc tgttgtggct
    47161 cggctggttg ttccggcgct ggtttcactt cttctaccgg tgcaggttca cgttgcccca
    47221 cacacccggc gagaaacatc gccagcgcgg taatcaaggc gtagcgactc atttttgtca
    47281 tcaagattca cccctttaca aatatagata aagtctgact ttgtgcgccc ccagaaaatt
    47341 ggcgctgccg tacagcgtta ccgccgaatg tgcgggaatg gtaacgctgc gtggcctttc
    47401 cagaggatgc atctccagcc ctctggcgtc ataccagtaa aaacgataat gaacggtaac
    47461 gggttcttgc ctttcgttat aaatcgttga ggatgctgaa ggttgaatat cagacgtcga
    47521 aaggacgggc ttttctgcac tgatgcccgc agccaataaa gatgactcca tcaccagcga
    47581 ttgctcatca ttcaccggaa tttccggatg tgaacgacag cccaccagca gtaacaacac
    47641 cagagacacc agcccaaagc atccttttct catcgttaca gacctttatg cgccagcatt
    47701 ggtcccagcg gacggccacc caacaagtgc atatggatgt ggtaaacctc ttgtccgcca
    47761 tggcggttgg tattcatgat cagacgatag ccatcttcgg caataccttc ttgctcagca
    47821 atttttgccg ctacggtgat catgcgtccc agcgcctgct catgctcagc tgacacgtcg
    47881 ttcacagtcg gaatgagaat attcggaatg atcagaatat gcgttggcgc ttgcggcgaa
    47941 atatcgcgaa acgccgttac cagatcatcc tggtagacga tatcggaggg gatctcacga
    48001 cgaataattt tgctgaatat agtttcttct gccacgacat tttccttttt caaatagcca
    48061 tgcgttgcgc catgctacgc cgcgccacac tgcgagtata gagtatggtc gagttaaacc
    48121 tgctctttca atcttaactc acgatttctt aagcaaaaaa tcgttttgct gccgtatata
    48181 tcgccattat tcccatttct gcctgcgaat gcgctgaaaa gacattctct gtttggttta
    48241 cacgacaaat atgaatgcgt atatttctca tttgcatttg cagcaaaatt attcgcacac
    48301 taaatataac cgctcatcac cctcctccgg catgagcctg acgacaacat aaaccaagag
    48361 atttcaaatg ctttcaacac aatttaacag ggataatcaa tatcaagtca tcaccaaacc
    48421 gtcactactt gccggttgca tagcactggc actattacct tctgccgctt ttgctgcacc
    48481 agccactgaa gaaacggtga ttgttgaggg ttcagccaca gctccagatg atggcgaaaa
    48541 tgattacagc gtaacgtcta cctctgcggg taccaaaatg cagatgactc aacatgatat
    48601 tcctcagtcg gtcactattg ttagccagca gcggatggaa gatcagcagt tacaaacgct
    48661 gggcgaagtg atggaaaaca cgctggggat cagcaaaagt caggcggatt ccgatcgtgc
    48721 tctttattat tcccgcggat tccagatcga taactatatg gttgatggta tccccaccta
    48781 ttttgaatcg cgctggaatc tgggcgacgc actttctgat atggcactgt ttgaacgcgt
    48841 agaagtagtg cgtggcgcga caggactcat gaccgggacg ggtaatccat ctgcggcaat
    48901 taatatggtt cgaaaacacg cgacctgtcg tgaatttaaa ggcgatgtct cggcggaata
    48961 cggtagctgg aacaaagaac ggtatgtggc ggatttacaa agcccactca ccgaagacgg
    49021 taaaatccgc gcgcgaattg tcggcggcta ccagaataac gactcatggc tggaccgcta
    49081 caatagtgaa aagaccttct tctcgggcat tgtcgatgct gatttaggcg atcttacgac
    49141 gctttcagcc ggttacgaat atcagcgcat tgatgttaat agcccaacct ggggcggttt
    49201 accgcgctgg aatactgatg gcagcagcaa cagttacgat cgcgcacgca gtaccgcacc
    49261 tgactgggcg tacaacgata aagagatcaa caaggtcttt atgaccctga agcagcggtt
    49321 tgctgatacc tggcaagcga cactgaatgc cacccactct gaagtcgaat ttgacagcaa
    49381 aatgatgtat gtcgatgcct atgtaaacaa agcggatggt atgctggttg ggccatacag
    49441 taattatgga cctggctttg atcatgtcgg cggcaccggt tggaacagtg gcaaacgtaa
    49501 agttgatgcg ctggatttgt tcgctgacgg tagttatgaa ttgtttggtc gtcagcacaa
    49561 tctaatgttt ggtggcagtt acagcaaaca aaacaatcgt tcagttcatg ggccaacatc
    49621 ttcccggatg aaattggcag tttctacaac tttaatggca atttcccaca agccgactgg
    49681 tcatcacaga gcctggcgca ggacgatacc acacatatga aatcgttata tgctgccact
    49741 cgtgtcaccc ttgccgatcc gctgcatctg atcctcggcg cacgttatac caactggcgg
    49801 gttgatacgc tgacttacag catggagaaa aaccacacca cgccttacgc tggtctggtg
    49861 tttgacatca atgacaactg gtcgacctac gccagctata cctctatttt ccagccgcaa
    49921 aatgatcgtg acagttcagg caaatatctg gctccaatca ccggtaacaa ctacgagctg
    49981 ggtctgaaat cggactggat gaatagccgt ctgaccacca cgttagccat cttccgtatt
    50041 gagcaggata atgtcgctca gtccaccggt acacctatcc ccggcagcaa cggcgaaacc
    50101 gcctataaag cggtggatgg gacagtcagt aaaggggtgg aatttgaact caacggcgca
    50161 attaccgaca actggcagct gacatttggc gcaacgcgct atcttgcaga ggataacgaa
    50221 ggaaacgccg ttaatcctaa tctgccacgc accacggtta aaatgttcac cagctatcgg
    50281 ttgcctgtca tgccagagtt gacagtcggc ggtggtgtta actggcaaaa tcgcgtgtat
    50341 accgacaccg tgacaccgta tggcaccttc cgcgccgagc aaggtagcta cgcgctggtg
    50401 gatctcttca cccgctacca ggtgacgaaa aacttctcgt tacaggggaa cgtcaataac
    50461 ctgttcgaca aaacctacga taccaacatg gaaggttcta tcgtctacgg cgcaccgcgt
    50521 aatttcagca ttaccggcac gtatcaattc tgaataacac ctgtaaaaaa ggcagccatc
    50581 tggctgcctt agtctcccca acgtcttacg gattagtggt tacggatgta ctcatccatc
    50641 tcggttttca ggttatcgga tttagtaccg aaaatagcct gaacaccaga accagcaacc
    50701 actacgcccg ctgcgcccag tttcttcagg ccagcctgat ccactttaga cacatcagca
    50761 acgctgacgc gcagacgggt aatacatgcg tcgaggttag taatgttttc tttaccacca
    50821 aatgcagcaa ccagagccgg tgccatttcg ctggtacctg ttgcttttgc atcttcagtc
    50881 gcgtcttcac gacccggcgt tttcagatcc agtgctttaa tcagcacgcg gaagatggtg
    50941 tagtaaacaa tcgcataacc gataccgacg atcggtaaca gccacagttt gctgctgtta
    51001 ccagacagaa cgatgaagtc gatcagaccg tgcgagaacg acgtaccgtc acgcatcccc
    51061 agaagaatac agattgggaa tgccaggcct gccagaatcg cgtggatgat gtacaggatc
    51121 ggcgcaacga acatgaagaa gaactcgatc ggctcggtga taccggtcag gaacgaggtc
    51181 agcgccgcgg agatcataat accgcccact ttcgcgcggt tttctggttt agcagagtgc
    51241 cagatagcaa ttgcggcagc tggcagaccg tacattttga acaggaagcc accagacagt
    51301 ttacccgcag tcgggtcacc cgccatataa cgcggaatgt cgccgtggaa aacctgacct
    51361 gctgcgttgg tgtattcacc aatctgcatc tggaaaggta cgttccagat gtggtgcaga
    51421 ccaaacggta ccaggcaacg ttcgatgaaa ccgtaaatgc caaacgcaac taccgggttc
    51481 tggtaagcag cccactgaga gaaggtctgg attgcagaac caatcggcgg ccaaatgaag
    51541 gacagcacaa cgccagtaaa gatggcagcc aggccagaaa tgatcggcac aaagcgttta
    51601 ccggcaaaga agccaagata ctcaggcagc ttaatacggt agaaacggtt aaacatgtac
    51661 gctgcaatcg caccggagat aatccctccg agtacgccag tatccgccaa gtgtttagag
    51721 gcgatttctt cagcaggtaa atgcagtacc agtggcgcaa ccacggccat ggttttaacc
    51781 atgatgccat aggcaacaac tgcggccagc gcggatacgc catcgttatt ggtaaagccg
    51841 agggcgacac cgatcgcaaa aatcagtggc atgtttgcaa agacggaacc gcctgcttct
    51901 gccataacat gcgatacaac ggcgggcagc cagctgaaat tcgcggaacc gacgcccagc
    51961 agaatacctg cgataggtag tacggatacc ggcagcatca gcgatttacc gaccttttgc
    52021 aggttagcaa atgcattctt aaacataatt gagagtgctc ctgagtatgg gtgctttttt
    52081 tacgttctca cgcgtggcaa ggggggagag cctcgccgtg tacagggcat ctaagcgccc
    52141 tttatttatt acacagagta aaataattca gtgccaattt gtttgacggc tatcacgttt
    52201 caacttcaga tcattggaga agttgcacat tttgacaaaa catatgtcac aatatttcaa
    52261 acgcggttat tcaccgccca attttcggcg gtgaacttta ctcgttttag ccattaatta
    52321 cgagctttaa aaaaactcat tcaacggatg gattgaaggc gggaagcgtc gatgtgaaac
    52381 agacgggcga agttatcggt ggttacctgc gccagttctt caacggcaac acctttcaac
    52441 acagccatgt attctgcaac gtcacgaacc atcgcaggtt gattctcttt tcctcgatgc
    52501 ggtaccggcg caaggtaagg tgagtcagtt tccaccagta atcgatccag ggggacataa
    52561 cgcgcagcat cgcgcagttg ctccgcatta cggaaggtca caatgccgga aaaggagatg
    52621 taaaatccga gatccagtaa tttacccgcc gtttctctgt cctctgtaaa acagtgtagt
    52681 acgccaccgc aatccgtcac tttttcttcg cgcagaattg ccagcgtatc ggcacgggcg
    52741 tcacgggtat ggacgataac cggcttgttc agttcacgac caatctggat atgatggatg
    52801 aaggactctt gctgacgtac tttagtttcc ggtgtgtaat aataatccag cccggtttca
    52861 cccagcgcta caacaccctc ttctgccgcc agacggcgta aatcttctac atcgtaggga
    52921 tcattctggt tgagtggatg cacgccacaa gaaaatacga cgttgtcacg ttcgcctacc
    52981 agatcccgca tatgtaaata acccggtaat gttgtggcga ctgccagaca aaatttcaca
    53041 tcgcgtgcgg cggctttcgc cagaacgtca tccacgtcct tatgcaaaga ttcataatcc
    53101 agaccatcga gatggcagtg tgagtcgact aaaaacatga tgtctctctt aaagatgagg
    53161 aaccggtaac acaacgcccg gttgcaggta atgctcaata cgcagtaaaa gatcggtgat
    53221 gagaagctcg cggttgatgc ctgtgacaga cattaactgt tcacgaatgt ggcaaacatc
    53281 ccccagtata gcctgcaggc acgagggaga aagatggttt gccagttcgg cgaccaggcc
    53341 cggcacatca acattggtca cctgcgcagc gccatgatgg cgttttagcg catccatcag
    53401 caacgttgcc agccagtgta aacgcgccgg agcttgttca tgattaaggg ccgccagcag
    53461 cgaataccag tcgcccgatg gcacgctata tgccaacgcc tgacacaatg tttcacgagc
    53521 ctgccagtta tctccctgaa acaacgccag tgccgcgcca ggcgaaccgg cgcttaagcg
    53581 caatgcggca agtaatgcat cctgtgacat tgtcacttcg cgtgaaagcc aagtcacggc
    53641 gtactgttct ggaggcggcg caaggtaatg taaccgacaa cgactacgta atgttgccag
    53701 taaacgttca ggctcgcggg tagccaggaa aaaccaggtt tctgctggtg gctcttcaag
    53761 cgttttcagc aatgcgttag ccgcggcgtc ggtcagtaag gcagcatcgg ttacccagac
    53821 aactttcgca ccacctaggc gtgcgtgctc attcagcttt tcggtgacct cacgtaccgc
    53881 atcaatgccc agcgtatttt tccctttttc gggagccagg gtgtagtaat cgggatgcgt
    53941 gccagcctgc atcaactgac atccacgaca gtgaccgcaa cttttgtggc cctgcggttg
    54001 ttggcagagt aaataacggc tcagggcgta gattaaagca tcatcgccca tgcccggtaa
    54061 cgcctgaatg agtagcgcat ggtgacctct tccggcctga tagctggcta ccagtttttc
    54121 gaaatcaggt cgtaaccatg gataccatct catgcgtcca actccttcac ccagtgggtc
    54181 acggtagtgc ggattgcatc catcacggcc tccagcggct gggtggcatc aatggtatga
    54241 atacttttat cttgtgcagc cagttccaga tagcgggcgc gggtgcgatt aaagaaatcg
    54301 aaagactctt gctcaatacg atccagctcg ccgcgcgcac gcgcgcgttt taagccaact
    54361 tccggggtaa catcgagata gagcgttaag tctgggcgaa aatccccgag aacagcatca
    54421 cgcagtgttg ccagcatatg ttggtcaata ccacgtcctc cgccctgata cgcctgagtg
    54481 gaaagatcgt ggcgatcgcc aatcacccag gtgccgttag ccagcgcagg tttgatgacc
    54541 gtttctacca gttgaacgcg cgcggcataa aacatcagaa cttcggcttt atcggtaatg
    54601 acttcatcgc ctaccgattt gatatccagc accaggcttc ttaacttttc ggcaagttgc
    54661 gtaccgccag gttcccgagt gaaaaccatg tcgcggatac ccagttgctc gagcgtctca
    54721 accaccacat tacgcgcggt agttttgcct gcgccttcca gcccctcaat gacgatatac
    54781 ttactgcgca tttttttcct taagcacttt cagataatcc tgcacagact tgttatgact
    54841 ggcaagattg gtattaaacg tgtgaccacc tttaccatcg gccacaaaat agagatacgg
    54901 cgtttttgcc ggatgcgcag cagccttcag cgaatccgcc cccggcgtag ctatcgcacc
    54961 cggcggcaaa ccggtaatgg tataggtgtt atacgctgtc ggcgtttcca ggtctgcacg
    55021 agaaagtttg ccattataac gctctcccat cccgtaaatc acggttgggt cggtctgcag
    55081 gcgcatacca atgcgtaaac ggttgataaa taccgaggca acctgatcgc gttcactggc
    55141 aacggcggtt tctttttcga taattgatgc catcgtcacc agctgatttt tatctttata
    55201 aggcagaccg tccgcacgcc cttcccaggc gctatcgacc gctttcacca ttttcttgtg
    55261 aactcgcttg agtaacgcga catcggtggt attggcggta tacatcaagg tgtctggcca
    55321 gaaccaacct tcaatccact ccgggttttc cagttcaagt gcctgcgcta cggtggcgta
    55381 cttatcgtca ctcagcgtgt gcttgatata cggagcctca cgcaattgct tgaggtagtc
    55441 gctcagacgc atcccttcta ccagtcgcag cgggaactgt gcttctttac cgctttccaa
    55501 caatttcagc atctcgcgca cggtcatctg cggtgtaaag cggtaagtcc cggctttaaa
    55561 gtgagaaaga tccggttcga tacgcagcag ccattgaaaa acccgtgggc gattgatgat
    55621 cttatcagca taaagctgtt caccgagcgc cagacgtccg gtccctggct tcagggtaaa
    55681 tatcgtctct tctttgataa gcaatttgct gtcggcaaga tggcgaacct tccagacgcc
    55741 cacaccagcg gcgataccca gtaccaccag caataacaag ataatcaata acactttttt
    55801 catgactaat tcgggcgctc acaaagtggg gctaaatatt catataacgt tgctgacgaa
    55861 aaggtgacat cgccacaggc acgtacgggc atcactggca ttaacgcatt acaaataacc
    55921 atctcatctg cctgcaacgc ctcctccagc gaggcttgca cttcgacaag ctgataagag
    55981 gattgtgcca gtaaacggat acagaattgt cgcataatgc cgttaacacc tgcctgatcc
    56041 agtcgcggcg tataaactac attgcccttc cgccagaaca aattagccgc acagcattcc
    56101 gtaacccacc cttcgctgtc aaggaccagc gcctcatcag cgtttgtctg ctcaagatga
    56161 gagcgaatca atacttgctc aagccgatta agatgtttaa tacctgcaag atgaggattg
    56221 cgccccagcc gcaccgggct tagcgccaac gtcatccctt cgttacgcaa acggtcgtaa
    56281 tgtgcaggat aagccgtaac ggagagaatc cgcgttgctg gtccgctgtt caatgtgctg
    56341 taccctcgcc cgccactacc gcgactgatc acgactttca gcacaccatt ttgctgttct
    56401 gctgccagcg ttttcatctc ttgttcaagc tgaggccaga agtcacagga aatcatcaac
    56461 cgctgacaag catcctgtag tcgcaggata tgcgccgata acaaactgac tttaccgtcg
    56521 ataactctgg cggtggtaaa acaaccatca ccaaactgcg ttgcccgatc gcttactgcc
    56581 agcgattcct gcttataacc gttaattaag aacataccgg ctccttatca gagaattgtt
    56641 agtgtggcag catgttcact acggaacaag tcggaataaa agctaagaaa aaaggcccgc
    56701 aagcggacct tttatatggg tggaaaatga caacttagat ctttttaaag atcaaagaac
    56761 cattagtgcc accgaagccg aaggagttac acagcgtgta ttccattccg ctgacctgac
    56821 gtgcttcatg cggtacgaaa tccagatcgc aaccttcatc cgggttatcc aggttgatgg
    56881 ttggcggaac agcctgatcg cgcagcgcca ggatggagta gatagattct actgcacccg
    56941 ccgcacctaa caggtgaccg gtcatagatt tcgtggagct taccaacaca cggcttgcag
    57001 cttcaccgaa gatggttttc accgcctgcg cttcagcttt atcgccagcc ggcgtagaag
    57061 taccgtgcgc gttaacgtag ccaatctgac tcgcttcaat gcctgcatca cgcagagcat
    57121 ttgccattgc cagagctgcg cctgcgccat tttctggcgg tgacgtcata tgataagcat
    57181 cgctgctcat accaaagccg acgagttcag cgtaaatttt cgcaccgcgt tttttcgcgt
    57241 gttcgtactc ttcaagtacc agcataccgg caccatcgcc cagtacgaag ccatcacgct
    57301 ctttatccca cgggcggctc gccgcttgcg ggttatcatt gcgggtagat aatgcacgtg
    57361 ccgcgccaaa accaccaacg cccagcggcg tactggcttt ctctgcgcca cctgcaacca
    57421 tcacgtcagc atcgccatac gcgataatac gcgcagcatg gccaatgttg tgcacgccgg
    57481 aagtacaggc ggtcgcgata gagatgcttg ggccacgcag gccatacatg atagtcagat
    57541 gacctgccac catgttcaca atcgttgacg gaacgaagaa tgggctgatc ttacgtggac
    57601 caccgttcat cagagatgtg tggttttctt cgatcagtcc gaggccgcca atcccggagc
    57661 caattgcggc accaatgcgg gttgcgttct cttccgttat ttcaaggcca gaatcctgca
    57721 tggcctgaac gccagcgaca attccatatt gaatgaaggc atccatcttg cgctgttctt
    57781 tgcgcgagat aatgtcctca cagttaaaat cctttactaa gccagcaaat ttcgttgcat
    57841 aggcgctagt atcgaaatgg tcgattaggc tgatgccact ctgaccggca agcagagctt
    57901 tccaggtaga ctctacggta ttgccgacag gagacaacat gcccagtccg gtcacaacta
    57961 cacgacgctt agacacgttt gtcctccaag gagggaaaaa tgattctagt gggacaaaaa
    58021 gataaaactc aggcggtcga acgaccgcct ggagatgttc acttacgcct ggtggccgtt
    58081 gatgtaatca atggcagcct gaacggtggt gattttctca gcttcttcgt ccggaatctc
    58141 agtatcaaac tcttcttcca gagccattac cagctcaacg gtgtcaagag aatccgcgcc
    58201 caggtcttca acgaaagaag cattgttggt aacttcttcc tgcttaacgc ccagctgttc
    58261 gccgataatt ttcttaacgc gttcttcgat agtgctcata ctcttaaatt tcctatcaaa
    58321 actcgctttc gcgatggttt tcgtagtgta taaaatgttg aaaaatttgc aactaaatcc
    58381 cggcaggtct taccacgatt ttacgttatt ttgaggcctt accccccaat aacgcaaatc
    58441 attttgcact aattattact cataaccacg cagactgcgc gcaacatgag cattttgtgc
    58501 aaatcgcggt cagaccatgt acatcccgcc gttcacatgc aaagtttcac ccgtgatgta
    58561 agctgcttcg tcggatgcca ggaatgcaac cgcgttggcg atttcctgtg cgccgccgag
    58621 gcgacccgca ggaacctgcg ccaggatacc cgcacgctgg tcatcgctca gcgcacgtgt
    58681 catgtccgtt tcaataaagc ccggagcaac aacgtttaca gtaataccgc gtgacgcaac
    58741 ttcgcgcgcc agtgatttac tgaagccgat caagcccgct ttcgccgcag cgtagttggc
    58801 ctgaccgcca tttcccatgg taccaaccac agaaccgata gtgataatac gaccatgacg
    58861 ctttttcatc atagcgcgca ttaccgcttt tgacagacgg aaaacagatg aaaggttagt
    58921 ttcgataata tcgttccact cttcatcttt cattcgcatt aacaggttat cacgagtgat
    58981 accggcatta ttgaccagga tatccacttc accaaattct gcgcgaattt tttccagaac
    59041 agattcgata gatgccgggt cggtcacatt caacatcaga cctttgccgt tggcacctaa
    59101 ataatcactg atcgcctgag cgccattttc actggtcgca gtgccaataa ctttcgcgcc
    59161 acgggctgcg agcgtttcag caattgcgcg gccaattccg cggcttgcac cggttaccag
    59221 tgcgattttt ccttcaaaat tcatgatttt cctcttttaa agctcgagcg ccgctgccat
    59281 cgctgaaggt tcgttcagcg ccgaggcggt cagggtgtcg acaatgcgtt tcgtcaggcc
    59341 agtaagcacc ttgcccgggc cgacttcata gagatgttct acgccttgcg ctgccatgta
    59401 ctcaacagac ttcgtccact gaaccgggtt atacaactga cgtaccagtg cgtcacggat
    59461 ggcatcacca ttggtttcgc atttcacatc aacgttattc acaacagaaa ctgttggtgc
    59521 gttaaaggtg attttcgcta attctactgc cagtttgtcg gctgctggtt tcatcagcgc
    59581 acagtgagac ggtacgctca ctggtaacgg cagcgcgcgt tttgcgccag cagctttaca
    59641 ggcagcgcca gcacgctcaa ccgcttcttt atgaccggca ataaccacct gtcccggaga
    59701 gttaaagttt accggagaaa cgacctgacc ttctgcagct tcttcacacg ctttcgcaat
    59761 aggcgcatca tccagaccga tgattgccgc catagcgccc gtgccttccg gtacggcttc
    59821 ttgcatgaac ttaccgcgca tctcaaccag acgcaccgca tcagcgaaat caatcacgcc
    59881 agcgcaaacc agcgcggagt attcccccag gctgtgaccg gccatcattg ccggtgcttt
    59941 accgccctgc tgctgccata cgcgatacag cgcaacagat gcagtcaaca gcgctggctg
    60001 ggtttgccag gttttattca gttcttcagc tgacccctgc tgggtcagcg cccacaggtc
    60061 gtagcccagc gccgcagaag cttcagcaaa cgtttcttcg acaattggat agctcgccgc
    60121 catatcagcc agcattccaa cggtttgaga accctgtcca gggaacacaa atgcaaattg
    60181 cgtcatgttt taatccttat cctagaaacg aaccagcgca gagccccaag tgaatccacc
    60241 gccaaaggct tcaagcagaa ccaactgccc cggcttaatg cgcccgtcgc gtacagcttc
    60301 atccagcgcg cacgggacag aggccgcaga ggtattaccg tggcgatcca gcgtcaccac
    60361 gacattgtcc atcgacatac cgagtttttt cgccgttgca ctgataatac gcaggttagc
    60421 ctgatgcgga accagccagt ccagttgaga acggtcaaga ttattcgccg ccagcgtctc
    60481 atcaacgatg tgcgccagtt ccgttaccgc aaccttgaag acttcgttgc ccgccatcgt
    60541 cagatgaatt gaattctctg gattcacgcg gtcggcgttt ggcagcgtca gcaattcacc
    60601 ataactaccg tcggcatgca gatgggtgga aatgattccc ggctcttcag aggcagccag
    60661 caccgcagcg cccgcgccat cgccaaaaat aataatagtc ccacgatctg ttggatcgca
    60721 ggtgcgcgcc agtacatcgg aaccgacgac cagagcatac ttcaccgccc cagatttcac
    60781 gtattgatcg gctacgctta atgcataggt gaaacctgca caggctgctg caacgtcaaa
    60841 tgccgggcaa cctttaatgt ccaacatgct ttgaatctga caagctgcgc tcgggaaagc
    60901 gtgcgtagca gaagtcgttg ccacaacgat caggccaatc tggtctttct caatgcccgc
    60961 catctcaatt gcgcgtgtcg ccgcttcaaa gcccatggtt gaaacggttt cgtttggcgc
    61021 ggcaatgtgg cgttcgcgga taccggtacg agtgacaatc cactcgtcag aggtgtccac
    61081 cattttttcc aaatcggcgt ttgtccgcac ttgttcgggc agatagctgc cagtaccaat
    61141 aatcttcgta tacatgtacg ctcagtcact tttcggttat ataccgtcac ttgcaaactg
    61201 cgagttcgct ggcagcgtcc tgctaccgca gagttccgct tttgccaccg tccagcagct
    61261 caaaaccagc tgggtataca gattccaggc gagcggcaat tcgctgagga acttgtcgct
    61321 gcaccgcctg cactgcctgt tcaatcgcga ccgcaaaagc tcgctgattg gctgcaccat
    61381 gactttttat caccgtgccg cgcaatccta acagacaggc gccgttatac tggtcggggt
    61441 tgaggtgact gaatcgcctc gtcaggctct tttgtagcca acgctttaat aacagtagcc
    61501 accacgaccg ttttttccct tcaccctgag atttcagcag agaaaggaac atcctgacaa
    61561 caccttccat cgtctttaac gtgacatttc ctgtaaagcc gtcacaaacc agcacatctg
    61621 tcttgccagt taacaactca ttggcttcaa gatagccgat ataattgata gaagggattg
    61681 ttttaagcac cgctgaggca tcccgaatac tgtcaagacc ctttacttct tcttcaccaa
    61741 tattgagcaa cgccacgcga ggattgggaa tttccaccac ctcttcagcc agaactgagc
    61801 ccataatggc aaattgcacc aacattgtgc tgtcacaatc gacgttggcc cctaaatcga
    61861 ggaccaccgt tttgcccttt tgctgatgtg gtaataccgt caccagcgcc ggacgctcaa
    61921 tcccctccag gggcttgagt aataattttg ccagccccat cagtgccccg gtattaccgg
    61981 cactgacaca ggcttgcgct cgaccttctt tcaccagctc cagggccata cgcattgaac
    62041 tcccacggct ggcgcggata gcttgcgaag gccgggcatc actggcgata actgactgcg
    62101 caggaataat ctgcagacgc gaacgttgtt caaagtcagc tttagcaagt aatggcgtga
    62161 tggcgtcggg attgccgact aaaagaagag tgagttgcga attagaattc agtgcctgca
    62221 atgctgcagg cactgtcacg gaagggccaa aatcccctcc catgacatct aacgccaggg
    62281 ttagacgtgt caaggtatcg tcgccgcccg gtttggtctt tccccagttg cctggggaaa
    62341 atcctcacta agcttcatca cgcagacgcg tgattactta gcgatgacct tgcggccgcg
    62401 gtagtaaccg tcggcagtga tgtggtgacg caggtgtttt tcaccagaag ttttgtctac
    62461 agacaggctg gtgactgcgg tcagcgcgtc atgggaacga cgcatgccac gtttggaacg
    62521 ggttggttta ttctgttgta cggccatgga ccttactcct caattacggt ttatccccga
    62581 tccaacgggg agcaccaatt acttacgctt taagctggct aatacggcaa atgggtttgg
    62641 cttttgcact tcttcaggca gttcaccaaa gaccatgtcc gcttcggaca cttcacagtg
    62701 ttcagaatca tgcaccggaa ctaccggcaa ggcgaggatg atttcatctt caaccattgc
    62761 aagcagatcg atttcaccga attcgttaac ctcaatcggt tcatacgctt ccggcagtgc
    62821 ttcagcctgt tcgtctgaac gcacaggact aaaacaatac gttgtgtaga cctgatgagt
    62881 aaacggcttc ccgcaacgct gacactcgag cgttaccgtc accttcgcat cgccgtttaa
    62941 cactgcgaga cgttggttat cgatagcgaa cgacatggag cattccacat cactgtccac
    63001 actgactacg gattcggcga cgcgctcaac ctgatcaggg gtatagatac cctggtaatc
    63061 aaggcgtttt tgagccgtac gaaccggatc gagagtcagg ggtaatttta ccttttgcat
    63121 agggcgcgca tattaacttt gtaacgtcat agagtcaaag aaaaaggcag cctgtggttg
    63181 ccttttgcca ataattcgca cacattgcgg gttactgctt tattttcgtt cagtgctgat
    63241 aacgaacatc aacctgcaaa acgaagcgca tagtttaaaa tggctatcat caatacgcta
    63301 tatctgatgg aaaaaaatat gcctaaactt attttagcct ccacctcgcc atggcgtcgc
    63361 gctcttctgg aaaaactgca aatctctttt gaatgtgcag cacctgaggt cgacgaaacc
    63421 ccacgcagcg acgaatcacc gcgacagttg gtgcttcgac tggcacaaga aaaatcgcaa
    63481 tctctggcgt cacgttatcc ggatcattta attattggtt cagaccaggt atgtgttctt
    63541 gatggcgaaa tcaccggtaa accgttaacg gaagaaaatg cccgtctgca attacgcaaa
    63601 gccagcggca atatcgtcac cttctatact ggactggcgt tgtttaattc ggcgaatggg
    63661 catctacaaa cagaagtgga gccttttgac gtccatttcc gtcacctaag cgaggcggag
    63721 attgataatt acgtgcgtaa ggagcatccc ctgcactgcg cgggtagctt taagagtgaa
    63781 ggatttggca ttacgctgtt tgagcgttta gagggacgtg accctaacac gctggttggt
    63841 ttaccgctaa tcgcgctgtg tcagatgtta cgtcgggaag ggaaaaaccc gctgatggga
    63901 tagttgtttg cgccggatgc ggttacgtcg catccggcaa tcggtgcagg cggcctgatg
    63961 cggcgtaaac gccttatcag gccaaccgcc cattatcagg cttatatgct tgtattagcg
    64021 cgcgttacgc agcttttgca aacaacgctt caaaccttca tccatcggcg cttcgatacg
    64081 catcacctca ccggtccccg gatgggtaaa cttcaacgct gcggcgtgca ggaacagacg
    64141 atttaatccc gtgcctgctt cagtgagctg tctgtcaaat tcacggtcac cgtagcgatc
    64201 gtcaaaggca atcggatgac ccgcatactg tgtatgcaca cggatctgat gagtacgtcc
    64261 ggttaccggg ctacaacgca ccagggtggc aaatgcatag cgttcttcca ctttaaagcg
    64321 tgtttccgac ggtttgcctt cctgactgac acgcacgata cgttcgccgc tttgcagaat
    64381 atttttcaat aacggcgctt gaacgctctt cacatgcgac tgccactgac cgcgcaccag
    64441 cgccaggtaa tctttttgca ttcctttttc acgtaattgc tcatgcagag aacgcaacgc
    64501 cgagcgtttt ttcgctacca gcaacacacc tgaagtgtcc cggtcgagac gatgaaccag
    64561 ttcaaggaac cgcgcttccg ggcgcaacgc ccgcaaacct tcgataacgc cgaagcttaa
    64621 accactgccg ccatgtaccg ccgtaccgga aggtttattc agcaccagga tatgatcatc
    64681 ttcatataag atgacgtccg ccagcgccgc caccttttgc agatgtggcg aaaccgcctc
    64741 ttcttcccgc tcagcaacgc gaaccggtgg gatacgcacc tcatcacccg cttcgagttt
    64801 atattcaggc ttaatacgtt ttttgttcac ccgcacttca cctttacgca aaatacggta
    64861 aatcatactt tttggtacgc ctttcaattg ggtacgcaaa aagttatcga tacgttgccc
    64921 cgcttcgtca gcggtgatag caacaatttt tacggatgga gtctctgttt tcatggtggg
    64981 cgattctaat tagccaacag gattcgcgcc actcattttt ctatgcttat ttttagtttt
    65041 gcaccttatt acttcactac gtgatcactt tattgatggt tattaaacca atcaccagca
    65101 agaagtgaaa aaactgtgag taagcgggtg ataaatggta aaagtcatct tgctataaca
    65161 aggcttgcag tggaataatg aggccgtttc cgtgtccatc cttgttaaaa caagaaattt
    65221 tacggaataa cccattttgc ccgaccgatc atccacgcag caatggcgta agacgtattg
    65281 atctttcagg cagttagcgg gctgcgggtt gcagtcctta ccggtagatg gaaatatttc
    65341 tggagagtaa tacccagtct gtttctttga taattgcgct gtttttccgc atgaaaaacg
    65401 ggcaaccgac actctgcgcc tctttgagct gacgataacc gtgaggttgg cgacgcgact
    65461 agacacgagg ccatcggttc acacccggaa aggcgttact ttgcccgcag cttagtcgtc
    65521 aatgtaagaa taatgagtaa gttacgatga aaagaatgtt aatcaacgca actcagcagg
    65581 aagagttgcg cgttgccctt gtagatgggc agcgtctgta tgacctggat atcgaaagtc
    65641 cagggcacga gcagaaaaag gcaaacatct acaaaggtaa aatcacccgc attgaaccga
    65701 gtctggaagc tgcttttgtt gattacggcg ctgaacgtca cggtttcctc ccactaaaag
    65761 aaattgcccg cgaatatttc cctgctaact acagtgctca tggtcgtccc aacattaaag
    65821 atgtgttgcg tgaaggtcag gaagtcattg ttcagatcga taaagaagag cgcggcaaca
    65881 aaggcgcggc attaaccacc tttatcagtc tggcgggtag ctatctggtt ctgatgccaa
    65941 ataacccgcg cgcaggtggc atttctcgcc gtatcgaagg cgatgaccgt accgaattaa
    66001 aagaagcact ggcaagcctt gaactgccgg aaggcatggg gcttatcgtg cgcaccgctg
    66061 gcgtaggcaa atctgctgag gcgctgcaat gggatttaag cttccgcctg aaacactggg
    66121 aagccatcaa aaaagccgct gaaagccgcc cggctccgtt cctgattcat caggagagca
    66181 acgtaatcgt tcgcgccttc cgcgattatt tacgtcagga tatcggcgaa atccttatcg
    66241 ataacccgaa agtcctcgaa ctggcacgtc agcatatcgc tgcattaggt cgcccggatt
    66301 tcagcagcaa aatcaaactg tacaccggcg agatcccgct gttcagccac taccagatcg
    66361 agtcgcagat cgagtccgcc ttccagcgtg aagtccgtct gccatccggt ggttccattg
    66421 ttatcgacag caccgaagcg ttaacagcca tcgacatcaa ctccgcacgc gcgacccgcg
    66481 gcggcgatat cgaagaaact gcgtttaaca ccaacctcga agccgccgat gagattgctc
    66541 gtcagctgcg cctgcgtgac ctcggcggcc tgattgttat cgacttcatc gacatgacgc
    66601 cagtacgcca ccagcgtgcg gtagaaaacc gtctgcgtga agcggtgcgt caggaccgtg
    66661 cgcgtattca aatcagccat atttctcgct ttggcctgct ggaaatgtcc cgccagcgcc
    66721 tgagcccatc actgggtgaa tccagccatc acgtctgccc gcgctgctcc ggtaccggta
    66781 ccgtgcgtga caacgaatcg ctgtcgctct ctattctgcg tttgatcgaa gaagaagcgc
    66841 tgaaagagaa cacccaggaa gttcacgcca ttgttcctgt gccaatcgct tcttatctgc
    66901 tgaatgaaaa acgttctgcg gtgaatgcga ttgaaacgcg tcaggacggt gttcgctgcg
    66961 tgattgtgcc aaacgatcag atggaaaccc cgcactacca cgtgttgcgc gtgcgcaaag
    67021 gggaagaaac gccgacctta agctacatgc tgccgaagct gcatgaagaa gcgatggcgc
    67081 tgccgtctga agaagagttc gctgaacgta agcgtcctga acaacctgca ctggcaacct
    67141 ttgccatgcc ggatgtaccg ccagcgccaa ccccagctga acctgccgcg actgtcgtag
    67201 ccccagcacc gaaagctgca acggcaacac cagcagctcc tgcacaacct gggctgttaa
    67261 gccgcttctt cggcgcactg aaagcgctgt tcagcggtgg tgaagaagcc aaaccgaccg
    67321 agcaaccaac accgaaagca gaagcgaaac cggaacgtca acaggatcgt cgcaaacctc
    67381 gtcagagcaa ccgccgtgac cgtaatgagc gccgcgacac ccgtagtgaa cgtactgaag
    67441 gcagcgataa tcgcgaagaa aaccgtcgta atcgtcgcca ggcacagcag caaactgccg
    67501 agacgcgtga gagccgtcag caggctgagg taacggaaaa agcacgtacc accgacgagc
    67561 agcaagtgcc gcgtcgtgaa cgtagccgcc gccgtaacga tgataaacgt caggcgcaac
    67621 aagaagcgaa ggcgctgaat gttgaagagc aatctgttca ggaaaccgaa caggaagaac
    67681 gtgtacgtcc ggttcagccg cgtcgtaaac agcgtcagct caatcagaaa gtgcgttacg
    67741 agcaaagcgt agccgaagaa gcggtagtcg caccggtggt tgaagaaact gccgctgccg
    67801 aaccaattgt tcaggaagct ccagctccac gcacagaact ggtgaaagtc ccgctgccag
    67861 tcgtagcgca aactgcacca gaacagcaag aagagaacaa tgctgataac cgtgacaacg
    67921 gtggcatgcc gcgtcgttct cgccgctcgc ctcgtcacct gcgcgtgagt ggtcagcgtc
    67981 gtcgtcgcta tcgtgacgag cgttatccaa cccagtcgcc aatgccgttg accgtagcgt
    68041 gcgcgtctcc ggaactggcc tctggcaaag tctggatccg ctatccaatt gtacgtccgc
    68101 aagatgtaca agttgaagag cagcgcgaac aggaagaagt acaagtgcag ccgatggtga
    68161 ctgaggtccc tgtcgccgcc gctgtcgaac ctgttgttag cgcgccagtt gttgaagaaa
    68221 tggctgaagt cgtagaagcc cccgtaccgg ttgccgaacc gcaaccggaa gtggttgaaa
    68281 cgacgcatcc tgaagtgatt gccgccgcgg taactgaaca gccgcaggtg attaccgagt
    68341 ctgatgttgc cgtagcccag gaagtcgcag aacacgcaga accgatggtt gaaccgcagg
    68401 aagagacggc agatgcagat attgaagaag ttgccgaaac tgctgaggtt gtggttgctg
    68461 aacctgaagt tgttgctcaa cctgccgcgc cagtcgtcgc tgaagtcgca gcagaagttg
    68521 aaacggtggc cgcggttgaa ccagagatca ccgttgagca taaccacgct accgcgccaa
    68581 tgacgcgcgc tccggcaccg gaatatgttc cggaggcacc gcgtcacagt gactggcagc
    68641 gccctacttt tgccttcgaa ggtaaaggtg ccgcaggtgg tcatacggca acgcatcatg
    68701 cctctgccgc tcctgcgcgt ccgcaacctg ttgagtaata attagctcaa agtaatcaag
    68761 ccctggtaac tgccagggct tttttatttc atgtttgaat caacatatta aaattcatca
    68821 aatggcatcc ttgctaacca acaatgcaaa ataggcaaaa aaaatgcccg ccccggcgat
    68881 cccagagcgg gcagacaaaa catacccagt ttcatgatat gttttaaagc gagcttattt
    68941 gttgagctgg aagagcgaca atccctgcat atcggtaaat gctttatacg atgcctgcaa
    69001 tgccgtttgc tgcatgatgt aagatgaaat agttgcattc cagtccacat caaccagatc
    69061 gctcatctgc tgcgtttgcc ctaaagcgcg atcgctacct aaggaatcca gcgactccag
    69121 tttgttcagc tgcgtgccta attccgcgcg aacggtaagg acattattca gcgagttttt
    69181 cagtccacgg ttggttttat ccaatgccgc agcggcgatt tctttatccg cttcgctatc
    69241 cgcgaccggc gttttcaggg ctgcgatggc gctatccagc atggcaaaaa gattggtttc
    69301 agaagcgcta ccgtctggtt ccgctaccgc gttactggta atactgtcga aaattttgtc
    69361 gcccgtatgt cctatcacca tcgaacgcga agcatcgacc tgttgcttaa tactttctgt
    69421 tccgccaaca taatcaccgt tagcctcact aaacggcgca gtctctgttt tatatgtagc
    69481 gcccattacc gtcagtggtg ttcgccagat tcagcaactg gtcacgaagc ccctgaatat
    69541 ccgtagccag cgaggcccgg tcatcgtcac tcaaggtgcc attgctggcg tagacaattt
    69601 tttcctgagc attctggata gcagtggtga cctggctaag tacactctct tccagtgaca
    69661 ctttttgagt ggcgaaagta cgtgccagcg tgtactggct gttttgcgcc tgtgcctggg
    69721 agagaacaac agcttgtgat gcagcaatgg gatcgtctga agggttaacg actcgcttac
    69781 ccgtcgacat ctgctcgccg tacttcatcc attctgcctg agaattggtg atgccgcgca
    69841 tgttttgctg gtacatcatc tgggtactga aacgcatttt atctcccctt agcgaatgtt
    69901 aatcagcgca tcaaaaatcg cgtttgccgt ctgcagaacc tgcgcattcg ccaggtaata
    69961 ctgctgaaaa cgttgcagat ttccgtactc ctcatcgaga ttgacaccgg aaatcgactg
    70021 ctgctgattg gaaagctgcg tcaccacatt accttgcgtg gtgctactgg ttttcaacgt
    70081 cgcggtttta ttaccgatat cactcactaa cgaggcataa gcgtcgttaa aggatttcgc
    70141 accgcccacc gttttactgt tgctttgcag atccagcagg gcctgaccgt tgcggttgtc
    70201 gctatcaccc gcatcttctt cgctcgccat cgcgattttc gcttcgtcgg tgattaatac
    70261 atccatgttg acgatggcgt cacttactgg tttcagcgtg aagctgtcgt taacggcagg
    70321 cgttcctgta aacgtcaact ccagaccatc aaatgccact ttaccgttgg catctggtgt
    70381 cgccgtaaaa gtggtattgc tggcaaggcg ggtgacctgc cactgattat tatcgaacga
    70441 gattttgtaa tctgtcgcca gtaccacgga ggcatcagtt accgtggcac cgatcgcaac
    70501 gtcacctttg tttttcgtgt tttgcagaac cgcgggctta ccgatagtaa agaaatcttc
    70561 accagcatcg ccgttagcat caaatccggc tttgtgttgg ctgttgaaag cctcggcaaa
    70621 tgccagcgcc agttgtccaa gcgtattacg cgtctggtcc agatcctgag aacggaatgt
    70681 cagaatgccg cccagcgacc cggtattcag taatttctcc gggatctcaa tattgcctgc
    70741 cgtcccatca acgtaagcga cagtcgtacg agaagggtcg gcgctggaag gaactgccgc
    70801 cagttgccgc gccgtacttc cctgaaccag tgagtaacca ttggcatcgt gatgttataa
    70861 gtaccgccat cctgaacgct gacttctacg ccaacaatct ggtttaattc gctcaccagt
    70921 tgatcgcgtt gatccagcag attgttaggt gacgcccctg cccccacgcc tgtcaggcgc
    70981 gagatttgat cgttcaggct ggcaatttgt ttagcgtaat tgttgatctg atcaacgctg
    71041 gcacctatcg cgatattgac ctgtttgtcc tggtcgcgca gatattgatc ggtggtttta
    71101 aactgattca ccaacccttc tgatttccca atcagcgcct ggcgcgccgc cgggtcttcc
    71161 gcgttactca ccagcgtttg caggctggtg aagaaatcct gcatctgtgt tgccagcgaa
    71221 gaggtactgg tggagagcat attgtcgatt ttcgacatct gctcatagcg ggcggtcagg
    71281 ccgctacttt gcgtctgcgc agcacgtaac tggttggtaa taaacgtatc atactcacgc
    71341 tgcacaccag aaacgtagac gccattgcca acccagccgc cagcgcccaa cgtgctattg
    71401 gcctgcgcca taatagtggt ttggcgggta tatccggcaa cgttatagct ggagatatta
    71461 ttacttgccg tatttaacgc cgcctgggcc gcgttcagtc cgctcatggc gttattgatc
    71521 aagctggaca tggaggttcc ttataagcct tcaattacag agtattatcg gcagcgaccc
    71581 gccggacttg agttattcag aacagattat caatgttcat gctgtaggtt ttgctcacct
    71641 tgtcgcttat cgatttcatc tgctggatca tgttggtgag tttacgggca tagtgaggat
    71701 cggtagcata gcccgcgtcc tgtaggacct gcgccccctg ttccgcactc gcggcggtcg
    71761 tcacggcggc gtagcgcggg ttacgtgtta acagcccaac gtaatccgac aatgcttcca
    71821 gatacgagct gtagacccga aacttcgctt ttactttctt cgcttcgccg ttttcatatt
    71881 cagtcgtggt gatttcagtg acttgccctt tccagttgcc agaggctttg acaccaaaca
    71941 ggttatagct tggctcgccg ttttcgcggc ggatttgccg ttgcccccaa ccagattcaa
    72001 gcgcagcctg agcgaggatc aaatgatgtg gcacaccgct ttgctggctt gccagttggg
    72061 cgggcagcga gagttgcgcg aggaatgctc tactgtcacc cggcagcgaa tcatcgtagt
    72121 tacgtggtac ggccttttgc accagctgcg aaagcgtctg gttttgataa cgcaccacgg
    72181 tttcgagcgg gaatttcatc ggtgctgctg gcgtggactc ctctggcaat ggttgttctg
    72241 gcgtcatctg tttaaccatc atctctgcaa gccccagacc tttgcccgcc gtcatctgtt
    72301 gggcaatttg ctggtcatac atactggtat acagacgagt gtgctcgctg ctgaacaggc
    72361 catcttttgg taaagcgtcg cgcatgcttt tcaacatcat ctgcacgaac atcccttcca
    72421 cctgacgggc caccggacgg atatttgccg ccggatcttc gctcgctttc gcctttagtt
    72481 cgttgagtga ttgcgcatcc caggccgcac ttgccagtag tttgctgtcg ctgatcatca
    72541 gatgatttcc agttttgccc gcagacatcc cgcactttgc attgattgca aaatagacat
    72601 cagatccatc ggcgtagcgc ccagcgcatt gagcgcacgc accacgttat tgaggctggc
    72661 gctggaacgt acgctttgca gcgaaccgcc gctctggcgt aaatcgatct gcgtttgtgg
    72721 agtaacaaca gtctgtccac caccaaacgg tgtatctggc tggctgacat tggcctgacg
    72781 attaactgtt actgagagat ttccctgcgc taccgcgcag ctgtcgaggg tcacttcgcg
    72841 attcatcacc accgaaccgg tgcgcgagtt aatcactact ttagcgtcct gcggggtgac
    72901 attaacctgc atattctgga tatcggcaag gaaacggacc tgggaactgt tgccacttgg
    72961 tacgcgcacc tgaatagtcc gcgcatctaa cgcggtggcg ctgccatatc cacgcacgcg
    73021 gttgatggtg tcagcgattt gctgcgccat gctgaaatct tcgtcgttaa gttgcaaatt
    73081 aagggtattc ccgacgccaa actggctggg caattcacgt tcaataaccg caccattggt
    73141 gatccgtcca ccgttcagtt ggttaacctg aacactgcta ccgcctgcgg aggctcctgc
    73201 gccgccaacc agaatattgc cctgcgccag cgcatacacc tgactgtcaa cgcccttaag
    73261 cggagtcatc aacaacgtac ctccacgcaa gcttttggca ttacccatgg aagaaaccac
    73321 cacatcgatg gtttgcccct gacgtccaaa cggaggaagt gacgctgtca ccattaccgc
    73381 agcgacgttt tttagctgca tattggtgcc cgtcggaacg gtaattccca gctgtgagag
    73441 catgttatta agcgtttgtg tggtaaacgg cgtctgggtt gtctggtcac cagtgccatc
    73501 cagccccact accaagccat agccaatcag tgagttttgc cttaccccct gaacactggt
    73561 gagatcgcga atacgctcag cctgagccgc cgtcgtaacc agtagaagaa ttaatgcaga
    73621 gagaaattta atcaccacag cctcacttac attggcgaca ggttaaggaa gaaacgctgc
    73681 aaccagccca tattttgcgc ttcgttaatg tagccattgc ctacgtattc aatacgcgca
    73741 tccgccacct gagtagacgg tacggtattg ctgccgctga tagtgcgtgg attaaccacg
    73801 cccgagaagc gaataaattc ggtaccctga ttaatggcaa tctgtttttc acccaccaca
    73861 tgcaggttgc cgttgaccag tacctggtca accgtcaccg tcaacgtgcc gctaaaggta
    73921 ttgctggcat tggccccgcc ctttccgttg aacgtgttac caccggaggc ttcgacatcg
    73981 gcacgagcgt taccaaacag cccctgcaaa tagcgcggca cagtatcaaa gccaaaatta
    74041 gttttaccgt cacggctggc attcgcagag gagcttttgc tggcgctgac gttctcctgc
    74101 aacacgatgg tcagcgtatc gccaatattg cgtggtcgac gatcttcaaa cagcggttga
    74161 tagccatagt taatcggctg agcagactgg aaaatagaac cgttggcgac gggcgtcgga
    74221 ccgggaaccg gttgtgcact ggtcgccccc tgcaccagcg gcgtggaggg tatccaggcg
    74281 cagccggtta gtgaaagcac caacaagctg gaaatggcat aagtatgcgc agcgtttttt
    74341 tgcatggcta tcatcttcaa aaatcagtaa accggtgaac ctgccaccgg ttatgcctta
    74401 gagttgcgtc agtttttgca gcatctgatc ggtggtggac accgctttac tgttgatttc
    74461 gtaagcgcgt tgcacctgaa tcatattgac cagttcttcc gccacgttga cgttagacgt
    74521 ttcaacatac ccttgataca gcagtcccgc gccgttcagg cccggcgtgc tttcgttcgg
    74581 tgcaccggag gattgcgttt cggtgtagag gttttcgcca atgctctcca gtccggtatc
    74641 attcataaag gtggtgagat tgagctgccc aacctgaacc ggagctgcct ggccttgttg
    74701 ggttacgctg accacgccat cacgaccgat ggtgatactt aacgcattcg ccggaatggt
    74761 gatcgctggc tgcacctgaa aaccaccagc cgtcaccagc tgcccgttct gatccacctg
    74821 gaaagagccg tcacggatat aggctgatga gccatctggc aacattacct ggaaaaagcc
    74881 ctgcccttta atcgcgacat ctttgctgtt attggtctgc gacagattgc cctggctgtg
    74941 taagcgttca gtggcgaccg ggcgtacccc cgtgccgatt tgtaatccgg agggtaaggt
    75001 ggtttgttcg gaagactgtg cccccggctg gcgaatggtt tgataaagca gatcttcaaa
    75061 caccgcgcgc tgacgcttaa aaccgttagt actgacgttt gccaggttgt tggcaatgac
    75121 gtccatattg gtttgctggg cgtcaaggcc cgttttggcg atccataatg aactgatcat
    75181 ataatgtcct gtagtggatt gctgacaagc agagcagaag cacgcgacag tgtgaatttc
    75241 ccctcaccct aaccctctcc ccaaaggggc gagggaatag tccgcgcgct tttattgcaa
    75301 tgtcatcaat cgttcaatta actcatcgac agcagttggt tggcacggcc tgcgttatca
    75361 tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc gcgcgctggc aatcatgtcg
    75421 ctcattgccg caacggcatt gacgttactg ccttccagaa cccccgacat cacacgcaag
    75481 gttggatctg cctgcagtac cggccctcgc gtggcctggg tttctgcgct taaacgaaaa
    75541 atgccgtcgt caccgcgctg cacttcgctg cccgtggctt tcaccagttt cagacgccct
    75601 actggcgcaa ctgtatttgc cggatcgccc ggattgagcg ccgagattgt gccatcggca
    75661 gcaatcgtga tttccgcccc ttccggcaca gcaattggcc ccgcctcgcc tatcaccgga
    75721 tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa tgctgccatt acgcgtatac
    75781 ccttcgctgc cgtcagcggt ctgcacggcc agccatcctg ctgcaacgca acgtccagcg
    75841 ggcgagaggt gtaatccatc ttgccgggcg tcatgtctgc acccggtgtt gacgccgtta
    75901 ccagcgtgcg cgtgggcagt gaaagccctt ccaccggcac tgcgcgcaag gcgtttaact
    75961 gcgcgcgaaa accgggcgta gaggcattgg ccagattact ggcggttacc gcctgttgat
    76021 tcagcgtctg gctggctgct cccatcgcag tataaattgc gtgatccatt gagctatccc
    76081 gtcagcgatt agcgtaagtt aacccgcgtg ttgaggatct ggtcctgggt tttgatggtc
    76141 tgggcgttag acttatagtt acgctgggca acgatcatat tgaccagttc tttactgaga
    76201 tcgacgttgg acgcttccag cgcaccgttg gtcagagtgc caaagtttcc cgtcccggct
    76261 gtccccaaca gcgccacgcc agaagattgc gtcgcagacc agacgttgtc gccttcggat
    76321 gccagacctt cgttgttggc aaagttcgcc agtacaatct gccccagcag ttgggtttgt
    76381 tcgttggaat tgttgccgac aaccgtaccg tcatcattga tttgataact caccagatcg
    76441 cccggtttgt agccattctg ggtggttgcc acaatgttgt tagcgccggt tgcgatatta
    76501 ttcgccatcg caccatccac taatgtgcca ttagcattaa attccagtgt tgtcgctgtc
    76561 ttcgcaatgc tgtttggatc actgctatcc tgggtgtaga cctgccagtt attatccccg
    76621 gtcttcacaa aatagacgct catgtcatga gcattaccct gactgtcgaa aacagtcacc
    76681 gaaccttttt tgttatagct atccgcattg ctggcgctga atggcgtaac agtaggaagc
    76741 ggatcactgg aattcaggtt gatctgcatc gacgccgtgg tggtagtttt cgctgccatc
    76801 agggtattcg ggatcgaaat attggtcgga ttcgcccctt gctgaatagt cggcggcgta
    76861 ccggttaccg ggtaacccgt cagctgtaaa ccttgcgtat tcaacaggtt acggttttca
    76921 tccagcttaa attgtccgtt acggctgtag aacaccgaac cgttgctgtc taccagacgg
    76981 aaaaaaccgt tctggctgat agcaacgtcc agacctcgcc cggtgttggt ggtcgtgcca
    77041 tcggtaaagt cctgagtgat accggcaact tttaccccca gtcccacttt cgaaccggca
    77101 aacatatcgg caaaagaggc cgtgcctgat ttaaagccgt aggtggcgga gttggcgata
    77161 ttgttgccaa taacatcgag gttggtggcg gcagcgttta atccgctaac cgcttgagaa
    77221 aaggccatga ctgactcctg aaatgtgaag gcttaaatta tctgccgtac ttcgtcgagg
    77281 gtggtggtgc cgtaagtgcc gagatccagc gtattaccgc tgttgccgcg gatcacaccc
    77341 tgcaccagag caaactgcag cggctgggca accagttgtg taccaccgtt actggcgcta
    77401 atcgctacat tgtaagaacc gttcggcgca gtgctgccat cagtcaacga accgtcccag
    77461 gtgaaactgt gaactccggc ggtcagttca ccaatatcaa tggtgcgcac aaccgcgcca
    77521 tttttatcgg tgatggtggc cgtaactttg tctgccgcct gttgcagctc aacaccaaac
    77581 ggcgtggtcg tggtcacagc cccttcttca ctgccggttc ccgcaagaac agttgtgccg
    77641 gggatcatca cgccgtgacc gatcaggtta ctggcctgta acgactggct gttatcaatc
    77701 tgtccggaaa tagatccgag cgtggtattg agtttttcaa tcccgctgac cgtgctgatt
    77761 tgtgccaatt gcgacgtcag ctcgttgttt tccattggat tggtcgggtc ctggtttttc
    77821 agctgcgcca ccagcaaagt cagaaaactg ctttgtaaat ctgcggcgtt gctgcccgtg
    77881 agcgaactac tgctggtggt actgacgccg gtatttgtcg gatcggtggt ggttaccgca
    77941 atggacatag ctttctcctt tattgaccga gcgtaagggt tttcagcatc atgcttttca
    78001 ccgtgttgag cacttcaaca ttggcctgat agctgcgtga cgccgacatg gtgttaacca
    78061 tctccccgac gacatcaacg ttcggcattt ttatgtagcc ctttgcatct gccagcggat
    78121 tacctggttc ataaaccagt ttgtccgggg cctgactttc ttaacatcgg caacctttac
    78181 gccgcctgtc gcagcacctg gtgcagcgtt aacctggaat accacctgtt ttgcccgata
    78241 tggctgtcca tcaggaccgg tcacgctatc agcatttgcc agattactgg ccgccacgtt
    78301 caggcgctgg gactgggcag ttaacgccga cccggcgata tcaaaaatat tcagcagtgc
    78361 catccgttaa tttccgctct gtaaaacgtt catcatgcct ttgatttgcc cgctcaacgc
    78421 gctaaggctc atctggtatt gcaggctgtt atcggcaaac tgggtgcgtt cgcgatccat
    78481 atcgacggta ttaccgtcaa gcgaaggctg gtccggaata cggtattgca gttctgcggt
    78541 aggaggcgtc agcgcctgcg ccgggatgtg ctgcgttgag gtcatcgtca gtgcaaccac
    78601 actggttgca tcccgtccac gttgcatgac ttttttaagt tcactggcaa aatcgatatc
    78661 gcgcgcctga taaccagggg tatcggcatt ggcgatgttt gctgccagca cttcctgacg
    78721 ctgggcgcgc agattgagcg cctcttgttg aaaacgtaag gcggcgtcga gcttatcgag
    78781 catatctcct ccgcaggtat caaaattctg ccatcagctt aaatgccttt acgcgcgcgt
    78841 tatcggcgga ataaacgcaa aatgggtcgc tatttatgcc gttgatggtc attgcggaca
    78901 ggtacaattc acgttgtaga aatggctggg ggcgaaaatg ctgacaataa aacgtagcgt
    78961 ggcgatcatc gcgatactgt tcagtccgtt aagtacggcg agcaatctca catcgcaatt
    79021 gcacaacttt tttagcgccc aactcgcggg ggtaagtgat gaggttcgtg tttctattcg
    79081 tacagcgccc aatctactac cgccatgcga gcagccattg ctttcgatga gcaataattc
    79141 ccgcctgtgg ggcaatgtga atgtgttggc acgctgcggt aacgacaaac gatatttaca
    79201 ggttaatgtg caggccacag gaaattatgt ggttgccgcg atgcctattg tgcggggagg
    79261 aaagctggaa gctggcaatg tcaaactgaa acgtggacgc ctggataccc tgccaccgcg
    79321 tacggtgctg gatatcaatc aacttgttga tgccgttagc ctgcgcgatc tatcacccga
    79381 tcaacctatc cagttaaccc agtttcgcca ggcatggcgg gtaaaagcgg gacaacgcgt
    79441 caatgtgatc gccagcggtg atgggtttag cgccaacgca gaaggtcagg cgctgaacaa
    79501 tgcagccgtc gcacagaatg cgcgggtgcg catggtatcg gaacaggtag tcagcggcgt
    79561 tgttgatgca gatgggaata ttcttataaa cctgtaagct gttaaagatt acccgtccct
    79621 tgccgataaa taagcaacac atgataaaag cgccctcaat gaggaataaa ccatgagtat
    79681 tgatcgcact tcgcctctga agcctgtaag caccgttcaa ccgcgcgaaa ccaccgacgc
    79741 gccggtaacg aacagccggg cggcaaaaac aaccgcctcc accagcacca gtgtgacgtt
    79801 aagcgacgcg caagcaaaac tgatgcaacc cggcagcagt gatatcaatc ttgaacgtgt
    79861 cgaagcgtta aaactggcga ttcgtaacgg tgaactaaaa atggacaccg gcaaaattgc
    79921 cgatgcgctg atcaacgaag cgcagcaaga cttgcagagt aactgatcgt atgacacgtc
    79981 ttgcagagat cctcgaccag atgtccgctg tgcttaacga tctcaaaacg gtaatggatc
    80041 aagagcagca acatctctct atggggcaga tcaacggcag ccagttgcaa tggattacag
    80101 aacaaaaaag ctcactgctg gcgacgctgg attacctcga acagttacgc aagaaagaac
    80161 ccaatacagc aaatagcgtt gatattagtc aacgctggca ggaaattact gtgaaaacgc
    80221 agcaactacg ccaaatgaat caacataacg gctggttact ggaaggacag attgagcgca
    80281 atcaacaggc gctggaaatg ttgaaaccgc atcaggaacc gacgctatat ggggcgaacg
    80341 gtcagacctc aacaccccat cgcggcagta aaaagatttc gatctgaata ttatctccgg
    80401 cctgcactgc aggccggaat gcattgttac accgtccggc gggcaaattc tttaactttg
    80461 aagcccagta ccgccagtgc agcgaagtac gcggcaatcc ccgccagcac gaccgccatt
    80521 aaacgcagta agcgccaggg catggtaccc aatgaccact ccggcatgat atgtaacata
    80581 cctaaaagca cgccagacat caccagtacc gccaccacca gacgcaacag aaacgccatc
    80641 cagccgggtt gcggagtaaa gattttctgc ttacgcaact gccagtaaag cagcgaagca
    80701 ttcagacacg ccgccagacc aatagaaagt gacagcccgg catgtttcaa cggaccaata
    80761 aacgccaggt tcatcaattg cgttaaaatc agcgtaacga tggcaatttt cactggcgtt
    80821 ttaatgtcct ggcgggaata aaagccagga gccaacactt tcactacaat caggccaatc
    80881 aaacccaccg agtaggcaat taacgcccgc tgggtcatca gcgcatcaaa cgcggtaaat
    80941 ttaccgtact ggaacagcga aacggttaac ggacctgaaa gaatgcccaa cgcaaccgca
    81001 ctcggcagtg ccaacaggaa acaaagacgc aacccccagt ccatcaaacg gttgtattca
    81061 tcatgattgc cactggcaaa acttttcgac agcgacggca gcaaaatggt gccaagcgcc
    81121 acgcccagca caccggaggg aaactccatt aagcggtcgg cgtaatacat ccaagacacc
    81181 gaaccggaag caagaaacga ggcaaaaatg gtgttgatga ttaaggagat ctggctcaca
    81241 gagacgccaa ggatcgccgg tcccatctgt ttcaccacgc gcattgctcc agcatcgtgg
    81301 aagttaatgc gcggcaggac cagcatgccg atcttcttca ggtgcggtag ctgatacacc
    81361 agctgcagaa tgccgccgac cgtaacagcc cacgccagcg ccagcaccgg tgggttaaag
    81421 tacggtgcgg caaacagcgc gaaaccaatc atgctgatgt taagcagtgt tggcgcaaac
    81481 gccggaatcg agaagcggtt ccacgtattc agaatcgctc ccaccagcga cgccagggag
    81541 atcagcaaga tatagggaaa ggtaatcttt agtagctggc tggtcagggc aaatttgtca
    81601 gctgtgtcag cgaagcctgg cgcggtcacc atgatcaccc acggtgcggc gagcatgcca
    81661 gcgaccgtca caaccgccag cgcaagtgtc agcaggccag aaacataaga gacaaagacc
    81721 cgcgtggcgt cttcaccctg cttactttta tattccgcca gaatcggtac aaatgcctgg
    81781 gaaaatgccc cttcggcaaa gatacggcgt aacaagttag gaagtttaaa agcgacgaaa
    81841 aaggcgtcgg ttgccatccc tgcgccaaag attctggcga caattgcgtc tcgtgcgaag
    81901 ccaagcacac gcgaaaacat ggtcatcgag ctgacggcgg ccagcgattt taataaattc
    81961 atcggtgttc taatccggac ccacgggcaa aacgcctgca aggcaggcgc tgaaagaggc
    82021 gaatagtcta cctagattat ggtgaattgc taccgccaga tgttacaggg tttattcact
    82081 catcgcatcg cgccagatct tgtcaacgat acgttgcgcc agcactgcct attcgccggc
    82141 ggtttgcgga actgtctgat tttacacgca ttcaatgaag tgccgcgcac agccgacaaa
    82201 tccacgttgc tcaagagtgc tctgccaacc aggaatcggt ttattcacta caccctgccc
    82261 gcgctcctca cgccattcgc gcatatccgt aatgtcgatg agcgcaccgt cagtcacggc
    82321 ctgcacggtt tcacgctgac ttccggcacg gcgatgcata caagtggtga tctgcaaagg
    82381 accggccgaa aaatggtgct cggcaaacag catttcgcca gcgtcgttag tcagtagcgt
    82441 accgccatcc agagaggctt tgccgcccga caaccacagc gcggtatcca ccacatgcag
    82501 ataatcatcc agcaacgtga aataaagatc gtgtggcccg acgctattgc tacgatgttt
    82561 atccattctt agcgaggctg cggtggcgag ttgcgttttt aactcaccgt agagtggtgc
    82621 gaaacgacgg ttaaaaccga ccatcaacgt cagttttttc cgcgccgcca gttccaccag
    82681 cctttcagca tcgcgcagat tttctgccag cggtttatcg acacagacat gtacccccgc
    82741 attgagtaac gtactgacca cgtcaaagtg gctggcggtg ctggagtgca caaaaaccgc
    82801 atcacaactg gcggcaaggc tggataacga atcggcataa ggaatgcgcc agctgtcaca
    82861 aattggcagg gctttggcgc gcgtaggcga ccaggctcct tgtaacgtcc agtcagacgc
    82921 tgccgccagc accggtaacc acgctttttg cgcaatgccg cctaatccca ctacgccgat
    82981 acgtaatttt ttcactttaa tctcccagat gggccagcaa cgaatcaaga cgttgtttca
    83041 gttctgccac ttcgatttcc agggcttcga cgcgggtctg taaatcaccg tcaaccatgt
    83101 tcgacatagc cgttaccgcc ggctgatctt caacctcacc actgaaaaga tgcatgtagc
    83161 ggctttcgcg tttacccggt tcgcgggcca gacgcaccac aaaaggacca tcttcgcgat
    83221 tcgccagttg ttccagcgtc gactccactt ccgccatatc gctgaattca tacattcgcg
    83281 cggcgcggct gcgcagttca cccggcgtct gggcaccacg caataacaac gtggtgatta
    83341 acgccacttc tgcggcgctc agtttcagat cgccaaattc tgaattacaa aaacgttgct
    83401 catatttggt gacccgatta ccaaaaccgc tcactgtgcg tagataatga cgtttgacca
    83461 gattatccag ctgttcctgc acttcggatt cactcagatt catgaccggt tcacggttcg
    83521 ttttctgatt acaggccgtg actacaccat tgactgagag cgggtattgc tccggcgtgg
    83581 tcacctgctt ttccagcaag caaccaatta cgcgagcttc cagggcagta agttgatatt
    83641 tcatgagatt ctccttagcg gccgggcgtc cagtctgggg tagttaatgc cgtcagtacg
    83701 tgatcgcgcc attgtccatc aatcaacaga tagtctttcg catagccttc tttttcaaaa
    83761 cccagtcgcg ccagtaaatc accgctgcgt ttattgtgcg gcatataatt agccataatg
    83821 cgatgaatat gttgggtgcg ctgcatataa cgaatggctg cggtcagggc ttcaaacatg
    83881 agtcctttgc cctgccattt ttgcccaatc gaataaccga gatagcaggc atgaaaagag
    83941 ccgcgaacaa cattggaaaa attggcaacg ccaataatct ctttttcgtc cgggtcgaat
    84001 aagccaaagt agaaagctga accttgttta tgaaattcgt taatcatccc tagcctggcc
    84061 tgccagcctg atggataaca gtggctttcg tcgcgcactg gctcccaggg cttgaggaaa
    84121 tggcgattct ctgcgtaata atccgccaga cgccaggcat cacgatcatg caccagacgc
    84181 acgaccagtc ggtctgtggt taagcgcact tttggcacgt tactgcgata gccaaacatt
    84241 ctatacctac tccttcccgt aacgtcttta atacgctgta agtattacta tacctgcgca
    84301 accagaccct gtgaaaagtc caaaatgcca ttttctgcat tattcacata tttgataaaa
    84361 actctctatc aaagcgacgt tttgataaaa aaatactgtc cccgaagtga tgggaaaagt
    84421 cttagcggca atgcagaata gaaagtgctc atcttttatt tcccgggagg ggaaatgtcc
    84481 cgcgtatcgc aggcgaggaa cctgggtaaa tatttcctgc tcatcgataa tatgctggtc
    84541 gtgctggggt tctttgttgt cttcccgctg atctctatcc gcttcgttga tcaaatgggc
    84601 tgggccgccg tcatggtcgg tattgctctc ggtctacgcc agtttattca gcaaggtctg
    84661 ggtattttcg gcggcgcaat tgccgaccgc tttggtgcca aaccgatgat tgttaccggt
    84721 atgctgatgc gcgccgccgg attcgccaca atgggtatcg cccacgaacc gtggctattg
    84781 tggttttcat gcctgctctc gggactcggc ggcacgttgt ttgatccgcc gcgttcggcg
    84841 ctggtggtga aactaatccg tccacaacaa cgtggtcgtt ttttctcgct gttgatgatg
    84901 caggacagtg ccagtgcggt cattggcgca ttgttgggga gctggctgtt gcaatacgac
    84961 tttcgcctgg tctgcgccac aggggccgtt ttgtttgtgc tatgtgcggc gttcaatgcg
    85021 tggttgttac cggcatggaa actctccacc gtacgcacgc ccgttcgcga aggcatgacc
    85081 cgcgtgatgc gtgacaagcg ttttgtcacc tatgtcctga cgctggcggg ttactacatg
    85141 ctggctgtac aagtgatgct gatgctgcca attatggtca acgacgtggc tggcgcgccc
    85201 tctgccgtta aatggatgta tgccattgaa gcgtgtctgt cgttaacgtt gctctaccct
    85261 atcgcccgct ggagtgaaaa gcattttcgt ctggaacacc ggttgatggc tgggctgttg
    85321 ataatgtcat taagcatgat gccggtgggc atggtcagcg gcctacaaca acttttcacc
    85381 ctgatttgtc tgttttatat cgggtcgatc attgccgagc ctgcgcgtga aaccttaagt
    85441 gcttcgctgg cggacgcaag agctcgcggc agctatatgg ggtttagccg tctgggtctg
    85501 gcgattggcg gcgctattgg ttatatcggt ggtggctggc tgtttgacct gggcaaatcg
    85561 gcgcaccagc cagagcttcc gtggatgatg ctgggaatta ttggcatctt cactttcctt
    85621 gcgctgggtt ggcagtttag tcagaaacgc gccgcgcgtc gtttgctgga acgcgacgcc
    85681 tgatttgata ctcccccctg actcctccat actgaaagtg aactgacagc caaatggagg
    85741 agtcacgtga agctatacat ttacgatcac tgcccttact gcctcaaagc ccgcatgatt
    85801 ttcggcctga aaaatatccc cgtcgaatta catgttctgc tcaacgacga cgcagaaaca
    85861 cccacccgga tggtcggtca aaaacaggtt cccattctgc aaaaagatga cagccgctat
    85921 atgccagaaa gcatggatat cgttcactat gtcgataaac tcgacggcaa accgttactg
    85981 accggcaaac gttcccctgc cattgaagag tggctgcgca aggtcaatgg ctacgtcaac
    86041 aaactgctgt tgccgcgttt tgccaaatcg gcatttgatg agttttctac tcccgccgcg
    86101 cgcaaatatt tcgtcgacaa gaaagaggcc agcgcgggta attttgccga cctgctggcc
    86161 cactctgacg gtctgattaa gaatatcagc gatgatttgc gtgcgctgga caaactgatc
    86221 gtcaaaccga acgccgtgaa tggcgaactt tcggaagatg atattcagct atttccgcta
    86281 ctgcgtaatc tgacgctggt agccggaatc aactggccaa gccgcgttgc taattaccgc
    86341 gataatatgg cgaaacagac acaaatcaat ttgttatcat caatggcgat ttaatcctga
    86401 cccgccgcgg cagcctgtat tcgcgggttg ccgccgtttt cagttttttc tgctggcgag
    86461 tttatcgctg gtgacacatg ctgcgttgtg tcaggatatt gacgcagatt tcatgtcgag
    86521 gactaccatg aacaagtttt tatttgccgc tgcattgatc gtcagtggcc tgctcgttgg
    86581 ctgtaatcaa ctcacccaat acaccatcac cgaacaagaa attaaccagt cgcttgcgaa
    86641 acataataat ttctcaaaag atatcggttt acccggcgtg gctgacgccc atattgttct
    86701 gacaaacttg accagccaaa ttggtcgcga agagccgaat aaggttaccc taaccggaga
    86761 cgccaatctg gacatgaact ccctgttcgg tagtcagaaa gcgaccatga aactgaagct
    86821 gaaagcgctg cccgtgtttg ataaagaaaa aggtgcgatc ttcctgaaag agatggaagt
    86881 ggtcgatgcg acggtacaac cggaaaaaat gcaaacggtg atgcaaacgt tgcttcccta
    86941 tttgaaccag gcattacgca attactttaa ccagcaacct gcttacgtcc tgcgcgaaga
    87001 tggcagtcag ggcgaagcaa tggcgaaaaa actggcgaaa ggcattgaag tgaagccagg
    87061 cgaaattgtc attccgttta cggattaatg acgagggcgc attcgcgccc tttatttttc
    87121 gtgcaaagga aaacgtttcc gcttatcctt tgtgtccggc aaaaacatcc cttcagccgg
    87181 agcatagaga ttaatgactg caccatccca ggtattaaag atccgccgcc cagacgactg
    87241 gcaccttcac ctccgcgatg gcgacatgtt aaaaactgtc gtgccgtata ccagcgaaat
    87301 ttatggacgg gctatcgtaa tgcccaatct ggctccgccc gtgaccacag ttgaggctgc
    87361 cgtggcgtat cgccaacgta ttcttgacgc cgtacctgcc gggcacgatt tcaccccatt
    87421 gatgacctgt tatttaacag attcgctgga tcctaatgag ctggagcgcg gatttaatga
    87481 agccgtgttc accgctgcaa aactttaccc ggcaaacgca accactaact ccagccacgg
    87541 cgtgacgtca attgacgcaa tcatgccggt acttgagcgc atggaaaaaa tcggtatgcc
    87601 gctactggtg catggtgaag tgacacatgc agatatcgac atttttgatc gtgaagcgcg
    87661 ctttatagaa agcgtgatgg aacctctgcg ccagcgcctg actgcgctga aagtcgtttt
    87721 tgagcacatc accaccaaag atgctgccga ctatgtccgt gacggaaatg aacggctggc
    87781 tgccaccatc actccgcagc atctgatgtt taaccgcaac catatgctgg ttggtggcgt
    87841 gcgtccgcac ctgtattgtt tacccatcct caaacgcaat attcaccaac aggcattgcg
    87901 tgaactggtc gccagcggtt ttaatcgagt attcctcggt acggattctg cgccacatgc
    87961 acgtcatcgc aaagagagca gttgcggctg cgcgggctgc ttcaacgccc caaccgcgct
    88021 gggcagttac gctaccgtct ttgaagagat gaatgctctg cagcactttg aagcattctg
    88081 ttctgtaaac ggcccgcagt tctatggctt gccggtcaac gacacattca tcgaactggt
    88141 acgtgaagag caacaggttg ctgaaagcat cgcactgact gatgacaccc tggtgccatt
    88201 cctcgccggg gaaacggtac gctggtccgt taaacaataa aaatcgtagc ttcctgttgt
    88261 cattaggtta ttttacctgt ataaataacc agtatattca acagggggct attatgcgaa
    88321 ttgaagtcac catagcgaaa acttctccat tgccagctgg ggctattgac gccctggctg
    88381 gcgaactttc ccgccgtatt cagtatgcgt ttcctgataa tgaaggccac gtatcggtac
    88441 gttatgccgc agcgaataat ttatcggtta ttggcgcaac aaaagaagat aaacagcgca
    88501 ttagcgaaat tctccaggaa acgtgggaaa gcgccgatga ctggtttgtc agcgaataat
    88561 atgcagtgat ttttgttttt gccggatcgc cccggctttt ttattttact tacgaatttt
    88621 gagtacaaaa tttc
//
LOCUS       DAZOYF010000002        70712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZOYF010000002 DAZOYF010000000
VERSION     DAZOYF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.002"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..795
                     /locus_tag="ACHUOF_000100"
     CDS             <1..795
                     /locus_tag="ACHUOF_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0609456.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="ACHUOF_000101"
     CDS             806..1897
                     /locus_tag="ACHUOF_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0609457.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHUOF_000102"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHUOF_000102"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR0609458.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHUOF_000103"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHUOF_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR0609459.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="ACHUOF_000104"
     CDS             4859..5254
                     /locus_tag="ACHUOF_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR0609460.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="ACHUOF_000105"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="ACHUOF_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR0609461.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="dolP"
                     /locus_tag="ACHUOF_000106"
     CDS             5874..6449
                     /gene="dolP"
                     /locus_tag="ACHUOF_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR0609462.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="ACHUOF_000107"
     CDS             complement(6563..7603)
                     /locus_tag="ACHUOF_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0609463.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="ACHUOF_000108"
     CDS             complement(7676..8311)
                     /locus_tag="ACHUOF_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR0609464.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHUOF_000109"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHUOF_000109"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR0609465.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="ACHUOF_000110"
     CDS             complement(8937..9380)
                     /locus_tag="ACHUOF_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR0609466.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHUOF_000111"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHUOF_000111"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR0609467.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="ACHUOF_000112"
     CDS             complement(9720..10223)
                     /locus_tag="ACHUOF_000112"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0609468.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="ACHUOF_000113"
     CDS             complement(10217..10741)
                     /locus_tag="ACHUOF_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR0609469.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="ACHUOF_000114"
     CDS             10950..11945
                     /locus_tag="ACHUOF_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR0609470.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="ACHUOF_000115"
     CDS             11954..12832
                     /locus_tag="ACHUOF_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR0609471.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="ACHUOF_000116"
     CDS             12913..13920
                     /locus_tag="ACHUOF_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR0609472.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHUOF_000117"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHUOF_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR0609473.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHUOF_000118"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHUOF_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR0609474.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHUOF_000119"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHUOF_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR0609475.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHUOF_000120"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHUOF_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR0609476.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHUOF_000121"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHUOF_000121"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR0609477.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHUOF_000122"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHUOF_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR0609478.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHUOF_000123"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHUOF_000123"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR0609479.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHUOF_000124"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHUOF_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR0609480.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHUOF_000125"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHUOF_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR0609481.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHUOF_000126"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHUOF_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR0609482.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSAELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHUOF_000127"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHUOF_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR0609483.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="ACHUOF_000128"
     tRNA            complement(27654..27730)
                     /locus_tag="ACHUOF_000128"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="ACHUOF_000129"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="ACHUOF_000129"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR0609484.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31055)
                     /locus_tag="ACHUOF_000130"
                     /pseudo
     CDS             complement(29429..31055)
                     /locus_tag="ACHUOF_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(31514..31600)
                     /locus_tag="ACHUOF_000131"
     tRNA            complement(31514..31600)
                     /locus_tag="ACHUOF_000131"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHUOF_000132"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHUOF_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR0609485.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHUOF_000133"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHUOF_000133"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR0609486.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHUOF_000134"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHUOF_000134"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR0609487.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHUOF_000135"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHUOF_000135"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR0609488.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHUOF_000136"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHUOF_000136"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR0609489.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHUOF_000137"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHUOF_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR0609490.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHUOF_000138"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHUOF_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR0609491.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="ACHUOF_000139"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="ACHUOF_000139"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0609492.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            39902..40003
                     /locus_tag="ACHUOF_000140"
                     /pseudo
     CDS             39902..40003
                     /locus_tag="ACHUOF_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHUOF_000141"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHUOF_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR0609493.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="ACHUOF_000142"
     CDS             complement(41212..42177)
                     /locus_tag="ACHUOF_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0609494.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHUOF_000143"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHUOF_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR0609495.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHUOF_000144"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHUOF_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR0609496.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="ACHUOF_000145"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="ACHUOF_000145"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR0609497.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHUOF_000146"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHUOF_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR0609498.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHUOF_000147"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHUOF_000147"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR0609499.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHUOF_000148"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHUOF_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR0609500.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHUOF_000149"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHUOF_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR0609501.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHUOF_000150"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHUOF_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR0609502.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHUOF_000151"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHUOF_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR0609503.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHUOF_000152"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHUOF_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR0609504.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHUOF_000153"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHUOF_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR0609505.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="ACHUOF_000154"
     CDS             49715..50680
                     /locus_tag="ACHUOF_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR0609506.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHUOF_000155"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHUOF_000155"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR0609507.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHUOF_000156"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHUOF_000156"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR0609508.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="ACHUOF_000157"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="ACHUOF_000157"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR0609509.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="ACHUOF_000158"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="ACHUOF_000158"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR0609510.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="ACHUOF_000159"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="ACHUOF_000159"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR0609511.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHUOF_000160"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHUOF_000160"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR0609512.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="ACHUOF_000161"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="ACHUOF_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR0609513.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHUOF_000162"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHUOF_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR0609514.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHUOF_000163"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHUOF_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR0609515.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="ACHUOF_000164"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="ACHUOF_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR0609516.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHUOF_000165"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHUOF_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR0609517.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHUOF_000166"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHUOF_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHUOF_000167"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHUOF_000167"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR0609518.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHUOF_000168"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHUOF_000168"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR0609519.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="ACHUOF_000169"
     CDS             complement(62547..63476)
                     /locus_tag="ACHUOF_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR0609520.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64151..68611
                     /gene="gltB"
                     /locus_tag="ACHUOF_000170"
     CDS             64151..68611
                     /gene="gltB"
                     /locus_tag="ACHUOF_000170"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR0609521.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68624..70042
                     /gene="gltD"
                     /locus_tag="ACHUOF_000171"
     CDS             68624..70042
                     /gene="gltD"
                     /locus_tag="ACHUOF_000171"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR0609522.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70226..70630
                     /locus_tag="ACHUOF_000172"
                     /pseudo
     CDS             70226..70630
                     /locus_tag="ACHUOF_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
BASE COUNT        17316 a        19176 c        17559 g        16661 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctacagatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagcag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaagt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat
    63841 ttcagctatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    63901 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    63961 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    64021 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    64081 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    64141 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    64201 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    64261 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt
    64321 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    64381 agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    64441 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
    64501 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    64561 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct
    64621 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    64681 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    64741 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    64801 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
    64861 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa
    64921 attccagacg ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg
    64981 ttctgactcc agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    65041 catccgcgcc atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
    65101 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc
    65161 gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg
    65221 tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat
    65281 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    65341 ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    65401 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    65461 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    65521 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    65581 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    65641 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    65701 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    65761 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    65821 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    65881 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg
    65941 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa
    66001 tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg
    66061 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    66121 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    66181 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    66241 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    66301 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
    66361 cgatgatgta gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt
    66421 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc
    66481 gattagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    66541 ggacgtggtg cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga
    66601 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    66661 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
    66721 ctttgatacc gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    66781 ggaagcgatg aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc
    66841 gcgctatggc actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt
    66901 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    66961 gccgggcgaa ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg
    67021 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
    67081 cgaagactta gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    67141 cgttaagctg gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta
    67201 tgcggacttg atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc
    67261 agtgaaatat gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt
    67321 tgccaacggc ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt
    67381 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    67441 ggtggcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt
    67501 agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    67561 taattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac
    67621 gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    67681 caaacagcag aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa
    67741 gtcactctac tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt
    67801 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
    67861 ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    67921 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
    67981 tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
    68041 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    68101 ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
    68161 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
    68221 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
    68281 tctgggtaaa accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct
    68341 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt
    68401 cgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
    68461 taccggttct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt
    68521 cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag
    68581 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    68641 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat
    68701 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    68761 ctgtctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    68821 aaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca
    68881 gaccaacaca ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    68941 ttcctgcacc ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat
    69001 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
    69061 taaaaaagtg gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
    69121 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
    69181 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
    69241 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    69301 cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg
    69361 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    69421 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
    69481 aggcaaacgc gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc
    69541 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    69601 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
    69661 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
    69721 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
    69781 cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat
    69841 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga
    69901 aggcaacgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    69961 ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat
    70021 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
    70081 gctgaaattc tcctttcttt ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa
    70141 tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac
    70201 gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct caacaggcta
    70261 ccagcaaatc cacaacggca ttattcatct ggtcgatagc gcccgaacgg aaacggtacg
    70321 tagcgttaac gcgctgatga ccgcgacgta ctgggaaatt ggccgacgga ttgtcgaatt
    70381 tgaacaaggt ggtgaggcca gagcagctta tggtgcacag ttgattgatc ggctatcgaa
    70441 ggatttaagt cgacggtata aacgtggggt ctctgcaaaa aacttacgcc aaatgaggct
    70501 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc
    70561 attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt ggcagacgct
    70621 gtctgccaaa ttgagaggct gtttgatatt tccacagctt ttgtattgca tctcctattt
    70681 tcggtttgta tattcatgca aataaacgaa gg
//
LOCUS       DAZOYF010000003        70357 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZOYF010000003 DAZOYF010000000
VERSION     DAZOYF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.003"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..524
                     /gene="intI1"
                     /locus_tag="ACHUOF_000173"
     CDS             <1..524
                     /gene="intI1"
                     /locus_tag="ACHUOF_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR0609523.1"
                     /translation="IIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRS
                     GVALPDALERKYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVE
                     QAGITKPATPHTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVR
                     SPLDALPPLTSER"
     gene            complement(760..1464)
                     /locus_tag="ACHUOF_000174"
     CDS             complement(760..1464)
                     /locus_tag="ACHUOF_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR0609524.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            1518..2054
                     /locus_tag="ACHUOF_000175"
                     /pseudo
     CDS             1518..2054
                     /locus_tag="ACHUOF_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            2266..2892
                     /locus_tag="ACHUOF_000176"
     CDS             2266..2892
                     /locus_tag="ACHUOF_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HFR0609525.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
     gene            2889..3191
                     /locus_tag="ACHUOF_000177"
     CDS             2889..3191
                     /locus_tag="ACHUOF_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609526.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            complement(3710..5056)
                     /gene="yagA"
                     /locus_tag="ACHUOF_000178"
     CDS             complement(3710..5056)
                     /gene="yagA"
                     /locus_tag="ACHUOF_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFR0609527.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKRPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLSNPRIRTNYRECLDAIRQVTLQQEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFKKHMYGVFMHLRY"
     gene            5410..6012
                     /locus_tag="ACHUOF_000179"
     CDS             5410..6012
                     /locus_tag="ACHUOF_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFR0609528.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            6150..6416
                     /locus_tag="ACHUOF_000180"
     CDS             6150..6416
                     /locus_tag="ACHUOF_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609529.1"
                     /translation="MITGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(6607..6888)
                     /locus_tag="ACHUOF_000181"
     CDS             complement(6607..6888)
                     /locus_tag="ACHUOF_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609530.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKSPE"
     gene            complement(6994..7269)
                     /locus_tag="ACHUOF_000182"
     CDS             complement(6994..7269)
                     /locus_tag="ACHUOF_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0609531.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(7269..7553)
                     /locus_tag="ACHUOF_000183"
     CDS             complement(7269..7553)
                     /locus_tag="ACHUOF_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFR0609532.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            complement(8452..9330)
                     /gene="repA"
                     /locus_tag="ACHUOF_000184"
     CDS             complement(8452..9330)
                     /gene="repA"
                     /locus_tag="ACHUOF_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR0609533.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     DSLDISEEAVASARRSRVEWENRLRKKQGMDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASPLV"
     gene            10033..10674
                     /locus_tag="ACHUOF_000185"
     CDS             10033..10674
                     /locus_tag="ACHUOF_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFR0609534.1"
                     /translation="MCLSELQARLNVGQPLFPTHALCIRRSQQHRSPPAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPESHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            10815..11477
                     /locus_tag="ACHUOF_000186"
     CDS             10815..11477
                     /locus_tag="ACHUOF_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFR0609535.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALATC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTGDTFSTTGRMIRPVAFSLIHP
                     ATINTSEVEEIFRDEGFMLAHGRECSLNGSGRNMLSRILHVGHSGLAEEEWGMDNSLL
                     LH"
     gene            11744..12364
                     /locus_tag="ACHUOF_000187"
     CDS             11744..12364
                     /locus_tag="ACHUOF_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR0609536.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            12391..14085
                     /locus_tag="ACHUOF_000188"
     CDS             12391..14085
                     /locus_tag="ACHUOF_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR0609537.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTARVHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            14166..14408
                     /locus_tag="ACHUOF_000189"
     CDS             14166..14408
                     /locus_tag="ACHUOF_000189"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFR0609538.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            14524..14859
                     /locus_tag="ACHUOF_000190"
     CDS             14524..14859
                     /locus_tag="ACHUOF_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609539.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFTIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GHVSITGKK"
     gene            15086..16156
                     /locus_tag="ACHUOF_000191"
     CDS             15086..16156
                     /locus_tag="ACHUOF_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFR0609540.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREAHQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNALLAGQQLYGHMDWNRKILTVTTSATPPVDVTAPQGSQKAADTPRNPFRGSTATP
                     AGPTPVTPAVSVSRAQTTTGTSGTPESRTTTPAPVATKPVVPPQQKVIPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPAE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            16184..16597
                     /gene="pilM"
                     /locus_tag="ACHUOF_000192"
     CDS             16184..16597
                     /gene="pilM"
                     /locus_tag="ACHUOF_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFR0609541.1"
                     /translation="MVIMAIGNFWFHSHLSRTVHHQQTAEITQQAADFIRYMNAINDY
                     LYQHPERRAAGGQLTSAQLGLPATKNVSHLISQQRVFVWAKEKPGLMGALLEQSGDSA
                     LLARVENGRLLDTRGRRISITLPAVIPDQVIIWMN"
     gene            16629..18248
                     /locus_tag="ACHUOF_000193"
     CDS             16629..18248
                     /locus_tag="ACHUOF_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFR0609542.1"
                     /translation="MNKKLLAIMIAATLLQGCTSFRQAENTRKDAVAEQQRAREMIAE
                     LRNRTPVVKESPRQWINPRPVTDKNPGNAVPGCPIIINTRQSITLQQVAQRISQNCHI
                     PVRITPDVQAYLSVTTSGSTQQIQGTVPPPDTGGMVPLAAIGSSDTTLPRLQGNTASL
                     PELKASGLTSLLNAVASRLGISWRYDGSHITFYYLDTRTFPVTYMDSQVAYNATVVSG
                     TMSSNGSSGGTASDAISGDASNTQTTTVDMKSALYQDVKNAVSSMLTPGIGRMFMSTG
                     FITVTDTPQVLETVRTFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDTVTGGVSIVNGKLTGSKAFLKALSSQGDVSVVTRNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            18269..19564
                     /gene="pilO2"
                     /locus_tag="ACHUOF_000194"
     CDS             18269..19564
                     /gene="pilO2"
                     /locus_tag="ACHUOF_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFR0609543.1"
                     /translation="MTDISPTERQVFPLTAGRNVFLAGMDWKTLPASHKNPRTFARSL
                     GAVRYISCEYLSTEDTDRHIMVAAVSQNTIPKGSRRYFSLAMLILPLLEPGGYAIVEL
                     SQANDTELYGFVSAVDGILVSDLVGTREEIREAREIFLTINSAPEHGWTCYEPPSFNG
                     PDGRGPLPLETLTGAGKYPAEARLHPVSRGPQLIPVLLILTLLGTAWYGWQYYERLKA
                     EKAALAEAAQKAAEERITPPWLSMPETGDFIRGCSDVWSRLPVSVAGWRYSLAECAAT
                     GNSGNLRASYTNIAGTTVTAFSQRVREIFSIRPFMVMPDGNSGGFALPVTFSMPETPV
                     AVEALPENTLLQERLTTLARSMQLKMDWQEVSNSFTDEDGNTIQPPWKEYDLQILTTL
                     PAHQVAEHFSEPSVRFISVTRQLEEGRFRYQFTGKYYVQ"
     gene            19554..20012
                     /gene="pilP"
                     /locus_tag="ACHUOF_000195"
     CDS             19554..20012
                     /gene="pilP"
                     /locus_tag="ACHUOF_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFR0609544.1"
                     /translation="MSSKTKALLLSVLILPTTVAASTISNMQQNNRQLQVEQIQEETA
                     LYEAMLAREKVRHELRTLEQGEPAEPVHSQESSSAVTTDTPAPITGIPRLTEISGVHP
                     QITARIQLPGGQTADVKEGQHIPGTTFRVEKITTDSVVLEQNGIRTTLQP"
     gene            20116..21624
                     /locus_tag="ACHUOF_000196"
     CDS             20116..21624
                     /locus_tag="ACHUOF_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFR0609545.1"
                     /translation="MLAPADQPPDDILDYVVVDTDKTGQIHVRVVEGKKHLRAVQDYL
                     TRLCSRYPGKVSPFEFTTLDVIARLRQETVISGVENGINPVQQKMLGYISHAATLSAS
                     DLHITPGRDSTDFTYLEARVHGELELLDIVRKDEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAERFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQHIPTFSMLGYHPEQE
                     RAVRRMLQRPEGIVILSGPTGSGKSTTLRTASAAYLEQYGFNDTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTAQGWVDSVKSALRLDPDAILNGEIRDHASAITAIKAAMTG
                     HLMLTTLHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQVICPHCRLPWHEV
                     ESSRTDEERRLVENFCQPDAVYLRNHNGCPHCWRGVNGRTVIAEVISPDAKFFQIYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
     gene            21626..22720
                     /locus_tag="ACHUOF_000197"
     CDS             21626..22720
                     /locus_tag="ACHUOF_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFR0609546.1"
                     /translation="MKILERLNNTELELSGLNRWLGKKTFRRTTFYESLAGMIRDGTP
                     VMRALEFICDVETDFGKKKGQSGLYFLATDCIASIRSSGQLSPALKDWVPKDEIALIR
                     NGEERGDIAEAMFQVVKTAKGRQEMISSLVSVCLYPLILLTLCVVNMYNVHTRVIPMI
                     SAFAPEERWSVQMVILAKSSGLVIGYGLWLLAGLLLLAILIRMSFSLYTGPRRRYLDR
                     IPPWSLYQTFHGVNYLFNISSMLQINIPLSHALTRIEKHAEHNRWLKSRISAIRKNVL
                     SGQSLALAMKNCGYDFPSKSCVNNLLLNSEREDSVASMALYADRWLEEAKKNVRKSGI
                     IITAISGLLVFLFIINMVSAIYGISDMLKQ"
     gene            22782..23318
                     /locus_tag="ACHUOF_000198"
     CDS             22782..23318
                     /locus_tag="ACHUOF_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFR0609547.1"
                     /translation="MSVITSSYSEHNIRHLQNDDKGMTLLEVLGVLVVAAIVIGAVMG
                     LMSDTLSSSDNQKELKNLQTIATKMKAQKFQGQYTGTDYVKILTESGGLPADMIAGGN
                     KAKNAWGGAVTIKVSSDKYSYVIESSNVPKKNCIDLVTSLRSSSMFTKINGNVTNKVD
                     PSTVCNADKTTIKLETNS"
     gene            23363..23848
                     /locus_tag="ACHUOF_000199"
     CDS             23363..23848
                     /locus_tag="ACHUOF_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR0609548.1"
                     /translation="MNRLFILTVIFSLLCSLPSAQASPQDKQYDRCFITAGKRYSIDP
                     GLLKIIARRESSLNPRAINHNRNDRGKIISTDYGIMQINSTHIPGLKKMGIIRSEKDL
                     LNNVCLNIQTGAWILARHFQRCGVNWECLGSYNAGFSKSNTHRRMKYARQIYSAYMQG
                     R"
     gene            23864..24490
                     /locus_tag="ACHUOF_000200"
     CDS             23864..24490
                     /locus_tag="ACHUOF_000200"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR0609549.1"
                     /translation="MLQIIPALLVAITVVIQCLAVQMSRYRLSRMNARPPHHGLALMS
                     GMLAAAAIILRLYQNDSAVGVLFMTIMTGCMTLMTLTDAASCRLPRPFTIMYLISGSA
                     FRLWQGDWLTGLCSAGFWFLLLLLFRQYTSYRRKTETIGLGDVFLIAGIAIWSPPGDI
                     PWIVATAAGGALLYLWCNRQRRITRELPFGPFLCASQYAFTFYPGGLW"
     gene            24508..25869
                     /gene="pilV"
                     /locus_tag="ACHUOF_000201"
     CDS             24508..25869
                     /gene="pilV"
                     /locus_tag="ACHUOF_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFR0609550.1"
                     /translation="MQKDNDKGFALLEILGGLVVISLLMPLFWSYIEDYLNEMRNQSA
                     AFHADAYNTAARTYIADNNARLHSGTLPATFTADELIRKGYLKGLNRSPFGQSYTTGI
                     RRNTSTGRLEALTCSTGGENIKDDALRSIASLLPGLGGFIGKNGTATGVFGGWTDKPG
                     DYGLSCNGGHIAIVMMGDDLQESDRLYRFQVPGRPELNQMNTAINMGGNNLNNAGNVN
                     GQSATLKGDVTSENGWLITKNDKGWKNITYGGGFTMTDSQWIRAVGGKGIITTGEIKG
                     GKVSGGTVRSDGRLSTGEYLQLDKTAVANTKCSPDGLVGRDSKGAILSCQSGVWVGFE
                     SYPVGSPIPWPSATPPQGYLLMNGQSFSCSRYPQLARAYPSCKLPDLRGVFIRGWDNG
                     KGLDGDRNRQLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYIV
                     KAS"
     gene            25983..26177
                     /locus_tag="ACHUOF_000202"
                     /pseudo
     CDS             25983..26177
                     /locus_tag="ACHUOF_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            26342..27163
                     /locus_tag="ACHUOF_000203"
     CDS             26342..27163
                     /locus_tag="ACHUOF_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFR0609551.1"
                     /translation="MKTDSTGIAARMMLSLDRERICECLLSHRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEDALSEMFLPADNIVRRLLSRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGEILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PATQEIFCRIISARENMDIILWHGFPFAGTHSGEYCGVLLRQKKEINNTIIYPVLSLY
                     PARMDIEKEGWFQMARKISPDIPVRAVTLTPAQAEYLITGKILPTALFRVPLSPW"
     gene            27265..28467
                     /locus_tag="ACHUOF_000204"
     CDS             27265..28467
                     /locus_tag="ACHUOF_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFR0609552.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKSGAKDDFSLLLPSVGAQLSDPGNIADNADKISDDWKAFDRAVD
                     SHSGVPQTAARLKERLQDFRNTHASAQAGVSAVAALPGDTLAAALMLKTLGTVSVDGK
                     VSDADQNYLESIADSGSQDVDKNRLTSQAFARAALITDVGVALATELETAGQKWSLGF
                     TPKFQRVDLFNYNTLIKNYDSSAFKGDRYHNTQNGINADIGASMDLDDNWTLGLVAQN
                     LIPRSIETKEVHGVTETFRIRPQVTTGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AVGAEFNAWKWAQLRTGYRQNLASNNGSAFTAGIGLSPFDVIHIDVAGLVGTDNNYGA
                     IAQLQFTF"
     gene            28571..29029
                     /locus_tag="ACHUOF_000205"
     CDS             28571..29029
                     /locus_tag="ACHUOF_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238171.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFR0609553.1"
                     /translation="MQKKRITLAGGVAMLCLSGCHLPPDNTAPARKYGAASEVAVTRH
                     AQYQLWEQGTYSAALPSRQPGGSLQAHSGRVSFDWEGDALELLSELARLRGQQFSYSG
                     VRLPLPVSLHVRDMTFADALRLTEAQTAWRAVLSQSAGRLHLSYMPTEKK"
     gene            29026..29862
                     /locus_tag="ACHUOF_000206"
     CDS             29026..29862
                     /locus_tag="ACHUOF_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFR0609554.1"
                     /translation="MKKQVCLLLSGLLLAGCAGQHHRPPAGMAGNLPPPDLRAWLTPT
                     DNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISR
                     EGWLPPVIDEAQDVAHIQPDQIRTASRVWTILRPERFVSNPPGWRDWLLRGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRDNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQRQTVTGNGRKLITGDHVRRLKTHAEFTLQKSRWRPVVSTEGAPDEI
                     TR"
     gene            29846..30994
                     /gene="traJ"
                     /locus_tag="ACHUOF_000207"
     CDS             29846..30994
                     /gene="traJ"
                     /locus_tag="ACHUOF_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFR0609555.1"
                     /translation="MKLPDEFGTFPFARFTAEEFRYFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSAHPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKVISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRYIQTHFPDRKTVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRDTLSRQS
                     HATWGHYIDEIIRKEKRRIRDQVLTMYQTGSIEASEAALFIPAGEFPK"
     gene            30991..31281
                     /gene="icmT"
                     /locus_tag="ACHUOF_000208"
     CDS             30991..31281
                     /gene="icmT"
                     /locus_tag="ACHUOF_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IcmT/TraK family protein"
                     /protein_id="HFR0609556.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFVLLGYSGWTVRTLCRRLYSLVQGKQASGRPWWYRHRTELPREWRGTE"
     gene            31345..35406
                     /locus_tag="ACHUOF_000209"
     CDS             31345..35406
                     /locus_tag="ACHUOF_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFR0609557.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYRAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQAGYARSYINRLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDASNMIAVAENARQIWTDSPFVFCADNDHQREINKGVFSATKAAEVTN
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDNFQNAMNAQLKHIGIPTPNSDTADHREA
                     VVIDNLIFTPVKDEKPQMSPENRQSTAPETELATQDTPYDTHSDDTENQAYEDDYAAY
                     ASLQSVTAETLSPDKPLENIASAADTGTENTSVPTAGDQEDLNTDATGTDTAHNSDTA
                     ETGTLPEPAGMQSVQQATTEPTAEDKPHENVVSAADTREENASVATADTGEENASATT
                     ADNQSTQAEEHPRSKDTPETSLSDAAGTQHTTTTQSEEQVQVSMASADDWSAFAEDLN
                     ADTPGAPTVHNTDNAETNTVSEPAGMRPVQQVTTELTAEDKAQEKSVASAAETGAEHS
                     SDITDDNRNAPAEELNADATVAVHNTGTTETASPSEPAETQPVRQATAEPTAEDKLQE
                     ERLVSATETSTANEAARPETPSLFSRLRRFLTGVKSRTSEPSVIQEKKPTEAETATEE
                     VAPPSPPETSPEDTSSAEPDTIIFAPRRPDSPQRHNLDEILKNLTHKEFPDRTALYSV
                     DGEPAFLDRLYCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIV
                     EYDIKVRMKLPAQRAALEKIRKEKEMAHDAIVTHRPTPELNRQSPEETTMAQPAPPVS
                     PKAEAEQTAGSTAETPHPEAPVTAKAEAPRADRPEQPAAPSSAPEKAPVSAQPQQPDT
                     PEEEPPGKLSPGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVK
                     NHQKGDPVTLTLQARETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHTLEFP
                     MATFSQVMLQMQQHWPQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAP
                     NELIPAIASADKDNRQLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINAL
                     TDNGLQFAGYGEAVNHDENNHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSL
                     GFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPGR"
     gene            35423..35773
                     /locus_tag="ACHUOF_000210"
     CDS             35423..35773
                     /locus_tag="ACHUOF_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR0609558.1"
                     /translation="MNMKKTLLVILTGITLASPVAGAATCRAGAAAEAGSRAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDISITLPQFPSLDKLLRDLENQVCDAVVDRVND
                     HLPGNIDPWKDYNL"
     gene            35785..36480
                     /locus_tag="ACHUOF_000211"
     CDS             35785..36480
                     /locus_tag="ACHUOF_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFR0609559.1"
                     /translation="MSEAQPTSPVSPEADNSGDIYNPVLRTIAQSEQRAALVPGLVRA
                     LLYSGAALCISLVANGIQYWHATGVTREYFATDNGRLVRLAPTSQPAWSQSDVMNFGS
                     MTLSEAFNLDFVHYRKQITTQAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTTTIG
                     AGTLVNQGQMENGVWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAAAGI"
     gene            36491..37465
                     /locus_tag="ACHUOF_000212"
     CDS             36491..37465
                     /locus_tag="ACHUOF_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFR0609560.1"
                     /translation="MHIHHLIGALLIVCGTVTAADNPGWKNARSPQTGGKTAQATGNT
                     PAGGPPPAAQLPGNLPPPGAALVQEGASLDSPLNASEIRELRGIMADNERAISAPLTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDVNGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTTTLSQEIDSRLDLRIPR
                     RGPASPVVSIPSEKIALHDATLQAFLDGVPPDDPAIRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            37469..38806
                     /gene="traO"
                     /locus_tag="ACHUOF_000213"
     CDS             37469..38806
                     /gene="traO"
                     /locus_tag="ACHUOF_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFR0609561.1"
                     /translation="MSAEQEQDTAAKSGKKLSALLVMGGVIIIGGGYLLLTWLSPENA
                     ASLSSVNINSTTSGGGRNVMETQHYRDLLRADNERGAAEALRNNETFIASLPRGLDTD
                     TGNDQKRDNPPDDQSGTSGQNASPPQEDQTAREKRREALQKLLARINTQHQDARPPVV
                     AAAMWSTTGTPGTAPHASPSGTVTSQHPRLQETSLATPATRQGIQLIPALTRVPAYID
                     TEVDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGQGMDIHFDRMMWRGMFLKVNAYA
                     LNERTGMSTVASDVNHRWFKHIVLPSVLGGVGNVGSLYKDANTQILQGNYGSITGRVG
                     MPDGEVLAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDIST
                     PSSGTAPSESPSSGRPLTQAEAEHQASDRIRAAVLRKQNEMQQRYGIQQESYP"
     gene            38803..39516
                     /gene="traP"
                     /locus_tag="ACHUOF_000214"
     CDS             38803..39516
                     /gene="traP"
                     /locus_tag="ACHUOF_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFR0609562.1"
                     /translation="MKNEHEDPGSFGETTAPASGKSFLKRQYMGYSLPVWAGCALIIL
                     VAIWYKFLRTPSEPPADAGFAVTPPAAESWAEPMTMTESGIGNNMPAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKDSITTLSNAIQKDEAWAQETRQLLEETRKQVHTL
                     QMQGALPAPTGNNRQPAKTLPSPLQGMKIMSVEKGMAWIRWQGSAWAVREGDSLGNIT
                     ITRIDPSSRAVITSAGTLR"
     gene            39513..40043
                     /gene="traQ"
                     /locus_tag="ACHUOF_000215"
     CDS             39513..40043
                     /gene="traQ"
                     /locus_tag="ACHUOF_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFR0609563.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIFICGMLVSLPSMINAGGTQMGFRADSFAPIAYVQPGSFGAA
                     AGAVNAILSLAKLAGVGFVMNGISIWRKSLLDGHTALSASESISKGNVKFVAGVLLVF
                     NDRVIDATLASLGIAF"
     gene            40090..40488
                     /locus_tag="ACHUOF_000216"
     CDS             40090..40488
                     /locus_tag="ACHUOF_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFR0609564.1"
                     /translation="MSRLRAMAARLRAASVYVQLCLMACRDRLVRLLLLLPVMLLPHT
                     AMANTLFDMIKTVGDGARGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGVCLAVVPELMGSGQKTLGLSPVSIS"
     gene            40545..40796
                     /locus_tag="ACHUOF_000217"
     CDS             40545..40796
                     /locus_tag="ACHUOF_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFR0609565.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKAYEKDPATHRPGPGTMAFPAPATGHGIQR"
     gene            40711..41529
                     /locus_tag="ACHUOF_000218"
     CDS             40711..41529
                     /locus_tag="ACHUOF_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFR0609566.1"
                     /translation="MKKTPPLIAPVRERWLFPLPQPDTVFSGEALLPPPVLSTEGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTIVLLEKNLTRVMRLKKRPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESDLLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALQEIFSDNRQRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            41824..44868
                     /locus_tag="ACHUOF_000219"
     CDS             41824..44868
                     /locus_tag="ACHUOF_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0609567.1"
                     /translation="MNINRIIFAIEDCISTLSRYTVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDQTAPGTLESLIAALAERMNTAW
                     KNSGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKNHQTRVRKLAEHAPPARFAQDPWRWTLSALKIRHDALLD
                     TLEQALTHDSDGLLIRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGGD
                     TSVFHAPSLNLQLFTTQPETHGSLIQAGELWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPDGMKALGVKKTLLTYSSFIPPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHSSGPIPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSESQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PDHTRNLFDVMYGARKPVTPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISSLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHIAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSEERLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQHYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQEVGSLRARQI
                     LAEHFPRGSATSLIEHRARTHDSENVIHELAAELIRKQGYNL"
     gene            44868..45488
                     /locus_tag="ACHUOF_000220"
     CDS             44868..45488
                     /locus_tag="ACHUOF_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFR0609568.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     APLTALMSDNTWLWPDVPGTPALTAGDWPVMESDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPKSLSHRVPDILYPPKTEKKECKDNLQPHCLHSL"
     gene            45446..46651
                     /gene="traW"
                     /locus_tag="ACHUOF_000221"
     CDS             45446..46651
                     /gene="traW"
                     /locus_tag="ACHUOF_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFR0609569.1"
                     /translation="MQRQSATTLLALLVGCAVIPATHAYSVNVVSSQPVTRQIMPELA
                     TIQGIMEEMVRMQTATGTAITQNSEKLATVIAQDGQATRQQMIFSNETHRLEEARKSF
                     SVPDSICSESASGIAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPVREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGDLPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            46648..47217
                     /gene="traX"
                     /locus_tag="ACHUOF_000222"
     CDS             46648..47217
                     /gene="traX"
                     /locus_tag="ACHUOF_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFR0609570.1"
                     /translation="MKDDNSIDEKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     DQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVDELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPVITLIRILVCLLLMSTIAALCASKALIASYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            47292..49451
                     /locus_tag="ACHUOF_000223"
     CDS             47292..49451
                     /locus_tag="ACHUOF_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFR0609571.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIATMGIVWFLFIGIRHAVRTGHQGSVFNN
                     GRDVVGILSVVSGFLMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMELCKHAMNTGLNDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLSVKGNGSNKQSALAHFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPADNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLEDLANRAPTVSGVSSLGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTNTSSTEYRTAEAAEPKSIIMKVWSGENFADKIATSLSGTGTSSD
                     QLNPLIKMKNIGDYTMGITESIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAKAF
                     LEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWAAT
                     HLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFGAALVNV
                     QADSLTGLFSIAGFLLIYARVCTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNMVS
                     SVKDIIAGGNTNMRRTPGVREEKLKNVNGDDKDGIKQ"
     gene            49539..50186
                     /gene="excA"
                     /locus_tag="ACHUOF_000224"
     CDS             49539..50186
                     /gene="excA"
                     /locus_tag="ACHUOF_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFR0609572.1"
                     /translation="MSTTKSRYPSWWPYYYLVRNAYFIFGIPCLLFYAFFGTLIVHDS
                     ALSQTMDFIFAYGCWALIILPLLWLYLRTRAKKKKVQQVVQQIKESCHFAPTSDYEQL
                     SFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSLP
                     MIPLEISGISSRTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            50460..52046
                     /locus_tag="ACHUOF_000225"
     CDS             50460..52046
                     /locus_tag="ACHUOF_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR0609573.1"
                     /translation="MSESRSNFPARHGTPWNTEELQRLLVSIAEGVSLKKLTQTHQRS
                     SGGIRGAIKRLLPSEFLEFNDINTVNDLSNYFNKIPRQEHNSIINNLISLSTAKTIAH
                     DKVEIQKNSFKQNTPLPLCQPNTDFKDYIADEHDVIMLIHSAIESLSNKRHQYILLMR
                     LGTDDSPHSLADIAVKLGISRERVRQLQERALGLLAAKSRYEGTPGACLKALINSIGN
                     SPYDIAVWIYRIVHRDFVTSSELAAKFIIFAAGIPKARRDEIKNSLSLISQLQKQKLR
                     ERRAELAHAHAQERARIKKERVEFIFSKWLDNTHWPKYIAPPPPVEQLCSQRAVNDTD
                     ISGFFYSQKLGRTVQYESGLEFKIMNLLECCDQVLFYQEQPFSIPYCFKNKSKKYYPD
                     LLIATVDGRCLLMEVKPTDRMTLSLTRIKSNAGRKWAHTRGWGWLVISDRFSLRQLEE
                     HAISTNTWKLIEAELKTSRILAWREMMELRTRYEIHRLDLIAYIIQSGAEVDNFYRIT
                     PKISNNTSNQRQPNESDCHN"
     gene            complement(52184..52336)
                     /locus_tag="ACHUOF_000226"
     CDS             complement(52184..52336)
                     /locus_tag="ACHUOF_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0609574.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRIEQGNTVFVATL
                     AYEVKR"
     gene            52666..52908
                     /locus_tag="ACHUOF_000227"
     CDS             52666..52908
                     /locus_tag="ACHUOF_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609575.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALTMLSTLCYNLM
                     MTCGIHSMDSQMLNGHGISMQLQGIDIAEKPGKNIH"
     gene            53199..53567
                     /locus_tag="ACHUOF_000228"
     CDS             53199..53567
                     /locus_tag="ACHUOF_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609576.1"
                     /translation="MKDNNTTATPETWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIQPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILSRYPHKKDEV"
     gene            53571..53786
                     /locus_tag="ACHUOF_000229"
     CDS             53571..53786
                     /locus_tag="ACHUOF_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609577.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(53984..54607)
                     /locus_tag="ACHUOF_000230"
     CDS             complement(53984..54607)
                     /locus_tag="ACHUOF_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0609578.1"
                     /translation="MQEHIDYQIEKYQFRARNESPRLMRQWEDVMRECRDTGAGARER
                     LRIALLNVDYVTSFELPFRLLLTRTPQMIDSVRREWQLSQKKVVFNERQRGCVYSLQN
                     DLSGVPDSFSYSLATRIRRADSYGMTTRCFTDIAGQVKAPTARLKLALESGLIVTALD
                     GLFWLGIQRIAADVQRLRQSGMTIITRDVEVSDSLTGTTRVVSGYSL"
     gene            54767..55234
                     /locus_tag="ACHUOF_000231"
     CDS             54767..55234
                     /locus_tag="ACHUOF_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR0609579.1"
                     /translation="MIKYIFSLTIIFSASLCAAQIQGHVIRVLDGDTLEILQDKKPVR
                     VRLANIDAPEKKQPFGRWSATQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEAN
                     RWQVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
     gene            55384..55560
                     /locus_tag="ACHUOF_000232"
     CDS             55384..55560
                     /locus_tag="ACHUOF_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609580.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYISWGVPGKSRLMFDSR
                     EQAQEVQEGFRFLT"
     gene            complement(55623..55718)
                     /gene="dqlB"
                     /locus_tag="ACHUOF_000233"
     CDS             complement(55623..55718)
                     /gene="dqlB"
                     /locus_tag="ACHUOF_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFR0609581.1"
                     /translation="MINLLIIVLRAVVAVANAVIAILELIRELVD"
     gene            complement(56307..56402)
                     /gene="dqlB"
                     /locus_tag="ACHUOF_000234"
     CDS             complement(56307..56402)
                     /gene="dqlB"
                     /locus_tag="ACHUOF_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFR0609582.1"
                     /translation="MINLLIIVLRAVVAVANAVIAILELIRELVD"
     gene            56705..58012
                     /gene="trbA"
                     /locus_tag="ACHUOF_000235"
     CDS             56705..58012
                     /gene="trbA"
                     /locus_tag="ACHUOF_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFR0609583.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFGQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEVFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTGETTETTETTETTETTETTEKQHPADKEKEQ"
     gene            58009..59133
                     /locus_tag="ACHUOF_000236"
     CDS             58009..59133
                     /locus_tag="ACHUOF_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFR0609584.1"
                     /translation="MTIHYTATLNNKHLSLIALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            59114..61417
                     /gene="trbC"
                     /locus_tag="ACHUOF_000237"
     CDS             59114..61417
                     /gene="trbC"
                     /locus_tag="ACHUOF_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFR0609585.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            62281..63096
                     /gene="sul2"
                     /locus_tag="ACHUOF_000238"
     CDS             62281..63096
                     /gene="sul2"
                     /locus_tag="ACHUOF_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR0609586.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            63157..63960
                     /locus_tag="ACHUOF_000239"
                     /note="aph(3'')-Ib"
     CDS             63157..63960
                     /locus_tag="ACHUOF_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFR0609587.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            63960..64796
                     /locus_tag="ACHUOF_000240"
     CDS             63960..64796
                     /locus_tag="ACHUOF_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR0609588.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(64903..65307)
                     /locus_tag="ACHUOF_000241"
                     /pseudo
     CDS             complement(64903..65307)
                     /locus_tag="ACHUOF_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(65657..66088)
                     /locus_tag="ACHUOF_000242"
     CDS             complement(65657..66088)
                     /locus_tag="ACHUOF_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFR0609589.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(66156..68867)
                     /locus_tag="ACHUOF_000243"
     CDS             complement(66156..68867)
                     /locus_tag="ACHUOF_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFR0609590.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGSGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(68879..69211)
                     /gene="mobA"
                     /locus_tag="ACHUOF_000244"
     CDS             complement(68879..69211)
                     /gene="mobA"
                     /locus_tag="ACHUOF_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFR0609591.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            69444..69779
                     /locus_tag="ACHUOF_000245"
     CDS             69444..69779
                     /locus_tag="ACHUOF_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFR0609592.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     ILVRTKGYTLTDVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(69865..>70357)
                     /locus_tag="ACHUOF_000246"
     CDS             complement(69865..>70357)
                     /locus_tag="ACHUOF_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFR0609593.1"
                     /translation="LAYDFFRILRAPLLATFRHLNASKMQTFEQGLIDVYRKLSWDYR
                     TNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRNSIG
                     AQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPGALP
                     PRV"
BASE COUNT        17589 a        19329 c        17584 g        15855 t
ORIGIN      
        1 cgatcatcgt gcgggagggc aagggctcca aggatcgggc cttgatgtta cccgagagct
       61 tggcacccag cctgcgcgag cagctgtcgc gtgcacgggc atggtggctg aaggaccagg
      121 ccgagggccg cagcggcgtt gcgcttcccg acgcccttga gcggaagtat ccgcgcgccg
      181 ggcattcctg gccgtggttc tgggtttttg cgcagcacac gcattcgacc gatccacgga
      241 gcggtgtcgt gcgtcgccat cacatgtatg accagacctt tcagcgcgcc ttcaaacgtg
      301 ccgtagaaca agcaggcatc acgaagcccg ccacaccgca caccctccgc cactcgttcg
      361 cgacggcctt gctccgcagc ggttacgaca ttcgaaccgt gcaggatctg ctcggccatt
      421 ccgacgtctc tacgacgatg atttacacgc atgtgctgaa agttggcggt gccggagtgc
      481 gctcaccgct tgatgcgctg ccgcccctca ctagtgagag gtagggcagc gcaagtcaat
      541 cctggcggat tcactacccc tgcgcgaagg ccatcggtgc cgcatcgaac ggccggttgc
      601 ggaaagtcct ccctgcgtcc gctgatggcc ggcagcagcc cgtcgttgcc tgatggatcc
      661 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagggc actgttgcaa
      721 agttagcgat gaggcagcct tttgtcttat tcaaaggcct tacatttcaa aaactctgct
      781 taccaggcgc atttcgccca ggggatcacc ataataaaat gctgaggcct ggcctttgcg
      841 tagtgcacgc atcacctcaa tacctttgat ggtggcgtaa gccgtcttca tggatttaaa
      901 tcccagcgtg gcgccgatta tccgtttcag tttgccatga tcgcattcaa tcacgttgtt
      961 ccggtactta atctgtcggt gttcaacgtc agacgggcac cggccttcgc gtttgagcag
     1021 agcaagcgcg cgaccatagg cgggcgcttt atccgtgttg atgaatcgcg ggatctgcca
     1081 cttcttcacg ttgttgagga ttttacccag aaaccggtat gcagctttgc tgttacgacg
     1141 ggaggagaga taaaaatcga cagtgcggcc ccggctgtcg acggcccggt acagatacgc
     1201 ccagcggcca ttgaccttca cgtaggtttc atccatgtgc cacgggcaaa gatcggaagg
     1261 gttacgccag taccagcgca gccgtttttc catttcaggc gcataacgct gaacccagcg
     1321 gtaaatcgtg gagtgatcga cattcactcc gcgttcagcc agcatctcct gcagctcacg
     1381 gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa
     1441 atgccggcct ttgaatgggt tcatgtgcag ctccatcagc aaaaggggat gataagttta
     1501 tcaccaccga ctatttgcaa cagtgcctct gtagtcgcag ttttggtgaa cgaatcacag
     1561 acaaagatgc catgcaccag gctgttgttc agtatgcaga gcgggccgca gagaaactac
     1621 gtggggagcg tcagtattgc cggcaggtga cgacatttgt acggacatcc ccctttgcag
     1681 taaaagaacc ctgttacagc aatgccgctg tggaaaagct tccattgccc acacaggaca
     1741 gccgggacat tattgccgca gcatgcagag cgttaaacca tgtctggcgt gaagggtacc
     1801 gctatatgaa ggcaggtgtc atgctggctg atttcacacc atcgggtata gcgcagccgg
     1861 gattatttga tgaaatccag ccccgtaaaa acagtgaaaa gttaatgaaa acactcgatg
     1921 aactgaacca gtcgggaaaa gggaaagtgt ggtttgcggg gcgaggaacc gcccctgaat
     1981 ggcaaatgaa acgggaaatg ctttctcctg cgtataccac cagatggaca gatttgccag
     2041 tagcccagtt atgaatatgt aagccaccca taatattgtt atcctgttat cttgttatct
     2101 tgttatcttg ttatcttgtt atcttgttat cttgttatct taatcaatct ataaatcaaa
     2161 tacttaacaa cttgctaagt tgttaacata acaaaaataa ttgcatggtt cgcgttcaga
     2221 tggcaatatt aagttgttaa cttaacagct taatatgagg ctataatgat aatagtatta
     2281 ggtagtcaaa agggtggggt aggtaaaagt acactggcgg tatctatagc tgcgtatctg
     2341 atgtcactag gcaatagagt gattattgtc gacgcagatg accagaaatc agtattaacc
     2401 tggtacaaca atcgccctga agatctccct catattccgg ttacaggcgc gacaggtaac
     2461 atcaaagcca tgttaaagga acacgaaaaa tcctatgatt ttgttattgc agactgtgct
     2521 ggccgtgata gcgcagaaat gcgtagtgga ttgatggccg cagatgtatt catttctcct
     2581 ttgcgcccat ctcagatgga cctggatgtt gtgcctcata catgctccgt ttttacagca
     2641 gcaaaagatt ttaatgagga tgttcgggga tatctcgttt taaacatgac gcccacaaat
     2701 atgtttgtta atgaagccaa tgaggcagct gaagtactta aggattaccc tgaaatgcat
     2761 ctggcaaact cccgggtatg tgatcgtaaa gcacatcgag atgcttgggc cgaatcaatg
     2821 acaatttttg aaacgcaaaa tgataaagcc cagcaagaaa tcgaagcgtt agtgaaagag
     2881 gttattctgt gagaaataga gcaaacactt cacctcgtga cgaggcagca tttattaacg
     2941 gtggcacggc tggccttgat aataccaaaa agtcatcaga taaatcatcg gttgtaagca
     3001 aagcaaaacc agtgagtatt tctttttttg aagaaaacct aaaagacatt gatgccctta
     3061 taacggctga gatggtttca ggtaaaggca gagtgaatcg ttctgatgtt gtcagggcgg
     3121 cggtagaagc acttaaaaca ctatcaaaaa cagaggtatc acgattgata gcagaagtaa
     3181 aacaaagata acaccttgag ataacaagat aacaccttgt tatcttgtta tcttgttatc
     3241 ttgttatctt gttatcttgt tatcttcaat gtattttagg atgacctatg ttgaatagaa
     3301 cctaaaacaa aacaaatatt aataggggtc cgcttggaaa agggaaattt tcccacgcta
     3361 agagaattga gttgaacgag tggttacatc caggccagct aactcactga accccgtaca
     3421 gggtcagaca ttcggtatct ccttcatcct ctgaggaaaa caaatgaatt aactgcttac
     3481 gcatatggat acatatacag tattaattta ttaaggagaa aatggcatgt caagatttga
     3541 tgatagtgat tatcattaac atgaagtgat gttacaccgt tcagaaagcc tgctgacgct
     3601 gtggcagcat cacagtctgg gtattctctg gtgcaataca aaaaccggac tttcccgtaa
     3661 caacagacaa tgttacggga aaagttacag aactgagcca caaaatatct cagtacctga
     3721 gatgcataaa aacaccgtac atatgtttct taaaaggggg atgatcgtta tccagccatt
     3781 tcggtggatc ggtatgcaga acaccaatct gcttcatgcc ggtatgctgc ctggattttc
     3841 tcgcgcagta atcactgtct tcatatccca tcgtttcgat gaccactctg gccgtctttc
     3901 cgtcaggagc ccttgctgtg acaatgaaat ccggcagtac atatttcttt tcaccattaa
     3961 cataaacttc tgtatcaaac aggggccttt ccagacttaa ttccggcgta cctggctttc
     4021 ttttaagcca gctggccaca ttcagaagag cagtgagcgt gtttcgttcc agtttgctgt
     4081 ccacaggcac cggacatgtc agttgataaa gagcatgagc atacccttca ctgcagatga
     4141 ttctgccatc ggcactccgg caaaaagcaa gaataaccca gtaaggggga cgtattccgc
     4201 cctgcgcttc accattaata ctgatccccc gcacaggtct gatgacctga atattaccgt
     4261 tcgcccagtg aatttccaca gcatcacgac agatgtgctg cgccataaaa atctgaaaga
     4321 acatatgctt tcttctggca ggccagtgtc gttctgaatt ctccagcgtt tccattaacc
     4381 gctcctgaga cctttcagac attcctggcc ggaaatggat aatttcagat aaggctcttc
     4441 cccgaatata ctcctgctgt agtgtaacct gccgtatggc atcaagacac tcacgataat
     4501 tagtccggat acggggatta gacagggaat ccagtttatt cagccctgca tcttcaataa
     4561 gcgacaggag taaacggcca aggcgcggac gtttcttgcg aacggaggag cgctcaccat
     4621 tatggtaagc agagcgggcc tgcagtctga taacagaatt actgtcatca ggtggtaaaa
     4681 agtttctgta atttatacgg gtgctaacgg gatgccggct tgctccgaag gatgtttcct
     4741 cttcatttcc ggataactga cgatacatgg ggcattcagg tgcatgctca tgattaaaac
     4801 tggacagata aagagtaccc gtattgtttt taagagcagc aaaattcatg gccggcgaat
     4861 ctcccggaat acagtcacac tgtaaccatg catcatactc acgcatttcc cggagaagat
     4921 cattggccag gttatgttgt gccatggttg ctggcctttt cagtccacag accgtcttta
     4981 ctgcagtctc ttcctcactg ctaagctccc gaatcacttt gccccgggga ttcttcttta
     5041 caagtatcat tggcataatg aaagcactcc ttatgtacat gcttaaacag taagtaaaat
     5101 atacaaagaa caccccatga aacaaaggca gagatgaact gtgcgccccg atatcttctt
     5161 ctgatatcct ttcaaatgaa ttgtttttat ttatatcatg acaacattaa agtgagagaa
     5221 tatatcaacc atgtttctgt cagggttcag tagaatatca attcgactgt cacgccataa
     5281 ctgtaactac ctcgagtata aatattcaga gaatggactt attagccatg aaaaatcacc
     5341 tgatgggaaa tttactaatt tatggattgt gatattatta aagagtaaaa ccagccagcc
     5401 gaggtattta tgggaaaaaa tcaggaagaa aggaaaactc ccgtcattgt ggtgaaaaaa
     5461 cgtcgtactt tttcgccacc gtccttatcc gaaaaaacag atattatcgc acctgtattt
     5521 actgaacaga cggcagaatc agcccccgcc ggaataaatt catctgccgt cgaaacacat
     5581 atccccgaag cgccagccag gaagaagaaa aagaaaagac accgttttcc acgtccctcc
     5641 cactggaccc gtgagtatac tcacgagtgc gtggaaaaaa taaaggccct gtttcctcat
     5701 cttcgtgctg aaggtggtgg atttattccc ctgaaaattg gcataaacaa tgatatttcg
     5761 gcatttctgg cagaacaccc ggagacagaa ctgaccatgg acgaatggct ctgtgcagtg
     5821 tcctgcatca catcccgccg cgtatatctg cagcggacag ctgtcgccgg cgtcccacgt
     5881 tatggcctgg atggtcaccc caaagggcag gtctcagact ctgaagcaca gagtgccggt
     5941 cgcagactgg cgacattaga acaaaagtgg ctcaggatgc aggcacagca ggagaatatt
     6001 tctgggcaat aaacactcct gataacaggt ggacaaaaaa aggaagcact tgataacatc
     6061 aggaatttta ttgataaaca gcagttaacg aatagtgata ctccgaagaa aagcacggtc
     6121 ccaaatgaag ataaaaaagg cataagatga tgatcacagg aaatggaata aacactgtaa
     6181 ctgtcaatgg aaaagtgaaa cacatcacag aactggatga tataacactt tgtttagagt
     6241 ggacaaaact acgagaggaa aacaatcgtc tttatgaaat taataaccag gcaaacagag
     6301 gatggcgtgg tttcatccta agattgatag gggttaacct gccagataaa aggactgagt
     6361 ttacacaacg tctcttattg acgcgcaaaa tttcgggttc ggtcatgaaa aagtaacata
     6421 ttctatgctg gaacagacca attttgcatt cgccgtccag gagtttgagg ggaaagtagt
     6481 cagtgtttac gctcagtcag ggaaagtgtg caatgataaa caacacgaaa ggaatcgtag
     6541 tacatgggca ccgcgattca atattcaagg aaaacacaat acagcaatgt acttgtactg
     6601 tttctattat tctggcgatt tcaataaagg cacttgctcg cgctctgtca ttttctgaaa
     6661 ctcttcatgc tgcatttcga ttttccttct aagatcataa ctcgcatacc tgttcttttt
     6721 atgtatatct atatctattt ttgtgacgag ttcaaaccac aatgttcgca ctccccaggc
     6781 agcgttatga aacatttccg caacggctat agagtctgta ttaactgctt cggtcatgta
     6841 gtcgctaata cgttcagcgg taattttcgt tatttcatcg taattcattt tatctcctac
     6901 aagattattt aatggaatga aaaatatctg gcctgataat aatgttccag aattaatatc
     6961 tgcaggctat gttaagttgt ccccacacac aatctacctg ttttctcgtg tgtgccacag
     7021 gcgcaataca taaatagtct ggccattaag ttcgtaacgg atttcgtact cgccagcaaa
     7081 aatccgtctg acctccctgg gttcaaactg aaacaactgt tctcccatac gtggatgagt
     7141 taacagaatg accggtgcct gtgtcaggga ttgcaccgtt ctggcggccg caggtttact
     7201 ggccagcacc agaaaatcat ataaccgcgc caaatcagaa agcgccttac tggtccactt
     7261 cagttccatc agcgtggcac cggtaacgga ttgtcagtac tgaggctgtc cgcccaggcc
     7321 tgtacagcct gatggtcgat aacctgtccg gatgtcacat cgtccagggc ttccagcgtc
     7381 aggcgattac gctcctcctc ctgagcaagc catgcagaaa gcgcctgttt gataatccag
     7441 ccccgggagc gttccagtct ggcggccatc tggtcgactt tatcagccat aggtaatggc
     7501 acatgcgcgg taattacttt tgttgcctgt gcggtattgt ttttcatttg catatccgat
     7561 tagctgttaa tcatatttaa tcatagtgat tactttcaga cgaaacaagg caaataatgc
     7621 gcgtcagcgc atgtaaggcg gcactccgtt gttacatatg gcacagaaac aaaaccgccg
     7681 aagcccggcg tcagccggta ctgattgata gatttcacct tacccatccc cagccctgct
     7741 aagccatacc cgttttcagc catgacagag cttctgtgcg cggtcggagt ggtcccgacg
     7801 agggtttacc cgaagtcggg gcgtatctcc gcgttagcgg gccgtcaggg ccgcttacga
     7861 gcgtgtactg aggacttcca gccagaagac tgacagcgat gaggattctt agttacaaca
     7921 ttcataatta aaagcgatgt tgttcccgcc cttcgggcgg gggcggcctc gcttttcagt
     7981 tatgaggggt aggcacggtg ctgcgtttgt gtggtgtttt tattcctgat gtctttttgt
     8041 tgtggtttca gaacggtgag gttcggggcc cgcgcgtcag cgtgggaggt gcatatttgt
     8101 ctgtccacag gacaggcact gaataggtct tttttttaaa tggaagtagt taagtagttt
     8161 aaaggaaata cagtactggg gtttaaaaga aacattgcac cctgtaacgc ctgcggctgg
     8221 cgctttatag cattaacggt tgtaacctta cggagaagtc ccttgcagtt aaatgtggat
     8281 aaacagaatt ccccgtctgt gagacgtgtt ttgcgtgaaa aacaggggga gcaaggcgag
     8341 ccggaaggca gatgatgagg ctgttttttg tgtgctgtgc tgcttttttg tgcgtcgccg
     8401 ggcttcagac gtgcggaagc ctccggcgca ggccggatta ttcagatgag gtcaaactaa
     8461 gggtgaggca gtggccagcc gtgtgtagtt gttgttccgc gacattatca tgcgttcctt
     8521 cacgcgacgg tcgatttcgc gcttcacggc ctccaggctt ccccggaaac gcccttccgc
     8581 gatttcacgt gtcagctgcc gtttcaccag ggtgacaatg tcctgacggg tcctgcctgc
     8641 atcgcggcgc gcacgggcac gtttcatgcc gtgggactta agctctgcct gataactgcg
     8701 gaaacgctcg cgcacaaaac gccaggccct cgctatcagt tcatccatac ccagcgcatc
     8761 catcccctgc tttttgcgca gcctgttttc ccattccacg cggctgcggc gtgcggaagc
     8821 cactgcctct tctgagatat cgagtgagtc aaacagtgcc ggcgtgaacg tgatgtcggt
     8881 cgggatgtta cagccaattg tcgggtcgta ttccgtctgg taggttatca gccccagctc
     8941 agacaggaac ttcagggcac gggtggcacg ggtgatggag agtttcccgg cctcagactc
     9001 cgtcgccagt ccgcactcaa tggccagcgt ggtgatggag cactgcacgc ggttggccag
     9061 cgggtcatag tggaaacaca gcccctgcag cagcgcatca atcgcccggc ggcgcagcac
     9121 cggcggcata cgccggcgca ggccgcgcga acgggcatac gccacatgcg cggcaaaatc
     9181 aaaacgggag gtgaagccca cggccttttc catcagtttt tcgcagaact tcagcgttcc
     9241 ggcatcttca cggggtgtga acaccggatt cgggttcttt acctgacggt atgtgctttc
     9301 agtaaatgag gtctggttca gggccgccac aagctgtcgc ttatggcggg aacagattgc
     9361 cgtgcgttaa tgcactgcaa agggttgaat ctctgacacg gtttcatata tacttatccc
     9421 gtattctgtg aggcccccat tatctttctc tgattccgcc aagtttcgag gaaagatgat
     9481 gggggttttc ctttatggtc ttctgaaact tttttcagcc ttccggcccc ggcaaactgc
     9541 cgtcgtttct gccatacatc ctgaatgcct tatggcgcat tctgccgttt tctggctttc
     9601 cggaccgcga ggatcccaaa agccactgca taacgatccg caatataccc cttttccacc
     9661 gctttgaaaa gccgacacga tttctcagag ttacagttgc cctctttaac cccctaaaaa
     9721 gcgtcgtttt tttgaactct gtcaaaaaaa tgaacaattt cacaaacgat cttttatgct
     9781 gcttttttct tcttttccag tagaagagat cgccgaattt ttttaaacga tctaatatcc
     9841 cgcacacaac atcaagttta gaataatatt ctgtcttatc aaatataacg atctattcaa
     9901 ctgcttatct tcgctcttgc cacagataca tccttatgtg atatattttc cagccattaa
     9961 tcctgtttgc aggagttgtc ggttttcgcc cgcctcgaaa ccgtccacaa ggcttacaag
    10021 agtgcgtaaa tcatgtgcct gtcagaactg caggccaggc tgaacgtggg tcagccgctg
    10081 tttccgacac atgctttatg tattcgccgt tcacaacaac atcgaagccc ccctgcgggg
    10141 atcgattttc gcggagcgtt ccggattttg aatatcagtg aattacatca acgcaactgg
    10201 tatctggcac agtacatccc tgccggcaaa aaccgcgaac atctcttttc ctggctttcc
    10261 gaacaacacg tccttccctg gacacccctg atactgaaaa aggtccgccg cacggataaa
    10321 gtctgtggtt accgtcgaca tatacacgcc gtgtttcccg gttacttttt cctgaaagca
    10381 gaccccgaaa gccacagctt cactcacctt cgccgtcaca gtgcctttct ggacttcgtg
    10441 aaaatggccg gtgaaatcaa aaccgtcagg gaggatatcg ttcagtcttt gatgaaagtg
    10501 tacccggatc ctgcgctcaa tccggcagcc agggaggagc tggatgctgc ctccaccctc
    10561 tggctgacaa aagcgcgtta ccagtacctc ctccggcttg atgcacagcc tctgccggag
    10621 tcgaggatcg ccctgctcct gcacctggta tctgatgacg gagcgttgac atgaaaattg
    10681 ctgtcttaat ttgacgactt ttcccggcct ttatggccgg gatttttttc ccttctgttt
    10741 ccacaatgcc cggcaccggt gacacctgag ggtgtacgga gagcggctgt cgggcatttt
    10801 ttcaggacgc ctttatgacc attctatccc aggaaaaccc tgtcattctg ttactggaga
    10861 atatgctcga agcactctcc accgccccgg acaacataaa caacgaacgg cgtcgccggc
    10921 gttacctgct gaactggctt gacactgccc gtcagatgcg cgagttcaga ggaatggctg
    10981 aagagttcac taccctgcga aaactgctgg ccaccaattc agacataccg gtagcaaaca
    11041 ccctgaaatc actgctggaa agtggcaata acgcagcatt atacgacctg taccgcttcc
    11101 gctcggctct tgccacgtgc aggagtctgg gatggcagat tggcacgtgc gcatggaatg
    11161 accagttact gagtgagaca ctttcccgcg cacagaccgg gaaacgtcat atcctgcagc
    11221 tgaacagcac cggggacacc ttttccacca cgggcagaat gatcagaccg gtggcatttt
    11281 cattaatcca tccggccacc atcaacacgt ctgaggttga agagatattc cgggatgaag
    11341 gttttatgct cgcacacggg cgcgaatgca gtctgaacgg cagcggcaga aatatgctgt
    11401 cacgcattct tcatgtgggc cattcaggtt tagcggaaga ggaatggggc atggacaaca
    11461 gtctgctttt acactgaaat accgggacag ccacaacggc agccactctc cgctgacttt
    11521 ttttcgcaaa tcgcggtttg ctgatgtgga tgtcccattt ttgattatcc gtcctctccg
    11581 gttgtcataa agcgcattcc ggcgtgaggc ttctgttgtt atcatcccct cctgcagaca
    11641 tccccttgtc ttctgatgac tgacgatatg cttcttctga caattaaaga agcggaagct
    11701 tcacgccata atgttgtttg actgacctgc cacaggagga aaaatgacac tgacgttact
    11761 taccgcgtat aacgcaccct atctggcagc cctgactttt gttctgctta ctggtatcgc
    11821 tgaacttatc gccctgctct gcggacactc cctctcttca gctatggata cacctgattt
    11881 accggagggt ctgacaggag aggcgctgga ctggctgaac atcggacgga tccccctgct
    11941 gatcgtcctc tgtatgctgg caggtttttt tggtatatcc ggcattctcc tgcagggtct
    12001 tattattcat ctgctgcagg ctcctgctcc gaacattctc cttgcaccac tttgcctgct
    12061 gctgacatgt cctctcgtac accgcaccgg cagactgatt gccccctggt taccccgtga
    12121 tgaaacaacg gcgctcaccg aggaggcatt catcggctgt atggccacca tcactggtta
    12181 tcacgcagtg gcgggttccc cctgtgaagg tcgcttcaca gaccgcttcg ggcaggttca
    12241 ttacctgttg ctggagccgg aggccgggaa agaattcaga cgaggagaca aagtgattat
    12301 tgtctgccgt ctttccggtt cccgctggct gggcgaactg aatccctggc cggatgttct
    12361 ttaatcccga catatacaga ggttctcact atgtttaatc ttccgggatg gttgtttacc
    12421 gttatagcgc tggtcctcac cctgttcgtt atcgggctga tatttgcccg cctgtaccgc
    12481 cgggcatcgg cagagcaggc ctttgtccgc acaggtctgg gaggccaaaa agtggtaatg
    12541 agtggcggtg caattgttat gcccattttt cacgaaacaa ttcccgtcaa catgaacacc
    12601 ctgaagctgg aagtcagtcg tgcagcggct gaaagtctca taacccgcga ccgcatgcgt
    12661 gttgatgttg ccgttgcctt ttttcttcgg gtaaaaccat cagccgaagg aatatccact
    12721 gcagcccaga cgctgggaca acgcacgctc acacctgaag atctgcgtag tctggtcgag
    12781 gataaatttg ttgatgccct acgggccacg gcggccagaa tgagcatgca ggatttacag
    12841 gatgcccgcg agaactttgt tcagggtgtg cagaacaccg ttgcggaaga cttatccaaa
    12901 aacggtctgg agcttgaaag tgtgtcgctg acaagtttca accagactgc acgggtacat
    12961 tttaatcctg acaatgcctt tgatgctgaa ggcctgaccc ttctgaccca ggaaacagaa
    13021 cgtcgtcgtc gcgaacgcaa cgaagttgag caggatgttg aagtggccat cagggaaaaa
    13081 aaccgggatg cactctcccg acgtctggag attgaacagc aggaagcctt catgactctg
    13141 gagcaacaac aacgggtaaa aacccgtacg gcagagcaga gtgccagtat tgctgctatt
    13201 gaagctgaac gacgtcgtga agcagaatcc gcgcgaattc tggctgaacg taagatagag
    13261 gaagccgaaa tcgaacgaca gcagatagtg cgtacccgcc aggtcgaagc cgaacgggaa
    13321 gtggccatac gggaaattga gcaacagcag gcaaccgaaa tcgccagcca ggccagagcc
    13381 attgccgtgg cggcaaaatc ggaagagcag tcacaggcag aagcccgggc aagtaaagca
    13441 ctggctgagg ccgtacaggc ccagcaggat gtggaaacca cccgccagac tgcggaggcc
    13501 gaccgtacca aacaggttgc cctgattgct gctgctcagg aagctgaaac gaaagcggtt
    13561 gaactgacgg ttcgcgcaca ggctgaaaaa gaagcagccg aactccaggc agcagcaatt
    13621 attgaactgg cggaggccac ccgtcagaaa ggtctggcag aagcagaagc acagcgcgca
    13681 ctgaacgatg ccattaacgt gctttcggat gagcagacca gcctccgctt ccgtcttgcc
    13741 ctgctggatg cattacctgc cgtcatagag aagtccacag aaccaatgaa atccattgac
    13801 ggtattaaaa tcattcaggt ggatggtctg aatggcggtg ttacccgcgg taaaacagca
    13861 acaaccgaca taagtggcgg aaatctcgct gaacaggcag taaccgccgc tctggcttac
    13921 cgtacacagg ctccgctcat cgactccctg ctgaaagagg tgggtttatc cggtgaagga
    13981 cttacagccc tgacacgtcc actggtcatg cctgccccgg aaaaggttcc ggatgtcagc
    14041 gaaccggtaa ccgctccgga taactcagaa ccacaacagg tttaatcccc gtgccggaat
    14101 ttttccggca tctttttcac aaagaatgcc ctcacgggat attctggttt aaaagaggat
    14161 tgaaaatgtc cgcagaaaaa ttcagagttg tcatatacac caatacatgt gaaaagaaag
    14221 tcagttatat tcctgcaaca gaagatataa acatcgctat aatgccattt cagacggcag
    14281 acaatggctt aattattgtg tgtcagaact cccgtataat acgacgcaaa gagcgtattc
    14341 ccggaagcag ctgtgaatgg aaagatgtga cattagcacc aaaatccaga aaccgcgttc
    14401 agttatagcc tgacagcctg cttttcatcc cccataaaag ggggtaaaaa ttaaatattt
    14461 cacaaccgcg atatttttca ggaatgcaga ttattttctg ttatgaccaa ttgagaaaca
    14521 ggcatgagaa caatcacaac acgtgaacaa ttattaatta atgggagagt ccacgaaaga
    14581 atcgccacac atatagttac aggagctcat gggtatgaaa cgctttgcac aaatggctat
    14641 aacgtcagat acaacaaggc gcagcaactg gttgaaagct gcgaaaaaat agctgaaggt
    14701 aaactacctg ttacctgtcc tgtctgtttc accatatggc aggatgtaca cagattcacc
    14761 cacgttgact ttgacacaca atcggggaaa agtgatttta ttgatacctg ccattcggag
    14821 atcataaccg gacatgtttc aataacaggc aagaaataaa ataatgttcc agttctgaaa
    14881 aacaatatca cttgttttct ggaaactatc atttgcagca ggacgttttt ctttttatat
    14941 ttccggaacc ggaatgtttt agttttcata atcaccccga acatgaatgt tcatcatcaa
    15001 tatcctgatg aaattatatt tatctgaaga cctgttacca gccacctgtc ttataccgta
    15061 acccgataca taccggattt attctatgaa aaagaaatca cttacactcc ttctgccgtc
    15121 attactcagc ggctgtacgg catttaatgc cacgcctgtt cctgacacca cgcctccggc
    15181 tctggtgttc gtcgacggac aaattcgcga aagcgctgcc attatcgccc atacgcaaaa
    15241 acgtctgctt ccgccaccgg tgacccgccc tgtgacgccg ccccgcccga caacaacagc
    15301 gacgcccaca accgcagtca gcagccagag taccgcactg cctccccgtc cgctccttca
    15361 ggccacacag ctgcccgcac tggttatgac cggtaaaccc ggtcagtcat ccgtcgttta
    15421 tttacgtgag gcacatcagc gctccgtcag tgactggata cgccagttgc taccacccgg
    15481 ctggcagttc cggcaacaga acgctgccat accggcactg aacaaacgcc tccccgtctg
    15541 gtccgttaat gaccagtggc accgggcact gaatgcgctg ctggccggcc agcagcttta
    15601 cggacatatg gactggaaca ggaaaatact gactgtcacg acctctgcaa caccgccggt
    15661 cgacgtcacg gcaccgcagg gttcacagaa agctgctgat acaccgcgta atccgttcag
    15721 gggcagcacc gcaacgccgg caggcccgac accagtgaca cctgccgtca gcgtttcccg
    15781 ggctcagacc accaccggga catccggaac tccagagagc agaacaacca cgccagcccc
    15841 ggtggccacg aaaccggtag tgccaccaca gcagaaggtc ataccgccac ggcagtggaa
    15901 aatggaaaag gggaccaccc tgaaagacgg actggcccgc tggacatcat ccgaaaaatg
    15961 cccgcacggc acatggacac tgcgctggga aacacctgtc aactacagca tcgatgcccc
    16021 tctggttttc accggcagtt tccgccaggc gctggagcag ctctttgcac tctatacccc
    16081 ggctgaaaaa ccgctgtatg cccggaccag cactgcacaa tgcctgctcc gtgtgacaga
    16141 ccgcccgcag gaataaccta tgggatgggt tacgggtttc attatggtca ttatggccat
    16201 tggtaatttc tggttccaca gtcacctcag ccgtactgtg caccatcagc agaccgctga
    16261 aatcacacag caggctgcag actttattcg ttatatgaat gccatcaacg attacctgta
    16321 tcagcaccct gaacgacgtg ctgccggtgg tcagctcacg tcagcacagc tcggtctgcc
    16381 cgccacgaaa aacgtcagcc acctcatcag tcagcagcgc gtttttgtct gggcaaaaga
    16441 aaaacccggc cttatgggtg ccctcctgga acaaagtggt gattcggccc tgcttgccag
    16501 agtggaaaac ggtcgcctcc tggacacccg tgggcggcgt atttccatca cgctccctgc
    16561 tgtgatcccc gatcaggtca tcatctggat gaattaggcc ggactctgaa taactcacgg
    16621 atttatctat gaacaaaaaa cttcttgcta ttatgattgc agccactctg ctgcagggct
    16681 gtaccagttt ccgacaggca gagaacacca gaaaagacgc cgttgctgaa caacaacggg
    16741 cccgggaaat gatcgctgag ctgcgaaacc ggacaccggt tgtaaaggaa tccccacgtc
    16801 agtggatcaa tccccgtccg gtcacggata aaaaccccgg taatgcggtg cccggctgtc
    16861 cgattatcat taacacccgg cagagcatca cccttcagca ggtggcccag cggatctcgc
    16921 aaaactgtca tattcctgta aggatcaccc ctgatgtaca ggcgtatctg agcgtgacca
    16981 cctccggcag tacgcagcaa atccagggca cagtaccacc gccggatacc ggcggtatgg
    17041 ttccactggc cgccataggc agttctgaca cgacacttcc ccgcctgcag ggaaatacgg
    17101 cgtcacttcc tgaactgaag gcttcaggac tgacatcact gctgaatgcg gtcgcttccc
    17161 gtctcgggat ttcctggcgt tatgatggtt cacatatcac gttctattat cttgataccc
    17221 ggaccttccc ggtgacctat atggacagcc aggttgccta taatgccacc gtggtcagcg
    17281 gtaccatgtc atcaaatggc tcatcaggtg ggacagcatc ggatgccatt tccggtgatg
    17341 ccagtaatac ccagaccacc accgtggata tgaaatctgc gctgtatcag gatgtgaaaa
    17401 atgctgtctc atcaatgctg acgccgggta ttggtcgcat gttcatgtcg acgggcttta
    17461 tcaccgtcac ggacacccca caggtactgg aaacggtccg gacgttcatt gaaaaacgca
    17521 atgaagaaat gaagcgccag gtggtgctga atgtggaaat tctgagtatc cggaaaaccc
    17581 gtaatgagca ggcaggtatc gactggaacg cagtcttcag cgacagaacg ctgggactga
    17641 gcctcggcag cacattcacc agtgcagcca gcgataccgt cactggtggt gtttccattg
    17701 tgaacgggaa actcaccggt tcaaaagcct tcctgaaagc actctcttcc cagggagatg
    17761 tcagtgtggt aacccggaat tccgccgtga ccaaaaacct gaccccggta ccgatgcaga
    17821 ttgccaatca gcagagctac attgagagtg tgacgacgga taccaccgcc aatgtcggct
    17881 catccacctc cctgaatgcg gccaccatca ccaccggttt taatatgacc cttctgccgt
    17941 tcattctccc ggactcacag acactgcagc tgctctattc catgagtctc tccgacaaac
    18001 cggttattga gaattacgag agtggtggaa gcaaagccca gctgccgaat gtggatctga
    18061 aaaccatcaa ccagacagtg gacctgaaat ccgggcagac agtcattatc agtggcttcc
    18121 agcagtcagg gcgtcgcagt ggaaaacagg gtgtcggcac accgggattc ttcggtcttg
    18181 gcggtgggat caacagtgaa aacgacgaca ccattctcgt ggtgctgatc acgcccaata
    18241 tcatctgatt tgcagaagga ggactatcgt gacagatatt tcaccgacag agcgacaggt
    18301 gtttccgctg acggcaggac ggaatgtatt tcttgccgga atggactgga aaacactgcc
    18361 cgcaagccat aaaaaccccc ggacgtttgc ccgcagtctg ggggcagtac gctatatttc
    18421 ctgtgagtat ctcagtacgg aagataccga tcgtcacatc atggttgcag ccgtcagcca
    18481 gaatacaatc cccaaaggca gccgcaggta tttctctctc gccatgctta tcctgcctct
    18541 gctcgagcca ggaggatacg ctatcgttga actgtcacag gcaaacgata cagaacttta
    18601 tggttttgtt tctgctgttg acggcatcct ggtcagtgac ctggtgggaa cccgggagga
    18661 aatcagggag gcccgggaaa tattcctcac cattaacagc gcgcctgaac acggctggac
    18721 atgttatgaa ccgccctcgt ttaacgggcc tgacggcaga ggtcccctgc cactggaaac
    18781 cctgacaggt gcagggaaat atccggcaga agcacgcctt caccccgtat cccgtgggcc
    18841 acagcttatt ccggtattac tcatactcac ccttctgggt actgcatggt atggctggca
    18901 gtactatgaa cgcctgaagg cagaaaaagc agcacttgct gaagcggcac aaaaagccgc
    18961 agaggaacgg atcacaccac cgtggctgag tatgccggaa accggagatt ttatccgggg
    19021 atgcagtgac gtatggtcac gcctgccggt gtcggtcgcc ggctggcgat attcactcgc
    19081 cgagtgtgct gccaccggga acagcggcaa tctgcgtgca tcatatacaa acatcgccgg
    19141 gacgacagtg accgcattca gccagcgggt cagggaaatt ttcagcatcc ggccgtttat
    19201 ggttatgccg gacgggaata gcggtggttt tgccctgccg gtcaccttca gtatgcctga
    19261 aactcccgtt gccgttgagg cattaccgga aaacaccctc ctgcaggaac ggctgaccac
    19321 actcgcccgg tcgatgcagc tgaaaatgga ctggcaggaa gtcagcaaca gcttcaccga
    19381 tgaagacgga aataccatcc agcccccctg gaaggagtat gacctgcaga tactgacgac
    19441 acttccggcg caccaggtgg cagagcattt cagtgaaccc tccgtccgtt ttatctccgt
    19501 aacaaggcaa ctggaagagg gtcgtttccg ttatcaattc acaggtaaat attatgtcca
    19561 gtaaaacaaa agctcttctg ctttccgtcc tgatcctgcc gacaacggtc gcggccagca
    19621 ccatcagcaa catgcaacag aataaccggc aactccaggt ggaacagatt caggaagaaa
    19681 ctgcactgta tgaagccatg ctggcaagag aaaaggtgcg acatgaactc agaaccctgg
    19741 agcagggaga accagctgag cctgtgcatt cacaggaaag cagttcagcc gtcacgacag
    19801 atacccctgc ccccattacc ggtattcccc ggctgacaga gatatccgga gttcaccccc
    19861 agataaccgc caggatacaa ctgccgggcg gacaaactgc tgatgtaaaa gaagggcaac
    19921 acattcccgg caccacattc cgcgtggaaa agatcaccac tgacagtgtt gtccttgaac
    19981 aaaatggtat cagaacaaca ctacaaccct gactcagtgc cgccatttac agccggctgt
    20041 cggaacagat tattcccccg gcccttaatc acaaaaaaaa tcgtttccgg gtaccgctgc
    20101 ccgtaaggag attttatgct ggcacctgca gaccagccac ctgatgatat tctggattac
    20161 gtggttgttg atacagataa aacaggacaa attcatgtca gagtggtgga aggaaaaaaa
    20221 caccttcgtg ccgtccagga ttatctgacc cgcctgtgct cccgctatcc gggtaaagta
    20281 tccccgtttg agttcaccac acttgatgtg atcgcccgac tcaggcagga gacggtcatt
    20341 tccggggtgg aaaacggaat taaccccgta cagcaaaaaa tgctcgggta tatcagccat
    20401 gcggccaccc tcagcgcctc agatcttcac atcacgccgg ggcgtgacag tacggatttc
    20461 acgtatctgg aggcccgtgt ccacggagag ctggagctgc tcgatatcgt cagaaaagat
    20521 gaggggctgg aattactggg ggccacctat tccggaatga ctgacgtcat aaagggaaca
    20581 cagtttgatc ccggtgtccc ccaggatgcc cgtctggctg aaagattcct gaaactggcc
    20641 ggactgtttg gtgcgcgcta ctcccactat ccgtgtgtgg gcggactcta tgccgtactg
    20701 cgtctgatca aggatgacag tcagcatata ccgacattca gtatgctggg gtatcaccct
    20761 gagcaggagc gcgctgtccg aaggatgctg cagcgaccgg agggcatcgt gatcctctct
    20821 ggccccaccg gttccggaaa atccaccacg ctgaggacgg cttctgccgc ctatctggaa
    20881 cagtatggct tcaacgatac cggagggatc ctgctccccc ggcgacgtct gttcacaata
    20941 gaatcaccac cagaggggcg tatccccggt gcaattcaga cggccgttat ggataccgct
    21001 cagggctggg tcgattctgt taagtccgcc ctgcgccttg atcctgacgc tatcctgaac
    21061 ggggagatac gtgaccatgc ctccgccatc acggcgataa aagccgccat gaccggtcac
    21121 ctgatgctga ccacccttca tgctaacgac ccgataaata ttcttgagcg tctggaaatg
    21181 gagggcgttc aggcaaggat gattgcagat ccgcagttat ttatcggcct gctcagccag
    21241 cgtctggttc aggtgatatg tccccactgc cgcctcccct ggcatgaggt ggagagctcc
    21301 cggacggatg aagaacgacg cctggtggaa aacttctgcc agcccgatgc cgtgtatctt
    21361 cgtaaccata acggctgccc acactgctgg cgtggagtga acggccggac cgtcattgca
    21421 gaagtgattt ccccggatgc gaagtttttc cagatttacc gggaaaaagg ccggattgag
    21481 gcaaaaacct actggcaccg ggagctcggc ggtatgacac gaaatcagca cctgctgggc
    21541 aaaatcaaca gtggtcaggt cgatccgctg gctgcgcatt acatatcccc tgtagacgag
    21601 gacagctata cgctgctgca ctgatatgaa aattctggaa agactcaaca atacggaact
    21661 ggaactgtcc ggccttaacc gctggctggg caaaaagacg ttcaggcgga ctacgtttta
    21721 cgagtccctt gcaggcatga tccgggacgg cacaccggtt atgcgggcac tggagttcat
    21781 ctgcgatgtg gaaacggatt ttggtaaaaa gaaagggcag tccggcctgt attttctggc
    21841 gacagactgt atcgccagca tacgcagcag tggtcagctc tctccggccc tgaaggactg
    21901 ggtacccaaa gatgaaattg ccctgatccg taacggtgaa gaacgaggtg atatcgctga
    21961 agccatgttt caggtcgtca aaacggcaaa aggacgacag gagatgatta gcagtctggt
    22021 gagtgtctgc ctctatccgc tcatcctgct gacactctgt gtggtgaata tgtataacgt
    22081 tcatacccgg gttattccga tgatcagcgc ctttgcaccg gaagaacgct ggtcagtaca
    22141 gatggtcatt ctggccaaat cctccggact ggttatcggt tatggtttgt ggctgctggc
    22201 aggactgctg ctgctggcaa ttctcatcag gatgtcattc agtctgtata ccggtccccg
    22261 acgacggtat cttgatcgca tcccgccatg gtccctgtat cagactttcc atggcgtgaa
    22321 ttaccttttt aatatttcat ccatgctgca gataaatatt cctttatctc atgcccttac
    22381 cagaatagaa aaacatgcag aacataaccg atggttaaaa tcacgaataa gtgctatcag
    22441 aaaaaatgtc ttatccggtc agtcactggc actggccatg aaaaactgtg gttatgactt
    22501 tccgtcaaaa tcctgtgtga ataatcttct tttaaattca gaaagggagg acagtgttgc
    22561 cagtatggcg ctttatgcag accgctggct ggaagaagca aaaaagaatg tcagaaaaag
    22621 cggcattatt attactgcta tatcaggtct gctggtattt ctcttcatca tcaatatggt
    22681 cagtgccatt tacggcatca gtgacatgct gaagcagtaa acacagcatc ctttatattc
    22741 ccctcgatcc aaattctgtt ttaccaatct caggagctaa tatgagcgtc ataacctctt
    22801 cgtattcaga acataatatt cgtcaccttc agaacgatga taaaggcatg accctgctgg
    22861 aagttctcgg tgttctggtc gtcgctgcca ttgttattgg tgccgtgatg ggactgatgt
    22921 ccgataccct gtcctcttca gacaaccaga aggaactcaa aaacctgcag acaatcgcca
    22981 ccaaaatgaa agcccaaaaa ttccaggggc agtatacagg tactgactac gtcaaaatcc
    23041 tgaccgaaag tggtggcctg cctgccgata tgatcgccgg tggtaataag gcgaaaaatg
    23101 cctggggtgg tgcggtaacc atcaaagtca gttcagataa atactcctac gttattgaat
    23161 ccagcaatgt gccgaagaaa aactgtattg atctggtcac cagcctgcgc agttcatcca
    23221 tgtttacaaa aatcaacggc aatgtgacga ataaagttga cccgtctaca gtatgtaacg
    23281 cagataaaac tactatcaaa ctggaaacca acagttaatc attcatcagc gcccgcccgg
    23341 gcgctttatt taaggtcact gtatgaacag gttatttatc ctgacagtga ttttttcact
    23401 gttatgctca cttccttccg cacaggcatc cccacaggac aaacaatatg atcgttgttt
    23461 tattaccgcc ggaaaacgtt attccattga tccaggatta ctcaaaatta ttgcccgcag
    23521 ggaaagcagt ctgaatcccc gggccattaa tcataacagg aatgacagag gaaaaattat
    23581 cagtaccgat tacgggataa tgcagattaa ctccacacat attcccgggc tgaaaaaaat
    23641 gggcattatc cggagtgaga aagatctgtt gaataatgtc tgcctgaata ttcagaccgg
    23701 agcatggatc ctcgcccggc attttcagcg atgcggcgta aactgggaat gcctcggcag
    23761 ttataacgcc ggcttcagta aaagtaacac tcaccgccgg atgaaatacg cccggcagat
    23821 ttacagcgct tacatgcagg gacgttagtc cgggagaact gccatgctcc agataatccc
    23881 ggcactgctg gtcgccataa ccgtggtaat acagtgtctg gccgttcaga tgagtcgcta
    23941 tcgactctcc cggatgaatg cccgcccccc tcaccatggg ctggcgctga tgagcgggat
    24001 gcttgcagct gccgccatta tcctgcgtct ttatcagaat gacagcgctg tcggggtgct
    24061 gtttatgacg ataatgaccg gatgtatgac actgatgacc ctgacggatg ctgcatcctg
    24121 ccgtcttccc cggcccttca ccatcatgta tctgataagc ggcagcgcat ttcgtctctg
    24181 gcagggagac tggctgacag gcctgtgcag tgcagggttc tggttcctgc tgttgcttct
    24241 gttcaggcaa tacacctctt accgcaggaa aacagaaacc ataggcctgg gcgatgtttt
    24301 tctgattgcc ggtattgcca tctggtcccc gccgggagac atcccctgga ttgtcgcaac
    24361 ggcagcaggc ggagccttac tttatctgtg gtgtaacaga caacgacgca ttaccagaga
    24421 acttcccttt gggccgtttc tctgcgcgtc acagtacgca tttacttttt atcccggtgg
    24481 cctttggtaa taccggaggg aacgactatg caaaaagaca acgataaagg ttttgcactt
    24541 cttgaaattc tgggaggact ggttgtcatc agcctgctca tgccgctgtt ctggtcttac
    24601 attgaagact acctgaatga aatgcgcaac cagagcgccg catttcatgc agatgcctac
    24661 aatactgcag ccaggaccta catcgccgat aacaatgccc gcctgcatag cggaacgctt
    24721 cctgccacct tcacggcgga tgagttaatc cggaaaggat atctgaaggg tcttaaccgc
    24781 tcgccctttg ggcagagtta taccaccggc atccgtcgta acacgagtac cggtcgtctg
    24841 gaggccctga cctgctccac cggaggggag aatatcaaag acgatgccct gcgcagtatc
    24901 gccagcctgc tccccggtct gggcggattt ataggaaaaa atggcacagc aaccggtgtt
    24961 tttggtggat ggacagataa acccggcgac tatggactca gctgcaatgg cgggcacatc
    25021 gccatcgtaa tgatgggtga tgacctgcag gaaagcgatc gcctgtatcg ctttcaggta
    25081 ccgggacgac cggagctgaa ccagatgaac acggccatca atatgggagg gaacaacctg
    25141 aacaatgcag gaaacgtcaa tggacaaagt gcaacactga aaggtgatgt tacaagcgaa
    25201 aacggctggt taatcaccaa aaatgataaa ggctggaaga acatcaccta tggtggcggt
    25261 tttacgatga cagacagcca gtggatacgc gcagtcggtg gtaaaggaat tataaccaca
    25321 ggggaaatca aaggaggaaa agtcagtggg gggacagtcc ggtctgacgg tcgcctttcc
    25381 acaggtgagt atttacagct ggataaaaca gccgtagcaa atacaaagtg ttcaccagat
    25441 ggacttgtag gccgcgacag caaaggggca atactttcgt gccaatccgg tgtgtgggtt
    25501 ggctttgaat cctaccctgt gggaagccct atcccgtggc catctgccac tcctccacaa
    25561 ggctatctgt tgatgaacgg ccagtcgttt tcctgttccc ggtatcctca gcttgcccgt
    25621 gcttatccta gctgtaagtt acccgatctg cgcggtgttt ttatccgtgg ctgggataac
    25681 gggaagggac ttgatggtga tcggaacaga cagctcctga gttatcaggc cgatcaatca
    25741 ggcgtctatc acgaacgtgg cggatggctg aaagggcatc atagtggtat gccatactgg
    25801 gcacagggga gtaccacaga aatgcgtccc aaaaacattg cattcaacta cattgttaaa
    25861 gccagctagg gactcacatc tgataggtat acacagagaa aacaccgtta ccacaggcgt
    25921 aattttgtgt tggtctggag atgatttgat atgacgctcc cactggcacg gcaaatgaaa
    25981 taaatctccg ggcatatcag ctggtcagca aactcgatgc caggcgtaaa cagaccagca
    26041 aaatttcccc ttattttgtt ccctaccctg caactgtcgg gtgcagaaac gggctgtttg
    26101 tcgtgacact gcacgatttc gaccttgaaa cccgtgcaga aacccgggga ctggcaatat
    26161 ctcatgccag catgctcagt cacctcctgt ccgaatcctt ttcattcgcc cttaaaacag
    26221 agttgcaaac actcttgaaa cacactcgat cataacgatc gaaataactc gcttccgttt
    26281 aaaaaacact ctctttagac tcaatttatg aacaaaacac gctgacccgc atggaggtga
    26341 aatgaaaact gacagcacag gtattgccgc ccgtatgatg ctctccctgg acagggagcg
    26401 aatttgcgaa tgcctgctca gtcatcgcca gctgcagtcc acacccctgc aggttcgtta
    26461 tccgcaggga gtcagggatg cccttggtat catgagcgaa cagctttctc tttccgtctc
    26521 tgacctgacg cgtatcctgg tcgaagacgc gttaagtgaa atgtttcttc ctgcagataa
    26581 catcgtccgc cgtcttctca gcaggatgga gcacatcatg caggcacatg atatcagcgc
    26641 aacaacgatg gctgcattac tggccccctg gaatatccgc cctgccgtct tccgggagcc
    26701 ggacagactc actgactatc tgaccgggga gattcttgca gcactggctg actggtttta
    26761 tctcagtccg gaatggctga acggcagggt gcattatcca ctgtatcgtc cgggagactg
    26821 gccagccaca caggagatat tctgcaggat tatttcagcc cgcgagaata tggacattat
    26881 tctctggcat ggttttccgt ttgccggaac gcattccgga gaatattgcg gcgttctgct
    26941 caggcagaaa aaagaaatta ataacaccat tatttatccg gtactttcgt tatatccggc
    27001 cagaatggat atagaaaaag aaggctggtt tcagatggcc cgaaaaatat ctccggatat
    27061 ccctgttcgc gccgtaacac tcactccggc acaggcagaa tatctgataa caggaaaaat
    27121 attacccact gcgttattca gggttccgtt atccccctgg taataatact gagcaataaa
    27181 ccggaataat acaccaactg tttttaatgg cacagccgtg ccctctgttg ttatacccaa
    27241 aatacctcaa tacaggaaac tgaaatgatg atgaaaaagc atattacccg tactctcatc
    27301 gcgtctgccg ttttattttc ctttaattcg actgcagcaa cgtcatattt tgaagcccgc
    27361 aatgatgcga tgggaggaac gggggttgcc tcctctcact atggtgtggc cccactggca
    27421 aacccggcac tgctgacaaa atccggtgca aaagatgact tcagtcttct gcttccgtcc
    27481 gtgggtgcgc agctgtccga tccgggtaat atcgccgaca acgccgacaa aatcagcgat
    27541 gactggaaag ccttcgaccg tgccgtcgac agccactccg gggtaccgca gacagcggcc
    27601 cgcctgaaag agagactgca ggacttccgg aacacccatg ccagcgcaca ggccggggta
    27661 tctgctgtgg cggcccttcc cggtgatacc ctcgctgctg cactgatgct gaaaacgctc
    27721 gggactgtca gcgtcgacgg taaagtcagc gatgccgacc agaactatct ggaaagcatt
    27781 gcagacagtg gcagccagga tgtggataaa aacagactga cctcacaggc ttttgcccgt
    27841 gccgcactga tcaccgatgt gggcgttgcg ctggccactg aactggaaac ggccggacaa
    27901 aaatggtcac tgggcttcac accaaaattc cagcgtgttg acctgtttaa ctacaatacg
    27961 ctgatcaaaa actatgacag cagcgcattc aaaggtgacc gttaccacaa cacccaaaac
    28021 ggtattaacg ctgatatcgg tgccagcatg gacctggatg acaactggac gctgggtctg
    28081 gtcgcacaga acctgatccc acgcagcatt gagaccaaag aggtacatgg tgtcactgaa
    28141 accttcagga tccgtccgca agtaacaacc ggcgtatcat ggcacaacga catgttcacc
    28201 acggcactgg atgtggatct gaccccggcc agcggcttca catcagacag caaacgtcag
    28261 tttgccgccg taggtgctga gtttaatgcc tggaaatggg cacagttacg taccggttac
    28321 cgccagaatc tggccagcaa caacggcagt gcattcactg ccgggattgg gctctcaccg
    28381 tttgatgtga ttcacattga tgtcgccggc ctcgtgggca ccgacaacaa ctacggtgcc
    28441 attgctcagt tacagttcac cttctgatta tcacgccccg ggacgcccgg ggcttctgac
    28501 ctgaatctgc tcgtttccgc tcttctggcc actcaccgcc agtcctgttc attattttcc
    28561 ggataaaacc atgcaaaaaa aacgtatcac ccttgcaggg ggagttgcca tgctctgtct
    28621 gagcggctgt catcttcccc cggataatac cgcaccggcc aggaagtacg gtgcagcttc
    28681 tgaagtcgct gtcacacgac atgcacagta tcagttgtgg gagcagggca cgtattctgc
    28741 agccctgcct tcccgccagc ccggaggcag cctgcaggct cacagcgggc gggtttcctt
    28801 tgactgggaa ggcgatgctc tggagctcct gagcgagctg gcccgtctgc gcggacaaca
    28861 gttcagttac agcggcgtgc gtcttcccct gcccgtctca ctccatgtac gcgatatgac
    28921 cttcgctgat gcccttcgtc tcacagaggc ccagactgcc tggcgggcag tcctctcaca
    28981 gtcagccggg cgtttacatc tttcctatat gccaacggag aaaaaatgaa aaaacaggtc
    29041 tgtctcctgc tgtccgggct cctgctggca ggctgtgccg gacagcacca tcgcccccct
    29101 gccggtatgg caggcaattt accaccgccg gatctccggg catggctgac gcccactgac
    29161 aaccaggatg acggcatttc agacacccgt cgccagatgc tcacggacgc cggacgaacg
    29221 ctgggattcc gtggcggaaa agcacagcgc tcatgggaac tgattcaggc actgaatgcc
    29281 cgggaaagta cactgaatgc cctgtatgac ttccgtcccc ttatcagcag ggaaggctgg
    29341 ctaccccctg ttattgatga agcgcaggat gttgctcaca ttcagcctga ccagataaga
    29401 accgcgtccc gtgtctggac catcctgcgc cccgaacgtt ttgtcagtaa cccgccgggc
    29461 tggcgtgact ggcttctgcg ggggctttcc actaccgcca ccccgggaac tgagggccgt
    29521 gttgtaccgg aagaccgcgc acagcgcaga ctctgggaaa acgcactccg ccagggctgg
    29581 caggaaggcc gtgacaacgc tgacctcacc ctggaagcca accagaaacg cctgacccgg
    29641 gattaccggg gaatgatgct ttatgccctg ctctggcgtc agggcatgat cacacgcccg
    29701 gatgtgactg aacagcggca gaccgtcacc ggtaacggca ggaaactcat caccggagac
    29761 cacgtccgcc gtctgaagac acacgcggaa ttcaccctgc agaaatcacg ctggcgtccg
    29821 gtcgtcagca cggaaggagc ccccgatgaa attacccgat gagttcggca cattcccttt
    29881 cgcccgtttc acggccgaag agtttcgcta tttttttgcc tggtgtgccc gacaccgcgt
    29941 cagtgatatc gatctgacag gcggctcacc ggtatctgtc agtcgctttg gacggcgggt
    30001 acgttcgtcc gcacatcctc tgccgtcctc catcatgatg gcgctgagtg atgaattgtt
    30061 tggacgggaa gtccttcccc gggtgctgtc cggtcatccc gtggaccgta ccattcaggt
    30121 cagcggcgat gcctccggtc gctacggtct gcaacggggg cagcgtgttc gcctgcgctg
    30181 tcatctgatt cagggaacat cagcaacaga agaaaaagta atctccgtca ccatgcgggt
    30241 gatcccgtcc gatattccgg acatcctcac catgaatatt gaaccggacc tgctggatgc
    30301 catgctccgg aaaaccggtc tgggtttcat ctgcggagag accggctccg gtaaatccac
    30361 actggcagcg gcactgtacc gctacattca gacgcatttc cccgaccgca aaacggtgac
    30421 ctatgaagat ccggtggaat acattctggg gggagagaat gaccttctgc caccccatca
    30481 ggccgaaatc gggcgtgatg tggaaagttt tgcggcagga cttcgctctg ccgttcgccg
    30541 taacccggac atcatcggta tcggtgaaat cagggattat gaaacggcag atgcagccgt
    30601 ccaggcaggt aacaccggtc acttctgcat cggcaccatg cacaccaaat cacccggtga
    30661 aacctttgcc cggctgctgg ggttatttcc accggaaatc agggattcaa tggcagccgc
    30721 caccctttcc ctggtgcagt tcattctggt gcaggtcctg gtcagaacaa atgacggtgg
    30781 cagacaggcc gtccgggaat acatcgtcat aactgacgaa ctgcgtgata cgctctcccg
    30841 gcagtcccat gcgacatggg ggcattacat tgatgaaatc attcgcaagg aaaaacggcg
    30901 gatacgggat caggttctga ccatgtatca gacgggcagc attgaggcat cagaagctgc
    30961 gctcttcatc ccggccggag aattccccaa atgaaacgcg aaggcttctg gcgggatgtc
    31021 actccgccac tgaatattta tggtgtcccg ttgccgttta ccatgattta cctgctgctg
    31081 tttccgtatc cgtcaaaaac aacattctgg gtgtgcagcg gcattttgct gttttttgtg
    31141 ctcctggggt actcaggatg gactgtccgg acgctctgcc ggcgtctgta cagcctggtg
    31201 cagggtaaac aggcgtcagg ccgtccctgg tggtaccggc atcgcacaga attaccccgt
    31261 gaatggcgcg gaacggaata accctctttt gttcatcatc ccggcgtatc acaacgccct
    31321 catctttaat ccccggagaa cgacatgaag tccagatatc gaacctggct tgctgtcccg
    31381 cctgaggaga cagaagccgt aaaaaatgca gttcccccgt taaatggccg caaagctgtc
    31441 gcatgggatc cagaaaaaaa gctctggtat gcccgggcgg gtaccgaact ttcactgctg
    31501 gagcgctggt taccccggcc acaggaactg tcgatggatg ccggcgatcc ggtcactgaa
    31561 tttgctcagg tcctggaaaa cgccgggctg gttattcagg gattgccaca gatggacgga
    31621 gccatacaca gagtggccac ccgggatgac aaaaaagggg ccaaaagtgg cgcatacagg
    31681 gcctatcttg atggtcgtcc cgccggctgg taccgcgact accggagcgc cgatgacagt
    31741 cccacaaact gggtattttc cggcggagaa caacacgatc ctctggccag actgcatctg
    31801 cgggcattcg ctcagcaaca gcgcgatgat aacgctcgta aactgcagca acagtataac
    31861 aaacaggccg gctatgcccg ctcctatatt aacaggttgc cacaggcaac agcacatgaa
    31921 tacctgacgc gcaagggcat ccgggccgcg cctggcgtgc ggctgaacaa taaaaatgag
    31981 ctggttattc ccttcagcaa cggccgggga gaaatacgtt cgtaccagcg tattccggtc
    32041 accggcggga aggatgcccg cattctgaaa gactccgaga aaaccggcaa ctggttcacc
    32101 ttcggaaccc cggaaaatgg ccgccccctg ttatttgcgg aaggttacgc tactgccgcc
    32161 tccctgcacg aagcaaccgg ccttccggtt ctgatgacag ttgatgccag caatatgatt
    32221 gccgtggcag aaaatgcccg ccaaatctgg actgacagcc cctttgtctt ctgtgccgac
    32281 aacgatcatc aacgggaaat taacaaaggg gtttttagtg caacaaaggc cgcagaagtc
    32341 acgaatggcg aagtcattat tccggccttt acggaggcgg aaaaagcaca gggactgacc
    32401 gatttcaatg acctggacgc cagccgggga cgggacaact ttcagaatgc catgaatgca
    32461 cagttaaaac atatcggtat ccccacaccc aacagtgaca ctgccgacca tcgtgaggct
    32521 gtcgtgatcg acaacctgat attcacaccg gtaaaagatg agaagccaca aatgtcccct
    32581 gaaaacagac agagcacagc cccggaaacg gagctggcga cacaggatac gccttatgac
    32641 acccactccg acgatacaga aaaccaggca tatgaagatg actatgccgc ctatgcatcc
    32701 ctgcaatcag taactgcgga gacgttatcc ccggataaac cactggagaa tattgcgtct
    32761 gccgctgata ccggcacaga gaatacctct gtgcctaccg ctggcgacca ggaagacctc
    32821 aatacagacg ccacaggtac agacaccgct cacaacagcg acacagcaga aacaggaacg
    32881 cttcctgaac ctgccgggat gcagtccgtg cagcaggcaa ccactgaacc gaccgcagaa
    32941 gataaaccac acgagaacgt tgtttccgct gccgatacca gagaagaaaa tgcctctgtg
    33001 gctaccgctg ataccggtga agaaaatgcc tctgccacca ccgctgacaa ccagagcacg
    33061 caggcagaag aacatccccg gagtaaagac acaccggaaa cctccctctc cgacgcggcc
    33121 gggacccagc acaccaccac aacgcagtct gaagaacaag tgcaggtttc catggccagc
    33181 gctgacgact ggagcgcatt tgcggaagat ctcaacgctg acactccggg ggcccccact
    33241 gttcacaaca ccgacaacgc ggagacaaac acagtttctg aaccggccgg gatgcggcca
    33301 gttcagcagg tgaccactga actgacggca gaagataaag cacaggaaaa gagcgttgcg
    33361 tctgccgctg agaccggcgc agagcactcc tctgacatca ccgatgacaa caggaacgca
    33421 cccgctgagg agctcaatgc cgatgccacg gttgctgttc acaacaccgg cacaacagaa
    33481 acagcctcac cttctgaacc tgcagaaact cagccagtcc ggcaggccac cgctgaaccg
    33541 acggctgaag ataaactgca ggaagagcgt cttgtttccg ccactgagac cagcacagcg
    33601 aatgaagcag cccgccctga aacgccatcg cttttcagcc gtctgcgccg ttttctgacc
    33661 ggtgtgaaat cacgcacctc agaaccttcc gttattcagg agaaaaagcc cacggaggct
    33721 gaaacagcaa cagaggaggt cgcaccacca tcaccaccgg aaaccagccc ggaagacaca
    33781 tcgtcagcgg aaccggacac gattatcttt gcaccccgcc gtcccgacag tccgcagcgg
    33841 cacaacctgg acgagatcct taaaaatctt acacacaaag agtttcccga caggactgcg
    33901 ctctacagcg ttgacggtga acccgccttc ctggacagac tctactgtct cgaaatggtg
    33961 gatggcgcca gcgcggatga caaaaaggtc ctggctgccc ttgctgtggc caccaacttc
    34021 tatggcggcg ttatcgagct gaccggcagt gatgccttta agcaaaaagc catgcagctg
    34081 attgtcgaat acgacatcaa ggttcgcatg aaactcccgg cccagcgggc agcactggaa
    34141 aaaatccgca aggaaaaaga gatggcgcat gatgccattg tcacccacag acccacgccg
    34201 gaactcaacc gccagtcacc ggaagagaca acaatggccc aaccggctcc ccctgtgtca
    34261 ccgaaggcag aggctgaaca aacagcaggc agtacggctg aaacaccgca tccggaagcg
    34321 ccggtcacgg caaaagctga agccccccgg gccgacagac cggaacaacc cgccgccccg
    34381 tcgtctgcgc cagaaaaagc ccctgtcagt gcacagccgc agcaacccga cacaccggag
    34441 gaagagccgc ccggcaagct cagccccggt gaaagtgtga ctgccgtcct gacaaactac
    34501 ggagaaaaag agtatgcgcc cggaaaaggg cgctgctttt ttgtggaact caccaaccgc
    34561 agtggcaaac gggaatactg gggaaacggc ctggaagaac tggtcaaaaa ccatcagaag
    34621 ggtgaccccg tcacactgac actgcaggcg agagaaacat tccggctgtc cggtgaaacg
    34681 cgggatcgta tccgtaatat ctgggccata accccggtca gtaacggcat taccgttaat
    34741 aatgaccaac ctcatgcggg tcagcatacc ctggagtttc cgatggcaac attcagccag
    34801 gtgatgttac agatgcaaca acactggcca cagataatgc aggaagttcg ctcccccaaa
    34861 aatcctccca ataatctgta cattggtgaa gacaggcaac ctgcatcagc acctcaggat
    34921 caatcaatgc gactccccct cgctggtaat gcccccaatg aactgatacc agccatcgcc
    34981 tctgccgata aggacaatcg tcagttgaat cttctgctgg tccacagcgc agatgatcat
    35041 ctgcagggcg tggtccgcct gaacggcacc ctttacccgg cactggccac cccgtcagct
    35101 gacaaccgtc agctggttat caatgccctg acggataacg gtctgcagtt tgccggttac
    35161 ggtgaagctg ttaaccatga tgagaataat catcagcgtc cgtccccaca aataatgcag
    35221 tttcacctga agcaacagga ctccccgttg tttgccgcca tccacaaacc ggaagagcaa
    35281 ccggacaagc ttttccgctc tctgggtttt gagcagacct ggaaagagtg gtctgacagc
    35341 caaaaggcgg aggacaggca ggaaaaaact ctgcaacagg cacaatccca cagccccggc
    35401 cgatgagccg gtaaggacga ccatgaacat gaaaaaaacg ctgctggtca tactgaccgg
    35461 catcaccctg gcatcaccgg tggcaggtgc cgccacctgc cgggcggggg ccgctgccga
    35521 agccggcagc agagccgcct gggagcgggc gcgcacagcc agccaggcat ggtcacagcg
    35581 ggagaatgac gtcgcctcct ccctgcagtc ctgcctgtcc cgcatcaagg acatcagtat
    35641 cactcttccg cagttcccca gcctggacaa gctcctccgc gacctggaaa accaggtctg
    35701 tgatgccgtc gtggatcggg tgaatgacca tcttccgggg aatatcgacc cctggaaaga
    35761 ctacaacctc tgaacaggag caccatgagc gaagcacaac ccacctcccc tgtctcaccg
    35821 gaagcggata acagcggaga tatttataac cccgtgctca gaaccatcgc acagtcagaa
    35881 caacgggccg cgctggtacc cggtctggtc agggcgttgc tgtacagcgg cgcagcactc
    35941 tgcatcagcc tggtggctaa tggcatacag tactggcatg ccaccggcgt gacgcgcgaa
    36001 tactttgcca ctgacaatgg ccgactggtc cgtctggcac caaccagcca gccggcctgg
    36061 agtcagagcg acgtcatgaa ctttggcagc atgacgctga gcgaagcctt caatctcgat
    36121 tttgtccact accgaaaaca aatcacgaca caggcccccc gcttttccga agccggcttt
    36181 gccggctatg tgaatgccct tcaggcctcg aacattctcg acactattaa aaaggagaaa
    36241 tttaacctca caaccactat cggggcaggt accctggtca accagggaca gatggagaac
    36301 ggcgtctggt tctggaaatt ccagtatccg gtcaggatgc gcctggtcgg tcagaccagc
    36361 acccgccccg aacaggcctt cacctttgaa atcactgtcc ggcgcgttga cccccgcatc
    36421 aaacccggcg gcatggaaat cagccagatg gtttcccgta acgccgcagc aggaatctga
    36481 gactcatatc atgcacatac atcatcttat tggcgcactg cttattgtgt gcggcactgt
    36541 cacagctgca gataacccgg gctggaaaaa tgcacgaagc ccgcagaccg gcggaaagac
    36601 cgcacaggca accgggaaca ccccggcagg cggtcccccg ccagccgcac aactcccggg
    36661 aaacctgccg cctcccggcg ctgctcttgt acaggaaggc gcatcgctgg attctcccct
    36721 gaatgccagt gaaattcgtg aactccgtgg gataatggcc gataacgaac gggccatcag
    36781 tgccccgctc accagtgtgg tgccccgtat cagttccctc acggtgaatc tgtctccggg
    36841 ggccagcctg ccccttgtcc gtaccgctct gcataacgtc agcgtggtga ccttcacgga
    36901 cgtcaacggc agtccgtggc cacagtcgga tccgccctat aacgctgcgc ccggtcactt
    36961 tgacgtgcag ttcaacggca atatggtgtc cgtcaccccc ctgcgcgcct gggctgccgg
    37021 gaatgtgacg gtctatctca aaggattgtc tgtaccggtc atcctgaata tcaccagtgg
    37081 tgaaacggac accactaccc tgagtcagga aatcgacagt cgtctggatc tgcgcattcc
    37141 ccggcgggga cctgccagcc cggtggtgtc cataccgtca gaaaaaattg ccctgcatga
    37201 cgccactctg caggcctttc tggacggtgt gccaccggat gatcccgcca tccgccgcct
    37261 gaaattctcc ggtaacgtcc cggacaccac catctggcaa cacggcgatg atcttctcgt
    37321 gcgctcacgg gccatgctgc gggatgaatt tgaacagacg ctggcatcgg ctgatggtac
    37381 gcggctgtgg aaactgccgg tgaccccctt actcaccttc tccgtgaatg gtcagtccgt
    37441 tcacgtcacc ccggaactgg agtaaatcat gagcgcagaa caagaacagg acacagccgc
    37501 aaaatccggc aaaaagctgt cagccctgct ggttatgggg ggcgtgatta tcatcggggg
    37561 cggatacctg ctgctcacct ggctgtcccc cgaaaacgct gcatcgctct cgtcagtcaa
    37621 tatcaacagc acaacgtccg gcggcggacg gaacgtgatg gagacacagc actaccggga
    37681 tttactgcgg gctgacaatg aacgtggtgc tgcagaagcg ttacgtaata acgagacgtt
    37741 tatcgccagt ctgccccggg gactggatac cgataccgga aacgatcaga aaagggataa
    37801 tccccccgat gatcagtcag gcacttccgg gcaaaacgca tccccccctc aggaagatca
    37861 gactgcccgg gaaaaacggc gtgaggcatt acagaaactg cttgcccgta tcaatacaca
    37921 gcatcaggat gcccggccac cggtagtggc agcagccatg tggagcacca ccggcactcc
    37981 cgggacagcc ccacatgcgt caccgtccgg taccgttaca tcacagcatc cccgtctgca
    38041 ggagacgagt ctggcaacac cggctacacg tcaggggatc cagctgatac cggcactgac
    38101 ccgcgtaccg gcttacatcg acaccgaagt ggactctgac aatgcggtct cccgtgtggt
    38161 ggcctccgta cctgccggcc catgggccgg ggccgtcctc tacagccagg acgccagact
    38221 cgcaggtcag ggaatggata tccactttga ccggatgatg tggcggggca tgtttctgaa
    38281 ggtgaatgca tatgccctga atgaacgaac aggaatgtca accgtggcca gcgacgtgaa
    38341 ccaccgctgg tttaagcaca tcgttctgcc gtccgtactt ggcggggtgg gcaatgtcgg
    38401 aagtctttac aaagacgcca acacacagat ccttcaggga aactacggca gtattaccgg
    38461 cagggtggga atgcctgatg gagaggtgct ggccggcgtc attgcaggtg gcatggccga
    38521 acggggcagc cagctgctga cccgccaggc agagagcgaa ccttaccgtc aggtgacagt
    38581 atcacggggc gaggtcgtct ccattctgtt tgttgagccg gtcatgagca acgatatcag
    38641 tacgccgtcc tccggcactg ccccttctga gtccccctcc tccggacgtc cgctcaccca
    38701 ggcggaggca gagcaccagg cctctgacag gatccgggcg gcggtcctcc gtaaacagaa
    38761 tgaaatgcag caacgctacg gcatacaaca ggaaagttat ccatgaaaaa cgaacatgaa
    38821 gatcccggca gctttggcga aacgacggca ccggcatccg gcaaatcctt cctgaaacgc
    38881 cagtatatgg gatacagcct gccggtctgg gccggctgtg ccctcatcat cctggtcgcc
    38941 atctggtaca agtttctgcg gacaccctca gagccccctg cagatgcagg ctttgctgtc
    39001 acaccgcccg ctgctgagag ctgggcagag cccatgacca tgacggagtc cgggatcgga
    39061 aacaacatgc ccgcaccgcc ggttacccca caggcagaca tgcagcaact tgcccgtgat
    39121 ctgaaagcag agctggacag acgggatgag gaaatccggc gcaccctgac catgctgaag
    39181 gacagtatca ccaccctcag taacgccatt cagaaggatg aggcctgggc acaggaaacc
    39241 cgtcagttgc ttgaggagac ccgtaagcag gtgcataccc tgcagatgca gggagcactg
    39301 ccagccccga ccgggaataa cagacagcct gcgaaaaccc tgccttcacc tctgcagggc
    39361 atgaaaatca tgtcggtgga gaaaggcatg gcctggatcc gctggcaggg cagtgcctgg
    39421 gcagtacggg aaggcgacag tctgggaaac atcaccatca cccggattga cccgtccagc
    39481 cgggcagtca tcacctctgc cggtacatta cgatgatgag cattgatgcc ctgacagcca
    39541 tagagaactt tgccagcggt attttcagtg caggcatgga gtttctcttt acctggggag
    39601 agctgctggg gattatcggc cttatcggtc atctgatgcg cgcccgggca gaaggacgtc
    39661 acagtatggg accgggaaaa tttattgccg gtatcttcat ctgcggcatg ctggtcagtc
    39721 tgccctcaat gatcaatgcc ggtggtaccc agatgggctt cagggcagat tctttcgccc
    39781 ccatcgcata cgtgcagccg ggctcctttg gtgccgctgc cggtgccgtc aatgccattc
    39841 tctcactggc aaaactggcc ggcgtcggtt ttgtcatgaa cggcatatcc atctggcgca
    39901 aatcactgct ggacgggcac accgccctga gcgccagtga atccatcagt aaagggaacg
    39961 tcaagtttgt ggccggcgtt ctgctggttt tcaacgaccg ggttatcgat gccactctgg
    40021 caagcctcgg tatcgccttc tgacccctgt attttttcag taaatcaatc tgttaataaa
    40081 ggagacacta tgtcacgact tcgtgccatg gcggcgcgtc tgcgcgctgc atctgtttat
    40141 gttcagttat gcctgatggc ctgccgtgac cggctggtac gcctgttact gctgcttccc
    40201 gtgatgctgc tgccccacac cgcaatggca aataccctct tcgatatgat taaaaccgtt
    40261 ggcgatggtg ccagaggctc cagcagttcg tttgtggcta tcggtcaggt tattggcctg
    40321 gtgctcttta tcggcggact gcttggcctc aaacaaatcg gtaaaaaacc cggtgcaggg
    40381 ctggcctgcc tgctgtcaat tctggtcggc gtctgccttg ccgtcgtacc ggaactgatg
    40441 ggcagcggac agaaaacgct cggtctttct ccggtttcaa tcagttaatc cgcaaaggca
    40501 ccgggaaacc ggtgccgtct tcacacaatg aggaggacca gaccatgtcc tgtgagctac
    40561 cccctttcac cctgctcaga acagagcacg gcaacgtgat tgcccgcgac aaccgggata
    40621 atcaggagtt tgtgtttgca gagtgttact ccaccgctat agcagcactg attctggctg
    40681 ccgtcaccct gtatacagag agaaaagcgt atgaaaaaga ccccgccact catcgccccg
    40741 gtccgggaac gatggctttt cccgctcccg caaccggaca cggtattcag cggtgaagcc
    40801 ctgttaccac cgccagtgct ctcaacagag ggacgctgcc cctgctgtcg caggaccgtc
    40861 acccaccgct tcattctgga agattcatgg ccattacagc agatggctga cacctgccgg
    40921 gatacgatcg tcctgctgga aaaaaacctc acccgggtga tgcgcctgaa aaaacgtccg
    40981 gtaccggaaa acgctgacga aaaaaagaaa cacacccgaa ccctgcagga tgcggagcgg
    41041 tccctggcac aggcccgcct gtcggccaga cgactggccc tgcgtcatgt ggaaaagtcg
    41101 caaatcgtca caacggatgc actcagcgaa aatgaaagtg acctgctgca gccggaaggc
    41161 ccgccctttc atctctgtgc cttctgccac gcctggcact gtctgaacgg ctacgctgcc
    41221 gcacagggag tgatggtctg gctccccgat ttgcatccgg cctcggtggt tgccctcaat
    41281 gcgcgggcac tgcaggagat tttcagcgat aaccgtcagc gggtcaggca gggacgggca
    41341 gtcctgaatg ccctggtaca gaaccgtctg gccgttgagg agaaattccg cacctggcgt
    41401 ccggcggatt ttgcagatgc cctgcgccgc tggccgccgg cacagcgtaa aacgttgagg
    41461 gagaaaatgg acggggtggc gctcattctg ctgccggatt cgtttcctga caaaaagtac
    41521 gtcatgtgac acacaccggt gccggtgacg ttttgccgga tatcagttct ccgcagatgc
    41581 attccggcac tttattatcc ggtcgctgac tgacgatatt ccgcctgacg gagatgaaaa
    41641 agagattgcc cggttcattc gccgccagca gtttatttat ccgggtgtaa tcacacagaa
    41701 atacgcccct gcccctcaac atattattta tcttctctgc tgttttttca tgccacatcc
    41761 cctgctgtgg catcaaaaaa tataagacct tgatgattca tccttaccgg agtgccccat
    41821 gccatgaata tcaaccgcat tatttttgcc attgaagact gtatcagtac cctgtcacgg
    41881 tataccgttt ccgcattctt ccccgattac tgtgatctgc acaccgtcat cggtctggac
    41941 cagcaggacc gcgaacgccg cccctggctg aaagccccct acattgccac cacaaaacag
    42001 gggtattacc tttccgtctt tgaaatcagc ggtgccctga aagaaatgga tgaaaacccg
    42061 gaccagacgg cccccgggac actggagtca ctgattgcag cactggctga gcgaatgaac
    42121 acggcctgga aaaattccgg ccacaaaatc agttttgttt tcgaacgcga tccggagcgt
    42181 ggaagggatg aaatcgaagc catgctctta ccccagcgaa aatccatcgc acgcacgggg
    42241 atcctgcttc aggacgtcct ggatgaaaaa gtcaccacac tcaccccctg gcttgtccgg
    42301 gagcgctgct ggctgaccgt ctggagctct gaagaactgc tgagccggac cgatcgcaag
    42361 aaccatcaga cccgtgtcag gaaactcgct gaacacgctc cgccagcccg ttttgcgcag
    42421 gatccctggc gctggacact ttccgccctc aaaatacggc atgacgcctt actggacacg
    42481 ctggagcagg ctctgacaca tgattctgat ggcctgctga ttcgcctgat ggatatccat
    42541 gaagtgggac gggaaatccg ccgccaactg gagcgcaaca gtacaccggc agtatggcag
    42601 cctcatctgc ccgaagatgc ccgtcctgcc ggctggcgac agggcgggga tacctctgta
    42661 ttccacgcac cgtcgctgaa tctgcagtta ttcaccacac agcctgaaac acacggcagc
    42721 ctgattcagg ccggtgagct ctggcacggt atggtcgcca ttaccctgcc ccctcagaat
    42781 ctgcggacat tcaaccagct ggtcagggat gtgccccgtg caatcccctg gcgtatccgg
    42841 atggatctga tgcccgacgg catgaaggcg ctgggggtca agaaaaccct gctgacctac
    42901 agcagcttta ttcctcctct ccggcccatg tatgactcgg taatgatgct gaatgacatc
    42961 aacaagcatg atccggtctg tatcatgacc attgttgcca ccacatgggg aaacagccgg
    43021 gaagtctgta cccgtaacca ggctctcctg aaatctgcgc tggaaggctg gggggtatgc
    43081 ggcaccacca caaccttcgg tgatccgaga cgtgcctggg tgaacacggt tctggcagcc
    43141 tcccacagct ccggcccgat ccccctttac ccgccccttt cacacgccct ttccctgttt
    43201 cccctgaaca gggcaggctc tgtctggcgg ggacagggaa atctgatgat gcacaccgaa
    43261 gacggctctg catgggaggt ggcgctggcg tcatcacaac agaacaagca cacggagctg
    43321 acacccggcg cacccggtct gggcaaatcg gtactcatca acgcactgtc ggagagccag
    43381 atagcctcag cccagaagaa tctccctttc attgcctata tcgacaaggg attcagtgca
    43441 cagggactgg tacagcttat ccgtgacagc ctgcctgagc agagaaagga cgaagccgtg
    43501 gggatcattc tgagtaatga cccggaccac acccgcaacc tgtttgatgt gatgtacggg
    43561 gccagaaaac ccgtcacacc ggaaaagaac ttcatggtct ccgtcctctg tgcattgtgt
    43621 gtggataccg gcaccggtca gccctgtaat cccggcgaca cccggcaaat catcagcagc
    43681 ctggtggacc tggccttccg ggaatatggc gagaataacc ccagacttta ccgcgcaggt
    43741 acggaacccc tggtggatct cgccctggaa gagagcggca tcgctgaaca acatgatgcc
    43801 ggatggtgga atgccgccac ctggtttgaa atcagggaca tgctgcatat tgcagggaat
    43861 atccccgccg cccagcgggc acactaccag gccatgcccc tgctagctga aatgtccgcc
    43921 ctgctgggac agccgtccat ccgggatgtg ttcggcaccg ttcagcgtga taactcggaa
    43981 gagcggttgc tggactacat ccgccgtgcc ctcgatcagg gacacagtga ctacccgatg
    44041 atgtccggct gtacccggtt tatgctcagc cccgacaccc gcgtggtcgc cgttgatctg
    44101 aacaatgtgg ccggggacaa gacacctgcc ggacgtctgc gcaccggtat catgtatctg
    44161 ctggccgggc agatcgccgg cggggatttt gtcctgccgc agtatcagga ggaaatacgg
    44221 aaaaatctcg acccacgcta ccatgaggtg attttccgcc gcattgaaca gcttgatcag
    44281 gaggtcaaga ccaaagtcta tgacgaactg cacaatgcaa aagggatcaa cttcatctgg
    44341 gaagccctgg acacccagga gctggagcag cgtaagttcg ggatacgcac ggtgctgagc
    44401 acccagctgc tgcagcatta tcctccggcc gtgctcaaat ccgccaacac cctgtggctg
    44461 ctgcgttacc gccctgacga aattccgttc ctgcgggata actttggcgt cccggaagtc
    44521 acactgcggc gcttcctgaa aatgccggaa ggggccgcac cggacggcag cggtgtcccg
    44581 gtactggctg tgttccgtgt aaaaaacggc acgctggccc ggatcctgaa attcaccctc
    44641 ggtccactgg agctgtgggc actgaactcc tctcctaaag acagcgccct gcgacgtgcg
    44701 ctgacgcagg aggtggggtc actgcgggca cgccagatcc tggccgaaca ctttccccgg
    44761 ggttcagcca cctcactgat agaacaccgc gcccgcacac atgacagtga aaacgtcatc
    44821 catgagctgg cagcagaact catccgtaag cagggatata acctctgatg tacagcattc
    44881 atatcacccc ccgctgccgg cgtcctgccc ggggacaggt cttcctgaaa acactgcgcg
    44941 tggacggtga cagtttcagc ttcaccctgc cgtccgcctg cttcacactg cgaccgtccc
    45001 ccaatccccg ggtcattttt gccagtgaca gacgggctga cggggagatg agtggtattc
    45061 gcctggccct gcccggagag acaggcacgc tgataacaac ggccacctgg caggttaacg
    45121 tgccggagcg accgcctttt gatatacagc atgtcattca ctggcttctg cccgcaccac
    45181 tcactgcgct gatgtctgac aacacctggc tgtggccgga tgtccccggc acaccggcac
    45241 tcacagccgg agactggccg gtcatggaaa gcgacacccc gacactgaaa cgcgtgaagc
    45301 gtcgtacctt cacgctcatc gataaccgta accgtcaggg atggatcacg cgccgcttcc
    45361 agtgtttccc gctggctccg ctgccgaaat cactcagtca tcgcgtacca gacatcctgt
    45421 atcccccgaa aacggagaaa aaagaatgca aagacaatct gcaaccacac tgcttgcact
    45481 ccttgtaggc tgtgcggtta tccctgccac ccatgcctac tctgtgaatg ttgtgtccag
    45541 tcagccggtc acccgacaga tcatgccgga gctggccacc atacagggca tcatggagga
    45601 aatggtccgt atgcagacgg ccaccggcac agccatcacc cagaactcag aaaaactggc
    45661 caccgtcatt gcccaggacg ggcaggccac ccgtcagcag atgatattca gtaatgaaac
    45721 acaccgcctc gaggaggcca ggaaaagctt cagcgtgccg gactccatct gcagtgaatc
    45781 cgcgtccggc attgcagcag aaagccgccg ggcagcagcg tctgcagcgg cacgtctgag
    45841 tcagggggga ggggtcagca gtaaacccat ccgggaacgt ctctcccggg ctgcggactc
    45901 cccggtccgc gaggcgtatg atagtgccgg catccacgcc ggttactgca cggaagcgga
    45961 gtatgcccgc tttggcggaa ctgatgtctg ccctgccgtg ggcgatctgc cgggcggaga
    46021 cagccaggta cgatcgctct atcagggcgc aggcacagca gataccccgg ccgctctcac
    46081 ctgggatcag aaacagattg atgccgcgac ggcttatatg aaaaacaccg ccagaccgtc
    46141 agcgggtcgt gccccgggga aaggagaagt gggcacacag accgggcgca cgtatgtggg
    46201 cctgcagaat gaatacaacg gcatcatcga tgccgcctct catccgcagc tgtcactgat
    46261 tgctgacagt acccctaatg aagccacccg tggggcactg actgaagccc tgcagtcacc
    46321 gtcagctgcg gcatattttg accggaccgc atcatcagaa gcccgcaccc gtggccacat
    46381 gagccagcgg gagtttgagg cctttgaagc cggcagacgg tatgccaaca ccgactggca
    46441 gcaggatctg cagggcatgg aaggcgataa ccttctccgt gagctgctgc gcaccaccgc
    46501 cctgctgaac tggcagatga acgatctgaa agagcagatt cgtcagggta acgttatcgc
    46561 cgggcagcaa cttgcgctgg ccgcacggca gtattacgga cagcgccttg gcgaactgtc
    46621 acaggccatg agccagggga gcgtaagatg aaagacgaca acagcatcga cgaaaaaaaa
    46681 ccggcccccc gtctccgccg catcggtgat atagggataa atctgttcgc accactggcc
    46741 gaaacccgct ggatgctgcg cagctgccgc catatgctgg accagcataa aacgcgcctg
    46801 aagcgcctca tgcccgaccg gcaaccggag agtccggtct gtatgacctg ggaggaagcg
    46861 gtcagggcca gcggactgac ggtcgacgaa ctggaccggc gtctgcgacg tcgcaggatg
    46921 ttctggcgga caggattctg gctgatgatg ataccggtac ttctgctgtg tggtatggcc
    46981 cttgcggcca ccgcactgcc ggtcatcacc ctgatacgta tcctggtctg cctgttactg
    47041 atgagtacca ttgcagcact gtgtgcctca aaagccctga ttgcgtcata ccgcctctgg
    47101 cagttgaatg aacgtcgggt cagcgaggac gaacagggca cattccccga ttacctgcag
    47161 gcccggcacg gctggaaaca ggccacatgg ctggccatta cccaccggca gtactgactg
    47221 tccggtctgc aatttctttt cctcattccg ggggctgatg cccgggcatc cccttcggtt
    47281 ccggagttac cgtgaaaatc atacccagag cactgagtgc aggtctggcg ctggctgccg
    47341 tacctgcaca ggctttcgtc acctatcagg acattgtgag tgccgccacc aatccggatg
    47401 acctgtcgcg ccaggcactg gtcaccattt ttggtgatgt ggtcacaaat cccctgacag
    47461 ccagcagcaa caccatcatt ggcaacctgt tcatggtgtt taacgcaatt atcgccacga
    47521 tggggatagt ctggtttctg tttattggta tccgtcatgc tgtccgcacg ggacaccagg
    47581 gcagtgtctt taacaacgga cgggacgtcg tcggcatact cagtgtcgtg agtggatttt
    47641 tgatgattgt gcccaccgct tccggctgga gtctggcgca actggtcatg ctgtggggcg
    47701 catccatcat gggcatcggt tccgccaata tcatggttca gacagcggca gaaaacattg
    47761 ctgaaggcta ttcaatgacc gtacagccag ttcatgtctc cacccgcacg gcagcacggg
    47821 gaattctgga gatggaactg tgcaaacacg ccatgaatac gggcctcaat gactttaacc
    47881 gtaccgcacg ttcatccacg ccgcccatga gcgaatcctc acggacagag aatggcagat
    47941 ataccgtcac cgtctcaaat ggcagcgcca cctgcgggag tgtgagtctc tccgtgaaag
    48001 gaaacggcag taacaagcag tccgcgctgg cccatttttt caatcccttc attgaaacgg
    48061 aatatcaggg ggttctgacg gcacagcgct ccgccatgga taccatgctc cgtgacctgg
    48121 acagcgcggc gcaggaattt gtgtcggcat accggacccg gcgcgacacg ggtaacggca
    48181 acctgccgga tattgaaagc cgtattcaga aggcagccaa ccggtatgaa gaaacggtgc
    48241 agaaggcgtt gccggcagat aacgcagaaa ctgagcgcag ggagcagctg aaatcctacc
    48301 tgaccaccta cggctgggtt gccctgggtg cctggtacca gacctttgcc accgccaacc
    48361 agcgactgga agacctggcc aaccgcgccc ctaccgtgag tggcgtctcg tcactgggcg
    48421 aagccgggga tacggaattc ctgaaggaag tcaagggggc ttaccaggca cagttgcaga
    48481 attcaccata tactcctgca ctgggaacaa acacttcatc aactgaatac agaacagcgg
    48541 aagccgctga acctaaaagc ataataatga aagtatggtc gggtgaaaat tttgcagaca
    48601 aaattgcgac atccttatct ggaacaggaa catcctcgga ccaactaaat ccactgataa
    48661 aaatgaaaaa tatcggtgat tacactatgg gtattactga aagcatatgg gcgggttata
    48721 ccaccgtcag ggtactttcc acagccggca gcaactccgt ttttggtcga ctggtcaatg
    48781 ccgtcagcgg attatctgac gccgcaaagg cttttctgga agcatccgca ccaccactct
    48841 acttcctgct gtttttgctg ttctgtgccg gcttttcgtt atccatttac ctgccgttta
    48901 tcccgtttat cttctggatg accggcatcg gcaactggat agtcagcgta ctgattggat
    48961 gtgctgctgg ccccctgtgg gccgccaccc atctgggaac aagccaggac aggggaagcc
    49021 gggctgctta cggttacatc tacctgatag acagcatgat cagaccgctg ctgatggtgt
    49081 tcggattctt ttttgcatcg gtcgccatcg tggccggcgg caccattctg aatgcgttgt
    49141 ttggcgcggc tctggttaat gtgcaggcgg actcccttac ggggttattc agcatcgctg
    49201 gatttttgct gatttatgcc cgagtctgta ccacactggt tgcagccgtt tttgcgcttc
    49261 aggcttatct gcctgaccat gtaattaatt ttctgggcgg acgtgacggt gttaatatgc
    49321 tggggaatat ggtttcttca gtcaaagata ttattgccgg aggtaatacc aatatgcgca
    49381 gaacacctgg cgttcgtgaa gaaaaactga aaaatgttaa tggagatgat aaagacggaa
    49441 taaaacaata aataaaaata tcgaaaaacc aataaaacac tattgccata ttcacctgaa
    49501 gagattaaaa tatattcagg tgaatatggg ggtttattat gagtacaaca aaaagcagat
    49561 atccttcctg gtggccatat tattaccttg ttcgcaatgc gtatttcata tttggcatac
    49621 catgcttatt attttatgct tttttcggaa cactaattgt tcatgattca gccttatctc
    49681 aaacaatgga ttttattttt gcatatggat gctgggcatt aataattctc ccattactct
    49741 ggctatatct gagaaccaga gcgaaaaaaa agaaagtcca gcaggttgtt cagcagataa
    49801 aagaaagctg tcactttgct ccaaccagcg attatgagca actgtcattc tcgaagagtt
    49861 gttatttcgg gatcgacatt aaaaacggca ccatgctgta tgtgcgcatt tatccgaaca
    49921 atgtgatgga tgtgattggt ctggatattc acaactttac ccgcacggtt gctgaagacg
    49981 gcaagctgga aatccacact acatatgtga gtctgccgat gatcccactg gaaatctccg
    50041 gcatctcatc aaggacgctg gccaacacca tgcacaccat ggctgcacgt ggatatgagt
    50101 acaaggaacg tttcccacag atgatccggt atcgggttaa agagtgggaa aaagttgccg
    50161 gtgttcctgt tgcagaagta ttttgaggta gctatcaact gttagacgat aatccgtcag
    50221 ccggttttct ctgaaccggc tttttctttt caggcagaat gcctgtctgc cgtctatctc
    50281 aggacgccgc atggagtctc tatgttttcc ccacaaattc ttagcactgt cttgcccact
    50341 gtagacaaaa tcaatacaat tctgcatatc agttatctat aaaagataac aatatcatta
    50401 taaataatta tcataaccac acactcaagg cagtacctag aaaacataaa gataaaatca
    50461 tgtctgaatc ccgcagcaat tttccagcac gacatggtac tccatggaat actgaagagt
    50521 tacagcgtct tctcgtatcc atagcggagg gcgtctcgct aaaaaaactt actcaaacac
    50581 atcaacgatc gtccggggga atacgtggag caatcaaacg cctgcttcct tctgagtttc
    50641 ttgagttcaa cgatataaac acggtgaatg atctcagcaa ttacttcaac aagattcctc
    50701 gccaagaaca caatagtatc atcaataatt taatatcatt gagcactgca aaaacaattg
    50761 ctcatgataa agttgaaata caaaagaata gttttaaaca aaatacccca ctccccctct
    50821 gccaacccaa tacagatttt aaagattata tagccgatga acatgatgtc attatgctaa
    50881 ttcattctgc tatcgaaagt ttatctaata aacgacatca atatattctt ctaatgcgac
    50941 tcggcacaga tgattcacct cattctctgg cagatattgc cgtcaaatta ggaatttccc
    51001 gtgaaagggt aagacaactt caggaacgag cccttggctt actagctgct aaatctcgct
    51061 atgaagggac acctggagct tgccttaaag cccttattaa ctcaataggt aattcaccat
    51121 atgatatagc agtatggatt tatcgtattg ttcatcggga tttcgttaca tcctctgagt
    51181 tagccgccaa attcatcatt tttgccgcag gcattcctaa agcaagacga gatgaaataa
    51241 aaaattcgct ctcattaata tcacaattac aaaagcagaa gttacgtgaa cgaagagcag
    51301 aattagcaca cgctcacgct caggaaagag cccgaatcaa gaaagaaaga gttgagttca
    51361 tattcagtaa atggctagac aatacacatt ggccaaaata tatcgcacct cctccccccg
    51421 tagaacagct atgttcacag agagcggtta atgatactga catatctgga ttcttttact
    51481 ctcaaaaatt aggtcgtaca gtccagtacg aatcagggct tgagtttaaa ataatgaatc
    51541 tattagaatg ttgcgatcag gttcttttct atcaagaaca accattttct attccatatt
    51601 gttttaaaaa caaatcaaaa aaatactatc cagatttact cattgcgaca gttgatggtc
    51661 ggtgcctact tatggaagtg aagccaacag accgaatgac cttgagtctc acgagaataa
    51721 aaagtaatgc aggtcgcaaa tgggcacata ctcgaggatg gggatggttg gtcattagtg
    51781 ataggttctc gttacgtcag ctggaagaac atgccatctc aacaaacaca tggaaactta
    51841 tagaggctga actgaaaaca tcaaggattt tagcatggcg tgaaatgatg gagttacgta
    51901 cacgatacga gattcatcgt ttagatctta ttgcatatat cattcagtct ggtgctgaag
    51961 tggataactt ttatcgaatt accccgaaaa tatctaataa tacatctaac cagagacaac
    52021 caaatgagag cgactgtcat aattgatgac gcacaggacg gaaaaaataa tagctgccgg
    52081 acagcagcat ccagcagtcg tccggtaaaa aagacaaaca aggcgcattg aggtcagcct
    52141 tcgcaacaat ccggcggcag atgtcctgcc gccgtgttgc ccgttaacgt ttaacttcgt
    52201 aggctaacgt tgccacaaac accgtatttc cctgctcgat acgaaatccg caaagcgaat
    52261 ccctcaccat ccagacaaaa cacagtatcg tcacacagac gacgattaac atcattaaaa
    52321 acgttcgttg tggcatcgca gcccctccat tgcctttcgg ccgtcatcgg gctactataa
    52381 tgttgtccag gcattacagt gggccctggg ttgattgata aaaaaatcgc ctggggcttt
    52441 ctgctacatg ccgtccacaa aacgctttcg gcatacagcc tcaagcgccc gccgctattc
    52501 taatcgtttt cctgtccgcc acaatccttt tcatgccacc acgtattttg tgtcagggac
    52561 agaaataaag ccggaaagtg actacgtgtg cacacatctg ttttccgggc tgcaggcatc
    52621 gtgttatttt ctgaaatgca aaataataaa aaacaaggac acatcatgaa ggatgaaata
    52681 cacagtacca gcattaaccc ggatgttgtt gagcagtttc tggcccttat gaatacacat
    52741 tgtaaatcct cagaagaggc gctcaccatg ctgagtacgc tttgttacaa cctgatgatg
    52801 acatgcggta tccattccat ggacagccag atgctcaatg ggcatgggat atccatgcaa
    52861 cttcagggga ttgatattgc cgaaaagcca ggcaagaata ttcactaacc tgaacagggc
    52921 ctgaaaacgc tgtcttttca ggcctctgca ggcaaaaact gatgacaaca aaatttctgc
    52981 gtttgtaccc caccgatgaa aaagacaaaa actcacaccg ggcttctcat cattaaagat
    53041 aaaacccgac gtgtcagcct ctacgaaacg ccaacggcat ggtgtatcag ggggcaggag
    53101 tgctacagca aaagcactgg acgccgctgt ggcagccacg acagtctgag cagactgcgc
    53161 ctggacagca ttaagcctgt tgaatgagtg gtggcaccat gaaagataac aacacaaccg
    53221 ccaccccaga aacctggaaa atgcgttttc tggccaacgg gacatccggc acatggtttt
    53281 acactgacga cattgcagtc actgacgcgt ggcttcgcgc gcacccggat gatgtggagg
    53341 ttatccctct ctgccggata cagccccgcc agcattccca tgaacaacat attgaagaac
    53401 tccgcgcagc cctggcccgg atgctccata tgcatcatat gatgctggaa accacacatt
    53461 accgggaaaa tatcaccgat gcccgttgta tccgtgaaat gcatgaagcg ccattacagg
    53521 cgctcaatat tctgtccaga tacccgcata aaaaggacga ggtgtgacgc atgtggctac
    53581 tgattttctg gctttcatcg ccctttaatg tctcttccgg tgatgcccgt cagcccacac
    53641 tggcggtgca cagccaggcc tttcataccg aacgggcctg ccgcgatgca ttgcgcgcca
    53701 ttaaaaagga gaatgttggc gaattttctc tgcggggcgt ttgtgtttct gacggaaata
    53761 cgaaggagga ggctgagagc tcatgacgga tattactgac agacatacac ttcgccgtat
    53821 gtcctggtcg gaactcatca cggcaggaca ggaagcagaa tttcagcatg attacgagcg
    53881 tgccagaata gtgtggtcat tcgccttaca tgttgcaaca accacgatta acaaaaatct
    53941 ggccatagct catattcgac gctgcgatac tttgttgcat aaatcaaagg ctgtacccgg
    54001 aaacaacccg tgtggttccg gtcagcgagt ctgagacctc aacatcccgg gtgatgattg
    54061 tcattccgga ctgacgcagc cgctgcacat ctgcggcaat acgttgaata ccaagccaga
    54121 acagaccatc cagcgcggtg acaattaacc cgctttccag cgccagtttc agccgtgctg
    54181 tcggggcttt aacctgcccg gctatatcag tgaaacaacg cgtcgtcatg ccatagctgt
    54241 ccgcccgccg tattcgcgtc gcaagactgt aactgaagct atccggcacg ccggataagt
    54301 cattctgcag actgtataca catccccgct gccgctcatt gaatacaact tttttctggc
    54361 tgagctgcca ttcccgccgc acgctgtcaa tcatctgcgg tgtccgggtt aacagcagac
    54421 gaaacggcag ttcaaaactg gtcacataat caacattaag taaggcaatc cgcagccgct
    54481 ccctggctcc tgctccggta tcacggcatt cacgcatgac atcttcccac tggcgcatca
    54541 gtcgcggtga ctcattcctg gccctgaact gatatttctc aatctgataa tcaatatgtt
    54601 cctgcatacc gtcaccggat atctgttgcc gtatcatcac ggatatcagc gtactggaat
    54661 tcatgccctg cgtgaagtat cacactgcca aaaaaaacac acctgaacgt tattatttcg
    54721 caatattaat tgcacctgtt ttcataaata aaaaggagac gatgaaatga taaaatatat
    54781 attttcactc accattattt tttccgcctc tctctgtgcc gcacagattc aggggcacgt
    54841 catccgcgtt cttgatggtg atacgctgga aatattacag gacaaaaagc ctgtcagggt
    54901 ccgtctggct aatattgatg caccggaaaa gaaacagccc tttggacgct ggtcagccac
    54961 tcagctgaag gacctggtgg ccggaaagcc cgtaaccgtg caatacgcgc agacagacag
    55021 gtatggccgt attctcggcc gggtctatac cgctgacggc acagaagcaa atcgctggca
    55081 ggtccgtaac ggcgctgcct gggtgtaccc ggactacaat aatgactata ctctcccgcc
    55141 gctccagcgt gatgcccgcg ccctgaagcg gggattatgg gcagataaaa atccggtacc
    55201 accatggacc tggagaaaac agaataagaa ataattattt gctgatatga agaaaaaaac
    55261 catttcaaag aaaataaatg gcaaaaatac agcatggaaa aaattgccat ttttaccgcc
    55321 gcgttatttt taatatttgc tgtctgctgg tcgtaataag cagaataaca gaaaggtgta
    55381 aaaatgaacc tggttgatgc atttgtcact aaagtgatat ccggaccgta tgaagaatac
    55441 ggaaaatggt gggtggatgt agaatatatt tcctggggag ttccgggtaa aagccggctc
    55501 atgtttgact cacgggaaca ggcacaggaa gtgcaggaag gttttcgatt tttaacctga
    55561 gcatattttg ttaaagggca gaaggcggtg accctctgcc cttaagtacg tttccgggat
    55621 tatcagtcaa ccagttcccg gatcagttcc aggattgcta tcactgcgtt agccacagca
    55681 acaaccgccc tgagaacgat gattaacagg ttaatcatcc gcttctccat caatggagaa
    55741 agaacaccgt ggtttgccct tacattgcct gtcgccacgt tttctatagt gtcagttata
    55801 gacagttctt ctccggccag agcaccaaaa ccagcaccac ctgatgtttt ggccaggact
    55861 tgcaacctgt tcatcccggt gaacgatatg ccggaaagaa cgtgcctcaa agctgacaca
    55921 ccgagtatat acttatactg tgcttgcaga aatattattt cagccgtatt atttcatagt
    55981 cagttatagg cagttatctt ccggtcaggg ctaccaactt cgatccggaa tcgcaaaaaa
    56041 accggcctca ccagtcggtt tttttgcgtc tgtacctcct ccccggacag cagttctctc
    56101 actcccgttg aatatttcct gttgaatttt acgaaaatac ggcaaatggg agatatcgaa
    56161 tatatttact tgggtgtagt cggtaaaaac cagctcatgt ttgactcaca gaaacaggct
    56221 ctggaagtac agaaagtaat ctgtccgtag attattaaag ggcagagggc ggtgaccctc
    56281 tgcccttaag tacgtttccg ggattatcag tcaaccagtt cccggatcag ttccaggatt
    56341 gctatcactg cgttagccac agcaacaacc gccctgagaa cgatgattaa caggttaatc
    56401 atccgtttct ccatcaatgg aggaaatcac tgttgtttac tcttacattg cctgtcacaa
    56461 cagctctttc attgttagct gaaaagagac ttcctccggg ccagagccac ccactctccc
    56521 tgggatcgca aaaaagccgg cctcaccagt cggctttttt gtgcctgtct ctcaccacct
    56581 tctgctgttc ctgtgaaaat gccctgctga attcagccct gcccagtcat taccggcgat
    56641 tgttcgcatt aattccactc agcctcatcc cgaaatttat ctatttatcg atggaggaat
    56701 ccccgtgtct taccaacaga cgtctgccgc cgaagatcca atggctatat ggtatatcgt
    56761 cggtgccata tgcctgctct ttgcgattat catctggcgc tttttacctg aaatcgtctt
    56821 cgcctcctgc ctgatcctgc acacactgtg gggcatgatt gactgggggc cctttcacaa
    56881 ctttgccgcc ccccgttaca acctgctggc catcactgct aacaacgcgg caaccatcac
    56941 tttcggccag tggctggatg ttatgtcccg gacagtgggg atcctctggt tgattctgct
    57001 tcccatgaca ttcggctttc tgtggatgtg gtttcaccat ccggcccagc cccgttttac
    57061 ccgcaggcca ctgaatatcc acacactgcc ccatattttt tcagctctgt caccggcgat
    57121 cgcccccgtt ctggctgatg gagacaacaa cagactcttc cacggacaaa aacgtcccga
    57181 gcgccgtgtc gcactaaccc ccgaagtctt tgtggagcag aacaacctta tccgcaacat
    57241 gcagctcgat gttgcgtcca cacgccagtg cttcatggcg caactggggc aaccactgac
    57301 cagctggaaa gacatggccc cacatgaaaa agcgctcttt gccatctttg gcctgcagtt
    57361 cttcctcggt gaccggaaag cagcggtagc cctgatgaac aacctcaatc tctcctgcag
    57421 gctcaaaagt aaacgggacc aggggcgttt cagcacgccg gtgtattcac tggccagaaa
    57481 cgcctttatc cgggtcatca aaacagaagg cgcacaacag tggctcaggc agcaccgtta
    57541 cgttcgcagc ggtctggtct ggctttacgc acatgatttg cgcctgacgc ctccgaactg
    57601 gctgtggctc aagggcgttg accggacgct cttctatgcc cttcaccggg caaacaccac
    57661 aaaaggcttc atcgaagggg ccggcgtggt ggccgttgcc cgggcagaaa atgaagccag
    57721 ccgtctcggc ctcccctgcc cggaaccctg tgtggaagag gccattgaag ggcttcgacg
    57781 ggacatgctg gggctgggcc tgatatggga cgaacctcag ccagatcgcg atcgtaaacg
    57841 tcagatcagg acccgctggt cactcacgga tgacgtgata ccccgccgcc atgataacga
    57901 cgaagacact gacactggtg aaaccaccga aaccaccgaa accaccgaaa ccaccgaaac
    57961 caccgaaacc accgaaaaac agcatcccgc cgacaaggaa aaagaacaat gaccatccac
    58021 tacactgcaa cactgaataa taaacacctg tccctgatcg ccctgtaccc tgacggtaat
    58081 cgggaggtga ttgccatcct gccagtcaca gaactgggcg cactgattaa ccgtaataac
    58141 aggctgatgc acctgtcctt cagttcccct tttcgcgaaa taaccgaaac ggtgcttgtt
    58201 gataccgttg atgcagaacg tcccggacat cacatcggca gaatgctctc agcgattgat
    58261 gcctgcctgg atgccgaata tgccggaaaa ggaaaacatc cctcccggaa aaatggcttc
    58321 tggcggacac tgatgtgtgg ctttatcgcc ggaattgctc tgccggcgac agttgcaggt
    58381 taccactgga tgaaggcaga gcccccttcc acgctggctt accctacatc tcaggaatgg
    58441 agagctgaac cagtaaccga cacagagaca ccacgcccac atacggaaca tcatgttaac
    58501 agcatcccgg cccgcccggc tcccacacag caggaacctt cttctgcccc cgccccgacg
    58561 gcagtgacgg aagaacaaca cgttcaggcc gctgaggccc gccgtaatct ggccacagta
    58621 cttaaacgca acgcagaccg ggggatgttc accattaacc tgtccactgg tcatgaacgc
    58681 acgctgtacg ccttccttga ccccgcatgc atcaactgcc gtctgctgga acccgcgctt
    58741 aagcaactgg cccgggaatt cagcgtggtg atttatccgg tctccgtgat tggcggggag
    58801 tccagtacaa agcggactgc tccgctgctg tgtgaaccgg actcacaaaa acgggcgacc
    58861 ggatggcatc agctgtattc cgctgacagt ggtatggccc ccaccggcga tgcaccggcc
    58921 gcacaggatg gacagtgtct gaccgccgca cgtgctgcca tcgatgtcaa taaccaggct
    58981 ttccgccagt tcggttttgg cggaaccccc tgggtgttat cagataccgg ctggcacctg
    59041 ccgtccggca tcctgaaaga cgccacttcc gttcgcctct tcctgaaagc aacagatagc
    59101 gaggagtatc acaatgagcc agcaccacgt taatcctgac ctcatacacc gcacggcctg
    59161 gggaaacccg ttgtggaatg cactgcataa cctgaacatc actggtctgt gtctggccgg
    59221 cagtatcatt acggcgctga tatggccact ggcccttcct gtctgcctgc tgttcaccct
    59281 ggtcacctcc gtgatattca tgctgcaacg ctggcgatgc cccctgcgta tgcccatgac
    59341 actcagcctt gatgacccct cccaggacag aaaggtcagg agaagcctct tcagcttctg
    59401 gcccaccctg tttcagtatg aagcggatga aacattcccc gcccggggga ttttttatgt
    59461 cggttaccgc cgtatcaacg atatcggtcg tgagctctgg ctgagcatgg atgacctcac
    59521 ccggcatgtc atgttttttg ccaccaccgg tggcggtaaa acagaaacca cctttgcctg
    59581 gctgctcaac ccgctctgct ggggaagagg ctttaccttt gttgacggaa aggcacagaa
    59641 cgataccacg aggacaatct ggtatctctc ccgacgtttt ggcagggagg atgatatcga
    59701 agtcatcaac ttcatgaacg gcggaaaatc ccgcagtgaa atcattcaga gcggcgaaaa
    59761 atcccgccca cagtcaaaca cctggaaccc gttcgcattc agcaccgaag ccttcaccgc
    59821 cgaaaccatg cagtccatgc tgccccagaa cgttcagggc ggtgaatggc agtcacgggc
    59881 gatagccatg aacaaggccc tggtctttgg cacaaaattc tggtgcgtcc gggaaggtaa
    59941 aaccatgtcc ctgcagatgt tacgggaaca tatgacgctg gaagggatgg caaaactcta
    60001 ttgccgtggc cttgatgacc agtggccgga agaagctatc gcaccactgc gaaactacct
    60061 gcaggatgtc ccgggattcg atatgtcact ggtgagaacg ccgtcagcct ggactgagga
    60121 gccccgtaaa cagcatgctt atctctccgg tcagttctcg gaaacgttca caacctttgc
    60181 cgaaaccttc ggggacgtct ttgcggcaga tgccggcgac atcgatattc gcgacagtat
    60241 tcacagcgac cgcattctga ttgtgatgat ccctgcactg gacacatcgg ctcataccac
    60301 gtcggcactc ggtcgtatgt tcgtcaccca gcagagcatg ctcctcgccc gtgacctggg
    60361 ctaccggctg gaaggcacag atgcacagac cctggaagtc aaaaaataca aaggcagctt
    60421 cccctatatc tgtatccttg acgaagtcgg tgcctactac acagaccgca ttgctgtgga
    60481 ggccacccag gtacgttcac tggagttttc cctgataatg acggcgcagg atcaggagcg
    60541 aattgaaggg cagacctccg ccaccaacac cgccacactg atgcagaacg ccggcaccaa
    60601 attcgccgga cgcattgtca gtgacgacaa aacggcaaga acggtgaaaa atgccgccgg
    60661 tgaagaggca cgggccagaa tgggcagcct gcagcgccac gatggtgtga tgggtgagtc
    60721 ctgggtggac ggtaaccaga tcaccatcca gatggaaagc aaaatcgacg tgcaggattt
    60781 gatcagactg aatgctggtg agttctttac cgttttccag ggggatgtgg tgccgtcagc
    60841 ctctgtgtat attccggaca gcgagaaaag ttgcgacagt gatccggtgg tcatcaaccg
    60901 ctatatttcc gttgaagccc cccggctgga gcgtctccgc cgtctcgtcc ccaggacagt
    60961 gcagcgcagg ctccccacac cggagcatgt cagcagcatc atcggtgtac tgacagcaaa
    61021 accatcccgt aaacgtcgta aaaaccgtac agagccttac cgtatcattg atacattcca
    61081 gcgccagttg gcaacagcgc agacatcact ggatctgctt ccgcaatatg atacagatat
    61141 cgaatcccgg gcaaacgaac tgtggaaaaa ggctgtgcac actatcaata acacaaccag
    61201 ggaagaacgc cgggtatgtt atatcacact gaatcgtccg gaagaggaac actccggtcc
    61261 tgaagatata ccttcagtga aggcgattct gaatactctg ctccccctgg agatgctgtt
    61321 acctgtaccg gatctaactg catccccgcc ccataaaaaa aatgtcgctc agacaccatc
    61381 aggtggaaaa caagagagtc gtaaaaaaag attctgataa cacctgtatg gctgatttta
    61441 ataacaacct ttacgcgatt attaatctcc tccgctcacc atagtaagcg gaggattcag
    61501 gatttcaatc catcaccaga ggtacaataa atgtgtatta gtagcccgga aacaaactca
    61561 tggtctgtta tttatcgtaa aaattccgga gaagatatca atatcacttc cctgacgttt
    61621 aaaaatagtc ttctggcagc ccggacactg atggtgcccg aaaactatat gatatgcata
    61681 ctcagaaacg gggagagggt cagacgatgg gacagagaaa tacttgcagg ctcaaacaga
    61741 tggtacaagt gttctcccga taatttcgaa atactgggta agctccccat aataaataaa
    61801 gtcacaaaac tgataaaatc ataatctacg gattttaatc agttaagcta aatcaggatg
    61861 aacgctttga ttatgaatta tacatgaaaa caaatatggg cagcacaaaa aatggtgcca
    61921 gggttcccca cgttcgtaat agattcactt ctatggatat tggtatgaat tcccagatcc
    61981 cactctattg taaacaagac atttttatct tttatattca atggcttatt ttcctgctaa
    62041 ttggtaatac catgaaaaat accatgctca gaaaaggctt aacaatattt tgaaaaattg
    62101 cctactgagc gctgccgcac agctccatag gccgctttcc tggctttgct tccagatgta
    62161 tgctattctg ctcctgcagc taatggatca ccgcaaacag gttactcgcc tggggattcc
    62221 ctttcgaccc gagcatccgt atgagactca tgctcgatta ttattattat agaagccccc
    62281 atgaataaat cgctcatcat tttcggcatc gtcaacataa cctcggacag tttctccgat
    62341 ggaggccggt atctggcgcc agacgcagcc attgcgcagg cgcgtaagct gatggccgag
    62401 ggggcagatg tgatcgacct cggtccggca tccagcaatc ccgacgccgc gcctgtttcg
    62461 tccgacacag aaatcgcgcg tatcgcgccg gtgctggacg cgctcaaggc agatggcatt
    62521 cccgtctcgc tcgacagtta tcaacccgcg acgcaagcct atgccttgtc gcgtggtgtg
    62581 gcctatctca atgatattcg cggttttcca gacgctgcgt tctatccgca attggcgaaa
    62641 tcatctgcca aactcgtcgt tatgcattcg gtgcaagacg ggcaggcaga tcggcgcgag
    62701 gcacccgctg gcgacatcat ggatcacatt gcggcgttct ttgacgcgcg catcgcggcg
    62761 ctgacgggtg ccggtatcaa acgcaaccgc cttgtccttg atcccggcat ggggtttttt
    62821 ctgggggctg ctcccgaaac ctcgctctcg gtgctggcgc ggttcgatga attgcggctg
    62881 cgcttcgatt tgccggtgct tctgtctgtt tcgcgcaaat cctttctgcg cgcgctcaca
    62941 ggccgtggtc cgggggatgt cggggccgcg acactcgctg cagagcttgc cgccgccgca
    63001 ggtggagctg acttcatccg cacacacgag ccgcgcccct tgcgcgacgg gctggcggta
    63061 ttggcggcgc tgaaagaaac cgcaagaatt cgttaactgc acattcggga tatttctcta
    63121 tattcgcgct tcatcagaaa actgaaggaa cctccattga atcgaactaa tatttttttt
    63181 ggtgaatcgc attctgactg gttgcctgtc agaggcggag aatctggtga ttttgttttt
    63241 cgacgtggtg acgggcatgc cttcgcgaaa atcgcacctg cttcccgccg cggtgagctc
    63301 gctggagagc gtgaccgcct catttggctc aaaggtcgag gtgtggcttg ccccgaggtc
    63361 atcaactggc aggaggaaca ggagggtgca tgcttggtga taacggcaat tccgggagta
    63421 ccggcggctg atctgtctgg agcggatttg ctcaaagcgt ggccgtcaat ggggcagcaa
    63481 cttggcgctg ttcacagcct atcggttgat caatgtccgt ttgagcgcag gctgtcgcga
    63541 atgttcggac gcgccgttga tgtggtgtcc cgcaatgccg tcaatcccga cttcttaccg
    63601 gacgaggaca agagtacgcc gcagctcgat cttttggctc gtgtcgaacg agagctaccg
    63661 gtgcggctcg accaagagcg caccgatatg gttgtttgcc atggtgatcc ctgcatgccg
    63721 aacttcatgg tggaccctaa aactcttcaa tgcacgggtc tgatcgacct tgggcggctc
    63781 ggaacagcag atcgctatgc cgatttggca ctcatgattg ctaacgccga agagaactgg
    63841 gcagcgccag atgaagcaga gcgcgccttc gctgtcctat tcaatgtatt ggggatcgaa
    63901 gcccccgacc gcgaacgcct tgccttctat ctgcgattgg accctctgac ttggggttga
    63961 tgttcatgcc gcctgttttt cctgctcatt ggcacgtttc gcaacctgtt ctcattgcgg
    64021 acaccttttc cagcctcgtt tggaaagttt cattgccaga cgggactcct gcaatcgtca
    64081 agggattgaa acctatagaa gacattgctg atgaactgcg cggggccgac tatctggtat
    64141 ggcgcaatgg gaggggagca gtccggttgc tcggtcgtga gaacaatctg atgttgctcg
    64201 aatatgccgg ggagcgaatg ctctctcaca tcgttgccga gcacggcgac taccaggcga
    64261 ccgaaattgc agcggaacta atggcgaagc tgtatgccgc atctgaggaa cccctgcctt
    64321 ctgcccttct cccgatccgg gatcgctttg cagctttgtt tcagcgggcg cgcgatgatc
    64381 aaaacgcagg ttgtcaaact gactacgtcc acgcggcgat tatagccgat caaatgatga
    64441 gcaatgcctc ggaactgcgt gggctacatg gcgatctgca tcatgaaaac atcatgttct
    64501 ccagtcgcgg ctggctggtg atagatcccg tcggtctggt cggtgaagtg ggctttggcg
    64561 ccgccaatat gttctacgat ccggctgaca gagacgacct ttgtctcgat cctagacgca
    64621 ttgcacagat ggcggacgca ttctctcgtg cgctggacgt cgatccgcgt cgcctgctcg
    64681 accaggcgta cgcttatggg tgcctttccg cagcttggaa cgcggatgga gaagaggagc
    64741 aacgcgatct agctatcgcg gccgcgatca agcaggtgcg acagacgtca tactagatat
    64801 caagcgactt ctcctatccc ctgggaacac atcaatctca ccggagaata tcgctggcca
    64861 aagccttagc gtaggattcc gccccttccc gcaaacgacc ccaaacagga aacgcagctg
    64921 aaacgggaag ctcaacaccc actgacgcat gggttgttca ggcagtactt catcaaccag
    64981 caaggcggca ctttcggcca tccgccgcgc cccacagctc gggcagaaac cgcgacgctt
    65041 acagctgaaa gcgaccaggt gctcggcgtg gcaagactcg cagcgaaccc gtagaaagcc
    65101 atgctccagc cgcccgcatt ggagaaattc ttcaaattcc cgttgcacat agcccggcaa
    65161 ttcctttccc tgctctgcca taagcgcagc gaatgccggg taatactcgt caacgatctg
    65221 atagagaagg gtttgctcgg gtcggtggct ctggtaacga ccagtatccc gatcccggct
    65281 ggccgtcctg gccgccacat gaggcatgtt ccgcgtcctt gcaatactgt gtttacatac
    65341 agtctatcgc ttagcggaaa gttcttttac cctcagccga aatgcctgcc gttgctagac
    65401 attgccagcc agtgcccgtc actcctgcca gacatgctga ttaattttgc ccttattaac
    65461 atcacagaca gaaagaacga gggtaccaat accattgatg gtaactggca ggccgatgaa
    65521 ggacgccgtt accgggataa tgtaagaatc tatttttgag tatgtcaaca taacctgagg
    65581 caaagtacct caggttccag tgtagtaatc agccaacctg acagtcttct gttagcccta
    65641 cagatatgtc gtggagctaa ccgcgcatgt caaagccgga tttttgaaaa tctctataaa
    65701 cttccggatt gttgaaggcc gggaatttag ctttatgggc tgctgacttt atcgcttcgc
    65761 aataggcttt atcgccatta ctggttgata tttttaacgc cgtgccatcc cgggagaatt
    65821 ccatatgcaa cctgcatttt ttccttttcc atttttgcgg atcatcaagt ttagcattaa
    65881 ttgcatccct gattccccgt gcttgctccc cccattcatc ctgatcatcc caacgtcctg
    65941 aactgcaact acccagggct gtagttttat ggcaatttga aggatgtaat ggcgcacatc
    66001 ccgccacaaa gctgactaaa atagccagca taacgatttt ctttaccccc atatattgct
    66061 cctcaatcca tatagatctc agtaccatag ctctgtcaga gcaaagaaaa tcttaactaa
    66121 tacaagttaa gatttttaat atccactcct catccctaca ttccaggtga tggtggcgtc
    66181 cactcattat cctgaactgg agcataagat gcgccatgcc ccccggatat gcttttctgt
    66241 tgcctgatat attcgtggta gtactgttca ctacgctgac gatgtaaatc cacatccggg
    66301 cggctgatta caaagtgtcc acttccggtt attccagctg cattatgcca ggccaccatt
    66361 tccgcaaata catcattaaa ctgttgatat gagccttccg gttcaaaacc cattcctggc
    66421 tcacgatcaa acaatacagg agcaatcaga ttcagcttat ccaccagttc atttctgtcc
    66481 tgatgatggt agaacaccac acggtcccca taatccacac agactaactc gccattcctc
    66541 cgatcacgaa aacgcacact gccatctttc tgcagacggg cttcgagaac gccagtgtct
    66601 tcataaagtg gagttcctcc cggttcacag agaatcacgc agcggtcggc attcgttgtt
    66661 cgtcgcttgt ctttgcgacg atcacgataa tcccagccac gtaactgtga taatgcagct
    66721 ctgtctccct gtaccgcctg ttgctccacc cactgccggt aggacaaagg ataccactta
    66781 ccctccgcaa tcagtgacaa ccgctcttcc ttcacactct ccttcagcct gatcagcgcc
    66841 tgcatacgct gcacttctgc aatatggtaa tgcagcttac gcaattgtgg atcccggaac
    66901 tgcacacgga tatacgcctt gcgccgacgg caggcagcat gaatttcccg taaccgttct
    66961 ccgtaacgta agtcgggttt acgccagtgc tccttccatg caagataacg cgcgcgcaaa
    67021 tcttcccgcg cagctgcacg ggcttcacgg cgttcacgac gcagtacagg atcgcgccgg
    67081 aagccgggct ccacttcatc gctggcagca agttccggat tgtagcgaca ttcaggcttc
    67141 acccgttcaa atatatctgc acctgcaatc tcaaatggcc ctgcctgcgg ctcagccctg
    67201 gataacgtca aatccgggtg gatactgctg gccttcactg gtgtgagctc atgattaaac
    67261 gcatccacaa tcacaagacc atgatgctgt ttctgcaaca taagcccttt acgcgcaaac
    67321 atctcatggc acgcctgcca gctgaaattc ccgcccgcga tcatatcttc aatcaacgac
    67381 tcacactgaa cagtcgtgta cccccacaga ctttccttat cagaaaacag ttcgcgcttt
    67441 attgctccct gtggacctgt ccccatccgc atacgatacc attcgtaatc actgacagcc
    67501 tgagtaccaa gattacgtcc ccggaatcgt tctccgggta taacctgagt aaaaatatcc
    67561 ttcggtacag gctgccatcc accatccaga tcctgcatat aatccagcgt cattaacggc
    67621 cacacactgt ctgcacgtac tggtgtctgt tgtttatcaa atccatcatg cagtaccaga
    67681 tgcccgtgct ggatccccat ccataatcca gcctttgcca gagttctgtg cacatccagg
    67741 caactttcca gctcaccatt tctcgccatt tccggtaagc gttcggcaaa atgacggcag
    67801 gcgtactgtt tcagactctc cgtttctgcc actttttccg gccggatatc aacgtttatg
    67861 gcttccggat cataacgacc gggtgtcccc acctgactga aaatatctgt cggtacaggc
    67921 tgatattccc ccagtttccg ccccagtttt tcagctgtcc atgacggtcc gaacgaactg
    67981 agtgcaacac cttcccgcgc tctgtcccag ccatccttca ccaccagttc cccttcctgc
    68041 atcgtgatat acagaccatc actggccagc cgtttatgca aagacaacca gtcttgtgcg
    68101 ggctcttcac gcagcccggc aatcgacatc tgcgcaatat attccctgaa cgtctgcttt
    68161 ttcccgcgtc gccaggcatt acggcgctca cgtaccagcg ctgttttgcg cacaatacgg
    68221 ttgccgggtg catgcacaaa gcagccatta tcaggcgcga atccgtgctt aagttcaagt
    68281 tcacgacatg cacggctgag tttttcctgg ctccaggaaa gccagttaat ataaccagtt
    68341 tcaggatgta cacgattcac ggcaacatgc acatgcagat tatccgtgtc cgtatgaacg
    68401 gcagccacgt actgatgttt tgagagttca agagatttca gtgtgtgacg cacacagtca
    68461 aaaagctgct caggacgcgg gctttcatgc gcaggccagg aaagaatgta gtggaaaacg
    68521 gggtctgtat cattacggga aaaaacagct ttgtccgcct tgtactgcat ctcttcagca
    68581 gctgtttcca gtgaattgca gttatggaaa caggttacgc catagaaatt tacccactga
    68641 ctgccgtctt tcatcacatc aatcagggag acaaattttt cgttacgcag gcgtgttgca
    68701 taatcaacaa gccgtctgaa acggttgcgg tgagggacat cactcttgat ttcgctgtct
    68761 tctgtgagct cattttcctg cacatcatcc cgcacagacg tgtacgcaat caggtcttca
    68821 aaggaagacc tgccgtcacg tcttttgtgc ggaataatgg cattcatcag cagttatctc
    68881 agcgtcccat taagtctctt cgcagagcat tgacatattc agtaatggca acaagaacct
    68941 ctgcatactc cttatcacca gtgcgttttc cctccacaaa cagatgcttc tgaaggcggc
    69001 caagacgcat aatctcatca atcatacggt cgtcagtacg gcagttaacc accttatcca
    69061 gtgaagaact tcgtataaat gttgaaaccg tctgtccact tgactccgct ttttctctta
    69121 cgatttcagc ctcatcctcc gtgaatctcg ccgtgaattt tacaattctc ttcctgttct
    69181 cactgcccgt tcgggttttc ttctcactca tggtctgctc cttaccgggg tgtcggggca
    69241 aagccctgac caggtgggga atgtctgagt gcgcgtgcgc ggtccgacat tcccacatcc
    69301 tgtcccgttt ttcagtccat tatagcctgt tatttttgtg catcatagtc ttatgcagac
    69361 ggcagaggca gggcgcagcc ctgccaggag tcagtttttg atgataaaat caggcttcaa
    69421 cattaaacgg agagaaagtg ccaatggcgt ctaaaaaatt ttactcagat gatgacatcc
    69481 agctggcaaa agctgcttta tcggaattgc cagacctcac cgctcaacgg aaaacattgc
    69541 gtgattttct tgatgccatt cgtgatgaca tcattatcct ggtaaggaca aagggttata
    69601 cactgacaga tgttcgtgac acgctcaaaa atgcaggata tgaagtagga gagaaagcac
    69661 ttcgtgacat tattcgggaa gcagaatcga aaaaaccatc ccgccgttct tcctctaaaa
    69721 cagcacctaa aaagacagac tcaggcagga aagatggcat cgatatgaac aacaattgat
    69781 gtgacaatgt cacattgtgt tctgagatta tgatgattac agaaaaaaaa gccccggcat
    69841 aaatacctgg gcttttctgt ttaatcagac tcgtggcggc aatgcaccag gataatgccg
    69901 tgcaataata tcattcagtt tttcaacaag gtcagaacgc ttaaagacaa tgtgtacggt
    69961 ccctttctgg tagccacgca ccgtaaaata ttccccttct aacagcttac cgacattgtc
    70021 accgttcccg agaaattcac catactgcgc cccgatgctg ttcctgaagt caggaacgtt
    70081 gcgcccctcc agcagataaa acggacgtac caggtcatcc agtgcctccc gtccgctgca
    70141 gtccagcgtc acacgcccgt tactccagcg gtacagcagg ttttcaataa tgattttctt
    70201 acccagacgg cagggattat tggttctgta atcccatgac aatttccggt agacatcaat
    70261 cagcccctgt tcaaacgtct gcatcttgct ggcattaaga tggcggaatg ttgccagtag
    70321 gggagcccgc agaattcgga aaaaatcgta cgctaag
//
LOCUS       DAZOYF010000004        67027 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZOYF010000004 DAZOYF010000000
VERSION     DAZOYF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.004"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..618
                     /locus_tag="ACHUOF_000247"
                     /pseudo
     CDS             1..618
                     /locus_tag="ACHUOF_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            721..1044
                     /locus_tag="ACHUOF_000248"
     CDS             721..1044
                     /locus_tag="ACHUOF_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HFR0609594.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1111..2568)
                     /gene="nuoN"
                     /locus_tag="ACHUOF_000249"
     CDS             complement(1111..2568)
                     /gene="nuoN"
                     /locus_tag="ACHUOF_000249"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR0609595.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2575..4104)
                     /gene="nuoM"
                     /locus_tag="ACHUOF_000250"
     CDS             complement(2575..4104)
                     /gene="nuoM"
                     /locus_tag="ACHUOF_000250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR0609596.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4268..6109)
                     /gene="nuoL"
                     /locus_tag="ACHUOF_000251"
     CDS             complement(4268..6109)
                     /gene="nuoL"
                     /locus_tag="ACHUOF_000251"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR0609597.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6106..6408)
                     /gene="nuoK"
                     /locus_tag="ACHUOF_000252"
     CDS             complement(6106..6408)
                     /gene="nuoK"
                     /locus_tag="ACHUOF_000252"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR0609598.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6405..6959)
                     /gene="nuoJ"
                     /locus_tag="ACHUOF_000253"
     CDS             complement(6405..6959)
                     /gene="nuoJ"
                     /locus_tag="ACHUOF_000253"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR0609599.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6971..7513)
                     /gene="nuoI"
                     /locus_tag="ACHUOF_000254"
     CDS             complement(6971..7513)
                     /gene="nuoI"
                     /locus_tag="ACHUOF_000254"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR0609600.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7528..8505)
                     /gene="nuoH"
                     /locus_tag="ACHUOF_000255"
     CDS             complement(7528..8505)
                     /gene="nuoH"
                     /locus_tag="ACHUOF_000255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR0609601.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8502..11228)
                     /gene="nuoG"
                     /locus_tag="ACHUOF_000256"
     CDS             complement(8502..11228)
                     /gene="nuoG"
                     /locus_tag="ACHUOF_000256"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR0609602.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11281..12618)
                     /gene="nuoF"
                     /locus_tag="ACHUOF_000257"
     CDS             complement(11281..12618)
                     /gene="nuoF"
                     /locus_tag="ACHUOF_000257"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR0609603.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12615..13115)
                     /gene="nuoE"
                     /locus_tag="ACHUOF_000258"
     CDS             complement(12615..13115)
                     /gene="nuoE"
                     /locus_tag="ACHUOF_000258"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR0609604.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13118..14920)
                     /gene="nuoC"
                     /locus_tag="ACHUOF_000259"
     CDS             complement(13118..14920)
                     /gene="nuoC"
                     /locus_tag="ACHUOF_000259"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR0609605.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15014..15676)
                     /gene="nuoB"
                     /locus_tag="ACHUOF_000260"
     CDS             complement(15014..15676)
                     /gene="nuoB"
                     /locus_tag="ACHUOF_000260"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR0609606.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15692..16135)
                     /gene="nuoA"
                     /locus_tag="ACHUOF_000261"
     CDS             complement(15692..16135)
                     /gene="nuoA"
                     /locus_tag="ACHUOF_000261"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR0609607.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16766..17704)
                     /gene="lrhA"
                     /locus_tag="ACHUOF_000262"
     CDS             complement(16766..17704)
                     /gene="lrhA"
                     /locus_tag="ACHUOF_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR0609608.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18623..19840
                     /gene="alaA"
                     /locus_tag="ACHUOF_000263"
     CDS             18623..19840
                     /gene="alaA"
                     /locus_tag="ACHUOF_000263"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR0609609.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19924..20523
                     /gene="yfbR"
                     /locus_tag="ACHUOF_000264"
     CDS             19924..20523
                     /gene="yfbR"
                     /locus_tag="ACHUOF_000264"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR0609610.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20582..22414)
                     /locus_tag="ACHUOF_000265"
     CDS             complement(20582..22414)
                     /locus_tag="ACHUOF_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR0609611.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22501..23151)
                     /gene="hxpA"
                     /locus_tag="ACHUOF_000266"
     CDS             complement(22501..23151)
                     /gene="hxpA"
                     /locus_tag="ACHUOF_000266"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFR0609612.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23162..23656)
                     /locus_tag="ACHUOF_000267"
     CDS             complement(23162..23656)
                     /locus_tag="ACHUOF_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR0609613.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23739..24194)
                     /gene="yfbV"
                     /locus_tag="ACHUOF_000268"
     CDS             complement(23739..24194)
                     /gene="yfbV"
                     /locus_tag="ACHUOF_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR0609614.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24532..25734
                     /gene="ackA"
                     /locus_tag="ACHUOF_000269"
     CDS             24532..25734
                     /gene="ackA"
                     /locus_tag="ACHUOF_000269"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR0609615.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25809..27953
                     /gene="pta"
                     /locus_tag="ACHUOF_000270"
     CDS             25809..27953
                     /gene="pta"
                     /locus_tag="ACHUOF_000270"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR0609616.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28143..29663
                     /gene="yfcC"
                     /locus_tag="ACHUOF_000271"
     CDS             28143..29663
                     /gene="yfcC"
                     /locus_tag="ACHUOF_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR0609617.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29696..30238)
                     /gene="yfcD"
                     /locus_tag="ACHUOF_000272"
     CDS             complement(29696..30238)
                     /gene="yfcD"
                     /locus_tag="ACHUOF_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR0609618.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30296..30847)
                     /gene="yfcE"
                     /locus_tag="ACHUOF_000273"
     CDS             complement(30296..30847)
                     /gene="yfcE"
                     /locus_tag="ACHUOF_000273"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR0609619.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30903..31547)
                     /gene="yfcF"
                     /locus_tag="ACHUOF_000274"
     CDS             complement(30903..31547)
                     /gene="yfcF"
                     /locus_tag="ACHUOF_000274"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR0609620.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31683..32330
                     /gene="yfcG"
                     /locus_tag="ACHUOF_000275"
     CDS             31683..32330
                     /gene="yfcG"
                     /locus_tag="ACHUOF_000275"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR0609621.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32387..32749
                     /gene="folX"
                     /locus_tag="ACHUOF_000276"
     CDS             32387..32749
                     /gene="folX"
                     /locus_tag="ACHUOF_000276"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR0609622.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32770..33663
                     /locus_tag="ACHUOF_000277"
     CDS             32770..33663
                     /locus_tag="ACHUOF_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR0609623.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33711..34601)
                     /gene="rpnB"
                     /locus_tag="ACHUOF_000278"
     CDS             complement(33711..34601)
                     /gene="rpnB"
                     /locus_tag="ACHUOF_000278"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR0609624.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34798..35571)
                     /gene="hisP"
                     /locus_tag="ACHUOF_000279"
     CDS             complement(34798..35571)
                     /gene="hisP"
                     /locus_tag="ACHUOF_000279"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR0609625.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35579..36295)
                     /locus_tag="ACHUOF_000280"
     CDS             complement(35579..36295)
                     /locus_tag="ACHUOF_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0609626.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36292..36978)
                     /gene="hisQ"
                     /locus_tag="ACHUOF_000281"
     CDS             complement(36292..36978)
                     /gene="hisQ"
                     /locus_tag="ACHUOF_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR0609627.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37068..37850)
                     /gene="hisJ"
                     /locus_tag="ACHUOF_000282"
     CDS             complement(37068..37850)
                     /gene="hisJ"
                     /locus_tag="ACHUOF_000282"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR0609628.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38071..38853)
                     /gene="argT"
                     /locus_tag="ACHUOF_000283"
     CDS             complement(38071..38853)
                     /gene="argT"
                     /locus_tag="ACHUOF_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR0609629.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39119..39688)
                     /gene="ubiX"
                     /locus_tag="ACHUOF_000284"
     CDS             complement(39119..39688)
                     /gene="ubiX"
                     /locus_tag="ACHUOF_000284"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR0609630.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39783..41300)
                     /gene="purF"
                     /locus_tag="ACHUOF_000285"
     CDS             complement(39783..41300)
                     /gene="purF"
                     /locus_tag="ACHUOF_000285"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR0609631.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41337..41825)
                     /gene="cvpA"
                     /locus_tag="ACHUOF_000286"
     CDS             complement(41337..41825)
                     /gene="cvpA"
                     /locus_tag="ACHUOF_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR0609632.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42084..42746)
                     /gene="dedD"
                     /locus_tag="ACHUOF_000287"
     CDS             complement(42084..42746)
                     /gene="dedD"
                     /locus_tag="ACHUOF_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR0609633.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42736..44004)
                     /gene="folC"
                     /locus_tag="ACHUOF_000288"
     CDS             complement(42736..44004)
                     /gene="folC"
                     /locus_tag="ACHUOF_000288"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR0609634.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44074..44988)
                     /gene="accD"
                     /locus_tag="ACHUOF_000289"
     CDS             complement(44074..44988)
                     /gene="accD"
                     /locus_tag="ACHUOF_000289"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR0609635.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45144..45803)
                     /locus_tag="ACHUOF_000290"
     CDS             complement(45144..45803)
                     /locus_tag="ACHUOF_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0609636.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(45886..46698)
                     /gene="truA"
                     /locus_tag="ACHUOF_000291"
     CDS             complement(45886..46698)
                     /gene="truA"
                     /locus_tag="ACHUOF_000291"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR0609637.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46698..47711)
                     /locus_tag="ACHUOF_000292"
     CDS             complement(46698..47711)
                     /locus_tag="ACHUOF_000292"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0609638.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47777..48913)
                     /gene="pdxB"
                     /locus_tag="ACHUOF_000293"
     CDS             complement(47777..48913)
                     /gene="pdxB"
                     /locus_tag="ACHUOF_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR0609639.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49012..50007
                     /gene="flk"
                     /locus_tag="ACHUOF_000294"
     CDS             49012..50007
                     /gene="flk"
                     /locus_tag="ACHUOF_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR0609640.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50004..51182)
                     /locus_tag="ACHUOF_000295"
     CDS             complement(50004..51182)
                     /locus_tag="ACHUOF_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0609641.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51437..52678)
                     /gene="fabB"
                     /locus_tag="ACHUOF_000296"
     CDS             complement(51437..52678)
                     /gene="fabB"
                     /locus_tag="ACHUOF_000296"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR0609642.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            52837..54843
                     /gene="mnmC"
                     /locus_tag="ACHUOF_000297"
     CDS             52837..54843
                     /gene="mnmC"
                     /locus_tag="ACHUOF_000297"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR0609643.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(54964..55242)
                     /locus_tag="ACHUOF_000298"
     CDS             complement(54964..55242)
                     /locus_tag="ACHUOF_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR0609644.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55276..55824)
                     /gene="epmC"
                     /locus_tag="ACHUOF_000299"
     CDS             complement(55276..55824)
                     /gene="epmC"
                     /locus_tag="ACHUOF_000299"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR0609645.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55824..56633)
                     /locus_tag="ACHUOF_000300"
     CDS             complement(55824..56633)
                     /locus_tag="ACHUOF_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFR0609646.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56633..57457)
                     /gene="mepA"
                     /locus_tag="ACHUOF_000301"
     CDS             complement(56633..57457)
                     /gene="mepA"
                     /locus_tag="ACHUOF_000301"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR0609647.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57461..58546)
                     /gene="aroC"
                     /locus_tag="ACHUOF_000302"
     CDS             complement(57461..58546)
                     /gene="aroC"
                     /locus_tag="ACHUOF_000302"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR0609648.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58581..59513)
                     /gene="prmB"
                     /locus_tag="ACHUOF_000303"
     CDS             complement(58581..59513)
                     /gene="prmB"
                     /locus_tag="ACHUOF_000303"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0609649.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59679..60230
                     /gene="smrB"
                     /locus_tag="ACHUOF_000304"
     CDS             59679..60230
                     /gene="smrB"
                     /locus_tag="ACHUOF_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR0609650.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60334..61224)
                     /locus_tag="ACHUOF_000305"
     CDS             complement(60334..61224)
                     /locus_tag="ACHUOF_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR0609651.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61211..61735)
                     /locus_tag="ACHUOF_000306"
     CDS             complement(61211..61735)
                     /locus_tag="ACHUOF_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0609652.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61732..62202)
                     /locus_tag="ACHUOF_000307"
     CDS             complement(61732..62202)
                     /locus_tag="ACHUOF_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0609653.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62199..62747)
                     /locus_tag="ACHUOF_000308"
     CDS             complement(62199..62747)
                     /locus_tag="ACHUOF_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0609654.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(62752..63474)
                     /locus_tag="ACHUOF_000309"
                     /pseudo
     CDS             complement(62752..63474)
                     /locus_tag="ACHUOF_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63494..66134)
                     /gene="yfcU"
                     /locus_tag="ACHUOF_000310"
                     /pseudo
     CDS             complement(63494..66134)
                     /gene="yfcU"
                     /locus_tag="ACHUOF_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66216..66779)
                     /locus_tag="ACHUOF_000311"
     CDS             complement(66216..66779)
                     /locus_tag="ACHUOF_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0609655.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16702 a        18153 c        16687 g        15485 t
ORIGIN      
        1 atattatttc tgttttcact ccctctgatt atttttacca accctgggtc actgcattat
       61 cgccaacgcc ccgacacgta agtcctgagc aattagaaaa aacagttcgt tatcttacac
      121 aaactgtgca tccacgtaat gccggcaata ttgataatct gaataggtcc gcagagtata
      181 taaaagagtt ctttgtcagt agcggtgcca gagttaccgc gcagggcgtc cccgattggc
      241 tattacgact ctgcgcctgg aagccagaat tacccttacc cggcaatgtc ctggctttat
      301 cccgatcggg gagactttat tgctgtggtc ggcagaatgc aggatatcaa cgccgttcgt
      361 caggtaaaag cggcattatt gtcatcccgg gatttatctg tttattctat gaatgcccca
      421 gggtttattc ccggtattga tttttctgat cacctcaatt attggcaaca cgatattccc
      481 gccgtaatga ttactgacac cgctttttat cgtaataaac aataccactt gcccggtgat
      541 accgcagaca gattgaatta tcagaaaatg gctcaggtgg tagatggtgt tataaccttg
      601 ttatacaaca gtaaataata aaaatttctc gaggcgggtc cagaaacagc gacatgtaca
      661 ttttattttc cctcgtctgt tttaagatgc gaaacataaa attcaatgga ggaaaaataa
      721 atgggaatga ttggctattt tgcagaaatt gactcagaaa agattaacca actgcttgag
      781 tctactgaaa aacctttgat ggataatata catgacacgc tttcaggatt acgccgactg
      841 gaatcactgg atcgaagtga gctgcgtaaa cagttttcca ttaagcgact caatgaaatg
      901 gagatttatc cgggagtgac cttttcagaa gagttagagg ggcaactctt tgcctcgatc
      961 atgcttgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca agggaatcat
     1021 gcgctgacgg ttattgttgg gtgatggtgt cagagcgcca aaaaaaaccg ccgaacatgt
     1081 cggcggcttt ctgacttaca aactaacagt ttacatcagc ggcattgcta accgcacaat
     1141 actaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag aaatcagcac
     1201 cacgataccg cccgcgctgt actgccagtt tgacggtgca tcgcgacccg gttgttccgg
     1261 tgcgtgcagg tacaggctca ccgccacgcg caggtagtag tagaggccga ttgccgaacc
     1321 gacaaccacg gcacccacca gccaccacaa gtgtgcctgg acaccgaccg ccagcacgta
     1381 gaacttaccg ataaagccca gcgtcatcgg gataccggcc agagacagca tcatcaccgt
     1441 catcactgcc gcgaggatcg gacgatgcca gaacagaccg cggtaagaga acagggaatc
     1501 agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg cgccgaggct
     1561 gctgaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct cgccggtttg
     1621 cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt aaccgagcag
     1681 acgtttgatg ttggtctggc tcaacgccat caggttaccg aagatgatgg aggcaaaggc
     1741 gataatcgcc agtactacgc gaatcgcttc gctgtcaccc accggtgcgt agaggaacag
     1801 acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaggaaag tggaaaccgg
     1861 cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca gagagagttt
     1921 gaagccgagg ccaacaatca tcatgccgaa acctgccagc aacagcggct cgttgagcat
     1981 accgtcgccg aggtttttac ccagcgcgac aaacgacagg tcgccagact gcgcatacac
     2041 cagcgccata ccgaacagca ggaaagaaga cgctgcggca gaaaggatgg tgtatttgat
     2101 actggcttcc agcgaacgtt tctggcggaa agcgtaaccg accaggccaa acagcggcaa
     2161 agagatcagt tcaataccga ggaacagaga cgccagatgg tttgcattcg ccagcaggat
     2221 cccgcccagc gcggcaatta acaccaacag gtagaactca tccttgttgt cgttatagcc
     2281 ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata ccagcccggt
     2341 gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag cgcccgcctg
     2401 gccaacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga gcgtagcgtt
     2461 gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg tcaagccgac
     2521 gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg cgatttacgg
     2581 ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg cggagtgcga
     2641 ggtatccaga atcggctgcg gatagaagcc cagcagtacc agcagcacca ccagcaacag
     2701 gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa tctggctttt
     2761 cgctttaccg aagtaagcgc gatgcaacat cgccagcgaa taaacagatg caaagaccag
     2821 cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga acagaatcat
     2881 aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg caaagaacag
     2941 cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc gcatgtcgcg
     3001 ggtatggata cgttcataaa gctgaccgca gagaataaac agacccgccg ccgacaagcc
     3061 gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg tgtagattgc
     3121 aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt tgatatcggt
     3181 ctgagcgaag gtcatccacg caccgtagaa gatgccgata acacccagcc acatagcgat
     3241 tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca acaaaccgta
     3301 agcggcggtt ttcagcaaga tccccgcgag gtccacggaa ccggcggtcg gagcctggga
     3361 gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg cgaaggcgat
     3421 gaagaagccc agcatcaaca gatattccac accactggac attggcgtat tcagcagctc
     3481 ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca gcgccaggat
     3541 ggcgatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg ttgccgccgt
     3601 gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga agtacatcgg
     3661 caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa ggaacacgcc
     3721 gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat atttttcgat
     3781 ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca cgaccatcag
     3841 cagcgacaga ccgtcaatgg cgagatgaat agagatacca aaacgcggga tccacggcat
     3901 gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat aaccgccctg
     3961 caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg cgatccagcg
     4021 tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa taaagggaat
     4081 taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc ccgtcaggac
     4141 cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt agtgtcggat
     4201 gcgccgcgaa ggcagcttat ccgacctacg ttcttatatg cccaggggca atcccacaat
     4261 ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg atgctcatgg
     4321 atgccacata ccagcgcaga taaccgttct cgcttaacag cagaccttta cctgcaaagc
     4381 gggaaagaac cgccgggatg ttcatcattg agttcagcgg atcgcgtttc agcaaccagg
     4441 caatacccag gaacggcttg acgaacactt tgtcgtacag ccagtcaaag ccccaagcgt
     4501 tgtaccacca ggtactcagc aggcggcccg gcgcactgtt ggcgatggag gtcaccagag
     4561 aacgtttacc cagccacagc caggctgcca gcagaatgcc gaccaccgcg accacgccag
     4621 aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc ggaagcacgc
     4681 cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc agcacaatca
     4741 gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct tttccgtgga
     4801 agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg accagacctg
     4861 ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca tccttactga
     4921 agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc aggaagcaga
     4981 gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc tgttcgtgat
     5041 ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag aacgcgtgag
     5101 tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg aacatgtagc
     5161 caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt accagcgcgg
     5221 caaaaccggc cagcagcagc gtaaccgccc cgacaatacc caccagatgc agaacttccg
     5281 gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg gttaccatcg
     5341 ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtca gcaagccatg
     5401 tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc agcgtcgccc
     5461 acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc atatcgcgga
     5521 agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg aaaacgtcac
     5581 ccacacgggt cacgacgaac gctttcattg ctgctgcgcc attcttcgga tcggtgtaat
     5641 agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg tacatcagca
     5701 gcaggttatc ggcgagaacc agaactacca tgctggcgat gaacaggttg gtgtaggcga
     5761 agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg tgaataagga
     5821 aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc accaggttaa
     5881 aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg tatgcctgct
     5941 cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc aggcccacag
     6001 agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag aacgccagca
     6061 ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc cgcgcatctc
     6121 acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca gcagcgcaag
     6181 gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca cctgaccgtc
     6241 ggtctgaccc cagtagcttc cggcgaccac aaaggccagc gcggaggcgt taatcatgat
     6301 ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg ttaagccaag
     6361 aacgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc gtgctcctcc
     6421 gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc acgctcttca
     6481 cgaccgacgt ggaaggccac aaccagaccc gcgagcagca gcatagaagc cagttccacc
     6541 gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat tggcgtaccg
     6601 tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag catgatggcc
     6661 gacaaaattg ccgggccaat ccacacctgc ggtttcagcc actggcgttc ctgttcgatt
     6721 tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat aatggcaccc
     6781 gcgtagacga taatttccag cgcaccggcg aagtaagcgc ctagtgagaa gaacaccccg
     6841 gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt ggtatgggtg
     6901 atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc gaactccatt
     6961 gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg ttctctgctt
     7021 cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta tattccgggt
     7081 atttgcccgg accggagatc agcagatcct ctttctcgta aaccagatcc tggcgcttgt
     7141 attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag gcttcttcgc
     7201 acagaccaca gaaaatgcag cgtgagaagt taatgcggaa aaattccgga taccagcgac
     7261 cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct accgcacaga
     7321 ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg atacgaccac
     7381 gataacgggg cggcagatag accggctctt ccgggtacat tcgcgtttcg cgtttggcga
     7441 acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct actaacaatt
     7501 cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga cagccgccgt
     7561 taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg acattacccg
     7621 gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca taaagaacgc
     7681 ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc caccgaagaa
     7741 cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca cgaagaacag
     7801 accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct gctcggcttc
     7861 cggctggtca aacgggtgac ggtgacacac cgccacgccc gcgatggcaa aggtaataaa
     7921 accaaagaat tgcgggatga cgttccacac atgcgcctgg ctgttgacga tgtcggtcat
     7981 gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga acacttcgta
     8041 gctcagggtc tgcgcagaag cacgcatcgc acccagcagc gagtatttgt tgttacttga
     8101 ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga agaacaaaat
     8161 cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaattg caaaggccag
     8221 cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat ccgagaattt
     8281 cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct ggagcgaacc
     8341 gccccagcca acacggttag gtccataacg gttctggaac agacccagca ggcgacgttc
     8401 gccaaagctc atgaatgccc cacaggtgac aaccaccagc aggatcacca ccgctttgag
     8461 gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg tgcctccttg
     8521 agatcctcaa gatgcgcgcc agccagcacc ggagcaatgc cggacatacc catcggcaag
     8581 cccacctgcc ctgccgtcag tccttcggcg atttcaaccg gcagcgtgac cgtgttgcca
     8641 tcgtaactaa aggagacgcg tgtacctgcg ttcacaccca acttcgcggc atccgctggg
     8701 ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg ctgtgacaat
     8761 tcatcgctgc caaacaggtg gtagtacggc gcgatacgcc atttcccgtc ctgcggctgg
     8821 aagcgtgccg gtacgctggt gaagtaatcc agaccgtttt cgctggtttc aaacagacgc
     8881 acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt gttccacgcc
     8941 tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg cgcggtcggc
     9001 tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg ctgacgcggc
     9061 tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg cggttcacgg
     9121 gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt gatacccgcc
     9181 agttctggga ttttcgccac aacagcgtca attacatggt cgagctgcgt ccagtccact
     9241 tcacggctca gcaaggtgct gtgcagcgag tgcagccagc gccagctttc cagcatgaca
     9301 gttttgctgt cgtaataagc cggatcgtaa acctggaaga aacgttgggc gcgaccttca
     9361 ttattaatta ccgtaccgtc gctttcagca aagctggcag aggaaagcac cagatgggcg
     9421 ttttccatga tcgctgtgcg ttgatgatca accaccatca ccagcggcgc tttagccagc
     9481 gcagcattca cgcgggtagc agaagcgtaa cgatgcagat cgttttccaa caccaccacc
     9541 gcgtcggcgc gtccggtttc cagttcggtt aacgcttctt caagcgaacc gccacccata
     9601 atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac gtcagcgccg
     9661 cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat gctaccggcg
     9721 ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac gatgacgtcg
     9781 attttgtttt gcagctcagg ttcgataccg tcaaccgctg gtgcggagtt atccagcgca
     9841 tgggcaatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta agtccacgcc
     9901 gcgatatcat ccagacgagt gtcatcaacg ttggtaacaa acagcggatg cttcgcacgt
     9961 tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc tgcagccatt
    10021 tcgcgcgctt tacctttcac cgcctgacgc actgccagtg cgacgcgcgc gccggtctgg
    10081 gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg cagagccgga
    10141 gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc ctgctcaccg
    10201 tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc aaagttgctt
    10261 tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg cagaatatct
    10321 gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc acgacgctgt
    10381 actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg accacggtcg
    10441 cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg cagttcgccg
    10501 taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat gctcggcgca
    10561 aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt aaatacgccg
    10621 gtcgggcaaa tttcgaccag gttaccggaa aattcgcttt ccagcgtgcc gtcttccggg
    10681 cgaccgaagt agacgttgtc gtgcgcgcca taaacgccca gatctgtacc gtcagcgtaa
    10741 tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc gtgagagatg
    10801 aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg acggaagctg
    10861 tgtccggtca tcacggtcat atcctgaaga tggcagttac cgccttcttc acataccgga
    10921 cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa ctgtttcgct
    10981 tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca ggacatcacc
    11041 aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg gcggcaagca
    11101 ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc cagagacaga
    11161 caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc tacatgaatt
    11221 gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat cgaaattcgg
    11281 ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg tattgctgaa
    11341 cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc tctgtaacgg
    11401 ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga atcgacacag
    11461 ttgctcaagt gtttcgatat cgcccggctg gccttcgcca cgctccagcg cacgcagaat
    11521 tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg actcacgggc
    11581 gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt caaccgccat
    11641 cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt ccatcggcag
    11701 atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc aggctttaaa
    11761 tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg cggtggtgcc
    11821 gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca tcagcttggt
    11881 gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag cgaggatcgc
    11941 cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc atacgccgga
    12001 ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca gggagttgat
    12061 taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga acagttcgaa
    12121 gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg cttcggcaat
    12181 ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga tgtagccacg
    12241 gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt gcggcagttg
    12301 ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt tacacagcag
    12361 gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc cagtcgaaaa
    12421 gcccgcgccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga tttcgtccgg
    12481 agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc ggtattcgtc
    12541 cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg tttcgggagt
    12601 acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc cggggtcaga
    12661 tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt ccccaggcag
    12721 caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt gatgttcagc
    12781 tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac cacgctgtcg
    12841 caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta gaacgttgcc
    12901 acaccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg gatcgcacca
    12961 tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga cgccgcacgc
    13021 gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc actcagctca
    13081 aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca tctgacataa
    13141 caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg cggatcgccg
    13201 ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga taactcatgg
    13261 tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca atcatctgga
    13321 aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg gtgatcaggg
    13381 tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg tgatccgctt
    13441 tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga ctctggcgca
    13501 gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacca ccaaccggga
    13561 tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg tcgaagtcga
    13621 tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct ttcgcgccat
    13681 aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc gctttctcgt
    13741 aagacgccag acgtttcggc atccagtcga ggaactcacg cagcagacga tcccagccgc
    13801 gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata cggaaacccg
    13861 tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag aacacgggcg
    13921 tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg ttgatacgga
    13981 acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg atcccggcca
    14041 gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg tattcaatac
    14101 ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc tccgcaccac
    14161 ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg agttgtaaaa
    14221 cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg aacatgaagt
    14281 cctcattttc ggtgccgcgt ttcatccccc actcttccgg tttgaaggtc agggcttcca
    14341 tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta gcgcgtgccg
    14401 ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg cggcgcaggt
    14461 tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt ttcacgctca taccagttag
    14521 cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc agcgccacct
    14581 tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg gaaaaatccg
    14641 cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt aagtcaaaca
    14701 gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt aattgttcac
    14761 gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag gcatccggcc
    14821 caaaacggtt gcgcagttcg ccaattaccg gatcatcaag atgatcgcgg gtctgccagg
    14881 cgggttcttg cgcggttaag tcagtcatat tgttcaccat tgcaaatggt ccgtggtgac
    14941 tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa atccctttgc
    15001 cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca atgcgttcac
    15061 cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga tcgccaacca
    15121 cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc agtgcctgca
    15181 tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg atgaatttat
    15241 cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca caggcaccca
    15301 ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata accggtgcca
    15361 ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc gaagcacgca
    15421 atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc accatctcaa
    15481 cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga ccccagttaa
    15541 ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc agagggtcgg
    15601 ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga tctatgcggg
    15661 tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg atactgttcg
    15721 tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc gcgccaatac
    15781 gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct tccacaaagc
    15841 ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc gcttcaacgt
    15901 cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt aagcgggcgg
    15961 agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg cgtgcgcgac
    16021 cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca acgataagaa
    16081 agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata ctcattgctt
    16141 actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc cacatcgatt
    16201 catggggagg aataaaaaaa accttacaat aactgtagaa attcttttat acagctaatt
    16261 gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag cttcttcaca
    16321 ttagtttaca taatatcaac accattagca tttaatgccc tttcacccca gatccttgac
    16381 gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg gaaaaaatac
    16441 gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt attttcttga
    16501 tctgtgacac gcttttgtta ttccataaca aaaacgcagc aacaaattta cgtatttttt
    16561 aacatcattg tagcaggtga tttttttcag gcgattattt gtgcattcgg gacgtgaatc
    16621 tctggtgatt gaaaaataaa cagttttgta cgttctgcac tatgcgagga aggattttta
    16681 ctaaaaaaaa gccgctgggg tttaaaacac ccccagcggc tcgtttttta cactattgtc
    16741 tcaggaatta tctatcgtcc gtcgattact cgatatccct ttcaatcaac aatgaatcat
    16801 ccccttccgg agcagacatt gggctgtatt gccacggatt atggtagctc tccatcgcct
    16861 gatagatcac ctgggccagc tcattattac tggacgggtc atagcacagc aaatattcgg
    16921 tatcaggtag cggcggtaag ccatctactc cactcagcac acgcaggtcc gggctcatca
    16981 tctcaaccgg ccttgccgtc acgccaagac cggctttcac tgccgcacga acggccggaa
    17041 gcgtcgaggc gacataagcc agtcgccatg gaatatctac tttattaagc gtcgccagca
    17101 ccatatcgcg aaacggactt ggatcatcca gcaatacaag agggatcggc tcgccttttt
    17161 gcaatacgta ttccgctgcg cagtaccagt gtgttggcga ggtacgcagg ttcaatgcct
    17221 taaacgctga aggacgatgg gtggttacca tcaaatccac ttcctgggat tccagcattt
    17281 ctgccatata ggcattacgt ttaacgcgga catccagcgc taatttcgga taaaccgaac
    17341 tcacgcgatt taacaggaaa ggtaagatcg tatcggcaga ttcatctgaa gcaccgatag
    17401 ttaacacgcc ctgaagatta ctgaacatta atgatgagca ggcctcatca ttaaaacgca
    17461 ggattttcct ggcgtaacca agaagttgaa tgccatgttc agttaacagt ttgttgcgac
    17521 cgtgacgagc gaacagttct ttcccaacga gttgttccag acgctgcatt tgctgactta
    17581 cggcggactg agtacgacac acagcggcag ctgcggcagc aaaagtgttc agatcggcaa
    17641 cagcaacaaa tgttctcagc agatcgaggt cgaggttaat tatcggacga tttgcactta
    17701 tcatatatta tcacttactg gcggctcata ctgagctggt taatgctgtg cacacacaaa
    17761 caagcaattc catttgtaat gtgcctccct ggcagtttca tccggaaccc ggacgaaagt
    17821 aaaaatgcat atgagttgca ctaaaaaagc gactcacatt gttccgttat aatgcctgaa
    17881 gtagatcaca gaatatatct tcagggatcg catatctatt aagttactca ctcttttcta
    17941 tttatgacat gcgcgtgttt gtataaatgt aaatgtgagt ccttgttcca ctctcgtgca
    18001 gcatcgctgg tcatacgcga acacgtacaa acagcaatgg tgaagcggca tcaagagcag
    18061 ggatccgtca tttatgcgag cattttaccc caaaaacctt ttatttataa gggtcattgc
    18121 gaattatctg atgcaaagtt atgttatgtt aggcaaagta atcttctatt attaataagc
    18181 acatcaaaac ctttttgaat attaaataat aattaattag catcatcctc attcattaat
    18241 tccgcttaac aatagtttca caccttaccc ccattgcgac acctcggaaa tcatcacgct
    18301 gttaaactct ccgtaatgcg gaaatatcat catcgcgttt cattagtgaa ttcttaaatg
    18361 aggcattttc acacaattat cttacagata aaaaaaccag acttgcaatt aagaattaga
    18421 acaattcact atataacatt gcatgtaaag catataagcc taattatttg tcattattaa
    18481 gttattaaca gcacaatcga gccttcccct ctggcaaaat cttattctgc agaccttcaa
    18541 aacaccgtcc tgggggagta cattgttcta agctgacttc cacggcaggg agtggcgata
    18601 acagcaaaaa aggtcaagat tcatgtcccc cattgaaaaa tccagcaaat tagagaatgt
    18661 ctgttatgac atccgtggtc cggtgctgaa agaagcaaaa cgcctggaag aagaaggtaa
    18721 caaggtactg aaactgaaca tcggcaaccc agccccattc ggttttgacg cgccagatga
    18781 aatcctcgtt gacgtgatac gcaacctgcc taccgctcaa gggtattgcg attccaaagg
    18841 gctttactcc gcgcgtaaag ccatcatgca gcactaccag gctcgtggca tgcgtgatgt
    18901 taccgtggaa gatatttaca tcggcaatgg tgtatcggag cttatcgttc aggcaatgca
    18961 ggcattgctg aacagcgggg acgaaatgtt ggttcctgca ccagattacc cactctggac
    19021 cgcggcggtt tcgctttcca gcggtaaagc ggtgcattat ctttgcgatg aatcctctga
    19081 ctggttcccg gacctcgatg atattcgcgc taaaattacg cctcgtacgc gtgggattgt
    19141 tattatcaac ccaaataacc caaccggcgc ggtatattcc aaagagcttt taatggagat
    19201 tgtggagatt gcacgtcagc ataatctcat tatcttcgcc gatgaaattt atgacaaaat
    19261 tctctacgac gatgctgagc atcactcaat tgcgccgctg gcacctgacc tgctgaccat
    19321 tacctttaac ggactgtcga aaacgtaccg cgttgcaggc ttccgtcagg ggtggatggt
    19381 gttgaacggg ccgaaaaaac acgccaaagg ctacatcgaa ggtctggaaa tgctggcttc
    19441 aatgcgcctg tgtgctaacg ttcctgcgca acacgccatt cagaccgcgc tgggtggtta
    19501 tcagagcatc agtgaattta ttacccctgg cggtcgtctt tatgagcagc gtaaccgcgc
    19561 gtgggaactg atcaacgata ttccgggcgt ttcctgcgtg aaacctcgtg gtgcgctgta
    19621 tatgttcccg aaaatcgacg ccaaacgctt taacattcat gacgatcaga aaatggtgct
    19681 ggatttcctg ttgcaggaaa aagttctgtt ggtgcaaggg acggcattca actggccgtg
    19741 gccggatcac ttccgcattg tcacgctacc gcgtgtcgat gatatcgagc tgtctttgag
    19801 caagttcgcg cgtttccttt ctggttatca tcagctgtaa tcttaatttc actgccggag
    19861 attgcatccg gcagtgttat cccgccacaa tgacctgatg atgtcatcat acgtaaggtc
    19921 actatgaaac agagccattt ttttgcccat ctctcccgcc tgaaactcat taaccgctgg
    19981 ccgctaatgc gcaacgtgcg gacggaaaat gtgtccgaac acagtttgca ggtagcgatg
    20041 gtcgcccatg cgctggcagc tatcaaaaat cggaaatttg gcggtaatgt caacgccgaa
    20101 cgtatcgctt tactggcgat gtaccacgat gcctcagaag tgctcaccgg cgatctccct
    20161 actccggtga aatacttcaa tgcgcaaatc gctcaggagt acaaggctat tgaaaaaatc
    20221 gctcagcaga aactggtcga tatggttccg gaagaactgc gggatatctt tgcgccgtta
    20281 attgacgagc atgcatatag cgatgaagaa aaatcgctgg tgaaacaggc agatgcactg
    20341 tgtgcatatc tgaaatgtct ggaagaactc gcggccggaa ataatgaatt cttgctggca
    20401 aaaacgcgac tggaagcgac gcttgaagcg cgtcgcagcc aggagatgga ctacttcatg
    20461 gaagtatttg ttcccagctt ccatctttcg ctcgatgaga ttagccagga ttcaccgctg
    20521 taagcagccg gagtctgcgt cgcatcagtc aataagcgcc ggatgcgaca tcaggctctt
    20581 gtcaaaacgg aaacagcatc gggatcatca ccacacaaac cgccatcacg ataatggtga
    20641 acggtacccc caacttcaca aagtcactga agctgtaatt tcccggacct aaaaccaggg
    20701 tgttaacagg tgaagacacc ggtgtcataa aggcggcgga tgctgccatc gccacgacca
    20761 tcgcaaatgg ataaggcgac actcccatcg ttttggcagc agccagcgca atcggagcca
    20821 tcaacaccgc cgtcgcggta ttagagataa acagcccaat aaccgccgac aagacaaaca
    20881 aacaccccag catcatatgt ggcccataac cgccgccaat atccatcagc cctttcaccg
    20941 ccagcgcgac acctcccgtt ttctgtaatg ccacagcaaa cggcatcatc ccaacgatca
    21001 aaataatgct cggccagtga atggatttat aggcgctttc agcatctata cagcggaatt
    21061 tccccatcag caggcaggcg atgatagcgg caacaggatt aggaatttca tttgtcagca
    21121 ttaacgccac cattagcacc agacagaaaa tggcatgggg tgcctggctg tgcgcgggcg
    21181 atgcttcact cacctcttcc ggtaagttca gcgctacgaa gtcgcggccc tgtttggcca
    21241 gcataccgat cagtttccag ttacccacaa ccaggatgat atcgcccagc agcagaggct
    21301 catccgccag cgaaccttcc agcgccacgc cattgcgctt gagccccacc acattcagtc
    21361 cgtagcgggt acgaaaacca atttcgcgca ccgatttacc aatcagttct gactcaggaa
    21421 ttaatgaaat ctctgccatg cccacatcaa gggcctggtc agaaaaatac tcgccgcgca
    21481 gtaccatcgg ctccagcaat tgctcactac aaaattgccg gagatcgaca tcagccgcag
    21541 acatatcaat aagcaaaacg tcacgcgcgc gaaattcaga aaccccatta acgttcacga
    21601 taacgcgacg aaaacgccgc cagcgttcaa caccgatgac gttagcgcca taacgctcac
    21661 gtaatttgag atcatccagc cgttgaccta tcattggcga tccggggcga atagccagac
    21721 gtcgcgcacg cccggtcagt cgatattcac ggataagatc gcgaaaggtt cgacgcgtcc
    21781 agccttcgcg ctgcggggtc tgggtatccc ctttcagcat gaaacgcatc actaacatat
    21841 acaaaatacc cagcaccagc acgaccaggc caataggtgt tacgctaaag aaactgaagc
    21901 cgtgatagcc ttcacgcagc aattcactgt tgactaccag gttcggcggc gtcgccacca
    21961 gcgtcatcat gccgctaatc agcccggcaa aacttaacgg catcatcaga cgtgacggcg
    22021 aagtttgcat acgcatggca acgcttaaca ccacgggaat aaagatagcg acaacgctgg
    22081 ttgaactcat aaacgcgcca agccccgcga cggtcagcat caacaaaacc aacattttga
    22141 tttcactgtt gcccgccact ttgaccagcc atgttcccat tacggtggca acaccggtac
    22201 ggaccaaacc atcgccaata ataaacaagg cggcaatcag gacaacgtta ggatcagaaa
    22261 agccggaaaa tacttcgggg accgtcagcg ttccgcttaa tgcaaacgcg acaataacaa
    22321 acaaagcgac cgcatccata cgcactctgc ccgtcgcaaa caagacgatg gcaacggcca
    22381 gtaatgaaag aacccaaatc aattcaccgt tcacaactta tccttgttaa ttgaggggga
    22441 tgacttgatt ctgccataaa aaagccccga cgagacgggg ctaaatcatg atcaggtgtt
    22501 tcactgaata ataacatcgc catttggctg tttggtcaca gtaatttgct ccagactgtg
    22561 gaggaccaaa tcgacctcat tcaggcgcgg ggtatctgcc ggagcgttaa ccgcaatgac
    22621 atggcaaccc gccgccaggc cagaaagcac gccagcggga gcatcttcca ccaccacaca
    22681 ctcctgcggc gcaagcccca gcagctgcgc acctaacaga tacgcatcag gttctggttt
    22741 tccgcgcttc actcgctcag cggttacaaa cacctctggt gcgggaagcc ccgctatttt
    22801 atggcgcgct cgcgctaccg gcatggagcc agaagtcaca atggcccacg gaatacctgc
    22861 tttattcaaa tgattgagta aggcgattgc cccaggaagc gcggtaatac cttcggtttc
    22921 cgtggcctcg atttgctcca gacgcgtaaa ctcggcggca atatcagcct cggatttgcc
    22981 cgccataaaa tggcgcaaag aggtgatcgc ctgtttaccg tgaatgaaag ccagcacctc
    23041 ttccggcgct aacccatgac gtctggccca gttgctccac gcccgttcta ccgcaggcag
    23101 ggaatccacc agcgttccat caagatcaaa cagaaaacct ttgcaccgca cgcgggcctc
    23161 ctcaggcgtt gataatttga ttaatttcgt tggcgctcaa atggtactga cgcgggcagg
    23221 catgccacac attaagcatg cgctgatatt tttcccacat tggcgtctgg gcgttaaaac
    23281 catgagtccc agcgtcaaaa tgggtatagc gcccttccac attaaccata aagcggacat
    23341 aaccgaggta acgtgcttca gtggcggcgt caaagccgag gaaggtgaca cgacgttcat
    23401 cgatggattg ctggtcctgc aaattagacc aagaaacata caacgcatga tacatatcca
    23461 taatgtcgat gatagtgcgg caggtttctt ctttcagctc gccaaactcg cgatccaatt
    23521 cacgcatctg taatccgtaa ccacgctcaa taattgtttg caggcgacgg taacgttcag
    23581 catttgccgg atcgagcata gtcatcatct tgtactgatt agacaaaata agacgttgcg
    23641 cgttggtcat ttccattgtt gactcctgta tcactctact acggtgaaaa aaaagaaggc
    23701 tgagtatgcc ttcttttata tgcgtaatca ggggtcaatt acaaatcatc aaggaaagtt
    23761 ttatccagtt gtttgaaggc gcgcttaagc gtgtcagcta atgcctggta atcaggcttg
    23821 ccttcaacgg gtgccaacac ctgtccagac tcctgcaatt taccgcgaac ttcataaaac
    23881 cagttaagga ttgcaggggg taatggcgtt acagaacgct tgcccagcca ccacaatccc
    23941 tgcatgggta aacttaaggc gaacagtgca gtggcaactg ccggcccaag cagaccgccc
    24001 agggcaatct gccagcagag agtaaatacg gcgatcggcg gcataaaacg gatcgcataa
    24061 cgcgtcatct tgataacgcg attttcgaca aagaccggag caaggcgttt ttccagtggc
    24121 cacgtctttg agtaatgctg tccgcggcga aacaagctaa aaaaattaac agaacgatta
    24181 tccggcgttg acatgcttca cctcaacttc acatataaag attcaaaaat ttgtgcaaat
    24241 tcacaactca gcgggacaac gttcaaaaca ttttgtcttc catacccact atcaggtatc
    24301 ctttagcagc ctgaaggcct aagtagtaca tattcattga gtcgtcaaat tcatatacat
    24361 tatgccattg gctgaaaatt acgcaaaatg gcatagactc aagatatttc ttccatcatg
    24421 caaaaaaaat ttgcagtgca tgatgttaat cataaatgtc ggtgtcatca tgcgctacgc
    24481 tctatggctc cctgacgttt ttttagccac gtatcaatta taggtacttc catgtcgagt
    24541 aagttagtac tggttctgaa ctgcggtagt tcttcactga aatttgccat catcgatgca
    24601 gtaaatggtg aagagtacct ttctggttta gccgaatgtt tccacctgcc cgaagcacgt
    24661 atcaaatgga aaatggacgg caataaacag gaagcggctt taggtgcagg cgccgctcac
    24721 agcgaagcgc tcaactttat cgttaatact attctggcac aaaaaccaga actgtctgcg
    24781 cagctgactg ctatcggtca ccgtatcgta cacggcggcg aaaagtatac cagctccgta
    24841 gtgatcgatg agtctgttat tcagggtatc aaagatgcag cttcttttgc accgctgcac
    24901 aacccggctc acctgatcgg tatcgaagaa gctctgaaat ctttcccaca gctgaaagac
    24961 aaaaacgttg ctgtatttga caccgcgttc caccagacta tgccggaaga gtcttacctc
    25021 tacgccctgc cgtacaacct gtacaaagag cacggcatcc gtcgttacgg cgcgcacggc
    25081 accagccact tttatgtaac ccaggaagcg gcaaaaatgc tgaacaaacc ggtagaagaa
    25141 ctgaacatca tcacctgcca cctgggcaac ggtggttccg tttctgctat ccgcaacggt
    25201 aaatgcgttg acacctctat gggtctgacc ccgctggaag gtctggtcat gggtacccgt
    25261 tctggtgata tcgatccggc gatcatcttc cacctgcacg acaccctggg catgagcgtt
    25321 gacgcaatca acaaactgct gaccaaagag tctggcctgc tgggtctgac cgaagtgacc
    25381 agcgactgcc gctatgttga agacaactac gcgacgaaag aagacgcgaa gcgcgcaatg
    25441 gacgtttact gccaccgcct ggcgaaatac atcggtgcct acactgcgct gatggatggt
    25501 cgtctggacg ctgttgcatt cactggtggt atcggtgaaa atgccgcgat ggttcgtgaa
    25561 ctgtctctgg gcaaactggg cgtgctgggc tttgaagttg atcatgaacg caacctggct
    25621 gcacgtttcg gcaaatctgg tttcatcaac aaagaaggta cccgtcctgc ggtggttatc
    25681 ccaaccaacg aagaactggt tatcgcgcaa gacgcgagcc gcctgactgc ctgatttcac
    25741 accgccagct cagctggcgg tgctgttttg taacccgcca aatcggcggt aacgaaagag
    25801 gataaaccgt gtcccgtatt attatgctga tccctaccgg aaccagcgtc ggtctgacca
    25861 gcgtcagcct tggcgtgatc cgtgcaatgg aacgcaaagg cgttcgtctg agcgttttca
    25921 aacctatcgc tcagccgcgt accggtggcg atgcgcccga tcagactacg actatcgtgc
    25981 gtgcgaactc ttccaccacg acggccgctg aaccgctgaa aatgagctac gttgaaggtc
    26041 tgctttccag caatcagaaa gatgtgctga tggaagagat cgtcgcaaac taccacgcta
    26101 acaccaaaga cgctgaagtc gttctggttg aaggtctggt cccgacacgt aagcaccagt
    26161 ttgcccagtc tctgaactac gaaatcgcta aaacgctgaa tgcggaaatc gtcttcgtta
    26221 tgtctcaggg cactgacacc ccggaacagc tgaaagagcg tatcgaactg acccgcaaca
    26281 gcttcggtgg tgccaaaaac accaacatca ccggcgttat cgttaacaaa ctgaacgcac
    26341 cggttgatga acagggtcgt actcgcccgg atctgtccga gattttcgac gactcttcca
    26401 aagctaaagt aaacaatgtt gatccggcga agctgcaaga atctagcccg ctgccggttc
    26461 tcggcgctgt gccgtggagc tttgacctga tcgcgactcg tgcgatcgat atggctcgcc
    26521 acctgaatgc gaccatcatc aacgaaggcg acatcaatac tcgccgcgtt aaatccgtca
    26581 ctttctgcgc acgcagcatt ccgcacatgc tggagcactt ccgtgccggt tctctgctgg
    26641 tgacttccgc agaccgtcct gacgtgctgg tggccgcttg cctggcagcc atgaacggcg
    26701 tagaaatcgg tgccctgctg ctgactggcg gctacgaaat ggacgcgcgc atttctaaac
    26761 tgtgcgaacg tgctttcgct accggcctgc cggtatttat ggtgaacacc aacacctggc
    26821 agacctctct gagcctgcag agcttcaacc tggaagttcc ggttgacgat cacgaacgta
    26881 tcgagaaagt tcaggaatac gttgctaact acatcaacgc tgactggatc gaatctctga
    26941 ctgccacttc tgagcgcagc cgtcgtctgt ctccgccagc gttccgttat cagctgactg
    27001 aactggcgcg caaagcgggc aaacgtatcg ttctgccgga aggtgacgaa ccgcgtaccg
    27061 ttaaagcagc cgctatctgt gctgaacgtg gtatcgcaac ttgcgtactg ctgggtaatc
    27121 cggcagagat caaccgtgtt gcagcgtctc agggtgtaga actaggtgca ggcattgaaa
    27181 tcgttgatcc agaagtggtt cgcgaaagct atgttggtcg tctggtcgag ctgcgtaaga
    27241 acaaaggcat gaccgaaacc gttgcccgcg aacagctgga agacaacgtg gtgctcggta
    27301 cgctgatgct ggaacaggat gaagttgatg gtctggtttc cggtgctgtt cacactaccg
    27361 caaacaccat ccgtccgccg ctgcagctga tcaaaactgc accgggcagc tccctggtat
    27421 cttccgtgtt cttcatgctg ctgccggaac aggtttacgt ttacggtgac tgtgcgatca
    27481 acccggatcc gaccgctgaa cagctggcag aaatcgcgat tcagtccgct gattccgctg
    27541 cggccttcgg tatcgaaccg cgcgttgcta tgctctccta ctccaccggt acttctggtg
    27601 caggtagcga cgtagaaaaa gttcgcgaag caactcgtct ggcgcaggaa aaacgtcctg
    27661 acctgatgat cgacggtccg ctgcagtacg acgctgcggt aatggctgac gttgcgaaat
    27721 ccaaagcgcc gaactctccg gttgcaggtc gcgctaccgt gttcatcttc ccggatctga
    27781 acaccggtaa caccacctac aaagcggtac agcgttctgc cgacctgatc tccatcgggc
    27841 cgatgctgca gggtatgcgc aaaccggtta acgacctgtc ccgtggcgca ctggttgacg
    27901 atatcgtcta caccatcgcg ctgactgcga ttcagtctgc acagcagcag taatctcgtc
    27961 atcatccaca gctttgcgct gcggatatct gaaccggaaa taatcactat ttccggtttt
    28021 ttattctctt aatttgcatt aatcctttct gattatcttg cttaactgcg ctgcatcaat
    28081 gaattgcgcc atctcacttt gcatacttac cactttgttt tgtgcaaggg aatatttgcg
    28141 ctatgtccgc aatcactgaa tccaaaccaa caagaagatg ggcaatgccc gatacgttgg
    28201 tgattatctt ttttgttgct attttaacca gtcttgccac ctgggtagtt ccggtgggga
    28261 tgtttgacag tcaggaagtg cagtatcagg ttgatggtca aacaaaaaca cgcaaagtcg
    28321 tagatccaca ctcatttcgc attctgacta acgaagcagg cgaacctgag tatcaccgcg
    28381 tacagctgtt cacgacgggc gatgaacgcc cgggcctgat gaacttcccg tttgaagggt
    28441 taacctcagg atcgaaatac gggacagccg ttggcatcat catgtttatg ctggtgattg
    28501 gcggcgcgtt tggcattgtg atgcgtacag gaaccattga taacggtatc ctggcgctta
    28561 ttcgccatac ccgcgggaat gaaattctct ttattcctgc gctgtttatt ctgttttcac
    28621 ttggcggcgc ggtatttggt atgggagaag aggccgtcgc ctttgccatt atcatcgcac
    28681 cgctaatggt ccggctgggc tatgacagta ttaccaccgt cctggtgact tatattgcca
    28741 cgcaaatcgg ttttgccagt tcgtggatga acccgttttg tgtggttgtt gctcagggga
    28801 ttgccggcgt tccggtgctt tctggctccg ggttgcgcat cgtggtgtgg gttatcgcca
    28861 ctctgattgg cctgatcttt accatggtgt acgcctcacg agtgaaaaag aatcctcttc
    28921 tgtcacgcgt gcatgagtcc gaccgcttct ttcgtgaaaa gcaggcggat gttgaacaac
    28981 gtccgtttac ctttggtgac tggctggtat tgattgtcct gacagccgta atggtctggg
    29041 tgatttgggg cgtgatcgtt aatgcctggt ttattccaga aattgccagc cagttcttca
    29101 ccatgggtct ggtgattggc atcatcggcg ttgttttccg ccttaacggc atgacggtta
    29161 ataccatggc ttcatccttt accgaagggg cgcgaatgat gatcgcccct gccctgctgg
    29221 tgggtttcgc caaagggatt ttgctgctgg ttggtaatgg tgaagcaggt aatgccagcg
    29281 tgttaaatac tatcctcaac agcattgcca atgccattag cggtctggac aacgcggtcg
    29341 cggcctggtt tatgttgctc ttccaggctg tatttaattt cttcgtgacg tccggttctg
    29401 gtcaggcggc attaactatg ccgttactgg caccgcttgg cgatctggtc ggtgttaacc
    29461 gtcaggttac cgtgctggcg ttccagtttg gtgatggctt cagccacatc atttacccaa
    29521 cctcagcttc gttaatggca acgctcggtg tgtgccgggt ggacttccgt aactggctga
    29581 aggtgggcgc gaccctgctt ggactgctgt ttattatgtc cagcgtcgta gtgatcggcg
    29641 ctcagttgat gggctaccac taaaaatgtt aagagccgca attgcggctc ttttttcatt
    29701 ctgccgtttc agtctctacg gcttcatttt tggcattgcg cttcatccac aacgccagcg
    29761 ctttcagcga gtctggagtg aactcatcgc agcgtgcggt gatttcttcc ggcgtcagcc
    29821 agcaaactcc actgacttca tcttcctgta gggcgaaggg accgtgagag acgcaactga
    29881 acaacgcgcc ccagacacgg caatttttat cttcgaaata gaactgcccg tgctcggcaa
    29941 aggggacacc ggcaatgccc aactcttctt ccgcttcgcg acgcgcggat tccagcagtt
    30001 gctcatcggc ctgaactaca ccgcctgcgg tcgcatctaa catgccgggt aaaaagtctt
    30061 ttgtctcggt acgacgctgg accagaattt tgcccatgcc atcatgcacg acgatgtaag
    30121 ttgcacgatg acgcagacac tgtgcccgca tttgttcccg gctggcttgt gcaatgactt
    30181 cgttctcttc attgacaata tccacccatt cagtacttgc caaacgacgc tgttccatta
    30241 tcaggaaacc ttctttttct ggcgctctta cggcgcattt tgagttgtgg gtaaattacg
    30301 gattaatcgc gacctgcgca atgatacttt gatcattgag tgcgattaca ctaagtacgt
    30361 cattatccag catgccataa cccgccggat taccgccttt cggaatactc accgagccgg
    30421 ggttgaagtg gaaaatctcc ccccgctgtt ctgccactgg cagatgggta tgaccgtaca
    30481 ccagcacatc gttctggttt aaagcaggta gattttccgg gccaaaaaga tgaccatgcg
    30541 tcaaaaacag acgttgtttt tccagtaata cctgttgcca cggcgcggtt atcgggaaat
    30601 gcagcatcat ttgatccact tcgctgtcgc agttgccacg cacagcgata accttatgtg
    30661 ccacttcatt aagccgttca gcgactttgg ctggcgcgta accctccggt aaagcattac
    30721 gcgggccgtg attcaacacg tcgccaagga tcaccagcca ctgggcaccg ctttggacaa
    30781 acaactccac aacacgttcc gtcgccggta acgacccatg aatgtccgat gcaaacatca
    30841 gtttcatcac tcactcctcg tcgaaaaaac gtaagctatg atactggatt ctgtcgttgc
    30901 tatcagccag attgcttcgc cgaaagtgca ataaaacgct ggacagacgc tcgctgccac
    30961 tggaatgtcg cataatccac caggcgttct ggcacctcat cgccatgtag taccaggcgg
    31021 ttaatcatta gcgccagatc agtatcagca atgcaccatt caccaaataa attcggctga
    31081 cccagtgcta acaaatgttc tgccatcgcg aacagtttct ctgcactggc ttttccctcg
    31141 gccgttagtg gcgctttttt cgcccccgca aagacaacat ccgtcggacg ctcttcgcgg
    31201 atgggcatca gatcgctgcg cagccaggcc tgaatctgtc gtgcacgcgc acgattttct
    31261 aaatcaagcg gataaatacg ttcccaggtc ggtggcgcaa atcgatcttc cagatactcc
    31321 gcaatggcag aagattcact caactcaaaa tcatcgattt gtaataacgg cacacggcgt
    31381 gtctgaccgt aaccttgcca cgtcggctgc aaatgttcac cgctgtcgag gtcgatggtc
    31441 ttgatatgaa acgacaggcc tttttcctgc aacgccaccc aggcggataa cacataaggg
    31501 gagaaaaagt gggcatctga ccaaagcgtg atagcgggtt tactcattat gtcctcggca
    31561 ataaaggggg ctggctatca aactatagag cctttttgtc actgtcacgt gacgaatact
    31621 cacgtaagtc gccgcgcttt ctatacttgt ggtgttcaga tgattaaaca accggagctg
    31681 caatgatcga tctctatttc gccccgacac ccaatggtca caaaattacg ctgtttctcg
    31741 aagaagcaga gcttgattat cgcttgatta aggtagactt ggggaaaggc ggtcagtttc
    31801 gtccggaatt tttgcgcatt tcgcccaaca acaaaattcc ggcaattgtt gatcattctc
    31861 ctgccgatgg cggcgaaccg ttaagcctct ttgaggctgc tgccattttg ttgtatctgg
    31921 ctgagaaaac aggactcttt ttgagtcatg aaacgcgtga gcgcgccgcc acattacagt
    31981 ggttattctg gcaggtaggc ggactggggc cgatgcttgg gcaaaatcat cattttaatc
    32041 acgcagcccc ccaaaccatt ccttacgcta ttgaacgtta tcaggttgaa actcagcgtc
    32101 tttaccatgt actaaacaag cggctggaaa actcgccctg gctgggaggc gagaactaca
    32161 gcattgcgga tattgcctgc tggccgtggg ttaatgcctg gactcgcctg cgaattgacc
    32221 tcgcaatgta tccggcagtc aagaactggc atgagcggat ccgttcgcgc cctgccaccg
    32281 ggcaggcact gctaaaagca caactcggtg atgagcgttc ggatagttaa cagaaacagg
    32341 ttctcgtgta ttatttcatc ctaagtaaaa caacggagaa cctgcaatgg cacaacctgc
    32401 cgctattatt cgtataaaga accttcgttt gcgtacgttt atcggaatta aggaagaaga
    32461 aattaacaac cgtcaggata ttgttatcaa tgtgacgatc cactaccccg ccgataaagc
    32521 gcgcactagc gaagatatca acgatgcgct gaattatcgc accgtaacga aaaacattat
    32581 tcagcatgta gagaataacc gtttctcttt gctggaaaaa ttaactcagg atgtgctcga
    32641 tatcgcacgt gaacatcact gggtgacgta tgctgaagtg gagatcgata aactgcacgc
    32701 gctgcgctac gccgattcgg tatccatgac cttaagctgg cagcgttaat cgccatatcg
    32761 ggaggctgca tgaacatagt gatcaccgga gggacgggat taattggtcg ccatttgatt
    32821 ccgcgtttgc tggagctggg ccatcaaatt acggtagtga cgcgtaaccc gcagaaagcc
    32881 agttccgttc tcggccctcg ggtgacacta tggcaagggc ttgccgatca aagcaacctc
    32941 aacggcgttg atgcggtaat caacctggcc ggagaaccga ttgctgataa acgctggact
    33001 cacaagcaaa aagagcgtct ctgccaaagc cgctggaata tcacgcaaaa actggtcgat
    33061 ttgattaatg ctagcgacac gccgccgtcg gtactcattt ccggctcggc aacgggctat
    33121 tatggcgact taggtgaagt ggtggttacc gaagaggaac cgccgcataa cgaatttact
    33181 cataaactct gcgcccgctg ggaagagatt gcctgccggg cgcaaagtga caaaacgcga
    33241 gtgtgcctgc tgcgaaccgg cgtggtactg gcaccggacg gcggtattct cggtaaaatg
    33301 ctgccaccgt ttcgtcttgg cctgggcggg ccgattggtt ccggtcggca gtatctggcc
    33361 tggattcata ttgatgatat ggtcaacggc attctctggc tgctggataa cgagctgcgc
    33421 gggccattta atatggtttc gccctacccg gtacgcaatg aacaatttgc ccatgcgctc
    33481 ggtcatgcgc tgcatcgccc ggctattttg cgcgtcccca caaccgccat tcggctgtta
    33541 atgggcgaat cttcagtact ggtattaggt ggacaacgcg cgctgcctaa acggctggaa
    33601 gaagcgggtt ttgcgtttcg ctggtacgat ttagaagagg cgctggcgga tgtcgttcgc
    33661 tgatgtggtt tacagcaaac atccgccagt taactccctg tgttacagga ttagtggctt
    33721 tgcgcgataa gatcgtctgg tgaaagtcgg gtcaccatca taactaactc tctgtctaaa
    33781 cctctatcca gcatctcctg agcaatacgc agggcttctt cgtgtttgcc ctgcattgcg
    33841 ccttcttcac gtaatctgtc agcaatggtc atcagtttct ccttttcttg tggtgcgcgt
    33901 tctgttatct caccaataaa tgcacggaaa cgctgggcat cccctgtttg taatacgtaa
    33961 ttaaacaggg cttttagctg tctgtcatta gtgttccctg taactaacag cgaaacaatt
    34021 tggtcgacta atcccaacag atcgcgctga cgaatatgtt tctgaattaa ctccaacagc
    34081 gccattttgc ggtgttgcat aatctcgtca tccggcacca cggtaatatc caccaacgga
    34141 aacgctgatg aatatatttt gcgggctata gcaggctcgg caaattcatc aagccagcag
    34201 agtgaataag gataaggact tctgcaacca tgataaaaca gcatcgggat caccaatgga
    34261 agctctttat agcccgcatc aagatggttt tgcattgccg caatggaata acgcatcatg
    34321 cgaaaagcca ttaattcttc cggcttactt tggtgctcta tcactacata aatataaccc
    34381 actccctcct gcgttttcac agaccacaag aggtcggaat aatattgccg caggtcttca
    34441 tcaataaaac tgtttggttc tagtttaagc gtcgttaaat cacacagttt gcgcagcggc
    34501 gcgggaagat gaatattaat aaaattccgc gcggtgtctg gatggcgtaa aaaagattta
    34561 aataccgcat catgcggcgt ggaagttgtc gatattgtca tggcgttccg tcacccctca
    34621 aatttaagat gacgcgacaa taaccacgat taacacccaa tgcagcaggc agtttttatc
    34681 tttggacagc gaattcagaa gtatttttag cggactgcaa cttttacatt tcagcggaag
    34741 ctgactcgca aattcccctt gaaaatactc cggcagcgtt agccaccgga gaggaaatta
    34801 tttcagcgat cccttaagga actgttgcag acgagggctt tgcgggttgc caaataactg
    34861 ctccggcgcg ccctcttctt ctattttccc ctgatggagg aaaatgacgt gagtagaaac
    34921 atggcgagca aagcccattt cgtgagtcac taccaccatg gttttcccct cttctgccag
    34981 ttgctgcata atacgtaaca cttcgcctac cagttcagga tcgagcgccg aggtaggttc
    35041 atcaaacagt aaaacttctg gttccatcgc cagcgcccgc gcgatagaaa cacgctgttg
    35101 ctgaccgccg gaaagatgca ccggatattt cccctgcgca cgttcgtcta tcccgacttt
    35161 cgccagatac ttcaccgccc gctcgcgcgc ttcctgcttg ctcaggccca acacctgaat
    35221 cggcgcttcc atgacgtttt ccaacaccgt catatggctc cagagattga agtgctggaa
    35281 taccatcgtc aggcgtgtgc gcagtaagcg cagttgattt ttatcggcga ctttgagttg
    35341 accgtctttg tcgcgcacca gattgatcgt ctggccattg accacgatcg atccttcact
    35401 cggtttttcg aggaagttaa tgcagcgcag aaaggtactt ttccccgatc ccgacgatcc
    35461 gatgatgctt attacatctc cggcattcgc ttgcagtgag acccctttca gcacttcgtg
    35521 ttcgccatag cgtttgtgca aatcgataac gtttaattta ttctcggaca tcgtattctc
    35581 agtgcgttga agaaggtttc acatgctgca accagcgttt ttccgctctg cgaaagaggc
    35641 tgatcaggac ataagagatg attaaataga gcaccgcggc aatgccgaag gcggtaaaag
    35701 gttgatacgt ggcggcgtta atatcgcggg ctattttcag cagatccggc acagtggccg
    35761 taaacgccag cgcagtagag tgcagcatca ggatcacttc gttgctgtat gccggtaacg
    35821 caatacgcag cgcagaaggc aaaataatgc agcgatacat tttaaaggtc gagaagccat
    35881 aggctctggc ggcttcaatt tccccatgcg gaaccgaacg gattgcccca gcaaaaatct
    35941 cggtagtgta agcgcaggtg ttaagcgtca gcgccagcac ggtacagttc aggccactgc
    36001 ggaagaaagc gttaaggaat tcggttccct taacaatctc aagcgtgtac atgccggaat
    36061 agaacaccag caactgaaca tacagcggcg taccgcgaaa aatataggta aataaccaga
    36121 ttggaaactg gatgtattta ttactggaga cacgaccaat cgccagaaac agcgccagga
    36181 ctccgcctat cactaccgac aaaataagca gccacagagt gatcgccaca ccagtaaagc
    36241 gataaccgtc ggtccacagc agcggtttcc agtattcatg taagatttcg atcacaggtc
    36301 agccctcttc acacccacgg agtagcggcg ctcaaggaac agcagcacac cattggaaac
    36361 ggtggtgaaa accaggtaaa tcacgccaca gacgatggcg aaatagaacg gttcccaggt
    36421 acttttgcct gccagctgcg tggctttgac cacatcttcc aggccgagta acgaaaccag
    36481 tgcggtagat ttgaggatca cctgccagtt gttgccaatg cctggcaggg cgtaacgcat
    36541 catcgccgga aacatgatcc gccgaaacac ttgcccgcga gtaaaaccga acgccgtcgc
    36601 cgcctctata tgccctttcg gcactgccat aaacgcgcca cggaacgttt cggtaaaata
    36661 agcaccgtag ataaaaccga gagtgataat accagcgacc atcggatcga tatcaatctg
    36721 cccgacgccc atcgcctccg tcaccatgtt tagcgcaatc tgcaaaccat agaaaatcag
    36781 cagcatcaac accaagtccg gcacgcctcg aatcagcgtg gtgtaccctt cgaaaatcag
    36841 ccccgaaagc cgattttgcg agagcttacc gccagcgcca attaaaccga tgattacagc
    36901 gagcactaca gagctgatag ccagctccag cgtgacgagc gcaccctgta aaataacacc
    36961 tgaaaaccca tacaacatgc tgcctgtcct gtcgtgtgtg gtgaattacc gcctcttgtc
    37021 tccctccggt tacccgaagg gagcgggctt tctttcaccg atggcgatta accaccataa
    37081 acatcaaaat cgaagtactt tttcgctaat ttctcgtaag taccgtcagc gcgcatttcg
    37141 gcaaaggctt tgttcagtgc ctcgcgcagt tcgttatctt ctttacgcag gcccatgccg
    37201 gtccctacgc caaacagttt ttcatcttta acagacggac caccgaattt gtaatcttta
    37261 ccgacgggtt gtttgaggaa accttcgctg gcagcgacct catcctggaa cgcggcatca
    37321 atacgtccgg cagtcaggtc agaataaatg ttgtcctgcc cctgatacga gacgatttca
    37381 atgccttttg gtgcccagtg ttcattaccg aacgtctcct gggtggtgcc ctgcaatacg
    37441 cctacccgtt tgcctttcag cgactcgact gtcggctgaa tgtcagaatt tttcgccacc
    37501 accagacgag aatcggcagc gtacagtttg tcggtgaagg caatttcttg ctgacgtttt
    37561 tccgtaatgg aaagcgatga catgatggcg tcaatcttct tcgcttttaa ggacgggatt
    37621 aacgcatcca gcgggttttc gacaaacgta cattgcgtat tgatgcgctt gcacaactcc
    37681 ttcgccagct cgatatcgaa accaaccagt tcgccttgtg aattttttga ttcaaatggc
    37741 gcataggtcg ggtcggtacc gatgcggatg ttttgcggaa ttgcagcaag tgccgcagtt
    37801 gcgctggaga aggccagaac cagagagagc gatagcacca gttttttcat aatgtcctca
    37861 actgacagtc tttttatagg gaatatttac aggtttgtta cacttatcgt gccataaaat
    37921 tgaccatcaa ggcaaaatat cccgccccgt tagacgtatt atttttcatt aaaaatgctt
    37981 aagtatgcat ttcaatgcac cacgatagtg caacaaacct ggcgtgcacc atgatgatca
    38041 ttccatcagg tacagcttcc cagcgacgta tcagtcaccg tagacattaa agtcgaaata
    38101 ctttttcgcc atcttgtcgt aggtgccgtc ctgacgcagc tcgccaagcg ccttattgaa
    38161 ggcagccgtc agttcagcat aatctttacg tagccctaca ccggtgccat caccgaagta
    38221 ttttttgtct tttactgatg agccagcaaa agcgaaatct ttaccagcag gttgcttgag
    38281 gaatccttcg ctggcagcaa cttcatcttg taacgcagca tccagacgtc ctgcagccag
    38341 atcggaatag accaaatcct ggttggcata ggccaccaca tccacgcctt tactacgcca
    38401 ggtctcgtta gcgtaagctt cctgggttga tccctgcagc acaccaacat gtttaccttt
    38461 cagtgaatcc agcgttggct gaatcggtga acctttggca gcaatcaaac gagaatctgc
    38521 ggcgtacagc ttgtcggaga aggcaatctc ctgctgacgt ttatcggtaa tggaaagcga
    38581 cgaaataata gcgtcgattt ttttcgcttt cagtgagggg atcagcgcgt caaagtcact
    38641 ggcaacccag gtacatttca cctgcatccg tttgcacatc tcgttaccga gatcgatatc
    38701 aaagccaaca aaatcacctt tagcatcttt cgacgagaac agcgcatagg tggtatcggt
    38761 tccgatacgt accgtctccg gtagcgccgc atagctggaa gccgctgcgg agagaccgac
    38821 taataaagac agagcgagaa tcgacttctt catacataac cctcaagtga aaatggcttt
    38881 tttatgttgt gtattgtgtt gtgtgtttgc aggctctttc atgcaggtct tatgccatct
    38941 tgacgacaac agtaacattc aacgttaaat atgttaataa gacattgcat tattgtcctg
    39001 aagttgaaga tagcaggtat agcggttgaa tcgcagcgtt tcgattgaat ggcagaaaca
    39061 aaatgtcgag gatttgatcg cggttgcaaa attgccctga aacagggcaa cagcggagtt
    39121 atgcgccctg ccagcgggca aagagatctt caggaagggt tattgcaaac tggtcaagaa
    39181 cacgattaac cgtctgattt atcacatcat caagggattg cgggcgatga taaaacgccg
    39241 gaacgggagg cataatcacc gcaccgattt ctgccgcctg agtcattaaa cgcagatggc
    39301 ctaagtgcaa tggtgtttca cgcacgcaga gcaccaacgg gcgacgctct ttcagcacca
    39361 catctgccgc acgggtcagt aagccatcag tatagctatg gacaatgccg gacagggttt
    39421 tgattgaaca gggtaaaatc accatcccca gcgtctggaa agaaccggaa gagatgctgg
    39481 cggaaatatc gcgcgcatcg agcgtgacat cagctaatgc ctgcacttcg cgcagggaaa
    39541 aatccgtttc gagggataag gtctggcgcg ccgcctggct catcaccaga tgcgtttcga
    39601 tatctgtgac atcgcgcaga acctgtaata agcgcacgcc ataaatcgcg ccgctggcac
    39661 cgctgatgcc tacaatgagt cgtttcataa atagttgccc tgtcagactt gcaggcagac
    39721 tttgcagtat ttcggcggga gttgcaagtc agggtgccag accggcaccc tcagcgaagg
    39781 catcatcctt cgttatgcat ttcgagattt tccacttcgt tctgacgttg cactgctttg
    39841 gcgtcatcat tacgtaacgt atcgaggaaa tcgaggtagc cctgatcaac atctttggtg
    39901 acgtagacgc cgttgaacac cgagcattca aactgctgga tatccggatt ttcagcgcga
    39961 acggcgtcga tcagatcgtt cagatcctgg aaaatcaacc cgtcagcacc gatgatctgg
    40021 cgaatttcat caacttcgcg accgtgagcg atcagttccg tggcgctcgg catatcaata
    40081 ccataaacgt tcgggaagcg aatttccggt gccgcagaag cgaggtacac tttcttcgct
    40141 ccggcttcgc gtgccatctc gataatctgc tcagaagtgg tgccacggac gatggagtcg
    40201 tcgaccagca ggacgttttt atcgcggaac tcggcgcggt tggcattcag tttacggcgc
    40261 acggacttac gacgcagctg ctggcccggc atgataaagg tgcggccaac atagcggttt
    40321 ttaacgaagc cctggcggta cggtttgccc agaatacgag caatttccag cgcgatatca
    40381 cacgaggttt ctgggatcgg gatcaccacg tcgatatcca gatcttccca ttcgcgggca
    40441 attttctcgc ccagtttcgt gcccatattc acacgcgcgc tgtaaacgga aattttgtcg
    40501 ataaacgagt ccgggcgggc aaagtataca tactcaaaca ggcacggatt gctgaccgga
    40561 ttgtcagcac attgacgggt aaacaactgc ccttcttcag tgatgtaaat cgcttcgccc
    40621 ggcgcgacgt cacgcaggaa atcaaagccc agcgtatcga gcgctacgct ttcggaagcg
    40681 accatatatt ctgtacggtt ctcgtcaata tcacgttttc ccagtaccag cggacgaatc
    40741 ccgtttggat cgcggaaagc aaccataccg tggccgataa tcatcgccac acaggcatac
    40801 gcgccgcgga ttaagcggtt tgtggcagca atggcagcga aaatattgtc ggcttccagc
    40861 gggtagtggc ggaagttgtc cagctcgctg gcgaagatat taagcagaat ttccgagtcg
    40921 gaagtggtgt tgatgtggcg gcgtttttct tcaaacagtt ttttacgcaa ctcgtgagcg
    40981 ttggtcagat tgccgttgtg ggcaagcgta atgccatacg gggagttaac gtaaaacggc
    41041 tgcgcttcag aggcgctgga gctgccagcc gtagggtaac gcacatgacc aatgcccata
    41101 ttgccctgca aacgctgcat atggcgagct tcaaatacat cgctcaccag cccgttcgct
    41161 ttacgcaaac ggaagcagtt attggcatct atggtgatga tgccggcggc atcctgaccg
    41221 cgatgctgaa gcaccgttaa ggcatcataa atcgactggt taaccggcat aacaccggcg
    41281 ataccgacaa taccgcacat acgtcttttt cctcgttaag ccacatctca gagcacttac
    41341 gctctgggca agaaacttga cgagctttgc agataatcaa aaaaccatct gatgataaaa
    41401 ctgaattgcg ggatcagctg tgatttgctc cagtcttcac ttttcgacac cccggtaaag
    41461 gagtcgagaa agaagagaat ggcggcaaca atcaacacac cgcgcaacgc accgaaacag
    41521 acgcccagca cccgatcggt gcctgacaac cccgttttct ccaccaactg gcctatcacg
    41581 aagttcacga tagcaccaac gatcagggta gcgataaaca gtaccgcgat ggcaatccca
    41641 tttcgaacca gttcgtcttc aaagcccgta aaccagactg acaggtaagt gtagtaatga
    41701 ctggcaacaa agaaagcaca accccatgtc accagcgata acgcttcacg aacaaagccg
    41761 tggatcaggc taaccagaga ggaaaaagca atcaccgcga ttatggcgta atcaatccag
    41821 accatatgtg tcccacgatt ttacgccctg tcatcctgtt cggggcgcat tctaacagaa
    41881 aaagaaaacg tttgcgtagg gatttccttc ccgcgcatca ataaaaatgg cgctgaaaaa
    41941 atattcaacg ccatcgactt tttatgcctt tgcggcatcg tgcaatgcgt gtcggatgcg
    42001 gcgtaaacgc cttatccgac ctacggttct aacactgccc aagcctgata agacgcgcca
    42061 gcgtcgcatc aggcaagact gtattaattc ggcgtatagc ccattaccac gccacttaag
    42121 ccagaaagtt gcttcaactc acccagcgaa cctttcagct tatctttcga ggcatccggc
    42181 ccaaccagaa tacgggtaat tttaccctgc actggcgtgg atggcgacgt ataaacccga
    42241 taaccggcac cgcgcagctt accgacaatc tcattcactt tatcggcatt tttcagcgca
    42301 cccagttgca caacataggc tttacccgtt ggcgctgctt tttcttccac gaccggcttc
    42361 ggttctggct tcggcgcagg tggtacttca acctttggtt taggcggctc caccggtttc
    42421 ggcttcggtg gggcgaccgg tgcaggttcc ggttcaaact cggtgttatt ggctgcaata
    42481 gtggccggat cgagcgacgg tgctgcggca tcacctgccc gcacctcttc cgctgcgcct
    42541 tccggcggct gcgtcggcaa cgcctgggtg gcggctggca tcatatcagg ctcatcacga
    42601 tcgcccgctt tcggcaccag cgggatagcc gcgaactcat cctgataatg ttttttctgc
    42661 ccgtccagca gccctggaag tacaatcacc cccagcgcca ccagcacgat cgtgcccact
    42721 aaccgattct gaaacttact tgccaccgct tctcctcgcg tcaatcactt ccatgacatg
    42781 tgcgaccgtg tggaaagaac cacacaccag cacggtgtct tccgctttag cgtccgccat
    42841 tgcggcatcc catgcctgcg caacgctatc aaatgatttg ccgttaccca aatgctcaag
    42901 cagttgttct gccgtggcac cgcgcggccc ttccagtggc gcacaatacc agtcatcaac
    42961 cacgcttttc aaccaggcca gagttccggc aatatcttta tcatgtagca taccgataac
    43021 cgccagcacg cgcccgtttt tcggtagcgc tttcatacgc ccggtgagat attccgccgc
    43081 atgtggatta tgcgcgacat caaaaataac gcgtggcgac tcgctcacaa tctggaaacg
    43141 tcccggcaaa attgcgctgg caatcccgtc gcgaatggca ttttcactga cttccagccc
    43201 gctggcacgc agtgccgcca gcgccgttgc ggcattcggt tgcgggacaa gcggcaacgg
    43261 cagattttcc agcgtgccgt gagcatcgct aaacgcccag tcatgatcgg tgacggaata
    43321 gttccactca acgccccgac gttgtaacag tgcacctttt tcctgcgcca catcagcaat
    43381 ggtagaaggc atttccggct caccgacaat tgccggtttt tcgctgcgga agatgcctgc
    43441 tttctcgaga ccaatacttt cgcgatctgg acccagccag tcggtatgat ccagcgcaat
    43501 actggttact accgcgacat cggcatcgac aatattggtt gcgtccagac gaccgcccag
    43561 ccctacttcc agaatcacca cgtcaagttg tgcctgcttg aacagccaca acgccgacag
    43621 cgtaccgtac tcgaaatagg tcagggaaat atcaccgcgt gccgattcaa tctccgcaaa
    43681 agaggcggtg tgggccgatt ccggcaattc ctggccctgc acacgtacgc gctcggtata
    43741 acgcaccaga tgaggcgaac tgtagacgcc cactttgtac cctgccgcca tcagaatcga
    43801 ctccagcgta cggcaggtgg tgcctttgcc attcgtaccc gcaacggtaa acacaaatgg
    43861 cgctggtttc aggacgccaa gacgcgccgc gacctggctc acgcgctcaa ggccgagatc
    43921 gatagtttta ctgtgcaggt tttccagata agaaagccac gaagccagag gcgacgcggc
    43981 ttgaggagtg cgtttgataa tcatggtatc cgctgattcg ttacggtgag aatagcaaaa
    44041 gggcagagcc agtggccctg cccttatcag ttatcaggcc tcaggttcct gatccggtac
    44101 cgggggtacc actacgcctt cacgcggcgc ttcaggattc ggcgctggca gattcatcaa
    44161 cttcgccaga atgctcgcca gtttcaggcg catttccgga cgacggacga tcatgtcgat
    44221 cgcgcctttc tcgatcagga attcactgcg ctggaatcta ggcggcagtt tttcgcgaac
    44281 ggtctgttcg ataacacgcg gaccggcaaa gccgattaac gctttaggtt cagcgatgtt
    44341 gagatcgccc agcatggcga aacttgcaga aacaccgccc atcgtcgggt cggtcagcac
    44401 ggagatgtac ggcaagccgc gctcctgcat ttttgccagt gccgcagagg ttttcgccat
    44461 ctgcatcagc gacatcagtg cttcctgcat acgtgcgcca ccagaggcgg agaagcagat
    44521 cagcgggcag ttatcttcct gcgcctgctc aacggcacgc acgaaacgtg cacccacaac
    44581 agaccccatt gaaccgccca taaaggcgaa ctcgaatgcc gcagcgacaa ccggcattcc
    44641 atacagagtg cctttcatca ccaccagcgc atctttttcg ccggtttctt tctgcgcaga
    44701 tgccagacgg tctttatact tcttggagtc acgaaacttc agctcatctt tcggctcaag
    44761 ctcgctaccc agctccacaa ggcttccttc atctaacagg ctatgcaggc gattacgcgc
    44821 tgtcatacgc atgtgatggt cacacttcgg acagacctca agattacgtt ccagctcagc
    44881 gcggtataaa acctgaccgc agctatcaca cttagtccac accccttcag gaatgctcgc
    44941 cttgcgggtg ggagtaatgt tgcttttaat tcgttcaatc cagctcatta gggacctttc
    45001 tgtctgaacc tggttcgatg ccagttttat ctttggggac gcataatgcc atttttgccc
    45061 ccaacagacc atgaatgttg cacattaaaa cataacagcc cgaaactttg gataaaaaag
    45121 tggtcgaacc gcggagttac tttttatttt gcggcgcgtg ccgcagcgcg tttgtgacgg
    45181 attatttcga tgacgcccgg caaaatagaa accacaataa tcccgacgat cagcagctta
    45241 aggttatcct gaaccatcgg gattgtaccg aagaaatagc ctgcgtaggt aaaaagcagt
    45301 acccacaaca gtgcgccgat cacgttataa gcagcgaaat gacggtacga catgtggccc
    45361 attcccgcaa caaacggggc gaacgttctg acgatcggca caaaacgggc gagaataatc
    45421 gttttgccgc catgtttctc ataaaactga tgggttttgt cgagataact gcgacggaaa
    45481 attttcgaat tgggattact gaataacttt tcaccgaaca accgcccgat agtgtaattg
    45541 accgcgtcac ccacaatcgc ggcgatcagc atcagcacta ccatcatatg gacgttgaga
    45601 tcgttggttt ccagtgatgc caacgctccg gcaacaaaca gcaatgaatc acccggtaaa
    45661 aacggcatta ccaccagacc ggtttcacag aacaaaatta agaacaaaat ggcataaacc
    45721 cagacgccgt actccgcgac tagctctgcc agatgcacgt caatgtgcag gataaaatcg
    45781 atgaggaaat aaatcaggtc catatttgcc tatgccttgt actcgtcatc tttcaggctg
    45841 taactacgtt ggctacagcc tgaaatgttc cgagcattat tctcgttagt ccgccagaaa
    45901 tagcgggccc attggcggtt ttggaagatc ataccggtca gggtaatcca ccgcgaccag
    45961 atacagccct tccgcttttg ccgttgctgc cgccagcgtt ctgtcctttg ccgccagcaa
    46021 ctctgctatc cagctctccg gctggttgtg ggcaccgact tccatcaggc tgccgacaat
    46081 attcctgacc atatgatgta caaaggcatt cgctttgata tcaaccacca cataaggacc
    46141 gtgacgcgtg acattaatat gcataacgtt gcgccacggg gttcgggact ggcactgcac
    46201 cgcacggaac gaggtgaaat cattctcgcc cagcaagcat tgcgcagccc gatgcatccg
    46261 ttcagcgtcc agcggttcgt aaaaatgggt taccccttta ctcagtaccg ccgggcgcag
    46321 ccgatgattg tagatgatgt agcgataacg gcgagccgtg gcgctaaatc gggcatgaaa
    46381 atcatcaggt acagctttaa cccaacgcac ggcgatgtca ccaggtaaat tcgcatttac
    46441 gcccaaggtc cacgccgcgt ctttgcgctg cgcggttgtt tcgaaatgca ccacttgccc
    46501 ggtaccgtgt acccctgcgt cagtacgccc ggcgcagaag acggtgatgg gttcgttcgc
    46561 cacctgggag agcgcctttt ccagcttctc ctgcacactg cggacttcat tctgccgttg
    46621 ccagccgtaa tacttactgc cgtcgtactc aatgcccagc gcaattttat aaactggcag
    46681 ttgttgctgg tcggacatta gtacagatac tcctgcacca gtttctcggc gatttttact
    46741 gccatcagcg cgccgccaaa gcgaacgtta tcggccaccg accagaactg gacttgctcc
    46801 ggcataccgt agtcattacg cacgcagcca acagaaagat gcggcgtacc cgaagcatcc
    46861 cctacctgag tcgggaattc gttctcttca gagagcacaa tatcttcgcc ctgagcaaac
    46921 gcatcacgcg cttcttctgc cgccagcgga cgcagtgctt caaagttgac catctgggca
    46981 tgaccgtaga ataccggtgc ctggacgacg cttgccgaaa tcatcagccc ttcgtcctgc
    47041 aggattttgc gtacttcgtc aacgatacga cgttcttcac gcacgctacc ttcgctatcc
    47101 ggcaacaacg gcagcatatt gaacgccagt tgacgaccga agaaatcttc ttcgtcaatc
    47161 ggaatgccgt tgagcaattt cgcactctgc cccgctaacg catcgaccgc ttttttgccc
    47221 tgggcggagg ctgaaatcag gctggtaaca ctgatacgcg acaaaccgcc ctgatcgatt
    47281 aacggtttca gtgccgccag cagctggctg gtcagactgt ctggtacggc gatgacattc
    47341 cggttgcggt aatcggtcag tacaaacggg tttacttccg gcaccaccag cggtacgtcg
    47401 ggttcgagag caaacagtcc gctgctgtcg atcaccaggc aacctgagtt ggtcgcttct
    47461 tcaacccagg cagcggtagc ttctttgcct gcgacaaaaa atgccagctg cgcctgcgtc
    47521 cagtcgaatt cagcggcatc ctgcacggtg attgttttac caccaaagcg cagttgttcg
    47581 cctgcgcttt cgttacgtgc cagtgcataa atttccccaa ccgggaactg acgttcagcc
    47641 agcgtttcaa gcagggcttc gcccatagcg ccagttgcgc ccaggacggc aatgttccag
    47701 ccttcagaca tggtggttta ctccagaaat agcaaagctc cctgccaatg ttacagcaga
    47761 gagcatgaag aagagattaa cgtgccggat gatgaacggc gttaaaaccc agtttacaca
    47821 gcaatgatgc cgcactggcg tcatcacaaa ttacatacag agatgaccat tcacggcgct
    47881 caagatagtt tttgcgcagt ttatcgaact cacccggtat cccggcgact ttacgcagcg
    47941 gtgcgtcatc gcggcgcaca tcatacacca aatgcaccag ccttttcagc gtcggttgat
    48001 cgagcggacc atgcagcgta atgcggccaa actcaggcgc gggcagcaac gtatccagcg
    48061 caacgtgttg ttcatgccca ataaacttgc tataggcttc aaacacttgc gtcgtgccac
    48121 gcgctttacc ctccagggta tagcctgcga tatgcggcgt gccgatatcc acttttgtca
    48181 gcaattccac attaagttcc ggctcgcctt cccagacatc cagcaccacg cttaacttct
    48241 ggccttcatt caggcaggtc agcagcgcgg tattatcgac gactgcgcca cggcaggcgt
    48301 taatcagaat cgctccgggc ttcaggctac ggatcagttt ttcatccgcc agatgtagcg
    48361 ttttgtacgg accatcttta aagagtggcg tatggaaagt cagaatatcc gcgcgctgga
    48421 ctaactcatc cagcgagcgg aaatcaccct catccccacg gtcggcgcga ggcggatcgc
    48481 aaagtaaggt tttaatccct aacgcttcca gtcgcgcctg caatcgacgt ccaacgttac
    48541 caacgcccac gatccccacc gtacggtcgt gcagtgaaaa tccatcgcgt tcggcaagca
    48601 tcagcaggga ggagaaaaca tattccacca ccgcaatcgc attacagcca ggtgcagcgg
    48661 aaaaaccaat tcccgcctgc ttcaaccatg cttcatcgac atggtcggtc cccgcagtgg
    48721 cagttccaac aaatttaatg ggttttcctg ccagcaaaga ttcattcact ttcgtgaccg
    48781 aacgcaccat cagcgcatcc gcgtctgcca gttgagcgac ggggattgga cgcccgggaa
    48841 ccgcgatcac ctcacccaaa cggctaaata agtcgcgggc ataaggcata ttttcatcaa
    48901 caaggatttt cacgtttgtg ttacctgtat gagacgagag ttaaccggac aagtatgcca
    48961 taatctcgcg gccaggcata cttgcgaaga tttcaggtat aaggatacgt aatgatacaa
    49021 cctatttccg gccctcctcc tgggcagcca ccaggtcagg gagataacct gccgtctggc
    49081 gcgggcaatc agcctttatc cagtcagcaa cgtacttcgc tggaaagctt aatgacgaaa
    49141 gtgacctcac tgacgcaaca acaaagagca gaactgtggg cgggtatcag gcacgatatt
    49201 ggtctgtcgg gagattcacc gctgctttcg cgtcacttcc ctgccgctga gcataatctg
    49261 gcgcaacgtc tgctggccgc gcaaaaaagc cattctgccc gccagctttt agcgcaatta
    49321 ggggagtatt tacgtctggg gaatcatcgt caggcggtca cggattatat ccgtcataac
    49381 tttggtcaga cgccgctgaa tcagctctca ccggagcaat taaaaaccat tctcaccctg
    49441 ttgcaggaag ggaagatggt tattccgcaa ccacagcagc gcgaggcgac cgaccgtcct
    49501 ttattaccgg cggagcacaa tgcgctaaaa cagctggtga ccaaacttgc ggcggcaacg
    49561 ggggaaccca gcaaacagat ctggcaatcg atgctggaac tttccggggt gaaagatggc
    49621 gagttaattc cagcgaaact gtttaaccat ctggtgacct ggctacaggc gcgtcagacg
    49681 ctaagccagc aaaatacgcc gacgctggaa tcactacaga tggcgctaaa acaaccttta
    49741 gatgccagtg aactggcggc gttatcggca tatatccagc aaaaatatgg cctttctgcg
    49801 caatcatcgc tttcttctgc ccaggccgag gatattctta atcagcttta tcaacggcgg
    49861 gttaaaggga ttgatccgcg tgatatgcaa ccgctgctta atccttttcc accgatgatg
    49921 gacacgttgc aaaatatggc aacgcgtccc gcgctgtgga tactgttagt cgcgattatc
    49981 ctgatgctgg tctggctggt tcgttaaccc cgacgaaatg acagtatcgt gacaataata
    50041 cccagcaccg cagagatcgc cccggcaaga aataccgaag agtaaccaaa cgtggtcgcc
    50101 agcattcccg ccagcggccc ggagacgccg agggcgatat cctgaaacgc ggcgtaaccg
    50161 cccagtgcgg tgccgcgaac ttgtgagggg acgcgtttaa ccacctccac gcccagcgca
    50221 ggaaagataa gcgaacatcc ggctccggtt aacgccgcgc ccgctaatgc gacccatgca
    50281 cctggggctt gccagagcag caacaagccc accgtttcta caagcagaga gacaatcgcc
    50341 actttcacgc cgccaaaacg gtccggcatc cagccaaaca tgacgcgcat cacgacaaat
    50401 gcgccgccaa acgcggtaag agtaaagccc gccatcgccc atcctttgct ggcaaagtag
    50461 agcgaaacga aagtcccgat aaccgcaaaa ccaacgcctt gtagtgccag ccctaaccct
    50521 ggtttccaga taagcccgac aacgctccac agcgatggac gttctcccgc cagggccggt
    50581 actttgcgca ctgtgccgtt acaggcccac gccagtaagg gtaataccat tgtggtgatc
    50641 gccagtgcgg caaaaccgta atggctatga atcaacaggc caagcggcgc accaacagcg
    50701 agggcaccgt aaatcgccat tccgttccat gacatcactt tgccagagtg ttttggccct
    50761 acgatgccta acccccaggt cagagcgcct gtcagtaact ggctttcacc aaacccaaga
    50821 atcaaacgcc cgatgaccaa cagggcaaat ttgaacggtg cggagacagg caaaatcgcc
    50881 gccagcagca acgcgccgcc agccagacca caagctaaca tcccctgaag cgccgaacgt
    50941 tttgcgccat attgatcggc cagtcgcccg gcgtaaccac gcgtcagcac cgtagccaga
    51001 aactgaatcc cgacggcaat gccgaccatg gtattgccat agcccagatc atgatgaaca
    51061 aacagcggga taaccggcaa cggcaaccct acggtcatgt aggtgagaaa aaccgcaaaa
    51121 gagatgcgga agagcgaaaa actggcagaa gatcgtgttt cggtttggct tacagcagtc
    51181 atgcattact ccagaatgca gcgcaaggcg aggagtatcc ccgtctcatc tctctggttt
    51241 cagggttacg gtgcgttggc aggatttaac gcgtacgtct tttcagaagg aaatcgacaa
    51301 agcgggaagt ttgcctagaa ctggtggcga ttgtcaatga tgtgaaaaag ggaaccatca
    51361 ggttcccttt tgcgttagtg ccggaggccg cattggcgcg taacgtcgga tgcgacgctg
    51421 gcgcgtctta tccgacctac tccggcctac tgcgaattaa tcttttcagc ttgcgcatta
    51481 ccagcgtggc gttggtgccg ccgaagccga agctgttaga cataacagtg gtcagttcgc
    51541 gatcggtcgt ttcggtcacg atgttcagac ccgcagcctg ctcgtccagc tcttcaatgt
    51601 tgatgctcgg ggcgataaag ccgtgttcca gcatcagcag agagtagata gcttcctgca
    51661 cacccgcagc gcccagtgag tgaccggtca tggctttggt tgcagaaatc gccgggctct
    51721 tatcgccgaa cacttcacgg atagctgcca gctctttcac gtcgccaacc ggagtcgaag
    51781 taccgtgaga gttcaggtaa tcgattgggg tatcaacgcc atgcatcgcc atctgcatac
    51841 aacgtactgc gccttcgcca gacggagcaa ccatgtctgc accatcagag gttgcgccgt
    51901 agccaacgat ttcagcatag atgtaagcac cacgcgccag cgcgtgttcc agctcttcaa
    51961 ccactaccat accgccgccg ccagcgataa cgaaaccgtc acggtgagcg tcgtaagtac
    52021 gggaggcttt ttccggggtg tcgttgtatt tagtagatag cgcacccatc gcgtcgaatt
    52081 cgcaggccat ttcccagcac agctcttcgc cgccgccagc aaacacgatg tcctgtttgc
    52141 ccagttggat ctgctctact gcgttaccga tacagtgtgc ggaagtcgca cacgcggagc
    52201 tgatggagta gttaacgcca tgaattttaa acggcgtggc gagacaggca gaaacgccgg
    52261 atgccatcgc tttagtgacc acatacgggc caaccgcttt caggccgcgc gggccgcgca
    52321 ttgcgtcagc gccgaacacc tggaaacgcg gggaaccgcc gccggaacct gcaatcaggc
    52381 caacgcgcgg gttattctgg taagcttccg gagagaggcc cgcatcagcg attgcctgct
    52441 ccatagaaag gaatgcataa atggatgcgt cgctcataaa gcgcacaact ttgcggtcaa
    52501 tgaggccagt ggtatccagt tttatgttgc cccagacgtg gctacgcatg ccggaatcct
    52561 tcagctcctg agagaaagtg atccctgaac gtccttcacg cagagatgcc aggacttcct
    52621 gctggttatt accgatgctg gaaacaatgc ccaggccagt aatcactgca cgtttcattc
    52681 aatacctctg taagtcgcac atagagtaag tttcgaatgc acaatagcgt acacttgtac
    52741 gccgaacaag tccgatcagc catttaatag agatatttgc gcagccttac acacatcgct
    52801 aagatcgagc caccgcctgt aagacgagta acttacgtga aacactactc catacaacct
    52861 gccaacctcg aatttaatgc tgagggtaca cctgtttccc gagattttga cgatgtctat
    52921 ttttccaacg ataacgggct ggaagagacg cgttatgttt ttctgggagg taaccaatta
    52981 gaggtacgct ttcctgagca tccacatcct ctgtttgtga tagcagagag cggcttcggc
    53041 accggattaa acttcctgac gctatggaag gcatttgatc agtttcgcga agcgcatccg
    53101 caagcgcaat tacaacgctt acatttcatt agttttgaga aatttcccct cacccgtgcg
    53161 gatttagcct tagcgcatca acactggccg gaactggctc cgtgggcaga acaacttcag
    53221 gcgcagtggc caatgccctt gcccggttgc catcgtttat tgctcgatga aagccgcgtg
    53281 acgctggatt tatggtttgg cgatattaac gaactgacca gccaactgga cgattcgcta
    53341 aatcaaaaag tagatgcctg gtttctggac ggctttgcgc cagctaaaaa cccggatatg
    53401 tggacgcaaa atctgtttaa cgccatggca aggttggcgc gtccaggcgg cacgctggcg
    53461 acatttacgt ctgccggttt tgtccgccgc ggtttgcagg aagccggatt cacgatgcaa
    53521 aaacgtaagg gctttgggcg caaacgggaa atgctttgtg gggtgatgga acagacatta
    53581 ccactcccct gctccacgcc gtggtttaat cgtacgggca gcagcaaacg ggaagtagcg
    53641 attatcggcg gtggtattgc cagcgcgttg ttgtcgctgg cgttattacg gcgcggctgg
    53701 caggtagcgc tttattgcgc ggatgaagcc cccgcgctgg gtgcttccgg caatcgccag
    53761 ggggcgctgt atccgttatt aagcaaacac gatgaggcgc taaaccgctt tttctctaat
    53821 gcgtttactt ttgctcgtcg gttttacgac caattacccg ttaaatttga tcatgactgg
    53881 tgcggcgtca cgcagttagg ctgggatgag aaaagccagc ataaaatcgc acagatgttg
    53941 tcaatggatt tacccgcaga actggctgta gccgttgagg caaatgcggt tgaacaaatt
    54001 acgggcgttg cgacaaattg cagcggcatt acttatccgc aaggtggttg gctgtgccca
    54061 gcagaactga cccgtaatgt gctggaactg gcgcaacagc agggtttgca gatttattat
    54121 caatatcagt tacagaattt atcccgtaag gatgactgtt ggttgttgaa ttttgcagga
    54181 gatcagcaag caacacacag cgtagtggta ctggcgaacg ggcatcaaat cagccgtttc
    54241 agccaaacgt cgactctccc ggtgtattcg gttgccgggc aggtcagcca tattccgaca
    54301 acgccggaac tggcagagct gaagcaggtg ctgtgctatg acggttatct cacgccacaa
    54361 aatccggcga atcaacatca ttgtattggt gccagttatc atcgcggcag cgaagatacg
    54421 gcgtacagtg aggacgatca gcagcagaat cgccagcggt tgattgattg tttcccgcag
    54481 gcacagtggg caaaagaggt tgatgtcagt gataaagagg cgcgctgcgg tgtgcgttgt
    54541 gccacccgcg atcatctgcc aatggtaggc aatgttcccg attatgaggc aacactcgtg
    54601 gaatatgcgt cgttggcgga gcaaaaagat gaggcggtaa gcgcgccggt ttttgacgat
    54661 ctctttatgt ttgcggcttt aggttctcgc ggtttgtgtt ctgccccgct gtgtgccgag
    54721 attctggcgg cgcagatgag cgacgaaccg attccgatgg atgccagtac gctggcggcg
    54781 ttaaacccga atcggttatg ggtgcggaaa ttgttgaagg gtaaagcggt taaggcgggg
    54841 taatctgctc tcgcagtgtt tgccggatgc ggcgtgaacg ccttatccaa cctacgtgtg
    54901 acgtgtaggc ctgataagac gcgataagcg tcgcatcagg catggtgctc cagacgccgc
    54961 aacttacagc tgtgacgcct gctgaaacag gttttcccac atatcggtca ccagcgcctg
    55021 gtcacgcggc gacaattcac cggcaccaat ggctttttcc agactctggc taacggtcgt
    55081 atgcaccgcc tgagcggagt ggtcatcacc actttccagt tctgcgatgg ctaatgtcag
    55141 gtggccacgc aaatacccac tggcaaacaa ctcatcatca ctggcatggt caaccatgcc
    55201 gtcgattaat gccagaatgc gtgattcaaa ctccgcgatc atcttctttc ctcattgtaa
    55261 aacgtggcgc tggcttcagt tgagcgcttc cggccacggg aactgttccg ccgtaagttc
    55321 cggcgtgtga taataatttt gtagtgcttt aatgaagcgt gccgggcgtt ccggaatgcc
    55381 atttgtcaga taatccatca cctgcgcatg tacccgccgt tgaaacacca cacgatccgg
    55441 ttcgaaatcc ccttccagat tgtcgcaact gacgttaaac ggatatccag ccgccacgca
    55501 gaataaccag tcgaacgcct gcggcttcac ttcaacatct tcaaactggc tttgcgtctg
    55561 ggcatcgcgt ccgtccgggc aataccagta gccgaaatca accagctcac ggcgcgcttt
    55621 tccggcaata caccagtgcg aaatctcatg aattgcgctg gcataaaagc catgagcaaa
    55681 gacgattcgg ttatacggta cttccgcatc agcaggaaga tagatcggtt cgtcgtcgcc
    55741 tttaatcaga cgggtattaa attcatcggc aaagcagcta ttaaaaattt caatcaactg
    55801 ctcgtagtgg tgtgtactgt tcattagttc atccccaacc agtggaggat ctcctgtccg
    55861 tggctatcgt aaagtaattt ggcactcatc accgccgaga cgataacaat catcgggcgg
    55921 atcagctttt gtcctttgct caacaccaac cgtgaaccca tgcgcgcgcc gaggaactgc
    55981 cccaccagca tgacaaagcc cgtcgcccaa atcactttgc cgccgagaat aaacagcagc
    56041 agaccgccaa tgtttgacgt tgcgttgagt aatttggcat gagccgtggc tttggcgagg
    56101 ttgaatccgc acagcataac gaaggccagc gcgtaaaacg atccggcagc cgggccaaag
    56161 aatccgtcat aaaaaccgac gcagccaccg gcaattaacg cgaagggtaa accgtacatc
    56221 cggcgctggc gatcttcttc acccagcttt ggcatcagca agaaatagag gccgatacaa
    56281 atcaccagaa tgggcaaaat ctgccgtaag acatcagcct gaacgtactg taccaacagt
    56341 gcgccgctca ttgagccgac aaaggtcatg gcaatattga gtttctgatc gcttaaacta
    56401 accactttgc ggcgaataaa gtagatagtg gcggaaatag agccgccgca ggcttgcagt
    56461 ttattggttg ccagcgcgtt agcgggagac attcccgcag ccatcaatgc cggaatggtg
    56521 agtaacccac cgccaccggc aatcgagtcg ataaatcccg ccagcatggc gacaaaaaag
    56581 agaactccca gcaacagcgg agaaaccata aacaggctat taaacgtttc cattagatca
    56641 cgtgctcatc cagtagcgcc tggcaggaag gcggcaacgg aggcggtgtc ttcttctcag
    56701 gctttgttgt tcccggtttt ggaggttcaa accagctttg cagttctgcc ccgcaaccat
    56761 cgcctgatgg cggtaaaggt tgatcttcac actccagact atcggcaggg caacgtaatc
    56821 gtacatgcat atgcgcgcga tgctggaacc agggtcgcac tttgcgcaac cagtcgcgat
    56881 cggtgcccgc atcaaggcaa agttgttgtt taatcgccgg attaacaaaa atgcgcgtga
    56941 cgtctttgtc ctgggcggcg agtttgatca agctgaaaat ttctggcttc cacagcgtgg
    57001 agacaacgtg tttaccgtcg cgggaaacta agtccagtgc ttgcgggcgc aagagctgcg
    57061 cggaggtcca gcgagttttc ggcagttgca gaaagatatc gacatccagt ccggtctggt
    57121 ggctggcatg accgccgttg aaacgcccac cagcgggcat ccccatatcg ccaatcagca
    57181 ccgtacccat gcccagattg ctcacctggc tactcagacg ctggataaac atcaccagat
    57241 ccgggtgacc gaaatagcga cgctgatcgg tacgcatgac ctgataatgt tcggactgta
    57301 tcggcaacgt gtcagcgccg acaatacagc cattagaaaa actgcctatc gattgtgcgc
    57361 tacccggcac aggttgggtt attttttgcc acggcgtcgc tgccaggcta gcactactgg
    57421 caagcagagc cagcagcgca atcgcggttt tgttcatttt ttaccagcgt ggaatatcag
    57481 tcttcacatc ggcattttgc gcccgctgcc gtaacaggtg atccattaaa acgatcgcca
    57541 gcatcgcttc agcgatcggc actgcacgga tcccgacaca gggatcgtga cggcctttgg
    57601 tgatcatctc aacttcttcg ccaaagcggt taatagtgcg ccccggcacg gtaatgctgg
    57661 aggtcggttt cagcgccata tgagcaatga tttgctgccc gctgctgata ccgccgaaaa
    57721 tgccgcccgc atggttgctc tggaaaccgt ctttggtgat ttcgtcgcgg ttctggctgc
    57781 cgcgcagcgc cactacgtca aaaccgtcgc caatttccac gcctttcacc gcgttgatgc
    57841 tcatcagcgc atgggcgatg tcggcatcca ggcggtcaaa gaccggctcg ccaagtccgg
    57901 cggggacacc actggcaaca acggtgactt tcgcgccgat ggagtcgccc tcttttttca
    57961 gcgcgcgcat cagttcatct aacgcgtcga ttttgtcggg gtccgggcag aagaacggat
    58021 tttgttcaac cagcgaccag tctttgattt ccagcggaat gtcgcccatc tgggtcaggc
    58081 agccgcggat ttcaatacca aatttctcgg cgagatattt tttggcaatc gcccctgccg
    58141 ccacgcgcat ggcggtttcg cgggcggaag aacgtccacc gccgcgataa tcgcgcagac
    58201 cgtatttttg ttcgtaggtg taatcagcat ggcctggacg gaagacgtct ttaattgcgc
    58261 tgtaatcctg agaacgctgg tcggtatttt cgatcaacaa gccaatgctg gtgccagtag
    58321 taacgccttc aaaaacaccg gagagtattt tgacctgatc cggctcgcgg cgctgggtgg
    58381 tatagcgcga tgtcccaggg cgacgacgat caaggtcatg ttgcaggtcc gcttccgtca
    58441 gcggaatgcc tggcggaaca ccatcgacga tgcagccgag cgccagcccg tgcgattcgc
    58501 cgaaggtggt tacgcgaaag agttgtccaa ttgtgtttcc agccatcacg gctccgttat
    58561 tgttgtgttt gcgtgtttac ttaatcttta taaatcgcga aatgttctcg tgcggcaata
    58621 agctgctctt tagtgagcat aaacacacca tcgccgccgt tatcaaactc cagccaggtg
    58681 aacggaacat cgggatattg ttccataaga tgtaccatgc tgttgcccac ttcacaaatc
    58741 aacacgccat catcagcaag gtaatctgcc gcgttaccga gaatgcgacg cgtcagtttc
    58801 aggccgtcgg tgccagatgc caggcccagt tccggctcgt gacggtattc gtttggcagg
    58861 tcggacatat cttccgcgtc gacatacggc gggttagtga caatcagatc gtattgcact
    58921 ttcggcaaat cacggaacag atcggaacga atcggaatga cgttgtggat cagaccgtgt
    58981 tcttcgatgt tctgttcggc aaccgccagc gcgtcgggag agatatctac cgcgtcgact
    59041 tccgcttccg ggaaggcata agcacaggca atggcgatgc agccgctacc ggtacacata
    59101 tctaaaatat gctgcggttg cttgctgatg agtccggcaa atttattgtt gatcagttca
    59161 ccaatcggcg agcgcggcac cagcacgcgt tcatcgacgt aaaattcatg accgcagaac
    59221 caggctttgt tggtcaggta agccaccgga atgcgctcgt tgacgcggcg gatcacgcgt
    59281 tcaacaatac ggtgtttttc gctggaggtc agacgcgcgg tgcgcatatc ttccgggata
    59341 tccagcggca ggtagagcga tggtaacacc agctgtacgg cttcatccca cgggttatcg
    59401 gtgccgtgac cgtaccagat atttgccgcg ctgaagcggc ttaccgacca gcgcaatatg
    59461 tcctgaatgg tttgcagctc atttactgct tcatcaacga aaattttatc cacgtattcc
    59521 tccagggcat gatcgcaata atttcggcgg ctagtttgcc atgaagatga cgataaatca
    59581 gcattcacgc gcggtgagtg aggaaaaata cgtttaaaac gatcgattgt gctacgagtc
    59641 gggtaaactg taggaaaatt agaaatagag acagataaat gaaaaagaaa acaacactta
    59701 gcgaggagga ccaggctctg tttcgccagt tgatggcggg gactcgcaag attaagcagg
    59761 acacgattgt ccaccgaccg cagcgtaaaa aaatcagcga agtgccggtg aaacgcttga
    59821 tccaggagca ggctgatgcc agccattatt tctccgatga gtttcagccg ttattaaata
    59881 ccgaaggtcc ggtgaaatat gttcgcccgg atgtcagcca ttttgaggcg aagaaactgc
    59941 gccgcggcga ttattcaccg gaattgtttt tggatttaca cggtctgacg cagctgcagg
    60001 ccaagcagga actgggcgcg ttgattgccg cctgtcgccg cgaacatgtg ttttgtgcct
    60061 gcgtgatgca tgggcacggg aagcatattt tgaagcaaca aacaccgctg tggctggcac
    60121 aacatccgca tgtgatggca tttcaccagg caccgaaaga gtacggcggt gatgcggctt
    60181 tgttggtgtt gattgaagtg gaagagtggt tgccgccgga gttgccctga atgctggcag
    60241 caaatgccgg agcagcgtaa acgccttatc cggcctacaa atatgtgata attcaataaa
    60301 ttgcaattca cttcgtaggc ctgataagac gcatcagagc aatactacga acttcagttc
    60361 attcaataca tactgaaaac gacacgtcga atcaatcggt tgcccactac tggtattcac
    60421 attcaccaca atatcgtgta gtactttatc cgttaccgga tgacgagttg gcgtaaccgt
    60481 taccgttgat ttatcacaac cgtaagaggt aaaggttaac aattctgata ccgcgcggca
    60541 gtatgctcct ctgccatcat tatcagaaaa attactgcca tcacagagtc tgccgccttc
    60601 catcaggtag gtcgaaagcg tggtggtcac tgttgacccc gtggactgaa ttcgtaacgc
    60661 cttattatgg ctgacataat tttcactcca ggagatatcg tatgaagcga ccggtatcgt
    60721 tgggcatatt gtgttatgca ctgtaggggc cgtatagctt ccaaatccgg gtaaatctgt
    60781 ataggaataa aaggatgcga tccaagggca accataatca tcagatgcaa cggtaatttt
    60841 aaacgtcata tatgcacaac gaaaaatttt cgtatctatg ccagggcaat ttgagttatt
    60901 aattgtatca aagtcagtca gtaccaaagt tggagctgaa cccgacccag accaggaaat
    60961 aacccgcaac gtatcgcctt tatacgcaaa agaatcatat ttcccatatg caacctcctt
    61021 tggcgttatc accgtaacat tgaaaacact gctcgctaca gcaccggtta ccttgtccat
    61081 ggcataataa actctcacac tgtcaaaact cgcactcata agtttatctc cagcataact
    61141 cttagatgcg ccgacaacac tcagaacgag cagcattagg ctcgcgaaaa ttatggtcca
    61201 ttttttcatt tcagaaatac tcaaaggttg ccgtcactgt ggcactaaag tctccggacg
    61261 ttacatctcg cccgctcttt gcctgtaacc aggtattaaa atttaggctg ttatttccct
    61321 gattcagcgg gagcgtgacg cctgttgtat tgttgattgg caccgatgtg cctgccgcag
    61381 tttcaatacc aatcccagcg ccggatgctg atgacgagga gtcaaaggcc agaaagccgg
    61441 gtagagcact atcttctgtt cctgtcaacg ttactttgac attaaacaat gtagaactat
    61501 gacaatcttt caattgaaac accaatggca ctcgctctga ctgcccaagg ttcattaaat
    61561 cactggcggc aatggcagga aaatgtactt ctgccaactg gctaccatta atcactggcg
    61621 tacacgattt actgattaaa ttgccggtaa agtgcaaatt atctcctgct attgtggttc
    61681 cactgcccag caacatcagg ctactaataa aaaacaacgt acgctttttc ttcattggta
    61741 atctacctgt aacgtggccc aggcctcaaa gtccgcctct ggcagaatga caccactctt
    61801 ttttactggc acagccgtta acaccggtaa atcggtttca ttcaccacaa gtggcgtatt
    61861 gatcgtaaaa ctctgccctg cctgtttgag ctgaatgcct aaaccagtga tattgctgct
    61921 tactgcagca ttatcaaaat cgcttggtgt gccactccag ctcaaggtca tttccattaa
    61981 tgggtcgcga accgccgtat cgcaggtaat ggaatacggc acggtttgct cataattgct
    62041 gccattaatg tcatcaataa gcacatcctg aaaagttatt tcaatggttt gagcgttgct
    62101 aatcgtgcag ttaggcggct gcactaaata accgtgaaaa gagacgtttg agagtgccat
    62161 cgagcaaaag ccccacagca aaataagcga gatccgtttc attggtaatc caccacaatt
    62221 gtggcactgg cgttaaattc agctgccgct aacgttgtac cactctgttt caccggcaca
    62281 gcttccagaa cgggaactcc cgaggagaaa ttaaatggca accatgagcc agaggttaag
    62341 tcgagtattg tgtgatcact acttttctga acccgaatac caaacccagg aatacccgtg
    62401 ctgataactg tttcaccgtt aatcacgacc gttgtcgcct gcatctgtag acgcaggtca
    62461 tccattgcca gattgttaca aacaagcgag tagtcaatgg gacgtttata atcaacccta
    62521 ttgatcttat taatgaatac attaccaaac tcaactgccg caccatttac cgtacaagga
    62581 ggaggggcat cgacaagcac ccgtaaattc atacttactg acttactcgc tgcaactgca
    62641 aagccgctac caacacaaca tatcagtaac ccagttaccg cccagacaga acaagatcct
    62701 ttcatactgg ctctcccggc ctcagccttt gcggctctgc tcttcatcga cctagcaact
    62761 gttaccgttg caagtgaaaa agagcggcat ccgagcgcca tagtcgttga cataggtcag
    62821 taccggcacg ctacccatct tcacattcag cggtgcagag gttttcggcg acatcactat
    62881 tggcgagaaa ccggctgccg gattgccatt tttctgtgtg ctggcattac tgaaaatgac
    62941 gtaatacgac gtcgggttat tgacggtaaa ttcctgaccg ttgcgggtta aagtaatctt
    63001 atattgctag gggtttttca tgctgacgtt ctccagcgcc ttcggtcgcc agaacaactt
    63061 aatacgcgtt tgtagtgcta tttgcagcgt gttggctttg tttgattttg gcgggatctc
    63121 acgcacgttg aagtagaaca ggctttcacg atcggcaggc aatttgttga tatccggcat
    63181 tccctgcact tttacctgcc cgttcatcat tgaatcgatg cgttgcaccg gtggcagaac
    63241 agttaacggt gaactgatct tgttaccctt ttcatcttcg atccagcttt gggccagata
    63301 aggcagtttc ggatcgttat tgcgcaacgt cacgctgatt gatttgtcgc tttcattaaa
    63361 tatcaaacgg gtacgatctg gcgttacaga agcctgggca gacaaactgg cagcgctaag
    63421 cagtaacgct agcgtcgtag cacccaattt ggcagagcaa agtaaatcag acatggatca
    63481 ttccttaatc acgttattgg ttagctgaaa cagaaacagg gggattggtg ggtgtcacct
    63541 gttgcgtctg ctcctgaatg acaggcttta tgtcatcagg aacaaccggc gcgacgttgc
    63601 ctttatgctg gcatggcagt aacaagccgt taaacagatc ggcaggcagc gggtccggca
    63661 ggttgatatc gcaatgcgca acaccactcc agaacacact catgtgttcg ccaggtttca
    63721 ctccagccag ataaacattg ccgtcatcat cgacaaggcc cactgtctgc tcgttatcat
    63781 tttttacttc tgcgccaaac ggaggatggc tgccgtcttg taaacgcagc accgccatcg
    63841 ctttttgacc actgatgacg gcaaatttgc ggtagccaat cgccccttca gttagcgtgg
    63901 cttgtaccac cgactgggtt gcttcggcgt tttccggcag tttgttgagg tcgatatacg
    63961 cctgattgcg gtaatagtta ttgacgtcag aaacgacggc tttaccaaac atattggtat
    64021 aaacagcagc accgttacct tctaccggaa catcggcaac gccatcggca tcaatcaaca
    64081 agcgtgtccc gcccatattc tgggtacggt gaagtgcgcc accgtgggca gtaagcgttg
    64141 cgccgccctg taacgacaag cccgcagagg tgtattgccc ttcatggtag ttcgcactga
    64201 ggtcaacctg cgccagcgaa ccatcatggc tgtaatagcc gtcaacgctg gtgtgtttgt
    64261 cactggtgcc gacgttcaac tgatagtgag tcgcgtcatc gacacggctg aaataaccga
    64321 cctgactgct gtccgtccca ctgccatagt accgttatag ctaacggtac tgttgtcgcc
    64381 ccacggcata ctgagcgaaa tgtacatgcc tttgtcggcc tggttgtcat actcgtaacg
    64441 gtagccagtc atcgagatgc tgacattacg aatactgccc atattgaaat agtgcgagag
    64501 catgatgttg tagtttgtct gctcctcgcg atcccagtag gtatggcggg tatagttgag
    64561 ataaaccgaa acacccgcat cgcggaagtt ctggttataa gtggcggtgt acatctcttt
    64621 gtcgttgccc gtgcgtacca ttccgctgtc actggcatcc agatactcgc tcatggtcat
    64681 aaagttctct tccgagaagc gatatccagc aaaagtaacg cggctgttga gctggtcaaa
    64741 gtctttggaa taactcacac ggaaggagtt accgtccagc gaacctttgc cataagcggt
    64801 gtctttatcc agtttggtgt gcgagtgagt aacatcaaac gcgaccgcgc cgaatgtaga
    64861 caaatcgcga ccgacaccaa gcgccgcaga ctgatagttt tcatctccca gtgcgccgcc
    64921 atataacgac cagccgttag cgatccccca ggaagcttca gcaccagaaa aaaattcacc
    64981 ctcgacatgg tgtccccact cttgcggacg gcccatcatg atcttatagc gaacctgacc
    65041 tggacgagtg aggtatggca tcgaggcggt gctgatgtca tattcctgca cctggccgtt
    65101 ctgttcttca atgcgaatat gcaacgtacc ggagacagaa tcaccaagat cctgaatacg
    65161 aaacggtcca gccggaacct gcgtttcgta aatcacacgc cccatctggc tgacggtcac
    65221 ttttgctgtg gtgtgtgcca cgccggaaat gtctggcgcg tagccgcgca gattgggagg
    65281 caacatttga tcgtcagtac tgacactgcc accaacatag ttaaaaccat caaaaatatc
    65341 ggatctgagg taatcctcgc ccagcgccag tttggctttt aatgatggta acgcccgcct
    65401 ggcatagtag cgactccact cccatttttt tgagtttcat cgccgctgaa ttcatcgtca
    65461 tcattactgc gagtatgttg atagttagtc tgccagtcag cacgcatacg ccacggccct
    65521 aggttaaccc caaccgtccc gttgccgctg atctcgttac tatcatcacc gccattttct
    65581 tcgtgccggg tttgtgcgtt gatgctgtag tccgcgacga tcccggagat gccgtcatcc
    65641 caacgtgaag gcggatccct gtcgggataa gtatattcga ggtaagcctg tggcagtgaa
    65701 atgactaatg cggactggct taaatcagcc ttaatttcca tgccttccag ttgaccggat
    65761 ttcaggcatt tagcatcgtg gctccattgc agatttttcg ccacgtcttc ttttaaacca
    65821 aactgcgcta ccagttccgg tgtcagacaa gcatagcttt tgctcgcgtc atcttcacca
    65881 gcataccagt aaatatcgta ctcttccgcc aatggctgtt tatttagttg aacctgtaaa
    65941 ttgtattttc cgggctcaac atatccctgg ctggaaaaac gcttcatatc aatttttgtg
    66001 tcgcctttta attccagaaa acgggaatca aactgaatgt cgtcttcagc ccagacactg
    66061 ctataactcc ctgacattgc cagcgcaatg caccacggaa gaatccgtaa tcgaaaaaga
    66121 gaatggtcag gcatacacgt ttctatccgt ttttattctt gttcggtcaa aatacattta
    66181 aatcccccat agcagtgtcg ctatgggggt atgccttaca ggtaagtaat ctggaaggtg
    66241 gtatttgctt caaaattacc cagatctggc gcatcagctg cgccgaccag ccaggcgtta
    66301 aagttcagtg tctgcttcgc tttgcctttg ttggtagaag tgtctttcac tattttctgg
    66361 tcaataccca tgccgctttt gtaagaggta cccagagagt caccgatcgc caggctaaca
    66421 ttgttaaatg ccgcaccggt atcggtattg aaaatggtgt aataattgcc gctatttgca
    66481 gaagaaacgg taccagtaaa ggtagtggtc attgtttcct gagtatcaaa tacgcaatct
    66541 tgcagacgaa tctggaaact tttcggcgtg gtggtgccgt tatttttcag tttttcggca
    66601 ccgatatcac ccatatcaac ttccagtttg tgatcgtccg gaacgataga acacactgag
    66661 tcttcaatgg tgccgtaaaa acgtgcagta ccgttgttac ccgcagcgat tgttgccgta
    66721 gaggtgaaca cgcccatcac cattgccgca gcaatagctg ttttaacaaa cttactcatt
    66781 atttgtcctt aaactataaa taattaaaaa tataaacatt gcaatacatt gattcagtca
    66841 atagccaatg tttataccca gccattgaat acggcatgcg acccaaaaag aacaggtcgt
    66901 agaaaattgt attcattggt gtggggccgc atagtgatgt ttccacaccc ctgatccaat
    66961 ttaattttac taaagagtaa atgtagattt aattaatata ttgacagaag aaaggttatc
    67021 ttaagga
//
LOCUS       DAZOYF010000005        65533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZOYF010000005 DAZOYF010000000
VERSION     DAZOYF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.005"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..452)
                     /gene="folK"
                     /locus_tag="ACHUOF_000312"
     CDS             complement(<1..452)
                     /gene="folK"
                     /locus_tag="ACHUOF_000312"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR0609656.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHT"
     gene            complement(449..1846)
                     /gene="pcnB"
                     /locus_tag="ACHUOF_000313"
     CDS             complement(449..1846)
                     /gene="pcnB"
                     /locus_tag="ACHUOF_000313"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR0609657.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(1906..2802)
                     /gene="gluQRS"
                     /locus_tag="ACHUOF_000314"
     CDS             complement(1906..2802)
                     /gene="gluQRS"
                     /locus_tag="ACHUOF_000314"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR0609658.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(2869..3324)
                     /gene="dksA"
                     /locus_tag="ACHUOF_000315"
     CDS             complement(2869..3324)
                     /gene="dksA"
                     /locus_tag="ACHUOF_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR0609659.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3502..4206)
                     /gene="sfsA"
                     /locus_tag="ACHUOF_000316"
     CDS             complement(3502..4206)
                     /gene="sfsA"
                     /locus_tag="ACHUOF_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR0609660.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4221..4751)
                     /gene="thpR"
                     /locus_tag="ACHUOF_000317"
     CDS             complement(4221..4751)
                     /gene="thpR"
                     /locus_tag="ACHUOF_000317"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR0609661.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4825..7254
                     /gene="hrpB"
                     /locus_tag="ACHUOF_000318"
     CDS             4825..7254
                     /gene="hrpB"
                     /locus_tag="ACHUOF_000318"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR0609662.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPKILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7450..9984
                     /gene="mrcB"
                     /locus_tag="ACHUOF_000319"
     CDS             7450..9984
                     /gene="mrcB"
                     /locus_tag="ACHUOF_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR0609663.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10204..12447
                     /gene="fhuA"
                     /locus_tag="ACHUOF_000320"
     CDS             10204..12447
                     /gene="fhuA"
                     /locus_tag="ACHUOF_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR0609664.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12498..13295
                     /gene="fhuC"
                     /locus_tag="ACHUOF_000321"
     CDS             12498..13295
                     /gene="fhuC"
                     /locus_tag="ACHUOF_000321"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR0609665.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13295..14185
                     /gene="fhuD"
                     /locus_tag="ACHUOF_000322"
     CDS             13295..14185
                     /gene="fhuD"
                     /locus_tag="ACHUOF_000322"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR0609666.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14182..16164
                     /gene="fhuB"
                     /locus_tag="ACHUOF_000323"
     CDS             14182..16164
                     /gene="fhuB"
                     /locus_tag="ACHUOF_000323"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR0609667.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16199..17479)
                     /gene="hemL"
                     /locus_tag="ACHUOF_000324"
     CDS             complement(16199..17479)
                     /gene="hemL"
                     /locus_tag="ACHUOF_000324"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR0609668.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17645..17692
                     /gene="yadX"
                     /locus_tag="ACHUOF_000325"
     CDS             17645..17692
                     /gene="yadX"
                     /locus_tag="ACHUOF_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR0609669.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17704..19125
                     /gene="clcA"
                     /locus_tag="ACHUOF_000326"
     CDS             17704..19125
                     /gene="clcA"
                     /locus_tag="ACHUOF_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR0609670.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19149..19214
                     /gene="yadW"
                     /locus_tag="ACHUOF_000327"
     CDS             19149..19214
                     /gene="yadW"
                     /locus_tag="ACHUOF_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR0609671.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19207..19551
                     /gene="erpA"
                     /locus_tag="ACHUOF_000328"
     CDS             19207..19551
                     /gene="erpA"
                     /locus_tag="ACHUOF_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR0609672.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19598..20221)
                     /locus_tag="ACHUOF_000329"
     CDS             complement(19598..20221)
                     /locus_tag="ACHUOF_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR0609673.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20259..21059)
                     /gene="btuF"
                     /locus_tag="ACHUOF_000330"
     CDS             complement(20259..21059)
                     /gene="btuF"
                     /locus_tag="ACHUOF_000330"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR0609674.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21052..21750)
                     /gene="mtnN"
                     /locus_tag="ACHUOF_000331"
     CDS             complement(21052..21750)
                     /gene="mtnN"
                     /locus_tag="ACHUOF_000331"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR0609675.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21834..23351
                     /gene="dgt"
                     /locus_tag="ACHUOF_000332"
     CDS             21834..23351
                     /gene="dgt"
                     /locus_tag="ACHUOF_000332"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR0609676.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23481..24905
                     /gene="degP"
                     /locus_tag="ACHUOF_000333"
     CDS             23481..24905
                     /gene="degP"
                     /locus_tag="ACHUOF_000333"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR0609677.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25060..26216
                     /gene="cdaR"
                     /locus_tag="ACHUOF_000334"
                     /pseudo
     CDS             25060..26216
                     /gene="cdaR"
                     /locus_tag="ACHUOF_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26305..26691)
                     /locus_tag="ACHUOF_000335"
     CDS             complement(26305..26691)
                     /locus_tag="ACHUOF_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR0609678.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27005..27829)
                     /gene="dapD"
                     /locus_tag="ACHUOF_000336"
     CDS             complement(27005..27829)
                     /gene="dapD"
                     /locus_tag="ACHUOF_000336"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR0609679.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27860..30532)
                     /gene="glnD"
                     /locus_tag="ACHUOF_000337"
     CDS             complement(27860..30532)
                     /gene="glnD"
                     /locus_tag="ACHUOF_000337"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR0609680.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30594..31388)
                     /gene="map"
                     /locus_tag="ACHUOF_000338"
     CDS             complement(30594..31388)
                     /gene="map"
                     /locus_tag="ACHUOF_000338"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR0609681.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31756..32481
                     /gene="rpsB"
                     /locus_tag="ACHUOF_000339"
     CDS             31756..32481
                     /gene="rpsB"
                     /locus_tag="ACHUOF_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR0609682.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32739..33590
                     /gene="tsf"
                     /locus_tag="ACHUOF_000340"
     CDS             32739..33590
                     /gene="tsf"
                     /locus_tag="ACHUOF_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR0609683.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33737..34462
                     /gene="pyrH"
                     /locus_tag="ACHUOF_000341"
     CDS             33737..34462
                     /gene="pyrH"
                     /locus_tag="ACHUOF_000341"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR0609684.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34754..35311
                     /gene="frr"
                     /locus_tag="ACHUOF_000342"
     CDS             34754..35311
                     /gene="frr"
                     /locus_tag="ACHUOF_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR0609685.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35403..36599
                     /gene="ispC"
                     /locus_tag="ACHUOF_000343"
     CDS             35403..36599
                     /gene="ispC"
                     /locus_tag="ACHUOF_000343"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR0609686.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36788..37546
                     /gene="ispU"
                     /locus_tag="ACHUOF_000344"
                     /gene_synonym="uppS"
     CDS             36788..37546
                     /gene="ispU"
                     /locus_tag="ACHUOF_000344"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR0609687.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37559..38416
                     /gene="cdsA"
                     /locus_tag="ACHUOF_000345"
     CDS             37559..38416
                     /gene="cdsA"
                     /locus_tag="ACHUOF_000345"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR0609688.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38428..39780
                     /gene="rseP"
                     /locus_tag="ACHUOF_000346"
     CDS             38428..39780
                     /gene="rseP"
                     /locus_tag="ACHUOF_000346"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR0609689.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39810..42242
                     /gene="bamA"
                     /locus_tag="ACHUOF_000347"
     CDS             39810..42242
                     /gene="bamA"
                     /locus_tag="ACHUOF_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR0609690.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42364..42849
                     /gene="skp"
                     /locus_tag="ACHUOF_000348"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42364..42849
                     /gene="skp"
                     /locus_tag="ACHUOF_000348"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR0609691.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42853..43878
                     /gene="lpxD"
                     /locus_tag="ACHUOF_000349"
     CDS             42853..43878
                     /gene="lpxD"
                     /locus_tag="ACHUOF_000349"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR0609692.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43983..44438
                     /gene="fabZ"
                     /locus_tag="ACHUOF_000350"
     CDS             43983..44438
                     /gene="fabZ"
                     /locus_tag="ACHUOF_000350"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR0609693.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44442..45230
                     /gene="lpxA"
                     /locus_tag="ACHUOF_000351"
     CDS             44442..45230
                     /gene="lpxA"
                     /locus_tag="ACHUOF_000351"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR0609694.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45230..46378
                     /gene="lpxB"
                     /locus_tag="ACHUOF_000352"
     CDS             45230..46378
                     /gene="lpxB"
                     /locus_tag="ACHUOF_000352"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR0609695.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46375..46971
                     /gene="rnhB"
                     /locus_tag="ACHUOF_000353"
     CDS             46375..46971
                     /gene="rnhB"
                     /locus_tag="ACHUOF_000353"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR0609696.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47008..50490
                     /gene="dnaE"
                     /locus_tag="ACHUOF_000354"
     CDS             47008..50490
                     /gene="dnaE"
                     /locus_tag="ACHUOF_000354"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR0609697.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50503..51462
                     /gene="accA"
                     /locus_tag="ACHUOF_000355"
     CDS             50503..51462
                     /gene="accA"
                     /locus_tag="ACHUOF_000355"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR0609698.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51561..53702
                     /gene="ldcC"
                     /locus_tag="ACHUOF_000356"
     CDS             51561..53702
                     /gene="ldcC"
                     /locus_tag="ACHUOF_000356"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR0609699.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53759..54148
                     /locus_tag="ACHUOF_000357"
     CDS             53759..54148
                     /locus_tag="ACHUOF_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0609700.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54213..55511
                     /gene="tilS"
                     /locus_tag="ACHUOF_000358"
     CDS             54213..55511
                     /gene="tilS"
                     /locus_tag="ACHUOF_000358"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR0609701.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55560..55820)
                     /gene="rof"
                     /locus_tag="ACHUOF_000359"
     CDS             complement(55560..55820)
                     /gene="rof"
                     /locus_tag="ACHUOF_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR0609702.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55807..56007)
                     /locus_tag="ACHUOF_000360"
     CDS             complement(55807..56007)
                     /locus_tag="ACHUOF_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR0609703.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56173..56718
                     /locus_tag="ACHUOF_000361"
     CDS             56173..56718
                     /locus_tag="ACHUOF_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR0609704.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56715..57137
                     /gene="arfB"
                     /locus_tag="ACHUOF_000362"
     CDS             56715..57137
                     /gene="arfB"
                     /locus_tag="ACHUOF_000362"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFR0609705.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57151..57861
                     /gene="nlpE"
                     /locus_tag="ACHUOF_000363"
                     /gene_synonym="cutF"
     CDS             57151..57861
                     /gene="nlpE"
                     /locus_tag="ACHUOF_000363"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR0609706.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58016..58840)
                     /locus_tag="ACHUOF_000364"
     CDS             complement(58016..58840)
                     /locus_tag="ACHUOF_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR0609707.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58893..60611)
                     /gene="proS"
                     /locus_tag="ACHUOF_000365"
     CDS             complement(58893..60611)
                     /gene="proS"
                     /locus_tag="ACHUOF_000365"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR0609708.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60722..61429)
                     /gene="tsaA"
                     /locus_tag="ACHUOF_000366"
     CDS             complement(60722..61429)
                     /gene="tsaA"
                     /locus_tag="ACHUOF_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR0609709.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61426..61830)
                     /gene="rcsF"
                     /locus_tag="ACHUOF_000367"
     CDS             complement(61426..61830)
                     /gene="rcsF"
                     /locus_tag="ACHUOF_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR0609710.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61948..62763)
                     /gene="metQ"
                     /locus_tag="ACHUOF_000368"
     CDS             complement(61948..62763)
                     /gene="metQ"
                     /locus_tag="ACHUOF_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR0609711.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62803..63456)
                     /locus_tag="ACHUOF_000369"
     CDS             complement(62803..63456)
                     /locus_tag="ACHUOF_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR0609712.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63449..64480)
                     /gene="metN"
                     /locus_tag="ACHUOF_000370"
     CDS             complement(63449..64480)
                     /gene="metN"
                     /locus_tag="ACHUOF_000370"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR0609713.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64668..65243
                     /gene="gmhB"
                     /locus_tag="ACHUOF_000371"
     CDS             64668..65243
                     /gene="gmhB"
                     /locus_tag="ACHUOF_000371"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR0609714.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15476 a        16265 c        18073 g        15719 t
ORIGIN      
        1 cttgtatgta agacttcacg caacgtctcc ccatcaggaa acgccagctc cggtgcaatc
       61 tcaaacagcg gccacagcat aaatccacga ttcttcatat cgtagtgcgg aacggtcagg
      121 cgttcggtat ttatcacttc attaccaaac agcatgatat cgagatccag cgtgcgtggt
      181 ccccagcgtt cagctttacg aacacgaccc tgttgcagtt ctatgcgctg tgtatggttg
      241 agtaactctt ccggggcggg cgaagtttca agcgccacgg cggcgttgag gtaatcgggt
      301 tgatcttgcg gccccagcgg tggggtgcgg taaaacgaag aaacggcaag aatgcggctt
      361 tcagggatat cgcctaatgc tttcagggca gcattgacct gctccagcgg agaggccaga
      421 ttgctgccta tggcaatata cgccactgtc atgtggtacc ctcacgacgc ggtgcgcgtt
      481 tgcgtggacg acgagtacga cgacgcggtg acggttcttc atccagctcg ttaagcatcc
      541 ctttttgatc tggtggtgcg gaaacctgga actcacccca ccatttcacc agacgctgca
      601 gttcagcatt acgttcaact tcagctcgca aggccaacag gtcataagcc gcacggaact
      661 taggatgctc cagcagtttc catgcgcgtt taccctgacg acgggacata cgcaactgca
      721 actgccagat atcgcgggtt aacgtcgtca gacgtttcgg gattgccagt gaacggcagg
      781 cttcgtccag cacgtcgttc atcgccagcg cgaaagcgtc gtgataggtc aggccgcttt
      841 cctgggcgat cttctgtgcc gtctccagca gtgggtacca gaacatggcg gcaaacaaga
      901 acgccgggtt cacgcgcata tcgttatgga tacgcgtatc ggtattcttc agcacctgtt
      961 caatgatccg ctccatcggg ctgtcgccat tttccgtgaa gtagcgggta atggtcggga
     1021 acagcggctg gaacagatga tattcacaca acagcttata ggtttcgtaa ccgtagcccg
     1081 cttgtagcag tttaagcgat tcttcaaaca ggcgtgccgg tgggatatcg ttcagcaggg
     1141 tagcgaggcg agggatcggt tctgcggttt ccgggctgat gcgcataccc aatttggcgg
     1201 caaaacgtac cgcgcgcagc atacgtaccg gatcttcacg gtagcgcgtt tccgggttac
     1261 caatcagacg gataacgccg tccttcagat ccttcatacc accaacgtaa tcacggacgg
     1321 taaaatccgc tacgctgtaa tacaggctgt tgatagtgaa atcgcggcgc tgggcgtctt
     1381 cttcgatgga gccgaaaatg ttgtcgcgca gcaacatgcc gttttgcccg cgttgggagg
     1441 tcgtgcggtc gctgacgtta ccttcgtggt gtccacggaa ggtcgcaact tcgataatct
     1501 ccgggccaaa cattacatga gccagacgga aacggcgacc caccaggcgg cagttacgga
     1561 acagtttgcg cacctgctca ggcgtggcgt tagtggttac gtcaaaatct ttcggctttt
     1621 tgccaagtaa caggtcgcgc acgccgccgc caaccagcca ggcttcgtat cccgctttgt
     1681 tgagcctgta cattaccttc agggcatttt cactgatatc tttgcgggaa atagcatgct
     1741 gctcacgcgg gatcaccgtc acctgtggac gggcgactgc ctgttcagcc tcgctttcct
     1801 cgcggcttag caccttgcgg caaaaattag cgactcgggt aaaaatagta cacctcggta
     1861 gtgtcaaaca tcattcagga caaaaaaata gcggctaatc atagctcagc atgacgcatt
     1921 tgagaatgtt gaatttacaa ttgccgactc gggcacggcg gtcagcatcc agtttttgac
     1981 ggctgactga aggatttgct cgacgctgaa atcctgccag tgtgcttctg cctgctgccc
     2041 cagaaattga agtgccgcga ttagtaccgg gcgtggatcg cctttcggca acgcaggcgc
     2101 atgattctgc ttggaaagtt tagcgccttg tggattaagc gccagcggca gatgaatgta
     2161 atctggcact ttccagccaa aaagctggta cagcgggatt tgccttactg ttggttcaat
     2221 cagatcagcc ccacgcacta tttctgtaac gccctggaaa tgatcatcaa ccacaacagc
     2281 caggttgtag gcgaacaacc catcacggcg atgaatgata aaatcttccc gtgccagttt
     2341 ttcgtcggcg tgaataatgc ctcgcagcag gtcagtaaat tgcgtgaccg gatgctgctg
     2401 gcggatacgc actgcggcgt tgtctggtcc atgatgcaac acccggcaat gaccgtcgta
     2461 aataccgcca atgctttgaa tacgcgcacg cgtacaggtg cagtaataac ttagtccttg
     2521 ttcatgtaac caggcgagtg cttcacgata ggcgtcgtga cgttgcgatt gccagagaac
     2581 atcgccgtcc cagtgcagac cgtaatgttc cagctggcgc aggatagttt ctgcggcacc
     2641 gggaacttca cgaggcgggt cgatatcttc tatgcgtacc agccagcgac cttgccgggc
     2701 gcgagcctgc aaatagctgc cgagcgcggc gatcagagag ccaaaatgaa gctcgccgga
     2761 aggagagggg gcgaagcggc caatatactg tgtgtctgtc atctctttga acaaaaaata
     2821 aggcgggagc atttcccgcc tgtggtaaac gtgatggaac ggctgtaatt agccagccat
     2881 ctgtttttcg cgaatttcag ccagcgtttt gcagtcgatg cacagatcgg ctgtcgggcg
     2941 cgcttccaga cggcgaatac caatttcaac accgcaggat tcgcagtagc cgaaatcttc
     3001 gtcttccact tttttcagcg tcttctcgat ctttttgatc agcttacgct cgcgatcgcg
     3061 gttacgcagt tcgaggctga actcttcttc ctgggctgca cggtctaccg ggtccgggaa
     3121 gttggctgct tcatcctgca tatgtgtaac ggtgcgatcg acttcatccc tgagttgatt
     3181 acgccatgct tccagaatac gacggaagtg cgccagctgg gcttcattca tatactcttc
     3241 gcccggcttc tcctgatatg gttccacccc agcgatggcg agaatactca gggacgatgt
     3301 tttacggttt tgcccttctt gcatgttgct tctccttaac acgcactatc gatccccatg
     3361 ttcgggggaa aaatgaggcc gctataaata gcagatgctt ttccggatag caattatcta
     3421 aacgtaacac ttgacaactg tgtgaggaaa agcgtatttg cgcacgcgac cagaatgtaa
     3481 attaaccagt tacttacttt actacaatgt aaccggcagt gattttttaa gagccatgcc
     3541 ttcagcagaa atttccgctt tgtaagccag aatttctacc cccctctgtt gagcttctga
     3601 caatagttgc gcgtatttct catcgatgtg gcgcgcgggt gaaaaccgtg taatggctga
     3661 atgcagcacg gcgaaaaaaa taaccgcacg ctggccttca gccgctacgc tcatcaactc
     3721 ccgaaggtgt ttctgacctc gttcagtgac cgcatcggga aaatatccct gttcgttctc
     3781 cgctaacgta accgatttca cttcaatata gcagtctgga cgcgaatccg cctgcaacat
     3841 aaagtcaata cggctgcgtt cggagccgta ttttacttcg cttttcagcg agctatagcc
     3901 tgacagttct gaaattgatt cattaaggat agcctctttc gtcaacctgt tagcccaaag
     3961 cgtattgacg caaataaatg cgccgctctg gctttgagtt aattcccagg tgtgtgggta
     4021 tttccgtttg gtgttgtctg aagtcgaata ccagacggta tcgccaggcg ttgcacaacc
     4081 ggtcatcgca cccgtattcg ggcagtgtag cgttaattcg cgaccatcgg gtgtgatcac
     4141 atcggctaaa aaacgtttgt aacgctgaat tagcgtcgcg cgctgtagag ggggagaaaa
     4201 ttccattgcg acaatccttg ttattgcgtt agcgcccagc gttttagcgg cgtgtagcgt
     4261 gtgcgtccac gggcaaacga ggaggcgtaa agggtgaact ccgtcaccgc ataagaccag
     4321 ttaaaacccg gcggcgggaa tgtcaccgcc tcgctggcgt cgcgcaataa ggtaatatgt
     4381 ggatgaaacg gacgattgct ttgaaaacaa ccgctgcggg cagcctgtga acggagcata
     4441 ttcgccagct ggattaagcc gcgtggaggc tgacgcatcc ctaaccacac cacacgcgaa
     4501 cgcagccatt gtccggcgtc atcaagcgtg agtgtgaaac caggttgacg aatccgtccg
     4561 gctaaaagag aaagcgcctt ctctttctct gcgctcactt cgcctaaaaa tgccagagtc
     4621 aggtgcaaat tatcggcggc gaccggacgt cccgcctcag gtgggaagtg tgtggcgcgc
     4681 caatggataa tctgttcgcg gatttctgca ggtaagtcga tagcaaagaa cagacgttgc
     4741 ggttcagaca tgtgaggcac tcggttatga attaccgcga tgctacaatg tggcgcgaag
     4801 aatgttaacc ctctggagcg ttttgtgtcg tcgttgcccg ttgctgccgt cttacctgaa
     4861 ttacttaccg ctctcgattg tgcgccgcag gtattattaa gtgcgccgac cggggccggg
     4921 aaatcaacct ggctgccgct gcaactgctg gcgcatcccg gcattaacgg gaaaattatc
     4981 ctgctggagc cgcgtcgtct ggcggcgcgt aacgtcgcgc aacggctggc ggagctgctt
     5041 aacgaaaagc caggcgatac cgttggctac cggatgcgtg cgcaaaactg cgtcgggctg
     5101 aatacccgcc tggaagtggt taccgaaggc gtgctgacgc ggatgatcca gcgtgacccg
     5161 gaactgagcg gtgttggatt ggtgatcctt gatgagtttc atgagcgcag tttgcaggcg
     5221 gatttggcgt tggcgctgtt actcgatgtg caacaaggtc tgcgtgatga ccttaaactg
     5281 ctgattatgt cggctacgct ggacaatgac cgcttgcagc aaatgctgcc ggaagcgccc
     5341 gtcgtcatct cagaagggcg ctcgtttccg gttgaacgcc gttatttacc gctgcccgcg
     5401 catcagcgtt ttgacgaagc cgttgcggta gccaccgccg aaatggtgcg tcaggaaagt
     5461 ggatcattac tgttattttt acctggcgtc ggagaaattc agcgcgtgca ggaacaactg
     5521 gcttcgcgca tcggcagtga tgtggtgctc tgcccgctgt atggcgtgtt gtcactgaac
     5581 gatcagcgaa aagcgatcct cccggcaccg caagggatgc gcaaagtggt gctggcgacc
     5641 aatattgctg aaaccagttt aaccatcgaa ggtattcgtc tggtggtgga ttgtgcccag
     5701 gagcgtgtgg cgcgctttga tccgcgcacg gggcttacgc ggttgattac tcaacgcgtt
     5761 agtcaggcat ccatgacgca gcgtgccggg cgcgccgggc gtctggagcc gggtatcagc
     5821 ctgcatttaa tcgccaaaga acaagcagaa cgcgccgcgg cgcaaagtga gccgaagatc
     5881 ttacaaagcg atctttccgg tttgctgatg gaattactgc aatggggatg cagcgatccg
     5941 gcgcagatga gctggctgga tcaaccgcca acggtgaatc tactggccgc gaaacgcctg
     6001 ttacaaatgc tgggggcgct ggagggtgaa cggcttagtg cgcaagggca aaaaatggca
     6061 gcgctgggta acgatccgcg tttagcggca atgctggtta acgcaaagag cgatgacgaa
     6121 gctgctaccg cggcaaaaat tgccgccatt ctcgaagagc cgccacggat gggcaatagt
     6181 gacctgggcg tggcgttttc gcgcaatcaa ccagcctggc agcaacgtag tcagcaactg
     6241 ttaaaacgct taaacgtacg cggcggtgag gcagacagtt cgcttatcgc gccgctactt
     6301 gccggggcgt ttgccgatcg cattgctcat cgccgtgggc aagatggacg ctatcaactg
     6361 gcaaacagca tgggagcgat gctcgatgcc gacgacgcgc taagccgcca cgaatggttg
     6421 atcgcaccgt tattattgca gggcagcgcc tcgccggatg cgcggatttt actggcgcta
     6481 ccggtcgata ttgatgaatt agtacaacgc tgtccgcagc tggtgcagca atctgacacc
     6541 gtggaatggg atgacgcgca aggtacgctg aaagcctggc gtcggctaca aatcggtcag
     6601 ttgacggtga aagtgcagcc gctggcgaaa ccgtcagaag acgagttgca tcaggcgatg
     6661 cttaatggca tccgtgataa aggtttaagc gtgctcaact ggacggcgga agcggaacag
     6721 ctacgcttgc gtttgttatg cgccgcaaag tggttgccgg aatatgactg gccagcggtt
     6781 gatgatgaaa gtttgttggc gacgctggaa acgtggctgc tgccacatat gaccggcgta
     6841 cattcactac gcggcctgaa atcactcgac atttatcagg cactacgcgg attacttgat
     6901 tggggaatgc agcaacgtct ggatagtgaa ttgcctgcgc attacactgt gccgacggga
     6961 agccggatcg ccattcgtta tcatgaagat aacccgcccg cgctggcggt gagaatgcag
     7021 gagatgtttg gtgaggccac taatccgacg atcgcccagg ggcgcgtgcc gctggtgctg
     7081 aagttgcttt cccctgccca aaggccgctg caaatcacgc gtgatttggg ggccttctgg
     7141 aaaggggcgt atcgtgaggt gcaaaaggag atgaaagggc gttatcccaa acacgtctgg
     7201 ccggacgacc cggcaaacac ggcaccgacg cgacggacga aaaagtattc gtaaggttgg
     7261 ttttctccct ctccctgtgg gagagggccg gggtgagggc atcagcgcgc acgttcaccc
     7321 tcaccctaac cctctccctc aagggagagg ggaccgatcg agcgcaaatt ttgagagata
     7381 tcttcttctg tcttgtaaca gaagaacaga aaatcgggct tttgcgcctg aatattgcgg
     7441 agaaaaagca tggccgggaa tgaccgcgag ccaattggac gcaaagggaa accgacgcgt
     7501 ccggtcaaac aaaaggtaag ccgtcgtcgt tacgaagatg acgatgatta cgacgattat
     7561 gatgactatg aggatgaaga accgatgccg cgcaaaggta agggcaaagg caaagggcgt
     7621 aagcctcgtg gcaaacgcgg ctggctatgg ctactgctaa aactggctat cgtttttgcc
     7681 gtgctgatcg ccatttacgg cgtttatctc gatcaaaaaa ttcgtagccg tattgatggc
     7741 aaggtctggc aactgcccgc ggcagtttat ggccgaatgg tcaaccttga accagatatg
     7801 accatcagca agaacgaaat ggtgaagctg ctggaggcaa cacagtatcg tcaggtgtcg
     7861 aaaatgaccc gtcctggcga atttaccgtg caggccaaca gcattgagat gatccgccgt
     7921 ccgtttgatt tcccggacag taaagaaggg caggtgcgtg cgcgtctgac ctttgatggt
     7981 gatcatctgg cgaccatcgt caatatggag aacaaccgtc agttcggttt cttccgtctt
     8041 gatccgcgtc tgatcaccat gatctcctcg ccaaacggtg agcagcgttt gtttgtgccg
     8101 cgcagtggtt tccctgattt gctggtggat actttgctgg cgacagaaga ccgtcatttt
     8161 tacgagcatg atggaatcag tctctactcc attgggcgtg cggtgctggc aaacctgacc
     8221 gcaggtcgca cggtgcaggg tgcgagtacg ctgacgcaac agctggtgaa aaacctgttc
     8281 ctctccagcg agcgttctta ctggcgtaaa gcgaacgaag cttacatggc gctgatcatg
     8341 gacgcgcgtt acagcaaaga ccgtattctt gagctgtata tgaacgaggt gtatctcggt
     8401 cagagcggcg acaacgaaat ccgcggcttc ccgctggcaa gcttgtatta ctttggtcgc
     8461 ccggtagaag agctaagcct cgaccagcag gcgctgttag tcggtatggt gaaaggggcg
     8521 tccatctaca acccgtggcg taacccgaaa ctggcgctgg agcgacgtaa tctggtgctg
     8581 cgtctgctgc aacagcaaca gattattgat caagaactct atgacatgtt gagtgcccgt
     8641 ccgctggggg ttcagccgcg tggtggggtg atctctcctc agccagcctt tatgcagctg
     8701 gtgcgtcagg agttgcaggc aaaactgggc gataaggtaa aagatctctc cggcgtgaag
     8761 atcttcacta cctttgactc ggtggcccag gacgcggcag aaaaagccgc cgtggaaggc
     8821 attccggcac tgaagaaaca gcgtaagttg agcgatcttg aaactgcgat tgtggtcgtc
     8881 gaccgcttta gtggtgaagt tcgtgcgatg gtcggaggtt ctgagccgca gtttgcgggc
     8941 tacaaccgtg cgatgcaggc gcgtcgttcg attggttccc ttgcaaaacc agcgacttat
     9001 ctgacggctt taagccagcc gaaaatctat cgcctgaaca cgtggattgc cgatgcgcca
     9061 atcgcgctgc gtcagccgaa tggccaggtc tggtcaccgc agaacgatga ccgtcgttat
     9121 agcgaaagcg gcagagtgat gctggtggat gcgttgacac gttcgatgaa cgtgccgacg
     9181 gtaaatctgg ggatggcgct ggggctgcct gcggttacgg agacctggat caaactgggc
     9241 gtaccgaaag atcagttgca tccggttccg gcaatgctgc tgggggcgtt gaacttaacg
     9301 ccaatcgaag tggcgcaggc attccagacc atcgccagcg gtggtaaccg tgcaccgctt
     9361 tctgcactgc gttcggtaat cgcggaagat ggtaaggtgc tgtatcagag cttcccgcag
     9421 gcagaacgcg ctgttccggc gcaggcggcg tatctgacac tatggaccat gcagcaggtg
     9481 gtacaacgtg gtacgggtcg tcagcttggg gcgaaatacc cgaacctgca tctggcaggg
     9541 aaaacaggga ctaccaacaa taacgtagat acctggtttg caggcattga cggcagcacg
     9601 gtgaccatca cctgggtagg ccgtgataac aaccagccga ccaaactgta tggagccagc
     9661 ggggcaatgt cgatttatca gcgttatctg gctaaccaga cgccaacgcc gctgaatctt
     9721 gttccgccag aagatattgc agatatgggc gtggactacg acggcaactt tgtttgcagc
     9781 ggtggcatgc gtgtcttgcc ggtctggacc agcgatccgc aatcgctgtg ccagcagagc
     9841 gagatgcagc agcagccgtc aggcaatccg tttgatcagt cttctcagcc gcagcaacag
     9901 ccgcaacagc aacctgctca gcaagagcag aaagacagcg acggtgtagc cggttggatc
     9961 aaggatatgt ttggtagtaa ttaacatcta agcgtgaaat accggatggc gagttgccat
    10021 ccggtaaaat aacatcccat ctaagatatt aaccctttct tttcatctgg ttgtttatta
    10081 acccttcagg aacgctcaga ttgcgtaccg cttgcgaacc cgccagcgtt tcgaatatta
    10141 tcttatcttt ataataatca ttctcgttta cgttattatt cactttacat cagagatata
    10201 ccaatggcgc gttccaaaac tgctcagcca aaacactcac tgcgtaaaat cgcagttgta
    10261 gtagccacag cggttagcgg catgtctgtt tatgcacagg cagcggttga accgaaagaa
    10321 gacactatca ccgttaccgc tgcacctgcg ccgcaagaaa gcgcatgggg gcctgctgca
    10381 actattgcgg cgcggcagtc agctaccggc actaaaaccg atacgccgat tcaaaaagtg
    10441 ccacagtcta tttctgttgt gaccgccgaa gagatggcgc tgcatcagcc gaagtcggta
    10501 aaagaagcgc ttagctacac gccgggtgtc tctgttggta cgcgtggcgc atccaacacc
    10561 tatgaccacc tgatcattcg cggttttgcg gcagaaggcc aaagccaaaa taactatctg
    10621 aatggcctga agttgcaggg caacttctat aacgatgcgg tcattgaccc gtatatgctg
    10681 gaacgcgctg aaattatgcg tggcccggtt tccgtgcttt acggtaaaag cagtcctggc
    10741 ggtctgttga atatggtcag caagcgtccg accaccgaac cgctgaaaga agttcagttt
    10801 aaagccggta ctgacagcct gttccagact ggttttgact ttagcgatgc gttggatgat
    10861 gacggtgttt actcttatcg cctgaccggt cttgcgcgtt ctgccaatgc ccagcagaaa
    10921 gggtcagaag agcagcgtta tgctattgca ccggcgttca cctggcgtcc ggatgataaa
    10981 accaatttca ccttcctttc ttacttccag aacgagccgg aaaccggtta ttacggctgg
    11041 ttgccgaaag agggaaccgt tgagccgctg ccgaacggta agcgtctgcc gacagacttt
    11101 aatgaagggg cgaagaacaa cacctattct cgtaatgaga agatggtcgg ctacagcttc
    11161 gatcacgaat ttaacgacac ctttactgtg cgtcagaacc tgcgctttgc tgaaaacaaa
    11221 acctcgcaaa acagcgttta tggttacggc gtctgctccg atccggcgaa tgcttacagc
    11281 aaacagtgtg cggcattagc gccagcggat aaaggccatt atctggcacg taaatacgtc
    11341 gttgatgatg agaagctgca aaacttctcc gttgataccc agttgcagag caagtttgcc
    11401 actggcgata tcgaccacac cctgctgacc ggtgtcgact ttatgcgtat gcgtaatgac
    11461 atcaacgcct ggtttggtta cgacgactct gtgccactgt tcaatctgta caatccggtg
    11521 aatcccgatt tcgacttcaa tgccaaagat ccggcaaact ccggccctta ccgcattctg
    11581 aataaacaga aacaaacggg cgtttatgtt caggatcagg cgcagtggga taaagtgctg
    11641 gtcaccctag gcggtcgtta tgactgggca gatcaagaat ctcttaaccg cgttgctggg
    11701 acgaccgata aacgtgatga caaacagttt acctggcgtg gtggtgttaa ctacctgttt
    11761 gataatggtg taacacctta cttcagctat agcgaatcgt ttgaaccttc ttcgcaagtt
    11821 gggaaggatg gtaatatttt cgcaccgtct aaaggtaagc agtatgaagt cggcgtgaaa
    11881 tatgtaccgg aagatcgtcc gattgtagtt actggtgccg tgtataatct cactaaaacc
    11941 aacaacctga tggcggaccc tgagggttcc ttcttctcgg ttgaaggtgg cgagatccgc
    12001 gcacgtggcg tagaaatcga agcgaaagcg gcgctgtcgg cgagtgttaa cgtagtcggt
    12061 tcttatactt acaccgatgc ggaatacacc accgatacta cctataaagg caatacgcct
    12121 gcacaggtgc caaaacacat ggcttcgctg tgggctgact ataccttctt tgacggtccg
    12181 ctttcaggtc tgacgctggg caccggtggt cgttatactg gctccagcta tggtgatccg
    12241 gctaactcct ttaaagtggg aagttatacg gtcgtggatg cgttagtgcg ttatgatctg
    12301 gcgcgagtcg gcatggcggg ctccaacgtg gcgctgcatg ttaacaacct gttcgatcgt
    12361 gaatacgtcg ccagctgctt taacacttat ggctgcttct ggggcgcaga acgtcaggtc
    12421 gttgcaaccg caacctttcg tttctaattt ctcttttggg gcacggattt ccgtgcccat
    12481 ttcacaagtt ggctgttatg caggaataca cgaatcattc cgataccact tttgcactgc
    12541 gtaatatctc ctttcgtgtg cccgggcgca cgcttttgca tccgctgtcg ttaacctttc
    12601 ctgccgggaa agtgaccggt ctgattggtc acaacggttc tggtaaatcc actctgctca
    12661 aaatgcttgg tcgtcatcag cctccgtcag aaggggagat tcttcttgat gcccagccgc
    12721 tggaaagctg gagcagcaaa gcgtttgccc gcaaagtggc ttatttgccg cagcagcttc
    12781 ctccggcaga agggatgacc gtgcgtgaac tggtggcgat tggtcgttac ccgtggcatg
    12841 gcgcgctggg gcgctttggt gcggcagatc gcgaaaaggt cgaggaagct atctcgctgg
    12901 ttggcttaaa accgctggcg catcggctgg tcgatagtct ctctggcggc gaacgtcagc
    12961 gggcgtggat cgccatgctg gtggcgcagg atagccgttg tctgttgctc gacgaaccga
    13021 cttcagcgct ggatatcgcc caccaggttg atgtgctggc gctggtgcac cgtttaagtc
    13081 aggaacgtgg cctgacggtc attgccgtgt tgcacgatat caatatggcg gcacgctact
    13141 gtgattatct ggtcgccctg cgcagtggtg aaatgattgc tcagggaacg cctgcggaaa
    13201 ttatgcgcgg cgaaaccctc gaaatgattt atggcatccc gatgggtatt ttgccgcatc
    13261 cggcgggtgc tgcacctgtg agttttgttt attgatgagc ggcttacctc ttatttcgcg
    13321 ccgtcgactg ttaacggcga tggcgctttc tccgttgtta tggcagatga ataccgccca
    13381 cgcggcggct attgacccca atcgtattgt ggcgctggag tggttgccgg tggaattact
    13441 gctggcgctc ggcatcgtgc cttacggcgt ggcggatacc atcaactatc gcctgtgggt
    13501 cagcgaacca ccattgccgg actcagtgat tgacgtcggt ttgcgtacag aacctaacct
    13561 tgaactgctg accgaaatga aaccgtcgtt tatggtctgg tcggcaggat atggcccttc
    13621 atcagaaatg ctggcacgta ttgcgccggg tcgcggattt aacttcagtg acggcaaaca
    13681 tccgttggcg atggcgcgta aatcgctgac ggaaatggca gatttactta acctgcaaag
    13741 cgcagcggaa acgcatttag cgcaatacga agactttatc cgcagcatga aaccccgctt
    13801 tgtgaagcgt ggtgcacgcc cgttactgct gacgacgctt atcgatccgc gccatatgct
    13861 ggtcttcggt ccaaacagct tgttccagga aattctcgat gagtacggca tcccaaatgc
    13921 ctggcaaggg gaaaccaact tctggggcag taccgccgtc agtatcgatc gtctggcagc
    13981 gtataaagac gttgatgtgc tctgttttga tcacgacaac agcaaagaca tggatgcgct
    14041 aatggcaacc ccgctgtggc aggccatgcc gtttgtccgt acagggcgct ttcagcgcgt
    14101 acccgcagtc tggttttatg gtgcaacgct ttcggcaatg cactttgtgc gtgttctgga
    14161 taacgccatc ggaggtaaag cgtgagtaaa cgaattgcgc ttttcccggc gttattgctg
    14221 gcgctgttag tgattgtcgc tacggcgctc acctggatga acttctcaca ggcgctgccg
    14281 cgcagtcagt gggcgcaggc cgcctggtcg ccggatattg acgtcatcga gcagatgatt
    14341 tttcactaca gcttgttgcc gcgtctggcg atttcgctgc tggtgggcgc gggcctgggg
    14401 ctggtgggcg tgctgtttca gcaagtactg cgtaacccgc tggcggaacc gacgacgttg
    14461 ggcgttgcaa caggcgcgca actggggatt accgtcacca cgctctgggc gatccctagc
    14521 gcgatggcga gccagtttgc tgcgctggca ggggcttgcg ttgttggctt aattgtcttt
    14581 ggcgtcgcgt gggggaaacg gctgtcgccg gtaacgctga ttttcgcggg gttggtagtg
    14641 agcctttatt gcggcgcaat caatcagtta ctggttatct tccatcatga ccagttgcaa
    14701 agcatgtttc tgtggagcac tggaacgctg acgcaaaccg actggggcgg tgttgagcgt
    14761 ttatggccgc agttgctggg cggtgtgatg ctgacgttgc tgctacttcg tccgttaacc
    14821 ctgatggggc ttgatgatgg cgtggcgcgc aatctcgggc tggccttgtc gcttgcgcgt
    14881 ctggcggcgc tgtcgctggc gattgtcatc agtgcgctgc tggtgaacgc tgtggggatt
    14941 atcggcttta tcgggttgtt cgcgccgctg ctggcaaaaa tgctgggggc gcggcgtctg
    15001 ctgccacgac tgatgctggc gtcgttgatt ggtgcgctga tcctctggct ttccgatcaa
    15061 atcatcctct ggctgactcg cgtgtggatg gaagtgtcca ccggttcggt cactgcgttg
    15121 atcggtgcgc cgctgctact gtggctgctg ccgcgtttac gcagcattag cgcgccggat
    15181 atgaaggtta acgatcgtgt cgcgactgaa cgccaacatg tgctggcgtt tgccctcgcg
    15241 ggcggcgtgc tgttgttgat ggctgtggtg gtggcgctgt cgtttggtcg tgatgcgcac
    15301 ggctggacgt gggcgagcgg ggcgttgctc gaggatttaa tgccctggcg ctggccgcga
    15361 attatggcgg cgctgtttgc gggcgtcatg ctggcggtag cgggctgtat tattcagcga
    15421 ctgaccggca acccgatggc aagcccggaa gtgctgggga ttagctccgg cgcggcgttt
    15481 ggtgtggtgt taatgctgtt tctggtaccg ggtaatgcct ttggctggtt gttacccgca
    15541 ggcagtctcg gggcggcggt gacgctgttg atcattatga tcgccgccgg acgcggtgga
    15601 ttttccccac accgcatgtt actggcgggg atggcgttaa gtaccgcgtt caccatgctt
    15661 ttgatgatgt tgcaggcaag tggtgacccg cgaatggcgc aagtgctgac ctggatttcc
    15721 ggttcgacct acaacgcgac cgatgctcag gtctggcgca ccggaattgt gatggtgatt
    15781 ttgctggcga ttactccgct gtgccgccgc tggctgacca ttttaccgct gggtggtgat
    15841 accgcgcggg cggtagggat ggcgctgacg ccgacgcgaa ttgcgctgct gctgttagcg
    15901 gcttgcctga cggcgaccgc gacgatgact attggaccgt tgagttttgt tggtttaatg
    15961 gcaccgcata ttgcgcggat gatgggcttt cgacggacga tgccacacat cgtaatttcg
    16021 gcgctggtgg gtggtttact gctggtgttc gctgactggt gtgggcggat ggtgctgttt
    16081 ccattccaga tcccggcggg gctgctgtcg acctttatcg gtgcgccata ttttatctat
    16141 ttgctgagaa agcagagccg ttaatagaat gcctcgcccg gtgggcgagg caaaattatc
    16201 acaacttcgc gaacacccga cgtgcagcat cgatggtgtt attgatatct tccatgctgt
    16261 gcgccacaga cataaagcct gcttcaaacg ctgacggtgc caggtaaaca ccttcatcca
    16321 gcatcatatg gaagaaacgc ttaaaacgct cgacgtcaca ggccatcaca tcctgatagc
    16381 acgtcacgga ctcggcgtcg gtaaagaaga taccgaacat accaccgacg tggttaacga
    16441 ccagcggaat tcccgcttct tctgccgctt ccagcagacc ttctgccaga cgtgttgtca
    16501 gctcatccag cgtttcgtga atgcccggct gcgcgacttc attcagacag gcgaaacccg
    16561 ctgccatcgc aatcgggtta ccggaaagcg tacccgcctg atagaccgga cctgtcgggg
    16621 ccagcgcatc cattacatca cgacgaccac cgaatgcgcc taccggcatt ccaccgccga
    16681 tgatttttcc gaggcaggtt aaatctggca ctacggcgaa ataatcctgt gcgccagcta
    16741 gcgctacgcg gaaaccggtc atcacttcat cgatgatcag caacgcgcca aattcgtcgc
    16801 acagcgcacg cagacctggc aggaactctg gcagcggcgg aacacagttc atattgcctg
    16861 ccaccggctc gacgataata caggcaatct cttgcgggta ttgctcaaac gcggcgcgta
    16921 cagaggccag atcgttataa gtacaggtta aggtatgttt ggcgaaatct gccggaacgc
    16981 ccggcgagtt tggctggcct aacgtgagtg cgccagaacc ggctttcacc agcaggcagt
    17041 cagcgtgacc gtggtaacaa ccttcaaatt taatgatttt gtcgcgaccg gtaaaaccac
    17101 gggccaggcg gatggcgctc atcgtcgcct cggtgccgga gttcaccatg cgcaccatat
    17161 ccatggtcgg gaccagttcg gtcaccagtt gcgccatttt cacttccatt tcggtcggtg
    17221 caccaaagct taaaccacgc tcggcggctt caatcacggc attgcggatc gccggatggt
    17281 tatggcccag caccatcggc ccccaggaac cgacataatc gatataggct ttgccatcaa
    17341 catcgtacag ataagcgccg tccgcttttt cgataaacag tggagtgccg cccacgccag
    17401 taaaggcgcg aacaggggag ttcacaccgc cagggatcag ctcgcgcgct gcgctgtaaa
    17461 gattttcaga cttactcatg gagggttcct gattcgtaga aaaagtgaat ggctgctatt
    17521 ccatgttatt cataacaagt taaatactcg tcaaacatca ggctgcttgt actggtgcaa
    17581 tcctgatttc ttagagtata aaagttttgt gcatttgaaa cattacgctt tgcaaaggat
    17641 tttcatggaa cgtgcgagta aaatgccgtc atcttatttg tatgaccaat aagtgatcat
    17701 tggatgaaaa ctgatactcc ctctttagaa acaccgcagg ccgcgcgcct gcgacgcaga
    17761 caactgattc gccaacttct tgagcgcgat aaaaccccgt tagccatttt gtttatggcg
    17821 gcagtcgtcg gcacgcttgt cgggctggct gcggttgctt ttgacaaagg tgtcgcctgg
    17881 ttgcagaacc aacgtatggg ggcgctggta catactgctg ataattatcc gctactgtta
    17941 accgtcgctt ttctctgttc ggcggtgctg gcgatgttcg gctacttttt ggtgcgcaaa
    18001 tacgcgcccg aagcaggggg atcggggatc ccggaaattg aaggggcgct ggaagatcaa
    18061 cgtcctgttc gctggtggcg tgtattgccg gtgaaattct ttggcgggct ggggacactc
    18121 ggcggaggca tggggctagg gcgcgaagga ccaaccgtgc agatcggcgg caatatcagt
    18181 cgcatggtgc ttgatatttt ccgcctgaaa ggtgatgaag ctcgccatac gctgctggca
    18241 accggtgctg ctgcggggct ggctgcggcc tttaacgcgc cgctggcggg tattttgttt
    18301 attatcgaag agatgcgtcc gcagtttcgc tatacgttaa tttcgattaa agcggtattt
    18361 attggcgtca ttatgtcgac cattatgtac cggattttta atcatgaagt tgcgttgatt
    18421 gacgtcggta aactttctga tgcgccgctt aatacgctgt ggctttatct gatcctcggt
    18481 attatttttg gcattttcgg ccctattttt aataaatggg tgctggggat gcaggatttg
    18541 ctgcaccgtg tgcacggcgg caatattacc aaatgggtac tgatgggggg ggcgattggc
    18601 ggtctgtgtg gattgctggg gtttgtggca ccagcaacgt cgggcggcgg ttttaacctg
    18661 attcctatcg ctaccgcggg gaatttcagc atgggaatgc tggtgtttat cttcgtcgct
    18721 cgggtcatta ccaccttact ctgcttctct tccggcgcgc cgggcggtat ttttgccccg
    18781 atgctggcgc tgggtactgt gctgggaacc gctttcggaa tggttgccgt tgagctgttt
    18841 ccgcaatatc acctggaggc ggggacgttt gctattgccg gaatgggggc attactggcg
    18901 gcatctattc gcgcgccgtt aacggggatc attctggttc tggagatgac cgataactac
    18961 cagctcattt tgccaatgat tattaccggt cttggcgcaa cactattagc gcaatttacc
    19021 ggcgggaaac ctctatactc ggcgattctt gcgcgcacgc tggcaaaaca ggaagctgag
    19081 caactggcgc gaagcaaggc cgcatcagcc agcgagaata cttgaacgaa ataccagggt
    19141 attagataat ggcgattatt attgggttag aatttgcccg attgccgatg tcgtttggag
    19201 caaaatatga gtgatgacgt agcactgccg ctggagttta ccgacgcagc agccaacaaa
    19261 gttaaaagcc tgatcgctga cgaagataac ccgaatctga aattacgcgt gtatatcacc
    19321 ggtggcggtt gcagcggctt ccagtatggt ttcacctttg atgatcaggt gaacgaaggc
    19381 gatatgacca tcgaaaaaca gggcgttggc ctggtggttg atccgatgag cctgcaatat
    19441 ctggtcggcg gttccgttga ctacaccgaa ggactggaag gttctcgttt catcgtgacc
    19501 aacccgaacg cgaaaagcac ctgcggttgc ggttcttcct ttagcatcta atctgttgtt
    19561 catgtgccgg ataaactatc cggcacatct tctctcttca acgcccattc tcatccagcg
    19621 caaacgtcgg tagcttaaga tgccaacgaa tagccgccag ccgaatcaat agcgtcacga
    19681 ccatgcccat catactggct gtttccagtg gtacggaaaa tgtgtaataa gccgtagcgt
    19741 ggacaatacc gccgataata caggcagttg cgtagatttc tgtacgtaaa atcatgggga
    19801 tttcgcggac cagtacatca cgaatgatcc cgccgccaac gccagtaatg acgcccatac
    19861 aaaccgcgat taacggaccg gcttccgcat taaaggcttt attcacgcta atgccgacaa
    19921 acaccgccag accaacggcg tccagcaccg gcaacatcca ttttggtaag cgtcttggct
    19981 ggcgcaccag cacgatggtc agcatgctgg tgaccatggc aacgaccaga tcggtgggat
    20041 ctttcaccca aaataccggg ccgtgatcca gcgccatgtc gcgaattgtt ccgtcgccta
    20101 ctgcggtaac cacgcccagt accagaacac caaaagggtc catacgcaat tttccggcta
    20161 acaaaacgcc gtagatggca aataccgctg tgccgactat atccagccaa tagacgagca
    20221 ttgttcaatc cccactgagc acctgaaaag gtcaggcact aatctacctg tgaaagcgca
    20281 ttacagagct gttgtgctgc gaggataata cgtgggcttg cacgttcaaa ccagtcactc
    20341 gtgagaggaa taacgggaat tttgagctgt tcaccccagt attgtttgat tttaggaatt
    20401 tggtccggtc cgcctgtaat gacaatagcc tgtggcgagc gtgctaacac ctgttcgcgg
    20461 ctaacttgcg gccagggaac ccggctgtct ttaaagatgt tttctccgcc acaaacttcg
    20521 agtacctggt tctgaatcga ctcttttcca ctggtaaatg gcggattaat gccgaattgc
    20581 agaaaaacac gttttttagg tttatcagca tattgcgctt tcaattgcac gtactgatcc
    20641 agcagggatt gcgcggcttg ttcggccttg tctggttgcg gactccaggg ggccagttga
    20701 cgtaacgcat tggcaatttg ttcaatgctt gtcgcatcga cccacatcac ttttattccc
    20761 agcgaagcca gctggtcaac ctgccgctcg gcattacctc cacgccaggc aatcaccaga
    20821 tcgggtttca gcgcgacaat gcgttccaga ttcatcccct gccaggtgga aacctgctca
    20881 atcttttgcg cttgtggagg atagtcggaa tagctgctga ccccaaccgg cgtgatcccg
    20941 gcggcaaagg caagttcagt gttggcggga gaaagcgtga tgacgcgcgg cgcggcgttg
    21001 agccacagtg gcgcgagaaa agacagggcg accagcgccc tgaacagtga tttagccatg
    21061 tgccagtttc tgcaccagtg actcaaccat caggctggac tgtttagcgg caacagccag
    21121 gaactcatcg aagctaagat gagactgttg atcggccacg tcggagatgg cgcgtacgac
    21181 aacaaacggg acgttgaaat tgtggcagac atgggcgatt gccgtcgctt ccatctctac
    21241 agcaatggcc tgtgggaagt tgtggcggat tttcgccaga ccaacagaac cgttgatgaa
    21301 agcgtcgccg ctaacaatca ggccacgtac agcgttaaga ttcagttcgg caatgcaggc
    21361 ctcagcggca gcgatcagtt tatcgtcagc tttaaagcct gccggacagc ctggtaactg
    21421 accgtattca taaccaaatg ccgtgacatc cgcgtcgtga taacgcgctt cgtccgagac
    21481 aacgatatcg cccactttca gcgttggtgc caggccaccg gcagaaccgg tgttaataat
    21541 cacatctggc ttgcagtgtt ccaacagcag agtggcaccc agcgccgcag cgactttacc
    21601 gatgcccgat ttcagaagcg caacctcggt tccattcagt tggccggtat agatttcgca
    21661 accgccgaga ctgatagttt gacggttttc gattttgtca cgcagcagcg taacttcttc
    21721 ttccattgca ccaatgatgc cgattttcat agatttactc gcgataagcc cgatttgaag
    21781 gcatagttta ccatgcgctt acggggaagc gtatttctca cgcgggagag gacatggcac
    21841 agattgattt ccgaaaaaaa ataaactggc atcgtcgtta ccgttcaccg cagggcgtta
    21901 aaaccgaaca tgagatcctg cggatcttcg agagcgatcg cgggcgtatc atcaactctc
    21961 cggcaattcg tcgtctgcaa caaaagaccc aggtttttcc actggagcgc aatgccgccg
    22021 tgcgcacgcg tcttacccac tcgatggaag tccagcaggt gggacgctac atcgccaaag
    22081 aaattttaag ccgtctgaaa gagcttaaat tgctggaagc atacggcctg gatgaactga
    22141 ccggcccctt tgaaagcatt gttgagatgt catgcctgat gcacgatatc ggcaatccgc
    22201 cgtttggtca ttttggcgaa gcagcgataa atgactggtt tcgccaacgt ttgcacccgg
    22261 aagatgccga aagccagcct ctgactgacg atcgctgcag cgtggcggca ctacgtttac
    22321 gggacgggga agaaccgctt aacgagctgc ggcgcaagat tcgtcaggac ttatgtcatt
    22381 ttgaggggaa tgcacaaggc attcgtctgg tgcatacatt gatgcggatg aatctcactt
    22441 gggcacaggt tggcggtatt ttaaaatata cccgtccggc gtggtggcgt ggcgaaacgc
    22501 ctgagacaca tcactattta atgaaaaagc cgggttatta tctttctgaa gaagcctata
    22561 ttgcccgctt gcgtaaagaa cttaatttgg cgctttacag tcgttttcca ttaacgtgga
    22621 ttatggaagc tgccgacgac atctcctatt gtgtggcaga ccttgaagat gcggtagaga
    22681 aaagaatatt taccgttgag cagctttatc atcatttgca cgaagcgtgg ggccagcatg
    22741 agaaaggttc gctcttttcg ctggtggttg aaaatgcctg ggaaaaatca cgctcaaata
    22801 gtttaagccg cagtacggaa gatcagtttt ttatgtattt acgggtaaac accctaaata
    22861 aactggtacc ctacgcggca caacgattta ttgataatct gcctgcgatt ttcgccggaa
    22921 cgtttaatca tgcattattg gaagatgcca gcgaatgcag cgatcttctt aagctatata
    22981 aaaatgtcgc tgtaaaacat gtgtttagcc atccggatgt cgagcagctt gaattgcagg
    23041 gctatcgggt cattagcgga ttattagaga tttatcgtcc tttattaagc ctgtcgttat
    23101 cagactttac tgaactggta gaaaaagaac gggtgaaacg tttccctatt gagtcgcgct
    23161 tattccacaa actctcgacg cgccatcggc tggcctatgt cgaggctgtc agtaaattac
    23221 cgtcagattc tcctgagttt ccgctatggg aatattatta ccgttgccgc ctgctgcagg
    23281 attatatcag cggtatgacc gacctctatg cgtgggatga ataccgacgt ctgatggccg
    23341 tagaacaata accagtcttt tgtaaagacg aacaataaat ttttaccttt tgcagaaact
    23401 ttagttcgga acttcgggct ataaaacgaa tctgaagaac acagcaattt tgcgttatct
    23461 gttaatcgag actgaaatac atgaaaaaaa acacattagc actgagtgca ctggctctga
    23521 gtttaggttt ggcgttatct ccgctctctg caacggcggc tgagacttct tcagcaacga
    23581 cagcccagca gatgccaagc cttgcaccga tgctcgaaaa ggtgatgcct tcagtggtca
    23641 gcattaacgt agaaggtagc acaaccgtta atacgccgcg tatgccgcgt aatttccagc
    23701 agttcttcgg tgatgattct ccgttctgcc aggaaggttc tccgttccag agctctccgt
    23761 tctgccaggg gggccagggc ggtaatggcg gcggccagca acagaaattc atggcgctgg
    23821 gttccggcgt catcattgat gccgataaag gctatgtcgt caccaacaac cacgttgttg
    23881 ataacgcgac ggtgattaaa gtccagctga gcgatggtcg taagttcgac gcgaagatgg
    23941 ttggcaaaga tccgcgctct gatatcgcgc tgatccaaat ccagaacccg aaaaaactga
    24001 ccgcaattaa gatggcggat tctgatgcac tgcgcgtggg tgattacacc gtagcgattg
    24061 gtaacccgtt tggtctgggc gagacggtaa cttccgggat tgtctctgcg ctggggcgta
    24121 gcggcctgaa tgctgaaaac tacgaaaact tcatccagac cgatgcagcg atcaaccgtg
    24181 gtaactccgg tggtgcgctg gttaacctga acggcgaact gatcggtatc aacaccgcga
    24241 tcctcgcacc ggacggcggc aacatcggta tcggttttgc tatcccgagt aacatggtga
    24301 aaaacctgac ctcgcagatg gtggaatacg gccaggtgaa acgcggtgag ctgggtatta
    24361 tggggactga gctgaattcc gaactggcga aagcgatgaa agttgacgcc cagcgcggtg
    24421 ctttcgtaag ccaggttctg cctaattcct ccgctgcaaa agcgggcatt aaagcgggtg
    24481 atgtgatcac ctcactgaac ggtaagccga tcagcagctt tgccgcactg cgtgctcagg
    24541 tgggtactat gccggtgggc agcaaactga ccctgggctt actgcgcgac ggtaagcagg
    24601 ttaacgtgaa cctggaactg cagcagagca gccagaatca ggttgattcc agctccatct
    24661 tcaacggcat tgaaggcgct gagatgagca acaaaggcaa agatcagggc gtggtagtga
    24721 acaacgtgaa gacgggcact ccggctgcgc agatcggcct gaagaaaggt gatgtgatta
    24781 ttggtgcgaa ccagcaggca gtgaaaaaca tcgctgaact gcgtaaagtt ctcgacagca
    24841 aaccgtctgt gctggcactg aacattcagc gcggcgacag caccatctac ctgttaatgc
    24901 agtaatctcc ctcaacccct tcctgaatac gggaaggggt tctccttaca atctgtgaac
    24961 ttcgccacaa ctccatacat cttcatcatc ctttgggcat ttgcacaatg ccgtacgtta
    25021 cgtacttcct tatgctaagc cgtgcataac ggaggactta tggctggctg gcatcttgat
    25081 accaaaatgg cgcaggatat cgtggcacgt accatgcgca tcatcgatac caatatcaac
    25141 gtaatggatg cccgcgggcg aattatcggc agcggcgatc gtgagcgtat tggtgaattg
    25201 cacgaaggtg cattgctggt actttcacag ggacgagtcg tcgatatcga tgacgcggta
    25261 gcacgtcatc tgcacggtgt gcggcagggg attaatctac cgttacggct ggaaggtgaa
    25321 attgtcggcg taattggcct gacaggtgaa ccagagaatc tgcgtaaata tggcgaactg
    25381 gtctgcatga cggctgaaat gatgctggaa cagtcgcggt tgatgcactt gttggcgcag
    25441 gatagccgtt tgcgggaaga actggtgatg aacctgattc aggcagagga gaatactccc
    25501 gcacttctga atgggcgcaa cggctgggga tcgatctcaa tcaaccgcga gtggtggcta
    25561 ttgttgaggt cgacagcggt cagcttggcg tggacagcgc aatggcggag ttacaacaac
    25621 tgcaaaacgc gctgactacg cccgagcgta ataatctggt ggcgattgtc tcgctaaccg
    25681 aaatggtggt gttgaaaccg gcgttgaact cttttgggcg ctgggatgca gaagatcatc
    25741 gtaagcgagt tgaacaactg attacccgca tgaaagagta cggccagctg cgttttcgcg
    25801 tttcactggg caactatttt accggtcctg gcagtattgc ccgatcctat cgtacggcga
    25861 aaacgacgat ggtggtgggt aaacagcgga tgccagaaag tcgctgctat ttttatcagg
    25921 atctgatgtt acctgtgtta ctcgacagtt tgcgtggcga ctggcaggcc aacgaactgg
    25981 cgcgaccgct ggcgcggctg aaagcgatgg acaataacgg cttgctgcga cgaacgctgg
    26041 cggcgtggtt tcgccacaat gtgcaaccgc tggcaacgtc aaaggcgttg tttattcatc
    26101 gtaataccct ggagtatcgg cttaatcgta tatcggaact gaccgggctt gatttgggca
    26161 attttgatga caggttgctg ctgtatgtgg cgttacaact ggatgaagag cggtagatta
    26221 tgcgttaagg ttgaggcggc gctggctcat tcccctcagc ccgaagggga gaggggaatg
    26281 cccgagttaa ccgtcaattc gggattattt acgcgttagt ttttccagat cggcttcaat
    26341 ctcgctgatc ttattggtta cgactgactc caggtgacgt aagtcgtcga ggatcttacg
    26401 cttaagatca acttcgctgc ggtcacgctg acagatttga tcaagctcat caatgatata
    26461 ccgtagattc gggctgattt cctggacttc tttataaccc tgacccacac catcagcgac
    26521 gaccgtttta cgctgacgcg gatatttaaa cttaacgctc ttggcgaaaa actcgccttt
    26581 gtctttctgg aaatagattt tcagaatatc gttgttggct tcctgccgga ggctgtaacg
    26641 atcaatttct tcaggattgg taatacccag acttttcaga ttgtcgtaca tagcgttacc
    26701 tcaaaatgag tcagtaaatt gtgcttattt tagcatttgg cttggcccgc ccggctcgac
    26761 tgtgatcgca gaaagccttt ttcttccaga cttctatcat ggcgtaaaaa tcaaaaaatt
    26821 acctgcttta ttgtggcgat attccatggg atggattagc acatttatct tcgttttgcg
    26881 aattacatga cagtccgaaa caagatgaaa aagtatttaa aaaaggggga ggaagaatga
    26941 gtaggacgaa ttattctgag ccagaaagca tacgggcagc atcatgctgc ccgtatatgt
    27001 actcttaatc gatggtacgc agcagttcgt taatgccgac tttgccgcga gttttcgcgt
    27061 caactttctt aacgataacc gcacagtaga ggctgtattt gccatctttt gacggcagat
    27121 tacctgaaac aaccacagac cccgccggaa cgcgaccgta gtggatttcg ccggtttcac
    27181 ggtcgtaaat acgggtgctc tgaccaatgt atacgcccat agagatgacg gaaccttctt
    27241 cgacaatcac cccttcaacc acttcagagc gtgcgccaat gaagcagtta tcttcaataa
    27301 tggtcgggtt agcctgcagc ggctccagca cgccgccgat gcccacgcca ccggaaaggt
    27361 ggacgttttt accaatctgc gcacaagaac cgacggtcgc ccaggtatca accatggtgc
    27421 cttcatcaac atatgcgccg atgttaacgt aagacggcat cagcacggtg ttacgggcaa
    27481 taaacgcacc ctgacgtacc gccgctggtg gcacaacgcg gaagccttct ttctggaaac
    27541 gtgcttcgtc gtagtcggcg aatttcatcg gcactttgtc gaagtagcgg ctttctgccc
    27601 cttcgatcac ctgattatca ttaatacgga aagagagcag caccgctttt ttcaaccact
    27661 gatgcgtcac ccactgaccg tcaatttttt ccgctacacg cagtgcgccg gaatccagca
    27721 gggcgatcac ctgatttacc gcttcgcggg taacggtgtc tgcattggct ggcgtgatct
    27781 cggcacggcg ttcaaaagcg gtttcaataa tgttctgtaa ctgctgcatt gttaaactct
    27841 tttcatatca gtaaacacat caccctttat cgtttggatt gagggcctct gtcaaccgct
    27901 gatgcacttc ctgctgcaac tcgttattaa gcgcacgccg gtcagcggtg gcaattatga
    27961 ataaatcttc tactcgctcg ccaatggttg taattcgggc accatgaagc gaaattccca
    28021 gatcggcaaa aattttcccg actcgcgcca gcagtccagg ttggtcgagg gcgatcagtt
    28081 cgaggaacga tttgcggtcg gtatgggtcg gcaaaaacgt tacttcggtt tcaacagtaa
    28141 aatggcgtaa tttggcgggt tggcgacggg gctgcggtgg ctgccagcta ctttgcgtca
    28201 gcacctgctc cagaccaaac cgaataacct catggcgatc tgcggacagc gggctgccat
    28261 cgggttccag cacgataaag gtatccatcg ccataccgtc gcgagtggtg aaaatttgtg
    28321 cgtcgtgaac acttaaattg cggcggtcta attcggcaca gacggcggca aacagatagg
    28381 ggcggtccgg gctccagata aaaatctcgg tgcctccacg cgtagcctgc gggctaagca
    28441 ataccagcgg tttgcttaaa tcatgctgta ataaatggcg ggcatgccag gccagttgat
    28501 ttgggctatg gcggacaaaa tagttagcac gacagcgtga ccaaatttgg tgcagcgcct
    28561 cttcgtcgat gttatccatg cgcagtagtg ccagtgcctg gagttggtga tggcgaaccc
    28621 gttcgcgcat atccggcgtg ttttgcattc cgcgtcgtag ctgcttttcg gtggcaaaat
    28681 agagttcgcg caacagactt tgcttccagc tattccacag tgtttcgttg gtggcgcaaa
    28741 tgtcagccac tgtcaggcat accagatagc gcagacgatt ttccgtttga acttcttcgg
    28801 caaactgctt gatgacttca gggtcctgaa tatcgcggcg ttgtgctgtc accgacatca
    28861 gcaagtgctg gcgaaccagc caggcaacca gctgtgtttc gcgtgagttc agcccgtgga
    28921 gttctgcaaa atgcactaca tcctgagcac cgagaatgga gtggtcgccg ccgcgtcctt
    28981 tggcgatatc gtgaaacagc gcggcgatga aaatcagctc agttgacggc aggcgcggcc
    29041 agacgtccac acacaacgga tggcgctggc gcgtttcttc actggcaaaa ctctccagtt
    29101 tcagcatcac gcggatagta tgttcatcca ccgtgtaggc gtggaacaga tcaaactgca
    29161 tctgcccgac gatatgcgac cattgcggca tatacgcgcc gagcacgcta tggcgatgca
    29221 ttggcaatag tccgcgccgc accgctccgg ggtgacgcag aatgctcaaa aacagttttc
    29281 gtgcttgcgg aatattacac agcggttgtt gcagatggcg acgggcatgg cgtaactggc
    29341 gcagcgtggt ggagtaaatg ccggtgatcg cactgttgcg caccatggtg tagaacatac
    29401 gcaagatggc ttccggctgg cgcataaata gtgtttcatc acgcaggtcg attagcgtac
    29461 cgcgtagctg aaactcatcg tcgagtggac gtggtttttc gtcggcggga agggcgagga
    29521 tggcttcatc gaacagttgc agcagcatct ggttgagttc actgacgcgg cgtgtaacgc
    29581 ggaagtaatc cttcatcatc cgctcgaccg gttcgttgcc ttcaccactg taattcagac
    29641 gctgagcgac gctaagttgg cgatcgaata acagacgatt atcgtaacgg ctgacgacca
    29701 gatgcagggc aaagcgaata cgccacagta tatgcagaca ttcgtttaat tccgcccgct
    29761 ccgctgaggt taagaagcca aacccgacca tttcatccag cgatgttgcg ccaaaatgac
    29821 ggcgggccac ccattgcaga gtgtgaatat cgcgcaagcc gccagggcta cttttgatgt
    29881 ctggttcaag gttgtagctg gtgccatggt aacgctgatg gcgctggttc tgttcttcaa
    29941 ctttcgctgc gtagaacttg tcggaaggcc agaatccttc gctaaaaata tgtttttgca
    30001 gttcgaggaa gagcgcaaca tcgccaatta ataagcggga ttcgattaaa ttggtggcga
    30061 cggttaaatc cgataaccct tccaacatgc actcttcaag cgtgcgcaca ctatgaccga
    30121 cttccagctt tacatcccag agcagcgtta acagctcgcc cactttttgc gcctgatcgt
    30181 ccgggagctt tttacggctt aaaatcagta aatcgatgtc agaaagtgga tgcagctcgc
    30241 cacgaccgta gccaccgacg gcaaccaatg ccaggtcggc aatctggctg aatcccgctt
    30301 caatccataa tcgttgcagg agctggtcta tgaactcggt acgtgcctca atcaactgtt
    30361 ctgcagagat cccattgtca aaggcatcac ccagccaacg ctggaaagta tcgatatggg
    30421 cttttatccc acagacggtt aattcatcac ggggccaggc gcatggattt tgcggttgac
    30481 cgggcagggt ggagagagcg gtgtttgcgt actgttctgg aagggtattc attgtgcgcc
    30541 acccataaga ttaaattatc gcattaaaaa agccggcatt cgccggcttc atcttattcg
    30601 tcgtgcgaga ttatcgccgg gatggtgtca tccttgcgta gcgtcaaaat ttcgcagccg
    30661 ttatcagtca ccacaatagt atgctcatat tgtgcagaca agctgcgatc tttggttttt
    30721 accgtccagc catctttcat ggtgcggatc tcttttttac ccgcgttgac cattggctca
    30781 atggtgaacg tcatccccgg tttcagcacg acgttggttt cacgggagtc atagtgcagc
    30841 acctgcggtt cttcatggaa gccgcgacca ataccgtgtc cgcaatattc acgaacgacg
    30901 gagaagcctt ctgcttcgac aaatttctga atcgccgcac cgatttcgcg cagattaatg
    30961 cctggtttta ccatgcgtag cgccaggtac aggctttctt gcgtgatgcg gcacaggcgt
    31021 tcgcccatga tggtcggctt accgacgata aacattttcg aggtatcgcc gtggaaacca
    31081 tctttgatta cggtgacatc aatgttaacg atatcgccat ctttcagcag cttagcatcg
    31141 tccgggatac cgtggcacac cacttcatta atagagatgc aaacggattt cggatagccg
    31201 tgatagccga ggcaggcaga aaccgcgtgt tgttcattaa caatgtaatc attacagatg
    31261 cgatccagct cgccggtgct gacgcccggt ttaacatacg gttcgatcat ctccagcact
    31321 tcggcagcca gtcggccagc gacgcgcatt ttttcgatat cttctggggt cttgattgag
    31381 atagccatta attctgtcca tcagcgtcgg tgataccgac aatatatatg taagtgccgt
    31441 caatggtatc acacccgggc aaattgagaa tcattctgaa tttcgccaaa cgtgccactg
    31501 aaggttttct ataatagaaa attcgacgtc tgatgctgta cacagcgcca acaattattg
    31561 gtgtccacga cgtatttgtg gtataaagcg cgccggactt ccgatccatt tcgtatacac
    31621 agactggacg gaagcgacaa tctcactttg tgtaacaaca cacacgtatc ggcacatatt
    31681 ccggggtgcc ctttggggtc ggtaatatgg gatacgtgga ggcataaccc caacttttat
    31741 atagaggttt taatcatggc aactgtttcc atgcgcgaca tgctcaaggc tggtgttcac
    31801 ttcggtcacc agacccgtta ctggaacccg aaaatgaagc cgttcatctt cggtgcgcgt
    31861 aacaaagttc acatcatcaa ccttgagaaa actgtaccga tgttcaacga agctctggct
    31921 gaactgaaca agattgtttc tcgcaaaggt aaaatccttt tcgttggtac taaacgcgct
    31981 gcaagcgaag cggtgaaaga cgctgctctg agctgcgacc agttcttcgt gaaccatcgc
    32041 tggctgggcg gtatgctgac taactggaaa accgttcgtc agtccatcaa acgtctgaaa
    32101 gacctggaaa ctcagtctca ggacggtact ttcgacaagc tgaccaagaa agaagcgctg
    32161 atgcgcactc gtgagctgga gaaactggaa aacagcctgg gcggtatcaa agacatgggc
    32221 ggtctgccgg acgctctgtt tgtaatcgat gctgaccacg aacacattgc tatcaaagaa
    32281 gcaaacaact tgggtattcc ggtatttgct atcgttgata ccaactctga tccggacggt
    32341 gttgacttcg ttatcccggg taacgacgac gcaatccgtg ctgtgaccct gtacctgggc
    32401 gctgttgctg caaccgtacg tgaaggccgt tctcaggatc tggcttccca ggcggaagaa
    32461 agcttcgtag aagctgagta ataaggcttg ataactcccc caaaatagtt cgagttgcag
    32521 aaaggcggca agctcgagaa ttcccgggag cttacatcag taagtgaccg ggatgagcga
    32581 gcgaagacaa cgcatctgcg gcgcgaaata tgaaggggga gagcccttat agaccaggta
    32641 gtacacgttt ggttaggggg cctgcatatg gccccctttt tcacttttat atctgtgcgg
    32701 tttaatgccg ggcagatcac atctccgagg attttagaat ggctgaaatt accgcatccc
    32761 tggtaaaaga gctgcgtgag cgtactggcg caggcatgat ggattgcaaa aaagcactga
    32821 ctgaagctaa cggcgacatc gagctggcaa tcgaaaacat gcgtaagtcc ggtgctatta
    32881 aagcagcgaa aaaagcaggc aacgttgctg ctgacggcgt gatcaaaacc aaaatcgacg
    32941 gcaactacgg catcattctg gaagttaact gccagactga cttcgttgca aaagacgctg
    33001 gtttccaggc gttcgcagac aaagttctgg acgcagctgt tgctggcaaa atcactgacg
    33061 ttgaagttct gaaagcacag ttcgaagaag aacgtgttgc gctggtagcg aaaattggtg
    33121 aaaacatcaa cattcgccgc gttgctgcgc tggaaggcga cgttctgggt tcttatcagc
    33181 acggtgcgcg tatcggcgtt ctggttgctg ctaaaggcgc tgacgaagag ctggttaaac
    33241 acatcgctat gcacgttgct gcaagcaagc cagaattcat caaaccagaa gacgtatccg
    33301 ctgaagtggt agaaaaagaa taccaggtac agctggatat cgcgatgcag tctggtaagc
    33361 cgaaagaaat cgcagagaaa atggttgaag gccgcatgaa gaaattcacc ggcgaagttt
    33421 ctctgaccgg tcagccgttc gttatggaac caagcaaaac tgttggtcag ctgctgaaag
    33481 agcataacgc tgaagtgact ggcttcatcc gtttcgaagt gggtgaaggc atcgagaaag
    33541 ttgagactga ctttgcagca gaagttgctg cgatgtccaa gcagtcttaa ttatcaaaaa
    33601 ggagccgcct gagggcggct tctttttgtg cccatcttgt aaattcagct aacccttgtg
    33661 gggctgcgct gaaaagcgac gtacaatgtc gctagtatta attcatttca atcgttgaca
    33721 gtctcaggaa agaaacatgg ctaccaatgc aaaacccgtc tataaacgca ttctgcttaa
    33781 gttgagtggc gaagctctgc agggcactga aggcttcggt attgatgcaa gcatactgga
    33841 tcgtatggct caggaaatca aagaactggt tgaactgggt attcaggttg gtgtggtgat
    33901 tggtgggggt aacctgttcc gtggcgctgg tctggcgaaa gcgggtatga accgcgttgt
    33961 gggcgaccac atggggatgc tggcgaccgt aatgaacggc ttggcaatgc gtgatgcact
    34021 gcaccgcgcc tatgtgaacg ctcgtctgat gtccgctatt ccattgaatg gcgtgtgcga
    34081 cagctacagc tgggcagaag ctatcagcct gttgcgcaac aaccgtgtgg tgatcctctc
    34141 cgccggtaca ggtaacccgt tctttaccac cgactcagca gcttgcctgc gtggtatcga
    34201 aattgaagcc gatgtggtgc tgaaagcaac caaagttgac ggcgtgttta ccgctgatcc
    34261 ggcgaaagat ccaaccgcaa ccatgtacga gcaactgact tacagcgaag tgctggaaaa
    34321 agagctgaaa gtcatggacc tggcggcctt cacgctggct cgtgaccata aattaccgat
    34381 tcgtgttttc aatatgaaca aaccgggtgc gctgcgccgt gtggtaatgg gtgaaaaaga
    34441 agggacttta atcacggaat aattcccgtg atggataaat aagggtaaga ttccgcgtaa
    34501 gtatcgcggg ggcgtaagtc tggttataag gcgttattgt tgcaggcagt ttggtcacgg
    34561 ccagcgcgca gcaaccggag cgtacaaaag tacgtgagga tggcgagcac tgctcggggc
    34621 caaaatggca aataaaatag cctaataatc cagacgatta cccgtaatat gtttaatcag
    34681 ggctatactt agcacacttc cactgtgtgt gactgtctgg tctgactgag acaagttttc
    34741 aaggattcgt aacgtgatta gcgatatcag aaaagatgct gaagtacgca tggacaaatg
    34801 cgtagaagcg ttcaaaaccc aaatcagcaa aatacgcacg ggtcgtgctt ctcccagcct
    34861 gctggatggc attgtcgtgg aatattacgg cacgccgacg ccgctgcgtc agctggcaag
    34921 cgtaacggta gaagattccc gtactctgaa aatcaacgtg tttgatcgtt caatgtctcc
    34981 ggccgttgaa aaagcgatta tggcctccga tctcggcctg aacccgaact ctgcgggtag
    35041 cgacatccgt gttccgctgc cgccgctgac ggaagaacgt cgtaaagatc tgaccaaaat
    35101 cgttcgtggt gaagcagaac aagcgcgtgt tgcagtacgt aatgtgcgtc gtgacgcgaa
    35161 cgacaaagtg aaagcactgt tgaaagataa agagatcagc gaagacgacg atcgccgttc
    35221 tcaggacgat gtacagaaac tgactgatgc tgcaatcaag aaaattgaag cggcgctggc
    35281 agacaaagaa gcagaactga tgcagttctg atttcttgaa cgacaaaaac gccgctcagt
    35341 agatccttgc ggatcggctg gcggcgtttt gctttttatt ctgtctcaac tctggatgtt
    35401 tcatgaagca actcaccatt ctgggctcga ccggctcgat tggttgcagc acgctggacg
    35461 tggtgcgcca taatcccgaa cacttccgcg tagttgcgct ggtggcaggc aaaaatgtca
    35521 ctcgcatggt agaacagtgc ctggaattct ctccccgcta tgccgtaatg gacgatgaag
    35581 cgagtgcgaa acttcttaaa acgatgctac agcaacaggg gagccgcacc gaagtcttaa
    35641 gtgggcaaca agccgcttgc gatatggcag cgcttgagga ggttgatcag gtgatggcag
    35701 ccattgttgg tgctgctggg ctgttaccta cgcttgctgc gatccgcgcg ggtaaaacca
    35761 ttttgctggc caataaagaa tcactggtta cctgcggacg tctgtttatg gacgccgtaa
    35821 agcagagcaa agcgcaattg ttaccggtcg atagcgaaca taacgctatt tttcagagtt
    35881 taccgcaacc tatccagcat aatctgggat acgctgacct tgagcaaaat ggcgtggtgt
    35941 ccattttact taccgggtct ggtggccctt tccgtgagac gccattgcgc gatttggcaa
    36001 caatgacgcc cgatcaagct tgccgtcatc cgaactggtc gatgggacgt aaaatttctg
    36061 tcgattcggc taccatgatg aacaaaggtc tggaatacat tgaagcgcgt tggctgttta
    36121 acgccagcgc cagccagatg gaagtgctga ttcacccgca gtcagtgatt cactcaatgg
    36181 tgcgctatca ggacggcagt gttctggcgc agctggggga accggatatg cgtacgccaa
    36241 ttgcccacac catggcatgg ccgaatcgcg tgaactctgg cgtgaagccg ctcgattttt
    36301 gcaaactaag tgcgttgaca tttgccgcac cggattatga gcgttatcca tgcctgaaac
    36361 tggcgatgga ggcgttcgaa caaggccagg cagcgacgac agcattgaat gccgcaaacg
    36421 aaatcaccgt tgctgctttt cttgcgcaac aaatccgctt tacggatatc gctgcgttga
    36481 atttatccgt actggaaaaa atggatatgc gcgaaccaca atgtgtggac gatgtgttat
    36541 ctgttgatgc gaacgcgagt gaagtcgcca gaaaagaggt gatgcgtctc gcaagctgag
    36601 gataatccgg ctacagagag tcgcgctatt tgttagcgta gggcttcagt gatatagtct
    36661 gcgccatctg atcgtaagta gttggcttta taaggtcagg tatgccgtgg ttttacacgg
    36721 cttttttttg tataggcttc agtattcctg agtaccgtaa accctgtcag ggaataaaaa
    36781 acgcgtgatg ttgtctgcta ctcaaccact tagcgaaaaa ttgccagcgc atggctgccg
    36841 tcatgttgcg atcattatgg acggcaatgg tcgctgggca aaaaagcaag ggaagattcg
    36901 tgcctttggg cataaagccg gggcaaaatc cgtccgccgg gctgtctctt ttgcggccaa
    36961 caacggtatt gaggcgttaa cgctgtatgc ctttagtagt gaaaactgga accgaccagc
    37021 gcaggaagtc agtgcgttaa tggaactgtt tgtgtgggcg ctcgatagcg aagtaaaaag
    37081 tctgcaccga cataacgtgc gtctgcgtat tattggcgat accagtcgtt ttaactcgcg
    37141 tttgcaagaa cgtattcgta aatctgaagc gctaacagcc ggaaataccg gtctgacgct
    37201 gaatattgcg gcgaactacg gtggacgttg ggatatagtc cagggagtca ggcaactggc
    37261 tgaaaaggtg cagcaaggaa acctgcaacc agatcagata gatgaagaga tgttaaacca
    37321 gcatgtctgt atgcatgaac tggcccctgt agatttagta attaggactg ggggagagca
    37381 tcgcattagt aactttttgc tttggcaaat tgcctatgcc gaactttact ttacagatgt
    37441 tctctggccc gatttcgatg aacaagactt tgaaggggcg ttaaatgcct ttgctaatcg
    37501 agagcgtcgt ttcggcggca ccgagcccgg tgatgaaaca gcctgatggg ggtcgctttt
    37561 gctgaagtat cgcctgatat ctgcttttgt gttaataccc gtcgtcatcg cggcgttgtt
    37621 tctgttgccg ccggtggggt tcgccattgt aacgctggtg gtctgcatgc tggcagcgtg
    37681 ggaatgggga cagcttagcg gttttaccac tcgttcgcag cgagtatggt tggcggtgtt
    37741 atgcgggtta ttgttggcgc tgatgctttt tctgttgccg gaatatcacc gaaatattca
    37801 tcaaccgctg gttgaaatct cactttgggc ttcgttgggt tggtggattg tcgcgctatt
    37861 gctggtgctg ttttacccag gttccgcagc aatctggcgt aactctaaaa cattgcgcct
    37921 tatttttggc gtgctaacca ttgttccctt cttctggggc atgctggcgt tacgggcctg
    37981 gcactatgac gagaatcatt acagtggcgc aatatggctg ctctatgtca tgatcctggt
    38041 atggggcgct gactccggcg catatatgtt tggcaaattg tttggtaaac ataagctggc
    38101 accgaaggtt tctccgggta aaacctggca aggctttata ggtggactcg ctactgcagc
    38161 ggtaatctca tggggttatg gcatgtgggc gaatctcgac gtcgctcccg tcaccttact
    38221 catttgctct attgtcgcag cgttagcctc agtgctcggc gatctgaccg agagtatgtt
    38281 taagcgtgaa gcaggaatta aggacagcgg tcatttaatt ccaggacacg gtggtatttt
    38341 agatcgtatt gatagcctga cggctgcggt accggtcttt gcttgcttgt tgttactggt
    38401 attcaggacg ctttaacgga aggtaatatg ctgagttttc tctgggattt ggcttcgttc
    38461 atcgttgcac tgggtgtact tatcaccgtg catgaatttg gtcatttctg ggttgcccgg
    38521 cgttgtggtg ttcgcgttga gcgtttctca atagggtttg gtaaggcgct ctggcggcga
    38581 actgataagc tcggcaccga atatgttatc gccctgatcc cgttgggcgg ttatgtcaaa
    38641 atgctggatg agcgcgcaga accggtcgtt ccggaactcc gccaccatgc cttcaataat
    38701 aaatctgtcg gccaacgagc ggcgattatt gccgcaggtc cggttgcaaa cttcattttt
    38761 gctatctttg cctactggct ggtttttatt attggtgtgc ctggcgtacg tccggtggtt
    38821 ggcgaaatag cagccaattc gatagctgcg gaagcacaaa ttgcaccagg tacggaacta
    38881 aaagccgtag atggtatcga aacgcctgat tgggatgccg tgcgtttgca gttggtcgat
    38941 aaaattggcg atgaaagcac caccattaca gtagcgccat ttgacagcga ccaacggcgg
    39001 gatgtaaagc tcgatttacg tcactgggcg tttgagcctg ataaagaaga tccggtatct
    39061 tcgctgggga ttcgtcctcg tgggccgcaa attgaacctg tactggaaaa tgtgcagcca
    39121 aactcggcgg caagcaaggc aggtttgcaa gcaggcgaca ggatcgttaa agtcgatggt
    39181 cagcccttaa cgcagtgggt gacctttgtg atgcttgtcc gggataaccc gggtaaatcc
    39241 ttagcgttag aaatcgaaag gcaggggagt cccttgtctt tgacattaat cccggagagt
    39301 aaaccgggta atggtaaagc gattggtttt gtcggtattg agccgaaagt cattcctttg
    39361 ccagatgagt ataaagttgt acgccagtat gggccgttca acgccatcgt cgaagccacg
    39421 gacaaaacgt ggcagctgat gaagctgacg gtcagtatgc tgggaaaatt gatcaccggt
    39481 gatgtgaaac tgaacaacct cagtgggccg atctctatcg ccaagggggc tgggatgaca
    39541 gcggaactcg gggtagttta ttacctgccg tttcttgcgc ttattagcgt gaacttaggg
    39601 ataattaacc tgtttccgtt gcccgtactt gacggggggc atctgctgtt ccttgcgatc
    39661 gaaaagatca agggcggacc ggtatccgag cgggttcaag acttttgtta tcgcattggc
    39721 tcgattctgc tggtgctgtt aatggggctt gcacttttca atgatttctc tcggttatga
    39781 gagttagtta ggaagaacgc ataataacga tggcgatgaa aaagttgctc atagcgtcgc
    39841 tgctgtttag cagcgccacc gtatacggtg ctgaagggtt cgtagtgaaa gatattcatt
    39901 tcgaaggcct tcagcgtgtc gccgttggtg cggccctcct cagtatgccg gtgcgtacag
    39961 gcgacacggt taatgatgaa gatatcagta ataccattcg cgctctgttt gctaccggca
    40021 actttgagga tgttcgcgtc cttcgtgatg gtgataccct tctggttcag gtaaaagaac
    40081 gtccgaccat tgccagcatt actttctccg gtaacaaatc ggtgaaagat gacatgctga
    40141 agcaaaacct cgaggcttct ggtgtgcgtg tgggcgaatc cctcgatcgc accaccattg
    40201 ccgatatcga gaaaggtctg gaagacttct actacagcgt cggtaaatat agcgccagcg
    40261 ttaaagctgt cgtgaccccg ctgccgcgca accgtgttga cctaaaactg gtgttccagg
    40321 aaggtgtgtc agctgaaatc cagcaaatta acattgttgg taaccatgct ttcaccaccg
    40381 acgaactgat ctctcatttc caactgcgtg acgaagtgcc gtggtggaat gtggtaggcg
    40441 atcgtaaata ccagaaacag aaactggcgg gcgatcttga aaccctgcgc agctactatc
    40501 tggaccgcgg ttatgcccgt ttcaacatcg actctaccca ggtcagtctg acgccagata
    40561 aaaaaggtat ttacgtcacg gtgaacatca ccgaaggcga tcagtacaag ctttctggcg
    40621 ttgaagtgag cggcaacctt gccgggcact ccgctgaaat tgagcaactg actaagatcg
    40681 agccgggcga gctgtataac ggcactaaag tgaccaaaat ggaagatgac attaaaaagc
    40741 ttctcggtcg ctatggttat gcctatccgc gcgtacagtc gatgcctgaa attaacgatg
    40801 ccgacaaaac cgttaaatta cgcgtgaacg ttgatgcggg taaccgtttc tacgtgcgta
    40861 agatccgttt tgaaggtaac gatacctcga aagatgccgt cctgcgtcgc gaaatgcgtc
    40921 agatggaagg tgcatggttg gggagcgatc tggtcgatca gggtaaggag cgtctgaatc
    40981 gtctgggctt ctttgaaact gtcgataccg atacccaacg tgttccgggt agcccggacc
    41041 aggttgatgt agtctacaag gtgaaagaac gtaacactgg tagcttcaac ttcgggattg
    41101 gttacggtac tgaaagtggc gtgagcttcc aggctggcgt gcaacaggat aactggttag
    41161 gtacaggtta tgctgttggt atcaacggga ccaaaaacga ttaccagacc tatgctgaac
    41221 tgtcggtaac caacccgtac ttcaccgtag atggcgtaag cctcggtggt cgtctcttct
    41281 ataatgactt ccaggcagat gacgccgacc tgtccgacta taccaacaag agttatggta
    41341 cagacgtgac gttgggcttc ccgattaacg aatataactc gctgcgtgca ggtctgggtt
    41401 atgtacataa ctccctgtcc aacatgcagc ctcaggttgc gatgtggcgt tatctgtact
    41461 ctatgggtga acatccgagc acctctgatc aggataacag cttcaaaacg gacgacttca
    41521 cgttcaacta tggttggacc tataacaagc ttgaccgtgg ttacttcccg acagatggtt
    41581 cacgtgtcaa cctgaccggt aaagtgacca ttcctggatc ggataacgaa tactacaaag
    41641 tgacgttaga cacggcgact tatgtgccga tcgatgacga tcacaaatgg gttgttctgg
    41701 gtcgtacccg ctggggttat ggtgatggtt taggcggcaa agagatgccg ttctacgaga
    41761 acttctatgc cggtggttcc agcaccgtgc gtggcttcca gtccaatacc attggtccga
    41821 aagcagttta cttcccgcat caggccagta attatgatcc ggactatgat tacgaatgtg
    41881 cgactcagga cggcgcgaaa gacctgtgta aatcggatga tgctgtaggc ggtaacgcca
    41941 tggcggttgc cagcctcgag ttcatcaccc cgacgccgtt tattagcgat aagtatgcta
    42001 actcggttcg tacttcattc ttctgggata tgggtaccgt ttgggataca aactgggatt
    42061 ccagccaata ttctggatat ccggactata gtgatccaag caatatccgt atgtctgcgg
    42121 gtatcgcatt acaatggatg tccccattgg ggccgttggt gttctcctac gcccagccgt
    42181 tcaaaaagta cgatggagac aaggcagaac agttccagtt taacatcggt aaaacctggt
    42241 aagtgttctc cacaaaggaa tgtagtggta gtgtagcgat gactttaggc gatcaatata
    42301 agatcgccgg gccacgcaaa gaactgcacc ctccggtgca aatgggatgg taaggagttt
    42361 attgtgaaaa agtggttatt agctgcaggt cttggtttag cactggcaac ttctgctcag
    42421 gcggctgaca aaattgcaat cgtcaatatg ggcagcctgt tccagcaggt agcgcagaaa
    42481 accggtgttt ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga actgcagcgt
    42541 atggaaaccg atctgcaggc taaaatgaaa aaactgcagt ccatgaaagc gggcagcgat
    42601 cgcaccaaac tggaaaaaga cgtgatggct cagcgccaga cttttgctca gaaagcgcag
    42661 gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact ggttactcgt
    42721 atccagactg ctgtgaaatc cgttgccaac agccaggata tcgatctggt tgttgatgca
    42781 aacgccgttg cttacaacag cagcgatgta aaagacatca ctgccgacgt actgaaacag
    42841 gttaaataag taatgccttc aattcgactg gctgatttag cgcagcagtt ggatgcagaa
    42901 ctacacggtg atggcgatat cgtcatcacc ggcgttgcgt ccatgcaatc tgcacaaaca
    42961 ggtcacatta cgttcatggt taacccaaaa taccgtgagc atttaggctt gtgccaggcg
    43021 tccgcggttg tcatgaccca ggacgatctt cctttcgcga aaagtgccgc gctggtagtg
    43081 aagaatccct acctgactta cgcgcgcatg gcgcaaattt tagataccac gccgcagccc
    43141 gcgcagaaca ttgcacccag tgcggtgatc gacgcgacgg cgaagctggg taacaacgta
    43201 tcgattggcg ctaacgcggt gattgagtcc ggcgttgaac tgggcgataa cgtgattatc
    43261 ggtgccggtt gcttcgtagg taaaaacagc aaaatcggtg caggttcgcg tctctgggcg
    43321 aacgtaacca tttaccatga gatccagatc ggtcagaatt gcctgatcca gtccggaaca
    43381 gtggtaggcg cagacggctt tggttatgcc aacgatcgtg gtaactgggt gaagatccca
    43441 cagattggtc gcgtaattat tggcgatcgc gtggagatcg gtgcctgcac aaccatcgat
    43501 cgcggcgcgc tggatgacac tgttattggc aatggcgtga tcattgataa ccagtgccag
    43561 attgcacata acgtcgtgat tggcgacaat acggcggttg ccggtggcgt cattatggcg
    43621 ggcagcctga aaattggtcg ttactgcatg atcggcggag ccagcgtaat caacgggcat
    43681 atggaaatat gcgacaaagt gacggttacg ggcatgggta tggtgatgcg tcccatcact
    43741 gaaccaggcg tctattcctc aggcattccg ctgcaaccca acaaagtctg gcgcaaaacc
    43801 gctgcactgg tgatgaacat tgatgacatg agcaagcgtc tgaaatcgct tgagcgcaag
    43861 gttaatcaac aagactaacg ttccatcttt tgttcgccaa actttacggc ctgtctcatt
    43921 cttacgattg cggcaggccg tgttattatt gtcgtttctt atattttgac aggaagagta
    43981 tcttgactac taacactcat actctgcaga ttgaagagat tttagaactt ctgccgcacc
    44041 gtttcccgtt cttactggtg gatcgcgtgc tggattttga agaaggtcgt tttctgcgcg
    44101 cagtaaaaaa tgtctctgtc aatgagccat tcttccaggg ccatttccct ggaaaaccga
    44161 ttttcccggg tgtgctgatt ctggaagcaa tggcacaggc aacaggtatt ctggcgttta
    44221 aaagcgtagg aaaactggaa ccgggtgagc tgtactactt cgctggtatt gacgaagcgc
    44281 gcttcaagcg cccggtcgtg cctggcgatc aaatgatcat ggaagtcact ttcgaaaaaa
    44341 cgcgccgcgg cctgacccgt tttaaagggg ttgctctggt cgatggtaaa gtagtttgcg
    44401 aagcaacgat gatgtgtgct cgtagccggg aggcctgata cgtgattgat aaatccgcct
    44461 ttgtgcatcc aaccgccatt gtggaagagg gcgcgtcaat tggcgcgaac gcacacattg
    44521 gtcctttttg tatcgttgga ccccatgtcg aaattggtga gggtaccgta ctgaaatctc
    44581 acgttgtcgt gaatggtcat actaaaattg gccgcgataa tgagatttat cagtttgcct
    44641 ccatcggcga agttaaccag gatttgaaat atgctggcga accgacccgt gtggaaatcg
    44701 gcgatcgtaa ccgcattcgc gaaagcgtca ccattcatcg tggcacagtc cagggcggtg
    44761 gattgacgaa ggtgggcagc gacaacttac tgatgatcaa cgcgcacatt gcgcacgatt
    44821 gtacggtagg taaccgctgt attctcgcca acaacgcaac gctggcgggt cacgtatcgg
    44881 ttgacgactt cgcgatcatc ggcggcatga ccgcagtcca tcagttctgc atcattggtg
    44941 cgcatgtgat ggttggcggc tgctccggtg tggcgcagga cgtccctcct tatgttattg
    45001 cgcagggtaa ccacgcaacg ccgttcggtg tcaatatcga agggctgaag cgccgcggat
    45061 tcagccgtga ggcgattacc gctatccgca atgcgtataa gctaatttat cgtagcggta
    45121 aaacgctcga tgaagtgaaa ccggaaattg ctgaactggc ggaaacatat ccggaagtga
    45181 aagcctttac cgatttcttt gcacgctcaa cgcgcggtct gattcgttaa tgactgacca
    45241 gcgtccatta acgattgccc tggtcgccgg agaaacctcc ggcgatatcc tgggggccgg
    45301 tttaatccgc gctctgaaag aacgtgtgcc caacgcccgc tttgttggtg ttgccgggcc
    45361 acgaatgcag gctgaaggct gcgaagcctg gtacgaaatg gaagaactgg cggtgatggg
    45421 cattgttgaa gtgctcggtc gtctgcgtcg cttactgcat attcgtgccg atctgacaaa
    45481 gcgttttggc gaactgaagc cagatgtttt tgttggtatt gatgcgcccg acttcaatat
    45541 tactcttgaa ggtaacctca aaaagcaggg tatcaaaacc attcattacg tcagtccatc
    45601 cgtctgggcg tggcgacaga aacgcgtttt caaaataggc agagccaccg atctggtgct
    45661 cgcatttctg cctttcgaaa aagcgtttta tgacaaatac aatgtaccgt gccgctttat
    45721 cggtcatacc atggctgatg ccatgccgtt agatccagat aaaaatggtg cccgtgatgt
    45781 gctggggatc ccttacgatg cccactgtct ggcattgttg ccgggcagcc gtggtgcaga
    45841 agtcgaaatg cttagtgccg atttcttgaa aactgcccag cttttgcgcc agacatatcc
    45901 ggatctcgaa atcgtggtgc cgctggtgaa tgccaaacgc cgcgagcagt ttgaacgcat
    45961 caaagctgaa gtcgcgccag acctgtcggt tcatttgctg gatggaatgg gccgtgaggc
    46021 gatggtcgcc agcgatgcgg cactactggc gtcggggacg gcagccctgg agtgtatgct
    46081 ggcgaaatgc ccgatggtgg tgggatatcg catgaagcct tttaccttct ggttggcgaa
    46141 gcggctggtg aaaactgatt atgtctcgct gccaaatctg ctggcgggca gagagttagt
    46201 caaagagtta ttgcaggaag agtgtgagcc gcaaaaactg gctgcggcgc tgttaccgct
    46261 gttggcgaac gggaaaacca gccacgcgat gcacgatacc ttccgtgaac tgcatcagca
    46321 gatccgctgc aatgccgatg agcaggcggc acaagccgtt ctggagttag cacaatgatc
    46381 gaatttgttt atccgcacac gcagctggtt gcgggtgtgg atgaagtcgg acgcgggccg
    46441 ttagttggcg cggtcgtcac cgctgcggtg atccttgacc cggcgcgccc gattgccggg
    46501 ctgaatgatt ccaaaaagct gagcgaaaaa cgccgtctgg cgctctatga agagatcaaa
    46561 gagaaagcgt tgagctggag tctgggccgc gcggaacccc acgaaatcga cgagctgaac
    46621 attcttcatg cgaccatgct ggcgatgcag cgtgccgtcg ctgggctgca tattgcgccg
    46681 gaatatgtgt tgattgatgg taaccgctgc ccgaaattac cgatgcctgc gatggctgtg
    46741 gtgaaagggg atagccgcgt accagaaatc agtgccgcgt ctatcctggc gaaagtgacg
    46801 cgtgacgccg aaatggcggc gctggatatt gttttcccgc aatatggttt tgcccaacat
    46861 aaagggtatc caaccgcttt tcatctggaa aaactggctg aatatggcgc gactgaacat
    46921 catcgtcgca gctttgggcc tgtcaaacgc gcactgggac ttgcgtcctg attcttgtgt
    46981 cgagattaag taaaccggaa tctgaagatg tctgaaccac gtttcgtaca cctgcgggtg
    47041 cacagcgact actcgatgat cgatggcttg gccaaaaccg caccgctggt aaaaaaggcg
    47101 gcggcgttgg gtatgccagc actggcgatc accgatttca ccaacctttg cggtctggtg
    47161 aagttctacg gagcgggaca tggcgcaggg attaagccta tcgtcggggc agattttaat
    47221 gtccagtgcg acctgctggg tgatgagtta acccacctga cggtactggc ggcgaacaat
    47281 accggctatc agaatctggc gttgctgatc tcaaaagcgt atcagcgcgg gtacggtgcc
    47341 gccgggccga tcatcgatcg cgactggctt atcgaattaa acgaagggtt gatccttctt
    47401 tccggcgggc gcatgggcga cgtcggacgc agtcttttgc gtggtaacag cgcgctggta
    47461 gatgagtgtg tcgcgtttta tgaagaacac ttcccggatc gctattttct cgagctgatc
    47521 cgcaccggca ggccggatga agaaagctat ctgcacgcgg cggtggaact ggcggaagcg
    47581 cgcggtttgc ccgtcgtggc gactaacgat gtgcgcttta tcgacagcag cgactttgac
    47641 gcacacgaaa tccgcgtcgc gatccacgac ggctttaccc tcgacgatcc taaacgcccg
    47701 cgtaactatt cgccgcagca atatatgcgt agcgaagagg agatgtgtga gctgtttgcc
    47761 gacatcccag aagcccttgc caacaccgtt gagatcgcca aacgctgtaa cgtaactgtg
    47821 cgtcttggtg aatacttcct gccgcagttc ccgaccgggg acatgagcac cgaagattat
    47881 ctggtcaagc gtgcaaaaga aggcctggaa gagcgtctgg catttttatt ccctgacgag
    47941 gaagaacgtc ttaaacgccg cccggaatat gacgaacgtc tggagactga acttcaggtt
    48001 atcaaccaga tgggcttccc gggctacttc ctcatcgtta tggaatttat ccagtggtcg
    48061 aaagataacg gcgtaccggt agggccaggc cgtggctccg gtgcgggttc actggtggcc
    48121 tacgcgctga aaatcaccga cctcgatccg ctggaatttg acctgctgtt cgaacgtttc
    48181 cttaacccgg aacgtgtctc catgcctgac ttcgacgttg acttctgtat ggagaaacgc
    48241 gatcaggtta tcgaacacgt ggcggacatg tacggtcgtg atgcggtatc gcagattatc
    48301 accttcggta cgatggcggc gaaagcggtg atccgcgacg taggccgcgt gctggggcat
    48361 ccgtacggct ttgtcgaccg tatctcgaaa ctgatcccgc ccgatccggg gatgacgctg
    48421 gcgaaagcgt ttgaagccga gccgcagctg ccggaaatct acgaagcgga tgaagaagtt
    48481 aaggcgctga tcgacatggc gcgcaaactg gaaggggtca cccgtaacgc cggtaagcac
    48541 gccggtgggg tggttatcgc gccgaccaaa attaccgatt ttgcgccgct ttattgcgat
    48601 gaagagggca aacatccggt cacccagttt gataaaagcg acgttgaata cgccggactg
    48661 gtgaagttcg acttccttgg tttgcgtacg ctcaccatca tcaactgggc gctggagatg
    48721 atcaacaagc ggcgggcgaa gaatggcgag ccaccgctgg atatcgccgc gatcccgctg
    48781 gatgacaaga aaagcttcga catgctgcaa cgctcggaaa ccacggcggt attccagctt
    48841 gaatcgcgcg gcatgaagga cctgatcaag cgtctgcaac ctgactgctt cgaagatatg
    48901 atcgcactgg tggcactgtt ccgccctggt ccgttgcagt cagggatggt ggataacttt
    48961 atcgaccgta aacatggtcg tgaagagatc tcctatccgg acgtgcagtg gcagcatgaa
    49021 agcctgaaat cggtactgga gccaacctac ggcattatct tgtatcagga acaggtcatg
    49081 cagattgccc aggtgctttc tggttatacc ctcggtggcg cggatatgct gcgccgtgcg
    49141 atgggtaaga aaaagccgga agagatggct aagcagcgtt ctgtatttgc tgaaggtgca
    49201 gaaaagaacg gaatcaacgc cgaactggcg atgaaaatct tcgacctggt ggagaaattc
    49261 gcgggttacg gatttaacaa atcgcactct gcggcctatg ctttggtgtc atatcaaacg
    49321 ttatggctga aagcgcacta tccggcggag tttatggcgg cggtaatgac cgccgatatg
    49381 gacaacaccg agaaggtggt gggcctggtg gatgagtgct ggcggatggg gctgaaaatc
    49441 ctgccaccag atataaactc cggtctttac catttccacg tcaacgacga tggcgaaatc
    49501 gtgtatggta ttggcgccat caaaggggta ggtgaaggtc cgattgaggc catcatcgaa
    49561 gcccgtaata aaggcggcta cttccgcgaa ctgtttgatc tctgcgcccg taccgacacc
    49621 aaaaagttaa accggcgggt gctggaaaaa ctgatcatgt ccggggcgtt tgaccgtctt
    49681 gggccacacc gcgcggcgct gatgaactcg ctgggtgatg cgttaaaagc ggcagatcaa
    49741 cacgcgaaag cggaagctat cggtcaggcc gatatgttcg gcgtgctggc cgaagagccg
    49801 gaacaaattg aacaatccta cgccagctgc caaccgtggc cggagcaggt ggtattagat
    49861 ggggaacgtg aaacgttagg cctgtacctg accggacacc ctatcaacca gtatttaaaa
    49921 gagattgagc gttatgtcgg aggcgtaagg ctgaaagaca tgcacccgac agaacgtggt
    49981 aaagtcatca cggctgcggg gctcgttgtt gccgcgcggg ttatggtcac caagcgcggc
    50041 aatcgtatcg gtatctgcac gctggatgac cgttccgggc ggctggaagt gatgttgttt
    50101 actgacgccc tggataaata ccagcaattg ctggaaaaag accgcatact tatcgtcagc
    50161 ggacaggtca gctttgatga cttcagcggt gggcttaaaa tgaccgctcg cgaagtgatg
    50221 gatattgacg aagcccggga aaaatatgct cgcgggcttg ctatctcgct gacggacagg
    50281 caaattgatg accagctttt aaaccgactc cgtcagtctc tggaacccca ccgctctggg
    50341 acaattccag tacatctcta ctatcagagg gcggatgcac gcgcgcggtt gcgttttggc
    50401 gcgacgtggc gtgtctctcc gagcgatcgt ttattaaacg atctccgtgg cctcattggt
    50461 tcggagcagg tggaactgga gtttgactaa tacaggaata ctatgagtct gaatttcctt
    50521 gattttgaac agccgattgc agagctggaa gcgaaaatcg attctctgac tgcggttagc
    50581 cgtcaggatg agaaactgga tattaacatc gatgaagaag tgcatcgtct gcgtgaaaaa
    50641 agcgtagaac tgacgcgtaa aatcttcgcc gatctcggtg catggcagat tgcgcaactg
    50701 gcacgccatc cgcagcgtcc ttataccctg gattacgttc gcctggcatt tgatgaattt
    50761 gacgaactgg ctggcgaccg tgcgtatgca gacgataaag ctatcgtcgg tggtatcgcc
    50821 cgtctcgatg gtcgtccggt gatgatcatt ggtcatcaaa aaggtcgtga aaccaaagaa
    50881 aaaattcgcc gtaactttgg tatgccagcg ccagaaggtt accgcaaagc actgcgtctg
    50941 atgcaaatgg ctgaacgctt taagatgcct atcatcacct ttatcgacac cccgggggct
    51001 tatcctggcg tgggcgcaga agagcgtggt cagtctgaag ccattgcacg caacctgcgt
    51061 gaaatgtctc gccttggcgt accggtagtt tgtacggtta tcggtgaagg tggttctggc
    51121 ggtgcgctgg cgattggcgt gggcgataaa gtgaatatgc tgcaatacag cacctattcc
    51181 gttatctcgc cggaaggttg tgcgtccatt ctgtggaaga gcgccgacaa agcgccgctg
    51241 gcggctgaag cgatgggtat catcgctccg cgtctgaaag agctgaaact gatcgactcc
    51301 atcatcccgg aaccgctggg cggcgctcac cgtaacccgg aagcgatggc ggcatcgttg
    51361 aaagcgcaac tgctggcgga tctggccgat ctcgatgtgt taagcactga agatttaaaa
    51421 aatcgtcgtt atcagcgcct gatgagctac ggttacgcgt aattcgcaaa agttctgaaa
    51481 aagggtcact tcggtggccc ttttttatcg ccacggtttg agcaggctat gattaaggaa
    51541 ggattttcca ggaggaacac atgaacatca ttgccattat gggaccgcat ggcgtctttt
    51601 ataaagatga gcccatcaaa gaactggagt cggcgctggt ggcgcaaggc tttcagatta
    51661 tctggccaca aaacagcgtt gatttgctga aatttatcga acataaccct cgaatttgcg
    51721 gcgtgatttt tgactgggat gagtacagtc tcgatttatg cagcgatatc aatcaactta
    51781 atgaatatct cccgctttat gccttcatca acacccactc gacgatggat gtcagcgtgc
    51841 aggatatgcg gatggcgctc tggttttttg aatatgcgct ggggcaggcg gaagatatcg
    51901 ccattcgtat gcgtcagtac accaacgaat atcttgataa cattacgccg ccgttcacga
    51961 aagccttgtt tacctacgtc aaagagcgga agtacacctt ttgtacgccg gggcatatgg
    52021 gcggcaccgc atatcaaaaa agcccggttg gctgtctgtt ttatgatttt ttcggcggga
    52081 atacccttaa ggctgatgtc tctatttcgg tcaccgagct tggttcgttg cttgaccaca
    52141 ccgggccaca cctggaagcg gaagagtaca tcgcgcggac ttttggcgcg gaacagagtt
    52201 atatcgttac caacggaata tcgacgtcga acaaaattgt gggtatgtac gccgcgccat
    52261 ccggcagtac gctgttgatc gaccgcaact gccataaatc actggcgcat ctgttgatga
    52321 tgaacgatgt agtgccagtc tggctgaaac cgacgcgtaa tgcgttgggg attctggggg
    52381 ggatcccgcg ccgtgaattt actcgcgaca gcatcgaaga gaaagtcgct gccactacgc
    52441 aagcacaatg gccggttcat gcggtgatca ccaactccac ctatgatggt ttgctctaca
    52501 acaccgactg gatcaaacag acgctggatg tcccgtcgat ccacttcgat tccgcctggg
    52561 tgccgtatac tcattttcat ccaatctacc agggtaaaag tggtatgagc ggcgagcgtg
    52621 ttgcgggaaa agttttcttc gaaacgcaat cgacccacaa aatgctggcg gcgttatcgc
    52681 aggcttcact gatccacatt aaaggcgagt atgacgaaga ggcgtttaac gaagccttta
    52741 tgatgcatac caccacctcg cccagttatc ccattgttgc ttcggttaag acggcggcgg
    52801 cgatgctgcg tggtaatccg ggcaaacggc tgattaaccg ttcggtggag cgggcgctgc
    52861 attttcgcaa agaggtccag cggctgcggg aagagtctga cggttggttt ttcgatatct
    52921 ggcaaccgcc gcaggtggat gaagccgaat gcaggcccgt tgcgcctggc gaacaatggc
    52981 acggctttaa cgatgcggat gccgatcata tgtttctcga tccggttaaa gtcactattt
    53041 tgacaccggg gatggacgaa cagggcaata tgagtgagga ggggatcccg gcggcgctgg
    53101 tagcaaaatt cctcgacgaa cgtgggatcg tagtagagaa aaccggccct tataacctgc
    53161 tgtttctctt tagtattggc atcgataaaa ccaaagcaat gggattattg cgtgggttga
    53221 cggaatttaa gcgctcttac gatctcaacc tgcggatcaa aaatatgctg cccgatctct
    53281 atgctgaaga tcccgatttc taccgcaata tgcgtattca ggatctggcg caagggatcc
    53341 ataagctgat tcgtaaacac gatctttccg gtttgatgtt gcgggcattc gatactttgc
    53401 cggaaatgat catgacgcca catcaggcat ggcagagaca gattaaaggt gaagtagaaa
    53461 ccattgcgct ggaacaactg gtcggtagag tatcggcaaa tatgatcctg ccttatccac
    53521 cgggcgtacc gctgttgatg ccaggagaaa tgctgaccaa agagagccgc acggtactcg
    53581 attttctact gatgctttgt tccgtagggc aacattaccc cggttttgaa acggatattc
    53641 acggcgcgaa acaggacgaa gacggcgttt accgcgtacg agtcctaaaa atggcgggat
    53701 aacttgccag agcggcttcc gggcgagtaa cgtgctgtta acaaataaag gagacgttat
    53761 gctgggttta aaacaggttc accatattgc gattattgcg acggattatg cggtgagcaa
    53821 agctttctac tgcgatattc ttggtttcac gctgcaaagc gaagtctatc gcgaagcgcg
    53881 cgactcgtgg aaaggggatt tggcgcttaa tgggcaatat gtgattgagc ttttctcttt
    53941 tccgttcccg ccggagcgcc ccagccgacc ggaagcttgc ggcctgcgtc atctggcttt
    54001 tagcgttgat gacatcgatg cggcagtggc gcaccttgaa agccataacg tgaagtgtga
    54061 agccatccgt gtcgatccat acacgcaaaa acgcttcacc ttctttaacg atccggacgg
    54121 gctgccgttg gaactgtatg agcagtaagg cttgtcatcg ccgcatttgc ccggtaacgt
    54181 gccgggcatt gctactgtaa aatcgcacca tcatgacact cacgctcaat agacaacttc
    54241 tcacctcacg ccagattctg gtggccttta gcggcgggct tgactccacc gttctgctgc
    54301 atcagttggt gcagtggcgg acggaaaatc cgggcgtcac tctgcgcgct attcatgtgc
    54361 atcacggttt aagtgccaat gccgatgcct gggttacgca ttgcgaaaac gtctgccaac
    54421 agtggcaggt gccgctggtg gtcgaacgcg tacaacttgc gcaagagggg ctggggattg
    54481 aggcccaggc gcggcaggca cgttatcagg catttgcccg taccctattg cccggcgaag
    54541 tgctggtcac cgcgcaacat ctcgacgatc aatgtgaaac ctttctgctg gcgctaaaac
    54601 gcggtagcgg ccctgccggg ctttcggcta tggcggaagt ttcggagttt gctggaacac
    54661 ggcttatccg cccgttgctt gcccgcacgc ggggggaact ggagcagtgg gcattggcgc
    54721 atggtttacg ctggattgaa gacgaaagta atcaggacga ctcatacgat cgtaactttc
    54781 tgcgcctgcg cgtagtgccg ttattgcagc agcgttggcc gcattttgcg gaagcaacgg
    54841 cccgcagcgc cacactttgt gctgaacaag agagtctgtt ggatgaactg ctggcggatg
    54901 atttagcaca ctgccaaacg tcgcagggga cgctgcagat tgcgccaatg ctggcgatga
    54961 gtgatgcccg tcgcgcggcg attattcgcc gctggctggc agggcagaat gcaccgatgc
    55021 cttcccgcga cgcgctggtg cgcatctggc aggaagtggc gctggcgcgg gaagatgctt
    55081 ccccctgttt acgtttgggc gcctttgaaa tccgccgcta tcagtcgcaa ctgtggtgga
    55141 ttaaatccgt caccgggcaa agcgaaacca ttgtgctgtg gcagacgtgg ctacaaccgc
    55201 tggaattacc ggcggggctg ggaaccgtac agcttactgc gggaggcgat attcgccctc
    55261 cgcgtgcaga cgaagcggtc agcgtgcgtt tcaaagcgcc aggattgctg catattgtcg
    55321 ggcgcaacgg cggacgtaag ctgaagaaaa tctggcaaga gttaggcgtg ccgccgtggc
    55381 tacgtgacac cacgccactg ctgttttatg gagaaacgct gattgcggcg gcaggggtat
    55441 ttgtgacgca agaaggtgtg gctgaaggtg agaatggcgt aagttttgtc tggcagaaaa
    55501 cgcttagtta agtgaaagcc ggataagacg catcaaacgt cgcatccggc gaaagtcaat
    55561 caggattcgc ttaccaccac cgtaccgatt tcagggtggc taaagctggt aattttatcc
    55621 agacgcagct cgcgggtttc gccagcggcc tcgacgacca gatactccac atttttgcgg
    55681 gagactaaat cactggcttt tgcctgcaat ttttcgccat ctttcagctc aagtgtcagc
    55741 attaaatgat gctggcaggc gagctcaaga ttatcgtaat catcacaatt aattggttga
    55801 tacgtatcat tcattgacat aatcgctcac cagtaagttt gccgcagcgt atgctgcttt
    55861 ttccctgaca gcctcagaaa gggcgtcgtc ggcagccatt tcattcagca ctttcaaaac
    55921 gcagcccagc gcgtccggaa cgtatcctaa gtctccgctg gcgatttccg cgtaccgctt
    55981 gcgtattaac tcacaatatt tttccacatg ccctcctgtc agcactctga cttaaccgtg
    56041 gatgcaagtc taagcctacg aagataaact ctgtttcgca aggtgactat accacactca
    56101 tttctgcaat atcagcgccg caactgcacg tattccgtta caatggcctc ctgattcgaa
    56161 aggagttttc ttatggcgct taaagcgaca atttataaag cgacggttaa tgtggccgat
    56221 ctcgaccgca accagtttct cgatgcctct ctgacgctgg cgcgccaccc ttcagaaacc
    56281 caggagcgta tgatgctgcg cttgctggcg tggctgaaat atgctgatga acgtctgcaa
    56341 tttacccgtg gtttgtgtgc cgatgatgag ccggaagcgt ggctgcgtaa cgatcacctg
    56401 ggcattgatt tgtggattga gctggggctg ccggatgagc gacgaattaa gaaagcctgc
    56461 acccaggccg cagaagtggc gctgtttacc tataatagtc gggcggcgca aatctggtgg
    56521 cagcaaaatc agagcaaatg tgtgcagttt gccaatcttt ccgtctggta tctggacgat
    56581 gaacaactgg cgaaagtaag cgcctttgcc gatcgtacca tgacgctgca ggcaacgatt
    56641 caggatggtg ttatctggtt atcggatgat aagaataatc tggaagttaa cttaaccgcc
    56701 tggcaacaac cttcatgatt gtgatttccc gacatgttgc tattcccgat ggtgagcttg
    56761 agatcaccgc cattcgtgcg cagggcgctg gcgggcagca tgttaataag acctcaacgg
    56821 ctattcatct gcgttttgac attcgggcgt ccagcctgcc agagtattac aaagagcgtc
    56881 tgctcgccgc cagccatcat ttgatcagca gtgatggcgt gattgtcatt aaggcacagg
    56941 aataccgcag tcaggaactg aaccgcgaag ccgctctggc ccggctggtg gcagtgatta
    57001 aagatttaac aaaagaacaa aaagcccgac gacccacgcg gcccacccgt gcatcgaaag
    57061 agcgcaggct ggcatcgaaa gcacaaaaat caagcgtgaa ggcgatgcgc ggcaaagtac
    57121 gcagcggtcg ggaataaaaa gaaggaatgg atggtgaaaa aagcgatagt gacagcgatg
    57181 gctgtaatca gcctctttac tctgatggga tgtaataatc gggccgaagt cgatacgctt
    57241 tctccggcgc aggctgccga actgaaaccg atgccgcaaa gttggcgcgg cgtgctaccg
    57301 tgtgccgatt gcgaaggaat cgaaatctct ctgttcctcg aaaaagacgg aacatgggtg
    57361 atgaatgagc gttatctcgg tgctcgtgaa gaaccttcct ccttcgcttc ctacggtaca
    57421 tgggcgcgaa ccgctgacaa gctggtatta accgacagca aaggtgaaaa gtcatattat
    57481 cgggcgaaag gcgatgcgct ggagatgctc gatcgtgaag gcaatccaat tgaatcgcag
    57541 ttcaactata cgctggaacc ggcacaatcc agtttaccta tgacgccgat gaccctgcga
    57601 ggcatgtatt tttatatggc tgatgcggcg accttcactg attgcgcgac cggaaaacgt
    57661 ttcatggtag cgaataacgc agagctggag cgtagctacc tggctgcgcg cggtcacagt
    57721 gaaaaaccgg tgttactgtc agtagaaggt cactttacgc ttgaggctaa tccggatact
    57781 ggtgcgccaa ccaaagtatt agcgcccgat acggcgggta aattttaccc gaataaggat
    57841 tgcagtagtt tggggcagta aacctgctta aggcagaaac aaacgcaaaa ttgcctgatg
    57901 cgctgcgctt atcaggccta cagcatataa tgcaacattt tgaattagca cgattttgta
    57961 ggccggataa ggcgtttacg ccgcatccgg cattttgtgc acgaggctgg ctttatcact
    58021 gagtcagccc aacaaagcga cccgccttga tataaatccc aggcttcagg tgcccaacat
    58081 actgtaactg cgtttctggc ttaaacgcag agacatcacc aatacgcata tgcatcagat
    58141 cggcagggct aatccagccc gattgcgcca gtgtaagtgg atgcccggat ttggcaaatg
    58201 catcagtgac aaattccgaa caaaaccacg actttttgtc tccttcgcac acactgctta
    58261 actgcgcttt cgccaggtcg ctgacgcact gttggcgaaa atcctcggag aacggattca
    58321 gtgagcacat ctggcgagtc accataaagg gaataaattc gacaatgccg cgatagttat
    58381 aaccgctatc tttgatttta ttggcaaacg cggtgatatc tgtggcttgt tgcggggtaa
    58441 gatccgggac tcgtaagacg aaaagcttat cactatgctt catcgctttt ttaagggaaa
    58501 caatctggac gccagcgcct gtcgcttctg caacgttatt atcacccaga aagattgcaa
    58561 cgtgactcac agaggaagtg ctgaagacgc ggattccaaa tgaggttacc ccaaggcttg
    58621 aggagaacag caaatcgccg ggtttgagat caggtgccgt tatttctttt attgattgtt
    58681 cggtgaaaga gctttgatgc tggaatttga cagcccatgt ttttgcctcg gcatctactg
    58741 ctgtggctga tgggtctggc tggctgatat caacggtaca agccgaaagt aaaagaaagc
    58801 tggggagaag caggcggcag tacgcctttg gtttatccat tttatacaat ccatgtaaaa
    58861 aagggccctg aaattcagga ccctttctgg catcagcctt taatctgttt caccagatat
    58921 tcgacgatgt caccagtctt aattaactgt ttctcgccgt tgcgacgata tttatattcg
    58981 atatcgtcgt tgtcgaggtt acggtcgccc agcacaatag tgtgcggaat accgatcagt
    59041 tccatatcag caaacatcac gcccggacgc tctttgcggt catccagcag cacttcgata
    59101 ccttgtgcac gcagttcgct gtacagtttc tcagcaagct cttgtacgcg gaaggatttg
    59161 tgcatgttca tcggcagaat cgccacctgg aacggcgcga tagcgtcagg ccatacgatg
    59221 ccgcgttcgt cgtagttctg ctcaatcgcc gcagctacca cacgcgttac cccgataccg
    59281 tagcaaccca tcgtcaggat ttggttacgg ccatcttcac cctgtacgga ggctttcagt
    59341 gcttcggagt acttggtacc cagctggaag atgtgaccaa cttcgatacc acgtttgatc
    59401 agcagcgtac cctggccatc cgggcttgga tcgccagcca ccacgttacg gatatctgca
    59461 acttccgggg tagcgacatc gcgatcccag ttgataccga agtagtgttt accatcgatg
    59521 ttagcaccag cagcgaaatc actcatcgcc gcaacggtac ggtcaatcac caccggaatc
    59581 ggcatgttta ccggacccag tgaacccgga ccggctttaa ccacagcacg aatttcttct
    59641 tcggtcgcga aagtcagcgg gcttgcaacc tgcggcagtt tttctgcttt aacttcgttc
    59701 agctcgtgat caccgcgcac cagcagcgca accagcggga agctactgcc ttcaaccgct
    59761 ttaaccagca gagtcttaac cgttttctca atcggcagat tgaactgttc aaccagttcc
    59821 gcgatggttt tcgcgttcgg cgtatcaacc agcgtcattt cctgggtagc agcagcgcgc
    59881 ggttctttcg gcgcgatagc ttctgccagt tcaatgttcg ctgcatagtc agaggtgtcg
    59941 gagaagacca catcgtcttc accgctctgc gccagcacct ggaattcgtg agaggcgctg
    60001 ccgccgatag aaccggtgtc ggcctgtacg gcgcggaaat ccagccccat gcggctgaag
    60061 attttgctgt aggccgcata cattgcatcg taggtttcct gcagggattc ctgagaagta
    60121 tggaaagagt aagcatcttt catcaggaat tcgcgggaac gcatgacgcc gaaacgcgga
    60181 cgcacttcgt cgcggaactt ggtctggatc tgatagaagt tcagcggcag ctgtttgtaa
    60241 gagctaagct cgttacgaat caggtcagtg ataacttctt catgagttgg gccgagtacg
    60301 aacggacgct cgccacggtc aacaaaacgc agcagttccg gaccgtactg ttcccaacga
    60361 ccactctctt gccacaaatc ggctggctga accaccggca tcgacacctc gatcgcaccg
    60421 gcgttgttca tctcttcacg cacgatgttt tcgacttttt tcagaacgcg cacgccggtc
    60481 ggcagccagg tatataaccc ggaggccagc ttgcggatca tcccggcgcg cagcatcagc
    60541 tgatggctga tcacctcggc gtcggcaggt gtctccttga gagtggagag caggtattgg
    60601 ctagtacgca tgttgttacg gttccagttg gaaggtagaa caggctcaag gcgagcctgg
    60661 gacaaaaaaa gtgatttagt ttaccagtgc aaaagaaatg tcaaaagaga agggcgtgaa
    60721 tttaacgcgg ttccagcgca aagacttcaa aacctgcgtc ggtgacgcgc cagcgaacgt
    60781 taaaatcatg tagccagacg gcataggttt tgcccgtttc ctcaccttta cgataggccg
    60841 ggcgcgggtc ctgcgccagt acttcgcgga taaacagcgt taactgcgga taacgcttct
    60901 ccagcgtcaa aagctgcttt tcgacctctg cggtaaaact caccgccatc tctgcagctg
    60961 gcgcactttg cgcatagctg gcactggcat cgggaagcga ttcggcaaag gggagatacg
    61021 gtttgatatc cactaccggc gtaccatcga ccagatccag actgccgagc ttcagaatca
    61081 cgctgccttt atggcaaaca acctctttca gctctaccag cgacatgcca attgggttag
    61141 ggcggaaagt agagcgtgtt gcgaaaaccc ccattctggc gttaccgccg aggcgcggtg
    61201 gacgcacagt cggacgccag ccgccttcca tcgtttgatg aaagacgaaa aggatccata
    61261 aatggctgaa tgcttccagg ccgcgaacgg cgtcggcctg gttgtaggaa gctatgagat
    61321 gcagttcgcc gttggcgctt tttaccagac ctggctggcg cggaacggcg aatttttctt
    61381 tatagggcga gcgaataacg cctatttgct cgaactggaa actgctcatt tcgccgtaat
    61441 gttaagcgca gagccgatac atacagcctg acgatagcag cccggcgtac cgctggtgac
    61501 ttcgcagcta tgcagtaata cagcattggc tttcatttta gaggcattga tttgcatctg
    61561 cttacgtgcg gttggaatgc tcggcggaga gtcctgatta gaggcctggc aagagtcgcc
    61621 actgacttca ccgagatcgc ggaacggttt gccgactaat tcttctgcat tggtataaat
    61681 tcggaccggc gtggcgcgcg gcgctttcgg ttttgcaggc tccgctttcg gctggggtgc
    61741 agtgctttga acgggttcga caggggatct gcttaacatg gaacagccgc ttagcatgag
    61801 tgctactaaa cagatcggta aagcacgcat agtatttcct caatgtatga tcaaaacgtc
    61861 aatattgaat caggagcttg taaaaatgac aagacgggca agcgcccgtc ctgaatgata
    61921 ttacaaattg tggaaacagc ctaaaaatta ccagccttta acagcgccgc cgttaaacac
    61981 tttgtttgct gcttcgtaaa cttcatcaga ctgataagcc tggacgaatt tcttcacgtt
    62041 ctcggcgtct ttgttatctt cacgcgttac gatcaggttt acgtatgggg agtctttatc
    62101 ttcgacaaag ataccgtctt tcgccggagt caggccaatc tggctggcat aggtggtatt
    62161 gataactgcc agagcgattt gcgcgtcgtc cagagaacgc ggcagttgcg gtgcttccag
    62221 ttcaacaatt ttcagatttt ttgggttctc aacaacatca agaacggtcg gcagcaggcc
    62281 aacgccatct ttcagtttga tcaagcccac tttttgcagc agcagcagtg aacgaccaag
    62341 attagttgga tcgtttggca cggcaacctg cgaaccatcc tgcagttcat ccagtgattt
    62401 gattttcttg gagtaaccag caatcggata aacaaaagtg ttgcctactg cgaccagttt
    62461 gtagccacga tctttcagtt gctgatcaag gtacggttta tgctggaagg cgttggcgtc
    62521 gatatcgcct ttgctcaatg cttcgtttgg cagaacatag tcgttgaagg ttaccagctc
    62581 aacgtccagg ccatatttgt ctttcgcaac tttctgcgcg acttctgcta cctgctgttc
    62641 ggcaccaaca atcacgccga ctttaatgtg gtttggatct ttttcatcct gaccgcagcc
    62701 taccagtgcc agtgatccaa tcagggctcc cactgccgca aaggttttga atttgaacgc
    62761 cataccttat tccttcttca attatttatg ttgtgttgaa cgttacttgc gagtgacagc
    62821 ccggacgatg cggtcgcctg caaactgaat taaataaacc agaatgacca gcaataccag
    62881 taccgtattc atcaccgtcg cgttgtagcc gatgtagcca tactgatagc caatctgacc
    62941 taaaccaccg gcaccgactg caccacccat cgcggaataa ccgaccaggg taatcagggt
    63001 gatagttgcc gcattcacca gacccggcaa cgcttccggt aacagcactt tacagacgat
    63061 ctgcattggc gtggcaccca ttgcgcggga agcttcaatt aacccggttg ggatctccag
    63121 cagagcgttc tcgaccatac gggcaataaa cggtgctgca ccaacggtta acggaacaat
    63181 tgctgcctgc aaaccaatcg atgtaccgac aataacgcgg gtaaacggaa tcatccatac
    63241 cagcaagata atgaacggga tggaacggaa aatgttcaca atcgcagaaa cggtacgata
    63301 cagcttcgcg ttagcaataa tttgccccgg acgcgtgaca taaagcagaa cgccaaccgg
    63361 cagaccaatc acaaacccaa aaaatccgga tacgaaggtc attgccagcg tttcccatac
    63421 gccacgaacc agcagccaca tcatcggctc agacataacc cagtacctct acttttacat
    63481 ggtgttcctg cagccaggca atggcggctt gcgtatcttg ttgtgtgccg tgcatttcag
    63541 tcagcatgat gccgaattta acgccaccgg cgtagtccat ctgcgcgcta ataatgttgt
    63601 tgttgacgtt gaaacgacgc gcggtttcag aaagcagtgg ggcatcgacc gattgaccgg
    63661 taaactccag acgcagcatc ggcacgcagt cagtaaatgg ctccgtttgc agacgttcct
    63721 ggtaatcttc cgggatatcc agatgcaggg tcgactgaat aaacttctgc gccagcggcg
    63781 ttttcggatg cgagaacact tcacttaccg tgtcctgctc gatcagttcg ccattgctga
    63841 tgaccgccac gcaatcacaa atacgcttca caacgtccat ttcgtgggtg atcaacagaa
    63901 tcgtcaaacc cagacggcgg ttaatgtctt tcagcagttc gagaatagaa cgtgtcgttg
    63961 ccgggtccag cgcgctggtg gcttcatcac acagcaatac tttgggattg ctggctaatg
    64021 cacgggcaat cgccacacgt tgtttctgcc caccggaaag attcgacggg tagctatcat
    64081 gcttatcgcc aagaccaacc aatgacagca attccgtcac gcgacgtttg atctcgtctt
    64141 tcggtgtgtt gtccagctcc agcggcagag ccacgttgcc aaaaacagta cgcgaagaga
    64201 gcaggttaaa atgctggaaa atcataccaa tctggcggcg agctttggtc aactcggatt
    64261 ctgacagcgt ggtcaattcc tggccatcga ccagcacgct accctcggtt gggcgctcca
    64321 gcaagtttac acaacgtata agcgtactct tacccgcgcc tgaggcaccg ataacgccat
    64381 aaatttgtcc agctggcaca tgcaggctga cgttgttcaa cgcctggatg gtgcgggtgc
    64441 cctggtggaa cactttggtg atattcgaaa gttttatcat tgattattta ttatcgtcat
    64501 taagttagtc gtagcatctc gaatgcctga aacgggcaac gccgtcaatg aaatggatgt
    64561 taaggcatcc agacgtctaa atcaatcagg tttatgcgaa gagcactttc ttgcaggtcg
    64621 aaacatgcga tactagcgtc ccatgcctta ttaaggagct ataaaaggtg gcgaagagcg
    64681 tacccgcaat ttttcttgac cgtgatggca ccattaatgt cgatcacggc tatgtccatg
    64741 agatcgacaa ctttgaattt atcgacggtg ttattgacgc catgcgcgag ctaaaaaaaa
    64801 tgggctttgc gctggtggta gtaaccaacc agtctggcat tgctcgcggt aaatttaccg
    64861 aagcacagtt tgaaacgctg accgagtgga tggactggtc gctggcggac cgagatgtcg
    64921 atctggatgg tatctattat tgcccgcatc atccgcaggg gagtgttgaa gagtttcgcc
    64981 aggtctgcga ttgccgcaaa ccacatccgg ggatgctttt gtcagcacgc gattatttgc
    65041 atattgatat ggccgcttct tatatggtgg gcgataaatt agaagatatg caggcagcgg
    65101 ttgcggcgaa cgtgggaaca aaagtgctgg tgcgtacggg taaacctatt acacctgaag
    65161 cagaaaacgc ggcagattgg gtgttaaata gcctggcaga cctgccgcaa gcgataaaaa
    65221 agcagcaaaa accggcgcaa tgattaaaag atgaacggtt gaaataaaaa tgcatttttc
    65281 cgcttgtctt cctgagccga ctccctataa tgcgcctcca tcgacacggc ggatgtgaat
    65341 cacttcacac aaacagccgg ttcggttgaa gagaaaaaat cctgaaattc agggttgact
    65401 ctgaaatagg aaagtgtgat atacgccacc tcgcgacagt gagctgaaag ctgcgtcgca
    65461 actgctcttt aacaatttat cagacaatct gtgtgggcac tcgaagatac ggattcttaa
    65521 cgtcgcaaga cga
//
LOCUS       DAZOYF010000006        60419 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZOYF010000006 DAZOYF010000000
VERSION     DAZOYF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.006"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..322
                     /locus_tag="ACHUOF_000372"
     CDS             <1..322
                     /locus_tag="ACHUOF_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0609715.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="ACHUOF_000373"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="ACHUOF_000373"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR0609716.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="ACHUOF_000374"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="ACHUOF_000374"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR0609717.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHUOF_000375"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHUOF_000375"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR0609718.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="ACHUOF_000376"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="ACHUOF_000376"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR0609719.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="ACHUOF_000377"
     CDS             complement(3726..4955)
                     /locus_tag="ACHUOF_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR0609720.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="ACHUOF_000378"
     CDS             complement(5019..5459)
                     /locus_tag="ACHUOF_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR0609721.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="ACHUOF_000379"
     CDS             complement(5564..6334)
                     /locus_tag="ACHUOF_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0609722.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="ACHUOF_000380"
     CDS             complement(6331..7257)
                     /locus_tag="ACHUOF_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0609723.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="ACHUOF_000381"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="ACHUOF_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR0609724.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHUOF_000382"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHUOF_000382"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR0609725.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="ACHUOF_000383"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="ACHUOF_000383"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR0609726.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHUOF_000384"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHUOF_000384"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR0609727.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMVIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="ACHUOF_000385"
     CDS             12964..13311
                     /locus_tag="ACHUOF_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR0609728.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="ACHUOF_000386"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="ACHUOF_000386"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR0609729.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="ACHUOF_000387"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="ACHUOF_000387"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR0609730.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHUOF_000388"
     CDS             complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHUOF_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR0609731.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHUOF_000389"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHUOF_000389"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR0609732.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="ACHUOF_000390"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="ACHUOF_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACHUOF_000391"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACHUOF_000391"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR0609733.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACHUOF_000392"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACHUOF_000392"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR0609734.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACHUOF_000393"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACHUOF_000393"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR0609735.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACHUOF_000394"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACHUOF_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR0609736.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="ACHUOF_000395"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="ACHUOF_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR0609737.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACHUOF_000396"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACHUOF_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR0609738.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACHUOF_000397"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACHUOF_000397"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR0609739.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="ACHUOF_000398"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="ACHUOF_000398"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR0609740.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="ACHUOF_000399"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="ACHUOF_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR0609741.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /locus_tag="ACHUOF_000400"
                     /pseudo
     CDS             32769..33916
                     /locus_tag="ACHUOF_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="ACHUOF_000401"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="ACHUOF_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR0609742.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACHUOF_000402"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACHUOF_000402"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR0609743.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="ACHUOF_000403"
                     /pseudo
     CDS             complement(36348..36386)
                     /locus_tag="ACHUOF_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="ACHUOF_000404"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="ACHUOF_000404"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR0609744.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="ACHUOF_000405"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="ACHUOF_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR0609745.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="ACHUOF_000406"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="ACHUOF_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR0609746.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACHUOF_000407"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACHUOF_000407"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR0609747.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACHUOF_000408"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACHUOF_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR0609748.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACHUOF_000409"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACHUOF_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR0609749.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNPNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACHUOF_000410"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACHUOF_000410"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR0609750.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACHUOF_000411"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACHUOF_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR0609751.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACHUOF_000412"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACHUOF_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR0609752.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACHUOF_000413"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACHUOF_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR0609753.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACHUOF_000414"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACHUOF_000414"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0609754.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACHUOF_000415"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACHUOF_000415"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR0609755.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACHUOF_000416"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACHUOF_000416"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR0609756.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACHUOF_000417"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACHUOF_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR0609757.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACHUOF_000418"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACHUOF_000418"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR0609758.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACHUOF_000419"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACHUOF_000419"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR0609759.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACHUOF_000420"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACHUOF_000420"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR0609760.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACHUOF_000421"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACHUOF_000421"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR0609761.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACHUOF_000422"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACHUOF_000422"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR0609762.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACHUOF_000423"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACHUOF_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR0609763.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACHUOF_000424"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACHUOF_000424"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR0609764.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACHUOF_000425"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACHUOF_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR0609765.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14473 a        16732 c        14848 g        14366 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctaccca aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 caccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgtctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcaac aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gccccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcgg attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DAZOYF010000007        55511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZOYF010000007 DAZOYF010000000
VERSION     DAZOYF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.007"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            88..1574
                     /locus_tag="ACHUOF_000426"
                     /pseudo
     CDS             88..1574
                     /locus_tag="ACHUOF_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
     gene            1589..2323
                     /gene="ypdB"
                     /locus_tag="ACHUOF_000427"
     CDS             1589..2323
                     /gene="ypdB"
                     /locus_tag="ACHUOF_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR0609766.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2336..3193
                     /locus_tag="ACHUOF_000428"
                     /pseudo
     CDS             2336..3193
                     /locus_tag="ACHUOF_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3196..5698)
                     /gene="ptsP"
                     /locus_tag="ACHUOF_000429"
                     /pseudo
     CDS             complement(3196..5698)
                     /gene="ptsP"
                     /locus_tag="ACHUOF_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5723..6767)
                     /gene="ypdE"
                     /locus_tag="ACHUOF_000430"
                     /pseudo
     CDS             complement(5723..6767)
                     /gene="ypdE"
                     /locus_tag="ACHUOF_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(6767..7183)
                     /locus_tag="ACHUOF_000431"
     CDS             complement(6767..7183)
                     /locus_tag="ACHUOF_000431"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFR0609767.1"
                     /translation="MTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQ
                     QIDDAARRVITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIY
                     LLGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(7180..7287)
                     /locus_tag="ACHUOF_000432"
     CDS             complement(7180..7287)
                     /locus_tag="ACHUOF_000432"
                     /inference="COORDINATES: protein motif:HMM:NF019360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609768.1"
                     /translation="MARGAAARQSQRQDCCSGRVCHSRFWCAVSGLLLI"
     gene            complement(7248..7850)
                     /gene="ypdF"
                     /locus_tag="ACHUOF_000433"
                     /pseudo
     CDS             complement(7248..7850)
                     /gene="ypdF"
                     /locus_tag="ACHUOF_000433"
                     /inference="COORDINATES: protein motif:HMM:NF007310.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7865..9112)
                     /locus_tag="ACHUOF_000434"
     CDS             complement(7865..9112)
                     /locus_tag="ACHUOF_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR0609769.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9134..9460)
                     /locus_tag="ACHUOF_000435"
     CDS             complement(9134..9460)
                     /locus_tag="ACHUOF_000435"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR0609770.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9679..10644)
                     /gene="glk"
                     /locus_tag="ACHUOF_000436"
     CDS             complement(9679..10644)
                     /gene="glk"
                     /locus_tag="ACHUOF_000436"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR0609771.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            10848..12104
                     /locus_tag="ACHUOF_000437"
     CDS             10848..12104
                     /locus_tag="ACHUOF_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR0609772.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12219..12545
                     /gene="ypeC"
                     /locus_tag="ACHUOF_000438"
     CDS             12219..12545
                     /gene="ypeC"
                     /locus_tag="ACHUOF_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR0609773.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12686..13924)
                     /locus_tag="ACHUOF_000439"
     CDS             complement(12686..13924)
                     /locus_tag="ACHUOF_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR0609774.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14260..15462
                     /gene="nupC"
                     /locus_tag="ACHUOF_000440"
     CDS             14260..15462
                     /gene="nupC"
                     /locus_tag="ACHUOF_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR0609775.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15512..17624)
                     /locus_tag="ACHUOF_000441"
                     /pseudo
     CDS             complement(15512..17624)
                     /locus_tag="ACHUOF_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(17832..17907)
                     /locus_tag="ACHUOF_000442"
     tRNA            complement(17832..17907)
                     /locus_tag="ACHUOF_000442"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17872..17874),aa:Ala,seq:ggc)
     gene            complement(17947..18022)
                     /locus_tag="ACHUOF_000443"
     tRNA            complement(17947..18022)
                     /locus_tag="ACHUOF_000443"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17987..17989),aa:Ala,seq:ggc)
     gene            18243..18602
                     /locus_tag="ACHUOF_000444"
     CDS             18243..18602
                     /locus_tag="ACHUOF_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0609776.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18604..18996
                     /locus_tag="ACHUOF_000445"
     CDS             18604..18996
                     /locus_tag="ACHUOF_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0609777.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19048..20463)
                     /gene="gltX"
                     /locus_tag="ACHUOF_000446"
     CDS             complement(19048..20463)
                     /gene="gltX"
                     /locus_tag="ACHUOF_000446"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR0609778.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20722..20797
                     /locus_tag="ACHUOF_000447"
     tRNA            20722..20797
                     /locus_tag="ACHUOF_000447"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20755..20757,aa:Val,seq:tac)
     gene            20842..20917
                     /locus_tag="ACHUOF_000448"
     tRNA            20842..20917
                     /locus_tag="ACHUOF_000448"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20875..20877,aa:Val,seq:tac)
     gene            20922..20997
                     /locus_tag="ACHUOF_000449"
     tRNA            20922..20997
                     /locus_tag="ACHUOF_000449"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20955..20957,aa:Lys,seq:ttt)
     gene            21028..21153
                     /locus_tag="ACHUOF_000450"
     ncRNA           21028..21153
                     /ncRNA_class="other"
                     /locus_tag="ACHUOF_000450"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            21273..21614
                     /locus_tag="ACHUOF_000451"
     CDS             21273..21614
                     /locus_tag="ACHUOF_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR0609779.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(21605..22531)
                     /locus_tag="ACHUOF_000452"
     CDS             complement(21605..22531)
                     /locus_tag="ACHUOF_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0609780.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            22621..23619
                     /locus_tag="ACHUOF_000453"
     CDS             22621..23619
                     /locus_tag="ACHUOF_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR0609781.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23616..23834)
                     /locus_tag="ACHUOF_000454"
     CDS             complement(23616..23834)
                     /locus_tag="ACHUOF_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR0609782.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23836..25851)
                     /gene="ligA"
                     /locus_tag="ACHUOF_000455"
     CDS             complement(23836..25851)
                     /gene="ligA"
                     /locus_tag="ACHUOF_000455"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR0609783.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25922..26908)
                     /gene="zipA"
                     /locus_tag="ACHUOF_000456"
     CDS             complement(25922..26908)
                     /gene="zipA"
                     /locus_tag="ACHUOF_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR0609784.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            27138..27899
                     /gene="cysZ"
                     /locus_tag="ACHUOF_000457"
     CDS             27138..27899
                     /gene="cysZ"
                     /locus_tag="ACHUOF_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR0609785.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            28084..29055
                     /gene="cysK"
                     /locus_tag="ACHUOF_000458"
     CDS             28084..29055
                     /gene="cysK"
                     /locus_tag="ACHUOF_000458"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR0609786.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29439..29696
                     /gene="ptsH"
                     /locus_tag="ACHUOF_000459"
     CDS             29439..29696
                     /gene="ptsH"
                     /locus_tag="ACHUOF_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR0609787.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29741..31468
                     /gene="ptsI"
                     /locus_tag="ACHUOF_000460"
     CDS             29741..31468
                     /gene="ptsI"
                     /locus_tag="ACHUOF_000460"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR0609788.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31509..32018
                     /gene="crr"
                     /locus_tag="ACHUOF_000461"
     CDS             31509..32018
                     /gene="crr"
                     /locus_tag="ACHUOF_000461"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR0609789.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(32060..32911)
                     /gene="pdxK"
                     /locus_tag="ACHUOF_000462"
                     /gene_synonym="thiJ"
     CDS             complement(32060..32911)
                     /gene="pdxK"
                     /locus_tag="ACHUOF_000462"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR0609790.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            33016..33390
                     /locus_tag="ACHUOF_000463"
     CDS             33016..33390
                     /locus_tag="ACHUOF_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR0609791.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            33423..34146
                     /locus_tag="ACHUOF_000464"
                     /pseudo
     CDS             33423..34146
                     /locus_tag="ACHUOF_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(34350..35261)
                     /gene="cysM"
                     /locus_tag="ACHUOF_000465"
     CDS             complement(34350..35261)
                     /gene="cysM"
                     /locus_tag="ACHUOF_000465"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR0609792.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(35395..36492)
                     /gene="cysA"
                     /locus_tag="ACHUOF_000466"
     CDS             complement(35395..36492)
                     /gene="cysA"
                     /locus_tag="ACHUOF_000466"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR0609793.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(36482..37357)
                     /gene="cysW"
                     /locus_tag="ACHUOF_000467"
     CDS             complement(36482..37357)
                     /gene="cysW"
                     /locus_tag="ACHUOF_000467"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR0609794.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(37357..38190)
                     /gene="cysT"
                     /locus_tag="ACHUOF_000468"
     CDS             complement(37357..38190)
                     /gene="cysT"
                     /locus_tag="ACHUOF_000468"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR0609795.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(38190..39206)
                     /gene="cysP"
                     /locus_tag="ACHUOF_000469"
     CDS             complement(38190..39206)
                     /gene="cysP"
                     /locus_tag="ACHUOF_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR0609796.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(39510..40301)
                     /gene="ucpA"
                     /locus_tag="ACHUOF_000470"
     CDS             complement(39510..40301)
                     /gene="ucpA"
                     /locus_tag="ACHUOF_000470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR0609797.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(40430..41288)
                     /gene="murR"
                     /locus_tag="ACHUOF_000471"
                     /pseudo
     CDS             complement(40430..41288)
                     /gene="murR"
                     /locus_tag="ACHUOF_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
     gene            41452..42348
                     /gene="murQ"
                     /locus_tag="ACHUOF_000472"
     CDS             41452..42348
                     /gene="murQ"
                     /locus_tag="ACHUOF_000472"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR0609798.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            42352..43776
                     /gene="murP"
                     /locus_tag="ACHUOF_000473"
     CDS             42352..43776
                     /gene="murP"
                     /locus_tag="ACHUOF_000473"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFR0609799.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            43781..43867
                     /locus_tag="ACHUOF_000474"
                     /pseudo
     CDS             43781..43867
                     /locus_tag="ACHUOF_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(43956..44855)
                     /gene="yfeX"
                     /locus_tag="ACHUOF_000475"
     CDS             complement(43956..44855)
                     /gene="yfeX"
                     /locus_tag="ACHUOF_000475"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR0609800.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(44951..45526)
                     /locus_tag="ACHUOF_000476"
     CDS             complement(44951..45526)
                     /locus_tag="ACHUOF_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR0609801.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(45587..46036)
                     /locus_tag="ACHUOF_000477"
     CDS             complement(45587..46036)
                     /locus_tag="ACHUOF_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR0609802.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(46023..46448)
                     /locus_tag="ACHUOF_000478"
     CDS             complement(46023..46448)
                     /locus_tag="ACHUOF_000478"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR0609803.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            46662..47531
                     /gene="amiA"
                     /locus_tag="ACHUOF_000479"
     CDS             46662..47531
                     /gene="amiA"
                     /locus_tag="ACHUOF_000479"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR0609804.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            47535..48434
                     /gene="hemF"
                     /locus_tag="ACHUOF_000480"
     CDS             47535..48434
                     /gene="hemF"
                     /locus_tag="ACHUOF_000480"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR0609805.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(48440..49492)
                     /gene="eutR"
                     /locus_tag="ACHUOF_000481"
                     /pseudo
     CDS             complement(48440..49492)
                     /gene="eutR"
                     /locus_tag="ACHUOF_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(49538..50038)
                     /gene="eutK"
                     /locus_tag="ACHUOF_000482"
     CDS             complement(49538..50038)
                     /gene="eutK"
                     /locus_tag="ACHUOF_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR0609806.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(50051..50710)
                     /gene="eutL"
                     /locus_tag="ACHUOF_000483"
     CDS             complement(50051..50710)
                     /gene="eutL"
                     /locus_tag="ACHUOF_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR0609807.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(50720..50788)
                     /locus_tag="ACHUOF_000484"
                     /pseudo
     CDS             complement(50720..50788)
                     /locus_tag="ACHUOF_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            51892..52683
                     /locus_tag="ACHUOF_000485"
     CDS             51892..52683
                     /locus_tag="ACHUOF_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609808.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            53403..53798
                     /locus_tag="ACHUOF_000486"
     CDS             53403..53798
                     /locus_tag="ACHUOF_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR0609809.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            53816..54079
                     /locus_tag="ACHUOF_000487"
     CDS             53816..54079
                     /locus_tag="ACHUOF_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609810.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            54102..54644
                     /locus_tag="ACHUOF_000488"
     CDS             54102..54644
                     /locus_tag="ACHUOF_000488"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609811.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            54783..55070
                     /locus_tag="ACHUOF_000489"
     CDS             54783..55070
                     /locus_tag="ACHUOF_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609812.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            55109..55315
                     /locus_tag="ACHUOF_000490"
     CDS             55109..55315
                     /locus_tag="ACHUOF_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0609813.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
BASE COUNT        13849 a        14728 c        13728 g        13206 t
ORIGIN      
        1 tttatgtttt ttcctcaaaa acggcctgct ggtcatcagc cttcaggtta cctatcatag
       61 aggtttaatc cttattcaga gtcacccgtg cacgaaatat tcaacatgct gctggcggtc
      121 ttcgatcggg cagcgttaat gcttatctgc ctgttctttc tcatccgtat ccgcctgttt
      181 cgcgaactgt tgcacaagtc ggcgcactcc ccaaaagaat tgctcgccgt taccgccatt
      241 ttctcgctgt tcgccctgtt cagcacctgg tccggcgttc ccgtagaagg ctcgctggtg
      301 aacgtacgta ttatcgcggt gatgtccggc gggattctgt ttggcccgtg ggtaggcatc
      361 attaccggcg tgattgcggg tattcaccgg tatttaattg atatcggcgg cgtgacggcg
      421 atcccctgct ttatcaccag cattctggcg ggttgtatat caggctggat aaacctgaaa
      481 atccccaaag cacagcgctg gcgctggata tcgccaacaa aacgctaccg ctgtttcgcc
      541 atgtcaatag cgagtcatta cgcaaggtct gcgaaattat ccgcgatgac attcacgccg
      601 atgcggtggc gattactaat accgatcatg tgctggccta tgttggcgtg ggtgaacata
      661 actatcagaa tggcgatgac ttcattagcc cgactacccg tcaggcgatg aattacggaa
      721 aaatcatcat taaaaacaat gatgaagccc accgcacacc agagattcat tccatgctgg
      781 tgatcccatt gtgggagaaa ggggtcgtga ccggaacgct gaaaatttac tactgccacg
      841 cgcatcagat cacctcgtca ttacaggaaa tggcggtcgg tctgtcgcaa atcatctcca
      901 cgcaactgga ggtttcacgc gccgagcagc tacgtgaaat ggcaaataag gcagagattc
      961 gcgccctgca aagcaaaatt aatccccatt ttctgtttaa cgctctgaac gctatttcat
     1021 cgtcaatccg tctgaatccg gataccgctc gccagttgat ctttaatctg tcgcgttatc
     1081 tgcgctataa cattgaatta aaagacgatg agcaaatcga tatcaaaaaa gagctgtatc
     1141 aaattaaaga ctatattgcc attgagcagg cccgctttgg cgacaagctg acggttatct
     1201 atgatattga tgaagaggtg aattgctgca ttcccagcct gctgatccag ccgttggtgg
     1261 agaacgccat tgtccacggt attcagcctt gcaaaggtaa aggcgttgtc accatcagcg
     1321 ttgcagagtg cggaaatcgg gtacgcattg cggtgcgaga taccgggcac ggcatcgttc
     1381 caaaggtgat tgagcgggtc gaagcgaatg aaatgccggg caataaaatt ggcctgctga
     1441 atgtccatca tcgcgtgaag ttattgtatg gcgaggggct gcatatccgc cgcctggagc
     1501 cggggacgga aattgcgttt tacattccta accaacgcac cccagtcgcc tcacaggcta
     1561 cgttattgct ttgagccgga gtgatattgt gaaagtcatc attgttgaag acgaattcct
     1621 ggcacaacag gaactgagct ggctaattaa agagcacagc cagatggaga ttgtcggcac
     1681 ctttgacgac ggtctggacg tgttgaagtt tttgcagcat aaccgcgtcg acgccatttt
     1741 tctggatatc aatattccgt cgctggatgg cgtgttgctg gcgcaaaaca tcagccagtt
     1801 cgcccataaa ccgtttattg tgttcatcac cgcgtggaaa gaacatgcgg tagaagcgtt
     1861 tgaactggag gcgtttgact acattctcaa accgtatcag gagtcacgta ttaccgggat
     1921 gctgcaaaaa ctggaagcgg cctggcaaca acagcagacc agcagtacgc ctgccgcgac
     1981 ggtaacgcgt gagaatgaca ccattaatct ggtgaaagat gagcgaataa tcgtcacgcc
     2041 aattaacgat atctattacg ccgaagcgca cgagaaaatg acctttgtct atacgcggcg
     2101 tgaatcctac gtaatgccga tgaacattac cgaattttgc agcaaactgc cgccgtcgca
     2161 ttttttccgc tgccatcgct cattttgtgt caatctgaac aaaatacgcg aaatcgaacc
     2221 gtggtttaat aacacctaca ttctgcgact gaaagatctg gattttgaag tgccggtcag
     2281 ccgcagcaaa gtgaaagaat ttcgccagtt aatgcatctt taatcgggaa ctttcatgaa
     2341 agcaccaggc ttgcccgccg atcaacaatt tttcgccgat ctgttcagcg gcctagtgct
     2401 taacccgcaa ctactggggc gcgtctggtt tgccagccag cctgcctcgt taccggtggg
     2461 cagtttatgt attgattttc cccgtctgga tatcgtgctg cgcggcgaat acggcaacct
     2521 gctggaagca aagcagcagc gaatggtgga aggagaaatg ctgtttattc cggcacgcgc
     2581 ggctaattta ccgatcaaca acaaaccggt gatgctgtta agcctggtgt tcgcaccgac
     2641 ctggcttggg ttatcgtttt acgatagccg caccacgtcg ttgttgcatc ctgctcgcca
     2701 gacccagctt cccagcctgc aacgcggtga aggtgaagcg atgcttaccg ccctcaccca
     2761 tcttagccgt tcgccgctgg agcagaatat cattcagcca ctggtgttaa gtttgctgca
     2821 tctttgccgc aacgtggtga atatgccgcc gggtaattca caaccgcgcg gcgattttct
     2881 ctatcacagc atttgtaact gggtttagga taattatgcc cagccgctca cccgcgagag
     2941 cgtggcgcag ttttttaata tcacgcccaa tcatctgtca aaactgtttg ctcagcatgg
     3001 aacgatgcgt tttatcgagt atgtgcgttg ggtgcgaatg gcgaaggcga ggatgatttt
     3061 gcagaaatat catctgtcaa ttcatgaagt ggcacagcgt tgcggttttc cggatagcga
     3121 ctatttttgt cgcgttttcc ggcgtcagtt tagtctgacg ccgggagagt acagcgcccg
     3181 ttttcagggc taacgtcaga aggttaattc tgtttccagc agcgtcagga tactttgtgc
     3241 atcttgcgcg gcaaacaacg actggcggaa gtttttattc accagtttac gcgccagctg
     3301 cgagaagact ttcacatgat tcatcccttc gttagcaccc agcgtcagca tgatcaccag
     3361 ttcgacttcg cccatttctg actgccagtc aaccggtttc gccagccggg caatgctgat
     3421 actggaatga cggatccact gagatttggt gtgcgggatc gctacgccaa aaccaacgcc
     3481 ggtggtaaca atctcttccc gctgccagac atcttcttcc agctcaaacg gatgttcagt
     3541 gcgcccgtta acgccgaggt tgccgcacag gaactggatc gcctgctctt tattgctaaa
     3601 atcctgatca acaaagatat tctccagcgc cagcagtggg cgaacgtctt cttccggcgt
     3661 aaaggcggtg agtaacgctt caatttcctg cgcactgcgg cattcacatg cctgacgcgc
     3721 cagttcccga cacgcctcgc tatccagttg acgaagctgg cttttcaccg ccggaatacg
     3781 cgggctactc atactcagct catccaggcc cagcccaagc agtagcggca gataacggct
     3841 ttcaccgccc agttcaccgc aaatgcctac ccatttgccc cgctgatgcg cagtggtaac
     3901 tatttgctgc aacatgcgca ggaacgatgg cgtaatcggg ttatatagcg gcgatacgcg
     3961 cgggttatta cgatcgaccg catacagata ctgggtcata tcgttggagc cgatactgaa
     4021 gaaatcgacc tcatcgcaga agtggtcgat gatgtagcac accgacggca cttccaccat
     4081 gatcccaagc gtaatcgtct ctgcatgacg caggccatcg cgcttaagct caacgatcgc
     4141 tttttggatc tcgcctttca cccataagat ctgatcgagg ctgtgaacca tcgggatcat
     4201 caactgggcg ttgccgaaac tggcggcgcg caaaatggcc cgcagttgag tgcggaacag
     4261 gccagcaaat tccgggtaaa tacgtaccgc gcgatagccg aggaacgggt tctcttcctg
     4321 gggaatattc agataaggaa tgcttttatc gccgccgatg tccatcgtgc ggaagataat
     4381 cggcttgtcg cccgccgccc gccgccagta gcacctgctg gtaggcttca aattgctcct
     4441 gctcgtccgg cgcgctgtcg cgatccatat agagcatttc ggtacggaac agcccgacac
     4501 cttccgcgcc gttggcaaac gcgcctggcg cttccagagc ggtgccgata ttcgccgcaa
     4561 tatcaatacg cttgttatca cgggaatagg ccagctgcgc ggcagcctgc gcctgttgtt
     4621 tttggcgttt atccgccagc gtctgcgcga cctgataata accgctcacc gcgtcattcg
     4681 ggttaatcgc cagcacgccg cactgggcgt caagcactgc aggttgcccg gcataacggg
     4741 caatcgcatc cagcggcaag ccactcagta ccgggatcgc cgaggcacgg gccagaatca
     4801 gtgtatgcga ggtgcgcccg gttttctcca gaatcatacc cgcaagattt ttcaaatcaa
     4861 ggctcaaaaa ctggcttggg gttaaatctt cagccaccag aatggtcggt ttttccagca
     4921 ccagattgtt gcgcggcttc agttccggcc aggtgatatg cagcaactgt tcgctgatat
     4981 cgcgaatgtc gctgacccgc tcgcgcagat aatcgctggc agaagcagat agtttggcgc
     5041 aaacctgctc catattgctg atgatcgccg cccccagtcc ctgatgctgt tctgtcatca
     5101 ggcgacggat attgcctgca aattcatcat cctgaatcag cgacaaatga gcgctgagga
     5161 tagttttgct ttcgccgtca cgctcacgca attgctgatt cagttgctcg gcaagcgttg
     5221 ccaggctgtg ctccagccgg gtggaatctt gcgcactggc ggggattgcc cgataactgt
     5281 cgaggctgtc gctttgtaac agggtcagcg tacccacgcc gacgccgctt gccagcacat
     5341 tgccgtacag taaatccggg ttaaggcggc ttaatgaacg cggcagcgga tgcgccgtca
     5401 gttctgcctg cgtcggctga acgctgtcgc tgtcgataaa gcgcaccagg atgtactctt
     5461 ccagcacgcg ccgcgcctgc tcttcatcgc tgccgctaat gttcaggctg caactgtcat
     5521 taaataaggt gccagttcca atcaacgcca gcgagctttt agcatcagcc tttgcgttct
     5581 ggcgatggtt gataaaggtg atttcgcttt gccactggct gcactgttct ttaagttccc
     5641 acgccggacg agcatgtaga ccgttaggca aaggacagag aaattgaatc gttaacataa
     5701 ccgctccggg ttaatcagga gatcatctga aatccgtcag ttgaacaacc gtctcacgcg
     5761 taagacgttg aataagggca gataaaagtt gctccatctg caaaatgtcg cggcaatcgg
     5821 caatcggcaa tcgatgccgc gcaatgtccg tggcgggtgg caggccccat taccaatgtg
     5881 ggtacgccag taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata
     5941 tccgcctgca acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt
     6001 ggcggcgcaa tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga
     6061 tggttagccg cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg
     6121 acgtccggcg acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg
     6181 gaacttgcca ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac
     6241 gtcaccagca gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga
     6301 gggaggacct gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc
     6361 atcacttcgt cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc
     6421 tgccggtcgc cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc
     6481 ggctgcagct ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct
     6541 tcgcgggaga tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc
     6601 ttcggacctg tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact
     6661 tctttttgca ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag
     6721 gcgatcgcat ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg
     6781 tgagcaacac tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca
     6841 caacatcttc gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca
     6901 gcaacatgcc tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga
     6961 cttcaatgcc gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg
     7021 taatgacccg gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag
     7081 agattgccgc gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt
     7141 cggcgctcac cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga
     7201 tacagcgcac caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct
     7261 ttgccgtgcg gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt
     7321 tctgcgccct gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc
     7381 cccgcctgaa taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg
     7441 cggattatct ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta
     7501 accagtttcg cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg
     7561 ccttcaaaac cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta
     7621 agcgtgttcg ttgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca
     7681 acgaaatagc gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct
     7741 gatccggtgg agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca
     7801 tccagttgtt gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc
     7861 cttcttacag gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca
     7921 ccaccatcag cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaagt
     7981 tagtgaccag cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc
     8041 agacagctgc ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg
     8101 ggctttccag cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg
     8161 cggtcttacc ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt
     8221 caataatggt cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg
     8281 tgaccggtaa tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga
     8341 tcgggccacc aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc
     8401 ccttctcacc tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg
     8461 gagtgatgac gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa
     8521 atgtggtttt gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca
     8581 caaggtaacc ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg
     8641 aagaggtcgc ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag
     8701 acatcaaccc gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca
     8761 caaatgcagc aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca
     8821 gccaggcaaa tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat
     8881 ccatgatgcc gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag
     8941 cgaggatcac accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat
     9001 gctgcgggag ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg
     9061 ccgcaccgga tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta
     9121 tccctgtcga atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc
     9181 ccgcagcgtt tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac
     9241 gttcgttatc ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat
     9301 cctgcgccgt caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat
     9361 aaccggcttc taccgccgct tcttccagca cctgagcggc cataaaagtg tgagccaggc
     9421 ccattgggca ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg
     9481 atagttgact agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg
     9541 catttactgg agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg
     9601 gacacaaaga aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc
     9661 cgatataaca ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga
     9721 accgagaaga cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc
     9781 tttaaagcgc cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag
     9841 gaagcgcggc acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc
     9901 cagattgccg ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc
     9961 ggtgcagctg tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag
    10021 gcggttgtca gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac
    10081 gcgctccgcc gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc
    10141 ttcactattc ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt
    10201 atcgacatgg accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg
    10261 cttaccttcg accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg
    10321 gatcgccatc gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt
    10381 ctttttcatt tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc
    10441 ggtaattggg caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag
    10501 ataaacgcga atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg
    10561 cgagatttca ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc
    10621 accgactaat gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt
    10681 ctcacactgt aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa
    10741 tatcgatctg ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt
    10801 tgcaagctgg gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc
    10861 gagccagaac catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc
    10921 ttattctgat tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac
    10981 tgccgggaac tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc
    11041 taacgggtat tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc
    11101 ccgcctgtga accgctgatc ggcgcaccgg ttccgccctc tgcgctacct agacttatcg
    11161 tagcattaat tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga
    11221 ccgtcaatat cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa
    11281 tggagtggac tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg
    11341 cggcggcgtt gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc
    11401 gtctttttgc gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc
    11461 atccccattt ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca
    11521 gcggtgcaat tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac
    11581 cacggttgca cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg
    11641 gatttattct cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg
    11701 atgagatgca gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg
    11761 cggtaattaa acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa
    11821 tcttcccggc agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg
    11881 ccgttccagc ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac
    11941 gcgatggctg gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac
    12001 cgctgctctg tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga
    12061 tggtcaatcg tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct
    12121 ttatgattta agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa
    12181 caggccgctg acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg
    12241 cggccgccct gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc
    12301 catcgatcaa attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc
    12361 actggcgaga tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc
    12421 gtcatgataa tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc
    12481 gtgaaggctg gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc
    12541 attaaacgcg tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg
    12601 cctacaaacc ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat
    12661 agtgccagat gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga
    12721 tattcagcgc cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt
    12781 tcaccagatc gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca
    12841 gggcgatacc aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc
    12901 ccatcagaat gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga
    12961 tatggaagcg aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg
    13021 aggacagtcc ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca
    13081 gcggttgcag cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt
    13141 ggaacgccgc cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg
    13201 cgatagccac atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat
    13261 gctgagtgag cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc
    13321 ctgctgccag gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca
    13381 gctgcgccag gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca
    13441 acagtaaccc gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa
    13501 tcaggaaagt cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga
    13561 gtttaaaacc gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt
    13621 ctgcctgaac ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg
    13681 ccagattttt accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca
    13741 tcaggttggc ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa
    13801 tattggtcgc aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca
    13861 ttaatgcgag cctcatctta cgcgccgccc gtccgctgct actctcaacg cgatagttcg
    13921 tcatcttgtg cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg
    13981 attatcaaat tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta
    14041 cgaaaagatt gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact
    14101 ttcagcttcc atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt
    14161 cacaggacgt cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat
    14221 gtgcacggaa atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt
    14281 gtactggcac ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa
    14341 attcgtatcc gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc
    14401 ctgaactccg acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg
    14461 ctcggatttg ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg
    14521 gcattcttct tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt
    14581 ctccagcaca ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa
    14641 gtcaacggta tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag
    14701 tcagaaaact ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac
    14761 accatggctg ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc
    14821 atgctggaac cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc
    14881 gtgctgtcgc tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc
    14941 aacctgcacg aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc
    15001 aaagttgcca ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg
    15061 aacgcactgt ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc
    15121 ctgggctaca tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg
    15181 caagtgggca gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat
    15241 ctgcagaaaa ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta
    15301 gtttccttcg ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat
    15361 gaagagcaag gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg
    15421 gtgagtgtgc tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc
    15481 cggggatcat tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa
    15541 tcaaataccc ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg
    15601 tctcgacaaa ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc
    15661 tcacaatcat cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt
    15721 tgatgatatc agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt
    15781 catcaatcgc aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt
    15841 ctgcgttaga aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt
    15901 gataacgctt aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg
    15961 gcattaaatt gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact
    16021 tcaacaagga ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa
    16081 ggggcagaaa cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct
    16141 catcataacc ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct
    16201 gatctaacgc cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa
    16261 ccatctcctc tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca
    16321 gccagcttaa ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc
    16381 aggcagcacc atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca
    16441 ggatgttaac catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca
    16501 gttcactacc cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac
    16561 ggcaaattga gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact
    16621 caagattatc aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt
    16681 ccaacgcacg aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc
    16741 ggcgattaag ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa
    16801 aggaaatcag caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa
    16861 agttctgatt gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg
    16921 gatagcgaag attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac
    16981 cggcaataaa gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg
    17041 ccagccagct taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact
    17101 gcggtgaaaa ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag
    17161 gtggatatct ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg
    17221 ccgatcggcg tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat
    17281 ccataacgcc aacgcgtccc cagctgccag cgtaatattg cgcaggcaca caacacgaca
    17341 aacgtctggc tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga
    17401 ttggtgcaaa acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag
    17461 attgacagca ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg
    17521 gcaagtggcg aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt
    17581 gtgaatatct tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca
    17641 ctgatcgggg ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg
    17701 gccagcctgt tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac
    17761 gtagactgta ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag
    17821 ggttggaaat ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa
    17881 gcgctctccc agctgagcta tagccccacg atgcgtttac gtaccaaatt tgctgggtgc
    17941 aaaatttggt ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct
    18001 ctcccagctg agctatagcc ccgtcacgta aagcttgtcg agttgacggg cggcatcata
    18061 tgaattccgc ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat cgctgaaaaa
    18121 tcaggcaaat gaacaatttt ggaaaaccgc tcgcattcag tagttattca tgtcacggtt
    18181 tcctgtaaag tggtgttata aaatgaacta ctaatagacc cacatacatt cagggaattg
    18241 ttatgttcaa ggagaggatg acaccagatg aacttgccag actgaccggc tatagccgcc
    18301 agaccattaa taaatgggta cgcaaggaag gctggacaac gtcaccaaaa cctggcgtcc
    18361 agggtggcaa agcccgactg gttcacgtca atgaacaggt tcgtgaatat attcgcaatg
    18421 ctgaacgtcc agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg ccgcttgaag
    18481 tattgctggt gacactggcg aaagagatga cgccagttga acaaaaacag tttacatcct
    18541 tgcttctgcg ggaagggatt atcggattgt tacaacgctt agggattcgc gatagcaaat
    18601 aatatgaaaa gattacgcaa taaaatgacc accgaagaac tggctgaatg cctcggtgtg
    18661 gctaaacaaa ctgttaaccg ttggaccaga gaaaaaggct ggaaaacgga aaaatttccc
    18721 ggcgtgaaag gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg cgagtttatt
    18781 cagaacacgc cagccttcca taacacacca atgttgatgg aagctgaaga gagaattgct
    18841 gaatacgctc ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc gatagataat
    18901 atgacagaca ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg aattcgcaat
    18961 ttcctcgccc gtctcgatat tgacgaatca gcataaataa aaaacggcag gatattatct
    19021 cctgccgttt atctttttac acgctaatta ctgctgattt tcgcgttcag caataaaatc
    19081 cagcgctttg ttgatacgct cgatactgcg ggtcttacca attgcgtgaa cggtaacatc
    19141 cagtgctgga gactgccccg caccggttac ggcgacacgc agcggcatac caactttacc
    19201 catacccact tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt tttcagcggt
    19261 ccagtcagta atcgcggcca gtttgtcacg aaccacttcc agcggctgac gcgctaccgg
    19321 acgcagatgt tttttcgccg catcggcatc gaactcagca aaatcttcgt agaaataacg
    19381 gcagctctgt gccatctctt tcagcgtctt gcagcgttcg cccagcagtt tcaccagatc
    19441 agccagctgc gggccgttac gggtatcgat attttcctgc tcaatgtgcc actgtaagtg
    19501 agtagcaaca tactccggcg gcagcgcgtt aatgtagtga tggttcagcc acagcagctt
    19561 gtcggtgttg aacgcactgg cagatttgct gacggcattc aaagtgaagt atttgatcat
    19621 ctcttcacga gtgaagattt cctgatcgcc gtgggaccag cccagacgca ccagatagtt
    19681 cagcagtgct tctggcaaat aaccgtcatc acgatactgc attacgctga ctgccccgtg
    19741 acgtttggac agttttttac cgtcatcgcc attgatcata gaaacgtgcg cgtaaaccgg
    19801 caccggcgct ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt tgatatggtc
    19861 ttcgccacgg ataacgtggg tgatttccat atcccagtca tcgacaacca cacagaagtt
    19921 ataggttggg gaaccatcgg tacggcggat aataagatcg tccagttcct ggttgctgaa
    19981 ctcgatcgga ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg ggttagcaaa
    20041 acgtacaaca cacggttcat catcagcatg atgctcatgg ctgtggcggc agcgaccgtc
    20101 ataacgcggc ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca ggcgctcttt
    20161 agagcaatag catttataag cagtgccctc ttccagcatc tgatcgatca ccgcgttgta
    20221 gcgatcaaaa cgtttggtct ggtagtacgg accttcatcc cactccaggc tcagccagtt
    20281 catgccatcc ataatggctt cgatagcttc cggcgtggaa cgctcaagat cggtgtcttc
    20341 aatacgcagc acgaactcac cgccgtggtt acgtgcaaaa agccaggagt aaagagcagt
    20401 acgcgcgccg ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag ttttgatttt
    20461 catgaaatgg ccttacgttt agaaagatgc cgacaaccgg caaatcctgg aaaaattgag
    20521 tgggcgatat tctatcactc ccgcctgatt cctcaatgta gttcgggttt ctaccttacg
    20581 cattattgtt ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca tcttgcttaa
    20641 atttacgacg aacgaacaat ttctttagaa aatgcgttga ctcattttga actctcccta
    20701 taatgcgact ccacacagcg ggggtgatta gctcagctgg gagagcacct cccttacaag
    20761 gagggggtcg gcggttcgat cccgtcatca cccaccaact actttatgta gtctccgccg
    20821 tgtagcaaga aattgagaag tgggtgatta gctcagctgg gagagcacct cccttacaag
    20881 gagggggtcg gcggttcgat cccgtcatca cccaccactt cgggtcgtta gctcagttgg
    20941 tagagcagtt gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatg
    21001 taaaaaagcg ccctaaaggc gcttttttgc tatctgcgat actcaaagat tcgaacctgc
    21061 agcaggtttg agttgagcgc agcgaaacaa cggagccgct cgcggcgacg gcccgaaggg
    21121 cgagcgaagc gagtcatcct gcacgaccca ccaatgcaaa aaagcgccct aaaggcgctt
    21181 ttttgctatc tgcgatttgt aagattgatt tttcttctca agttccttgt tcttctaccg
    21241 atattacctc gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc
    21301 cttccataca aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcacaaa
    21361 tcgcgcggat aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca
    21421 ctagcggcag tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc
    21481 tactggaaac acaactggcg cagttgcaga aacagcaggc agaaaaggcg caggagaaag
    21541 agcagcgttt gtctctgaat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg
    21601 atatctatat ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt
    21661 ctcgtaccac gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca
    21721 ccagcatcaa ctggcgttca accaccggcg taatgcgctt aaccaccagt gggctacctt
    21781 caggcaatgg tagcgccagt gcaggaagaa tactgatgcc gatgcctgcg gctaccatcg
    21841 ggaacagcgt cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg
    21901 ccagcgctgc gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg
    21961 ctccctgtaa tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc
    22021 aaagcagaaa gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg
    22081 gatcgatgac gatgccaaag tcgacatctc cctggcgaat actttccatc acccactgct
    22141 gcggacgatc atgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc
    22201 attgcggaat aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca
    22261 gttgttgccc catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct
    22321 caagacgcaa agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt
    22381 ccagcaggcg aacaccagta tgattttcca gctccttcac actgtgactt actgccgact
    22441 ggctcaagcc gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga
    22501 aaacctttaa ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa
    22561 tcaattttat ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt
    22621 atgaaacttt ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg
    22681 gcctctttct ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca
    22741 gctattgccc tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct
    22801 ggcggtggtc actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg
    22861 attctgggtg tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc
    22921 ggttttctct acttgtgcat tctcccggct accgtgcagt ctgcaatcgc cttcacgtca
    22981 atggcgggcg gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg
    23041 attttccttt caccattgct ggttggtctg gtgatgaatg ttcacggtgc aaggggcagc
    23101 cttgagcagg tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt
    23161 tcccggccgt ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac
    23221 cagacgtcca ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc
    23281 tggcataaag ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg
    23341 gctatcgtga ttgtagttaa cgtctttatg gcacgccgac tgagcttcaa taaggcagat
    23401 gaaattacta tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca
    23461 aacattctgt tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat
    23521 cagatccaat tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag
    23581 ttacaggcgc agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg
    23641 accagttgcg tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc
    23701 ttccctgccg ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtca
    23761 attaagcgac gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc
    23821 tgctcttttt ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc
    23881 aatgcccagt tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag
    23941 atcggttttc ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt
    24001 agcgtcatca cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa
    24061 cgggctgtca atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc
    24121 caacagctcg ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga
    24181 tgcaacgaca atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag
    24241 cgcttccagc gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg
    24301 gatgccaagt gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt
    24361 aaccacgttt tgtgccaatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt
    24421 gagtttgaac agatctgccg gagtatggac atattctttt tcaaccagct gatcgatgat
    24481 tttgtcgccc attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc
    24541 tttacgctgc gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac
    24601 acgctcaacg tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc
    24661 ttccgggcgt tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg
    24721 aatcaccact ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa
    24781 ggttgcgtta ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt
    24841 aatcgcgcca gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg
    24901 cgccgggaat ttaaacgcaa ccgcccagcg cggggcacgc gcgacaaagc caagctgctc
    24961 ctgctgttcc agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg
    25021 gcggtcttct tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt
    25081 tacccgatcg ctgaccggca ccccccactt tttaaattgc agtaaacggc caagatgagt
    25141 atccggcagc tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg
    25201 cttcgctgta atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt
    25261 agcaaaaact ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg
    25321 cggcaggaac acttcaccac gcacttccag acgcgccggg atattctctc cgtgcagctt
    25381 cagcggaatg gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc
    25441 gccacgggtc gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc
    25501 atccagcttc agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac
    25561 acgtttgttg aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg
    25621 tacttcatgg cgtatctggc tgaaagctgc cagcggcgca gcacctacac gttgggtagg
    25681 cgaatcaggc gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag
    25741 cctgtcgtat tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg
    25801 atggcaaagc gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca
    25861 tcaatgctaa aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt
    25921 atcaggcgtt ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg
    25981 gagtcatcat acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct
    26041 gcgcagattg cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca
    26101 taaagatagt gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt
    26161 tcaccatatt cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat
    26221 ggtaaatatt catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt
    26281 caccgtttag ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc
    26341 gcttcggttt atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta
    26401 cgggttctgc aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg
    26461 gcgctggcgc gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat
    26521 aggcaggctg ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt
    26581 caggcggctg ctggaccggt tgacgcggct gcgcagacgc ataaggcggt aggtactggt
    26641 gttgcggcga cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc
    26701 ggtgaacacg aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt
    26761 cgtcacgttt tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt
    26821 tacggctggt ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga
    26881 ttaatatcag acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc
    26941 acggcaaaca tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt
    27001 aactttcaca gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc
    27061 taactgtcca ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg attggtcaaa
    27121 aggagctcat cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact
    27181 attttgcgca aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac
    27241 cgctgctggt caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg
    27301 atgtctggat cccgactttc atgagttacg ttccggactg gctacaatgg ctgagttatc
    27361 tgttgtggcc tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga
    27421 ttgctaactg gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat
    27481 tgactggcgc tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa
    27541 tcatgaaacg cgaatggcaa aaatttgtct ggtatctgcc gcgcgcaatt gtattactaa
    27601 ttctttactt catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta
    27661 gcgcctggat gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt
    27721 ttaaagagat gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt
    27781 taaccagcct gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt
    27841 gtggcgcgac ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac
    27901 aatctaccgg ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt
    27961 attccatctt gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc
    28021 gcatattctc tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag
    28081 gccatgagta agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc
    28141 ctgaatcgca tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc
    28201 agcgttaagt gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg
    28261 aaaccaggcg ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat
    28321 gtagctgccg ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa
    28381 cgccgcaagc tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc
    28441 atgaaaggcg caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg
    28501 ctgctgcaac aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg
    28561 gagatctggg aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc
    28621 ggtacgctga ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc
    28681 tctgtcgccg ttgagccaac cgattctcca gttatcgccc aggtgctggc aggtgaagag
    28741 attaaacctg gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc
    28801 gatctcaagc tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc
    28861 cgtcgtctaa tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc
    28921 gcagcgttga aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta
    28981 ccatcatcgg gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa
    29041 gaattgcaac agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct
    29101 tttttgtggc ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg
    29161 gtatttaacc agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag
    29221 ctgaatcgat tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt
    29281 acgaaacgtc agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg
    29341 cgtctgttaa aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt
    29401 agttccacaa cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca
    29461 ttaccgctcc gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg
    29521 gcttcacttc tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt
    29581 ttaaactgca gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg
    29641 aagacgagca gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc
    29701 ccgggttctt ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc
    29761 atccccgggt atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga
    29821 ccggaaaaaa atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg
    29881 tgccaaggca tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga
    29941 agaaaaagaa gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca
    30001 ggaaatcata gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt
    30061 tatcgaaggt caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc
    30121 ggctgacgta cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat
    30181 cgacctgagc gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga
    30241 aaccgcacag ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac
    30301 ttcccacacc tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag
    30361 cgtcacctct caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt
    30421 ttacgtcaat ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc
    30481 ttctgaaaaa gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca
    30541 ccaggtagaa gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa
    30601 cggcgctgaa ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct
    30661 gcccactgaa gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca
    30721 ggcggttatc gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt
    30781 cccgaaagaa gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa
    30841 agagatcctg cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg
    30901 cattatgttc ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga
    30961 aatctacaaa caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg
    31021 cgtaatggtg gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga
    31081 tttctttagt atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa
    31141 tgatatgatt tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca
    31201 agttattgat gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg
    31261 cgatgaacgt gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc
    31321 catttctatc ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt
    31381 gttagcagag caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa
    31441 gttcattgaa gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg
    31501 agaagatcat gggtttgttc gataaactga aatctctggt ttccgacgac aaaaaggata
    31561 ccggaactat tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc
    31621 cggatgtcgt ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta
    31681 acaaaatggt cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt
    31741 tctctatcga atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg
    31801 aactgaaagg cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg
    31861 atactgtcat tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc
    31921 cggttgttat ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtaa
    31981 ccgtgggtga aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg
    32041 cgcccatcgg cgccattttt tatgcttccg ccagcggcgg caaaatcaat tcatcgctct
    32101 catgctgctg ggtgtagcgc atcacttcca gtacgcgcaa ccccgcacgg tgcaccgcat
    32161 cgtttaacgc cttccctttc agcaaaccac tgataagctg agcacaaaac aggtcgccag
    32221 tccctttcag gtcggttttt acccgtgaat gggaaatgac attcacgctg tcggcactga
    32281 ccaccaccac ctgcatctcc tgattttctt cattaccgga ggcgctggta atcaccaccc
    32341 attttaatgt gtctgaaagc agactttttg cggcagcaat ggcactgtcg agatcgcggc
    32401 aatttttacc ggtcaggatt tccagctcaa agatattggg ggtaattccc tgcgccagcg
    32461 gcagtaaata ttgtcgatac gcctcgggaa ggtcaggttt gacataaatt ccgctatcaa
    32521 tatcgccaat caccggatcg accatgatca ataggtcagg atggtctttg cgtagcgcag
    32581 tcagccactc ggcaaggatt ttgatttgcg atgccgttcc catatagccc gtggttacag
    32641 cacgaagttg gcgcagcgca tcacgctcct gaagcgcacg caaatagccg ctaaaccatt
    32701 cgtccggaat cgcaccaccg tagaaagtgt cataatgcgg cgtattgctc agcaataccg
    32761 tcggcacggc aaagacattc aagccgttct gttttatagc aggcaccgca atgctgttgc
    32821 ctacgcttcc gtacactacc tgcgactgca cggcgacgat atccgcctgc agcgccctac
    32881 tcttatcgtt aaacaacaac aaactactca tttaattttt tctccttgcc gatgatcctc
    32941 atcgtattcc aaccgaaact ttacctgatt ctggcagtca aatcggctat tacaaaacaa
    33001 agataaggta attcagtgaa gaaaatcatt tgtctggcca ttacgctgct aatgacactc
    33061 cccgcttacg cgaagttaac tgttcatgaa gaagcccgca tcaacgccat gctggaggga
    33121 ttagcacaga aaaaggattt gatatttgtg cgcaacggtg atgaacatac ctgcgatgaa
    33181 gcggtttccc acctgcgtct gaagctcggc aatacccgta accgcattga cactgccgag
    33241 cagtttattg ataaggtagc ttcgtcgtca tcgattaccg ggaagccgta tatcgtgaag
    33301 atccccggta agagcgatga gaacgcacag ccttttttac atgcgttaat tgcgcagacg
    33361 gataaaacgg tgcctgcgga aggaaattaa tccgctttgg taaggcattt acaggaggta
    33421 acatgaaaaa atgctttatt tatcacgatg aaaaatcgaa taaattttgg tggatagatt
    33481 acgaagggga tagtttagct gtcaactatg gcaaggtagg tagtattggt aaattccata
    33541 caaaagaatt cgataatgaa gaacagtgtc tgaaagaagc cagtaaattg attgccgcaa
    33601 aaatgaaaaa aggatatcaa gaaactccag actttaactt catggatctc tactattttg
    33661 atgatgaaga gattgggtta catcttaaaa cgtcacaccc aaacttccag tgccatttta
    33721 ctgatccact ttatatgtgt tgctgggatg aagaatctcc ttttggtagc gatgaaggtg
    33781 ctgatgctct aaacgaactt gaaaatagcc tccgttctgg actgtgctga tttccctcaa
    33841 atgttaattg aaactatgtg gggtatgaaa tacatcgcta tggacagtat tcttgaagag
    33901 gatgtgcgtg cgcaattact agccgatgaa atgagctcta tccagagcaa tatgattacc
    33961 tacgcgactg cattcggtca gatcaaagtc atgggtaaaa tctcccataa acttaaaaag
    34021 atgggactca atgcactagc gcgtcatcag cttaccgcaa aaattcttca atggggtgac
    34081 ggtcaggact caccaatact tcaaaaaatg attgatgacc ttactgcttt tccacacgaa
    34141 aattaaaaac tgcatttgtc ggtagcaaca actgccgaca aatcagatta ttgacaaagt
    34201 gcactttgtt tatgccggat gcggcgtaaa cgcctgatcc ggcataaaaa tattgctaat
    34261 tcaatatatt gcagaaacat tgtaggcctg ataagcggag cgcatcaggc agttttgcgt
    34321 ttgtcatcag tctccaatgc tattaatcct taaatccctg ccccctgact aaaatgctct
    34381 tccccaaaca ctccggtgga gaggtagcga tcgccacgat cgcagatgat cgctaccacc
    34441 accgcaccag ggttagcttt tgccacccgc agtgctccag caaccgcgcc gcctgagctg
    34501 acgccacaga atattccttc ccgcaccgcc agttcgcgca tggtgttttc cgcatcgcgc
    34561 tgatgaatat ccagcacctc atccaccagc gcagcgttga aaatccccgg cagatattcc
    34621 gcaggccagc ggcgaatgcc cggaatactg ctgccttctt ccggttgcag gccaacaatg
    34681 gtcaccggtt tggattgttc gcgcataaag catgaaacgc cggtgatagt gccggtcgtc
    34741 cccatgctgg agacaaaatg agtgatgcgc ccgccggttt gctgccagat ttccggcccg
    34801 gtggtggtgt aatgcgcata agggttatcg ggattattga actgatcgag cagctttcct
    34861 tcgccacgat tcgccatctc cagcgccaga tcgcgcgcac cttccatgcc ctgctctttg
    34921 gtgacaagaa tcagttccgc accataagca cgcatcgccg cacggcgttc ctggctcatg
    34981 ttgtcgggca tcagcaattt catgcgatag cctttcagcg cggcaatcat tgccagcgca
    35041 atgccggtgt taccactggt ggcttcgatt aagacatcac ccggtttaat ttccccgcgc
    35101 ttttccgcct cgacgatcat cgaaagtgcc gcacgatctt tcaccgaacc tgccgggtta
    35161 ttgccttcca gttttaacca cacttcactg ccgttatccg gccccattcg ctgcaacttc
    35221 accagaggcg tattgcctat tgtttgttct aatgtactca caatctctat ccatacgtgg
    35281 tgttgcctga tgcgacgctt gcgcgtctta tcaggcctac aggttacaaa ccttgccatt
    35341 aaaaagcccg gatcgcggga agcgcctccg ggcgtttaac attcactcaa cctatcaggc
    35401 gctttgtgcg agagcaagtt cctcatcgcg ggtttcaata cgctcgtcgc cgttatacag
    35461 ccgcgcatgt tgcagaccaa cgaataaacg ctcgccatgc tgcggggcat cgtcgccatg
    35521 catcacgacc gtcagcggtt cgttgtacca ccccagcggc tgcaccacta attgggtgta
    35581 gtgacctttc gggctggctt ccagtacctg taccggcagc ggcgaatcga gactggtacg
    35641 gcggctgata tccacttccc acgggcgcag gaagagatcc accggcccct gatacgcagg
    35701 tgtgtagccc agcggccagc gatgcgcgcc aacatggaac tgcccgccgc gaatggttcc
    35761 ctgcaggcgg ttcacttcgc ccataaattc gagtacaaaa cgggtcgctg gttcgcgcca
    35821 tacctgattc ggcgcatcag cctgttcaat attcccctga ctcatcacca ctacacgatc
    35881 ggctacttcg gtcgcctctt cctggtcgtg ggtcacaaac acgctggtga atttcagctc
    35941 ttcatgcagt tgacgcagcc agcgacgcag ctctttacgc acctgcgcat ccagcgcgcc
    36001 gaacggttca tcaagcagca gaatttgcgg ttctacagcc agcgcgcgcg ccagcgccac
    36061 acgctgtttc tggccgccgg aaagctgcgc cggataacga tccgccagat gcgcaagctg
    36121 gaccatttcc agcaattttg tcactttcgc tttgatggct gcggcattcg ggcgctcgcg
    36181 acgcggcagc accgtcaggc caaaagtgat attgtcgaat accgtcatat ggcggaacag
    36241 cgcgtaatgc tggaacacga aaccgacttt acgatcgcgc gcgtgcaggc gactcacgtc
    36301 ggtgccgtgg aagcgaatat gcccgctggt ttgatgctcc agcccggcga taatccgcag
    36361 cagcgtggtt ttcccggaac cggacggccc cagcaacgcg accatctgac cagaaggaat
    36421 atccagtgag atatcgttca gcacctgggt gcgaccaaaa gacttcttga tattggcaat
    36481 ctcaatgctc atgatgctcc tcctgctgcg cgcgtttttc ctgattctcc aggcgccact
    36541 gcaacatact ctttaaaaac agggtgataa tcgccatcaa cgtcaacagc gcggcggcgg
    36601 taaaggagcc gacggtgttg tagtcctgct ccaacaattc aatctgtaac ggcagcgaaa
    36661 gtgtttcgcc gcgaatcgag ccggaaacca ccgacactgc accaaactcg ccaatcgcgc
    36721 gggcgttggt cagcaccacg ccgtaaagca gcgcccagcg aatattcggc aatgtgacgc
    36781 gacggaacat ctgccagccg gacgcgccaa gcaaaatcgc cgcttcgtct tcctggctgc
    36841 cctggcttaa catcaccggc accagctcgc gcaccacaaa cggacacgtc acaaagatgg
    36901 tgaccagcac cattccgggc caggagaaca taatttgcag attatgttca tccagccagc
    36961 cgccgagcgg gccgttggag ccgtagaaca gcagatacac cagaccggca accaccggcg
    37021 atacagcaaa cggaatgtcc agtagcgtca gcagtaactg gcgtcccggg aagttaaagc
    37081 gcgtcaccag ccaggccagc agaatgccga acaccaggtt taccggtacg gcaatcagcg
    37141 cgatcatcac cgtcagccag atggcgtgca gcatgtccgg atcggccaga ttctgtaaaa
    37201 ccggcatcag ccccttgctg aatgcctgca cgaagatgta aatcatcggc accagcagga
    37261 tgaacgccga aaccagcatc ccgatgccaa tcagaaacca tttgccccag ttaatcgggc
    37321 gcgcgtcata acgcttcaat tgggtaactt ccgccattaa tgacctacca cacgccgacc
    37381 aaagcgactt tgcagagtgt taattgagaa cagcagcagc agagatgccg cgaggatcac
    37441 cgaagcaatc gcgctcgctg ccgggtaatc aaactcctgt aagcgcacaa aaatcatcag
    37501 cgacgtcact tccgtcttcc acgcgatatt tccggcgata aaaatcaccg cgccaaattc
    37561 accaagacta cgggtaaacg acagcgccac gcccgccacc agcgccggag aaagctccgg
    37621 cagcaccact ttgcagaaac tctgccagcg cgttgcacca agcgtttccg ccgcttcttc
    37681 atattccggg cctaactctt ccagcaccgg ctgcacggta cgcaccacaa acggaatgct
    37741 ggtaaaggcc atcgccaccg caataccaag ccaggtatag gtgactttga tatcaaactt
    37801 cgccagccat tcaccgtaaa aaccgtttac ggaaaagagc gaggccagcg ttaaaccggc
    37861 gactgccgtt ggcagcgcaa agggtaaatc catcagcgca tcaagcagcg tgcggcccgg
    37921 aaagcgataa cgtgttaaaa tccacgccat cagcagaccg aaaacgccgt taaaaatcga
    37981 tgccacaaac gccgacagca gcgtcacttt gtaggccgcg accacctgcg ggttggtgat
    38041 cacctcccag tactgcgccc agctcatctc agaaagctgc atcaccagcg cagagagcgg
    38101 cagcagcaat atcaggcaca caaacagcag actggtgcca aggcttaaag taaagcccgg
    38161 cagcacgcgt cttgaagaaa ctgcaaacat tacttacgcc ccgccgctaa cagcttgtct
    38221 aactcgccgc cgctggtgaa gtgggttttc atcacttccg gccatgagcc aaatttgtct
    38281 tccacgcgga acagttcggt ctgcgggaat ttatctttca gtttgtccat gacttccggg
    38341 ttattcacac ggtagtaata gtcggtgatg atggtctgcg cctgtgggct gtagagccag
    38401 ttcaggtagg cttttgcggc tttttccgta ccgttggcct gcacattttt atcgacccac
    38461 gccaccggga attccgccag aatattggtt ttcgggatca ccacttcaaa gccctgtgct
    38521 tcatactgtt tacggatgtt gttcacttcc gattcaaagc taatcagcac atcgcccaag
    38581 ccacgctcgg caaaagtggt ggttgcgcca cgaccgccgg tatcgaacac ttcaacgttt
    38641 ttcaggaact gggtcataaa ctgttcggtt ttggctttat cgccaccgtc agctttatcc
    38701 gctgcgcccc atgccgccag ataggtataa cgcgcgttac ccgacgtttt cgggttcggg
    38761 aaaatcagct tcacgtcgga gcgcaccagg tcgttccagt cgtggatatt cttcgggttg
    38821 cccttacgca ccaggaagcc catggtggag tagaacggcg agctattatt cggcaggcgc
    38881 gactgccagt cagccgggat cagcttgcct ttgtcgtgca ggatttgtac gtcggtcacc
    38941 tggttataag tgacaacgtc tgcttttaag ccctgcaaaa tcgccagcgc ctgttttgat
    39001 gacccggcat gagattgttt tatcgtcagt ttgtcgccgc cgttatcttt tgcccattgc
    39061 tgttcaaacg gcggattcag ggcggcaaac agctcgcggg agacgtcata agaactgttc
    39121 agcagttccg ttgcctgtac atggcccgcc agcagcagag aagcgaccag cgcgagtgag
    39181 ttctttttca gtaagttaac ggccattgcg cacccttata aatttaatga ctttctaata
    39241 gccatcatat ttataacgga cgtgaaagga gtaacggttt tatataccgt ttggtgattt
    39301 ggaagttaaa aagggaataa gaacttggtt caacaggcgt aattgttgca ggcagtttgg
    39361 acacggacag cgcggagaaa ccggagcgta cacatagtac gtgaggattt cgagcactgc
    39421 ccggggccaa aatgacaaat gaaatagcct ggtgaactta gttcaagacg aaatcctccc
    39481 cacacatgca gggaggaaac agaggtgaat cagataccga cgctaaccgt ctccggcagt
    39541 gtgctgccgc catcaatcac attctgtgta ccggttaaat agctggattc atccgatgcg
    39601 aggaaggccg ccagttcgcc gacttccagc ggatcggcga ggcgacacag cgggattgct
    39661 tttgccattt cagtcagcac cgattctgga tcttccgggt tcgactggcg ggcaatgctt
    39721 tccgccattg gcgtgcggac gtatcccggg cagatggcgt taacgcgaat accggattgc
    39781 gcgtactcca ccgccagcga tttagtcagg ccaacaatcg ccgctttcgt taaggcatac
    39841 gccgtttcgc caggatcagc caccatatca ccagtgactg aagacatcat cacaatgcga
    39901 ccatctttgc gcgcaatcat ctccggcagc accgccttcg tgacgttcca tacgccttta
    39961 atattgatat caatatggaa atcgcgatct tcatcgctca tatcgaggaa actgcccaga
    40021 cgacaaacgc ctgcgttatt caccaggata tcaatgcgcc cttctttttc cttcgcgcgt
    40081 ttgatagctg cggctaccga cgccgggtca cgcacatcgg cgacaaccgc cgtacagcga
    40141 tgaccacgac cacacagttc gtccgctagc ttttcgatct caggggagat atccagcaag
    40201 attaggttcg cgccatgacg tgcaaaagtt ctggcaattc cttcgccaat tccctgcaat
    40261 gcgcccgtaa tcagtgctgt cttgcccgtg agtttaccca ttttaatgct ctccttgtta
    40321 atacagagcg ttacactcca ttccctctga aaatacagga taaacaaaat attgctgcgg
    40381 atcagctcac taattcagga aaatctcaat ccagtcgctg ctttatgcat catttcaggc
    40441 gttgtgttag ctcactgctg cgctgaatca ttttcaatga cgccacatca ttgatgttgc
    40501 acaagaccaa caaacagtaa atcggtaaca gaattttgcg ccgtgcgggt agacatcgac
    40561 gagctacgcc attcggtttc accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc
    40621 agcggagagt cagccagaga agtaatggca atcaccgttg ccccctgctt tcgtgccgct
    40681 tctgcgcaca agacaatctc ttttttactg ccgctgtaag agatagcgat ttgtacatcg
    40741 ccttttttca acgcctggga aacggtggct tgtacgtgcg tgtcggcttc gcaggccacg
    40801 cgatagccaa ttttcatcag tttgaacgac agatcgcgcc ccaccagcgc cgatccgccc
    40861 aggcccgtta tctggataaa cggtgcttta ctgatgacct caatgatttt ttgcagccgc
    40921 gcgtagtcga acaacgcaca ggtttgctcc agcgccagct ctttttcgcg attcagtttg
    40981 cgggcaatca cttccagcga atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg
    41041 gcattcgttt tctcccggct ggcactgtat tcgccaatta acgccatacg caattcagta
    41101 aatccttgcg cgccgagctt ctgggcaaat ttcacaatgc tcgactggct gataccgagc
    41161 tgtttcgcca tctgccgtga agagacggat ttcagctcgc tcactctggc ccgcagaaag
    41221 tcagcaattt tttgctcgtt cccggtaaat tcactttccg cattgcgtat tttcgtcagg
    41281 tacaacatct tccactctcc taggctggca gcaaaaattc cgcttaagtt cttatgacgc
    41341 tcttcacact ctgcgagtgt agactcaatg attcctttaa gtctgtcaat tcgggatata
    41401 tgattccata catctgcaat tatttgatcg taaatagtaa ggtcaccacc gatgcaacta
    41461 gaaaagatga ttactgaagg ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg
    41521 ctggaaatgt gccggattat caacgatgaa gataaaaccg taccgcctgc cgttgatcgc
    41581 gtcctgccgg atatcgccgc ggcgatcgat gttatccacg cccaggtcag cggcggcggt
    41641 cgtctgattt atctcggtgc gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa
    41701 tgtccgccca cctacggcgt gaaaccgggt ctggtggttg gtttgattgc tggaggcgaa
    41761 tatgccattc agcacgcggt ggaaggagct gaagatagcc gggaaggtgg tgttaacgat
    41821 ctgaaaaata ttaatttaac ggcacaggat gtggtggttg gcattgctgc cagcggtcgc
    41881 acgccgtatg tgattgccgg actggaatac gcacgccagc tcggctgccg cacagtggga
    41941 atttcctgta atccggggag cgccgtttca accaccgctg agtttgccat tacaccgatt
    42001 gtaggtgccg aagttgttac cggttcttcg cggatgaaag caggtacagc gcagaaactg
    42061 gtgctcaata tgctttccac cgggctgatg attaaatccg gcaaagtgtt cggcaacctg
    42121 atggtcgatg tggtcgccac caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa
    42181 aacgccaccg gatgtaatgc cgaacaagcg gaagcggcgt taattgcctg cgagcgcaac
    42241 tgtaaaacgg ccattgtgat ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc
    42301 ctggatcaac acggcggctt tattcgtcag gttttagaca aggaataacc catggccaaa
    42361 gagatcagca gtgaacttct gaacaccatt cttacccgtg tcggcggacc gggaaatatc
    42421 gccagttgtg gtaactgtat gacgcgcctg cgtctgggtg tacatgacag ttcactggtt
    42481 gatcccaata tcaaaacgct ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg
    42541 caggtcgttt ttgggcctgg taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg
    42601 ggcgatgcac cggtacagga tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa
    42661 gccaaacaaa cctccggcgt gcaacaattt ctcgccaaat tcgccaccat cttcacgccg
    42721 ctgatccccg gttttattgc cgccggtctg ctgctgggga tagcgacgtt aattgccacg
    42781 gtgatgcacg ttccggcaga tgctcagggc acgctacccg acgcgctgaa ttttatgaag
    42841 gtgttcagca aaggtttgtt caccttccta gtgattctgg tgggctataa cgccgcccag
    42901 gcatttggcg gcacgggcgt caatggcgca attattgccg cactcttttt gctcggttat
    42961 aaccccgctg caaccaccgg ttactacgcc ggttttcacg atttctttgg tctgcccatc
    43021 gatccgcgcg gcaatattat cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa
    43081 ggcatggtgc gccgctttat gccggacgat ctcgacatgc tgctgacctc gttaatcacc
    43141 ctgctgatca ccgctacgct tgcgtacctg atcattatgc cgctgggtgg ctggttattc
    43201 gaaggtatgt catggctgtt tatgcacctg aacagtaatc cgttcggttg tgcggtttta
    43261 gccgggctgt tcctgatcgc cgtggtattt ggcgtgcatc agggctttat tcctgtttac
    43321 ctcgcgttaa tggacagcca gggattcaac agcttatttc ccatcctttc aatggcaggc
    43381 gcgggccagg tgggcgcggc gctggcactc tactggcggg cgcaaccgca cagtgcgcta
    43441 cgcagtcagg tacgcggggc gattattccc ggcctgctgg gcgttggtga accgctgatt
    43501 tacggtgtca ccctgccccg catgaagccg tttgttaccg cctgtttagg cggcgcggcg
    43561 ggcggtttgt ttatcggatt gatagcctgg tggggtctgc cgatgggctt aaacagcgcc
    43621 tttgggccgt ctggtctggt ggcgctgccg ctgatgactt ccgcacaagg catccttccg
    43681 gcaatggcgg tttatgctgg cgggattctg gtggcatggg tttgcgggtt tattttcacc
    43741 acgctctttg gctgccgtaa cgtcaatctg gactgatatt atgaaacgga caatgctcta
    43801 tctttctctg ctggctgtta gttgtagcgt cagcgccgca aaataccctg ttctgacaga
    43861 aggtagttga tcaagtatat gcggcgttaa agcagtaata aaaaggccag tcagtaatgt
    43921 tactgcctgg cctttaatca atgaatcaga aacgcttaca gcgccatcaa cttgtccagc
    43981 gacggtgcga aataatagcc gccggttacc ggtttggtga aacgcaacat cgcatcacgt
    44041 ttgccatcgg tatcgccaaa catgctcagc agttgctgct caatgttatg cagacgcgcg
    44101 cagtaggcgc agaagtacag accgtgagtg ccactggcgg tgccgtacgg caggctctgg
    44161 cgaacaatct tcagcccttt gccatcttct ttcagatcaa cgcgggtgag gtgagaggtt
    44221 tccggacgtt cgtcgccgtc aatctcttcg ttggcctctt tggtgcgccc gatcatcatc
    44281 tcctgatcgt gaacgctcat ccggttgagc tgcttcaggt tgtgctccca acgctgcaca
    44341 aacacatagc tgccgccgga atccacgccg tctttgataa ccgccacttc gcggcgcgtc
    44401 tcttcacccg ccgggttttc tgtaccgtca acaaagccgc tcaggtcacg ctcttccacc
    44461 caacggaagc cgtggatctc ttctttcact tcaatgcagt caccaaaggc ttccatcgcc
    44521 gcctgagcga cagagaagtt tacgtcgtga cgcagagaaa gaatgtggat caacacatcg
    44581 aactgggtcg tcggcgcaag gcctttaccg tagcccggaa aatctttcag ctcttctgcc
    44641 ccaacgccgc cgctcagagc gcgccaggtg ttgttaccaa aggcaaccac cgcaccaaga
    44701 tgcgcgtccg ggaatttcgc ttcaaaagtt gccagtttgt cggcaaatgt tttactggcc
    44761 gcacgcaggg cgtcaacttc ccctttcacg ttggcttcga tccaaatcgc cgcgcggcaa
    44821 tgttctggca aaatgccact ctgaacctga gacattgttc ctcctgaaaa taagaatgcc
    44881 acgcaatccg tggcattgtt gagcgctatt ttacgtgttt tttcgcgacg gcggtttgtt
    44941 cagacgcaaa ttaacgccgc cagataattt tactgacttt ccagtttttc agggtatcgt
    45001 ccgaaggcat taacccttca ggaccacgcc attccccgct gaactggtag ctaatatgtt
    45061 gactgccttc ggctttacat tcgacggcac gattatcatc accatcagct ttttggcaat
    45121 tgccaaatgc tttgctgtaa aggtcgctaa acggtgtacc gattttaaca ccggtgtcag
    45181 caggaatatc gctatccagc acgtcaatgc gactgatcgt gccttgatcg ccgttaatca
    45241 ccatcgccac gttgtcgcct ttcatcactt caaaaaagcg caccacgttg ccgttcgtgg
    45301 ttttcattcc gctgcgcagg cgataatcgc catcaagcgc atcagcaatg gcttgttctt
    45361 gcagtggtgt ggacgccgtt aattcaccca cgccctgctc gctcactttg gtggacgatc
    45421 caaaccagtt ccacgggtta gcggcagacc agttaactga cgacatcgtg gaacagccgg
    45481 tcagcatcaa cggcatagcg cataacatta aacgcagcga tttcatgtca cttcctttgg
    45541 ttattcaata acgttgcttg gagtgcaaat tcaccaaaaa gtgccgttat tctttttctt
    45601 cattaaaaca agcgcgtaaa cgtcgattgg tcagcagcca gattaacgcc acaatatccg
    45661 ccactaccag cgccagaccg ataccgctaa cggattcacc gttcagccac agccacggtt
    45721 gccagcaaag taaaaccacc tgcgccaaca gcaacagaaa atagagcaca tgccaggtgc
    45781 gaggaaacga agcccgccga ccgctcaaca aaaacgccag caccgccgga ataccgggaa
    45841 tcagccccag ccagaaatta tcgtgatcgg gataaaacag atttagcagc gcagtaccct
    45901 gctcacgcga cgcaccggca atgacaaaca gcacccaggt tcgcgcctga agcaatagca
    45961 caagccagaa gagcaagggt aaacgcaggc gtccgtgcgc atcataatgg acaggatgga
    46021 actcagtact cttcatcttc aatcaaacgc ttacccagac tcagcacgtc ggcgtgttca
    46081 tatcccaggc gttcatacat tccgagcacc atgtcgttat cttccggcac gttgatctga
    46141 attttcgggc agccgcgagc aatcagcttt ttctccagcc ggttaagcaa cgcattggca
    46201 attccacgcc cacgaaactc tggatgcacg ccaagataat aagcagaccc gcgatgcccg
    46261 tcataaccgc ccatcaccgt tccgaccact tcaccgttta cctcagcgac caaaaacaaa
    46321 ctgacgtcat ggttcatctt acgctcgatg tccatttccg gatcgttcca cggacgcagc
    46381 aagtcgcaac gctcccaaag ggtgatgacc tcttcgaaat cttcctggcg aaatacgcgt
    46441 atctccatgg tattcgttac ctttttgcgg gttaaaaggc tgattatggc gtgaacggtc
    46501 gaattagcca atatctgacg aaaatcggtt gaaaaagtgg cataatgggg agttgtcaac
    46561 tattgaaatg aaaagtaaaa caattctcaa cagcaaaccg tcgtaacgga ttacgcgata
    46621 cgatataaca tctgaaactt tattattaca actcaggccg tatgagcact tttaaaccac
    46681 taaaaacact cacttcgcgc cgccaggtgc tgaaagccgg tttagctgcc ctgacgttgt
    46741 caggaatgtc gcaagccatt gccaaagacg aacctttaaa aaccagcaac ggacacagca
    46801 agccgaaagc caaaaaatct ggcggcaaac gtgtcgttgt tctcgatcca ggccacggcg
    46861 gaattgatac cggagcgatc ggacgcaacg gttcgaaaga aaaacatgtg gtgctggcga
    46921 ttgctaaaaa cgtccgttcc attttgcgta atcatgggat tgatgcgcgt ttaacgcgtt
    46981 ctggcgatac gtttatccca ctttacgatc gcgttgaaat cgcccataaa catggcgcag
    47041 atctgtttat gtcaattcat gccgatggct ttaccaaccc gaaagctgcc ggtgcttcgg
    47101 tatttgccct ctctaaccgt ggggcaagta gcgcaatggc gaaatacctg tctgaacgcg
    47161 aaaaccgcgc cgatgaagtt gccggtaaaa aggcgactga caaggatcac ctattgcagc
    47221 aagtactgtt tgatctggtg caaaccgata ctattaaaaa cagcctgacg cttggctcgc
    47281 atattctgaa aaagattaag ccggtgcata aactgcacag ccgcaacacc gaacaagccg
    47341 cgtttgtggt gttgaaatcg ccgtcggttc cttcggtgct ggtggaaacc tcgtttatca
    47401 ccaacccgga agaagagcgg ctgttaggca cggcggcgtt tcgtcagaaa atcgccacag
    47461 cgattgctga aggcgtgatc agttatttcc actggttcga caaccagaaa gcacattcga
    47521 aaaagcgata agttatgaaa cccgacgcac accaggttaa acagtttctg ctcaaccttc
    47581 aggatacgat ttgtcagcag ctgaccgccg tcgatggcgc agaatttgtc gaagatagtt
    47641 ggcagcgcga agctggcggc ggcgggcgca gtcgggtgtt gcgtaatggt ggtgttttcg
    47701 aacaggcggg cgtcaacttt tctcacgtcc acggtgaggc gatgcctgct tccgccaccg
    47761 ctcatcgccc ggaacttgcc gggcgcagtt tcgaggcgat gggcgtttca ctggtagtgc
    47821 atccgcataa cccgtatgtt cccaccagcc acgcgaatgt gcgttttttt attgccgaaa
    47881 aaccgggtgc agagcccgtt tggtggtttg gcggcggctt cgatttaacc cctttctatg
    47941 gttttgaaga agacgccatt cactggcacc gcaccgcccg tgacctgtgc cagccatttg
    48001 gtgaagacgt ttatccccgt tacaaaaagt ggtgcgacga ttacttctac ctcaaacatc
    48061 gcaacgaaca gcgcggcatt ggcgggctgt tctttgatga tctgaacacg ccagatttcg
    48121 accactgttt tgcctttatg caggcggtag gcaaaggcta caccgacgct tatttaccaa
    48181 ttgtagagcg acgtaaagcg atggcctacg gcgagcgcga gcgcaatttt cagctctacc
    48241 gtcgcggtcg ttatgtcgag ttcaatctgg tctgggatcg cggcacgctg tttggcctgc
    48301 aaactggcgg gcgcaccgag tcgatcctga tgtcaatgcc gccactggta cgctgggagt
    48361 atgattatca gccaaaagat ggcagcccag aagcggcgtt aagtgagttt attaaggtca
    48421 gggattgggt gtaactccct cacccccatt cccgcatccg ctgatgcaac gtcaacgacg
    48481 gcttctcggc aaacagctgc tggtaatccg tggcaaattg ccccagatgc cagaatcccc
    48541 actgcatggc ggcgtctttt accgttgtgc tttgcgacca cgggcttatc agttcgcggc
    48601 gtacgacgtt caggcgaatg cgtttcagcc acgcgtttgg gccaatgcct aaaatagcgt
    48661 gaaacgcgtt ttgtagcgtg cggcgactga catgcagttg attacacaaa tccagcaccg
    48721 tcaccggttc ggacatgttt tccagcacat attcacgggc gcgggaaagc aaccgacggt
    48781 aactctgatg actgatgctt tccgccgtca ccattggttg cgcttcttcc agcatcgccc
    48841 ccatcgccat tagcaaatta tcccccagca cttttcgcac tgcaggctga tggagatttt
    48901 ccgggttctc gcaaaacgtc gccagcgcct gttggacaaa gccccacagc gcggctttat
    48961 gctgctcttt cacttccagc gccgactggc tacgcagcat atgtaatacc cgatccggat
    49021 tgtgcaaaaa gttagcctga caggtgatga catcttcaga cagcactacg cccaggatcg
    49081 tataatcgtc cggcgtactt aattcaaact cggttccacc ggggcgcgtc gctatttctg
    49141 cgcttcccag acattgcgaa ccgataaatc cctgctcacc gcgcgtcgcc ggaatgccaa
    49201 accagaacga gttcggccag accaggcacg actgacgcag cgccagaccg gtgtattcac
    49261 gaaaaacctg aatatcatcg agtagaattt ccgtaaattc accatgaaac ttgcccggat
    49321 gcagctgatc gtaaatctgc tgctaggcgg taatcgtcaa agcatgttca tagacatccg
    49381 ttgtctgtcg ttgatgaaca ttatccactt cgaccttcgg cgtgagcttc aggttttcgg
    49441 gtaagggttc atgataaaga tggtgcaaat tggctgtacg ggtctttttc atgatgttaa
    49501 tgccgggtgt tgtaggacac ccggcacctc cgacaggtta atggggcttg agacgataac
    49561 gactactgcg tttacgtaac gtcccggcag aaaagagctg ttcgagcgca tttctggctt
    49621 tttccagcgg ccagccaaag tgggcggcca cttctcctgc cgtcattccc tgacatactg
    49681 atgttaacag cgccaacaac tcgtcagcag attccgcgac gataactggc gcgtcgggtt
    49741 cctttacggg ttgcttcggc tggcggttaa agccagtaac cagccactgg gtgtcatcgt
    49801 ctggccgacc gatctcctta cggctgatga cacgcccggt acgcatcgcg gcagcacaac
    49861 ctgcgtccag cgccgcacga cacgccgcca gatcgccttc caccaccagc gttaagcgac
    49921 cagggtcaag cacttcgtga ctgagcagac gaacgttagc tgctttgagc atggcatccg
    49981 ctgcatctat cgcggcgacc atgccgtcca cttccagcaa tcccagtgca ttgatcatcg
    50041 gcaacctccg ttacgcacgc tggattggat tacgcgcgat ttccagcact gcatcggtaa
    50101 aggcgttaca ggctgcttta cacgcggcct ggctaccggt taaaaatgct gccgagtagt
    50161 tggtttcaga cggtggcggg acgtaggtca ccagctgtac gtcggcagat ttcaacgcgg
    50221 catcaatgcc gtaggtcgct tccagcggcg gtgccaccag atacgccatc ggatcgccaa
    50281 gcgtgatccc ggcggttgat gaaagataag aaccggtacg cgaaaccaca tgcgccagga
    50341 aggcggtgtt ttctgcgtca ttggcccact ggaaagctgc gccattttca atatgtgcaa
    50401 ccatcgcatc cagaccggca cgcacttccg ccgggtttgg cccaccaagc ataatcagca
    50461 cttcaccggc agtcggtgac gggccgtgtg ccgctcccgc atacagcgag cgaccatata
    50521 ccacttcgac catcgcttgt ttggtcgctt cgtcagccgc aatgtaggtt acgtcgtcag
    50581 aatcggcaga aatcagcccg aggctacgaa tatgcggcgg caattttagt tcacgcgcaa
    50641 attcagcgtt aacggaggca atcacccgca tggcggttac cgacggtcga atcaaatcta
    50701 aagctggcat gatgcctcct tatcgggtca tgttgatgcc ggatgctttc tgctccagca
    50761 tacgtttcgc caaatccaca atgacggcac agcagcacga aagagaagca aaagtcagct
    50821 acctggtaca ctaggcagcg ggtcagcaaa ggggggcaaa tgcccctttt ttcatgtcag
    50881 ataatcagat cattacctca ataaactaat aatcacatcg cggttacaac cagtttgata
    50941 gtgatagctc aaacccagac gtggcgctgg ctgcaagcaa aataagccgc aaaaatgcga
    51001 ccccatgttt actagccgca aaaatgcggt cctatttgcc agaacctttt attattcaat
    51061 tggttagtgc aagaaaaagc gtgattactg ttcaagaagt atccagaaag gtcatgattt
    51121 tgacgctgaa aaaaaatagg tgggcatttt tgcccactca ctagccgcaa atttgcccac
    51181 tcactagccg catttttgcg gcccaaagta ataaatacag agaaataata aatacagatc
    51241 agcgccaaga tttttcgtct tttcggcgga atcatgagac tagtgcttac gtcctgataa
    51301 cctccagagc agaccttaac ttctgtgcag caggactacg ctcattgccg ctcggctagg
    51361 tcaaatcctc acgataaagc tgttcggatt gtgaccataa tgttaaagga ctctttaaat
    51421 cattccttga cgaattaact agctggctta agcggaatag catgcaggat aaaaacaaaa
    51481 ccagcttagc agtggggaga gcttcgcttc gctcgtctcc tggggccaat gctcaatgaa
    51541 acgacgtaag gcacggagcg ctcaccatag gtagacacat aaacatctga gagcgcacct
    51601 tctatcgcgc tacgcgctca ataaaaacca acgacaccgc cacactgcaa gaccaatggt
    51661 ttttatcgcg tatacagacc agacaggaaa cagcagcaaa gcagcgtata gccaggtgca
    51721 gacagcacac tcacacgacg cacagcaggc ttaagtgatc actcaggaaa ctttatcccc
    51781 gccgccgatc accgcgaact atcatgaccg cgccaaattc gaaaggaatg gcccatagaa
    51841 cgcgatgaac acacataagc gctacactta cttaacgtcg ccacagagcg attgagagcg
    51901 cttaaaatcg atgcgatttc attaaatttt tctcactcgc ctgaatggtg gcgatcagga
    51961 ctgatcgcaa cattgccccg cctcgatatt gagcgcataa tagatacata cattaagcgg
    52021 aaaagagata agtttattgt gaaaatcagg gggttacata tcatgcgcat atcagaacaa
    52081 caacggcaga tcctcgacta tctggcaaaa aacgaatcaa tcaccgcacg acaagccgca
    52141 gatctcgttt atggtggcaa tgttacccgc gtccaggttg acgccgcccg ccgctcactc
    52201 atgacgatgg ttagtaatgg gctaatcagg aaacaaggcc gcgctttcgt agctgaaaga
    52261 catatcaatc acgcttatag ccaggacgtt gaagaattgc gccgccagat cctttgggta
    52321 ttcagcaaac agggtcagga gtatttcctt tctgaacacc caaatttcgc cgccaaagag
    52381 ttaacagtaa gcggattgtc gtgcgcactg gattatcttg tcggtgaagg ttgcgttact
    52441 tacaagcagc tatggcacgc cctgaatacg cttgtagaag atggtttact cattgccacg
    52501 cgtgagaaca tcgaatactg gataatgccg cgttcaggtc gccgcaatca tgcacggtat
    52561 atttcaggca acagatacag aacgccggac tttaatccga cacaggaaga tcgggaccgt
    52621 gccgaaatga tgtgcaggga acaggagaaa agaattatgg ataagttatt ttcgatgcag
    52681 tgaacacaag cgggcgcaat gcccgttttt attgccttat tccacgcctg acgaatcaat
    52741 ttgatcacac ctgagaaaat gcatagccag aagttaaggc cgttatattg cgattgggag
    52801 cgttttaaac ggtatcaatt taattaattc tgatgtggtg atttgcaggc aaaaaaaacc
    52861 cgccggagaa gcgggaaata gagacatatc gttgtagtta ttaacagatc actttgaggt
    52921 cgagggcggc taaccctcgc taatattatc ccgctattac cttgcgggtg caaataatct
    52981 tttattgctt tgatgattta ataaatagca ctttttgcta acaaaaaccg ctgtcaatat
    53041 atggccaaaa atgtcaacag aaaaaataag gtttataata ttaagtatca catgggtaat
    53101 gggttcaggg aatcggaatc caccgcctca cggaaattca aaatttgctt cttctttcct
    53161 ccgtgggcgg cttctttttc gtatcgaggt atttatgaaa gcaataacat tagaacagtt
    53221 cgcagcaatg gcgaatgtat cgaccagaac gctgaggcgc tggctaaaaa aaccatcaac
    53281 cggagtccct ttgccattta ccccacgcgg tactcgccaa ttattctggg agcacgaatg
    53341 caaagaatgg ttgtacagaa cagaacgata ataatcacgc atcacatttt gaggtaaaca
    53401 tcatgaccaa tatcgaaaga ttagaacgct gtgccgatat tatgcgacgc agatggattt
    53461 atgaccctga agcgggttta ttgtttagcc gtgaaacaaa agacgttatc aaaggcagca
    53521 tgaccaataa agggcatttg gttgtaacgg tacatgaagg taaattcaaa gtgacactca
    53581 cctatcagaa agcctgctat gtatacacat acggcgctta tgacgagaca ttatatgaag
    53641 tgcaccacgt taatttgaac aaacaggata accgcatcaa gaatattcgc ctgatgccga
    53701 aagaaaagca ccgccggata cataaaaacg tccgcaaact tattcggctg ggccacacac
    53761 aatggcttca accgcaatta attgaacaat cactataaac acaggagtaa ataccatgca
    53821 ttcattagac agttattttc agcgcacgac agcccctaaa tcagccgccc aggaacgccg
    53881 tgaggaattc catgagaaag tgatgcgttc tgctgattat attgcggaca agttcgtgga
    53941 aacggttcgt ccacttgttg atgaagtagc tgataaactt cagtctgaaa tgccggaaga
    54001 tatggaaggc actgcaaagc ggcgactcat ttgcgaatta agtcgtcgtt ttggcgtgtc
    54061 aataagcgct tttaaataat cgaaaaaagg ggggaaagat aatggacgca tttgaggtac
    54121 tggcaaataa aggcgctgaa ctggtagcac agcgtgataa aaccgccaac gaaggggaac
    54181 gcgcactgct aaataaacag attaaagcga tccgtatggc ccagtttaaa ttaatcagta
    54241 atgaagttat cgaaacaatt aaaccgcaga tcccacaaat cgttgctgac gctattaaag
    54301 ccgccggact ggagaaacgc attgccgggc taaaaacgaa cgcagagaaa atggagatgt
    54361 acaaacagag cggtgctccg aatcccgatg agtacttctc accggatgtt gagtttatgg
    54421 cgcaggttga ggaacgtctg ggtagctgtc tcaccgagga acagcgtcgc tattttgatg
    54481 gagtggatag ttcggcaggt attgacctga actcgtattt cggtcgtgaa attgagcatt
    54541 ttgatgccgc cccgtctatg ccggagccag aaccagaaat ggccaccgat cctgaagctg
    54601 agcaacggaa acgcgtattc aatgcgatag ggattcggta ctgatcggat aaaagaaagc
    54661 cgggaattac cccggctgac ttaacaatgt gaccaatgaa caaatcggac aagacaaaca
    54721 ggataagcag tctcgaaaca atgttcggga actatattag cacaagtccg gagaaaatca
    54781 tcatgttggc actacgcatc atgcagggca tcgctaagac cctcgcagaa catgtcttag
    54841 acctgaaaca ttcgccactg agcaaacagg ccatgaagcg ccaaacgctt cgtctgtggg
    54901 ctgaatattc gcttggaact atcaataaaa tcatcgacat gaaatcgggg ccgagcaacc
    54961 agagcgcgga ggaaatggag ttcatcaggc gtttaattct cattcgcaga gacattcata
    55021 gccagttaca cagcgtggga atcgatatta atgatgggac gggcgattaa ttttcggaca
    55081 agtactatgc gcgcgcgagg agctgaacat gaagcaagat attgcagaca ggctggagat
    55141 actcgaaggc cagcgggcag aagcgaaaca actacgtaag caggccagaa gagcgcacag
    55201 gaacaacgaa gcagagttac taaccaaata catcagtttc accaattact gcatctacga
    55261 gtgttacaag gaagatgcag aggactggct ggattcattg ccagaacagt actgagcaga
    55321 ataacaccac gtcatgttgt tctgaggtga gcatatcacc tctgttcagg tggccaaatt
    55381 tagtgtgcca cttcagcatc accgttgaaa tcacaataac gatttttttg taaatatgct
    55441 ctcacatatg taggaataga ccaggttggc attgaaacac atctaccatg accataataa
    55501 agtaattttt t
//
LOCUS       DAZOYF010000008        54400 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZOYF010000008 DAZOYF010000000
VERSION     DAZOYF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.008"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="ACHUOF_000491"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="ACHUOF_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR0609814.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="ACHUOF_000492"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="ACHUOF_000492"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR0609815.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="ACHUOF_000493"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="ACHUOF_000493"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR0609816.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="ACHUOF_000494"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="ACHUOF_000494"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR0609817.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="ACHUOF_000495"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="ACHUOF_000495"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR0609818.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="ACHUOF_000496"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="ACHUOF_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR0609819.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAATPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="ACHUOF_000497"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="ACHUOF_000497"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR0609820.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="ACHUOF_000498"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="ACHUOF_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="ACHUOF_000499"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="ACHUOF_000499"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR0609821.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="ACHUOF_000500"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="ACHUOF_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR0609822.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="ACHUOF_000501"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="ACHUOF_000501"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR0609823.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="ACHUOF_000502"
     CDS             complement(13538..13933)
                     /locus_tag="ACHUOF_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR0609824.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="ACHUOF_000503"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="ACHUOF_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR0609825.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="ACHUOF_000504"
     CDS             complement(15220..15447)
                     /locus_tag="ACHUOF_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR0609826.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="ACHUOF_000505"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="ACHUOF_000505"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR0609827.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="ACHUOF_000506"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="ACHUOF_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR0609828.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="ACHUOF_000507"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="ACHUOF_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR0609829.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="ACHUOF_000508"
     CDS             complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="ACHUOF_000508"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR0609830.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19688..19764
                     /locus_tag="ACHUOF_000509"
     tRNA            19688..19764
                     /locus_tag="ACHUOF_000509"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19722..19724,aa:Met,seq:cat)
     gene            complement(19839..21092)
                     /gene="amiC"
                     /locus_tag="ACHUOF_000510"
     CDS             complement(19839..21092)
                     /gene="amiC"
                     /locus_tag="ACHUOF_000510"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR0609831.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21324..22655
                     /gene="argA"
                     /locus_tag="ACHUOF_000511"
     CDS             21324..22655
                     /gene="argA"
                     /locus_tag="ACHUOF_000511"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR0609832.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22717..24543)
                     /gene="recD"
                     /locus_tag="ACHUOF_000512"
     CDS             complement(22717..24543)
                     /gene="recD"
                     /locus_tag="ACHUOF_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR0609833.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24543..28085)
                     /gene="recB"
                     /locus_tag="ACHUOF_000513"
     CDS             complement(24543..28085)
                     /gene="recB"
                     /locus_tag="ACHUOF_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR0609834.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28078..30966)
                     /gene="ptrA"
                     /locus_tag="ACHUOF_000514"
     CDS             complement(28078..30966)
                     /gene="ptrA"
                     /locus_tag="ACHUOF_000514"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR0609835.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31142..34510)
                     /gene="recC"
                     /locus_tag="ACHUOF_000515"
     CDS             complement(31142..34510)
                     /gene="recC"
                     /locus_tag="ACHUOF_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR0609836.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34523..34882)
                     /locus_tag="ACHUOF_000516"
     CDS             complement(34523..34882)
                     /locus_tag="ACHUOF_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR0609837.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVLMVMIVTALSG
                     IQRTSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVS
                     PGGREGEMTRLHCPNCQ"
     gene            complement(34867..35274)
                     /locus_tag="ACHUOF_000517"
     CDS             complement(34867..35274)
                     /locus_tag="ACHUOF_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR0609838.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35271..35834)
                     /locus_tag="ACHUOF_000518"
     CDS             complement(35271..35834)
                     /locus_tag="ACHUOF_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR0609839.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35825..36295)
                     /locus_tag="ACHUOF_000519"
                     /pseudo
     CDS             complement(35825..36295)
                     /locus_tag="ACHUOF_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36479..37273)
                     /gene="thyA"
                     /locus_tag="ACHUOF_000520"
     CDS             complement(36479..37273)
                     /gene="thyA"
                     /locus_tag="ACHUOF_000520"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR0609840.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37280..38155)
                     /gene="lgt"
                     /locus_tag="ACHUOF_000521"
     CDS             complement(37280..38155)
                     /gene="lgt"
                     /locus_tag="ACHUOF_000521"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR0609841.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38306..40552)
                     /gene="ptsP"
                     /locus_tag="ACHUOF_000522"
     CDS             complement(38306..40552)
                     /gene="ptsP"
                     /locus_tag="ACHUOF_000522"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR0609842.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40565..41095)
                     /gene="rppH"
                     /locus_tag="ACHUOF_000523"
     CDS             complement(40565..41095)
                     /gene="rppH"
                     /locus_tag="ACHUOF_000523"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR0609843.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(41448..41594)
                     /gene="ygdT"
                     /locus_tag="ACHUOF_000524"
     CDS             complement(41448..41594)
                     /gene="ygdT"
                     /locus_tag="ACHUOF_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR0609844.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     HGIG"
     gene            41780..42469
                     /gene="mutH"
                     /locus_tag="ACHUOF_000525"
     CDS             41780..42469
                     /gene="mutH"
                     /locus_tag="ACHUOF_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR0609845.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42538..43251
                     /locus_tag="ACHUOF_000526"
     CDS             42538..43251
                     /locus_tag="ACHUOF_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0609846.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43389..43607
                     /gene="ygdR"
                     /locus_tag="ACHUOF_000527"
     CDS             43389..43607
                     /gene="ygdR"
                     /locus_tag="ACHUOF_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR0609847.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43715..44755
                     /locus_tag="ACHUOF_000528"
     CDS             43715..44755
                     /locus_tag="ACHUOF_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR0609848.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44788..45978)
                     /gene="lplT"
                     /locus_tag="ACHUOF_000529"
     CDS             complement(44788..45978)
                     /gene="lplT"
                     /locus_tag="ACHUOF_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR0609849.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(45971..48130)
                     /gene="aas"
                     /locus_tag="ACHUOF_000530"
     CDS             complement(45971..48130)
                     /gene="aas"
                     /locus_tag="ACHUOF_000530"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR0609850.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48716..49747
                     /gene="galR"
                     /locus_tag="ACHUOF_000531"
     CDS             48716..49747
                     /gene="galR"
                     /locus_tag="ACHUOF_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR0609851.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49754..51016)
                     /gene="lysA"
                     /locus_tag="ACHUOF_000532"
     CDS             complement(49754..51016)
                     /gene="lysA"
                     /locus_tag="ACHUOF_000532"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR0609852.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51138..52073
                     /gene="lysR"
                     /locus_tag="ACHUOF_000533"
     CDS             51138..52073
                     /gene="lysR"
                     /locus_tag="ACHUOF_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR0609853.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52060..52752)
                     /gene="ygeA"
                     /locus_tag="ACHUOF_000534"
     CDS             complement(52060..52752)
                     /gene="ygeA"
                     /locus_tag="ACHUOF_000534"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR0609854.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52881..54299)
                     /gene="araE"
                     /locus_tag="ACHUOF_000535"
     CDS             complement(52881..54299)
                     /gene="araE"
                     /locus_tag="ACHUOF_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR0609855.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        13295 a        14732 c        13415 g        12958 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 cgggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gacaaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagccagg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa ccccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttctaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggct gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgttggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgccgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgaat
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat ggcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcaggggac ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgaa gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcccctgtca tatccgtaaa
    18361 aacggcatac agaatatcac atcagccgct aaagacgtta cctgcgcccg gagccgtttt
    18421 cagcacccaa acccgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc
    18481 gatcccttga tagatatcga agtgttggcc tttgattgcg cccccgacat ccaacgccac
    18541 catcagacgc aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc
    18601 tgccagcaag gtagtacctg gcggaataat tgaacgatcg gaggcaacag aagcacggcc
    18661 aaccagcggc accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaagac
    18721 gaaagacggg ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca
    18781 atgacgaatc gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac
    18841 cttaccaatg ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg
    18901 actgccatca ccaaagtcga tatacccact accctgcaca tccataatga agttatccat
    18961 cagagagttg ctgtaagcga ggatgtattt atcgcccaat gccccggcgt agatctccgc
    19021 acgagacggc agacgaccac gttttggcgg catacggtaa ataggatact ggaactcgcc
    19081 ctggcgggta tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc
    19141 atagttgtcg gcaccttcca tctgccaggc atcaatgccg aactggcgca tattgcgggt
    19201 atcaccgcct gcgcgcagcc actcttgcac cgcgttataa acattactct ggttgccata
    19261 cagacgcggt gacgaattac ggatatggtt aatttgctcg gcaaaatcac cggcgttaat
    19321 cggcgcgcca acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcccgtc
    19381 tttatattgc tgtccgcgat cggttggttt ggaagagcag gcggcaagca ttgccacaac
    19441 cgtgcccata agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa
    19501 aaataaggcg caacgaagat aacaaaccgc cgcgtttaat gaaatgagca gcctctccct
    19561 gacgcaaatt ttgcacaaaa aacaggcttt agtggtttgt ttttgttcaa aatcatgcta
    19621 aatccgtgat cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat
    19681 ccacggacgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg
    19741 gttcaaatcc ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct
    19801 gatctcccat aaaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtt
    19861 gccccatcgg caaaatacgc tttaatcccc gcaagaatag actccgcaac ttcctgctgg
    19921 aaagtcgccg ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc
    19981 agaatggagg gaatatctgg tgcctttagt acggcaaacc cggcctgttc aacttgattt
    20041 ttatgcagct tgttgatttt tccgagctta ttcagtaccg ctttaccaaa cttaaggctg
    20101 tcagcaatag tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg
    20161 cttttgctca cgccaccaat caagtccgag gcgttctggg tttgtgccag atattttgcc
    20221 gcagtactgg ttgcgccttt ggttgagagc gcaaacaccg aggaaccgct cggctgacga
    20281 ctggtaaagg cgtcggcatg gatagagaca aacaggtcag cacgctgttt ctgggctttt
    20341 gctacgcgca cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata
    20401 ttgccctctt cctcgatcag agagcgcaaa cggcgagcta tttgcaatac cacatctttt
    20461 tcgcgcgttt tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc
    20521 ataatgacaa tcggacgatc gcgccctgct ttacccggtt gtggaccact ttgcgctggc
    20581 ggcacctgct tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc
    20641 tgcatatcct gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg
    20701 accggcgcaa gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt
    20761 acggtttgcg ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga
    20821 atttgtgccg ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc
    20881 acgcgttcag ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc
    20941 gtcacgcggg tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctaaccgca
    21001 gccaggctga cctgacttac gctcaatagc cacatagcgc ccgcgccttg cagtaaacga
    21061 cgacggctga ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc
    21121 tgtgtaacaa gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc
    21181 atctaataaa cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc
    21241 tcgcataaaa tttacacttg caccctgcga aaaaacagaa taaaaataca ataatttcaa
    21301 ataatcatgc aaaggggtgt gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat
    21361 tccgccattc ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg
    21421 gcggtgaagc cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc
    21481 acagcctcgg catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc
    21541 tggctgcgca tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa
    21601 cactggaact ggtgaagcag gctgcgggaa cattgcaact ggatattact gctcgcctgt
    21661 caatgagtct caataacacg ccgctgcagg gcgcgcatat caacgtcgtt agtggcaatt
    21721 ttattattgc ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta
    21781 tccggcggat tgatgaagac gcgctccatc gtcaactgga aagcggtgca atagtgctaa
    21841 tggggccggt cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg
    21901 ccactcaact agccatcaaa ctgaaagctg aaaagatgat tggtttttgc tcttcccagg
    21961 gcgttactaa tgacgacggt gatattgtct ccgaactttt ccctaacgaa gcgcaagcac
    22021 gggtagaagc ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg
    22081 gcgcagtgaa agcctgccgc agcggcgtgc gccgctgtca tttaatcagt tatcaggaag
    22141 atggcgcgct gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg
    22201 aaagcgccga gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt ctggagttga
    22261 ttcgcccgct ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg
    22321 aaatcgacaa attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct
    22381 atccgttccc ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc
    22441 gcagttcatc acggggcgag gttctgctgg aacgcattgc cgctcaggcg aagcagagcg
    22501 gcttaagcaa attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat
    22561 ttaccccggt ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc
    22621 gtaaatccaa agtgttgatg gcggatttag ggtaagcgat gaaaatcacc ggatgcggca
    22681 ggcgtaacac tcgtgcgtcg catccggcaa ttacgtttat tcccgcgaac taaacaacgc
    22741 cgccagacca ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tacgctcatc
    22801 ggcatacagc gacagacgac gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt
    22861 tactaccggc gtgcgttggc tcggtaaaat caacgccgca tggtcgaact ccgatccctg
    22921 cgatttatgt accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac
    22981 agacttaata ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag
    23041 cgcaataccg atatcgccat taaacaaccc aagcgcgctg tcattacggg caatcatcac
    23101 cggtcggcct tcgtaccaac gagagtgcgg atgacgatga attttgcgct tctgttgcat
    23161 aaactgttca attcgctcat tcagtccagc cacgccaaac ggcccttccc gcagggcgca
    23221 caaaagctgg tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag
    23281 cagatccaga taacgtccgt aacccgcaag agcttcctca agcatcgcaa tataatcttc
    23341 gccgctctgt aaaagtcgtt tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac
    23401 tgccgtttta tcaccacggt tgatcgccgc agctaactga ccaatgccag aatcgctgcc
    23461 gaaacgatag cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc
    23521 ggttcctgcc ggaacgtgag tccccgtcag gcggcttagc tgcctggcac gctcggcggt
    23581 aaagcccgcg ttggcataag cgcagatatc gcccagcaca gccccagcct caaccgaggc
    23641 cagttgatca cgatcgccga gaaagatcac tcgcgcatga tctggcaagg cgtcgatcag
    23701 tctcgacatc ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg
    23761 cagcgggtta ccggcatgat gacgtaaacg ctggctaccc ggctgcgcgc ccagcaatcg
    23821 gtgcaaagtg ctggcatctt ccggaatgcg tttcttttgt tcatcggtca gcggtaactg
    23881 tcgcaaagcc ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc
    23941 cagacggata cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gcaacttcgc
    24001 cacggtggtc gttttaccgg tgcctgggcc gccggaaatc accgagatcc gccgcgtcag
    24061 cgccactgcc gctgcaactt tttgccagtt aatttcatcg cttactggaa aaagtttgtc
    24121 cagggtttgc gccagtagag cttcatcaac ctcaatggta tgattcacct cattgaagaa
    24181 gcgcgctacc gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag
    24241 gatcatcggc gtgggttcat ctcccctgct gaccgcttga gaagccagca agcattcttc
    24301 ccaattttgt agctcaccga tttcactgac acaggtcgcc aacagcggat tcgacgcctc
    24361 gttattttcc agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat catgacttaa
    24421 cagtgccgcc gcgagggtga cggcaggatg ttcatctccc gccacggtca gggcaaattg
    24481 cacatccagc gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt
    24541 cattacgcct cctccagggt cataccggca aacatctcat ccatcagggc aatcaacccg
    24601 gcgttgggtc gggttgtgta aatcccttgt tgcggatgtt ctttatcaac gccacgcagg
    24661 aacagataga taacgccgcc aaagtgaaga tcatagtcgt aatcagcaat gcgatggcgc
    24721 agataacgat gcagcgccag ggtataaagc tgatattgca gatcatagcg gtgtgcctgc
    24781 atcgctgctg ccatagcctg ttgggtgtaa gccgaactgt cttcacccag ccagttggat
    24841 ttatagtcga gcaggtaata acgcccttcg tggcggaata ccaggtcgat aaagcctttt
    24901 aacatgccgc gtacctgcat gaactccagc ggcgggcagc cagcggatag cgggtcaaac
    24961 tggcgaatta gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg caggtaaaac
    25021 tccatctcca cctgtttatc gcggtcggaa agctgactca ggctaacacc cgtttcattg
    25081 agaggtgcct ggaggacagc cgtgatccac tcggtcaaaa ccggttccca ctgcgattca
    25141 aagccgccga gttccagttt ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg
    25201 aggtcttcaa acaaactgtg caagaatgtc cccggtgacg caccgcgcgg gaactgatgc
    25261 ggtgttaacg tcggttcttc aacgacgctg accacgcctg cggcatcaac atccagccgt
    25321 ggcatcagat cctgagcgat accgtgacca cgctgctgca agccggagta gctggtgaca
    25381 cgccagttat cgccgggcaa tcgttgtagt gtcctcgcat tcagttctgc agttaaagca
    25441 tcattaacct gccagggctg gttatcacca gtttgtgccg tttgccaggc aatatcatca
    25501 tcgcataacg cttcaataca ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc
    25561 agcaaacgcc cgagcgcact ttggtggacg tcggtgtcac cttttttatc gccacgacgg
    25621 cgcaccagcg gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa
    25681 agcaaacgca gatcttccgc cagacgttcg acctccgcga ggtcgacgct ttctggcgca
    25741 gcattaagat ccaaaactgc ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg
    25801 acgcggaaat tggtaataaa tggcaaccag accagcggat attccagccc tttcgattta
    25861 tggatcgtga caatctgcac cagatgttta tcactttcga ggcgcatttg ttggctggag
    25921 gcattactgt ctggctcgag gatatgttgc gataaccagc gtaccagcgc atgttcactt
    25981 tccagctgcg ttccggcttc ttgaagcagt tcgctgatat gcaagatatc ggtaagacgc
    26041 cgctcaccgc ctgccgttgc cagcaagttt tctgcaatgt tacgcgccga catcagcgcc
    26101 cgcagcatcg gcataacgcc acgtttgcgc cagatttgcc gataaccatc gaactcttcg
    26161 actaccgcat cccacgcatg ttcgtcatta ttcagcgttt caatatccag cgcattcagc
    26221 cccatcattg acgttgccag cgcactgcgc agggtgttct cacgttcggg cgtcatcacc
    26281 gcctgcaaca accaaagcat ttcctgcgct tccagagttt caaaaacact gtcgcggttc
    26341 gaaaggtaaa cggaagggat ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc
    26401 tggcggctgc gcaccagcac gctgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg
    26461 ttcatcagca acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca
    26521 catacctgcg ccatggtact ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc
    26581 cacattttca tcgcaggctg cgtttcacct ttaaatacaa aacgtaacgc ctgatttttc
    26641 ccggctgatt tcactggaat aaacggtatt tcgcgaaaca tgaacgtgtc atcagtctgg
    26701 ctgaaaagct tgttcacact gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa
    26761 gtgtagtggg catgaacttc actacgcgcc ttcatataag tgaagatatc cgcaccccgg
    26821 aatgcatata tggcctgctt cgggtcgcca atcagcaaca atgcggtttc cggctgatgg
    26881 tgccagatac ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc
    26941 attgccaccg ggaatcgcgt acggatcgcc gctgccaaca cctcgccgct ttcgctacgc
    27001 agcgcggaat cgagccgact taacatgtca tcaaaaccca attcgccacg gcggcgtttt
    27061 tcacgcgcta ctgtttcgcg gatctcagcc aatgcgcggg tgatcaacag atcgcggatc
    27121 gacaatggtt ctgcaagcag ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc
    27181 cccccggcct tcgtgcgatc ttctaagaaa cgctgggaga atttttccag cgactccggc
    27241 aactgataac tgtttgtctc ttcttctgcc caggcgctga tcttttcgat ccatttagcc
    27301 tgattgctac ggttaaactt gcgtcgatca ataccagaag attcgatcag cgcatccagt
    27361 tcacccactg cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg
    27421 tggcgggaag ccagcgtttc atcatcgggc ggcggtgctt tgataaccgg cggttcgcct
    27481 tgcagataac gattaatatc gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg
    27541 acctgggcta tttcacgcgg cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc
    27601 tggtagcgta gcagagactc atcttcaatc agctgctgct caaacagcat gccggattca
    27661 aaggcattca ggttgagcat gcgctggcaa aagccgtgaa tagtaaagac tgccgcttca
    27721 tccatctgcc gttcggctaa caacaaccac tgcgcggctt gcgctttatc gtcgatctct
    27781 tccagcaggc gttcgtacag tggattgtcg gtggtttcac gcagacaggc gatgcgcaac
    27841 tcgtggatat tgctacggat acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg
    27901 accaccagca gttcttcaac gatcagcggg cggggaaagg cggcggaacc gcctagtcca
    27961 agtaacaggc gcaaatagag cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct
    28021 tcaatcaggc gctcgccctg taagggcaag cgcaaaggat ctagtgtctc ggcgacatca
    28081 ctcattcttt tcactcatca ggggcattgt ttgctgcaac gcgctgacgt tctcccacac
    28141 tttccagcct tcaggatgca catattcggc tttcccgttc tggctgccgg aaatctgcga
    28201 cagaatagcc atgccttgcg gctcgaccac cgcctgatgg aagaaatcag caagtttttg
    28261 cggcgtcagc agttttatct gggccacgat tttatcacgc gaatcgaagc gcatattgcc
    28321 gcgatcgaaa tctttactta acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag
    28381 catctgggta attaccgcct gctggatttg cgcaaactca tctggcttca tcgctcgcaa
    28441 ttttgcctct gcggttggga aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt
    28501 atcattgctt tgcaaaagga agcccatgcc ccactgacgc cccacgctca ttggaaacgc
    28561 aaacacggca tagcccaatt gttcttcggt acgcaactga ttgtagaacc acggctgtac
    28621 gatctgcccc aacagagagc tataggctga gctggtgtat tcatcgtagc cagtcggtac
    28681 aaataccgct gccagtgcgg agtcggtgct gttaccggct ttttcaaaga tgacggattg
    28741 ttttttatcg accactacat ctttgtttcg acaccactct gaaccatcag cgcccaactg
    28801 tttttgcaca tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat
    28861 aaactctggt cgagcccctg attttaaggc gtcgcgatag gccagcacct ctttcaacgt
    28921 aatggagggc aaaattttac gccgttcatc tcgcgagaag tacggcactt gcgagagcat
    28981 ctgcgcgggc ataatcgcct gctcaaacgc tttacccttt tctgcggaat ccatcatctg
    29041 gttataccag gacttcgcct gctcaagctg ctcttccgta gcggtatagc taaagtaccc
    29101 ctcgagcaat gcctggaaca gctgcggcag acgctgggtg taaccattag cattaatcat
    29161 aaggccgttg ttagcgttgg tggaaaaact tatgccacca accgacgcct ggttgcttaa
    29221 ctgatcaagc gccagccctg cgagataatc attgagcgca aacatcacct gattgcgggc
    29281 gctgtccatg gctttcggat tacgcaaaat caggctgaca tcagctttgg gctcgctggc
    29341 aaaataacgg cttggcgcat acaccacgcg cagattcgac tcatcaacaa tcagctctgg
    29401 atggtcgtat ttcttctctg acttaatcag cgagaaatca tcaggaatat aagggttaag
    29461 ctctggcaaa gagagcgcaa tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc
    29521 gctgatttta tcgacctgat acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc
    29581 tttcgggctg atataccaga tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt
    29641 tactgcttta gcatcgtacc gatcggcaat attgactgca tccagcgtat gctcaacagg
    29701 aacgcgaatc atggtatctg ccagccattc gacgtaatcc atatcacggg tgatcgacgg
    29761 ataacggaag tcgatatcca gcacattcgc cagttcatcg aagtattgtt tatcaatgcc
    29821 tttttcacgt aacagattga gatagctaaa aattgccgcc acaacctgat cgcgattagc
    29881 caggccttta tcagttaaag acgcagagat cgctaatacg ccgctgttgc cgttgacgat
    29941 aggatcggag ttggcgctaa tgccctcaac taatccctgc ttttgcagcc agtcagaaag
    30001 tgtacctggg ctgcgattgc caatcagata ggtaatcaat tcatcggttt tactacggaa
    30061 cttcgctgag ttgttatcga tgcgaaactc aacgcgcaac actttacgcg gcagcgcagg
    30121 gacgtaatga atgataatgc ccttttgcgc gtcggtgact accggcacgg tgatttccgg
    30181 ttttttgctc tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc
    30241 cggcagcggt ttattactgt aaataaccgc cttcatcaaa ttggcggaat agtacttctc
    30301 gtggaaatct ttcagcgcct gctgcaccgg attaccaggt ttgtcgctta aagtttcgag
    30361 gttaccacca gaaaactttg aaccggggtg tgccgggtta atggtttctg cgctgacctg
    30421 tgccatgcgc atcccgtcac gcgtacgcgc catggttaat tcagcgttca ctgcattacg
    30481 ctcacgttcg gcgtatttct tgtcgagcaa gggttctgca atagcatcgg ccaggcggtc
    30541 taccgcaccg ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc gatacggcgc
    30601 agtgctggca ttgtgactac cgccgtgcat tttgagatat tcggccagac tgtcagcctg
    30661 cgggtacttt ttcgacccca tcagactcat atgttcaagg taatgtgcca gcccctggta
    30721 cgcctcggga tcttccagcg acccaacggg cactaccagc gccgagagcg atttaactgc
    30781 ctgcggatca gaaaccagca agaccaccat accgttatcc agacgtatag cctgatactg
    30841 gcggttatct ttatcacttt tacggatggt ttcctgaatc ggctgccatc ccgtttctgc
    30901 ctgacttaat ggtgcccaga gggcaacaaa caacaataat gctttgaacc aggtgctgcg
    30961 gggcattcac ggacctcata atcaacttta ttttctggac agattcaaca ggttaacgca
    31021 aatcatctgc cagaatttaa tcttgtgctg cacgagtcag cctatgttta tataaccatc
    31081 agtccgtgac tggtgcgcat cataaagtaa gcagatagat tgcgcaattt ttatacaaca
    31141 ctcatgactg attaaagcga aacagcggta acaggaaacg ttgcgactgt tcaacgatgg
    31201 cctccattgt ctctggtgtt aattgccgcc agagccgttg ataccagata tcatcacctt
    31261 cgccacgcac catcatgttg ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca
    31321 acgtggaatc gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt tttagccacg
    31381 cgccgccact ttcaggtaac accagcaatg gcgcggacat tccttcacga tacccctcaa
    31441 tcagttgtga gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa cgccactcac
    31501 cgtctttgcg tagaaaaagg cgactttcgc cattaccacc gctggcacag tagacaaggt
    31561 gttccagcca aagttgcatt ccctgcgcca cacttaataa agagggacgc cagcgcaaca
    31621 ggccatccgg ctgcacctgc ggcaaccagc cagttatctg cacaccgttg caggcgagat
    31681 caatttccat actctgcccc ggctggcgac aggcaatgac tctgtcggca agctgctgca
    31741 tctcctggca ctgtgtttcc cagaaaattt caccaaaagc gccatacggt aaatcccctg
    31801 ccgcccggaa gcggcggaac aagcgttcgg catcatcctg ctcaaccagt gcattcaata
    31861 actgctgatt gatttgataa cggctaagtc cttccagaat aaatggctcg gtgtctggga
    31921 tttcgctgtc ttcagtatgg aagttcacct gcaaacgcat ctggaagaac gcccgcaccg
    31981 gatgtgccca gaatcgttgt agcgtttcca gcggcacggt ttccggtaag gtaaacggca
    32041 gcggctgaac aaattcagaa tgtgctttac cagcctggct ggccgcaggt agccattcac
    32101 gagcatagct ttgtcgttcg cctggctggt agttttgcgg atcaaacggc atccgggtat
    32161 ggaggcaagt aagatgcgct tttacccttg cctcgctttc atcacagttg agcgcttcat
    32221 cgcccggtag ataatgactt tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg
    32281 ggaaacgttc actgttatcc tgaatggaac gcccgatata gctgatatag agtttttgct
    32341 gcgcggaaat taacgcttcc aggaacaggt agcggtcgtc atcgcgacgg ctacggtcgc
    32401 cacgcttcgg tttctggctc atcaggtcaa agcccaatgg cgcaagctga cgtggataaa
    32461 cgccgtcgtt cattcccagc aggcaaacca ctttgaacgg aattgaacgc attggcatca
    32521 gagtacaaat gttaaccggt ccggcgagaa aacgctggct gatacgttct tgatccagac
    32581 gctgtgccag ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta
    32641 aaccttcggc gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt
    32701 ccgcatccgg caggaagaag gcgttgagca tatcgcgaca aaccggcaac cactcttcta
    32761 gcggacgctc ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc agtgaagcca
    32821 gatgccccac cagttctgca attaagccgc tcgattcatc ataaggtaga accgattgcc
    32881 actcgccctg cgcgctctcc atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc
    32941 aggtgtgttg tccggtggcg gggagttcca gctcgcgaac gttgtcgtca tctatccccc
    33001 aacgaatgcc ggattcgttg acccactggc gtaaataacg cagcccttct tcggtgatgt
    33061 caaaccgcgc cgccagcacc ggcacatcca gcagcgccag cacatcctct gacacaaagc
    33121 gactgtcagg cagtgataac aggctgataa acgcttccag taccggatgt gactgccgcg
    33181 cacgacggtc ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta ccaaacactg
    33241 cctgaataaa cggactgtag ctgtcgatat cagccaccat cacgatgatg tcgcgcggag
    33301 taagtgtcgg ggcttcctcc agcatcgcca gcaagcgatc gtgtaaaact tcaacttcac
    33361 gctgcgggct atggcaaacg tggaatgtga tactgctatc cagtggatca agcgggcgtt
    33421 tgttatcgct acgggaaaac tcttcgatgt tcacacccgc aacggcgcgg ttttccagtt
    33481 ccagaatgtc agactgaatg ttatgcagca ggttatctgg cgtcacatcg acaaaagcgt
    33541 ccagctcctg gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc ccaagcttcc
    33601 cccatgaggc cagcagcgag ttgccgacat cctgttcacc atcgctgtta aagagctgtc
    33661 cggcattttc gctatcgcga aatagcggca attcgcgatc ttcaaaactg tgtcgacgct
    33721 gacgagtcag cagtttcgcc agataagcag gatctttaat atcgccccag taataacggc
    33781 aggggttggt aaacaggaga tggatttcaa tatgtttacc cagcgcctgg agcgcctgga
    33841 gataaacagg cggtaacgcg gaaataccgc atataaagac gcgcgaaggt aaccccggcg
    33901 ggcaggtcgt cgcggactcc agcgtttcga taaagcgctg atagagattc gcgcggtgcc
    33961 agcgcggttg tccgagttca tgggtatatt ccaccagcgc cttccacaac ggggcttgcc
    34021 aggcctgtgc ttctcccagc ccttcaacca aatgtcctgt ttcccactgt gccagccagt
    34081 ccggacgata gaccagatac tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca
    34141 gttttcgctt gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct
    34201 ccagcaattg cggcagcaga gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc
    34261 tctctttggg gatttccggt aacacccgga cgaacatatc ccagataaag ctcgctggca
    34321 gcggaaaatc aatgtttgcc gcaataccaa acttttgcga cagggtcatt tgcagccact
    34381 gcgccatacc ggtactttgc accagaatca tctctggttc gaaaggatcg tccagccgtt
    34441 cgcgttcgac aataaactcc atcaacgctt ccagcacgtc cagacgattg gaatggtaga
    34501 cccttaacat agcggctcct gactactgac aattcgggca atgcaggcgg gtcatctcgc
    34561 cttctctgcc cccgggtgaa actagcgtaa cgctgatgct gacacatccc gcctgcgatg
    34621 tctgcattcg gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct
    34681 gccaaccatg ccgccagagc ttttggtact ggtttctgct ggcgagactg ttcattgatg
    34741 ttcgctggat acccgataac gcagtgacaa tcatcaccat cagcaccatc agcaccatca
    34801 gcaccatcag caccatcagc accatcgcaa ccattacctc cggcaggcta aagccttgtt
    34861 gattcttcag ggaagctgac ataatgcccc ctctttcagc ggacaaaaat cgctccagcc
    34921 gcgtggcgaa aaaacaattt ttccatcaat gacttcgcct gttcgccaca acgaaacgcc
    34981 ttcataaccg gcaatcaata aggcttcatt atctgccagt aaacgcaaac aaacctgggc
    35041 atcggttcca gcgtactgcg agcactgaac tgctggctgc gtctgccagg agtgcatttt
    35101 tccccacgcc agcgccgact gaacgatggc ctggcggcgc aatgactgac tttccatgct
    35161 cacgcgagag gcaaaactgc gatcctgctg actcattcct tgcaatagca agctacccaa
    35221 aaccagcagc atcaggacca gagccagtga cgaaacacct ttttcgcggt tcacaggttg
    35281 aatcctgtca cgctatagct ggcatccacc acggtttgcg gttcagactt actggcagca
    35341 cgcatattaa ccgtcaacac cggcgagaag ccgctgacat cctgacgtac gacctgaaaa
    35401 gtgtcgataa tgatggcatc cggattagtg actttatccc agcccttacc ttcacaggat
    35461 gtcgcaccgc gtagcgtttc cagcacatgc tccttcagac gaaatccaat ctggtcggac
    35521 tcttttaccg gttcgcgatc ccagataccg ttactgttcg catcccactg cacaatgata
    35581 cagtcaccct gtccgacaat ttccagccct tcgccggtac agctgccatg acaataaccc
    35641 gccctctgga gatgcttcgc gacggtaaat acccgcagcc agatttcatc ttccagtgcc
    35701 agcttacgag tgctcgttaa actttcacgc tgtaacgcag gcagaaagcg tgcagccccc
    35761 agcaacaata cgctactgat cgccatagca atcaacactt ccagcagaga aaaaccttgc
    35821 tcttttacag gcatccttct gtttctcctt gctgataaag ccggagtctt ccccacggcg
    35881 aaaccaccag ccaccactcg cccgttgagt ttttgaagcg aatatgcacg gcccatgcgg
    35941 tattgcgcag gccaaagaaa gcaagcgaag gtgtcaggtc gctcatttcg acttcgggcc
    36001 agcgtggcac aaagaccaat ggtgaactgc catgacaggt attggcccca gcagcggaac
    36061 tcacaaggca ccataacgtc ccctccctga taacgctgat actgtggtcg cggttatgcc
    36121 agttggcatc ttcacgtaaa tagagcaaat agtcccgcgc ctagctggcg gtttgccaaa
    36181 gccgttgcga ctgctgccag tattgccagc catagagtcc acttgcgctt agcatgacca
    36241 aaatcagcat cgcgaccagc gtttcaatca gcgtataacc acgttgtgtt ttcatgccgg
    36301 cagtatggag cgaggagaaa aaaagacgag ggccagtttc tatttcttcg gcgcatcttc
    36361 cggactattt acgccgttgc aggacgttgc aaaatttcgg gaaggcgtct cgaagaattt
    36421 aacggagggt aaaaaaaccg acgcacactg gcgtcggctc tggcaggatg tttcgtaatt
    36481 agatagccac cggcgcttta acgcccggat gtggatcgta gccttcaatc tcaaagtctt
    36541 cgaaacggta gtcgaagatg gattcgggtt tacgtttgat aatcaacttc ggcagcggac
    36601 gcggttcgcg gcttaattgc agatgagttt gatccatatg gttgctgtac aggtgcgtat
    36661 cgccaccggt ccagacaaaa tcacccactt ccagatcgca ctgctgcgcc atcatatgca
    36721 ccagtaacgc gtagctggca atgttgaacg gcaggccgag gaagacgtca caggagcgct
    36781 gataaagctg gcaagagagt ttgccgtctg ccacatagaa ctggaagaat gcatggcacg
    36841 gtgccagcgc cattttatcc agttcgccta cgttccacgc tgaaacaata atgcggcgcg
    36901 aatccgggtc gtttttcagc tggttcagta ccgtagtaat ctggtcaata tgacgaccat
    36961 ctggcgttgg ccaggcgcgc cactgtttac catacactgg cccgaggtcg ccattttcat
    37021 cggcccattc gtcccagatg gtgacattgt tttcgtgtag ataagcaatg ttagtatcgc
    37081 cctgcaggaa ccacagcagt tcgtggatga tggaacgcag gtggcaacgt ttggttgtca
    37141 ccagcgggaa tccatcctgc aggttaaaac gcatctgatg accaaaaatg gaaagcgttc
    37201 cggttccggt acggtcgttt ttctgtgtgc cttcgtcgag cactttttgc atcagttcta
    37261 aatactgttt catggttcct caggaaacgt gttgctgtgg gctgcgacga tatgcccaga
    37321 ccatcatgat cacacccgcg acaatcatcg ggatggaaag aatttgcccc atgctgatgt
    37381 actgcaccca ggcaccggta aactgcgcgt cgggctggcg gaaaaactca acaatgatgc
    37441 gaaacgcgcc gtagccaatc aggaacaagc ctgagacagc tcccattggg cgcggtttac
    37501 gaatatacag gttgaggata ataaacagca ccacaccttc cagcagcagt tcgtaaagct
    37561 gtgatgggtg gcgcggcagc acaccgtaag tgtcgaaaat ggattgccac tgcgggttgg
    37621 tttgcagcag caaaatatct tctgtacggg agcctgggaa cagcatggca aacgggaagt
    37681 tcgggtcaac gcggccccac aattcaccgt taataaagtt gcccagacgc ccggcaccaa
    37741 gaccaaacgg aatgagtggt gcgataaaat cagagacctg gaagaaggaa cgtttagtac
    37801 ggcgggcgaa gataatcatc accacgataa cgccaatcag gccgccgtgg aaagacatgc
    37861 cgccgtccca gacacggaac agatacagcg gatcggccat aaactgcggg aaattgtaga
    37921 acagaacata accaatacgt cccccgagga agacgccgag gaagcccgca tagagtaagt
    37981 tttcaacttc atttttggtc cagccactgc ccggacgatt cgcccgacgt gttgccagcc
    38041 acattgcaaa aatgaacccc accagataca tcaggccgta ccagtgaagc gccacgggtc
    38101 ctattgagaa aatgaccgga tcaaactccg gaaaatgcag atagctactg gtcatctgtc
    38161 accacaagtt cttgttattt cgctgaaaga gaacagcgat tgaaatgcgc gccgcaggtt
    38221 tcaggcgctc caaaggtgcg aataatagca caaggggacc tggctggttg ccggataccg
    38281 ttaaaagata tgtatatgat ccgcgctata accctccgcg aatcagcccg cccatgccgc
    38341 gacgctccat aaaggctgca acctgatgac gaacttcggt cgccagttgc gcttccagac
    38401 tacgctgcgc aagattttct gcttcggcaa aatcaatgcg ccgcagcagg tattttaccc
    38461 gcgctacaga acgtccgttc atagacagat ggcgataccc aagcccaatg aggattgcca
    38521 cgcacatggg atcgcccgcc atttcaccgc acaaacgaag atcgatacca tgtatttccg
    38581 cttcccgggc gatcatcgcc agagctcgta acattgcagg atgaagactg tcgtaaatgt
    38641 tcgccacccg ggtattgttg cgatcaacgg ccagaatgta ttgagtcaga tcgttggtgc
    38701 caacagagat gaaatcgacc ctctttgcca gatgcggcag cataaatacc attgacggca
    38761 cttccagcat gatgccgata cgtggtttgg gaatttcgta accgatcatc tcctcgactt
    38821 cacgtccggc acgttcaatc aggcggcgtg cttcgtcaac ttcatcgagg ctggtgacca
    38881 tcggcaacag aatattcagg ttgcccgtag cggcattagc acgcagcatc gcccgcacct
    38941 ggatcaagaa gatctccggc tgatcgagcg taatgcgaat cccacgccaa cccaggcatg
    39001 gattctcttc gctgatcggc atgtaaggca gctgcttatc tgctccgaca tccagcgtac
    39061 gcaaggtgac gggtttatca ttgaacattt gcagcatccc ctgatactgc gccacctgtt
    39121 cttcttccga cgggaaacca ctttgcagca tgaatgggat ttcagtgcga taaagaccga
    39181 tgccatcaat acagctgccc agtttttctt catgttccgg gcttaaacca gcattgagca
    39241 tgacttttat acgctcaccg ctttttaact gcgcgggtaa attgacgtca tcttccgcca
    39301 gacggctaag ctcaatctct tcactaatta gccgctgata ttcttgcagc agtaccggct
    39361 ccggatcgac cagcaattca ccgcgatagc catcaacgat cagcgtccta cgatgcagca
    39421 ccgaaggctg aatatccgcg cccatcacgg tagggatccc cagcgcacgt accatgatcg
    39481 cagcatggga gttggctgcg ccatctcgca cgacaacacc gactaagcga tcctggggca
    39541 gctcagcaag agttgtcgct gacagttcat ctgccaccag aatgaaacgt tccggccagg
    39601 cgttcggccc ttgattagcg tcatcaagat gaaacagcaa tcgctgaccc agcgcacgta
    39661 aatcaccagc ccgctctttg agatagttat cgcttagcgc ggcaaactgt tcggcaaatt
    39721 tttcaatgac cgtttttacc gcccactctg ccaccgagcc tttatcaacc tcggcaaaca
    39781 attcgcgacg cagccgggtg tccgaaagca ggtgcgagta aagatcgaaa atagccgccg
    39841 tttctttttg tgctccggcg gcaaagcgtt tgctgtagcg gcgaaactcg tttgccgctt
    39901 cttccagcgc cccggtcagt cgttcgcgtt ccagagccgg atccagcgtt gatgcctgat
    39961 acacctgttc cattaaaggt aacgtggcat cctgccagcc ttcggcaatc gccacaccag
    40021 gtgctgccgg taatgcgcgg attcgcgtct ggcgatattg cccaaacagg gcagtcaact
    40081 gcgactgaga aagaatagct gccatctggg tggcaagcgt caccaggaag gattcttcac
    40141 tttcgtcata ctggcgcaac tctcgttgct gtaccaccag tacaccaagc aactggcgac
    40201 gttgaataat tggtacgcct aaaaacgcgc ggaaacgttc ttcttttacg gaggggatgt
    40261 atttgaagct ggggtgcttt tgcgcatctg caaggtttat cggttccgcc agcctgccaa
    40321 ccaggccgac gatcccttca tcaaacgcga gcgttacagt gcgaccgcgt ggttttttca
    40381 gcccccgggt cgccatcagg tagtaacaac gtcgatcatg atcggccagg tagaccgaac
    40441 agacctcggt atccatcgca agacagatgt cggtaaccag aatatttaac gcctcattca
    40501 ggcgtggtgc gctggctacc ttttcgacta tttcgcgcag gcgagtgagc ataatcggcg
    40561 tgacttaacc tcttttacgt cgataagcag atgcgttttg tggttttggc gtattttcct
    40621 gcagtgacat caccacactc gcgaactctt tcattaccct acggtagaca tcacgtttaa
    40681 atgacaccac ctgtctgacc ggataccagt aacttaccca tcgccagccg tcaaactctg
    40741 gtgtactgct ggtttgcata ttgatttctg catcgccgct taccagctgc aagagaaacc
    40801 atttttgttt ttggccgata caaaccggct tcgtgtccca acgcaccaaa cgtttcggta
    40861 atttgtagcg caaccagtta cgcgttgaag caaggattcg aacgtctttg cggcttaatc
    40921 ctacttcttc aaacaattca cggtacatcg cctgctctgc ggattctccg gggttgattc
    40981 cgccttgcgg aaattgccag gagtgctgac caaatcgccg ggcccacatt acctgcccct
    41041 ggcgattaca aatcacgata cctacgtttg ggcggtagcc atcgtcatca atcaccggac
    41101 tacctcaaaa taaagcttta tatacgaatg attgtttcat actccaggaa gacggtaaac
    41161 cactctctgc agggcattac acactaataa caattgaata actcacagtt atgtgcagag
    41221 ttataaacag aggaaggggt ggatagccgt ttttattcac cttttctgtg gatagagttg
    41281 tgaagaacta cggaattact acgggaaaac ccggagcatt ctgaataagc ccgatataga
    41341 cattttaaaa tattcattat tagtcaatgc attacatgta atttgagtaa tgaaaaagag
    41401 taattcgtga cccaggtcac acctctcatt tacgggttgg cgaaagatca accaatgccg
    41461 tgtttatcca cagaatgtgc cactaagtta agcactgaac cactaaaaac tggagtttcg
    41521 tcgcacgtca aggctgtaaa tgaaaacagt agtggaggtt tttcacagtt atcccagctt
    41581 tctgtggata acatggtgta agatcctgtt tattttcagt gaccagattt ggaaaaccta
    41641 ttgcagtgtt gcgcaactcg attaccggca gcctaaaaag caatataaat cagagaattg
    41701 aacaacacat gtggaaaaag ttacactgcg aatattcggc acataattgc tgtttgtttt
    41761 ttaaccaagg tatcatgaca tgtcccaacc tcgcccactg ctctctcctc ccgaaactga
    41821 agaacagttg ttagcgcaag cacagcaact ttctggttat acattgggag aactggcggc
    41881 acttgacggg ctggttacgc cagagaattt aaaacgcgat aagggctgga ttggcgtgtt
    41941 actggagatc tggctaggtg ccagcgcagg gagtaaacct gagcaagatt ttgctgctct
    42001 gggcgtggaa cttaaaacta tccctgtgga tagtcttggt cgtccgctgg aaacaacatt
    42061 cgtttgtgtt gcccggttaa cgggtaatag cggggtgacc tgggaaacca gccatgtgcg
    42121 ccacaagctc aaacgcgtac tgtggatacc ggttgaaggc gagcgcagca tcccgctggc
    42181 gcagcgtcgc gtaggatcac cgttgctgtg gagcccgaat gaagaggaag accgacagct
    42241 acgcgaagac tgggaagaat taatggatat gattgttctc ggtcaggttg agcggatcac
    42301 cgctcgtcac ggggaatatt tacagataag accgaaagca gcgaatgcga aagcgcttac
    42361 cgaagccatt ggtgcccggg gcgaacggat tctgacgctg ccacgcggct tttatttgaa
    42421 gaagaatttc accagtgcac tactggcccg tcattttctg atccagtagc catcgctttg
    42481 acctgccgct ttccgggcat ataattaccg cttcattttt ttggcagggc tttttagatg
    42541 ttatttgcat ggataaccga tcctaacgct tggcttgcgc tcggtacgct gacgctgctg
    42601 gagatcgttc ttgggatcga caatattatt ttcctttctc tggtggtggc aaagcttccc
    42661 acagcacaac gtgctcatgc gcgccgtctg gggttggcgg gagccatggt tatgcgtctg
    42721 gcgctgctgg catcaatctc ttgggttacg cgcctgacaa atccgctttt tacaatattc
    42781 agtcaggaaa tttctgcccg tgatttgatt ctgcttctgg gtggcttgtt ccttatctgg
    42841 aaagccagca aggaaatcca cgaatccatt gaaggtgaag aagaagggct gaaaacacgc
    42901 gtttcatcat tcctcggcgc tatcgtgcag attatgctgc tggatattat cttcagcctc
    42961 gactcggtga ttaccgctgt gggtctgtca gatcacctgt ttattatgat ggcagccgtg
    43021 gtgattgccg taggcgtgat gatgttcgcc gcgcgctcga ttggtgattt tgtcgaacgc
    43081 catccttcgg taaaaatgct ggcgctctct ttcctgattc tggtgggctt taccctgatt
    43141 ctggaaagtt tcgacatcca cgtaccgaaa ggttacatct acttcgcgat gttcttctct
    43201 attgcggttg aaagcctcaa cttgattcgc aacaaaaaga atccgctctg atactccgta
    43261 cgctctcctg cgggagggcg tactcactcc ttttaccctc acagtttaag attttcctgc
    43321 tttcaagata tataacgtcg gtttataaac agactattat cataggtagg ccaaatgagg
    43381 ataaccggat gaaaaaatgg gcagtaataa tttctgcagt cggactggcg tttgctgttt
    43441 ccgggtgttc cagtgattac gtcatggcga ccaaagatgg ccgtatgatt ttgaccgatg
    43501 gaaaacctga aattgatgat gataccgggc tggtgagtta tcacgatcag caaggtaacg
    43561 cgatgcaaat taaccgtgat gatgtttcgc aaattattga acgttaacaa ataaggtcag
    43621 catccggctg gccttaagat ttttctcttt cccttttccc ttccctctgc catttttata
    43681 ttccttatgt cgtgattata aaaaggaaac ggctatgcaa tatcaccgta taccccacag
    43741 ttcgctggaa gtcagcacgc tggggcttgg cacgatgacg tttggtgaac agaacagcga
    43801 agccgacgcc cacgcacaac tcgactatgc cgtcgctcag ggcattaacc ttatcgacgt
    43861 tgccgaaatg tacccagtac ctccgcgccc cgaaacgcaa gggttaaccg aaacctacgt
    43921 cggcaactgg ctggcgaaac atggcagccg cgaaaagtta attatcgcct ccaaagtgag
    43981 cggaccgtcg cgcaataatg acaagggcat ccgcccggat caggcgctgg atcggaagaa
    44041 tatccgcgaa gcgctgcatg acagcctcaa gcgcctacag actgattacc tcgatcttta
    44101 tcaggtgcac tggccgcagc gcccgaccaa ctgcttcggc aaactcggtt atagctggac
    44161 ggattctgcg cctgcggttt cgctgctgga tacgctggac gcactggcag agtaccaacg
    44221 cgcgggaaaa attcgttata tcggcgtgtc gaacgaaact gcatttggcg taatgcgcta
    44281 cctgcatctg gcggacaaac acgatctgcc gcgtattgtc accattcaga acccttacag
    44341 tctgttaaac cgcagttttg aagtaggtct ggcagaagtc agccagtatg aaggggtcga
    44401 actgctggcc tattcgtgcc tgggtttcgg cacgctgacc gggaaatatc tcaatggtgc
    44461 aaaacccgct ggcgcacgta atacgctctt tagtcggttc acccgctata gcggtgagca
    44521 aacgcaaaaa gccgtcgcgg cgtatgttga tatcgccaga cgtcatggcc ttgatcctgc
    44581 tcagatggcg ctcgcgtttg tacgccgtca accgtttgtt gccagcactc tgctgggcgc
    44641 aaccacgatg gatcagctga aaactaacat cgaaagtttg catctggagt taagcgaaga
    44701 cgtattagct gaaattgaag cggtacatca ggtttatact tatcctgcac cataaagatt
    44761 aacgccggtt aaaaccggcg ttaaatatta atgacggcgc tgccagatcc acagcgccgt
    44821 tattgccagc gcaaatagcg caccgaagcc aatgccaatg ggcacgaccg ggatgcctac
    44881 cattaccgcc agcgagtaaa tgcccagcat caacaacatg gcgctgtttt cgccaaggtt
    44941 ttgtactgca atcgcattcc ccgccccgac gcttttttta ccccgctcct gtagcaacgc
    45001 attgagcgga acgacaaaaa agccccccag cacgccaatc agcatcaaca aggcataggc
    45061 tggcagcagc tcgtgttgca gggagaaaat cggtaccacc acgccaatca aaatcccggc
    45121 tggcatacag cgtgacacgg tttccagcgt aaccagcttc gctgccgcac ctgcgccaac
    45181 cacgatacca atcgctacca tcgcgttgag ataggtcggc gtagcgttat cggtaatgcc
    45241 cagcgccacc ggtacccaca gcaccaacag gaaacgcagc gtgacacccg ctccccagaa
    45301 taaactggtg cccaccagcg aaaaacgcgt ttcaccattg caccatagcg aggtgcaggc
    45361 attcaggaaa ctgcgggtca tgttgatgag attccaggac tgccccggac gcgccgccag
    45421 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
    45481 agcgaccagg acatgccagt cagccagcac accaccggct acggaaccga gtaaaatcgc
    45541 cgctatggca gaagcttcca ttaaaccgtt agcttttact aacttactac ccgtggttaa
    45601 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
    45661 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
    45721 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
    45781 cgccaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
    45841 atagaactgc gctttcagta acgccagagt ggcaaacagt agcgcgttat cgccaaacgc
    45901 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
    45961 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
    46021 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
    46081 cagcaagctc cggcacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
    46141 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
    46201 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
    46261 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
    46321 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
    46381 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
    46441 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
    46501 ggcgtgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
    46561 cgttgataga aacgacaggc gcgcattcgg tcacaccgta gccttcaagg atgcgcaggc
    46621 caaatttatc ctgccaaagc tgtttggtac tttcttgtag tttttctgcg cctgccacca
    46681 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
    46741 tcgaggtacc gaacaacacg gtgcaactgc ggtcatacac caagtccggc acaatgcggt
    46801 aatgcagcgg gcttggataa aggaacactt ctgcacctgt aagcagtggc gtaaacaggc
    46861 ctaccgtcag cccaaaggag tgaaacagcg gtaatgccga cataaagcga tcgttggtgg
    46921 tgaagtcggc aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga
    46981 cgacgccttt tggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg
    47041 gctgctgttt tacctgcgcc aggcgcggca tcagcaaatg agcgaagatc catactttgt
    47101 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
    47161 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
    47221 tgatttcagc cgccgtaata gcactggtca gcccttttac cccggcagtg tagttcatca
    47281 ttgcgggaat gcggcgacgc gcgatggccc caaaaatcac tgccgcactg atgcctgcat
    47341 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
    47401 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
    47461 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagagttt
    47521 cacgcgggcg caccgccata cgcgcttcca tcattatttg atgcagcatt tcgccagcga
    47581 ttttgcgacg gtcacgggca cgcggcgcat ccggcatcgc cacctgcgtt ggaggcaaaa
    47641 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
    47701 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
    47761 caaaacccgc gccatcgtag attttcatca gcgagcctgt agtggtgatg cgtccttcag
    47821 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atagccatag
    47881 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
    47941 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
    48001 aaatgccatc aataaaagag acgtgattag gcgtaattag tacgcgctcg cccttcagtg
    48061 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
    48121 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
    48181 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
    48241 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
    48301 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
    48361 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
    48421 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaaca cgaagttgac
    48481 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
    48541 ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
    48601 ttacttattt gcgtttggct cacattccca cgatgaaaac acgccacccc ttgaaccaac
    48661 gggcgttttc cgtaacactg aaacaatgta agcgtttacc cactaaggta ttttcatggc
    48721 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
    48781 taataattca cccaaagcca gcgaagcttc ccggctggct gtgcatagtg caatggagtc
    48841 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
    48901 cggtctgatc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
    48961 acaggtggct tatcacaccg gtaatttctt attgattggc aacggttacc acaacgaaca
    49021 aaaagagcgt caggctattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
    49081 tgccaaaatg atcccagatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt
    49141 gctgatcaac cgtatcctgc ctggctttga aaaccgttgt attgctcttg acgatcgtta
    49201 cggtgcctgg ctggcaacgc gtcatttaat tcagcaaggt catacccgca ttggttatct
    49261 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
    49321 tgctgaaagt ggtattccgg ccaatgatcg gctggtgaca tttggcgaac cagacgaaag
    49381 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
    49441 ctgttataac gattcaatgg cggcgggcgc gatgggcgtt ctcaatgata atggtattga
    49501 tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
    49561 tccgcgcctg accaccgtgc gttacccaat cgtaacgatg gcgacccagg ctgccgaact
    49621 ggctttggca ctggcggata atcgccctct cccggaaatt actaatgtat ttagtccgac
    49681 gctggtacgt cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
    49741 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
    49801 gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctattgt
    49861 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacctg
    49921 ccttcacttc cggcaaggcg cgggtttcaa catttccccc ttcctgctgg gtaaagacat
    49981 cgcccgattc acataacggc ccggcgacga cggtttccac cgttggcgcg tgttccagag
    50041 aacgaccatc agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca
    50101 tcagatcgtt gaacccggca tcaaccagca caaagtggcg gctacccatt tgtttgacgc
    50161 tccgcacctg agtaattaat acgccagact gcgctaccag gaagcgaccc ggttcaattt
    50221 ccagtttcac agggtggccc aaatggcgag cgatttgctc acgcgcggca ttccacagac
    50281 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac
    50341 cgcccgcaga aatagcctgt aaatcctgac cgaattcgat aacctgacgc accatagcac
    50401 cacacacctg ctccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
    50461 ctagctgcag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
    50521 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
    50581 ccggattaac gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgagcg
    50641 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
    50701 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
    50761 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
    50821 gcattaagcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacttcaa
    50881 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
    50941 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga
    51001 acagtgaatg tggcataaca aactccagat gagtgctttt ttatgattac gccacatcat
    51061 aaaaagaata aaaaatatcg atttatgtcg agtctatgca aaaatgatat ggattaccgg
    51121 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
    51181 taatgaccgc cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg
    51241 tcagccgcga actggcgcgc tttgagaagg tgatcgggct gaaattgttt gagcgcgtac
    51301 gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
    51361 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
    51421 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
    51481 cctttctggc acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
    51541 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc
    51601 ctacgggaac agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg
    51661 gtcatccgct ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaattaca
    51721 tcagcctttc ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc
    51781 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac
    51841 gggcgggggt aggtatttcg gtggttaacc cgctcaccgc actagattat gcggcaagcg
    51901 gtttagtggt gcggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
    51961 tgcaccgccc gtcatcagcg ctggttcagg cgtttagtgg gcatttacaa gcggggttac
    52021 cgaaactggt cacttctctt gacgctattt tgtcgtcagc tacgacagca taaaagcgac
    52081 agcatcctcg gcatggatcg ccgcggtatc aaacacaggc aaaacactgc gctcttctgg
    52141 caccagtaaa ccaatttctg tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag
    52201 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
    52261 ttcttcaaaa ataatctgat taattttcgc ccgttcatcc gcttcaggaa taagacaatt
    52321 gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
    52381 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
    52441 aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca tccgccactt tatgcatcgt
    52501 attggtacat aacacaatac cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc
    52561 cagaatgtcc ccggttttat cccattcccc gcgacgctgg cactcttcta tttcatgaaa
    52621 atcgacgcta tgtagcagca tttgcgcaga gtgaagccca ccaagccgct gtttaatgcc
    52681 ttcatttatc agacgatagt aaggaatggt ggattcccag ctcattcctc ccagcaaacc
    52741 aattgttttc attcctctct cctgatgtgt gttaccgcaa caaggctgaa taaggaacga
    52801 gacaaacgcc tcaacggcca ggtgcccaat ctctattaac gaaaaaaggg ccggatgtac
    52861 agcacatccg gcccgtgaaa tcagacgccg atatttctca acttctcgcc tgccatcagt
    52921 ttgcgttcga tatgttccag cgtgacattt ttggtttccg gaatgagcca gaaagtaata
    52981 cccacaaacg caatgttcag cgcagtgtag agccagaacg taccggcagc gccaatgcta
    53041 tcaagcagtg tcaggaaggc cgcgccgata atcatattcg acacccagtt tgtcgtcgtc
    53101 gaacaggtaa taccgaaatc gcggcatttc agcggctgaa tttcagagca caggatccac
    53161 accactggcg cggcgctcat cgcataaccg gcaatacaca tcatcgtcat gccaacagag
    53221 agccaggaca agccactgga agccgtaccg ttatcaaact gcatcaggca atagcccagc
    53281 accagagtgc ctaacgccat cacgctgaaa ccaattttca gagccggttt acgacctgct
    53341 ttatctaccg taaagaccgc aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga
    53401 gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca ttttgaagat acgcggcgcg
    53461 tagtacatga tgatgttcat accggtaaac tgctgcatcg cctgcaacaa cataccgaga
    53521 aacacagcac gacggacgtt acggttgatc ttaaacagtg cccaaccgcc ctgttttaac
    53581 ttcaggcttt cacgaatttc gttgagttct tctcgcgctt tttccgacgt atcgcgcagc
    53641 atacgcaaca cttcttccgc ctcaatatga cgcccctttt ccgccagcca gcgcgggcta
    53701 tttggcagga agaccaccag aataatcagc agaactgctg gtaaagcaag aacccccaac
    53761 attgcgcgcc agttaccgct ataactgaac gctgtatcgg ataaaaacgc cagcacgatg
    53821 ccgagtgtga ccatcaactg gtacatactg atcatcttac cgcgaacgtt ttcacttgcc
    53881 atttcagaaa gatacagagg agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc
    53941 acacgagcgg cgattaacat ctctacgctg gtcgcaaaag cggaccctat agaaccgagt
    54001 acaaacagga tggcccccgc catcaggctg tatttacgcc ccaggcggaa cgacagccaa
    54061 ccattaaaca gcgcaccaat tgctgcgccg agcatcatgc tactaaccac ccattcctgc
    54121 aaacgactgg tcagcacaaa gtgatcggta atgaacggca acgctccggc gattacgccg
    54181 atatcaagac caaataacaa tcctgcgacc gcagcagcta ccgaaacaaa catattcata
    54241 cgccgcgtat cacgcaaaga acgtggcgtt aaagcagatt ccgtattgat agtaaccatt
    54301 ttttcctgcc agcagagagt aagacattgt gaaaaaatac gtgaacaatt cacacaggtg
    54361 tcaggtcgga aacagcataa atatggatta aattgctgcg
//
LOCUS       DAZOYF010000009        52599 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZOYF010000009 DAZOYF010000000
VERSION     DAZOYF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.009"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..356
                     /locus_tag="ACHUOF_000536"
     CDS             <1..356
                     /locus_tag="ACHUOF_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR0609856.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="ACHUOF_000537"
     CDS             complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="ACHUOF_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR0609857.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="ACHUOF_000538"
     CDS             complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="ACHUOF_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR0609858.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3111..3263
                     /gene="mokC"
                     /locus_tag="ACHUOF_000539"
     CDS             3111..3263
                     /gene="mokC"
                     /locus_tag="ACHUOF_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR0609859.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="ACHUOF_000540"
     CDS             complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="ACHUOF_000540"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR0609860.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="ACHUOF_000541"
     CDS             complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="ACHUOF_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR0609861.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6879..7283
                     /locus_tag="ACHUOF_000542"
     CDS             6879..7283
                     /locus_tag="ACHUOF_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR0609862.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7309..8022
                     /gene="msyB"
                     /locus_tag="ACHUOF_000543"
     CDS             7309..8022
                     /gene="msyB"
                     /locus_tag="ACHUOF_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR0609863.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8171..8737
                     /gene="satP"
                     /locus_tag="ACHUOF_000544"
     CDS             8171..8737
                     /gene="satP"
                     /locus_tag="ACHUOF_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR0609864.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8772..9359)
                     /gene="mog"
                     /locus_tag="ACHUOF_000545"
     CDS             complement(8772..9359)
                     /gene="mog"
                     /locus_tag="ACHUOF_000545"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR0609865.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9474..10427)
                     /gene="tal"
                     /locus_tag="ACHUOF_000546"
     CDS             complement(9474..10427)
                     /gene="tal"
                     /locus_tag="ACHUOF_000546"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0609866.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10706..12136
                     /locus_tag="ACHUOF_000547"
                     /pseudo
     CDS             10706..12136
                     /locus_tag="ACHUOF_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            12206..12982
                     /gene="yaaA"
                     /locus_tag="ACHUOF_000548"
     CDS             12206..12982
                     /gene="yaaA"
                     /locus_tag="ACHUOF_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR0609867.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13135..13431)
                     /locus_tag="ACHUOF_000549"
     CDS             complement(13135..13431)
                     /locus_tag="ACHUOF_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR0609868.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13645..14931)
                     /gene="thrC"
                     /locus_tag="ACHUOF_000550"
     CDS             complement(13645..14931)
                     /gene="thrC"
                     /locus_tag="ACHUOF_000550"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR0609869.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14932..15864)
                     /gene="thrB"
                     /locus_tag="ACHUOF_000551"
     CDS             complement(14932..15864)
                     /gene="thrB"
                     /locus_tag="ACHUOF_000551"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR0609870.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15866..18328)
                     /gene="thrA"
                     /locus_tag="ACHUOF_000552"
     CDS             complement(15866..18328)
                     /gene="thrA"
                     /locus_tag="ACHUOF_000552"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR0609871.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18354..18473)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18409..18474)
                     /gene="thrL"
                     /locus_tag="ACHUOF_000553"
     CDS             complement(18409..18474)
                     /gene="thrL"
                     /locus_tag="ACHUOF_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR0609872.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18688..19374)
                     /locus_tag="ACHUOF_000554"
     CDS             complement(18688..19374)
                     /locus_tag="ACHUOF_000554"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0609873.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19774..19914)
                     /gene="yjjY"
                     /locus_tag="ACHUOF_000555"
     CDS             complement(19774..19914)
                     /gene="yjjY"
                     /locus_tag="ACHUOF_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR0609874.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20010..20726
                     /gene="arcA"
                     /locus_tag="ACHUOF_000556"
     CDS             20010..20726
                     /gene="arcA"
                     /locus_tag="ACHUOF_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR0609875.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20785..22137)
                     /gene="creD"
                     /locus_tag="ACHUOF_000557"
     CDS             complement(20785..22137)
                     /gene="creD"
                     /locus_tag="ACHUOF_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR0609876.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22195..23619)
                     /gene="creC"
                     /locus_tag="ACHUOF_000558"
     CDS             complement(22195..23619)
                     /gene="creC"
                     /locus_tag="ACHUOF_000558"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR0609877.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23619..24308)
                     /gene="creB"
                     /locus_tag="ACHUOF_000559"
     CDS             complement(23619..24308)
                     /gene="creB"
                     /locus_tag="ACHUOF_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR0609878.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24321..24794)
                     /gene="creA"
                     /locus_tag="ACHUOF_000560"
     CDS             complement(24321..24794)
                     /gene="creA"
                     /locus_tag="ACHUOF_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR0609879.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25005..25874
                     /gene="robA"
                     /locus_tag="ACHUOF_000561"
     CDS             25005..25874
                     /gene="robA"
                     /locus_tag="ACHUOF_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR0609880.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25871..26518)
                     /gene="gpmB"
                     /locus_tag="ACHUOF_000562"
     CDS             complement(25871..26518)
                     /gene="gpmB"
                     /locus_tag="ACHUOF_000562"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR0609881.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26570..27090
                     /gene="yjjX"
                     /locus_tag="ACHUOF_000563"
                     /pseudo
     CDS             26570..27090
                     /gene="yjjX"
                     /locus_tag="ACHUOF_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27175..27501)
                     /gene="trpR"
                     /locus_tag="ACHUOF_000564"
     CDS             complement(27175..27501)
                     /gene="trpR"
                     /locus_tag="ACHUOF_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR0609882.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27591..29528)
                     /gene="sltY"
                     /locus_tag="ACHUOF_000565"
     CDS             complement(27591..29528)
                     /gene="sltY"
                     /locus_tag="ACHUOF_000565"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR0609883.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29739..31406
                     /gene="ettA"
                     /locus_tag="ACHUOF_000566"
     CDS             29739..31406
                     /gene="ettA"
                     /locus_tag="ACHUOF_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR0609884.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31513..31680)
                     /gene="nadS"
                     /locus_tag="ACHUOF_000567"
     CDS             complement(31513..31680)
                     /gene="nadS"
                     /locus_tag="ACHUOF_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFR0609885.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31714..32946)
                     /gene="nadR"
                     /locus_tag="ACHUOF_000568"
     CDS             complement(31714..32946)
                     /gene="nadR"
                     /locus_tag="ACHUOF_000568"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR0609886.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32967..34349)
                     /gene="radA"
                     /locus_tag="ACHUOF_000569"
     CDS             complement(32967..34349)
                     /gene="radA"
                     /locus_tag="ACHUOF_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR0609887.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34398..35366)
                     /gene="serB"
                     /locus_tag="ACHUOF_000570"
     CDS             complement(34398..35366)
                     /gene="serB"
                     /locus_tag="ACHUOF_000570"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR0609888.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35472..36116
                     /locus_tag="ACHUOF_000571"
     CDS             35472..36116
                     /locus_tag="ACHUOF_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR0609889.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36144..37159
                     /gene="lplA"
                     /locus_tag="ACHUOF_000572"
                     /pseudo
     CDS             36144..37159
                     /gene="lplA"
                     /locus_tag="ACHUOF_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37191..37454
                     /locus_tag="ACHUOF_000573"
     CDS             37191..37454
                     /locus_tag="ACHUOF_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0609890.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37615..38334)
                     /gene="deoD"
                     /locus_tag="ACHUOF_000574"
     CDS             complement(37615..38334)
                     /gene="deoD"
                     /locus_tag="ACHUOF_000574"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR0609891.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38391..39614)
                     /gene="deoB"
                     /locus_tag="ACHUOF_000575"
     CDS             complement(38391..39614)
                     /gene="deoB"
                     /locus_tag="ACHUOF_000575"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR0609892.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39666..40988)
                     /gene="deoA"
                     /locus_tag="ACHUOF_000576"
     CDS             complement(39666..40988)
                     /gene="deoA"
                     /locus_tag="ACHUOF_000576"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR0609893.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41115..41894)
                     /gene="deoC"
                     /locus_tag="ACHUOF_000577"
     CDS             complement(41115..41894)
                     /gene="deoC"
                     /locus_tag="ACHUOF_000577"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR0609894.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42152..43693
                     /locus_tag="ACHUOF_000578"
     CDS             42152..43693
                     /locus_tag="ACHUOF_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR0609895.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43665..44528
                     /locus_tag="ACHUOF_000579"
     CDS             43665..44528
                     /locus_tag="ACHUOF_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR0609896.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44742..45524)
                     /locus_tag="ACHUOF_000580"
     CDS             complement(44742..45524)
                     /locus_tag="ACHUOF_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0609897.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45521..46594)
                     /locus_tag="ACHUOF_000581"
     CDS             complement(45521..46594)
                     /locus_tag="ACHUOF_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR0609898.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46716..46877)
                     /locus_tag="ACHUOF_000582"
     CDS             complement(46716..46877)
                     /locus_tag="ACHUOF_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR0609899.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46994..47599)
                     /gene="osmY"
                     /locus_tag="ACHUOF_000583"
     CDS             complement(46994..47599)
                     /gene="osmY"
                     /locus_tag="ACHUOF_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR0609900.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47992..49581)
                     /gene="prfC"
                     /locus_tag="ACHUOF_000584"
     CDS             complement(47992..49581)
                     /gene="prfC"
                     /locus_tag="ACHUOF_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR0609901.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49672..50349)
                     /gene="yjjG"
                     /locus_tag="ACHUOF_000585"
     CDS             complement(49672..50349)
                     /gene="yjjG"
                     /locus_tag="ACHUOF_000585"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR0609902.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50364..50810)
                     /gene="rimI"
                     /locus_tag="ACHUOF_000586"
     CDS             complement(50364..50810)
                     /gene="rimI"
                     /locus_tag="ACHUOF_000586"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0609903.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50779..51192)
                     /gene="holD"
                     /locus_tag="ACHUOF_000587"
     CDS             complement(50779..51192)
                     /gene="holD"
                     /locus_tag="ACHUOF_000587"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR0609904.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51295..52326
                     /gene="rsmC"
                     /locus_tag="ACHUOF_000588"
     CDS             51295..52326
                     /gene="rsmC"
                     /locus_tag="ACHUOF_000588"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR0609905.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12527 a        14234 c        13181 g        12657 t
ORIGIN      
        1 attatcagtt tcatcatata agatttgact gcatccaggg cgagcaaggt gaattagttt
       61 ttgaacccag tgacgttgaa tattactttg aaccagtgac gatacaggag tcaggaacca
      121 gcatattaaa aaatgagtta gaaaacagtg aggacggtgc gggtcaaata ggctttcaac
      181 tctccaatga tggtacgaat gaaatccagt atggtaaaag caattactac tcttttcagc
      241 atccccatga aggaagtaat cagattcctc tatttattcg cccgcgtaca tatggaaaca
      301 atgtctcaag cgggcaaata atgagccgag tcaaaatcgt ggtaatgtac aactaatcta
      361 tctcccccac cttaactttg ggggagctgc cgattaacgc accgctggac taaaaagcgc
      421 agaataatcc gtattgcaga ttcgctgtac cgccgggtgc tgaatcatcc gctcagcaaa
      481 aatagcatgg tactcttcca tcacattctc gacgcgacca atttctacga cagttttatc
      541 ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag attgcattgt gcatcgcacc
      601 aaaagctttc atcaaagcgg catcatcaaa ctcgccgagg atttctacgt ttaatccctg
      661 ggagttaaac cagttaagca atttgcgccc taacattgaa cgtcgcccag gaatcaaaag
      721 tcgccgttct tccagacaag ccgggaacgg tttttctggt ggtggatttg tacaccagaa
      781 actcacgcca cattcgccaa ttctcacgga gaacaggcct tcctgctgcg tagagtctat
      841 cggacagtca gaaatgatca tatccagttt atgctgactt aattgctcca gcagcatttc
      901 gtgggtggat tcgaagcagc gaagatgaat gggctcgcct tctaccactg cggcgttaag
      961 tacgctactg accaggcgtt tggaaagtgc atcagccacg ccaacgtcaa acaataaatt
     1021 ggattctttg cgatagttca caatatccag catttcctgg cttaaggtga acattttatc
     1081 ggcatagcga tagaccagtt ctcccagctc gctgggttcg agaccacgtc ccttgcgttt
     1141 aaataatttg ccttgcaggc gctcttccag cgctcgaatc tgtccggtaa tggtttgtgg
     1201 agttaaataa agcgcctccg ctgcgccaac cacggaacct tctttataga catgccagaa
     1261 gtaatacaag tggttgtaat tgatatgaga catgctcatt tctctccctg ataacaatga
     1321 aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc aaactgatgg acgcaaacga
     1381 acgcgtaacc agctgtatcc aattaccgcc gaagagatag aaccgaccag gataccgagt
     1441 ttcgcccagt taatcagttc tggatctacg ctaccaaagg ccaggctggc aataaagata
     1501 gacatagtaa aaccgatacc gcacaggatc cccaccacca taatttgctg ataagtcgtt
     1561 ccctcaggca gatgcgccag tttcaaacgc agcgccaacc agcagaacag actaatcccc
     1621 agcggtttgc caatcagcaa gccagcgatg atccccaatg gcagaatgga ggtcaagcca
     1681 tccagcgtga cgccttgcag tgaaacgcca gcattagcaa atgcaaacag cggcaaaatc
     1741 agatacgcca cccacgggtg caacacatgc tccagtcgct tcgctggaga acgcccatgc
     1801 ttctctttca aaggaataaa gaagccgaca attacccccg ccagagttgc gtgaaccccc
     1861 gatttcaaca ccgcagtcca caacaccacg ccaacaagaa tatagacgcc cgtgcggcgt
     1921 acaccacaca gattcaatac cgcgagtacc gcaattgcta cagccgcgac gccaagggag
     1981 gccatcgata agtcattagt gtagaacaat gcgatgataa tgatggcccc aagatcgtcg
     2041 ataatagcca gagccatcaa aaagatcttc agcactaacg gaacacgact tcccaacagc
     2101 gccagtacac caagtgcaaa agcaatgtca gtagccgccg ggatcgccca cccttcgcgg
     2161 gtaatcggat cggcatagtt aaaagccaga tagagtaatg ccggcacaat catcccacca
     2221 atagcggcga taactggaaa tgcggcctgg cgtaagctgg ctagcgatcc ttgcatcagt
     2281 tcacgtttaa cttccagacc gactaacagg aaaaataccg ccatcagcgc atcatttatc
     2341 cataacagca tgtttttgtt gatttcgagt gaaccaaccc ggagctgaac cggcgtctcc
     2401 agaaagtcgt gataccatcc actggttgcg ccgctgttgg ccataatcat cgccaggata
     2461 gcggcaatga taagaataat gcctcccgag gcatcactgc taaagaatcg atgcagatgt
     2521 ttcacttttt atttctcttt caggtgaata gatcgttttt actattttac cccgcacgaa
     2581 tcatcgttaa aatcagatta tagtgtagga ataattcgtt ttttacgagc cattgaagaa
     2641 tttgcgccat cagcggtgag atgcgttttt gacggctgta agcgctcacg agtgacccta
     2701 agcccagata atcatagctt aaggtgagga ttcctgaggg attaattagg aaaagtaata
     2761 attaatttgt tgtcagtcgg cgatgcttgt taggccaata tttcaaaaat aatccagctt
     2821 gtcgtcaata attgcgatga agagataaat taaagttaaa taattaaatc attaaaaaat
     2881 tacgccacaa aaatgcgagg cgtcttcagg attagaatcg ataaaacaaa atgggttaaa
     2941 gagagcgata taatagcggc gggtgcttga ggctgtctgt ctcaggcatt agctgaacgg
     3001 cagatagaga aaagccccga gtgatatttt accatcaacc cgaggcctcc tatatgctga
     3061 acacatgtag agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc
     3121 ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg
     3181 taacgagaaa agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt
     3241 tcacggctta cgaatccgag taagagcaac ggcggggagt gatccccgcc actttacagg
     3301 tgctcgcata tcttcaacgc accctatttt tacccaggcc tgcccacggg caggcttttg
     3361 gggaggttag cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga
     3421 gcgcgggctg ttgtgctcgc cggttgggcc accgaagctt tcttgcagct cttgcagcag
     3481 ctgtttctgc ttttcgttca ggcctaccgg tgtttcgacg acaacgcggc acagcaaatc
     3541 accctgtgcg ccaccgcgga cagacttgac gcctttaccg cgcatacgga atagcttacc
     3601 ggtctgggtt tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat
     3661 ttcgccaccc agcgccgcca tagcgaagtt gatcgggact tcgcaataca ggttgttgcc
     3721 ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg
     3781 tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac
     3841 ccctgccggg attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca
     3901 tttgttgcac ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg
     3961 tacagcgaag aatccctggc gcatctgcac ctgaccagaa ccatgacagg tcggacaggt
     4021 ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt
     4081 cggaatgcgg atctctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt
     4141 atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc
     4201 gccgaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac caccgccgcc
     4261 catgcctcct tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga
     4321 gtcggtcaga acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc
     4381 accctggtta cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttctgatttc
     4441 acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt
     4501 ttttaaattg cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca
     4561 gtataattac ccgtttatag ggcgattatt ttttgtcttt gacttcttca aattcagcgt
     4621 cgacaacatc gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct
     4681 gggcatgttg ctgctgggcg atttccatca gtttctggga aacctgtgcc agttcctgca
     4741 ttttcgcttc gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagcg
     4801 cagactcgat agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct
     4861 tacgggtgct gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact
     4921 tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt
     4981 tcagaccaga agaagccttg atggtgatct tctgctcttt accgctgttt ttatctttcg
     5041 cggaaacgtg caggataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc
     5101 cgcgcggtgc cgggttgata ccatctaggt tgaactgacc cagagatttg ttatcagccg
     5161 cacgtttacg ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcgg
     5221 tagagaacac ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc agcgtcgtca
     5281 tcacaccgcc catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt
     5341 cttttacgtc accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt
     5401 ccgggttaac gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca
     5461 ttggcatacg agtctgacca ccaacgagga taacgtcgtc gatatcagat acggacaggc
     5521 cagcatcctg cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca
     5581 ggctttccag tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcgtctg
     5641 cagtgatgta tggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt
     5701 tttctgccgc ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct
     5761 gatctttctt gaattcttca accagatagt tgatcagacg gctgtcgaag tcttcaccac
     5821 ccaggtgggt atcaccgttg gttgccagaa cttcgaaggt tttttcgccg tcaacttcgt
     5881 cgatttcgat aatagaaata tcgaaagtac caccccccag gtcataaacc gcgatagtac
     5941 ggttgccagt gcctttgtcc agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga
     6001 tacgttttac ttccagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat
     6061 cgttaaagta tgccggtacg gtgataacag cttcagttac cggttcaccc aggtaatctt
     6121 cagcggtttt cttcattttt ttcagcactt cagcagaaat ctgcggcggt gccattttct
     6181 ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga
     6241 tggaaacatc acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg
     6301 caaacagggt gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag
     6361 tttcaccatc ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct tcggcgttct
     6421 ccagcacgcg aggaatggtg ccatccataa tcgctacaca agagttggta gtacccaggt
     6481 cgataccaat tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt
     6541 gtgagtctgt aataggggcg aaacgtctgg tttcaactgc ccaatttcat ttttttgcag
     6601 actcactcac tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca agggggagat
     6661 gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata tggcaggaca agacgtaaaa
     6721 ttgacagcaa ttttattcat ataaagaatg aatcgcagcc aaggcgttca tcaaaaaatt
     6781 gtaataaaaa gaaaagatta cgtgcctgaa tcttctcttt atcagcagta aactagtggg
     6841 tattcatccc cctacctctt cccactaaga gaatccttat gaaatccgtt tttacgattt
     6901 ccgccagcct ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaac
     6961 tcctcggcgt cattgcaatg ccgcgtaacg aaaccaacga tctggcgctg aaacttcctg
     7021 tttgtcgcat tgtgaaacgc atacaactct ctgccgacca tggcgattta cagttaagtg
     7081 gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca gagcctgaat attccttcag
     7141 aaataaaaga agggcaaacc actgactgga tcaacattaa cagcgataac gacaataaac
     7201 gctgcgtctc aaaaatcacc ttttccggtc atacggtgaa ctcatcggat atggccacgc
     7261 tgaaaattat cggcgacgac taacgccaga atatagaagc cacaaaaaat gaatgttaat
     7321 tacctgaatg attcagatct ggattttctt cagcattgta gtgaggaaca gttggcaaat
     7381 ttcgcccgat tgctcaccca taatgaaaaa ggcaaaactc gcctctccag catactgatg
     7441 cgcaacgaac tgtttaaatc gatggaaggg catcccgagc aacatcgccg caactggcag
     7501 ctgattgccg gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga
     7561 cacggtaaat tgtatcgggc cattttgctc gatgtttcaa agcgattgaa gctgaaagcc
     7621 gacaaagaga tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat
     7681 acctggaaga aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg
     7741 gtgaatgagc tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt
     7801 gtgtcgcatt tgctttccag ccaactgacc cgcattttac gcacccacgc agcaatgagc
     7861 gtacttgggc atggtttgct gcgcggcgcg gggctgggag gccctgtggg cgcagcatta
     7921 aatggggtta aagcggtcag cggcagcacc tatcgcgtga cgattccagc cgtactgcaa
     7981 atcgcctgcc tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa
     8041 attttcattt tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc
     8101 gccctgaaaa cactacagtt attcagggaa attatttcac cattcattcg atgatgattt
     8161 ttgaggaatt atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt
     8221 cggcatgacc accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat
     8281 tattcttgcc atgggcattt tctacggcgg catcgcgcaa atttttgctg gtctgctgga
     8341 gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct
     8401 gacgctggtt gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca
     8461 gttccttggt gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac
     8521 tctgaaaggc gcgcgcgtcc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct
     8581 gctggcgatc ggtaacattg ccggtaatgc cgcgatcatc cactttgccg gctggattgg
     8641 tctgatctgc ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt
     8701 tggtcgcacc gttctgccga ttggtgaatc ccactaattg tcttgagatc cccccgctcg
     8761 gggggatttt tttattcgct aacgtcgcgt cttgcactct tcggtctgaa tgcagcaacc
     8821 acttctggtg ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg
     8881 gcaaaaatac catgcaccac aacgttaccc tcagcgtcct tcacaccttc cagcgtctct
     8941 ttaatagact tcggctgacc gggtaagtta aggatcagcg cctgtttgcg aatcacgccc
     9001 acctgacgcg aaaggatcgc agttggtaca aaatgcaggc tgatctggcg catctgttca
     9061 ccaaagccag gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga
     9121 cgcgccgggc cagttccgcc cgtggtgagc accagatggc aactcatttc atccaccagc
     9181 tcacacaacg tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt ttccagttca
     9241 aacggcgtgg ttagcgccga tgtcagccat tcttccagcg ctggaatgcc tttatcctga
     9301 taaacgccgc tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc
     9361 aagatttttc cgtttaaaca atctcgttaa acagaatgat acactgcgaa gggagtgaca
     9421 gacaggcttc agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc
     9481 agatcgccga tcattttttc cagtttttcc tggtcaatag caaacttacg gataccttcc
     9541 gccagtttat ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg
     9601 atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc gatagcccct
     9661 tcgctctccg ccagctcttt cagcagtgcc ggtgcgatgg tcagacggtc acagcctgcc
     9721 agttccagaa tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg
     9781 tgctctttgt agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga
     9841 gcgtactctt tcttatcggt attcgctttg taccagtcaa gaatacggcc aacaaacggc
     9901 gagatcagga acacgcccgc ctccgcacaa gcacgagcct gagcgaagga gaacagcagg
     9961 gtcaggttac agttgatacc ttctttttcc agctgttctg cagcacggat accctgccag
    10021 gtagaagcca gtttgatcag aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg
    10081 atcaggcgtt ttgctttcgc aattgacgct tcagtgtcat aggaaagacg cgcatcaact
    10141 tcagttgaga tacggcccgg aaccagtttc aggatttcca gaccaatatt tactgccagt
    10201 ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg
    10261 acagcatcat caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa
    10321 gggttggttg tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg
    10381 gccactacgg tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc
    10441 tttaaacagc ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt
    10501 cagcgcaacc gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact
    10561 gcgttgatat cgcccaccat ttttatacaa aacctcatgt atgctacgca gaagttatca
    10621 agtacctcgt agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc
    10681 gcgaccggca ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct
    10741 ttggggatcg gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac
    10801 cggatttgtg cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca
    10861 tccacagcca ggcggtttaa cctcatttca gtcattgtgt accagtcttg cggcgcgcgt
    10921 gggtagcggc aacctggccg gcgttgcgct ggcaattacc gccggtggac caggtgccgt
    10981 cttttggatg tgggttgctg cgtttatcgg catggcgacc tcgtttgccg aatgttccct
    11041 tgcacaactt tataaagaac gtgacgtcaa tgggcagttt cgtggcggac cggcatggta
    11101 tatggcgcgc gggctgggga tgcgctggat gggcgttctg ttcgccgtct ttttgctcat
    11161 cgcctacggc ataattttca gcggagttca ggcgaacgcc gttgcccgag ccctgagttt
    11221 ttcttttgat tttcctccgc tggtgacggg cattattctc gctgtctttg ctctgctggc
    11281 aatcactcgt ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc
    11341 gataatctgg gtactgacca gcctggtgat ttgcgtaata aatatcgggc aacttcccca
    11401 cgtcatttgg tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc
    11461 gggatatacc ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga
    11521 ggtgggaatg ggatcgacgc caaacgcggc agcggcagct gcatcctggc ctccgcatcc
    11581 ggcagcacaa gggattgtcc agatgattgg catttttatc gacaccctgg tcatctgtac
    11641 ggcaagcgcc atgctgatat tactggcggg taacggcaca acctacatgc cgctggaagg
    11701 tattcagctt atccagaagg cgatgcgggt gctaatgggt tcctagggtg ctgaatttgt
    11761 taccctcgtg gttattctgt ttgccttcag ctccatcgtt gccaactaca tttatgccga
    11821 aaacaatctc ttctttttac gcctgaacaa ccctaaagcg atctggtgtt tgcggatctg
    11881 caccttcgca acggtcatcg gcggcacctt gctaagcctt ccgctgatgt ggcaactggc
    11941 agatatcata atggcctgca tggctattac caatttgacc gccattttac tgctctcgcc
    12001 tgtggttcat accattgcca gtgattatct acgccagcgt aaactcggcg tgcgcccggt
    12061 gtttgatccg ttgcgttatc cggatatcgg tcgccagctt tctccggacg cgtgggatga
    12121 tgtttcgcag gagtaatcac aactatcgat caactcattc tctttttttg ctaaagtcgg
    12181 cataaatttc ctgcaaggac tggatatgct gattcttatt tcacctgcga aaacgcttga
    12241 ttaccaaagc ccgttgacca ccacgcgcta tacgctgccg gagctgttag acaattccca
    12301 gcagttgatc catgaagcgc ggaaactgac gcctccgcag attagcacgc tgatgcgcat
    12361 cagcgacaaa ctggcgggta tcaacgccgc tcgctttcat gactggcagc cagatttcac
    12421 gccggcgaat gcccgccagg cgattctggc gtttaaaggt gatgtctaca ccggcttgca
    12481 ggccgaaacc ttcagcgaag acgatttcga ttttgcccaa cagcatttgc gaatgctttc
    12541 cggcttgtat ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc tggaaatggg
    12601 gatccgtctt gagaatgccc gagggaaaga tctgtatcaa ttctggggag atatcatcac
    12661 caacaagctg aacgaggcgc tcgcagcaca aggcgataat gtggtgatta atctggcgtc
    12721 agatgagtat tttaaatcgg tgaagccgaa gaaattgaat gccgagatta tcaagccggt
    12781 gttcctcgat gagaagaacg gcaaatttaa gatcatcagc ttctacgcta agaaagcacg
    12841 cggtctgatg agtcgtttca ttattgaaaa tcggctgacc aaaccagagc aactgactgg
    12901 ttttaatagc gaaggttact tctttgatga agattcctcc agcaatggcg aactggtgtt
    12961 taaacgctac gagcagcgtt aagtttgtag tcaatgccgg atgcggcgtg aacgccttat
    13021 ccggcctaca aaagcatgca gattcaatat attgcagaat taacgtaggc ctgataagac
    13081 gcgccagcgt cgcatcaggc gctgaatgcc ggattgttac ccggcatttg tcatttagcg
    13141 gtgatgtttg cctggaccat gaccgccgtg atgatcatga ggagctttct tatggtggcg
    13201 cggcggtggc ggcggtccgt gtgggtgcca gcgattgcct cgccattcat aatgttgttt
    13261 ccaccagccg tggtcgcgcc agtgacctcc atcccagtaa tagccacgat tatcacgatc
    13321 gcctatctgt aattttactg acgggactaa cgtaatttcc gcagcctgtg ctgccatggg
    13381 agcgaccaga accagggaaa gtgcgagtac gatagattgc atctttttca caggttactc
    13441 cttttatcat tgcccgttat gggtcgatat ccggagatta cgcgatgaga acgacggttg
    13501 ttattctccg caatcctaat ctctaagtta ccgcatttat tgataatttc tcctttttcc
    13561 ctgtcgtttt tctccataat ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg
    13621 cctgattgag ataatgaata gattttactg atgattcatc atcaatttac gcaacgcaga
    13681 aaaatcggcg ggcagattat gtgaaagcaa gggtaaatca gcacgttctg ccagctcttt
    13741 tggcagatcc aacgtttcac cgagaatcgc ttccacgctc tctttaaatt tcgccggatg
    13801 cgcggtgccg aggaacaagc catattcgcc tggattcaac tggtcacgca gcgcacgata
    13861 cgctacggca gcgtgcggct ccgaggtgta gcccagttct tttaactcac gcattgtctg
    13921 ttgcgtggtt tcatcatcca cggctgcata acccagctct ttcagttgcc agattttgcg
    13981 gcggaacaac tcttccacac gcggccagtt gttcggctga ctcacgtcca tcgcgttgga
    14041 taacgtcgcc tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac gtggcacggt
    14101 atcgttcacg ttggtcgcag caataaaacg tttcaccggc agaccgaatg acttcgccag
    14161 cagacccgcc gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca gctggttgcg
    14221 cgcctcctgc ggcagctgcg caacagcttc aaagtagtag caaatctgcg ccagcaaacg
    14281 gctgatgtta atcgagttag ccgagtttaa ccccagcgcc actttcagtt cttcatcatc
    14341 aaacgcctgc ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc
    14401 gatattgccg cccaacgtac agaacagttt ttcttgcagt ggactgattt tgcctcgtgg
    14461 atagaggata accactttca cattcggcaa accgtagaaa gcatgagcca ctgcagctcc
    14521 ggtatcaccg gaggtcgcgg tcagaatggt cactggctta tcatccgcaa tatgggtcag
    14581 catttgtgcc ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa
    14641 ttccagacaa ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg caaacgccgc
    14701 gcgcacgcgc tcttccagga tttcctgtgg gatttcatca ccaataaacg ccgagaggat
    14761 cttcgcactg cgggtgacaa aatccagctt cagcatctca tcaatttcag tcaggctgaa
    14821 ttccggcagg tcgtgcggaa aaaacagccc ctgatttttg cccaacccct gggttacggc
    14881 ttgcgcaaag ctgacctgct cgttgtgatc tttcagattg tagagtttca tttagttttc
    14941 cagtactcgt gcgcccgccg tatccagccg gcaaatatga acaaaacctt cctgattttg
    15001 caggtagttc ttacccaacc agtcggcaac gcgctgggcg gtatccggct tgtcacatag
    15061 agcgaacaag gtcgggccgg agccggagat accgctcgct accgcgccga tttccgcgac
    15121 cgcctgccgc gcctgccgga agccaggcag taaccgttca cggtagggtt cagcgataac
    15181 atctttcatc agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt gaatgaagcc
    15241 agccagatgt cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg gtaaaatagc
    15301 ccgggcttct gccgtcgaga ctttaattcc cggatacgcc agcacccaca gccactcatc
    15361 aaaccctggc acttgctggc tgatgatgtc gttttcttcg atcatcaact gcataccacc
    15421 aagaaaacac ggtgccacgt tgtcgtaatg aatgctgccg gagatacgtc cttccagctc
    15481 gcccatcaaa gccagcaaac gagtgtcatt aagcggcttg ccgcagtgtt cattcatcgc
    15541 catcagcgcc gcgaccaccg aacaggcgct ggagcctaag cccgaaccga tcggcatatt
    15601 cttttccaga gtcatcgcca ctggaatttg cttgccaagc tcctggcaaa aacgctccca
    15661 gcactgataa acgatatttt cccgtggttc tgacggcagc ttatcggcaa agcgtccgag
    15721 tttgttgaga ctgaatgtct ctgccgcctc aaccgtgact acatctccga gcaatgcacc
    15781 atcaacgggt gccaccgccg ccccgagcac atcaaacccg acgctcatat tggcactgga
    15841 agccggggca taaactttaa ccatgtcaga ctcctaactt ccatgagagg gtacgtagca
    15901 gatcggcaaa gacaccggca gcggtaacgt cattgcccgc accatatccg cgcagcacca
    15961 acggcagcgg ctgataatag tggctataaa aggccagggc gttttcgcca tttttcactt
    16021 tgaacagcgg atcattccca tccacttcgg caatcttcac gcggcagaca ccatcttcat
    16081 caatattgcc aacatagcgc aaaacttttc cttcatcacg ggccttcgcc acgcgcgcgg
    16141 caaagagatc gtcgagctgt gacagattcg ccataaaagc ggcaacatca ccctcggcgt
    16201 taaactctgc gggcagcaca ggttcaattt caatatccgc cagctccagt tcacgtcccg
    16261 tttcacgagc gagaatcaat agtttacgcg ccacatccat accagaaaga tcatctcgcg
    16321 ggtccggttc ggtataaccc atttcccgcg ccagcgtggt cgcctcggag aaactcatgc
    16381 cttcgtctaa cttgccgaag atataagaaa gcgaacctga aagaatgccg gagaacttca
    16441 ccaattcatc acctgcattg agcagatttt gcaggttctc aataaccggt aatccagccc
    16501 caacgttggt gtcatagagg aatttacgcc gcgatttttc cgccgcataa cgcaactgat
    16561 gatagtaatc catcgacgag gtgttggcct ttttgttcgg cgtgacaacg tggaaacctt
    16621 cgcgcaggaa gtcggcatat tgatccgcca ctgcctggct ggaggtgcag tcaacaatga
    16681 ccgggttcag cagatgatat tctttcacga ggcgaattaa gcgcccgaga ttaaacggct
    16741 ctttggcttg tgcaagttct tcctgccagt tttccagatt aaggccatgc acactagtga
    16801 gcagagcctt cgagttggca acgccgcaga cacgtaagtc gatatgttta ttcttcagcc
    16861 agctttgctg acgcttcagt tgctccagca gcgcaccgcc aacgccaccg acgccaatca
    16921 caaacacttc gataacctga tcggtattga acagcatctg atgagtaacg cgcacgccag
    16981 tggtcgcatc atcgttattt accacgacag agattgagcg ttcagaagat ccctgagcaa
    17041 tggcgacaat gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac
    17101 gcaaggtgcg cataccatca cctaccaccg agataatggc cagccgttcc gtcaccgcca
    17161 gcggctccag taagccttct ttcagttcca ggtagaactc ttcctgcatt gcccgttcag
    17221 ctcgcacaca gtcgctttgt ggaacgcaga aactgatact gtattcggaa gatgattgcg
    17281 taatcagcac cacggaaata cgggcgcgtg acattgcagc aaagacgcgc gccgccatgc
    17341 caaccatccc tttcatcccc gggccggaaa cgctgaacat tgccatgtta ttcagattgg
    17401 aaatgccctt gaccggtaat tcgtcttcat cacggctggc accaatgagc gtacctggtg
    17461 cttgaggatt tccggtattt ttaatcaggc aagggatctg gaactgggcg atgggggtaa
    17521 tggtgcgggg gtgaagaact ttagcgccga agtaggaaag ctccatcgct tcctggtagg
    17581 acatcgactt caacaacctc gcatcgggca cctgacgcgg gtcgcaggta tagaccccgt
    17641 caacgtccgt ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg
    17701 agtagtcgga accgttacgt ccaagcacca ccagttcgcc tttttcatta ccggcggtga
    17761 aacctgccat caacaccatg tgatcagccg gaatgcggct tgccgcaata cggcgggtgg
    17821 actcagcaat atcgacggta gattcgaggt aatgccccac tgccagcagt ttttcgaccg
    17881 gatcgataac ggtaacgttg tgaccacgcg cttctaacac gccggccata atggcgatcg
    17941 acattttctc gccacggcaa atcagcgcag cgttgatgct atccgggcac tgccccaaca
    18001 aactaatgcc atgcaggaca tgttttattt gggcaaattc ctgatcgacg aaagttttca
    18061 attgcgccag cgggaacccc ggctgggcgg cggcgagtcc cgtcaaaagt tcggcaaaaa
    18121 tacgttcggc atcgctgata ttgggtaaag catcctggcc gctaatggtt ttttcaatca
    18181 tcgccaccag gtggttggtg attttggcgg gggcagagag gacggtggcc acctgcccct
    18241 gcctggcatt gctttccaga atatcggcaa cacgcagaaa acgttctgca tttgccactg
    18301 atgtaccgcc gaacttcaac actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa
    18361 aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc
    18421 gttacctgtg gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt
    18481 tgtgtactct gtaattttta tctgtttgtg cgctatgcct atattggtta aagtatttag
    18541 tgacctaagt caataaaatt ttaatttact cacggcaggt aaccagttca gaagctgcta
    18601 tcagacactc tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa
    18661 gctgaaaaat acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag
    18721 caaacggtgc aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gctgtaacca
    18781 gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tgactcgttc
    18841 gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca cttttcgccg gttgttgtat
    18901 taatgttgct aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata
    18961 atccgccacc atcggcacac cagtaagcac gtcagccaac gctaactctt cgttagtcag
    19021 cccggaatct tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc
    19081 taacagcggc accagttcta ctggcgtggc gtagtaatga tatttcgccc gactacgcgc
    19141 agtggtggcg acagtgaaat cgacatcgtg taacgattca gccagtgtcg ggaaaacttt
    19201 aatattatca ataatatcac cagatccatg tgcgacccag cgggtggctg gctccaggtg
    19261 tgcctgacta tcgacaatcc gcagttcgct aaaacccatc gttttcattg cccgcgccgc
    19321 tgccccaata ttttctgctc tggcgggtgc gaccaggata atcgttatac gcatattgcc
    19381 actcttcttg accaaataac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg
    19441 aatttacaaa ttcttaacgt aagccgcaga aaaagccctt tacttagctt aaaaaaggct
    19501 aaactatttt ctgactgcac taacggttta attgttaaaa aatgctacat atccttctgt
    19561 ttacttagga taattttata aaaaataaat ctcgacaatt ggattcacca cgtttattag
    19621 ttgtatgatg caactagttg gattattaaa ataatgtgac gaaagctagc atttagatac
    19681 gatgatttca tcaaactgtt aacgtgctac aattgaactt gatatatgtc aacgaagcgt
    19741 agttttattg ggtgtccggc ccctcttagc ctgttatgtt gctgttaaaa tggttaggat
    19801 gacagccgtt tttgacactg tcgggtcctg agggaaagta cccacgacca agctaatgat
    19861 gttgttgacg ttgatggaaa gtgcatcaag aacgcaatta cgtactttag tcatgttacg
    19921 ccgatcatgt taatttgcag catgcatcag gcaggtcagg gacttttgta cttcctgttt
    19981 cgatttagtt ggcaatttag gtagcaaaca tgcagacccc gcacattctt atcgttgaag
    20041 acgagttggt aacacgcaac acgttgaaaa gtattttcga agcggaaggc tatgatgttt
    20101 tcgaagcgac agatggcgcg gaaatgcatc agatcctctc tgaatatgac atcaacctgg
    20161 tgatcatgga tatcaatctg ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg
    20221 agcaggcgaa tgttgcgttg atgttcctga ctggccgtga caacgaagtc gataaaattc
    20281 tcggcctcga aatcggtgca gatgactaca tcaccaaacc gttcaacccg cgtgaactga
    20341 cgattcgtgc acgcaacctg ctgtcccgta ccatgaatct gggtactgtc agcgaagaac
    20401 gtcgtagcgt tgaaagctac aagttcaatg gttgggaact ggacatcaac agccgttcgt
    20461 tgatcggccc tgatggcgag cagtacaagc tgccgcgcag cgagttccgc gccatgcttc
    20521 acttctgtga aaacccaggc aaaattcagt ctcgtgctga actgctgaag aaaatgaccg
    20581 gccgtgagct gaaaccgcac gaccgtactg tagacgtgac gatccgccgt attcgtaaac
    20641 atttcgaatc tacgccggat acgccggaaa tcatcgccac cattcacggt gaaggttatc
    20701 gcttctgcgg tgatctggaa gattaatcgg ctttaccacc gtcaaaaaaa cggcgctttt
    20761 tagcgccgtt tttatttttc aaccttattt ccagatacgt aattcatcgt ccgttgtaac
    20821 ttctttactg gctttcattt tcggcagtga aaacgcatac cagtctatat tacgagtcac
    20881 aaacatcatg ccggccagcg ccaccaccag cacactggtt cccaacaaca gcgcgctatc
    20941 ggcagagttg agcagtcccc acatcactcc atccagcaac aacagcgcga gggtaaacaa
    21001 catgctattg cgccagcctt tcaataccgc ctgcaaataa ataccgttca ttaacgcccc
    21061 aatcagactg gcgattatcc atgccacggt aaaaccgata tgttcagaaa gcgccagcaa
    21121 gagcaaataa aacatcacca atgaaagccc caccagcaaa tattgcattg ggtgtaaacg
    21181 ttgcgcggtg agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat
    21241 ggcgtactta gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg tcactgcgac
    21301 gctaaacgcg gggaagtttt cccagccggt atcattgcct gaagcaaaac gctcaccgag
    21361 attattagca aaccagctgc tttgccattg cgcctgaaaa cctgactcgc tagcttcccg
    21421 tttggctggt agaaaatcac ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat
    21481 ttcgctatta cgcccggcag gcaccacaga aagatcgccg gtaccgctta aattcagtgc
    21541 catattcagc tgcaggttct gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc
    21601 ctgcccgcct tgctctaacc cggtaccggg ttcaatggtc agtgccgttc cgttaacttc
    21661 aggcgctttc accacaccaa taccacgcgc atccccgacg ctaatcacaa taaagggctt
    21721 gcctaagatg atatttggcg cgttgagctc gctaagacgc gaaacatcga aatcggcttt
    21781 tagcgttaaa tcactgtgcc agacctgacc ggtataaatc cctatcttgc gttcttccac
    21841 gttctgattg ccatcaacca tcaatgactc aggtaaccaa aaatggataa aacttcgttt
    21901 ccgctccacg gttttatcct cttcctgcac cgtataaagc tcggtcacag gaatagcgat
    21961 aagcggccca acgagttttt gcggcccgct ggtactttgg cgaatcgcat cttccacatc
    22021 gctacggtaa tcggcacgtt cgacaatcac ttgccgaatc agcattatcg gaatcaacaa
    22081 cagcaatact gcaccaaaca aggtggtcat tttccagaac aggggggatt tcaacatagt
    22141 cttctccttt gcaatggcag caggatacga aggctatgtg ggaagaattt gaagctatgt
    22201 gaagtgacgg tgaagtcgta gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt
    22261 gacttcgccg ttaaacaaac gggcgacctc actgacgaac gccaacccca gaccgctgct
    22321 tttttgccca tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg acagcgcgta
    22381 gtcaggaata ccactaccgg tatccagcac cttaagcgtg acgtgttcct gatccacttc
    22441 ggcgcttagc gttatgcaac cgctctcggg agtaaaatcg atggcgttat ccagtaaatt
    22501 ccccagcgcc tgatccagta acgccggttc agcagcaaca ttaacctcgg tgggcataac
    22561 atgcaaagtg atgttttttt ctgccaactg cacggtgcgc gcttcgctga cgcggcgaaa
    22621 taatgccgcc acatcaacag cagtcagaac gacttcctga cgattctcca gtcttgcctg
    22681 gcgtagtaac gtttccacca gcgcctgcat tcgcgcattt tgtgtcagaa tgttgtcagt
    22741 aaaacgagcc actacttcag gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat
    22801 cgccgccagt gggcttttta gctcatgggt taacgcataa acatactgct caatatagtt
    22861 tttcccttcc agcttcacgc gcatactttc cagcgcctgc gcgagtttac gcagctcgct
    22921 actaccgaga tcgggaagag gaacgggctt attgtcagtg acggaatcag catagcgagt
    22981 gagccgggca atggagcggt tgatccacca aaccatgcct gcgccaatca ccagtgcaat
    23041 ccccaacaaa atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc
    23101 catcgccgcg ttcggtttgc ctacgctcaa aacgccaata agccgcgagc cgcccataat
    23161 cggcgcggca acatacatca ccgaactttc gggatcggca ggattttgca acgtgctgcg
    23221 cgcaccatac tgaccacgca acgttagcca gacgtcattc cagcgcgaat aatcctgtcc
    23281 aacggcttta tttgccgaat cgaacaatac tttgccatgc gcatcggtca tatagacatg
    23341 atattcattg cgcactttgt taatgccacc aatattggcg cgaaacgggc gatgttgtag
    23401 ctgattaaac gcctgcgcca gttgcccatg cgttgggtcc ccagagagca aatcgggacg
    23461 cgccagctcc gcaagtaacg ttgccgtgtc gatcaacgtc ccctccgttg ctcttcgcac
    23521 gcccggttta acttctttga caaaaatagc cagtacgaac caggccgcca ccgccaccag
    23581 taaaaaatag cccagcagca accgcatgcc gatacgcatt acaagcccct caggctatat
    23641 cccatgccgc gatgagtatt aatcggtgaa agatcggggt tgatggcgcg cagcttggca
    23701 cgcagcgttt taatgtggat atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac
    23761 acgctatcca ttagttgctg gcgggaccag acgcggcctg gcgacttgag taacgtcttc
    23821 agcaataaaa actcataccg cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc
    23881 gcgggttcat tcaattcaaa atgaccaata cggatgacgg gagacggcgt cgagaacttc
    23941 ttcacccgac gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga aaacggttta
    24001 gccacatagt cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc ttcacttcgg
    24061 gccgtcagga acagtacagg taacgccgga tagagcgcca gtaactggcg gcacaattca
    24121 aagccactga tatcaggcaa accaacgtcg agaatcatga cgtcgggaac ctgctggcga
    24181 gctttatcca gcaccggcaa acctcgctca aagacctcga cggcaaaacc ttcctgctgc
    24241 aacatgtaga ccagcgtgtc ggctatccct tgctcatctt ccactaacca gaccgtttcc
    24301 cgttgcataa atcgcctctg ttattgccgc cacggcatga caggaaccgc gctaatcgcg
    24361 tttttcggcg aaccttctac aactttgtcg gagtaagcca gataagcgag cgcgttgcgt
    24421 ttggcgtcat aaaagcgcac gacctgtaac gacttaaaga ccagggacgt gcgttttttg
    24481 aatactacct cgccctgagc tttgccgttt ttaatacgat ccgacagttc aatcggcccg
    24541 acttgctgac aagaaatggc cgcatcggag gtatcttctg ccaggcccaa tcccccttta
    24601 ataccaccgg ttttcgcccg gctcacataa caggtgacat ttttcacatc gggatcgtca
    24661 aaggcttcca caacaatttt gtgatccggg ccgatcattt taaatacggt gtcgaccgaa
    24721 ccaatctctt ctgcatgagc caatggcccc agcattatca ggcttaaaga caggatcaaa
    24781 tgcttgtatt tcatattgtt accattttta tctattacac gagcaattag ttcgtcacgg
    24841 taaataacat attgattaag aacaaaatct caattctttt atttccgtca agccctaaaa
    24901 catactctac taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct attatccaat
    24961 tacctgatgt caggtgctcg ttgttgaaag gatgaggata ttttatggat caggccggca
    25021 ttattcgcga ccttttaatc tggctggaag gtcatctgga tcagcccctg tcgctcgaca
    25081 atgtagcggc gaaagcaggt tattccaagt ggcacttaca gagaatgttt aaagatgtca
    25141 ctggccatgc tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac
    25201 tacgcctgac tgcgcgtccg attctggaca tcgcgctgca ataccgcttc gactctcaac
    25261 agacatttac ccgcgcattc aagaagcagt ttgcccagac tcctgcactt taccgccgtt
    25321 ctcctgaatg gagcgccttt ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc
    25381 cagagcacaa atttgtcacc ctggaagata cgccgctgat tggtgttacc cagagctact
    25441 cctgttcgct ggagcaaatc tctgatttcc gccatgaaat gcgttatcag ttctggcacg
    25501 attttctcgg caacgcgccg accattccgc cggtgctcta cggcctgaat gaaacgcgtc
    25561 cgagtcagga taaagacgac gaacaagagg tattctatac caccgcgtta gcccaggatc
    25621 aggcagatgg ctatgtactg acggggcatc cggtgatgct gcagggcggc gaatatgtga
    25681 tgtttaccta tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa
    25741 cgtgcatgcc aatgctcaat ctgacgcgcc gtaaaggtca ggatattgag cgatactacc
    25801 cggcagaaga cgccaaagcg ggagatcgcc caattaatct gcgctgtgaa ctgctgattc
    25861 ctatccgccg ttaacgctgc agctcatcta atgcaggggc gtccagatgc gagatgtccc
    25921 ctgcggtttc aacgacccag ccagacgcca gccacaggct ttcctgataa tccacgcgcg
    25981 aaatagaaca gttacgcaga cgtaagcggc gctctgccca tgctggtaat ccgagaatcg
    26041 tactcaccag acatcccagt gcaataccat ggctcaccag cagcggtcgg cttccctgcg
    26101 gtaagtcccg gcaagattcc agtgccgcat tgacgcgatc gctgagctcc tgcattgact
    26161 cgccttcagg aatacgcccg tcaacggtgc cattgaccag ctgccgacgc cagttctctt
    26221 cttcttcggt cagagaatcg atatgtcttt tttccagcac acccatgttt aattcacgca
    26281 ggcgagaatc aaagatgatg tcacagccgc aggcctgggc gatgatttcc gccgtacgcc
    26341 gggtgcgtcc taaatcgcta ctgataatat gcgtaatgcc aagctctttg gcacgggttg
    26401 ccacctgcat cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac tggccctgaa
    26461 tacgtcgctc ggcgttccac tgcgtttcac cgtggcggac taggtatacc tgtaacatgc
    26521 tttttttccg tatactgctg taacgttaat tttctgagta atgctgatta tgcaccaagt
    26581 tgtctgtgcg accaccaatc ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat
    26641 cttcggcgaa ggatcctgcc atattgcatc cgttgccgtc gagagcggtg taccggaaca
    26701 accctttggc agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg
    26761 tttacttcca gaggctgatt tttgggtggc gattgaagct ggcattgatg gcgacagcac
    26821 tttcagttgg gtggtgattg aaaacgccag ccagcgcggc gaggcgcgtt cggcaacttt
    26881 gccgttacct gcggtgattc tggaaaaagt tcgtgaaggc gaggcgcttg gtcccgtgag
    26941 tcgcgttata ccggtattga tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc
    27001 gccgggaaac tcactcgcgc cagcgtctat caccaggcgg tgatcctggc gctgagtccg
    27061 tttcacaatg ccgtgtatca ggcattgtag gacggataag gcgttcacgc cgcatccgac
    27121 attcggtgca cgatgcctga tgcgacgctg ccgcgtctta tcaggcctac aaaatcaatc
    27181 gcttttcagc aacacctctt ccagccactg acgcagctcc acgggcgcgg ctttcaggct
    27241 gttagatcca cgcgtaatcg tcgcgatgcc cgcgccgagt tcatttttta actcacgctg
    27301 actcatttcg ccacgcaaca gctcttcgac aatacgcacg cgagtcccca acgcttcgcg
    27361 ctcatctggc gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt
    27421 cttaagcagg tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca tcgctgctga
    27481 atagggtgat tgttgggcca taatatgtcg ccattgttag cgggggaagc aaaatgactc
    27541 ccccggttgt actcgctaaa gagtacgata gcatatcata aacgtgcgga tcagtaacga
    27601 cgtccccatt ccgtggcgct catcaacgtc ggtttatccc ccatgaaata gcggtagtaa
    27661 gcatcataag ccagcacgtt cttcacataa ccgcgcgtct cggagaacgg aatactctcg
    27721 acaaatgcca cagcgtcgat acgcccggcg ctgttgccaa gccaggttcg cacccgtcca
    27781 ggtccggcgt tataagctgc tgaggagaaa atacggttat tgccaaactg ctgataaaca
    27841 tattgcaggt aactggtgcc aatgttgatg ttcgtttccg gatccagcaa ttgcccaggg
    27901 ctgctataac caggaataga gaacatcttc accgtatggg tcgctgtacc aggcataatc
    27961 tgcatcaggc cgctggcccc taccggtgat ttcactttcg gattccaggc gctctcctga
    28021 cgggcaatcg ccatcgcata gctttgcggg atctctttcc cactggtgta acgtttgaaa
    28081 agatcgttgt aagccagcgg gaatcgctct tccagatgat cccaaagctt cccggcgatc
    28141 gttgcctgaa cgctaagatc ccaccattgg ttattgaaag cataccgagc cagttgagcc
    28201 tgctctgttt ttgacttgct cttcaccaga ttggcccact cgctacgcgc ggtattatcg
    28261 agattccagt acatcaactc gcgcacgcgc gccatctccg gcccctgagt cagggtgctg
    28321 tcaacattct gcggcgcttt atcaatcttc agctcatact cttcaccgat gcgttgtgct
    28381 gcaaccatcg ggtagaaacc acgctgttgt atgagttgat gcaaaatctc tttcgcttca
    28441 gcttcacgtc cgcgttccag caataaatcc gcctgccagt aacgccattc atctttctct
    28501 tttgcttcca tcggcagacg cgccagccag gtattcaggc cgcgacgatc gccggtgcca
    28561 agcgccattc gcacacggcg ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca
    28621 tcgcgccatt tcgcctgctc atcggtgaca tcattgccca tcaaacgcca ggcgacgata
    28681 tcgcgcagct cctgaatctg atcttcatta agctgctgcg cctgggcaag cgacgggatc
    28741 atcagccgtg cattttcggc atcctgccgc gccacactgg caaacgccac cgccgccatt
    28801 tgacgggtaa aatcggtcgc gccggttgta cgcgcgaagg tcagtaccgt attagggttg
    28861 ttcgccagtg aaatgattgc cgaggcgata gtctggtaat cggcaggcat ctgccctgcc
    28921 agcactgtta ccaggcctgt gttacccgct ttcatcgcca gacggatacg ctctaaatac
    28981 gccagcggat cttgtttacc tgacgcacgc cagacgctaa ataacttgtc acaggcgtta
    29041 ggctggctct tgccggttag ccacagctct ttcgcccctt gccaggcttc ttcactctgc
    29101 ccggtgttcc atttcgcata gtagtaatta cattgcgctt cggtagttcc gggcttttcc
    29161 gggctaaagg ctaacaagcc acgccagtct tcacgccgcg ccagttcatt gacgaaacga
    29221 gattgcagcg tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc
    29281 accgccggtt gattcatcag gtcatcggtg atctggcggt attccaggta gggataaagc
    29341 ggataatcct tcagtccagg catcatttgt tcgaccacat ccatctgtcg attatcccag
    29401 gcctgcttga tttgcgcgta acggctacgc tgctcatcca gtgagtcggc tcgcgccacg
    29461 ctgctgaccg tcagcagaca gacaccggca gccaacagcc gccaggtaac ttgtttggct
    29521 ttttccacaa gcgcatcctc taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata
    29581 gatttatgct aaccagtcat tgccgtttac gccacgttac ggacactttt ttacttttac
    29641 tgcgaaggtg atcgaccaac tgggattagg cggggaaaca ggctacactt cgcctttgaa
    29701 aactcatcat cacgataaac atagaggcga agtccaacgt ggctcaattc gtttatacca
    29761 tgcatcgtgt cggcaaagtt gttccgccga aacgtcatat tttgaaaaac atctctctga
    29821 gtttcttccc tggggcaaaa attggtgtcc tgggtctgaa cggcgcgggt aagtccaccc
    29881 tgctgcgcat tatggcgggc attgataaag acatcgaagg tgaagcgcgt ccgcagccag
    29941 acatcaagat tggttatctg ccgcaggaac cgcagctgaa cccggaacac accgtgcgtg
    30001 agtccattga agaagcggtt tcagaagtgg ttaacgccct gaaacgcctg gatgaagtgt
    30061 atgcgctgta cgccgatccg gatgccgatt ttgacaagct ggccgctgaa caaggccgtc
    30121 tggaagagat cattcaggct cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg
    30181 cggatgcgct gcgtctgcca gattgggacg cgaaaatcgc taacctctcc ggtggtgagc
    30241 gtcgtcgcgt ggcgttgtgc cgcctgctgc tggaaaaacc agacatgctg ctgctcgacg
    30301 aaccgaccaa ccacctggat gccgaatccg tggcgtggct ggaacgcttc ctgcacgact
    30361 tcgaaggcac cgtggtggcg attactcacg accgttactt cctcgataac gttgcaggct
    30421 ggatcctcga actggaccgc ggtgaaggaa ttccgtggga aggtaactac tcctcctggc
    30481 tggagcagaa agatcagcgt ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt
    30541 cgatcgagaa agagctggag tgggtacgtc agggaactaa aggccgccag tcgaaaggta
    30601 aagcacgtct ggcacgcttt gaagagctga acagcaccga ataccagaaa cgtaacgaaa
    30661 ccaacgaact gtttattcca cctggaccgc gtctgggcga taaagtgctg gaagtcagca
    30721 acctgcgtaa atcctacggc gatcgcctgc tgattgatga cctgagcttc tcgatcccga
    30781 aaggggcgat cgtcgggatc atcggtccga acggcgcggg taaatcgacc ctgttccgta
    30841 tgatctccgg tcaggaacag ccggacagcg gcaccatcac tttgggtgaa acggtgaaac
    30901 tggcatcggt tgatcagttc cgtgactcaa tggataacag caaaaccgtt tgggaagaag
    30961 tttccggcgg gctggatatt atgaagatcg gcaacaccga gatgccaagc cgcgcctacg
    31021 ttggccgctt taactttaaa ggggttgatc agggtaaacg tgttggcgaa ctttccggtg
    31081 gtgagcgcgg tcgtctgcat ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc
    31141 tcgacgaacc aaccaacgac ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc
    31201 tggagttccc gggctgtgcg atggttatct cgcacgaccg ttggttcctc gaccgtatcg
    31261 ccacgcacat cctggactac caggatgaag gtaaagttga gttcttcgaa ggtaacttta
    31321 ctgagtacga agagtacaaa aaacgcacgc tgggcgcaga cgcgctggag ccgaagcgta
    31381 tcaagtacaa gcgtattgcg aagtaatgcg taaaatgccg gatgcggcgc gaacgcctta
    31441 tccggcctac aaaaccagca atttcaatac gttgtaaaac tgtaggcctg ataagacgcg
    31501 gtaagcgtcg catcaggcat tgagcaccga atgccggatg cggcgtgaac accttttccg
    31561 gtctacaaca gccttagcat cctcaatttc gtggcgggtc acccgtgcag gttttttcaa
    31621 accttgttta atctcgacag cctcttccag agaggttttc aactcatcaa aaaaactcat
    31681 tttatacctc gcctttgagc agtttcgtca cggttatcct tgctccccca tcatctcccg
    31741 caccagctcc acgcagcgca ggaaacggct gtcgtaatcg tcctcttcaa cccgcacgaa
    31801 ttcgatattg ttctcttcca gcatctccac cagcaagttc tggaactctt tgcgatccac
    31861 cgaactgccg aggctgcgta aaccatccgc cacccacggc gtgttgttct ccagcaggat
    31921 caccagatcg aaacggtatt catcaatcag cgcctgcacg aacggatgct cacgcccttc
    31981 gtactttttg cagaacgcct gggtagtgac aaaatcggtg tcaataaacg ccactttatt
    32041 ggcgtatttc actgcaaaat caatgtattg tgcgtgaccc aacgcgattt tatcgtagtc
    32101 ggaatactgc aatgcgattt catcaccgcc gaggtgtgaa aagacataat cgcggccata
    32161 ttcccacgca ctggtggtgt tgaagatatt ggcaagtttg tttaccaggg tggatttacc
    32221 gctcgactcg ccgccaagga tcgccacggt acgcacaaaa aacggcttca cttcggtagg
    32281 aatatattcc cagtagcgga acgggttttc gcggatctgc gcaccgctga tactcataaa
    32341 ggtacgtttc ggatcgacca gcaccgtctc gatccccaga tgttccatat actgtggcgc
    32401 atcggcttct tccgaggtgt agatcaggtc cggctggatc cccttttcag ccataaactt
    32461 tttgatgccg ttgctccaca catcccagcc atgcggatac ggctccatgc cctcttcgtt
    32521 gaaagcatga atacggatat ttttctgata tttaaaggtt tgcaatagcc agcgcagacg
    32581 atccggcacc gtgggctgct gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt
    32641 atcgtcaaaa cccataatga tatgcagctc gtcaacctgg ctacaggcgc gctggataag
    32701 gtagatatgt ccggtatgca gcgggtagaa cttaccgaat acgacaccaa tggttttctt
    32761 ctgccgggga aattcaagcc ccaaaaaacg gtgcaatgcc tccagctttt gcgcgctggg
    32821 gcttttaatt ttggcattca gtaactggct taaataccct ttggtcatac cgctggcatc
    32881 agctacctgc tgtagcgtgc agccctgttg cttgatggca gttttcaggt aatcaaatga
    32941 cgacatatct ccctccgtat ctctcattat aagtcgtcga acacgctaag cgcgtcggag
    33001 agttttttaa cgccaaaaat ctgcatccct tccggcgctt ttttcggtac gttagccgcc
    33061 ggaacaatcg cccggcgaaa accgtgtttc gccgcttcag aaatccgctc ctggccactg
    33121 ggcaccgggc ggatctcccc tgccagcccg acttcaccaa acaccaccag atcctgtggc
    33181 agcggtctgt cacgcaggct ggaaaccatc gccagcagta acgctaagtc ggcgctggtt
    33241 tcggttacct tcacgccgcc gaccacgttc acaaacacat cctgatcggc catttgcaga
    33301 ccaccgtgac ggtgcaagac agccagcagg attgccagac ggttttgttc cagccccact
    33361 gccacgcggc gcggattcgc cattatcgag tgatcgacca gcgcctgaat ctccaccagc
    33421 aacggacgcg ttccttccca caccaccatc acggagctgc cggaggtcac ttcatctccg
    33481 cgacttaaga aaattgccga agggttgctg acttcacgca gcccctgttc ggtcatcgcg
    33541 aagacgccca gctcattcac cgcgccgaag cggtttttat ggctgcgtaa ggtgcgaaaa
    33601 cgggagtcgg catcgccatc cagaagcacc gaacagtcga tacagtgttc cagcactttc
    33661 gggccagcca gcgaaccatc tttggttacg tgtccaacca tgacaatcgc cacaccgcgc
    33721 gttttggcga aacgcgtcag ataagccgcc gtttcacgca cctgcgccac gctaccaggc
    33781 gatgattgta catccgccat atgcatcacc tggatcgagt caattaccat cagcttcggt
    33841 tgctcttctt cggcaatcag gcagatctgc tcgatgctgg tttccgacaa catattgaga
    33901 ttgtcagtcg gcaggccaag gcgatgagcg cgcattgcca cctgttgcag cgactcttcg
    33961 ccggtgacat acagcgtttt catctgctgg gccagtttgc acagcgtttg cagcaacagc
    34021 gtggatttcc ccgcaccagg gttaccgcca atcagaatgg cacttcctgg caccacgccg
    34081 cctcctagta cgcggtcgaa ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg
    34141 ctgatatcgg agagtttctg gacttttgcc accccggcgc taccggcata gccgctgaga
    34201 cgctcgttac gcgccaccgt tggcgacgca gcaagacgca cctcggtgat ggtgttccag
    34261 gcatgacagg cactgcactg cccctgccag cgcggataat cggccccgca ttcattacaa
    34321 acaaaggcgc gttttggagc ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca
    34381 ggatgacggg caggcaatta cttctgattc aggctgccag agaggatgca gaataccccc
    34441 atcaggtcag cgtgacggat ggtgacttcc gccttttcat tcacttttgg cttggcatgg
    34501 taggcaatcc ccagccctgc tgctttgatc atcggcaggt cattagctcc atcgccaatc
    34561 gccacggtct gcgccagcgg gatttcatac tcctgcgcga ggcgagtcag agttttcgct
    34621 ttgtactgcg cgtctacgat gtcgccgatc acattgccgg taaatttacc gtccatgatc
    34681 tccagttcat tggctaccac ggctgtcagg cgcagcttgt cgcgcaggta ttcagcaaag
    34741 aaagtaaagc cgccggaggc aatcgccact ttccagccca gcgtttccag cttgagcacc
    34801 agctgcgtta agcctggcat cagcggcaga ttttcacgca cctgttgcag aatattggcg
    34861 tcagcgcctt tcagcgtcgc cacgcggctg cgcaggctgg cggtaaaatc gagttcgccg
    34921 cgcatcgccc gttcggttac ctccgccacc atctcgcccg ttccggccag cttggcaatt
    34981 tcatcaatac attcaatctg gatggcggtg gaatccatgt ccatcaccag caaacccggc
    35041 gtgcgcaggt gcgggatttt ccccagcggg gcgacatcca gctgcgcttc gtgggccagg
    35101 cgtgtagccc gtgcggtgag tgaacctgcc agacgaatca cctgataatc ttccacgcac
    35161 caggcggcaa caatcaccat cgccgcaccc agtttgctct ggtattgggt cagacgctgt
    35221 ttatccagcc cacgaccata cagcagccag ccgctacgac ctgcgtggta atccagtggc
    35281 atcacttcat caccacttaa tgaaagaggc agacccggcc ataaagagac atcttcaggc
    35341 aggtcgcacc aggtaatgtt aggcattaag gctcctgtaa aatcgttcga agcagggaaa
    35401 ataacgcatg aggctacctt gtatccattg cttctggcaa cattaagtct caaattttca
    35461 aagggtggaa gatggctcgc acaaaactga aattccggct acatcgggca gtgattgtcc
    35521 tgttctgtct tgccttgtta gtggcactga tgcagggggc gtcatggttt agtcaaaacc
    35581 accagcgaca gcgtaatcca cagctggaag aactggcccg caccctggcg cgtcaggtga
    35641 cgctgaacgt tgcaccgctg atgcgtaccg actcaccgga tgaaaaacgc attcaggcga
    35701 tcctcgatca gttaacagat gaaagccgta tcctcgacgc gggtgtgtat gacgaacaag
    35761 gcgatcttat cgcacgttct ggcgaaagcg tcgaagtgcg cgatcggctg gcgctcgacg
    35821 gtaaaaaagc aggcggctat tttaaccagc agattgtcga gccaattgcg ggtaaaaacg
    35881 gaccgctcgg ctatctgcgc ctgacactcg acacccatac gctcgccacc gaagcccaac
    35941 aggtggataa caccactaac attttacgcc tgatgttgct gctctcactg gcaatcggcg
    36001 tagtgctgac ccgcacgctg ctacagggta aacgcacccg ctggcagcaa tcgcccttcc
    36061 tgttaaccgc cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca
    36121 ttactacaag aaaggaaatc gttatgtcca cattacgcct gctcatctct gactcttacg
    36181 acccgtggtt taacctggcg gtggaagagt gtatttttcg ccaaatgccc gccacgcagc
    36241 gcgttctgtt tctctggcgc aatgccgaca cggtagtaat tggtcgcgcg cagaacccgt
    36301 ggaaagagtg taatacccgg cggatggaag aagataacgt ccgcctggcg cggcgcagtg
    36361 gcggtggcgg cgcggtgttc cacgatctcg gcaatacctg ctttaccttt atggctggca
    36421 agccggagta cgataaaact atctccacgt cgattgtgct caatgcgctg aacgcgctcg
    36481 gcgtcagcgc cgaagcgtcc ggacgtaacg atctggtggt gaaaaccgtc gaaggcgacc
    36541 gcaaagtctc aggctcggcc tatcgcgaaa ccaaagatcg cggcttccac cacggcacct
    36601 tgctactcaa tgccgacctc agccgcctgg caaactatct caatccggat aaaaagaaac
    36661 tggcggcgaa aggcattact tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc
    36721 cggggctcac catgagcagg tttgcgaggc cataaccgag gcctttttcg cccattatgg
    36781 cgagcacgtg gaagcggaaa tcatctcccc ggacaaaacg ccagacttgc caaacttcgc
    36841 cgaaaccttt gcccgtcaga gtagctgaga gtggaacttc ggtcaggctc cggcattctc
    36901 gcatctgctg gatgaacgct ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa
    36961 aggccatatc acccgcgccc aggtatttac cgacagcctc aaccccgcgc cgctggaagc
    37021 cctcgccggg cgactgcaag gctgcctgta ccgcgcggat atgctgcaac aagagtgcga
    37081 agcgctgttg gttgacttcc cggaccagga aaaagagcta cgggagttgt cgacgtggat
    37141 agcgggggcg gtaaggtaac tcgctgatta aaatatccaa ggaaggaaat atgatcgatc
    37201 tggaaaacca ggaacgggag attatcaatt taatgttcag ccagcgtatt tcctggctag
    37261 ccgccgtgcg tatcaggcat aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct
    37321 cgattaattc tctaaaacag atcgaaaaga cggaacggct tagctcaaac atcaaaagca
    37381 aaatggcaga aatttatggt tgtccaccag agttgctgat atgcccttcc tggatgacgg
    37441 ctgaacataa atgaatagat tcgtgaatag atacttgaat gttttttatt ttctatccat
    37501 atgaaaaata atagtaaatc tataaatcag ttgctattga tgaatagata ctaaaaagcc
    37561 ggagcagtcc cccactccgg cttaacacga caatcgcctt tcagcgaaac acaattactc
    37621 tttatcgccc agcagaacgg attccagtgc gattttgatc atgtcgttga aggtggtctg
    37681 acgctcagcg gcagtggtct gctcgtgagt gcggatgtgg tcagacacgg tgcagatggt
    37741 cagggctttc gcgccaaact ctgcagccac gccgtagata ccagccgctt ccatttccac
    37801 gccgaggatg ccgtattttt ccatcacgtc gaacatttcg ccgtccggag agtagaacag
    37861 gtcagcggag aacaggttac ccacgcgagc atcaacgccc agtgctttag ctgcatctac
    37921 tgcgttacgc accatgtcaa agtcagcgat agcggcaaag tcatggtctt taaaacggat
    37981 gcggttaact ttggaatcgg tgcaggcccc cataccgata acgacgtcgc gcagttttac
    38041 gtgcggcaga actgcgccac aggaaccaac gcggataatt ttcttcacgc cgaaatcggt
    38101 gatcagttct ttggtgtaga tggagcagga cgggataccc ataccgtgac ccattacgga
    38161 aattttgcgg cctttgtaag taccagtgaa gcccagcata ccgcgaacgt tgttcacttc
    38221 acgggcatct tcaaggaaag tttcagcaat atacttcgca cgcagcgggt cgcctggcat
    38281 caaaactacg tcagcgaaat cgcccatttc tgcattaatg tgtggggtag ccattgtttt
    38341 atcctttttg tgacataaca aaggcggagt caacgctccg cccaaatcca tcagaacatg
    38401 gctttgccat attccatatc agaagtacca aaatattttg ccagagtctg gccgatatcc
    38461 gcgaaggttt cacggtgacc cagtgagccc ggttttactt tcgggccata taccagtacc
    38521 ggaatgtgtt cacgcgtgtg gtcagtaccg gtccaggtcg gatcgcaacc gtggtcagcg
    38581 gtgaggatca ggatgtcgtc atcgcgcagc agagacatca gctccggcag acggcggtcg
    38641 aacagttcca gacccgcagc ataaccggcg acgtcgcggc ggtggcccca ggaagagtcg
    38701 aagtcaacga agttggtgaa gacgatggtg ttatcacccg cttctttcat ctctttgatg
    38761 gtggcgtcaa acagcgcgtc caagccagtc gctttcactt ttttggtgat accgcagttg
    38821 gcgtagatgt ctgcaatttt accgacagaa accacctggc cgtgtttttc atcaaccagt
    38881 ttctgcagca cggtcggtgc tggcggctca acagccaagt cgtgacggtt accggtacgc
    38941 tggaagttac cagctttgtc accgataaac ggacgagcga taacacgacc gatattgtag
    39001 ccgccgttgg tcagctcttc acgggcgatt tcgcacagtt cgtagagttt atccagaccg
    39061 aaagtttctt catggcaggc aatctggaac acggagtcag cggaggtata gaaaatcggc
    39121 ttgccggttt tcatgtgctc ttcgcccagt tgatccagaa tgaccgtacc ggaagagtgg
    39181 cagttaccga ggtaacccgg cagattagcg cgttcgacca gtttatccag cagctcttgc
    39241 gggaagctgt tttcgtgatc ggagaaatat ccccactcaa acagaaccgg gacaccggcg
    39301 atttcccagt gaccagacgg ggtatcttta ccggatgaca tttcgtgcgc ccatgcgtac
    39361 gcgccgataa cttcagcgtt gccgtccatt cccgccggaa tgaaaccggt agaaccttcg
    39421 tgtgctttcg ccagccccag acgggtcaga tttggcagat tgagcggccc tttacgacca
    39481 ttatcagctt cgcctttggc acaagcttct gcgatatgac ccagggtgtc agccccgacg
    39541 tcaccaaagc gttctgcatc ttctgtagcg ccgatgccga atgagtccag caccataata
    39601 aatgcacgtt tcatatgttc tccgtactca gtgcttcaaa tttaaatgat cagatcagta
    39661 taccgttatt cgctgatacg gcgatagaca gttggtgtgc tttccggtgc gttatcggca
    39721 agtttaattg ccgctttcac cgctttcgcc gcttcctgcc agctgttttc gtctttcgcg
    39781 aggataaccg ccagcggacg ctgaccgtct acctggtcgc ccagacgcgc catatcagta
    39841 aagccgacgc tgtaatcgat ggtgtcagat gcctgacgac gaccgccgcc cattgcaact
    39901 actgccatcc ccagcgcgcg ggtatccatt tcactgacaa aaccttcggt atcagcatag
    39961 actgctttcg tcagcatcgc tgtcggcaga tacttcgcgt agttctcaac gaagtcggtc
    40021 gggccttttt gtgccgctac catacgacca aagacttctg ccgctttacc gttgtccagc
    40081 accgcctgca atttcgcgcg cgcttcggcg tcatctttcg ccagtttgcc ggagatcaac
    40141 atctccacgc acagcgccat cgtgacatca aacagacgcg ggttacgata ctcacccgtc
    40201 aggaactgca ccgcttcacg aacttcaacc gcgttacctg cactggaggc cagtacctga
    40261 ttcatgtcgg tgagcagcgc ggtggtgcgc acgccagcgc cgttagccac gccaacaatc
    40321 gcttcggcaa gggcttcaga gagttcgtag gtcggcataa acgcgccgct acccactttc
    40381 acgtccatca ccagcgcgtc cagaccttcc gccagtttct tcgccaggat cgaggcagtg
    40441 atcagcggga tggagtccac ggttgcggta atatcacggg tcgcgtagaa acgtttatca
    40501 gccggagcca gtgaactggt ctgaccgata atcgccacgc cgacgtcttt aataatttcg
    40561 cggaaacggt tgtcatccgg gaaaatgtcg aagccaggga tggattccag tttgtcgagc
    40621 gtaccgccag tatgaccgag gccgcgaccg gagatcatcg gaatatagcc gccgcaggct
    40681 gcgaccatcg gccccaacat cagcgaagtg acatcgccga cgccgccggt cgagtgttta
    40741 tcaacaatcg ggccattcag atgcaggctt ttccagtcga gaacggttcc tgaatctcgc
    40801 atcgccatgg tcagcgagac acgctcaggc attgtcatat cgtggaagaa aatggtcatc
    40861 gcgagggcgg caatctgccc ttcggaaata gtgttgtcgc gaataccgtt gataaagaaa
    40921 cgaatttctt catcgctcag cgcatgacca tcacgttttt tacgaataat ttcttgtgcg
    40981 agaaacaagg taacctcccg aggaaaagag tatgaggcgg cttttgccgg atgcgtctac
    41041 gccttatccg gcctacgggt agatacgtct cgtaggcctg ataagcgcag cgcatcaggc
    41101 gtaaagcatc ttacttagta gctgctggcg ctcttaccgt cgccgtggcc cagcgctttc
    41161 agcaggcttg ccagcaggct ggaagcacca aagcggtagt gacgcgcatc agcccagtca
    41221 gcaccgaaca gttcatctgc gatggcgaga tatttctgcg catcttccgc agtacgcacg
    41281 ccgcccgccg gtttgaaacc aacggttttt tctacgccca tatcacggat cacttccatc
    41341 atgatgcgag cgctttccgg cgtcgcgttc acagccactt taccggtaga ggttttgatg
    41401 aagtccgcac ccgctttgat ggagatttca gacgctttac ggatcagcgc ttcgtctttc
    41461 agttcgccgg tttcgatgat cactttcagc agtacattcg ctgccgcgca agcctcttta
    41521 caggctttca ccaggtcaaa accaacctgc tcgtcacccg ccatcagcgc gcggtacggg
    41581 aacaccacgt caacttcatc agcaccgtag gcgattgccg cacgggtttc tgccagcgcg
    41641 atttcgatgt cgtcgttacc gtgtgggaag ttggttaccg tagcgatacg gatttccggg
    41701 gtgccctgct ctttcagagt tttgcgagca atcgggataa aacgaggata gatacagata
    41761 gcggcggtat tgccgaccgg agttttggcc tgatgacaca gagcaattac tttctcgtcg
    41821 gtgtcgtcgt cattcagggt ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct
    41881 ttcagatcag tcatttcatt ctcctggctt gtcgccaata aaattcacct tgcgagtttg
    41941 ttagtattct aacatctact ccgcaacaca cttcgataca catcacaatt aaggaaatct
    42001 acttacaagt ttgcatcact gtaatgcgat ctggttcaaa taattcactt tcaaatgaat
    42061 gcgtcagtgg tggcaaacgc atcaggatca aagtgaacat cacgaaactt cttacaatgg
    42121 cgcatcacct tcggcataaa aggaaaagaa gatgcccact tctcatgaaa atgcactgca
    42181 acaacgttgc cagcaaattg tcaccagccc agtgcttagc ccggagcaga agcgccattt
    42241 tctggcactg gaagcagaaa acaatctgcc ttacccacag cttcctgccg aagcccgccg
    42301 cgcgctggat gaaggtgtaa tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg
    42361 ctatgtctta cccgattacg ctcgttttct ggcgaacggt tccgaatggc tggagctgga
    42421 aggcgcgaaa gatcttgatg acgcactctc tctgctgacc attctttacc accacgtacc
    42481 gtcggtcaca tcgatgccgg tctacctggg gcaactggat gcgttgttgc aaccgtatgt
    42541 tagaattcta acacaaaacg agatcgatgt tcgaataaaa cgtttctggc gttacctcga
    42601 cagaaccctg ccagatgcct ttatgcacgc caatatcggc ccgtctgatt cgcccattac
    42661 ccgcgcgatc ttacgtgcag acgcgcaggt ttcaccgaac ctgaccttta tctacgatcc
    42721 tgaaatcacc cctgatgacc tgctgctgga agtggcgaag aacatctgtg aatgtagcaa
    42781 accgcacatc gccaacggtc cggtgcatga taaaattttc acaaaagggg gctacgggat
    42841 tgtgagctgt tacaactcac tgccgctggc gggtggtggc agcacgctgg tacgccttaa
    42901 cctgaaagcc attgccgagc gcagcgaatc gctggatgac ttctttacgc gcactctacc
    42961 gcactactgc cagcagcaga tcgccatcat cgatgcgcgg tgtgaattcc tctatcaaca
    43021 atcacacttc tttgagaata gcttcctggt gaaagaaggg ctgattaacc ctgaacgttt
    43081 tgtgccaatg tttggcatgt atgggctggc ggaagcggtt aacttgctgt gtgagaaaga
    43141 agggattgcc gcgcgctacg gtaaagaagc cgccgcaaat gaagtgggtt atcgcatcag
    43201 cgcgcaactg gcggagtttg tcgccaatac ccccgtgaaa tatggctggc aaaaacgcgc
    43261 catgttacac gcacagtcgg ggatcagttc cgatatcggc accacgccgg gcgcgcgttt
    43321 gccgtatggc gatgagccag atccgatcac ccatctgcaa actgtcgccc cgcatcatgc
    43381 ttattattat tccggcatca gcgacattct gacgctcgac gaaaccatca aacgtaaccc
    43441 gcaggcactg gtacagcttt gcctcggtgc ctttaaagcc ggaatgcgtg aatttaccgc
    43501 caatgtcagc ggtaacgatc tggttcgcgt taccggttat atggtgcgtt tgtcggattt
    43561 agaaaaatat cgcgccgaag gttcacgcac caacaccacc tggctgggcg aagaagccgc
    43621 acgcaacact cgtattctgg aacgccagcc gcgcgtgata agccatgaac agcagatgcg
    43681 ctttagtcag taagattatc cccttctcct gcgttgacgg gccaggcagt cgtctggctc
    43741 tgtttttgca gggctgcaat ctgcgctgca aaaactgtca caatccgtgg acgatgggac
    43801 gttgcaatga ctgtggggag tgcgtgccac agtgtccgca tcaggcgttg cagattgttg
    43861 acggcaaagt ggtgtggaac gctgtggtct gcgagcaatg tgatacctgc ctgaagatgt
    43921 gtccgcaaca tgccacacca atggcgcaat ccatgagcgt ggacgaagtg cttagccatg
    43981 tgcgcaaagc ggtgctgttt attgaaggga taacggtaag cggcggcgaa gccacgaccc
    44041 aactgccgtt tgtagtggcg ctgtttactg ctatcaaaaa cgatccgcaa ctgcgccatc
    44101 tcacctgtct ggtggacagt aacggtatgt tgagcgaaac cggctgggaa aaattactcc
    44161 cggtgtgtga cggcgcaatg ctcgatctca aagcatgggg tagcgaatgt catcaacaat
    44221 taaccggacg cgataatcaa cagattaagc gcagcatcta tttgctggca gagcgcggca
    44281 agctggcgga actgcgtttg ctgatgatcc caggccaggt ggattatttg caacacatcg
    44341 aagaactggc ggcgtttatc aagggacttg gtgatgttcc ggttcgcctg aacgcgtttc
    44401 atgcccacgg cgtgtatggc gaggcgcaaa gctgggcgag cgccacgccg gaagacgttg
    44461 agccgttggc tgatgcatta aaggtgcgcg gggtgagccg gttgatattt ccggcgctct
    44521 atttgtgaca tgccggatac ggcctacggt tcggcacaga cttgtaggcc tgataagacg
    44581 cgtcaagcgt cgcatcaggc attgtgcgcc aactgccgga tgcggcgtga acgccttatc
    44641 cggcctacgg ttcggcacaa acctgtaggc atgataagac gcgttaagcg tcgcatcagg
    44701 cattatgcgc cgactgccgg atgctgccta cgaagggcaa actacccacg aacgttaaac
    44761 accgcatacg tgttattaag caacacttcg gcaatctcat ccgcgggttc cgggcgtaac
    44821 tcgcaaagca cggcaaacac acgggcagcc tgttccgggc ggttaggctg cccctgaaaa
    44881 ccgttgagcg gcatatccgg cgcatcggtt tccagcaaca acgatgccag cggtaatttt
    44941 gcgatgacat cgcgggtttt actggcgcgt ggataggtga tagtaccgcc tacgccgatt
    45001 ttgtagccca gctgtacaaa ccgttcagcc tgttgctggc tgccagaaaa tccgtggaca
    45061 acaccagcac gcggcaaatc gtggcgttta agatgcatcg ccagtttgtc gtgcgtgcgc
    45121 cgtgaatgca ggatcaccgg cagatcgtag cgtttcgcca gtttcagttg ttcgtcgagt
    45181 aaccactgct gcctctcaaa ttgcggatcg tcgccgaaga gatccagacc gatctccccc
    45241 accgccacca ccttcgccgg acgcctttcc agcgcctgct gtagctgctc aagagacaca
    45301 tcgctatgtt tttccaacat accaggatgc aagcccaata cggcatacag cggttgataa
    45361 ttttccgcta acgcctgcac acgggcaaaa ttttccgcct ctgtcgccgg aacaatgatc
    45421 ttgcctacgc ccgcttgtgc cgcgcgttgc aggctggcct cttcatcgcc aataaaaggc
    45481 gggaaatcaa aatggcagtg agtgtcgata aaacggcaaa tcaagccaga tcctcattgt
    45541 taaatgtcgg gtcgttcgcc tgcggagcgt cgctcacttc ggcaaccagc gtatcgttgg
    45601 cgactggcgc aggcggaatg acaatcgatt ccggcgtcac cactggcacc agatggcggg
    45661 taagcggcgc tttttcagtt aatagcttgc caaccgtggc gaggaaataa cgaccgcata
    45721 aacgcccaag tttatagtct tcacgcagcg ccggaatccg actgccaagc gcgatactat
    45781 gtaatggctt cggcggataa atttcggata tccgcagctt gcccggtggt ttctcaataa
    45841 attgctgaat gtcacgatag ctggtttcat gatgctgcac cagattgacc agcggctgca
    45901 ggctactgtc acccagccag cgttccatgc gtttgaacca ctgcggcgtg tagtacattt
    45961 gtgacggcac agtgcggatg acgaccaacg ttttagcgcc ctgccttgcc gcctctttaa
    46021 ccggaatcgc atcactgatc ccgccatcca ggtagttaat gccttccagc gacactccgc
    46081 tacgataaaa gccaggtatc gcactggagg cgcgaatcac atccagccag ttttgtttgg
    46141 ttggtaaaaa gtaattcggc gcgtagtcat cctgacgaca ggcgcacata taaaacgatt
    46201 tgccgctgtc aaacaaccgc gcggcggtat ccatttgcaa cggcatctgg cttgcggtgg
    46261 cctccaccag ccagtcgaga tcgataagat ttcctccacg gacaaagcgc aatggatcga
    46321 aaaattcgcg ttttgtggta tagcgcatga tgactttgcg cgcgtaaccg ggctgattgc
    46381 agataaacgc cgagaggttc tgcgccccgg cagatgtgcc gagataaaga tcgaaaggat
    46441 taaactgcgc gcgcataaac tcatccagca cgccagccgt gaaaattcca cgctgtccgc
    46501 cgccttcgca caccagagct attcgtccag gctggaacgg ccttagcgac aacggcgcaa
    46561 tattaccaag cgttacaggt attcgctgcc ccacttctgc cttcctgttt ttgttgtgaa
    46621 agaaagacaa agtaacgcaa tttattattc gctaaaaccg caaaagccag tccgctggac
    46681 tggctttgga taaaattttc ttaatggtct gaaatctagg gtcgttttcg gcccatgaac
    46741 aaactcacta ggaacagaat aatcccgacg acaaagacaa ttttagctgc gcctgcagcg
    46801 gtaccggcca gaccaccaaa cccaagtgcg gcggcgatta acgcgataac cagaaatatg
    46861 atgccccaac gaaacataag cgtctccttt accatagtta atgtcaccgc ttgatatgag
    46921 cgtgaaaatc actcaatgcg atatttttag tgtggtgcac atcgcgcctc ccgacaaagt
    46981 tcgggaggac gaattactta gttttcagat catttttaac gcttttcaca ccatctaccg
    47041 ctttggcgat actttcagca cggtcacttt gtgcctgaga atctacggta ccggagagct
    47101 gaaccacgcc atcggtggtt tcaactttca ctttacgtga agggacgata tcgtccgcca
    47161 gcagtttggc tttgatttca ctggtggtgg cggtgtcacc cgcgtagccc ttcaccgagc
    47221 cttctttagc gtcgcgaacg tgcagtttgt cgctgacaga ggtcacgcct tcaacgcctt
    47281 tcgccacttt cactgcctct tcggcctggg cctgactttc aacgaaaccg ctcagggtca
    47341 cgactttttg atcggttttg acagagatat cggtgctctt gatgttgtca tgatccacca
    47401 gcgccgcctt cactttcgcg gtgatggcgc tgtcatccat gaaattaccg actttattca
    47461 tagagctatc gactttttgc cctgcgcttt cattggtagt ctgcgcgttg ttttccgcgt
    47521 aggcagagcc ggtcgcgacg gcagaggtca acattacagc cagcagagtt ttcgaaatct
    47581 tcagtcttgt catagtcatc gatttattcc tgtatgtttg ctcgtaattt gagcctagca
    47641 acacgaggtt gcattgctga atagggagag acttcaccct ctacagaagt caatggtcgc
    47701 catcacaaaa gcgatgggtg atgaataacg accattacag cctctgaatc agttattaat
    47761 atcggtagaa tgacaatcga cggctttaga tactaatatc tacgcattga acggtattta
    47821 acgccatcag aaatgtcatc actttgttaa atatagatca caattttgaa accgctcggg
    47881 atatcagcga aaacataagc aaaagtgaat gttttaagaa cattccgtaa gcggctaata
    47941 aggaagggaa attgacaggg cgcagcggct accgcgccct ggaggcaaga attaatgctc
    48001 gcgggtctgg tggaactgaa cgtccggata acgttcctgt gccaggcgca ggttgaccat
    48061 gctggtagcg atgtaagcga ggttatcgcc gccatcaagc gccagttggc tttcgttctt
    48121 acgcttgaac tcttcgaatt tcttcgcgtc tgcacattct acccagcggg cagtggcaac
    48181 gttgactgac tcatacactg cttcaacgtt gtattcgctc ttcaggcgcg ctaccaccac
    48241 atcaaactgc agcacaccaa ccgcaccaac gatcagatcg ttgttggaga ttggacggaa
    48301 cacctgcacc gcgccctctt cggaaagctg taccagccct ttgagcagct gtttttgctt
    48361 cagcggatct ttcaggcgga tacgacggaa cagttctggt gcgaagttcg gaataccggt
    48421 gaacttcatc atctcaccct gggtaaaggt gtcgccgatc tgaatggtgc cgtggttgtg
    48481 caggccgagg atatcgcccg gatacgcttc ttcaacgtgc gaacggtcac ccgccataaa
    48541 ggtcagcgcg tcggagatca ccacatcttt cgcagtgcgc acctggcgca gtttcatgcc
    48601 tttttcatat ttaccggaca ccacacgcat aaacgccacg cggtcgcggt gtttcggatc
    48661 catgttggcc tgaattttaa atacgaagcc ggtaaattta tcttcgctcg cttctacggt
    48721 acgggtatcg gtctgacgcg gcatcggcgc aggcgcccac tccaccaggc cattcagcat
    48781 atgatcaacg ccgaagttac ccagcgcagt accgaagaat accggagtga tttcgcccgc
    48841 aaggaacagc tctttgtcga actcgttaga cgcgccttta accagttcca gttcgtcacg
    48901 cagctgctgt gccagatctt caccaaccgc agcatcgaga tccgggttat tcagcccttt
    48961 aacaatgcgg acttcctgaa tggtgtgccc tttaccgctc tgatagagat aggtttcatc
    49021 tttataaagg tggtaaacgc ctttaaacag cttgccgcag ccaattggcc aagtgatcgg
    49081 tgcgcagcca attttcagct cgttctcaac ttcatcgagc agttccatcg ggtcgcggat
    49141 atcacggtca agtttgttca taaaggtgag gatcggcgtg tcgcgcagac gggtaacttc
    49201 catcagctta cgggtacgat cttcaacacc ttttgcggcg tcgataacca tcaggcagca
    49261 gtccaccgcc gtcagggtac gataggtatc ttccgagaag tcttcgtgcc ccggggtgtc
    49321 gagcaggtta accaggcaat cgtgatacgg aaactgcatc acagacgtag taatggagat
    49381 cccacgctgc ttttccatct ccatccagtc cgacttagcg tgctggttgg aaccacggcc
    49441 ttttactgta ccggcggtct gaatggcctg tccgaacagc agcaccttct cggtgatggt
    49501 agttttaccg gcgtccgggt gagaaataat ggcaaaagtg cggcgcttcg ccacctcttt
    49561 caaataagga gacaacgtca taattctttc ttcttgagta agcggcatcg cgccgcgctt
    49621 gttgaaaacg aaaaattgcg gctattttac ccatcaacgg gggggaggca atcagtgttt
    49681 acacaggagc tgctccagtt cgtgcaacga agaaacggtc caggtgggcg cgatgccttc
    49741 tggttgctcg cgattgtgcg cattcagcca gcaggtcgca agcccggcgt tgatgccacc
    49801 gagaatatcg gactcggcag tgtcgccaac catcagcacg cgtgaacggt caggattgcc
    49861 cgcctgttcc agcgcataat cgaaaatttt cttattcggt ttggcaacgc caacttcttc
    49921 ggaaatcacc agcaaatcga agtaatcacg caggcccgtc cgttccagac gcacttgctg
    49981 caaggcgcta aaaccgttgg tgatgatgcc gattttagcg ttgctacgaa tggcgttaag
    50041 cagagaaacc gcgcccggca acggcgtaca gatttccgcc atcgcattaa taaaggcatc
    50101 gttaagcaag cctgcttcaa ctttcagccg ttcggcccag ctctcgaaac gcccgtgctg
    50161 aagctgtaat gaagtgatcg cgccgttttg ataatccacc cacagtggct tgttaacggc
    50221 ctgatagtcc tgaaaatctt cagcggtaaa ggtgacgctg taatcaagaa acatccgctg
    50281 caggccggtg aatgagtcaa aggtaaacag cgtttcatcg gcatcaaaga aaatccagtc
    50341 ccacttcatt attccacctt gtcttacata ctgattggca acgccatgat gatggcgtct
    50401 tcgcgaccgt ccgtggtggg gtagtaattg cggcgaatcg tcgcctcgtt aaagcctaaa
    50461 ctttcgtaca gggcaatggc ggcagcattt gaggcacgga cttccagcca tagtgtcgcc
    50521 acgccgcgtt tttccagttc gtcgatcaga tgttccagca gcgccctacc cagcccctga
    50581 cgctgatagt cgggatcgac cgcaatattg aacaatgtag cttcatccag caccacttgc
    50641 gtaatcgcaa acgccgccat tttgccgttt tgcgttaact gaaagttgag ataacgctcg
    50701 ccctggttgc tggcaaaggt tttttcactc cacggaaagg cgtgggcgcg ttgttcaatg
    50761 tggtaagccg ctggtaaatc agtcgtttcg agggaagaaa tcgtgttcat atgtgcaaat
    50821 ttgttgccat aacgcggcgc gtgccgttgg gtttgcccgt aattcggtga gcgccggtga
    50881 tgccacctga gcgccttcca atgatagcgg ttcgtcagta cccaaccgcc agctgttgca
    50941 gcgactgcct tgcggcagca tcgcgatttt ttctggcgtc agttgcagca cttgatcggg
    51001 gctgacggtt aatgcgcgca gaacatcgct cactaaagga tcagtcaggg cgggaagatc
    51061 gtttgccacc atcaccagac ggacgtgtgc cgggctcgca atggcaatct ccccctgcaa
    51121 cgcgccaggg cgacgcagcg accactgggt aatgcccagt tgctgtaact gccagtctcg
    51181 tcgggatgtc atagcgaatc gctcctgttg tcaggggcgc aaatatagca aattcgtcga
    51241 taccgcgcca acatatggct ataatcgccg ccagtatcaa ttgaggagca ttccatgtct
    51301 gcatttaccc cggcaagtga agtcttgctg cgtcacagtg atgatttcga acaaagccgt
    51361 attctgtttg ccggagactt acaggatgac ctgcccgcgc gtttagatac cgcggccagc
    51421 cgtgctcata cccagcaatt ccaccattgg caggtgttaa gctgccagat gggggataac
    51481 gcccgtttta gtctggtcgc cacggcgaat gatgtcgcag attgcgatac gctgatttac
    51541 tactggccga agaacaaacc ggaagcccag ttccagttga tgaatttact ttctctgctg
    51601 ccagtgggga cagatatttt tgtcgttggc gagaaccgca gcggcgtgcg cagcgctgag
    51661 cagatgctgg cagattatgc gccgttgaat aaagtcgaca gcgctcgtcg ctgtggcctc
    51721 tattttggtc gtctggaaaa acagccagta tttgatgcgg ataaattctg gggcgaatac
    51781 agcgtcgatg gcctgacggt caaaacgctg cctggcgtgt ttagccgcga cggtctggat
    51841 gtcggtagcc agttgctgct ctcgacgtta actccgcaca cgaaaggtaa agtgctggat
    51901 gtcggctgtg gcgcgggtgt actttcagtt gcctttgcgc gccattcgcc gaaaattcgt
    51961 ctcaccttgt gcgatgtctc tgccccggcg gtagaagcca gccgcgcaac acttgcagcc
    52021 aacggtgttg aaggtgaagt ctttgccagc aacgtctttt ccgaggtgaa aggttgtttt
    52081 gatatgatca tctccaaccc gccgttccac gatgggatgc aaaccagcct ggatgcggcg
    52141 caaacgctga ttcgtggcgc ggtgcgtcat cttaatagcg gcggcgagct gcgaattgta
    52201 gcgaacgcct tcctgcctta cccggacgtg ctggatgaga catttggctt ccatgaagtt
    52261 atcgcgcaaa ccgggcgctt caaggtatat cgcgccatta tgacccgcca ggcgaagaaa
    52321 ggttgagtat ctgatatctg tcgccgcata ccgtgtcgga tgcggcgtga acgccttatc
    52381 cgacctacaa aacctgcacg ttagcccttc gtaggccaga taagacgcgc cagcgtcgca
    52441 tctggcatct gcccccgcat gcctaccaaa cctgcacgct agtttcctga tggacatttt
    52501 tccagcaatt acacatcagg cgataattaa ctattgacga aaagctgaaa accactagaa
    52561 tgcgcctccg tggtagcaat tctttttaag aattgatgg
//
LOCUS       DAZOYF010000010        50347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZOYF010000010 DAZOYF010000000
VERSION     DAZOYF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.010"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(111..1775)
                     /gene="glnS"
                     /locus_tag="ACHUOF_000589"
     CDS             complement(111..1775)
                     /gene="glnS"
                     /locus_tag="ACHUOF_000589"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR0609906.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1978..3924)
                     /gene="nagE"
                     /locus_tag="ACHUOF_000590"
     CDS             complement(1978..3924)
                     /gene="nagE"
                     /locus_tag="ACHUOF_000590"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR0609907.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4257..5057
                     /gene="nagB"
                     /locus_tag="ACHUOF_000591"
     CDS             4257..5057
                     /gene="nagB"
                     /locus_tag="ACHUOF_000591"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR0609908.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5117..6265
                     /gene="nagA"
                     /locus_tag="ACHUOF_000592"
     CDS             5117..6265
                     /gene="nagA"
                     /locus_tag="ACHUOF_000592"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR0609909.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6274..7494
                     /gene="nagC"
                     /locus_tag="ACHUOF_000593"
                     /gene_synonym="nagR"
     CDS             6274..7494
                     /gene="nagC"
                     /locus_tag="ACHUOF_000593"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR0609910.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7542..8294
                     /gene="nagD"
                     /locus_tag="ACHUOF_000594"
                     /pseudo
     CDS             7542..8294
                     /gene="nagD"
                     /locus_tag="ACHUOF_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            complement(8345..8443)
                     /locus_tag="ACHUOF_000595"
                     /pseudo
     CDS             complement(8345..8443)
                     /locus_tag="ACHUOF_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8691..10355
                     /gene="asnB"
                     /locus_tag="ACHUOF_000596"
     CDS             8691..10355
                     /gene="asnB"
                     /locus_tag="ACHUOF_000596"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR0609911.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10734..10810
                     /locus_tag="ACHUOF_000597"
     tRNA            10734..10810
                     /locus_tag="ACHUOF_000597"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10768..10770,aa:Met,seq:cat)
     gene            10819..10903
                     /locus_tag="ACHUOF_000598"
     tRNA            10819..10903
                     /locus_tag="ACHUOF_000598"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10853..10855,aa:Leu,seq:tag)
     gene            10927..11001
                     /locus_tag="ACHUOF_000599"
     tRNA            10927..11001
                     /locus_tag="ACHUOF_000599"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10959..10961,aa:Gln,seq:ttg)
     gene            11036..11110
                     /locus_tag="ACHUOF_000600"
     tRNA            11036..11110
                     /locus_tag="ACHUOF_000600"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11068..11070,aa:Gln,seq:ttg)
     gene            11126..11202
                     /locus_tag="ACHUOF_000601"
     tRNA            11126..11202
                     /locus_tag="ACHUOF_000601"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11160..11162,aa:Met,seq:cat)
     gene            11251..11325
                     /locus_tag="ACHUOF_000602"
     tRNA            11251..11325
                     /locus_tag="ACHUOF_000602"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11283..11285,aa:Gln,seq:ctg)
     gene            11363..11437
                     /locus_tag="ACHUOF_000603"
     tRNA            11363..11437
                     /locus_tag="ACHUOF_000603"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11395..11397,aa:Gln,seq:ctg)
     gene            complement(11591..12766)
                     /gene="ubiF"
                     /locus_tag="ACHUOF_000604"
     CDS             complement(11591..12766)
                     /gene="ubiF"
                     /locus_tag="ACHUOF_000604"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR0609912.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12912..14336
                     /gene="miaB"
                     /locus_tag="ACHUOF_000605"
     CDS             12912..14336
                     /gene="miaB"
                     /locus_tag="ACHUOF_000605"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR0609913.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14489..15529
                     /locus_tag="ACHUOF_000606"
     CDS             14489..15529
                     /locus_tag="ACHUOF_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR0609914.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15526..15993
                     /gene="ybeY"
                     /locus_tag="ACHUOF_000607"
     CDS             15526..15993
                     /gene="ybeY"
                     /locus_tag="ACHUOF_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR0609915.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16083..16961
                     /gene="corC"
                     /locus_tag="ACHUOF_000608"
     CDS             16083..16961
                     /gene="corC"
                     /locus_tag="ACHUOF_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR0609916.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16986..18524
                     /gene="lnt"
                     /locus_tag="ACHUOF_000609"
     CDS             16986..18524
                     /gene="lnt"
                     /locus_tag="ACHUOF_000609"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR0609917.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18921..19829
                     /gene="gltI"
                     /locus_tag="ACHUOF_000610"
     CDS             18921..19829
                     /gene="gltI"
                     /locus_tag="ACHUOF_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR0609918.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19999..20739
                     /gene="gltJ"
                     /locus_tag="ACHUOF_000611"
     CDS             19999..20739
                     /gene="gltJ"
                     /locus_tag="ACHUOF_000611"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR0609919.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20739..21413
                     /gene="gltK"
                     /locus_tag="ACHUOF_000612"
     CDS             20739..21413
                     /gene="gltK"
                     /locus_tag="ACHUOF_000612"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR0609920.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21413..22138
                     /gene="gltL"
                     /locus_tag="ACHUOF_000613"
     CDS             21413..22138
                     /gene="gltL"
                     /locus_tag="ACHUOF_000613"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR0609921.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22256..23191
                     /gene="rihA"
                     /locus_tag="ACHUOF_000614"
     CDS             22256..23191
                     /gene="rihA"
                     /locus_tag="ACHUOF_000614"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR0609922.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            23275..24945
                     /gene="hscC"
                     /locus_tag="ACHUOF_000615"
                     /pseudo
     CDS             23275..24945
                     /gene="hscC"
                     /locus_tag="ACHUOF_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(25005..26456)
                     /gene="djlC"
                     /locus_tag="ACHUOF_000616"
     CDS             complement(25005..26456)
                     /gene="djlC"
                     /locus_tag="ACHUOF_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR0609923.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEEESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(26453..27160)
                     /locus_tag="ACHUOF_000617"
     CDS             complement(26453..27160)
                     /locus_tag="ACHUOF_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0609924.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            27262..27816
                     /locus_tag="ACHUOF_000618"
     CDS             27262..27816
                     /locus_tag="ACHUOF_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0609925.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(27826..29253)
                     /locus_tag="ACHUOF_000619"
     CDS             complement(27826..29253)
                     /locus_tag="ACHUOF_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR0609926.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(29250..29957)
                     /locus_tag="ACHUOF_000620"
     CDS             complement(29250..29957)
                     /locus_tag="ACHUOF_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0609927.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            30121..31098
                     /gene="ybeQ"
                     /locus_tag="ACHUOF_000621"
     CDS             30121..31098
                     /gene="ybeQ"
                     /locus_tag="ACHUOF_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR0609928.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(31168..31650)
                     /locus_tag="ACHUOF_000622"
     CDS             complement(31168..31650)
                     /locus_tag="ACHUOF_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR0609929.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            31885..34467
                     /gene="leuS"
                     /locus_tag="ACHUOF_000623"
     CDS             31885..34467
                     /gene="leuS"
                     /locus_tag="ACHUOF_000623"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR0609930.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            34482..35063
                     /gene="lptE"
                     /locus_tag="ACHUOF_000624"
     CDS             34482..35063
                     /gene="lptE"
                     /locus_tag="ACHUOF_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR0609931.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            35063..36094
                     /gene="holA"
                     /locus_tag="ACHUOF_000625"
     CDS             35063..36094
                     /gene="holA"
                     /locus_tag="ACHUOF_000625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR0609932.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            36096..36737
                     /gene="nadD"
                     /locus_tag="ACHUOF_000626"
     CDS             36096..36737
                     /gene="nadD"
                     /locus_tag="ACHUOF_000626"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR0609933.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            36761..37372
                     /locus_tag="ACHUOF_000627"
     CDS             36761..37372
                     /locus_tag="ACHUOF_000627"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFR0609934.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            37632..37949
                     /gene="rsfS"
                     /locus_tag="ACHUOF_000628"
     CDS             37632..37949
                     /gene="rsfS"
                     /locus_tag="ACHUOF_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR0609935.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            37953..38420
                     /gene="rlmH"
                     /locus_tag="ACHUOF_000629"
     CDS             37953..38420
                     /gene="rlmH"
                     /locus_tag="ACHUOF_000629"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR0609936.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            38451..40352
                     /gene="mrdA"
                     /locus_tag="ACHUOF_000630"
     CDS             38451..40352
                     /gene="mrdA"
                     /locus_tag="ACHUOF_000630"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR0609937.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            40355..41467
                     /gene="mrdB"
                     /locus_tag="ACHUOF_000631"
                     /gene_synonym="rodA"
     CDS             40355..41467
                     /gene="mrdB"
                     /locus_tag="ACHUOF_000631"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR0609938.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            41478..42566
                     /gene="rlpA"
                     /locus_tag="ACHUOF_000632"
     CDS             41478..42566
                     /gene="rlpA"
                     /locus_tag="ACHUOF_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR0609939.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            42705..43916
                     /gene="dacA"
                     /locus_tag="ACHUOF_000633"
     CDS             42705..43916
                     /gene="dacA"
                     /locus_tag="ACHUOF_000633"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR0609940.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            44027..44290
                     /gene="ybeD"
                     /locus_tag="ACHUOF_000634"
     CDS             44027..44290
                     /gene="ybeD"
                     /locus_tag="ACHUOF_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR0609941.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            44391..45032
                     /gene="lipB"
                     /locus_tag="ACHUOF_000635"
     CDS             44391..45032
                     /gene="lipB"
                     /locus_tag="ACHUOF_000635"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR0609942.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            45291..46244
                     /locus_tag="ACHUOF_000636"
     CDS             45291..46244
                     /locus_tag="ACHUOF_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR0609943.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            46453..47418
                     /gene="lipA"
                     /locus_tag="ACHUOF_000637"
     CDS             46453..47418
                     /gene="lipA"
                     /locus_tag="ACHUOF_000637"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR0609944.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(47519..47722)
                     /gene="tatE"
                     /locus_tag="ACHUOF_000638"
     CDS             complement(47519..47722)
                     /gene="tatE"
                     /locus_tag="ACHUOF_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR0609945.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(47851..48639)
                     /locus_tag="ACHUOF_000639"
     CDS             complement(47851..48639)
                     /locus_tag="ACHUOF_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR0609946.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            48732..49115
                     /gene="crcB"
                     /locus_tag="ACHUOF_000640"
     CDS             48732..49115
                     /gene="crcB"
                     /locus_tag="ACHUOF_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR0609947.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(49169..49378)
                     /gene="cspE"
                     /locus_tag="ACHUOF_000641"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(49169..49378)
                     /gene="cspE"
                     /locus_tag="ACHUOF_000641"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0609948.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(49553..50113)
                     /gene="pagP"
                     /locus_tag="ACHUOF_000642"
     CDS             complement(49553..50113)
                     /gene="pagP"
                     /locus_tag="ACHUOF_000642"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR0609949.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT        12640 a        12828 c        12615 g        12264 t
ORIGIN      
        1 atgtaggcct gataagcgaa gcgcatcagg caatttagcg tttgaaattt attgtctatt
       61 tattgccgga tgcgacgtaa atgcctcacc cggcttggta gaacttaaaa ttactcgcct
      121 actttcgccc aggtatcacg cagcccaacg gtgcggttaa ataccggttt ttcggccgtg
      181 gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa actggaatgc tttacccgca
      241 accgcatctt tcagcgacgg ttcagcaaag ccctgtttga tcaccagcga ttccgggtta
      301 atcaccgaca ggaaatcatc cgcagcacct gggttaggca cgctgaacag acgatcatac
      361 aaacggattt caaccggcag cgcatgtgcc gcgctcaccc agtgaataac acctttgact
      421 ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt cataagtaca gaagatggtg
      481 gtgatattac cttcggcatc tttctccacg cgttcagcct taatgacgta agcattacgc
      541 agacgcactt ctttacccag caccagacgt ttgtactgct tgttagcttc ttcgcggaaa
      601 tcggcgcgat cgatccaaat ttcaccgcta aacggcacct gacggctccc catttccggt
      661 ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc cctgatagtt ttcgataacc
      721 agtttcaccg gatcgataac cgccattgcg cgcggcgcat tttcgttgag atcttcacgg
      781 atgcaggatt ccagcgacgc catctcaatg gtgttgtcct gcttggtcac gccgatgcgt
      841 ttgcagaact cacgaataga agccgcagtg taaccacgac gacgcagacc ggaaatggtc
      901 ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg tcaccagcag gttcagctta
      961 cgcttggaca tcacagtgta ttccagattc aggcgcgaga attcgtactg gcgcggatga
     1021 acaggaatgg tgatgttatc cagtacccag tcgtacagac gacggttgtc ctggaactca
     1081 agcgtacaca gagagtgcgt aataccttcc agcgcatcgc tgatgcaatg ggtgaagtcg
     1141 tacatcgggt agatgcacca cttgttgcca gtctggtggt gttcagcaaa tttaatgcgg
     1201 tacagcaccg gatcgcgcat tacgataaat ggcgacgcca tgtcgatttt cgcacgcagg
     1261 caggctttac cttcttcaaa accaccggca cgcatttttt cgaacagcgc caagttctct
     1321 tcaacgctgc ggtcgcggta cgggctgttt ttacccggct gcgtcagggt accgcggtat
     1381 tcgcggatct gttccggtgt cagttcatca acgtacgcca ggcctttatt gatcagttcg
     1441 atcgcatagg cgtggagctg atcaaaataa tcggaggagt aacggacgtt accagaccag
     1501 tgaaaaccta accactctac gtcgtttttg atcgaatcga catactcgat atcttctttt
     1561 accgggttag tgtcgtcgaa acgcaggttg cactggcctt tatagtcctg ggcgatcccg
     1621 aagttcaggc agatagattt cgcatggcca atatgcagat agccattcgg ctccggcggg
     1681 aaacgggtgt gtactgtgat gtgcttacca ctggccagat cttcatcgat gatctgacgg
     1741 ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg attcctcaaa gcgtaaacaa
     1801 cgtataacgg cgtatgatct tataagcggg acaggctgac aactgttagt tttttaaaat
     1861 tgataatcta acaaggatta tggggatgat tgatgaaaaa aaaagcggca gagaagatct
     1921 ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc atcaggcata aagcagatta
     1981 ctttttgatt tcatacagcg gtgtttgacc cgccacaaca tggccctgag ctttaatgag
     2041 caagccactg aaatcgtcga tattgctgca aaccaccggg ctaatcatcg agcgggcgtt
     2101 agcgttcagg taatccagat ccatttccag aatcggttgc cctgcgctta cctgcgcacc
     2161 ctcttccacc agacgtttaa agcctttacc ttccagcgct acggtgtcga tacccatatg
     2221 gacgacgatc tccgcgcctt tttcggtttc caggcagaac gcgtggttgg tgttgaagat
     2281 tttcacgatt gtccctgcgg ctggtgatac gacgatttta tctgtcggtt tcaccgccac
     2341 accgtcaccc accgctttgc tggcgaatgc ttcgtcagga acctgatcca gtgccacgac
     2401 atcaccggta atcggcgata ccagctccgc gatagatacc gcgtttggta cagcctgcgg
     2461 ttttgctaca ggcgcggcag ttgccggagt tgcttcagct gacgcagcgg ctaccggacc
     2521 acgggcaacg actttcttca tcgcatcgcc gatggattct gctttcgcgc caacaatcac
     2581 ctgaatagtc tgtttgttca gtttcactac accagaagca cccagacgtt tacacatcgt
     2641 atcgttgacg cgggcagagt cagccacggt caggcgcaga cgggtaatac aggcatcaat
     2701 cgctttcagg ttgtcagtac cgccaaccgc agcaatatag ttggttgcca gttgagtcag
     2761 accttcttca gtattgctgt tggcttcttc agtaacgatc tcgtcttctt tatcttcacg
     2821 acccggcgtt ttcaggttga acatgcggat aaccaaactg aacaccacga agtagatagc
     2881 gaagaagata acgcccatca ccagcagcat ccagacgttc tggctggcgg caggcaggtt
     2941 atacatcaac gcgtagtcga tagcacccgc agagaaagag aagccggcat ggatacccag
     3001 cagcgttgcc acaaacaggc tgataccggt cagcagtgcg tgcaggaggt acagcagcgg
     3061 cgcaaggaac atgaacagga attccagcgg ctcagtcaca ccggtcagga acgcagtaac
     3121 agcaacagaa agcagcatcc cgccaaccat cggacgacgc tctttcggtg ctgcgaagta
     3181 catcgccagc gccgcacccg gcagaccgaa catcatgatc gggaagaagc cggacatgaa
     3241 catccccgcg gtgccgtcac cagcgtagaa acggttgatg tcgccgtgga aaaccgtacc
     3301 cgccgcgttg gtgaattcac caatctggaa ccaggcgatg gtgttcagca cctgatgcag
     3361 accggttggg atcagcagac ggttgatgaa accaaagata ccggaaccca gcgcgcccgc
     3421 agaaacgatc cactcgccgc ctgcatggat agcgtgctgt accggcggcc agacgtaacc
     3481 aaaaatggcc gccagcacca gacagaagaa gccggtggcg atcggcacaa agcgtttgcc
     3541 gccgaagaag ctcaggaagt ccggcagttt aatatcggac caacggttat aggctgcgcc
     3601 accaaccaga ccggtaatga tacccgccag tacacccatg ttaatttctg ggttgatggt
     3661 caccatcgct ttggttaaca caaagtaacc taccgcaccc gccagcgccg ccgcacctgc
     3721 gctgtctttc gaccagctgg atgccacacc gatggcgaag attaatgcga ggttatcaaa
     3781 aattgcaccg cccgcctggg caataaacgc aacgttaagt aaatctggct gaccgaatcg
     3841 cagcaacagc gccgccaccg gcagcaccgc gatagggagc tgtaacgccc taccgagtcg
     3901 ctggaaaaaa cctaaaatat tcatcttatt ccccctacga gaaccctatt tggctcgttt
     3961 aaagccgtat ttttattttg ctgcaaattt tactgccgat gttctgtaat cagattgtta
     4021 gatcatctgc tacagagtgt gtgaaaattt aattcgcatc gcaaattaaa cgcgtgtctt
     4081 ttgtgagttt tgtcaccaaa tatcgttatc atcactccct tttactggct aaaccagaaa
     4141 acttatttta tcattcaaaa aatcaagtcg gattgacgcc tgtctgcgca aatccaggtt
     4201 acgcttaaag atgtctaatc cgccaacggc ttacatttta cttattgagg tgaataatga
     4261 gactgatccc cctgactacc gctgaacagg tcggcaaatg ggctgctcgc catatcgtca
     4321 atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt tgtactgggc ctgccgactg
     4381 gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat gcataaagca ggccaggtca
     4441 gctttaagca cgttgtcacc ttcaacatgg acgaatatgt cggtctgccg aaagagcatc
     4501 cggaaagcta ctacagcttt atgcaccgta atttcttcga tcacgttgat attccagcag
     4561 aaaacatcaa ccttctcaac ggcaatgccc cggatatcga tgccgagtgc cgccagtatg
     4621 aagaaaaaat ccgttcttac ggaaaaattc atctgtttat gggcggtgta ggtaacgacg
     4681 gtcatattgc atttaacgaa ccggcgtctt ctctggcttc tcgtactcgt atcaaaaccc
     4741 tgactcatga cactcgcgtc gcgaactctc gtttctttga taacgatgtc aatcaggtgc
     4801 caaaatatgc cctgactgtc ggtgttggta cactgctgga tgccgaagaa gtgatgatta
     4861 tggtgctggg tagccagaaa gcactggcgc tgcaggccgc cgttgaaggt tgcgtgaacc
     4921 atatgtggac catcagctgt ctgcaactgc atccgaaagc gatcatggtg tgcgatgaac
     4981 cttccaccat ggagctgaaa gttaagactt taagatattt caatgaatta gaagcagaaa
     5041 atatcaaagg tctgtaattg ttatccctgc cctggttcct tgctcagggc aatatttttt
     5101 aaaatcgggg ggcagaatgt atgcattaac ccagggccgg atctttaccg gccacgaatt
     5161 tcttgatgac cacgcggttg ttatcgctga tggcctgatt aaaagcgtct gtccggtagc
     5221 ggaactgccg ccagagatcg aacaacgttc actgaacggg gccattctct cccccggttt
     5281 tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt aacgacaccg ctgaagcggt
     5341 cagcgtggaa acgctggaaa tcatgcagaa agccaatgag aaatcaggct gtactaacta
     5401 tctgccgacg cttatcacca ccagcgatga gctgatgaaa cagggcgtgc gcgttatgcg
     5461 tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg catctggaag gtccgtggct
     5521 gaatctggta aaaaaaggca cccataatcc gaattttgtg cgtaagcctg atgccgcgct
     5581 ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa gtgaccctgg caccggaaat
     5641 ggttcctgcg gaagtcatca gcaaactggc aaatgccggg attgtggttt ctgccggtca
     5701 ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc gcggggatta cctttgccac
     5761 ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa ccgggcctgg cgggcgcgat
     5821 cctcgacgaa gctgacattt attgcggtat tatcgctgat ggcctgcatg ttgattacgc
     5881 caacattcgt aacgctaaac gccttaaagg cgacaaactg tgtctggtta ccgacgccac
     5941 cgcgccagca ggtgccaaca ttgaacagtt catttttgcg ggtaaaacaa tatactaccg
     6001 taacggactt tgtgtggatg agaacggtac gttaagcggt tcatccttaa ccatgattga
     6061 aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg gatgaagtgc tgcgtatggc
     6121 gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt ctcggcacgc tcgccgcagg
     6181 taaagtagcc aacctgaccg cattcacacc tgattttaaa atcaccaaga ccatcgttaa
     6241 cggtaacgag gtcgtaactc aataagagaa agtatgacac caggcggaca agctcagata
     6301 ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg tttatcgcct gattgaccag
     6361 tacgggccaa tctcgcggat tcagattgcc gagcaaagcc agcttgcccc cgccagcgta
     6421 accaaaatta cgcgtcagct tatcgaacgc gggctgatca aagaagttga tcagcaggcc
     6481 tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa cccgcaattt ccacgcaatc
     6541 ggcgtacggc ttggtcgtca tgacgccacc atcactctgt ttgatctcag cagcaaagtg
     6601 ctggcagaag aacattaccc gctgccggaa cgtacccagc agacgctgga acatgccctg
     6661 ctgaatgcca ttgctcagtt tattgatagc taccagcgca agctgcgcga gctaatcgcc
     6721 atttcggtga tcctgccagg gcttgttgac ccggacagcg gcaaaattca ttacatgccg
     6781 catattcagg tagaaaactg ggggctggta gaagcactgg aagaacgttt taaagtgacc
     6841 tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg agcactactt cggtgcaagt
     6901 caggattgcg aagactccat tctggtgcgt gtccatcgcg gaaccggggc cgggattatc
     6961 tctaacgggc gcatttttat tggccgcaac ggtaacgtcg gtgaaattgg ccatattcag
     7021 gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg gctgcctgga aactatcgcc
     7081 gccaacgctg ccattgaaca acgggtgttg aatctgttaa agcagggcta ccagagccgt
     7141 gtgccactgg acgactgcac catcaaaact atctgcaaag ccgcgaacaa aggcgatagc
     7201 ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg gtaaaaccat cgccattgct
     7261 atcaacttat ttaatccgca aaaaattgtt attgctggtg aaatcaccga agccgataaa
     7321 gtgctgctcc ctgctattga aagctgcatt aatacccaag cgctgaaggc gtttcgtact
     7381 aatctgccgg tggtacgttc tgagctggat caccgctcgg caatcggcgc ttttgcgctg
     7441 gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt tgctggaaaa ttaatgtgct
     7501 tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc catgaccatt aaaaatgtaa
     7561 tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc cgtaccgggt gcagcggaat
     7621 ttttgcacgg gattatggat aaaggcctgc cgctggtgtt gctgaccaac tatccttcgc
     7681 agactgggca agatctggcg aaccgctttg ccactgccgg tgtcgatgtg ccggatagcg
     7741 tgttttatac ctctgcgatg gcgaccgccg atttcctgcg tcgctaggaa ggcaagaaag
     7801 cgtatgtggt gggcgaaggc gcactgattc atgaactgta caaagccggt ttcactatta
     7861 ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg ttcctacaac tgggacatga
     7921 ttcataaagc agcctatttc gtcgctaacg gtgcacgctt tatcgccacc aacccggaca
     7981 cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg tgcagggatt gagaaaatct
     8041 ccgggcgcaa accgttctat gttggtaagc ccagcccgtg gatcatccgc gcggcattaa
     8101 acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg cgataacctg cgtaccgata
     8161 ttctggcggg cttccaggcc ggtctggaga cgattctggt gctttctggt gtttcatcgc
     8221 tcgacgatat cgacagtatg ccgttccgcc caagctggat ttacccgtca gtcgctgaaa
     8281 tcgacgttat ctgaatacca cccacgtctg cggacgtggt tggctctgat gacaaacgcc
     8341 aaactgcctg atgcgctacg cttatcaggc ctacgcagct cctgcaatat attgaatttt
     8401 catgcttttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca aatcgcactt
     8461 tatcagcaat ttggcccccg taaaatataa ccattcaata aaacccgcta aaaattatcg
     8521 attcatcaat aaatacatcc tcacaccaga ccctttttca ccaatcgaca atcaccatta
     8581 cgttttttat ttttgcactg ccaaatagct tgcgcacagt gctgctttgc ggcatttttt
     8641 taaacaagca aacacaacaa gcaacaaata ccaggttaac ggagaaggtt atgtgttcaa
     8701 tttttggcgt attcgatatc aaaacagacg cagttgagct gcgtaagaaa gcactcgagc
     8761 tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg tatttatgcc agcgataacg
     8821 ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa cgcgggggcg caacctctct
     8881 acaaccaaca aaaaaaccac gtactggcgg taaacggtga aatctacaac caccaggcat
     8941 tgcgcgctga atatggcgat cgttatcagt tccagacagg gtctgactgt gaagtgatcc
     9001 tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga cttgcagggc atgtttgcct
     9061 ttgccctgta cgacagcgaa aaagatgcct acctgattgg tcgcgaccat ctggggatca
     9121 tcccactgta tatggggtat gacgaacacg gtcagctgta tgtggcctca gaaatgaaat
     9181 cgctggtgcc agtttgccgc acgattaaag agttcccggc ggggagctat ttgtggagtc
     9241 aggacggcga aatccgttct tattatcatc gcgactggtt cgactttgat gcagtgaaag
     9301 ataacgtgac cgacaaaaac gagctgcgtc aggcactgga agattccgtt aaaagccatc
     9361 tgatgtctga tgtgccttac ggtgtgctac tttctggtgg tctggattcc tcaattattt
     9421 ccgctatcac caagaaatac gcagcccgtc gcgtggaaga tcaggaacgc tctgaagcct
     9481 ggtggccgca gttacactcc tttgctgtag gtctgccggg ttcaccggat cttaaagcag
     9541 cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga aattcacttc actgtacagg
     9601 aaggtctgga tgccatccgc gacgtgattt accacatcga aacttatgat gtgaccacta
     9661 ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat caaggcgatg ggcattaaaa
     9721 tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg ttatctttac ttccataaag
     9781 cgcccaacgt taaagaactg catgaagaga cggtgcgtaa actgctggcc ttgcatatgt
     9841 atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg cgtggaagca cgcgttccgt
     9901 tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa cccgcaggat aaaatgtgcg
     9961 gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt tgagtcatac ctgcccgcaa
    10021 gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt cggttacagt tggatcgaca
    10081 ccctgaaaga agtggcggcg cagcaggttt ctgatcagca actggaaact gcccgcttcc
    10141 gcttcccgta caacacgccg acctcaaaag aagcgtatct gtaccgcgag atctttgaag
    10201 aactgttccc acttccgagt gccgctgagt gcgtgccggg cggtccttcc gtcgcgtgtt
    10261 cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa aatggacgat ccgtctggtc
    10321 gtgcggttgg cgttcaccag tcggcgtata agtaagaaat gcggtactgc cccggagaaa
    10381 tccggggctg ttttagttat tgcgctgctt gttgattctg atatgcagta aggaaatgtt
    10441 ccgggtttgc cacatcactg tttgacaact catcactgta ggccagtatt tcatagatta
    10501 atttcccacc actattgttg aattccgaat ttcatcctgt acgacaaaag aatggctgta
    10561 gggtggggaa aaacacaaag taaacaataa ttgacgaata tagcgccacg ctgttcgcaa
    10621 cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa agaggttgac gctgcaaggc
    10681 tctatacgca taatgcgccc cgcaacgccg ataaggtatc gcgaaaaaaa gatggctacg
    10741 tagctcagtt ggttagagca catcactcat aatgatgggg tcacaggttc gaatcccgtc
    10801 gtagccacca tcttttttgc gggagtggcg aaattggtag acgcaccaga tttaggttct
    10861 ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca ccattcacca gaaagcgttg
    10921 tacggatggg gtatcgccaa gcggtaaggc accggttttt gataccggca ttccctggtt
    10981 cgaatccagg taccccagcc atcttcttcg agtaagcggt tcaccgcccg gttattgggg
    11041 tatcgccaag cggtaaggca ccggtttttg ataccggcat tccctggttc gaatccaggt
    11101 accccagcca tcgaagaaat aatctggcta cgtagctcag ttggttagag cacatcactc
    11161 ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac caaattctga atgtgtcgaa
    11221 tacgttcggc aaattcaaaa accaatttgt tggggtatcg ccaagcggta aggcaccgga
    11281 ttctgattcc ggcattccga ggttcgaatc ctcgtacccc agccaattta ttcaagacgc
    11341 ttacatcgta agtgcaccca gttggggtat cgccaagcgg taaggcaccg gattctgatt
    11401 ccggcattcc gaggttcgaa tcctcgtacc ccagccacat taaaaaagct cgcttcggcg
    11461 agctttttgc ttttctgcgt tcattcaatg tcgaatgcga tgttgacacg tcttatcctt
    11521 caatgtcgga tgcgacgctg ccgcgtctta tccgacctac ggctggcacg catccggcaa
    11581 tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg acgtttcaac acgccagcac
    11641 gctctgccgc cattaacccg agattacgca caaaacgcag cggcggcaga ttattactga
    11701 atccggcata aaacagatcc ataccacttt gcataatgaa gttatccgcc atgcgccgca
    11761 tctggtaacg cttgaggaca ggataactgg cccacgcttc accgtagctg cgggcattca
    11821 ccagaacatc aatcagggca tcgacatcac gataaccaag attcaccccc tgccccgcca
    11881 gcggatggat ggtatgcgcg gcatcgccca ccagcgcaag ccccggctgc acatactgca
    11941 acgcatgtcg gcgcgtcagc ggaaacgcac cagcggcaag cggtgtaacg taacccagac
    12001 gcgacgggaa atgcttcgcg atttccgcct ggagttgcgc catattcata ttctgcaact
    12061 ggcgaatacg cgtcggagag tcataccaca ccagcgatgc ccagttatca aacaacggca
    12121 gaaacgcacg cggtccgtcc ggggtaaatt gctgccaggt gctgtcgccg ggatcgttct
    12181 cacactgtac gctaatcaac atgcacgact gcgcatactg ccaggcatga acgccaattc
    12241 ccgccatctg ccgcacctga gaatttgcgc cgtcggcacc aatcaccagt ttcgcgcgga
    12301 tcgtttcacc gcccttcagt tccagctcct gaagatcatt atctcgatgt aatgcaatca
    12361 gcgaggttgg cacacgtaac gttactttcg gatgcgcttc cagcgcctgc cacaacgcct
    12421 gttgcagcac tgtgttttcc accatgtagc caagcaacgg caactttaat tcggctgcgt
    12481 caaataccac atgtgccgtt tcccactccc aggtttccag tctgcggtaa ggatggcaac
    12541 gcatagcctg tactgcatcc cagaccccta accctttaag caatgatacc gaagccgcgc
    12601 tgatcgccga gatccgcacg tccggttggc tgtcagcgac aaacggtgca ggttctgcgt
    12661 gctcgatcac cgttaccgaa aatccgtgct gtgccagccc cagcgccagt gcgccgccga
    12721 ccattcctcc gccgacaatg gcaatttccg ttggttgatt tgtcatggtc aatcatcctg
    12781 ttagcaatat gctttaagtt tacaggattt ttacccccaa gggctgataa cgctcatact
    12841 ggtcacaacg gcagcaaagc attacactat gcgccctgca ttcctggcta ctatttcgca
    12901 agagcaagtc gatgaccaaa aaactccata ttaaaacctg gggctgtcag atgaacgagt
    12961 acgattcatc gaagatggcc gatctgctgg atgccaccca cggctatcaa ctgaccgacg
    13021 tggcggaaga agcggatgtg ctactgctga acacctgctc aatccgcgag aaggctcagg
    13081 aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa agagaagaat ccagacctga
    13141 ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga gcacattcgc cagcgcgccc
    13201 actatgtcga tattattttt gggccgcaaa cgctgcaccg tctgccggag atgatcaact
    13261 ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt cccggaaatc gagaagtttg
    13321 accgtctgcc ggaaccgcgc gccgaagggc cgaccgcgtt tgtctccatc atggaaggct
    13381 gcaataaata ttgcacctac tgcgtggtgc cttacacccg tggtgaagag gtaagccgtc
    13441 cgtctgacga tattctgttt gagattgccc agcttgcggc tcagggcgtg cgtgaagtca
    13501 acctgctcgg tcagaatgtg aatgcctggc gtggtgagaa ctacgacggc accaccggat
    13561 cgtttgccga tctgttgcgt ctggttgctg cgatcgacgg gatcgatcgt attcgcttta
    13621 ccaccagcca tccgatcgaa ttcaccgacg atatcatcga agtgtaccgc gacacgcctg
    13681 agctggtgag cttcctgcat ctgccggtac agagcggttc cgatcgcatt ctgaacctga
    13741 tgggacgtac ccatacggcg ctggagtaca aagcgatcat ccgtaaactg cgtgcggcgc
    13801 gtccggatat tcagatcagt tctgacttca ttgttggctt ccctggcgaa accaccgaag
    13861 acttcgagaa aacgatgaag ctgattgccg acgtcaattt cgacatgagc tacagcttta
    13921 tcttctctgc acgtccagga acgcctgccg ccgatatggt tgatgatgtt ccggaagaag
    13981 agaagaagca gcgtctgtat attctgcaag aacgtatcaa tcagcaagcg atggcatgga
    14041 gccgtcggat gctcggcacc acccagcgta ttctggtaga agggacttcg cgtaagagca
    14101 tcatggagct ttccgggcgt accgaaaata accgcgtggt caacttcgag ggcacaccgg
    14161 atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt ctacccgaac tctctgcgcg
    14221 gtaaagtggt gcgaactgaa gatgaaatgg gtctgcgcat ggcagaaaca ccggagtcgg
    14281 tgattgcccg cacccgcaaa gaaaacgacc ttggcgtggg ttattatcag ccgtaattct
    14341 caggcctgcc gttccggtgg gccttgtttt ttctctcttt atccccatct ttatcgcaat
    14401 gcttgcgcct ttttcctgtg gcgtgaataa tttccttata ggccggtgaa tgttcagctt
    14461 ccggcggcat acagataaga ggaacggttt gaacatagac actcgcgaaa tcaccctgga
    14521 gccagcagac aatgcgcgtc tgttgagcct gtgcggcccg tttgatgaca acatcaagca
    14581 gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat aaccacttta aactgaccgg
    14641 ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt agcctgtatg tcgatactgc
    14701 cccgatgcgc ggtcagattc aggatatcga accggaacag atccaccttg cgattaaaga
    14761 agcgcgggta ctggagcaaa gcgcggagag cgtgccggag tacggcaaag cggtcaatat
    14821 caaaactaaa cgcggcgtaa ttaagccgcg cacgccaaac caggcgcagt acatcgccaa
    14881 tattctcgac catgacatta ccttcggcgt tggcccggcg ggtacgggta aaacctacct
    14941 ggcagtggct gcggcagttg atgccctgga gcgtcaggaa attcgccgta ttctgctgac
    15001 tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg cctggcgatt taagccagaa
    15061 agtagacccg tatctgcgcc cactgtacga cgcgctgttt gaaatgctgg gctttgagaa
    15121 agtcgagaaa ctgattgagc gcaacgttat tgaagtcgcg ccgctggcct atatgcgtgg
    15181 tcgtacgctg aacgacgcgt ttatcattct cgatgagagc cagaacacta ccatcgaaca
    15241 gatgaagatg ttcctgaccc gtatcggttt taactcaaaa gcggttatca ccggcgacgt
    15301 cacacagatc gacctgccgc gtaatactaa atcaggctta cgtcatgcca ttgaagtgct
    15361 ggccgatgtc gaagagatca gctttaactt cttccacagc gaagacgtgg ttcgtcaccc
    15421 ggtggtggcg cgtatcgtta acgcttatga agcctgggaa gaagcagaac aaaaacgtaa
    15481 agcggcgctg gcagcagaac gcaagcgcga agaacaggaa caaaaatgag tcaggtgatc
    15541 ctcgatttac aactggcatg tgaagataat tccgggttac cggaagagag ccagtttcaa
    15601 acatggctga atgcggtgat cccgcagttt caggaagagt cggaagtgac tattcgcgtg
    15661 gtcgataccg ccgaaagcca cagtctgaat ctgacctatc gcggtaagga taagccgacc
    15721 aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg agatgtcgct actgggcgat
    15781 ctggttatct gccgtcaggt ggttgagaag gaagctcagg agcaaggcaa accactggag
    15841 gcgcactggg cgcatatggt ggtgcacggc agtctgcatt tgttaggtta cgatcacatt
    15901 gaagatgacg aagcagaaga aatggaagcc ctcgaaacag agattatgct tgctctgggc
    15961 tatgaggatc cgtacattgc cgagaaagaa taatttgcca ggcatttgac tggcagacgt
    16021 ccccgccgtt ggtgattaac gacggcgttt tgattaacta acatgagaac ccatacgacg
    16081 ccatgagcga cgacaattca cacagtagtg acacgataag caacaagaag ggatttttct
    16141 ccctgttact cagccaactt ttccacggtg aaccgaaaaa ccgtgacgaa ctgctggcgc
    16201 tgatccgtga ttccgggcag aacgacctta tcgacgaaga tacgcgcgat atgctcgaag
    16261 gggtgatgga catcgcagac caacgcgttc gcgacatcat gatcccccgc tcccagatga
    16321 ttaccctgaa acgcaaccag acgctggacg aatgccttga tgtcatcatc gagtccgccc
    16381 actcacgttt cccggtgatt agcgaagaca aagatcacat tgaagggatt ctgatggcga
    16441 aagacttgct gccgtttatg cgcagcgatg ctgaagcctt cagcatggac aaagtgttac
    16501 gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg gatgctgaaa gagtttcgct
    16561 ctcagcgtta ccacatggcg atcgttattg acgaattcgg tggggtttcc ggtctggtga
    16621 ccattgaaga catcctggaa ctgattgttg gtgagattga agacgagtat gacgaagaag
    16681 atgatatcga cttccgtcag ctgagtcgtc atacctggac cgtgcgcgca ctggcttcca
    16741 ttgaagactt caacgaagcg ttcggcaccc actttagcga tgaagaagtc gacactatcg
    16801 gtggtctggt gatgcaggcg tttgggcatc ttccggcgcg tggcgaaact atcgacatcg
    16861 acggttacca gttcaaagtg gcgatggccg atagtcggcg tattattcag gttcatgtca
    16921 aaatcccgga tgactcaccc cagccaaagc tggatgaata aaaccgaaac tggatagata
    16981 actacatgga ttttgcctca ttaattgaac gccagcgcat tcgcctgctg ctggcgttat
    17041 tattcggtgc ctgcggaacg ctggccttct ctccttacga cgtctggcct gcggcgatta
    17101 tttcgctgat ggggcttcag gcgttgacct ttaaccgtcg cccactccag tctgccgcta
    17161 ttggcttttg ctggggattt ggcctctttg gcagcggtat taactgggtc tatgtcagca
    17221 tcgcgacctt tggcgggatg cctggcccgg ttaacatctt cctggtggtg ctgctggcgg
    17281 cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct gtcgcgtctg tggccgaaaa
    17341 ccacctggct gcgcgtggcg attgccaccc ctgctctctg gcaagtgacc gagtttctgc
    17401 gcggttgggt gctgactggc ttcccgtggt tacagttcgg ctatagccag attgatggcc
    17461 cgttaaaagg gctggcaccg ctaatgggcg tggaagccat taacttcctg ctgatgatgg
    17521 ttagtggcct gctggcactg gcgttggtca aacgcaactg gcgtccgctg gtggtggccg
    17581 tcgtgctgtt tgccctaccc ttcccgctgc gttacatcca atggtttacc ccgcaaccgg
    17641 agaaaaccat tcaggtttcg atggttcagg gcgatattcc gcaatcgctg aaatgggacg
    17701 aaggccagct tcttaatacg ctaaagattt actacaacgc aacggcaccg ctgatgggca
    17761 aatcatcgtt gattatctgg ccggagtcgg cgataaccga tctggaaatt aatcagcaac
    17821 cgttcctcaa agcactggac ggtgagttgc gtgataaagg tagctcgctg gtaaccggga
    17881 ttgtcgacgc gcgtctcaat aagcagaacc gctacgatac ctacaacacc atcatcacgc
    17941 tgggtaaagg tgcaccgtac agctacgaat cagccgatcg ctataacaaa aaccatctgg
    18001 tgccgtttgg cgagtttgtc ccgctggagt cgattctgcg tccgttagca ccgttctttg
    18061 atctgccgat gtcgtcgttc agccgtgggc catatatcca gccgccgctg tcggcaaatg
    18121 gtattgagct tactgcggct atttgctacg agattattct cgccgagcaa gtgcgcgata
    18181 acttccgccc ggataccgac tacctgctga ctatctccaa cgatgcgtgg tttggtaaat
    18241 ctattggtcc atggcaacac ttccagatgg cgcgaatgcg tgcgctggag ctggcgcgcc
    18301 cactgttgcg cagcaccaac aacggcatta cggcggtgat tggcccgcag ggtgagattc
    18361 aggcgatgat cccgcagttc gcccgcgagg tgttaaccac taacgtgacg ccgaccaccg
    18421 gactcacacc atacgcacgt accggcaact ggccgctgtg ggtgctgacg gcactgtttg
    18481 gttttgctgc tgtgttgatg agtctgcgtc agcgacgtaa ataatccctt cttatcgtgc
    18541 cggaatacat cccatatttc ggcacgctca ccggatgcgg catcagtgca ttatccggat
    18601 aacaatattc tcctatccaa ctttggcaca tctattgctt tgttatacaa ggcaaagctt
    18661 gaaccagcat cagtacaacc cggttgcacc acagcaatcc accggtagca ccaaaagagt
    18721 gcattttaag aattttgctt cagaatggtg cggtgcacct cgcaaaaaca aacaataaaa
    18781 catcaatatc tttacattta gccaaactaa atgttaccta acaggcacaa cactgcacaa
    18841 taaagttgca gacgataaca acacaaacac tcacaacggg tatccatgcg ttcttaacgc
    18901 agaagataaa ggtgttggat atgcaattac gtaaacctgc cacagcaatc ctcgccctgg
    18961 cgctttccgc aggactggca caggcagatg acgccgcccc ggcagcaggc agtacgctgg
    19021 acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg tgaatcttca gtacctttct
    19081 cttattacga caatcagcaa aaagtggtgg gttactcgca ggattactcc aacgccattg
    19141 ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca ggtaaaactg attccgatta
    19201 cctcacaaaa tcgtattcca ctgctgcaaa acggcacttt cgattttgaa tgtggttcta
    19261 ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc tgacactatt ttcgtggtcg
    19321 gtacgcgcct gttgaccaaa aagggcggcg atatcaaaga ttttgccgac ctgaaaggca
    19381 aagccgtagt cgtcacttcc ggcactacct ctgaagtttt gctcaacaaa ctgaatgaag
    19441 agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca cggtgactct ttccgcaccc
    19501 tggaaagcgg tcgtgccgtt gcctttatga tggatgacgc tctgctggcc ggtgaacgtg
    19561 cgaaagcgaa gaaaccagac aactgggata tcgtcggcaa gccgcagtct caggaagcct
    19621 acggttgcat gttgcgtaaa gacgatccgc agttcaaaaa gctgatggat gacactatcg
    19681 ctcaggtgca gacctccggt gaagcggaaa aatggtttga taaatggttc aaaaatccaa
    19741 ttccgccgaa aaacctgaac atgaatttcg aactgtcaga cgaaatgaaa gcactgttca
    19801 aagaaccgaa tgacaaggca ctgaactaat tacaagaacc aggggcggaa attccagccc
    19861 tctcgattgt tacgtagcac ggacagacta tacgcctgat ggtcgttccc catcgggcct
    19921 gaaaaccgca atacgctggg taacaatctt cgagggtagc agttaacgct gctaccctct
    19981 tttttctgga gtagatttat gtctatagac tggaactggg gtattttttt acaacaagcc
    20041 ccgttcggca ataccaccta tcttggttgg gtctggagtg gttttcaggt gacgatcgct
    20101 ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt catttttcgg cattttacgt
    20161 accgttccaa accgttttct ctccggtctg ggtacgttgt atgtcgaact gttccgcaac
    20221 gtgccgttga ttgtgcaatt ctttacctgg tatctggtga tcccggagct gctgccggag
    20281 aaaatcggca tgtggtttaa ggccgagctg gatcccaata ttcagttttt cctttcatcc
    20341 atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg aacaggtacg cgccgctatt
    20401 cagtcgctac cgcgtgggca gaagaatgcc gcgctggcga tgggcctgac gctaccgcag
    20461 acttatcgat acgtactgct gcctaatgct tatcgcgtta tcgtcccgcc gatgacttcc
    20521 gagatgatga acctggtgaa aaactccgcc atcgcctcga ccatcggtct ggtggatatg
    20581 gcggcgcagg cgggtaaatt gctcgactac tcggctcacg cctgggagtc gtttaccgcc
    20641 atcacgcttg cgtatgtgtt gattaacgct tttatcatgc tggtgatgac gctggttgaa
    20701 cgtaaagttc gcctgcccgg caacatgggg ggcaaataat gtacgagttt gactggagtt
    20761 ccattgttcc ttccctgcca tatctgctcg acgggctggt gatcaccctg aaaatcaccg
    20821 tcacggcagt cgtgattggt attttgtggg gtaccatgct ggcggtgatg cgcttgtcca
    20881 gcttcgcgcc tgtttcgtgg tttgccaaag cctacgttaa cgtattccgc tcgattcctt
    20941 tagtcatggt tttgctgtgg ttttacttga ttgtgccggg atttctgcaa aatgtgctgg
    21001 gattatcgcc aaaaaatgat attcgtttaa tttcggcgat ggtggcgttt tcgatgtttg
    21061 aagcggccta ctattcagag attatccgtg ccggtattca aagtatttcc cgtggtcagt
    21121 cgagcgccgc gctggcgttg gggatgactc actggcagtc gatgaaactg attattctgc
    21181 cgcaggcttt ccgcgcgatg gtgccgctgc tgctcactca gggcatcgta ctgttccagg
    21241 atacctcgct ggtgtatgtg ttatctctgg ccgatttctt ccgtaccgcc tcaaccattg
    21301 gtgagcgtga tggtacgcag gtcgagatga tcctgtttgc cggatttgtt tatttcgtta
    21361 ttagccttag cgcgtcgttg ttggtcagct acttgaaaag aaggacagca taatgattac
    21421 cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg ctgaccgact gctcaaccga
    21481 agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct ggttccggca aatcaacgct
    21541 gattaaaacc gtcaacggcc tcgaaccagt gcagcaaggt gaaatcaccg tcgatggtat
    21601 cgtggttaac gacaagaaaa ccgatctggc aaagctgcgc tcccgcgtcg ggatggtatt
    21661 ccagcatttc gagctgttcc ctcatctgtc gattatcgaa aacctgaccc tggcgcaggt
    21721 gaaagtgctt aaacgcgata aagcgcccgc tcgtgaaaaa gcattgaaat tgctggagcg
    21781 cgtgggactt tccgcacatg ccaataaatt tccggcgcag ctttccggcg gtcagcagca
    21841 gcgcgtggca atcgctcgcg cattgtgtat ggctcctatt gcgatgctgt tcgacgaacc
    21901 gacatcggcg ctggatccgg agatgatcaa cgaagtgctg gacgtgatgg tggaactggc
    21961 gaacgaagga atgaccatga tggtggtgac ccacgaaatg ggctttgccc gcaaagtggc
    22021 gaatcgggtg atctttatgg acgagggtaa aattgtcgaa gactcgccga aagacgcttt
    22081 cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc gcgaaaatcc tgcattaatc
    22141 actctggcgc acatacccaa ttgcgcgcca tctcacgctt tatcgtttcg cccctcgtat
    22201 ctgttacatc ctggcgttac ccttgtcgca aagaagcaca caacaaggag caacaatggc
    22261 actgccaatt ctgttagatt gcgacccagg tcatgatgac gctatcgcaa tagttctcgc
    22321 actcgcctca ccagagcttg atgtcaaagc aattacgtct tccgccggaa accagacacc
    22381 agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg cttaatcgca ccgatattcc
    22441 ggtggcaggc ggcgcggtaa aaccgttaat gcgtgagttg attatcgcgg acaatgtgca
    22501 cggcgaaagc ggtctcgacg gcccggcatt accggaaccg acgttcgcac cgcaaaactg
    22561 tacggcggtg gagctgatgg cgaaaacgct gcgcgaaagt gcggaacctg tcacgattgt
    22621 ttctaccgga ccgcaaacta acgttgcctt gctgctcaat agccatccgg aactgcatag
    22681 caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg cttggtaact ggacgcctgc
    22741 ggctgaattt aatatttacg ttgacccgga agcggcagaa attgtcttcc agtcagggat
    22801 cccggtggtg atggccggtc tggatgttac tcataaagcg caaatccacg ttgaagacac
    22861 cgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt gttgccgaac tgctggattt
    22921 cttcctcgaa tatcataaag acgaaaaatg gggctttgtc ggcgcaccgc tgcatgaccc
    22981 atgcaccatc gcctggctgt tgaaacccga gttgtttacc actgttgagc gctgggttgg
    23041 cgtggaaaca caggggaaat atacccaggg tatgacggtt gttgattatt attatctgac
    23101 tggcaataaa ccgaatgcca ctgtaattgt cgatgttgat cgtcagggct ttgttgattt
    23161 actggctgat cgtctgaaat tttacgctta acactcgtca ttgattaaga tcaccacgcg
    23221 ttacctgggt ggtcttaatc tatttatctc tttttctttc aagatataaa tgcaatggat
    23281 aatgcagaac tcgccatagg tatcgatctc ggcactacca atagtttaat tgccgtctgg
    23341 aaagacggtg ccgcgcaatt aattccaaat aagttcggtg aatatttaac accatccata
    23401 attagcatgg atgaaaataa tcatatttta gtcggaaaac cggctgtatc acggcgtact
    23461 tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa tgggcagtaa taccaactgg
    23521 cggttaggca gcgacacatt taacgcgcca gaactgtcct ctttggtatt acgctcatta
    23581 aaagaagatg ccgaagaatt tctgcaacgt ccgattaaag atgtggtgat ctccgttctg
    23641 gcttatttca gcgatgaaca acgcaagcat acccgtttag cagcggactt agccgggtta
    23701 aatgcggtac gcttaattaa tgaacccaca gcagctgcga tggcgtatgg cctgcatacc
    23761 caacaaaata cccgttcgct ggtgtttgat ctcggtggcg gcacgtttga cgttacggtg
    23821 cttgagtacg ccacgccggt gattgaagtt cacgcctccg ctggcgacaa cttcctcggt
    23881 ggcgaagatt ttacccatat gttggtcgat gacgttttaa agcgcgcgga tgtcacaagg
    23941 accacgctta acgagagtga actggcagcc ttgtacgcct gtgtggaagc ggcaaaatgt
    24001 agcaatcaat cgccaatgca cattcgctgg tagtaccagg atgaaacgcg ggaatgcgaa
    24061 ttttacgaga acgaactgga agatttgtgg ttgccgctgc tcaatcgctt gcgagtgccg
    24121 attgaacagg cgttgcgcga tgcgcgtctg aagccgagtc aaatcgacag tctggtgctg
    24181 gttggcggcg cgtcacaaat gccgctggtg cagcgaatcg ccgtgcgtct gtttggcaaa
    24241 ttaccgtatc aaagttacga tccgagcacc attgtcgcgc tgggcgcagc aatccaggcc
    24301 gcctgccgct tacgcagtga agatattgaa gaagtgatcc tcactgatat ttgcccttac
    24361 tcgttgggcg ttgaggttaa ccgccagggc gtttccggca ttttctcgcc gattattgaa
    24421 cgaaacacca ctgtgcccgt gtcgcgtgta gaaacttatt caaccatgca cccggaacag
    24481 gattcaatta cggttaacgt ctatcaggga gaaaaccaca aagttaaaaa caacattctg
    24541 gtggaatcct tcgatgtgcc gttgaagaaa accggggctt atcagtcgat tgatattcgc
    24601 tttagttatg atatcaacgg gttgcttgaa gttgacgtgc ttctggaaga cggcagcgtt
    24661 aagtccagag tgattaacca cagcccggta acattgagcg cgcagcagat tgaagagagt
    24721 cggacgcggt tatccgcatt gaaaatttat ccgcgcgata tgctcatcaa tcgcaccttt
    24781 aaagccaaac tggaagagtt gtgggcgcgg gcgctgggtg acgagcgaga agagattggc
    24841 cgggtgatca ccgattttga tgcggcgctg cagtcaaacg atatggcccg cgtcgatgag
    24901 gtcaggcggc gggcgagcgt ttatttagcc attgagacct cataatcacg gctgccggat
    24961 gcggcgtaaa tgctgcatcc ggcatcggcg caaaaaacac atcgtcaact aaacaacatc
    25021 gcgattttgc cgagagcata acctataacc agcaccgtta aactgatttt gataatgctg
    25081 gtaatgctct gtggtttttc accctcatca tttttttctt catggttttc aggtgttggc
    25141 tcatcgacat cttcatcctc cggctcgagt tttactttcg tcaacagcat ccgggtgata
    25201 atggcctcta atggatgagc atctccgcct tccagccttg tattaagacg ggccagcatc
    25261 tcttgtttcg gaaacgcttc aagtactacc tgttccgcta cccgatacaa atccagtaac
    25321 ggtgaatctt cttctgtctc gctaccgact ctcgatttcc accacgccca tgctttgcgt
    25381 ttgaagaata acgactcaat aaacgattga cctaacggcg ataattttcc gctatgtaaa
    25441 atttccgccc ataacagccg cgtccgggcg ctctcaccag gaacatattt cagcggttga
    25501 ccttgcgcat ctaccatgct ccgcgcttcg ataaccatca ctaataacgc gaaataatcc
    25561 gggcaatgac gcttgctcca ctgcaacaac aaattatcag cttgtgtaga tgggtaactt
    25621 tcccagtacg cgccgagatc agcaagcagg ctttcctcct cgccgcagta caaacgttgt
    25681 gcacactggt aataccgggc atcttcgctt tctggttctt ccgcttgcca ctgtttggct
    25741 acaggaatga gttccaccat acctgtttgc accgagctgt accagcgcaa cagtttgcga
    25801 tgtaactttg catcatcagg aatgaaccac ggtccttcca tcgccaaaaa cgcagtgaca
    25861 tagtcaggat ggtaacggca gcaacgttcc agcgcgaagt aatattcaac agtctggttc
    25921 tgtaacgcga cgggatattc cagaaggcta aggaaatcga aacaatcacc agcctgaatg
    25981 gcctcaagaa aagcctccct ttcttcctca tccattcctt cgctgtcatt aaacgaatgc
    26041 cagttcaaac gctctgccaa caaagaagca caatttaatg acagatatcg cgcttctata
    26101 gcgattgcac acagcggcca gcgtaattga tcgacatcct ccatgttcca ggtatttaat
    26161 tgctgaataa atttctgcca ggcggagggc tgaaaacgat cactttctga atccagtaat
    26221 gtcctgaatg cacgtagtat ttcatgttca gcggtggcat ctttttcttc atcatctgct
    26281 tcctcgacag gatttaccgc cagtcgcagc gcctcttcat aggcctggcg taatgcttta
    26341 aacccctgcg gatcggtttc gggatgacac aacggtaagc gagcaagata agcctggcgg
    26401 ataatgtcta tttgcgtcgt gctttcaatt tcgaggattt gccaacatgt tttcataggt
    26461 cgctcacatc cttaagcgtt accgggcatt caggctggac agttaatggc tgccagggaa
    26521 gcgtggcata gaaacggtcg cttcccgctg ccagctcttc gaacatccga ctcccggcgc
    26581 tatcgtattt gtacagggtt agcgcctcgc gtagcgaagc gttgtcttcg cactgagaag
    26641 attgccagta gagacgccct agtgaatagg cagaaaaata gtgcatccag ctgtgatagt
    26701 aatggtgagc tcgtgcatag acgcgcgatt gcagccataa actctcttct tccgtcagcc
    26761 agttgttgag cacaccggcc cgtgacagaa atcccattcg aacataatcc caggctttga
    26821 ttcccccttc tccacagcac atagcggttt gggcaacgaa ttgggtataa atcaaccctc
    26881 tctccgagcc accagcaatt aatgctttcc actcttcagg ggaagagcgt tgccatgtca
    26941 gatataagcc agctaaatca ttggcatgac catcatcagt catctgataa atagtctgaa
    27001 ttaaatcatc acgattattt attccccagg atgatgagag atcgaattct tttatcccgg
    27061 tatacatttt tggcgagtca tgcgcgggtt tgcaactctg attaagtatt tccatgggcg
    27121 cagaaagcgc aaaaagaaga tattgttctt ccttattcat tttttaataa tcccttaagt
    27181 gcattaactg actacttttt tattattgat gagtataaac ttgacgcttt caaaataaaa
    27241 agaaaatcga agcattcaca catgaataaa aaattaatgt atatattcgc aatttttata
    27301 gttgcagcaa ttacctgtat tagccaaccc aagaaaacga tgttgcgtga taaagccatg
    27361 gtgaattatg cctttgatta tttaagatca ccaggcagtc taccgttaac cacggcagcc
    27421 acggagcttt ccgcgattca tggtcactca acatcgcaat atcgccttgg cgaattttat
    27481 cttcatggta gcgacggtaa accactggat tatacacagg cgagatactg gtatgagcaa
    27541 tcagcagaac aggaaaatcc gcgcgcacaa agcaaactgg ggtggatcta cctgaaaggc
    27601 ctgggagtca aacccgatac ccgtaaagca attctctggt ataaggaagc agctgaacaa
    27661 gggtatgctc atgctcaata tactttgggt ttgatctaca gaaatggcac tggtattaat
    27721 gttaaccatt atgaatctca aaaatggtta aaactggccg ccaaacaaca ttacaaaaat
    27781 gcggaaagat tacttgccgg acttcccgca cattaacgtc gactgtcacc cgaagagatg
    27841 gagtattttc cctatcaaac cggcaaagat aaaaatgtag aaaataattt tgatgacgcc
    27901 cagacagccc agcccgccat catcaacggg cattggctgc ggttgcggct ccggctcgac
    27961 ggtttcctga ccggtaaaat tcgccgcctg aattaataat cgacgaataa ttaatgcttc
    28021 cagtggacaa cgatcttctg catcaaggcg gctcacaatc ggcattaatg attcttctgt
    28081 cggttggctg gcctgcacta attgtttggc aactaaatat aatggccata agggatgttc
    28141 tgtatcacgc gtgagtgaga tccgcggctt acgtcctttg tgcaaaacca tctcgaccag
    28201 tgaacgcccc agcgcagaga gtttttcatc gctgagtatt tcgtataaat gaaaacgcgt
    28261 acgggcgctg tcgccgggga cataaagtag cggttttccc tgatcgttaa ccagatcacg
    28321 cgcttcaatc atcatcacca ctaatgggta ataatagacc cgatgctgac ggcaccattg
    28381 caacattaaa gcatccgcct gggtggaggg atactcgcgc cagtagtaac aaagttctgc
    28441 cagcaggctt tcgccttctc cacaatagac gcgttgtgcg tattcataat aacgcgcatc
    28501 ttcattatca ggttcagctt cccgccacga ccgggcgtaa tccaggagtt cggggattcc
    28561 caggcggctt gcgctatacc agcggagtaa atttctgtgc agttttgcat catcggggat
    28621 aatcactgcg cgatgttgcg ctaaatacaa tgtcagccat tctggatgcg atgaccaaat
    28681 ccgagcgaac atttggtaaa aatcgatggt gtcattttgc acggcaactg gcagatgcag
    28741 aatggtctgg aagttaaaaa cattcccctt cgcaatggca tataaaaagg attccagttc
    28801 ttcttcgtct atttccccga cgttttcctc ctgcaaccac cgcaatcttt ctgctaataa
    28861 caccacgcac tcgaaggata aatgggcagt gttcatggcg attgtgcaca gcgaccagcg
    28921 taattcatca atctcttcca tcgagcaata atttaattgc tgaatgaatc gctgccaggc
    28981 ggagggcaga aaacgttcgc tatcagaggc aagtaacgcg cgaaaggcga gcagaatttc
    29041 atgttctgct acctcatatt cttctggttg ccaaacagat ttagcaggcg actgcgcaat
    29101 ccgtagcgcc tcctcatacg cctgacgaag ttgtttaaaa ccctgcggat cggtttctgg
    29161 atgaaaggac ggtaacagcg ccagataagc gcggcggata atatcgacgt cagtcgtttc
    29221 ctctatatcg aggatcttcc agcaattttt cataagtcgc tcatatcctt aagcgtttcc
    29281 gggcatgtgg ggtagtcagc cagtggttgc caggacaagg tagggtaata cgcatcatca
    29341 ctggcaaata attgataaaa catatcattg ccggaatcgt cgtattcttt acgcgctaag
    29401 aattcgcgta ataaaggcag atgatcatct tcgggagact gccaatattg ccgaccaaag
    29461 gtataaccgg caaagtattg ccgccagtta ctataataac gtagagcccg tagatgaata
    29521 cgcgactgga tccatagact ttcttcttca ctcaaccagt tattaagtac gcccattcga
    29581 cttaaaaaac ccatccggac ataatcccat gctttaattc ctccctcacc acagcattcg
    29641 gctgtgctgg cgacaaattg cgcataagcc tgaccttgct catttaattc agcaagatag
    29701 tcacgccatt cacatggaga ataacgaaac cagcggtgat aaaaccctgc caaccgagcg
    29761 gcatgaccgt tctcgatcat tcggtgaatt atgcgcagca ggtcatcacg gtcatcaatt
    29821 ccccatgagg aagaaaggtc aaaaaagcat gtacctaaat acattttggg ggatgaatgc
    29881 gaaggtaaac agcattcatt gcgtatttcc atcggagtgg aaagtgcaaa caatattttt
    29941 tgcgacccca tgtccatata atttatcctt aaacattcat ttaatgaatg taaactacat
    30001 ttcgaggctc agttatttat acttttctga cgcaacataa tacgcataaa ataaggctta
    30061 cgcaaaaatg gaacacataa aaatataata tattttatct acaaccataa ggaaatgata
    30121 atgattttta cgtcaagttg ctgcgataat ttatcaatag atgagattat cgaacgtgct
    30181 gaaaaaggcg attgcgaggc tcaatatatt gttgggtttt attataatcg cgatagcgca
    30241 attgactctc cggacgacga aaaggccttt tactggctga agctggccgc tgagcaaggt
    30301 cattgtgaag cacagtattc cttagggcaa aagtataccg aggataaaag ccgtcataaa
    30361 gataatgagc aagccatctt ctggctgaaa aaagctgccc tacaaggcca tactttcgct
    30421 tccaacgccc ttggctggat actggatcgt ggagaagacc ccaactataa agaagcggtt
    30481 acctggtatc agatagccgc ggagagcgga atgtcttatg cgcaaaataa tcttggatgg
    30541 atgtacagaa atggcaacgg agtcgcacaa gactatacgc tggcattttt ctggtacaaa
    30601 caagctgcat tacaaggcca tagttacgca caagacaatc tggccgatct ttataaagac
    30661 ggagaaggcg ttgctcaaaa caagacactc gccgcattct ggtatttgaa aagcgcacag
    30721 cagggtaatc ggcacgccca gtttcaaatt gcatgggatt ataacgctgg cgaaggggtg
    30781 gaccaggact ataagcaagc gatgtactgg tatctgaagg ctgccgctca ggaaagcgtc
    30841 ggcgcttacg tcaacatcgg ttatatgtat aaacacggac aaggcgttga gaaagattat
    30901 caggctgcct ttgaatggtt tacgaaagcc gctgaatgca atgacgccac tgcctggtat
    30961 aacctggcca ttatgtatca ctacggagaa ggaagacctg tcgatctccg acaggctctc
    31021 gacctgtatc gtaaagttca gtcatccgga accagagatg tcagtcagga aattcgtgag
    31081 actgaagctt tactgtagaa ataaaaacaa gggccgggga atttcattct gcccatattc
    31141 tctttattgc tgtgcgaaat cagactatta cggctcaaac gggcgtctct ggaactggtc
    31201 ataaccacat ttcgggcata gcgtcagcac ttccggtgtg tagatcggga gatggaagtg
    31261 acatttctcg cagaccagat ttcccagccc gaccacttct ccgctgtgat aaaccccatg
    31321 atgattgagg tcctggaaaa tttcgcgcca ttcaagctgc gttttatcgg tgatgtctgc
    31381 cagctcctgc cacaagcttt ctttaatcac ccgcataaag acgctgtcag attcttcttt
    31441 caggctctct tcatagctca tggcgaactc ttccaggtca cgtctgacag ctcgcgtcag
    31501 ctcatcgatc tcggttcgcg ttaactcccc tgtttttatt acgcgctcgc gcgcctgttc
    31561 caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt tcgttcagtg acgcgaccag
    31621 ttcacggtaa tattgagcaa ccttgttcat cgtttcatct cccgggtcgt taactgacta
    31681 taataataga cgttctttgt tccttgcttt gctaatacgc ccacaaactc tgtaaattaa
    31741 tgtacgaata atccgggcat catttcaacg tcgagtgcgc gaaaggctgt tttgacgcgg
    31801 acgtttgggc tatgctatgc ggatctgaaa aaccacctca acgctacatt tgtagccgta
    31861 ttgaaaacag gaccactggc tgccatgcaa gagcaatacc gcccggaaga gatagaatcc
    31921 aaagtacagc ttcattggga tgagaagcgc acatttgaag taaccgaaga cgagagcaaa
    31981 gagaagtatt actgcctgtc tatgcttccc tatccttctg gtcgactaca catgggccac
    32041 gtacgtaact acaccatcgg tgacgtgatc gcccgttacc agcgcatgct gggcaaaaac
    32101 gttctgcagc cgatcggctg ggacgcgttt ggtctgcctg cggaaggcgc ggcggtgaaa
    32161 aacaacaccg ctccggcacc gtggacgtac gacaacatcg cgtatatgaa aaaccagctc
    32221 aaaatgctgg gctttggtta tgactggagc cgcgagctgg caacctgtac gccggaatac
    32281 taccgttggg aacagaaatt cttcaccgag ctgtataaaa aaggcctggt atataaaaag
    32341 acttctgcgg tcaactggtg cccgaacgac cagaccgtac tggcgaacga acaagttatc
    32401 gacggctgct gctggcgctg cgataccaaa gttgaacgta aagagatccc gcagtggttt
    32461 atcaaaatca ctgcttacgc tgacgagctg ctcaacgatc tggataaact ggatcactgg
    32521 ccagacaccg ttaaaaccat gcagcgtaac tggatcggtc gttccgaagg cgtggagatc
    32581 accttcaacg ttaacgacta tgacaacacg ctgaccgttt acactacccg cccggacacc
    32641 tttatgggtt gtacctacct ggcggtagct gcgggtcatc cgctggcgca gaaagcggcg
    32701 gaaaataatc ctgaactggc ggcctttatt gacgaatgcc gtaacaccaa agttgccgaa
    32761 gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg gctttaaagc tgttcaccca
    32821 ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg tattgatgga gtacggcacg
    32881 ggcgcagtta tggcggttcc gggtcacgac cagcgcgact acgagtttgc ctctaaatac
    32941 ggcctgaaca tcaaaccggt tatcctggcg gctgacggct ctgagccaga tctttctcag
    33001 caagccctga ctgaaaaagg cgtgctgttc aactctggcg agttcaacgg tcttgaccat
    33061 gaagcggcct tcaacgccat cgccgataaa ctgactgcga tgggcgttgg cgagcgtaaa
    33121 gtgaactacc gcctgcgcga ctggggtgtt tctcgccagc gttactgggg cgcgccgatt
    33181 ccgatggtga cgctggaaga cggtaccgta atgccaaccc cggacgacca gctgccggtg
    33241 atcctgccgg aagatgtggt gatggacggc attaccagcc cgattaaagc agatccggag
    33301 tgggcaaaaa ctaccgttaa cggtatgcca gcgctgcgtg aaaccgacac tttcgacacc
    33361 tttatggagt cctcctggta ctatgcgcgc tacacttgcc cagagtacaa agaaggtatg
    33421 ctggattccg aagcggctaa ctactggctg ccggtggata tctacattgg tggtatcgaa
    33481 cacgccatta tgcacctgct ctacttccgc ttcttccaca aactgatgcg tgatgcaggc
    33541 atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc agggtatggt gctggcagat
    33601 gccttctact atgttggcga aaacggcgaa cgtaactggg tttccccggt tgatgctatc
    33661 gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag atgcggcagg ccatgaactg
    33721 gtttataccg gcatgagcaa aatgtccaag tcgaagaaca acggtatcga cccgcaggtg
    33781 atggttgaac gttacggcgc ggacaccgtt cgtctgttta tgatgtttgc ttctccggct
    33841 gatatgactc tcgaatggca ggaatccggt gtggaagggg ctaaccgctt cctgaaacgt
    33901 gtctggaaac tggtgtacga gcacacagca aaaggtgatg ttgcggcact gaacgttgat
    33961 gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc ataaaactat cgctaaagtg
    34021 accgatgata tcggccgtcg tcagaccttc aacaccgcaa ttgcggcgat tatggagctg
    34081 atgaacaaac tggcgaaagc accgaccgat ggcgagcagg atcgcgctct gatgcaggaa
    34141 gcactgcagg ccgttgtccg tatgctcaac ccgttcaccc cgcacatctg cttcacgctg
    34201 tggcaggaac tcaaaggcga aggcgatatc gacaacgcgc cgtggccggt tgctgacgaa
    34261 aaagcgatgg tggaagactc cacgctggtc gtggtgcagg ttaacggtaa agtccgtgcc
    34321 aaaatcaccg tcccggtgga cgcaacggaa gaacaggttc gcgaacgtgc tggccaggaa
    34381 catctggtag caaaatatct tgatggcgtt actgtacgta aagtgattta cgtaccaggt
    34441 aaactcctca atctggtcgt tggctaagcg cgggaggaag cgtgcgatat ctggcaacac
    34501 tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg ctggcatctg cgtgacacca
    34561 cgcaggttcc ttccactatg aaggtcatga tcctggactc aggcgatccg aacgggccat
    34621 taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt cgagttgctt gataaagaaa
    34681 ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt gagcatcgcg aaagataccg
    34741 catcggtatt ccgtaacggt caaacagcag agtatcagat gatcatgacg gtgaatgcga
    34801 ccgtgttgat ccccggccgt gatatctacc cgattagcgc caaagtcttc cgttcattct
    34861 tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca agacatgatc gtaaaagaga
    34921 tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc aagcatccgt gctgcggata
    34981 ttcgttccga cgaagaacag acgtcgacca caacggatac tccggcaacg cctgcacgcg
    35041 tctccaccat gctgggtaac tgatgattcg gttgtacccg gaacaactcc gcgcgcagct
    35101 caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac gatcctctgt tattgcagga
    35161 aagccaggac gctgttcgcc aggtagctgc ggcacaagga ttcgaagaac accacacttt
    35221 ttccattgat cccaacactg actggaatgc gatcttttcg ttatgccagg ctatgagtct
    35281 gtttgccagt cgacaaacgc tattgctgtt gttaccagaa aacggaccga atgcggcgat
    35341 caatgaacaa cttctcacac tcaccggact tctgcatgac gacctgctat tgatcgtccg
    35401 cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg tttactgctc ttgcgaatcg
    35461 cagcgtgcag gtgacctgtc agacaccgga gcaggctcag cttccccgct gggttgctgt
    35521 gcgcgcaaaa cagctcaact tagaactgga tgacgcggcg aatcaggtgc tctgctactg
    35581 ttatgaaggt aacctgctgg cgctggctca ggcactggag cgtttatcgc tgctctggcc
    35641 agacggcaaa ctgacattac cgcgcgttga acaggcggtg aatgatgccg cgcatttcac
    35701 cccttttcat tgggttgatg ctttgttgat gggaaaaagt aagcgcgcgt tgcatattct
    35761 tcagcaactg cgtctggaag gcagcgagcc ggttattttg ttgcgcacat tacaacgtga
    35821 actgttgtta ctggttaacc tgaaacgcca gtctgcccat acgccactgc gtgcgttgtt
    35881 tgataagcat cgggtatggc agaaccgccg gggcatgatg ggcgaggcgt taaatcgctt
    35941 aagtcagccg cagttacgtc aggccgtgca actcctgaca cgaacggaac tcaccctcaa
    36001 acaagattac ggtcagtcag tgtgggcaga gctggaaggg ttatctcttc tgttgtgcca
    36061 taaacccctg gcggacgtat ttatcgacgg ttgatatgaa atctttacag gctctgtttg
    36121 gcggcacctt tgatccggtg cactatggtc atctaaaacc cgtggaaacg ctggcgaatt
    36181 tgattggtct gacgcgggtc acaatcatcc ctaataatgt tcctccgcat cgtccccagc
    36241 cggaagcgaa cagcgtgcag cgtaaacaca tgcttgaact ggcgattgcc gacaagccat
    36301 tatttactct tgatgaacgc gagctaaagc gcaatgcccc ctcttacact gcgcaaacac
    36361 tgaaagagtg gcggcaggaa caaggaccgg acgtgccgct ggcgtttatt attggtcagg
    36421 attcattgct gacctttccg acctggtacg aatacgaaac gatactcgac aatgcacatt
    36481 tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc gcaaccgcaa taccagcaat
    36541 ggctggaaga tcatttgaca cataacccgg aagatcttca ccttcagcct gccggtaaaa
    36601 tttatctggc tgaaacgccg tggtttaaca tctcggcgac catcatccgc gaacgtttgc
    36661 aaaacggtga atcgtgtgag gatttattgc cggaaccggt attgacttac attaaccaac
    36721 aaggcttgta tcgctgatac ctgtcgttac ggagggcatg atgcgactgt ggttaattcg
    36781 tcatggcgaa acacaagcga atgtcgatgg tctgtacagc ggtcatgcgc ccacacccct
    36841 gaccgcacgc ggtattgagc aagcgcaaaa tctgcataca ctgctagatg atgtttcctt
    36901 tgacttggtt ttatgcagtg aactggaacg ggcacagcat accgcgcgac tggttctcag
    36961 tgaccgccag ctccccgttc atatcatccc tgaactcaac gaaatgtttt ttggcgactg
    37021 ggagatgcga catcatcgcg acctcatgca agaagatgcc gaaaactata gcgcgtggtg
    37081 caatgactgg cagcatgcta tccccacgaa cggtgaagga tttcaggcat tttcgcaacg
    37141 tgtggaacgc tttatcgcaa ggcttagtga atatcagcac tatcagaata ttttaatcgt
    37201 cagccatcag ggcgtactga gtctgttaat tgctcgttta attggcatgc ctgccgagtc
    37261 tatgtggcat tttcgcgttg accaggggtg ttggagcgcc attgatatca accaaaaatt
    37321 cgcgacgcta cgcgtcctca atagccgtgc catcggggtc gagaacgcat gacttttctg
    37381 tttttttacg ggtaagccgc aacgaccatt gacagccccg ggcaggctga tattctccgc
    37441 agccagactt tttccgccag acacgacttt gtagaaattg ttttacaaaa atggcgatgc
    37501 aatctgcggc gcggggtggg atgatagccc actttcgaaa gccgattcgg cgacaattgt
    37561 cccgaaatcg cctctggttc aggtatactg acagaccatt tttatctatt tgattcaccc
    37621 agggggaaaa cttgcagggt aaagcactcc aggattttgt tatcgacaaa attgatgacc
    37681 tcaaaggtca ggacatcatc gccttagacg ttcaaggcaa atccagcatc accgactgca
    37741 tgatcatctg tacgggtacg tccagccgtc atgttatgtc cattgctgac cacgttgtgc
    37801 aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga aggtgaaaac agcgccgact
    37861 ggattgtcgt ggatttgggc gatgtgattg tccatgtcat gcaggaagag agccgtcgcc
    37921 tgtatgaact ggaaaaactc tggagttaat gcgtgaagct gcaacttgtc gccgtgggaa
    37981 cgaaaatgcc ggactgggta caaaccggtt ttaccgagta cctgcgtcgt tttccgaaag
    38041 atatgccctt cgagctgatt gaaattccgg ccggcaaacg cggcaagaat gcggacatca
    38101 agcgcatact cgacaaagag ggtgagcaga tgttggcggc cgcaggcaaa aaccgcattg
    38161 tcaccctcga tattccaggc aagccctggg atacgccgca gttagccgct gagctggaac
    38221 gctggaagct ggatggtcgc gacgtaagtc tactgattgg cgggcctgaa gggttgtcgc
    38281 ctgcctgtaa agcggcggct gagcagagct ggtcgctgtc ggcgcttacc ctcccccatc
    38341 cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc gtggagcatc accaccaacc
    38401 atccttatca ccgtgagtga taagggagct ttgagtagaa aacgcagcgg atgaaactac
    38461 agaactcttt tcgcgactat acggcagagt ccgcgctgtt tgtgcgccgg gcgctggtcg
    38521 cctttttggg gattttgctg ctgaccggcg tgcttatcgc caacctgtat aatctgcaaa
    38581 ttgttcgctt taccgactac cagacccgct ctaatgaaaa ccgcattaag ctggtgccta
    38641 tcgcgcccag ccgcggcatt atctacgacc gtaacggtat ccctctggcc ctcaaccgca
    38701 ctatctacca gatagaaatg atgccggaga aagtcgataa cgtgcagcaa acgctggacg
    38761 ctttgcgcag cgtggtagat ctgaccgatg acgatattgc tgcattccga aaagagcgcg
    38821 cacgttcaca ccgtttcacc tctattccgg tgaaaactaa cctaaccgaa gtacaagtag
    38881 ctcgctttgc cgtcaatcag taccgttttc cgggtgtcga agttaaaggc tataaacgtc
    38941 gttactatcc ttacggttcg gcgttgaccc acgtcatcgg ctatgtgtcg aaaatcaacg
    39001 ataaagacgt cgaacgcctg aataatgacg gcaaactggc caactatgcg gcaacgcatg
    39061 atattggtaa gctgggcatt gagcgttact atgaagacgt gctgcacggt cagaccggtt
    39121 atgaagaggt tgaagttaac aaccgtgggc gtgttatccg ccagttaaaa gaagtaccac
    39181 cgcaagccgg acacgatatt tacctgacgc tggatctcaa actccagcaa tatattgaaa
    39241 cgctgctggc gggtagccgc gcagctgtgg ttgttaccga accgcgtaca ggtggggtgc
    39301 tggcgctggt ttccacgcct agttatgacc caaacctgtt tgttgacggt atctccagca
    39361 aagattattc cgccttgctg aacgacccga atacaccgct ggtgaaccgc gccacacagg
    39421 gggtttatcc tcccgcgtct acagttaaac cctatgtggc ggtttcggca ttaagcgccg
    39481 gggtgatcac gcgcaatacg acgctgtttg acccaggctg gtggcaacta ccagggtcgg
    39541 aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg tctgaatgtc acaagatcgc
    39601 tggaagaatc tgcggatacc ttcttctatc aggtggccta cgatatgggg atcgatcgcc
    39661 tctccgaatg gatgggtaaa ttcggttatg gtcattacac cggtatcgac ctggcggaag
    39721 aacgttccgg caacatgcct acccgcgaat ggaaacagaa acgctttaaa aaaccgtggt
    39781 atcagggtga caccattccg gttggtatcg gccagggtta ctggacagcg accccaatcc
    39841 agatgagtaa ggcactgatg atcctgatta atgacggtat cgtgaaggtt ccgcatttgc
    39901 tgatgagcac cgccgaagac ggcaaacagg tgccatgggt acaaccgcat gaaccgcccg
    39961 tcggcgatat tcattccggt tactgggagc tggcgaaaga cggtatgtac ggtgttgcta
    40021 accgccctaa tggtacggcg aataaatact ttgccagcgc accgtacaaa attgcggcga
    40081 aatctggtac cgctcaggtc ttcggtctga aagcgaacga aacctataat gcgcacaaaa
    40141 ttgccgagcg tttacgtgac cacaaactga tgaccgcctt tgcgccatac aacaatccgc
    40201 aagtggctgt cgccatgatt ctggagaacg gcggcgcggg tccggcggtt ggtacgctga
    40261 tgcgccagat cctcgaccac attatgctgg gtgataacaa caccgatctg cctgcggaaa
    40321 atccagcggt tgccgcagcg gaggaccatt aatcatgacg gataatccga ataaaaaaac
    40381 attctgggat aaagtccatc tcgatcccac aatgctgctg attttactgg cattgctggt
    40441 ttacagcgcc ctggttatct ggagcgccag cggtcaggat attggcatga tggagcgtaa
    40501 aatcggccaa atcgcgatgg gtctggtcat catggtggtg atggcgcaaa ttcctccacg
    40561 cgtttatgaa ggctgggccc cctatctcta tatcatctgt attattttgc tggtggcggt
    40621 agatgctttc ggagccatct ctaaaggcgc tcaacgctgg ctggacctcg gtattgttcg
    40681 ttttcagccg tcggaaattg ccaaaatagc cgtaccactg atggttgcgc gctttatcaa
    40741 ccgcgacgtt tgcccgccat cgttgaagaa tactggcatc gcgctggtgc tgatatttat
    40801 gcccacgctg ctggtggctg cacagcctga cctgggaaca tcaatcctcg ttgcgctttc
    40861 cggtctgttt gtactgttcc tctctggcct tagctggcgt ctgattggcg tcgcagtagt
    40921 gctggtagcg gcgttcattc cgattctgtg gttcttcctg atgcatgatt accagcgcca
    40981 gcgcgtaatg atgctcctgg acccggaatc agacccactc ggcgcgggct atcacattat
    41041 tcagtctaaa attgctattg gctccggcgg attacgcggc aaaggctggc tgcacggcac
    41101 tcagtcacag cttgaatttc tccccgaacg ccatactgac tttatcttcg cggtactggc
    41161 ggaagagctg ggattagtgg gcattctgat tctgctcgct ctctacattc tgctgatcat
    41221 gcgcgggctg tggatagccg ccagagcgca aaccaccttt ggtcgcgtca tggctggtgg
    41281 cttaatgctg atattattcg tttatgtctt cgtaaatatt ggtatggtaa gcggtattct
    41341 gccggttgta ggggttccgc tcccactggt cagttatgga ggatcggcgc taattgtgct
    41401 gatggctggg ttcgggattg taatgtcaat ccacacccac aggaaaatgt tgtcgaaaag
    41461 cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat ctgcatcgcg gcaggaatgc
    41521 tcgcggcatg tacaagcgat gatggtcagc aacagacggt aagtgtaccg cagcctgcgg
    41581 tatgtaacgg ccccatagtt gaaattagcg gggcggaccc gcgtttcgaa ccactgaacg
    41641 cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta caaaatcgtg caggatccgt
    41701 ctcgatttag ccaggcggga ctggcggcaa tctatgatgc cgaaccaggc agtaacctga
    41761 cggcctctgg cgaagctttc gatccgacaa agctgacggc ggcccatcca acgcttccga
    41821 tccccagcta cgccagaatc actaacctgg ctaacgggcg aatgatcgtg gtgcgcatta
    41881 atgatcgcgg cccttacggc aacgaccgcg ttatttcgct ttctcgcgcg gcagctgacc
    41941 gtcttaacac gtcaaacaac accaaagttc gtatcgatcc gattattgtc gcccaggatg
    42001 gttcgctttc tggtcctggt atggcttgta ccacagtcgc caaacagact tacgccctgc
    42061 ctgcacctcc cgatttaagc ggtggcgcgg gaacaagttc agtgtctggc ccgcagggtg
    42121 acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga tccgaccggc gcgccggtaa
    42181 ccagtagcgg tttcctcggc gctccaacga ccttagcgcc tggtgtactg gaaggcagcg
    42241 aaccgacgcc tgcgccacag cccgttgtta cagcttcgtc gacaacgcct gcaacctcgc
    42301 ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag cggcaacttt atggtgcaag
    42361 tcggggccgt aagcgatcag gctcgtgcgc aacagtacca acagcaactg ggacagaagt
    42421 tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg gcggatccag cttggcccat
    42481 tcgccagcaa agccgaagcc agtaccttgc agcaacgttt gcaaaccgaa gcccaattac
    42541 agtcatttat tactaccgcg cagtagcgta aagcaggcat ctgatgtgtc aatttctgta
    42601 aatgacgtta acaaagtcac gcggaaagtc agatgcctgc cggtagtgca tttgctatag
    42661 tagggcactt ttttaattcc atcacggatg tcgtagttca gaccatgaat accatttttt
    42721 ccgctcgtat catgaagcgc ctggcgctca ccacggctct ttgcacagcc tttatctctg
    42781 ctgcacatgc cgatgacctg aatatcaaaa ctatgatccc gggtgtaccg cagatcgatg
    42841 cggagtccta catcctgatt gactataact ccggcaaagt gctcgccgaa cagaacgcag
    42901 atgtccgccg cgatcctgcc agcctgacca aaatgatgac cagttacgtt atcggccagg
    42961 caatgaaagc cggtaaattt aaagaaactg atttagtcac tatcggcaac gacgcatggg
    43021 ccaccggtaa cccggtgttt aaaggttctt cgctgatgtt cctcaaaccg ggcatgcagg
    43081 ttccggtttc tcagctgatc cgcggtatta acctgcaatc gggtaacgat gcttgtgtcg
    43141 ccatggccga ttttgccgct ggtagccagg acgcttttgt tggcttgatg aacagctacg
    43201 ttaacgcact gggcctgaaa aatacccact tccagacggt acatggtctg gatgctgatg
    43261 gtcagtacag ctccgcgcga gatatggcgc tgatcggcca ggcattgatc cgtgacgtac
    43321 cgaatgaata ctcgatctat aaagaaaaag aatttacgtt taacggtatt cgccagctga
    43381 accgtaacgg cctgttatgg gataacagcc tgaatgtcga cggcatcaaa accggacaca
    43441 ctgacaaagc aggttacaat cttgttgctt ctgcgactga aggccagatg cgcttgattt
    43501 ctgcggtgat gggtggacgt acttttaaag gccgtgaagc cgaaagtaaa aaactgctaa
    43561 cctggggctt ccgtttcttc gaaaccgtta acccactgaa agtaggtaaa gagttcgcct
    43621 ctgaaccggt ttggtttagt gattctgatc gcgcttcgtt aggggttgat aaagacgtgt
    43681 acctgaccat tccgcgtggt cgcatgaaag atctgaaagc cagctatgtg ctgaacagca
    43741 gtgaattgca tgcgccgctg caaaagaatc aggtcgtcgg aactatcaac ttccagcttg
    43801 atggcaaaac gatcgagcaa cgcccgctgg ttgtgttgca agaaatcccg gaaggtaact
    43861 tcttcggcaa aatcattgat tacattaaat taatgttcca tcactggttt ggttaaaaat
    43921 tgaacacttg aaagtgtaat ttccgtcccc atatactaag catcagtaaa aaaactcccg
    43981 ccttctggcg ggagttgcta tttaattacg ttacgccgga gctgacatga aaaccaaact
    44041 taacgaactg cttgaattcc ctactccttt tacttacaaa gttatggggc aggcgttacc
    44101 tgagctggtt gatcaggtgg ttgaagtggt acagcgccat gcgccaggtg actacacccc
    44161 aacggtaaaa ccaagcagca aaggcaacta ccactcggta tctatcacta tcaacgccac
    44221 tcatatcgag caggttgaaa cactgtatga agaactgggc aaaatcgata ttgtccgcat
    44281 ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg ggtaactccc ctctcctcgc
    44341 tgtgatatac ttttccccca cttttactca ttctccacgg agatgccgtt ttgtatcagg
    44401 ataaaattct tgtccgccag ctcggtcttc agccttacga gccaatctcc cgggctatgc
    44461 atgaattcac cgatacccgc gataatagta cccttgatga aatctggctg gtcgagcact
    44521 atccggtatt cacccaaggt caggcaggaa aagcggagca cattttaatg ccgggtgata
    44581 ttccggtgat ccagagcgat cgcggtgggc aggtgactta tcacgggccg ggacaacagg
    44641 tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg tgtgcgtgaa ctggtgacct
    44701 tgcttgagca aacagtggtg aataccctgg ctgaactggg tatagaagcg catcctcggg
    44761 ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg ctcactgggt ttacgaattc
    44821 gacgcggttg ttcattccac ggtctggcat taaacgtcaa tatggatctt tcaccatttt
    44881 tacgtattaa tccttgtggg tatgccggaa tggaaatggc taaaatatca caatggaaac
    44941 ccgaagcgac gactaataat attgctccac gtttactgga aaatatttta gcgctactaa
    45001 acaatccgga cttcgaatat attaccgctt aattccatac atcaatggcc caatttacac
    45061 tgggtcatta ctcataatac ctgctaatgc tttacatctc cttcgcgttt gtatattttc
    45121 tttatcactt gcagaaatgc ggtgccacgc cttgctccgt aactcacccc attgattaac
    45181 ctattgaaat agttgattat ggtgagcaaa agcgttccgt tccaattaga cataagatta
    45241 ttgtaatttt ttaaatatta tttttccatg aataaaattg gagtaagtgc gtggatagta
    45301 ataatcaaat tgaaccctgt ttaagcagaa agtcgtcaga aggtaaacca caaatattta
    45361 caaccttacg aaatattgat cttaaccttc tgactatttt tgaagctgta tatgtacata
    45421 aagggatcgt taatgcagcg aaagtgctta atctgacccc ctcggcaatc agtcagtcta
    45481 ttcagaaact gcgcgttata ttccctgacc cattgtttat tcgcaaaggc cagggtgtca
    45541 ctcctaccgc atttgcgatg catctacatg agtatatcag tcagggcctt gagtccattc
    45601 ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg aacgataact attgcgacca
    45661 ctccctcggt cggagccctg gtccttcctg tcatctatcg ggcgattaaa actcactatc
    45721 cgcagctttt actgcgcaac ccgcccatca gcgacgcgga aaaccaactc agtcagtttc
    45781 aaaccgatct catcatcgat aacatgtttt gcaccaatcg tacggtgcaa catcatgttc
    45841 tgttcaccga caatatggtg ttaatttgcc gcaagggaaa tccactactc tctttagaag
    45901 atgacagaga gactatcgac aacgctgcgc atgtacttct gttaccggaa gggcaaaatt
    45961 tcagcggtct gcggcagaga gttcaagaga tgtttccgga ccggcaaatc aatttcacca
    46021 gctacaacat tttgacaatc gctgcactgg ttgccaacag tgacatgtta gcgattattc
    46081 ccagccgttt ttataacctg tttagtcgtt gctggccgct ggaaaaactg ccttttccgt
    46141 ccttaaatga ggagcaaata gatttctcta tccactacaa caaattcatc ctgcgtgatc
    46201 ccatcctgca cggagtaata gatgttatcc gtaatgcatt ttgaaaaagt attgttacag
    46261 ccagatcaca gcgcagaacg ataaaatggc acaatccaac aacaatttta cattttcgcg
    46321 accgctttgg ctgctttcag gtccgtttca atgatatact gccagtcgtt aattcaaaaa
    46381 tagttgataa ttacaacaat ctattgaatt gaaacgcttt ccttcgtaat tcgcaactgg
    46441 aacacgcacg ctatgagtaa acccattgtg atggaacgcg gtgttaaata ccgcgatgcc
    46501 gataagatgg cccttatccc ggttaaaaac gtggcaacag agcgcgaagc cctgctgcgc
    46561 aagccggaat ggatgaaaat caagcttcca gcggactcta cacgtatcca gggcatcaaa
    46621 gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg aagcctcctg ccctaacctg
    46681 gcggaatgct tcaaccacgg cacagcaacg tttatgatcc tcggcgctat ttgtacccgc
    46741 cgttgtccgt tctgtgatgt tgcccacggt cgcccggtag ctcctgatgc caatgaacca
    46801 gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt atgtggttat cacctccgtt
    46861 gaccgtgatg acctgcgcga tggcggtgcc caacactttg cggattgtat tactgccatt
    46921 cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc cggatttccg cggtcgaatg
    46981 gatcgtgctc tggatattct gactgcaacg cccccagatg tgttcaacca taacctggaa
    47041 aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag attacaactg gtcgctgaag
    47101 ctgctggaac gctttaaaga agcgcatccg gaaatcccga ccaagtctgg tctgatggtg
    47161 ggtctgggtg aaaccaatga agaaattatt gaggtaatgc gcgacctgcg ccgtcatggt
    47221 gtgacgatgt taacgctggg gcaatatttg cagccaagcc gccatcacct gccggttcaa
    47281 cgttacgtta gcccggatga gttcgacgaa atgaaagccg aagcgctggc gatgggcttt
    47341 acccatgctg catgcggtcc gtttgtccgc tcttcttacc acgccgattt gcaggcgaaa
    47401 ggaatggaag ttaagtaatc ctgtaataat tacttacatc tgtctgataa aaaacccgct
    47461 ttttgaagcg ggttttttgt atcgaacaag atattgaggg agcgtcctgc tcgccacgtc
    47521 actctttatg agagagcttt tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag
    47581 catcgtcatc attcatcgcc ttcttgaacc ctttaatggc cgctccaagg tctccgccca
    47641 gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag cgccgcaact accagcagtt
    47701 tggtaatact aatctcaccc atagatacct tcttgacata taaccggcaa cagaacgcgc
    47761 cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc acacaatagc aatctgtaac
    47821 aaggtgaatc aagcgccgag taaaaaaaca tcataataat tgcggcggcg caaagcgacg
    47881 gttgtttaag atgggcagtt gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc
    47941 cataatgagt gcaggcattt ctgacgctgc cgcaatggtg acgccaaagg gatctataat
    48001 ccggctttgc ccgatatttt tgttcccgca ctcccccgcc gccaccatat aacaggtggt
    48061 atccagcgca cgagcggcaa gcaacgttga ccagtgatgc tctttgagcg gcccacgaac
    48121 ccaggcggca ggaagtacca ggatttcagc tccctgtaaa gcctgtgcca gcgccagctc
    48181 tggaaagcgt aagtcataac aggtcatcag accgaccttc atcccttcca cctccagtaa
    48241 cggagctatt tcattaccag catcaacatg gcgtgattcc tgaatggcaa atgcatcata
    48301 gagatgcagt ttggcataac gggcgacgat gtttcctgcc tgaagtgcca ccagcatatt
    48361 ccatgcgcgc cccggcgttg aaggaagatg aatcgtcaga attgtcgtca tcatgttacg
    48421 tttactttct cgccgtaaac gtccgaggaa ttcgccttcc agcagctgtg ctgatttaac
    48481 cgatagatct gcatcatgat catcgcgcgc cagcaatgct tccggcagga caaacagcga
    48541 tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa atctcagcgt tcttttccca
    48601 cacagatgta acagcaaact gtcctgcagc aaccagcatt gccacctcct gtcagagtcg
    48661 taaaattcgc caacgacgat tattcggcgt tacacttatc actcatacaa atcaaatagc
    48721 aggattttgc agtgttacaa cttcttttag cagtttttat tggcggtggt acgggaagcg
    48781 tagcgagatg gctgttaagt atgcgattta acccgctgca tcaggcgatt ccgttgggga
    48841 cgctggcagc aaacctgatt ggggcattca tcataggaat gggattcgcc tggttcagca
    48901 ggatgacgaa cattgatcca gtgtggaaag tattaatcac caccggattt tgtggcggtc
    48961 taacaacctt ctcaacattt tcggcagaag tggtgttttt gttacaagag ggccgctttg
    49021 gctgggcatt actgaacgtt ttcgtcaacc ttctggggtc ttttgccatg accgcactgg
    49081 cattctggct gttttcggcc tcaaccgtac actaaagacc aaaaaaaacc cgctgattaa
    49141 gcgggttttg aattcttgct gacgtatctt acagagcgat tacgtttgca gcagaagggc
    49201 ctttggcacc gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac
    49261 cattagtctg gattgcagag aagtgtacga acacgtcttt gctgccgtct tccggagtaa
    49321 tgaaaccgaa tcctttggac tcattaaacc acttaacgtt acctttaatc ttagacatca
    49381 aaattacctt tacatgaaaa atagacacaa atgctgtgtc ggttatcagt acaccaattg
    49441 tggtacgctt tgtccagtgc aactttacta aaaagtgata aaagtcgcgt tatttttgat
    49501 gttgcttatt ttagttattg attttaaaga agttactaaa acttcatttg tctcaaaact
    49561 gaaagcgcat ccaggcaaag tacacattgc cattgttgta ggtacccgga atgtaggtca
    49621 tctgaaaagt cactgggcca taacccacgg aggccaatgg cagtagaacc gggagaggga
    49681 tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa tcccagacct aaatgaaaat
    49741 tttcatccgc cagcggtcgc caggtacttt cccatccgta tccggcaatc ggttcccatt
    49801 tgttccacga gtccttaaat gccatggcat acaggccatg ccagtttcct ttttcatccc
    49861 aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt atagcgatcg gttttttctt
    49921 tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat atataaatca taatgttcag
    49981 gctgttgcca ggtttgtgca atattttctc taaacgttgt catcctctca tctgcgttag
    50041 caaaaacttt accaacgctg attaactgaa taaaaacaaa ggaaaagata gcgacatatt
    50101 tactcacgtt catttgtgac cataaaacat ttatcaaaaa tctactacta gcatagcaaa
    50161 gctacaatta acataacctt aatagaccca acataaagaa taatctgaat agctgctcta
    50221 tttatttccg cttgttcaag ttaaatattt ccgcgcatcg attaaagatg atgcgtcgtt
    50281 ttctgcgatg ggaatagtca aaaaagaaaa accaagtctt ttttgatgac aaaatgcaat
    50341 caaggaa
//
LOCUS       DAZOYF010000011        46526 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZOYF010000011 DAZOYF010000000
VERSION     DAZOYF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.011"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            361..486
                     /gene="timP"
                     /locus_tag="ACHUOF_000643"
     CDS             361..486
                     /gene="timP"
                     /locus_tag="ACHUOF_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR0609950.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(518..1294)
                     /gene="sseB"
                     /locus_tag="ACHUOF_000644"
     CDS             complement(518..1294)
                     /gene="sseB"
                     /locus_tag="ACHUOF_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR0609951.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1436..2719)
                     /gene="pepB"
                     /locus_tag="ACHUOF_000645"
     CDS             complement(1436..2719)
                     /gene="pepB"
                     /locus_tag="ACHUOF_000645"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR0609952.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2778..2978)
                     /gene="iscX"
                     /locus_tag="ACHUOF_000646"
     CDS             complement(2778..2978)
                     /gene="iscX"
                     /locus_tag="ACHUOF_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR0609953.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(2990..3325)
                     /gene="fdx"
                     /locus_tag="ACHUOF_000647"
     CDS             complement(2990..3325)
                     /gene="fdx"
                     /locus_tag="ACHUOF_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR0609954.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3327..5177)
                     /gene="hscA"
                     /locus_tag="ACHUOF_000648"
     CDS             complement(3327..5177)
                     /gene="hscA"
                     /locus_tag="ACHUOF_000648"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR0609955.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5194..5709)
                     /gene="hscB"
                     /locus_tag="ACHUOF_000649"
     CDS             complement(5194..5709)
                     /gene="hscB"
                     /locus_tag="ACHUOF_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR0609956.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5805..6128)
                     /gene="iscA"
                     /locus_tag="ACHUOF_000650"
     CDS             complement(5805..6128)
                     /gene="iscA"
                     /locus_tag="ACHUOF_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR0609957.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6145..6531)
                     /gene="iscU"
                     /locus_tag="ACHUOF_000651"
     CDS             complement(6145..6531)
                     /gene="iscU"
                     /locus_tag="ACHUOF_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR0609958.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6559..7773)
                     /gene="iscS"
                     /locus_tag="ACHUOF_000652"
     CDS             complement(6559..7773)
                     /gene="iscS"
                     /locus_tag="ACHUOF_000652"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR0609959.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7885..8373)
                     /gene="iscR"
                     /locus_tag="ACHUOF_000653"
     CDS             complement(7885..8373)
                     /gene="iscR"
                     /locus_tag="ACHUOF_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR0609960.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8643..9383)
                     /gene="trmJ"
                     /locus_tag="ACHUOF_000654"
     CDS             complement(8643..9383)
                     /gene="trmJ"
                     /locus_tag="ACHUOF_000654"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR0609961.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9502..10305
                     /gene="suhB"
                     /locus_tag="ACHUOF_000655"
     CDS             9502..10305
                     /gene="suhB"
                     /locus_tag="ACHUOF_000655"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR0609962.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            10450..11304
                     /locus_tag="ACHUOF_000656"
     CDS             10450..11304
                     /locus_tag="ACHUOF_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0609963.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            11495..12775
                     /gene="csiE"
                     /locus_tag="ACHUOF_000657"
     CDS             11495..12775
                     /gene="csiE"
                     /locus_tag="ACHUOF_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR0609964.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12767..13906)
                     /locus_tag="ACHUOF_000658"
     CDS             complement(12767..13906)
                     /locus_tag="ACHUOF_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR0609965.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14066..14956)
                     /gene="hcaR"
                     /locus_tag="ACHUOF_000659"
     CDS             complement(14066..14956)
                     /gene="hcaR"
                     /locus_tag="ACHUOF_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR0609966.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15092..16453
                     /gene="hcaE"
                     /locus_tag="ACHUOF_000660"
     CDS             15092..16453
                     /gene="hcaE"
                     /locus_tag="ACHUOF_000660"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR0609967.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16450..16968
                     /gene="hcaF"
                     /locus_tag="ACHUOF_000661"
     CDS             16450..16968
                     /gene="hcaF"
                     /locus_tag="ACHUOF_000661"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR0609968.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            16968..17288
                     /locus_tag="ACHUOF_000662"
     CDS             16968..17288
                     /locus_tag="ACHUOF_000662"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR0609969.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17285..18097
                     /gene="hcaB"
                     /locus_tag="ACHUOF_000663"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             17285..18097
                     /gene="hcaB"
                     /locus_tag="ACHUOF_000663"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR0609970.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18107..19309
                     /gene="hcaD"
                     /locus_tag="ACHUOF_000664"
     CDS             18107..19309
                     /gene="hcaD"
                     /locus_tag="ACHUOF_000664"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR0609971.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            19406..19828
                     /locus_tag="ACHUOF_000665"
     CDS             19406..19828
                     /locus_tag="ACHUOF_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0609972.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19876..20748)
                     /locus_tag="ACHUOF_000666"
     CDS             complement(19876..20748)
                     /locus_tag="ACHUOF_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR0609973.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20760..21854)
                     /locus_tag="ACHUOF_000667"
     CDS             complement(20760..21854)
                     /locus_tag="ACHUOF_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR0609974.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(21887..22885)
                     /locus_tag="ACHUOF_000668"
     CDS             complement(21887..22885)
                     /locus_tag="ACHUOF_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0609975.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22910..24421)
                     /locus_tag="ACHUOF_000669"
     CDS             complement(22910..24421)
                     /locus_tag="ACHUOF_000669"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR0609976.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24444..25426)
                     /locus_tag="ACHUOF_000670"
                     /pseudo
     CDS             complement(24444..25426)
                     /locus_tag="ACHUOF_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(25523..28804)
                     /locus_tag="ACHUOF_000671"
                     /pseudo
     CDS             complement(25523..28804)
                     /locus_tag="ACHUOF_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            28922..30115
                     /locus_tag="ACHUOF_000672"
     CDS             28922..30115
                     /locus_tag="ACHUOF_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFR0609977.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(30313..31566)
                     /gene="glyA"
                     /locus_tag="ACHUOF_000673"
     CDS             complement(30313..31566)
                     /gene="glyA"
                     /locus_tag="ACHUOF_000673"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR0609978.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            31893..33083
                     /gene="hmpA"
                     /locus_tag="ACHUOF_000674"
     CDS             31893..33083
                     /gene="hmpA"
                     /locus_tag="ACHUOF_000674"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR0609979.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33128..33466)
                     /gene="glnB"
                     /locus_tag="ACHUOF_000675"
     CDS             complement(33128..33466)
                     /gene="glnB"
                     /locus_tag="ACHUOF_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR0609980.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(33527..34861)
                     /gene="glrR"
                     /locus_tag="ACHUOF_000676"
     CDS             complement(33527..34861)
                     /gene="glrR"
                     /locus_tag="ACHUOF_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR0609981.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(34851..35564)
                     /gene="qseG"
                     /locus_tag="ACHUOF_000677"
     CDS             complement(34851..35564)
                     /gene="qseG"
                     /locus_tag="ACHUOF_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR0609982.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(35729..37156)
                     /gene="qseE"
                     /locus_tag="ACHUOF_000678"
     CDS             complement(35729..37156)
                     /gene="qseE"
                     /locus_tag="ACHUOF_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR0609983.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(37714..41601)
                     /gene="purL"
                     /locus_tag="ACHUOF_000679"
                     /gene_synonym="purI"
     CDS             complement(37714..41601)
                     /gene="purL"
                     /locus_tag="ACHUOF_000679"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR0609984.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            41860..43416
                     /gene="mltF"
                     /locus_tag="ACHUOF_000680"
     CDS             41860..43416
                     /gene="mltF"
                     /locus_tag="ACHUOF_000680"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR0609985.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(43413..43916)
                     /gene="tadA"
                     /locus_tag="ACHUOF_000681"
     CDS             complement(43413..43916)
                     /gene="tadA"
                     /locus_tag="ACHUOF_000681"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR0609986.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(43974..44609)
                     /gene="yfhb"
                     /locus_tag="ACHUOF_000682"
     CDS             complement(43974..44609)
                     /gene="yfhb"
                     /locus_tag="ACHUOF_000682"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR0609987.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            44818..45666
                     /locus_tag="ACHUOF_000683"
     CDS             44818..45666
                     /locus_tag="ACHUOF_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR0609988.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46005..46526)
                     /locus_tag="ACHUOF_000684"
                     /pseudo
     CDS             complement(46005..46526)
                     /locus_tag="ACHUOF_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11066 a        12790 c        11694 g        10976 t
ORIGIN      
        1 atattactta aacatgtaaa tttactttcc tttaaaaaac aaaaaaccgc caaaatcagg
       61 cggttatttg ttgctggtcc ggttcgcggc ctttccagca ggttgtatta ccgtagtaat
      121 gcaagcgcgt ctcagcggag acaatactcg ccagtaactc tctttttgtc aagcaaaaga
      181 gagttattat tgttctgtta gtgtattatc cactgcggcc ctttccgccg tctcgcaagc
      241 gggcgctggc ttaaggaaag gatgttccgc gaccgttaga gcaagcgcgt ctcagcgctt
      301 gctatcgcgg caatatcgcc agtggtgctg tcgtgatgcg gtcttcgcat ggaccgcaca
      361 atgaagatac ggtgcttctg tatcgtactt attgtttctg gtgcgctgtt ttcccaagta
      421 aataataacc ggagtctctc cggcgacaat ttactggtgg ttaacaacct tcagagcagc
      481 aagtaagccc gaatgccgcc ctttagggcg gcatatttta gattatccgg ttttgtttaa
      541 agtcacgcaa aaaaccaccc cagcgacgtt catagaatgg cgcaatatgt tcggtaataa
      601 agtggctaat tcctttttcc ccttttttca cctgacaaat atcgattggt tcatcgccag
      661 gtaatgtatc ggtcgctaca cttcccgtcg cctgaataat ttcttcgata tcaccatcgg
      721 cttcaatgcc aataagtaaa ttaggctgtg cctcttcgtt ctctttaatt gaacaaataa
      781 aagcacgctt caccggctta atggttttaa ataaggtggt gagtgaatca atcatttgtg
      841 ctggcggctc tgcgacttcc gataatatca gcgattcacc gccttccagg atttcctggc
      901 tgctcagcgg atttccctct tcaccaatca acaaactgat ttcacgcggc ataaattctt
      961 taccggtcgg cagtttggca ttaaggaaga gcgtttcgcc aagggtcatc tcaaacaatg
     1021 tgcgaacggg cattacgaca aatgcctgtt cgtcttcaac cgcctgttga agcgcttcta
     1081 acgaggtgaa aaaaggaatg acgctggtgc cgtcttcttt ttcccagtgc tgtaaatcaa
     1141 gcgcactatc ttcaaccaca gcctcgccct gcgccgccgt accaggcacc cagacggtgg
     1201 attccagtag agtacggaaa aaggccgggc ggtgcgccgg ttcggttgct gctttttcca
     1261 gcaggtcttc aagttcgttt tttgtttcgg acataagaac cacaattcat tcaacgttcg
     1321 gggcgcaaat gcctgatgcg ctacgcttat caggcctaca agggattcgc aatttgttga
     1381 atttgcagaa tttgtaggcc ggataaggcg tttacgccgc atccggcata ttagtttacg
     1441 ccgttaacag attagctatc gtgcgcacac caagtcccgt agcgcccgca gaccactgtt
     1501 caaccggcgc tttacggtaa gtcgccgagc agtcgatatg cagccagcct tgctgatagt
     1561 tctcaacaaa gtgcgacagg aagcccgccg ccgtgctcgc gcctgccgga tacgccgcgc
     1621 ttccggtatt gttcagttcg gcaaagttag acggcagctg gctgcggtgg aattccgcca
     1681 gcggcagacg ccagaatggt tcgttctctt gtgaggcact cgccagtaaa cgaccggcaa
     1741 gcgcatcgtc aaaactgaac agcgcgtgat aatcattacc cagcgcagtt ttcgccgccc
     1801 cggtgagggt cgccgcatca atgatcattt ccggtttctg cgcactggca tcaatcagac
     1861 catcggcaag caccagacgc ccttcggcat cagtgttcat cacttcaact tttttaccgt
     1921 tgcgataggt gatgatatcg cccagcttga acgcattgcc gctaatcagg ttatccgcac
     1981 agcagaggaa cagcttcacg cgcttgttca gtccgcgcat aatggcaaat gccagcgccc
     2041 cggtaaccgt tgccgcgccg cccatgtccg acttcatcga gtccataaac gcagtctgtt
     2101 tgatgctgta gccgccggag tcaaaagtga tacctttacc taccaggcac gcgtacaccg
     2161 gtgcttcttt atcgccagtt gggttgtaat ccagcgccag caataccgga gaacgttctg
     2221 aaccgcgccc gacagtgtgc agccccatat aaccttgctc acgcagatct tcgcctttgg
     2281 tgatccgata agtcacacga tcgcccgcga cgttgctgat cagatcaaca gcacgctgtg
     2341 ccagttgcga tggtcccaat tcttctgccg gtgcgttgat ggtgtcacgc acccagtcga
     2401 tgatcatcag gcggttatcc agttcctggc gctgggcatc gtccagatcc ggccactcca
     2461 ctttacgcgt gcctttcggg cctttataac cttgccagaa tgcccagcag cgatccgcat
     2521 cccagccttc accgcttaac tgaacatgct tgatgcccag accgtcaatc ttgcgggccg
     2581 cacgctggat cagccccaga tcgtctgccc cgttcaaatg cagggtaatg ccgtcattat
     2641 taatgctgta agttgctttt tctccccagc gcgcgtcggc aggttgggta gagagggtaa
     2701 tcttcatcgc ttctgtcatt ttagttatcc ttattcgttt aacagcaaaa cgggctaccc
     2761 ccaggcagcc cgttcattca ctcggcttcg tccagccaga ctaacaaaat cgcttcgagg
     2821 attttctcgt tggatgcctg cgggtcgtcg tcgaaatctt ccagatcgca aatccactga
     2881 tgcatatcgg tgaatcgaac cgttttcgga tcaagatcgg gatacgcatc gtacagtgct
     2941 tcgccaattt cgcggctatc ggtccactta agtcccatac taacctctgt taatgctcac
     3001 gcgcatggtt gatagtgtaa cgcgggattt cgaccactaa atcttcgtcg gtgacgcgcg
     3061 cctggcagct taaacggctt tccggctcca gtccccaggc tttgtccagc atgtcgtctt
     3121 cctgctctga gctttccggc agtgagtcaa aaccttcacg aacgatgcag tggcaggtgg
     3181 tgcaagcaca ggatttttca caggcgtgtt caatctcaat accgttacgc agcgctgcgt
     3241 cgagaatggt ttcaccgcta ttagcttcca gaacagcgcc atcagggcag aggtcctgat
     3301 gaggcaaaat aacaatcttt ggcatattaa acctcgtcca cggaatggcc tttcagcgca
     3361 cgacgaaccg actggtccat gcggcgagcg gcgaaatcct gggtttgttt gtctacgttt
     3421 ttaatcgctt gttcgatggc gtcaacatca tcgccctgcg ccacttcact caggtgagcg
     3481 gcagcattgt caatgacctg acgctctgcg gcgcttaaca gcgcggcatc agcagccagc
     3541 gcgccgtgca gactttccag cacacgcgcc gcttctactt tttgttctgc cagcatacgg
     3601 gcttttacgt cctgctcggc atagctcatt gagtctttga tcatcgaagc gatttcgcta
     3661 tcggtcagac cgtaagacgg tttgacctga atagacgcct caacgccggt ggatttctcc
     3721 atcgccgtca cgctcaaaag accgtcggca tcgacctgga acgtcacgcg aatatgcgca
     3781 ccgccagccg gtagcgccgg aataccacgc agcgcaaaac gcgccagtga gcggcagtcc
     3841 tgcaccagtt cgcgctcacc ctgcattaca tggatagaca tcgccgtctg accatcttta
     3901 aaggtggtga aatcctgagc gcgggccacc ggaatagtgg tattacgcgg aatcactttc
     3961 tccaccaggc cgcccatcgt ttcgaggccc agcgacagtg ggatcacatc aagcagcagc
     4021 atttcgctgt ctggcttgtt acccaccaga atatccgcct gaatcgccgc gccaatagcg
     4081 acgactttat ccgggtcgat ggaagtcagc ggtggacgac cgaaaaattc gcctacccgt
     4141 tcacgcacca gcggcacgcg agtagaaccg cccaccatca ccacttccag cacttcgtca
     4201 gcttctacac ccgcgtcttt cagcgcgcga cgacaagcca gtaaggttcg ttttaccagt
     4261 ggcgcgatca gttcattgaa ttgttcacgg ctgatttcgc cctgccagcc cgcaacgtta
     4321 acggtcacgg agtccgcatc gctcagcgcg attttggctg caatggtggc atccagcagt
     4381 tcacgctgaa cgcggttatc gctacgatca ggaatgtccg cctgctcgcg aatgtaatcc
     4441 gccagcagat ggtcgaaatc atcgccgccg agcgcggaat caccgccggt tgccagcact
     4501 tcaaacacgc cgcgacttaa gcgcagaatg gaaatatcaa acgtcccgcc accgaggtca
     4561 taaacagcga tcacgccttc ctgaccggaa tccagcccgt aggcgatagc cgcagcagtc
     4621 ggttcgttaa gtaagcgcag aacatgcaac cccgccagac gcgccgcgtc tttggtgccc
     4681 tgacgctggg catcgtcaaa gtacgccgga acggtgataa ctacaccatc cagctcgcct
     4741 gccagggctt cagttgcccg cgccgccagt gctttgagga tgtccgcaga aacgcgcacc
     4801 gggttcagta gccccgccgc cgtttcaatc atcggcaggc cgttttcgct ggcctggaat
     4861 tgataaggca gatgcggata gcgttgctgg atatcagcca gcgagcgccc catcaggcgt
     4921 ttaacagaac taatggtgtt ggcggtgtca agcgctgcgt tagtacgcgc gtcataaccc
     4981 accgaatgcc cttgctgttg atagtgaaca acagatggca gcaggtgacg gccttcatgg
     5041 tcggctaacg tttcggcctg accgctgcgc actgtcgcca ccagcgagtt ggttgtgccc
     5101 aggtcaatac cagccgccag acgacgctga tgcggcgcag cactcaaacc aggttcacta
     5161 atttgtaata aggccatgtt tagcttccag aaattaaaaa tcgagcagtt tttcttcgag
     5221 ttgttcggca ctgctccgca gtttatcgag aaaacgcagc ttacgcaccg tatccgccgc
     5281 cgcgtcccac gcctcgttgt ctaactgttc aaccatcaac tgatggcggg tatcaaacat
     5341 ctttttcaca cgtttgataa agctttccag ccgcgcttca tctttcgcct gttcgatctc
     5401 gtccagctct tcgcgcagct ccaactgttc catcaggaac gcggtgtcgc gcacagtatg
     5461 ctgctcgctg gcgagatcaa aaccgtgtaa agaaagcaaa tattccgcgc gcattaacgg
     5521 atgacgcagc gtttgccagg cctggttaat ggttgcagat tgctgtacgg cggcgagttg
     5581 ttccgccagg cttccgctgg cgaatttatc aggatgatac tgacgttgta gatcctgaaa
     5641 acgcaggctc agtgcctggg tatcgagttg ataacgggca ggcaagccaa agagggtgaa
     5701 gtaatccata acattctcag ggctgcgatc cgccgcgata acggcgaatc aggtaaaaca
     5761 aaccccacgc gcaggcgacc acggtggggt tatcggtatg cgcatcaaac gtggaagctt
     5821 tcgccgcaac cacactcatc tttaacgttc gggttggtga atttaaaccc ttcattcagg
     5881 ccttctttta cgaagtccag ctgcgtaccg tccagaaatt gcaggctttt gccatcgacc
     5941 acgactttca cgcctttgtc ttcaaacacg atgtcttccg gcgtcggttc gtcaacaaat
     6001 tccagtacat aagccatacc tgaacatccg gaggttctca cgcccagacg caggccaaac
     6061 cctttgccgc ggttagccag aaaggtattt actcgcgctg ctgcactgtc gctcagtgta
     6121 atcgacataa ccaaacctca actcttattt tgcttcacgt ttgcttttat agtccgcaat
     6181 ggcggctttg atcgcgtctt ctgccagaat agaacagtga attttcaccg gcggcagttc
     6241 aagttcttca gcaatatcgg tgtttttgat cgcctgcgct tcgtcgagag acttcccttt
     6301 cacccattcg gtgaccaggg agctggaagc gatagcggaa ccgcagccgt aagttttaaa
     6361 acgcgcgtct tcaatgatac cttcatcgtt gactttaatc tgcaacttca ttacgtcgcc
     6421 acaggccggt gcacccacca tgccgctgcc gacgttctcg tcgttgttat caaaggaacc
     6481 cacgttacgc ggattctcgt aatggtcgat aactttttcg ctgtaagcca ttataaattc
     6541 tcctgattcc gataccgatt aatgatgagc ccattcgatg ctgttcagat ccacgccctg
     6601 cttgtacatt tcccacagcg gagaaaggtc acgcagacga ccgatggatt tacgaactaa
     6661 ctcgatggtg tagtcgatct cttcttcagt agtaaaacga cctaaagaga aacggataga
     6721 gctatgtgcc agctcgtcgt tcagccccag cgcgcgcagc acgtaggacg gttcgaggct
     6781 tgctgacgta caggcggaac ctgaagaaac tgcgaggtct ttcagcgcca taatcagcga
     6841 ctcaccttca acgtagttga agctgacgtt gagaatgttc ggcgcaccgt gttccaggtc
     6901 accgttcagg taaacttctt cgatatcttt gatgccgttc cacagacggt tacgcaggcc
     6961 gcgcagacgt tccatctcgg tcgccatctc ttcttttgcg atgcgatagg cttcgcccat
     7021 tccgacgatc tggtgaacag gcagagtgcc ggaacgcata ccgcgctcgt gaccgccgcc
     7081 gtgcatttgc gcttcgatgc gtacacgcgg tttacgacgc acatacagcg caccgatacc
     7141 tttcgggcca tagattttgt gaccggagaa agacatcagg tcaactttca actggctcag
     7201 gtcgattggc agtttgccca cgctctgggt tgcatcaacg tgatagataa tgccacgagc
     7261 acggcacatt tcgccgatag ccgcgatatc ctgcaccacg ccgatttcgt tatttacgtg
     7321 catgatggaa acaaggatgg tgtcgtcacg catcgccgct tcgagttctt tcaggtcgat
     7381 aataccgtta cgctgcggtg ccaggtaggt gacttcgaaa ccttcgcgct ccagctgacg
     7441 gcaagtatcc agtaccgctt tgtgttcggt tttgctggtg atgatgtgct tgcctttttt
     7501 ctgataaaag ttggctgcac ctttgatcgc caggttgtca gattcggttg caccagaggt
     7561 aaagacgatt tcacgcggat cagcgccgac cagatcggca atctgattac gggcgatatc
     7621 taccgcttct tcagcctgcc agccgaaacg gtgagaacga gaggccgggt taccaaaggt
     7681 tccgtccatc gtcatgaact gcatcatttt ctcggcaaca cgcgggtcca ccggcgtggt
     7741 tgcggagtag tcgagataaa tcggtaattt cattgctcta taaactccgt acatcactca
     7801 atgcaaggaa tcaggctacc ggctggatgt acgaccgtgt ttacggagta tttagcactc
     7861 cggcctgatt ctgaattctt tttattaagc gcgtaactta acgtcgatcg cgtcttgtgt
     7921 gcgggtgcgt ggcgcgtcgt gagtatgctg acgaccagac acatccagca cttcctggtt
     7981 attaaccagt tcgcctaaag taatgttgtt gagaaaaccg gtgagacggt cgctcaaatc
     8041 acgccacagc gcgtgggtca ggcatttatc gccgccctgg cagccgcctt taccctgaca
     8101 acgggtggca tctacagatt cgtcaacggc gctaatcact tcgccaacgg cgatgctgct
     8161 ggcatctttg cctaacagat aaccaccgcc tggtccacgt acgctggaaa ccagaccatt
     8221 tttacgcaga cgggaaaaca gttgttccag ataagaaagg gaaattccct gacgttcgga
     8281 aatatcagcc aacggtaccg ggcccgcttc agagttgagc gcaacgtcaa gcattgcggt
     8341 cacggcatag cgccctttag atgtcagtct catgtcttac ttcacctcaa actcgcccct
     8401 gcccggggtt ttttattgta aaagtggggg tattgcatag cagggtcaag tctgacattc
     8461 ccgagtaaat tggtcaacta tttacttgac tgatttagtc gggtatttaa ccttcagtgc
     8521 cattttttta tcgtggcgtt tgtacctgtt gtcggatgcg gtgtgaacac cttatccgac
     8581 ctacgattct gtacattgta ggcctgataa gacgcgttaa gcgtcgcatc aggcaaccac
     8641 tgttattcgg ccttgttacc tttattctgc tgctcaatag aagccagaat cccgcgcagg
     8701 atattcaatt cctggctttc cgggcgcgca cgggtaaaca gacggcgcaa tttattcatc
     8761 acctgccccg gatggttttc acggataaaa ccggttgcca gcagcgtttg ttccagatga
     8821 ccataaaaac gctccagatc atcgaccagc ggatacggcg tctcttcatg ctcgacctgc
     8881 tcgccatttt cctgagttgc cagccaggcc atacgcactt cataggcaat aacctgaacc
     8941 gccattgcca ggttcagcga actgtattcc gggtttgccg caatcgcgac atgataatgg
     9001 catttctgca actcttcatt ggtcaagccg acgcgctcac gaccaaacac cagcgccacc
     9061 ggggtatttg ccgcttcagc gacgcttttc agaccgcatt cgcgcgggtc gagcatcggc
     9121 cacggcagcg tgcgggagcg tgcgctggtg cccaccacca ggctacaacc ggctaacgct
     9181 tcgtcgagcg tatcgacgat gtgagcatta ccaatcacat cgctggcccc tgcagccagg
     9241 gcaatcgcct gggagtcggg tttcaccagt ggattaacca gccacagatt ggttaatccc
     9301 attgttttca tggcacgggc aacagaaccc atattgccgg tgtgtgacgt ctccaccagc
     9361 acaattcgaa tattttgcag cattgtcttt cttcatctaa agattattca cgcatcttat
     9421 cataaaacga agacagatgc cgatctcgct gctatactct gcgccgtttt cccgttcttt
     9481 aacatccagt gagagagacc gatgcatccg atgctgaaca tcgccgtgcg cgcagcgcgc
     9541 aaggcgggta atttaattgc caaaaactat gaaaccccgg acgctgtaga agcgagccag
     9601 aaaggcagta acgatttcgt gaccaacgta gataaagctg ccgaagcggt gattatcgac
     9661 acgattcgta aatcttaccc acagcacacc atcatcaccg aagaaagcgg tgaacttgaa
     9721 ggtactgatc aggatgttca atgggttatc gatccactgg atggtactac caactttatc
     9781 aaacgtctgc cgcacttcgc ggtatctatc gccgttcgta tcaaaggccg caccgaagtt
     9841 gctgtggtat acgatcctat gcgtaacgaa ctgttcaccg ccactcgcgg tcaaggcgca
     9901 cagctgaacg gctaccgtct gcgcggtagc accgctcgcg atctcgacgg taccattctg
     9961 gcgaccggct tcccgttcaa agcaaaacag tacgccacta cctacatcaa catcgtcggc
    10021 aagctgttca acgaatgtgc agacttccgt cgtaccggtt ctgcggcgct ggatctggct
    10081 tacgtcgctg cgggtcgtgt tgacggtttc tttgaaatcg gtctgcgtcc gtgggatttc
    10141 gcggcaggcg agctgctggt tcgtgaagcg ggcggcatcg tcagcgactt caccggtggt
    10201 cataactata tgctgaccgg taacatcgtt gctggtaacc cgcgtgttgt gaaagccata
    10261 ctggcgaaca tgcgtgacga gttaagcgac gctctgaagc attaatgact caggcgggtg
    10321 atatcactca cccgccctcg cctttcaggc gctattccga aatacttcct caccgcttta
    10381 ctttctttcg tcactctccc accatctttc cccgattaat ggatgataaa gaagtcgcaa
    10441 ggaaaggtta tggcactgcc agtgaacaaa cgcgttccca aaattctgtt tattctcttt
    10501 gttgttgcct tctgcgttta tttagtgccg cgcgttgcca tcaacttctt ctattatcct
    10561 gacgataaaa tttacggtcc cgacccctgg tcggcggaat ccgtcgaatt tacggctaag
    10621 gacggtactc gtctgcaagg ctggtttatt ccttcttcga cgggccctgc tgacaacgcc
    10681 atcgcaacca tcattcatgc tcacggcaat gccggaaata tgtccgccca ctggccgctg
    10741 gtcagttggt tacctgagcg taatttcaac gtttttatgt ttgattatcg cgggtttggt
    10801 aaatcaaaag gcactccgtc ccaggccgga ttgctggacg atacgcaaag cgccatcaat
    10861 gtggtgcgcc atcgcagtga tgtaaaccca caacagctgg tgctgttcgg gcagagtatt
    10921 ggcggggcga atattcttgc tgttattggt cagggtgatc gtgaaggcat acgtgcggtg
    10981 atcctcgact ccacatttgc ctcttatgca accatcgcca accagatgat ccccggcagt
    11041 ggctacttac ttgatgagag ttacagcggc gaaaattaca tcgccagcgt cagcccgatc
    11101 ccgcttttac tcattcacgg taaagccgat cacgttatcc catggcagca cagcgaaaag
    11161 ttgtatagcc tggcaaaaga gccaaaacgg ttgatcctaa tcccggatgg cgaacacatt
    11221 gatgcttttt ccgatcgtca cggcaatgtt tatcgcgaac agatggtgga ctttatcctt
    11281 agcgcgttga atccgcagaa ctaacccatg atcgctagca cgataatcat tcacaaaacc
    11341 accttaagac atgctaatcc actggtcaga acagtttaaa atgagaaaaa ttctgtgacg
    11401 cttgccaaca tttctgatga ttagcattcc cttcgccatt tccctgagca aactttagct
    11461 attcttatca attatgctta tgggagatct acagatgatg cctacgcttg ctccaccatc
    11521 tgtcctttcg gctccccagc gccgctgcca gatcttgctg acgctctttc agccggggtt
    11581 gaccgccacc atggcaacct tcagcgagct taatggtgtg gatgatgata ttgccagtct
    11641 tgatatcagc gaaacaggac gggagatcct gcgctatcat caactcacac tgacgactgg
    11701 ttatgacggt agctaccggg ttgaaggtac agtgcttaac caacgtttgt gtttatttca
    11761 ctggctacga cgtggtttcc gtctgtgtcc gtcatttatt accagccaat tcacccccgc
    11821 cctgaagagt gaactgaagc ggcgcggaat tgcgcgtaac ttttacgacg ataccaatct
    11881 acaagcgtta gtgaatctct gctcccgacg gctgcaaaaa cgctttgaat cgcgcgatat
    11941 tcatttcctg tgtctgtatc tgcaatattg tttgctgcag caccacgctg gaattacgcc
    12001 tcagtttaat ccgctccaac gtcgctgggc ggaatcctgc cttgaatttc aggtagcgca
    12061 ggaaattgga cgccactggc agcgtcgggc gctccagcat gtaccgcctg atgagccact
    12121 gtttatggca ttactttttt ccatgttgcg ggttcccgat ccactgcggg atgcgcatca
    12181 gcgggacaga caattgcgtc agtctatcaa acgtctggta aaccattttc gtgagctggg
    12241 aaatgttcgt ttttatgatg aacaggggtt atgtgatcag ctttatatcc acctcgccca
    12301 ggcgttaaat cgcagtttgt ttgccatcgg tattgataat accctgccgg aagagttcgc
    12361 cagactgtat ccacgcctgg tacgcaccac ccgcgcggcg ctggccggat ttgaaagtga
    12421 atacggcgtc catctttctg atgaggaaag tggtctggtc gcggtgattt tcggtgcctg
    12481 gctaatgcag gaaaacgatc tgcatgaaaa acagattatt ctactgaccg ggaatgatag
    12541 cgagcgagaa gcgcagattg agcagcagtt acgcgaacta acgttactgc cgctcaacat
    12601 taagcatatg tcggtaaagg catttttgca gacaggcgct ccgcgtggcg cggcactgat
    12661 tattgcgcct tataccatgc cgttaccgct cttttcacca ccgctgatct atacggacct
    12721 gacgctgaca acacatcaac aggagcagat ccgcaaaatg ctcgaatcag catgagggaa
    12781 caactttcgg gcgcaaaaac attgccggaa gcgccaccag cgccattacc cagaacacgc
    12841 cgtggcccag atattgatac aggaaaccgg cgaaaacggt catgatagcg atactgccgc
    12901 ccatcgcgac ggcagagtaa accgcctgta aacggatgac ttcgctaacc tggcgagcag
    12961 caatataacg catggcggcc aggtggcaga ccgtgaaggt gccgcaatgc agaatttgca
    13021 ccactatcaa ccacggcaac gccgtagttg ctcccataat gccccagcgc actacgccgc
    13081 aaatcgccga gatcaacagc atatcgcgag cactacaacg gcggaaaagt ttattactca
    13141 gcgcaaagat aatgacttcc gccaccacgc ccagcgacca caaatacccc accgccgagg
    13201 ccgagtagcc agctgcctgc cagtaaatgg cgctaaaacc gtaataggcc gcatgtgccc
    13261 cctgcaataa acaaacgcag gccagaaagc gccagttctg gcgaaccagc gccaaccacg
    13321 cagaccaacc ggtgctctcc tgctggcggc ttgccccttg tggctgaatc gtcggacgga
    13381 tgagaaagcc gagcagcatg gatgccacgc ccaacgtcaa cagcacgagg atcacccgat
    13441 aatcaaacat agtgaccagt ttgcccgtca gcgccgagcc aatgacaaac gccaccgagc
    13501 cccacagtcg cactttgccg taatcaagcg ggaactgctt ttgccacgta ttcgccagtg
    13561 catcggtcaa cggtaccagc ggtgagaaaa agaggttaaa gccaatcatc accagcatca
    13621 gccacgctac gtgcgccccc gcccagaagg cgacagcaaa gagaagtggc agcagtgcca
    13681 gcacgcgcaa ggcggaaatc aggcgggaag gatcgctgac gcggggcgcg atgagcaaac
    13741 tcccgagaaa acgggcaacc agacctgccc ccaataacag gccgatggtt tctggcgtta
    13801 aaccaatccc tttaagccag acgctccaga aaggtaaaaa aatgccgtaa ctaaaaaagt
    13861 atgtgaaata gccgagcgcc aaccagcgcg tggattgcaa aaccatgagt ccctcccgtc
    13921 aggaggcgtt agtctggggt taatcgaatg ttggtgcaag ttgaaaaata agaatattcg
    13981 agcctgcttc ccgatgtctg taacgccagt tcacaagcac taaaaaactg cgatccgcga
    14041 cgcatgttgc aaaaataatg cgtaattatg ccgttacgct tgccaaacgt tcctgcacaa
    14101 tggcgatgaa atcgcgtaat gccagcttca tctccccttt cttccacgcc atcagtaaag
    14161 caatggaagg cacatttccg gcaatgggac gaaaaacaac ctgcccggta ttaaaattat
    14221 tcatataacc gggtatcaaa gtgacgccca gccccatgcc caccagattc atggtcacca
    14281 gaatattcgt tgccacctgg acgatatttg gctggctgtt ttcttgcgca aaccacgctt
    14341 taacgatcgg cgcaagcgaa ccggaatacg ccggatcggt actgacgaaa ttcacgccat
    14401 ccagttgcgc ggcggtgatc tctttttcat gtgctaaagg gtgatcaacc ggtaacacca
    14461 ccactaatgg ttcgtcaaaa agctccagat aatcaatctc cgggctataa acaggatggc
    14521 gcattaagcc gacgtcgagt tcaccacggc gaattttttc ctcctgttgc gtggtgatta
    14581 aactcaccag ctcaatcaag gtgtctggct gtctgagacg aaacatcggt aatacttttg
    14641 gcaataaatt cacttccgcc gatggcacaa agccaatggt taattgtctg tcttcctgaa
    14701 caattttccg cgcccgtaat ttggcatttt ccgcttgttc gaggattgcc agcgcgtcct
    14761 ggagaaaaca ttctcccgcc gccgtcaacg cgactttgcg cttatccctc accagtaacg
    14821 gaacaccgac acagttttca agatcgcgga tctggctgct taacgaaggc tgtgaggtat
    14881 gcagtttttc cgccgcacgg gtaaagttca gtgcctgcgc cactgcgacg aaatagcgta
    14941 aatgccgtag ttccatcacc ttccccttgt tatcgaaaaa acgtctcagc tggtagaaat
    15001 taactatttc acatattagc aaccaaccag caacatcctt atggcacaaa aatagaaggt
    15061 caatacatct tatctttcag gattaaaaaa tatgaccaca ccctcagatt tgaacattta
    15121 ccaactgatt gacacccaaa acggtcgggt cactccgcgt atttataccg acccggacat
    15181 ttaccaactg gagcttgagc gtattttcgg tcgttgctgg ctatttctcg cccacgaaag
    15241 ccagatccca aaacccggtg atttctttaa cacctacatg ggagaagatg cagttgtcgt
    15301 ggtgcgccag aaagacggca gcatcaaggc gtttctcaac caatgccgcc accgggccat
    15361 gcgtgtgagt tatgcagatt gtggcaacag tcgcgccttt acctgcccgt atcacggctg
    15421 gtcttatggc attaacggcg agttgatcga tgtaccgctg gaacctcgcg cctacccaca
    15481 agggttgtgt aaatcccact gggggctaaa cgaagttcct tgtgtggaga gttataaagg
    15541 actgattttt ggcaactggg ataccagcgc accgggcctg catgattacc tgggtgacat
    15601 tgcctggtat ctggatggca tgctggatcg tcgcgaaggc ggcaccgaaa ttgtcggcgg
    15661 cgtacaaaag tgggtgatca actgtaactg gaaattcccg gcagagcagt tcgccagtga
    15721 ccagtatcat gctctgttca gccatgcttc tgccgttcag gtattagggg cgaaagatga
    15781 tggcagcgat aagcgcctcg gtgatggaca aaccgcccgc ccggtgtggg aaaccgccaa
    15841 agatgcgctg caatttggtc aggacggtca cggtagcggt ttcttcttta ctgaaaaacc
    15901 ggatgctaat gtctgggtcg atggcgcagt ttcaagctat taccgcgaaa cctatgccga
    15961 agcagaacaa cgtttaggtg aagttcgcgc cctgcgcctg gcgggtcata acaatatatt
    16021 ccccacgctt tcatggctaa acggcactgc cacactccgc gtctggcatc cgcgcggccc
    16081 tgatcaagtt gaagtgtggg cgttctgtat tactgacaaa gccgcctccg atgaagttaa
    16141 agccgctttt gaaaacagcg ccactcgtgc ttttggtcct gctggttttc tcgagcagga
    16201 tgactcggag aactggtgtg aaatccagaa attgcttaaa ggccaccgcg cccgcaacag
    16261 caaactgtgt ctggaaatgg ggcttggtca ggaaaagcgc cgcgacgacg gcattcctgg
    16321 cattactaac tatatctttt cagaaactgc cgctcgtgga atgtaccaac gctgggccga
    16381 tcttctgagt agcgaaagct ggcaggaagt gctcgataaa accgccgctt accagcagga
    16441 ggtgatgaaa tgagtgcgca agtttcactg gagttacatc accgcattag ccagtttctc
    16501 tttcacgaag ccagtctgct ggatgactgg aaatttcgtg actggttggc acagctcgac
    16561 gaagagattc gttacaccat gcgcaccacg gttaacgcgc aaacacgcga ccgtcgcaaa
    16621 ggcgtccagc cgccgacgac ctggattttc aatgacacca aagaccagct ggagcggcga
    16681 atcgcccgtc tggaaacggg catggcctgg gcagaagagc cgccgtcacg cactcgtcac
    16741 ttaatcagca actgccaggt aagcgaaacc gacatcccaa acgtatttgc cgtgagggtg
    16801 aattatctac tttatcgggc acaaaaagag cgcgatgaaa cattctatgt tggaacgcgt
    16861 ttcgacaaag ttcgccgtct ggaagatgac aactggcgct tgctggaacg ggatatcgtc
    16921 ctggatcaag cggtgatcac ttcccataac ctgagtgtac tgttctgatg aatcgaattt
    16981 atgcgtgtcc cgttgcggat gtgccggagg gtgaggctct ccggatagat acctcgcccg
    17041 tcatcgcctt gttcaacgtg ggcggcgagt tttatgccat taacgatcgt tgcagccatg
    17101 gtaatgcgtc gatgtcagaa gggtatctgg aagatgacgc cacggtggag tgcccgctac
    17161 acgccgccag tttttgcctg aaaacgggga aagcgttatg cctgcccgcc accgatccgc
    17221 tcaccactta tccagtacac gttgaaggtg gtgacatttt catcgactta ccggaggcgc
    17281 agccatgagc gatctgcata acgagtccat ttttattacc ggcggcggat cgggattagg
    17341 gctggcgctg gtcgagcgat ttatcgaaga aggcgcgcaa gttgccacgc tggaactgtc
    17401 ggcggcaaaa gtcgccagtc tgcgtcagcg atttggcgaa catattctgg cggtggaagg
    17461 taacgtgacc tgttatgccg attatcaacg cgcggtcgat cagatcctga ctcgttccgg
    17521 caagctggat tgttttatcg gcaatgcagg catctgggat cacaatgcct cactggttaa
    17581 tactcccgca gagacgctcg aaaccggctt ccacgagctg tttaacgtca acgtactcgg
    17641 ttacctgctg ggcgcaaaag cctgcgctcc ggcgttaatc gccagtgaag gcagcatgat
    17701 tttcacactg tcaaatgccg cctggtatcc tggcggcggt ggcccgctgt acaccaccag
    17761 taaacatgcc gcaaccggac ttattcgcca actggcttat gaactggcac cgaaagtgcg
    17821 ggtgaatggc gtcggcccgt gtggtatggc cagcgacctg cgcggcccac aggcgctcgg
    17881 gcaaagtgaa acctcgataa tgcagtctct gacgccggag aaaattgccg ccattttacc
    17941 gctgcaattt ttcccgcaac cggcggattt tacggggccg tatgtgatgt tggcatcgcg
    18001 gcgcaataat cgcgcattaa gcggtgtgat gatcaacgct gatgcgggtt tagcgattcg
    18061 cggcattcgc cacgtagcgg ctgggctgga tctttaagga agcacgatga aagaaaaaac
    18121 gatcattatt gtcggtggcg ggcaagcggc ggcaatggct gcggcctcgc tacgccagca
    18181 agggttcacc ggtgagctgc atctgttttc cgatgagcga catcttcctt atgaacgacc
    18241 tccactctcg aaatccatgt tgctggaaga ttccccgcag ttacagcagg tgttacccgc
    18301 taactggtgg caggaaaaca atgttcatct gcattccggt gtaaccatca aaacgctggg
    18361 tcgcgacaca cgagagttag tgttaaccaa cggcgaaagc tggcactggg atcagctttt
    18421 tatagcaacc ggcgcggcag ctcgaccgct gccgttgctt gatgcactgg gagaacgctg
    18481 ctttacccta caccatgccg gtgatgccgc cagactgcga gaagttctgc agcccgaacg
    18541 gtcagtcgtg attgtcggtg ccggaactat tggtctggaa ctggctgcca gcgccacgca
    18601 gcgcagatgt aaggtgacag tgattgaact ggcggcaacc gtcatgggcc gtaatgcacc
    18661 accgcccgtg caacgctatc ttttacagcg ccatcagcag gctggtgtgc gcattctgct
    18721 caataatgcc attgaacatg tggtcgatgg tgaaaaagta gaactgacgc tgcaaagtgg
    18781 ggaaacgctt caggctgatg tggtgattta cggtattggt atcagcgcca acgagcaact
    18841 ggctcgcgag gccaaccttg atactgccaa tggcattgtc attgatgagg cttgccgcac
    18901 ctgcgatccc gcgatctttg ccggtggcga tgtggcaatc actcgtcttg ataatggtgc
    18961 actacaccgc tgcgaaagct gggaaaacgc caataaccag gcgcaaattg ccgctgccgc
    19021 aatgttaggg ctaccgctac cgctactgcc gccgccgtgg ttctggagcg atcagtacag
    19081 tgataactta cagtttattg gcgatatgcg tggcgatgac tggctttgtc gtggcaaccc
    19141 ggaaactcag aaggcgattt ggtttaatct gcaaaacggc gtgcttatcg gtgcggtcac
    19201 gctgaatcag ggccgtgaga ttcgcccaat tcgcaaatgg atccagagcg gcaaaacgtt
    19261 tgatgcgaaa ctgctgatag atgagaacat cgcgcttaaa tcactgtaat caggataatt
    19321 agcgaatatc tcaatgcctg gggcgtggcg aggtgcaaga gtgtgtatta cgtttaaatc
    19381 acattatctt gcaaagggat tggttatgaa cacactacgt tattttgatt ttggagctgc
    19441 ccgccccgtt ttgttattaa ttgcccgtat cgccgtggtc ttaattttca ttatttttgg
    19501 ttttcccaaa atgatgggct ttgacggtac ggtccaatat atggcctcgt tgggcgcgcc
    19561 aatgccgatg ctggcagcga ttattgcggt agttatggaa gtgcccgccg cgatattaat
    19621 cgtgcttggc tttttcaccc gtccgctggc ggtgctgttt attttctaca cgctgggtac
    19681 ggcggtgatt ggtcaccatt actgggatat gaccggcgat gcggttgggc caaatatgat
    19741 taatttctgg aagaatgtca gtatcgctgg cgcgttcttg ctattggcaa ttaccggacc
    19801 gggggcaatt tctctcgatc ggcgttagga aagatgccgg atgcggcgtg aacgccttat
    19861 ccggcatttt ataaattaca gccactctac ccgcaacgac atctctgagg ttgttgattc
    19921 ccctggtgct agcgcaatga gatcaccccc ttccgggcga tgatgctcat ccggcgcatg
    19981 gctcatcggt tccagacaga agaaatcaaa cgcatatcct ttatcaaacg cagggtcgga
    20041 aacaaagatg aaataacacg gtgcaggtgg cgtcgtttcc atgatgatgg cataaccttc
    20101 ctgcggctgc tcgatgcggg cctgaccatt ccatccggca aaaccattgt tcacccactg
    20161 acgcggcaac ggcgcgagct ggttaaaatc cagttcctgc ggtagctgct cgcaaaactc
    20221 acccgccagc cactgctccc gttccagcca gtaaccgctc gcctgcgcct gaatccgcgt
    20281 ttgtggtgac aacggaaaat aaggatgcca gccggtacca aatggcagcg tctctgcccc
    20341 ttgattggtg acagagagcg tcaccgtcag cgtatccgcc gttaaatgaa acgcctgact
    20401 tacccgatag tgatagacac cgctgcgatg ttcatacacc agacacaaac tatcatcgct
    20461 gtgcgagaca cattgccact cgcccagcca gccatcgccg tgtagatagt gcgcatccca
    20521 ctcaacattc ggttgcagtt gatactcacg cccctgccag acaaaacggt tgccgctcac
    20581 ccggtttgca aatggcacca gcggaaaaca tgaggcatcg gttgccacac cgcttttttt
    20641 accaggacgt aagagcggcg tcgtatcgcg ccagaagcct tcgattacgc cgccctggtc
    20701 agaaacgtcc agctttagcg acccgtggga taaggtatag atggtcatcc gctaactcct
    20761 taatccggga agttaatcac aactttcccg catttgccgc tcgccatcag cacataggca
    20821 tcacctgcct gttctagcga gaagcgatgg gtaatggcgt tacgcggcca cagcttccag
    20881 tcagtcaaat cgtgggcgca tttttccata tggaacagac tggtcaccca ggagccgata
    20941 atccgccgtt gatggtgcat cagatcagcg ctgacctcga attccacttt tccggtttca
    21001 ccaatgtaaa ccacccgccc ccagtcagca gtggattgca gtgccagcaa gcgacctgcg
    21061 gcattaccgg aacaatcgag cgcaacatcc gcgccaccgt gggtaagttc agcaattatc
    21121 tgcggcagac cttcggtggt tgctaaatag ccgtgatcca tcccccctaa ctgttttgcc
    21181 atcgccagac gttccggcag catatcaacg ccgatgatcc gttttgcacc gcgacctttc
    21241 gccagcatca tcgccatcat gccgaccggc cccagaccga ccaccagcac gttatcgctg
    21301 ccggaaactt cgccgcgcaa aatcccttca taggccgtgc caacgccgca actgataaac
    21361 gcaccatctt cgtagctcag cgcatccggc aggaggatca gatctttttc ttccgccagc
    21421 agatattcgg catgaccgcc gtcacgctgc cagccgtaag ccgcttttcc tttgccagta
    21481 caagagatag gaaagccgcg gcggcagttc gggcaaaaac cacagccaga aatgtgatac
    21541 accagcacgc ggtcgccctc tttaaaatgg cggcagcctt gccccatcgc cacaatctgc
    21601 ccgcacggtt catgaccgtt gataaatccc tggtataacg gtttatcggg tgccgccgcc
    21661 gtggcacggt gttgatgata gatatagtgg acatcgcttc cgcaaatccc ggaggatttc
    21721 attttgatca gtacctggtt aatccccggc gtcggcaccg caacttcccg cagatcgacg
    21781 gttgaatttc ctagtaaata agcagccagc atcgttttca taaatccctc attaacaata
    21841 cgattaattt tcatccctgc agcacccgcg ccaggggcgt taaggtttag cgtttcgctt
    21901 tactgcttcg ctgggtcagc aagatattcg ccagcaccgc caccacgatg atgacgccgc
    21961 gtaccacctg ctggaaaaag gagttaatac cgagcagcac cagaccgtta ccgattagcg
    22021 taatcaccag cacaccaagc aatgtaccga acagggaacc gcgaccgccg gaaagtgccg
    22081 taccgccgac cacgaccgcg gcgatgacgt caaactccag accgtttgcg gcacctgcgt
    22141 taccggaacc gaggcgtgcc gccaacaaaa tgccggtcac cgccgctaat aatcccgaaa
    22201 gggtaaagat aagaatgcgc actcgacgaa cgttaatacc gcacaactgc gccgccgtgg
    22261 cattaccgcc aacggcaaaa accgagcgcc cgaaggcggt ttttcggcta atgaacacaa
    22321 acagcgcaaa caacaccatc atgatcagcg cggatactgg cacaccgaga aattgtccgc
    22381 ccagccagtc cagcacctcg ttttcgtcaa tcggcactgg cagtgcgttg gtcataaaca
    22441 gccccattcc gcgcagagcg ctccacagcc ccagtgtggc aacgaaactt ggtacgttaa
    22501 acacgccgcg cagcaccccg gcgagcgttc ccatcagcgc gcctaacagc aacaccagca
    22561 gacaagcaat cgccaacgga acttcaaatt gcagcaaaaa tgccaggcac accgagacaa
    22621 aagccaccat cggcccaacg ctgacatcaa tttcaccgga gataataatc agcgtcatcg
    22681 cccaggcggc aatcccaatg gtggcggcat cgcgcagcac gttcatctgg ttattcaatg
    22741 agataaagcc aggcgcgttc agggagaaga ccagataaag aatggcaatc accaccagca
    22801 aaccgatctc attaatatgg cgactgacaa attgtttgag cgagacgctc ttgccctgcg
    22861 gcaatggtaa tgacgaagca tacatcttca ggttcctcgg taaaattcat cagtgcacag
    22921 acagaatggc ggacatcagc tcatccacat tgaccggcga gtgaaactcc tgcgagaacg
    22981 tgccgtgctg taataacagg atgcggtcac acaccagcgg caattcctcc acttcactgg
    23041 agataaacac cacgcttttt ccttcggcag ccagctcacg gacaatacgg taaatctgct
    23101 gtttggcttc gatatcgacg ccgcgcgttg gctcgtcgag caacaaaatc tggctggcgg
    23161 catagaccca acgaccgatc accacttttt gctgattgcc accagaaagc gtgccgatgg
    23221 gtgtttcgct actggcagcc ttgaccgtca tccgctgcat cacctcttcg gtcaggcggc
    23281 ggatggtgga ccattgcagc acaccgttgg tgctgatttt ttgccgattg gtcagcactg
    23341 tattttcgtc aacgcctagc cagggaatga tccccgcttc tttgcggttt tctggcgtat
    23401 agccaatgcc gcgtttcagc atgtcgccgt aatcggggcg catgattttc tcgccgttga
    23461 taacaatttc gccctgttca tacgtctcca gcccaacaat cgccttcagc aattcactgc
    23521 gccctgcccc cagcaggcca gcaatgccaa gcacttcgcc acgacgtagc gtaaagctga
    23581 tatcctccag cttgggctta tggcgtaacg cacggacttc cagtacggcc tgatccacaa
    23641 tttcctgagg ggcaaccggc gcaatatcaa cgtgatcacg ccctagcatt agcgacacaa
    23701 tatgatgcgt ggaggtgttt tcgagcatca catcgcccgc cacctgacca tcgcgcataa
    23761 cggtggcata ggaggcaatg cggcggattt cttccatccg gtggctgaca taaatcaccg
    23821 ccacgcccag tgccgacatc tttttcaccg cactgatcac cagttcaact tctgcactgg
    23881 caagcgagct ggtgggttca tcaagaatga ccacgcgcgg ctcgcccttc atcacccgtg
    23941 caatttccac cagttgcttt tgcgccgggc ttagcgttga aacaagttgt tcaggactaa
    24001 cgtcaacgcc cagcgcctgt aagcaacgtt gggcatcctg cgccatttgc aggtaatcaa
    24061 tcatgccgtt gcggcgaggc cattgaccaa ggcagaggtt ttctgccacc gtcagccctt
    24121 ccaccagact taattcctga taaaccgcac gaacccccag ttcagcggca cggcgagtca
    24181 acgtagcttc gtcaccttcc agtcgcgtct cgccaatcca gatatcaccg ctatccgggc
    24241 gttcgctgcc ggtaagcatt cgaatgagag tcgatttgcc cgcgccgttt ttacctaaca
    24301 gcgcacgaac ttcgccttta ttgagcgtga agttaacgtt atccagcgca acgacgccgg
    24361 ggtaacgctt atttcctgcc accacttttg ctaccgggac tgcctctgtt gccgtgaaca
    24421 tagggacctc tgcgaatcag cgattagggc agaccatcaa cgtgcgttgc cagccactgt
    24481 ttaccatctt ccgttttggt atagagatcg attggcacct gaatcacttt ttcaccgtcg
    24541 gcttgtttat tgataacctt caatgtttgc gcgaaaacag cattgcccat tttcttaccg
    24601 gaaatatcca ctaccgcttt cagcacctga ttgttttcca gctcctgagc aatttcggtt
    24661 gtcatatccg aaccgaaaac agcaattttt ccggcctgat tttgattacg taccgctttt
    24721 accgcgccga gtgtcgcacc gcccgactcc cccataatgg cgttgagatc cggcgtggag
    24781 ataatcagtt tttcaccaac ggaaatcgct ttatctaaaa cagtcccttc ctggttagcg
    24841 acaatttgcg cgccgggaac gcgggatttt aatacttctt caaatccttt acgtcgctgc
    24901 acacaaactt caaaggcttc gcaattgatg acggcaattt tcggctggtc aattttattg
    24961 gcaataaaat aatcggcggc agcgttaccc agtttttacc aaattccagt ggatcgccga
    25021 ccagatacgc cgagacatat ttatcgacac ccttttgatt aatacaggtg ttgtagcaaa
    25081 tcaccggaat acccgcttca ctggcgcgac gaacggtacg gctactgcca ttttcagaca
    25141 ctgccgataa aataatggca tcgacattac gcgccacgag ggtatcaaca aaggtacttt
    25201 ctttcgaaat atccccctgg gcgttagttt caattaactg tacctgtact gaagaatctt
    25261 ttgccgcatc ctgaacgccc tgacgcactc cagcgtaata tccctgggta tcaaggtata
    25321 ttgcaccaat ggtcatttct ttatccgcag ccctggcaaa tagtgcgcta cctaatagcg
    25381 tagccattaa taatagatta cgggttgttc tcattttttt aggcatagtg cctcctgtag
    25441 ggtttttatt aacaacggct tattctaatt attttgtgat gagcggcagc gcgtgccgct
    25501 caaatattac agttaatagg gtttagtgaa cgtagttaaa aataaaaggc atcacagtgg
    25561 tgaataatgc cgctttcggc caggccggat taatcgccgt caattcagcg cgagcagact
    25621 catattgcgc aacctccccg agtcccgcgg acgccagcat cgccaccatc aggcattttt
    25681 ctttatgctg ctgttgtaaa tcgccataca gcgataacag gtcaggttgt gaaacagcaa
    25741 agaaatccgc ctcaatactg gttttcgcca tctcttgcgc ccactgtttc atttcactaa
    25801 acagttgctg tgcggtttgc tgttcaccca gcagtcgcag cgccattcct tgccagaaga
    25861 gataatcaac cggctgatcg ttgtaataac tgtggatgtt aatggtgcga tcgccggtcg
    25921 ccgccagacg taaacaacgc atcgcttcag tttcatcgcc ctgcgcgttg gcgcatatcg
    25981 cctgccagaa ccagatgtcg ttatcagttt gccccggtaa acggccttcg cttaaattct
    26041 ccggataata cagcgcggca tgaagcagtt cgctggcctg ctgcggctct ctggcatcaa
    26101 gatgctgcca ggcgcgtaat aactggttga ggataaactg actggtgacc ttcccttccc
    26161 cgccttccca cgggtggaat ttacgcgtgg cgagaatgtc cgccgctttg tctgcctgac
    26221 ccgtgagatg ccacaaattg agcagttctg cggtcatgtc gtcgcgtttc agcgcaattt
    26281 ccagattatt ttccagacgc gccagtcgtt tctccggtgt ggctccactt aacttatcca
    26341 gcaaatcccg ttcgaaaagc agacgtgcat cctgcggcgc aagctgataa gcattatcaa
    26401 gataacgcgc ggccagctca taatcgtgtt tcttattcca cgcatggatc gctaacccgc
    26461 gccagccgtc ggcaaactcc ggcgacatct ctacgcaacg ttgccataag gcaatggctt
    26521 tgttgtagct acgtttgttg tagtagaagc aggccagtaa atggcgagca aaccaacact
    26581 cttcaatact ctccagcgcc gccacttctt ccagcgtatt cgggaaacgg acaaactgcg
    26641 ggaagacatc aatggctttt gcgaccagtt cgccacgttc ggctttcggc agcaagctgg
    26701 cttgcaggta aagcggcagc gtgcgctggc agtccagtgc gttcagcatc tctgccgcca
    26761 gggtgggcat tccccagtta atcagttgcc cggcggtcat cagagcgtta acgtcgcgtc
    26821 cctgacacag accgcgccac tgcgcgaggg ctgattcgct acgaccatcg aaccagttca
    26881 gccaccacag agtggcgttc agcggataat cacgcagcag tttctcgcgc tgcaaacgcg
    26941 cgttgtcctg acgaccactt aacaccagca gcagattatg caagcaaagc acttcctgat
    27001 tggttgggca ggcacgaaga ctttgttggc aaaaatccag acccgcgtcg aagttatcat
    27061 tacgcgccgc cagtcgtgcc agaccataat agccgcctgc tttactattg ccgctccaga
    27121 ccgcacgcca gaaatcctct tcggcttgtt gatattgtcc ctgacgttcg taagcactgg
    27181 cgcgaatcaa acttgcttgt ccacactgcg gttttttgtt cagcgcatgt gcacgtttca
    27241 gcgcctgact ggcatacgcc accgcttgcg ggaaatctgc gcggttatat tccagcatcg
    27301 ccagcgccag gttacagcga taatccagcg ggtccagcgc cacgccgcgc aggtagtaat
    27361 cgaacggtga acggctggcg tgatgatatt gctccagatg ctgaccgata aaccaggctt
    27421 catctgtact ggtaatgtct tgtgctgcca gtggcgcttt ggcgacgtcc ggcaacggca
    27481 actcctgcgg ctgatgttcc tgataactca gtacaatatt gccgtcggca tcggagagtt
    27541 caatggtcag tctgtcagga ttgataccgt gcaacacgcc ctggatggcg gtcgctggtg
    27601 tcagggccac ggcatcatcg agcaacgcgt tgcatttgcc gatttcgcgg atcgccagac
    27661 ggtatccgtt caacggagag atggcataca gcccccactc aatcccccgc tcactacgct
    27721 ggagttttat caccgcatcg cgggaggcat tttgcaccat gcccagagaa tgataaggca
    27781 ggaaatactg ctcgaaacgc ttctcttcgt aagcatcaag ccaggtaaaa tcaggctggt
    27841 tatcggcaaa aataccggtc atcagttcga tatacgggcc gttattgtcg gtcagactct
    27901 tatcccacgc ctggccaaat tcactgtgtc cccaactcca ctgtttttta cctggcgcaa
    27961 tatggtggtt ggcaacgtgc agcaaaccgc catcttcatc gtgacaccac gcgccgacaa
    28021 aatcgtactg cgatttttcg gccatatatg aggttggaac aggcacattt ttatagcgag
    28081 aaatgtccac tccggcggag tagtccactt tgtagtaagt gccggtggcg atggggaaag
    28141 cggagacggc ccgtttgccg tgatcaaaca ccgcagtcac atccggtggg aaaacgctct
    28201 gatgcccttc accccctttc actgccgggt tgacccacca caagaaatga cgcggcgtgg
    28261 cgttgccgtt atagacgcgg ctggcgattt ccagtgccgc ccggtcaggg cgcagggtga
    28321 aacctgtcat cacctgtaaa ccatgcatcg gctccgtttc gccgacccac accgtctgtg
    28381 caccgtcttc atgggcttcg agggtgaaat caacgggcat aaaggtggtc gggcgatggt
    28441 gttgcggcca gttaaactca atcccgccgg agatccacgg tcccagcatc cccaccagcg
    28501 caggtttaat gacttcattg tgatagacaa aatcgcgctg tttcacttta tcccatgcgc
    28561 gatgcacccg accgcccagt tccggcagga tcatcacttt gatgtagtcg ttttccagcc
    28621 acaccgcctg ctaggatttc agggttttct gctcgctcag cgtatcggtc acgccgtagg
    28681 gataaaccgc gccggacgat ccctgataaa cgcgattttc caggaacatg ggatgtatat
    28741 cctgcggccc ggtttcatag gtcgggatct caacgcgctc ttgccacact tttactggag
    28801 tcatggtgcc ctcaatatta acaagacata ctgaattaaa agatttgtgg cagtgtattg
    28861 aacaatctgg caatgttttc gcggaataat cacgcaatta actaaacaag gtttagtgaa
    28921 gatgagagcc tgcattaaca atcaacagat tcgccaccat aacaaatgcg tgattctgga
    28981 actgctgtac cggcaaaagc gcgccaataa atcaacgctg gcccggctgg cgcaaatttc
    29041 gattccggca gtcagtaata ttttgcagga actggaaagc gaaaaacggg tggtgaatat
    29101 tgacgatgaa agccagacgc gcgggcatag tagcggtaca tggctgattg cgccggaagg
    29161 tgactggacg ctgtgcctga acgtgacgcc caccagtatt gagtgtcagg ttgctaatgc
    29221 ttgtttaagt ccgaaaggcg aatttgagta tttacagatt gatgtaccga cgccgcaggc
    29281 gctgctgtcc gaaatcgaaa aatgctggca tcgccaccgt aaattgtggc cggaccgcac
    29341 catcaatctg gcgctggcaa tccacgggca ggttgatccg gtgaccggcg tatcgcaaac
    29401 catgccgcaa gcgccgtgga caacgccagt tgaggtgaag tatctgctgg aagagaagct
    29461 gggcattcgg gtgctggtcg ataatgactg cgtgatgctg gcgctggcgg agaaatggca
    29521 aaataattcg caggaacggg atttctgcgt gatcaacgtt gattacggca ttggctcgtc
    29581 gttcgtgatt aacgagcaaa tttatcgcgg cagtttgtat ggtagcggac agattggtca
    29641 caccatcgtt aatccggatg gagtcgtctg cgactgtgga cgttacggca gcctggaaac
    29701 cgtcgcctcg ttaagtgcat taaaaaaaca ggcgcgggta tggctaaaat cacaaccggt
    29761 taatactcag cttgagcctg aaaaactgac aaccgcgcag ttaatcgctg cctggcagag
    29821 tggagaaccg tggatcacca gctgggttga tcgctctgcc aatgccattg gtttgagtct
    29881 gtataacttc ctcaacatcc tcaatattaa tcagatttgg ttgtacggtc gcagttgtgc
    29941 ctttggtgag aactggctta atactattat tcgccagaca ggatttaacc cgttcgaccg
    30001 cgacgaagga ccgagcgtga aagcgacgca aattggcttc gggcaattaa gccgcgcaca
    30061 acaggtgctg ggaattggct atttgtatgt tgaggcgcag ttacgacaga tttgatggcg
    30121 cgataacgta gaaaggcttc ccgaaggaag ccttgatgat cataaacgaa aaattgcctg
    30181 atgcgctacg cttatcaggc ctacacggag attgcaatat attgaatttg caagattttg
    30241 taggcctgag aaggtgttca tgccgcatcc aacatgaaca acgagcacat tgtcagcaaa
    30301 tcaccgtttc gcttatgcgt aaaccgggta acgtgcgcag atgtcgagaa ctttaccttt
    30361 gatgcgctcg ataacggctt catcattgat gctgtccagc acgtcacaca tccagccagc
    30421 cagttctttc gcttcggctt ctttaaagcc gcgacgggta atcgccggag tacctacacg
    30481 aataccggag gtcacaaacg ggctcttcgg atcgttcggt acgctgtttt tgttgacggt
    30541 gatgttagca cggcccagag cggcgtctgc ttctttaccg gtcaggtttt tatcaaccag
    30601 atcaaccagg aacaggtggt tatcagtgcc gccggaaacc actttgtagc cgcgctcgag
    30661 gaacacttct accatcgctt tagcgttttt cgcgacctgc tgctggtaag ttttgaactc
    30721 aggctccatc gcttctttca gagcaaccgc tttaccggcg attacgtgca tcaacggacc
    30781 gccctgacca ccagggaaaa cggcagagtt cagttttttg tacagctctt cgctaccacc
    30841 tttcgccaag atcaggccgc cgcgcggacc cgccagggtt ttgtgagtgg tggtagtaac
    30901 aacgtgagca tgaggaaccg ggttcgggta gacgccagca gcaaccaggc ccgcaacgtg
    30961 cgccatatca acgaacagat aagcaccgat gctgtcagcg atttcacgca ttttcgccca
    31021 gtcaaccacg ccggaatatg cagagaaacc accgataatc attttcggct tgtgttcttt
    31081 ggcttgtttt tccagatcgg cgtaatcgat atgaccggta gcatcgatac cgtaaggaac
    31141 gatgttgtac agtttaccgg agaagttaac cggagaaccg tgagtcaggt gaccgccatg
    31201 cgccaggttc atacccagaa cggtatcacc tggttccagc agcgcggtgt agaccgcaaa
    31261 gttagcctgg gagccggagt gcggctggac gttagcgtag tcagcgccga acagttcttt
    31321 cgcacgatcg atcgccagtt gttcaacgat atcaacatac tcgcaaccgc cgtagtagcg
    31381 tttgcccgga taaccttcag catatttgtt ggtcagctga gaaccctgcg cctgcattac
    31441 gcgcgggctg gtgtagtttt cggaggcgat cagttcgatg tgctcttcct gacgtacttt
    31501 ttcctgctcc atagcctgcc acagttcggc atcataatcg gcaatgttca tttcacgctt
    31561 taacatccgc atctcctgac tcagctaaca ataaaatttt tggcctttat aggcggtcct
    31621 gttggacaac ggcgaacagt ataaccgaat cattgtgcga taacaggtct tgacaaagga
    31681 atttacgcaa acgattacct tcaggctacg caaggctttg gagaataaag agcttgcaac
    31741 cggaaacgga tttcttttca ggtttgtgat gcaaattttt cacttcatca cattctttct
    31801 gaaaaacacc aaagaaccat ttacattgca gggctatttt ttataagatg catttgagat
    31861 acatcaatta agatgcaaaa aaggaagacc atatgcttga cgctcaaacc atcgctacag
    31921 taaaagccac catcccttta ctggtggaaa cggggccaaa gttaaccgcc catttctacg
    31981 accgtatgtt tactcataac ccagaactca aagaaatttt taacatgagt aaccagcgta
    32041 atggcgatca acgtgaagcc ctgtttaacg ctattgccgc ctacgccagt aatattgaaa
    32101 acctgcctgc gctgctgcca gcggtagaaa aaatcgcgca gaagcacacc agcttccaga
    32161 tcaaaccgga acagtacaac atcgtcggtg aacacctgtt ggcaacgctg gacgaaatgt
    32221 tcagcccggg gcaggaagtg ctggacgcgt ggggtaaagc ctatggtgta ctggctaatg
    32281 tatttatcaa tcgcgaggcg gaaatctata acgaaaacgc cagcaaagcc ggtggttggg
    32341 aaggtactcg cgatttccgc attgtggcta aaacaccgcg cagcgcgctt atcaccagct
    32401 tcgaactgga gccggtcgac ggtggcgcag tggcagaata ccgtccgggg caatatctcg
    32461 gcgtctggct gaagccggaa ggtttcccgc atcaggaaat tcgtcagtac tctttgactc
    32521 gcaaaccgga tggcaaaggc tatcgtatcg cggtgaaacg cgaagagggt gggcaggtat
    32581 ccaactggtt gcacaatcac gccaatgttg gcgatgtcgt gaaactggtc gctccggcag
    32641 gtgatttctt tatggctgtc gcagatgaca caccagtgac gttaatctct accggtgttg
    32701 gtcaaacgcc aatgctggca atgctcgaca cgctggcaaa agcaggccac acagcacaag
    32761 tgaactggtt ccatgcggca gaaaatggcg aagttcacgc ctttgctgat gaagttaagg
    32821 aactggggca gtcactgccg cgctttaccg cgcacacctg gtatcgtcag ccgagcgaag
    32881 ccgatcgcgc taaaggtcag tttgatagcg aaggtctgat ggatttgagc aaactggaag
    32941 gtgcgttcag cgatccgaca atgcagttct atctctgcgg cccggttggc ttcatgcagt
    33001 ttaccgcgaa acagttagtg gatctgggcg tgaagcagga aaacattcat tacgaatgct
    33061 ttggcccgca taaggtgctg taatttgatg ttgccggatg gaaacatccg gcaacccttg
    33121 acgcggttta aattgccgcg tcgtcctctt caccggtacg gatgcgaatg acccgagcca
    33181 cgtcaaagac gaagatttta ccgtcaccga ttttgccggt ttgcgccgtg cgaataatgg
    33241 tatcgacaca ggtatcgaca atgtcgtccg gtacgacaat ctcaattttc actttcggca
    33301 gaaaatccac catatactcc gcgccgcggt acagctcggt atggcctttc tggcgaccaa
    33361 agcctttcac ttcggtcacc gtcatgccgg taataccgac ttcggccagt gcttcgcgga
    33421 catcgtccag cttgaagggt tttataatcg catcaatctt tttcatgcta ttccttgaaa
    33481 aggttgcctg tcttttgatc tgctaaacgt aacacataac gccaattcat tccttgaaat
    33541 cgtttgcatc cagctcgtgt cgggaaagca gtttataaaa ttctgtccgg ttgcgccccg
    33601 ccattctcgc cgcgtgggtg acgttgcctt tggtgatttg cagcagctta cgcaagtagt
    33661 tgagttcaaa atgattacgt gcctcaacaa aggttggcag cgccgtattt tcaccctcca
    33721 gcgcctgctc caccagcgca tcactaatca ccggagatga ggtcagcgcc acgcactgtt
    33781 caatcacgtt gaccaactgg cgcacattac ccggccagct cgcggtcatc aggcgtttca
    33841 tcgcatcggt agagaacgcg cggacaaacg gtttatgtcg ctctgccgcc tggcgcaaca
    33901 ggtgatttgc cagtagcgga atgtcttctg tgcgctccgc cagtgccgga attttcaggc
    33961 tgacaacgtt gaggcggtaa tagagatctt cgcggaattc cccgcgcgcc atcgcttttg
    34021 acagatcacg gtgagtggca gaaataatcc gcacattgat atcaatatcg cggttactgc
    34081 ccagcgggcg cactttacgc tcctgcaaca cgcgcagcag tttgacctgt aacggcgcag
    34141 gcatatcgcc aatttcatcg agaaatagcg ttccgccttc cgccgcctgg aataaacctt
    34201 cgcgattgct gacagcgcca gtaaacgcgc cacgcgcatg accaaacagc tccgactcca
    34261 gcaattgctc gggcaatgcg ccacagttaa tagcaataaa tggtttgctg ttgcgcgggc
    34321 tggcgttgtg gatagcctgg gcgaaaatct ctttcccggt gccgctctga ccgttaatca
    34381 aaacgctgac gtctgattgc gccaccagcc gcgcctgttc cagcaaacgc agcatcagcg
    34441 ggctgcgggt gacaattgcc tcgcgccagc gttcatcggt ggctggcgcg gattgctcca
    34501 gcgcatcgtc aattgcctga tatagcgcat ctttatcgac aggcttggtg aggaaactaa
    34561 aaacgccctg ctgtgttgca gcaacggcat cggggataga accatgcgca gtaagaataa
    34621 ttactggcat tcccggctgc actttctgga tttcagcaaa tagctgcata ccgtccattt
    34681 catccatccg caggtcgctg ataactaaat ctactttttc gcgattcagt acccgtaatc
    34741 cttcagcgcc actttccgcc gtgaccacac tgtagccttc gctggtcagg cgcaggccaa
    34801 gcagtttcag caatcccgga tcgtcatcga ccaataataa atgcgcaggt ttatggctca
    34861 tcaggagtga cctcatgggt ggatggcgcg ggcttttcac tctcgtgcgg cgtatccggc
    34921 gagaaatttc cagccggttt gcgggtcgag agctggcgtt caatatcggt caggttttcc
    34981 agcttgcggg tggtgagttc cagttgctgc tgtagaacgt gatgttgctg gcgcaatgta
    35041 tccagctcgc tgtcgctgga ctgctggagt ttactgtaac gttggcgctc ttccgccagt
    35101 tgcagttgca gcgcctgacc gtcgcgccag agttgataca gtgggcgaac ctgtgccggg
    35161 atctcggtac ttaacgcttc aatacgcgcg accagctggc ggcgctcata cggcgtaatt
    35221 ttggcgtcgg cgagcaaaat cccttgttta aaggtatttt gccagctgcc gtcgtcatat
    35281 tggcgagctt gctgacgcga ctgcgcaggc attaaacgat cagcacaatc catcgcccgc
    35341 agccagtaaa gcggattggt ttcggttgat ttaccttgca gcgcccagat gtcgctacat
    35401 tcagtagaaa gatagtcagc cagttgataa accggaattt tttcttctgc tggcgtatca
    35461 atggctggct tattgtgatt ctgcacgcaa cccagcaatg ccagacatgg cagccctgcc
    35521 agccacagcc gtcggggcaa taatcgttga aaaatgtgtc gcatattcac cagacttaaa
    35581 gcctatccca gtgggcgtaa ttgttgcaga cagtctggac atggacagcg cggagaaacc
    35641 ggggcgtaca tatcgtacgt gaagatttcg agcactaccc ggggccgaac tgacaaataa
    35701 aatagcctga taggataggc tgttagcatt atttcgtgtt tttcgacgac ggtaattcaa
    35761 tgcggaaaca aacgtcttgc ccgctctcgt cgaccagata cagttcccct tgcatacggc
    35821 gaatacaatc cctggcaatg cttaatccca gaccgctgcc cttcaccgcc ccttttcgct
    35881 ggtggcttcc ctgaaaaaag ggttcgaaga tcatggcgcg ttcctcttgc ggaatgggcg
    35941 tgcctgtatt gatgacatca atataaaccc gcgcaccatg taaactgctg cgaaggcaaa
    36001 tgttaccgga ttcagccccg tagtgcaccg cattggagta aagattatcc agtacgctca
    36061 tcagcagcat tggctccgcc aggcaagctg ttgctttgag atcgacgtcg gtatgcatca
    36121 ttttagcccg tgcgggcagg ctatgagcag aaaccactgt ctccaccagc ggtgctaatt
    36181 caacattctc cagttccacc gcactgtccg cctgtttacg gttgtaatca agcagttgtt
    36241 cgatcagttt ttgcaaattg cagctgctgc tatcaagaat gctcaccacc tctttttgct
    36301 ctggcgtaag cggcccgaca acctgatcag ccagtaattc agtgccctcg cgcatactcg
    36361 ccagtggcgt ttttaattca tgagataaat gtcttaaaaa ttgatggcgt tgggattcca
    36421 gccatgacag gcgctcactt aaccaaagaa tacgttgccc aaccgagcgt aactcgctcg
    36481 gtccacagaa cgaaacgcta ttgcccagag aacgcccttc ccccagacgg ttgatcatgc
    36541 gctcgatatt tttcaccggc ccgataatca tccgcgtgaa aagtagcacc atcaccagac
    36601 tcaccagaaa tagcaccagc gattgccaac caaaatattg cccacgttcg gcgatttcac
    36661 gctgaagttg ctgcccacga gagaacacca ctgtgcgcgt ggcctgcacc atttcggtat
    36721 tggcactggc aaaggcttca agacgcgcgg cggcggcagc atcgggacca ctgttgttac
    36781 actgaagttg ggccagattg ttcaaatcct gacgcaatgc ctggtaaagt ttatcgtctg
    36841 gcagtacgcc tgcgtgggca tcgagcattt cgctgtaacg cttgcgctgg ctttgataaa
    36901 ccttcgcaag cgtagggtcg tccagcacgc aatactgacg gtagctacgc tccatctcca
    36961 gcgccgcgtt ggtcatcgct tcactacgcc gggcatcaat aagcgtagtg cggttaacca
    37021 gcgccgcctg atcgctaagc gcattcaggc tttgccaggc ttgccatgcc agcaccaaca
    37081 ggggtagtag aattagcaaa aatgccagca ttaccagttg tcgtaatgag cggggaaaaa
    37141 cgggccagcg tttcaaggtg ttactctcgt cagacgcgaa tagcctgatg ctaaccgagg
    37201 ggaagttcag atacaacaaa gccgggaatt acccggcttt gttatggaat aaggcggtgc
    37261 ctaactcgac gtttcgcccg atggttgata tagctacgct gatatcagaa gctggacggc
    37321 aggcaccttg ttgtgcgtca ttcgaatttt atgtagcacg tcccgaaggg gctgacataa
    37381 gtcggtgaat gagccactgc ttactattat gcagtaactg tgccaataaa gaaaatagtt
    37441 tggtaacgta ctgattatac gttgctttga gggatttatg gcttcttcgc tatttgaatg
    37501 taaatcagcc actgtgtcgc taaaaagaga caacttaaga taaacttatt agattgcaat
    37561 aaaatcaatc atttaagtgt cgccagaaag cgacacggta aaccacccat tgtcgtgatt
    37621 tacagacaca aaaaagctcc cggagttggg agcttatgat agtggttggt gcttaatgcc
    37681 gcatccgggc tgcaaaacca atgaactgat gacttacccc aactgcttac gcgcattgcg
    37741 gaaaatgcgc atccatgggc catcctcgcc ccagttttcc ggatgccagg agttgctgac
    37801 agtacggaaa acacgttccg ggtgcggcat cataatggtg actcgaccac tttcagtcgt
    37861 aaccgccgta ataccgttcg gtgaaccgtt cgggttagcc gggtaggttt cagtgacttt
    37921 gccgaagtta tcgacatagc gcagtgccac cagccctttg ctttccagtg ccgccagatg
    37981 cgccgcatca cgcacttcca cgcgcccttc accatgagag acagcaatcg gcatttgcga
    38041 gcccaccatc ccctgcaaca gcagagacgg gctttgggtt acttcaacca ggctgaaacg
    38101 cgcttcaaag cgatcggagg tgttacgcac aaaacgtggc cacaactcac tccccgggat
    38161 cagttcacgc aggttagaca tcatctggca accgttacat acccccaggg ccagcgtttg
    38221 cggacgatgg aagaaggttg caaactcatc gcgtacacgg tcattgaaca ggatagactt
    38281 cgcccaacct tcaccggcac ccagcacatc accgtaggag aaacctccgc acgcgaccag
    38341 ggcgtgaaaa tcctccagtc ccgtacgtcc agtcagcagg tcgctcatat gcacgtcgat
    38401 agcatcaaag cccccacgtt ggaaagctgc cgccatttca acatgcgagt tcacgccctg
    38461 ctcacgcagt acggcaactt tcggacgtgc gccagtggca atatacggtg ctgccacatc
    38521 ttcgttgata tcgaacgaca gttttacatt caggcccgga tcggcgtcgt tagatttcgc
    38581 ctgatgctcc tgatcggcac actccgggtt gtcacgcagg cgctgcatct gccaggtagt
    38641 ttctgcccac cagacacgca acgtagtgcg gctttcgctg aatacagtct gcccgttggc
    38701 ggtaatcaca aaacggtcac cggaaaccgc ctgcccaaca taatggacgc aatccgcaag
    38761 cccatgctgt gccagtacgg actcgaccgc ttcacggtca gcggcacgaa cctgaatcac
    38821 cgcacccagt tcttcgttaa acaacgccgc caggcgatcg tcacccagag aggcgatatc
    38881 cgcgtcaatg ccacaatgac ctgcaaaggc catttccgcc agcgttacca gcaggcctcc
    38941 atcagagcgg tcgtgatacg ccagcagctt acgctgtgca accagcgcct gaatcgcgtc
    39001 atagaagcct ttcagttgga caacatcgcg tacatctgcc ggtttgtcgc caagctgacg
    39061 ataaacctgc gccagcgccg ttgcgcccag cgcgttattg cctttgccca aatcaatcag
    39121 cagcagtgcg ttatcttcgg tacaaagctg cggcgtgatg gtgtgacgta catcttccac
    39181 gcgggcaaat gcagaaatca ccagcgacag cggcgacgtc atttcgcgct cttcgttacc
    39241 ttcctgccag cgggttttca tcgacataga gtctttaccc accgggatcg tcaggcccag
    39301 cgccggacaa agttcttcac ccacggcttt aacggcttca tacaggcccg catcttcacc
    39361 agggtggcct gccgccgcca tccagttggc ggaaagttta atgcgtttga tatcgccaat
    39421 ctgtgttgcg gcaatattgg ttaacgcttc accgaccgcc agacgggcag aggcggcgaa
    39481 atccagcagc gcaaccggcg cacgctcgcc aatcgccatc gcttcaccgt agtagctgtc
    39541 gaggctggcg gtagtgaccg cgcagttagc gaccggcacc tgccacggcc ccaccatctg
    39601 atcgcgcgcc accatgccgg ttacgctgcg gtcaccgatg gtcaccagga aagttttctc
    39661 cgccacggtc ggcagatgca gtacgcgttt caccgcgtct gcaatggtga tcccttcacg
    39721 gaccagcgca tcgcccttcg ctttcagcgt ttgtacatcg cgggtcatct tcggcgtttt
    39781 accaagcagg acgtccagcg gcagatcgat cggctgatta tcaaaatgac gatcgtgcag
    39841 agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg tagggtgcgc gctcacgctt
    39901 acacagttcg tcaaacagcg gtaattgatc ggcagcaacc gccagcacgt agcgttcctg
    39961 ggattcgtta caccagattt ccagcgggct catgcccggt tcgtcgctta gaatatcgcg
    40021 cagttcaaat ttaccgccgc gtccgccgtc gctcaccagc tccggcatcg cgttagaaag
    40081 accgcccgca ccgacgtcgt ggataaacag aattgggtta gcatcaccaa gctgccagca
    40141 acggtcgatc acttcctggc aacgacgctc catctccggg ttgtcgcgct gtacggaagc
    40201 aaagtcgagg tcggcatcag actgaccaga cgccatggaa gacgccgcgc cgccgccaag
    40261 accgatgttc attgccgggc cgccgagaac gaccagcttc gcaccgacgt tgatctcgcc
    40321 tttttgtacg tgatcggcgc gaatgttgcc gatcccgccc gccagcatga tcggtttgtg
    40381 ataaccgcgc agctcttcgc cattgtggct gttcactttt tcttcataag tacggaagta
    40441 gccgttcagt gccggacgac caaattcgtt gttaaacgcc gcaccgccca gcgggccttc
    40501 ggtcatgatg tccagcgcgg taacaatgcg ctcaggctta ccgaaatctt cttcccacgg
    40561 ctgttcgaag ccaggaattc gcaggttgga tacggagaaa ccaaccaaac cagctttcgg
    40621 cttcgcgccg cgcccggtgg caccttcatc gcggatttca ccgccggaac cggtcgccgc
    40681 gcccggccac ggagaaatcg ccgtcgggtg gttgtgagtt tcgactttca tcaggatgtg
    40741 cgccggttcc tgatggaaat tgtagcggcc cgtttcgtgg tcagcaaagt agcggcccac
    40801 ttcagaacct tccattacgg cggcgttatc tttataagca gagagaacgt gatctggcgt
    40861 ggtttcgaaa gtatttttga tcatcctgaa cagcgatttc ggctgttgtt caccgtcgat
    40921 aatccagtcg gcgttaaaaa ttttgtggcg gcagtgctca gagttcgcct gagcgaacat
    40981 atacagctcg atgtcgttcg ggttacgacc aagctttgtg aaagcatcct gcagatagtc
    41041 aatttcatct tccgccagag ccaagccaag acgcaggtta gcgtcgatca gcgcctgacg
    41101 gccctgcccc agcaaatcaa cgcttgtgac cggagtcggt tgatggtggg caaacaactg
    41161 ctctgcatca tctaaatcaa aaaagaccgt ttccatcatg cggtcgtgca gttcagcggt
    41221 aacctgctgc cattgttcat tggtcagcgt accggcttct atatagtaag caacgccgcg
    41281 ctcaaggcgg tttacctgtt gtagcccgca gttatgggca atatcggtcg ctttcgaaga
    41341 ccagggagag atggtgccag gacgcggggt caccagcagg agtttgcctt gcggggcgtg
    41401 gctggcgagt gccggaccat atttcagcag gcgttcaagt tgtgcgtgct catcatcgtt
    41461 taacggcgca ttgaggtcag caaaatggac atactcggcg taaatattgt gaaccgggag
    41521 cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg aatgccgaca gtgcaggcga
    41581 accacgcaga atttccatca taagtctctc gtcttctaag ctttcggtgt acccaagggg
    41641 ggggaaacgg gcgtcattat aaagaatctg atgcgctgac gaaaccgttt gcgtggaaat
    41701 aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg ctgtaataag ttgccatctg
    41761 gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac ctttcactat tattacgttt
    41821 tttcaagctg ggacgcgcac gacacagaga attaactaat tgaaaaaatt aaagattaat
    41881 tatctgttca tcggcattct ggcactgctg ctcgcggtcg ctctctggcc atccattccc
    41941 tggtttggta aagccgacaa ccgtatcgcc gccattcaag cgcggggaga gttgcgtgtg
    42001 agcaccattc atactcccct gacttataac gaaatcaacg ggaaaccttt tggcctggat
    42061 tacgaactgg cgaaacagtt tgccgattac ctcggcgtaa aactgaaagt gaccgtgcgg
    42121 cagaatatca gccagctgtt tgacgacctt gataatggta acgccgacct gctggcggca
    42181 ggactggtct ataacagtga gcgggtaaaa aattatcagc ctggccctac ctattattcc
    42241 gtgtcacaac aactggttta taaagtgggt cagtatcgcc cacgtacgct gggcaacctg
    42301 acggcggagc aactcaccgt tgcaccgggt catgtggtag ttaacgatct ccagaccctg
    42361 aaagaaacaa aattcccgga attaagctgg aaggtagacg acaaaaaagg ctctgcggaa
    42421 ttaatggaag atgtcatcga aggaaaactc gattacacca ttgctgattc tgtcgccatc
    42481 agcctgtttc agcgcgttca cccggagctc gccgtagcgc tcgatatcac cgatgaacaa
    42541 ccggtgactt ggtttagccc gttagatggc gataataccc tttccgccgc cctgctcgac
    42601 ttcttcaacg aaatgaatga agacggtacg ctggcacgca ttgaagagaa atacctgggg
    42661 catggcgatg attttgatta cgtcgatacg cgcacatttt tacgcgccgt cgatgcggta
    42721 ctgccgcagt taaagcccct gtttgagaaa tacgccgaag aaattgactg gcgtttgctg
    42781 gccgctattg cttatcagga atcgcactgg gatgcacagg ccacttcacc gacgggtgtg
    42841 cgcggcatga tgatgttaac caaaaatacc gcgcaaagcc tcggcattac ggatcgtacc
    42901 gatgccgaac agagcatcag cggtggcgtg cgttatttgc aggatatgat gagtaaagtg
    42961 ccggaaagtg tgccggagaa cgaacggatc tggtttgccc tcgctgcgta caatatgggc
    43021 tatgcgcata tgctggatgc ccgcgccctg acggcaaaaa ccaaagggaa tcctgacagt
    43081 tgggctgacg taaaacagcg tctgccttta cttagccaga aaccctatta cagcaagctg
    43141 acttacggct acgctcgtgg gcatgaagcc tacgcttatg tcgaaaatat tcgtaagtat
    43201 cagattagcc tggtgggtta tctgcaagag aaagagaagc aggctacaga agcggcgatg
    43261 caactggcgc aggattatcc ggcggtatcg cctacggagt tgggcaaaga gaaatttcct
    43321 tttctctcgt ttctttccca gtcgtcatca aactatttga cccattctcc ctctctgctg
    43381 ttttccagga aagggagtga agagaaacaa aattaatccg tcgaggattg cgcttttttc
    43441 tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc cgcgcactca
    43501 tccgccagta ttccttccgt aatttccact cggtgattca tacccggatg atgcagcaca
    43561 tccattaaag atcccgcagc gccagttttc gcgtcacgcg caccaaagac cacgcgacca
    43621 atgcgactgt ggatcatcgc tccggcacac attacacatg gttcaagcgt gacatacaac
    43681 gtggcgtcga tcagacgata attttgcatc accagaccac cctgccgcag ggccatgatt
    43741 tctgcatgtg cggtgggatc atggcgacca atcgggcggt tccagccttc gccgattacc
    43801 cgattgttat gcactaatac cgcgccgacc ggcacttccc gctcatccca ggcacgtttc
    43861 gccagcgtca gcgcgtgacg catccaatat tcgtggctaa attcgacttc agacaaaaag
    43921 aaaactccgg ttataaaagc gcggcgcatt atacacggac gctatgcttt actctattcc
    43981 agttgctgga gttcaccgcg cggggttact cgccaacgat gctgacagaa ataaagcagc
    44041 ggattgtcct gattactgtc gctatagcca ctgtacagcc gcagcggcgt gccgattttg
    44101 cgctccagtt gtgcgacctt ttcatgtccc agacaacgca tcgtcaatac ccaaccacca
    44161 tagccacgct gaatttggct ggcgataaga ttaacccgcg gcagccaggg cgtatcgaaa
    44221 taaaccgctt caaccagcgg ctgcggagag ccggtaatca accagatatc agcatcggaa
    44281 cttaacaggt aggtggttaa tcgctcctga accagcggaa aggcggtaac attgtcgcga
    44341 aaccagcgca cgaaatcggc ctgcaacgtc tgtaaacgtg cttcgctgtg accaaaagtg
    44401 cacccccaca gaagcagact catcggccag cgtgccgcac gaccttttat caataacgca
    44461 atggctataa tcggtaacaa cggcaggaca agtaacgcat tcagcggttg gcgacgcagt
    44521 aaatagcgca gaaaactgcc gaacatatcc tgctgatgca atgttccatc taagtcaaaa
    44581 aacaccacac gacgctcgtg agttgccaaa ccatactcct ctggactgaa acatcctgat
    44641 taattttcgc tactacatag cctaacagat agatcatcac ttttccggca acaatcgacc
    44701 gcatcatggc taactggaat ttttaattca tggcaattag cggcaatgga atataaaatt
    44761 cactcgcgtg tgtctcatat tgcctgttgt cgccactatt tcatcgcgag gaaagatatg
    44821 aacggcttac ttcgtatccg tcagcgttac caagggcttg cccagagtga taaaaaactg
    44881 gcggattatc tgctgctaca acctgatacg gcgcgccatt taagctctca gcaactggcc
    44941 aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact cggctataaa
    45001 ggttttccgg cgcttaagtt ggcgttgagt gaagcgctgg caagccagcc ggaatcgccc
    45061 tccgtgccca ttcataacca aatccgcggt gatgatccgt tacggctggt cggcgaaaaa
    45121 ctgattaaag aaaataccgc cgctatgtac gcaacgctaa acgttaatag tgaagagaaa
    45181 ctgcatgaat gcgtaacaat gttgcgctct gcgcggcgga taattctgac cggtattggc
    45241 gcttcgggtc tggtggcgca aaactttgcc tggaagctga tgaagattgg cttcaatgct
    45301 gccgcactgc gcgatatgca tgcgctactc gcaacagtac aggcgtcgtc ccctgacgat
    45361 ctgttattag ccatttccta caccggtgta cgacgcgagt taaacctggc ggcagatgag
    45421 atgctgcgag tgggcggaaa agtgctggcg attaccggct ttactccgaa tgccctgcaa
    45481 cagcgtgctt ctcattgcct gtataccatt gccgaagaac aagcgacaaa cagtgcttca
    45541 atctctgctt gtcacgctca gggaatgtta acggatttgc tgttcattgc gctgattcag
    45601 caggatctgg aactggcacc agaacgtatt cgtcatagtg aagcgctggt gaaaaaactg
    45661 gtctgagtaa agaatgcgcg tataatgccc gcccggtttg tgttgttttg agagtttcct
    45721 tatggcgctg ttaatcacta aaaaatgcat caattgtgat atgtgtgaaa tcaaataaaa
    45781 acacatttat tttcatagtg atacagcaat gtgttattga atgtgttgtt ggatttatgg
    45841 tgcaaaaaaa ttttgcataa gttcagcaac acaaagaaca ccactttctt taacgtcctc
    45901 cgcgccctaa aggacggggc tttacggcgc aaaaggcctg tgacatacaa cattaacttt
    45961 tcagtcaacc gtagcacgtt cttgtatacg ccgtgtctgc ggttttatat aaccctggct
    46021 ggaaacctct tatacaaagt cgattcgcca acatcataga tgatcgccac cttctggcga
    46081 gaaactcctg atgcaattag tcgcccggcc tgcgcccatt attctggtga aagtttggga
    46141 cggcgaccgc caattcgtcc ctgtgcgcga gcagcttcca gtcctgcttt tgttcgttca
    46201 acaatcagtt cacgttccat ttcagccagg gcacccatca catggaagaa aaagcgtccc
    46261 attggtgtgc tggtatcaat tgaatcagtc agactacgaa agttgatgcc tcgttcgcgc
    46321 aacgcctcca ccagcacgac aagatgccgc atactgcgcc ccagccgatc cagcttccag
    46381 acaaccagtg tgtcgcctgc cgataatgtc ttgagcagtt ttttcagccc cggtctttcg
    46441 gactttgtac cacttatctt gtcttcaaaa atcagctcgc atcctgcaca gttcaacgca
    46501 ttacgttgta gatcggtatt ctggtc
//
LOCUS       DAZOYF010000012        45326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZOYF010000012 DAZOYF010000000
VERSION     DAZOYF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.012"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            185..484
                     /locus_tag="ACHUOF_000685"
     CDS             185..484
                     /locus_tag="ACHUOF_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR0609989.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            511..939
                     /gene="uspD"
                     /locus_tag="ACHUOF_000686"
     CDS             511..939
                     /gene="uspD"
                     /locus_tag="ACHUOF_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR0609990.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(944..1690)
                     /gene="fpr"
                     /locus_tag="ACHUOF_000687"
     CDS             complement(944..1690)
                     /gene="fpr"
                     /locus_tag="ACHUOF_000687"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR0609991.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(1787..2797)
                     /gene="glpX"
                     /locus_tag="ACHUOF_000688"
     CDS             complement(1787..2797)
                     /gene="glpX"
                     /locus_tag="ACHUOF_000688"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR0609992.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            complement(3027..4517)
                     /gene="glpK"
                     /locus_tag="ACHUOF_000689"
     CDS             complement(3027..4517)
                     /gene="glpK"
                     /locus_tag="ACHUOF_000689"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR0609993.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            complement(4557..5402)
                     /gene="glpF"
                     /locus_tag="ACHUOF_000690"
                     /pseudo
     CDS             complement(4557..5402)
                     /gene="glpF"
                     /locus_tag="ACHUOF_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            5828..6073
                     /gene="zapB"
                     /locus_tag="ACHUOF_000691"
     CDS             5828..6073
                     /gene="zapB"
                     /locus_tag="ACHUOF_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR0609994.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            complement(6158..6643)
                     /gene="rraA"
                     /locus_tag="ACHUOF_000692"
     CDS             complement(6158..6643)
                     /gene="rraA"
                     /locus_tag="ACHUOF_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR0609995.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(6736..7662)
                     /gene="menA"
                     /locus_tag="ACHUOF_000693"
     CDS             complement(6736..7662)
                     /gene="menA"
                     /locus_tag="ACHUOF_000693"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR0609996.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            complement(7729..9060)
                     /gene="hslU"
                     /locus_tag="ACHUOF_000694"
     CDS             complement(7729..9060)
                     /gene="hslU"
                     /locus_tag="ACHUOF_000694"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR0609997.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            complement(9070..9600)
                     /gene="hslV"
                     /locus_tag="ACHUOF_000695"
     CDS             complement(9070..9600)
                     /gene="hslV"
                     /locus_tag="ACHUOF_000695"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR0609998.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            complement(9693..10652)
                     /gene="ftsN"
                     /locus_tag="ACHUOF_000696"
     CDS             complement(9693..10652)
                     /gene="ftsN"
                     /locus_tag="ACHUOF_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR0609999.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            complement(10744..11769)
                     /gene="cytR"
                     /locus_tag="ACHUOF_000697"
     CDS             complement(10744..11769)
                     /gene="cytR"
                     /locus_tag="ACHUOF_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR0610000.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            complement(11925..14123)
                     /gene="priA"
                     /locus_tag="ACHUOF_000698"
     CDS             complement(11925..14123)
                     /gene="priA"
                     /locus_tag="ACHUOF_000698"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR0610001.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            14326..14538
                     /gene="rpmE"
                     /locus_tag="ACHUOF_000699"
     CDS             14326..14538
                     /gene="rpmE"
                     /locus_tag="ACHUOF_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR0610002.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            complement(14599..15207)
                     /locus_tag="ACHUOF_000700"
     CDS             complement(14599..15207)
                     /locus_tag="ACHUOF_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR0610003.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(15391..15708)
                     /gene="metJ"
                     /locus_tag="ACHUOF_000701"
     CDS             complement(15391..15708)
                     /gene="metJ"
                     /locus_tag="ACHUOF_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR0610004.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            15985..17145
                     /gene="metB"
                     /locus_tag="ACHUOF_000702"
     CDS             15985..17145
                     /gene="metB"
                     /locus_tag="ACHUOF_000702"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR0610005.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            17148..19580
                     /gene="metL"
                     /locus_tag="ACHUOF_000703"
     CDS             17148..19580
                     /gene="metL"
                     /locus_tag="ACHUOF_000703"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR0610006.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(19719..19853)
                     /locus_tag="ACHUOF_000704"
     CDS             complement(19719..19853)
                     /locus_tag="ACHUOF_000704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610007.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            19929..20819
                     /gene="metF"
                     /locus_tag="ACHUOF_000705"
     CDS             19929..20819
                     /gene="metF"
                     /locus_tag="ACHUOF_000705"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR0610008.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            21148..23328
                     /gene="katG"
                     /locus_tag="ACHUOF_000706"
     CDS             21148..23328
                     /gene="katG"
                     /locus_tag="ACHUOF_000706"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR0610009.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            23422..24327
                     /gene="yijE"
                     /locus_tag="ACHUOF_000707"
     CDS             23422..24327
                     /gene="yijE"
                     /locus_tag="ACHUOF_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR0610010.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(24354..24961)
                     /locus_tag="ACHUOF_000708"
                     /pseudo
     CDS             complement(24354..24961)
                     /locus_tag="ACHUOF_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(25236..26337)
                     /gene="gldA"
                     /locus_tag="ACHUOF_000709"
                     /pseudo
     CDS             complement(25236..26337)
                     /gene="gldA"
                     /locus_tag="ACHUOF_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            complement(26348..27010)
                     /gene="fsa"
                     /locus_tag="ACHUOF_000710"
                     /pseudo
     CDS             complement(26348..27010)
                     /gene="fsa"
                     /locus_tag="ACHUOF_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(27022..29523)
                     /gene="ptsP"
                     /locus_tag="ACHUOF_000711"
                     /pseudo
     CDS             complement(27022..29523)
                     /gene="ptsP"
                     /locus_tag="ACHUOF_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            29832..30911
                     /locus_tag="ACHUOF_000712"
     CDS             29832..30911
                     /locus_tag="ACHUOF_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR0610011.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            30926..31246
                     /locus_tag="ACHUOF_000713"
     CDS             30926..31246
                     /locus_tag="ACHUOF_000713"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR0610012.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            31297..33594
                     /locus_tag="ACHUOF_000714"
     CDS             31297..33594
                     /locus_tag="ACHUOF_000714"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR0610013.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            33560..34438
                     /locus_tag="ACHUOF_000715"
     CDS             33560..34438
                     /locus_tag="ACHUOF_000715"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR0610014.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            34440..34781
                     /locus_tag="ACHUOF_000716"
     CDS             34440..34781
                     /locus_tag="ACHUOF_000716"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR0610015.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(34768..35619)
                     /locus_tag="ACHUOF_000717"
     CDS             complement(34768..35619)
                     /locus_tag="ACHUOF_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0610016.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(35768..37501)
                     /locus_tag="ACHUOF_000718"
     CDS             complement(35768..37501)
                     /locus_tag="ACHUOF_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR0610017.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(37684..40335)
                     /gene="ppc"
                     /locus_tag="ACHUOF_000719"
     CDS             complement(37684..40335)
                     /gene="ppc"
                     /locus_tag="ACHUOF_000719"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR0610018.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(40687..41838)
                     /gene="argE"
                     /locus_tag="ACHUOF_000720"
     CDS             complement(40687..41838)
                     /gene="argE"
                     /locus_tag="ACHUOF_000720"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR0610019.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            41992..42996
                     /gene="argC"
                     /locus_tag="ACHUOF_000721"
     CDS             41992..42996
                     /gene="argC"
                     /locus_tag="ACHUOF_000721"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR0610020.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            43004..43780
                     /gene="argB"
                     /locus_tag="ACHUOF_000722"
     CDS             43004..43780
                     /gene="argB"
                     /locus_tag="ACHUOF_000722"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR0610021.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            43841..45214
                     /gene="argH"
                     /locus_tag="ACHUOF_000723"
     CDS             43841..45214
                     /gene="argH"
                     /locus_tag="ACHUOF_000723"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR0610022.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT        10331 a        11703 c        12345 g        10947 t
ORIGIN      
        1 ttttccggga ttgttatttc cacgatgcaa atgacaatac ccggaaaatc cttctgaact
       61 cttcacctta agcaatatca aaaaaaatgg taatccataa gatcattact tgtttgttct
      121 tcccttaacg gcgcattatt cttaagtcgt aatcgacaga gaggcgaata tacagaggtg
      181 ccctatgaaa gatgtcgtag ataaatgcag tactaaagga tgtgcgattg atatcggtac
      241 ggtgattgat aacgacaact gtaccagtaa gttttcgcgc ttttttgcca cccgcgaaga
      301 agcagagtct tttatgacca aactgaaaga gcttgccgcc gctgcatcct ctgcagatga
      361 aggggccagc gtggcctata agattaagga tctggagggg caagttgagc ttgatgcggc
      421 cttcactttc tcatgccagg ccgagatgat tatttttgag ttatcactgc gttcgttagc
      481 ttgatggagt tgctttagaa ggagttaaac atggcttata aacacattgg cgtggcaatt
      541 tccgggaatg aagaagatgc cttactggtg aataaagccc tggagctcgc cagacataat
      601 gacgctcacc tgacgttaat tcatattgat gatggcttaa gcgagttgta tccgggtatc
      661 tacttccctg caacagaaga tattcttcaa ttgttgaaga ataagtcgga taacaagctg
      721 tataaactga cgaaaaatat tcaatggccg aagacaaaac tgcgtattga acgcggagaa
      781 atgccggaaa cactgctgga aattatgcaa aaagagcagt gcgacctcct tgtctgtggt
      841 catcatcact catttatcaa ccgtttgatg ccggcatatc gcgggatgat caataagatg
      901 agtgcggatt tgctcatcgt gccgtttatc gataagtaac cgcttaccag taatgctccg
      961 ctgtcatatg gcccggtcgg cgacgtaaat gtttcgtcat ctgccgggtc tctttcagca
     1021 actgttgtgt atcgcgcacc atctgtggat tgccgcacag catcacatgg ctggtttctt
     1081 tattcatcgg caggccaatc gcgctttcca gttccccact ttcaattaat gccggtatcc
     1141 gtccggtgag cgaccccgct gccgtttccc gactgaccac cgtctgaatg cgcagtttgc
     1201 cttcgtagcg tttttccagt tcttgcatca gtggcaaata gcttaagtcg gcggcataac
     1261 gtgcggcgtg aaccaggacc agatttttga agcgatctaa atctttgcct agttgcagaa
     1321 tcgataaata agggccaatc gctgtaccgg ttgccagcat ccatagcgtt tcgcagtccg
     1381 gtacttcatc cagaacaaag aagccagctg cttcgctaac cacctgcact tcatcgcccg
     1441 gtttcagtgc cgccaatcgt gggcttaatt tgccatcggg gacggtgacc aggtaaaact
     1501 ccagatcggg attatcgggc gagttaacat aggagtaggc gcgctggacg cgttcgccgt
     1561 cgatttcaag gccaagcttg gtaaattgcc cggcggtaaa cggaagcacg ggggcgtgaa
     1621 cggtgagact aaacagggcg tcggtccagt tctgcacttt agtgactttg cctgttaccc
     1681 aatcagccat gtttttctcc tgttttgatt gacttgcctt atcttcgttc tccgaacaaa
     1741 aaaattccag tcccgaagga ctggaaggct caatcgatca aatcactcag agaatgtgca
     1801 cctgcatttc cgggtctttg cgatccagat agtggatgga ctgaatgcgg cgaatggtgc
     1861 gtgacttgcc gcggatcagc agcgtttcgg tagtcgcgat attgcctttg cggctaatac
     1921 cttccagcag atcgcctttg gtaataccgg tggcagagaa gatgacgtta tcgctgcgcg
     1981 ccatatcgcc cagacgcaat actttaccgg cttcgatgcc catcgctttg cagcgtgcca
     2041 gctcctgctc gccaatgcga cgattctctt cgttgtcgcc tttgacgtca tgacgcgcca
     2101 gcagacgacc gttcatgtcg ccatctaatg cgcggatcac cgccgcagaa accacgcctt
     2161 ccggcgcgcc accaataccg tacagcacgt caacttcgct gtctggcata caggtgagaa
     2221 ttgaggccgc aacgtcgccg tccggaatag caaatacgcg tacgccgagt tgctgcattt
     2281 cagcgataac ggcatcgtgg cgtggtttag ccagaatcgt taccgtcagt tcgctcaacg
     2341 gtttgccgag cgccgccgct acattgcgca ggttatccgc cagcggcagg ttcagatcaa
     2401 tggtgccttt ggctcccggc ccgacaatca gcttctccat atacatatcc ggcgcattga
     2461 ggaagcagcc tttatcgcct actgccagca ccgccagcgc gttagcctgg cccatcgccg
     2521 tcatgcgcgt gccttcaatc ggatcaacag caatatctac cgcgtcgccg cgaccagtac
     2581 cgactttttc accaatgtag agcatcggtg cttcgtcgat ttcaccttca ccaatgacga
     2641 tggtgccgtc aatgttgacc tggttgagca taatacgcat ggcgtttacc gccgcgccgt
     2701 ccgcggtgtt tttatcgccg cgtcctaacc atttgtagcc agccagcgcc gcagattcgg
     2761 tgacgcggga aaattcgatg gcaagttctc gtctcatagc aaactctaag cagtaaggaa
     2821 tggcgcgaag tgtagcacag gggaagggtg ggattattga tgtgtgcggg gttgcccccg
     2881 cacggtttca gagtctgcgt gatgccggat gcggcgtgaa cgccttatcc cgcctacaca
     2941 aaatggcatt ggctcgtagg cctgataaga cgcgtaagcg tcgcatcagg cgatcagcat
     3001 caggcaatcg attcaggtgc ctgacattac tcgtcgtgtt cttcccacgc catcgcgcgt
     3061 ttgaccgctt ttttccagcc tgcgtaacgg taattacgct cagtggtttc gatgcctgga
     3121 cggaactcgc gctcaatcac cgctttctct tgcagctcgt cgaggttctg ccagaagcca
     3181 accgccaggc ctgcgagata ggccgcaccc aatgcggtga cttcgcgcac ttccgggcgc
     3241 tcaacgcggg tgccgagaat atcggactgg aactgcatca ggaaattgtt tgctactgcg
     3301 ccaccatcca cgcgcagggc gtgcagacgg ataccagagt cggcctgcat cgcttccagc
     3361 acgtcacgcg tctgataagc aatagactcc agcgtcgcgc gtataatgtg gttagcgttc
     3421 accccacgag tcagaccgaa aatcgccccg cgcgcatacg ggtcccagta cggcgcaccc
     3481 agcccggtaa atgccggaac cacatacaca ccattggtgt tttgcacttt ggtggcgaaa
     3541 tattcggaat cgtaggcgtc gttaatcaac ttcatctcat cgcgcagcca ctgaatggat
     3601 gcgcctgcca taaacaccgc accttccaac gcatagttca cttcgccagt cgggccgcag
     3661 gcgatggtgg tcagcaggcc gttttctgat ttcaccgctt tctcgccagt gttcatcagc
     3721 ataaagcagc cagtgccata ggtgttcttc gccatccctt ctttcacgca caactgacca
     3781 aacagcgcgg cctgctggtc accggcgatc ccggagattg gaatacgcgt gccgcctttg
     3841 ccgccaatgt tagtctgacc gtatacttcg gaagaacgac gcacttctgg cagcatctcg
     3901 cgcggaatat ccagcacttc cagcattttg tcgtcccagt ccagggtatg gatgttgaac
     3961 aacatggtac gagaggcgtt ggtgtaatcg gtcacatgga cacggccctg agtcattttc
     4021 cagataagcc acgtatcaac cgtaccaaac agcaattcac cacgacgtgc acgctcgtga
     4081 gagccttcca catggtcgag gatccacttc actttggtgc cagaaaagta cgggtcaatc
     4141 accagaccgg tattgctgcg gatataatct tctaaaccgt cacgttttaa atgctcgcag
     4201 atttctgcgg tacgacggca ctgccagaca atggcgttat agataggctt gccggtttct
     4261 ttttcccaga caatagtggt ttcacgctgg ttcgtaatac cgatagctgc aatttgatcg
     4321 gaactgatat cggctttcgc cagcacttct accagcgtgg agctttgggt ggcccagatt
     4381 tccattgggt cgtgttctac ccaacctggt tttgggtaga tttgctcaaa ttcgcgctgc
     4441 gacacgctaa tgatattggc atcgtgatcc attacgaccg cgcgggagct ggtggtgccc
     4501 tggtcgagcg caacgatata tttttttcag tcatgtttaa ttgtcccgta gtcatattac
     4561 agcgaagctt tttgttctga aggagttgtg gtttcctttt cttccacaac acagatatcg
     4621 caaggcaaat ggcgaccaat cagtttgcgg taggcaaatg cacctacaat cgcgccaacg
     4681 atagggctga aaagcggcac caggaagtaa ggaatgtctc tgccgccggt aaaggcgaca
     4741 ttgccccagc ccgccagcta ggcaaagact ttcggaccga agtcacgcgc tgggttcatg
     4801 gcaaaacctg tcaatgggcc catagatgcg ccaatgaccg caatcagtag accaatcagc
     4861 aagggagcca aagggccgcg tggtacaccg ttgccatcgt ccgttaacgc caggatcagc
     4921 cccatcagaa tagcggtaat caccatctca actgcgaaag cctgcacaaa attgatatga
     4981 ggattagggt aagtagagaa agtgccagcc agatcaacac tttcaacgct gccgcgaaca
     5041 atgtgatgag tctgctcgaa gtcgaaaaat aaattgtagt aaagcccgta aactaaagcc
     5101 gcagcgcaga aagcgccggc aacttgtgaa acgataaaag gaataacttt gcgcttgtcg
     5161 aaacaggcaa acagccacaa tgcaatggta acagcgggat taagatgcgc gccggaaacc
     5221 cctgcggtca ggtagatggc cattgccacc cccagtcccc aaatgacact gatttcccac
     5281 tgaccaaaag acgcaccagc gacttttagt gctgcaacgc aacccacacc gaaaaaaatc
     5341 aacaacccgg taccgaggaa ttcagcaatg cactggcctt tcaaggttga tgtttgactc
     5401 ataatcggat cctgaagagt taatgtttgt tgtatgcgtg aaagtcacgg acctccacga
     5461 tgcttgtagg catgctgtta acttatcgtt aacgagcaaa aacgagaaat atcgaactta
     5521 aaatgtgtgt gcttcgtcat aaaatgagcg ttatcgcgcc atttattcca tctgattgta
     5581 catgatgcat gtttgttaga tcattcgcgc aacaaattta ttaacatttt cgattgatgt
     5641 ggattatgtc actaggagaa ggatgaaaag tgtggcaaac cgtaatctgc aaggcgtgcc
     5701 gctggacagc gatggcgcgg cttcatacaa tcggagctaa ctaaagtgcg ctcgtattta
     5761 ttaaggcgtc accggtaatc gggacgagga ttttttatcc atcaacgcct tgcaattcag
     5821 gagaggtatg acaatgtcat tagaagtgtt tgagaaactg gaagcaaaag tacagcaggc
     5881 gattgatacc atcactctgt tgcagatgga aatcgaagag ctgaaagaaa aaaacaactc
     5941 actgtcgcag gaagttcaaa atgcccagca tcagcgcgaa gagctggagc gtgagaacaa
     6001 ccatctgaaa gaacagcaga acggctggca ggaacgtctg caggccctgc tgggtcgcat
     6061 ggaagaggtc tgattacgct tcatctttcg cgtaatttct ttactgtgta ggccaacagg
     6121 taacgcagaa aaaaggcacc ttgcggtgcc tttcttatca ttcaatatcc agcggatctt
     6181 ctgaaagaat aatcccggta ttgtcggcat aaagatggtc gccggagaag aaggtgacac
     6241 cgccaaaatt gacgcggaca tcgctttcgc caatgccttc gccagcggca ccaaccggaa
     6301 ttgccgccat cgcctggatg ccgatatcca actcttccag gtcatctacc tgacgcaccg
     6361 cgccgtaaat gaccagacct tcccattcat tttgtactgc cagacgcgcc agttcagcat
     6421 cgaccagtgc gcgacgaaca gaaccaccgc catcgacgac aagaacacga ccacggccat
     6481 tctgttcgag cagatcgtac agcaacccgt tgtcctcgaa acattttacc gtgattattt
     6541 gtccgccaaa cgacgcccgt ccgccaaagt tggagaacag cggttccacg acgttaacat
     6601 cttcttgata gatgtcacaa agctcggaag tatcgtattt cataggctta acattcagtt
     6661 gctgcgagaa ttttcagtat atcgcgctat gtgggctgtt ggcaaaatca tcaattgtta
     6721 attgatattt gtcagttatg ctgcccactg gcttaggaat atccctaaaa caaacagcag
     6781 gttagtcagt aacgctccct tgacagtacg ttccagcatt ggtcgcatcg ccaccgggtc
     6841 catttcccgc atcacataac gggcttgctt caccagtaat ggtgccgcca gcaggaacag
     6901 ccagccccac aggctatgca gcgaaaagag attaaacagc gccagacaca ccagcgagcc
     6961 catcagcagg caggcatgat aacgacgcgc gttcacttca cctaagcgca ccaccagcgt
     7021 gtttttgcca ttttcgcggt cgctattgat atcacgcagg ttattaatat tcagtactgc
     7081 cgttgccagc aggccgcatg cggtcgccgg aaggatcagt gccggaatca atgtatgagc
     7141 ctgtaaatac cagctcccca tgacactcaa ccagccaaag aaaaccagta cggaaatatc
     7201 acccagaccg atataaccat aaggacgatt gcccacggtg taggtgatag cggcaatgat
     7261 cgacaacccg ccaagaatca ggaaaccgac aaaatcggcc agcgtatggc atgccactgc
     7321 aaccagtgcc agcccggaga gacagatgag cacgacggta ataatgagcg cccgtttcat
     7381 cttttgctgg gtaatgaccc ctttttgcat gccgcgtagc ggcccaatgc ggtcaggttt
     7441 atcgctgcct tttaccgcat cgccgtaatc attggcgagg ttagaaagga tctgtaatag
     7501 cccggcggta attagtgcca gcagggcgac cagcggatcg aagtgacctt gccaccatgc
     7561 cagcgccgtc ccgacgataa ttgcagcaaa ggcgaggggg agggttttag gtcgtaaact
     7621 ttccagccac gcctgagttc ggctaatttg ttgttcagtc ataatacgcg ccaataaaaa
     7681 tggggccttt cagccccatc aaacaatgat gaaaatgatt gaacgcgatt ataggataaa
     7741 acggctcaga tcttcatctg ccaccaacgc atccagatgt ttgctcacat aatctgcgtc
     7801 aatagtgata ttttgaccgc ttaaatcgct ggcgtcgtag gaaatctctt ccattaaacg
     7861 ctccagaaca gtgtgtaaac gacgagcacc gatgttttcg gtagattcgt tcacctgcca
     7921 tgccgcttcc gcgatgcgtt taataccgga gtcggtaaac tcgatattta cgccttcagt
     7981 cgccatcagt gctttgtact gcacggtgat agaggcattc ggctcggtca gaatacgctc
     8041 gaagtcgctg gtggtcagtg cctgcagttc aacgcggatt ggcagccgac cttgcagttc
     8101 cgggatcagg tcagacggtt tcgcaatctg gaacgcgcca gaagcgataa acagaatgtg
     8161 gtcagttttg accatcccgt gtttggtgga aacggtgcaa ccttctacca gcggcagcag
     8221 gtcacgctga acgccttcac gagaaacatc cggaccggaa gactcgccgc gcttacagat
     8281 tttgtcgatt tcgtcgataa acacgatccc gtgctgctca acagcgtcga tagcgtcttg
     8341 cttcagctct tccgggttca ccagtttcgc cgcttcttct tcaatcagca gcttcatggc
     8401 gtctttgatt ttcagcttac gcgctttttg cttctggccg cccaggttct ggaacatgga
     8461 ctgcagctgg ctggtcatct cttccatgcc cggaggagcc ataatttcaa cgcccatcgg
     8521 tgctgcggca agatcgatct cgatttcttt gtcatcaagc tggccttcac gcagtttttt
     8581 gcggaatgcc tgacgagcag cggaaggttc ctgctgctgt tcagtctgtc cccagttgtt
     8641 tttagcaggt gggatcagca cgtcgagaat acgttcttct gccagttctt cagcgcgata
     8701 acggtttttc tcgatagcct ggacgcgtac cattttcacg gcggcatcgg tcagatcgcg
     8761 aataatagaa tcaacttcct taccgacgta gcccacttcg gtaaatttgg tcgcttcaac
     8821 tttgatgaac ggcgcattcg ccagcttagc cagacgacgg gcgatttcag ttttaccgac
     8881 accggtcggg ccgatcatca ggatattttt cggggtcact tcatggcgca gctcttcgtt
     8941 gagctgcatg cgacgccagc ggttacgcag agcaatcgcc acagaacgct tggcgttgtc
     9001 ctggccgatg atgtgcttat ccagttcgct gacgatttcg cgtggggtca tttcagacat
     9061 gggagatcct tacgctttgt agcttaattc ttcgatggtg tggaaatggt tggtatagat
     9121 gcaaatgtcg cctgcaatat ccaacgcctt ttcagcaatt tcacgggcgc taagttcagt
     9181 gttttctaac agcgcgcgcg ccgcagcctg ggcgtaaggg ccgccggagc cgatagcaat
     9241 aagatcgttt tctggctgca ccacgtcacc gttaccggtg atgataagcg atgcagtttc
     9301 atccgcgact gccagcagtg cttcaagttt gcgcagcatg cgatcggtac gccagtcttt
     9361 tgccagctca acggcggctt taaccagatg gccctgatgc atttccagtt tacgttcaaa
     9421 cagttcgaac agcgtaaaag catccgcagt accgcccgca aagcccgcga tgactttgtc
     9481 gttgtacaga cggcggacct ttttcacgtt gcctttcatt acggtattgc ccaacgtggc
     9541 ctgaccatca ccagcgatga ccacatggcc gttacggcgt acgcttacta tagttgtcac
     9601 gagctgaccc cttggttacg aatacagagt acaaaccccg tacaaaagta cggggcataa
     9661 tgcaattata gatggggggg attttgaggg tttcaacccc cggcggcgag ccgaatgcag
     9721 tttgtatgac ccgccatctt caaccgattg agggtgctgt ctgcgttctc tttgcctttc
     9781 accgggccaa tgaccacacg attccagcca ttgttggtgg tgattttcga gtcaaatcct
     9841 tcgaacgcca gctgagcacg taccgtctct gcctgttccg cgcctctgaa cgaaccgcac
     9901 tgcaccatcc agcggcgttc gtcttttttc tccgccgtcg gttttggcgc gtcagcggca
     9961 cgagcaaccg gcgcagcttg ctgcggcttc gactgcgcgg tcgtgtgcgc aggggtttgc
    10021 agcagatcct ggtacggctg ctgagtagaa gctggtttag actggcgcgg ttgcgcctgt
    10081 accggcgcgg cttgcgacgt acgcgtctgc tgctgccagc tttgttcagt cgtacgcgac
    10141 tgctgagcca ggcgttgctg ctccgctaat tgctgcgcct gacgctggcg ttgtagcgtt
    10201 tgctgacgct gttccggcgt ctgttcattc cacggaacct caaccagctg cgttggctgc
    10261 tggcgcatat cagcctgcat ttgttcaaga agctgacgtt gttctggtgt cagttgctcc
    10321 ggcgttttca cttcaccacc ggcagaaggt tctgtgggcg cacgcacacc cggctggcga
    10381 ctttccagct ctttaatgta gcgccagcgt tcttctggtt ttggtggtag tccgtttccg
    10441 gtcactttct ggctttgcag cgtctcggac tcttctttct tgtgatgcgt aatgaagtac
    10501 agaccaccga taaaggtcac aagaacggcg gcagcaatag cgaccatagc gggagaaacc
    10561 gcaggcagat ttcgttgctt tttccgtgag gtgctctttt ttcgccgcga aggtgccggt
    10621 tggctgcggc gtacataatc tcgttgtgcc actatcgttt cgctgtattt attcgttcgt
    10681 cagcccgcca tgttacttaa gcggcgggcc tttgaccagt cacggcagtc ttaaaggttt
    10741 actttaaggt aacgcgcgtg ttgatccccg gatgataagt tcgcagtcca ttaaacgaga
    10801 gccactgcca acgtgttgcc cctgcatttg atcaagcaat aacagcatag cttcccgacc
    10861 gatttcgtaa cgcggctgcg cgatggttgt cagcggcgga tcacaaaatt gcgtcaggtc
    10921 gatgttatca aaaccgatta tggaaaggtc ttccgggact ttcagcccct ggcgttttgc
    10981 ctgagaaagt gcgccgagcg ccatcacatc gctatggcag aagactgcag taggcggttg
    11041 tggaagatca agcagctgtt gcattgcttt gcttccggct tcgaaggtga agtcgccacg
    11101 ggcgatgtat tgtggatcaa ccataattcc gcagcgacgc agcgcctgaa catagccttg
    11161 caggcggtag tgacacagtg gcatctcttc gggaccggct atacagccaa tccgtttatg
    11221 cccttgctca tataaataat ttactgcatc aaatgcggcg gtcagattgt cgatatgaac
    11281 ggtaggcagc tccagttccg gtgcaaattc gttcgccatc accatcggcg gcagattacg
    11341 ctgttcctca atgctggcat caaacggcag ccttgaaccc agcagcaaca tgccatcaat
    11401 ttgcttggtg atgatcaaat cgataaaggt tttttcctgc tgattttgat gcgcacagtc
    11461 gccaatcagc accagatatc cgtgatttgc cgccgtaact tcgataccgc gaataatttc
    11521 gctaaagaag ggatcgcaga tatccgggac aatcaccaga atggtgcggg attcattacg
    11581 cttgacgttg cgccccatag gctgcggtaa ataacccact tcccgggccg ctttttcaac
    11641 ccgattacgg gtggcctggg agactttatc gggattcatt aatgctcggg agacagtcgc
    11701 tgtagagact tttgccttga gggcaacgtc tttcatggtc gcggcagttt cctgcttctt
    11761 cgctttcaca ctcactcctc gcctggcacg tcaggcgtac tacatccatg tttacttcac
    11821 atcggcaaca tttttagcag atagcgcgtg aaaacggtta cagaattttc atgaaaagtg
    11881 tgatgaatat tgaatttttc gatccgcctc gcatcgtaag cggtttaacc ctcaatcgga
    11941 tcaacatcca gtacccattt caccttgcgg gaatccggta ttgtattgat gagcgccagc
    12001 gtaccactaa tgatgtgttg caagcgcacg cgggaagggt gctgcaacaa tatctgccag
    12061 cgccagcgac cgccacgttt aggtgccagt gccggaaccg gaccgagaac ccacagtttg
    12121 tcgtctgcca gcgggctgga gaggatcaga ttacgcagtt gttgcaggaa cagcggcgca
    12181 tgctgattgt tatgatcttc cgcacgcaca atcacatggc tggtccatgg cggcagttgc
    12241 atcatccgtc gctcagccag cgcctgttcg gcaaaggcat cgtagccttt atagagcaac
    12301 gtttgcaaca gaggatgttc cggatggtgc gtttgcagca ccacttcgcc ttgtttaccc
    12361 gcacgcccgg cacgaccagc gacctgggtg taaagctgag cgaaacgctc tgccgagcga
    12421 aaatcggcag aaaacagcgc gccgtccaca tccagtaacg ccaccagcgt cacatccgga
    12481 aagtggtgac ctttcgccag catttgtgtg ccgatcaaaa tcctcgcacc gccgcgatgt
    12541 acttccgcca gttgctgttc cagcgcccct ttgcggctgg tggtatcgcg gtcgatacga
    12601 gaaatgggca catcagggaa cagcggcgca agcgtctgtt caagctgttc ggtgcccagc
    12661 cccacgggta ccaggtgcgt ggaaccgcag gaagggcact ggcgcggcac cggacgctga
    12721 ctgtcacagt ggtgacagcg caaatgctgt tgcgcctgat gcagcgtgta gtagtgatcg
    12781 caacgtgggc attcggcaat ccagccgcag tcgtggcaca gtagcgcagg cgcaaagcca
    12841 cggcggttaa gaaagaggat aacctgattg ttggcctgta aatgctggcg catacgggtg
    12901 attaacgccg gagccagacc tgcctgcacc ttctgacctt ttaaatccag cacgtgttga
    12961 attgccggac gcgcattccc cgcccggcgc gtcaggcgca gcaggcggta ttttttctgc
    13021 tggacgttgc acaacgtttc cagcgcgggc gttgcggagc caagaataat cgggatttgc
    13081 tcgctatgcg cacgatacac cgccaggtcg cgggcatgat agcgccagcc ttcctgctgc
    13141 ttgtaggaac tgtcgtgctc ttcgtcaatg acaatcacgc cgagattttt aaacggcgta
    13201 aacagcgcgg agcgggtgcc gatcacaatc gccgcctcgc catttttcgc tttcagccac
    13261 gccgaaagac gctcgctgtc gttcagaccg gaatgcagaa cttccacggg cgcattaaac
    13321 cgttcacgaa aacgggcgat agtttgcggt gtcaggccga tttccggcac catcaccagc
    13381 gcctgtttgc cctgagcgag cacgttttcc agtacactga gataaacctc cgttttaccg
    13441 gaaccggtaa cgcccgccag cagccaggca gaaaaagtat ctgccgcgct atgaattgcg
    13501 ccaacggcgg tggcctgttc ggtattcaat cgcaaccgct caccagaaac ggcatagttc
    13561 gttcgccagt cgctaaactc tggtgtttca cttgctaaat cacacagacc ttttttgcgc
    13621 agcgcctgca acgcggcatc attaaattcg agcgtggcaa cctggtcgcg ccagattttg
    13681 ccttgccgta acgctgccag cgcctgttgt tgcttgggtg agcgtttcag gctgttcaga
    13741 tccaccgcct gaccttgttc agtggcaaac cagtaccaca tcggcgcgtt cgccgcaggc
    13801 cgcccctggc gtagtaaaat cggcaaggca tgaaacagca catcgccaat cggatgatga
    13861 tagtaatctg ccgcccatag cagcaatcgc cagacggagt gagtaaacac cggctcacca
    13921 tccagcactt cgactaccgc ttttagctca gtgagcggca gttcgctggc atcgctaact
    13981 gataccacaa tcccgatgcg ctcctgctgt ttgccaaacg gcacgcgcac gcgacaccca
    14041 gctttaaccg tcatgccttc tggcagcaga tagtcaaagg tacgaggaag cggaacgggc
    14101 aaggcaacgt gggcaacggg catagcatca tcctgacttg aaattcggtg ggttagtata
    14161 cacattgccg tagaagagtg cggatcagtt tgcatacgct ggttaatttc tgtatgattt
    14221 cgcgccttcg tacgaaatga tcgtattgaa gctatacttt taacatcgcg tggtgtctgg
    14281 cgttagggct ggaagagcga cgcggcctta aaccgaggtt ttcccatgaa aaaagatatt
    14341 cacccgaaat acgaagaaat tactgctagc tgctcttgcg gtaacgtaat aaaaatccgc
    14401 tccaccgttg gtcatgacct gaacctcgac gtgtgcagca agtgccaccc gttcttcact
    14461 ggcaaacagc gtgatgttgc taccggtggc cgtgttgacc gcttcaacaa gcgtttcaac
    14521 atcccgggca gcaaataaga ccggatttcc gaaaaaagcg ccgcacggcg ctttttttgt
    14581 gcctgaattt tggtcgtatc accacgaaaa cagcggccac tctttggcaa ccgtggtgag
    14641 ttgtggcgcg tcgaaaacgg cttgtggtga aactaccgtt tcctcaagcg gaatattttt
    14701 accgtagcgt tctttgagct tcgcctggac cagcgggttg ctgagatcaa actctttcaa
    14761 cttctgctgt tcgcccacgc gcattcccag cgcgttctgg taaaccttcc acaccacttc
    14821 cgaacagtac tggcgatcgt cgctccatga gaagctgaaa tcatacggtt taccgagata
    14881 acgttttgcc gtttgcgtca gtttttgctg ttgttcaaca ctcagtccgc cttcaacgcg
    14941 gcgaacaacg tagttgccct tttcaccatg ggcaatccac tgcttgagcg gggtgtattt
    15001 caccgggccg accgcttcaa aaacgtaggg ctttttgtta cgcatcacca gcataccggt
    15061 gtggctgtaa tcggaatggg ttgccagttg gatcgcttta ctttgcgatg agcgagagat
    15121 ttgaaagatg atgtcgccag tttgcggctg ccaggcaaag gcaggtacag aaaccagcag
    15181 gctgaggatc agcagcctgt ttttcatagt taaacgtcca tgtataaaaa gcggtgggtc
    15241 gcagacaacg tgctcgttgt ttatgccgga tgcggcgtga acgccttatc cggcctacaa
    15301 gttcgtgcaa attcaataaa ttgcaatatg acgtaggcct gataagcgta gtgcatcagg
    15361 cgattccact ccgcgccgct cttttccgct ttagtattcc cacgtctccg ggttaatccc
    15421 catctcacgc atgatctctt ttgccgcttc cgggatttcg tcgctgcgct ctttacgcag
    15481 atcggcatca tccggcaaag gttgcccggt aaaggcatgc agaaacgctt cgcacagcag
    15541 ctcgctgttg gtagcgtgac gcaggttgtt cacctgacga cgcgtgcgtt catcggtgag
    15601 gatttttaac accttaagag gaatggaaac cgtaatcttt ttgacttgtt cactcttctt
    15661 gccgtgctca gcgtatgggc tgatatattc gccgctccat tcagccatga gatacttaat
    15721 cctcttcgtc aataaattga gaccagaccc cacaatttat gtgggtactg accgtaaacc
    15781 cgcatagttt accgtacagg cgttaccgtg acatcgtgta atgcacctgt cggcgtgata
    15841 atgcatataa ttttaacggc tatttgggat ttgctcaatc tatacgcaaa gaagtttaga
    15901 tgtccagatg tattgacgtc cattaacaca atgtttactc tggtgcctga catttcaccg
    15961 acaaagccca ggaaacttca tcacatgacg cgtaaacagg ccaccatcgc agtgcgtagc
    16021 gggttaaatg acgacgaaca gtatggttgc gttgtccctc ccatccatct ttccagcacc
    16081 tataacttta ccggatttaa tgaaccgcgc gcgcatgatt actcgcgtcg cggcaaccca
    16141 acgcgcgatg tggttcagcg tgcgctggca gaactggaag gcggtgctgg cgcagtactt
    16201 actaataccg gcatgtccgc gattcacctg gtaacgaccg tctttttgaa acctggcgat
    16261 ctgctggttg cgccgcacga ctgctacggc ggtagctatc gcctgttcga cagtctggcg
    16321 aaacgcggtt gctatcgcgt gttgtttgtt gatcaaggcg atgaacaggc attacgggca
    16381 gcgctggcag aaaaacccaa actggtactg gtagaaagcc caagtaatcc attgttacgc
    16441 gtcgtggata ttgcgaaaat ctgccatctg gcaagggaag tcggggcggt gagcgtggtg
    16501 gataacacct tcttaagccc ggcattacaa aatccgctgg cattgggtgc cgatctggtg
    16561 ttgcattcat gtacgaaata tctgaacggt cactctgacg tggtggctgg cgtggtgatt
    16621 gctaaagacc cggacgttgt cactgaactt gcctggtggg caaacaatat tggcgtgacg
    16681 ggcggcgcgt ttgacagcta tctgctgcta cgcgggttgc gaacgctggt gccgcgtatg
    16741 gagctggcgc agcgcaacgc gcaggcgatt gtgaaatatc tgcaaaccca gccgttggtg
    16801 aaaaaactgt atcacccgtc gctgccggaa aatcaggggc atgaaattgc cgcgcgtcag
    16861 caaaaaggct ttggcgcaat gttgagtttt gaactggatg gcgatgagca gacgctgtgt
    16921 cgtttcctgg gcgggctgtc gttgtttacg ctggcggaat cattaggggg agtggaaagt
    16981 ttaatctctc acgccgcaac catgacacat gcaggcatgg caccagaagc gcgtgctgcc
    17041 gccgggatct ccgagacgct gctgcgtatc tccaccggta ttgaagatgg cgaagattta
    17101 attgccgacc tggaaaatgg cttccgggct gcaaacaagg ggtaaaaatg agtgtgattg
    17161 cgcaggcagg ggcgaaaggt cgtcagctgc ataaatttgg tggcagtagt ctggctgatg
    17221 tgaagtgtta tttgcgtgtc gcgggcatta tggcggagtt ctctcagcct gacgatatga
    17281 tggtggtttc cgccgccggt agcaccacta accagttgat taactggttg aaactaagcc
    17341 agaccgatcg tctctctgcg catcaggttc aacaaacgct gcgtcgctat cagtgcgatc
    17401 tgattagcgg tctgctaccc gctgaagaag ccgatagcct cattagcgct tttgtcagcg
    17461 atcttgagcg cctggcggcg ttgctcgaca gtggtattaa cgatgcggtg tatgcggaag
    17521 tggtgggcca cggtgaagtg tggtcggcgc gcctgatgtc tgcggtactt aatcaacaag
    17581 ggctgccagc ggcctggctt gatgcccgtg agtttttacg cgctgaacgc gctgcgcaac
    17641 cgcaggttga tgaagggctt tcttacccgt tgctgcaaca gctactggta caacatccga
    17701 gcaaacgtct ggtggtgacc gggtttatca gccgtaacaa cgccggtgaa acggtgttgc
    17761 tggggcgtaa cggttccgac tattccgcga cacaaatcgg tgcgctggcg ggtgtttctc
    17821 gtgtaaccat ctggagcgac gtcgccgggg tatacagtgc cgacccgcgt aaagtgaaag
    17881 atgcctgcct gctgccgttg ctgcgtctgg atgaggccag cgaactggcg cgtctggcgg
    17941 ctcccgttct tcacgcccgt actttacagc cagtttctgg cagcgaaatc gacctgcagc
    18001 tgcgctgtag ctacacgccg gatcaaggtt ccacgcgcat tgaacgcgtg ctggcctccg
    18061 gtactggtgc gcgtattgtc accagccacg atgatgtctg tttgattgag tttcaggtgc
    18121 ccgccagtca ggatttcaaa ctggcgcata aagagatcga ccagatcctg aaacgtgccc
    18181 aggtgcgtcc actggcggtt ggcgtgcata acgatcgcca gttgttgcaa ttttgctaca
    18241 cctcagaagt ggccgacagt gcgctgaaaa tcctcgacga agcgggatta cctggagaac
    18301 tgcgcctgcg tcaggggctg gcgctggtgg cgatggtcgg ggcgggtgtc acccgtaacc
    18361 cgctgcattg ccaccgcttc tggcagcaac tgaaaggcca gccggtcgag tttacgtggc
    18421 agtccgatga cggcatcagt ctggtggcag tactgcgcac cggcccgacc gaaagcctga
    18481 ttcaggggct gcatcagtcc gtcttccgcg cagaaaaacg catcggcctg gtattgttcg
    18541 gcaaaggcaa tatcggctct cgctggctgg aactgttcgc ccgcgagcag agcacgcttt
    18601 cggcgcgtac cggctttgaa tttgtgctgg caggcgtggt ggacagccgc cgcagtctgt
    18661 tgagttatga cgggctggac gccagccgcg cgttagcctt cttcaacgat gaagcggtcg
    18721 agcaggatga agagtcgctt ttcctgtgga tgcgcgccca tccgtatgat gatttagtgg
    18781 tgttggacgt taccgccagc cagcagcttg ctgatcagta tctcgatttc gccagccacg
    18841 gtttccacgt catcagtgcc aacaaactgg cgggagcaag cgacagcaat aaatatcgcc
    18901 agatccacga cgccttcgaa aaaaccgggc gtcactggct atacaacgcc accgttggtg
    18961 cgggcttgcc gatcaaccat accgtgcgcg atctgatcga cagcggcgat accattttgt
    19021 cgatcagtgg gatcttctcc ggcacgctct cctggctgtt cctgcaattc gacggtagcg
    19081 tgccgtttac cgagctggtg gatcaggcgt ggcagcaggg cttaaccgag cctgatccgc
    19141 gtgacgacct ctccggcaaa gacgtaatgc gcaagctggt gattctggcg cgtgaagcgg
    19201 gttacaacat tgaaccggac caggtacgtg tggaatcgct ggtgcctgct cattgcgaag
    19261 gcggcagcat cgaccatttc tttgaaaatg gcgatgaact taacgagcag atggtgcaac
    19321 ggctggaagc ggcccgcgaa atggggctgg tgctgcgcta cgtggcgcgt ttcgatgcca
    19381 acggcaaagc gcgtgtgggc gtggaggcgg tgcgtgaaga ctatccgctg gcatcactgc
    19441 tgccatgcga taacgtcttt gccatcgaaa gccgctggta ccgcgataat ccgctggtga
    19501 tccgtgggcc tggcgctggg cgcgacgtca ccgccggggc gattcagtcg gatatcaacc
    19561 ggctggcaca gttgttgtaa tttcagaaat ttaataatgc ccggtactca tgttttcggg
    19621 tctatggttt ctaatgaaat atattgaatt atcatagaat taggccggat gaagcgttta
    19681 cgacgaatcc ggcaagaagc aataagtaca tggttagttt atatttgcag tccggtttgc
    19741 tttgcatatc ggattttctt tttcttacca tcctgaagtt ttttcatctt ccctgatttt
    19801 tcctcaccat cattggtcat ttttcggttg acgcccttcg gcttttcctt catctttaca
    19861 tctggacgtc taaatggata gatgtgcaca acacaacata taactacaag cgattgatga
    19921 ggtaaggtat gagctttttt cacgccagcc agcgggatgc cctgaatcag agcctggcag
    19981 aagtccaggg gcagattaac gtttcgttcg agtttttccc gccgcgtacc agtgaaatgg
    20041 agcagaccct gtggaactcc atcgatcgcc ttagcagcct gaaaccgaag tttgtatcgg
    20101 tgacctatgg cgcgaactcc ggcgagcgcg accgtacgca cagcattatt aaaggcatta
    20161 aagatcgcac tggtctggaa gcggcaccgc atcttacttg cattgatgcg acgcccgacg
    20221 agctgcgcac cattgcacgc gactactgga ataacggtat tcgtcatatc gtggcactgc
    20281 gtggcgatct gccgccggga agtggtaagc cagaaatgta tgcttctgac ctggtgacgc
    20341 tgttaaaaga agtggcagat ttcgatatat ccgtggcggc gtatccggaa gttcacccgg
    20401 aagcaaaaag cgctcaggcg gatttgctta atctgaaacg caaagtggat gccggagcca
    20461 accgcgcgat tactcagttc ttcttcgatg tcgaaagcta cctgcgtttt cgtgaccgct
    20521 gtgtatcggc gggcattgat gtggaaatca tcccgggcat tttgccggta tctaacttta
    20581 aacaggcgaa gaaatttgcc gacatgacca acgtgcgtat tccggcgtgg atggcgcaaa
    20641 tgtttgacgg gctggatgac gatgccgaaa cccgcaaact ggtgggcgcg aatattgcca
    20701 tggatatggt gaagatttta agccgtgaag gagtgaaaga tttccacttc tatacgctta
    20761 accgcgctga aatgagttac gcgatttgcc atacgctggg tgttcgacct ggtttataaa
    20821 tagtgtggct tttgtgaaaa tcacacagtg atcacaaatt ttaaacagag cacaaaatgc
    20881 tgcctcgaaa tgagggcggg aaaataaggt tgtcagcctt gttttctccc tcattacttg
    20941 aaggatatga agctaaaacc cttttttata aagcatttgt ccgaattcgg acataatcaa
    21001 aaaagcttaa ttacgatcaa tttgatctac atctctttaa ccaacaatat gtaagatctc
    21061 aactatcgca tccgtggatt aattcaatta taacttctct ctaacgctgt gtatcgtaac
    21121 ggtaacactg tagaggggag cacattgatg agcacgtcag acgatatcca taacaccaca
    21181 gccactggca aatgcccgtt ccatcagggc ggtcacgacc agagcgcggg ggcgggcaca
    21241 accactcgcg actggtggcc aaatcaactc cgtgttgacc tgttaaacca acattctaat
    21301 cgttctaacc cgctgggtga ggactttgac taccgcaaag aattcagcaa attagattac
    21361 tacggcctga aaaaagatct gaaagccctg ctgacagaat ctcagccgtg gtggccagcc
    21421 gactggggca gctacgccgg tctgtttatt cgtatggctt ggcacggcgc aggtacttac
    21481 cgttcaatcg atggacgcgg cggcgcgggt cgtggtcagc aacgttttgc accgctgaac
    21541 tcctggccgg ataacgtaag cctcgataaa gcgcgtcgcc tgttgtggcc aatcaaacag
    21601 aaatatggtc agaaaatctc ctgggccgac ctgtttatcc tcgcaggtaa cgtggcgctg
    21661 gaaaactccg gcttccgtac cttcggtttt ggtgccggtc gtgaagacgt ctgggaaccg
    21721 gatctggatg ttaactgggg tgatgaaaaa gcctggctga ctcaccgtca tccggaagcg
    21781 ctggcgaaag caccgctggg tgcaaccgag atgggcctga tttacgttaa cccggaaggc
    21841 ccggatcaca gcggcgaacc gctttctgcg gcagcagcta tccgcgcgac cttcggcaac
    21901 atgggcatga acgacgaaga aaccgtggcg ctgattgcgg gtggtcatac gctgggtaaa
    21961 acccacggtg ccggtccgac atcaaacgta ggtcctgatc cagaagctgc accgattgaa
    22021 gaacaaggtt taggctgggc gagcacttac ggcagcggcg ttggcgcaga tgccattacc
    22081 tctggtctgg aagtagtctg gacccagacg ccgatccagt ggagcaacta tttcttcgag
    22141 aatctgttca agtatgagtg ggtacagacc cgcagtccgg ctggagcaat ccagttcgaa
    22201 gcggtagacg caccggaaat tatcccggat ccgttcgatc cgtcgaagaa acgtaaaccg
    22261 acaatgctgg tgaccgacct gacgctgcgt tttgatcctg agttcgagaa gatctctcgt
    22321 cgtttcctca acgatccgca ggcgttcaac gaagcctttg cccgtgcctg gttcaaactg
    22381 acgcacaggg atatggggcc gaaatctcgc tacatcgggc cggaagtgcc gaaagaagat
    22441 ctgatctggc aagatccgct gccgcagccg atctacaacc cgaccgagca ggacattatc
    22501 gatctgaaat tcgcgattgc ggattctggt ctgtctgtta gtgagctggt atcggtggcc
    22561 tgggcatctg cttctacctt ccgtggtggc gacaaacgcg gtggtgccaa cggtgcgcgt
    22621 ctggcattaa tgccgcagcg cgactgggat gtgaacgccg cagccgttcg tgctctgcct
    22681 gttctggaga aaatccagaa agagtctggt aaagcctcgc tggcggatat catcgtgctg
    22741 gctggtgtgg ttggtgttga gaaagccgca agcgccgcag gtttgagcat tcatgtaccg
    22801 tttgcgccgg gtcgcgttga tgcgcgtcag gatcagactg acattgagat gtttgagctg
    22861 ctggagccaa ttgctgacgg tttccgtaac tatcgcgctc gtctggacgt ttccaccacc
    22921 gagtcactgc tgatcgacaa agcacagcaa ctgacgctga ccgcgccgga aatgactgcg
    22981 ctggtgggcg ggatgcgtgt actgggtgcc aacttcgatg gcagcaaaaa cggcgtcttc
    23041 actgaccgcg ttggcgtatt gagcaatgac ttcttcgtga acttgctgga tatgcgttac
    23101 gagtggaaag cgactgacga atcgaaagag ctgttcgaag gacgtgaccg cgaaaccggc
    23161 gaagtgaaat acacagccag ccgtgcggat ctggtatttg gttctaactc tgtcctgcgt
    23221 gcagtagcgg aagtttacgc cagcagcgat gcccacgaga agtttgttaa agacttcgta
    23281 gcggcatggg tgaaagtgat gaacctcgac cgtttcgacc tgctgtaatc tgaccttctt
    23341 cagcgactgt ctttcaggca gtcgctgaag tttctttacc ggcgtatagt gtccacagga
    23401 aaactacaca ctggatctct catgtctgcc gcaggaaaga gcaacccact ggcaatcagt
    23461 ggcttggttg tgctcacact tatctggagt tatagctgga ttttcatgaa gcaagtcacc
    23521 agttacatcg gtgccttcga ctttaccgcc ttacgctgca ttttcggcgc tctcgtttta
    23581 ttcatcgtcc ttttattacg tggtcgcgga atgcgcccga cgccgtttaa atacacctta
    23641 gccattgccc tgttacaaac ctgcgggatg gttggtctgg cgcagtgggc gttggtcagc
    23701 ggaggtgcgg ggaaggtggc gatcctgagc tataccatgc cgttctgggt ggtgattttc
    23761 gccgcgttgt ttctcggtga acgcctgcga cgtgggcaat atttcgcgat tctgattgcc
    23821 gctttcggct tatttttggt gttgcagccg tggcaactcg atttctcttc gatgaaaagt
    23881 gccatgctgg caatcctctc cggtgttagt tggggggcga gcgcgattgt tgctaaacgc
    23941 ctatacgccc gtcatccgcg cgtggattta ttatcgttaa catcctggca gatgctgtat
    24001 gcggcgctgg tgatgagtgt ggtcgcttta ctggtgccgc aacgtgaaat tgactggcag
    24061 cccaccgtgt tctgggcgct ggcctacagt gcgattctgg cgacggcgct agcgtggagc
    24121 ttatggctgt ttgtgctgaa aaacttgcct gccagtattg ccagcttaag cacactggcc
    24181 gttcccgttt gtggcgtact cttttcctgg tggctgctcg gtgagaatcc gggggccgtt
    24241 gaaggtagcg gtattgtgct gattgtgctg gcactggcgc tggtgagccg taagaaaaaa
    24301 gaagctgtca gtgtaaaaag catctgaatt ttttcttcat gtggggcgat ctcttattta
    24361 acaaaataac gataatgccc caccatccgc cagctaaaca gcacatcttc ttcctgcgcg
    24421 cctgcgccaa tgttatgtat caccagcggc gtaccgtcgc gggcgaagcc atctgacgcc
    24481 accccaatat gcgccagccc gttatctaat cgccaggaga caatatcgcc cgcttgatag
    24541 tcaccggcat ttttactgat ggggcgtgtt ttgttgtggc ggctaaacca ggtttccaga
    24601 ttaggcaccc gacggtgatc gatgttgctg tccgggcgct ttaacttcca tttttgcggg
    24661 tactcagcaa aattcttcgc catatcttcg tgaaccagtt tctgcaaatc gaccttctgg
    24721 ctgcgcaatg cgcggatcac catatcggaa catacaccgc gttcttgcgg aacatcaccg
    24781 ccaggatagg taagctgcga tcgtaaaata gcgtgctgcc aatttgctgt ctggcaccgt
    24841 ctgcgatggc aaggttggta ttggcctgga tttgtaccac ggttggtgga acggcgggag
    24901 attttaagga gtggctggta aatgccgtta gcaggctgag cagcgccagt gaagctttca
    24961 tctggttcgt ccgtgaaaat aagagtcaca gattcgacct tccgggctaa cgattgttct
    25021 gtggagaaaa tgtccagaag cggataaaaa actcctgaac aacgtgggag tttcagatta
    25081 ctggcacaga gtgccggatg cggagtgaac gccttatccg gcctacaaaa gcacgcaaat
    25141 tcaatatatt gcagagattg cgtaggcctg ataagcgtag cgcatcaggc aattttgcgt
    25201 tcaaactccc ggacaagccg ggagtttgga gtaggttatt cccactcttg caggaaacgc
    25261 tgaccgtact ggtcagctac cagcagtgcg gcgtaaacct gatctggcgt cgcgccgcca
    25321 ggcatgttgt ggatggtttc accttctgca caagatgctt ttgcaacaat gcgcattttc
    25381 gccgggacat cttctttaat atccagttga gcgagagtta ttggcaaacc taccgcatgg
    25441 ctaagtgcag ctacggtttc gatttcctcc accggcgcgt tttccagtac cagctgcgtc
    25501 agcgtaccaa atgccacttt tcaccgtgat aatagtgatg cgcgtccggg atagcggtca
    25561 ggccgttatg caccgcgtga gccgccgcca gccgccgctt tcaaaaccaa cgccgctcaa
    25621 ataggtgttc gcttcaatca cgcgctccag cgccggggtt actacatgct gttcggcggc
    25681 aagcatcgct ttttcgcctt cttccagcag ggtgttgtag cacagttcag ccagtgccag
    25741 cgccgcctgg gtgcacttgc cgcccgccat ggtggtcgcg ccgctacgag agcaagcacg
    25801 cgcttcaaac caggttgcca gcgcatcgcc gatacccgct gctaacagac gtgcaggtgc
    25861 gcccgcgacg attttggtgt cgacaatgac catattcggg ttatttggca acagcagata
    25921 gcggtcaaac tcaccctcat cggtgtagat aacagacaat gcgctgcacg gtgcatcggt
    25981 cgaggcgata gtcggtgcga tcgctaccgg aacacccatg aaatgtgcca gtgctttggc
    26041 agtatcgagg gtttttccgc caccgatacc gagaattgcg ccacactgcg cagtctccgc
    26101 gatgccacgc agacggtcga tctcattttg cgaacattca ccgccaaacg gcgtaatttc
    26161 tactaccagt ccagcatctt taaagctttt ctcgacagtg gattgagcaa aaactaaaac
    26221 aaatttgtca cccaccacta accagcgttc tgccagcggc ttcaggtatt cgcccagacg
    26281 attaatcaca tcagcgccct ggatgtattt acccggtgat tgaataatgc ggtccataat
    26341 tgctccttta gagatgagta gtgccaaatg cggcattcca gtcgtgttcg aacttctcta
    26401 tagctgactc taccgcaggg gtgttgagca tttgttgcgc tacatctaag ggcagggtga
    26461 tggattcaca tcctgccagt aaacagtcca gcgcctgacg cggcgtttta aagctggctg
    26521 ccagcaccat gctttctggc gcgtgcattt ctaacagcgt ttgcagctcc tgaaccgtac
    26581 gaatgccgtc cccgccctgg gcatctacgc ggttaacata cggagcaacg tattttgccc
    26641 ctgccagtgc ggctaataac ccttctgcgg cgctatatac agcggtgcca agtgtagtaa
    26701 taccctattt tttcagtatt ttaattgctg ccagaccttc ggaagtcacc gggattttca
    26761 ccacaatacc cggaatagcg tcgcgcaggc gcttcgcttc ttccaccatc ccctgcgcgt
    26821 cgcggctcat ggtctgagca aacagaatgc cctcatcacc aatcgctttt tgcagacgcg
    26881 gcagcacttc ccagatggac tccttgctgg cagcgataat gctcgggtta gttgtcaccc
    26941 cggcaatcgg gaatatgcgt gccagacgtt cgacttctgc gacgttagcg gtgtccagat
    27001 acagttccat gatgtttcct tttacagttc cagttcatgt tgcagcaggc tggcgatagc
    27061 gtcggcagag gcggcgttaa ccagcgcgtt acggaattct tcgtgcatga tgcggcgagc
    27121 gaggcgcgag aaaatgcgca tgtgctgatc gcccgcagcg tgtttgttca gggttaacat
    27181 aatgatgaat tgcgcttcat catcgcccca gcgcaccggc gcttgcagac gcgccacgct
    27241 gatggtggat tgctcaatgt gttctgattt gctgtgtgga atggcaaaac taaagcccag
    27301 accggtagag aaaacggcct cgcgcgccca taagtcggct tccagtttac gcggatagcg
    27361 gcagcggccc gccagcagca ggttatcggt catgcctttg agcacttctt ctttgctgcg
    27421 ccagtcactt tccagcgtga tgcactcggc ggtgaccagc ggtgcgtcct gttgggtcat
    27481 gcggaattgc gccagcaggt gttccacttc cagcgaagta cggcaggcca ttgcctggtt
    27541 gagcaacttg cggcactcac ggctatcaag ttgcgccatc cgcgctttcg ccgccggaat
    27601 tgatggtgcg ctcatgctga gttcatccag ccctaagccg accagcaacg gcagcacgga
    27661 acctttcgct cccagctcac cgcacagacc aatccattta ccctggcgat gcaccgcctg
    27721 cacgtcgtaa tcgagcgccc gcaagaatgc cggattcagg ctgttgtagt gacgagtaac
    27781 cttagcgtta tcgcgatcca ctgccagcag atattgcgtc aggtcattac taccaatact
    27841 aaagaaatca atctcttcgc agcattgatc gatgatgaac atcaccgacg gcacttccag
    27901 cataatgccg agctggatct tctcatcaaa cggaatgtgt tcgttacgta gttgctgttt
    27961 ggcttctgcc agtttttctt tcacccataa gatctcttcc attgaggaga tcatcgggat
    28021 catgattttc aggctgccgt gagcggaggc gcgcaggatc gagcgtagtt gtgtggtaaa
    28081 taacgacgcg tattcttcat aaatacgcac ggcgcgataa ccgaggaacg ggtttgcctc
    28141 tgcgggaatg tttagataat caacgggttt gtcgccgcca atgtccatag tgcgcacaat
    28201 aatgctgcgt ccgttggcag actccagcgc ctgacaaaaa atgttgtaca gctcgctttc
    28261 gcccggtgca ctggtgcgat ccatatagag catttcagtg cggaacaaac caacgccttc
    28321 cgcaccattg ccgaatgcgg cctgcgcttc cacggagtga gcgatgttag cggcaatttc
    28381 aatgcggata ccgtcagcgg tacgggcttg ttgggtcagc tagacacgct gttgctcacg
    28441 cagggcgtcc tgtacgcggg cttcttgctg ataataacga gctacggctt ctcctggctc
    28501 aaccacaatc gccccggcgt tgccgtcgat ataaatcgtt tgatgctgcc acggagtgag
    28561 ggcatcaata tccacaccaa ccagcgtcgg aatgttgaac gaacgggcaa ggatcaccgt
    28621 atgtgaggtg gtgccgccgc ttttcagtaa caatcctttg aggtgatttt tatccagttc
    28681 gaggaactgg ctgggggtca gttcatcagc catacaaatg gcggactgcg tcagtttgcc
    28741 cggtgccggg aagcgttgct caccgtagat ttgctggagt aactggaagc agacgtcgcg
    28801 tacgtccagg gcacgttctt gcaggtatct gctgctggaa cgggcaaact cttcgcaaaa
    28861 gtgattcgca ctggcgacaa tcgcttcggc gcagcttaat ccggcgctga cgcctgccag
    28921 taaatgttcg tgcagggaag tatcgccagc cagggatcgg tgcgcttcca gaatcgcgct
    28981 ggtagcaccg tcgctatcca gcagacgaaa ctcaatgttt ttcagtacca gcgtcaggcc
    29041 gttttccagt gcggattgct cggcgtcaac gtctttggct gcgggaagat tactcagcgc
    29101 attgagatct aaagaagaga tcggcgtcag aatgccgcct gcactaccgc tgcacacggt
    29161 gcgggcgcgg ataatttgcg gattcagatt ggtcagtgaa accggcagtg gttccagttc
    29221 gtcagattta acttccgcca gcggcgcatc gcagtggggg aattcatcgc gcagccattg
    29281 gcttaaacgc tggtgccctt cctgttcgtc ggccccggaa atcagtagct ggcagttatc
    29341 acccaccagc gtatcggtgc caatcagcgc cagagcgctt ttggcgttgc ctttacggtc
    29401 agtacgcagg ttatgccact caatttgaga tgaaaaagta ttacacagcg tttcaacgtg
    29461 gcttgccgga cgcgcatgta cgccgttagg tagctcacaa ataaattcca caatcagggc
    29521 cattgccttt ctcccggtac ggttttctgt ttacaggata agagcgcacg gtaacggcct
    29581 gccatgtgac aaatctgcca aaagctggac aaatgtaatg taaccgtcaa tttgcgacgc
    29641 gtctcacaag acgccgtttt gcggcatgct tccggattat cgcaagttat gaggcggatc
    29701 gcatttttgt actgatatta caaaagtcca gtaaatggcc tttttatcca ctgtttgctc
    29761 cgcctgcgat tgcctattgt ttcctccatc aaaaatatgg acatgggctt ttgcaagccc
    29821 gggagcagct tatgaatgag ttggtgcaga tcctgaaaaa tacccgtcag catttaatga
    29881 cgggcgtttc acacatgatt cccttcgtgg tatcgggcgg tattttgctg gcggtttccg
    29941 tcatgttgta tggcaaaggc gcagtgccgg atgccgtagc cgatccgaat ttgaaaaaac
    30001 tgtttgatat cggcgttgca ggtttgacgc tgatggtgcc tttcctcgcc gcttacattg
    30061 gttactccat tgcagagcgt tctgcgctgg ctccgtgcgc tatcggtgcc tgggttggta
    30121 acagctttgg tgcgggcttc tttggtgcgc tgatcgccgg gattatcggc ggcatcgtgg
    30181 tgcattacct gaagaaaatt ccggtgcata aagttctgcg ctcggtgatg cccatcttca
    30241 ttattcctat catcggcaca ctgattaccg caggcatcat gatgtggggc ttgggcgagc
    30301 ctgtaggggc gttgaccaac agcctgactc agtggcttca ggggatgcag cagggcagca
    30361 ttgttatgct ggcggtgatc atggggctga tgctggcgtt cgatatgggc ggtccggtta
    30421 acaaagtggc ctatgccttc atgctgattt gcgttgctca gggtgtttat accgtggtgg
    30481 ccattgctgc cgtgggtatt tgtatcccgc cgctggggat ggggctggcg acgctgattg
    30541 gtcgtaaaaa tttctccgca gaagagcgcg aaaccggcaa agcggcactg gtgatgggct
    30601 gtgttggcgt tactgaaggg gcgattcctt tcgccgctgc cgatccgctg cgtgtcattc
    30661 cttccattat ggtcggttca gtttgtggtg cagtaactgc ggcgctggtc ggtgcgcagt
    30721 gctatgcagg ctggggtggt ctgattgtgc tgccggtggt tgaaggtaag ttgggttata
    30781 tcgcagcagt ggctgtcgga gcagtggtga cggctgtttg tgtgaacgtg ctgaaaagtc
    30841 tggcgcgtaa aaatgggtct tcgactgatg aaaaagaaga cgacctggat ttggattttg
    30901 aaattaatta attgaggaac gagccatgac gaaaattatt gcagtaaccg catgtccttc
    30961 aggtgttgcc catacctata tggcagcaga ggcgctggaa agtgccgcga aagcgaaagg
    31021 ctgggaagtg aaagtagaaa cccaggggtc aattggtctg gaaaatgaac tgactgcgga
    31081 agacgtggcg agcgctgata tggtgattct gaccaaagat atcggcatca agtttgaaga
    31141 gcgttttgct ggcaagacca tcgtgcgcgt caacatcagc gatgcagtaa aacgtgctga
    31201 cgccattatg agcaaaattg aagctcacct ggcgcaaacc gcttaagttc ttctcccgct
    31261 cgcaagggcg ggttcgcttt cccacaggag ttcctcatga cgaatcgtat ctctcgcctc
    31321 aaaactgcac tgtttgccaa tacccgtgaa atctcgctgg agcgggcgct gctttatacc
    31381 gccagccatc ggcaaaccga aggcgaaccg gtgatattgc gccgggcgaa agcaacagcg
    31441 tatatccttg aacatgttga aatttcgatt cgtgatgaag aactgattgc cggtaaccgc
    31501 accgtaaaac cgcgcgccgg gattatgtcg ccggaaatgg acccttactg gctgctgaaa
    31561 gagctggatc aattcccgac gcgtccgcag gaccgctttg ctatcagcga agaagataaa
    31621 cgtatctacc gtgaagagtt gttcccgtac tgggaaaaac gttcgatgaa agatttcatc
    31681 aacgggcaga tgacagatga agtaaaagcc gcgaccaaca cgcagatttt cagcatcaac
    31741 cagacggata aaggccaggg gcacattatt attgattacc cacgcctgtt gaatcacggt
    31801 ctgggtgagc tggtggcaca gatgcagcaa cattgtcagc aacagccgga gaatcacttt
    31861 tatcaggccg cgttgttact gctggaagcc tcgcagaaac acattttgcg ttacgccgaa
    31921 ctggcggaaa cgatggcggc aaactgcaca gatgcccagc gtcgcgaaga gctgctgact
    31981 attgcggaga tctcccgcca taacgcgcaa cataagccgc agacgttctg gcaggcgtgc
    32041 cagttattct ggtacatgaa catcattctg caatacgaat ccaacgccag ttcgctgtct
    32101 ttagggcgct tcgaccagta tatgttgccg ttctatcagg catctttaac ccagggcgaa
    32161 gatccggcgt tcctgaaaga actgctcgaa tctttatggg tgaaatgcaa cgacatcgtg
    32221 ctgttgcgct ccaccagtag cgcgcgttat ttcgccggtt tcccgaccgg ctataccgca
    32281 ctgctcggcg ggttaaccga gaacggacgt agcgcggtga acgtgctttc gttcctttgc
    32341 cttgacgcct atcaaagcgt gcaattaccg caaccgaacc tcggcgtgcg cactaacgcc
    32401 ttgatcgaca cgccgttcct gatgaaaacc gccgaaacca ttcgcctcgg caccggtatt
    32461 ccgcaaatct ttaacgatga agtggtggtg ccagcgttcc tcaaccgtgg cgtttcgctg
    32521 gaagatgcgc gcgactattc cgtagtgggc tgtgtggaat tatctattcc cggcagaacc
    32581 tacggcttgc atgacatcgc gatgtttaat ctgctgaaag tgatggaaat ctgcctgcat
    32641 gaaaatgaag gcaatgctgc gctgacttat gaaggtttac tggagcagat ccgcgccaag
    32701 atcagccact acatcaccct gatggttgag ggcagcaata tttgtgatat cggccatcgc
    32761 gactgggcac ctgtaccgct gctctcgtct tttatcagcg attgtctgga aaaaggtcgc
    32821 gatattaccg atggcggcgc gcgttataac ttctccggcg tacagggaat tggtatcgcc
    32881 aacctgagcg attctctcca tgcgttgaaa gggatggttt ttgagcaaca gcgtttaagt
    32941 tttgacgaat tgctgtcggt attaaaagcc aactttgcaa cgccagaagg cgaaaaagtc
    33001 cgcgctcgct taattaaccg ctttgagaaa tacggtaacg atatcgacga ggtggataac
    33061 attagcgccg aactgttgcg ccactactgc aaagaagtgg aaaaatacca gaacccgcgc
    33121 ggcggttact tcacgccggg atcgtatacc gtttctgctc acgttccgtt gggatcggtg
    33181 gttggcgcga cgccagacgg tcgttttgcc ggggaacaac tggcagacgg cggcttgtca
    33241 cctatgctgg gtcaggacgc acaagggcca acggcggtac tgaagtcagt cagtaagctc
    33301 gataatacgc tgctgtctaa cggtacgttg ctgaacgtga aattcactcc ggcgaccctg
    33361 gaaggtgaag cgggattacg caaactggcc gacttcttac gggtgtttac ccagcttaag
    33421 ttgcagcata ttcagtttaa cgtggtgaac gccgacacgt tgcgggaagc gcaacagcgc
    33481 ccacaagatt atgccgggct ggtggtgcgc gttgccggat acagcgcctt ctttgtcgaa
    33541 ctgtcgaagg agatccagga tgacatcatc cgccggacag cgcatcagct gtaacgttgt
    33601 ggaaacgcgc cgcgatgatg tggcgcgcat tttcaacatt cagcgttatt cactgaatga
    33661 cggcgagggc attcgtacgg tggtcttttt taaaggctgt ccgcatcttt gcccgtggtg
    33721 tgctaatccg gagtcgatct ccggcaaaat ccagacggta cgcagagagg cgaaatgtct
    33781 gcactgtgcg aaatgtttgc gtgatgcgga tgaatgcccc tccggggcgt ttgaacggat
    33841 tggtcgcgat atcagccttg acgctctgga acgggaagtg atgaaagatg acattttctt
    33901 tcgcacgtcc ggcggcggcg tcacgctttc tggcggcgaa gtgttaatgc aggcggagtt
    33961 tgctacccgt tttttacagc gactgcggct gtggggtgtc tcatgcgcca ttgaaactgc
    34021 cggagacgcg ccagcaagca agctgttacc gctggcgaaa ttgtgcgatg aagtgctgtt
    34081 cgatttaaaa attatggacg cgactcaggc gcgggatgtg gtgaagatga acctgccacg
    34141 cgtgctggag aatctgcgtt tgctggtgag tgagggtgtc aacgtgatcc cgcgtttacc
    34201 gttgatccct ggtttcacgc tcagccggga gaatatgcag caggcgctgg atgtactgat
    34261 cccgctgaaa atcaggcaga tccatctgtt accgtttcat cagtacggcg aaccgaaata
    34321 ccgcctgctg gggaaaacat ggtcgatgaa agaggtgcct gcgccgtcgt cagccgatgt
    34381 gacaacgatg cgcgaaatgg cagaacgggc cggatttcag gttaccgtgg gaggttaaaa
    34441 tggcatacct ggtggcagta accgcctgcg taagcggcgt ggcgcatact tatatggcgg
    34501 cggaacggct ggaaaagttg tgccagttag agaagtgggg cgtcagcatt gaaactcagg
    34561 gcgcgctggg aacggagaat cgtttagcgg acgaggatat ccgtcgggcg gatgttgctc
    34621 tgttgattac ggatatcgag cttgccggtg ccgagcgatt tgaacattgc cgctatgtgc
    34681 aatgcagcat ctacgcattc ctgcgtgagc cgcagcgggt aatgagcgcg gtgcgcaaag
    34741 tgctttctgc gccgcagcaa acccatctta ttctggagta gtcggttttt ctgtcagttg
    34801 gctgtggtac tgccggcgat attccgacgg cgagtgttcg gtgtttttac gaaacagacg
    34861 gcagaagtag ttgctgtcga caaagccgca ggcgtgcgcc acttctttta ccttcaggtc
    34921 atagcctttc aataacgtct tagcgtgctc cagtcgcgtg tgattcaggt attcgttaaa
    34981 accaatggcc cccgtttttt gaaacaggtg cgagagatag tttggtgaaa tatagaatgc
    35041 ctgtgcgaca gattcgcggg taagcgcgga ggcatagcgt tcgtcgatat aatcgcgaat
    35101 agcttcaaat agtgcctggc tgcgtgaggc ggtctggatt tggctgccga gcaaatcgcg
    35161 gcagtggcta agcaggctgg ctacgataag ccttgccgtt tgctgctcct gcggctgcat
    35221 ttgcatttca ttgagcgttt gtagcagaaa agaacctatg cgtgggccgc gtcgggcgac
    35281 gtgttgcttc gccagatttt gatattgttt gccatcccat tgcacgacgc tgaagccgag
    35341 ttgctgtttg ccaaacagca cgctaaaggt ggtagcgggt gttttccatt gtgggaaatt
    35401 ccagccacca gcggcaacgt acagcacatc gccgggaact aacgttgctc cagcgccggt
    35461 atcaacaaac tcaccttcca gcacaatttc cagccgtgga aaatcgacct gatacgcaag
    35521 atcgggcacc gggccgttac tgctggcaaa gtaaatctgg cgcagggaca gcgggccgtt
    35581 gatcaggcgg gagagcagat agctgacgtc gtgatacatg ttattccctt aggtatgtag
    35641 acgggaaaac agtaactggc attattaaaa aaggccacct gaaaagtgtg gcctgagggg
    35701 agttcgatgt aaacattgcg gcagtatcgc atccggcaaa tagcgccaga cgcgatacaa
    35761 aggtcatcta ctgattaccc acctgatcgc catacggcag agtgtcgtaa tcgatcagcg
    35821 cgtttttctt gtacgggtta ccaatccagc gggtagtttc tttgaactgc ggattcacca
    35881 ctgaacgggt tggatcgtaa ccgtcgtaag ataagcccgc caaatctgac caggtgtgga
    35941 tcagttccgc caggctgtat ttacgatcga catcctgtga gaaatcacgg ggatgagtcg
    36001 cttgccattt ttccgacgtc cacagcagga acggaatggt gtacatgtgg cgcgtcgggt
    36061 tgtcttcgtt acgcccctga gttttgtgcg gcggcgtgtc gtaaacctct tcaccgtggt
    36121 cagagaagta aaccaggaat ccgttcgggt ctgccgcttt aaagtcttta atcaggctgg
    36181 caaccacatg atcgttatac aggttagcgt tgtcataatc gttatatgat tccagctctt
    36241 ctgcgcttaa tcctggcgga acatgatcgg tattgccatc aaacttgccc tgattttccg
    36301 ggtagcggta tttgtatttg atatgcgtac ccagcagatg aacgatgatc agtttcttcg
    36361 gcgcagggtc attcagcaca tcctggaacg gcttcagcac gttggtgtcg tattcacgcg
    36421 cactctgcgt acgttgctgg ttcatgtagt actgcttgtc ggtctggcgc gaaaataccg
    36481 tcagcatggt attgcgggcg gtcatcgtct gctggttggt gatccagaag gttttataac
    36541 ccgcctgttt catcatgttc atcagcgacg gctgcgtcag atacagatcc gggttctttt
    36601 cattggcaaa ggtcagcgcc tgttgcagga tttcaatggt gtacggacga gacgtaacta
    36661 cgttattaaa cacggtcaga ttcggatcgg ttttatgcag cgcatccagc tccggcgtgg
    36721 tttcacgcgg ataaccgtac agactcatgc gtccgcgctg ggtcgactcg ccaatcacca
    36781 gcactaaagt gcgcggttcg ttacccgatt catctttgaa attagccagt ggcggcaagg
    36841 cattattttc attcagtaac tttgttagcg agtttagttg ctgacgatac tgataatagc
    36901 cggtcaggaa ttgccacggt gcggcaggct ccatacgcga ggctaggtta tccaacgttt
    36961 tctcaaacgg cttgtttttg ataaacgtat tcatggcgat cggatgcaga atcaagccat
    37021 aaagcagggc aaaagagaca acataacgcc acggctttgg aatatagacc gggcgcaggc
    37081 gtgtccacag cagaactgcc accgccgtat aggccagcgc gataagcaca attttcaggc
    37141 tgaaatactg gcttaaatac tcgctggctt cgttggtgtt ggtttcgaac atcacaaaca
    37201 gaacgctctg cgagaactcc tgcccgtaga tgacgtagta gcacagcgcc gccagagagg
    37261 ccgtccatag caccacgccg atcactgcgg caataatttt aatccgcttc ggaaagagga
    37321 ataccgggat caaccacagc gagctgaata acagcgagtc gcgaatgccg ttggtgccac
    37381 tataaccact gatgtaaata atggcctgta gcagagtaga gaaaaaccaa aagtagagca
    37441 gtgcccaacc cagggctttc cagctagaaa ggggtttagc ctggacttct gtggaatgca
    37501 tagtaagaac ctgtcttgaa aaaaatatcg ccgaatataa cgacaattcc ttaaggatat
    37561 ctgaaggtat attcagaatt tgaataaaat gcagacagaa atatattgaa aacgagggtg
    37621 ttagaacaga agtatttcag aaaaccctcg cgcaaaagca cgagggtttg cagaagagga
    37681 agattagccg gtattacgca tacctgccgc aatcccggca atagtgacca ttaacgcttg
    37741 ttcgacgcga ggatccggtt cctggccttc tttttctgcc tggcgggagc ggtgcagcaa
    37801 ctcggcctgc aatacgttca gcgggtcggt gtaaatattc cgtagctgaa tagactctgc
    37861 aatccacggc agatcggcca tcagatggga atcgttggca atcgccagca ccactttgat
    37921 gtcttcttct tgcaggttgc gtaactcttt acctaacggc cacagtgctt tgtctaccag
    37981 acgttgatca tagtattccg ccaaccacag gtctgctttg gcgaagacca tctccagcat
    38041 gccgagacgc gtcgagaaga atggccaatc gcggcacatg gcttccagct cgctctgttt
    38101 gccatcttcg accacttttt gcagcgccgt acctgcaccc agccaggcgg ggagcatcag
    38161 gcggttttgc gtccaggcaa aaatccacgg tatggcgcgc agcgactcga cgccgccggt
    38221 tgggcgacgt ttcgccggac gtgaacccaa cggcagtttg cccaattctt gttccggcgt
    38281 agcggagcgg aagtaaggca caaaatcttt gttttcacgt acgtagccac ggtagagatc
    38341 gcaggagatg actgacagtt catccataat gcgacgccag ctctctttcg gctcaggtgg
    38401 tggcagcagg ttggcttcca gaatcgcccc ggtgtaaagc gacaggctgc tgacggtgat
    38461 ttctggcaga ccatatttaa agcggatcat ctcgccctgt tcagtcacgc gcaggccacc
    38521 tttcaggctt cctggcggtt gtgacagcag cgccgcatgg gcaggtgcgc cgccgcgacc
    38581 aatggaaccg ccgcgaccgt ggaacaacgt cagctcaata cccgcttttt cgcaggtttt
    38641 gattaatgca tcctgtgcct gatattgcgc ccaggaagct gccatcacgc ccgcatcttt
    38701 tgctgagtcg gaatagccaa tcatcaccat ctgtttgccc tgaatcaggc cgcgatacca
    38761 gtcgatattg agcagctggg tcatgacatc gttggcgttg ttcaggtcat cgagggtttc
    38821 aaacagcgga gcaaccggca tcgcaaaccc gatacccgct tctttcagca gcaggtggac
    38881 agccagtacg tcggacggcg ttttcgccat cgagatcacg taggcggcaa tggagccttg
    38941 cggcgcttcg gcaatcacct ggcaggtatc gagcacttcg cgcgtttcgg cgcttggttg
    39001 ccagttgcgc ggcagaagcg gacgtttgga gttcagttcg cggatcagga acgcctgttt
    39061 gtcggcctct gaccagcttt cgtagtcgcc gataccgagg tagcgggtca gctcgcccag
    39121 cgcttcggta tggcgggtgc tctcctgacg gatatcaata cggaccagcg gtacgccgaa
    39181 acatttcacg cggcgcaggg tgtcgagcag atcgccgttg gcgataatac ccatgccaca
    39241 cgcctgaagt gactggtagc aagcgtagag cggttcccac agttcttcgt tttgtgtcag
    39301 cagcccttct ggttttggca gttcttcgcc tttcaggcgc gcttccagcc atgcctgtgt
    39361 cgccatcagg cgagaacgca ggtttttcat cagatagcga tacggttctg cggcaccttc
    39421 ttcgccaacc agcgccagca gttcaggggt cgcttcaacc atcgacagtt cagaaaccag
    39481 aacctgaata tctttcagga acagatcggt ggctttccag cggctgagca gcaggacgtg
    39541 gcgggtgata tcggcagtga cgttcgggtt gccgtcgcgg tcaccgccca tccacgaggt
    39601 aaaacgtacc ggaacaaatt cgacgggcag tttgtagccg agattctctt ccagttgttc
    39661 gttcagttcg cgcaggtaat tcggtacgcc ttgccacagg ctgttttcta ctacggcaaa
    39721 gccccatttg gcttcatcta ccgggcttgg acgcagctta cggatttcat cggtatgcca
    39781 tgactgggcg atcaactggc gcaggcgacg catcagctgg ttgcgttcgt agtcagcgat
    39841 atctttgtta tcgagctgtt ttaaacaggc gttcacttcc accattttgt ggatcagtgt
    39901 acgacgggta atttcggttg ggtgagccgt gaggaccagt tccagcgaca gcgattccac
    39961 tgcttttttg atggtgtctt cgctcagttc cggctggttt ttcagtttac gcagggtgcg
    40021 ggcgatcact tccgggttgc tggcagcttc gcctttcggc gaaatgctgt ggtattgctc
    40081 ggcggtgttg gccaggttca ggaactgact aaacgcacgc gcaacgggca gcagctcgtc
    40141 gttcgacaaa ttttgtaagg tggtgagcaa ctcctggcgg ttagcatcat tgccagcgcg
    40201 tgaagatttc gacaacttac ggatagtttc tacgcgttca agaatgtgtt ctcccaacgc
    40261 atccttgatg gtttctccca gcactttgcc gagcatactg acattactac gcaatgcgga
    40321 atattgttcg ttcatattac cccagacacc ccatcttatc gtttgatagc cctgtatcct
    40381 tcacgtcgca ttggcgcgaa tatgctcggg ctttgctttt cgtcgtcttt tataaagcca
    40441 cgtaaaagcg gtgacgtcaa atgctgcgaa atcgcttcag caaacgaata aatagcagga
    40501 atttacgcca ttaaattcac gacgctttaa ataagcgtaa cttatggaaa tgttaaaaaa
    40561 tcgccataca taacaccaaa ggtgtaggtc ggataagatg cgcaagcatc gcatccgaca
    40621 ttattgcggc accggagttt ggcaacggtg ccggatgcgg cgcgagcgcc ttatccggcc
    40681 tacgttttaa tgccagcaaa aatggtgaat tacctggata atcaactcac gggtgggttt
    40741 aataaaccgc gtctccagat attcatcagg ctgatgagct tgattgatgg aacccggacc
    40801 gagcaccagc gtcgggcata acgtttgaat aaacggcgct tcggtacagt aattcaccac
    40861 ttcggttttt gctccgagca atttctcaac cacttcaacc agttgatgat tcggtgggca
    40921 ttcatagcca gggatcggcg gatgcagctc gtcgaccgtc agacgacccg gccagcgttc
    40981 gctcaccgga gccaatgcat cgttgagcaa accattaagt tcattgagtg tcatgccagg
    41041 ctgcggacga atatccatat gcaactcaca gcaagcgcaa atacggttag aagcgtcgcc
    41101 accgtgaata tgcccgaggt tgagcgtagg gtatggcacg gtaaatgctt cgtagtgata
    41161 acgttctttc aggttatcgc gcaattgcaa aatatgcccg atggcgtcgt gcattagttc
    41221 gatagcgtta attccgcgtg ctggatcgct ggagtgcccc gactggccct gaatacggat
    41281 ggcgttagag atatgacctt tatgtgcgcg taccggttgt agtgacgtcg gttcgccaat
    41341 gatggcgcaa tccgggcgca gggcggtagt ttcggcaaaa taacgcgctc cggccatact
    41401 ggtttcttca tcagcagtcg ccagaatgta gagcggtttt ttcagtttcg tgacgtcgac
    41461 atcgcgtagc gcatcaagga taaacgcaaa aaagcctttc atgtcggcgg tgcctaagcc
    41521 gtaaagcttg ccgtcatgct ccgtcagtgt aaacggatcg cgcgtccagc gaccgtcatc
    41581 aaatggcacc gtatcggtat gccccgccag caacaagccg ccagccccct gtccgtaact
    41641 ggccagcata ttaaatttgt tgcgagttcc tggaacaggc tgcacttcca cattgaagcc
    41701 caaatcttta aaccagtccg ccagcagagt gattaaatct gcattgcttt gatcgagtgc
    41761 ctcttccgta gcgcttattg aaggtgtggc aatcagagcg cggtaaatct cgataaatgg
    41821 cggtaatttg tttttcattg ttgacacacc tctggtcatg atagtatcaa tattcatgca
    41881 gtatttatga ataaaaatac actaacgttg agcgtaataa aacccaccag ccgtaaggtg
    41941 aatgttttac gtttaacctg gcaaccagac ataagaaggt gaatagcccc gatgttgaat
    42001 acgctgattg tgggtgccag cggctacgct ggcgcagagc tagtgaccta tgtaaatcgc
    42061 catccgcata tgaacataac cgctttgact gtttcagcgc aaagcaatga tgcgggaaag
    42121 ttaatctccg atttgcatcc gcagctaaaa ggcatcgttg agctgccgtt gcagccgatg
    42181 tcggatatca gcgagtttag cccaggggtg gacgtagtgt ttctcgccac cgcccatgaa
    42241 gttagccacg atttagcgcc gcagtttctt gaagcgggct gcgtggtgtt cgacctttcc
    42301 ggcgcgtttc gtgttaacga cgccaccttc tatgaaaaat attacggctt tacccatcaa
    42361 tacccggaac tgctggaaca ggcagcctac ggtctggcgg agtggtgcgg taataaatta
    42421 aaagaagcga atttgattgc ggtgccaggc tgttatccga cggcggcaca gctggcgctg
    42481 aaaccgttga ttgatgccga tcttcttgac ctcaatcagt ggccggtgat caacgccacc
    42541 agcggcgtga gcggtgcagg acgtaaggca gcgatttcaa atagtttttg tgaagttagc
    42601 ctgcaaccgt atggcgtctt tactcatcgc catcaaccag agatcgccac acacctcggt
    42661 gctgacgtta tcttcacccc acatctgggc aatttcccgc gcggcattct cgaaaccatt
    42721 acctgccgcc tgaaatcggg tgtgacccag gcgcaagtcg cgcaagcgtt acagcaggcg
    42781 tatgcccata aaccgctggt gcggctgtat gacaaaggcg ttccggcgct gaaaaatgtc
    42841 gttgggctgc cattttgcga tatcgggttt gccgttcagg gcgagcatct gattattgtg
    42901 gcgaccgaag acaacttact gaaaggcgcg gcggcacaag cggtacagtg cgccaatatt
    42961 cgtttcggct atgcggaaac gcagtctctt atttaagggt gcaatgatga atccattaat
    43021 tatcaaactg ggcggcgtac tgctggatag tgaagaggcg ctggaacgtc tgtttagcgc
    43081 attggtgaat tatcgtgagt cacatcagcg tccgctggtg attgtgcacg gcggcggttg
    43141 cgtggtggat gagctgatga aagggctgaa tctgccggtg aaaaagaaaa acggcctgcg
    43201 ggtgacgcct gctgatcaga tagacattat caccggagca ctggcgggaa cggcaaataa
    43261 aaccctgttg gcatgggcga agaaacatca gattgcggcc gtaggtttgt ttctcggtga
    43321 cggcgacagc gtcaaagtga cccagcttga tgaagagtta ggtcatgttg gactggcgca
    43381 gccaggttcg cctaagctta tcaactcctt gctggagaac ggttatctgc cggtggtcag
    43441 ctccattggc gtaacagacg aagggcaact gatgaacgtc aatgccgacc aggcggcaac
    43501 ggcgctggcg gcaacgctgg gcgcggatct gattttgctc tccgacgtca gcggcattct
    43561 cgacggcaaa gggcaacgca ttgccgaaat gaccgccgcg aaagcagaac aactgattga
    43621 gcagggcatt attactgacg gcatgatagt gaaagtgaac gcggcgctgg atgcggcccg
    43681 cacgctgggc cgtccggtag atatcgcttc ttggcgttat gcggagcagc ttccggcact
    43741 gtttaacggt atgccgatgg gtacgcggat tttagcttaa gttttgttag ccggaggcgc
    43801 agctttccgg cattgaattt caaaataagg aaacagagtt atggcacttt ggggcgggcg
    43861 ttttacccag gcagcagatc aacggttcaa acaattcaac gactcactgc gctttgatta
    43921 ccgtctggcg gagcaggata ttgttggctc tgtggcctgg tccaaagcct tggtaacggt
    43981 cggcgtgtta accgcagaag agcaggcgca actggaagag gcgctgaacg tgttgctgga
    44041 agatgttcgc gccaggccac aacaaatcct tgaaagcgac gccgaagata tccatagctg
    44101 ggtggaaggc aaactgatcg acaaagtggg ccagttaggc aaaaagctgc ataccgggcg
    44161 tagccgtaat gatcaggtag cgactgacct gaaactgtgg tgcaaagata ccgttagcga
    44221 gttactgacg gctaaccggc agctgcaatc ggcgctggtg gaaaccgcac aaaacaatca
    44281 ggacgcggta atgccaggtt acactcacct gcaacgcgcc cagccggtga cgttcgcgca
    44341 ctggtgcctg gcctatgttg agatgctggc gcgtgatgaa agccgtttgc aggatgcgct
    44401 taagcgtctg gatgtcagcc cattaggctg tggcgcgctg gcgggaacgg cctatgaaat
    44461 cgaccgtgaa cagttagcag gctggctggg ctttgcttcg gcgacccgta acagtctgga
    44521 cagcgtttct gaccgtgacc atgtgttgga actgctttct gctgccgcta tcggcatggt
    44581 gcatctgtcg cgttttgctg aagatctgat tttctttaac accggcgaag cggggtttgt
    44641 ggagctttcg gaccgcgtga cttccggttc atcattaatg ccgcagaaga aaaacccgga
    44701 tgcgctggag ctgattcgcg gtaaatgcgg ccgggtgcag ggtgcgttaa ccggcatgat
    44761 gatgacgctg aaaggtttgc cgctggctta caacaaagat atgcaggaag acaaagaagg
    44821 tctgttcgac gcgctcgata cctggctgga ctgcctgcat atggcggcgc tggtactgga
    44881 cggcattcag gtgaaacgtc cgcgttgtca ggaagcggcg cagcaggggt atgcgaacgc
    44941 cactgaactg gcggattacc tggtggcgaa aggcgtacct ttccgcgagg cgcaccatat
    45001 tgtgggtgaa gcggtggtgg aagccattcg tcagggcaaa ccgctggaag atctgccact
    45061 cagtgagttg cagaaattca gtcaggtgat tggcgaagat gtctatccga ttctgtcgct
    45121 acaatcgtgc ctcgaaaagc gtgcggcaaa aggcggcgtc tcaccgcagc aggtggcgca
    45181 ggcgattgct tttgcgcagg ctcggttaga gtaatttgtt tatattgctc ccctgaaaat
    45241 ggggagttta ccacgcctac aaagtgcgac acttcgtagg cggataaggc gtttacgccg
    45301 catcaggcag aaatttgtca gcattc
//
LOCUS       DAZOYF010000013        45006 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZOYF010000013 DAZOYF010000000
VERSION     DAZOYF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.013"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..1468
                     /locus_tag="ACHUOF_000724"
     CDS             <1..1468
                     /locus_tag="ACHUOF_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610023.1"
                     /translation="DGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHF
                     NNDKIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGND
                     TSYYQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKE
                     EEKHFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGK
                     TDGIAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFN
                     DRTGKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGD
                     SSAQFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAY
                     LQQAATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQN
                     GDGVERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            1521..3251
                     /locus_tag="ACHUOF_000725"
     CDS             1521..3251
                     /locus_tag="ACHUOF_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0610024.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(3311..3802)
                     /gene="yajQ"
                     /locus_tag="ACHUOF_000726"
     CDS             complement(3311..3802)
                     /gene="yajQ"
                     /locus_tag="ACHUOF_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR0610025.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            3970..4881
                     /gene="panE"
                     /locus_tag="ACHUOF_000727"
     CDS             3970..4881
                     /gene="panE"
                     /locus_tag="ACHUOF_000727"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR0610026.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            4844..5434
                     /gene="yajL"
                     /locus_tag="ACHUOF_000728"
     CDS             4844..5434
                     /gene="yajL"
                     /locus_tag="ACHUOF_000728"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR0610027.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(5488..6936)
                     /gene="thiI"
                     /locus_tag="ACHUOF_000729"
     CDS             complement(5488..6936)
                     /gene="thiI"
                     /locus_tag="ACHUOF_000729"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR0610028.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7142..7384
                     /gene="xseB"
                     /locus_tag="ACHUOF_000730"
     CDS             7142..7384
                     /gene="xseB"
                     /locus_tag="ACHUOF_000730"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR0610029.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            7384..8283
                     /gene="ispA"
                     /locus_tag="ACHUOF_000731"
     CDS             7384..8283
                     /gene="ispA"
                     /locus_tag="ACHUOF_000731"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR0610030.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            8308..10170
                     /gene="dxs"
                     /locus_tag="ACHUOF_000732"
     CDS             8308..10170
                     /gene="dxs"
                     /locus_tag="ACHUOF_000732"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR0610031.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            10225..11142
                     /gene="yajO"
                     /locus_tag="ACHUOF_000733"
     CDS             10225..11142
                     /gene="yajO"
                     /locus_tag="ACHUOF_000733"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR0610032.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(11253..11771)
                     /gene="pgpA"
                     /locus_tag="ACHUOF_000734"
     CDS             complement(11253..11771)
                     /gene="pgpA"
                     /locus_tag="ACHUOF_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR0610033.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(11749..12726)
                     /gene="thiL"
                     /locus_tag="ACHUOF_000735"
     CDS             complement(11749..12726)
                     /gene="thiL"
                     /locus_tag="ACHUOF_000735"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR0610034.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(12804..13223)
                     /gene="nusB"
                     /locus_tag="ACHUOF_000736"
     CDS             complement(12804..13223)
                     /gene="nusB"
                     /locus_tag="ACHUOF_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR0610035.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(13243..13713)
                     /gene="ribE"
                     /locus_tag="ACHUOF_000737"
     CDS             complement(13243..13713)
                     /gene="ribE"
                     /locus_tag="ACHUOF_000737"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR0610036.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(13802..14905)
                     /gene="ribD"
                     /locus_tag="ACHUOF_000738"
     CDS             complement(13802..14905)
                     /gene="ribD"
                     /locus_tag="ACHUOF_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR0610037.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(14909..15358)
                     /gene="nrdR"
                     /locus_tag="ACHUOF_000739"
     CDS             complement(14909..15358)
                     /gene="nrdR"
                     /locus_tag="ACHUOF_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR0610038.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            15509..16048
                     /locus_tag="ACHUOF_000740"
     CDS             15509..16048
                     /locus_tag="ACHUOF_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR0610039.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            16346..17230
                     /gene="tsx"
                     /locus_tag="ACHUOF_000741"
     CDS             16346..17230
                     /gene="tsx"
                     /locus_tag="ACHUOF_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR0610040.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(17407..17754)
                     /gene="yajD"
                     /locus_tag="ACHUOF_000742"
     CDS             complement(17407..17754)
                     /gene="yajD"
                     /locus_tag="ACHUOF_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR0610041.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(17883..18854)
                     /gene="secF"
                     /locus_tag="ACHUOF_000743"
     CDS             complement(17883..18854)
                     /gene="secF"
                     /locus_tag="ACHUOF_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR0610042.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(18865..20712)
                     /gene="secD"
                     /locus_tag="ACHUOF_000744"
     CDS             complement(18865..20712)
                     /gene="secD"
                     /locus_tag="ACHUOF_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR0610043.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(20740..21072)
                     /gene="yajC"
                     /locus_tag="ACHUOF_000745"
     CDS             complement(20740..21072)
                     /gene="yajC"
                     /locus_tag="ACHUOF_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR0610044.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21095..22222)
                     /gene="tgt"
                     /locus_tag="ACHUOF_000746"
     CDS             complement(21095..22222)
                     /gene="tgt"
                     /locus_tag="ACHUOF_000746"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR0610045.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLLEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(22277..23347)
                     /gene="queA"
                     /locus_tag="ACHUOF_000747"
     CDS             complement(22277..23347)
                     /gene="queA"
                     /locus_tag="ACHUOF_000747"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR0610046.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKSDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            23440..24013
                     /gene="acpH"
                     /locus_tag="ACHUOF_000748"
                     /pseudo
     CDS             23440..24013
                     /gene="acpH"
                     /locus_tag="ACHUOF_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24018..25835)
                     /gene="malZ"
                     /locus_tag="ACHUOF_000749"
     CDS             complement(24018..25835)
                     /gene="malZ"
                     /locus_tag="ACHUOF_000749"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR0610047.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(25991..27364)
                     /gene="proY"
                     /locus_tag="ACHUOF_000750"
     CDS             complement(25991..27364)
                     /gene="proY"
                     /locus_tag="ACHUOF_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR0610048.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(27440..28759)
                     /gene="brnQ"
                     /locus_tag="ACHUOF_000751"
     CDS             complement(27440..28759)
                     /gene="brnQ"
                     /locus_tag="ACHUOF_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR0610049.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29166..30461)
                     /gene="phoR"
                     /locus_tag="ACHUOF_000752"
     CDS             complement(29166..30461)
                     /gene="phoR"
                     /locus_tag="ACHUOF_000752"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR0610050.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(30519..31208)
                     /gene="phoB"
                     /locus_tag="ACHUOF_000753"
     CDS             complement(30519..31208)
                     /gene="phoB"
                     /locus_tag="ACHUOF_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR0610051.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            31398..32600
                     /gene="sbcD"
                     /locus_tag="ACHUOF_000754"
     CDS             31398..32600
                     /gene="sbcD"
                     /locus_tag="ACHUOF_000754"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR0610052.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            32597..35740
                     /gene="sbcC"
                     /locus_tag="ACHUOF_000755"
     CDS             32597..35740
                     /gene="sbcC"
                     /locus_tag="ACHUOF_000755"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR0610053.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            35866..37047
                     /gene="araJ"
                     /locus_tag="ACHUOF_000756"
     CDS             35866..37047
                     /gene="araJ"
                     /locus_tag="ACHUOF_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR0610054.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37190..38098)
                     /gene="mak"
                     /locus_tag="ACHUOF_000757"
     CDS             complement(37190..38098)
                     /gene="mak"
                     /locus_tag="ACHUOF_000757"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR0610055.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            38223..39134
                     /gene="rdgC"
                     /locus_tag="ACHUOF_000758"
     CDS             38223..39134
                     /gene="rdgC"
                     /locus_tag="ACHUOF_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR0610056.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39212..39667)
                     /locus_tag="ACHUOF_000759"
     CDS             complement(39212..39667)
                     /locus_tag="ACHUOF_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610057.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(39664..39999)
                     /locus_tag="ACHUOF_000760"
                     /pseudo
     CDS             complement(39664..39999)
                     /locus_tag="ACHUOF_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40154..40438)
                     /gene="ppnP"
                     /locus_tag="ACHUOF_000761"
     CDS             complement(40154..40438)
                     /gene="ppnP"
                     /locus_tag="ACHUOF_000761"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR0610058.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(40510..41187)
                     /locus_tag="ACHUOF_000762"
     CDS             complement(40510..41187)
                     /locus_tag="ACHUOF_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR0610059.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(41445..41636)
                     /gene="yaiA"
                     /locus_tag="ACHUOF_000763"
     CDS             complement(41445..41636)
                     /gene="yaiA"
                     /locus_tag="ACHUOF_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR0610060.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(41686..42210)
                     /gene="aroL"
                     /locus_tag="ACHUOF_000764"
     CDS             complement(41686..42210)
                     /gene="aroL"
                     /locus_tag="ACHUOF_000764"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR0610061.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(42393..42851)
                     /locus_tag="ACHUOF_000765"
     CDS             complement(42393..42851)
                     /locus_tag="ACHUOF_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR0610062.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            42971..43780
                     /gene="proC"
                     /locus_tag="ACHUOF_000766"
     CDS             42971..43780
                     /gene="proC"
                     /locus_tag="ACHUOF_000766"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR0610063.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(43797..44912)
                     /gene="adrA"
                     /locus_tag="ACHUOF_000767"
                     /gene_synonym="dgcC"
     CDS             complement(43797..44912)
                     /gene="adrA"
                     /locus_tag="ACHUOF_000767"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR0610064.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11620 a        11794 c        11087 g        10505 t
ORIGIN      
        1 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
       61 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      121 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      181 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
      241 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
      301 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
      361 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
      421 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
      481 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
      541 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
      601 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
      661 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
      721 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
      781 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
      841 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
      901 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
      961 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1021 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1081 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1141 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1201 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     1261 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     1321 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     1381 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     1441 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     1501 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     1561 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     1621 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     1681 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     1741 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     1801 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     1861 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     1921 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     1981 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2041 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2101 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2161 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     2221 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     2281 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     2341 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     2401 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     2461 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     2521 tggcgtttct gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag
     2581 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     2641 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     2701 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     2761 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     2821 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     2881 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     2941 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3001 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3061 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3121 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3181 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     3241 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     3301 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     3361 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     3421 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     3481 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     3541 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     3601 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     3661 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     3721 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     3781 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     3841 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     3901 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     3961 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4021 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4081 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4141 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4201 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     4261 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     4321 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     4381 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     4441 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     4501 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     4561 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     4621 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     4681 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     4741 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     4801 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     4861 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     4921 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     4981 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5041 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5101 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5161 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     5221 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     5281 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     5341 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     5401 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     5461 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     5521 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     5581 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     5641 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     5701 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     5761 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     5821 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     5881 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     5941 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6001 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6061 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6121 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6181 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     6241 gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc
     6301 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     6361 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     6421 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     6481 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     6541 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     6601 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     6661 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     6721 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     6781 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     6841 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     6901 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     6961 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7021 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7081 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7141 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7201 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     7261 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     7321 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     7381 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     7441 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     7501 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     7561 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     7621 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     7681 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     7741 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     7801 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     7861 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     7921 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     7981 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8041 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8101 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8161 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     8221 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     8281 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     8341 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     8401 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     8461 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     8521 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     8581 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     8641 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     8701 ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc
     8761 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt
     8821 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     8881 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     8941 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9001 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9061 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9121 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9181 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
     9241 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
     9301 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
     9361 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
     9421 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
     9481 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
     9541 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
     9601 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
     9661 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
     9721 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
     9781 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
     9841 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
     9901 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
     9961 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10021 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10081 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10141 gaagccaaaa tcaaggcctg gttggcataa tccctactcc actcctgcta tgcttaagaa
    10201 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    10261 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    10321 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    10381 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    10441 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    10501 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    10561 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    10621 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    10681 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    10741 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    10801 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    10861 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    10921 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    10981 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11041 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11101 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11161 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    11221 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    11281 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    11341 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    11401 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    11461 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    11521 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    11581 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    11641 gccagcgatc ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    11701 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    11761 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    11821 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    11881 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    11941 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12001 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12061 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12121 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12181 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    12241 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    12301 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    12361 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    12421 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    12481 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    12541 agcaggatcg atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc
    12601 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    12661 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    12721 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    12781 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    12841 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    12901 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    12961 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13021 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13081 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13141 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13201 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    13261 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    13321 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    13381 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    13441 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    13501 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    13561 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    13621 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    13681 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    13741 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    13801 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    13861 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    13921 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    13981 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14041 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14101 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14161 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    14221 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    14281 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    14341 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    14401 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    14461 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    14521 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    14581 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    14641 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    14701 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    14761 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    14821 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    14881 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    14941 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15001 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15061 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15121 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15181 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    15241 tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc
    15301 ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac
    15361 ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa
    15421 ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag
    15481 gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct
    15541 gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca
    15601 cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac
    15661 tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa
    15721 aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac
    15781 gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt
    15841 gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca
    15901 ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga
    15961 tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat
    16021 ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca
    16081 acatcttccc cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg
    16141 caacaagggc gaaagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg
    16201 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    16261 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    16321 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact
    16381 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    16441 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    16501 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    16561 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    16621 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    16681 caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    16741 cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    16801 tatcgacact ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    16861 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    16921 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    16981 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    17041 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    17101 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    17161 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta
    17221 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    17281 gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    17341 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg ccattttacg
    17401 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    17461 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    17521 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact
    17581 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    17641 gttagaataa acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc
    17701 tttttcgcga tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag
    17761 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    17821 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    17881 gattaaggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    17941 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca
    18001 gtgccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    18061 aagagataca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    18121 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    18181 aagttttcac gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc
    18241 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    18301 agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca
    18361 aagcggaaac ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg
    18421 gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    18481 ttctgattgg tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg
    18541 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    18601 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    18661 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    18721 ccgcgcacgc ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc
    18781 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    18841 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    18901 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    18961 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    19021 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    19081 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    19141 gatcagtacg ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgatacctgg
    19201 catactcagt gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc
    19261 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    19321 agaaaccagc agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag
    19381 ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    19441 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    19501 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    19561 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    19621 cggtttgccg atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag
    19681 cgagatgtta acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    19741 gtcaccagtc agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt
    19801 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt
    19861 taccagacgg aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc
    19921 ctgaataccg ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    19981 cgccacgcca agttggttta cacggttacg caggatatta atgttctgct gcaccgcata
    20041 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    20101 gctgctaatc accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    20161 tttagcatcg cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata
    20221 cgggatgccc ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt
    20281 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    20341 gccgagcttc atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag
    20401 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa
    20461 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    20521 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    20581 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    20641 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    20701 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    20761 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    20821 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    20881 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    20941 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    21001 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    21061 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    21121 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag
    21181 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    21241 gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc
    21301 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat
    21361 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    21421 gcattacgca gtcaaacata tcgataccgc gacgtacgcc ttcaagcagg tcttccggtt
    21481 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca
    21541 gaatacggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    21601 caaagccgat atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc
    21661 cctgaattat accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac
    21721 gcttcgccca acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat
    21781 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    21841 tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc
    21901 cctgttcggt gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg
    21961 taagaatcgg tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    22021 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    22081 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    22141 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    22201 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta
    22261 aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga
    22321 taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg
    22381 cattcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg
    22441 gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa
    22501 agatttgggt atcgtcaaag aacggttcga taagatcgct ttttgccgcc tgtgccgcgc
    22561 tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg
    22621 ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt
    22681 gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca
    22741 caaacgccat ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca
    22801 gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt
    22861 cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc
    22921 tgttgaggat atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt
    22981 ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag
    23041 gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct
    23101 caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca
    23161 cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga
    23221 aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc
    23281 gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa
    23341 cgcgcatgac actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct
    23401 ccctgcaacc tttacccccc tttggataga atataatcga tgaatttttt agctcacctg
    23461 catttagccc atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc
    23521 ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt
    23581 attgactgac aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg
    23641 ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc
    23701 gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat
    23761 gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga
    23821 gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc
    23881 aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta
    23941 tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca
    24001 caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga
    24061 atcccgtcag tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac
    24121 ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt
    24181 tgctgattca gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag
    24241 ccgccacgac gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg
    24301 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    24361 tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    24421 aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    24481 cgcgcagtat cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc
    24541 cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca
    24601 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc
    24661 atggcagcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg
    24721 acaatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc
    24781 atattattgc gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg
    24841 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
    24901 tcattcacca gactttccga ctgataatcc agcttcgtca agctggcata gccaagccag
    24961 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagagttg
    25021 tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc aggcatggga atcgccactg
    25081 tggttaaaca cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa
    25141 cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg
    25201 tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgctgt cacgccaagc
    25261 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
    25321 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct
    25381 gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga
    25441 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atcaggtaca
    25501 tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac
    25561 caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg
    25621 gagagatcaa tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta
    25681 tgcattggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
    25741 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
    25801 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
    25861 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
    25921 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
    25981 aacgcaagta ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc
    26041 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
    26101 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
    26161 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
    26221 aggcgatttg cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg
    26281 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
    26341 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
    26401 aaattttcgg tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
    26461 cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga atgctggcgg
    26521 caaaggtaat gcccatatgc tggaacgtca gtacgaacgg gctaccggca gtgccaacct
    26581 gattccacgg gtagatagac ataatgacga acagcgtacc aacgtagaac accagaatac
    26641 gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg
    26701 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta
    26761 ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
    26821 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
    26881 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca
    26941 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
    27001 gcaccgtcgg gaacaagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
    27061 caataaggat ttcaaaacag taggtccatc cggtaatgta acctgccagc gggccaaggt
    27121 tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca
    27181 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
    27241 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
    27301 ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagctta ttcttacttt
    27361 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
    27421 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
    27481 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
    27541 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
    27601 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
    27661 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
    27721 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagacacca
    27781 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
    27841 caaagaattc tgcacaagca caggtcaggc caaccgccgt gaccagacag gcgatgaaga
    27901 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
    27961 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacaga
    28021 acagcgccag gtacagcaaa gtcagaccaa cacccgccat caggccagcc cagacggtat
    28081 aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga
    28141 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag
    28201 aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
    28261 tcgcgacaac agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca
    28321 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
    28381 ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa
    28441 aggaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg
    28501 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
    28561 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
    28621 cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc gaccattgga gggaaaataa
    28681 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg
    28741 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
    28801 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
    28861 tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
    28921 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
    28981 accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa
    29041 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
    29101 tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag
    29161 gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt
    29221 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
    29281 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
    29341 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
    29401 acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc
    29461 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg
    29521 ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc
    29581 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
    29641 agcaattgcg tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct
    29701 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
    29761 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac
    29821 tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg
    29881 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
    29941 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat
    30001 tgcgtaaact ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag
    30061 cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
    30121 gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
    30181 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
    30241 ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat
    30301 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa
    30361 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
    30421 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
    30481 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
    30541 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
    30601 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
    30661 acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc
    30721 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga
    30781 gatgtcgggt tgagacttaa tccctgcatc ttaatcacct cttccaccgc cattggcgaa
    30841 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa tggcttggtg
    30901 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
    30961 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
    31021 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca
    31081 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
    31141 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
    31201 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
    31261 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt
    31321 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg accatgcttt
    31381 ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact
    31441 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag
    31501 cacaaaccca tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc
    31561 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
    31621 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
    31681 atatcatggc gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
    31741 tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc
    31801 gtgacattat ttccagccag gcggggctta acggtattga aaaacagcag catttactgg
    31861 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
    31921 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggttccagt aaaagtgacg
    31981 ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
    32041 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
    32101 ttcgctattg cggttccccc attccactga gttttgatga atgcggtaag agtaaatatg
    32161 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
    32221 taactcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac
    32281 agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg
    32341 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag
    32401 tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
    32461 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagacc
    32521 tggatgaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cgtaccctcg
    32581 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
    32641 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat
    32701 taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
    32761 tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
    32821 taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt
    32881 ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga
    32941 gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact
    33001 gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
    33061 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga
    33121 gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
    33181 ggcccgcact gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc
    33241 ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
    33301 aattaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt
    33361 gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag gcgttagccg aagaagaaaa
    33421 agcgcaacct caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca
    33481 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga
    33541 agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc
    33601 gaagcagtca gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca
    33661 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    33721 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    33781 acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct
    33841 ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    33901 tgttccccaa caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga
    33961 acagacgcaa cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca
    34021 gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    34081 ccagcgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccaccc
    34141 ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct
    34201 ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc
    34261 attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    34321 agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca
    34381 ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact
    34441 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    34501 tcaacagcaa attgaacaac gtcagcagca gctcttaacg gcattggcgg gttatgcact
    34561 gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    34621 gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    34681 accgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc
    34741 gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac
    34801 gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccaag cgcagtttga
    34861 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga
    34921 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    34981 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc
    35041 tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    35101 ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga
    35161 tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca
    35221 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    35281 caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    35341 ccggctgcac gtgcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt
    35401 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    35461 tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg
    35521 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    35581 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    35641 cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct
    35701 gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    35761 tacagagggg tgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    35821 ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt
    35881 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgagcgtg
    35941 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    36001 tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac
    36061 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    36121 acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat
    36181 ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    36241 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    36301 ctgggaacgt atttaagtca ggaatgctgg cgttacacct ttttattgat cgctgttttt
    36361 aatattgcgg tgatggcatc ggtctatttt tgggtgccag atattcgcga cgaggcgaaa
    36421 ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc
    36481 gccacgatgt ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg
    36541 atgtttattt ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agttgggcta
    36601 gggatggtgc tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc
    36661 attgcagcag tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc
    36721 ggcatgaaaa caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt
    36781 tcagcacctc tgcaaatatt gttactgcaa aatgccaaag gaggagagtt attaggtgcc
    36841 gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg
    36901 atgctgacgc tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt
    36961 gctgcgatgt catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt
    37021 ccggtgctgg cgaaaccact ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg
    37081 atgcggcgtg aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa
    37141 gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg
    37201 gccataacca cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg
    37261 gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa ctgcccaacc gtttgatata
    37321 aacggtctac attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca
    37381 cgacatgtgc cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta
    37441 ccggatcgct ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccgctca
    37501 aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt
    37561 taccgcaata acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta
    37621 gcggattgtg tccccactca cctgccgtgc cattgccgcc gatatgcgtc cgcccattga
    37681 atgccacgcc cgcgccgcat cccgtgccga taatcacggc aaataccgtc tgcgctcccg
    37741 ctgccgcgcc atctactgct tctgaaaccg ccagacagtt ggcatcattt gccagccgca
    37801 cttcccgctg caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg
    37861 aattgacatt cttcaccaca ccggtgtagg gcgaaatcga acctggaatg cccataccta
    37921 ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa
    37981 tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg
    38041 catcgcccag tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcactg
    38101 tactctcctt atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc
    38161 aaggccgccg acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta
    38221 tcatgctgtg gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg
    38281 cagaagagat ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca
    38341 tggcgaagat gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg
    38401 ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga
    38461 ttaaacaggc gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga
    38521 agaaaaccga aaaagattcg ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt
    38581 tcagccgttt tagccagaca atgatgtggc tcgacacggt taacggtttg attatggtgg
    38641 actgcgccag tgccaaaaaa gcggaagata cgctggcatt actgcgtaaa agcctggggt
    38701 cgttaccggt tgtaccattg agcatggaaa acccgattga actgacgctg accgaatggg
    38761 ttcgctccgg tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt
    38821 tgctggaaga tggcggcgtg atccgcgcga agaaacaaga tctgaccagc gaagagatca
    38881 ccaatcacat tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca
    38941 ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct taagttctgc gacgagctgc
    39001 gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca
    39061 tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg
    39121 aagcacaacg ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta
    39181 tccggcgtac gaagccacac caggcatata attattcgct acggcgagca ataattttta
    39241 gcgcagcaat attatgcgtt ttacgccgta actgatctaa tcgcctcatc cctattcgcc
    39301 tgtcagcagc acgcagaata ttcactaacc cccatttatc gctggtaaat tgttttgtta
    39361 acagctccgc ttttggggta tcaatatatt cacgcaataa atcagaaatt gaacaaatat
    39421 ccgtcaggta aggataccag caagtttcac cgtgaaaatt acgcactcca gcgttacctg
    39481 caataaagtc tttcgggtaa tcccagatca cgtttttatc caacgtgatc caataacgcg
    39541 gtaaatcggt acttccacca ttttggctgc gcatgggata acgggtacag tgaatttgca
    39601 ggttgatcgt tggcgtcagt aagtcataga tctcccgctg taacttgctc catggacgtt
    39661 gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc
    39721 ggttttcact aaatcacgta actgcgaaag atcgggttgt tcgacaaata agagtaatga
    39781 tggatcctct tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc
    39841 cagcgaccga tcctgaggct cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt
    39901 gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc
    39961 atcatctgat ttatctttta tcgtagccag ttccgcaaaa aatacctgga gatcttcact
    40021 gatatttata tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa
    40081 aggaaatatt attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg
    40141 ggaaggcgag gaattacaga tagcggcaca gataagaggt aggttcggca acttgaagat
    40201 gaaactcact gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg
    40261 tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag
    40321 cggtgctgaa ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc
    40381 tgctggaaaa gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa
    40441 actggccctg tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt
    40501 cacgcaaaat tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa
    40561 gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca
    40621 atccagcatg atgacatcag ccccttttgc cagtaattct ttcccagcat caatgatttt
    40681 ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg
    40741 ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg
    40801 atcttcaaca atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt
    40861 attacgcgca gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc
    40921 ctgattatcg agcacttcaa ccacaccttg cagatctcgc tccactttac gacgcgaaac
    40981 atgggccagc tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc
    41041 gtactccgcc atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt
    41101 atcttcgtca atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat
    41161 ggtcaggatc gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa
    41221 ttaccgggca agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag
    41281 cgtagcagca gacgtgcacg gcataatgta aagattccaa atgattccag taatggaatg
    41341 ttattgttta atattctaat tattagaaaa acataaatta tgaaaaatgt gacgcagatc
    41401 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg
    41461 tcctcatagc gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac
    41521 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt
    41581 cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
    41641 cttccttatt tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt
    41701 ctgtgccagg gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
    41761 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
    41821 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
    41881 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
    41941 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgc
    42001 cgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
    42061 ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac
    42121 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
    42181 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
    42241 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
    42301 aaagaaaaaa ttccacttaa agtaaaaatc tcaatacatc ccttactata ccaataaata
    42361 ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc
    42421 caccacttct ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt
    42481 ccgccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc
    42541 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc
    42601 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
    42661 tgccggacaa tttcgttatc ggcaacatcg aaacctgccg cgacgcgcag cgtacgaata
    42721 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
    42781 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
    42841 caaattgtca taaagtcatc ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt
    42901 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
    42961 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
    43021 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc
    43081 ctccccggat aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
    43141 gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat
    43201 taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
    43261 tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
    43321 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
    43381 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
    43441 ggaagtaatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
    43501 ctacgtattt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
    43561 cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga
    43621 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
    43681 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
    43741 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
    43801 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc
    43861 gatttcaacc actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
    43921 atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc
    43981 cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca
    44041 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
    44101 agcgccacaa tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa
    44161 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
    44221 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcga
    44281 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
    44341 acccagccaa acatcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc
    44401 ggagcactat tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc
    44461 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
    44521 cacagaatca tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac
    44581 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
    44641 gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc
    44701 agccaccacc agcccggcgg cgggtgcgaa accagcgttg aagcaatcgg taagaacatg
    44761 ccagccaggc caaccgcacg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc
    44821 tgatgttcgt tttgaggagt taaaggaggt tcctccccat gcgccgccgc ttttttgaaa
    44881 aagttttcat cattcattat ttttgggaac attcagaaac aattttccca aattatagag
    44941 acggggctaa aggcagaagt atgaaatttc gggtgagcca ctttcgtagc tcacccgtta
    45001 gcacta
//
LOCUS       DAZOYF010000014        44288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZOYF010000014 DAZOYF010000000
VERSION     DAZOYF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.014"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            17..1783
                     /gene="adeD"
                     /locus_tag="ACHUOF_000768"
     CDS             17..1783
                     /gene="adeD"
                     /locus_tag="ACHUOF_000768"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR0610065.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1829..3220)
                     /gene="uhpT"
                     /locus_tag="ACHUOF_000769"
     CDS             complement(1829..3220)
                     /gene="uhpT"
                     /locus_tag="ACHUOF_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR0610066.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3358..4677)
                     /gene="uhpC"
                     /locus_tag="ACHUOF_000770"
     CDS             complement(3358..4677)
                     /gene="uhpC"
                     /locus_tag="ACHUOF_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR0610067.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4687..6189)
                     /gene="uhpB"
                     /locus_tag="ACHUOF_000771"
     CDS             complement(4687..6189)
                     /gene="uhpB"
                     /locus_tag="ACHUOF_000771"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR0610068.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6189..6779)
                     /gene="uhpA"
                     /locus_tag="ACHUOF_000772"
     CDS             complement(6189..6779)
                     /gene="uhpA"
                     /locus_tag="ACHUOF_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR0610069.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6854..7144)
                     /gene="ilvN"
                     /locus_tag="ACHUOF_000773"
     CDS             complement(6854..7144)
                     /gene="ilvN"
                     /locus_tag="ACHUOF_000773"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0610070.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7148..8836)
                     /gene="ilvB"
                     /locus_tag="ACHUOF_000774"
     CDS             complement(7148..8836)
                     /gene="ilvB"
                     /locus_tag="ACHUOF_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR0610071.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8942..9040)
                     /gene="ivbL"
                     /locus_tag="ACHUOF_000775"
     CDS             complement(8942..9040)
                     /gene="ivbL"
                     /locus_tag="ACHUOF_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR0610072.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9605..9694
                     /gene="tisB"
                     /locus_tag="ACHUOF_000776"
     CDS             9605..9694
                     /gene="tisB"
                     /locus_tag="ACHUOF_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR0610073.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9818..9892)
                     /gene="ysdE"
                     /locus_tag="ACHUOF_000777"
     CDS             complement(9818..9892)
                     /gene="ysdE"
                     /locus_tag="ACHUOF_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR0610074.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9974..11158
                     /gene="emrD"
                     /locus_tag="ACHUOF_000778"
     CDS             9974..11158
                     /gene="emrD"
                     /locus_tag="ACHUOF_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR0610075.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11166..11663)
                     /locus_tag="ACHUOF_000779"
     CDS             complement(11166..11663)
                     /locus_tag="ACHUOF_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR0610076.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11660..12022)
                     /locus_tag="ACHUOF_000780"
     CDS             complement(11660..12022)
                     /locus_tag="ACHUOF_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR0610077.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12012..12359)
                     /locus_tag="ACHUOF_000781"
     CDS             complement(12012..12359)
                     /locus_tag="ACHUOF_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR0610078.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12468..12881
                     /gene="yidI"
                     /locus_tag="ACHUOF_000782"
     CDS             12468..12881
                     /gene="yidI"
                     /locus_tag="ACHUOF_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR0610079.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12928..14370)
                     /locus_tag="ACHUOF_000783"
                     /pseudo
     CDS             complement(12928..14370)
                     /locus_tag="ACHUOF_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(14405..16120)
                     /locus_tag="ACHUOF_000784"
     CDS             complement(14405..16120)
                     /locus_tag="ACHUOF_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFR0610080.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            16287..17180
                     /locus_tag="ACHUOF_000785"
     CDS             16287..17180
                     /locus_tag="ACHUOF_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR0610081.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17177..17991)
                     /locus_tag="ACHUOF_000786"
                     /pseudo
     CDS             complement(17177..17991)
                     /locus_tag="ACHUOF_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17991..19607)
                     /locus_tag="ACHUOF_000787"
     CDS             complement(17991..19607)
                     /locus_tag="ACHUOF_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR0610082.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            19902..20576
                     /locus_tag="ACHUOF_000788"
     CDS             19902..20576
                     /locus_tag="ACHUOF_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0610083.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(20615..22276)
                     /locus_tag="ACHUOF_000789"
     CDS             complement(20615..22276)
                     /locus_tag="ACHUOF_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR0610084.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22472..22900)
                     /gene="ibpB"
                     /locus_tag="ACHUOF_000790"
     CDS             complement(22472..22900)
                     /gene="ibpB"
                     /locus_tag="ACHUOF_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR0610085.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(23012..23425)
                     /gene="ibpA"
                     /locus_tag="ACHUOF_000791"
     CDS             complement(23012..23425)
                     /gene="ibpA"
                     /locus_tag="ACHUOF_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR0610086.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23419..23491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23730..24062
                     /locus_tag="ACHUOF_000792"
     CDS             23730..24062
                     /locus_tag="ACHUOF_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR0610087.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24064..25296)
                     /locus_tag="ACHUOF_000793"
     CDS             complement(24064..25296)
                     /locus_tag="ACHUOF_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR0610088.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            25397..26497
                     /gene="cbrA"
                     /locus_tag="ACHUOF_000794"
     CDS             25397..26497
                     /gene="cbrA"
                     /locus_tag="ACHUOF_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR0610089.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(26458..27795)
                     /locus_tag="ACHUOF_000795"
     CDS             complement(26458..27795)
                     /locus_tag="ACHUOF_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0610090.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(27870..29018)
                     /gene="dgoD"
                     /locus_tag="ACHUOF_000796"
                     /pseudo
     CDS             complement(27870..29018)
                     /gene="dgoD"
                     /locus_tag="ACHUOF_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(29015..29632)
                     /gene="dgoA"
                     /locus_tag="ACHUOF_000797"
     CDS             complement(29015..29632)
                     /gene="dgoA"
                     /locus_tag="ACHUOF_000797"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR0610091.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29616..30494)
                     /gene="dgoK"
                     /locus_tag="ACHUOF_000798"
     CDS             complement(29616..30494)
                     /gene="dgoK"
                     /locus_tag="ACHUOF_000798"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR0610092.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30491..31180)
                     /gene="dgoR"
                     /locus_tag="ACHUOF_000799"
     CDS             complement(30491..31180)
                     /gene="dgoR"
                     /locus_tag="ACHUOF_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR0610093.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31458..32114
                     /gene="yidX"
                     /locus_tag="ACHUOF_000800"
     CDS             31458..32114
                     /gene="yidX"
                     /locus_tag="ACHUOF_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFR0610094.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32160..32972)
                     /gene="yidA"
                     /locus_tag="ACHUOF_000801"
     CDS             complement(32160..32972)
                     /gene="yidA"
                     /locus_tag="ACHUOF_000801"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR0610095.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33086..33484)
                     /locus_tag="ACHUOF_000802"
     CDS             complement(33086..33484)
                     /locus_tag="ACHUOF_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR0610096.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(33724..36138)
                     /gene="gyrB"
                     /locus_tag="ACHUOF_000803"
     CDS             complement(33724..36138)
                     /gene="gyrB"
                     /locus_tag="ACHUOF_000803"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR0610097.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36167..37240)
                     /gene="recF"
                     /locus_tag="ACHUOF_000804"
     CDS             complement(36167..37240)
                     /gene="recF"
                     /locus_tag="ACHUOF_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR0610098.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37240..38340)
                     /gene="dnaN"
                     /locus_tag="ACHUOF_000805"
     CDS             complement(37240..38340)
                     /gene="dnaN"
                     /locus_tag="ACHUOF_000805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR0610099.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38345..39748)
                     /gene="dnaA"
                     /locus_tag="ACHUOF_000806"
     CDS             complement(38345..39748)
                     /gene="dnaA"
                     /locus_tag="ACHUOF_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR0610100.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40045..40125
                     /locus_tag="ACHUOF_000807"
     CDS             40045..40125
                     /locus_tag="ACHUOF_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610101.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40355..40495
                     /gene="rpmH"
                     /locus_tag="ACHUOF_000808"
     CDS             40355..40495
                     /gene="rpmH"
                     /locus_tag="ACHUOF_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR0610102.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40512..40871
                     /gene="rnpA"
                     /locus_tag="ACHUOF_000809"
     CDS             40512..40871
                     /gene="rnpA"
                     /locus_tag="ACHUOF_000809"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR0610103.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            40835..41092
                     /gene="yidD"
                     /locus_tag="ACHUOF_000810"
     CDS             40835..41092
                     /gene="yidD"
                     /locus_tag="ACHUOF_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR0610104.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41095..42741
                     /gene="yidC"
                     /locus_tag="ACHUOF_000811"
     CDS             41095..42741
                     /gene="yidC"
                     /locus_tag="ACHUOF_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR0610105.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            42847..44211
                     /gene="mnmE"
                     /locus_tag="ACHUOF_000812"
     CDS             42847..44211
                     /gene="mnmE"
                     /locus_tag="ACHUOF_000812"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR0610106.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        10725 a        12019 c        11342 g        10202 t
ORIGIN      
        1 tcaaaatgag aaaaatatga ataattccat taaccataaa tttcatcaca ttagccgggc
       61 tgaataccag gaattgttag ccgtttcccg tggcgacgct gttgccgatt atattattga
      121 taatgtctct attctcgacc tgatcaatgc cggagaaatt tccggcccaa ttgtgattaa
      181 aggacgttac attgccggtg ttggcgcaga atacgctgat actccggctt tgcagcggat
      241 tgatgctcgc ggcgcaacgg cggtgccagg gtttattgat gctcacctgc atattgaatc
      301 cagcatgatg acgccggtca cttttgaaac cgctaccctg ccgcgcggcc tgacgaccgt
      361 tatttgcgac cctcatgaaa tcgtcaacgt gatgggagaa gccggattcg cctggtttgc
      421 ccgctgtgcc gaacaggcaa ggcaaaacca gtacttacag gtcagctctt gcgtgcccgc
      481 tctggaaggc tgcgatgtta acggtgccag ttttaccctt gaacagatgc tcgcctggcg
      541 ggaccatccg caggttaccg gccttgcaga aatgatggac taccctggcg taattaacgg
      601 gcagaatgcg ctgctcgata aactcgatgc atttcgctac ctgacgctgg acggtcactg
      661 tccgggtttg ggtggcaaag aacttaacgc ctatattgct gcgggtattg aaaactgcca
      721 cgaaagttat cagctggaag aaggacgccg gaaattacaa ctcggcatgt cgttgatgat
      781 ccgcgaaggg tccgctgcca gcaatctcaa cgcactggca ccgttgatca acgaatttaa
      841 cagcccgcaa tgcatgctct gtaccgatga ccgtaacccg tgggagatcg cccatgaagg
      901 gcatatcgat gccttaattc gccgcctgat cgaacaacac aatgtgccgc tgcatgtggc
      961 atatcgcgtc gccagctggt cgacggcgcg ccactttggt ctgaatcacc tcggcttact
     1021 ggcacccggc aagcaggccg atatcgtcct gttgagcgat gcgcgtaagg tcacggtgca
     1081 gcaggtactg gtgaaaggcg aaccgattga tgcgcaaacc ttacaggcgg aagagtcggc
     1141 gagactggcg ctgtctgccc cgccatatgg caataccatt gcccgccagc cagtttccgc
     1201 cagcgacttt gccctgcaat tcacgcccgg aaaacgctat cgggtcattg acgtcatcca
     1261 taacgaattg attacgcact cccgctccag cgtctacagc gaaaatggtt ttgatcgcga
     1321 tgatgtgtgc tttattgccg tacttgagcg ttacgggcaa cggctggctc cggcctgtgg
     1381 tttgctcggc ggctttggcc tgaatgaagg cgcgctggct gcgacagtca gccatgacag
     1441 ccataatatt gtggtgatcg gtcgcagtgc cgaagagatg gcgctggcgg tcaatcaggt
     1501 gattcaggat ggcggcgggc tgtgcgtggt acgtaacggc caagtacaaa gtcatctgcc
     1561 gttacccatt gccgggctga tgagcaccga cacggcgcag tcgctggcgg aacaaattga
     1621 cgccttgaaa gccgccgccc gtgaatgcgg tccgttaccc gatgagccgt ttattcagat
     1681 ggcgtttctt tctctgccag tgatccccgc gctaaaacta accagtcagg ggctatttga
     1741 tggcgagaag tttgccttca ctacgctgga agtcacggaa taataaaaaa agcccggcgt
     1801 catgccgggc aaaagtcacc agttacgttt atgccactgt caactgctga atttttttct
     1861 cgcggcggat tttgcgttct tccattaccg ccactatcgc catcaggcag atacaaccaa
     1921 tcgcggcgat atccagcgcg gcgaaggtgc ctgcccagcc ggtaaggccg aataccggcg
     1981 tcccatcggc aatcattccc agacctaact tggcaaagct gtcaccaatc aggtaagcaa
     2041 aggtgccttt aataccatcg gcagcgccaa tcgctttttt aggtacaaag ccaacagcag
     2101 ccacaccaat caacaattgc gggccaaaga ccaggaaacc caacgcaaag agagaagcca
     2161 gatagatata ttcgttactg gcatgttgat acacaccgag cgtggcgata atcagcgcca
     2221 gcgcgatgca ggccaccagg ccacggcgac cgttcgccag gtcagagagc cagccccaca
     2281 gcagcgtacc gaccagcgca ccagcttcaa acagcgtaaa gccctgaatc gccaccgctt
     2341 tagagagttt cagttcctgg aacgcgtata cggttgacca ctggtcgata ccaatacgta
     2401 ccacatagag gaaaatgttg gcgaagcaca gcagccagat cactttgttt ttcagcacat
     2461 actcaacaaa gatctgccac ttggtcatat cggtagattc tgtctctttg tcctcttcgc
     2521 tgatctcctc gccgaacagt tcttcagctt tgccgaggcc ataagattcc ggggagtcgc
     2581 tgccgtaacg caggccgata aaaccgacaa tcagcgcgat aatcgacggg aagataaaca
     2641 tgccgatgac atggccatcg aacaggtaat ttgccccgaa cagcgccaca cctgctgcgc
     2701 ctgcaccgcc aaggttgtga gaaatattcc agaaaccgag gaatgtcccg cgtttacgac
     2761 gcggcgtcca tttggtgatg gtggagtaac tgcacgaacc gccggtactc tggaaaaagc
     2821 cgcttaaggc gtagaaggca atcatcagga acaggctaac cgagccgctg cccatactgg
     2881 cactgaagcc cagcatacaa atggcagaga ggataagcat gaacggcagg aattgtttgg
     2941 tgtttttgcc gtcggcgtag tatgaaacca acgttttacc cacgccataa gtgatggaga
     3001 aacccaggcc gatcatcccc agctgcgtca tgctcaaccc gtaggtcgaa atcatatcgt
     3061 tctgcgcgat gttaaagttc ttgcgaatca ggtacatcgt caggtagccg ataaagacca
     3121 ccaggtagga ttgcatgaac ggtttgaacc acattttgcg ccgcacttcg agcggaaggt
     3181 ccagggtcgg cttgcgaact tggtttagga aagccagcat gggttactcc tgaaatgaat
     3241 acctgcgtga ggcatgcatt gtaaaaatca gcagcaaatc aggcctgaga tagcgtccaa
     3301 gaaaaacctg gaaaatttct tagttttgcc ggatatgaag tgaaaaggtg agatgcatca
     3361 cgcttcacgc ggtgtctggg cgttcaaaaa gggcagtaac agcagtgcgg aaatcccggc
     3421 ggcgatagag ataaccacaa aaaatccgct ccagtgccag gtatcgagca ctttcgccag
     3481 cggccaacca gcaagcgacg cccccagata agcaaacaag ccgacaaacc ccgtcgccgc
     3541 ccctgccgcc tctttgtggg aacactctgc cgccgccata ccaattaaca tctgcgggcc
     3601 aaagacaaaa aaaccaatgg tgaagaagca ggttgcctgc atcacgtagc tggcaaatgg
     3661 catcagccac agcgagccga ctgaaagcaa aattccggcg gcgaaaatca aattcatcgg
     3721 cccacggttg ccgttaaaca atttgtccga gccccaaccg gctaccagcg caccgataaa
     3781 tccgcccagt tcaaacatcg tcactgccgt attcgccgtg accagatcga cgcccagcgt
     3841 ctcggacata tacaaattgc cccagtcgtt gatcgccgcc cggaccacat agaccagcac
     3901 atagcaaaac gaaagcagcc agatatacgg attcagcaac acatatttgg tgaggatctc
     3961 tttacgcgtc aaccctgccc cttcttgttg ttgagcaatt tccagcgcgt cgtgccgcca
     4021 ttcaccgacc gccggtaaac ctaacgcctg cgggcgatcg cgtagccgcc agcagagaaa
     4081 aatccccacg actatcgcca tacaaccagc aatcatcatc ccggcacgcc agccgtaatg
     4141 cagcgcgctc gctgccatca caatgggaat gagtgcgccg ccgacgttat gcgccgtgtt
     4201 ccataatgcc caccaaccgc cgcgctcggt acgtgaatac caggccgtta acagacgcgc
     4261 acacaccggt gaaccccagc cctggaaaaa ggcgttcagc acccagagca cggcaaacgc
     4321 ccatagcgac gtcgagaagc caaacagaat gttcatgatg cccgtggcga taagccctat
     4381 ccccataaaa taacgggcat ttgagcgatc gctgacaatg ccggagacaa acttcgacac
     4441 gccgtaagta atgtaaaaca gggtcgctaa caggccgata tcgctacggc tgagcacgcc
     4501 gttagcaagg atttctggta ccgcggcgtt aaaacttttc cgcgtgaagt aaaacagcgc
     4561 gtaacccagc cagatggtca gcagaatatg ccgacgccag tagcgataac gggcatcaat
     4621 ttcatgttta tcagtcatta atggcgcatc ggcaggcgct ttcagaaacg gcaacatcat
     4681 cgaaccttag acataacgtt gaggtagaga aacgctgaca cgcgtgccgt gcagacagga
     4741 aatgtgtaat gtgccgccca gcgccgttac gcgctcgcgc attccggtga ggccaaaacc
     4801 ttgttgcccg gaacccggcg gcaaaccgct gccatcgtct tcaatcacca gcatcaaccg
     4861 ttcatcctgc tgccagcctt gcagcgtaac cgcgctggca tcggcatgtt tcacaatgtt
     4921 gttcagccct tcctggcaga cacgaaacag cgtcacgcgc tggttttcgc ttaatgctga
     4981 ttcatcgata cgccattcga gatggctgac aatgccgcgc ccttccagct ccatttcccg
     5041 catcagtgag cggatggcct gctccagggt gagatcatcc agctggcgcg gacgtaaccg
     5101 tcctaacaaa cggcgcaccg cgtcgtaaac gcccagcgac agttgttcga tgagctgccc
     5161 gctctgcttc acactggcgt tatctgccgc cagccgctga acaatgcccg cctgagtacg
     5221 aatagcagtg atggtctgac cgatatcgtc gtgcagctca cgcgccacgt cgcggcgcac
     5281 gctctcttcg gtttctaaca aacgttcagc cagatgctga ttgcgcgcca gttccttttg
     5341 tagcgactgg ttaagttcac gcaaccgctg gatgcctgcg cccagtaaca accctgtcag
     5401 actttgcacc agcaacgaga gtaataaatc caccagatga tcgcgccagg tttgactggc
     5461 gatcagcgcg atggcgttca tcaacgtcgc aatcagcgct ccttgccagc catagtgcca
     5521 ggccagcgcg ataatcggca gcgccagaca gaatggcgta aagcgcgaca gttcgtccgg
     5581 caatcccaac tggagccaga gactgataac aaacagcagc aagtaccaga ccagatgccg
     5641 cccgcgccag ttgatgggct gagaaaccag tgacgggccg agcggcagcc aggtgttatt
     5701 ggcgagatag tgccagaaca ccagacatat cggggccagc gtcagaccgc cagttaaagt
     5761 cagcaacagc gcattccacg actctttgcc gtgccaaagc cagggcagcg actgcaacaa
     5821 cgccgccgcc gttaacgccg caccctgtaa cagcaaggtg cgccagtcac gctgatggcg
     5881 atagcgcgag atcagcgcta cgggcagtaa cgtcagcaaa ctaccgatca tcaataacgg
     5941 aaaatgggtt aaaccgaccg cctgcgttag ccagtaaatc agcagccact ccgcacccag
     6001 caatacgggc cagtatccgc gcgggcattg cagcattagc cccagacgca gaccaaacgg
     6061 aaataacagc accgccatat cagggcgctc aaccagatgc aggctgatac tccataggca
     6121 aaaccatgcg gcagagaaga taaaaaagca ggcaataacg gtaattaagc gggagaacaa
     6181 cgtcttcatc accagccatc aaacatgcgg cgcgccagct ctacgtcgtt actgacgccc
     6241 agtttttcca tcagattggc gcgatggacg tgtaccgttt tcggtgacaa gcccagttcg
     6301 gcggcaatct ctttcaccgc cattccttgc gccagttttt ccgccacctg gcgttcacgt
     6361 ttggtcagcg ggtcctgacg accggatgcc agtttaatgg caatatccgg cgtcagataa
     6421 cagccgcccg tggcaaccgt atgcaccgca gcaatgagtt catccgggct acagcgtttg
     6481 gaaagaaagc cgcgtgcccc cgcgttaagc gcctgctcaa ccagcgcagg actgtcgtga
     6541 acggagagca taatcgtcgc catacctttc ggcagctggc ttagcagctc cagaccggag
     6601 atatcgggca tggagatatc gcaaatacac acctgcacac cgcgccccgg cagccccgcc
     6661 agcgcctcgc gccccgaacc aaactcggca actacctgca aatcaggttc cagccccagc
     6721 agctgcgcaa agccggagcg gacgatgagg tgatcgtcta taagggcaac ggtgatcatg
     6781 gtcttgtcct ggcgggtaaa aaaaaacgcg cttaccttaa cgataagcgc gatgttgttc
     6841 aagccttgag cggttactga aaaaacaccg cgatcttgtt aaacatcgtc ggatcggact
     6901 gattacgctg cactttcacg acatcttcca gcttagcgat ttggcttatc atctgctcca
     6961 gacgctggtc gtcattgacc agtagccaga tatggctttt gtcgctgtcc tgaatcggca
     7021 gacaaagaat gccttcaacg ttaaaagcgc ggcgggcaaa aaggccacaa acgtgggtca
     7081 ttacgcccgg atggttgcga acggtgagct ccagaattac gttgttatga gttgtgtttt
     7141 gcatggctta ttcccccacc atttcagtat tcgccgcacc tggcggcacc atcgggtaaa
     7201 ctttttcttc ggcatcaatg cgcacatgga tcagcgccgg gccagggcga ttgatgattt
     7261 cctgcaatgc agcctgcgga tcggcttcgt tattcaaatc acaggtttcg aggccgaatc
     7321 cggcggcaat ctgcataaag ttgattttgc ccggataggt ggcggcaaaa acgccttgct
     7381 cgtagaacag actctgttgc tgatgcacca gccccagcgc ttcgttgttc atcagaatga
     7441 ttttgacatc cagctgattt tcactggcgg tcgccatctc ctgaatattc atcatcaggc
     7501 tgccgtcgcc ggagaaacac aacactttgc gatccgggtt cgccagcgcc gcgccaatcg
     7561 ccgcaggcag gccaaaaccc atcgtgccca gcccaccgga ggtcagccac tggcgtgggc
     7621 gattgagcgg ataagcttgc gcggtccaca tctgatgctg acccacatcg gtggtgataa
     7681 tcgcattgtc atcgacacag gcggcaacgg cgttgatcag gccgtaatgg cttaatggat
     7741 cgcacgcttt cgggattgga cacgggaatt cgcgctgcaa atccgctacc aactggtgcc
     7801 actctgcacg cggttgcgct tccacccgcg ggatcaattg cgccagcacg tcatcaacat
     7861 ccgcctgaat cgccacatgc ggctgcttga ttttacccag ctctgcgcga tcgatatcga
     7921 catgaataat tttggcattc ggacagaact gctcggtttt gccaatcgcc cggtcatcaa
     7981 aacgcgcacc gagcacaatc aacaaatccg cctcctgcaa aatatagttg gtactgcgca
     8041 cgccgtgcat ccccagcata cccagcgaca acggatgcgc ttttggcagc atgcccagcg
     8101 ccattaaagt catggtggta ggcagttgcg ctttctccgc cagttcacgc acccgcgcag
     8161 gcgcattgat cacaccacca cccagataaa gcaccgggcg tttggcagcg ttaatcattg
     8221 tagctgcgtc acgaatgctt tcttcgctaa aggcgggtgc aacggctttt tctgccaccg
     8281 cgggctgagc ttcaatctca aaaaccgccg tttgtacatc cttaggaatg tctatccaca
     8341 ccgggctagg gcggcctgat tgcgcaatgc ggaaggcatc gctcatgacc tgtgggagct
     8401 cttcgatatg tctgaccaga tagttgtgtt tggtgatggg gatagagatg ccgtaggtgt
     8461 ccacttcttg gaaggcgtcg gtgccgatca tcgaggcggg aacctgacca gtgatgcaaa
     8521 tcagcgggat ggagtccagc cgcgcatcag caatggcggt caccaggtta gtcgcacccg
     8581 gcccgctaca ggccatacag accgccggtt taccgtcggt gcgcgccatt ccctgagcga
     8641 taaaccccgc gccctgttca tggcgagcca gaatatggcg gatttgcgtg ctttggctta
     8701 aggcgtcgta aacaggcagg atagaaccgc ccggaatgcc cgtcacaatc ttaatgccct
     8761 gctgttccag gaaatgaacg ataaattctg cgccggtaaa gcgcttacgc gtcgatgttg
     8821 tgcccgaact tgccatgctc cagtcctttt cttctgggcc gactttccgg gaggtgctta
     8881 aacgaaaaac cccgcccggt ttgcgccggc ggggttttgg aatcgtgtgt tgttccagtc
     8941 cctacggcgc attgccgacg accaccacca cacgcacgac gaccactgcg gcggatggcg
     9001 cagttggtag tagttttgcg ttgaacatgg aagtagtcat tggggacctt gttggttttg
     9061 tgtttaacaa tatttataca agcacagctt tacaggggag acaatggaaa atttttcagc
     9121 aagggaaaat tgaggggttg atcacgtttt gtactgaatt gcagataaca aaaaaccccg
     9181 ccggagcgag gtttcgtcag ccgcctgcgg ctggtaaccg caaagcacac tgtattatgt
     9241 caacactgaa agtatacgtg ttccgcgcag aacgcgcaat ttcggcacga attttgacgt
     9301 atttagtgca tagttgagta tcgttcacag tttgcgtttt gtccaaatat tactgtttat
     9361 ttatacagta agcttctata atatcactgt acgcaatgtg ttatgcgggg gccgcatcgt
     9421 tacccggcgc actaagtcct ggctgaaacg ggtggtgccg tcagcgcctt aaccccgcgt
     9481 gagcacactg tgttatgtca acaagcacaa cgtttctcct tgagataccg cgtgcacaac
     9541 agctggcaac aggcagcgga aaggtacgtc agctggcagt gctcctgaac cacaggagac
     9601 gcgtatgagc ctggtggata tcgccattct tatcctcaaa ctcattgttg cagcactgca
     9661 actgcttgat gctgttctga aatacctgaa gtaattcaga ttcaagtcgc accaaagggg
     9721 agcgggtaac cgctcccctt ttatatttag cgtgcgggtt ggagtcggat gcgatgctga
     9781 cgcatcttat ccgccctacc atccctcccg gcaacattta ttgccgcttt tgtttacata
     9841 ttctgccgct aaacaattcc ccattcctgg cgtatatctg gctaacattc atcaatgtga
     9901 tagattcctc tcccgcattt atgggaatgc gtagtgactt attctaatta tttttataaa
     9961 agcatccgtg ataatgaaaa ggcaaagaaa cgtcaatttg ttattgatgt tggtattact
    10021 cgtggccgtc ggtcagatgg cgcaaaccat ttatattcca gctattgccg atatggcgcg
    10081 cgatctcaac gtccgtgaag gggcggtgca gagcgtaatg ggcgcttatc tgctgactta
    10141 cggtgtctca cagctgtttt atggcccgat atccgaccgt gtcggtcgcc gaccagtgat
    10201 cctcgtcgga atgtccattt ttatgctggc aacgctggtc gcggtcacga cctccagttt
    10261 gacggtattg attgccgcca gcgcgatgca ggggatgggc accggcgttg gcggcgtaat
    10321 ggcgcgtact ttgccgcgtg atttatatga acggacacag ttgcgccatg ctaacagcct
    10381 gttaaacatg gggattctcg tcagtccgtt gctcgcaccg ctaatcggcg gtctgctgga
    10441 tacgatgtgg aactggcgcg cctgttatct ctttttgttg gttctttgtg ctggtgtgac
    10501 cttcagtatg gcccgctgga tgccggaaac gcgtccggtc gatgcaccgc gcacgcgcct
    10561 gcttaccagt tataaaacgc ttttcggtaa cagcggtttt aactgttatt tgctgatgct
    10621 gattggcggt ctggccggga ttgctgcctt tgaagcctgc tccggcgtgc tgatgggcgc
    10681 ggtgttaggg ctgagcagta tgacggtcag tattttgttt attctgccga ttccggcggc
    10741 attttttggg gcatggtttg ccgggcgtcc taataaacgc ttctcaacgt tgatgtggca
    10801 gtcggttatc tgctgcctgc tggctggctt gctgatgtgg atccccgact ggtttggcgt
    10861 gatgaatgtc tggacgctgc tcgttcccgc cgcgctgttc tttttcggtg ccgggatgct
    10921 gtttccgctg gcgaccagcg gcgcgatgga gccgttcccc ttcctggcgg gcacggctgg
    10981 tgcgctggtc ggcggtctgc aaaacattgg ttccggcgtg ctggcgtcgc tctctgcgat
    11041 gttgccgcaa accggtcagg gcagcctggg gttgttgatg accttaatgg gattgttgat
    11101 cgtgctgtgc tggctgccgc tggcgacgcg gatgtcgcat caggggcagc ccgtttaagc
    11161 gcacgtcacc gcagcatcgt catcagctcc atgggagaac gatgctgctt tatcagatca
    11221 cgcatcaccc gcatatgcgg tgcggagtaa gaataaaacg cctgatagcc cgcacaaagc
    11281 acgctgtctg gcgtctcctg ccgagcgtgc gggcacaggc gatgccagtc gcacgcctgg
    11341 cagtcacggc gcggttgcgg gttttcgctg ccacaccatt tttgtaaggt ggcgtcaaaa
    11401 agctcaatcg agatacgact gatatctgcc cgcacccaac actcaaacag ggcaattaaa
    11461 aatttccccc acagctctgc tgtcaccgaa gcggccgtca gatcgccccg gtcatcaaat
    11521 tcaagatagg gaatgagctg tacggagcgg atgccccgct gtttgaaatt gtgataaaaa
    11581 tcggcaggtt gcagagcatc cttgtactct acaaccagct tatgcctgtc ctcttcagca
    11641 tcaatgacct gacttcctgt catgcgactc tctcaataaa tacgataagt tgatgtatat
    11701 gcgttacagc aaacagtatt gcgagagata acaccgcgag ggagatcaaa aatttgtcac
    11761 ggactacgtg aaattgggaa aaatcgctat tcgtgacatc cattaaattg cgattacggg
    11821 tgtagtgcca gaggatcagc gccacgatgg cgaggatgcc aatcgaaatc cagaataaca
    11881 tgcccgcctg gtgccagttg tgtttgatag ccaacgccat caacgcgccg tagcccagca
    11941 tggtacgaaa ccacgccagt gatgtgcgct ccggctgcag ccctgggtcg gcaatgcgtc
    12001 tggctttacg gctatccggc atacaacacc agtcccataa cgatcaccgc cacaaccatc
    12061 aatattaagc tgatgattaa taagctgttg gtgtagggca ggtcctcttt cagacgcatc
    12121 gctttttcat tgcgtaacca gcgcaggtag ccatacattg ctaacccgcc ggaaaacaga
    12181 cacaacaaca acgccagcag ttcacgaatg acgggcgtgg cgaaatctgg cgcaagttga
    12241 tcaagcccaa cccccgccgc cagaaatcct agtgcggtac ggatccacgc cagaaaagta
    12301 cgctcattag ccagcgagaa gcggtaatcg ggcgcttctc caaggcggga aattttcatt
    12361 gttgctcctt tgcaatttca ctaacgctct atctttagcg gtttttttta agaatttcgt
    12421 ccgcccgttt cctgatatgc ttttgccatc cgctacaagg aggtcagatg ggtatcatag
    12481 cgcaaaataa aatcagttca ttgggcatgc tgttcggtgc catcgcttta atgatgggga
    12541 ttatccactt ttcattcggc cctttttccg caccaccgcc aacgcttgaa agcatcgtgg
    12601 cggacaaaac ggctgaaatt aaaagaggtc ttctcgccgg aataaaaggc gaaaaaatta
    12661 ccactgtgga gaaaaaagag gacatggata tcgataaaat cctcgatcaa agcggtatcg
    12721 cactggccat tgctgcgcta ctttgcgcat ttattggcgg tatgcgtaag gaaaatcgct
    12781 ggggcattcg cggcgcgctg gtttttggta tcggcatcgt gtgcagcatt ttgctgatat
    12841 tcctgatttt ttccttcctg accggcggtt cgctggttta agcagcctga ataatgcccg
    12901 ccgggtgatc atcagcgggc attatcgtca gaatttctgt tttttctcct ccaccttcac
    12961 tccttgcgtc ggtaggccgg tgtcatagtc gcgtaccact ggcgaatagc catcttgtgg
    13021 gcgtgggcga aacgccccca tccagcgcgg tcgtgcatct ttacgccacg gacgcagact
    13081 ccattggtaa ctgcggaacg gatcgcgaat tttgtccatg taatccaata aggcgtcatg
    13141 cattttgctg cgaacgtctg caaaacggat atcatcgatc aggttatgca tttcatttgg
    13201 gtcattacgg cgatcgtaaa gttcatcact ggtgaagagg ttgagcacca gtttaaagtc
    13261 atccgtcacc cagcaacgca ccggaataaa accgccaaag ctgtcatgct caatctcgta
    13321 gcggttaaat tccaccatca cgccgcgtgg ctctttcacg gcaaggatat tttcccccgg
    13381 cagaatctct ggtttttcaa tatctgccag cgccatcatt gtcggcagta aatcaatatg
    13441 actgactggc gtatcgacct gtcgccgctc cccttgcggc gaacggatga tcagcggaat
    13501 acgggtgatg tcgtcataca tcgccgctcc tttactgatc agcttatgtg cgcccatcat
    13561 ttcaccgtga tcggaggtat aaatgaccca cgtattttca cgttgctctg gcgttaaggc
    13621 attgatgaca cgtccgattt ggtcatcaac aaagtcattg caggcaaaat agagcggatg
    13681 gtgataaagc ccgtcatcac cgactggcga tggcatcgcc tgcgcccata agcggtgatg
    13741 ttccggtttg ttcgccaggt catcctgtga tttctcgcca agttcgtagt aaaaatcagc
    13801 gtatttctct aaatactcca ccggacaggt gaacgggtga tgcggctcat catacgaaac
    13861 caccatcagg aagggttcct cggcgcgcac gggctattgc agaaaatcta ccgctcgatt
    13921 gctgatgcgg tgcgcccagg tgaaggtttc gtcaatatgg ttcgcctgta aatcttcgac
    13981 gctgtttagg ccattgcgcc acaggctgat ctctttttcc gtcagttcgc taagatagtt
    14041 cgccccatca aaccagtaat cagcatccca ctccggcgga cactcgccag tgccgaaata
    14101 gtcatgaccg tcgagatgcc atttgccgat gtaacaggtg tgatagccgg catccttaaa
    14161 gtagcgcccc atagtggaga tgtttttgcc tggcgcgacg ttgttggtcc acgggccgga
    14221 ctggttagcg tagataccgg tgaatagtcc ggcccgtgca ggcgtacaaa ccggtgaaca
    14281 agtgtaggcg gaattaaagc gaataccttc cgccgccaga ctatcaatat tttgcgtatt
    14341 cagcggttta ccgctatagc aaccgaccat attggtggcc tgggtatcgg tcaattgggg
    14401 cgtttcatag gctatccttc gcgtcgggag taaaggttac ggctgggtcg tgacgatgcc
    14461 accagctgtc aaaaatcagg tagacaatca ctacggcagc gatgaaataa ctgatcatcg
    14521 ctaaccagcg cgtaccgtag ccgccgaatt cagccagccc gacatagacg ccaatcatcg
    14581 cgaacaggat accaattgac gcgatcttga cgtttttcca cggtttcatg tccaccgcaa
    14641 acgcatcttt gaaggtgaac ggcgtggcgc gcggtttgat aaaaccgatc accagcatca
    14701 cgaccacgtt gatgcagaac gtacaggcca gcacatagag gaaatggaag tcgaacttca
    14761 ccagatagtt gatggtgatg tagctgatta tgccaatccc catcgccact tttgccgcca
    14821 gcgccgggat gcgcggaaag aaaaagccca taatgatgat ggtaaccagc ggcacgttgt
    14881 aaatgccgtt gagctgtttc atccagctat acagcccctg cggcgcgttc gcgatccacg
    14941 gcgctaccaa aaccgaaacg atagcgataa agaaaccaaa tttgcgcccg acggtgacca
    15001 gctgctgcgg ctcggcattc tggttaatga tgcgacggta aatacccata ctgaataatg
    15061 tactggcgct attcagaaag ccgttgaagg tactgatcac cgcaccaaat aacaccgcgc
    15121 cgaagaaacc ctccagtggc actggcagaa cgttattgac cagcgtcggg taggccatgt
    15181 cggctttcgg tagatcctga tacagatgaa acgcaatcaa ccctggcagc accagtacca
    15241 gcgggtcgag cattttcagc accgccgtta acagcgcccc cttttgccct tccgccaggc
    15301 tttttgacgc cagcgtgcgt tgcacgatgc cctgatttgt acaccagtaa aaggtgttca
    15361 ccagaattaa accggtaaat gccgcgccaa tcggcaaggg atcggtcggg ccaccgactg
    15421 agtttaattt ctcggcgtga acggtggtaa tttgctcaat gccctgcata aagctgccct
    15481 tgcctatcgc gatcaggcca aataccggca ccatcaaccc gccgataacc agcccaatac
    15541 cgttgatgga gtccgccact gccattgcgc gcagtccgcc gatcaccgca tacaaaattc
    15601 ccgccagacc aagcaaaatt accagtagcc agatagccgc accgtgagaa atctgtagcg
    15661 attccccgac gtgaaacagg ctgttgagcg ccaacgcgcc ggagtagaga acaatcggca
    15721 gaaagcagac gccggtggca atgaggaagc agaagtcgat gataatacgc gtcgttttat
    15781 cataacgttc ctccagaaaa tcggggatgg tggcaatgcc gcgcttcaga tagcgcggta
    15841 aaaagattag cgcgaggaag atcagcgtca ccgctgaagt cacttcccag cccatcaccg
    15901 acatgccgct tttgtaggcc tgcccggaaa gaccaaccag ttgttccgtg gaaaggttgg
    15961 ttaacattaa cgaagcggca ataaccggcg cttttagtga acgtccggca agaaaatagc
    16021 cttgttgcga tccggtatct gttttgcgaa ccttccacca ggtgatcacc gccaccagca
    16081 gcgtaaaacc gacaaaactc aagatttgta acgaattcat ctcatagccc ttatttatgt
    16141 ttatgtcccg gacgcatgaa tatagggaga ggtaaaatgc gtcgggtcag gcagtcagat
    16201 tcttaaaata caaacgtcgt atccctgaac ggattgtttt ctgttaagtt caggttgtga
    16261 gcataattcg ccggagggat attaaaatga atggaaaatt gcaaagttca gatgtaaaaa
    16321 acgaaactcc gtacaatatt cccttattga ttaacgaaaa tgtgatctcc agcggaattt
    16381 ctctgatctc gctctggcat acctacgccg acgagcatta ccgggtgatc tggccgcggg
    16441 acaagaagaa accgcttatc gccaactcat gggttgcggt gtataccgta caaggatgtg
    16501 ggaaaattct tttaaagaat ggcgaacaaa taactctgca tagcaactgt attatatttt
    16561 taaagccaat ggatattcac tcttatcact gtgaaggttt agtctgggaa cagtactgga
    16621 tggaatttac ccccaccagt atgatggata tttccgttgg tcagcaaagc gttatttata
    16681 atggcgaaat ttataatcag gaactcaccg aagttgctga gttaataact tcaccagaag
    16741 caataaaaaa taatctggca gtcgcttttc tgacgaaaat tatttatcag tggatttgtc
    16801 ttatgtccgc agatggtaaa aaagatccac aacggcggca aattgaaaaa ttaattgcca
    16861 ctttacatgc cagtctgcaa caacgctgga gcgtagctga tatggctgcc acgatcccct
    16921 gtagcgaagc ctggttgcgt cgtctgtttt tacgctatac cggcaagacg ccgaaagaat
    16981 attacctcga tgcgcgtctg gatctggcgc tatcgctatt aaagcaacaa ggaaactcgg
    17041 ttggcgaagt cgctgatacg ctcaacttct tcgactcctt tcatttcagc aaagccttta
    17101 aacataaatt tggttatgcg ccgtcagccg tgctaaagaa tacggaccag cacccaacgg
    17161 atgccagtcc acacaattaa cgtaactctg gccagtacgc tttgttggcc tccagcaact
    17221 catccagaat agctttggcg accgaggcgc tcggtaccgt tttcgacaac gtaatcgcct
    17281 gccacaggtg ctgatatgaa cgctgttccc aggcgtccac gaccagtttt tccaccgcca
    17341 cttgctgact cattaaccct ttctgaaact gcgggatatc gccgaccacc agtggttcag
    17401 gccgtagtag cgcacgcgca tctgtttgcg atcttgcagc ccaacaattt gcgccatccg
    17461 gctttcaata ccgattggca tgtcacagat gttgaggatt ttcgcattcg ggcgcagacg
    17521 acgcgtggct tctgcgacaa ttgctgccgg gttggagtag ttgagcatct aggcgtttgg
    17581 tgaatatttt tccatataat ccaccagttc caggacgccg ccaatggaac gcatgccgta
    17641 cgctattccg ccgggtccgc aagtttcctg accaacaaca ccgtggcgca gtgggatttt
    17701 ttcatccagt tcgcgcatcg ggtacttgcc gacgcggatg tgcgccatga caaaatcaac
    17761 gtcgctgaac gccacttcag gatcggtggt gtaactaaac gcaatgtccg gcgctttttc
    17821 tttaaggatg actttacagg cttcggcaat cacttcctgc cgcgcgccat cgttatcata
    17881 aaatttcagt gcacgaagcg ggaaacggtc ctgattcgcc aggagcatca acacgatgcc
    17941 tggcgtaaag gtgcttccac cgcctgcgac aaccactgag aatttggtca ttatactgcc
    18001 tccgtaatgg caacattttc tgctgattga tgagaattaa ttaagctatc gagctgttca
    18061 cgcaattggg atacatgcag accaatgatc acctgaatgg catcgccact acggaagacg
    18121 ccgtgcgctc ccagcttttt aaagacttcg tcatccagcg tttgtgacat gtcatgcagt
    18181 gcaatacgta gacgcgtcgc gcaattgtta atgctggaga tattgccgac cccgcccagg
    18241 gcttgcagga taccggcagc ctgatccagc tctttttttg gctctgcagc ggtggtttgg
    18301 cctcgcgagg ctttgtattc ggcttttgag tagagtttca cttccgcatc ttcacgtccc
    18361 ggcgtgcaca tgttgaactg cagaatcagg gtgcggaaaa ccacgaagta cagcagggta
    18421 aagcacaacc caatggcgat ttgggtcagc atcatatccg cgtggttgct gaacatcggg
    18481 atccagtttt gcggtaaaac ctggtcaatc agacctccgc ccatgttgcc caccacacca
    18541 aagagataca ttacggtcga cattgaggcc gccagcacgg cgtgtaccgc aaacagcaac
    18601 ggtgaaatga acaggaaggt aaattccagc ggttcggtaa ttcccaccag catggcggtt
    18661 aaggttgcgg gaatcagcaa gcccgccact tttacccgat tttccggtgc ggcagtgaag
    18721 tacatcgcta aagaaatgcc cacggcacca aagatttttg agttaccgtg cagggcaaaa
    18781 ccgccttccg ggaacaacga tttcagtggc tcggcgctca gactgaactc ttgcagatgc
    18841 tgcgcccagt acatctgaat gccgccttca acagctgccg gaccaaagat aaacggtccg
    18901 tagatgaagt ggtgtaaacc ggttgggatc agaatacgtt cgaggaaggt gtaaacccac
    18961 accccaagcg cacccgccga acgcaggaac gcttgcagag attcaatccc catttgtact
    19021 tttggccagc cgagcaatgt cagccaggca caggggatca tcaccaggaa ggcgataatc
    19081 accacataag acgtcccctg gaaaatgccg agaaaaaccg gcagtttttt atcgaacaga
    19141 cggttatgca gcgccgtcac aatgccggaa atgataattg cgccgataat gctggtatcg
    19201 agggttttaa tcccggccat cattgtcaga ccgctacctg ccaccgcgtc ctgagtgaaa
    19261 tcgacgccga agtagcttcc ccaggtcatt cccatcgcat tgatgaaata gttccaggtc
    19321 aggaaactca ccatcaccgc cagacaagca cgtccctgcg cttgcttagc aaggccaatg
    19381 ggtaaaccga cagcaaaaat cagcggcata ttacggaata ccgtccaacc gccctcttca
    19441 ataatgtgta cgatttgcgc gaataaactg ttcggatcgg tcagtgattc cccgacaaac
    19501 atcgggtttt gcagcaagat ggcaagaccc accacaatcc cggcgaaggg aaacagcagc
    19561 actggcgtga acatcgcgcc gccaaagcgt tgaatttgac tgagcatttc cacatcctct
    19621 tttctcaatt ctgtaagtag gaagcctgat tggttatgcg gcagacctgg agcgaggata
    19681 ggaagatgcg atcggcagaa catgacgacc caatgggatt cgtgatcgct ttcatgcttt
    19741 tcgttgggcg gtagagatct acgttttcgc gaaaaagtag acatctgtga gcacaggtac
    19801 acagcatcga acacatcttt aaaaaaaaga tttttttcaa tcgattaagc agaacttgtg
    19861 ggcgcattac ccgtgcttgc aggcaaaaaa gagagctaga gatgatctac aaaagcattg
    19921 cggagcggtt aagaattcga cttaattccg cggatttcac gctaaacagc cttcttcccg
    19981 gtgaaaaaaa gctggcggaa gagtttgcgg tatcgcggat gactatccgt aaagccattg
    20041 acctgctggt agcgtggggg ctggtggtcc gccgccacgg cagcggcact tacctggtgc
    20101 gcaaagatgt gctgcatcaa accgccagcc tgaccggact ggtggaggtg ttaaaacggc
    20161 agggaaaaac ggtcaccagc caggtgctga tttttgaaat catgcctgcg cctccggcca
    20221 ttgccagcca gttacggatt caaatcaacg agcagatcta cttctcccgt cgcgttcgtt
    20281 ttgtggaagg gaaaccgcta atgctggaag acagctatat gccggtaaaa ctgttccgta
    20341 acctttcgct gcaacatctg gaagggtcga agtttgaata tattgaacaa gagtgcggga
    20401 ttttgattgg cggtaattat gaaagcctga tgccggtgct cgccgataga ctgctggcgc
    20461 ggcaaatgaa ggtagcggaa cacacgccac tgctgcggat cacctcgttg tcatatagcg
    20521 agagcgggga gtttttgaat tattcagtga tgttcagaaa tgccagcgaa tactaggtgg
    20581 agtaccattt acggcgactc cacccggaaa aaagttaacc gatactccag aagagcaccg
    20641 ccagtaattg gggggtgata atgcgcagga acatcactaa cggatagaca gtggcgtaag
    20701 agagcgccgc cgcaccactg gttggatgaa gattattagc aaacgccagc gccggaggat
    20761 cggtcatgga acctgccagc atcccgcaca tggtcaggta attcattttg gctaacatcc
    20821 gcgccagaat gccaacagta atcagcggaa cggcggtgat cagggcacca taaccaatcc
    20881 agcttagccc ttcgccattg accagggtat tcacaaaatc cccaccagat ttcagaccaa
    20941 cgaccgagag gaacagcacg atccccagct cccgcagcgc gaggttggca cttggcggca
    21001 taaaccagta cagcttgccg atactgccga tacgcccgag gatcaacgcc ataatcagcg
    21061 gaccgcccgc cagccccagt ttcaacgcgg ccgggaatcc tggcacaaag acgggaatag
    21121 aacctaacaa tacgccaagc ccgatgccaa taaacactgg cagcatctga acctgttgca
    21181 gtttttgctg cgcattcccc agcacattgg caacggcatc aattgcggac ggacgcccca
    21241 ccaggttgag gatatcgccg aactgcaggc tgatatcgcc actggcgacc agttcgaccc
    21301 cggcacggtt caggcgcgag ataacaacgt catagcgttc tttaaagtgc aggtcgcgaa
    21361 tacgttttcc gagcacgttt tcattggtga ccaccacacg ctcgacgcgc aaatcagtgc
    21421 ctttcgtaga cagcgaggta tcgacctcct gaccaatcac cagttgcgca ttatgtaaat
    21481 ccgctggctg ccccaccaga tgcagcaaat cgcccagttg gataatggta tctggcgaag
    21541 gtacttttag ggtttcttct cgtttcagac gcgagcagat aattttgtcg ccgttgagaa
    21601 tcggtacatc tttaatggct aaatcatgca ggttagggtt ctcaacgcga atattgatag
    21661 tcttgatcag cgcgccgcca ttggtgcgtg aagactcgtg ctgctgagct tctgtctcga
    21721 cattgacgcg gaaaataacc cgcaacatcc acatggtgaa caaaatcccg caaatgccga
    21781 atggatacgc catcgcgtaa ctcatcccca tctgatcgac catttccatt ggtgtaccca
    21841 ggtcgcgcaa gatctgctgc cctgccccca gcgctggcgt attggttacc gcaccggaga
    21901 aaatccccag cactaccggc agtggaatat caaacagttt atgcaggatg gcggtaacca
    21961 gaccaccgat gatgacgatc agaacagcaa acaggttgag gcgtaatccg gagacgcgca
    22021 atgaggcaaa gaagcccggc cctacctgaa tcccgatggt ataaacgaac aggatcaggc
    22081 caaattcctg aataacatgc agcatatcgc tacttaatgt catccccgcc tgagaaacaa
    22141 aatggccgac gatgatccca ccaaacagca cgccgccaat acctaatcct atgccgcgaa
    22201 atttgacgtt gccgataaac aaaccgacga ctgccaccaa agccagaata ctgaccgtta
    22261 atgctatatc actcatcgtc catttctctt gagataacat tttaattgct acggattatg
    22321 gctgaatcgg tgaagcagat ttgccttata gcgcacatta tcacgggaaa gaacgtgccg
    22381 aaatatctta aacagtcgaa atttaacgca ttgttgaaga agaaagacaa aaaaagcccc
    22441 accagaatgg cggggcaaag agaatagcta gttagctatt taacgcggga cgttcgctga
    22501 tagcgatacg ctgcgctgcg atgggttcag gctcattacg aattaaatca atatgcagta
    22561 aaccgtttac gaaggttgcg ccagagactt ccatattttc agccagcgta aaactcaggc
    22621 taaatggctg attcataagc ccttgatgca gccatttttt ctcttctttt ggctgctccg
    22681 gcgtgccttt tacgctcagg cgcgtgcctt ccagttgaat ctctaaatct tcctgacgga
    22741 aacctgccag cgcaagggta atgcggtagt ggttatcgtc gcttttctca atgttgtacg
    22801 gcgggaagct ctggctttca ccggcgtttt gcagtgcgtt ggccagtttg tcaaaaccga
    22861 tccattgacg catcagtggg gataaatcga agttacgcat agtcatttct ccttctaaga
    22921 agcgagtaag tacctgcaaa tccgaagatt cgcatatgct ccctgacggc gagcatggag
    22981 atgtcaggcc gcgccaggcg gccttaggga attagttgat ttcgatacgg cgcggttttt
    23041 tcgcttccgg aatcacgcgt tcgagatcga tatacagcaa accatttacc aggttagccc
    23101 cacgaacatg aatgttctca gctaactgga atttgcgttc aaagttgcgt tcagcgatgc
    23161 cctggtacag ataggtgcgc tctttttgtt cgtcggcgtg agcacctttc accaccagca
    23221 gattatcctg ggcggtaatt tccagttcgc tctcagcaaa accagccaca gcgatagcaa
    23281 tgcggtaatg gttttcgtct accagttcaa cgttatacgg agggtagccg ccattactct
    23341 ggctctggtt gttttctaag tggttaaaca aacggtcaaa tccaatagca gaacggtaaa
    23401 gcggggataa atcaaagtta cgcataatca atagctcctg aaatcagcga gaatgtaaga
    23461 ccttccacaa tggacaggtc aggtagccag aacacccatc aggcgcgtcc tcatcgacta
    23521 cgatgtaaaa tgggtctgga aatgaacttt tcaagcctta tcagcggact tttttttgca
    23581 gtttatggtc tattgcatag actgaggggg cagcacgaaa cgttaaagtg cgatagccgc
    23641 cacagtgcga cgtaatgatg atgttatttt cagcaaggga tcgctataac tcatcaggcc
    23701 aacacccacg atcaacagat gaatttatta tgataagaaa tgtgttgtta gcgttcatga
    23761 tatgcagcgg aatgacatta ctcggaggat gctccagcgt gatgtcccat accgggggga
    23821 aagaaggaac gtatcccggc acccgcgcca gcgccaccat gattggcgac gatgaaacca
    23881 actggggcac taagtcgctg gcgattctcg atatgccgtt taccgctgta atggacacgc
    23941 ttttactgcc gtgggatgtg ttccgcaaag acagctcggt gcgctcgcgc gttgaaaaaa
    24001 gcgaagccaa cgcgcaagcc accaatgcgg ttattccacc ggccagaatg cctgataact
    24061 gatttagcga tcagtttcgg tgatccacag ctggccacct tccatccacg ccagccattg
    24121 accatccggc gagaagacca cggcgtccgc agaaggtgga ttggcgtggt tttcggttaa
    24181 atactcaacc tcgccgcttt gcgcatgggc acaaacaatt cgattatcca gcacaaagcc
    24241 caaccattct cctgacggat gccagttaaa tgcagactga atatccgttt tgttatgggt
    24301 taacttgcgc ggttcgccgc cctgtggcga gataagccac agttgcacaa tgccgttatc
    24361 atcacgcatt aaaaacgcga tttgcgtacc ctgcggatta cagcgcaccc agtggcgcgg
    24421 gacgttgact aaccccggat aagcccgatg atgggtaaag gttaaacgtc gctgcacgac
    24481 gccacgcggt ggcgcgggta gggttgtttc cgttccactt aacggcgcat cacctgccga
    24541 tttccagcta gcttcatctt gcggtaactc aacgataaat agctccggca ctttctcgcc
    24601 ctttggcgaa agtgtgtcgc caataaacgc cagcgcgtga tttcctaccc atccttcttc
    24661 ataagcacga ttgatttcat cactgccagg ctgcggcgtg ggcgtggttt tactcaccag
    24721 cacacaccag tggctaccgc tgtattcacg cggatgttgc ttttgtacgt tgaccgggcc
    24781 aaacggtgca gcaaccccga cgtttcgcaa atccagcgcc ggatcgagtt gatgcattac
    24841 atggtcgtta taggtaaagc tccccctttc accgttcggg ctaaagacat gcacatggct
    24901 gccaccgcgc agcgctcctg gcgtatacgg cgcggtaata tccattgcat cgagattgct
    24961 caccttgccg ccttcagcaa tgactccgcg tcgatgatgg aagtcgtaat gccatgtttc
    25021 atcaggattt tcagggccgt gaataaagac atatttctct gacttcggat gaacggtcac
    25081 cacgccgacg tgtgcgccct gtgacgcgcg atagataacc tcgacctcgc ctgtatggat
    25141 attcacacgc tcaatggtct cgccggtaaa cgacgcccca gaaggacgca cgtcaaatac
    25201 cagccactgg ctgtcgggcg tccaggtatt ggtatttgta ttggtattgg tattggtgag
    25261 caggtgatta cggggagcaa aggttatctg tttcatggcg cggtcctgat acaaaacggt
    25321 tcccgccatc atacttcaca aggacttcac tttcagacaa tagtcttttg ccatcgtgat
    25381 gaatgtgagt gaaaagatgg aacatttcga cgtggcgatt attggcctcg gcccggcagg
    25441 gtcggcgttg gcacgaaagt tagccggcaa aatgcaggtg atcgcgctgg ataaaaagca
    25501 ccagtgcggt actgaaggtt tcagcaaacc ttgcggcggt ctgctggcac cggacgcgca
    25561 gcgatctttt attcgcgatg gactgacgct tcctgtcgat gtgatcgcca atccacagat
    25621 tttcagcgtc aaaactgtcg acgtcgccgc atcgctcacg cgtaactacc agcgaagcta
    25681 tatcaatatt aatcgccatg ctttcgactt gtggatgaaa tcgctgatcc ccgccagcgt
    25741 tgaggtttat cacgatagcc tgtgccgaaa aatctggcgt gaggatgata aatggcatgt
    25801 catttttcgt gcagacggct gggagcaaca tattaccgcc cgctatctgg tcggtgccga
    25861 tggcgcaaac tcgatggtgc ggcgacatct ctacccggat catcaaatcc gtaaatatgt
    25921 cgctatccag cagtggttcg cagagaaaca tccggtgccg ttctactcct gcatctttga
    25981 taatgcgata actgactgtt actcatggag tatcagcaaa gatggttatt ttatctttgg
    26041 cggtgcctat ccaatgaaag acggtcagac gcgtttcacg acgctgaaag agaaaatgag
    26101 cgcctttcag ttccagtttg gcaaagcggt gaaaagtgag aaatgcacgg tgttgtttcc
    26161 ctcacgctgg caggattttg tctgtggtaa ggacaacgcc tttctgattg gtgaagcggc
    26221 gggatttatc agcgccagct cgctggaggg gattagctat gcgctggata gcgcagagat
    26281 tctgcgtgcg gtgttcctga aacagccgga gaagagcaac gccgcttact ggcgcgccac
    26341 acacaaactg cgtttaaaac tcttcggcaa gatagtaaaa agccgatgcc tgaccgcacc
    26401 ggctttaaga aagtggatta tgcgcagtgg tgtggcgcat attccacagt tgaaagatta
    26461 tccaacgcgc ttcacatcac ccaccagcag gatgtaagag agcgcgccaa tcaacgcgac
    26521 ggcggagata taaaccagtg caggcgcgaa accgtaaccc tgcgccaggt accccaccac
    26581 cagcggaacg gtgatgccgc ccagaccacc ggcgaagtta aacacgccgc cggttaaacc
    26641 aatcaggcgc atcggtgcca gagaagagac cagcgaccag gtaatcgaag caaaaccgtt
    26701 gccgaagaat gccagcgcca tcaggcacat aatcatcatc ggatcgttgg tgtagttagc
    26761 gcccataatg caggtggaga tcagcaagcc gcagataatc ggcgttttac gcgcaaagcc
    26821 cagtgaaaag cccttacgta ccagcagatc cgcgacccag ccagagagca ggacgccgac
    26881 aaacgccgcg aggaatggca ccgtggtcat aaagcctgct ttcagcgccg tgattccttt
    26941 ttcctgggtt aaatagttcg ggaaccaggt taagaaaaac cacagtgcag aagccaccgc
    27001 aaattgccca agataaacgc cgatcagttt acgatggaac accagtttcc agtctttggc
    27061 tgttaacggc tgacgcgcct ctttcttcac cggcgcatca ccatccacca gaccgccgcc
    27121 atcacgaatg taatccagtt cagctttgct gatacctttg gtcaggcgcg gcggctgata
    27181 aaccttaaac caaatcagcg accagataat gccgatacca ccagtgacaa tgaacaccca
    27241 gtgccagctc aacatctcct gaatccagat cagcagcgga gtcaaaaacg ccagaccgac
    27301 aaactgacca gacgtataaa aaccaacggc ggaagcgcgt tcatgttccg ggaaccagct
    27361 ggtcaccatc cggttattgg tcgggaaagc gggcgcttcg aaaataccgg ttatcgcacg
    27421 cagaccaatt aatgacatta atcccgtggc aaagccttgg aataaagtcg ccactgacca
    27481 accaaatatc gcaataaaat aagtcacgcg agaacctacg cgatctaaaa accaaccgcc
    27541 ggggatctgg catagcgtat aaagccaggc gaaggccgaa aatacatagc ccatttccgc
    27601 tttcgtaatg ccgaactctt cctgaatatg ggcggaagcc acggccaggt tagcgcggtc
    27661 aacataacaa atgaccaccg taataaagat catcaccagc gtcagataac gccgacgccc
    27721 cggctttgct gcattaacgg gaatatccat agcgagcttt ctccagattt tgggcatagc
    27781 gaagccgctc accatgccct gtaatttaca gagggttata tttttgtatt gctgttttag
    27841 tgcccgatga ggggcttacg tggcaggaat taccactctg ctacgctgtt atcttcatga
    27901 cgccagagcg gattacgcca gtccggggca tttttactga actcaataac tttagcttcg
    27961 tcgatttcca cacctaagcc cggtttcgtt aacggtttaa agaagccgcc aaccatgctg
    28021 aagtcttctt tgtttttcac aaagtcgagt aactccgcgc ctttgttgta atgaatgccc
    28081 atactttgtt actgaagtac cgcgttatag gaaacgaagt cgatatgcag gcaagccgcc
    28141 agtgcaatcg gtccgaacgg acagtgcggc gcaagggtca catcataggc ttctgccatt
    28201 ccagcaattt tgtagcattc ggtaataccg cctgcatggg agagatccgg ttgcagaatc
    28261 gaaataccgc ctgcctccag cacgcgttta aaatcgaaac gtgagaacat gcgctcaccc
    28321 gccgccagtg gaatatgcgt ttgtgccgcc aatttcgggt agtattcggc ttgttccgcc
    28381 agcaccggct cttcaataaa cagcgggcga tacggctcca gttctttaat cagcactttc
    28441 gccattggcg cgctgacgcg accgtggaaa tcaagaccaa actcaatctg attgccaaaa
    28501 gcttcacgaa tttgtgccac ggtgttgact gccgcatcta ccgcgcggga gttatcaatt
    28561 agccccagtt cttcacaacc gttcagtttg aaggtatcga agccgatttc gcgcagggtt
    28621 ttaatgccgt cgataacatc cgccggacga tcgccgccga cccaactgta ggctttaatt
    28681 ttgtcgcgaa ccaggccgcc catcagttgc cagactggcg cattcagcac tttgcctttg
    28741 atatcccata acgcctggtc gatcccggcg atggcgctca tcaggattgg accgccacga
    28801 taaaatccgg cgcgatacat cacttgccat aagtcgttga tgcgcgaagg atcctgacca
    28861 atcaaatagt cacccagctc gtgaactgcc gcttccaccg tacgggcgcg gccttcaatc
    28921 acgggctcgc cccaaccgac cacgccttca tcggtttcaa ttttcaggaa catccagcgg
    28981 ggaggtaaac gatacgtggt aattttggtg attttcattg cactgcctct cgatacgcct
    29041 taacaaatgc tgctgcctgc tgcgcggtgc gttctacgga ctgcccggcg cgataaagat
    29101 cgctgcctaa gcccgcccca acacaacctg cgtctatcca ctgcgccagg ttttctggcg
    29161 tcacgccgcc aacggcaaag actgcgatgt ccggtggtaa taccgctttt aacgctttga
    29221 tgtattgcgg accaaaagcc gatgacggaa atattttcag cgcctgcgcg cccgcttcga
    29281 gcgcggtaaa ggcttcggtc gccgtcgcgc agccggggca aacggtcatg ccgtagccca
    29341 ccgcgcggcg gatcacttca ctatggatat tgggcgtaac gatgagctga cagcccatcc
    29401 tggcgagcgc atcgacctgt tcaggtttca gtaccgtacc tgcgccaatc aacgccttgt
    29461 cgccatacgc atcaacgatg gcgggaatgc tctgctccca ttgtggggaa ttcagcggga
    29521 tttcaaccgc gtcgaacccg gcgtcaatca ccgcgccaac atgcgccagc gcctcgtcgg
    29581 gcgtaatgcc gcgcaaaatg gcgatcagcg ggagtttagt ttgccactgc atgagcgatg
    29641 ctccttatac cagcctgaaa tgccgtgtcg cccgccaccg ccgccacgtc gcaacccatc
    29701 gcctgaaagg cttgctggta gcgcgcggtc agcgatgttc cggcgacaag ggtgatggcg
    29761 tgttgatggg tcacatagtc gcgcatactg gcaacctctg cgccaatcaa caaaccagag
    29821 agaaattcgc tgacctgttc gcggggaagt gttcccagca catgcgaggc gcgaacttca
    29881 aaaagctgcg gcaatatggc gggcgcatta aggccgcgct caaggccagc cgcgaaggca
    29941 tcggcagagt tttcctgcgg cggcaaacct gcgccaatca atgagtgatt taacagtaaa
    30001 tgatgtaatt caccggtcat cacggtgcga aaatcgttga tttgctggct atcggcctgc
    30061 acccatttgc aatgcgtacc gggcataaca taaagagagg aaggagccag agctcgcgcg
    30121 ccgatcaatt gtgtttcttc gccgcgcatc acattgtggt tatcgtcatg agagacgcat
    30181 aatccgggaa taatccagat attgtcgcca acagacgtta attgttcgcc aatagacgaa
    30241 aaacgggcag gaacagataa atacggagca actttccagc cgacattgct gccgaccatt
    30301 cctgccatca ccactggcgt attttcttca cgccagtcgg tcgtgacttc tgctaacact
    30361 gcagccggag attttccgtt caggcgcgtg acgcctgctt ctgattgcct gctctccagg
    30421 cagtggtcgc cctgataaag ccaggcgcgc agattggtcg atccccagtc aattgcgatg
    30481 tagcgagctg tcatgtgatt tcctttaacc ttcgtgtcga gctggcaatc atggtaagcg
    30541 ccgcctgctc tgccgcatcg ccgtcctgat gccgtatcgc atcgaacagc gccttatgtt
    30601 cctggagcgt ttgcggcatg ttggcctcat cgcccatcca ggttcgttca aaaaccgccc
    30661 gctgcagcga actgatcgca atgctaagtt gctgtaacac cgggttatgc accgactgca
    30721 gcaccgcctc gtggtagcga atatccgctt cgttaaacgc ttcgcggtcc tgattgttgg
    30781 caatcatctc gttcagcgcc gattcaatct gcgccagatc gctggaggtc gcgcgctctg
    30841 ctgcccaacg ggcaatcgcc ggttccacca gatttcgcac ttcgctcatg gcactgataa
    30901 gccgtgggtc gtagtcgttt tccagcaccc attgcagtac gtcagtgtcg aggtaattcc
    30961 actggttacg cggtgccaca aacgccccgc gataacgttt catttcaatc agccgcttcg
    31021 ccatcagcga acggaacacc tcacggatga tgttgcgcga ggttgcaaac tcttcacaga
    31081 gctccgcctc agccgcaagt ggcgagccag gtacgtattt gccgtgaacg atctgtttac
    31141 ccagcgtaat gacaatgcga tcggttttat tgagagtcat ggagagtcct tgtgctctgt
    31201 atgttcttct ctactttacc ccgatcgatg cataacgcgg caactttgta gtaccagcgt
    31261 gatgacgttc gcgtttgccg cgcgcgtaat gtagtacaaa cttatattgt tgcgctacaa
    31321 tttagatcaa aaaaagaaca acacataaaa aaatgacatg cgtcgggcag aaatctgaaa
    31381 agggatatca ggcgctaaac aggagggaaa gaagagtatg ctttcaacgg cttagctact
    31441 cgtttaaagg attaatcatg aagttgaatt ttaagggatt ttttaaggct gccggtttat
    31501 tcccactggc gctgatgctt tcaggctgta tctcgtatgc tctggtttcc cataccgcaa
    31561 agggtagttc aggaaagtat caatcgcagt cagacaccat cactgggcta tcgcaggcaa
    31621 aagatagtaa tggaacaaaa ggctatgttt ttgtagggga atcgctggat taccttatca
    31681 ctgatggtgc cgatgacatc gttaagatgc tcaatgatcc agcacttaac cggcacaata
    31741 ttcaggttgc cgatgacgca agatttgttt taaatgcggg gaaaaagaaa tttaccggca
    31801 caatatcgct ttactactac tggaataacg aagaagaaaa ggcactggca acgcattatg
    31861 gttttgcctg tggtgttcaa cactgtacca ggtcactgga aaacctaaaa ggcacaatcc
    31921 atgagaaaaa taaaaacatg gattactcaa aggtgatggc gttctaccat ccatttaaag
    31981 tgcgatttta tgaatactat tcacccagag gcattccgga tggtgtttcc gcagcattac
    32041 tgccagtgac tgttacactg gacatcatta ctgcaccgct gcaatttctg gttgtatatg
    32101 cagtaaacca ataatcagta agcgggcaaa cgcgtttatg ctgtttgccc gcccacagat
    32161 taattcagca catacttctc aatagcaaac gccacgccat cttcaaggtt ggattttgtg
    32221 acaaagttcg ccacttcttt caccgaagga atagcgttat ccatcgccac accgacgcct
    32281 gcatattcaa tcattgcgat gtcgttttcc tgatcgccaa tcgccatgat ttcttccggt
    32341 ttaataccta acacgtcggc cagtgatttc acacccgtac ctttgttaac gcgtttatcg
    32401 aggatttcga ggaagtacgg cgcacttttc agcacggtat atttctcttt cacttcctgc
    32461 ggaatacgcg cgatagcctg gtcgaggatg gcgggttcat caatcatcat cactttcagg
    32521 aactgggtat tggggtccat tttctccgct tcgcagaaca ccagcggaat tgtggcaacg
    32581 aaggattcat gcaccgtgta gtagctgata tcgcggttag cggtgtacag cgtggtgcgg
    32641 tccagggcgt ggaaatgaga accgacttcg cgagagagtt tttccaggaa acgatagtcg
    32701 tcatagctga gagcggtttg cgccacggtg ctaccatcag cggccttctg taccagcgcg
    32761 ccgttataag taatgcagta gtcgcccggc tgttccatat gcagctcttt caggtagttg
    32821 tgcacacctg catacggacg acccgtcgtt agcacgacat tcacgccacg ggcgcgagct
    32881 gcggcaatcg catttttaac ggcgggtgaa atggtgtgat cgggcagcag aagggtgcca
    32941 tccatatcga tagcaatgag tttgatagcc atgagttccc aaggtagatt agttcctgac
    33001 ccatgctaac gcgattccgc tcaaaaatca gtacaacacc cgagggaaaa ggggatgcaa
    33061 cgcgggttgc atgctcccca tttgcttagc ggaagagttt ccctttcagc agttccatgc
    33121 ctgcggaaag cagatcgtta ttggcttgtg ctgacacttc accttgcggt gagagcgcat
    33181 caataatctt cggcaattgc tctgccagta aactggaagc cgtactggta tccacgccaa
    33241 gtttttgccc gagatcggac accgcatttg tgccgagcgc cgactccagt tgctcgccgc
    33301 taactgattg atttctctgt tgattactca accaggttga gagaatggcc cctaagccgc
    33361 cactttgcag tttttccagc agcacctgaa tgccaccctg ctcctcaacc caacttaaaa
    33421 tcgcctgata tttccccgca tcgcctttca gaaaggcacc gacaacttca tcaaaaagcc
    33481 ccatgataat cacctgtaaa gcgttacgtg ttgacccaaa aagtatagat ctgcgggatg
    33541 ataattgcgg attgcagaaa taaaaagggc ggagatgatc tccgcccttt tcttatagct
    33601 tcttgccgga tgcggcgtga acgccttacc cggcctacaa aatcatgcaa aatcaatata
    33661 ttgcaggagt ctcgtaggac taataagcgt agcgcatcag gcacgctcgc atggttaacg
    33721 ccattaaata tcaatattcg ccgctttcag ggcgttctct tcaataaacg cacgacgcgg
    33781 ttctacggcg tcgcccatca gcgtggtgaa caactggtca gcggcaatcg catccttaac
    33841 ggtaacgcgc agcatacgac ggctttccgg gtccatggtg gtttcccaca gctgttccgg
    33901 gttcatctcg cccagacctt tataacgctg gatggagagg ccacgacggg actctttcac
    33961 cagccagtcc agcgcctgct cgaagctggc taccggctga cgacgctcgc cacgttcgat
    34021 aaacgcatct tcttccagca agccacgcag tttctcaccc agcgtgcaga tacgacgata
    34081 ttcgccaccg gtgataaact cgtgatccag cggatagtca gtatccacac cgtgggtacg
    34141 cacgcgaaca atcggctcga acaggttttg ttcggcattg gtgtggacat caaacttcca
    34201 ctggctgccg tgctgttctt tgtcgttcag ttcgctgacc agcgcgttca cccagcgggt
    34261 aacggtctgc tcatcagaaa ggtcagcttc cgtcaacgtc ggctgataga taagctcttt
    34321 cagcattgct ttcggataac gacgctccat gcggttgatc attttctgcg tcgcgttgta
    34381 ctcagatacc agtttctcta acgcttcgcc agccaatgcc ggtgcactgg cgttggtgtg
    34441 cagcgttgcg ccgtccagcg cgatagagat ctggtactga tccatcgctt cgtcgtcttt
    34501 aatgtactgt tcctgcttgc ctttcttcac tttgtacagc ggcggctgag cgatgtagac
    34561 gtgaccgcgt tcaacgattt ccggcatctg acgatagaag aaggtcaaca gcagcgtacg
    34621 aatgtgcgag ccgtcgacgt ccgcatcggt catgatgatg atgctgtgat aacgcagttt
    34681 gtccgggttg tactcgtcac gaccgatacc acagccaagc gcggtgataa gcgtcgccac
    34741 ttcctgagaa gagagcatct tatcgaagcg cgctttctcg acgttaagga ttttaccctt
    34801 cagcggcaga atcgcctggt tcttgcggtt acgcccctgc ttcgcagagc cgcccgcgga
    34861 gtccccttcc accaggtaca gttcggaaag cgccggatcg cgttcctggc agtctgccag
    34921 tttgcccggc aggccagcta agtcgagcgc acctttacgg cgggtcattt cacgcgcgcg
    34981 acgcgccgct tcacgggcac gggcagcatc gataattttg ccaaccacga ttttcgcatc
    35041 agttgggttt tccagcaggt attccgccag cagttcgttc atctgctgtt caaccgccga
    35101 tttcacctca gaagaaacca gtttgtcttt ggtctgggag gagaatttcg ggtccggcac
    35161 ttttacggaa acgaccgcaa tcaggccttc acgcgcatcg tcaccggtgg cgctgacttt
    35221 ggcttttttg ctgtagcctt ctttgtccat gtaggcattc agggtacggg tcatcgccgc
    35281 acggaagcct gccaggtgag taccgccgtc acgctgcgga atgttgttgg taaagcagta
    35341 gatgttttcc tggaagccat cgttccactg cagcgccact tcaacgccaa taccgtcttt
    35401 ttcggtggag aagtagaaga tatttgggtg gatcggcgtt ttgttcttgt tcagatattc
    35461 aacgaacgct ttgatgccgc cttcatagtg gaagtggtct tctttgccgt cacgcttgtc
    35521 gcgcagacga atggaaacgc cggagttgag gaacgacaac tcacgcagac gtttcgccag
    35581 aatttcatat tcgaactcgg tcacattggt gaaggtttcg aggctgggcc agaaacgcac
    35641 catggtgccg gttttttcag tctcgccggt aaccgccagc ggagcctgcg gtacaccgtg
    35701 ttcgtagatc tgacggtgaa ttttaccctc gcgctgaata accagctcca gtttttgcga
    35761 cagggcgttt actaccgaaa caccaacgcc gtgcagaccg ccggacactt tataggagtt
    35821 atcgtcaaat ttaccgcctg cgtgcagaac ggtcatgatc acttccgccg ccgatacgcc
    35881 ctcttccggg tgaataccgg tcggaatgcc gcgcccgtca tcctgtacag agacagagtt
    35941 atcggcgtga atggtgacga taatttcttt acagtgaccc gcgagcgctt cgtcgatagc
    36001 gttatctacc acctcgaata ccatgtggtg cagaccggtg ccgtcatccg tgtcgccgat
    36061 atacataccc gggcgcttac gcaccgcatc cagccctttc aggactttga tactggagga
    36121 gtcataagaa ttcgacatca acgtttctcg ctcatttata cttgggttaa tccgttattt
    36181 tacccttttc cacggtaaac atcttcgaat tttcgtccga catgtctata acgtgttcag
    36241 cactgatcgc gctgacaaag acctgtgatt gcgtcgcttt taagcggctg gcaagcaacc
    36301 cgcgacgctc atcatcaagc tcagaggcaa aatcatctat caggtagaga caccgccgcc
    36361 cgctttcacg agtgaggaac tctccttgcg ccagacgtaa ggcgcacatc aacagcttaa
    36421 gctgcccacg cgataaggta tcttccaccg gcgcaccgtc ggcgcgaatg cgtaagtccg
    36481 ctttatgcgg gccatgcgcg gtgtaggtta gctggcgatc gcgttcaaaa ttacgttcca
    36541 gcacctcagc gtactctgtc tctttctccc agccgcgctg gaaagagaaa gtcagagaaa
    36601 actcagggag aaattgctta caggtatcat ccatatcggc cgcgataccg gcgctatact
    36661 ccgcgcgcca ggtgctgatt tgctccgcca gcgggatcag ttctttatcc cacgggcgta
    36721 gctgttcgta acgagtcacc tggcgcagcg ccgcattgcg ctgcttgagc aatcgcttga
    36781 gattgctcca ggcggtgaaa aatccaggtt cgttgtgaaa gcatccccag tcgaggaatg
    36841 ctcttctgta tttggggccg ccgttgagta aagtaaaccc ttctggcgtt atcaactgca
    36901 tcggcatcag gtgcgccagt tccgcgactt tatgcccgtc cgtaccgtca atacggactt
    36961 tgctgtcgcc ctgtttgtct ttggttaagc caatcgccgt ctcgcgctct tcgccctgta
    37021 atcgcccgtg gagaacaaat gcctcctgct catggcgaat gacgcgacca atctgcaaac
    37081 tgcgaaacgc ccgaccatgg ccgagcgtat agatggcttc cagcacgctg gttttgccac
    37141 tgccgttggc acctaccaga aagttaaagc cgggagataa agcgagatcc gcggtttcaa
    37201 tattgcggaa atcgcggatc aacaagcggg tgagggacat tacagtctca ttggcatgac
    37261 aacataggca gccgactgtg atgcggcatc ttcaatctgc acgctggaaa ccgaatcggt
    37321 cagcatcatg cggacgtttt cgcatttcag cgcgttcaga acatccagca catagctgac
    37381 gttgaagccg atttccatct ccgcaccgct ataggtaacg tcgaggatct cttccgcttc
    37441 ttcctgttcc gggttgttgg cagtaatttt cagctggttt tcgctgacat acaggcgcac
    37501 accgcggaat ttctcgttag agagaatcgc cgcgcgagca aacgcctgct tgagcagatc
    37561 gcagcctgct tccagatgtt tgtccgggtt cttcggcaga acgcgacggt aatccgggaa
    37621 gcgaccatcc accagtttcg aggtgaaaat aaagtcgcca acgtgggcac gaatgttatt
    37681 gctgccaatc tgcacacgca gcagattgtc gccgccgtcg agcatacgca tcagttcaat
    37741 cacgccttta cgcggtacga tcaccgaatg gcttggcaaa gattgaccaa ttggcattga
    37801 acagaccgcc agacggtggc cgtcggttgc cacggtgcgc agttcttcac cttcggtttc
    37861 aaacagcata ccgtttaagt aatagcgaac atcctgatgc gccatcgaaa actgggttgc
    37921 ttcaatcaga cgcttcatcg tcgcctgcgg cagggtaaat tcgacttcgc tctgccagtc
    37981 atcgaggttc gggaaatccg ccgctggcag ggtagacagc gaaaaacggc tacgcccgga
    38041 gcgcaccagc atccgttcac cttccagctg cacggcaatt tccgcgcctt caggcagacc
    38101 acggcagata tcaaagaatt tgcgcgccgg aacggtcgtc gctcctggct cgtgtggctg
    38161 aaccagcgca acgcgtgcca ccatttccat ctcgagatca gtaccggtca gcgacaacgt
    38221 accgtcagca acctgtaaca gcagattacc gagaatcggt agcgtaggac gaccacctaa
    38281 cggaccgctc acctgttgta gcggttttaa taaatgctca cgttctacgg taaatttcat
    38341 aggtttacga tgacaatgtt ctgattaaat ttgaaaaatc ttctttgata tcgtggctct
    38401 cttcacgcaa ctgctcgatc ttacggcagg catgaagcac cgtcgtgtgg tcacgcccac
    38461 caaacgcatc gccaatctct ggcagactgt ggttagtcag ctctttcgct agcgccatcg
    38521 ccatctggcg cggacgcgcc accgagcggg atcgacgctt ggaaaggaga tccgcgactt
    38581 tgatcttgta gtactccgcc accgtcttct gaatattgtc gatggtgacc agtttttcct
    38641 gcaatgccag caagtcgcgc agcgcctcac gcacgaagtc gatggtgatc gcccgtccgg
    38701 taaagttggc gttagcaata acgcggttca gcgccccttc cagctcacgt acgttagatc
    38761 gtagacgctt ggcgataaag aacgccactt cacccggcaa acgaatgtca ttttcgtcgg
    38821 cctttttcat caggatcgcc acacgggttt ccagctctgg cggttcgatc gccacagtca
    38881 gtccccaacc gaagcgggat ttcaaacgat cctcaacgcc gttgatctct ttcggatagc
    38941 gatccgaggt gagaatgatc tgttgattac cttccagcag ggcgttgaag gtgtggaaaa
    39001 actcttcctg agatcgttct ttattagcaa aaaactgaat atcgtcgatc agcagtgcat
    39061 ctacggaacg gtagtagcgt ttaaactctt cgatcgcgtt gttttgcagg gctttaacca
    39121 tgtcctgaac aaagcgctcg gagtgcatat aaaccacttt ggcattcggc ttgcgcgcca
    39181 taatgccgtt acccaccgca tgcagcaggt gagttttacc cagacccgtg ccgccataaa
    39241 ggaacaacgg gttataggca ccgccagggt tatccgccac ctggcaagcc gccgcgcgcg
    39301 ccagttggtt agatttacct tcaacgaagt tatcaaacgt gtgtttgacg tttacgttag
    39361 aacgataggt cggttctgcc ggggccggga cgttatccca acctgagcgc gtagaaggcg
    39421 cagcacgttg cggctgcgtt tgcgccacct gtgcaggggc cgcgacgttg ctcgtcactg
    39481 ccgcttgtgg cgtttgcgtc accggtttgg tgccgacttc aaaacgcagc tgtggggcat
    39541 ccgctccgca gaaactggtt agcagtccat tgatattatt aaggtacttg tcccgtaccc
    39601 aatcgaggac aaaacggttt ggcgcgtaca gggccagcgt gttatcgctc agttccgcct
    39661 gcaatgggcg tatccacata ctgaattctg tggctggtaa ctcatcctgc aatcgggcaa
    39721 gacactgctg ccaaagcgaa agtgacacgg cggactccac tcgaacaaaa gtcgataatg
    39781 actaaggctg aaacattcat gattgttgac gtacgtcgaa aagaccctgc ttgagggtga
    39841 cgcacgaacc gctgtctgcg gttatatgcc cgatcaagat cctgcaaaac gatcgggacc
    39901 gcggatcata gcctaaactg cgcaagagat cttctgtttc tcacagattt tccctattta
    39961 tccacaggac tttccagaac tcgctaagtg taaacgatcc tgccgcgagg cgggcacgat
    40021 ttacgccgca tattggaaaa tttaatgacc atagacaaaa attggcttaa tcgatctaat
    40081 aaagatccag gacgatcctt gcgctttacc catcagcccg tataatcctc cacccggcgc
    40141 gccatgctgg tttccactgg tgtgaggtcg tacattttcc ctgcgaaaag gtgcggaaaa
    40201 gcgcggtaaa taaggaaaga gaattgactc cggtgtgtac aattattaca atccggcctc
    40261 tttaatcacc catggcttcg gtgtccatcg tttcattttt cggcggatat ccaataaagc
    40321 cattgaattt attcaagttt aggtagaaat cgccatgaaa cgcacttttc aaccgtctgt
    40381 actgaagcgc aaccgttctc acggcttccg tgctcgtatg gctactaaaa atggtcgtca
    40441 ggttctggca cgtcgtcgtg ctaaaggccg cgctcgtctg accgtttcta agtaataaag
    40501 ctaacccctg agtggttaag ctcgcatttc ccagggagtt acgcttgtta actcccagtc
    40561 aattcacatt cgtcttccag cagccacaac gggctggcac gccgcaaatt accattctcg
    40621 gccgcctgaa ttcgctgggg catccccgta tcggtcttac agtcgccaag aaaaacgttc
    40681 gacgcgccca tgaacgcaat cggattaaac gcctgacgcg tgaaagcttc cgtctgcgcc
    40741 aacatgaact cccggctatg gatttcgtgg tggtggcgaa aaaaggggtt gccgacctcg
    40801 ataaccgtgc tctctcggaa gcgttggaaa aattatggcg ccgccactgt cgcctggctc
    40861 gcgggtcctg atagccctca ttcgggtcta tcaacgcctg attagtccgc tactcgggcc
    40921 gcattgtcgt ttcactccaa cctgttcaag ctacggaatt gaggcattgc gcaggtttgg
    40981 agtgataaaa ggcagttggt tgacggtgaa acgcgtatta aaatgccacc ctttacaccc
    41041 tggtggtgac gatcccgtcc cgcccggacc atttgatacc agagaacact aacgatggat
    41101 tcgcaacgca atcttttagt catcgctttg ctgttcgtgt ctttcatgat ctggcaagcc
    41161 tgggagcagg ataaaaaccc gcaacctcag gcccaacaga ccacgcagac aacgaccacc
    41221 gcagcgggta gcgccgccga ccagggcgta ccggccagtg gccaggggaa actgatctcg
    41281 gttaagaccg acgtgcttga tctgaccatc aacacccgtg gtggtgatgt tgagcaagct
    41341 ctgctgcctg cttacccgaa agagctgaac tctacccagc cgttccagct gttggaaact
    41401 tcaccgcagt ttatttatca ggcacagagc ggtctgaccg gtcgtgatgg cccggataac
    41461 ccggctaacg gcccgcgtcc gctgtataac gttgaaaaag acgcttatgt gctggctgaa
    41521 ggtcaaaacg aactgcaggt gccgatgacg tataccgacg cggcaggcaa cacgtttacc
    41581 aaaacgtttg tcctgaaacg tggtgattac gctgtcaacg tcaactacaa cgtgcagaac
    41641 gctggcgaga aaccgctgga aatctcctcg tttggtcagt tgaagcaatc catcactctg
    41701 ccaccgcatc tcgataccgg aagcagcaac ttcgcactgc acaccttccg tggcgcggcg
    41761 tactccacgc ctgacgagaa gtatgagaaa tacaagttcg ataccattgc cgataacgaa
    41821 aacctgaaca tctcttcgaa aggtggttgg gtggcgatgc tgcaacagta tttcgcgacg
    41881 gcgtggatcc cgcataacga cggtaccaac aacttctata ccgctaatct gggtaacggc
    41941 atcgtcgcta tcggctataa atctcagccg gtactggttc agcctggtca gactggcgcg
    42001 atgaacagca ccctgtgggt tggcccggaa atccaggaca aaatggcagc tgttgctccg
    42061 cacctggatc tgaccgttga ttacggttgg ttgtggttca tctctcagcc gctgttcaaa
    42121 ctgctgaaat ggatccatag ctttgtgggt aactggggct tctccattat catcatcacc
    42181 tttatcgttc gtggcatcat gtacccgctg accaaagcgc agtacacctc catggcgaag
    42241 atgcgtatgc tgcagccgaa gattcaggca atgcgtgagc gtctgggcga tgacaaacag
    42301 cgtatcagtc aggaaatgat ggcgctgtac aaagctgaga aggttaaccc gctgggcggc
    42361 tgcttcccgc tgctgatcca gatgccaatc ttcctggcgt tgtactacat gctgatgggt
    42421 tccgttgaac tgcgtcaggc accgtttgca ctgtggatcc acgacctgtc ggcacaggac
    42481 ccgtactaca tcctgccgat cctgatgggc gtaacgatgt tcttcattca gaagatgtcg
    42541 ccgaccactg tgaccgaccc gatgcagcag aagatcatga cctttatgcc ggtcatcttc
    42601 accgtgttct tcctgtggtt cccgtcaggt ctggtactgt actatatcgt cagcaacctg
    42661 gtaaccatta ttcagcagca gctgatttac cgtggtctgg aaaaacgtgg cctgcatagc
    42721 cgcgagaaga aaaaatcctg attcggtgag ttttcgctaa aataagggcg gtcagttgac
    42781 cgcctttttt cttttcgtag ggcggataag caccgcgcat ccgccacaca aagcaacagg
    42841 aacatcatga gcgataatga cactatcgta gcccaggcca cgcctccggg acgtggcggc
    42901 gttggcatcc tgcgcatctc cggcttcaaa gcccgtgaag ttgccgaaac cgtgctgggt
    42961 aaactgccta agccgcgcta cgccgattat cttccgttta aagacgccga cggcagcgtg
    43021 ctcgatcagg ggattgcgct atggttccct ggcccgaact cgttcaccgg cgaagatgtg
    43081 ctggaactgc aaggtcatgg cggtccggtg atcctcgacc tgctgttaaa acgcattctg
    43141 accattcccg gcctgcggat tgctcgccct ggtgagtttt ccgaacgcgc gtttcttaac
    43201 gataaacttg acttagccca ggccgaggcg attgccgatc ttatcgacgc cagttcggaa
    43261 caggcggccc gttcggcact taactcgctg caaggcgcat tctccgcacg ggttaatcat
    43321 ctggtagaag ccctcaccca cttgcgcatt tacgtcgaag cggcaattga tttccctgat
    43381 gaagagatcg atttcctctc cgacggcaaa attgaagccc agcttaatga cgtgatggcc
    43441 gatctcgatg cagtgcgtgc tgaagcacgt cagggtagtt tgttgcgcga agggatgaaa
    43501 gtagtgattg ccggacgtcc taatgccggt aaatcgagcc tgttaaacgc gctggcgggc
    43561 cgtgaagcgg caatcgtaac cgatatcgcc ggaaccacgc gtgacgtgct gcgtgagcat
    43621 atccacattg acggaatgcc gctgcatatc atcgacactg ccgggctacg tgaggccagt
    43681 gacgaagtgg aacgtattgg tatcgagcgc gcgtggcagg aaattgaaca ggccgaccgc
    43741 gtgctgttta tggtcgatgg caccacgaca gacgccgttg atccggcaga gatctggccg
    43801 gaatttatcg cccgtctgcc cgcgaaactg ccgatcaccg tggtacgcaa taaagccgat
    43861 atcaccggcg aaacgctggg aatgagtgaa gtgaacggtc acgcgttaat tcgtctctcg
    43921 gcaaggactg gtgaaggcgt ggacgtgctg cgtaaccatc tcaaacagag catgggcttt
    43981 gacaccaata tggaaggcgg cttcctggcg cgccgtcgcc acttacaggc gctggaacag
    44041 gcagcggagc atttgcagca aggtaaagcg caactgttgg gagcctgggc aggtgaactg
    44101 ctggcggaag agttgcgcct ggcacagcag aacttaagcg aaatcaccgg ggaatttact
    44161 tcagacgacc tgctggggcg gattttctcc agcttctgta ttggtaagta accgcgctta
    44221 cgaagccgta ttctgactgt cagatgcggc ttcgcttcat tgttagcact cctgttatgc
    44281 ctcaaccc
//
LOCUS       DAZOYF010000015        41675 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZOYF010000015 DAZOYF010000000
VERSION     DAZOYF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.015"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(23..967)
                     /gene="leuO"
                     /locus_tag="ACHUOF_000813"
     CDS             complement(23..967)
                     /gene="leuO"
                     /locus_tag="ACHUOF_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR0610107.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            1626..1712
                     /gene="leuL"
                     /locus_tag="ACHUOF_000814"
     CDS             1626..1712
                     /gene="leuL"
                     /locus_tag="ACHUOF_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR0610108.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            1805..3376
                     /gene="leuA"
                     /locus_tag="ACHUOF_000815"
     CDS             1805..3376
                     /gene="leuA"
                     /locus_tag="ACHUOF_000815"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR0610109.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            3376..4467
                     /gene="leuB"
                     /locus_tag="ACHUOF_000816"
     CDS             3376..4467
                     /gene="leuB"
                     /locus_tag="ACHUOF_000816"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR0610110.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            4470..5870
                     /gene="leuC"
                     /locus_tag="ACHUOF_000817"
     CDS             4470..5870
                     /gene="leuC"
                     /locus_tag="ACHUOF_000817"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR0610111.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            5881..6486
                     /gene="leuD"
                     /locus_tag="ACHUOF_000818"
     CDS             5881..6486
                     /gene="leuD"
                     /locus_tag="ACHUOF_000818"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR0610112.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(6535..7713)
                     /gene="setA"
                     /locus_tag="ACHUOF_000819"
     CDS             complement(6535..7713)
                     /gene="setA"
                     /locus_tag="ACHUOF_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR0610113.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(7815..7946)
                     /gene="sgrT"
                     /locus_tag="ACHUOF_000820"
     CDS             complement(7815..7946)
                     /gene="sgrT"
                     /locus_tag="ACHUOF_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR0610114.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            8035..9690
                     /gene="sgrR"
                     /locus_tag="ACHUOF_000821"
     CDS             8035..9690
                     /gene="sgrR"
                     /locus_tag="ACHUOF_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR0610115.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      9724..9823
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9854..10837
                     /gene="thiB"
                     /locus_tag="ACHUOF_000822"
     CDS             9854..10837
                     /gene="thiB"
                     /locus_tag="ACHUOF_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR0610116.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            10813..12423
                     /gene="thiP"
                     /locus_tag="ACHUOF_000823"
     CDS             10813..12423
                     /gene="thiP"
                     /locus_tag="ACHUOF_000823"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR0610117.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            12407..13105
                     /gene="thiQ"
                     /locus_tag="ACHUOF_000824"
     CDS             12407..13105
                     /gene="thiQ"
                     /locus_tag="ACHUOF_000824"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR0610118.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(13219..13983)
                     /locus_tag="ACHUOF_000825"
     CDS             complement(13219..13983)
                     /locus_tag="ACHUOF_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0610119.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(14069..14947)
                     /gene="araC"
                     /locus_tag="ACHUOF_000826"
     CDS             complement(14069..14947)
                     /gene="araC"
                     /locus_tag="ACHUOF_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR0610120.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            15286..16986
                     /gene="araB"
                     /locus_tag="ACHUOF_000827"
     CDS             15286..16986
                     /gene="araB"
                     /locus_tag="ACHUOF_000827"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR0610121.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            16997..18499
                     /gene="araA"
                     /locus_tag="ACHUOF_000828"
     CDS             16997..18499
                     /gene="araA"
                     /locus_tag="ACHUOF_000828"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR0610122.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            18699..19394
                     /gene="araD"
                     /locus_tag="ACHUOF_000829"
     CDS             18699..19394
                     /gene="araD"
                     /locus_tag="ACHUOF_000829"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR0610123.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLHKH
                     YLRKHGAKAYYGQ"
     gene            19469..21820
                     /gene="polB"
                     /locus_tag="ACHUOF_000830"
     CDS             19469..21820
                     /gene="polB"
                     /locus_tag="ACHUOF_000830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR0610124.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            21985..24891
                     /gene="rapA"
                     /locus_tag="ACHUOF_000831"
     CDS             21985..24891
                     /gene="rapA"
                     /locus_tag="ACHUOF_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR0610125.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            24903..25562
                     /gene="rluA"
                     /locus_tag="ACHUOF_000832"
     CDS             24903..25562
                     /gene="rluA"
                     /locus_tag="ACHUOF_000832"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR0610126.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(25679..26494)
                     /gene="djlA"
                     /locus_tag="ACHUOF_000833"
     CDS             complement(25679..26494)
                     /gene="djlA"
                     /locus_tag="ACHUOF_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR0610127.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            26749..29103
                     /gene="lptD"
                     /locus_tag="ACHUOF_000834"
     CDS             26749..29103
                     /gene="lptD"
                     /locus_tag="ACHUOF_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR0610128.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            29156..30442
                     /gene="surA"
                     /locus_tag="ACHUOF_000835"
     CDS             29156..30442
                     /gene="surA"
                     /locus_tag="ACHUOF_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR0610129.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            30442..31431
                     /gene="pdxA"
                     /locus_tag="ACHUOF_000836"
     CDS             30442..31431
                     /gene="pdxA"
                     /locus_tag="ACHUOF_000836"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR0610130.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            31428..32249
                     /gene="rsmA"
                     /locus_tag="ACHUOF_000837"
     CDS             31428..32249
                     /gene="rsmA"
                     /locus_tag="ACHUOF_000837"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR0610131.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            32252..32629
                     /gene="apaG"
                     /locus_tag="ACHUOF_000838"
     CDS             32252..32629
                     /gene="apaG"
                     /locus_tag="ACHUOF_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR0610132.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            32636..33478
                     /gene="apaH"
                     /locus_tag="ACHUOF_000839"
     CDS             32636..33478
                     /gene="apaH"
                     /locus_tag="ACHUOF_000839"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR0610133.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(33556..34035)
                     /gene="folA"
                     /locus_tag="ACHUOF_000840"
     CDS             complement(33556..34035)
                     /gene="folA"
                     /locus_tag="ACHUOF_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR0610134.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34226..36088)
                     /gene="kefC"
                     /locus_tag="ACHUOF_000841"
     CDS             complement(34226..36088)
                     /gene="kefC"
                     /locus_tag="ACHUOF_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR0610135.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWVKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36081..36611)
                     /gene="kefF"
                     /locus_tag="ACHUOF_000842"
     CDS             complement(36081..36611)
                     /gene="kefF"
                     /locus_tag="ACHUOF_000842"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR0610136.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36659..38053)
                     /locus_tag="ACHUOF_000843"
     CDS             complement(36659..38053)
                     /locus_tag="ACHUOF_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0610137.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(38112..38399)
                     /gene="fixX"
                     /locus_tag="ACHUOF_000844"
     CDS             complement(38112..38399)
                     /gene="fixX"
                     /locus_tag="ACHUOF_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR0610138.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38396..39682)
                     /locus_tag="ACHUOF_000845"
     CDS             complement(38396..39682)
                     /locus_tag="ACHUOF_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0610139.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39733..40674)
                     /locus_tag="ACHUOF_000846"
                     /pseudo
     CDS             complement(39733..40674)
                     /locus_tag="ACHUOF_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(40689..41459)
                     /locus_tag="ACHUOF_000847"
     CDS             complement(40689..41459)
                     /locus_tag="ACHUOF_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR0610140.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        10132 a        11432 c        10833 g         9278 t
ORIGIN      
        1 gtctggccta ttctgcattc agttagcgtt tgcaaattga gactaattgt tcttccatcc
       61 actgatggcc tttatcgcgc ccggcagctt catgccaaga gagataacag gttctgctgt
      121 tttgttttaa cggcagcggt aatacctgta attctaagga ttcagcgaac tcttcagcca
      181 gccaacgcgg cgcaatagcg accaaatgcg tttgcgacac cacgctaagt acgctcatca
      241 ttgccatgcc ctgatacgcg atactggctt gcttatctac cgtgtcatac caaggttgac
      301 taaatgacgc gaaacgatcg agcgaaaccg ccgcatgttg ttcgttataa acatcatgtt
      361 tcagtaacgg gcctttaatt gttggatgat ttttgctggc taccagcacc atttcatctt
      421 taaataatgg cacgctggta aattcaggac gatggaagtc ttcataacta atcacaaact
      481 ccgtttcctg ataacgcagc tgatgttcag tgttctgatt taatgaagac ttgaacataa
      541 catgtatatt tggcgcaatc tgctcaatgt gattataaat ctgcgaggtc agaatgctgt
      601 ctaacgggct gcaaacacaa agatgaaata cacgttcact gctcgcgggt tcaaaacctg
      661 aaccaggcaa ttcattttgt actagttgca atgcctggcg aactgaacca aaaagttgaa
      721 atgcgcgagc agtcggttga ataccacggc cataacgaac aaaaagctcg tcattaaaca
      781 tcaccttcag gcgtgcaaca gcgttactga ccgcaggttg cgacataccc agaacatgag
      841 cggcacgggt aatgttttgc tcctgcatca cggcatcgaa aacggttaat aagttgagat
      901 cgaccatgcg tagctgtggc ttgcttaact ccgccgtctc tgaatgatct gtttgtacct
      961 ctggcatact taactccact gtcacactta actccctttc ccttattgga atgcagagaa
     1021 ataatcctga agaatatgat ttatcatgca tataaaataa gaaaaagcaa aatgagtaaa
     1081 attcggaaaa cataaagata cgggttctga taaaaacaga atccattgcc gtggaatgag
     1141 tcatttacca cagcataata atccataatg ttttgttgcg aaaacaatct aatcataact
     1201 accgcgaata ctcaatcatc tacaaaatgg attaaatgtg cttatttaat aattaattta
     1261 tatatttaat gcattaattc ttaacattaa ttgttcaata atattcacca aatcaatatc
     1321 aaaaaaaatc gcaaaacata taattcaata caaatcatca ggataggttt tgcaacgcgt
     1381 gcattttgtc ccctttttcc tcgttgatta gatgcaaaaa tttatgctga aatatgtcaa
     1441 ccgatgaaaa gcgtcggtag ttaagcagaa attaatatcg cttactttaa ccaccgtagc
     1501 acaattagct aattttacgg atgcagaact cacgctggcg ggacgttttt attgcgtcag
     1561 ggttgacatc cgtttttgta tccagtaact ctaaaagcat atcgcattca tctggagctg
     1621 atttaatgac tcacatcgtt cgctttatcg gtctactact actaaacgca tcttctttgc
     1681 gcggtagacg agtgagcggc atccagcatt aagccagcac gcagtcaaac aaaaaacccg
     1741 cgccattgcg cgggtttttt tatgcccgaa gcgaggcgct ctaaaagaga caaggaccca
     1801 aaccatgagc cagcaagtca ttattttcga taccacattg cgcgacggtg aacaggcgtt
     1861 acaggcaagc ttgagtgtga aagaaaaact gcaaattgcg ctggcccttg agcgtatggg
     1921 tgttgacgtg atggaagtcg gtttccccgt ctcttcgccg ggcgattttg aatcggtgca
     1981 aaccatcgcc cgccaggtta aaaacagccg cgtatgtgcg ttagctcgct gcgtggaaaa
     2041 agatatcgac gtggcggccg aatccctgaa agtcgccgaa gccttccgta ttcatacctt
     2101 tattgctacc tccccaatgc acatcgccac caagctgcgc agcaccctag acgaggtgat
     2161 cgaacgcgct atctatatgg tgaaacgcgc ccgtaattac accgatgatg ttgaattttc
     2221 ttgcgaagat gccgggcgta cacccattgc cgatctggcg cgagtggtcg aagcggcgat
     2281 taatgccggt gccaccacca tcaacattcc ggacaccgtg ggctacacca tgccgtttga
     2341 gttcgccgga atcatcagcg gcctgtatga acgcgtacct aacatcgaca aagccattat
     2401 ttccgtacat acccacgacg atttaggcct ggcggtcggc aactcactgg cggcggtaca
     2461 tgccggtgca cgccaggtgg aaggtgcaat gaacgggatc ggcgagcgtg ccggtaactg
     2521 ttcgctggaa gaagtcatca tggcgattaa agttcgtaag gatattctca acgtccacac
     2581 cgccattaat caccaagaga tatggcgcac cagccagtta gttagccaga tttgtaatat
     2641 gccgatcccg gcaaacaaag ccattgttgg cagcggcgca ttcgctcact cctccggtat
     2701 ccaccaggat ggcgtgctga aaaaccgcga aaactacgaa atcatgacac cagaatctat
     2761 tggtctgaac caaatccagc tgaatctgac ctctcgttcg gggcgtgcgg cggtgaaaca
     2821 tcgcatggat gagatggggt ataaagaaag tgaatataat ttagacaatt tgtacgatgc
     2881 tttcctgaag ctggcggaca aaaaaggtca ggtgtttgat tacgatctgg aggcgctggc
     2941 cttcatcggt aagcagcaag aagagccgga gcatttccgt ctggattact tcagcgtgca
     3001 gtctggctct aacgatatcg ccaccgccgc cgtcaaactg gcctgtggcg aagaagtcaa
     3061 agcagaagcc gccaacggta acggtccggt cgatgccgtc tatcaggcaa ttaaccgcat
     3121 cactgactat aacgtcgaac tggtgaaata cagcctgacc gccaaaggtc acggtaaaga
     3181 tgcgctgggt caggtggata ttgtcaccaa ctacaacggt cgccgcttcc acggcgtcgg
     3241 cctggccacc gatattgtcg agtcctccgc caaagccatg gtgcacgtac tgaacaatat
     3301 ctggcgtgcc gcagaagtcg aaaaagagtt gcaacgcaaa actcaacaca acgaaaacaa
     3361 caaggaaacc gtgtgatgtc gaagaattac catattgccg tattgccggg agacggtatt
     3421 ggtccggaag tgatgaccca ggcgctgaaa gtgctgaatg ccgtgcgcaa ccgctttgcg
     3481 atgcgcatca ccaccagcca ttacgatgta ggcggcgcag ccattgataa ccacgggcaa
     3541 ccactgccgc ctgcgacggt tgaaggttgt gagcaagccg atgccgtgct gtttggctcg
     3601 gtaggcggcc cgaagtggga acatttacca ccagaccagc aaccagaacg cggcgcgctg
     3661 ctgccgctgc gtaagcactt caaattattc agcaacctgc gcccggcaaa actgtatcag
     3721 gggctggaag cattctgtcc gctgcgtgca gacattgccg ctaacggctt cgacatccag
     3781 tgcgtgcgcg aactgaccgg cggcatctat ttcggtcagc caaaaggccg cgaaggtagc
     3841 ggacaatatg aaaaagcgtt tgataccgag gtgtatcacc gttttgagat cgagcgtatt
     3901 gcccgcatcg cgtttgaatc tgctcgtaaa cgtcgccaca aagtcacctc aatcgacaaa
     3961 gctaacgtgc tgcaatcctc tattttatgg cgtgaaatcg tcaacgagat cgccacggaa
     4021 tacccggatg tcgaactggc gcatatgtac atcgacaacg ccaccatgca gctgattaaa
     4081 gatccatcac agtttgacgt tctgctgtgc tccaacctgt ttggcgacat tctgtctgac
     4141 gagtgcgcaa tgatcactgg ctcgatgggc atgttgcctt ccgccagcct gaacgagcaa
     4201 ggttttggac tgtatgaacc ggcgggcggc tcggcaccag atatcgcagg caaaaacatc
     4261 gccaacccga ttgcacaaat cctgtcgctg gcactgctgc tgcgttacag cctggatgcc
     4321 gatgatgcgg cttccgccat tgaacgcgcc attaaccgtg cattagaaga aggcattcgc
     4381 accggggatt tagcccgtgg cgctgccgcc gttagtaccg atgaaatggg cgatatcatt
     4441 gcccgctatg tggcagaagg ggtgtaatca tggctaaaac gttatacgaa aaattattcg
     4501 acgctcacgt agtgtacgaa gccgaaaacg aaaccccgct gttatatatc gaccgccacc
     4561 tggtgcatga agtgacctca ccgcaggcgt tcgatggtct gcgcgcccac ggtcgcccgg
     4621 tacgtcagcc gggcaaaacc ttcgccacta tggatcacaa cgtctctacc cagaccaaag
     4681 acattaatgc ctgtggtgaa atggcgcgca tccagatgca ggagctgatt aaaaactgca
     4741 aagaatttgg cgtcgagctg tatgacctga atcacccgta tcaggggatc gtccacgtaa
     4801 tggggccgga acagggcgtc accttgccgg ggatgaccat tgtctgcggc gactcgcata
     4861 ccgccaccca cggcgcgttt ggcgcactgg cctttggtat cggcacttcc gaagttgaac
     4921 acgtactggc aacacaaacc ctgaaacagg gccgtgcgaa gaccatgaaa attgaagtcc
     4981 agggcaaagc cgcgccgggc attaccgcaa aagatatcgt gctggcaatt atcggtaaaa
     5041 ctggcagcgc aggcggcacc gggcatgtgg tggagttttg cggcgaagca attcgtgatt
     5101 taagcatgga aggtcgtatg accctgtgca atatggcgat cgaaatgggc gcaaaagccg
     5161 ctctggttgc tcctgacgaa accaccttta actatgtcaa aggccgtctg catgcgccga
     5221 aaggcaaaga tttcgacgac gccgttgcct actggaaaac cctgcaaacc gacgaaggcg
     5281 caactttcga taccgttgtc actctgcaag cagaagaaat ttcaccgcag gtcacctggg
     5341 gcaccaatcc cggccaggtg atttctgtga acgacaatat tcccgatccg gcttcgtttg
     5401 ccgatccggt tgaacgtgcg tcggcagaaa aagcgctggc ctatatgggg ctgaaaccgg
     5461 gtattccgct gaccgaagtg gctatcgaca aagtgtttat cggttcctgt accaactctc
     5521 gcattgaaga tttacgcgcg gcagcggaga tcgccaaagg gcgaaaagtc gcgccaggcg
     5581 tgcaggcact ggtggttccc ggctctggcc cggtaaaagc ccaggcggaa gcggaaggtc
     5641 tggataaaat atttattgaa gccggttttg aatggcgctt gcctggctgc tcaatgtgtc
     5701 tggcgatgaa caacgaccgt ctgaatccgg gcgaacgttg tgcatccacc agcaaccgta
     5761 actttgaagg ccgccagggg cgcggcgggc gcacgcatct ggtcagcccg gcaatggctg
     5821 ccgctgctgc tgtgaccgga catttcgccg acattcgcaa cattaaataa ggagcacacc
     5881 atggcagaga aatttatcaa acacacaggc ctggtggttc cgctggatgc cgccaatgtc
     5941 gataccgatg caatcatccc gaaacagttt ttgcagaaag tgacccgtac gggttttggc
     6001 gcgcatctgt ttaacgactg gcgttttctc gatgaaaaag gccaacagcc aaacccggac
     6061 ttcgtgctga acttcccgca gtatcagggc gcttccattt tgctggcacg agaaaacttc
     6121 ggctgtggct cctcgcgtga gcacgcgccc tgggcattga ccgactacgg ttttaaagtg
     6181 gtgattgcgc cgagttttgc tgacatcttc tacggcaata gctttaacaa ccagctgctg
     6241 ccggtgaaat taagcgatgc agaagtggac gaactgtttg cgctggtgaa agccaatccg
     6301 gggatccatt tcgacgtgga tctggaagcg caagaggtga aagcgggaga gaaaacctat
     6361 cgctttacca tcgatgcctt ccgccgccac tgcatgatga atggtctgga cagcatcggc
     6421 ctgaccctgc agcacgacga cgctattgcg tcttatgaag caaaacaacc tgcgtttatg
     6481 aattagaccc cttgcccggt caaatgaccg ggctttccgc tatcgtccac gtcatcaaac
     6541 gtctttaacc tttgcggtta aaaataatgc gacaacagaa ataatcgcaa ttacccagta
     6601 gacagcaaag tgcccccaac tttgtgcaat tgctccctga ataacaccag ccagaattac
     6661 cccggtagag atagtgttgg taaataaggt ggtagctgcc cccgctcttc caggcattaa
     6721 atcctgaaac catagcatcc caatacccgc aacaatgccg ataaatacag cgttaaaaag
     6781 ttgcagcgtc atcaacgcca tacggctatg aaagaaaatc aatccagtgt aaaacagcac
     6841 tcctgccacc actgcaatga ccatcattcg ccgcttacca taacgtttga catagtagcc
     6901 agccagaatc attgctggta tttccagtcc agctgccgtc cccatcagga aacccgccag
     6961 tttgtctggc aatcctaaat cgctactgat ccacaatggc atatcaataa tgtacatagt
     7021 gttgcaggtc cacattaacg tcgaggcgac aaataacatc cgtacgttac tatcctgcca
     7081 gtcaccttgc attgataaag cattttccga cggcagttct acccgcgcca cagacagaag
     7141 cataaatgca atcaatacca gactgagtgt gaatatcccg gcggcaatcg aaaacatcac
     7201 tgtaaagccg taattcaacg ccagcataaa ggccagcggt ggaccgatta cccatgccag
     7261 agaaagctgc gcacgcatca ccgagctaaa catcaccact tctcgcgccg agttatccgc
     7321 gtattcccgc gccagagcaa ataactgtgg cattgccgta ttggccagag atgccagaag
     7381 cacaccacag gtgataagcg tcagataatg acgattaaat gcaaacagta gcgcattgcc
     7441 aatagccatc aaacagcaaa atataatcag ttttcgccga tcgccctgac tgtcagaacg
     7501 ttttgccaac cagaggctta cgccgatccc agcaatagca ttcaccgtat aaaagagtcc
     7561 gatccagaaa ggttgcgcgc caacctcacg actcagaaat aagctcaatg taggagcctg
     7621 tagcgccccg gccaccccca tcataaaagc gaccagcata aatgccgcgt aaacaccgtt
     7681 catacggcga gccatcgtca ttatccagat catacgttcc ctttttagcg cggcgagaat
     7741 aaaaaaaacc agcaggtata atctgctggc gggtgatttt acaccaatac tcagtcacac
     7801 atgatgcagg caagtcaact ttcagaattg cggtcatccc actgcatcag tccttcaagc
     7861 atggttaatc gttgtgggac gcttaaccaa cgcaaccagc acaacttcgc tgtcgcggta
     7921 aaatagtgct gataaaactg acgcatgggg cacctccttg cttcatcgtt aggaattatt
     7981 gacttaatat agggaaaata aaattgctgt cttttgcgca ggagttcccc ttttatgcca
     8041 tctgctcgtc tgcaacaaca gttcatccgc ctgtggcaat gctgcgaggg taaatcgcag
     8101 gacacaacgc tcaacgaact ggcagcgtta ttgagctgct cgcgtcgtca tatgcgtacc
     8161 ctgctcaaca ccatgcagga tcgcggctgg ctgacgtggg aagcggaagt cgggcgcggt
     8221 aaacgctcgc gtctgacatt cctctatacc gggctggcgc ttcagcaaca gcgggcggaa
     8281 gacctgctgg agcaggatcg tatcgaccaa ctagtgcagt tggttggcga caaagcgact
     8341 gtgcggcaaa tgctggtttc tcatctgggc cgcagcttcc gccaggggcg gcacatcctg
     8401 cgcgtgctct actatcgtcc gttgcgtaat ctgctacctg gcagcgcatt gcgccgttcc
     8461 gaaacccata ttgcccggca aatcttcagt tcgctaacgc gcataaatga ggaaaatggg
     8521 gaactggaag cagacatcgc ccaccactgg cagcaaatat caccgcttca ctggcgtttc
     8581 tttttgcgtc caggagtcca ttttcatcat ggtcgtgaac tggaaatgga cgacgtgatc
     8641 gcctctttaa aacgaatcaa tacgctgccg ctctattcgc atattacgga cattgtgtcg
     8701 ccgacgccct ggacgctgga tatccatctc acgcagccgg accgctggtt gccgttactg
     8761 ctggggcaag ttccggcgat gatcctgccg tgcgaatggg aaaccctcag taactttgcc
     8821 agccatccta ttggcaccgg tccgtatgcg gtgattcgca acagcaccaa tcaactgaaa
     8881 attcaggcat tcgatgactt cttcggttac cgggcattaa tcgacgaagt taacgtctgg
     8941 gttctgccgg aaattgccga cgagccagcc ggtggtctga tgctaaaagg tccacagggc
     9001 gagaaaaaag agattgaaag ccgcctggag gaaggttgct actatttact gttcgacagc
     9061 cgcacccatc gcggggcgaa tcagcaagtc agggactggg taagctatgt gctttctcca
     9121 actaatctgg tctatttcgc tgaggaacag taccagcaac tgtggttccc ggcttatgga
     9181 ctgctccccc gttggcacca tgcccgcacc ataaagagcg aaaaaccggc tggcctggaa
     9241 agcctcaccc tgacctttta tcaggatcac agtgagcatc gggtgattgc cgggatcatg
     9301 cagcagattc tggcaagtca ccaggtcacg ctggaaatca aagagatcag ctacgatcag
     9361 tggcatgaag gagagatcga gagcgatatc tggcttaata gcgccaactt tacgctgccg
     9421 ctggattttt cgctgttcgc gcacttgtgc gaggtgccgc tgctccaaca ctgtattccg
     9481 atcgactggc aagccgacgc cgcccgctgg cgcaatggcg aaatgaacct ggcgaactgg
     9541 tgccagcaac tggtcgccag taaagcaatg gtgccgctta tccaccactg gctgatcatt
     9601 cagggacaac gcagtatgcg cggcctgcgt atgaataccc tcggctggtt cgattttaaa
     9661 tcagcgtggt ttgcgccacc ggatccatga ttgctggtag ataacaaaat cactacacta
     9721 acgccgttct caacggggtg ccacgcaaac gcgtgcgctg agaaaatacc cgtcgaacct
     9781 gatccggata acgccggcga agggatttga ggctccttct caagtccttt gccactcttt
     9841 tttgaggtgc aaagtgttaa aaaaatgtct tcccctgctg ttgctgtgca cagcgcccgt
     9901 tttcgctaaa cccgttctga ctgtttatac ctacgattcc ttcgccgccg actgggggcc
     9961 tggtccaaag atcaaaaaag cttttgaagc caactgtaac tgcgaactga aactggtggc
    10021 gctggaagat ggcgtttcgc ttctcaaccg tctacggatg gaaggcaaaa atagtaaagc
    10081 cgatgtggtg ctggggctgg ataacaatct gttagacgcc gccagtaaaa ccggactatt
    10141 tgccaaaagc ggtgtggcag cggatgccgt taacgttccc ggcggctgga ataatgacac
    10201 tttcgtcccg tttgattacg gctatttcgc cttcgtctat gacaagaaca aactgaaaaa
    10261 cccgccgcaa agcctgaaag aattggttga gagcgatcag aactggcggg tgatttatca
    10321 ggatccgcgt accagcacgc cggggctggg tctgctgttg tggatgcaaa aagtctatgg
    10381 cgatgacgcc ccacaagcct ggcaaaaact ggcgaagaaa accgtcacgg tcaccaaagg
    10441 ctggagcgaa gcctacggcc tgtttttaaa aggtgaaagc gatctggtac tgagttacac
    10501 cacctctccg gcttatcaca ttctcgaaga gaaaaaagat aactacgccg ccgcgaactt
    10561 tagcgaaggt cactatctgc aggtggaagt cgccgcccgc accgctgcca gcaagcagcc
    10621 ggagctggcg caaaaattcc tccagtttat ggtttctccg gcttttcaga atgcgatccc
    10681 aaccggtaac tggatgtatc cggtggcaaa tgtcacgctg cctgccgggt ttgaacaatt
    10741 gaccaaaccg gccaccacgc tggagttcac accagctgaa gtggcggcac aacgtcaggc
    10801 atggattagc gaatggcaac gcgccgtcag ccgttaattc ccggctggtt aattccaggt
    10861 gtaagcgccg ccacgctggt ggtagcggtt gcgctcgcgg cgtttctcgc cctgtggtgg
    10921 aacgcgccgc agggtaactg ggtggcagtc tggcaggaca gctacctgtg gcatgtggtg
    10981 cgcttctcct tctggcaggc gtttctctcg gcgctactct ctgtcgtacc cgcgatattc
    11041 ctcgcccgcg ccctctatcg caggcgcttt ccgggtcggc tggcgctgtt gcgtctgtgc
    11101 gcaatgacct tgatcctccc ggtgctagtt gctgttttcg gcattcttag cgtctatggt
    11161 cgtcagggct ggctggcatc gctctgccaa tcgctcggtc tggagtggac cttttcgccc
    11221 tacggcctgc aaggcatttt gctggcgcac gtatttttta atctgccgat ggcgagccgc
    11281 ttattactcc aggcactgga aaacattccc ggcgaacaac gtcagcttgc cgcccagctt
    11341 gggatgcgcg gctggcattt tttccgcttc gtcgaatggc cgtggctacg gcgacaaatc
    11401 ccgccggttg ccgcgcttat ctttatgctc tgtttcgcca gcttcgccac cgtgctatcg
    11461 ctgggcggcg gtccgcaggc aaccactatc gagctggcta tttatcaggc gctgagttac
    11521 gactacgatc ctgcccgcgc ggcgatgctg gcgctgctcc agatggtgtg ttgccttggg
    11581 ctggtgctgt tgagtcagcg actaagtaag gccattgcgc ccggcaccac gctgctgcaa
    11641 ggctggcgcg atccggacga tcgtctgcat agccgcattt gcgacacggt gttaatagtg
    11701 ctggcgctgc tgctgttgct gccaccgttg ctggcggtga tcgtcgatgg ggtaaatcgc
    11761 cagttgccgg aagtgctggc acaaccggtg ctgtggcagg cgctgtggac ctcgttgcgt
    11821 attgcgctgg cggcaggtgt attgtgcgta gtgctgacca tgatgctgct atggagcagt
    11881 cgcgaactgc gggcgcggca gaaaatgctg gcgggtcagg cgctggagat gagcggcatg
    11941 ttgatcctcg ccatgccggg gattgtgctg gctaccggct tctttttact gctcaacaac
    12001 actatcggcc tgccacaatc tgctgacggc attgtgattt tcaccaatgc gttaatggcg
    12061 atcccttatg cgctgaaagt actggaaaac ccgatgcgcg atatcaccgc ccgctacagt
    12121 atgttgtgtc agtcgctggg cattgaaggc tggtcgcgct taaaagtggt cgagctgcgc
    12181 gccctgaaac gtccactggc gcaggcgctg gcttttgcct gcgtgctgtc gattggtgat
    12241 tttggcgtag tggcgttgtt cggtaacgat gatttccgta ccctgccgtt ttatctctac
    12301 cagcaaattg gctcctatcg cagccaggac ggcgcggtca ccgcgttaat tctgctacta
    12361 ctctgttttc tgctgtttac cgtgattgaa aaactaccgg ggcgaaatgt taaaactgac
    12421 tgatatcacc tggctttatc accatttgcc gatgcgtttt agcttaacgg tagaacgcgg
    12481 cgagcaggtg gcaatcctcg ggccaagcgg cgcgggtaaa agtaccctgc tgaatttgat
    12541 tgccggtttt ctgacgccag ccagcggttc gctgactatc gatggtgttg atcacaccac
    12601 gacgccgccg tcacgccgtc cggtgtcgat gctgtttcag gagaacaacc tgttcagcca
    12661 tctgacggtc gcgcagaaca tcgggctggg gctaaatccg gggttgaaac tgaacgcggc
    12721 acagcaggag aaaatgcacg cgattgcccg tcagatgggg attgataatt taatggcgcg
    12781 gttaccgggc gagctttccg gcggtcagcg acagcgcgtg gcgttagcgc gttgtctggt
    12841 gcgcgaacag ccgattttat tgctcgatga accgttctct gcgctcgatc cggcgttacg
    12901 tcaggagatg ctgacgctgg tgagcacgag ctgccagcag caaaaaatga cgctattgat
    12961 ggtgtcgcac agtgtggaag atgcggcgcg gatcgccaca cgctcggtgg tggtcgccga
    13021 cgggcgcatc gcctggcagg gtaagactga agaattgttg agcggtaagg cgagtgcttc
    13081 ggcaatattg gggattacgg gttagtatgc actgtcagat gcggcgtgaa caccttatcc
    13141 gacctacgga cagtgcgttt tgtaggcctg ataagacgcg gtcagcgtcg catgaggcaa
    13201 cccgcacaag acaatcccct aaaccccaac cactttacgc aaaatatcga tatacaccgg
    13261 catcaacggg tggcgaatta acaccaccag cgccaccacg ccgatggcag aaatcaacgg
    13321 cgtcagccac aacaaacggc cgcgcgacaa ataatgactt aaacgatcgg ttgctttacc
    13381 gctgcgccat aaccgccagc acagccagcc accaacccac aaaaataccg ccgtcgccag
    13441 cagcaaccat ttaaactcgc cgctctgcat tccggctgga atatcgatcg ccgcgcccgc
    13501 cagaatccca ggcaggaagt aaaacggtgg ccacagcagg cagccgataa tattcggcgt
    13561 aatgaatttt gccaccggca gatccagcat tcccgccacc attggcacca gcggacgcgt
    13621 cgggccaaca aagcgaccga ccagaatggt gaacatgctg tgttgatgca acgcatgttc
    13681 agttttatcg agtaatgctt tgtttttctt cagaaatgac cagcgatgca acggcttttt
    13741 aaaacgccag cccagccaga aggagatcca gtcgcccagc agacaaccga caatccctgc
    13801 cagccaagcg tgccagaaac ttaactcgcc gctgccaatc agcgctccca gccccgccat
    13861 cagcaccgta ccgggtagaa tcaaaccgac cagcgccagc gactccagaa aggccaccaa
    13921 caccaccgcc atcaatgaat acacggtaga ttgggtaata aagtgttcca gcaatgcttg
    13981 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
    14041 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
    14101 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
    14161 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
    14221 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
    14281 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
    14341 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
    14401 aagcctcgcg tacacgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
    14461 gccgcagtaa caattgctca agcagattca tcgccagcag ctccgaatag cgcccttccc
    14521 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atacggctgg tgcgcttcat
    14581 ccgggcgaaa gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
    14641 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacaac
    14701 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt
    14761 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
    14821 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
    14881 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
    14941 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca
    15001 gacattgccg tcactgcgtc ttttactggc tcttctcgct aacccaaccg gtaaccccgc
    15061 ttattaaaag cattctgtaa caaagcagga ccaaagccat gacaaaaacg cgtaacaaaa
    15121 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct
    15181 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
    15241 tctctactgt ttctccatac ccgttttttt ggatggagtg aaacgatggc gattgcaatt
    15301 ggcctcgatt ttggcagtga ttctgtgcga gctttggcgg tggactgcgc cagcggtgaa
    15361 gagatcgcca ccagcgtaga gtggtatccc cgttggcaaa aagggcaatt ttgtgatgcc
    15421 ccgaataacc agttccgtca tcatccgcgt gactacattg agtcaatgga agcggcactg
    15481 aaaaccgtgc ttgcagagct tagcgtcgaa cagcgcgcag ctgtggtcgg gattggcgtt
    15541 gacagtaccg gctcgacgcc cgcaccgatt gatgccgacg gtaacgtgct ggcgctgcgc
    15601 ccggagtttg ccgaaaaccc gaacgcgatg ttcgtattgt ggaaagacca cactgcggtt
    15661 gaagaagcgg aagagattac ccgtttgtgc cacgcgccgg gcaacgttga ctactcccgc
    15721 tatattggcg gtatttattc cagcgaatgg ttctgggcaa aaatcctgca tgtgactcgc
    15781 caggacagcg ccgtggcgca atctgccgca tcgtggattg agctgtgcga ctgggtgcca
    15841 gctctgcttt ccggtaccac ccgcccgcag gatattcgtc gcggacgttg cagcgccggg
    15901 cataaatctc tgtggcacga aagctggggc ggcttgccgc cagccagttt ctttgatgag
    15961 ctggacccga tcctcaatcg ccatttgcct tccccgctgt tcactgacac ctggactgcc
    16021 gatattccgg tgggcacctt atgcccggaa tgggcgcagc gtctcggcct gcctgaaagc
    16081 gtggtgattt ccggcggcgc gtttgactgc catatgggcg cagttggcgc aggcgcacag
    16141 cctaacgcac tggtaaaagt tatcggtact tccacctgcg acattctgat tgccgacaaa
    16201 cagagcgttg gcgagcgggc agttaaaggt atttgcggtc aggttgatgg cagcgtggtg
    16261 cctggattta tcggtctgga agcaggccaa tcggcgtttg gtgatatcta cgcctggttt
    16321 ggtcgcgtac tcggctggcc gctggaacag cttgccgccc agcatccgga actgaaagcg
    16381 caaatcaacg ccagccagaa acaactgctt ccggcgctga ccgaagcatg ggccaaaaat
    16441 ccgtctctgg atcacctgcc ggtggtgctc gactggttta acggtcgtcg cacgccaaac
    16501 gctaaccaac gcctgaaagg ggtgattacc gatcttaacc tcgctaccga cactccgctg
    16561 ctgttcggcg gtttgattgc tgccaccgcc tttggcgcac gcgcaatcat ggagtgcttt
    16621 accgatcagg ggatcgccgt caataacgtg atggcgctgg gcggcatcgc gcggaaaaac
    16681 caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc cgctgcaaat tgttgcctct
    16741 gaccagtgct gtgcgctcgg tgcggcgatt tttgctgccg tcgccgcgaa agtgcacgca
    16801 gacatcccat cagcccagca aaaaatggcc agtgcggtag agaaaaccct gcaaccgcgc
    16861 agcgaacagg cacaacgctt tgaacagctt tatcgccgct atcagcaatg ggcgatgagc
    16921 gccgaacaac actatcttcc aacttccgcc ccggcacagg ctgcccaggc cgttgcgact
    16981 ctataaggac acgataatgg cgatttttga taattatgaa gtgtggtttg tcattggcag
    17041 ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc caacatgccg agcacgttgt
    17101 taatgcgctg aatacggaag cgaaactgcc ctacaaactg gtgttgaaac cgctgggcac
    17161 cacgccggat gaaatcaccg ctatttgccg cgacgcgaat tacgacgatc gttgcgctgg
    17221 tctggtggtg tggctgcaca ccttctcccc ggccaaaatg tggatcaacg gcctgaccat
    17281 gctcaacaaa ccgttgctgc aattccacac ccagttcaac gcggcgctgc cgtgggacag
    17341 tatcgatatg gactttatga acctgaacca gactgcacat ggcggtcgcg agttcggctt
    17401 cattggcgcg cgtatgcgtc agcaacatgc cgtcgttacc ggtcactggc aggataaaca
    17461 agcccatgag cgtatcggct cctggatgcg tcaggcggtc tctaaacagg atacccgtca
    17521 tctgaaagtc tgccgatttg gcgataacat gcgtgaagtg gcggtcaccg atggcgataa
    17581 agttgccgca cagatcaagt tcggtttctc cgtcaatacc tgggcggttg gctatctggt
    17641 gcaggtggtg aactccatca gcgacggcga tgttaacgcg ctggtcgatg agtacgaaag
    17701 ctgctacacc atgacgcctg ccacacaaat ccacggcgaa aaacgacaga acgtgctgga
    17761 agcggcgcgt attgagctgg ggatgaagcg tttcctggaa caaggtggct tccacgcgtt
    17821 caccaccacc tttgaagatt tgcacggtct gaaacagctt ccaggtctgc ccgtacagcg
    17881 tctgatgcag cagggttacg gctttgcggg cgaaggcgac tggaaaaccg ccgccctgct
    17941 tcgcatcatg aaggtgatgt caaccggtct gcagggcggc acctccttta tggaggacta
    18001 cacctatcac ttcgagaaag gtaatgacct ggtgctcggc tcccatatgc tggaagtctg
    18061 cccgtcgatt gccgtagaag agaaaccgat cctcgacgtt cagcatctcg gtattggtgg
    18121 taaggacgat cctgcccgac tgatcttcaa tacccaaacc ggtccagcga ttgtcgccag
    18181 cctgattgat ctcggcgatc gttaccgtct gctggttaac tgtatcgaca cggtgaaaac
    18241 accgcactcc ctgccgaaac tgccggtggc gaatgcactg tggaaagcgc aaccggatct
    18301 gccaactgct tccgaagcgt ggatcctcgc tggtggcgcg caccataccg tcttcagcca
    18361 tgcgctgaac ctcaacgata tgcgccagtt cgccgagatg cacgacattg aaatcacggt
    18421 gattgataac gacacccgcc tgccagcgtt taaagacgca ctgcgctgga acgaagtgta
    18481 ttacggattt cgtcgctaag tagccacatc aggtatgtaa cgcctgatgc gacgctgacg
    18541 cgtcttatct ggcctacacg ctgcgatttt gtaggccgga taagcaaagc gcatccggca
    18601 ttcaacgcct gatgcgacgc tggcgcgtct tatcaggcct acgcgctgcg attttgtaga
    18661 ccggataagc gaagcgcatc cggcacgaag gagtcaacat gttaaaagat ctcaaacgcc
    18721 aggtattaga agccaacctg gcgctgccaa aacacaacct ggtcacgctc acatggggca
    18781 acgtcagcgc cgttgatcgc gagcgcggcg tctttgtgat caaaccttcc ggcgtcgatt
    18841 acagcgtcat gaccgctgac gatatggtcg tggttagcat cgaaaccggt gaagtggttg
    18901 aaggtacgaa aaagccctcc tccgacacgc caactcaccg gctgctctat caggcattcc
    18961 cctccattgg cggcattgtg catacgcact cgcgccacgc caccatctgg gcgcaggcgg
    19021 gtcagtcgat tccagcaacc ggcaccaccc acgccgacta tttctacggc accattccct
    19081 gcacccgcaa aatgaccgac gcagaaatca acggcgaata tgagtgggaa accggtaacg
    19141 tcatcgtaga aacctttgaa aaacagggta tcgatgcagc gcaaatgccc ggcgttctgg
    19201 tccattccca cggcccgttt gcatggggca aaaatgccga agatgcggtg cataacgcca
    19261 tcgtgctgga agaggtcgct tatatgggga tattctgccg tcagttagcg ccgcagttac
    19321 cggatatgca gcaaacgctg ctgcataaac actatctgcg taagcatggc gcgaaggcat
    19381 attacgggca gtaatgactg tataaaacca cagccaatca aacgaaacca ggctatactc
    19441 aagcctggtt ttttgatgga ttttcagcgt ggcgcaggca ggttttatct taacccgaca
    19501 ctggcgggac accccgcaag ggacagaagt ctccttctgg ctggcgacgg acaacgggcc
    19561 gttgcaggtt acgcttgcac cgcaagagtc cgtggcgttt attcacgccg atcaggttcc
    19621 ccgcgctcag catattttgc agggtgaaca aggctttcgt ctgacaccgc tggcgttaaa
    19681 ggattttcac cgccagccgg tgtatggcct ttactgtcgc gcccatcgcc aattgatgaa
    19741 ttacgaaaag cgtctgcgtg aaggtggcgt taccgtctac gaggccgatg tgcgcccgcc
    19801 agaacgctat ctgatggagc ggtttatcac ctcaccggtg tgggtcgagg gtgatatgca
    19861 caatggcact atcgttaatg cccgtctgaa accgcatccc gactatcgtc cgccgctcaa
    19921 gtgggtttct atagatattg aaaccacccg ccacggtgag ctgtactgca tcggcctgga
    19981 aggctgcggg cagcgcatcg tttatatgct ggggccggag aatggcgacg cctcctcgct
    20041 tgatttcgaa ctggaatacg tcgccagccg cccgctgctg ctggaaaaac tcaacgcctg
    20101 gtttgcaaac cacgatcccg atgtgatcat cggctggaac gtggtgcagt tcgatctgcg
    20161 aatgctgcaa aaacatgccg agcgttaccg tcttccgctg cgtcttgggc gcgataatag
    20221 cgagctggag tggcgcgagc acggctttaa aaacggcgtc ttttttgccc aggctaaagg
    20281 tcggctaatt atcgacggta tcgaggcgct gaaatccgcg ttctggaatt tctcttcatt
    20341 ctcgctggaa actgtcgctc aggagctatt aggcgaagga aaatctatcg ataacccgtg
    20401 ggatcgaatg gacgaaattg accgccgttt cgccgaagat aaacctgcgc tggcaactta
    20461 taacctgaaa gattgcgagc tggtgacgca gatcttccac aaaactgaaa tcatgccatt
    20521 tttactcgaa cgagcaacgg tgaacggcct gccggtggat cggcacggcg gttcggtggc
    20581 agcgtttggt catctctatt ttccgcgaat gcatcgcgct ggttatgtcg cgcctaatct
    20641 cggcgaagtg ccgccgcacg ccagccctgg cggctacgtg atggattcac ggccagggct
    20701 ttatgattca gtgctggtgc tggactataa aagcctgtac ccgtcgatca tccgcacctt
    20761 tctgattgat cccgtcgggc tggttgaagg catggcgcaa cctgatccag agcacagtac
    20821 cgaaggtttt ctcgatgcct ggttctcacg ggagaaacat tgcctgccgg agattgtgac
    20881 taacatctgg cacgggcgcg ataaagccaa acgtcagggt aataagccgc tttcgcaggc
    20941 gctgaagatc attatgaatg ccttttatgg cgtgctcggc accaccgcct gccgcttctt
    21001 cgatccgcgc ctggcatcgt cgatcaccat gcgtggtcat cagatcatgc ggcagaccaa
    21061 agcattgatt gaagcgcagg gctacgacat tatctacgga gataccgact cgacttttgt
    21121 ctggctaaaa ggtgcacatt cggaagaaga ggcggcgaaa atcggtcgtg cactggtgca
    21181 gcacgttaac gcctggtggg cggaaacgct gcaaaaacaa cggctgacca gcgcattaga
    21241 actggagtat gaaactcatt tctgccgttt tcttatgcca accattcgcg gagctgatac
    21301 cggcagtaaa aagcgctacg ccgggctgat tcaggagggc gacaagcagc ggatggtgtt
    21361 taaagggctg gaaaccgtgc gcaccgactg gacgccgctg gcccagcagt ttcagcagga
    21421 gctgtacctg cgtatcttcc gcaacgagcc atatcaggag tacgtccgcc aaaccatcga
    21481 caaactgatg gcgggtgaac tggatgcgcg gctggtttac cgtaaacgcc ttcgccgtcc
    21541 gctgagcgaa tatcagcgta atgttccgcc tcatgtacgc gccgctcgcc ttgccgatga
    21601 agaaaatcaa aagcgcggtc gccccttgca atatcagaat cgcggcacca ttaagtacgt
    21661 atggaccacc aacggcccgg agccgctgga ctaccaacgt tcaccactgg attacgaaca
    21721 ctatctgacc cgccagctac aacccgtggc ggagggaata ctccctttta ttgaggataa
    21781 ttttgctaca cttatgaccg ggcaacttgg gctattttga gcaaaaaaaa gagttcgcca
    21841 gataccattt tgatgcgtga cgaatgcttt gccatccagt accatagcgc cctttccatt
    21901 cctggacctg aataacacca ctacctcata agcacggtag cgggtggtta ttgcctgcaa
    21961 ttaaagatat agagccgaac acatatgcct tttacacttg gtcaacgctg gatcagcgat
    22021 acagaaagcg aattgggact tggaaccgtt gtcgcggtgg atgcgcgaac tgtcacttta
    22081 cttttcccat ctactggtga aaaccgtctg tacgcacgca gtgattcccc cgtgacccgc
    22141 gtgatgttca accctggtga taccattacc agccatgacg gctggcagat gcaagtcgaa
    22201 gaagtaaaag aagaaaatgg cttgctgacc tatatcggta ctcgcctgga tactgaagag
    22261 tccggcgtag ccctgcgtga agttttcctt gatagcaaac tggtgttcag caaaccgcag
    22321 gaccgtctgt ttgccgggca gattgaccgt atggaccgct ttgcgctgcg ttatcgcgcg
    22381 cgtaagtatt ccagcgaaca gttccgtatg ccgtacagcg gcctgcgcgg tcagcgtacc
    22441 agcctgatcc cgcatcagct caacatcgct catgatgttg gtcgccgcca cgcgccgcgc
    22501 gtcctgctgg ctgacgaagt gggtttaggg aaaaccattg aagccgggat gatcctgcat
    22561 cagcaactgc tctctggcgc tgctgaacgt gtgctgatta tcgtcccgga aaccttacag
    22621 catcagtggc tggtagaaat gctgcgccgt ttcaacctgc gctttgctct gtttgatgat
    22681 gagcgttatg ccgaagctca gcacgatgct tacaacccgt ttgacaccga acagctggtg
    22741 atttgctcgc tggattttgc ccgtcgtagc aaacagcgct tggaacatct ctgtgaagcc
    22801 gaatgggacc tgctggtggt cgatgaagcg catcacctgg tgtggagcga agatgcgcca
    22861 agccgtgaat atcaggccat tgaacaactg gcagagcacg tgccgggcgt tctgctgctg
    22921 accgcgaccc cggaacagct ggggatggaa agccacttcg cccgtctgcg tctgctggac
    22981 ccgaaccgtt tccacgattt tgcgcagttc gttgaagagc agaaaaatta tcgtccggtt
    23041 gcggacgccg ttgccatgct gctggcaggt aacaaactga gcaatgacga actgaacatg
    23101 ctcggcgaga tgatcggcga gcaggatatc gagccgctgt tgcaggcagc aaacagcgac
    23161 agcgaagatg cccagagcgc ccgtcaggag ctggtttcga tgctgatgga tcgccacggc
    23221 accagccgcg tgctgttccg taacacgcgt aacggtgtga aaggattccc gaaacgcgag
    23281 ctgcacacca ttaagctgcc gctaccgacg cagtatcaga cggctattaa agtctccggc
    23341 attatgggcg cacgtaaaag tgcggaagat cgtgctcgcg atatgctcta cccggagcgt
    23401 atttatcagg aatttgaagg tgataacgcc acctggtgga acttcgatcc gcgcgttgag
    23461 tggctgatgg gctacctgac cagccatcgc tctcagaaag tgctggtgat ctgcgccaaa
    23521 gctgccactg cgctgcaact ggagcaggta ctgcgcgaac gtgaaggtat tcgcgctgcg
    23581 gtgttccacg aaggtatgtc gattatcgaa cgtgaccgcg ctgccgcctg gtttgccgaa
    23641 gaagacaccg gcgcacaggt actgctgtgc tcagaaatcg gttctgaagg acgtaacttt
    23701 cagttcgcca gtcacatggt gatgtttgac ctgccattca acccggatct actggagcag
    23761 cgtattggtc gtctggatcg tatcggccag gcgcacgata ttcagatcca tgtgccttat
    23821 ctggagaaaa ccgctcagtc ggtgctggtg cgctggtatc acgaaggtct ggatgcattt
    23881 gagcacacct gcccgaccgg acgcactatt tacgatagcg tatacaacga tctgattaac
    23941 tatctggctt caccggatca aaccgaaggc tttgacgatc tgatcaaaag ctgccgcgag
    24001 caacatgaag cgctgaaagc acagctggaa cagggtcgtg accgcctgct ggaaatccac
    24061 tccaacggtg gcgaaaaagc ccaggcactg gcagaaagca ttgaagagca ggatgacgat
    24121 accaacctga tcgccttcgc catgaacctg ttcgatatta tcggtatcaa tcaggacgat
    24181 cgcggcgaca acatgatcgt gctgacgccg tccgatcata tgctggtgcc ggacttccct
    24241 ggcctgtcgg aagatggcat caccatcacc tttgatcgtg aagtggcgct ggcgcgtgaa
    24301 gatgcacagt ttattacctg ggagcatccg ctgatccgca acggtctgga tctgatcctt
    24361 tctggcgata ccggtagcag cacgatttca ctgttaaaaa acaaagcgtt gccggtaggt
    24421 acgctgttgg tggaactgat ttatgtggtt gaagcccagg ctccgaagca gttgcagctc
    24481 aaccgcttcc tgccaccgac gccggtacgt atgctgctgg ataaaaacgg caacaacctg
    24541 gcggcgcagg tagagtttga aacctttaac cgccagctta acgcggttaa ccgtcacacc
    24601 ggcagcaaac tggttaacgc cgtgcagcag gatgttcacg ctatccttca actgggtgaa
    24661 gcgcagatcg agaaatctgc ccgtgcattg attgatgcag cgcgtaacga agccgacgaa
    24721 aaactgtctg ccgagctgtc tcgtctggaa gctctgcgtg cagtgaaccc gaacattcgt
    24781 gacgacgaac tgaccgccat tgagagcaac cgtcagcagg taatggaaag cctggatcag
    24841 gcaggttggc gtctggatgc cctgcgtttg atcgttgtaa cgcatcagta acggagccga
    24901 aaatggggat ggaaaactac aatccaccgc aggaaccctg gttggttatc ctgtatcagg
    24961 atgaccatat tatggtggtc aacaagccga gcggtttgtt gtcagtgccg ggtcgtctgg
    25021 aagagcacaa agacagcgtg atgacgcgca ttcagcgtga ttatccgcag gcagaatcgg
    25081 tgcatcgtct ggatatggct accagcggcg tgattgtagt ggcgctgacc aaagccgcgg
    25141 agcgggagtt aaaacgccag ttccgcgagc gcgagccgaa aaagcagtat gtggcccgcg
    25201 tctgggggca tccatccccc gcagaaggtc tggtggatct gccgctgatt tgcgactggc
    25261 caaaccgccc gaaacagaaa gtctgttacg aaacgggtaa acctgcgcag acggaatatg
    25321 aagtggtgga gtatgcggcg gataacacgg caagagtggt gttaaaaccg attaccgggc
    25381 gttcgcatca attgcgtgtg catatgctgg cgctggggca tccgattctc ggcgatcgtt
    25441 tttatgcatc accagaagcg agagcgatgg caccgcgttt gttgctacat gcagagatgt
    25501 tgacgattac ccatccggcg tatggcaata gtatgacgtt taaagcgcca gcggattttt
    25561 aatcgtcacg ctggcgcagt tcggtaggcg gataaggcgt tcacgccgca tccggcaatt
    25621 gtgcgccgat tgcctgatgc gacgcgggcc gcgtcttatc aggcctacag tttaaaactt
    25681 atttaaaccc tttctgctgc tttatcagct catatgcctg ctgaatttcc tgcgctttct
    25741 gcttcgccat ctccatcatc tcaggcggca aacctttcgc caccagctta tcgggatggt
    25801 gttcactcat cagcttacgg taggcacgtt tgatggtggt cgcatcatcc gtcggcttca
    25861 cgcccagcac attacaggca tcttccagcg tcgggccacg ctgcgcttgc tgccagttac
    25921 caccgccaga ttgctgctga taaccgccgc caaactgtgc accgccctgc atcatgcgca
    25981 aaaactggtc aaactgggcg cgggagatcc ctaattcttc agcaatgaca tacagcaccg
    26041 cccgttcatt cgggtgcagt gaaccatcag caaacgccgc ctgaatctgg atctccagaa
    26101 acatacgaat taagtcaaaa cgaccaaagc agacactgcg aaactggcgc atcttttcgc
    26161 gcagcgggta attatctgat tttcccaccc ggaacgcgtt ttgcgccgca gtacgggaag
    26221 cgccatgaag gttcattcgg tccatcaact ggctggcgat atgaatatca gcctccgtga
    26281 cgcgaccttt ggatttggtt aaatgcccca tcacttcaaa agtggtggca aaaaacagcg
    26341 cctgacgctc acgctggttg gcgaaccacg ccattttacg gctacgggct ttatcaaaca
    26401 tatggccaat taacaggcct aacactacgc cccaaaagcc gccgcccatc agtaaggcca
    26461 cggccacgcc aatgattttt ccccaatact gcatatattc cccaaatcga cacacggata
    26521 tcagggctat ctcccacaat ataaaggtgc ttttaccgtt ttccggctgc ggtcaattgt
    26581 gggacatcgc ctataatttg cattatcata cctgtcattc aatgccgtgc ctaacaccac
    26641 tgacgctatt cggacaggat taacactagc gtagagatga cgagtacgtt agtctctgac
    26701 cgtttgtcac gcgcaacgtt accgatgatg gaacaataaa atcaacgtat gaaaaaacgt
    26761 atccccactc tcctggccac catgattgcc accgcccttt atagtcaaca gggactggca
    26821 gccgacctcg cctcacagtg catgttgggc gtgccaagct atgaccgtcc tctggtacag
    26881 ggcgatacca atgacttacc cgtgactatc aatgctgacc acgcgaaagg ggactacccg
    26941 gatgacgccg tgtttactgg cagcgtggat atcatgcagg gtaacagccg tctgcaggcc
    27001 gacgaagtgc agctccatca aaaagaggca ccaggacaac cggagccggt acgtaccgtt
    27061 gatgcgctcg gtaatgtcca ttacgacgat aaccaggtga tcctcaaagg gccgaaaggc
    27121 tgggcgaatc tgaacaccaa agataccaac gtctgggaag gtgattacca gatggtgggt
    27181 cgccagggtc gcggtaaagc ggacctgatg aaacaacgtg gcgaaaaccg ctataccatt
    27241 ctggataacg gtagctttac ctcctgtctg ccgggttctg acacctggag cgtggtaggt
    27301 agcgaaatta ttcatgaccg cgaagaacaa gttgctgaga tctggaacgc ccgctttaag
    27361 gtgggtccgg taccgatctt ttatagcccc tatttgcagt tgccggtggg tgacaaacgt
    27421 cgctctggtt tcttgatccc gaacgccaag tacaccacca ccaactactt tgagttctac
    27481 ctgccatatt actggaacat cgcgccaaat atggatgcca ccatcacgcc gcattatatg
    27541 catcgtcgtg gcaacatcat gtgggagaac gaattccgct acctctccca ggcgggcgct
    27601 ggcttgatgg aactggacta tctgccttca gataaagtct atgaagatga acacccgaac
    27661 gatgacagtt cacgtcgttg gttattctac tggaaccact ccggggtcat ggatcaggtg
    27721 tggcgtttca acgtcgacta caccaaggtc agcgatccta gctacttcaa tgatttcgat
    27781 aacaagtacg gttccagtac tgacggctac gcaacgcaaa aattcagcgt tggctatgcg
    27841 gtgcaaaact tcaatgccac cgtttcaacc aagcagttcc aggttttcag cgaacagaac
    27901 accagtagct actcggcaga gccgcagtta gacgttaatt actaccagaa tgatgttggt
    27961 ccgtttgata cgcgtattta cggccaggca gtgcactttg ttaacaccag agacgacatg
    28021 cctgaagcaa cccgtgttca cctggaaccg accatcaatt tgccgctctc taataactgg
    28081 ggcagcatca ataccgaagc gaagtttctg gcaacccatt atcagcaaac caatcttgac
    28141 tggtataact ccagaaacac gaccaagctg gacgaatccg ttaaccgcgt aatgccgcaa
    28201 ttcaaagttg acggcaaaat ggtctttgaa cgcgatatgg aaatgctggc tccgggttat
    28261 acccaaacgc tggaaccgcg cgcgcagtat ttgtacgtgc cgtatcgcga tcagagcgac
    28321 atctataact acgactcgtc tctgctgcaa tctgactact ctggcctgtt ccgggaccgg
    28381 acttacggcg gtcttgaccg tattgcctcc gctaaccaag tgacgaccgg tgtcacatct
    28441 cgcatatatg atgatgctgc cgttgaacgt tttaatattt ccgttggtca aatctactat
    28501 ttcacggagt ctcgcactgg cgatgacaac ataacatggg agaatgacga caaaacgggc
    28561 tcactggtgt gggcaggcga cacctactgg cgtatctccg agcgttgggg attgcgtggc
    28621 gggattcagt acgatacacg tctggataac gtagcgacca gtaactccag cattgaatac
    28681 cgtcgggatg aagaccgtct ggtacagctg aattaccatt acgccagccc ggaatatatt
    28741 caggctacgc tgcctaagta ctattccact gctgagcaat ataagaatgg tatttcgcag
    28801 gtaggtgctg tcgccagcag gccaattgcc gatcgttggt ccattgttgg ggcctactac
    28861 tacgacacca atgcgaacaa gcaagccgac tctatgttag gtgtgcaata cagctcctgc
    28921 tgctatgcaa ttcgcgtcgg ttacgagcgg aagctgaacg gttgggataa cgataaacaa
    28981 catgcggtat atgacaacgc aatcggcttt aacatcgaac ttcgcggcct gagctccaac
    29041 tacggtctgg gtacgcaaga gatgctgcgt tcgaacattc tgccgtatca aaacactttg
    29101 tgatttgttg atttaccacg taatccgcag tgcggttaat tgaaatggaa aaagtatgaa
    29161 gaactggaaa acgctgcttc tcggtatcgc catgatcgcg aataccagtt tcgctgcccc
    29221 ccaggtagtc gataaagtcg cagccgtcgt caataacggc gtcgtgctgg aaagcgacgt
    29281 tgatggatta atgcagtcgg taaaactgaa cgctgcgcag gcaaggcagc agcttcctga
    29341 tgacgcgacg ctgcgccacc aaatcatgga acgtttgatc atggatcaaa tcatcctgca
    29401 gatggggcag aaaatgggag tgaaaatctc cgatgagcag ctggatcagg cgattgctaa
    29461 catcgcgaaa cagaacaaca tgacgctgga tcagatgcgc agccgtctgg cttacgatgg
    29521 tctgaactac aacacttatc gtaaccagat ccgcaaagag atgattatct ctgaagtgcg
    29581 taacaacgag gtgcgtcgtc gcatcaccat cctgccacag gaagtcgaat ccctggcgca
    29641 gcaggtgggt aaccaaaacg acgccagcac tgagctgaac ctgagccaca tcctgatccc
    29701 gctgccggaa aacccgacct ctgatcaggt gaacgaagcg gaaagccagg cgcgcgccat
    29761 tgtcgatcag gcgcgtaacg gcgctgattt cggtaagctg gcgattgctc attctgccga
    29821 ccagcaggcg ctgaacggcg gccagatggg gtggggccgt attcaggagt tgccggggat
    29881 cttcgcccag gcattaagca ccgcgaagaa aggcgacatt gttggcccga ttcgttccgg
    29941 cgttggcttc catattctga aagttaacga cctgcgcggc gaaagcaaaa atatctcggt
    30001 gaccgaagtt catgctcgcc atattctgct gaaaccgtcg ccgatcatga ctgacgaaca
    30061 ggcccgcgtg aaactggagc aaattgctgc tgatatcaag agtggtaaaa cgacttttgc
    30121 tgccgcagcg aaagagttct cgcaggatcc aggttctgct aaccagggcg gtgatctcgg
    30181 ctgggctaca ccagatattt tcgatccagc cttccgtgac gccctgaccc gcctgaacaa
    30241 aggtcaaatg agtgcaccgg ttcactcttc attcggctgg catttaatcg aactgctgga
    30301 tacccgtaat gtcgataaaa ccgacgctgc gcagaaagat cgtgcatacc gcatgctgat
    30361 gaaccgtaag ttctcggaag aagcagcaag ctggatgcag gaacaacgtg ccagcgccta
    30421 cgttaaaatc ctgagcaact aatggttaaa acccaacgtg ttgtgatcac tcccggcgag
    30481 cccgccggga ttggcccgga cttagttgtc cagcttgcac agcgtgagtg gccggtcgaa
    30541 ctggttgttt gtgccgatgc cactctcctt accgaccggg cagcgatgct cggtttgccg
    30601 ctcaccctcc gcccttattc ccccaactcc cctgcacaac cgcaaactac gggcacatta
    30661 acgctacttc ctgtcgcgct acgtgaatct gtcactgcgg ggcagttagc gattgaaaat
    30721 gggcattacg tggtggagac gctggcgcga gcgtgtgatg gctgtctgaa cggtgaattt
    30781 gctgcgctga tcacaggccc cgtgcataaa ggcgtcatta acgacgcaag cattccgttt
    30841 accggtcata ccgagttttt cgaagagcgt tcgcaggcga aaaaagtggt gatgatgctg
    30901 gcgaccgaag aacttcgcgt ggcgctggca acgacgcatt taccgctgcg cgatatcgca
    30961 gatgctatca cccctgcact tttgcacgaa gtgattgcta ttttgcatca cgatttgcgt
    31021 acaaaatttg gtattgccga accgcgcatt ctggtctgcg ggctgaatcc gcacgcgggc
    31081 gaaggcggtc atatgggtac ggaagagata gacaccatta ttccggtgct cgacgagctg
    31141 cgggcgcagg ggatgaaact caacgggccg ctgcctgccg ataccctgtt tcagccgaaa
    31201 tatctcgata acgccgacgc cgtgctggcg atgtaccacg atcagggtct tcccgtgcta
    31261 aaataccagg gcttcgggcg cggtgtgaac attacgctgg gcctgccctt tattcgcaca
    31321 tcagtggacc acggcaccgc gcttgaactg gcgggacgtg gcgaagccga tgtcggcagt
    31381 tttattacgg cgcttaatct cgccatcaaa atgattgtta acacccaatg aataatcgag
    31441 tccaccaggg ccacttagcc cgtaaacgct tcgggcaaaa ctttctcaac gatcagttcg
    31501 tgatcgacag cattgtttct gccattaacc cgcaaaaggg ccaggcaatg gtcgaaatcg
    31561 gccccggtct ggcggcattg accgagccgg tcggcgaacg tctggaccag ctgacggtca
    31621 tcgaacttga ccgcgatctg gcggcacgtc tgcaaacgca tccattctta ggcccgaaac
    31681 tgacgattta tcagcaggat gcgatgacct ttaactttgg cgaactggcc gagaaaatgg
    31741 gtcagccgct gcgtgttttc ggcaacctgc cttataacat ctccacgccg ttgatgttcc
    31801 atctgtttag ctatactgat gccattgccg acatgcactt tatgttgcaa aaagaggtgg
    31861 tgaatcgtct ggttgcagga ccgaacagca aagcgtatgg tcgattaagc gtcatggcgc
    31921 aatactattg caacgtgatc ccggtactgg aagttccgcc gtcagccttt acaccaccac
    31981 ccaaagtgga ttccgccgtc gtgcgcctgg tccctcatgc aacgatgcct cacccggtta
    32041 aagatattcg cgtgttgagc cgcatcacca ccgaagcctt taaccagcgt cgtaaaacca
    32101 ttcgtaacag cctcggcaac ctgtttagcg tcgaggtgtt aaccggaatg gggatcgacc
    32161 cggcgatgcg agcggaaaat atctctgtcg cgcaatattg ccagatggcg aactatctgg
    32221 cggagaacgc gcctttgcag gagagttaac gatgatcaat tcgccccgag tgtgtattca
    32281 ggttcaaagc gtctacattg aggcacaatc ttcacctgat aatgaacgtt acgtttttgc
    32341 ttataccgta accatacgca atctggggcg agcgccagtg cagttgttgg ggcgttactg
    32401 gctgatcacc aatggcaatg gccgtgaaac cgaagtccag ggcgaaggag tggttggcgt
    32461 ccagccactt atcgcgcctg gcgaagagta ccagtacacc agcggtgcaa tcattgaaac
    32521 cccgctgggc accatgcagg gtcactacga aatgatcgat gaaaatggcg tccctttcag
    32581 catcgacatt cccgtattcc gactcgccgt tcccacactc attcattaaa agaatatggc
    32641 gacatacctt attggcgacg ttcatggttg ttacgatgaa ctgatcgcat tgctgcataa
    32701 agtagaattt acccccggga aagataccct ctggctgacg ggcgatctgg tcgcgcgcgg
    32761 cccgggttcg ctggatgttc tgcgctatgt gaaatcctta ggcgacagcg tacgtctggt
    32821 gctgggcaat cacgatctgc atctgctggc ggtatttgcc gggatcagcc gcaataaacc
    32881 gaaagatcgc ctgacaccgc tgctggaagc gccggatgcc gacgagctgc ttaactggct
    32941 gcgtcgccag cctctgctgc aaatcgacga agagaaaaag ctggtgatgg cccacgcagg
    33001 gatcacgccg cagtgggatc tgcagaccgc caaagagtgc gcgcgcgatg tagaagcggt
    33061 gctgtcgagt gactcctatc ccttctttct tgacgctatg tacggcgata tgccaaataa
    33121 ctggtcaccg gaattgcggg ggctgggaag actgcgtttt atcaccaacg cctttacccg
    33181 tatgcgtttt tgcttcccga acggtcaact ggatatgtac agcaaagaat cgccggaaga
    33241 agcccctgcc ccactgaaac cgtggtttgc gattcctggc cctgtcgctg aagaatacag
    33301 catcgccttt ggtcactggg catcgctgga aggcaaaggt acgccggaag gtatttacgc
    33361 gctggatacc ggctgctgct ggggtgggtc attaacctgc ctgcgctggg aagataaaca
    33421 gtattttgtc cagccgtcga accggcataa ggatttgggc gaagcggcgg cgtcttaaac
    33481 acagcctgat acaggaaggc cggataagac gcgaccggcg tcgcatccgg cactagccgt
    33541 aaattctata caaacttacc gccgctccag aatctcaaag caatagctgt gagagttctg
    33601 cgcatcggcg tcgtggaatt cgctgaatac cgattcccag tcatccggct cgtaatccgg
    33661 gaaatgggtg tcgccttcca cttctgcgtc gatatgcgtc agatacagtt tttgcgcttt
    33721 tggcaggaac tgctcataaa cgcgaccgcc gccaatcacc atgatttctg gtacgtcacc
    33781 acacgccgca atggcttcat ccaccgattt cacccacgtt acgcgatcgt ccgtacccgg
    33841 ttgactgctg aggataatat ttttgcgtcc tggcaacgga cgaccgattg attcccaagt
    33901 atggcggccc ataatcacgg gtttatttaa ggtgttgcgt ttaaaccagg cgagatcggc
    33961 aggcaggttc cacggcatgg cgttttccat gccgataacg cgatctaccg ctaacgccgc
    34021 aatcagactg atcattgaga tttcccgata aaaaaattgt cgccactata cgtaaagcgt
    34081 aaaccgtcgt cgactggtgc gaggatgatg ttgaggaaaa ttttatattc tgctggcgag
    34141 tccacgctct ccccctggac tcgccgcatt acaatgaaac aaaaacaaac agttagctgt
    34201 aaagtgtgat ttacgtcact ctttattaag atgagggttt cgtttccggt tcatccgcca
    34261 tgttgccggt atgtttacct tcttctgttc cctgccagcc atgtcgttga attaatgaca
    34321 gatgttcgcg gtcctcggtg atgatctcac ttaacatcgc gctggtgcgt ttatagaccg
    34381 ccgcgcgggc tttggtgtca ttctcaacca ttgccatctc ttccaccatc tgaatattaa
    34441 agcggcggaa cacatcggca cgttctcgcg cttcatacgg ccccagacct aaactttcca
    34501 gcgccagacg cccggttttc agcgcacctt caaaggtttc acgctccggc ttttcaacgc
    34561 ctgcctgacg caaacgaatg tagtgatcga catcgcgggc gcgggcaata atctgcaaat
    34621 gcgggaaatg ttctttcacc atctccgtca gttgcaggtt ggtttgcgga tcgtcaatag
    34681 cgttaatcag cacttccgct ttcgccgctc cggcagattc cagtaaatcc atccgcgtgg
    34741 catcgccata aaacactttc ataccaaatt tacgcagcgt ttcgatatgg tccggatcgt
    34801 gatcgagcac caccattttc accccgctgg agagcagtaa acgtccggta atctgtccaa
    34861 aacgaccgaa tccggcgata atcacgcgcg gctgttcttc atcgatctca tcggcttcac
    34921 gcgcctcctc cgtagaagat tgctcaaggc gattgaggat caccagcaga atcggcgttg
    34981 ctgccatcga cagcgccacc gccagggtca gcgatttcac ccactccggc tccagcacat
    35041 tcgccatctg cgccgcacca aataccacaa aggcaaactc actgccctgc cctaacaaca
    35101 ccgcaaacca gcgacgctgt ttatttggca cctgcaacgg tcgggcaatc agccacagca
    35161 tggcgatttt gatgatgagg aaaccgagca gcaaaatgac aatgcgcaat gggttttcaa
    35221 tcagcgtgcc aaagtctatc gacatgccaa caccgatgaa aaacagcccc aacagcaaac
    35281 ctttaaacgg ttcgatatcg ctttccaacg catgacggta ttccgagctt gccagcagta
    35341 cgcccgccag aaacgcgccc atcgccatcg acaagccgac ctcttccagc agcaaaccaa
    35401 agccaaacac aaggaataac gccacggcac taaacacttc ccgcaagcca gagcgggcaa
    35461 caaaacgcag cgccggacgc gtaacatagc gccccagcaa taccaccagt accagcgcac
    35521 ccgccacttt taacgccgag agagcaaatg cgcccatcgt cgtcgaggca ctgctcgccg
    35581 ccagtagcgg gatcatcgcc accagcggga ttgccgcgat atcctggaac agcagcactg
    35641 caaaggcact gcggcccatt tgcgtcacca tcagattgcg ttcattcatc gcctgcatgg
    35701 caatcgccgt tgatgagagc gccagcgtca taccgatcaa ttccgcgacc tgccagcgca
    35761 gcccaagtaa catacagaac agccccagca ggccaccgca aatcaccatc tgcaatgcgc
    35821 caccgccgaa cactgccgca cgcagcttcc acagcctttg tggatcgagt tcgaggccga
    35881 taataaacag catcagcacc accccaatct cggcaaagtg cagaatagat tcggcatcgg
    35941 tcaccagtcg cagcccccac gggccaataa tgcagccggc gatcaggtag ccaagtaccg
    36001 atcccagacc aagacgtacc gcaatgggta caatcagcgc cgccgatccg aggtagatca
    36061 gcgcctgaac cagcgtatga ctatccatga tgggcctcct gccattccag cagacgttgc
    36121 ttatagtggc gcgcctgccc ttcgagggtt tcgtcgtcac aaataaaggt gcagtgcatg
    36181 gcaaacggtg gcagccagtt cagcccgcag tagattgccg tcgcctgtag cggctgcgac
    36241 agcacatcaa agcccggatg cgcaccaatt tcaaaatggc tttccccgcc gccggtcgtc
    36301 accgcccaca gcaaatgttt gccatgcagc gccgtgccgc cgtgaccgta agcccagccg
    36361 tgcgacaaaa ctttatcgat ccaaagttta agaagcggag gaatgctgta ccactgcatc
    36421 gggtgctgcc agacgatcag atcggcgcga gagagcgcct cctgctcggc ggcaatatcg
    36481 atattgaagt caggatagag ttgataaaga gagcgaattt cgacgccttc cagcgtcctt
    36541 gcctgttcaa gcatccgttt attcgcatgg gaatgatgcg gatacggatg cgcataaatg
    36601 atgaggatca tagttattag cccgcgtgtt tacgcctttg ttttcccaca aagtgtagtc
    36661 atatattcac gcggcttata gtcaataata ttgattcaga aaagagagaa atctgaacaa
    36721 ataatcccat tctctgcccg cggatcgtca tattgctggt ctgcgccagt gacattcctc
    36781 gagtctccgg ggcaaacgct acggaaatca acaagccaaa cagcgagata cccgccccca
    36841 ttagcatcgt gttactgata ccgtaattat tgataaatat cggcagtgcc caggtcgaaa
    36901 caatggtgcc aatacgactt aaggacataa tcacgcccac ggcagaggcg cggatatccg
    36961 tcgggaagag ttcattagga tagagccact gcaaattacc cggcccgcca gagaaaaagg
    37021 catacaccgc aaaggccatc actaccagcc agatccccat atccgggatt agccccaaaa
    37081 ccgccaacgc cagcgtcatc atggcaaagc tgccaatcaa caatggacgc cgtccggcag
    37141 tgtttagcca cagcatcggc ggaatacagc cgagcataaa gaacaggcta atcaccacat
    37201 tccctagtgc cgcgtttttg ccaaccccca atcccaacga accaacgatt tgcgggccaa
    37261 aggtgtaaat ggcgaacatt gggatcacct ggcaggtcca gatggcggca acaaacagaa
    37321 caaaaggaaa atggcggcga ttaaacagat cgcgaaaacg ggtttgctgc ggctgctctt
    37381 catcgaaagc caccggttcg ccaaacagtt ttatcatcat ctcttcgcac tcttttactc
    37441 gccctttgcg taataaccag cggggagatt ctggcagttc gaatcgacca atcaaaatca
    37501 acaaacaagg gatcgccgcg ctacccagca tccagcgcca accaccttcc acatcataaa
    37561 gccagtagcc gaccagatcg gcgcaggtcg cgccgacata ccacatcgcg gcaataaagc
    37621 tgatggaaaa cgcccgctga cgggtactgg agaactcggt gatcatcgaa gtggcgatgg
    37681 gataatctgc accgatgaca atgccgatga gtacccgcat caccaacagt tcgacggggg
    37741 atgaaacaca catcgtcgcc accgatatca cgccgatggc gatgatatca atgaggaaca
    37801 ttttgcgccg tccgacttta tcggagatat agccaaacag cgatgtgcca acgaacagcc
    37861 cggcgagcgt tcccgcgccc agcaagccaa tccagtcagc gtccagtttc agcgccggag
    37921 tcagttgctc cagcgccacg ccaatcatta ccagtacata accatccaga aacggaccac
    37981 cgcttcccca cagcaaaatg cggcggtgaa tagaggagaa tttgagatcg tcaaagtttc
    38041 tggacggttg catgggttat tcctgttttt ttgcaatgag agtcagtacg gcgttacgta
    38101 tcaaaccaac atcagccgta acggaactcc acaccaaagg tgccgcgcgg gtattcccac
    38161 tgttccagcg ccgaccccag ccccagaatg cgacaggtgc cgcactccag acatccggcg
    38221 taatcgaagc gcacactgcc gtcatcctgc ttcttgtaca gacctgcggg gcacgctttc
    38281 accagcagct ccagcacctg tttatcagca tcggccttca caacaatgtg cggatgctct
    38341 tcatcgacat tgaatttatt gacgcccagt ttgacgtcca cattgacggg agaagtcata
    38401 aaacggtcac tcctttcatg ccatccttga tcagattgat gaagcccact ttcttgccgt
    38461 ggcggaggat tttcttgcgc atcagttccg gcgcactgcc gtcaatggtg aacaggtcac
    38521 gcgccacgcc caccgccagt tccgggttgc cgctaaacat gcgtgggtta tcaaggaacg
    38581 ccggtagttt ctggtacatg cgcatatcgc gcagcgggcc actctccaga tgctgacgat
    38641 attccgcgag tttttgctta ctgaaatcgt cgcttttcat cgctgaaagc acggtttttg
    38701 ctgcagcttc cccggcggca atcgccagat ccataccgcg aatggtaaaa ccgaggttca
    38761 tacacattcc ggcggcatca ccggcaatca atacgccgtc accgaccagc tccggcagca
    38821 tgttgatgcc tgcttccggc actacgtgag cggaatattc caccagcttg ccgcccgcga
    38881 tcagcggtgc aacggccgga tgctgtttga aatcttccag catttgcggc accgattttt
    38941 tcgcgtcatg cagatgatgc agaccacaaa ccagccccag cgacagggta ttttcattgg
    39001 tataaaggaa gccgccgccc atcaggccat cggtgggtga tcccgcaaac agacaagccg
    39061 cgccctgatt accctgcaac tgaaaacggt cttcgattac cgacttcggt aactcgatca
    39121 gttccttcac gccaaccgcc acatccgtcg gtttaacgcg ttttgccatc cccaactttt
    39181 cggcaaggat ggagttcacc ccatcagcaa ggagcaccgt tttcgcttca atcacatcac
    39241 catcggcttc tacaccgacg actttgccat cgcgctgtac gaggttatcg acgcggatcc
    39301 cggtaattaa ctgcgcgccc gcttcttcgg cctgctccat caaccaggca tcaaatttac
    39361 tgcgcaaaac ggagtaagaa cgctgggatg gcgaggtttc gtcaccgttg cagtagtcca
    39421 tagtcatcgc tgacttttcc gtcataaacg cgagtttttc atgggtgatc aggcgttcta
    39481 cgggggcgga atcggcgaaa cctggaataa tgtgttccag actgtgggca tagagacgcc
    39541 cgccagtaac gttcttggca cctgcggaat tgccacgctc gataactaac acttgcgccc
    39601 cttcgcgggc gagcaccagt gcggcaaccg aaccggcaag ccctgcaccg acgatgatgg
    39661 cgtcaaagat atcttcggac ataagagctc cctgtcagcg gcagagccgt aatgcagccc
    39721 tgccagagtg gatcaacgcg ctaaagctgc ggtcagtgcc ggaaggatct tcacggcatc
    39781 gccaacaatg ccgtaatccg cgtactggaa gatcggcgca tttttatctt tattgatggc
    39841 gaaaatggtt tgcgacgcgt tagcgccaac catgtgctgg atctacccgg agatccccac
    39901 cgccaggtac agttcaggtt tcagcatcag gttggagata ccgacatagc gttcgtgctc
    39961 catccatttt tcgttttctg ccaccggacg agaacaggcc aactccgcac ctatcgcctt
    40021 gcaaagctgt tctgccagcg caatgttctc tttgctgcca atgccgcgac cgacgctgac
    40081 caccagacgg gctttgtcga gatcgacgct gttgctctgg cgcgcctggg ttgccgtgcg
    40141 ggtaatcgcc acagccggag cctgccactc cacggtgtgc gtttcgccag tgcgtgacgc
    40201 gtctggctga gccgcatcga acgtgccgct gctgatggtc agtaccgcat acggcgtggc
    40261 aatgcgttct tcgccaatcg ccagaccgcc gtaaaccatg tgtttcactg ttgctttacc
    40321 gtcctgtacg ctgacggtgc tggcatcgtt agacaccgcc gctttaaggc gataacccag
    40381 ttttgccgcc agtaatttgc cgcgacgggt gtttggcaac agcaccagac cgtcagcgcc
    40441 gtgctggcga atagtgtcag ccatgacacc ggcgtaatct tcgatcatcc gatcgtccgg
    40501 tttgccgttt aatttccaga catgattagc gccgagctgg attgcctgtg cgccgtcggc
    40561 atcattgagg acaaaggcat tgatttgatt agctaaagcc tgcgcaccgt tcatcagttc
    40621 cggcagacga gagggggtat cgctgaatac ccagacttga gaaaacgtgt tcatagcatc
    40681 ccctgtaatt aaatgacttt gcgaagattt tcagcaaatg cggcgatctg ttcttcgccg
    40741 tcgccttcaa tcacgatgcg ctgacgctcg cgctgtttcg gcgcggcaac ctgttgttca
    40801 gaccaggcgt ccacggcgtt aaaaccaata tccgccgccg accatacctg gactggcttt
    40861 ttcgccgcgc cgagaatggc tttcatcgaa ggaatttgtg gggagttgat atcagtggaa
    40921 acagcaacaa ccgcaggcag cggaatgctt aaggtttcgg tttcatcttc cagttcgcgc
    40981 tcaacggtga gggtatctgc cgtcagggag ataattttgc tgacgccgtt aactgccgga
    41041 atattgagga tttcgcccac cagcagacca acctgctggg cataaaggtc ggaagaacca
    41101 tcgccacaga ggatcagatc aaagcctgct ttctgggcgg ctgcagccag tgcgctcgcc
    41161 gtttgttgcg gcagtgcctg ctcgaactgg tcatcaatca ccacaatcag ttcatccggg
    41221 ccgcgcgata gcacatcttt acgccctttg gcgttggtca gggctttacc gcccacactt
    41281 aaggctgtca cctgcgcctc tgctgcctgt tgctttagct ggcaagccgc ttcaatagcg
    41341 ttgagatcgt attggcttat tttggcatcg gctttgctga agtctaatga accatcagca
    41401 ttattgaccg caatatcctg ttcatcaggc acgcacttat agcaagtaat aatcttcatt
    41461 gcatctccag aaatcatgaa ggaaacgtcg ttcatatcgg tggtgttaat tacagatatc
    41521 acgcccttta atttggaaat aaaactttaa tcaccaatat tgaaagtcag atgctatttt
    41581 tttacccaca aaagaatata acaaactgat taataaccaa aattaacgaa aaacgccctc
    41641 tgttcactcg aaacaaaaat gtgataccaa tcaca
//
LOCUS       DAZOYF010000016        40544 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZOYF010000016 DAZOYF010000000
VERSION     DAZOYF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.016"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..956)
                     /locus_tag="ACHUOF_000848"
     CDS             complement(<1..956)
                     /locus_tag="ACHUOF_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFR0610141.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="ACHUOF_000849"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="ACHUOF_000849"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFR0610142.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHUOF_000850"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHUOF_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHUOF_000851"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHUOF_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR0610143.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="ACHUOF_000852"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="ACHUOF_000852"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR0610144.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="ACHUOF_000853"
     CDS             complement(5018..6346)
                     /locus_tag="ACHUOF_000853"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0610145.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8696)
                     /locus_tag="ACHUOF_000854"
                     /pseudo
     CDS             complement(6354..8696)
                     /locus_tag="ACHUOF_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="ACHUOF_000855"
     CDS             complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="ACHUOF_000855"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR0610146.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="ACHUOF_000856"
     CDS             complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="ACHUOF_000856"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR0610147.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="ACHUOF_000857"
     CDS             complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="ACHUOF_000857"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR0610148.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="ACHUOF_000858"
     CDS             complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="ACHUOF_000858"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR0610149.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="ACHUOF_000859"
     CDS             complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="ACHUOF_000859"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR0610150.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15311..16546
                     /gene="moeA"
                     /locus_tag="ACHUOF_000860"
     CDS             15311..16546
                     /gene="moeA"
                     /locus_tag="ACHUOF_000860"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR0610151.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16546..17295
                     /gene="moeB"
                     /locus_tag="ACHUOF_000861"
     CDS             16546..17295
                     /gene="moeB"
                     /locus_tag="ACHUOF_000861"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR0610152.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="ACHUOF_000862"
     CDS             complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="ACHUOF_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR0610153.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18267..19166
                     /locus_tag="ACHUOF_000863"
     CDS             18267..19166
                     /locus_tag="ACHUOF_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR0610154.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19172..21604
                     /locus_tag="ACHUOF_000864"
     CDS             19172..21604
                     /locus_tag="ACHUOF_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR0610155.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21750..22565
                     /gene="ybiV"
                     /locus_tag="ACHUOF_000865"
     CDS             21750..22565
                     /gene="ybiV"
                     /locus_tag="ACHUOF_000865"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR0610156.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22717..23982
                     /locus_tag="ACHUOF_000866"
     CDS             22717..23982
                     /locus_tag="ACHUOF_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR0610157.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24223..25815)
                     /locus_tag="ACHUOF_000867"
     CDS             complement(24223..25815)
                     /locus_tag="ACHUOF_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR0610158.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26034..26954
                     /gene="ldtB"
                     /locus_tag="ACHUOF_000868"
     CDS             26034..26954
                     /gene="ldtB"
                     /locus_tag="ACHUOF_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0610159.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27012..28130)
                     /locus_tag="ACHUOF_000869"
     CDS             complement(27012..28130)
                     /locus_tag="ACHUOF_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR0610160.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="ACHUOF_000870"
     CDS             complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="ACHUOF_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR0610161.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28780..28908
                     /gene="mntS"
                     /locus_tag="ACHUOF_000871"
     CDS             28780..28908
                     /gene="mntS"
                     /locus_tag="ACHUOF_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR0610162.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29180..30763
                     /gene="opgE"
                     /locus_tag="ACHUOF_000872"
     CDS             29180..30763
                     /gene="opgE"
                     /locus_tag="ACHUOF_000872"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR0610163.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="ACHUOF_000873"
     CDS             complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="ACHUOF_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR0610164.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31379..31444)
                     /locus_tag="ACHUOF_000874"
     CDS             complement(31379..31444)
                     /locus_tag="ACHUOF_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610165.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31679..32566
                     /gene="rhtA"
                     /locus_tag="ACHUOF_000875"
     CDS             31679..32566
                     /gene="rhtA"
                     /locus_tag="ACHUOF_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR0610166.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32865..33368
                     /gene="dps"
                     /locus_tag="ACHUOF_000876"
                     /gene_synonym="pexB"
     CDS             32865..33368
                     /gene="dps"
                     /locus_tag="ACHUOF_000876"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR0610167.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33772..34518
                     /gene="glnH"
                     /locus_tag="ACHUOF_000877"
     CDS             33772..34518
                     /gene="glnH"
                     /locus_tag="ACHUOF_000877"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR0610168.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34657..35316
                     /gene="glnP"
                     /locus_tag="ACHUOF_000878"
     CDS             34657..35316
                     /gene="glnP"
                     /locus_tag="ACHUOF_000878"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR0610169.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35313..36035
                     /gene="glnQ"
                     /locus_tag="ACHUOF_000879"
     CDS             35313..36035
                     /gene="glnQ"
                     /locus_tag="ACHUOF_000879"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR0610170.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36296..38520
                     /gene="ybiO"
                     /locus_tag="ACHUOF_000880"
                     /pseudo
     CDS             36296..38520
                     /gene="ybiO"
                     /locus_tag="ACHUOF_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="ACHUOF_000881"
     CDS             complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="ACHUOF_000881"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR0610171.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39719..39979
                     /gene="mcbA"
                     /locus_tag="ACHUOF_000882"
     CDS             39719..39979
                     /gene="mcbA"
                     /locus_tag="ACHUOF_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR0610172.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40244..>40544
                     /locus_tag="ACHUOF_000883"
     CDS             40244..>40544
                     /locus_tag="ACHUOF_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0610173.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT        10097 a        10719 c         9866 g         9862 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta
     8701 atacatttaa caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc
     8761 ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
     8821 gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct
     8881 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg
     8941 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
     9001 cgtgcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga
     9061 aatgacaggc tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg
     9121 atagaagaga cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca
     9181 atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
     9241 aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg
     9301 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca
     9361 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc
     9421 agcaacgtcc ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt
     9481 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag
     9541 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     9601 ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg
     9661 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     9721 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     9781 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     9841 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     9901 cgcgcatttc tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     9961 ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg
    10021 ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
    10081 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc
    10141 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
    10201 aactgtctgc tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa
    10261 acatccccag cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc
    10321 gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
    10381 ccgcccagac catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct
    10441 catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
    10501 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
    10561 gttctataac ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca
    10621 gcatgtggac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
    10681 gtaatcgttt gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc
    10741 gtcttcaaag ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg
    10801 tgccgacacc agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc
    10861 ctgcgccgcc ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc
    10921 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca
    10981 gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca
    11041 gccagtgtag aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc
    11101 ccgctgtccg gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg
    11161 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
    11221 actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
    11281 aggccacggt ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg
    11341 agttgcatag cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag
    11401 cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg
    11461 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc
    11521 ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
    11581 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
    11641 ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc
    11701 atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt
    11761 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg
    11821 tttgagggta atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata
    11881 caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac
    11941 cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt
    12001 gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
    12061 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
    12121 gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc
    12181 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
    12241 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
    12301 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
    12361 ctgccacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
    12421 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
    12481 aaggatgctg cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga
    12541 gatacatcac cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa
    12601 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
    12661 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
    12721 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
    12781 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
    12841 cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg
    12901 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
    12961 atgcctgaag tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
    13021 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
    13081 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
    13141 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa
    13201 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
    13261 cctgtttcgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
    13321 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
    13381 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
    13441 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
    13501 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
    13561 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
    13621 tcatcactcg ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt
    13681 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
    13741 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg
    13801 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc
    13861 gcatttgtgc agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca
    13921 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
    13981 cagtcactga cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca
    14041 gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
    14101 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg
    14161 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
    14221 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
    14281 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
    14341 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
    14401 aagacttcgc ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
    14461 gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg
    14521 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
    14581 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
    14641 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
    14701 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
    14761 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
    14821 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
    14881 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
    14941 gcttccgtca ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca
    15001 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
    15061 ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat
    15121 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
    15181 ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg
    15241 tggcatacag tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
    15301 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
    15361 gctttctcgc gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg
    15421 tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc
    15481 tatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
    15541 cggtaaatcc tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg
    15601 tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac
    15661 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
    15721 ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac
    15781 taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa
    15841 agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
    15901 cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg
    15961 ttgcgaggta atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt
    16021 tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga
    16081 agcggattac accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc
    16141 gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct
    16201 gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
    16261 aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc
    16321 ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc
    16381 cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt
    16441 tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga
    16501 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
    16561 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
    16621 caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca
    16681 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg
    16741 gtttcgctct cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg
    16801 aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca
    16861 gtcaatgcac tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg
    16921 ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg
    16981 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact
    17041 tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta
    17101 acctgcgtag aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca
    17161 atggaagcga tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg
    17221 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag
    17281 gtgtgcgggc agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg
    17341 catttgctcc agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca
    17401 gatttccaag cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat
    17461 gcagaacgtc cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact
    17521 tcgtcacagc ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca
    17581 gagtaattga ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac
    17641 tcgctgccag cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca
    17701 cagtctgaat gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt
    17761 ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg
    17821 tcggaatccc ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa
    17881 ctttcaccac gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt
    17941 cagcagtggt agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa
    18001 gttgcggaag cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg
    18061 tcacacccgc cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat
    18121 ccagatacag ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac
    18181 gggaaaactc tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa
    18241 tgtttgaacg aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg
    18301 atggccccgg tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt
    18361 gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc
    18421 tggaaggctg cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg
    18481 gtttgcttat tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct
    18541 gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga
    18601 ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg
    18661 gtgaaccctt catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag
    18721 gcattcatac tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc
    18781 tgccctatat cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac
    18841 agtggaccga cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg
    18901 gcaaaaaaat cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg
    18961 taaaagccat taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc
    19021 cctaccacac gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg
    19081 aaaaaccgct tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag
    19141 ggttaaccgc gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag
    19201 cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac
    19261 cgagcgcgcg cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt
    19321 acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga
    19381 tgagttgatc attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata
    19441 tactgtctcg tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt
    19501 tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca
    19561 gacggtacag gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac
    19621 cggcatcatt aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt
    19681 cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc
    19741 gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga
    19801 tatcatgctg gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat
    19861 ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg
    19921 cgatcttatc gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt
    19981 catccagttg attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga
    20041 tcagtatctc tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga
    20101 acacgccatc gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg
    20161 ctccggctcc cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg
    20221 cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat
    20281 cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc
    20341 aggaatgagt aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat
    20401 gccggcattc aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc
    20461 gcaggacgct tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg
    20521 gggctatcgc tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct
    20581 ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc
    20641 ggcaggtaac ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta
    20701 ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt
    20761 gcacgatatt ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa
    20821 gcaaggcggc gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg
    20881 caacagcctg gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca
    20941 gctggctgcc gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg
    21001 gctgattaac ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc
    21061 tcgcgcttat cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg
    21121 tggtccggtt ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt
    21181 tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg
    21241 cgctagtccg gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg
    21301 taaactgcct acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc
    21361 aacgctggag aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga
    21421 agtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc
    21481 gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt
    21541 cttcaccgcg ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct
    21601 gtaatctctt cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca
    21661 tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac
    21721 ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg
    21781 acggtacttt tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc
    21841 aggaactgaa aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc
    21901 ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac
    21961 tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga
    22021 ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa
    22081 gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc
    22141 atcgcctgaa acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc
    22201 tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg
    22261 gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac
    22321 ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg
    22381 tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg
    22441 cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata
    22501 atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca
    22561 gctgacctct ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat
    22621 cacatcaaat cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt
    22681 agcatagttt cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac
    22741 acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag
    22801 cttggcgatg tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct
    22861 gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat
    22921 atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg
    22981 gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac
    23041 tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg
    23101 acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag
    23161 gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt
    23221 gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc
    23281 cgccgccttc cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg
    23341 ctggaacgct ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat
    23401 ctggcgaaat acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg
    23461 gacaacacca ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat
    23521 atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta
    23581 ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga
    23641 agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt
    23701 ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc
    23761 cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc
    23821 gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg
    23881 ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac
    23941 ctcaacattc acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat
    24001 tgatgtggga ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct
    24061 taatgcctga tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata
    24121 ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc
    24181 agccgtcgtc accccgacag tatcggggtg acggataatc acttactcga tccctttact
    24241 acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag
    24301 aatgcgggtc gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc
    24361 ctgatacagt tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt
    24421 cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg
    24481 ccctttttcc ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag
    24541 caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact
    24601 catccattcg aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta
    24661 accaatgcgc gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac
    24721 cagcgttttc agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc
    24781 accgacctcc agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt
    24841 cagaccttcc acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa
    24901 cgggttctga cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc
    24961 gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg
    25021 caactcagca atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcttg
    25081 ggtcgccgcc gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc
    25141 cagatccgcc atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat
    25201 catggtgctg tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag
    25261 gttgttggtc ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag
    25321 aagcacacgc agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac
    25381 tggaattccc acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat
    25441 ttcgccgtat ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg
    25501 caaagcatag atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat
    25561 caccgtatcc agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat
    25621 gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat
    25681 cttcataaag gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc
    25741 gccgaatttg acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt
    25801 actggaaact aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat
    25861 aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa
    25921 tgtgatttcg tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca
    25981 atactctaat attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata
    26041 tgaaattgaa aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct
    26101 ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga
    26161 tcaccattcc tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg
    26221 ggctttccaa tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta
    26281 ctgtactgaa cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca
    26341 ttaacagtgc agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc
    26401 tgccgatcgg cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg
    26461 agcataagaa agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg
    26521 ctgcgggcga accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt
    26581 atgcactcta catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg
    26641 gcctgcgtgt aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg
    26701 agaaagtacc ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg
    26761 agccagacgg cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt
    26821 ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc
    26881 agccagatgt tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg
    26941 ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta
    27001 tttgccagac cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc
    27061 cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc
    27121 atacgtagcg caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt
    27181 accgcccatg ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc
    27241 acgttactga tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa
    27301 tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg
    27361 ataaacatcg ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc
    27421 gccagcagca caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc
    27481 agcgccgtca gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac
    27541 tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac
    27601 aacagcagga gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg
    27661 gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc
    27721 agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc
    27781 tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg
    27841 gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc
    27901 gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca
    27961 cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca
    28021 ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat
    28081 aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt
    28141 gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg tgctccatgc
    28201 cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag aaattttcga
    28261 ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca
    28321 cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagccgctta agcattttag
    28381 ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc cacctgacga gcttccccca
    28441 cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg cgccgatgcg
    28501 cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac tgcgtcactt
    28561 tttttgctat tggcgtacct gcgcgacgac tcattgtgct tcctcattat ggtgacgcat
    28621 acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt
    28681 aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta atgcaatcat
    28741 ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt caagaggtgt
    28801 atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc tatgttgtgc
    28861 gatatggtca acaacaaacc gcagcaagat aaaccttcag ataaatagcg gcgtcgcggt
    28921 acgccgcttc actcctgctt ttatgcaggc ataacgcgtt ttggtctgaa aaaaaccact
    28981 ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg
    29041 gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat gcggacccct
    29101 ttcccctctt cacgcactct tgcaggtatt gacccttgac gccagggtaa gcacatggcg
    29161 tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg
    29221 ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta acctcggctt
    29281 aggttacccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc ttttgctatg
    29341 gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg
    29401 ttatttccct tttgctcagg cctacggcgc gcctaatttc aatacattgc tggcattgca
    29461 ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt acagctacct
    29521 ggtcggctta tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa aagagaatga
    29581 aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg cgacattttt
    29641 tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag atactggcta
    29701 tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga ttgaagagca
    29761 agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg ccgttaagcc
    29821 gaagtatcag acctatgtgg tagtgatcgg tgaaagcgcg cgtcgcgatg ccctcggtgc
    29881 ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt tgatatttgc
    29941 tgactacatt gctgccagtg gctccacgca gaaatcgctt ggcttaacgc tcaatcgcgt
    30001 agtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt
    30061 ccagacctgg tggttttcca accagggcca aatcggcgaa tacgatactg ctatcgccag
    30121 catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg aagcagataa
    30181 aaacacgaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc aagagcactc
    30241 gcaaccgcag ctgattgttc tgcatctgat gggctcgcat ccgcaggcct gcgacaggac
    30301 acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat
    30361 gacgcaaacg gacgatttac tgcgcaagct gtacgatcag ttacgcaaca gcggcagcag
    30421 cttctctctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt
    30481 gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt ttatggtcat
    30541 ttccagcggc gataaagcgc atcgggtgat taaagcccgc cgctcagcca atgacttctt
    30601 aggctttttc tcgcagtgga cgggaattaa agcgaaggaa ataaatatca aatacccgtt
    30661 tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac agaaggtgga
    30721 ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaaacaaa aatccgcccc
    30781 gagaggcgga tttttatatc accaaagtga ttagaagcgg taaccaacac cggcaatcca
    30841 ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt
    30901 ttccatcggg ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg
    30961 tttgtaggtc gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta
    31021 gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt
    31081 tttgttgtag tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat
    31141 cacacccagc gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt
    31201 gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc
    31261 tacggaagta cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt
    31321 tttcataacc acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt
    31381 aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt
    31441 ccatacaccg ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac
    31501 gcatgttttc cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca
    31561 atcctggcgc attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat
    31621 tgggtatatc cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat
    31681 gcctggttca ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat
    31741 ggcgtctatt cagggtggag cctcgttagc taagtcactt tttcctctgg tgagcgcacc
    31801 gggtgtcact gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc
    31861 atggcgactg cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct
    31921 gggtgggatg aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt
    31981 ggcgctggag ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga
    32041 tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga
    32101 cgtttcccat gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc
    32161 tatttacatt ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtagcaat
    32221 tggttcgttg attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc
    32281 actctggcac tggtcggtta ttccattggg tctggctgtc gttattctct cgaccgctct
    32341 gccttattcg ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct
    32401 gatgagcatg gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact
    32461 gacacccata cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct
    32521 gacagtacgc aaagagagca aaataaaaga attagacatt aattaaattt acatttctgt
    32581 atggttatgc ataaccatgc agaatttctc gctacttttc ctctacaccg tatttatata
    32641 tcgaattatg caaaagcata tttattccga aaattcctgg cgagcagata aataagaatt
    32701 gttcttatca atatatctaa ctcattgaat ctttattagt tttgtttttc atgcttgtta
    32761 ccactattag tgtgatagga acagccagaa tagcggaaca catagccggt gctatactta
    32821 gtctcgttaa ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat
    32881 tagttaaatc aaaagcgacc aatctgcttt atacccgcaa cgatgtctcc gacagcgaga
    32941 aaaaagcaac agtagagttg ctgaatcgcc aggttatcca gtttattgat ctttctttga
    33001 ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt cattgccgta catgaaatgc
    33061 tggatggctt ccgcaccgca ctgatcgatc atctggatac catggcagaa cgtgccgtgc
    33121 agctgggcgg tgtagctctg gggaccactc aagttatcaa cagcaaaacc ccgctgaaaa
    33181 gttacccgct ggacatccac aacgttcagg atcacctgaa agaactggct gaccgttacg
    33241 caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc gaaagatgac gacaccgcag
    33301 atatcctgac cgccgcgtct cgcgacctgg ataaattcct gtggtttatc gagtctaaca
    33361 tcgaataaat ccatcgctga tggtgcagaa ctttagtacc cgataaaagc ggcttcctga
    33421 caggaggccg ttttgttttg cagcccacct caacgcactt atttagtgca tccatctgct
    33481 atccccagct gattaagtaa attttttgta tccacatcat cacacaatcg ttacataaag
    33541 attgtttttt catcaggttt tacgctaaat aatcactgtg ttgagtgcac aattttagcg
    33601 caccagattg gtgccccaga atggtgcatc ttcagggtat tgccctataa atcgtgcatc
    33661 acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa ttggcacgat tttttcatat
    33721 atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc tatgaagtct
    33781 gtattaaaag tttcactggc tgcactgacc ctggcttttg cggtttcttc tcatgccgcg
    33841 gataaaaaat tagttgtcgc gacggatacc gccttcgttc cgtttgaatt taaacagggc
    33901 gataaatatg tgggctttga cgttgatctg tgggctgcca tcgctaaaga gctgaagctg
    33961 gattacgaac tgaagccgat ggatttcagt gggatcattc cggcactgca aaccaaaaac
    34021 gtcgatctgg cgctggcggg cattaccatc accgacgagc gtaaaaaagc gatcgatttc
    34081 tctgacggct actacaaaag cggcctgtta gtgatggtga aagctaacaa taacgatgtg
    34141 aaaagcgtga aagatctcga cgggaaagtg gttgctgtga agagcggtac tggctccgtt
    34201 gattacgcga aagcaaacat caaaactaaa gatctgcgtc agttcccgaa catcgataac
    34261 gcctatatgg aactgggcac caaccgcgca gacgccgttc tgcacgatac gccaaacatt
    34321 ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga ctctctggaa
    34381 gcgcagcaat acggtattgc gttcccgaaa ggtagcgacg agctgcgtga caaagtcaac
    34441 ggcgcgttga aaaccctgcg cgagaacgga acttacaacg aaatctacaa aaaatggttc
    34501 agtactgaac cgaaataata acgctacacc tgtaaagcgc actggcagtt ccctctcccc
    34561 cctggggaga ggattagggt gaggggcgca aacccgctcc ggggccatta attaccctga
    34621 atttgattat ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc
    34681 tggcctgcca ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc
    34741 ggtctggctg gcggtctggt aatcggattg ctggcaggtt ttgcacgcac cttcggtggt
    34801 tggatagcca accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc
    34861 gtccaggtga tgtttatcta tttcgccctg ccgatggcgt ttaacgactt acgcatcgac
    34921 ccatttactg cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc
    34981 acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg aagcaggact ggcgctcggt
    35041 ctttcacgtt gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg
    35101 ccgccgctgg gtaaccagtg gatcatcagc attaaagaca cctcgctgtt tattgttatc
    35161 ggcgtggcgg aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt
    35221 gagatctgga gcgccgtggc ggtgttctat ctgattatta ccctggtgct gagctttatt
    35281 ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc
    35341 actttggccc aacccaggtg ctgcacaata tcgatttgaa cattgcccag ggcgaagtcg
    35401 tggtgattat cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac
    35461 tggaagaaat cacctccggc gatctgattg tcgatggcct gaaggtcaac gatccgaaag
    35521 ttgacgagcg cctgattcgc caggaagcag gtatggtgtt ccagcagttt tacctcttcc
    35581 cgcatctgac ggcgctggga aacgtcatgt ttggtccgct acgcgtgcgt ggcgcgaaca
    35641 aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg gcagaacgtg
    35701 cacatcacta cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg
    35761 cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg
    35821 aactgcgcca tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg
    35881 tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc ttcgcggctg atctttatcg
    35941 acaaaggccg gattgcggaa gatggcaatc cgcaggtgtt gatcaagaac ccgccgagcc
    36001 agcgcttgca ggaatttttg cagcacgtct cttaataaga cacattgcct gatgcgctac
    36061 gcttatcagg cctacaggat atctggcaac ttattaaaat tgcatgaact tgtaggacgg
    36121 ataaggcgtt cacgccgcat ccggcaaaaa agcccgcacg ttgtcagcaa cctgcttaat
    36181 atcccttcct ccctttcacc cgaaagggag gcacaccaga ttcctctcat ttaaaatcgc
    36241 ccctccccca gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg
    36301 gtggatcctg ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc
    36361 cggcgttaca accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat
    36421 cgaacaaaaa aagcggccta tggcgcactg gcggatgtgc tggataacga cacctcgcgt
    36481 aaagagttga tcgaccagtt gcgtactgtc gccgcgacgc cccctgcgga accggtcccg
    36541 aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt caccgaagtc
    36601 agccgccatt atggtgaagc cctttccgcc cgcttcgggc aactttatcg caatatcacc
    36661 ggctccccgc ataagccatt taatccacaa accttcagca atgcgctgag ccatttttca
    36721 atgttagcgg tattagtgtt tggtttttac tggctgattc gcctgtgcgc actgccgcta
    36781 tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg agcgcagcaa ctggttgcag
    36841 cttccggcga tgattattgg ggcgtttatt atcgacctgc tgttactggc cctgacatta
    36901 tttgtcggcc aggtattaag cgacaacctg aatgcaggca gtcgcaccat cgctttccaa
    36961 caaagtttgt ttctcaacgc ctttgccctg attgaatttt tcaaagccgt actacgcctg
    37021 attttttgcc caaacgtggc ggagctgcgc ccgttcacga ttcaggacga gagcgcccgt
    37081 tactggggtc gtcgcctgag ctggttaagc agcctgattg gctatggcct gattgtggcc
    37141 gtgccgatta tctctaatca ggtgaatgta cagataggtg cgctggcgaa cgtcatcatt
    37201 atgctgtgca tgaccgtctg ggcgttgtac ctgatcttcc gaaataaaaa agagattacc
    37261 cagcatatgc tcaactttgc ggagcattcg ctggcttttt tcagcctgtt tatccgcgcc
    37321 tttgcgctgg tgtggcactg gctggcaagc gcctatttta tcatgctgtt tttcttttcg
    37381 ttgttcgatc cgggcaacag tctgaaattt atgatgggtg caacggtgcg cagcctggcg
    37441 attattggta tcgcagcgtt tgtttccggt atgttttccc gctggctggc gaaaaccatc
    37501 accctctcgc cacatactca gcgtaactat ccggagctac aaaaacggct gaatggctgg
    37561 ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggcagt aatgttgctg
    37621 ttgagcgcat ggggattgtt cgatttctgg aactggctgc aaaacggcgc ggggcagaaa
    37681 accgtagata tcctgatccg catcgcactc attcttttct tctcggcggt tggctggacg
    37741 gtgctcgcca gtttgatcga aaaccggctg gcttcggata ttcatggccg cccgctaccc
    37801 agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg cgctggcggt gattatcagt
    37861 accatcacca tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc attgctggca
    37921 ggtgccgggg cattaggtct ggctatctcg tttggttcgc aaacgctggt aaaagatatt
    37981 atcaccgggg tatttattca gtttgaaaac ggcatgaaca ctggagattt ggtgactatc
    38041 gggccgttga ccggcacagt ggaacggatg tcgattcgct ccgtgggcgt gcgccaggat
    38101 accggggcgt atcacatcat tccgtggtct tcgataacca cctttgctaa cttcgtccgc
    38161 ggtattggtt cggtagtggc aaactacgat gttgatcgcc atgaagatgc tgataaagcc
    38221 aatcaggcac tgaaagatgc ggtaacggaa ttgatggaaa acgaagaaat tcgcgggctg
    38281 attattggtg aaccgaactt tgccgggatt gtcggcttaa gcaataccgc gtttacactg
    38341 cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg tacgctttgc cctcgacagc
    38401 caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc cagtgcagac ttatcaggtg
    38461 ctgcctgctc cgggcgcgac cccggctgaa ccgctgccgc cgggggagcc aacgctttaa
    38521 cgttggcgat taacaaaacg gcggcgctgc tcgtcgttca taaaggtcca ggcaataaag
    38581 cgactctgct tttgcccctg ggccatctct ttttttacca ccttcaccgc gcccacgtcg
    38641 gtcagagcac gatacaacgg cggtaagttt tcaccacgag ataccagcga ggtaaaccac
    38701 atcacctgct tcgcgaagcc tttgctctct tcaatcatct ttttgataaa gttgacttca
    38761 ccgccttcac accacaactc ctgttgctga ccaccaaagt tcagtgcatc gtctttgttc
    38821 agccccaggt tacggcgttt acgctcacta cctgcccgcg ccgcagcggc ggaatcgtgg
    38881 aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt tgtggatgat gccgttaaaa
    38941 atcgccccac tctctttttg ccgacgcaga cgaatggcgc ggttaagccc cggattagca
    39001 ctgataatcg cctgcgcact gcttaacgcc tggctgctgg tttcactacc ggtaaaacgc
    39061 cagccatatt catgtacgcc aatcagcgga taaatacagt tcgcaccaac gccgatatcc
    39121 agaatgctgg cattcgctgg aattgttccg cttgcttcag ccagtaaatc ggcaaggtga
    39181 tgaatataat ccgcccggcc cggtactggt gggcagagaa aaccgtcggg gatatcccag
    39241 ttcgctacgg cgtaaaaatg ggccagcaac gccttattga gcgccttcac cgccagcgga
    39301 ttggcaaagt ctacgctttg ctcccctgcg ggtgtaagcg tgaggaattg cctgagttca
    39361 ggattgacct gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt gcgcggatgc
    39421 aaccccggtt tctgggcgga catagcattc tcctttaagc agcggcgtaa gatacccgca
    39481 taacgcgaca gggtaaatat ctggctgcgt ttatcattcc ccaaaacctt aacatggcta
    39541 tggtttatgg caattcatca taaaaatcag cattatgcat gtcattgatt tgtcttattt
    39601 tgtaaaaata tatcgttcaa aaaataagca tttctctgtc aattttttgc gtcatttgca
    39661 tcgcgcaggc gacctacact tatcaggcac tacccgttgt atttatgagg atgtcattat
    39721 gaaaaagtgc ctcacactac tgattgccac cgtcctgagc ggaatctctc tcacggctta
    39781 tgccgcgcaa ccgatgagta acctggacag cggtcagcta cggcccgccg gtaccgtttc
    39841 ggcaactggc gcatcaaacc taagcgatct ggaggataaa ctggcagaaa aagcgcgcga
    39901 acaaggcgcg aaaggttatg tcattaattc cgcaggcgga aatgaccaga tgttaggtac
    39961 tgcaaccatc tacaaataac cccaccaacg taactgcatt cgcctctgga tgcagttttc
    40021 gcctgttttg taatcaaaaa acatcaaata tgaacgcaat gtaaataaat gtatttcttt
    40081 ttcgcgcaat gggtgacaga aaatcgcatc aagtgataat gcttatcaaa attattatca
    40141 cttgcacgaa cactatcacg ggattaacag tggcatcgca tccgcagaga tgctttctcg
    40201 tggcagtgaa aatttcaaca tataagaaaa agtcacctgc aaaatggaaa acaatcgcaa
    40261 tttccctgcc agacaatttc attcgctcac gttctttgcc ggtctttgta ttggtattac
    40321 gcctgtggca caggcactcg ccgccgaagg gcaagctaac gcggatgaca cgctggttgt
    40381 cgaagcatca acgccttcgc tttatgcgcc acaacaatct gccgatccga aattctcgcg
    40441 tccggtagcg gatactaccc gcacgatgac ggtgatttct gaacaagtga ttaaagatca
    40501 gggcgcaacc aaccttaccg acgcgctcaa aaacgtcccc ggcg
//
LOCUS       DAZOYF010000017        39978 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZOYF010000017 DAZOYF010000000
VERSION     DAZOYF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.017"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..155
                     /locus_tag="ACHUOF_000884"
     CDS             <1..155
                     /locus_tag="ACHUOF_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0610174.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="ACHUOF_000885"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="ACHUOF_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR0610175.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="ACHUOF_000886"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="ACHUOF_000886"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR0610176.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="ACHUOF_000887"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="ACHUOF_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="ACHUOF_000888"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="ACHUOF_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR0610177.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="ACHUOF_000889"
     CDS             complement(5626..7134)
                     /locus_tag="ACHUOF_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR0610178.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHUOF_000890"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHUOF_000890"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR0610179.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="ACHUOF_000891"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="ACHUOF_000891"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0610180.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="ACHUOF_000892"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="ACHUOF_000892"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR0610181.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHUOF_000893"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHUOF_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR0610182.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHUOF_000894"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHUOF_000894"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR0610183.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="ACHUOF_000895"
     CDS             complement(14283..15602)
                     /locus_tag="ACHUOF_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFR0610184.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHUOF_000896"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHUOF_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR0610185.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="ACHUOF_000897"
     CDS             16699..17034
                     /locus_tag="ACHUOF_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR0610186.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHUOF_000898"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHUOF_000898"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR0610187.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="ACHUOF_000899"
     CDS             complement(17790..19172)
                     /locus_tag="ACHUOF_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0610188.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHUOF_000900"
     CDS             complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHUOF_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR0610189.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="ACHUOF_000901"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="ACHUOF_000901"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR0610190.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="ACHUOF_000902"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="ACHUOF_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR0610191.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHUOF_000903"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHUOF_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR0610192.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHUOF_000904"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHUOF_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR0610193.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHUOF_000905"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHUOF_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR0610194.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="ACHUOF_000906"
     CDS             complement(25947..26768)
                     /locus_tag="ACHUOF_000906"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR0610195.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="ACHUOF_000907"
     CDS             complement(26868..26951)
                     /locus_tag="ACHUOF_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610196.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHUOF_000908"
     CDS             complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHUOF_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR0610197.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
     gene            27623..27727
                     /locus_tag="ACHUOF_000909"
     CDS             27623..27727
                     /locus_tag="ACHUOF_000909"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR0610198.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(27776..29029)
                     /locus_tag="ACHUOF_000910"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="ACHUOF_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30038
                     /locus_tag="ACHUOF_000911"
     CDS             29136..30038
                     /locus_tag="ACHUOF_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0610199.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30173..31393
                     /gene="mlc"
                     /locus_tag="ACHUOF_000912"
                     /gene_synonym="dgsA"
     CDS             30173..31393
                     /gene="mlc"
                     /locus_tag="ACHUOF_000912"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR0610200.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31518..32213
                     /gene="bioD"
                     /locus_tag="ACHUOF_000913"
     CDS             31518..32213
                     /gene="bioD"
                     /locus_tag="ACHUOF_000913"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0610201.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="ACHUOF_000914"
     CDS             complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="ACHUOF_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR0610202.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="ACHUOF_000915"
     CDS             complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="ACHUOF_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR0610203.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34274..35128)
                     /gene="ynfH"
                     /locus_tag="ACHUOF_000916"
     CDS             complement(34274..35128)
                     /gene="ynfH"
                     /locus_tag="ACHUOF_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR0610204.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35130..35747)
                     /gene="ynfG"
                     /locus_tag="ACHUOF_000917"
     CDS             complement(35130..35747)
                     /gene="ynfG"
                     /locus_tag="ACHUOF_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR0610205.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35758..37796)
                     /locus_tag="ACHUOF_000918"
                     /pseudo
     CDS             complement(35758..37796)
                     /locus_tag="ACHUOF_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(37857..38891)
                     /locus_tag="ACHUOF_000919"
                     /pseudo
     CDS             complement(37857..38891)
                     /locus_tag="ACHUOF_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            38949..>39978
                     /locus_tag="ACHUOF_000920"
     CDS             38949..>39978
                     /locus_tag="ACHUOF_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR0610206.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVE"
BASE COUNT        10164 a         9697 c        10236 g         9881 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctag
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg actggactgt
    29761 atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc actcaggagg
    29821 tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt tcaattttgc
    29881 ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt gctgaagagg
    29941 atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt ccagctgcgc
    30001 gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag cgaaaaagcc
    30061 cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt attgaagtgc
    30121 ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg cggtggttgc
    30181 tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg cggtttatcg
    30241 cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg cgcagctggc
    30301 tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg tgcgagagct
    30361 ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg ttgaaactga
    30421 agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg ctctgcgcga
    30481 tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag atgacttgcc
    30541 attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc agaaaaaact
    30601 tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg aaaatggtat
    30661 tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg aggcgctgga
    30721 gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga cgatggcaga
    30781 ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta tcgatcacaa
    30841 cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca gtagtctcgt
    30901 ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg ggaaccacgg
    30961 ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc tgcgtctcaa
    31021 tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat tgtgtcaggc
    31081 ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg cgcatgtagg
    31141 gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga ttggctcacc
    31201 gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc gtcagcaggc
    31261 ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta accagggcac
    31321 gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt tgattcgtct
    31381 gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc aatttgggcc
    31441 aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat ttgtgaagca
    31501 taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac acttctgtag
    31561 ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga aaaagggttg
    31621 cgggatataa acccgtagcg aaggggagca aagagacacc cgaagggctg cgtaataaag
    31681 atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca gttaatccta
    31741 tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat tacaccctca
    31801 tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta gaagggactg
    31861 gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta gtgcaggaac
    31921 aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat gcactgctaa
    31981 cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct aaccgaatca
    32041 acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa cttccggcac
    32101 cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg gggcaataca
    32161 tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg taacgtccgc
    32221 gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg atactccccg
    32281 gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc cgccaacagt
    32341 gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg gaaccataat
    32401 cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt cggtgtaaag
    32461 acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt acaaaggaag
    32521 atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac ggtgctatag
    32581 ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa cccgcctagt
    32641 gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca ggcgttcatt
    32701 aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat aatcagcgca
    32761 tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc gtcgctatga
    32821 ttaatcagat tgctaaccag ccatgccacg atggcggaaa taatcaccgg gccgagagag
    32881 gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc aagcggggca
    32941 cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt ccattcctgg
    33001 cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag aatcatcgca
    33061 ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag cgatttaacc
    33121 aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta atcggtcggc
    33181 gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc cgccgccagt
    33241 ccgccgagcg ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc tgcattgacc
    33301 agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc atgacgaaac
    33361 ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag acggcggaac
    33421 atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg gcaatgaaac
    33481 acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg ctttaagaga
    33541 taaaaaaacg tgacattgta acgtattatt gccgaatcgt gcgtaatcgt caccatccgg
    33601 caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc aacagttgtg
    33661 attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag aaagggtgtt
    33721 ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg tgccatgcca
    33781 gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc atcaacagca
    33841 gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg cctttctcgc
    33901 gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg cgatccagcc
    33961 agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa cgctgccagg
    34021 cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc gctaatgacg
    34081 cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca accagaggtg
    34141 cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc gccgcgacag
    34201 aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt gcgggttgcc
    34261 ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg ccataaaaga
    34321 gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca agtccggcaa
    34381 cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct gcgcataaca
    34441 ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg gcttgttgca
    34501 cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg gcacaagcga
    34561 tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg cgggctgccc
    34621 gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag aaagccagcg
    34681 tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag gtcatcatcc
    34741 agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc aacgcttgcg
    34801 gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa aatatggaac
    34861 cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag ttaaacgctc
    34921 gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc aacagccaga
    34981 ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg gcgaaccagc
    35041 caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta aagataacca
    35101 gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc cagataacca
    35161 gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt gggttttgtg
    35221 aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg gcgcagctct
    35281 tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat gggttgtttc
    35341 ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt ttcagcattg
    35401 tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat acaaacatct
    35461 tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg gcagactttt
    35521 gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc gaacacgttc
    35581 tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata aatacggcgg
    35641 aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca ggttttacag
    35701 ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg ctactcctta
    35761 aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcca gcggtgaagg
    35821 gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac caaacatatc
    35881 cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac gcggagtcac
    35941 ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat cgccatgacg
    36001 gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc aggcctgctg
    36061 taacacatca atattgccgt agctggagtg ggtacgcgct ttggtttggc gacttcactc
    36121 agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg ggcgaggatc
    36181 acatagccca tattccctgc agattcatga gagataaggt cttcttgctc tgttggcatc
    36241 aggtcaggca acaggatatc gcaatactta gccgaggccg tcatgaagtg gtcaatgcca
    36301 acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt gatgtcgcca
    36361 tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg gacatccagt
    36421 ttttcttttc cacgaacacc atcacgggtc gcggtcattt tcgtaccatg gtcgatggca
    36481 tctgtccatg taaaaacgga aatctgcgtt ttaacaggat tctcgagcat cgggaaccat
    36541 tctaccccca gatcccagct accttcgcgt acacctgagt tgccgccgtt tatgccgacg
    36601 ttaccggtaa gaacggaaag catggcaata gcgcgggacg tttgttcgcc gttggagtgt
    36661 cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat ctctcgtgcc
    36721 aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc cggcgtttta
    36781 gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt acgtggtgcg
    36841 ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa tggctgatcg
    36901 accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc atcggtgcca
    36961 gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata gcgtggatcg
    37021 atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta agtgacacca
    37081 ccgccgctca tccgcgtttc tgccgggtta tttccgaaca taacgaccag tttcgtattg
    37141 gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat tgcggcactg
    37201 atctgtgcgg tactgtagct gccatagcga ctgagaaaac ccccgcaaga gttcatcaga
    37261 cggtacggga cgttcgagtt ggtgatattt ccgccatcta cgcctgttcc gtacaggaca
    37321 tgtacagcct cattgccgta atctttcagg atccgccgaa gattatcact gatggtatcc
    37381 agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgctcgcc gacgcgcttc
    37441 atgggatatt tcaacctgtc aggatgattc atccgtcggc ggatagagcg cccgcgtaaa
    37501 catgcccgaa cctgatgatt accgtaaacg tcgtcacctg tcgtatcaga ctccacccag
    37561 tacacggtgt catctttcac atgcaaacgt agcagacagc ggctcccgca gttaacggtg
    37621 caggaactcc agaccgtttt ctcttctacc ggagcctctg ccgcccggac catttgggaa
    37681 aatggcagag tgaaagcact gcttgccagc gcgagactgc caagtgcgga ggttttcatc
    37741 agacttctat ggctgatttc agccttcatg agcgcctctg tggtatggat tttcatcatt
    37801 actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt agtgggttat
    37861 attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag tggtgaaggg
    37921 cgcagagtgg ttagcgtatt cacacatccg ccatggtcga ttttatcgcc agacatattg
    37981 ggctcgtgcc aggctccctg gcccatagcg ctaactccag ggagaatgcg tggtgttact
    38041 ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc gccgttggca
    38101 atcccacgtt tctgtgcatc tatagggttg atccacacct cctgacggca ggctgccttc
    38161 aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa accaaacagt
    38221 tgcagtggga aggttctacg ttcaggggag tcccagcctt caaaggttga ggcataaact
    38281 ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat ttccgccagc
    38341 ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc ctcggggtcg
    38401 tcacgaaatg ctttgtaggc gacaaaatgg ccattgggat ctttacgctt atagataccc
    38461 atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat tttggcgtac
    38521 agatgttgta accattgttc ctgcgtgcga ccttctgtga acttttgata gacgtcaggt
    38581 ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttacgttc gaatttttcg
    38641 ctggtgactg gctggaggaa aatgagatat cccatgttac cggcgtagtc gttaggaata
    38701 atatcttcct gctcaacggt catcaggtct tccagcagaa tgtcggcata ttttgccgat
    38761 gaggtcataa agttttcgat gaccacaatc atttcgcatt tcgattcgtc ctgcagaatt
    38821 tcatgcgttt tgttgatgtc agaatgctga ttaacgaggg tatttcccgc gtagttccag
    38881 atggactcca ttgccagacg gatggatgga cttgaagtag ttgactcaat gctacttaag
    38941 gtcctgatat gactgacatt aatcatgata acactgtata tccatccagt tctttctctg
    39001 ggtctcccta tcggtatcag caagtctaca cctcaagccc tgacacaagg ctgacacaaa
    39061 tcaatctact cagcgtagct aagcctcagt tagtccgcag agccaatggt cgatatacta
    39121 tccgcttcag actcaaggga cagaccacac cgtttctttc agtatcgacc cgcagcacag
    39181 acaggagagt agccacaatg cgtcagagag agcttgcagc caccgctaag gccttcatgt
    39241 tggataggcc tgaagtctca ctacaggagc ttacagagca tctccggtct atggctgagc
    39301 agttcctgac agacgctagt gacgattact ggaatggact tgaggtcgct actctggtcg
    39361 acgagaagtc caacctgaag gagctagccg ctacgcaagc cttgagtctg gaccagcaga
    39421 agggcataag gttagcgctg gaagtcctga ccgcagcaca gcagagagtc gacactggtg
    39481 atacctctgg tctgattaag ctgattgatg ataataacct tactgatgac tcaactatag
    39541 gcgactctac gtcaattctg aataatgagc agggggatag accggcagtt ttcacacagg
    39601 agcgccagtc atctgtagtt ttttcaagcc tagtgtcctc gctgctggct gagaaggtcc
    39661 agaccctgaa gacatccagc tacaaggacc tctcgtcctc actcaatact gtctcccggt
    39721 tcctgcctga agacatggac ctgatgtccc gctctgggtg gctggctgtc agggactcta
    39781 tgctggcttc tgaggtgaga ccatcgacaa tcaacaagct gctgaccaaa gcgaagatgt
    39841 gtctcgatta tggcctgatg aatgggcaac ttgagggccg caaccctatt gaacggatga
    39901 agctgaccaa ggatatcgac tccaagcgca gagcgttcac agacgaggag ctggagagac
    39961 ttctggtccg tgttgagt
//
LOCUS       DAZOYF010000018        37571 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZOYF010000018 DAZOYF010000000
VERSION     DAZOYF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.018"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(24..278)
                     /locus_tag="ACHUOF_000921"
     CDS             complement(24..278)
                     /locus_tag="ACHUOF_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR0610207.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="ACHUOF_000922"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="ACHUOF_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR0610208.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="ACHUOF_000923"
     CDS             1228..2694
                     /locus_tag="ACHUOF_000923"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0610209.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHUOF_000924"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHUOF_000924"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR0610210.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHUOF_000925"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHUOF_000925"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR0610211.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="ACHUOF_000926"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="ACHUOF_000926"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR0610212.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="ACHUOF_000927"
     CDS             5709..7265
                     /locus_tag="ACHUOF_000927"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR0610213.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="ACHUOF_000928"
     CDS             7347..9161
                     /locus_tag="ACHUOF_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR0610214.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="ACHUOF_000929"
     CDS             9162..10256
                     /locus_tag="ACHUOF_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR0610215.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="ACHUOF_000930"
     CDS             10256..11281
                     /locus_tag="ACHUOF_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0610216.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="ACHUOF_000931"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="ACHUOF_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR0610217.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="ACHUOF_000932"
     CDS             complement(12876..13220)
                     /locus_tag="ACHUOF_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR0610218.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14749)
                     /gene="bcr"
                     /locus_tag="ACHUOF_000933"
     CDS             complement(13553..14749)
                     /gene="bcr"
                     /locus_tag="ACHUOF_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFR0610219.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVL
                     VWLSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVL
                     SYMLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVR
                     RIGALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVIL
                     DEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYA
                     SRPKKR"
     gene            complement(14777..15472)
                     /gene="rsuA"
                     /locus_tag="ACHUOF_000934"
     CDS             complement(14777..15472)
                     /gene="rsuA"
                     /locus_tag="ACHUOF_000934"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR0610220.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15622..17382
                     /locus_tag="ACHUOF_000935"
     CDS             15622..17382
                     /locus_tag="ACHUOF_000935"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR0610221.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17507..17791
                     /gene="rplY"
                     /locus_tag="ACHUOF_000936"
     CDS             17507..17791
                     /gene="rplY"
                     /locus_tag="ACHUOF_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR0610222.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17930..18937)
                     /gene="yejK"
                     /locus_tag="ACHUOF_000937"
     CDS             complement(17930..18937)
                     /gene="yejK"
                     /locus_tag="ACHUOF_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR0610223.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19119..19346
                     /locus_tag="ACHUOF_000938"
     CDS             19119..19346
                     /locus_tag="ACHUOF_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR0610224.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19366..21126
                     /gene="yejM"
                     /locus_tag="ACHUOF_000939"
     CDS             19366..21126
                     /gene="yejM"
                     /locus_tag="ACHUOF_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR0610225.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21201..21277
                     /locus_tag="ACHUOF_000940"
     tRNA            21201..21277
                     /locus_tag="ACHUOF_000940"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21235..21237,aa:Pro,seq:ggg)
     gene            complement(21380..23971)
                     /gene="yejO"
                     /locus_tag="ACHUOF_000941"
     CDS             complement(21380..23971)
                     /gene="yejO"
                     /locus_tag="ACHUOF_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFR0610226.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24291..24938
                     /gene="narP"
                     /locus_tag="ACHUOF_000942"
     CDS             24291..24938
                     /gene="narP"
                     /locus_tag="ACHUOF_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR0610227.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25149..26201)
                     /gene="ccmH"
                     /locus_tag="ACHUOF_000943"
     CDS             complement(25149..26201)
                     /gene="ccmH"
                     /locus_tag="ACHUOF_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR0610228.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26198..26755)
                     /gene="dsbE"
                     /locus_tag="ACHUOF_000944"
     CDS             complement(26198..26755)
                     /gene="dsbE"
                     /locus_tag="ACHUOF_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR0610229.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26752..28695)
                     /gene="ccmF"
                     /locus_tag="ACHUOF_000945"
     CDS             complement(26752..28695)
                     /gene="ccmF"
                     /locus_tag="ACHUOF_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR0610230.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28692..29171)
                     /gene="ccmE"
                     /locus_tag="ACHUOF_000946"
     CDS             complement(28692..29171)
                     /gene="ccmE"
                     /locus_tag="ACHUOF_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR0610231.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29168..29377)
                     /gene="ccmD"
                     /locus_tag="ACHUOF_000947"
     CDS             complement(29168..29377)
                     /gene="ccmD"
                     /locus_tag="ACHUOF_000947"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR0610232.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29374..30111)
                     /gene="ccmC"
                     /locus_tag="ACHUOF_000948"
     CDS             complement(29374..30111)
                     /gene="ccmC"
                     /locus_tag="ACHUOF_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR0610233.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30153..30815)
                     /gene="ccmB"
                     /locus_tag="ACHUOF_000949"
     CDS             complement(30153..30815)
                     /gene="ccmB"
                     /locus_tag="ACHUOF_000949"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR0610234.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30812..31435)
                     /gene="ccmA"
                     /locus_tag="ACHUOF_000950"
     CDS             complement(30812..31435)
                     /gene="ccmA"
                     /locus_tag="ACHUOF_000950"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR0610235.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31448..32050)
                     /gene="napC"
                     /locus_tag="ACHUOF_000951"
     CDS             complement(31448..32050)
                     /gene="napC"
                     /locus_tag="ACHUOF_000951"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR0610236.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32060..32509)
                     /gene="napB"
                     /locus_tag="ACHUOF_000952"
     CDS             complement(32060..32509)
                     /gene="napB"
                     /locus_tag="ACHUOF_000952"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR0610237.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32506..33369)
                     /gene="napH"
                     /locus_tag="ACHUOF_000953"
     CDS             complement(32506..33369)
                     /gene="napH"
                     /locus_tag="ACHUOF_000953"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR0610238.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33356..34051)
                     /gene="napG"
                     /locus_tag="ACHUOF_000954"
     CDS             complement(33356..34051)
                     /gene="napG"
                     /locus_tag="ACHUOF_000954"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR0610239.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34058..36544)
                     /gene="napA"
                     /locus_tag="ACHUOF_000955"
     CDS             complement(34058..36544)
                     /gene="napA"
                     /locus_tag="ACHUOF_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR0610240.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36541..36804)
                     /gene="napD"
                     /locus_tag="ACHUOF_000956"
     CDS             complement(36541..36804)
                     /gene="napD"
                     /locus_tag="ACHUOF_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR0610241.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36794..37288)
                     /gene="napF"
                     /locus_tag="ACHUOF_000957"
     CDS             complement(36794..37288)
                     /gene="napF"
                     /locus_tag="ACHUOF_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR0610242.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37397..37561
                     /gene="yojO"
                     /locus_tag="ACHUOF_000958"
     CDS             37397..37561
                     /gene="yojO"
                     /locus_tag="ACHUOF_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR0610243.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
BASE COUNT         9171 a        10158 c         9279 g         8963 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccgcagcc agcccgtgga agaaacgcat cacaatcagc tgatcgatgg
    14461 tttgtgccaa cgcacacgcc accgcggcgg cggcaaacac cagcgtcccg ccaagaacca
    14521 ccggcttacg cccgaagctg tctgccatcg gcccgtagat taactgcccc aacgcaaagc
    14581 ccagaatata agtactgagg gtcatctgcg tactgcccgc cgatacgcca aactgcgctg
    14641 aaattaccgg tagcgcgggc agatacatat caatcgacag cggcatcaac atggccagca
    14701 ggccaaggat aaaaacaata gcaaacgacg aatgctgtcg ggtggtcaca acgggctcct
    14761 gaaagtcatt gaaaagttag acgacgctgg caatttcttc ttcagttaac ggacgatatt
    14821 caccgggggc taaatcagca tccagcgtaa taccgccaat acgttcacga tgcagctcaa
    14881 ccacgtggtt acccacggcg gcgaacatgc gtttcacctg atgataacgc ccttcgctga
    14941 tggtcagacg aacctgcgtt ggggtaatca cttcaagcac cgccggctta gtgagatctt
    15001 tttcgttatg cagctgcacg cctttagcaa attgctctgc cgtatcgtca gctacaggtg
    15061 attccagtgt caccagatag gtcttctcgc aatgatggcg cggagaagta atgcggtgcg
    15121 accactgacc atcatcagtc atcagcacca gaccggtggt atcgatatcc agacgtcctg
    15181 cggcgtgcag tttccacgct accggttcat ccagaaaata gagcaccgtc gggtgatccg
    15241 ggtcgtccgt ggagcaaaca tagccctgag gcttattgag catgaagtaa cgtggaccgt
    15301 gttgctgcgc cagcgggttg ccatcgtaag cgacatcatg ttcagggagc agtttgaacg
    15361 ctgcattacg aacgatttcg ccatcgacgg tgacacgatt gccgcggatt tcacgcccgg
    15421 caatagcacg gctaacgccg agttgctgtg cgataaattt atcaagtcgc atgtgtgtga
    15481 ttttgcctgt aaaaaacgga ggtcgggtaa tgacccgaaa atctgaactg ttgtctgccc
    15541 agtatagcgg tctaataact tccctcaagg gaaaaagaac cgtggcatac tattagcaga
    15601 ataatctacc tacgcgaaac catgattttt acactccgcc catatcagca agaagccgtg
    15661 gatgccacgc tcaaccattt tcgtcgtcat aaaacccctg ccgttatcgt gctgcccacc
    15721 ggcgcaggta aaagcctggt gatagcggaa ctggcacggc tggcgcgtgg tcgcgtactg
    15781 gtgctggcac acgttaaaga actggtggcg caaaaccatg caaagtatca ggcgctgggg
    15841 ctggaagccg atatttttgc cgccgggcta aagcgcaaag agagccacgg taaagtggta
    15901 tttggcagcg ttcagtcggt cgcccgtaat cttgatgcct ttcagggtga attttcgctg
    15961 ttgattgtcg atgaatgcca ccgtattggt gacgatgaag agagccagta tcagcaaatc
    16021 ctcactcacc tgactaaagt gaatccccac ttacgcctgc tggggctgac tgccacgcct
    16081 tttcgattgg gcaaaggctg gatctaccag ttccattatc acggcatggt acgcggcgat
    16141 gagaaagccc ttttccgtga ctgcatttat gagctgccgc tgcgttatat gattaaacac
    16201 ggctatctga cgccgccaga acgactggat atgccagtag tgcaatacga tttcagccgc
    16261 ttgcaggcac agagtaacgg gctgttcagc gaagctgatc tcaaccgtga gctgaaaaaa
    16321 cagcaacgca ttacgccaca tatcatcagc cagatcatgg agttcgccga aaagcgcaaa
    16381 ggggtaatga ttttcgccgc caccgttgaa cacgcaaaag agattgtggg attactgcct
    16441 gccgaagatg ctgcactgat tactggcgac aaccccggcg ctgagcgcga tgtgttaatt
    16501 gaagatttta aagcccagcg ttttcgctat ctggtcaacg tcgcagtact gaccaccgga
    16561 tttgacgccc cgcacgtcga tcttatcgcc attctgcgcc ctaccgaatc agtgagtctt
    16621 taccaacaaa ttgtcgggcg cggtctgcgt ctcgctccgg gcaagactga ttgtttaatt
    16681 cttgattatg cgggtaatcc tcacgatctc tacgcgccgg aagttggtac accgaaaggc
    16741 aaaagtgaca acgttccggt acaggttttc tgccctgcct gcggttttgc caacaccttt
    16801 tgggggaaaa cgaccgccga cgggacattg attgaacact ttggtcgccg ctgtcaggga
    16861 tggtttgaag atgacgacgg tcatcgcgaa cagtgtgact tccgtttccg ttttaaaaat
    16921 tgcccgcaat gtaacgcaga aaatgatatt gccgctcgcc gctgccgcga atgtgacacg
    16981 gtactggttg atccggatga tatgttaaaa gcggcgctac gactgaaaga tgcgctggta
    17041 ttacgctgta gcggcatgtc tttgcagcat gggcacgacg agaaaggcga atggttgaaa
    17101 atcacctatt acgatgaaga cggcgcggat gtgagtgagc gtttccgtct gcaaacgccc
    17161 gcccagcgta ccgccttcga gcagcttttt atccgcccgc atacgcgcac accgggcatc
    17221 cctctgcgct ggatcaccgc cgccgatatc ctcgcccagc aagccttact gcgacatccg
    17281 gattttgtcg tcgcccgcat gaaaggtcag tactggcaag tgcgtgaaaa agtgttcgat
    17341 tacgaaggtc gttttcgtcg ggcgcacgaa ttacgcggtt aatggtagtt ttgattgatg
    17401 tataaggcga ttgagtatag aatctcgccc gcttttgcat acgcaaagca gatcacttac
    17461 ctgttgctgg gtcgcctgta gcaggattaa tttaagagag aaagaaatgt ttactatcaa
    17521 cgcagaagta cgtaaagagc agggtaaggg tgcgagccgc cgcctgcgtg ccgctaacaa
    17581 gttcccggca atcatctacg gtggcaaaga agcaccgctg gctatcgagc tggatcacga
    17641 caaagtcatg aacatgcaag ctaaagctga attctacagc gaagttctaa ccatcgttgt
    17701 tgacggtaaa gaaatcaaag ttaaagctca ggacgtacag cgtcacccgt acaaaccgaa
    17761 gctgcagcac atcgacttcg ttcgcgctta attgctgaat aagttgtaaa aaaccccgct
    17821 ccggcggggt tttttgtatc tgcagattat gcctgattac ggtattgcta ttttttgcag
    17881 gccagataag gcgttcacgc cgcatctggc attcagaaca caaaaccgat taattgccgc
    17941 cagacgtccg gcgctgcaat tggtcgcgca aattcggcgg cgtgccttta atggtcaagg
    18001 tatcggttgc tggatcccag aagatgcgtt cacctaacag catcgcatca aagttaatag
    18061 tcaagccacc gccgctaccg gcaaacttcg tcaactgacg cagtgtgcta cggtctgccg
    18121 ggaagctctc ttccagttcg tagccttttt cagccgcaaa ctccgtgaag ctcacttcgc
    18181 ttacgcctgc cagctctttc gacagcgatt ccagttcgat ctcttcgcct gcctgcagct
    18241 gctcattaca gtagctataa acctgctggc gcacgttctg tcgctctgct ttatccagtt
    18301 gtgcttcggc agtgaaatca tcaaccgctt gcaacagccc acggttttgc gctttcgcgt
    18361 tcaatccttc gctggccccg aggaaatcca taaagaaatc cgccactttg cgccctactc
    18421 gccctttcag gaaagtgaga tagcgggtgg actctggatt ggtttcccat tcggttaaat
    18481 cgatacgcgc aacaatatcc gcatgattga tatcaagata atgggttggg ttgatgtcga
    18541 gattttcgtt aacgcgcata ctgctcaggt tgctcagaac tgccaccagc aaatactcca
    18601 ccgccagata acggtagtgg caaaacagca cgaatccacc gtcagcaaaa ggatatttcg
    18661 ctaattcgtc acgcaggcga ccggttgccg cgcggctaaa tgccagaaag tcctcttcgc
    18721 cctgacgctg taagcgcagc gtctgcgcca gttcactctc ttcgctaaac aagccgtaag
    18781 ctttattttt ggcgctatag acccgatgca gttctgccac catctcaacg acggtttctg
    18841 tcggttccag caatgaatcg cgcaacacca gctcaaggtt ctgctcatca cgcttgataa
    18901 gctggtgcag agcaatctgg ttgatatcca gactcatgat aaactctcct ttaagaccgg
    18961 gcggtattca accaccgcca gttaagcgac gcaataaaag atggcggtca ttttacagaa
    19021 ggcagcgtaa cgagaatgat ttaacgcata aaaaagcaga aaaaaaaccg ttgctacggt
    19081 aatatgttgc cctttcatca actaactgat ttcgatttat gccacaaatt tcccgctaca
    19141 gtaatgaaca ggttgaacaa ttgctcgcag agctgctcaa cgtactggaa aaacataagg
    19201 ctccgaccga cctttccctg atggtgttag gcaacatggt cactaacctt atcaacacca
    19261 gcattgcccc ggcccaacgc caggcaattg ccaactcttt tgccagcgcc ttacagtcct
    19321 ctatcaacga agacaaagcg cactaaggga aacagataac aggttatggt aactcatcgt
    19381 cagcgctacc gtgaaaaagt ctcccagatg gtcagttggg ggcactggtt tgcactgttc
    19441 aatattctgc tttcgctcgt cattggcagc cgttacctgt ttatcgccga ctggccgaca
    19501 acgcttgctg gtcgcattta ttcctacgta agcattatcg gccatttcag cttcctagtg
    19561 ttcgccacct acttgctgat cctcttcccg ctgaccttta tcgtcggctc ccagaggctg
    19621 atgaggtttt tgtccgtcat tctggcgacg gcgggaatga cgctattact gatcgatagc
    19681 gaagtcttta ctcgtttcca tctccatctt aatcccatcg tctggcagtt ggttatcaac
    19741 ccagacgaaa atgagatggc gcgcgactgg cagctgatgt tcatcagcgt gccggttatt
    19801 ttattgcttg aactggtgtt tgcgacgtgg agctggcaaa agctgcgcag cctgacgcgt
    19861 cgtcgacgct tcgcgcgccc gctggccgca ttcttattta tcgcctttat cgcctcgcat
    19921 gtggtgtata tctgggccga tgccaacttc tatcgcccga tcaccatgca gcgcgctaac
    19981 ctgccgcttt cgtacccgat gacggcgcga cgtttccttg agaagcatgg cctgcttgat
    20041 gcgcaggagt atcaacgccg tcttattgag caaggtaatc cagacgccgt ttccgtacag
    20101 tatccgttaa gcgaactgcg ctatcgcgat atgggcaccg ggcagaatgt gctgttgatt
    20161 actgtcgatg gcctgaacta ctcacgcttc gagaagcaga tgcctgcgct ggcaggtttt
    20221 gctgagcaaa atatttcgtt cacgcgccat atgagctccg gcaacactac agacaacggc
    20281 atctttggcc tgttctatgg catctcgccg agctatatgg acggcattct gtcgacccgt
    20341 acgcctgcgg cattaattac tgcgcttaat cagcaaggct atcagctggg gttattctca
    20401 tcagatggct ttaccagccc gctgtatcgc caggcattgt tgtcagattt ctcgatgccg
    20461 agcgtacgca cccaatctga cgatcagacc gccacgcagt ggatcaactg gctgggccgc
    20521 tacgcacaag aagataaccg ctggttctcg tgggtttctt tcaatggcac taacattgac
    20581 gacagcaatc agcaggcatt tgcacggaaa tatagccggg cggcaggcaa tgtcgatgac
    20641 cagatcaaac gcgtgctcaa tgcactgcgt gattctggca aactggacaa tacggtggtg
    20701 attatcactg ccggtcgggg tattccactg agcgaagagg aagaaacctt tgactggtcc
    20761 cacggtcatc tgcaggtgcc attagtgatt cactggccag gcacgccggc gcagcgtatt
    20821 aatgctctga ctgatcatac cgatctgatg acgacgctga tgcaacgcct gctgcatgtc
    20881 agcacacctg ccagcgaata ttcgcaaggt caggatttgt tcaaccctca acgccgtcat
    20941 tactgggtta ctgctgcgga taacgatacg ctggcaatta ccaccccgaa aaagacgctg
    21001 gtgctgaaca ataacggtaa ataccgcact tacaacttac gtggtgaaag agtgaaagat
    21061 gaaaaaccac agttaagttt gttattgcaa gtgctgacag acgagaagcg ttttatcgct
    21121 aactgattaa ttataaatca gttagcgaaa tatcttactt gcaatcggtg tggaaaacgg
    21181 tagtattagc agccacgagt cggcacgtag cgcagcctgg tagcgcaccg tcatggggtg
    21241 tcgggggtcg gaggttcaaa tcctctcgtg ccgaccaaaa atcccaagaa aaaaccaacc
    21301 cttacggttg gtttttttat atctgcaatt aattcgataa acagaccgtg acacatcaca
    21361 gcctgtttat tttctgttat cagaacgtcc agaccacacc cgcctgagta ttccacggcg
    21421 attctacccc tgcgccattg ccatagctga ctgacaaatg accgcttaac gtcggggtaa
    21481 acgatgacct tatcccagcc tgataaacgc cactggttcc agccacatca ttattaaatt
    21541 tgccatcgtc attaactttc acctggttag aatcggcgta ttcctgacgc acggcggctt
    21601 tcagccaggg ttccagcgtc gtaccgtttt gcaggtccat gtgatagctt accgccgttc
    21661 ccgcttcagc gcgtaatatc cgggtatttc ccacatccgc gcgcatgccg tttgataacg
    21721 tgtagtcctg accatctgtg gtaaagccgg taaaggccag atagggtcta acactccaca
    21781 atccgtcaac ccaacggaac ccgctctcaa catgagcacc cgcgccgtta ctattgtaat
    21841 cgccaaacgc tgttgcccca ttactcatct tgccatggat ggtgttggca aaacggtcaa
    21901 ctttcaccac cccatcaaca taggcaccgt tctgatgctc ccaaccggca taagccccca
    21961 gggtatagct atcgatatta ccttttccgc cgcgatcaaa accaatatca gaatgagagt
    22021 aaccaaagaa caagccgcga attgtactgc tttcttcacg ggagaaacgg ctatcgatac
    22081 cgagcgtcag gcccgtcaat gtttgctcaa aaccagctcc cgcatcagtg gtcacgttgt
    22141 tgcgggtgtt aattgccgaa ctccacatca ccgtatcgta actaacgcct tttacgctac
    22201 caagacgctc acgcacggtg tccagttctg catcaaatac cagcggttgt gcggctgcca
    22261 tgttcagcac atcagtggtt gaaggggtaa tttgcgcgcg attctctgcg aggctccagc
    22321 tatggttgcc attatccagc aaggtatatt catacgtacc gatatcaaca acgcctccgg
    22381 cattgcccaa cgtaaatgca gcatcaccgc cgcccgttgt taccagtgta aggctatctc
    22441 ctgctgccgg gctggcaccg gtgtccgtca cgaatatttt gaaatcacct gttgcctgac
    22501 cggtgacgtt gagctgatcg ctctgatgat tggccatatc ggtacgcata taaaaattgc
    22561 cgtttcccga cagggtattg gtggtcaacg taatgaaatt ttccgcactt gccgtcacag
    22621 atgaacgagt cactggctca gccatcataa tcacatttgc gccggacatc gcaaggttgg
    22681 ctaagttgag gcgagtgttt tggttcgtcg tagtgatatc ggcaatgatg gtccatgcgc
    22741 tattttcgag cgaaacgtcc gctttgctgg tatccacggc accatgcgtt ctaaaagaag
    22801 cgccctcaga aaccactacc ttacccacca acatagcggg tgcataattt atttgcggct
    22861 tcatgacctc aagaatgcca tcattccctc tgactgacac gttaaccatt gtgccagccc
    22921 agacattagc gcggccattg ttgattacca tattttcaga gatggctttt gaggaatacg
    22981 tgatacttcc attctggtct gatcgaccga gatcatatac cgcatttgcc tgcactttcg
    23041 taccagtatc ctttcccagc gattgcatag tggcatgctc atcaaggata gtgtcaatgg
    23101 cctgcgtgtc ctccataaca atgagcccgg aaccatcatc cagttcataa ttttttgaca
    23161 caccatcctt tatactaaat tggcctttac tgtttgtacc actcacttcc agcgcattcg
    23221 ttgagacaat taatttcccg cctgcttttt tatcaacgcc agttgcagtc ccgccatcca
    23281 taacctccag taagccgcca ctatccacag tagtattata agcgaagtca ttttcttcaa
    23341 ctcgcaaact tccgccgttt tccaacaaca tattattagc aataccattc ttgatatcga
    23401 actgaccgag acggtttgtt ccgaatacct ccatggcccg cgtggttgtt ttaatagcac
    23461 cgcctgtttt ctgatctacc gcaagcgcaa atcccccctc tttaacctcc agtagtcctc
    23521 ccttttcgat agcggcaaaa acggcccccc catctttttc aacgattaag cgcccgcctt
    23581 taatcgtggt atcattcgct gggccataaa cctgtagcac accattattg agggtagtct
    23641 ggtttgccgc gccacccatt tgaacaattt gataaccatc atttaatact gagtattctg
    23701 cggtaccatt catttcaatg tattgatacc ctccgttaat ttcagtatta ctacttacac
    23761 caaattcttt tatatgctgt tctccgccag gattgatttt cgtattattg gcggtcccat
    23821 aaaccagctg gatatctttt tccaggataa caccatcaac tgtctcacca tactcaactg
    23881 ttgatgccaa tgctatgggt gaatataacg ctgtagccac cagaacagat attgcggaac
    23941 gacaaagaga aacagaacca gattgatgca ttgagctttc atcctatgaa attaattgca
    24001 gttaaaagca ttgggtacag aaaataccca tagctccata cccggagtca gtttttaaaa
    24061 actgtttaaa gaaatgcaca agtattgtga ttgatttttt agttgttttt cttgatgaga
    24121 agctgatgca aaattccgtc tttataataa aaatgatgcc aaagcgaacg acaaggttgt
    24181 agttttcact acatgtccat acataaaatg gggtaacatt cacgcgcctg gtagcgttac
    24241 caacgctacg ctcaaacata atgattctaa taaaacctca ggagactact atgcctgaag
    24301 caacgccttt tcaggtgatg attgtggatg atcatccact tatgcgacgc ggtgttcgtc
    24361 agttactgga gcttgatcct ggctttgaag tggtcgccga agcgggcgaa ggcgcgagcg
    24421 cgatcgatct ggcgaataga ctggatatcg acgtgatctt gctggatctc aatatgaaag
    24481 gtatgagtgg cctggatacc ctcaatgcct tgcgcaggga tggcgttacc gcgcaaatta
    24541 ttatcctgac cgtatccgat gcctccagcg atgtctttgc gctgatagac gcaggcgcag
    24601 acggttatct gttgaaagac agcgacccgg aagtattgct ggaagcgatt cgtaccggag
    24661 cgaaaggcag caaagtcttt agcgaacgcg tcaatcagta cttacgtgaa cgtgaaatgt
    24721 ttggcgcgga agaagatccc ttcagcgtgc tgacggagcg cgagctggat gttctgcacg
    24781 agctggcaca ggggctgtca aataaacaga ttgcatcggt gttgaatatt tccgagcaga
    24841 cagtaaaagt acatattcgc aatctgctgc gtaaactcaa tgtccgctca cgcgtggcgg
    24901 ccaccattct gttcctgcaa caacgcgggg cacaataaaa atagcccgat ggatttatca
    24961 tcgggctgag atttatgaca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac
    25021 gtggatcgat caatttattg aatttacaca attttgtagg ccggataagg cgttcacgcc
    25081 gcatccggca taaactaagc gcactttgtc atcagtctag cccgatggca tcaccatcgg
    25141 gcctcttttt atttactctc ctgcggcgac aaatgttgca tcgcctgcgc gatactacgt
    25201 tcaatcaccg cacggcgagt atcgttggca ggtaagagtt tcaacatcat ctcccacgcg
    25261 gcaacggctt cgccaaatcg ctgctgctca aaggcattaa acgcatacat gcttagcaca
    25321 cggatattgc tatggtccgt tctcaccagc tgacgtagca gttcaccgcc gaggcggttg
    25381 tcgttgggat cagatgaacg agtcaacgct tcagcgtatc ccagtgcggc atcgctgttc
    25441 ttcggatcga ggcgatacgc agtagcgtat gcatcggtgg cgatactggc gttacccagc
    25501 gccatgccaa cgcggcccaa cataatccag ccttctatat ctcccggatt tttttgcagt
    25561 tgagtacgca tccccagcgc aaggcgcgac atctcttctt cgttgagcgg atcggctttc
    25621 gggtccagcg ccctgtccag caacgccgga gcctgtgccg tggcctgctg ccagattttc
    25681 acctgctgat aattgccagt ctggtagtag ctgacgccag ccacaattaa cgccaccaca
    25741 atacccggca gataaacaac atatccggca cgcttacctt ccggcacgct ttgttcagga
    25801 aacgcttccg gcaccacgcg tacccgacgc cgcgagcggg cgtatatgac ccaaccgcca
    25861 atgccaatag ccactactgg cagcacccac agcagcacgg tcagcggcgt taacggcgga
    25921 tcgtaagtga cgaagttgcc gtaacgcgcc accatataat cgacaatctc ttttttactt
    25981 ttaccttcct gcatcagttc atacactttc tgacgcaggt cggtggcaat catcgagttg
    26041 gaatcggcaa tgctgttgtt ctggcatttc gggcagcgca gttcttcagt gagctgacgg
    26101 aactgttgtt cctgtgcttc atctttaaac tgcaacacgt cgatggtcgc cagcgctgag
    26161 ccggagatca tcagcatcag cacgcccaat aaaaacctca ttgtgcggcc tccttactgt
    26221 atttctccca cagcggcttg atctcttctt cccagacgcg aggattgaga tcgcccgcat
    26281 ggcgatagcg aatgataccg ttgccgtcaa taaggaaggt ttctggcgcg ccatagacac
    26341 cgagatccag ccctaacatg ccgtcgccat caaacaggct tagcgcgtaa ggattgccca
    26401 gctctttcag ccagctgatt gccttctggc gatcgtcttt atagttcatg ccgaccacgc
    26461 gaatgccctg cgctgaaagc tgattcagat attgatgttc cgcacggcag gtcggacacc
    26521 aagtcgccca gacgttaagc agtactggtt tgccctgagt cagcacatcc gcctgataaa
    26581 actgccccgg attgtccagt gattcgagac gaaacttcgg cacaggcttg ccaatgagcg
    26641 ccgattccag attggtcgga tcatcccctt cggcattacg cgccagctgc cacagcagcg
    26701 ccgcggcaat cgccaggaag ataatcaacg gaattaacaa tactttgcgc ttcatacggc
    26761 ctccggcgca gttttttgcg gactcacgcg cttacgatag cgaggatcaa acagacacag
    26821 cagtccgccc aacgccatca tcagcccgcc cgcccaaatc cagcgaacaa atggtttgta
    26881 gtaaagacgc acggcccacg cgccgttttc cagctcttca ccgagcgcgg cgtacaggtc
    26941 acgcgtgatg ccgccgtcaa ttgccgcttc ggtcatcatc gacccggcag tgttgtaata
    27001 acgtttttcc gcatacagca ccgtttccgg tttaccctct cgcgttacgc caatagtcgc
    27061 cacaccgccg cgccagttcg ggccagtcac ctctttgaca tcacggaagg tgaagcgata
    27121 ttcatgaata tcgacgctat cgccggactt catgcgcaca tcacgctcaa cgctataatt
    27181 ctggctaaag gcaatgccaa caattgtcac tgccagcccc aggtgagccg ccaccatccc
    27241 ccaataactg aaggtggttt tcgtgccgcg tgaaatacgc agcgcagctt ccgcagttgc
    27301 cagcaccgca atccagcagg ccattgccag gccgagcacc gtcatcgcca caactttgct
    27361 ttcgaacagc cacggcaaca gcagagacag caccagcgta gagatgaagg cgataatcaa
    27421 taaattgcgg attttacgtg ggcgatcgcg cccccagcgc accagaggac cgacaccaag
    27481 cagtagcgca aacggcacca tcagccaggt aaacatggtg ttgaagaacg gttcgccaat
    27541 cgaaatactg cccagtccaa gttgcttgtg caccagcggc agcagcgtcc ccagcaacac
    27601 caccagcatc gcggcgacca gcaaaacgtt gttcgctaac agcaaagatt cccgcgacca
    27661 cagcgcattg tttacgcgtg agcgaacttt gtgtccacgc gcggcaaaca gcagcagcga
    27721 accgccaatc accagcacca taaaggcgag gataaacata ccgcgcgccg gatcagacgc
    27781 aaacgcgtgt accgatacca gcacgccgga acgcacgagg aaagtcccca acagacacaa
    27841 cgagaaggca ctgattgcca gcagtaatgt ccacgctttg aagctggcgc gttgttcagt
    27901 gaccgccagt gagtgcatca gtgcagtccc caccagccac ggcataaacg aggcgttttc
    27961 taccggatcc cagaaccacc agccacccca gccgagttcg taataggccc atgcggaacc
    28021 gagcacaatg ccgagcgtta ggaaaatcca cgccgccagc gtccacgggc gagtaaaacg
    28081 cgcataagtg ctgtccagac gcccgctcag caaagaagca atggcaaaag caaacgccac
    28141 cgagaaaccc acgtacccca tataaagcag aggcggatgg aagatcagcc ccggatcctg
    28201 taacagcggg ttaagatcgc gcccttcaat cgggaagttc ggcagcgtgc gtgagaacgg
    28261 gttagaggta aagagaatga acagcaaaaa gccgacgctg accatcccca ttatcgccag
    28321 tacacgggcc acaatatcca gcggaatacg ctgactaaaa atcgccaccg caaaggtcca
    28381 gccgctcatc agcagcaccc acagcagaag cgaaccttca tgcgcgcccc aagtagccgc
    28441 cacgcgatac cacaccggaa gctgggtatt ggagttgctg gcaacatagg tgacggtaaa
    28501 gtcgttgacc acgaaagcat tgaccagcac cagaaatgcg ccagccacag acataaacag
    28561 cagccaggca aacaagcggg aagacgccat catgcgcgca tctccgcgcg ccacgcccca
    28621 tagcggatac acggacagca gcagcgcaat ccctaacgcc aagcacagca gcccgtttcc
    28681 aatttccggc atcatgatgc tgggtcctta taaacactcg ccgggcgacg gtggttagct
    28741 tccatcgctt tctcaacttc tggcggcgtg tagttttcgt cgtgtttcgc cagcacttct
    28801 ttcgcgagga tatgattgcc tttttccagc tcgccctgca ccacaacgcc ctgcccttca
    28861 cggaacagat ccggcaaaat gccttcgtaa gagacatcca ctgagccttc agcatcgtaa
    28921 atggtgaagg tcactttcag cgaattggga tcgcgctgca cgctacccgg catcaccatc
    28981 ccgccaacgc gcagacgctg accgacttcc ggcatctgct gagtttcacg cttgccgtag
    29041 agaatttccc ccggcgtata aaagagatcg atattcgagc gcagcgcata tagcaccaga
    29101 ccgatagtca gcgctagccc tgccaacacg gcacaggcaa tccacaagcg gtttttacgg
    29161 cgaatattca tgcagcctcc tgctgttgcg cagcacgtaa acgcgcctca cgcgcccgct
    29221 gttgcgccac accacgcaga attgcgcgat gttgcatcac cgagtgcacg accaaaacca
    29281 ccagcggaat aacggtcatc accaccgcca gccagacaaa aaaggcgtaa ccgcccattg
    29341 cgaaaaattc attccaggaa gcaaatgcag gggtcattta cggcctcttt tcagtatcag
    29401 ttcactcacc cacggacggc gtttttccat cagcaaaatc aaattacgca tccgcatcag
    29461 cgtcagcgtg gcagacagga gcaggaagcc aaaaatcgac cagcgcagcg gcgaacgcat
    29521 cgccggatcg atactttgct gcatccgcgt tgatccctga tgcagggtgt tccaccactc
    29581 cacggagtaa tgaataatcg gcagattcac cacgccaatc agcaccatga tacctgccgc
    29641 acggcccgcc agacggcggt cgtcgaaggc gtgccacagg gcaatcacac ccacatacaa
    29701 aaacagcagc accagttcag aagtcagacg tgcatcccat acccaccagg tgccccacat
    29761 cggttttccc catgcagagc cggtaaccag ggcaataaag gtaaacacgg caccaatggg
    29821 ggccatcgcc gccaccgcca ggttggccat tttcatctgc cagacaaggc caataaacgc
    29881 cgccaccgcc attgatgcat aaatgcccat cgaccagatc gccgcaggca catgcaggta
    29941 gataatgcgg tagctatttc cctgctgata atcagccgga gcaaagccga atccccagat
    30001 ccagccgacg gtaagcacga ccacactggc aattgccagc cacggtataa accagccaca
    30061 gatttgatac agccgtggtg ggatcgccag ttgatgcagt gttttccaca tagtttcgat
    30121 accagactcg aacaaaaatc agtaatccag cgttattgaa tgctgattcg taacgctgcc
    30181 gccgtcgcaa aaggacttaa tgtcgcggtg cctgccagca acgcgcctaa aattgccaga
    30241 tacccatcaa cgggcaaatg catagaagcc gcgtccatcg cggcggtggc aaagatgagt
    30301 aatgggatag tcagcggtaa caccagtatg ctgagcagca caccaccgcg cttaagtccc
    30361 actgtcagcg ccacgcccgg tgcaccgaga aagccaagcg taggcgttcc cagcagcagc
    30421 gtcagcgcca tcacttgcca gccataaaca tccattccca gtagcattgc taccagtggc
    30481 gaaaggatga gtaacggcag accggttacc atccagtgcg ccatcacctt cgccagcaca
    30541 acggcgggca agggtaacgg caacaacatc aattgttcaa gactgccgtc ctgcaaatcg
    30601 tcacggaaca gtcgttccag cgccagcaag gatgaaagca gcgcagcaac ccagataatg
    30661 cccggtgcaa tacgcgccag cagttgcggc tccggaccga tactgagcgg aaaaagggta
    30721 attacaatca ggaagaacca cagcgggttg gcgatttcgg cgctatggcg aaacgctacg
    30781 cgcagttcaa gacggaaaat acgccagaac atcatgcggc cctcgtttgc gtcagtgaaa
    30841 tgcggcgaat tttactttca gcaacgttga gcggctggtg ggtagtcaga atcacaatcc
    30901 ccccctgctc cgtatgctgc gccatacgct gggtcagacg atcgacgccg ttaacgtcaa
    30961 tcgcggtaaa aggctcgtcg aggatccata atgtggcacg ggtcaaccac agacgcgcta
    31021 aagcgacgcg gcgttgttgc ccggcagaaa gttgatttac cggaatatct tcgaatccgg
    31081 caagcccggc ctgcgccaag gcttccagac attgtgcggc atcgccatcg cgatgataaa
    31141 agtgcagatt ttctaacgcc gtcagccggg ttttgatccc cggctgatgg cctatccata
    31201 acagggtttg atggtagctg tcgcgtacct gatgcaaggg ctgcccttgc cagagaacct
    31261 cgccagcgtc agggcgagac aaccccgtca gcaaacggag aagcgttgtc ttccccgcgc
    31321 cgttgctacc ggtgatttgt acccactctc ctgcgttcag cgtaaatgac aagccactaa
    31381 ataaggttcg ttcatcccgc tcacaaagta actctctggc ttcaagcata cccacgcaat
    31441 aaccctgtta aaaacctggc tcgacttcac gcatatcggg cagcttgtgc gcgatccctt
    31501 tatggcaatc aatacaggtt tgcccatctt tcaccgcctg gtcatgcatc ttcgcggcaa
    31561 ccgatttctg ggcggttgta tccatatact cgaagttgtg acagttacgg cactcctgcg
    31621 agttattgtc cttcatgcgc cgccactcat tctgtgccat cgtcagacga tgagcttcaa
    31681 atttctgcgg cgtgtcaata acgccaaaaa ttttaccata cagctcttta cttgctttga
    31741 gcttgcgtat catcttcggc acaaactcgt gcggaacgtg acaatccgga caggtcgcac
    31801 ggacgccgct acggttgttg tagtgcacgg aatccatgta ttcctgatac accgtgttgc
    31861 gcatttcgtg gcagctaatg cagaactctt cggtattggc tttttccatc ccggtgttaa
    31921 agccccccca gaagacgatg ccgccaacaa aaccgatcaa cagcagcgtc cccagcgcca
    31981 gatggctggg ggtacgccac catttccaca ggcgcttaat cagaccaggc ttacggtcag
    32041 aatttcccat aataacctct tatttcccgt aaccttttga tggggtaaag gtattcccca
    32101 cgattggcgc ggtatcggcc tgcggtacgt gacattgcag acagaaataa cgacgcggag
    32161 ccacttccgc gcccactttg ccgtcgctgt ccataaagtg agtaggactg atacgcggcg
    32221 cgccagtggt gcgatagctt tcgacaccgt ggcactgcaa gcagcgattg gtattggtcg
    32281 ttacctgata accttcaacg ctatgcggga tcattggcgg ctgattcaca tagttcagcg
    32341 gcatccgatc ctgctctttt ggcatccgaa tggccccttc ctgcgtcccg gatacttccg
    32401 gcgactggct aaaatcgacg ccattagccg cccaaaccgc gccgcttacc accagggcca
    32461 gcatcgccgt ccattgacac agcgctttct tcaggtcatg gcttttcatg atttcgctcc
    32521 cgaactccat cgtgtagtta ttgtaaatac atcctcagaa cagacatcca cgcagcgacc
    32581 gcacgtcatg caatcgcggc tggtgacctg caccgggctt tgttcatcca gcaccggggc
    32641 acgtagcaca tgcggttccg ggcaaacatg aaaacaatcc atacagcggt tacatttctg
    32701 ccgatcgctg gccgcaacgg taatcacacc tttgctaccc agcacgccat acagcgcgcc
    32761 aaccggacaa atgtgcccgc accagccgtg ttcaacgacc agtagatcaa ataaaaacag
    32821 cgcgagaata agcagcgcgc cgctgccgaa gcccattacc aggctacgcc ccatcagaga
    32881 aacggggttg atccattccc aaatgagcgt gccagttaag gctgatccca ccagaatgac
    32941 caccaacagc acgtagcgaa tgtggcgagg gatcgtcgca gactgattga ggtcaaagcg
    33001 cctgcgtaac cagttcgcta agtcggtaat cgggttcagc gggcagaccc agctgcaaaa
    33061 taatcgcttt cccgccaggg cgtagagcac ggtgataatc accgcaccgg tcaacgccac
    33121 cgtggcaggc agatgaccac tggcaagact ttgcagcgtc atcagcggat cggtcagcgg
    33181 aacggtgtcg aataacaggc tactgctgta gttgccgtgc aagatccaca caccaaacca
    33241 tggaccgctc aaaaacatcc ccagcacgaa gaactgacaa aggcgacgta acaccagcca
    33301 acggtgactg cgccaccagc cttttttctc cagcgcctcg cgcccggcgt cacgtttacg
    33361 atttgccatt gttcccctcc agccagccga agcggtaatg gtgacctaac tcccctttcg
    33421 ccagtgacag cggtaacacc ttgattgccg gttgttccag cacgcatact ttttcgcact
    33481 taccgcaacc ggtacaggcg tcgctgtgaa ccgtcggcag aaagcgggcg tgcttaccgg
    33541 tacgcgtgtt gcgctccagc tccaaggtga tggcctcatc aattttcggg cattcgcgat
    33601 aacaaacatc gcagcgcagc ccctgaaagt tgagacagtt ttcctggtcc accagtaccg
    33661 ccagccccat ccgcgcgtcg tcgatcgatt caatctcacg atccagcgca ccgcttgggc
    33721 acactttggc gcacggaatg tcctcacaca tttcgcaagg aatatcccgt gcgacaaaat
    33781 atggcgtgcc cgccgacaga ccagaggcca gcgtcgccag ttttaaggtg tcgtaagggc
    33841 aagcctgaac acactgaccg caacgcacac aggcactggc aaaggcgttc tcgtttatgg
    33901 ctccgggcgg gcgcaaccgc acgccagatg cgcgtgcggt ttgctgttgt aaccccagcg
    33961 ccacacccac ggcagccagc ccgcctgctg tgcgaacgac atcgcgcaga aagcggcggc
    34021 gaccattttg aggtttcgct gaccgggaca taatggctta caccttctcc agtttgaccg
    34081 cgcacttctt gaagtccgtc tctttcgaga gcggatcggt cgcatccagc gtcagtttgt
    34141 taaccagctg tgcggcgtcg aagaacggca tgtacaccag gccctgtggc ggacggttac
    34201 gaccgcgcgt ttcaacaatc gagatcactt cgccacggcg agaaaccact ttcactttgt
    34261 cgccacggcg cagatcgcgc gctttcgcat ccagcgggtg aataaacaag accgcttccg
    34321 ggaaggcgcg gtgcagttcc ggcacacggc gagtcatact gccggtgtgc cagtgctcca
    34381 gaacgcgtcc ggtagagagc cacaggtcgt actcttcatc cggtgcttcc gccgccggtt
    34441 cgaacggcag tgcgaagatc accgctttgc catccggttt accgtagaac ttatagcctt
    34501 cgcccgcttt cacgtacggg tcgttacctt cgctgtaacg ccactgtgtt tctttaccat
    34561 tcaccaccgg ccagcgcaga ccgcgcgctt tgtggtagtc atcgaacggt gccagatcgt
    34621 gaccgtgacc gcgaccaaac catgcgtact cttcgaacag ccctttttgc agatagaagc
    34681 ccagctcgcg ggattcatcg ttcagctgat cttccgccag ttcggatacc gggaatttgc
    34741 tcacttcggg ggtggcatac agaacttcgt acagcgtttt gccacgcagt tccggtttct
    34801 tcgccagcag ctcttccggc catacttctt cagttttgaa gcggcgggag aactggacta
    34861 actgccagag atccgatttc gcttcgcctg gcgcctgtac ctgctgacgc cagaactgag
    34921 tacggcgttc ggcgttaccg taagcgccct ctttctctac ccacattgcg gtcggcagga
    34981 tcaagtcggc ggccagcgca ctgactgtcg gatacggatc ggagacgatg atgaagttgc
    35041 gcggatcgcg ccagcccggc atacgctctt cattgatgtt cggcccggcc tgcatgttgt
    35101 tggtacacat ggtccagtaa acattcagct tgccgtcttt cagcgcacgg tcttgtgcca
    35161 ccgcatgaag accgattttc gccggaatgg tgccgctcgg gatattccac ttcttctcac
    35221 agatatcacg atgtttctcg ttagttacca ccatgtccgc aggcagacgg tgagcaaagg
    35281 tgcccacttc acgcgcagta ccacacgcag aaggttgccc ggtcagggag aacggaccgc
    35341 aacccggctg ggaaattttg ccggtcagca ggtgcaggtt gtagaccagg ttgttagccc
    35401 acacaccacg ggtatgctgg ttgaagccca tcgtccagta ggagatgact ttcttgttcg
    35461 gatcggcata cagctgcgcc agttgttcta actggtcttt cggcacgccg gtcatttcag
    35521 cggttttttc cagcgtatac tcagcaacga aggctttgta atcttcaaag ctcatcggtt
    35581 cggaggcgtc agaacccgga ttcttcgctg ctttttccag cggatgggtc ggacgtaaac
    35641 cgtagccgat gtccgtcgcc cctttgcgca ggttaacgtg cttgctgaag aagtcctgat
    35701 ttatcgcatt gttttgaatg atatagttgg cgatgtagtt caggatcacc aggtcagatt
    35761 gcggcgtaaa gatgatgccg ttatccgcca gctcgaagct acgatgctgg taggtagaaa
    35821 gcaccgccac ggtgacgttc tggttagaga gacgactgtt agtgatgcgt gaccagagga
    35881 tcgggtgcat ctccgccatg ttagcgcccc acagcacaaa cgcgtcagcc tgctcgatgt
    35941 cgtcatagca gcccatcggc tcatccatac caaaggtacg cataaagcca actactgccg
    36001 acgccataca gtgacgtgcg ttcgggtcga tgttgttcga acggaagccc gctttgaaca
    36061 gcttggacgc ggcataacct tcccagatag tccactgacc agaaccgaac ataccgatcg
    36121 attccggccc tttttctttc agggcggttt tgaatttctc ttccatcaca tcgaaggcct
    36181 gatcccaggt gattggggta aattcgcctt ctttgtcata tttaccgttt ttcatacgca
    36241 gcagcggctg cgtcaaacgg tctttaccgt acatgatctt gggcaggaaa tagcccttaa
    36301 tgcagttcag gccacggtta accggtgcgt ccgggtcgcc ctgacaggcc accacacgcc
    36361 cctgctgcgt tccgaccaga acgccgcaac cagtaccgca gaaacggcac ggcgctttat
    36421 cccatttaat ggcttcctgc tgaccaacaa cggcgcgggc aacgcccggc acgctgagac
    36481 cggcagccgc cgcagcggcc gcaacggcgt tagctttcat aaagctacga cgactgagtt
    36541 tcatggtgtt tcctcacctt gctcttcctg ctggtgataa accagcgaca ccgccagcac
    36601 gccctctacg ttgcgtactg actcaatggt ttggatcagc gtttcgctgt cttctgcttc
    36661 caccaccaca atcaactgac cgctcggcgc gtcgctgaca gcaacttcac agccgggaaa
    36721 ggcgttcagt tgggtgctga tgtctgaaat tcgttcgctt ttggcctgca cgaccaggct
    36781 gcaaacttgc cagttagtgt gcatggagat actccgcagt tatggctgat accggacagc
    36841 tggctgcaca tgctccacat ccgttgcaaa gttggctatt aagttgcgcc tggtagatcc
    36901 cggagagcgt cgggcgaaag ataattgcca ttggctcaca gctatcctga cagcggcggc
    36961 attcaactga ctgatacgcc aggcacgcgt ccccgatggt gaactgcaaa tcccaggccc
    37021 tggtgtggcg cggagaaaat agcgattcgg ggcaagcctg cgcgcaggcg taacagaagc
    37081 tgcactcgtt atttttgaaa ttaacgctcg gatagccacc cgtgccgcgt tgcagaatgt
    37141 tgttttcaca agcattaata caggcgtcgc agcgggtgca atgggtcaga aaatgagatt
    37201 catcaccgga ccagggcgga cggataccgt tactggcttt gcgccagcaa ccagtgagta
    37261 tgccccgacg ggatgcatca atcttcacat tgaccttcca tcattaacgc gctctgaaaa
    37321 ttgagagcga ccaaatagac cgcataatta ataagccatt tttatagccg ctaagatatt
    37381 aaaggatgtg tcaaagatgc ataccccgat cggggtaaaa cctgtagcag gatcaaaaga
    37441 gtggcgggaa gcgtggcaaa aacgggcttt tgctcacatt tcaaatggtt ataaacatat
    37501 ttatatagcg attaattcac cagagatatt tctgctggtt tgctccctca tcagaattta
    37561 acactaaaag a
//
LOCUS       DAZOYF010000019        36704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZOYF010000019 DAZOYF010000000
VERSION     DAZOYF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.019"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            16..372
                     /locus_tag="ACHUOF_000959"
     CDS             16..372
                     /locus_tag="ACHUOF_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR0610244.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            376..1503
                     /gene="dapE"
                     /locus_tag="ACHUOF_000960"
     CDS             376..1503
                     /gene="dapE"
                     /locus_tag="ACHUOF_000960"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR0610245.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            1531..1731
                     /locus_tag="ACHUOF_000961"
     CDS             1531..1731
                     /locus_tag="ACHUOF_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR0610246.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1841..2539)
                     /gene="ypfH"
                     /locus_tag="ACHUOF_000962"
     CDS             complement(1841..2539)
                     /gene="ypfH"
                     /locus_tag="ACHUOF_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0610247.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(2613..4628)
                     /gene="tmcA"
                     /locus_tag="ACHUOF_000963"
     CDS             complement(2613..4628)
                     /gene="tmcA"
                     /locus_tag="ACHUOF_000963"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR0610248.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(4643..5506)
                     /locus_tag="ACHUOF_000964"
     CDS             complement(4643..5506)
                     /locus_tag="ACHUOF_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR0610249.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(5674..6387)
                     /gene="purC"
                     /locus_tag="ACHUOF_000965"
     CDS             complement(5674..6387)
                     /gene="purC"
                     /locus_tag="ACHUOF_000965"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR0610250.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(6600..7634)
                     /gene="bamC"
                     /locus_tag="ACHUOF_000966"
     CDS             complement(6600..7634)
                     /gene="bamC"
                     /locus_tag="ACHUOF_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR0610251.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(7651..8529)
                     /gene="dapA"
                     /locus_tag="ACHUOF_000967"
     CDS             complement(7651..8529)
                     /gene="dapA"
                     /locus_tag="ACHUOF_000967"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR0610252.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            8675..9247
                     /locus_tag="ACHUOF_000968"
     CDS             8675..9247
                     /locus_tag="ACHUOF_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR0610253.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            9247..9717
                     /gene="bcp"
                     /locus_tag="ACHUOF_000969"
     CDS             9247..9717
                     /gene="bcp"
                     /locus_tag="ACHUOF_000969"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR0610254.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            9970..10587
                     /gene="hyfA"
                     /locus_tag="ACHUOF_000970"
     CDS             9970..10587
                     /gene="hyfA"
                     /locus_tag="ACHUOF_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR0610255.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            10587..12604
                     /gene="hyfB"
                     /locus_tag="ACHUOF_000971"
                     /pseudo
     CDS             10587..12604
                     /gene="hyfB"
                     /locus_tag="ACHUOF_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            12615..13562
                     /gene="hyfC"
                     /locus_tag="ACHUOF_000972"
     CDS             12615..13562
                     /gene="hyfC"
                     /locus_tag="ACHUOF_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR0610256.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            13579..15018
                     /gene="hyfD"
                     /locus_tag="ACHUOF_000973"
     CDS             13579..15018
                     /gene="hyfD"
                     /locus_tag="ACHUOF_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFR0610257.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            15030..15680
                     /gene="hyfE"
                     /locus_tag="ACHUOF_000974"
     CDS             15030..15680
                     /gene="hyfE"
                     /locus_tag="ACHUOF_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR0610258.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            15685..17277
                     /gene="hyfF"
                     /locus_tag="ACHUOF_000975"
     CDS             15685..17277
                     /gene="hyfF"
                     /locus_tag="ACHUOF_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR0610259.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMA
                     KPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQ
                     RSTWHDFLPSGTASVSEKHSER"
     gene            17267..18981
                     /gene="hyfG"
                     /locus_tag="ACHUOF_000976"
                     /pseudo
     CDS             17267..18981
                     /gene="hyfG"
                     /locus_tag="ACHUOF_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            18991..19536
                     /gene="hyfH"
                     /locus_tag="ACHUOF_000977"
     CDS             18991..19536
                     /gene="hyfH"
                     /locus_tag="ACHUOF_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR0610260.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            19533..20291
                     /gene="hyfI"
                     /locus_tag="ACHUOF_000978"
     CDS             19533..20291
                     /gene="hyfI"
                     /locus_tag="ACHUOF_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR0610261.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            20284..20697
                     /gene="hyfJ"
                     /locus_tag="ACHUOF_000979"
     CDS             20284..20697
                     /gene="hyfJ"
                     /locus_tag="ACHUOF_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR0610262.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            20727..22739
                     /gene="hyfR"
                     /locus_tag="ACHUOF_000980"
                     /pseudo
     CDS             20727..22739
                     /gene="hyfR"
                     /locus_tag="ACHUOF_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            22761..23633
                     /gene="focB"
                     /locus_tag="ACHUOF_000981"
                     /pseudo
     CDS             22761..23633
                     /gene="focB"
                     /locus_tag="ACHUOF_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(23727..24788)
                     /locus_tag="ACHUOF_000982"
     CDS             complement(23727..24788)
                     /locus_tag="ACHUOF_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0610263.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            25001..26464
                     /gene="bepA"
                     /locus_tag="ACHUOF_000983"
     CDS             25001..26464
                     /gene="bepA"
                     /locus_tag="ACHUOF_000983"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR0610264.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            26485..26844
                     /gene="arsC"
                     /locus_tag="ACHUOF_000984"
     CDS             26485..26844
                     /gene="arsC"
                     /locus_tag="ACHUOF_000984"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR0610265.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(26977..27678)
                     /locus_tag="ACHUOF_000985"
     CDS             complement(26977..27678)
                     /locus_tag="ACHUOF_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR0610266.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(27773..29062)
                     /gene="uraA"
                     /locus_tag="ACHUOF_000986"
     CDS             complement(27773..29062)
                     /gene="uraA"
                     /locus_tag="ACHUOF_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR0610267.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(29148..29774)
                     /gene="upp"
                     /locus_tag="ACHUOF_000987"
     CDS             complement(29148..29774)
                     /gene="upp"
                     /locus_tag="ACHUOF_000987"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR0610268.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            30098..31135
                     /gene="purM"
                     /locus_tag="ACHUOF_000988"
     CDS             30098..31135
                     /gene="purM"
                     /locus_tag="ACHUOF_000988"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR0610269.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            31135..31773
                     /gene="purN"
                     /locus_tag="ACHUOF_000989"
     CDS             31135..31773
                     /gene="purN"
                     /locus_tag="ACHUOF_000989"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR0610270.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            31945..34011
                     /gene="ppk1"
                     /locus_tag="ACHUOF_000990"
     CDS             31945..34011
                     /gene="ppk1"
                     /locus_tag="ACHUOF_000990"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR0610271.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            34016..35557
                     /gene="ppx"
                     /locus_tag="ACHUOF_000991"
     CDS             34016..35557
                     /gene="ppx"
                     /locus_tag="ACHUOF_000991"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR0610272.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(35596..>36704)
                     /gene="pdeF"
                     /locus_tag="ACHUOF_000992"
     CDS             complement(35596..>36704)
                     /gene="pdeF"
                     /locus_tag="ACHUOF_000992"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR0610273.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         8495 a         9711 c         9552 g         8946 t
ORIGIN      
        1 gaaaaaggat tcattatggt tacactttac ggcatcaaaa attgtgacac cattaaaaag
       61 gctcgccgtt ggctggaagc caataacatc gactatcgtt ttcatgatta ccgcgtcgat
      121 gggctggaca gcgaattatt gaacggtttt atcaacgaat taggctggga agcgttactc
      181 aacacccgtg gtacaacctg gcgtaaactg gacgaaacta cccgcaataa aatcaccgat
      241 gcggcctctg cggcggcatt aatgactgaa atgcctgcaa ttatcaaacg tccattgctc
      301 tgcgcgcctg gtaagcctat gctgctgggt ttcagtgatt ccagttatca gcaatttttc
      361 catgaggtgt agtctatgtc gtgcccggtt attgagctga cacaacagct tattcgccgc
      421 ccttccctga gtcctgatga cgcaggatgt caggctttgt tgattgaacg tttgcaggcg
      481 atcggtttta ccgttgaacg catggatttt gccgatacgc agaatttttg ggcatggcgt
      541 gggcaaggtg aaacgttagc ctttgccggg cataccgacg tggtgccgcc tggcgacgcc
      601 gatcgttgga tcaatccgcc gtttgaaccc accattcgtg acggcatgtt attcgggcgc
      661 ggtgcggcag atatgaaagg ctcgctggcg gcgatggtgg tagcagcaga acgttttgtc
      721 gcacaacatc ccaaccatac ggggcgactg gcatttctga tcacttctga tgaagaagcc
      781 agtgcccaca acggtacggt aaaagtcgtc gaggcgttaa tggcacgtaa tgagcgtctc
      841 gattactgcc tggtcggcga accgtcgagt atcgaagtgg taggtgatgt ggtgaaaaat
      901 ggtcgtcgcg gatcgttaac ctgcaacctc accattcatg gcgttcaggg gcatgttgcc
      961 tacccacatc tggctgacaa tccggtacat cgcgcagcac ctttccttaa tgaattagtg
     1021 gctattgagt gggatcaggg caatgaattc ttcccggcga ccagtatgca gattgccaat
     1081 attcaggcgg gaacgggcag taacaatgtt attccgggtg aactgtttgt gcagtttaac
     1141 ttccgcttca gcaccgaact gactgatgag atgatcaaag cgcaggtact tgccctgctt
     1201 gaaaaacatc aactgcgcta tacggtggat tggtggcttt ccgggcagcc atttttgacc
     1261 gcgcgcggta aactggtgga tgcggtcgtt aacgcggttg agcactataa tgaaattaaa
     1321 ccgcagctac tgaccacagg cggaacgtcc gacgggcgct ttattgctcg catgggggcg
     1381 caggtggtgg aactcgggcc ggtcaatgcc actattcata aaattaatga atgtgtgaac
     1441 gctgccgacc tgcagctact tgcccgtatg tatcaacgta tcatggaaca gctcgtcgcg
     1501 tgatgagtgg ttctgcaaga ggaaataagc atggactggc tggctaaata ttggtggatt
     1561 ctggtgattg tctttttggt aggcgtcctg ctgaacgtga ttaaagatct caagcgcgtc
     1621 gaccataaga aatttctcgc caacaagccg gagcttcccc cgcatcgtga tttcaacgat
     1681 aagtgggacg atgacgacga ctggccgaaa aaggatcaac cgaagaagta agctgagtgc
     1741 tcaaccgtag gccggataag gcgttaacgc cgcatccggc aatggtgaac gatccctgat
     1801 gcgacgctca cgcgtcttat caggccaggt aatctgtctt ttacatcatt tcaatcacat
     1861 catcatcgcc aggcttaccg ccacttagcg cttggtcgaa ataatgtttc ggaatggtat
     1921 aacgcaagtg atccagggca aattgcatac tgcgattgtc gatcgcatgg cctaaatcct
     1981 ccacgatatc cagcgtcacg tcaccaccgg cactgattaa cgcttcctgc gccgcaactg
     2041 catgcgccag atcgataacc ggatcttcac caccatgaat caagtgaatt gtcgtggctg
     2101 tagacgcggt ctccggcagg ctcgcatagc gtccgttaaa ggcaatgacg cgcgaagcaa
     2161 ggcccggttc ggctttaatg ctctccagcg ccataatcgc gccttgtgag aaaccaatta
     2221 gcgccgtggc gttagcccct actccgctct gtttctgcca gtagcgtacc gtttcaataa
     2281 acgtcggcat gatggcatcc acacgcgcct ggcgattatc ttccgtaata ccctgcactg
     2341 aaaaccactg acgccccgcc ggattaccgc tcggctccgc gccaccgaca ctcaccacca
     2401 gcgcatcagg aaacagcggt gcaaaccagc tgcctatttc ccccattgcc accgggttat
     2461 ccccgacacc gtggaaaagc agtaacaact gttgtgcagg tttatccggg ctttgaacaa
     2521 caaaatggtc atgtttcatg gcgaactcct taactgatgt cattaatttt acgccgctga
     2581 ctcacaatga ccatgggaga aaattgaagg agtcagtgaa aaaattgcca ttgcgttatg
     2641 cgatcgcgca gacgctcagt gcgaacctca tttagtgcga acagcgcctg cgcggcctct
     2701 tcccgctggc gaaccagtaa catcttgcgg cctgaaagtt taagtgtggt acataactgc
     2761 gcgtcactgg cgtttttctg taaacgccca cgcagcgccg gtaatgccag ttcactggtt
     2821 tgtagcagac gcaataagca acctaacgat gttaatagcg gacgatgagc gaaagcaaaa
     2881 ccggccagtt caagccagtc gtcgtcagaa aggatggcat cgtttagtgg atcaacagga
     2941 agcatttcgc cattccactg cgcgagagct tgcgcatcgc gacgtaaacg gtaatgctca
     3001 cgttcagcca gctgtttacc cgcatcactc atcggtaaca gcgccatcgc cgtatagcaa
     3061 ccgctgctgg cttcccgatg attacccatc cgcaccagca caaaaccgca gcgttgccag
     3121 aaacgccata actccccagt gtaaccaaaa ctcaccgaaa gatagtcgag gtcatgcgta
     3181 tattgcaaag ctccagcaat aagttgtcgc cctgtgcctt cccgctgacg agccggatga
     3241 actgctatcc ggctaacccg ccttccgcgc aatgtcgccg ccagtggatt gctgccgtgc
     3301 gccgccagcg actgggccac cagattaccc cgcgggcgac gaaaacctgc ccataccgcc
     3361 tgactgagtt cttgagataa tccgccctca tccaccagcc acaacgcccc ggcaatctcg
     3421 ttttcgccag ccgcctgtaa aaaatgttgc cctggtgcat ccatcatccg gcgtaaatcc
     3481 agcggcgagg tccggtagtg cgcaccagac aataactgat aaacctttaa cggcgtttct
     3541 ggctcgcttc gccataacgt ttgttcaaat gcggaaatga cgatattgcc ttgtggtgta
     3601 tgggtgaagt tttcatcgtt aaaaaccagt gcctcactaa ccattttttc cagcgggcat
     3661 ccctgtgccc agcggatcgg ctgttgcagt tcaaaacggt gtaaatgcgg aaagcgagcg
     3721 caaaatttca gcaaaaaacc acgtccggta ccttcgtagc cctgcaccgt agtggttaac
     3781 aacgttcgag gaaaacgcga taccagttga tgcaacaacg gcgcaggtat ggctgcggct
     3841 tcgtcgacca ccagccagtc ggcttgctca tcgctggcta acaaggcatc cggtgcaata
     3901 aagcgaaact tctcgcccgc aaattgtgcc agtacatccg ttgccgcttt tgcgggcgcg
     3961 gtgacaatcg cactgcccgc aatacgagaa atgagttgcc ctgccaacgc cgacttaccg
     4021 cgcccacgcg cagccgttac cgctgccacg cccggcggca tggtcagtag ctgctgtaag
     4081 agttgctgtt gttctggttg tggtgcgccg gtcgcggggt gccagtcagt acggggagta
     4141 aaatgcgcca acgagaacgg ctggttttgc cgccagagga tagcgtcgtt atccgccgta
     4201 agtacgcgtt tgagatgctg gacaaaatgc ggcgtcgcaa tagggtcagg gcaatcactc
     4261 cagcgcagcg agtcggcatc aggttggttt tcccactctt cccatacagg gagtaacaaa
     4321 accagccagc ttcccgcttt caacgttccg ctaagtgctg caaaggcagc ggcatcaaag
     4381 ccgtggcggg cgtcgaatac cgcatgccgg aactcgcgcc caagtaaagt ttgtagcgcc
     4441 gagggtgaac agtggttttc agcatctagc tgcggcgaaa tccacagcca gtcgccaggt
     4501 aaggcatcac gcaacttaag agcatgatca aaacaccaac cctcttcccc gctcaacacc
     4561 agcaagcggc agatcccttc acgtttcatt tgcgctgtta atgtgtaaag cgcagtcagt
     4621 tcagccatcc ctgccccgga aattaaatgc ttttaccaaa agtattgcat tgtgccggat
     4681 cgccgctgtc gaaaccacgt ttaaaccagc tgtagcgttg ctgagaagtg ccatgtgtga
     4741 aactgtctgg tactactcgc ccctgacttt gctgttgtaa acggtcatcg ccgatggcct
     4801 gcgccgcatt cagcgcctct tccagatcgc cagtttccag aacgccttgc tgctgcatac
     4861 tatgccccca gacaccggca aaacagtcgg cctggagttc catacgcaca gataagcggt
     4921 ttacttccgc ctgcgtcgcg ttttgttgca gttgacgaac tttcggctcg atgcctaaca
     4981 gtttctgcac atgatgaccg acttcatggg cgataacgta cccctgggca aaatcgccat
     5041 ccgcgccaag tttgtctttc atgtcatcat agaaggagag atcgatataa actgtgccat
     5101 ccgccgggca atagaacggc cccataatgg actggcccgc cccgcagccg gtgcgcgtca
     5161 ttccacggta catgaccagc ttcggttgct gataggtctt acccatcttt tcgaactgtt
     5221 gtccccaggt gtcttccgtg gttgcctgaa tcaccgaggt gaattttgcg gcttcgtctt
     5281 cattagggct aattgaccgc gttgattgtt gttgagaaac cggctgcccg gtcatcaacc
     5341 cggttaaatc aacgccatag tagcctgcaa ccagcacgac tatcagtaaa atcagcccgc
     5401 ctttaccgct cggcagacgg aaaccgggac cgcccattga cggaccgcca gagctgttgc
     5461 gcctgtcttc aacattgtca ctttcacgtc gcccttgcca acgcatagat acctcaacaa
     5521 tatattcatt atagaaatga tcgtaggtgg ttcagaggaa gattaccaca ggaaagcggg
     5581 atgaaatggc ggaggaatga gcgttcaatg catatattcc aagagcggtt gtcttgccgt
     5641 gccagctgca cggcaagatg atgaacagaa aaatcagtcc agctgtacac ccaggcggcg
     5701 ggcgacggct tcataggctt cgatcaggcc accgaggctc tggcggaaac ggtctttgtc
     5761 cattttctcc agtgtttctt tgtcccacag gcggctgccg tccggggaga actcatcgcc
     5821 cagtaccact tcacctttgt acagaccaaa ctccagcttg aagtcgacca gaatcagacc
     5881 agcatcatcg aacagttttt tcagcacgtc gttcgctttg taggtcagct ctttcatacg
     5941 cgccaggttc tctttgctca cccagccaaa ggtttcgcag taagattcgt tgaccatcgg
     6001 atcgtgcatg gcatcgtttt tcaggaacag atcgaacagc ggcgggttca gctcaatacc
     6061 ttcttcgatt ccaagacgtt tcactagaga gccagcagca cggttacgca cgacacactc
     6121 aaccggcacc atatccagct ttttcaccag acattcggta tcggagagca gacgctccat
     6181 ttgagtcggg atacccgctt cagccagttt gctcataatg aagtagttga acttgttgtt
     6241 caccatacct ttgcgatcaa actgctcaat gcgcgcgcca tcccctgctg acgtatcatt
     6301 gcggaattcg agcaccaaca ggtccgggtt ttccgtgctg tatacggttt tcgctttacc
     6361 acgatacaac tcagcttgct tttgcatctt tatcactcct gggtgtgaat taacgtttta
     6421 aaatcttttg ctgtctggtg tgccggatgt tttgtcggat gcggcgtgaa cgccttatcc
     6481 gaactacgtc cgacaaacaa atttcgtgcg aattaccgct aaaatcgcgt tttcctgccg
     6541 acgcacacgt ttgcgtatca tatcagaaaa aagggccgga tgattccagc cctgtatttt
     6601 tacttgctaa acgcagcctg gaagacagct accagcgcgt cgttctgact ctgagtcaga
     6661 gtatgacctt tcggatcgat gaactgtagg ctgctgcggt tatctaaatc gccaacctgc
     6721 agtttatagt caccggatgc caggcctgga tcgctcgcgc ccagttcctg ccagtcgctg
     6781 tcagacagcg gcttataagt tacggccatg ttgccctgcg aacgggtgct gtcggtcact
     6841 ttcatgccca ctttttccag cgccgctggc agacgttgcc aaaccacatt gaacggcccg
     6901 cgtacgacca gcattggtaa accggtgtca tcagctgcac tttgtacgtc catagtggtg
     6961 gaggcacgat tttgcgcagc gttcgcggcg tcagtggcag atttatccag accggcggaa
     7021 ataacgttca tcatctccgt gctgtaacgc tgcatggaag ccgcgtctgc aaccggtttg
     7081 cccgcctgtt ccaggttcag cagtttaacc gtaaccgcct gctgataacc ctgcggctta
     7141 acagagattt gataacgacc acgatactgc tcgtcttcgt ccagacggtt ccattgtacc
     7201 caatcggtgg tcagtgtctg accagcatca tcacgttggg tgatggtgta gtttttcgcc
     7261 tgcagcacgc taaccacctg cggccacaga gtattgccac gaccattttc caccagcaat
     7321 gaagcggtat cgcccgtgaa ctgggtacgc gcgccagaaa ccagtgccaa cggctgggct
     7381 ggtggacgaa tgtccagcgc cttaccgaca gcaccactac cgttggtcac cgggattgca
     7441 taatcaccgg aggtcaccgg caaaatcatt ccagccgggg catgaagctc cgcaagcggt
     7501 gccgcttcca ggtaggcttc atcaccactg acctgacgct tatagcgtga gtcagaacta
     7561 caggcagcga gtaataaaac aagcgaaaca cccgcaacct ttgccaggcg cgacttttga
     7621 acagagtaag ccatcaaatc tccctaaact ttacagcaaa ccggcatgct taagcgccgc
     7681 tctgaccgtc tcacgaccac tgtcggtgat tggtgtcatt ggcaggcgca gcgtatcggt
     7741 cgccacaaga cccagttcct tacatgccca tttcaccggg attggattgg gttcgacaaa
     7801 tagtttgttg tgtaatggca tcagacgctg attaataacg cgtgcctcgg caaaatgccc
     7861 ttctgctgcc agtttgcaca tctgggccat atcacgcgct gcgacgttag ccgtaacgga
     7921 aataactcca tgaccgccca attgcatgaa gtccagcgcg ctcgcatcat cgccgctcag
     7981 cagaacaaaa tcatctgaaa ccagctcttt gatctggttt acacgcgtta agttccctgt
     8041 tgcctctttg attccgataa tattttttac tttcgccaga cggcccaccg tttccgggag
     8101 cagatcgcag ccagtacggg acggcacatt atacagaatt tgcggcaggt cagtatgctc
     8161 agcgatggct ttgaaatgct gatacaaacc ttcttgcgac ggacgattgt agtaaggggt
     8221 taccgtcagg cagccgacga taccactgtc attgaagcgc tgcgtcaggc taatggcttc
     8281 cgcagtagcg ttagcgccgg tcccggcaat taccggaatg cgcccatcag ccagatccag
     8341 cgtcatcatc accacatcag catgttcgtc atgatttaag gtagcggact cgccagtggt
     8401 gccaacagaa acgatcgccg aagtaccgct ggcaacatga taatcaatca gttttttcaa
     8461 gctagcccga cagacattac ctttttcatc catcggagta acaatcgcga caatacttcc
     8521 cgtgaacatg ggccatcctc tgtgcaaaca agtgtctcaa tggtacgttt ggtatggcat
     8581 taaaagcaag cagacagaac cgttctgatt gttgtatgca tgattttttt atgctttcct
     8641 taagaacaac tcacccctta aaggaataac cagtttgaca ctgtcatcgc aacattatct
     8701 ggtgatcact gcgttgggtg ccgatcgccc tggaattgtg aacaccatca cccgtcatgt
     8761 cagtagttgc ggctgtaata ttgaagacag tcgcctggcg atgctgggag aagagttcac
     8821 gtttatcatg ctgctttccg gttcatggaa tgccatcact ctgattgaat caacgttacc
     8881 gttgaaaggt gccgaactgg atcttttaat cgtgatgaag cgcacgacgg cgcgtccgcg
     8941 tccgccaatg ccagcatctg tctgggttca ggtcgatgtt gcagactccc cgcatttaat
     9001 tgaacgcttc acagcacttt tcgacgcgca tcatatgaac attgcggagc tggtgtcgcg
     9061 cacacaacct gctgaaaatg aacgggctgc gcagttgcat attcagataa ccgcccacag
     9121 ccccgcatct gcggacgcag caaatattga gcaagcgttc aaagccctat gtacagaact
     9181 caatgcacaa ggcagtatta acgtcgtcaa ttattcccaa catgatgaac aggatggagt
     9241 taagtaatga atccactgaa agccggtgat atcgcaccga aatttagctt gccggatcaa
     9301 gacggagaac aagttaattt gaccgacttc cagggacagc gtgttctggt ttatttctac
     9361 ccgaaagcca tgacccccgg ctgtaccgta caggcctgcg gcttacgcga taacatggat
     9421 gagttgaaaa aagcgggcgt tgatgtgctg ggtatcagca ccgataaacc cgaaaaactc
     9481 tcccgttttg cggaaaaaga gctgcttaac tttacgctcc tgtctgatga ggaccaccag
     9541 gtgtgcgaac aattcggcgt ctggggtgaa aagtccttca tgggcaaaac ctacgatggt
     9601 attcatcgca tcagcttcct gattgacgct gatggcaaaa tcgaacatgt ctttgacgat
     9661 ttcaaaacca gcaatcacca cgacgttgtg ctgaactggc tgaaagaaca cgcctgatta
     9721 ctttgctcca ttccgtgctg gctgcgcttg cggccagcat acctcacttc tcgtgatcaa
     9781 gatcacattc tcgctttccc ctgcgacacg ggtgtcgaat ccattttttg ctgaacgtta
     9841 atgaccatca tttttgtacc gttcagaatc cagttaatac ataacttatt gaatagattg
     9901 agttaatcag aatggcatcc tttatacaat atgaaatgca atgtttcata tcattttcaa
     9961 ggagccgaca tgaaccgctt tgtggtggcc gaaccactgt ggtgtacagg atgtaatacc
    10021 tgtctcgctg cctgttcgga cgtgcataaa acgcaaggtt tacagcaaca cccgcgcctg
    10081 gccctggcga agacgtcaac aatcactgcc cctgtcgtgt gtcatcactg tgaggaagcc
    10141 ccttgcctgc aggtctgccc ggtcaatgcc atctctcaga gggatgatgc gatccaactc
    10201 aacgaaagcc tctgtattgg ctgcaagctt tgcgccgtgg tctgcccatt tggcgcaatc
    10261 agcgcttcag gaagccgtcc ggtgaatgcc catgcgcaat atgtttttca ggctgaaggc
    10321 tcactcaaag acggcgaaga aaacgtgccg ccccaacatg cgttgctgcg ctgggaaccg
    10381 ggtgtccaga ccgtcgcggt gaaatgcgac ctgtgtgatt tcttgccaga aggtccggcc
    10441 tgcgttcgcg cttgcccgaa tcaggcgtta cggctgatca ccgacgatag cctgcaacgt
    10501 cagatgaaag aaaaacagcg ccttgccgca agctggtttg ccaatggcgg ggagtctccc
    10561 ctttccctca ctcaggagca acactaatgg atgccctgca attattaacc tggtcgctga
    10621 tcctctatct gtttgccagt ctggcttcgc tgtttttact cggtctggac agactggcta
    10681 ttaagctttc cggcatcaca tcgctggtgg gcggcgtgat tggcatcatc agcggaatta
    10741 cgcaattaca tgctggtgta actttagtcg cccattttgc cacccctttt gactttgccg
    10801 atttaaccct gcgaatggat agcctctcgg catttatggt gctagttatc tccttgctgg
    10861 tggtggtttg ttcgctctat tcattgactt atatgcgcga atacgagggc aaaggcgcgg
    10921 cggcgatggg cttctttatg aatcttttca tcgcatcgat ggttgccctg ctggtgatgg
    10981 acaacgcttt ttggttcatc gtgctgtttg aaatgatgtc gctgtcttcc tggtttctgg
    11041 tcattgccag gcaggataaa acgtcgatca acgctggcat gctctacttt tttatcgccc
    11101 acgccggatc ggtgctgatt atgatcgcct tcttgctgat ggggcgcgaa agcggcagcc
    11161 tcgattttgc cagtttcgca cgctttcact ttctccgggg ctggcgtcgg cggtgtttct
    11221 gctggccttt ttcggttttg gcgcgaaagc cgggatgatg ccgttgcaca gctggttgcc
    11281 gcgcgctcac cctgccgcac catcgcacgc ttcggcgttg atgtctggcg taatggtcaa
    11341 aatcggtatt ttcggcatcc tgaaagtagc gatggatctg ctggcgcaaa cgggtttgcc
    11401 tctgtggtgg ggcattctgg tgatggcgat cggcgcaatc tccgcgctcc tgggcgtgct
    11461 atatgcgctg gcggaacagg atatcaaacg gctgctggcc tggagtaccg tcgaaaacgt
    11521 cggcattatt ttgctggcag tcggtgtggc gatggacggt ctgtcactgc acgacccgct
    11581 gctcaccgtg gttggactgc tcggcgcact gtttcatctg ctcaaccatg cgctgttcaa
    11641 agggctgcta tttctcggcg cggacgcgat tatttcgcgt ttgcataccc acgacatgga
    11701 aaaaatgggg gcactggcga aacggatgcc gtggacagcc gcagcatgcc tgattggttg
    11761 cctcgcgata tcagccattc ctccgctgaa tggttttatc agcgaatggt acacctggca
    11821 gtcgctgttc tcactaagtc gtgtggaagc cgtagcgcta caacttgcgg gtcctattgc
    11881 tatggtaatg ctggcagtca ctggtgggct ggcagtaatg tgcttcgtca aaatgtacgg
    11941 tattactttc tgtggtgcgc cgcgcagtac acacgctgaa gaggcacagg aagtgccaaa
    12001 tacgatgatc gtcgccatgc tactgctcgc ggcactctgc gtattaattg cgcttagtgc
    12061 cagttggctg gcaccgaaga taatgcatat tgcccatgcg tttaccaata cccctcccgt
    12121 cactgtcgcc agcggaatag cacttgtacc cggcacgttt catacacggg tcactccttc
    12181 attactgttg ctgttactac tggcgatgcc tttgctgcca ggcctttact ggctgtggtg
    12241 tcgttcgcgc cgcgcagcgt ttcgtcgcac aggagatgcc tgggcatgcg gctacggctg
    12301 ggaaaacgcg atggctccgt caggcaatgg cgtgatgcag ccgctgcgtg tggtcttctc
    12361 tgcgttattt cgtctacgac aacagctcga ccctacgctg aggctaaata aaggtcttgc
    12421 gcacgtcacc gccagggctc agagcacaga acccttctgg gatgagcggg tgatccgccc
    12481 catcgtgagc gccacccaac ggctggtcaa agaaatacag catctgcaaa gcggcgactt
    12541 tcgtctctat tgcctgtatg tggtcgccgc actggttgtg ctgctaatcg ctattgccgt
    12601 ctaaggaaat caccatgaga caaactcttt gcgacggata tctggtcatt tttgcgttag
    12661 cacaggccgt gattctgctg atgctaaccc cactttttac gggtatttcc cggcagatac
    12721 gcgcgcgtat gcactcccgc cgcgggccgg ggatctggca ggattatcgc gatatccaca
    12781 aactgtttaa acgccaggaa gttgcgccga catcttcagg tctgatgttc cgcctgatgc
    12841 cgtgggtatt aatcagcagc atgctggtgc tggcgatggc cttaccacta tttattaccg
    12901 tttccccttt tgcgggcggc ggcgatctga tcacccttat ctatcttctt gccctgtttc
    12961 gttttttctt tgctctttcc gggctggata ccggaagtcc gtttgcggga gtcggtgcca
    13021 gtcgcgagtt gacgctcggc attctggtcg aaccaatgct tattctctca ctgctggtat
    13081 tggcgctgat agcaggttcc acgcatatcg agatgatcag caatacgctg gctatgggct
    13141 ggaactcgcc gctaaccacc gtactggcgt tactggcctg tggttttgcc tgcttcattg
    13201 agatgggaaa aattcccttt gatgttgctg aagcagaaca ggaattacag gaaggcccgc
    13261 tgaccgaata ttccggtgcc gggctggcgc tagcgaaatg ggggctgggg ctgaaacagg
    13321 tcgtgatggc agcactgttt gtggccctgt ttctgccctt tgggcgcgcg caagaacttt
    13381 ctctcgcctg cctgctgact tcacttgtcg ttacgctgct caaggttttg ctgatttttg
    13441 tactggcctc aatcgcagaa aacacgctgg cacgcgggcg ttttttactc attcaccatg
    13501 tgacctggct tggcttcagc cttgctgcgc ttgcatgggt cttttggtta accggtctgt
    13561 aaggagcact gacggaatat ggaaaatctt gctctgacga cgttaatgct gccttttatc
    13621 ggcgcactgg tcgtttcgtt ttcgccacaa cgtcgggccg ccgaatgggg ggttttgttc
    13681 gccgcgctga ccacgctgtg catgttgtcg ctgatctccg cgttttatca ggccgataaa
    13741 gttgccgtca cgttgacgtt ggtcaacgtg ggggatgtgg cattatttgg cctggtcatt
    13801 gatcgcgtga gtacgctgat tctgtttgtg gtggtgtttc tcggtttgct ggtcacgatc
    13861 tactccacgg gttatctgac ggataaaaat cgcgaacacc cgcataacgg cacgaatcgt
    13921 tattacgcat ttttgctggt gtttatcggc gcgatggcgg gactggtact ctcctcgacg
    13981 ctgctcggtc agttgttgtt ttttgaaatt acgggcggct gctcctgggc gttgatcagt
    14041 tattaccaga gcgataaagc gcagcgttca gcactaaaag cgttgcttat cacccatatc
    14101 ggttcgttgg ggttgtatct tgccgccgcc acgctgtttt tgcagaccgg aacgtttgtg
    14161 cttagcgcga tgagcgagtt acatggcgac gcacgttatc tggtttatgg cggcatcctg
    14221 tttgccgcgt gggggaaatc ggcccagcta ccgatgcaag cgtggctacc ggacgcaatg
    14281 gaagcgccaa caccgatcag cgcctatctc cacgccgcat cgatggtgaa agtgggcgtt
    14341 tacatttttg cccgcgccat tatcgacggc ggcaatatcc cgcatgtgat tggcggcgtt
    14401 ggcatggtca tgacactggt caccattctt tatggctttc tgatgtattt gccacagcag
    14461 gatatgaagc ggttgctggc ctggtcgacc atcactcaac ttggctggat gttcttcggc
    14521 ttgtcgctct ccatcttcgg ctcgcggctg gcgctggagg gtagcatcgc ctacatcgtc
    14581 aaccacgcgt tcgctaaaag cctgtttttc cttgtagcag gtgcgctgag ttacagctgc
    14641 ggcacgcgct tgttgccgcg tctgcgtggc gtattgcaca ccctgccgtt gccaggcgtg
    14701 ggtttctgcg tggcagcgct ggcaattacc ggtgtgccgc cgttcaacgg cttcttcagt
    14761 aaattcccgc tgtttgccgc cggttttgcg ttgtcagtgg agtactggat cctgctgccc
    14821 gccatgattc tgctgatgat tgaatcggtc gccagtttcg cctggtttat tcgctggttt
    14881 ggtcgcgtcg tgccgggcaa accgagcgag gccgtcgccg atgccgcacc gctgccaggg
    14941 tcaatgcgcc tggtgttgat tgtactgatt gtgatgtcgc tgatttccag cgtaatcgcc
    15001 gcgacctggt tgcagtaagg agatgatgaa tgaccggttc tatgatcgta aataatctgg
    15061 cgggactgat gatgctgaca tcgctgtttg tgattagcgt caaaagctat cgcctgtcat
    15121 gcggatttta cgcctgccag tcgctggtgc tggtgtctat tttcgccact ctctcgtgcc
    15181 tgttcgccac agagcaactg ctgatctggt ccgccagcgc ctttatcacc aaagtgctgc
    15241 tggtaccgtt aatcatgact tacgctgcac gaaatattcc ccagaacatc ccggaaaaag
    15301 cgttattcgg tccggcaatg atggcactgc tcgcggcgtt aattgtcctg ctttgcgcat
    15361 ttgtcgttca gcccgtgaag ctaccgatgg ctaccgggct gaaaccggcg ctggcggtag
    15421 cgttaggtca ttttctgctt ggcctgctgt gcattgtcag ccaacgcaat atcctgcggc
    15481 aaatttttgg ttactgcctg atggaaaacg gctcccatct ggtgctggcg cttcttgcct
    15541 ggcgagcacc ggaactggtg gaaataggta tcgctaccga cgccatcttc gccgtcattg
    15601 tgatggtgtt actggcaaga aaaatatggc gtacccacgg cacgctggac gtgaacaact
    15661 tgaccgcgct gaagggataa tgagatgagt tattctgtga tgttcgcttt actcctgctc
    15721 acgccgctgc ttttttcgct gctctgtttt gcctgccgga aacgggggct ctctgcgact
    15781 tgtacggtga ctgtattaca tagcttaggg atcacactgc tgctgattct agcactctgg
    15841 gtggtccaaa ctgccgctga tgcaggagaa atattcgctg cgggactgtg gcttcatatt
    15901 gatggtctgg gcggtttgtt cctcgccatt cttggtgtga ttggctttct caccggtgtt
    15961 tactcgattg gctacatgcg tcatgaagtg gcacacggcg agctttcacc cgttacactg
    16021 tgcgattact acggtttctt ccatctgttt ttgttcacca tgctgctggt tgttaccagc
    16081 aataacctga ttgtgatgtg ggcggcgatc gaagccacca ccttaagctc ggcgtttctg
    16141 gtaggcattt acggtcagcg ttcatcgctg gaatcagcat ggaagtacat cattatttgt
    16201 actgttggtg tcgcttttgg tctgttcggt accgtgctgg tatacgccaa cgccgccagc
    16261 gttatgccgc aggcagaaat ggcgatattc tggagcgagg ttcttaagca atcgtccttg
    16321 cttgacccaa cattaatgct gttggccttt gtgtttgtgc taattggctt tggtaccaaa
    16381 accgggctat ttcccatgca cgcctggctg ccggatgctc acagtgaagc gccgagtccg
    16441 gtcagcgccc tgctctccgc cgtattgctg aactgcgcgc tgttggtgct gattcgctat
    16501 tacatcatta tttgccaggc catcggcagc gatttcccca accggttgtt gctcatcttc
    16561 ggcatgttgt cggttgccgt ggcggcattt ttcattctgg tacagcggga cattaagcgt
    16621 ctgctggcgt actccagcgt ggagaacatg gggctggtcg cggtggcgct aggcattggc
    16681 gggccgctgg gaatttttgc cgcgctgctg cacaccttaa accacagtct ggcaaaaacg
    16741 ctgctgttct gcggttccgg caatgtactg ctcaagtacg gcacgcgcga tctcaacgtc
    16801 gtctgtggga tgctcaaaat catgccattt accgccgtgc tgtttggcgg cggtgcgctg
    16861 gcgctggcgc tggcgctggc agggatgccg cccttcaaca tttttcttag cgaatttatg
    16921 accgttaccg caggactggc acgtaatcac ctgctgatta tcgtcctgct gttattgctg
    16981 ttaacgctgg tgctggcggg cctggtacgg atggctgcgc gggtgttaat ggcgaaaccg
    17041 ccgcaggccg ttaaccgggg tgatctcggc tggttgacca cctcgccaat ggtgattctg
    17101 ctggtcatga tgctggcgat gggaacgcat attccacaac ctgtcatcag gatcctggcg
    17161 ggcgcttcca ctatagtcct ctcagggacg cacgacctgc ctgcacaacg tagcacctgg
    17221 catgattttt tgccttcagg caccgcatct gtttcggaga aacacagtga acgttaattc
    17281 atcgtcaaat cgtggcgaag cgattctcgc cgccctgaaa acgcagttcc ccggcgcggt
    17341 gctggatgaa gagcgacaaa cgcctgaaca ggtcaccatt acggtgaaaa tcaatctgct
    17401 gcctgacgtt gtacattatc tttattatca acatgatggc tggcttccag tcctgtttgg
    17461 caacgacgag cggacactta acggtcatta cgcggtttat tatgcccttt caatggaagg
    17521 ggccgaaaaa tgctggatcg tggtgaaggc actggtcgat gccgacagtc gggagtttcc
    17581 gtcagtcaca ccgcgcgtcc ctgccgcggt ctggggcgag cgagaaattc gcgatatgta
    17641 cgggctgatt ccggttggcc tgccggatca gcgtcgcctg gtgttgcccg atgactggcc
    17701 ggaagatatg catccgctgc gcaaagatgc gatggattat cgactgcgcc ctgaaccgac
    17761 gacagattcc gaaacgtatg cgtttatcaa tgagggcaac agcgatgcgc gggtgatccc
    17821 tgtcggcccg ctgcatatca cctcctatga accgggtcac ttccgcttgt ttgtggatgg
    17881 cgagcaaatt gtcgatgctg attaccgcct gttttatgtc catcgcggca tggagaaact
    17941 ggcagaaacg cggatgggct acaacgaagt gaccttctta tcggaccgcg tgtgtgggat
    18001 ttgcggtttt gcccacagtg tggcctatac caactcggtt gaaaatgcac tggggattga
    18061 ggtgccgcaa cgagcacata ctattcgctc gattctgctg gaagtcgagc ggctacacag
    18121 tcatttgctc aaccttggcc tctcctgcca ttttgttggt tttgataccg gctttatgca
    18181 atttttccgc gtgcgggaaa agtcgatgac gatggcggaa ttgctgaccg ggtcgcgtaa
    18241 aacctacggt ctgaatctga ttggtggtgt tcgccgcgat attctcaaag agcaacgtct
    18301 gcaaacgctg aaactggtgc gcgagatgcg cgccgacgtg tcggagctgg tagagatgct
    18361 gcttgctacg ccgaatatgg aacaacgcac tcagggcatt ggcattctcg accgacaaat
    18421 cgcccgtgat tatagccctg tagggccgct gatccgcgca gtggttttgc ccgtgatttg
    18481 cgctttgatc acccctacgc cgactacggc aatattccaa aaacactgtt tacctttacc
    18541 ggcggcgatg ttttctcccg cgtgatggtc cgtgtcaaag agacgtttga ttcgctggca
    18601 atgctggaat ttgccctcga caacatgccg gataccccac tgctgaccga aggctttagc
    18661 tataaacctc acgcattcgc gctgggcttt gttgaagcgc cacgcggtga agacgtgcac
    18721 tggagcatgc tcggtgataa ccaaaaattg ttccgctggc gctgtcgtgc cgccacctac
    18781 gccaactggc cggtgttgcg ttacatgctg cgcggcaata ccgtttctga cgcaccgctg
    18841 attatcggta gccttgatcc ctgctactcc tgtaccgacc gtgtgacgct ggtagatgtg
    18901 cgcaagcgcc agtcaaaaac cgtgctgtat aaagagatcg aacgctacgg cattgatcgt
    18961 aaccgttcgc cgctgaagta aggacagaag atgctgaagt tactgaaaac tattatgcgc
    19021 gccggaaccg cgacggtgaa atatcccttc gcgccactgg aggtcagccc aggctttcgc
    19081 gggaaaccgg acctgatgcc cagccaatgt attgcctgcg gtgcctgcgc ctgtgcttgt
    19141 ccggcaaatg cgctgactat ccagaccgac gaccagcaaa attcgcgcac ctggcagctc
    19201 tatctggggc gttgtattta ctgcggacgt tgtgaagaag tgtgcccgac cagagccatc
    19261 cagcttacca ataactttga actgaccgtc acccataaag ccgatctcta tacccacgcg
    19321 acgttccatc tacaacgttg cagccgttgc gaacgcccgt ttgccccgca aaaaaccgtc
    19381 gcactggctg ctgaattgtt ggcacagcaa caaaatgctc cacaaaaccg cgaaatgtta
    19441 tgggcgcaag cgagcgtctg cccggagtgc aaacaacgcg caacgctgat caacgacgat
    19501 acagatgtac cgctggtggc taaggagcag ctatgagtcc agtgcttacg caatatgtca
    19561 gccagcccat cacgctggac gagcaaacgc aaaagatgaa gcggcatttg ctacaggata
    19621 tccgtcgctc ggcttacgtt tatcgcatcg attgcggcgg ctgcaacgcc tgtgaaatcg
    19681 aaatttttgc tgccatcacc cccgtattcg acgcagaacg ttttggcatt aaggttgttt
    19741 catcaccgcg tcacgccgat attttgttat ttactggcgc agtcacccgg gcgatgcgta
    19801 tgcctgcact tcgggcgtat gagtctgccc ccgatcataa aatctgtgtt tcctacggcg
    19861 cgtgcggtgt cggcggcggt attttccacg atctctacag cgtctggggc ggcagcgaca
    19921 ccattgtccc cattgatgtt tggatccccg gctgcccgcc aacaccggcc gccaccattc
    19981 acggtttcgc cgtggcgctc ggtttgctgc aacagaagat tcacgcggtg gattatcgcg
    20041 atcccaccgg ggtgactatg caaccgttgt ggccgcagat cccgccatca cagcgtatcg
    20101 ccattgagcg agaagcgcgg cggctggcgg gctatcgtca ggggcgagaa atttgcgatc
    20161 ggctcctgcg ccatttaagc gacgatccta caggaaatcg ggttaacacc tggttgcgcg
    20221 atgccgacga tccacgtctc aatagtatcg ttcagcaact ctttcgcgta ctccgggggt
    20281 tacatgactg aagagtgcgg ggaaattgtt ttctggacgc tgcgaaaaaa gtttgtcgcc
    20341 agtagcgacg agatgccgga acacagctct caggtaatgt attactcgct ggctatcggt
    20401 catcacgttg gcgtgattga ttgtctgaat gtcgccttcc gctgcccact gacggaatac
    20461 gaagattggc ttgcactggt cgaagaggag caagcccgac gtaagatgct gggggtgatg
    20521 acttttggtg agattgttat tgacgccagc cacaccgccc tgttgacccg ggcattcgcg
    20581 ccactggcag atgacgcgac gtctgtgtgg caagcgcgta gcattcaatt cattcatctg
    20641 ttggatgaaa ttgtgctgga accggctatc tatctgatgg ccagaaaaat tgcgtgagaa
    20701 ggatttctca ttaataagga ctgttgatgg ctatgtcaga cgaagcgatg tttgccccgc
    20761 cgcaaggaat aacaattgaa gcggtaaacg gaatgctcgc ggagcggtta gcgcagaaac
    20821 acggcaaggc gtctttatta cgcgccttca tcccgctgcc gccgccgttc agcccggtac
    20881 aacttattga actgcatgtt ctcaaaagca acttctatta ccgctaccat gatgatggca
    20941 gcgatgtgac ggcaacaaca gagtatcagg gcgagatggt cgattattcg cgttacgccg
    21001 tccttctcgg cagtagtgga atggcggagc tacgctttat tcgtacccac ggcagccgtt
    21061 ttactcccca ggattgcaca ctgtttaact ggctggcacg cattatcacc ccggttctgc
    21121 aatcatggct caatgatgaa gcacagcagg tggcgctgcg tttgctggag aaagatcgcg
    21181 atcatcatcg ggtactggtg gatatcacta atgcagtgct gtcacatctt gatctcgacg
    21241 atctgatcgc tgacgtcgct cgtgagatcc atcatttttt cggtctggct tcagtcagta
    21301 tggtactggg cgatcatcga aagaacgaga agttcagcct gtggtgcagc gatctttctg
    21361 cctcacattg tgcgtgtctg ccacacaata tgcctggcga cagtgtattg ctgacacaaa
    21421 cgctacaaac ccgacaaccg accttgacgc accgtgcaga tgatctgttt ctctggcaac
    21481 gcgacccgtt attactctta cttgcatcta acggctgcga atctgcgctc cttataccgc
    21541 ttacctttgg caaccataca ccgggtgcat tgttgctggc gcatacctct tccactctct
    21601 ttagtgagga aaactgccag ctactacaac acatagccga tcgcatcgct attgccgttg
    21661 gcaatgccga tgcctgccgt agaatgaccg atttgcagga aagtttgcag caagaaaacc
    21721 accagcttag cgagcagctc ctttcgaatc tgggcgtcgg tgacattatc tatcaaagcc
    21781 aggcaatgga agacctgctc cagcaggtag atattgtggc gaagagcgac agtacggtgt
    21841 tgatttgcgg tgaaaccgga accggcaaag aggtgatcgc cagagcgatc catcaactta
    21901 gcccgcgaca cgacaagccg ctggtcaaaa tcaactgcgc tgccatcccc gccagtcttc
    21961 tggaaagtga gttattcggt catgacaaag gggcgtttac tggtgcgatt aatacccatc
    22021 gtggtcgttt tgaaattgcc gatggcggca cgttgtttct cgatgaaatt ggcgatctgc
    22081 cgttagaact tcagcctaaa ctgctgcgcg tattgcagga acgggagatt gagcgtctcg
    22141 gcgggagtag aacgatcccg gtaaatgtca gagtcattgc cgccaccaac cgtgatttgt
    22201 ggcaaatggt tgaagatcgc cagtttcgca gcgatctctt ttatcgcctg aatgtcttcc
    22261 cactggaatt gcctccgctg cgcgaccgtc cggaagatat ccctctttta gcaaagcatt
    22321 tcacgcaaaa aatggcgcgc catatgaatc gttcaattga cgccatcccg accgaggcgc
    22381 tacgccagtt gatgtcgtgg gactggccgg gcaacgtgcg cgagctggaa aacgtgattg
    22441 agcgagcggt actgctgact cgcggtaaca gtctgaattt acatcttaat gtccgacaaa
    22501 gccgtttact gccgacgcta aatgaagatt cagcgcttcg cagttcaatg gcgtagttgc
    22561 tgcacccgac gacgccagag aatgacgaag aagaacgtca gcgcattgtt caggtattgc
    22621 gagaaaccaa tggcattgtt gccgggcccc gtggcgcggc gacacgatta gggatgaagc
    22681 gcaccacgct gctgtcacga atgcagcgtc tgggaatctc ggttcgcgag gtgttgtaat
    22741 ctgcttttgc aggagtatgc atgagaaaca aactctcttt cgacttgcag ttgagcgcca
    22801 gaaaagcggc aatcgctgaa cggattgccg cccataaaat tgcccgcagt aaagtgtcgg
    22861 tctttttaat ggcgatgtcc gctggcgtgt ttatggcgat gtccgctggc gtgtttatgg
    22921 cgatcggatt tactttttac ctttccgtta tcgccgatgc cccgtcttca taggcattaa
    22981 cccatctggt gggcggcctt tgctttacac ttggctttat tttgctggcg gtttgcggca
    23041 ccagcctgtt cacctcgtcg gtaatgacgg taatggcaaa aagtcggggc gttattagtt
    23101 ggcgaacttg gctgattaac gcacttctgg tggcctgcgg taatctggca ggtattgcct
    23161 gtttcagttt gttaatctgg ttttccgggc tggtgatgag tgaaaacgcg atgtggggag
    23221 tcgcgatttt acactgcgcc gagggcaaaa tgcatcatac atttactgaa tctgtcagcc
    23281 tcggcattat gtgcaatctg atggtttgcc tggcgctgtg gatgagttat tgcggacgtt
    23341 cgttatgcga caaaatcgtc gccatgattt tgtccatcac cctgtttgtc gccagtggct
    23401 ttgagcactg tatcgccaat ttgtttgtga ttccgttcgc cattgccatt cgccatttcg
    23461 cccctacctc cttctggcaa ctggcgcaca gtagcgcaga caattttccg gcactgacgg
    23521 tcagccattt tattaccgcc aatctgctcc cggtgatgct gggtaatatt atcggcggtg
    23581 cagtgctggt gagtatttgt tatcgggcta tttatttacg tcaggaatcc tgaaaaatca
    23641 gcccggcgaa acagttcgtc gggctattta tttacgtcag gaatcctgaa aaatcagccc
    23701 ggcgaaacag ttcgtcgggc tgaagattat tcttgcgcga tttgcccatc gggccaggcg
    23761 tggatcacgg ctttgatcag cgtcgccaat ggaatggcga agaatacgcc ccagaatccc
    23821 cacaaaccac cgaagatcac caccgataaa ataatcacca gcggatgcag gttaaccgct
    23881 tcggagaaca acaccggtac tagcaggttg ccgtccagcg cctgaataat cagatacacc
    23941 gcgaagcagc tccagaattc agtacctgcg ccaaactgga atagcgccac gccaaccacc
    24001 ggaatggtca ccacaaatgc gccaatgtac ggaataagaa ccgagaagcc gaccagcact
    24061 gccagcagca gcgaatagtt cagcccgaag agcaagaacc ccagccaggt ggcgatcccc
    24121 accacgatca tctccagcac tttgccgcgg atatagttgg tgatttgttg attcatctcc
    24181 ttccacacct gtcctgccag cccacggtta cgcggcagca cccggcgaac ggcgttcagc
    24241 atctgctctt tgtctttcag caggaagaag accatcaatg gcaccagcac cagatagacg
    24301 gctatggtca gcaaaccgac cagcgaggcg agggaaattt tcaccaccga atcgcccatg
    24361 gtcagcatcc gactgcgcat attttcggcc attgcatcaa taatgcccgc gtccattaac
    24421 accggatagc ggcgcggcaa cgtggcggca aagtcagaaa gcttattgag catccccggc
    24481 atatcgcgga ttaagtagat gccctgttgc caggcgatag gcagtaccac gaacgccatc
    24541 agtagcaata taccgacaaa aaccaccaat acaatcgacg tcgcccagcg gcgggagcag
    24601 ccaatagatt gcaggcgcac ggttggccat tccagcaaat aagccagcac aatagccacc
    24661 agcaacggag caagcaggcc actaaagaaa aagataatgc caaatccggc aactaaaata
    24721 accagcaagg caatcgcttc cgggtcgcta aaacggcggc gataccattg catcaacatt
    24781 tcgagcataa agaccttccc tgaacctcaa gagcgggatt gcgatccgca attgtatcga
    24841 aatgtcacaa aaaagacttc gctttttatg acggattcag gaaactgaaa agtcatttga
    24901 gtgggctaat cttcgccgtt acactcaaag gcggcgaggt gggaacgata tttcacagta
    24961 tcggtcaaat gactatcttc agaaatacag gatagaggtt atgttcaggc agttgaaaaa
    25021 aaacctggtt gcaaccctca ttgctgctat gaccattggt caggtagccc cggcgtttgc
    25081 cgacagcgca gacaccttgc cggatatggg aacctccgca ggaagcacgc tttccattgg
    25141 tcaggaaatg cagatgggcg actattatgt ccgccagcta cgcggcagcg cgccgttaat
    25201 taatgacccg ctgttaacgc aatatattaa ttcgctgggg atgcgtctgg tttcgcatgc
    25261 caattcggtt aagacaccgt ttcatttttt tctgatcaac aacgacgaaa ttaacgcctt
    25321 tgctttcttt ggcggcaacg tggtgctgca ctctgccctg ttccgttatt ccgataacga
    25381 aagtcaactg gcttcagtta tggcgcacga aatctcccac gtcacccaac gtcacctggc
    25441 gcgagcgatg gaagatcaac aacgcagcgc gccgctgacc tgggtcggcg cgttaggttc
    25501 tattttactg gcgatggcca gtccgcaggc ggggatggcg gcgctgaccg gtacactggc
    25561 gggaacgcgc caggggatga tcagtttcac ccagcaaaat gaacaggaag cggaccgcat
    25621 tggtattcag gtgctgcaac gctcgggatt cgatccacag gcgatgccga ccttcctgga
    25681 aaaattgctc gatcaggcgc gttactcctc gcgcccgccg gaaattttat tgactcaccc
    25741 gttgccggaa agtcgtctgg cagatgcccg caaccgtgct aatcagatgc gcccgatggt
    25801 ggtgcagtcg tcggaagatt tctatctggc gaaagcgcgc acactgggga tgtataattc
    25861 cggacgtaac cagctcacca gtgatttgct ggatgaatgg gcgaaaggaa acgttcgtca
    25921 gcaacgagcg gcgcaatatg gtcgtgcttt acaggcgatg gaagccaata aatacgacga
    25981 ggcgcgtaaa acgctgcaac cgttactggc ggcagaacct ggcaacgcat ggtatctcga
    26041 tctggctacc gatatcgatc ttgggcaaaa caaagccaat gaggcaatca atcgcctgaa
    26101 aaatgcccgc gatttacgca ccaatccggt gttgcagctc aacctggcga acgcttatct
    26161 gcaaggcggt caaccacaag aagcggccaa tattctgaat cgctacacct ttaataataa
    26221 agatgacagc aacggctggg atttgctggc acaggcggaa gccgcgctaa ataaccgcga
    26281 tcaggagctg gctgcgcgag cagaaggtta tgcgctcgcc ggacgactcg atcaggccat
    26341 ttcgctgttg agtagcgcca gttcgcaggt gaaattaggc agcctgcaac aagcgcgtta
    26401 cgatgcgcgc atcgaccagt tgcgccagct gcagaaacgc tttaagcctt ataccaagat
    26461 gtaattcgca ttaaaggaag aacaatgacc aaacaggtaa aaatctacca taacccacgc
    26521 tgttcaaaga gccgggaaac gctgaacctg ctgaaagaaa acggcgtgga gccagaagtg
    26581 gtgctctacc ttgagacacc cgccgatgcg gcaacgctgc gcgatttgct gaaaatgctg
    26641 gggatgaata gcgcccgtga attgatgcgc cagaaagagg atctttataa agaactaaac
    26701 ctggctgaca gttcgcttag cgaagaggcg ctgattcagg caatggtgga taatccgaag
    26761 ctgatggagc gcccgattat ggtcgcgaat ggcaaagcgc ggattggtcg tccaccggag
    26821 caggtactgg aaatcgtggg ttaagatgtg ctccatcgct agttgaagca cattgccgga
    26881 tgcgacgctt gccacgtctt atccggccta cgaatactat ggattaactt cgcgtaggtc
    26941 aggcgttcgc gccgcatccg acaataaaca ccttatctac aacttcagaa tttctttcac
    27001 aaacggaatg gtcagcttac gttgcgcggt aatcgacgca cgatccaact gatccaacgt
    27061 cataaatagc gtgcgcattt ctctgtcgag ccgcttcagc aagaaacgcc ccacatcttc
    27121 cggcagttca aaaccacgca aacgcgcgcg taactgtagc gcctgcaact tatcttcatc
    27181 agaaagtggc tgcaatttgt agatctgccc ccagtcgaga cgcgacgcga gatccggtaa
    27241 tcccagattc aactgccgcg gtggacgatc gccggtgatc aacaaccgtg ttttgcccga
    27301 ttccagaatt cgattgtaga gatcgaaaat cgccatctcc cacaactcat cgcctgcaat
    27361 acactcaatg ttgtcgatac agaccagcga caaatgctcc ataccgtcga gcacttccgg
    27421 aacaaaccag gtgcgtttat ccagcgggac atagcccacc gcatcgccac gctgcgacaa
    27481 ttccgcgcaa gccgcgtgca gcagatggct gcgccccgcg ccttcgcgtg cccagagata
    27541 gatgtaaccg ctatgttcct gacgcagcac gttttgcagc gcggccagta aagaggagtt
    27601 atcccccggc cagaaacttg caaaggtttc gtcgtcagga agataaagtg gcaaagagag
    27661 ctgtgccggt gtgttcagag atacctcaac caggatttca caaaatcgcg agaagtttac
    27721 cacagaatcc catgatgttt gaaccgggca gcaacactgc ccggtcggta cattatttgt
    27781 ctgttatatc cgcgtcttct gcgtccagca ccacttcttc agggcgcagc acactgatca
    27841 gtttgaaaat caaacttaag ccgataccga cgatggtcgc cagcgccata ccttttaact
    27901 ccgccgcgcc gatgtttacc ttcgcgccac tgacgccgat gatcaaaatc acggaagtca
    27961 ggatcaggtt ctgtgctttg ttgtaatcca ctttcgattc gatcaaaaca cgaataccgg
    28021 aagcaccaat gacaccataa agcagcagcg aaacgccgcc catcaccggc aatgggatca
    28081 tctggatagc ggcagccagt ttaccgacgc aggaaagcag gatagcgaaa atcgccgccc
    28141 cgccgataac ccaggtactg taaacacggg tgatcgccat cacgccaatg ttttctccgt
    28201 aagtggtatt tggcgtagag ccaaagaagc cggagatcac ggtcgacagg ccgttagcaa
    28261 acatcgaacg gtgcagacct ggatcgcgca gcagatcttt tttgacgata ttagccgtta
    28321 ctaccaggtg ccctacgtgt tcggcaataa ccactaacgc cgctggcaga atagtcagaa
    28381 tggcaaacca ctcgaagcgc ggcgtataga gggttggcag tgcaaaccag tgagcattaa
    28441 taatcggcgt ggtatcgaca attcccattg cgaaagagag cgcgtacccc accagcacgc
    28501 caattaaaat cgggataatt gccaggaaac cacgaaacag cacggaacct aaaaccgtga
    28561 ccgccagggt ggtaatagag atgatgatgg ttttggagtt tggcgtttgc ccttcagccg
    28621 ggagtaaacc cgccataccg gcagctacgc ccgccagctc cagaccgatg acggcaacga
    28681 ttgcgcccat tgccgcaggt ggaaacagca cgtccaacca gccggtcccc gctttcttca
    28741 cgataaaaga aaccaggcag aacagcacgc cgcacataat aaagccgccc agcgcgactt
    28801 cataccctaa cggcaacagt aacaataccg gtgaaataaa ggcaaagctg gaaccaagat
    28861 aagccggaat tttcccttta cagatgaaga gatacagcag cgttccaata ccgttaaata
    28921 acagtacagt cgccgggtta atatgaaata agacgggcac caggacggtt gcaccaaaca
    28981 tggcgaacaa atgttgcaaa ctaagcggga ttgtctgtaa aagtggcggt ctttcactca
    29041 ccccgatagc acggcgcgtc atagtattat cctctgtatt atgtgttata ggcgctttac
    29101 tcaaaaaaaa gccgactctt aaagtcggct ttaattattt ttattcttta tttcgtacca
    29161 aagattttgt caccggcatc gccgaggccc ggaataatgt atccgtgctc gttcagtccc
    29221 tggtcaatcg atgcagtata cagttcgacg tccgggtgcg ctttttccag cgcagcgata
    29281 ccttctggcg cagcgaccag caccagaact ttgatgctgc tgcagcccgc ttttttcagc
    29341 aggtcgatgg tcgcgataac ggaaccaccg gttgccagca ttgggtcaac gatcagcgcc
    29401 atacgctcat cgatgttaga aaccagtttc tggaagtacg gtaccggctc cagcgtttct
    29461 tcattacggt acataccgac aacgctgatg cgcgcgctcg gaacgttttc cagcacaccg
    29521 tccatcatac caagacccgc acgcagaatt ggcacaacgg taattttctt acctttgatc
    29581 tggtcgattt ctaccgggcc gttccagcct tcgatagtta ctttttccgt ttcgaggtcg
    29641 gcggtcgctt cgtaagtcag caggctaccc acttcggaag cgagttcgcg aaagcgcttg
    29701 gtgctgatat cttgctcacg catcagtccc agcttgtgtt tgacgagtgg gtgtttgact
    29761 tccacgatct tcatactctt tctcctttga ggggcagcca caaaaaaatc gacggattat
    29821 acctcctttc ttcagggcgg caatattatt ttcgttgact ttagtcaaaa tgataacagt
    29881 ttgagataaa gttattttat attcaaatgg ttatgaaaga agattattcc atccgaaaac
    29941 taacctttgc cctggcacaa gtcttctttc gccgcgcgcc cggggaaaag acgtgcaaaa
    30001 aggttgtgta aaacagtctc gcaaacgttt gctttccctg ttagaattgc gccgaatttt
    30061 atttttctac cgcaagtaac gcgtggggac ccaagcagtg accgataaaa cctctcttag
    30121 ctacaaagat gccggtgttg atattgacgc gggtaatgct ctggttggaa gaatcaaagg
    30181 cgtagtgaag aaaacgcgtc gtccggaagt gatgggcggt ctgggcggct tcggtgcgct
    30241 gtgtgcattg ccgcaaaaat atcgtgaacc cgtgctggtt tccggcactg acggcgtagg
    30301 taccaagctg cgtctggcaa tggacttaaa acgtcacgac accattggta ttgatctggt
    30361 cgccatgtgc gttaatgacc tggtggtgca aggtgcggaa ccgctgtttt tcctcgacta
    30421 ttacgcaacc ggaaaactgg atgttgatac cgcttcagcg gtgatcagcg gcatcgcgga
    30481 aggttgtctg caatcgggct gttctctggt gggtggcgaa acggcagaaa tgccggggat
    30541 gtatcacggt gaagattacg atgtcgcggg tttctgcgtg ggcgtggtag aaaaatcaga
    30601 aatcatcgac ggctctaaag tcagcgacgg cgatgtgctg attgcactcg gttccagcgg
    30661 tccgcactcg aacggctatt cgctggtgcg caaaattctt gaagtcagcg gttgtgatcc
    30721 gcaaaccacc gaacttgatg gtaagccatt agccgatcat ctgctggcac cgacccgcat
    30781 ttacgtgaag tcagtgctgg agttgattga aaaggtcgat gtgcatgcca ttgcgcacct
    30841 gaccggcggc ggcttctggg aaaacattcc gcgcgtattg ccagataata cccaggcagt
    30901 gattgatgaa tcttcctggc agtggccgga agtgttcaac tggctgcaaa cggcaggtaa
    30961 cgttgagcgc catgaaatgt atcgcacctt caactgcggc gtcgggatga ttatcgccct
    31021 gcctgctccg gaagtggaca aagccctcgc cctgctaaat gccaacggtg aaaacgcgtg
    31081 gaaaatcggt atcatcaaag cctctgattc cgaacaacgc gtggttatcg aataatgaat
    31141 attgtggtgc ttatttccgg caacggaagt aatttacagg caattattga cgcctgtaaa
    31201 accaacaaaa ttaaaggcac cgtacgggca gttttcagca ataaggccga cgcgttcggc
    31261 cttgaacgcg cccgccaggc gggtattgca acgcatacgc tcatcgccag cgcgtttgac
    31321 agtcgtgaag cctatgaccg ggagttgatt catgaaatcg acatgtacgc acccgatgtg
    31381 gtcgtgctgg ctggttttat gcgcattctc agcccggcgt ttgtctccca ctatgccggg
    31441 cgtttgctga acattcaccc ttctctgctg ccgaaatatc ccggattaca cacccatcgt
    31501 caggcgctgg aaaatggcga tgaagagcac ggtacatcgg tgcatttcgt caccgatgaa
    31561 ctggacggtg gcccggttat tttacaggcg aaagtcccgg tatttgctgg tgatacggaa
    31621 gatgacatca ccgcccgcgt gcaaacccag gaacacgcta tttatccact ggtgattagc
    31681 tggtttgccg atggtcgtct gaaaatgcac gaaaacgccg cgtggctgga tggtcaacgt
    31741 ctgccgccgc agggctacgc tgccgacgag taatgccccc gtagttaaag cgccagctct
    31801 gccgctggcg tttttcaatt cacctgtaaa tcgcaagctc cagcagtttt tttccccctt
    31861 ttctggcata gttggacatc tgccaatatt gctcgccata atatccaggc agtgtcccgt
    31921 gaataaaacg gagtaaaagt ggtaatgggt caggaaaagc tatacatcga aaaagagctc
    31981 agttggttat cgttcaatga acgcgtgctt caggaagcgg cggacaaatc taacccgctg
    32041 attgaaagga tgcgtttcct ggggatctat tccaataacc ttgatgagtt ctataaagtc
    32101 cgcttcgctg aactgaagcg acgcatcatt attagcgaag aacaaggctc caacgctcat
    32161 tcccgccatt tactgggcaa aattcagtcc cgggtgctga aagccgatca ggaattcgac
    32221 ggcctctaca acgagctatt gctggagatg gcgcgcaacc agatcttcct gattaatgaa
    32281 cgccagctct ccgtcaatca acaaaactgg ctgcgtcatt attttaagca gtatctgcgt
    32341 cagcacatta cgccgatttt aatcaatcct gacactgact tagtgcagtt cctgaaagat
    32401 gattacacct atctggcggt ggaaattatc cgtagcgata gcatccgtta cgcgctgctg
    32461 gagatcccat cagataaagt gccgcgcttt gtgaatttac cgccagaagc gccgcgtcga
    32521 cgcaagccga tgattcttct ggataacatt ctgcgttact gccttgatga tattttcaaa
    32581 ggcttctttg attatgacgc gctgaatgcc tattcaatga agatgacccg cgatgccgaa
    32641 tacgatttag tgcatgagat ggaagccagc ctgatggagt tgatgtcttc cagtctcaag
    32701 cagcgtttaa ctgctgagcc ggtgcgtttt gtttatcagc gcgatatgcc caatgcgctg
    32761 gttgaagtgt tacgcgaaaa actgactatt tcccgctacg actccatcgt ccccggcggt
    32821 cgttatcata attttaaaga ctttattaat ttccccaatg tcggcaaagc caatctggtg
    32881 aacaaaccac tgccgcgttt acgccatatt tggtttgata aagcccagtt ccgcaatggt
    32941 tttgatgcca ttcgcgaacg cgatgtgttg ctctattatc cttatcacac ctttgagcat
    33001 gtgctggaac tgctgcgtca ggcttcgttc gacccgagcg tactggcgat taaaattaac
    33061 atttaccgcg tggcgaaaga ttcacgcatc atcgactcaa tgatccacgc cgcacataac
    33121 ggtaagaaag tcactgtggt ggttgagtta caggcgcgtt tcgacgaaga agccaacatt
    33181 cactgggcga agcgcctgac cgaagcaggc gtgcacgtta tcttctctgc gccggggctg
    33241 aaaattcacg ccaaactgtt cctgatttca cgtaaagaaa acggtgaagt ggtccgttac
    33301 gcacacatcg ggaccgggaa ctttaacgaa aaaaccgcgc gtctttatac tgactattcg
    33361 ttgctgaccg cagatgcgcg tatcaccaac gaagtacggc gggtatttaa ctttattgaa
    33421 aacccatacc gcccggtgac atttgattat ttaatggtgt caccgcaaaa ctctcgccgt
    33481 ctgttatatg aaatggtaga ccgcgaaatc gccaacgcgc agcaagggct gcccagtggt
    33541 atcaccctga agctaaataa ccttgtcgat aaaggcctgg ttgatcgtct gtatgcggcc
    33601 tccagctccg gcgtaccggt taatctgctg gttcgcggaa tgtgttcgct gatccccaat
    33661 ctggaaggca ttagcgacaa cattcgtgcc atcagtattg ttgaccgtta ccttgaacat
    33721 gaccgggttt atatttttga aaatggcggc gataaaaagg tctacctttc ttccgccgac
    33781 tggatgacgc gcaatattga ttatcgtatt gaagtggcga caccgctgct cgatccgcgc
    33841 ctgaagcagc gggtgctgga catcatcgac atattgttca gcgatacggt caaagcacgt
    33901 tatatcgata aagaactcag taatcgctac gttccccgcg gcaatcgccg caaagtacgg
    33961 gcgcagttgg cgatttacga ctacatcaaa tcactcgaac aacctgaata accctatgcc
    34021 aatacacgat aaatcccctc gtccgcagga gtttgctgcg gtcgatcttg gttcaaacag
    34081 ttttcacatg gtcatagccc gtgtggtaga tggtgccatg cagattattg gccgcctgaa
    34141 acagcgggtg catctggcgg acggcctggg gccagataat atgttgagtg aagaggcaat
    34201 gacgcgcggt ttgaactgtc tgtcgctgtt tgccgaacgg ctacaagggt tttctcctgc
    34261 cagcgtctgt atagtcggca cccatacgct gcgtcaggcg ctgaacgcca ctgactttct
    34321 gaaacgcgcg gaaaaggtca ttccctaccc gattgaaatt atttccggta atgaagaagc
    34381 ccgtctgatt tttatgggcg tggaacatac ccaaccggaa aaaggtcgca aactggttat
    34441 tgatattggc ggcggatcta cggaactggt gattggtgaa aatttcgaac ctattctcgt
    34501 tgaaagccgc cggatgggtt gtgtcagctt tgcccagctt tattttcctg gcggggtcat
    34561 caataaagag aattttcagc gcgctcgcat ggcggcagca caaaaactgg aaactttaac
    34621 ctggcaattc cgtattcagg gctggaacgt tgcaatgggc gcttccggta ccataaaagc
    34681 cgcccatgaa gtgttaatgg aaatgggcga gaaagacggg ataattaccc cggaacgtct
    34741 ggaaaaactg gtaaaagaaa ttttacgtca ccgtaatttc gcatcgctga gtttaccggg
    34801 tctttccgaa gagcggaaaa cagtcttcgt tcctggactg gcgattttat gtggtgtgtt
    34861 tgatgcttta gccatccgtg aactgcgcct ttctgacggg gcgttacgcg aaggcgtact
    34921 gtatgaaatg gaaggacgtt tccgtcatca ggatgtgcgt agtcgcaccg ccagcagcct
    34981 cgccaaccag tatcacatag acagcgaaca ggcccgacga gtgctggata ccactatgca
    35041 aatgtacgaa cagtggcggg aacagcaacc gaagctggcg catccgcaac tggaggcgct
    35101 actgcgatgg gccgccatgc tgcatgaggt cgggttgaat atcaaccaca gcggtttgca
    35161 tcgccactcc gcttatattc tgcaaaacag tgacttgccg ggttttaatc aggaacagca
    35221 gctgatgatg gcgacactgg tgcgctatca ccgtaaagcg attaagctcg acgatctgcc
    35281 gcgctttacc ttgtttaaga agaaacagtt cctgccactg atacagctat tgcgccttgg
    35341 cgtattacta aacaatcaac gtcaggcaac caccacaccg ccaacattga cactgattac
    35401 tgatgacagt cactggacac tgcgtttccc gcatgactgg tttagtcaga atgcgctggt
    35461 actgcttgat ctggaaaagg agcaagaata ctgggaaggc gtggctggct ggcggttgaa
    35521 aattgaagaa gaaagtaccc cagaaatcgc cgcttaatgc tttgcgggcc gacgagaaat
    35581 gtcggcccgc attattcagg cactttcgcg aatgggttcg atttcattca gcgtatcaat
    35641 taacggttgc ggcttaccaa taagataacc ctgcatataa tcgatcccca aagagagcac
    35701 cgcctcgcgg atctcttcgt tttcaacgta ctctgccact acccgcattt tcttcattcg
    35761 cgccagatgg caaatcgacg ccacaatctg ataatccaga ctatttgaca caatattgcg
    35821 gataaagctg ccgtcaattt taagcagatc ggcattcaca tttttaagcc gtgcatagct
    35881 ggcgtagccg gtgccgaaat catcaatcgc aatctggcag cctaattcct gaagatgctg
    35941 caaggtgatt tgcgcctgct taacattggt cagagcatta ctttcggtga cttcaaaaat
    36001 aagttgccac gcttcaatct gatatttagc cagcaactga ctgacttcaa caggaaaacg
    36061 agcctgacat accgaggttg gagacagatt aatagcaaaa cggtgagcgg gcatcttcgc
    36121 tctgttttca gccataaatt gcagcgtatg ctcaatgacc cacatgtcga tactcgacga
    36181 taaaccaaat tcgtgcgcga ccggcaagaa gctaccgggg ccgatcagtt catcattctc
    36241 acctttcatg cgcagaagaa tttcatggta aacatcacca cgcataccgg taatcggctg
    36301 ggccatcagg aaaaaatggt tgtgttccag cgcctgctgt agtcgattca tcatcgcgac
    36361 tttatctttc aattcgcgtt gcaaatacat tgccccgcaa cgctgcatat tttccggggc
    36421 gttggtcacg atggaaagtt cggcgaccgt atttagctct cccagcagca ggtagatatg
    36481 attcactggc gagcgcacat agcagtaact gacgccaatc tgcggttgca tcggcatccc
    36541 atcccaaaag aaacgaaatt gcttgagatg actatccagt gcggtaatgc gctcctggtg
    36601 cgattctgta ttcagtcgca gcgcgagatc gttacccgaa agctgataaa catcttcacc
    36661 cggttccagc aagggtgaca gccagtgaga aagtttttgc ttgt
//
LOCUS       DAZOYF010000020        36517 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZOYF010000020 DAZOYF010000000
VERSION     DAZOYF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.020"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..205)
                     /locus_tag="ACHUOF_000993"
     CDS             complement(<1..205)
                     /locus_tag="ACHUOF_000993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610274.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFVFIFTLKLRICGFIFNKIIT"
     gene            complement(230..2865)
                     /gene="mngB"
                     /locus_tag="ACHUOF_000994"
                     /pseudo
     CDS             complement(230..2865)
                     /gene="mngB"
                     /locus_tag="ACHUOF_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(2883..4799)
                     /gene="mngA"
                     /locus_tag="ACHUOF_000995"
     CDS             complement(2883..4799)
                     /gene="mngA"
                     /locus_tag="ACHUOF_000995"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR0610275.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            4969..5691
                     /gene="mngR"
                     /locus_tag="ACHUOF_000996"
     CDS             4969..5691
                     /gene="mngR"
                     /locus_tag="ACHUOF_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR0610276.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(5794..6663)
                     /gene="sucD"
                     /locus_tag="ACHUOF_000997"
     CDS             complement(5794..6663)
                     /gene="sucD"
                     /locus_tag="ACHUOF_000997"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR0610277.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(6663..7829)
                     /gene="sucC"
                     /locus_tag="ACHUOF_000998"
     CDS             complement(6663..7829)
                     /gene="sucC"
                     /locus_tag="ACHUOF_000998"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR0610278.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(7923..9140)
                     /gene="odhB"
                     /locus_tag="ACHUOF_000999"
     CDS             complement(7923..9140)
                     /gene="odhB"
                     /locus_tag="ACHUOF_000999"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR0610279.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(9155..11956)
                     /gene="sucA"
                     /locus_tag="ACHUOF_001000"
     CDS             complement(9155..11956)
                     /gene="sucA"
                     /locus_tag="ACHUOF_001000"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR0610280.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(12257..12973)
                     /gene="sdhB"
                     /locus_tag="ACHUOF_001001"
     CDS             complement(12257..12973)
                     /gene="sdhB"
                     /locus_tag="ACHUOF_001001"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR0610281.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(12989..14755)
                     /gene="sdhA"
                     /locus_tag="ACHUOF_001002"
     CDS             complement(12989..14755)
                     /gene="sdhA"
                     /locus_tag="ACHUOF_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR0610282.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(14755..15102)
                     /gene="sdhD"
                     /locus_tag="ACHUOF_001003"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(14755..15102)
                     /gene="sdhD"
                     /locus_tag="ACHUOF_001003"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR0610283.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(15096..15500)
                     /gene="sdhC"
                     /locus_tag="ACHUOF_001004"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(15096..15500)
                     /gene="sdhC"
                     /locus_tag="ACHUOF_001004"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0610284.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            15881..15988
                     /locus_tag="ACHUOF_001005"
     CDS             15881..15988
                     /locus_tag="ACHUOF_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610285.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16194..17477
                     /gene="gltA"
                     /locus_tag="ACHUOF_001006"
     CDS             16194..17477
                     /gene="gltA"
                     /locus_tag="ACHUOF_001006"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR0610286.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            17866..18432
                     /locus_tag="ACHUOF_001007"
     CDS             17866..18432
                     /locus_tag="ACHUOF_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0610287.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            18483..20942
                     /locus_tag="ACHUOF_001008"
     CDS             18483..20942
                     /locus_tag="ACHUOF_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0610288.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKIPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DGNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            20957..21688
                     /locus_tag="ACHUOF_001009"
     CDS             20957..21688
                     /locus_tag="ACHUOF_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0610289.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            21685..22761
                     /locus_tag="ACHUOF_001010"
                     /pseudo
     CDS             21685..22761
                     /locus_tag="ACHUOF_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            22913..23959
                     /locus_tag="ACHUOF_001011"
     CDS             22913..23959
                     /locus_tag="ACHUOF_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR0610290.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(23956..24747)
                     /gene="nei"
                     /locus_tag="ACHUOF_001012"
     CDS             complement(23956..24747)
                     /gene="nei"
                     /locus_tag="ACHUOF_001012"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR0610291.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(24783..25517)
                     /gene="pxpA"
                     /locus_tag="ACHUOF_001013"
     CDS             complement(24783..25517)
                     /gene="pxpA"
                     /locus_tag="ACHUOF_001013"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR0610292.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(25507..26439)
                     /gene="pxpC"
                     /locus_tag="ACHUOF_001014"
                     /pseudo
     CDS             complement(25507..26439)
                     /gene="pxpC"
                     /locus_tag="ACHUOF_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(26433..27089)
                     /gene="pxpB"
                     /locus_tag="ACHUOF_001015"
     CDS             complement(26433..27089)
                     /gene="pxpB"
                     /locus_tag="ACHUOF_001015"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR0610293.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(27112..27855)
                     /gene="ybgI"
                     /locus_tag="ACHUOF_001016"
     CDS             complement(27112..27855)
                     /gene="ybgI"
                     /locus_tag="ACHUOF_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR0610294.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            28126..29607
                     /gene="dtpD"
                     /locus_tag="ACHUOF_001017"
     CDS             28126..29607
                     /gene="dtpD"
                     /locus_tag="ACHUOF_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR0610295.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(29757..31175)
                     /gene="phrB"
                     /locus_tag="ACHUOF_001018"
     CDS             complement(29757..31175)
                     /gene="phrB"
                     /locus_tag="ACHUOF_001018"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR0610296.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(31172..31681)
                     /locus_tag="ACHUOF_001019"
     CDS             complement(31172..31681)
                     /locus_tag="ACHUOF_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR0610297.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            31810..32097
                     /locus_tag="ACHUOF_001020"
     CDS             31810..32097
                     /locus_tag="ACHUOF_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HFR0610298.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(32152..32532)
                     /locus_tag="ACHUOF_001021"
     CDS             complement(32152..32532)
                     /locus_tag="ACHUOF_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610299.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(32535..35921)
                     /locus_tag="ACHUOF_001022"
     CDS             complement(32535..35921)
                     /locus_tag="ACHUOF_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0610300.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDAPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(35940..36143)
                     /locus_tag="ACHUOF_001023"
                     /pseudo
     CDS             complement(35940..36143)
                     /locus_tag="ACHUOF_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT         8694 a         9973 c         8876 g         8974 t
ORIGIN      
        1 tggttattat tttgttaaaa ataaagccac atatacgtag cttgagagtg aagataaaca
       61 caaaattacc tgatgcgcta cgcttgccag tgtctacatc atctctgcaa tatattgaat
      121 ttgcctgctt ttgtagaccg aataaagcgt tcatgccgta tccggcatga acaaagcgcc
      181 ctttgtcaac aatctggccg cgcatatgtg gcttcggttt gattgctatt caggcaagcc
      241 ggtaactgaa cgtccgtgac tggccgggta aaaaaggccc cgatagattt gaaccttgat
      301 tttcctcggt ggtaatgtgt tcatccatca tcgtttctga gcaagaaatc acctcgcgac
      361 tgaaagcaac agtcgcatca caggttgctg attcagccgg attaaacagc cgtaaaatca
      421 cttcttgtcg gtcttcagct ttcttaagtg cgcttatcag gggcatccca ctgggggcat
      481 tttcaacaaa ctataacttt ccggcacgtt gaatccggct ttgttgagct tcatcgcatc
      541 ccatgggatt ttgttgtaac actgtactgg agtcagccat gctcgcgcct gctgagctac
      601 accagcggcg gttggcgtac cggtataact caataaactt aggcgacaag aaagcagacc
      661 acgtagttgt gagtccggga ctggcatttt aattcccgaa ggccgcctag gccttaaaag
      721 cagatcttct ttccccagta agcccacgcc gcgcagcaac gtaatggcaa aggttttctt
      781 ctcttcaccg atgacttcaa attcacgtag cccttcgcta aagacagcca tgccgttacg
      841 cccttcctgt aaggctgcat agttgagcat attccatacc ggaaccggcg cttctttcca
      901 gctttcttgc tgccagttgt tcattgcact gtcgttcacg gggcgcgtta gcgaaccaaa
      961 ctgggtatct gccagaacac tatcggtgtt aaaagatgta gggatcagga cgcgaaggcg
     1021 atgatcgtca gcctggttat caaggttgat atccacatca atgcgcctgt tgttatgact
     1081 aagagtgaca accaactcga catctactct gccagtggat tgtcttgcgc tgcgttgttg
     1141 cagattacgc ggcaccgcca tttcatagcg gataacagcc ctgctctgcc aggcctcatg
     1201 aataatctcg cattgcggtt tcgcattcgc cgaagtaatt acccactctt cttttgcggg
     1261 tgaatagtca tattcatcac catcatcaga gctttcttca atttgcaaca cccggtcata
     1321 gcgcacaccg ctatctttat ctaccagttg tagcgttcca tcctcattga gcgcaatttg
     1381 ccagaaagca ttttccagta tcccttcagc gtcacttttt gccgcaatca cgttgccagg
     1441 ctgattcgct tcgatataaa gcgtgcgata gcccatagaa gggacaatct ggttgatctg
     1501 tatatcaaac tccataaagg gatcgtaatt accgtaatga actatttgcc gatcgattag
     1561 gcctggatcg atctcacggg catggcgaat aaaatacggt acaggctgac cgcgatcgtc
     1621 tcgtagatta aactggctgg cgcgcagccg cacagtggtg ttgatcactt cttcccgcgg
     1681 ccagggcatc aggttaaaca ggacgagttt gtcggcgtcg ctctgcggca tgttgtcggc
     1741 gattttacgc atatagaaac gcagcagatt atccgccatg tcttcagcca gttcgaagcg
     1801 ggcgacgatt tcgcgatgaa ctttgtcacg acagcagcag ccgatgctgt cgtgggcatg
     1861 attttttaag atctctttcc acattttctc cagcaagccg tggtggtatc caaaacccaa
     1921 cgtcctggcc agcgttgcca gcggttccag cagattaaca atcttatttt caatacgtgc
     1981 gtgggcaact ttgatatcca tgcgcgtgga accgatggtg cgatgcacgc gcatatattt
     2041 gccatcaata aattcccctt tcagggttgc cagattatct cgctgcgctt cgatcttctc
     2101 aaatacctct tcaaagcggc tcatcacaaa ctttcgttga ggataaattt cgcgcagttt
     2161 ctccatcact tcgaagatat tttgctgcaa tggcatctga tcatgcccat tcggcagcaa
     2221 aatctctttg gttacagacg ctttttccag cacgtcaaaa taactgtcga ggcgtttgcg
     2281 taatccgttt tcgtccgcag gtaagtactt accgatggca taacccaacg gcatcacctg
     2341 cgccgtcact tcgctaccgt cactgctttg ccacaaaaac tcggttttat ccgtaccgtg
     2401 gcgctcagaa catccacgcc agaacatggt gcaggtaatg ccaaatccat tgtagatatg
     2461 cggcagttgc ccggacatgc caaaggaatc gggtaaataa cctattttca tcggctcgcc
     2521 aaacgcgagg cagtcacgca ttccgtacat cagattacgg acgatggatt ccgcagaaac
     2581 aatcgtggta tcggtctggg tataccaggg gccgataatc aacttgccgg cttctaccag
     2641 tttcttcaag cggtctttgt tttccggttt caccgcgaaa taatcttcga ggatcgccgt
     2701 ttgcccgtcg agtacgtaat atttgtattc gttgtcctgt tccagtcggc acaggatctc
     2761 ttccatatta ttgaccagca gaatgcgtga ctcttcggtg gtgaaatacc actcccgatc
     2821 ccagtgcata tgcggggtga tgtgaacgcg agataccgct ttcatcgtcg tttccttttt
     2881 gtttatggca ttacgccatc agtcagatag ttgccatgct taaccgcgtg acgccgccag
     2941 atcaggagaa ttgcagtcga gattgcagcc cccaccaatg ccgcaccaaa ccagccaatt
     3001 gccgccataa caccgcccgc gccattatca tgaagtaaaa agagcgagaa aatgccagca
     3061 ccgggtgtcg aaaggccgat attcatcgca ccgacaatag cgcccgttac catagagccc
     3121 agcacaaacg aaccaataac ccgcagcgga tcttcaatcg ccatcgggat cgccccttcg
     3181 gtaatacctg ccagccctaa cagccaggtg gatttcccgg tctcaatttc aaactcttta
     3241 aacaggcgcg gtgcgagcat cgtggaagcg gttacggtaa atgccgaaac cattttgacg
     3301 gaggcgaaaa tggcatacgg gccgtaaacg ccgttcgcca ttgcgcccag gcagaatgca
     3361 taagcggctt tattcactgg ccctccaagg tcaaaggaac acataaaacc gagaatggca
     3421 cccagcaaca gcgcgttact tcctgacaga ccgttcagcc aggcggtaag cgagttattg
     3481 atccaggcga caggttcccc caccacaaac agcatcagac tgcccgctcc caatgtaccg
     3541 agcaccgggt agagataaaa agtcaggaag ccattgaatt tactgctaag acgcaagtga
     3601 tttttcatcc agcgtatcaa gtaaccggct atcaatccgc caacgaccgc gccgagaaac
     3661 ccggagccga tcatgttggc ggcaagtcca gccgcaaagc ctggcgctaa cgccggttta
     3721 tctgccagcg aataggcggt ataggctgcc agcaccggta ccatcaaaat tccgagcagc
     3781 ccgccgccca gtttgcggta catccacagc caggagtttt cttcattaaa cagatcttgt
     3841 agcccgaaga tttgcgacag taataccgcg accgccagca ccgtcccccc cgcgacaatc
     3901 aacggtacgg caaaagagat cccgctcaac agtgcctgtt tcagctccgt tttgacgctt
     3961 ttcaccggtt gcgtatcttg ctgtaaggta cgcgtctcat cgccacgctc gagggtaagc
     4021 gattgttgga tcaacgcttc tgcatggcga atcggctcgg caacaggcac agaaagcgca
     4081 ggaatgccgt taaaacgctc actctccttg atggcgactt cagccgcaaa aatacaggca
     4141 gttgcactat tgagttgatc cgccgttaaa cgcccttcaa taccgttagc accttgcttt
     4201 tcaacgtaga cattcacgcc gagtttgcgt ccggcttttt ccagatactc cgcagccatg
     4261 taggtgtgag caatacccgc cgggcaggcc gttacgcaaa cgatagttgg cgcgttggaa
     4321 aaagaggcag aaggttgcgt gcctcccttg tcatccagcg ccgagagcaa ttcatcaggc
     4381 gtcgtcgccg actgtatacg cgcccgaatc tcatcatccg ccaggcgcgt ggtcagcgct
     4441 gtcagcagtt gcatgtgcgt agtacccgct tcattgaggg gaatcgccag caacaccact
     4501 aaatcaaccg cttccgggcc atccacgcct tcccactgaa gcggctcgct gagtgtggcg
     4561 actgcaaacg ccgcttcttt taccgcagca gttttgccat gcggcacagc caacccttca
     4621 cttaaggcgg tcgggccaag gctttcacgg cgatacactt ctttcagaaa ttgctcagta
     4681 ctggaaattt tccccagagc agcaagacgt tgagtcaacg cgtgaatggc ctcttcacgg
     4741 ctggtaaagc gcgcattcag acacaacgca tcgcggtggg tcagagtcgt caggttcatc
     4801 atgatcctca cttgatcgtt tttatagtcg cgccagtgtg cccgacaaaa caataccatt
     4861 gcgtaatcaa tttcacttga ttgaaattat ttgcatttta tttgtattgt ttacaggatg
     4921 agtttatcgg gcataatggc taaattcgta ttaatgagat gctaaataat gggacacaaa
     4981 cccttatacc gacagattgc tgatcgcatt cgtgagcaaa ttgcgcgtgg cgagcttaaa
     5041 cctggcgatg cgttacctac cgaatcggca ttacaaacag agtttggcgt cagccgggtt
     5101 acggtgcgtc aggctttgcg ccagttagtc gagcaacaga tcctcgaaag cattcagggc
     5161 agcgggactt acgtcaaaga agagcgggtc aattacgata tttttcagtt aaccagtttt
     5221 gatgaaaaac tgtcggaccg tcacgtcgat acacacagtg aagttctgat atttgaagtg
     5281 attccggctg acgattttct tcagcaacag ctacaaatca ccgtgcagga tcgtgtttgg
     5341 catgtgaagc gcgtgcgcta tcgcaagcaa aagccaatgg cgctggaaga aacctggatg
     5401 ccgcttgcgt tgttcccgga tctcacctgg caggtcatgg aaaattcgaa atatcacttt
     5461 attgaagaag tgaagaagat ggttatcgat cgtagcgaac aggaaatcat tcctctgatg
     5521 ccaaccgaag agatgagccg tctgcttaat atcagccaga caaagccgat tcttgaaaaa
     5581 gtatcgcgtg gatatttagt tgatggtcgg gtgtttgaat atagccgcaa cgcttttaat
     5641 accgatgact ataaatttac cctgatagcg cagcgaaaat catcgcgata agcacaaaaa
     5701 ggccatcata cgatggccaa ccatgtcgaa accgacagcc cttcgccatt cctgtagagg
     5761 gaagcggcga gggctatttc ttattacaga tatttatttc agaacagttt tcagtgcttc
     5821 accgatatcc gccaggctgc gaacggtttt cacgcctgcg gcttccagag cagcgaattt
     5881 ctcatccgca gtccctttcc caccggcaat gatcgcaccc gcgtggccca tacgtttgcc
     5941 tttcggcgca gtcacaccag cgatgtaacc cacaactggc ttggtaacat gctctttgat
     6001 gtacgctgcc gcttcttctt cagcgctacc gccgatctca ccgatcatca cgatcgcttc
     6061 ggtctgcgga tctttttcga acatttcgag aatgtcgata aagttagagc cagggatcgg
     6121 gtcaccgccg ataccgacac aggtcgactg accgaaaccg taatccgtgg tctgtttaac
     6181 cgcttcatag gtcagtgtac cggaacggga aacgataccc actttacccg gtttgtgaat
     6241 gtgacccggc tggataccaa ttttgcattc acccggggtg ataacgcctg ggcagttcgg
     6301 gccgatcata cgaacgcctg cttcgtccag tttcactttc acggtcagca tatccagcgt
     6361 cgggatgcct tcagtgatgg tgataatcag tttgatgcct gcatcgatag cttccagaat
     6421 ggagtctttg cagaacggtg ctggtacgta aataacagac gcggaagcgc cagtagcagc
     6481 aacagcttca cgcacggtgt tgaacaccgg caggccgagg tgggtagtgc cgcctttacc
     6541 tggagttacg ccaccaacca ttttagtgcc gtatgcaatg gcctgttctg agtggaaagt
     6601 cccctggcta ccggtaaagc cctggcagat aaccttggtg tttttatcaa ttaaaatgga
     6661 cattatttcc cctccactgc ggcaacaacc tgctgagctg catccgtcag accttttgct
     6721 gcaataatat tcaggccgct gtcagccagt ttcttcgcgc cgagttcggc gttgttacct
     6781 tccagacgta ccacgaccgg tacgttaaca cccacttccg ctaccgcgcc gatgataccg
     6841 tcagcgatca ggtcgcaacg aacgataccg ccgaagatgt taaccagaac ggctttcact
     6901 ttgtcgtcag agaggatgat tttgaacgct tcggttacac gttctttggt tgcgccgccg
     6961 ccaacgtcca ggaagttagc cggttcgccg ccgtgcagtt taactatgtc catcgtaccc
     7021 atcgccagac ctgcgccgtt aaccatacaa ccgatgttac cgtccagcgc aacgtagttc
     7081 agttcccact gtgcagcctg tgcttcacgc ggatcttcct gcgactggtc acgcatttcg
     7141 cgcagatcag gctggcggaa cagggcgttg ccatcagcgc ccagtttgcc gtcgaggcaa
     7201 atcagatcgc cctgtttggt gatgaccagc gggttgattt cgatcagcgc caggtcgcgc
     7261 tccaggaaaa tggtcgccag gcccatgaag attttggtga actgctgaac cagtttacct
     7321 tccagaccca gtttgaacgc cagctcgcgt ccctgatacg gcatcgggcc agtcagcgga
     7381 tcaagcgcaa ctttatggat caggtgcgga gtttcttccg ccactttttc gatttccacg
     7441 ccgccttcgg tggaggccat aaagaccacg cgacgggaac tacggtctac aacggcaccg
     7501 agatacagct ctttagcgat atcggtcgcc gcttcaacca gaatctggtt aaccggttgg
     7561 ccattggcat ctgtttgata cgttaccaga cgcttgccca gccagttttc tgcaaaagca
     7621 cggatgtctt ctttgctgtt tacaactttc acaccgcccg ctttaccgcg gccaccagcg
     7681 tgaacctgac atttcactac ccacggaccg gcaccgattt ttgaagcggc ttcttctgct
     7741 tcgcgcggag tagtacaggc ataacccacc ggtgctggta agccatagcg ggcaaacagt
     7801 tgttttgcct gatattcatg taagttcatg tgttctgtcc atccttcagt aatcgttatc
     7861 ttttaaaccg tagaccgaca ccctatccac gtcggtctac agtgcaggtg aagcttaaac
     7921 tactacacgt ccagcagcag acgcgtcggc tcttccagta actctttgat cgttaccagg
     7981 aagcccacgg attcgcgacc atcgatcaga cggtgatcgt aggacagcgc caggtacatc
     8041 atcggcagga tctcaacctg accattcacc gccatcggac gatctttgat agcgtgcata
     8101 cccagaattg cgctctgcgg cgggttgatg atcggcgtag acatcaggga accgaacaca
     8161 ccaccgttgg tgatggtgaa gttaccaccg gtcagatctt caaccgtcag cttgccatca
     8221 cggcctttga ctgccagctc tttgattttc ttctcgatgt ctgccatgcc gagggtatcg
     8281 acatcacgca gaaccggcgt caccaggccg cgcggcgtag aaaccgccat gctgacgtcg
     8341 aaatagttgt ggtaaaccac gtcatcgccg tcgatagacg cgttcacttc cgggtaacgt
     8401 ttcagggctt caaccaccgc tttcacgtag aaggacataa agcccagacg gatgccgtgg
     8461 cgtttttcaa acgcttcacc gtactgctta cgcagatcca taatcggctt catgttgact
     8521 tcgttgaacg tggtcagcat ggcggtggag tttttcgctt ccagcagacg ctctgccaca
     8581 cgcttacgca ggcgagtcat cgggacacgt ttttcactac gtgcagccag agccggttgc
     8641 gccgccggag cagccgctgc cggagcagac tctttcgccg gggctttcgc cagatgtttt
     8701 tccacatctt cacgagtcag acgaccaccc acaccggtgc ctttaatggc gctggcgtcg
     8761 agattgtgtt cagccagcag gcgacggatc gccgggctta acgcatcgtt gttttgctct
     8821 tccagagacg cctgctggcg ttgcgccgga gtggacgctt tctcttcaga tttggcgctg
     8881 gtttctttac cggcgctgtt gccttcacgc aggcgaccaa ggatctgacg agacgttacc
     8941 gttgtacctt catcttccag aaccgcatcc agaatgccgt ctgctgatgc cggtacttcc
     9001 agtaccactt tgtcagtttc gatttctacc agcacttcat cacgtacgac tgcgtcgccg
     9061 ggttttttat gccaggttgc gacggtggca tcggctacgg attcaggcag gtcagggacc
     9121 agaatatcta cgctactcat tgtgtatcct ttatttattc gacgttcagc gcgtcattaa
     9181 ccagatcttg ttgctgtttc tggtgaacgg acatataccc taccgccgga gaggcggagg
     9241 ccgggcggcc tgcataacgc agagaagccc caaacggaat cacttcacgg aaatgatgct
     9301 ggctgcagta ccatgcgccc tggttgagcg gctcttcctg gcaccagaca aaatccttga
     9361 cgtgagcaaa ctgctgcaac acttcctgca tcgctttatg cgggaacggg tagagttgct
     9421 cgatacgcac aatggcgaca tcgtgttgat tgttcttacg acgctgttcc agcaggtcgt
     9481 aataaacctt accagaacac atcactacgc gcttcacgcc cttcggatca agctcgtcga
     9541 tttcaccgat ggctggcagg aaggtgccgt tcgccagttc ttcgaggctg gaaaccgcca
     9601 gcggatgacg cagcagggat ttcggcgaca tcacgaccag cggacgacgc atcccgcgca
     9661 gcgcctgacg acgcagcatg tggtaaacct gtgccggggt agacggtacg caaacctgca
     9721 tgttttgctc agcacaaagt tgcagataac gttccagacg cgcggaggag tgctccggcc
     9781 cctgcccttc gtaaccgtgc ggcagcaaca tcaccagacc acacatccgg ccccatttct
     9841 gctcgccaga ggagatgaac tggtcgataa ccacctgcgc accgttggcg aagtcaccga
     9901 actgcgcttc ccagatggtc agggtgcgtg gttctgcggt ggcataacca tattcaaacg
     9961 ccagcacggc ttcttctgac agtacggagt cccagacacg gaacgcgccc tgaccgttat
    10021 ggatgtgttg cagcggcgtg taagtggaac cgttagactg gttgtggatc accgcgtggc
    10081 ggtggaagaa ggtaccgcga ccggagtctt cacccgacag gcgaaccgga atgccttcat
    10141 caaccagcgt ggcgtaagcg aggttttccg caccgcccca gtcgaacagt ttctcaccgg
    10201 ctgccatcgc ctggcgatcg ccataaatct tggcaacgcg agactgcatt tcaactgctt
    10261 ctggtaccgt actgatacgt ttagccagtt cctgcaggcg cttcatctca actttgttcg
    10321 ggtactcttc gtcccattcg tggttgaggt acggcgacca ggtgaaagag tgcatgttca
    10381 tcggacgcca ctctgccact acgcaatcgc cagcatccag cgcatcgcgg tacaggttaa
    10441 ccatctcggt ggcatcttcc agcgtcgcca ctttttcctg ctccagcttg tcagcgtaga
    10501 ttttgcgcgg tgtcggatgt tttttgattt tctgatacat cagtggctgg gttgcgctcg
    10561 gctcgtcggc ttcgttgtgg ccgtgacggc ggtagcatac caggtcgatg aagacatcac
    10621 gtttaaaggt gttacggaaa tcgagcgcca gacgggtcac aaaggcaacg gcttccggat
    10681 catccgcgtt aacgtggaaa atcggtgcct gaaccatctt accgatatca gtacagtacg
    10741 gtgtagaacg ggcatccagc gggttagagg tggtgaaacc aacctggttg ttgataacga
    10801 tacgtaccgt accgccaact tcataaccac gcgctttcga catgttcaga gtttcctgaa
    10861 ccacgccctg cccggtcact gcggcgtcac cgtggatggt gattggcagc actttgttgc
    10921 tgttcggctc atcaagtctg tccagacggg cacgaacaga gccgataacg accgggctta
    10981 caatctcaag gtgagacggg ttaaacgcca gcgccaggtg caccaggccg ccatcggtct
    11041 ggaagtcaga cgagaagccc atgtggtatt tcacgtcgcc tgtgccgagg tgttctttat
    11101 gtttaccggc gaactcgtcg aacaagtctt gcggtttttt acccagcacg ttcaccagca
    11161 cgttcagacg accacggtgc gccatcccga gaaccacttc gcgggtgccg ctgttgccag
    11221 cgtggcggat catctcttta agcatcggga ttaacgcgtc accgccttcc agcgagaagc
    11281 gttttgcgcc agggaatttt gcgccgaggt aacgctcaag gccttcagcg gcggtcagtt
    11341 cgcttaagaa gcgttttttc tcttcgctat tgaaagtcgc gcgaccagac tcgatacgct
    11401 gttggatcca gcgtttttct tcggtgctgg tgatgtgcat atactcggca ccaatcgggc
    11461 cgcagtaggt ttgcttgagg gcggaaataa gctcgccgag tttcatggtt tctttgccgc
    11521 tggcaaatga accgacgttg aaggtttcct ggaagtctgc ttcggtcaga tcgtggaaag
    11581 acggatccag atcggccact ttatcttgct gccacagtcc cagcggatct agattcgcat
    11641 gctggtgacc acggaagcgg tatgcgttaa tgagctgcag gactttaacc tgcttcacat
    11701 tggtgtcagg gtcggagatc gttgaagagt aacgtgaagc gtctttcgcc aggcggcgga
    11761 aatattcacg cgtttgagag tggaattgat ccggtttgac tcccgtacca ggtaactgct
    11821 ggaacgtcga acgccagtta gcgtcaaccg agtcaggatc ggttaagaag tcttcataga
    11881 gctgttctat ccagctctgg tttgcgccag agaggtaaga agagtccaac caggctttca
    11941 aagcgctgtt ctgcatcgtg atcccttaag catctttttt atgcttactt cgccgtggat
    12001 actaccacgc acagtgatgt gcgtgccggg gttcacttgc gagctcttgt ctacgtagtg
    12061 gctcgcgaag gaacctttaa aaactgtctt agtgtcaggg gcagttttta aagatttcct
    12121 gcatttcttt gttacgcctg atgcgctgcg cttatcaggc ctgcgggtaa tgacttgtag
    12181 gccggataag gcgttcacgc cgcatccggc actggttgcc tgatgcaacg cttgcgcgtc
    12241 ttatcaggcc tacagtttac gcattacgtt gcaacaacat cgacttgata tggccgatgg
    12301 cgcgcgtcgg gttcagcccc ttcggacata cactgacgca gttcatgatg ctgtgacagc
    12361 ggaatacgct gaatgcatcg ctcaaaccgt cgaggcggct gtcagtctcg gtatcacggc
    12421 tatcgatcag gaaacgatat gccgctaaca agcctgccgg gccgataaac ttatcgggat
    12481 tccaccagaa agacggacaa gaggttgaac aacatgcgca gagaatacat tcatacagcc
    12541 cgtcgagttt ttcgcgctgc tctggcatct gtaaatgctc gcgagctggc ggattttgtc
    12601 cattattcaa caggtaaggc ttaattttct catattgcgc atagaattgt cccatgtcta
    12661 ccaccaaatc gcggatcacc ggtaaacctg gcagcgggcg aatcacaatc ttcttgcccg
    12721 gctggttgag tgccgaaatc ggggtaatac aggccagacc attcttgccg ttcatgttca
    12781 gaccgtcgga accgcacaca ccttcacggc aggagcggcg gaacgacagg ctgggatctt
    12841 tctctttcag ctggataagc gcatccagca gcatcatgtc gcgaccttct tccgcttcca
    12901 gggtgtaatc ctgcatacgc ggagcatcat caacatccgg gttatagcga taaattgaaa
    12961 actcgagtct cattttcctg tctccgcatt agtaagtacg aatcttcggc gggaatgccg
    13021 ggcgcagttt cggttccatg ttgacgcttc ggcgcgtcat ggattccgac tcaggcagat
    13081 acagggagtg gcacagccag ttttcatcat cacgatccgg gaagtcgaag cggctatgtg
    13141 cgccacggct ttcggtacgg aagttggcag aaacagccgt tgcatacgcc gtttccatca
    13201 ggttatccag ttccaggcac tcaacgcgct gggtgttgaa ctcgctggaa gtgtcatcca
    13261 gacgggcatt tttcagacgc tcgcggatca ctttcaactg ctcaagccct ttcgccatcg
    13321 catcaccttc acggaagacc gagaagttat gctgcataca ttcttgcagc gctttacgga
    13381 tcgccaccgg atcttcaccg ttacgattat tgttccaccg gttcaggcga tccagagacg
    13441 cttcaacatc agactcgctg gcatcgtgca gtgcgccctg ctcggcgata gactcttgca
    13501 gatgcagacc tgccgcgcga ccaaagacca ccaggtccag cagcgagttg ccgcccagac
    13561 ggttagcgcc gtgtaccgat acacaagcga tttcaccaac ggcaaacagt cccggaacaa
    13621 ccacatcttc gcctttctca ttcacagtca gcgcctgacc ggtaactttg gtcggaatac
    13681 cgcccatcat gtagtgacag gttgggataa ccggaatcgg ctctttcacc ggatcgacgt
    13741 gagcgaaggt acgggaaagc tccaggatac ccggcagacg ggattcgaga acttctttac
    13801 ccaggtgatc gagtttcagt ttcgcgtgtg gcccccacgg accatcacag ccgcgacctt
    13861 cacggatttc aatcatgatg gaacgcgcca ccacatcacg gcccgccagg tctttggcgt
    13921 tcggcgcata acgctccata aagcgttcgc catgtttgtt cagcagataa ccgccttcac
    13981 cacggcaacc ttcggtgacc agtacgcccg caccggcaat gccggtcggg tggaattgcc
    14041 acatttccat atcctgcacc ggtacgcctg cgcggatagc catgccgaca ccgtcgccag
    14101 tgttaatgtg ggcgttagtg gtggactgat aaatacgccc tgctccgcca gtcgccagca
    14161 cggtagcgcg ggctttgaaa taaaccactt caccggtttc gatgcacagt gcggtacaac
    14221 ccaccaccgc gccatcctgg tttttcacca gatccagcgc ataccactcg gagaaaatgg
    14281 tggtgtggtt tttcaggttc tgctgataaa gcgtgtgcaa cagtgcgtga ccggtacggt
    14341 cagcagccgc cgcagtgcgt gccgcctgct cgccgccgaa gtttttcgac tgaccgccaa
    14401 acggacgttg atagatacga ccatcatcga gacgcgagaa cggcaggccc atgtgttcga
    14461 gttccagaat cgcttccggc ccggttttac acatatattc aatcgcgtcc tggtcaccga
    14521 tatagtccga cccttttacg gtgtcgtaca tatgccattc ccagttatct tcatgggtat
    14581 tacccagcgc aacggtaatg ccgccttgcg cagaaacggt atgggaacgg gtcgggaaga
    14641 ctttagagag cagggcacag gtctggccgc tctgggaaat ttgcagcgcc gcgcgcatac
    14701 ctgcgccacc ggcaccaatc acaactgcat caaattctct gactggcaat ttcatcacac
    14761 accccacacc acaacgaatc cataaatcac gtaaaccacc agtgcaacga caatcaccag
    14821 ttgcagcatc aggcgcaagg ccagcggttt aacgtagtcg gtcaacacct gccacatgcc
    14881 gatccaggca tgaatcaaga tagaaaacag cgccagcagg gtgaacactt tggtgaacgc
    14941 agaggcgaag aaaccgatcc agacttcata tgtcagttcg ccactggtgg cgaaaaaacc
    15001 gaccatataa atgatgtaga gcgtcaggac gatagcggta gcgcgaacga ggatgaaatc
    15061 atgtacgcca ttgcgtccta atgcggaggc gttgcttacc atacgaggac tcctgcgaga
    15121 agtgaaagca cgacagtaat aacaaaggag attttggcgg agcgtttacc cgcttcgaat
    15181 gtttcttcca gatagccaaa atccatcatc atgtggcgaa tacctacgac gacgtgatac
    15241 gccagagcgg taaggatgcc ccacatgata aatttgacga agaagctgcc cataatcgcg
    15301 gaagcttgct cgaaaccttc aggggaagag aggctggtac ccagaagcca ctgcaggatg
    15361 cccactgcaa caaaggtgat cacaccggaa acgcgatgga gaatggacgc tatcgccgtg
    15421 atggggaacc ggatggtctg taggtccaga ttaacaggtc tttgtttttt cacatttctt
    15481 atcatgaata acgcccacat gctgttctta ttattccctg gggactacgg gcacagaggt
    15541 taactttctg ttacctggag acgtcgggat ttccttcctc cggtctgctt gcgggtcaga
    15601 cagcgtcctt tccataactg cgcgtcatgc aaaacactgc ttccagatgc gaaaacgaca
    15661 cgttacaacg ctgggtggct cgggattgca gggtgttccg gagacctggc ggcagtatag
    15721 gctgttcaca aaatcattac aattaaccta catatagttt gtcgggtttt atcctgaaca
    15781 gtgatccagg tcacgataac aacatttatt taatttttaa tcatctaatt tgacaatcat
    15841 tcaacaaagt tgttacaaac attaccagga aaagcatata atgcgtaaaa gttatgaagt
    15901 cggtatttca cctaagatta acttatgtaa cagtgtggaa gtattgacca attcattcgg
    15961 gacagttatt agtggtagac aagtttaata attcggattg ctaagtactt gattcgccat
    16021 ttattcgtca tcaatggatt ctttacctgc aagcgcccag agctctgtac ccaggttttc
    16081 ccctctttca cagagcggcg agccaaataa caaacgggta aagccaggtt gatgtgcgaa
    16141 ggcaaattta agttccggca gtcttacgca ataaggcgct aaggagacct taaatggctg
    16201 atacaaaagc aaaactcaac ctcaacgggg acacagctgt tgaactggat gtgctgaaag
    16261 gcacgctggg tcaagatgtt attgatatcc gtactctcgg ttcaaaaggt gtgttcacct
    16321 ttgacccagg cttcacttca accgcatcct gcgaatctaa aattactttt attgatggtg
    16381 atgaaggtat tttgctgcac cgcggtttcc cgatcgatca gctggcgacc gattctaact
    16441 acctagaagt ttgttacatc ctgctgaatg gtgaaaaacc gactcaggaa cagtatgacg
    16501 aatttaaaac tacggtgacc cgtcatacca tgatccacga gcagattacc cgtctgttcc
    16561 acgctttccg tcgcgactca catccaatgg cagtcatgtg tggtattacc ggcgcgctgg
    16621 cggcgttcta tcacgactcg ctggatgtta acaatcctcg tcatcgtgaa attgccgcgt
    16681 tccgcctgct gtcgaaaatg ccgaccatgg ccgcgatgtg ttacaagtat tccattggtc
    16741 agccatttgt ttatccgcgc aacgatctct cctatgccgg taacttcctg aatatgatgt
    16801 tctccacgcc gtgcgaaccg tatgaagtta atccgattct ggaacgtgct atggaccgta
    16861 ttctgatcct gcacgctgac catgaacaga acgcctctac ctccaccgtg cgtaccgctg
    16921 gctcttcggg ggcgaacccg tttgcctgta tcgcagcagg tattgcttca ctgtggggac
    16981 ctgcgcacgg tggtgctaac gaagcggcgc tgaaaatgct ggaagaaatt agctccgtta
    17041 aacacattcc ggaatttgtt cgtcgtgcga aagataaaaa tgattctttc cgcctgatgg
    17101 gcttcggtca ccgcgtgtac aaaaattacg acccgcgcgc caccgtaatg cgtgaaacct
    17161 gccatgaagt tctgaaagag ctgggcacca aagatgacct gctggaagtg gctatggagc
    17221 tggaaaacat cgcgctgaac gacccgtact ttatcgagaa gaaactgtac ccgaacgtcg
    17281 atttctactc tggtatcatc ctgaaagcga tgggtattcc gtcttccatg ttcaccgtca
    17341 ttttcgcaat ggcacgtacc gttggctgga tcgcccactg gagcgaaatg cacagttacg
    17401 gtatgaagat tgcccgtccg cgtcagctgt atacaggata tgaaaaacgc gactttaaaa
    17461 gcgatatcaa gcgttaatgg ttgattgcta agttgtaaat attttaaccc gccgttcaaa
    17521 tggcgggtta atttttatat gcctaaacac aaaaaattgt aaaaataaaa tccactatcg
    17581 gacattcata tattctaaaa aaaaacatcg gtataaatta taaacaaata gatgattttt
    17641 aatcatctat cttcctctca gttacatgaa taaaccacca aatatagcgc acccctataa
    17701 ttcccaatac tttattcatc aaacatttaa ttcataccat caattaatta cttttacatt
    17761 taataaaatt aataatattt ttaagaaaaa ctaaaaacat tcgacaatca aataagatat
    17821 tcttgcaccg atatactttt ttcttttctg ttaaaggact cagttatgtt taaaggacaa
    17881 aaaacattgg ccgcactggc cgtatctctg ctgttcacag cacctgttta tgcggctgat
    17941 gaaggatccg gtgaaattca ctttaaaggt gaagttattg aagcaccgtg tgaaatacat
    18001 caggatgata ttgataaaga ggttgaactc ggtcaggtga ccaccagcca cattaatcag
    18061 tcacatcaca gcgatgccgt tgctgtcgac ctgctcttag tcaactgtga tctggaaaac
    18121 tccagcaacg gttccggtgg caagatttct aaagttgcag tcacctttga tagctcagcg
    18181 aaaaccaccg gcgcagatcc gattctcaac aacaccagca cgggtgaagc caccggcgtc
    18241 ggcgtacgtt taatgaataa agatcaaagc aatatcgttt taggcaccgc tactccagat
    18301 atcgacctgg ctccgacctc cagcgaacaa acgctgaatt tctttgcctg gatggaacaa
    18361 attgatcagg ctacacctgt aacgccaggc gcagttacgg cgaatgcaac ctacgtgctg
    18421 gattataaat aagtattact ttggcggcgc tatcgccgcc tttcataaaa tatttatcag
    18481 gtatggacac cgtgaatatt tatcgactct cttttgtatc ctgcctggtc gtggcgatgc
    18541 cttgcgcatt ggcggtcgaa ttcaatctga atgttctcga taaatcaatg cgcgaccgca
    18601 ttgatatttc attattaaag gaaaaaggag tcattgctcc cggtgagtat tttgttagcg
    18661 ttgcggtaaa taataaccaa atcagtaacg agcaaaagat taactggcac aaaaatgacg
    18721 ataaaaccat tccgtgcatc aatgatttac tggtcgataa atttggctta aaacctgaag
    18781 tccgtcagtc gttaccattg ataaatcagt gcgtcgattt tagctcccga cctgaaatgc
    18841 tcttcaattt cgatcaagcc aatcagcaac tgaatattag tattccgcaa gcctggctgg
    18901 cgtggcactc agaaaactgg accccaccct ccacatggaa agaaggtgtc gccggtgtcc
    18961 tgatggatta caacttgttt gccagcagct accgcccaca ggatggcagc agcagcacta
    19021 atcttaacgc ctatggaaca gccggaatca acacgggggc atggcgctta cgcagtgatt
    19081 atcagttgaa tcatactgat agcgatgata accatgaaca gtcaggcgaa atatcacgta
    19141 cctatctttt tcgcccgtta ccgcaattag gttcaaaatt aaccctcggc gaaacggatt
    19201 ttagtcccaa tattttcgac ggtttttctt ataccggcgc ggcactggca agtgacgatc
    19261 gaatgttgcc atgggaacta cgcggctacg ccccacaaat tagcggtatt gcacagacca
    19321 atgccacggt gacgatcagt caatcaggcc gcgtcattta ccagaaaaaa atcccaccag
    19381 gcccatttat cattgacgac cttaatcagt ctgttcaggg cacactggat gtcaaagtga
    19441 cggaagaaga tggtcgggtg aacaatttcc aggtttcagc agcatcgacg cccttcctga
    19501 ctcgtcaggg acaggttcgc tataaactgg ccgcgggtca gccacggccc tccatgtcac
    19561 atcaaactga aaatgaaacc ttttttagca atgaagtttc ctgggggatg ctgtcaaaca
    19621 cctcgctgta cagcggcctg ctgctttctg gtgatgacta ccattctgcc gcaatgggta
    19681 ttgggcaaaa tatgctgtgg cttggtgcgc tgtcgtttga tgtcacgtgg gccagtagcc
    19741 attttgatac tcagcaggac gagcggggct taagctaccg ttttaattac agcaaacaag
    19801 tggatgccac taacagcacg atttccctcg ccgcttatcg tttctccgat cgtcattttc
    19861 acagctacgc caactatctg gatcacaaat acaacgacag cgatgcgcag gacgaaaaac
    19921 agacgatcag cttatctgtg ggccaaccga ttaccccact aaacctcaat ctttacgcca
    19981 acctgctaca tcaaacctgg tggaatgcag acgcctccac gaccgccaac atcacggcag
    20041 gttttaatgt tgatattggt gactggagag atatctctat ttctacgtca ttcaatacaa
    20101 cccattacga agataaagat cgcgacaacc agatttacct gtcgatttcg ctccccttcg
    20161 gtaacggtgg tcgggttggc tatgacatgc aaaacagtag ccacagcacc acacaccgca
    20221 tgtcgtggaa cgatacgctg gatgaacgta atagctgggg catgtctgcc ggactgcaat
    20281 ccgaccgtcc tgacaatgga gcccaggtga gcggtaacta tcagcacctg agttcagcgg
    20341 gtgagtggga tatttctggt acctatgccg ccaatgatta cagttccgtc agcagcagct
    20401 ggagcggttc tttcaccgca acccaatatg gtgcagcgtt tcatcgccgc agctccacca
    20461 atgaaccacg cctgatggtc agcaccgatg gcgtggcaga tattccggtt cagggcaatc
    20521 tcgactacac caaccatttt ggcattgcgg tggtgccgtt gatttccagt taccagcctt
    20581 ccaccgtggc ggtgaacatg aatgacttac ccgacggcgt aacagttgca gaaaacgtta
    20641 tcaaagaaac gtggattgaa ggcgcgatag gttacaaatc actggcttcc cgttccggta
    20701 aagacggtaa cgtcatcatt cgcaacgcca gcggtcagtt tcctcccctc ggagcggata
    20761 tccgccagga tgacagcggc attagcgtgg ggatggttgg cgaggaagga catgcctggt
    20821 taagcggagt cgctgaaaat caaaagttta ccgtggtctg gggtgatagc cagcattgct
    20881 cgctccatct tcctgaacat atggaagaca ccgcaaatcg cctgatttta ccttgtcatt
    20941 aattaaggaa atgaaaatga catttatgaa aggactaccc cttttgttgt tggttgccag
    21001 tctgtgcagc cacgctgcac tacaacccga tcgcactcgt attgtgttta acgctaatga
    21061 taaagctacc agtctgcggg tggataatcg tagcgataaa ctcccctacc tcgcttattc
    21121 ctggctcgaa aatgaaaaag gggagaaaag cgatgatctt ctggtcgccc tgccgccgat
    21181 acaacgtctg gaaccgaaag caacaacaca ggtacggata gtaaagcaag cctcgaccac
    21241 aaaattaccg ggcgatcgcg aaacgctatt tttctacaat atgcgtgaaa ttcctcccgc
    21301 cccggaaaaa aacagcgacc atgccgttct gcaagtcgcc attcagagtc gtatcaaggt
    21361 gttctggcga ccggctgcat tacgcaagaa agcaggagaa aaggtcgaac tgcagttaca
    21421 ggtcagccag cagggcaacc aactgacact gaaaaatcct accgcgtatt atctgaccat
    21481 tgcctacctg ggacgaaatg aaaaaggcgt tctccccggc ttcaaaaccg tgatggttgc
    21541 gccttttagt acggtcaaca caaacaccgg aaattatagc ggcagccagt tttatctcgg
    21601 ttatatggat gattacggcg cgttacgcat gacgacgctc aactgcagcg gacaatgcca
    21661 tttacaagcc gtggaggcga agaaatgagt gctggcaagg gagtgttgct cgtcatctgt
    21721 ctgttatttc tgtcgttgaa gtctgcgctt gccctgaact gctactttgg ttcatccggt
    21781 ggctcggtag aaaaatcaga agcgattcaa ccttttgctg taccaggcaa tgccaaactc
    21841 ggcgataaga tctgggaatc tgacgatatt aaaataagat ctgggaatct gacgatatta
    21901 aaattcccgt ctattgtgac aacaatacca acggcaactt tgaaagcgaa catgtctacg
    21961 tatgggtaaa cccctacccg ggggtgcagg atcgttatta tcaactcgga gtaacttaca
    22021 atggcgttga ttatgacgcc aaccagggaa aaagccgcat cgacactaac cagtgtatcg
    22081 acagtaagaa cattgacatt tatacccctg atgggatggc agaacaaaat ttgctcgggt
    22141 gatcccgcta atattcacat gtcgcgaacc tttctcgctc gcatgcggtt atacgtcaaa
    22201 atacgagaaa tgccgccgca tgattatcaa agtacgctta gcgactatat cgtcgtgcaa
    22261 tttgacggtg ccggtagcgt taacgaagac cctactgccc aaaacctgaa atatcatatt
    22321 actggtctgg aaaacattcg cgtgctggat tgcagcgtca atttttccat ttcaccggaa
    22381 acacagatga ttgattttgg taaatttaat ttgctggata ttcgtcgcca cacaatgtcg
    22441 aaaacattca gtattaagac gacaaaatca caaaatgacc aatgcaccga cggatttaaa
    22501 gtcagttcct ccttttatac cgaagaaacg ttggttgaag aagataaagc attgttgatt
    22561 ggtaatggtc tgaagctacg tttgctggat gaaaacgcct cgccttatac cttcaataaa
    22621 tatgctgaat acgccgattt caccagcgac atgttggtct acgaaaaaac ctatacagct
    22681 gaactttcgt ccattgcagg tacccccatc gaggctgggc ccttcgatac agtggtgctt
    22741 tttaaaatta actataactg atttgccatg atgccctttg cagccggaaa gggcatctct
    22801 ttcagaaaat tgctgatttt ccccttttcc ttgattgctg ttattttttc ctagcccggc
    22861 tttctttatg ctgtatcgat acaccaaaga gaaaataatg agggagcgaa ggatgccagt
    22921 tttgcagtgg ggaatgttat gtgtgctatc acttctcctt tctattggtt tcctcgcggt
    22981 acatctcccg gcggcgctat tacttgggcc gatgatcgcc gggatcatct tcagtatgcg
    23041 cggaattact ctgcaacttc cccgctccgc ttttctcgcc gcgcaggcca ttcttggctg
    23101 catgattgcg caaaacctca ctggttcgat tctcaccacg ctggccgcca actggccgat
    23161 cgtgcttgct attttactgg tgacgctgct ttccagcgcc atcgtgggtt ggttattggt
    23221 gcgctatagc tcactgcctg gaaataccgg tgcctggggt tcttctccag gcggcgcagc
    23281 ggcaatggtc gctatggcgc aagattacgg cgcagatatt cgcctggtgg cgtttatgca
    23341 atatctgcga gtgctgttcg tcgcgggggc cgctgttctg gtcacccgca tgatgctggg
    23401 tgataacgct gaagcggtta accagcaaat cgtctggttc ccgccagtga gcattaatct
    23461 cctgcttacg attttgctcg cagtcgttgc cggtacggcg ggatgcatgc tgcgtcttcc
    23521 ttccggcacg atgctcatcc cgatgctggc gggcgcagtg ctccagtctg gtcagctcat
    23581 caccatcgaa ctaccggaat ggctgctggc gatggcgtat atggcaattg gctggcggat
    23641 tggtcttggt ttcgataaac aaatcttact gcgggcacta cgcccgctac cgcaaatcct
    23701 gctgtcgatt tttgctctgc tggctatttg tgcaggtatg gcgtggggac tgacccggtt
    23761 tatgcatatt gattttatga ctgcctacct cgccaccagt cccggcgggc ttgataccgt
    23821 ggcggtcatc gccgcaggga gcaatgccga tatggcgctt attatggcga tgcaaaccct
    23881 gcgcctgttc agtattttgc tgacggggcc agccattgcg cggtttattt caacctatgc
    23941 gccgaagcgg tcggcctagt gctggcagcc tggacaccag taaaacgggc gcgaggacaa
    24001 cgtggttttc tcaatgatgc cgccacagcg ttcgcacagt tcgccatcac gatgaaaaac
    24061 cttaaagcga aacagcgccc catgatgctt attctcatcc acctgccccc gcgtagcgta
    24121 agaaaaccga ggaatctcca gtaacgcgtg tgcgagtgca tccagttgcg ccgcattgag
    24181 atctttcgct ttatgatttc cagtcaaccc aacctgccag aggatctcca cccgcaaata
    24241 attgccaagc ccagccagaa acgcctgatc gagcagtaat cctgcaaact gacggttacg
    24301 aaagcgcggc gacaataacc gttcctttac cacctccggc gtcagattcg gatccagcac
    24361 atcgggacca acgcgttgta aaaacggatg cgtggtcagt tgttccggcc tcaacatctc
    24421 aatatccgag gcgctataaa gcagaatggt tttgtcagcc gtttgcagtt ttacccgcaa
    24481 tactcgcgtg gtctgcggct cttcgccggt atcaaccacg cgccagacgc cgtaaagctg
    24541 attatggctg tagagcgtta aaccgttggg aaaatgagtt aacaacgcct taccacgcgt
    24601 ttccacatgg gtaacgtgtt gaccgataag ttgtgattga taagttttta actgcgggaa
    24661 ggcaaaccag acatcagtta gtggtttgcc tttgatcgcc gcctccaggt tatccgctgc
    24721 acggcggatc tccgggcctt caggcatggt gatatccttt tttcattctt tattaagcgg
    24781 ggttatgctg cgacaacaat cccctttttg gcaaatgtag ctcgtaatcg gcgggcgaac
    24841 gccagagcgt gctcgccgtc gccatgcagg cagaccgttt gcgccgcgac cgttgcccat
    24901 tcgccggtga tacttttgac tctgccgtgt tgcaccatct ccagcgtttg cgccaacgcc
    24961 tgttcttcgt tttcaatcaa cgcgcctggc tgacttcgcg gcaccagcga gccgtcagcc
    25021 tgataaccgc gatcggcaaa cacttcctcg cgcgttgtca ggccatattg cttgcctgca
    25081 cgaatcagct cgcttcctgc cagcccaacg agaaccaatg ccggatcgca agcgtatacc
    25141 gctctggcga tggcgtctgc cagttgtgct tctttcgccg cctggttgta caacatgccg
    25201 tgcggtttga catgacgcat tacgccgcct tgcgcacggg caatcgttgc cagcgcgcca
    25261 atttgataca gcgtctgggc gtaaacggtt tccggcggca gctgcatggc gctgcgacca
    25321 aaattttccc tgtcgggaaa actcgggtga gcgccaatcg cgacaccatt ttttattgct
    25381 tcacgcacgc aagcctgcat ggtttgcgca tcgcctgcat gaaatccaca agcaatattg
    25441 gcagaggaaa ccagcgttaa tagctctgcg tcgctggcgc agccttcgcc cagatcggcg
    25501 ttcaggtcaa ttttcattgt gcagccgcca cgctaattgt tcaaaataac gttgctgatc
    25561 ttgccgcgct ttcagtgcct cttccagtga acactggaca aaatgaatcg gctgaccgag
    25621 cggaatttgc gccagatggt acatatcagc ctcaatgata caggcaatac gtggataacc
    25681 accggtggtc tgtgcgtcgt tcatcaacac aatcggctgc ccgttatgcg gcacctgcac
    25741 cacgcccggt aacaaaccgt gagataacag ttcgcgatcg gtgatgcgtt ttaaaatttg
    25801 cccctgtaaa cgatagccca ttcggttact ttgcgagcta agctgccagg gcgaacgcca
    25861 gaatgcatcc tgcgaggcgc gatcgaactc atgatattcc ggccccggca aggcgcgaat
    25921 gcggttgccc cacagcagct gtttaacgcc ctgcgcttcc ataaaatcac gcttcgattt
    25981 gccaatcggg agtcagtcac catccttcag taaacggcct tccagcccgc caatccccac
    26041 tttgagatcg gtgctgcatg accccattac cggcggaaca tcaataccac ccgcgaccgc
    26101 cagataactg cgcatcccgt gctgcgggcg tttaagcgtt aaacgctggc ctgctttcat
    26161 cggcaatcgc cagccggtcc agacggcgtt atcatccaac cgcgcttcgc aacctgcacc
    26221 cgtcagggca aaccacccat cagtttcgaa ctcaactgtt aactgaccga gcgtgatctc
    26281 cagcgcgggg gcattggcgt cattacccac cagcaggtta gcaatgcgca gcgcaggcat
    26341 atccagtgcg ccacagtggc tgatacccga ctggcaaaaa ccgtgacgac cgccatcctg
    26401 cacagtggtg tacatgcccg cacgaataat cttcagcata ttccctcctt ctgcggtaca
    26461 aatcgcacgc tgtctcccgg acgtaataag atgggttcgt cacgcgccgg atcaaacagg
    26521 ctgagtgagg tatgaccaat caactgccag ccacccggcg ttgccagcgg ataaacacca
    26581 gtctgcggcc cgccgatccc gacagaacct gccggaacga gcaagcgcgg ttcagcgcgc
    26641 cgtggcgtgt gtagttgttc cggcaaactc ccgagatacg ggaagcccgg ttgaaaacct
    26701 aaaaaccaga ccacgtattc cacggaggag tgcaattcaa caacctgttt ttcgctcaac
    26761 ccgcaatacg ccgcgaccac cgccaaatcc ggtccgcctg caccaccgta aaccaccgga
    26821 atttcaataa agcgagactc aggttccagg gcctcgctct cctcccacca gcgttgcaaa
    26881 cgctctatgg catccagcgc cagcgactca ggattacgca aaatcaccgt gatattgttc
    26941 atgccgggaa tggcttcaac cacattcggc atatccacca gacgctgcgc cagtcgccag
    27001 atccgtttct ggctagccag cgtcaccggc ggttccagtt ccagcactac cgccgtttca
    27061 cctatcagat aacaacgcgc tcgttgcact ttcgatccct ctgattattc gttatgcagg
    27121 attaggaata tcaataaagg tcacatcaag atcggtattt tcattcagcc actcgctcaa
    27181 tgcgcgaata ccaccgcgtt cagtggcatg gtgacctgca gcataaaaat gcaatccctg
    27241 ctcgcgggct gaatgaatgg tctgttcaga aacttcgcca gtaataaaag catccacgcc
    27301 aaaacgcgcg gcgctatcga taaaactttg cccgccgccc gtgcaccagg cgacgcgctg
    27361 gaccacctca ggtccggtat cgccacacca taacggctta cgtcccagac gcgcttcaat
    27421 ccaggaggct agttccattc ccggcacagg catggtcagc tctccccacg gcaccaacgg
    27481 ctcaatttcg cccatgaccg tgatccccag taacgccgcc agctgtgcgt tattgcccag
    27541 ctcaggatgt gcgtcaagcg gcaaatgcca gccatacagg ttgatatcat tcgccagcaa
    27601 cgtttttaaa cggttacgct tcattccgcg aatgaccgga gactctcctt tccagaagta
    27661 gccatgatgc acaatgaccg catccgcgcc cagacgcact gcctcatcga gcaaagcctg
    27721 gctggcggtg acaccggtaa caattttttg caccgtctct ttgccttcca cctgcaaacc
    27781 attcggcgcg taatcactaa tcgccgcgct gttcagtttt tcgttaatca gttgttccag
    27841 ttcggtgttt ttcatcttcg cgccttccgt attctgatag ctacaccgtt aacatagcgt
    27901 tacatcgcct gttcgtcgat agccaatctt ttgacatatt aagaaacaaa cccgacgcca
    27961 aaagacgcaa attgtttata tcatcacgct tcagctaatg tatatttatg caatcagaca
    28021 gcatattgca taaataatca gatctgtatc aaagttttaa ctttaaggaa acacaagcac
    28081 gccgcgcaac tattacgtga cgttgccgtc aattgtggaa ttattatgaa taaacacgca
    28141 tcacaaccgc gcgctattta ctatgtcgtt gcgctgcaaa tctgggaata ttttagcttt
    28201 tacggcatgc gtgccctgct gattctctat ctcaccaatc aactaaaata caacgatact
    28261 cacgcctacg agttatttag cgcctactgt tcgctggtgt atgtcacgcc aatcctcggt
    28321 ggctttttgg cggataaagt tctcggcaat cgcatggcgg tgatgctggg ggcgttgttg
    28381 atggcgatcg gtcatgtggt gctgggtgcc agtgagatcc atccgtcatt cctctatctg
    28441 tccctggcga ttatcgtctg cggctatggc ctgtttaaat ctaacgtaag ctgtctgctc
    28501 ggcgagctgt atgagccaac cgatccgcgt cgtgatggcg gtttctcgct gatgtatgcg
    28561 gcgggtaacg tggggtctat tatcgcacct atcgcctgtg gttacgccca ggaagagtac
    28621 agctgggcga tgggctttgg cctggcggcg gttggaatga tcgcgggtct ggtcattttc
    28681 ttatgtggca atcgtcattt cactcatacc cgcggcgtta acaaaaaagt actgcgtgcg
    28741 acaaactttc tcctgccgaa ctggggatgg ctgctggttc tgctggtggc aacgcctgca
    28801 ctgattaccg tgctgttctg gaaagagtgg tcggtatacg ccttaattgt cgcgactatt
    28861 attggcctgg gtgtactggc aaaaatttat cgcaaagcag aaaaccagaa acagcggaag
    28921 gagctggggc tgattgtgac gctcaccttc ttcagtatgt tgttctgggc cttcgcacaa
    28981 cagggcggta gctcgattag cctttatatc gaccgcttcg ttaaccgcga tatgtttggt
    29041 tataccgttc cgaccgcgat gttccagtcg attaatgcct tcgcagttat gctgtgcggt
    29101 gtgttcctgg cgtgggtggt taaagaaagc gtcgcaggta atcgtaccgt gcgcatctgg
    29161 ggaaaatttg ctcttggcct tggcctgatg agcgccggat tctgcattct gaccttaagc
    29221 gcccgctggt ccgcaatgta tggtcactct tctctgccac tgatggtgtt aggcctcgcg
    29281 gtgatgggct ttgcggaact gtttatcgac ccggttgcca tgtcgcaaat tacgcgcatt
    29341 gaaattccgg gcgtgaccgg cgtattaacc ggcatctaca tgctgctttc tggcgcgatc
    29401 gcgaactatc tggcgggcgt gattgccgat cagacatcgc aggcttcgtt tgatgcttcc
    29461 ggggcgatca actactccat caatgcatat attgaagtat ttgatcaaat tacctggggc
    29521 gcactggcgt gtgtaggact ggtactgatg atttggctgt atcaggcgct gaaattcaga
    29581 aaccgcgcgc tggcgctgga gtcataatag gccagcaccg atgcctgatg cgacgcttga
    29641 cgcgtcttat caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc
    29701 tggattaggc gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat
    29761 ttccccttcc gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg
    29821 actatcggtt gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca
    29881 tgcaccgctt tccctggtac atcgcgcagc tccggtaacc atcgacgaat aaactcgccc
    29941 tcacggtcaa atttctcgcc ctgagttatc ggattgaaaa tacgaaaata cggcgcagca
    30001 tcagtacccg tagaagccgc ccactgccag ccaccgttat tagctgccaa atcaccatca
    30061 atcagctgcg acatgaaata tcgctcgcct tcgcgccagt cgatcaacaa atctttcacc
    30121 agaaaactgg ctgtaatcat ccgtagccgg ttatgcatcc agccagtgct gttaagctga
    30181 cgcatagcgg catcaataat cgggtatccc gttttgcctt tctgccaggc ctgtaaatgt
    30241 gcgggattgc tttgccactg cacacgatcc gtccaggcaa taaatggacg atgtttacac
    30301 aacgaggggt aatacgtcat cagatgacgg tagaactcgc gccagatcag ctcactaagc
    30361 cagacactac cggccccacc gtccagcgcc tgcggctgtt ccgccaacaa gcgatgcaaa
    30421 cactggcgag gcgataaccc gcccgttgcc aagctggcgg acaaacggct ggtgccttcc
    30481 actgccggaa aatctcgttg ttgctcatac tctccagcac cgttctcgca aaactggcgt
    30541 aattgcgcaa tcgccgcttt ttcttccacc gggaaatgcg cagtatcgaa agactgacga
    30601 ggataattca gcgtaatgga tggcgcgggc tttatcgatc cgctactacg aacttttggt
    30661 gcagcgacgc actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc
    30721 gtaaagactt tgtacatctc gtgattgccg gtcatcaccg cccccggcgg caggatcacg
    30781 ctgtcatcaa atccttcaca caccacgtta cgcagcgctc tttcaacgtc cacatcccgc
    30841 gctcgctcat tcacctcata ctgatagtta taaaaaagat gagtaacgct gttttccgcg
    30901 cacacctgtt taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa
    30961 ggaatacctt tttccgccag cgcgatttgt agcccattca gttgagcatt gataagttca
    31021 gcctgacgcg gcgacatgtt atgcgtctcc cacttgcgtg gtgtagcgat atacagcgcc
    31081 agcacgcgtg cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt
    31141 aaatcctggc gaaaccaggc cagatgggta gtcataaaac tcctgaatgg cgcaagttaa
    31201 tcacacgcgg ccaaagttcg aaataacgct gccctgaaag ccaggcgtca ggataaagcg
    31261 ccatatagtg tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac
    31321 tgtcgataag cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac
    31381 cctgaacgtg catcaaaacg ttggtgtgat cgcgcgggtt ggcgggatga gaaagcaaaa
    31441 caattacgcg ttggcggtac tggttgtaaa aatcatccag attttgccac tgatgaatat
    31501 cagccacaaa cgggccaagt ttgcggtact cgggctgaga atgggccaga aagacgagtt
    31561 tatagcggct gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat
    31621 gtaactcatg aagggcgaag atacggatta gggtgctgtc atcaaatcgt tgtagattca
    31681 ttactgctcc tgaacggtat acaggagtaa gtgtaggcgt tggtaaatga tgtttgtgaa
    31741 gttaatccgc tagcagggca agattacgaa agcgcgctcc ccgcaagcaa ctaatcaagg
    31801 tagttaatca tggctgaaca cgatattacg ccagaagtca ctatttccaa aacacagcgc
    31861 cgctataaag tcggctatgt cagagcccgc caccaggacc gcaaaaccgg tatagcccgg
    31921 tattattcac aacatcccag tctgcacctt aagggcgact ggctgaaaga ggccggattt
    31981 gataccggac ggggtgtcac ggtgaagatt tcggaggggg gcctgaccat catcgctgac
    32041 agtgacgagg tgcaggaact gcgggatggg atgtttagtg tgctgaacga aagttaaata
    32101 ttaaaaaagc tgggaagatt taaaaccttc ctagcttttt attctgtctt attataatgg
    32161 atactcatca tcttccgatg aatttataaa ttgctccaag tttacatgaa gagtacttaa
    32221 cctagttttt aactcattaa gtgaagcgat aatttcatat tttccaacat aatcatatga
    32281 tggatcgtat tcattatcta tgtcagaagc taaaaatgat gatattcgta gtttaaattt
    32341 agcttcatct ttattacatt cattggcata aggtgaatgc cctatcatag catattcatc
    32401 taatctaaaa ggataagatg gttttaattc atcgatcaat gaaatttcaa tccatgttct
    32461 atatccaggc attaatggac taatttcttt gagcaaaacc tttccactta ataatgtatt
    32521 gagattgttc attattatgt ctcccacggg aaaaatatat gtctaacact tccaccatgt
    32581 tgatgaactt gtaaatgtgg taaatcacca tcaggatttc ctggcccatg acctgacact
    32641 ctatatgaag gttttgttac agaaggatct tttatatcat cccaatgata tgaacctgct
    32701 tttgttttat tttcagtaag atattccatt aagttcttag tgctggggcc tgactcacct
    32761 gtcatatttt tataattatg acctaaatat ctatccataa acaattgttc agcgtcactt
    32821 ctagacctaa cagtaacttt ggtttcacta ctatctaata aacgattcgc tttttggtta
    32881 ctaacagcac atggagttaa tccaagtgga tcagcatatt ttagaggatt aggtgcataa
    32941 ctgtagagat ttaaaccgcc tctcaaccct atcgggtcag ttaccatgaa ccgcccgaca
    33001 tccggatcat agtacctgtg cagattgtag tgcagccccg tttccctgtc gaggtactgt
    33061 ccctggtagc gcaggttctg ctccaccccg ccgtggtcct tctcctgtat cgtgttaccc
    33121 cagacctgat accccgtctc ccacacgatt ttcccgtcgc tgtccgtcat ctcttccggc
    33181 gcgccgttca catccgtgtg aaaatacagt atccggttcg ggccctcttt gcccgcctta
    33241 tccacccgcg ccagcggttc ataactcccc tggtccctgt agatgtacag gctgcctgtt
    33301 ccctccggcc tgctctcctg cgccagccgc attccgtccc ataaaaattt tgtccggttg
    33361 tacggcttac cgtcagggct gatgtgctgt ttttccgtcc gcctccccag tgcatcgtac
    33421 acataccggt atgtcccccg cgctgtcgtc acttccacca tccggtgttc cgcatcataa
    33481 cggtaactct gcgttccgct ccggccctcc ttttccaccg cacggccaaa ttcgtcataa
    33541 cggtagtgca caccacggta agacatcagc cggttgaacg gcaccacgct gttttctgcc
    33601 gtggcccttt cccccgcgcg cctccccagc aggtttcccg ccgcgtccac ctccgtttcc
    33661 ggctggtgcc acaccgggtg ccactgcgcc tgctccaccc gccccagcgg gtcgtgcgtc
    33721 tccgtgatat gcccctgcag gtcatacaca tagcgccatt tgccgccctg agggtcggtc
    33781 atccccagca acagtcgttc ttcgtcgctc cagcggaatg tcgtcacctg tccggcttcg
    33841 tcctggtacg ccgtcacctg attctgcgca tcccactggt gataacgtgt gcgccccagt
    33901 ccgtccgtca cgcacagcct gcgctgcgcc aggtcccacg ccagccggta ctgttcaccg
    33961 ctgtccgtgt ggtgccccgt cacccgccat gccgcgtcat ccggaccttc ataccagccc
    34021 cactggtaat aatacccttc gccgcccggc tggcggtgca gcgtcatgca gtggttttcg
    34081 tcgtaagcaa actcacgcag caggtgcccc gtgctgtcgt acacgccgtt caggtggccg
    34141 gcctcgtcat agctgtaacg acgcagcagt aaaggtgcag tctcagggaa atgctgataa
    34201 atctgtgtca cccgccgggg atgcgccgcc agttcgtaat acagacggat gcacggacgc
    34261 tgctgttcgc cgctgatttc cacaatgcgg ccccggtcgt cgtagtacag attcagacag
    34321 ttgctgttac ggtcgcccag cattttcagc cgctggcgct gcgggtggtg cgggtcagcc
    34381 tcaaacagaa aacactgccc gtcatcgctg ctgataagcc agtgcccctg ctcaccacgc
    34441 cttaccgcca gcccttcacc ggggctgtaa aacccgcccc ccggcagtac ggcttccata
    34501 tccaagcgtc gccccatgcc cgacacaaag gtcatgcagt tttcgtctgc gctacccggt
    34561 gtgcacacga ggctgacttc atacattaca ctccagccct gaccaaacat tccttccgta
    34621 cgttcgtcac gactgctgta cacgcgctgc cagtccagcg gaaagtggcc cggcagactg
    34681 aaatcgaagt catcgtcatc ggccaggtat ttggccccgg tggcggcatt gaccggatgc
    34741 ggaagggcac tggtggcctt ctgcgtcagc atgctcatcc cgacactggc agcaaaacag
    34801 gcgattttag aaagcatttt gcccgggcga agaaacatca gcgccacggt tgccaccagg
    34861 gttatctgcg acctgccgct tctgatatcc ctgaccacca gtaacggacc gccaatgcgc
    34921 acatccggtg acacaaacgc cccgttcccc ggctcattca ccaccctggc ctcgcaggta
    34981 cagcgtgccc cgctgcgtgt cgccggctgg ctgttgatac gcacatcccg cgcgccttcc
    35041 gcgatatatt ccgctgcggg cggctcgtca tcgcctttga acagattact gatgctgcca
    35101 ataaccgcat tcactccctg aaaggcggtt tccagcatac cgcccggatt cagcaggtta
    35161 agggccgttc ccaggggggc atccagaaag gcggcttttt tttgtgcgag aaactgcgtc
    35221 agtgaggaag gatgtttatg gcagtcgatt ttatcgtcag gggtcggggt ggatcccggg
    35281 gccgcactgg ccacagtggg ctgccatagt tccgacacaa aatgtgtcac gccatccacc
    35341 atttcagaac gcccttcttc cgtactgagc tggtcgtgca gagcccggga catcagcaca
    35401 ggccctgtca ccgggttaac cgccagggct atggcccccg ccatttccag atattccccg
    35461 gccacatccc ggaacgttct ggcatcctcc tgacgctgcg cttcttcctg ttctgccttc
    35521 gcctgcgctt cctgcgcctc tatgtcctgc cgcgatgccg cagtcgccgc tgcccgcgcg
    35581 gcaggtttgc tgttgatcca tgtgtcacca gagccggtga ggatgtaacc ttcaatctga
    35641 ggcgggaaaa gcatatttgc gagggctgaa caggcttcgc tgaccttttc tgtcacgccg
    35701 gtgtacacca tcgccacacc cgccacaaaa ccggtcagca cgacggccgt gcaccccatc
    35761 gtgatcccca ctgacgtcgc cagtaacgag gttgtgccga caatgagggc atcaataacg
    35821 acatttgcgg ccacctccag caccgccccc agcacatctg ccataaacga cgtatgcagc
    35881 agggcatcac cctgtcttgc cgcccagaat gcctcttcca tgtcccgttc cctgtatgat
    35941 taattcgtcc tgacttcctg ccgcatcctg aagcgcagat tctttttaat ttcagcccag
    36001 cgcgtcatat ctgccggggt cagcggtgtg gttctggcgt aggtgaaggc catcatgacc
    36061 cgctcacccg gcaatacaaa tgccacctga cactggtaca cctgctcgtt accccggaga
    36121 aactggctct gtatttccaa accgcctcgt tgagataata ttccgtcata atgtaaatag
    36181 tccgttttaa taaaggaatt atttattgac aggttctgtt tttttaatca tgaatgtaat
    36241 acccatcacc gctttacagc acactttatc tttccagtca taatcactaa taatattaag
    36301 caattagcaa tatttacaac gaccagagcg gatacatagt tatgtattta acaatcccaa
    36361 tatattcact atgcaaataa aaccactagt aacagacacg ctaccgcctc catataaaaa
    36421 tggacacctt ataaaattgt atcttgcata gaaaaagctg aaatatcgga tgcgttttaa
    36481 ttatcaacta taaatctata agctttttat taacaac
//
LOCUS       DAZOYF010000021        36250 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZOYF010000021 DAZOYF010000000
VERSION     DAZOYF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.021"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1101)
                     /locus_tag="ACHUOF_001024"
     CDS             complement(<1..1101)
                     /locus_tag="ACHUOF_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR0610301.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHUOF_001025"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHUOF_001025"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR0610302.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHUOF_001026"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHUOF_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR0610303.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHUOF_001027"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHUOF_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR0610304.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHUOF_001028"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHUOF_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR0610305.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHUOF_001029"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHUOF_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR0610306.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="ACHUOF_001030"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="ACHUOF_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR0610307.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHUOF_001031"
     CDS             complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHUOF_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFR0610308.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHUOF_001032"
     CDS             complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHUOF_001032"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR0610309.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHUOF_001033"
     CDS             complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHUOF_001033"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR0610310.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHUOF_001034"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHUOF_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR0610311.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHUOF_001035"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHUOF_001035"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR0610312.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="ACHUOF_001036"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="ACHUOF_001036"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR0610313.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="ACHUOF_001037"
     CDS             11851..12015
                     /locus_tag="ACHUOF_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610314.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="ACHUOF_001038"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="ACHUOF_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR0610315.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="ACHUOF_001039"
     CDS             13524..13769
                     /locus_tag="ACHUOF_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR0610316.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="ACHUOF_001040"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="ACHUOF_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR0610317.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="ACHUOF_001041"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="ACHUOF_001041"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR0610318.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="ACHUOF_001042"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="ACHUOF_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR0610319.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="ACHUOF_001043"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="ACHUOF_001043"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR0610320.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="ACHUOF_001044"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="ACHUOF_001044"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR0610321.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="ACHUOF_001045"
     CDS             19432..19785
                     /locus_tag="ACHUOF_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR0610322.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="ACHUOF_001046"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="ACHUOF_001046"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR0610323.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21085..22212
                     /locus_tag="ACHUOF_001047"
     CDS             21085..22212
                     /locus_tag="ACHUOF_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFR0610324.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22280..24214
                     /gene="rnb"
                     /locus_tag="ACHUOF_001048"
     CDS             22280..24214
                     /gene="rnb"
                     /locus_tag="ACHUOF_001048"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR0610325.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24292..24395)
                     /locus_tag="ACHUOF_001049"
                     /pseudo
     CDS             complement(24292..24395)
                     /locus_tag="ACHUOF_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24449..26423
                     /gene="pdeR"
                     /locus_tag="ACHUOF_001050"
                     /pseudo
     CDS             24449..26423
                     /gene="pdeR"
                     /locus_tag="ACHUOF_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACHUOF_001051"
     CDS             complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACHUOF_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR0610326.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            26571..26744
                     /gene="yciZ"
                     /locus_tag="ACHUOF_001052"
     CDS             26571..26744
                     /gene="yciZ"
                     /locus_tag="ACHUOF_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR0610327.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26834..27583
                     /locus_tag="ACHUOF_001053"
     CDS             26834..27583
                     /locus_tag="ACHUOF_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR0610328.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27852..28070
                     /gene="osmB"
                     /locus_tag="ACHUOF_001054"
     CDS             27852..28070
                     /gene="osmB"
                     /locus_tag="ACHUOF_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR0610329.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACHUOF_001055"
     CDS             complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACHUOF_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR0610330.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACHUOF_001056"
     CDS             complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACHUOF_001056"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR0610331.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACHUOF_001057"
     CDS             complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACHUOF_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR0610332.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACHUOF_001058"
     CDS             complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACHUOF_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR0610333.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACHUOF_001059"
     CDS             complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACHUOF_001059"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR0610334.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32019..32609
                     /gene="ribA"
                     /locus_tag="ACHUOF_001060"
     CDS             32019..32609
                     /gene="ribA"
                     /locus_tag="ACHUOF_001060"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR0610335.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="ACHUOF_001061"
     CDS             complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="ACHUOF_001061"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR0610336.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMFIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35512..35607)
                     /gene="ymiC"
                     /locus_tag="ACHUOF_001062"
     CDS             complement(35512..35607)
                     /gene="ymiC"
                     /locus_tag="ACHUOF_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR0610337.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35721..35888)
                     /gene="yciX"
                     /locus_tag="ACHUOF_001063"
     CDS             complement(35721..35888)
                     /gene="yciX"
                     /locus_tag="ACHUOF_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR0610338.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35891..36031)
                     /locus_tag="ACHUOF_001064"
     CDS             complement(35891..36031)
                     /locus_tag="ACHUOF_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR0610339.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT         8601 a         9423 c         9113 g         9113 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaataat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta
     1081 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaaaaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
     4861 ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat
     4921 tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca
     4981 ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc
     5041 cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg
     5101 cggcaaccag ctcttttggc gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa
     5161 taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga
     5221 gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac
     5281 cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca
     5341 tatatgtacg accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg
     5401 cgcgagcaat tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt
     5461 tagcctgccc gctcactggg gcaagggtgt taattttctc cgccagcgtg acgtagcttt
     5521 cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg
     5581 cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga
     5641 tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt
     5701 tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt
     5761 tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga
     5821 tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg
     5881 ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgctcggc gtcaccgcgc
     5941 agcagttcag caatcaaacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa
     6001 ccctgcatgt aatagatgtt ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc
     6061 agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcaactg agggaaggtt
     6121 tttagtagtt tcggtaccac aagacgctca acgtcatccg ggttgcgtgc accatacact
     6181 acacccccgc cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag
     6241 ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc
     6301 cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgctag ttccggctct
     6361 actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacca
     6421 ttggcagtcc gaaccacggc tggcgtggtg tgctggatct gcgtcacggc ggaaagctca
     6481 tacacgcgcc cgccgttcag gcggatggca tcggcttcac caatggcgag gtttagcggg
     6541 tgaatatgcc caccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg
     6601 cggcgaatag cgttgacgtc cagcagctcc agctgtttat tgccgtagcg tacccagttc
     6661 tctttctgct cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa cagaccgccg
     6721 gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc
     6781 tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg
     6841 tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag
     6901 ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg
     6961 gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc
     7021 agtgtgtcga atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg
     7081 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc
     7141 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
     7201 atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg
     7261 gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt
     7321 ggcaagctgc agcgcctgat cttctgatgt gaaacgggtg accaccagca ccggaccgaa
     7381 aatctcttcg cggcttaagg acgcattcgg gtccacatcc acgaagatgg tcgggccgat
     7441 ggccgctgcc agcccggcgt tgcgaccatc cagcagcagt tgccccttgc cttcgccttc
     7501 acgaataaag gtgtgtaccg agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt
     7561 tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
     7621 gaattcatcg gcgatgctct cttccagtaa caaacgcgtt ccggcgatgc gcacctggcc
     7681 ctggttgtag aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc
     7741 ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacacgtt tcatgttgct
     7801 atcacccgcg tctttcagca gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc
     7861 atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
     7921 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact
     7981 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
     8041 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
     8101 gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata
     8161 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
     8221 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt
     9001 gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaaatg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaactca atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctagtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcaatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagagctc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg taactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtacag ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    21001 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    21061 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    21121 tatcatgaaa cgcaatccag tactacggag tatgacgttc tgcctctggt cccggaagcc
    21181 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagagac gctggccccc
    21241 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt
    21301 accgtgaccc gttcacaaac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt
    21361 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    21421 cccggacctg attcctccag ggaaagtaat catcggttgg cacaaatcac gcaatatgcc
    21481 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    21541 gtcggtctta ctgtctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt
    21601 caggcgcgga caattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc
    21661 gggctggcga tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    21721 cgaagcagct atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa
    21781 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag
    21841 ttgttaaaca gcacacgggt caatgcacag ccggataatc agcttgcggc gttgttatgc
    21901 gcgcgtataa atggcagtcc tgcttgtttt tccacctgta tggattcatc aaatgaatat
    21961 aaaaaaatca gcacccttct gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt
    22021 cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    22081 tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt
    22141 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    22201 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    22261 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    22321 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    22381 cttcctggaa gtcgacgcac aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    22441 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    22501 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    22561 tgaccgtctg accatcgttc ctgatcatcc acttttaaaa gacgccattc cttgccgcgc
    22621 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    22681 tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg
    22741 tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc
    22801 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgacctc
    22861 gctggatttt gtcaccatcg acagtgccag cactgaagat atggatgacg cccttttcgc
    22921 taaggcgctg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22981 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    23041 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    23101 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat
    23161 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga
    23221 ccaggtttct gactggctgg aaaataccgg tgactggaag cctgaaagtg aagcaattgc
    23281 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    23341 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    23401 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    23461 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    23521 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    23581 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    23641 tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    23701 tgctgaaatt agctctgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    23761 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    23821 tatcaaaggc gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg
    23881 tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct
    23941 gaaagataaa gccgggaccg gcacccgttt cgcggcggaa attgtcgata tcagccgtgg
    24001 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    24061 acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    24121 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    24181 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg
    24241 gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac
    24301 tttcgtttga aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaca
    24361 atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga
    24421 ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta
    24481 caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgctttgcg
    24541 cttttctacc accaaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc
    24601 tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga
    24661 tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg
    24721 caacgcgtct gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc
    24781 ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg
    24841 gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt
    24901 cattgggtaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    24961 caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac
    25021 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    25081 aaacgagatt tttttaatct gttccggtac cgacattacc gaagagcgcc gcactcagga
    25141 gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat
    25201 gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg gggttgtgta
    25261 tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca
    25321 gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc
    25381 gcgtccaggt ggcgatgagt ttctggtact ggcatccaat acctcacaaa gcgcgctgga
    25441 agcaatggca tcacgaattt tgacccgctt acgcctcccc tttcgtattg gtttaattga
    25501 agtttatacc agctgttcag taggtatttc gctctctccc gaacatggtt cggacagcgc
    25561 gactattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca
    25621 attttgcgtt tttactccag aaatgaatca gcgggtattt gaatatctct ggctggatac
    25681 caacttgcgt aaagcactgg aaaacgatca gttggttatc gaaaatcacc tagcgtgtcg
    25741 aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
    25801 cgttggactt catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg
    25861 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag
    25921 tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
    25981 aggttctcca ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt
    26041 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
    26101 cgcaagtgca tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
    26161 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
    26221 tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
    26281 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg
    26341 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
    26401 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca
    26461 ttatatatcg tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg
    26521 gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat
    26581 ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt gtggcaggcg
    26641 attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg cgccaggttg
    26701 ctgaatctac gccagacatc ccgaagacac cgtgggagat ttaacaatcg tgccttcagg
    26761 ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt ataacgttat
    26821 aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca ttgaccaggg
    26881 tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta ccattcgcca
    26941 ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct ttgcagtttc
    27001 gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga agcgtgaact
    27061 cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg aaaatggcag
    27121 cagcaatgcc ctgctcgccc ggactctggg ggagcagaag aaaaatgtca ctatcatcac
    27181 ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtca ttttgctcgg
    27241 tggcgtgtac cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc agtgcatcca
    27301 gcaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg aaactggatt
    27361 caccggtcgc gatatgatgc gcaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga
    27421 agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact ccatcggtcc
    27481 cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg atctgatgca
    27541 tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc ccttctctac
    27601 ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg ataattaaga
    27661 ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat ataacaggaa
    27721 gtaattatca cctgcgtgat ataaccctgc gcgcgagcag atttcacgga ataatttcac
    27781 caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc ataaattcag
    27841 gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg gcaattactt
    27901 tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac accgcaatcg
    27961 gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg ttgggtacat
    28021 taggtggtgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa
    28081 atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg aaataactga
    28141 ataatacaca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt ttatgttaac
    28201 cgcctgcgag ttttaccttc atccctttcg cttcaagtag tgattttaat aaatcacgct
    28261 tatcgccctg gatttcaata actccatctt taactgctcc gccgcagccg cattttttct
    28321 tcagttccgc tgcaagtttt gtcagttcgg catcatcgag atcgataccg gtaatcaggc
    28381 aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccgct ccatcgcctt
    28441 tagggcgtac tggggccgct ttgggctcat caatacgccc ggtttccgtt gagtagacca
    28501 gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggcgttga tcgctttcag
    28561 tgtctgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat aatcaacacc
    28621 agccgccaac gcctgttctg gcgtcataat gcggcgctgg tcaccagctt cactcccctg
    28681 cggacgaatg cccggtgtaa ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac
    28741 agcttcctga gcagaacaca ccacaccatc aaggtcacat ttttgcgtca gtgctgccag
    28801 acgttctgca taatctgcag gtgacagtgt cacgccaaga tcggccaggt cgctggcttc
    28861 catgctggtc aacactgtca cagcaatcaa aagcggtgca tctttgccaa acggaaccag
    28921 tgcctcacgc gctgcggtca tcatacgcgc cccaccggag gcatgaacat tcaccatcca
    28981 cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttgggga tatcgtggaa
    29041 tttcaggtca agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc
    29101 aaacaatgta aacatctctt taccgacctt cagacgacaa tcgcgtgggt cgatcttgtc
    29161 gacaaaggac agcgcgtcat cacaattatg ataatcaagg gcaacaacca caggagaatt
    29221 cgtaacggcg cgggaagaag atgaagcagt taacgtcatg accagacctt cttgatgatg
    29281 ggcaccggga gtggcgcgaa acaggtaaac ggcgagcatt ctacccgtga aaataaaaaa
    29341 ttaacagttg cgatttttcc cctagcaggc tttttctgct ccgtcgttgc cggagcacaa
    29401 tgggacatca ataactagta tgttgtaact aaagtcggat ttttttaaaa attacaggcc
    29461 atcaagaccg cgaatcggtt taatagttga ccaggcacga caagacggac aatgccagta
    29521 gagggtgtat gcggtaaaac cacatttctg gcagctataa cgaggcttac tacgcacctt
    29581 ctcgccaacc atgtcacgca gcaccatcag actctctttg gcgcgccctt cttccgcttc
    29641 atttaagtgg taatccatta gcttatggaa tacacgcatg gtcggatgac gctgaagctg
    29701 gcgtgtaata tagacctgtg cagcctcact accgtcgcgc gcttcgatga tatcagcaag
    29761 catcaattca gcatcggcac cggtgttctc ttccaccgcg cgttgcagga attctgccca
    29821 ttcggcagtt ttacccaact gctgatagca ggtttgcaac atttccagcg tttcgctgac
    29881 cagttctctg tcctgggata tgacgcgttg caaactttcg acggctttgg cgtattctcc
    29941 tttcgccata aacacgcgtc ccatcatgat ggatacgcgg gcgctgtttt tatctgccgc
    30001 cgcccctttt tttagcaagg tcatggcacg atcgagatcg tcgctggcca tatgctgcag
    30061 ggctaactca cagtagaaat gggcaatttc gacgcgctgt ttatctttac ccagcttcac
    30121 caggcgttcg gcaacatcaa ttgctttctg ccactcactg gtagcctggt agatttgtag
    30181 caactgttgc agcgcgccaa tgcggaagtc agtttcatcg gtcagctgat tgaacatgtc
    30241 ttccgcgcgg tcgtataacc cggcggccat gtaatcacgc cccagttgtt gaatcgccaa
    30301 cagacgctgt tcataggtca gcgaggcgct ttccattagg gtctgatgga tgcgaatagc
    30361 gcgatcaact tcgccacgcg aacggaacag gtttccgagc gtaaggtggg cttcaacggt
    30421 gcctgtatcc tctttaagca tatcgagaaa caggtctacc gctttatcct gttgattact
    30481 aagcaggaag ttaacccccg ctacgtaatc acgcgacaag cggttggctt catcttgctt
    30541 gttttgttgc gcacttctgc ggcccatata ccagccatag gcagcggcta caggcaaaag
    30601 cagaaacaac aactccagca tagaaagtta ttccttcgcc gctgacgagt gcggcactac
    30661 agccacgtca gtcgcgggtg aaagctggtt ttccagtcgc tttattttac gttcagcgcg
    30721 cgccagggaa acacgaactc gcagccagaa caggccacaa atcaaccaac cgatagcaaa
    30781 ccccgcagca aacaataccg ccagcaatgt ggaaatacgg tactcccctt gcgctaacag
    30841 ataattaaac gtcacctgtt gatcgttctg cgcacccaac gtgaccgaaa tcacgaagat
    30901 cgctaacacc agtaagaaaa tgagtaaata tttcacatta cttcccgtta tgtggttaga
    30961 gcgaataaat tatggtcaac ttaccgcaat agccctttaa agtaccattt tcgcgccgtc
    31021 tgcgaaatgg catccacgcg cattgttgat gatttaaggc ctaaattcgg aaaatcaacc
    31081 agcgttaact ttcttgttct cgttgtgcaa tttcgcgatt ttcttccgca ggtggtgtta
    31141 atggcccaca aattcgttgc gcaagccacg ttgccaccgc caccagcgcc cacgaaatca
    31201 acgtagctac caccagatcg cgtggccaat gcatcccgag cagcaggcgg cttcccatga
    31261 ctcccgttgc ccagaccaac aagatagcaa tggttaacgt tcgccgacgc ggccacagca
    31321 aaccaacggc cagcagtgcc caactggcag caaacatcgt gtgaccggaa ggaaaggcaa
    31381 accccgtctc tttctgccag tgtgaacgca aatattgtgg aatatttttc tcttcagcca
    31441 actgttcttt tactagattt ccgcgttctg ctcgctttaa agtgtagaac tcatcaaccg
    31501 gaatatgatg tgttttttcc aaccagataa caaaaggtcg tggttcctgt actttgtctt
    31561 tgatccagga tttaacgcct tgtccaacaa ggattgccgc cgccagaatg gcaaataata
    31621 caaaggcagc cttaatgcga aaacgcagac accagagaaa ccagccgaac aaaatcaaat
    31681 gtgtaatgac gccccagggc tgggtgacag tttcagtaac ccaaaaagcc gcttttagta
    31741 gccaactttg ttctccaggt tgccaacgcc agccagaaat ccatacggct actggcatga
    31801 caagcaatag tgcagctccc actgcggtac gtcgggcaat cgaacgcatg gcctctcctt
    31861 tttgataagt cccacaatca taactgaaaa tgccagttcc aagaaaaatt gacagatttg
    31921 tgccattccg tgaacgatcg acgcgtcgtg attaggtgaa ccccttctcg ttatggcaaa
    31981 ataagccaat acagaaccag cattatctgg agaatttcat gcagcttaaa cgtgtggcag
    32041 aagccaaact gccaacccca tggggcgatt tcctgatggt gggatttgaa gaactggcaa
    32101 ccggacacga tcatgttgcg ctagtctatg gcgatatttc cgggcatacc ccggtacttg
    32161 cgcgcgtcca ttccgaatgt ctgaccggtg acgccctgtt cagcttgcgc tgcgattgtg
    32221 gcttccagct cgaagcggca ttgacgcaaa ttgccgagga aggccgtggt attttgctgt
    32281 atcaccgtca ggaaggtcgt aacattggtc tgctgaataa aatccgcgct tacgcactgc
    32341 aggatcaagg ttacgatacc gtagaggcta accaccagtt aggcttcgcc gctgatgagc
    32401 gcgacttcac tctttgcgct gatatgttca aactccttgg cgtcaatgaa gtccgcttgt
    32461 taaccaataa cccgaaaaaa gtcgaaattc tgaccgaagc agggattaat attgttgaac
    32521 gcgtaccatt gattgtaggt cgtaacccca ataacgaaca ttatctcgat accaaagccg
    32581 agaaaatggg ccatttgctg aacaaataac cctcttgcat cgtgtaattc atttgcttgc
    32641 cggaagcaaa ataaccggca agcaaatact tgttacttca acatattacg aatgacataa
    32701 tgcaaaatgc cgtcgttctg gtagtaggtc aactccgtcg cggtgtcgat acgacaacgg
    32761 cagggtacga cttcctggct accatccgcg cgcgtaagcg tcaccggaac cgtcgcgcca
    32821 ggttgtagat tttgcagatc gccaatatca atcttctctt ccccggttag ccctaacgtt
    32881 ttacgcgtca cgccttgcgg aaattccagc ggcaggatgc ccatgccaat taaattcgaa
    32941 cggtgaattc gttcaaacga ttcggcaatc accacacgaa ttccaagcag acgaggtcct
    33001 tttgccgccc agtcacggct tgagcctgat ccatactctt tcccggcaat caccgccagc
    33061 ggcgtttgct cctgcttata gcgcatcgca gcatcataaa tagagactac gtcgctgtca
    33121 ggtaaatgcc gcgtcatccc cccttcaacg ccaggcacca tttcattacg gatgcgaata
    33181 ttggcgaagg tgccgcgcat catcacttca tggttaccac gccgcgaacc gtaggagtta
    33241 aagtcttttc gctcaacacc ccgaccttgt agatatcgac ccgctgggct gtcgggctta
    33301 atactgcccg ccggagagat atggtcagtg gtgactgaat cccccagcat tgcgaggatc
    33361 cgcgcaccgt gaatatcttc cactggtgct ggtgttgcct gcatttcatc aaagaaaggc
    33421 gataagcgaa tataggttga gtcctcctgc caaccgtagg tatcggatcg tgtgacgtta
    33481 atttccttcc actctgctgt gccttcaaaa acttctgcgt actctttgcg gaacatttct
    33541 gtggagactt gttctaccgc acgggcaatt tcttgtgccg atggccagat atctttcaga
    33601 taaaccggct cgcctttgcg atcatggccg ataggctcag aagccaggtt gatattcata
    33661 tttcccgcca gcgcataggc aaccaccagc ggtggcgagg ccagccagtt agttttaacc
    33721 agcggatgga tacggccttc aaagttacgg ttgccggaca gcaccgcacc gacggttaaa
    33781 tcgccttttt tgattgccgt ttcgatagga tcgggcagcg gcccagagtt accaatacag
    33841 gtggtacaac cgtatcccac aaggttaaac cccagttcgt cgagatacgg tgtcagtttc
    33901 gcttttgcca gataatcaga aacgactttc gaacccggtg ccaacgacgc tttgacccac
    33961 ggttgccgct tgaggccaag agttacggct tttttcgcca gcaagcctgc ggccatcagc
    34021 acacttgggt tagaggtgtt ggtgcacgag gttatcgcag caatgaccac agcgccatca
    34081 ggtaactgat actgatgtcc gttcataaca taatcgactg gcaggcgatc tttatgcgtg
    34141 gcattcactt tcagttcgtt actggcggca aatgcttttg gtacatcggg cagtgcaacg
    34201 cgatcctgtg ggcgttttgg ccctgccagg ctcgcttcaa cgtcattcat atccagttct
    34261 aacatactgg taaaaatcgg ttcatcgccc gggttacgcc acatgccctg cgctttggca
    34321 tatttttcga ccaactcgac ctgatcttcg ctgcgcccgc ttaaacgcat gtaatcgagg
    34381 gttactgcat cgattgggaa gaagccacag gtggcaccat attctggcga catattggca
    34441 atggtggcgc gatccgccaa cggtagtgaa tccagaccat caccataaaa ttcgacgaat
    34501 ttccccacca cgccatgttt gcgcagcatt tgggtaacag tgagaaccag gtctgtggcg
    34561 gtaatacctt cacgtaattt tccggtaagt ttaaagccca ctacgtccgg gataaacatg
    34621 gaaaccggct ggcctaacat ggcggcttct gcttcgatcc cacccacgcc ccaccccagc
    34681 acgccaaggc cgttgatcat agtggtgtgc gagtcagtac caacgagtgt atccggataa
    34741 gcaatccatt caccgtcctg caattcgctc cacactgctt tgccgagata ttcgaggtta
    34801 acctgatggc aaatgcctgt gcctggcggc acgacgctaa accgactgaa cgcttgcttt
    34861 ccccatttca ggaacacata acgttcgtgg ttgcgctcca tttccaggcg tacgttttct
    34921 tcaaatgcct catcatcacc aaaacgatcg acggtcaccg agtggtcaat gaccaggtcg
    34981 accggtgaga gcgggttaac ctttgcagta tcgccaccga ggcgtttaac cgcttcgcgc
    35041 attgccgcca gatcaacaac ggcaggtacg ccggtaaagt cctgcatcag cacccttgcc
    35101 gggcggtagg caatttcacg gtcagcatgg gcatttttca gccatcccgc cagtgcgtgg
    35161 atatcctctt cggtaactga gttaccatcc tgccagcgca gcaggttttc gagcaaaact
    35221 ttgagtgact tgggtagacg ggtgatatcg cccagtgatt tagcagcaag cggcaggctg
    35281 tagtagtggt aagttttatc tttggcctgc aacgtgtcct tactggcttc tcgtagggtt
    35341 gacgacatag ctcctcctta atgacagggt tgcgaagccc tgattctctt cagggtgaat
    35401 attaaagata acacaaacat cgcgtaacga tttgataaca acccaaattg ataaaagagg
    35461 agaaaccttg tggcaaagca aatgacaacc ccgccgcagc ggggtcaagg atcagtgagt
    35521 aatgatccag aggagttcgg cccagaagag catcgacaga gaaaacgcgc ccatccagga
    35581 ccaatatttc atatttgtgt tgatcatagg ctacaccctt aacggttatt attttgtgtt
    35641 gagataactc aagctgttat tgctgcggtg tattaaaagc cctttaattc attaaaaatg
    35701 ggtaataaga atgatgataa ttattgtttc ggctgatcat cttcagcaaa cagttctata
    35761 aaggcttgct gctgcgatgt tagttgccag tcgtctggaa taacgacgga acattcccgt
    35821 ttcgtttctg gttcattgtc actatgctgg cataatggtg acgactccag ttgctcctga
    35881 ccgaccatag ttatccccac actttccaaa tcagcagtgc gacaaccacc cagaaaagtg
    35941 cagaaccaag aaaaaccgcc agccaggctt tacgttttaa ttcaggatca cggcggggtt
    36001 cctgatttcc agaaggcatt gctaacctca tataatcgcc atcacttatc agcaagacgt
    36061 tgaatgattg gtgtaattaa tcatacgatg agacaaatcc taaagaaact ttagtcaaaa
    36121 atccagcgaa tttacgaatc gtctccagtg aataattcaa tcttttgacc tgaaatagat
    36181 atttgaaaac tggaatttgc gaggcgggta attagacact actttacagg taatttaccc
    36241 tataaatatg
//
LOCUS       DAZOYF010000022        34999 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZOYF010000022 DAZOYF010000000
VERSION     DAZOYF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.022"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHUOF_001065"
     CDS             complement(<1..815)
                     /locus_tag="ACHUOF_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0610340.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHUOF_001066"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHUOF_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHUOF_001067"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHUOF_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1398..1874)
                     /locus_tag="ACHUOF_001068"
     CDS             complement(1398..1874)
                     /locus_tag="ACHUOF_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR0610341.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="ACHUOF_001069"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="ACHUOF_001069"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR0610342.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="ACHUOF_001070"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="ACHUOF_001070"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR0610343.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="ACHUOF_001071"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="ACHUOF_001071"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR0610344.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="ACHUOF_001072"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="ACHUOF_001072"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR0610345.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="ACHUOF_001073"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="ACHUOF_001073"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0610346.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7491..9512
                     /gene="uvrB"
                     /locus_tag="ACHUOF_001074"
     CDS             7491..9512
                     /gene="uvrB"
                     /locus_tag="ACHUOF_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR0610347.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="ACHUOF_001075"
     CDS             complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="ACHUOF_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR0610348.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10877..11021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11009..11998
                     /gene="moaA"
                     /locus_tag="ACHUOF_001076"
     CDS             11009..11998
                     /gene="moaA"
                     /locus_tag="ACHUOF_001076"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR0610349.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12020..12532
                     /gene="moaB"
                     /locus_tag="ACHUOF_001077"
     CDS             12020..12532
                     /gene="moaB"
                     /locus_tag="ACHUOF_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR0610350.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12535..13020
                     /gene="moaC"
                     /locus_tag="ACHUOF_001078"
     CDS             12535..13020
                     /gene="moaC"
                     /locus_tag="ACHUOF_001078"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR0610351.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13013..13258
                     /gene="moaD"
                     /locus_tag="ACHUOF_001079"
     CDS             13013..13258
                     /gene="moaD"
                     /locus_tag="ACHUOF_001079"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR0610352.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13260..13712
                     /gene="moaE"
                     /locus_tag="ACHUOF_001080"
     CDS             13260..13712
                     /gene="moaE"
                     /locus_tag="ACHUOF_001080"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR0610353.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13849..14553
                     /locus_tag="ACHUOF_001081"
     CDS             13849..14553
                     /locus_tag="ACHUOF_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0610354.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14758..15471
                     /locus_tag="ACHUOF_001082"
     CDS             14758..15471
                     /locus_tag="ACHUOF_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0610355.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15507..16463)
                     /locus_tag="ACHUOF_001083"
     CDS             complement(15507..16463)
                     /locus_tag="ACHUOF_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFR0610356.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="ACHUOF_001084"
     CDS             complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="ACHUOF_001084"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR0610357.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17701..18462)
                     /locus_tag="ACHUOF_001085"
     CDS             complement(17701..18462)
                     /locus_tag="ACHUOF_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0610358.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18595..19005
                     /locus_tag="ACHUOF_001086"
     CDS             18595..19005
                     /locus_tag="ACHUOF_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR0610359.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18967..20073)
                     /locus_tag="ACHUOF_001087"
     CDS             complement(18967..20073)
                     /locus_tag="ACHUOF_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0610360.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20084..21217)
                     /locus_tag="ACHUOF_001088"
     CDS             complement(20084..21217)
                     /locus_tag="ACHUOF_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0610361.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21210..22946)
                     /locus_tag="ACHUOF_001089"
     CDS             complement(21210..22946)
                     /locus_tag="ACHUOF_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0610362.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="ACHUOF_001090"
     CDS             complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="ACHUOF_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR0610363.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="ACHUOF_001091"
     CDS             complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="ACHUOF_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR0610364.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24837..26057
                     /gene="rhlE"
                     /locus_tag="ACHUOF_001092"
     CDS             24837..26057
                     /gene="rhlE"
                     /locus_tag="ACHUOF_001092"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR0610365.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNKRKTA"
     gene            complement(26051..26149)
                     /locus_tag="ACHUOF_001093"
                     /pseudo
     CDS             complement(26051..26149)
                     /locus_tag="ACHUOF_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="ACHUOF_001094"
     CDS             complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="ACHUOF_001094"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR0610366.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26833..28983
                     /gene="dinG"
                     /locus_tag="ACHUOF_001095"
     CDS             26833..28983
                     /gene="dinG"
                     /locus_tag="ACHUOF_001095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR0610367.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29011..29973
                     /gene="ybiB"
                     /locus_tag="ACHUOF_001096"
     CDS             29011..29973
                     /gene="ybiB"
                     /locus_tag="ACHUOF_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR0610368.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30114..31199
                     /gene="hcxB"
                     /locus_tag="ACHUOF_001097"
     CDS             30114..31199
                     /gene="hcxB"
                     /locus_tag="ACHUOF_001097"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR0610369.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            31266..31325
                     /gene="ybiE"
                     /locus_tag="ACHUOF_001098"
     CDS             31266..31325
                     /gene="ybiE"
                     /locus_tag="ACHUOF_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR0610370.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31428..31688)
                     /gene="ybiJ"
                     /locus_tag="ACHUOF_001099"
     CDS             complement(31428..31688)
                     /gene="ybiJ"
                     /locus_tag="ACHUOF_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR0610371.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="ACHUOF_001100"
     CDS             complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="ACHUOF_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR0610372.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="ACHUOF_001101"
     CDS             complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="ACHUOF_001101"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR0610373.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33012..34999)
                     /locus_tag="ACHUOF_001102"
                     /pseudo
     CDS             complement(33012..34999)
                     /locus_tag="ACHUOF_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8295 a         8971 c         9457 g         8276 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga atccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga
     6961 ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat
     7021 gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact
     7081 attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg
     7141 cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact
     7201 ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca
     7261 ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt
     7321 tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag
     7381 aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac
     7441 gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac
     7501 cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc
     7561 tcgaagaggg gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctccg
     7621 ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg
     7681 cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa
     7741 acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga
     7801 gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt
     7861 tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg
     7921 cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg
     7981 gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta
     8041 atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc
     8101 cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat
     8161 tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct
     8221 acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca
     8281 aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc
     8341 agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt
     8401 cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga
     8461 cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca
     8521 ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt
     8581 acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag
     8641 cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat
     8701 ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg
     8761 aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg
     8821 caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttaccg
     8881 aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg
     8941 agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca
     9001 acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg
     9061 acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac
     9121 gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag
     9181 cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca
     9241 ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg
     9301 ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc
     9361 cgatggatat gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga
     9421 tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc
     9481 agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa
     9541 cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa
     9601 tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca
     9661 ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta
     9721 acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg
     9781 cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc
     9841 cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc
     9901 tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa
     9961 ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca
    10021 tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt
    10081 gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag
    10141 ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca
    10201 gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa
    10261 ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt
    10321 ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg
    10381 aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa
    10441 tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga
    10501 tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc
    10561 ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc
    10621 ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg
    10681 ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct
    10741 ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca
    10801 cgatcatgac gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt
    10861 acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata
    10921 cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac
    10981 ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg tatttgcgcg
    11041 taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg
    11101 cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga
    11161 aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg
    11221 aggagagccg tctttacgcc gtgactttac cgatatcatc gccgctgtgc gggaaaacga
    11281 cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa
    11341 ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca
    11401 gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc
    11461 atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca
    11521 tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat
    11581 cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca
    11641 ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga
    11701 cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc
    11761 gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact
    11821 ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac
    11881 ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca
    11941 tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa
    12001 cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc
    12061 tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg
    12121 cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccattgtga aagaaaaccg
    12181 ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt
    12241 gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt
    12301 gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat
    12361 tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt
    12421 cgccatgccg ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca
    12481 gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg
    12541 caactgaccc atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg
    12601 gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg
    12661 atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt
    12721 attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc
    12781 aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta
    12841 tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg
    12901 gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta
    12961 cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa
    13021 agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc
    13081 ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc
    13141 gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga
    13201 ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga
    13261 tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt
    13321 ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc
    13381 ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa
    13441 aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg
    13501 tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca
    13561 gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc
    13621 gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg
    13681 agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct
    13741 gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat
    13801 cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc
    13861 ccacgttctg attcaatcgt acaaccctgg gctggcttgc aaacttatat ggcgcaagtc
    13921 tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat
    13981 tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc
    14041 gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta
    14101 acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc
    14161 attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc
    14221 gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg
    14281 tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa
    14341 gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat
    14401 gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg
    14461 cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg
    14521 atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc
    14581 atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat
    14641 gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg
    14701 tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg
    14761 gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac
    14821 ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct
    14881 aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg
    14941 ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc
    15001 gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt
    15061 cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg
    15121 ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg
    15181 ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt
    15241 tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat
    15301 aagctccttg aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc
    15361 gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc
    15421 gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg
    15481 cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc
    15541 ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa
    15601 tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca
    15661 gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg
    15721 acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga
    15781 ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc
    15841 tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg
    15901 cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg
    15961 ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct
    16021 gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc
    16081 gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc
    16141 ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac
    16201 accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca
    16261 tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg
    16321 cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc
    16381 accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc
    16441 cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat
    16501 cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg
    16561 ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt
    16621 tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg
    16681 cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga
    16741 tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg
    16801 tcgcccagtg atcgtccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa
    16861 aaacctgaac gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa
    16921 tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt
    16981 tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt
    17041 cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt
    17101 cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt
    17161 tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa
    17221 actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct
    17281 ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac
    17341 gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa
    17401 aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga
    17461 caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc
    17521 cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc
    17581 agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg
    17641 gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt
    17701 tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag
    17761 cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag
    17821 tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc
    17881 atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc
    17941 acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc
    18001 aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc
    18061 ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc
    18121 gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc
    18181 gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc
    18241 ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc
    18301 gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc
    18361 gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat
    18421 ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc
    18481 tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt
    18541 ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag
    18601 tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta
    18661 ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg
    18721 ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc
    18781 gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt
    18841 ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg
    18901 gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat
    18961 aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt
    19021 tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata
    19081 aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag
    19141 ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat
    19201 aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag
    19261 accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga
    19321 tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa
    19381 ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg
    19441 cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt
    19501 gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt
    19561 gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg
    19621 ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc
    19681 aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa
    19741 ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt
    19801 gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct
    19861 ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat
    19921 ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat
    19981 tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc
    20041 tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt
    20101 tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt
    20161 ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga
    20221 aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct
    20281 gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag
    20341 cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga
    20401 gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga
    20461 gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag
    20521 ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca
    20581 tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg
    20641 tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa
    20701 accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc
    20761 gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat
    20821 tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct
    20881 gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg
    20941 cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg
    21001 tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct
    21061 tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta
    21121 ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg
    21181 cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg
    21241 tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac
    21301 tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg
    21361 atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct
    21421 ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg
    21481 gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc
    21541 agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg
    21601 ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac
    21661 acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt
    21721 tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc
    21781 agcaccagcg cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc
    21841 gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag
    21901 ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc
    21961 accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag
    22021 gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg
    22081 atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg
    22141 aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag
    22201 gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg
    22261 gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc
    22321 agtaacacgt cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac
    22381 atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca
    22441 acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc
    22501 aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc
    22561 ccaagagacg taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg
    22621 ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca
    22681 aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca
    22741 aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc
    22801 agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg
    22861 gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg
    22921 ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg
    22981 cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag
    23041 gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac
    23101 gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt
    23161 gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg
    23221 acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac
    23281 cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag
    23341 ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc
    23401 ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc
    23461 ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct
    23521 tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc
    23581 ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag
    23641 atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata
    23701 cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac
    23761 cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac
    23821 gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc
    23881 aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac
    23941 aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg
    24001 atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct
    24061 ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg
    24121 gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc
    24181 ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg
    24241 gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg
    24301 caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag
    24361 cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg
    24421 gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc
    24481 tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc
    24541 gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga
    24601 ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg
    24661 gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct
    24721 gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct
    24781 tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gaggttcgga gtagttatgt
    24841 ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc
    24901 gtgaacccac ccctattcag cagcaggcga tccctgctgt gctggaaggc cgcgacctga
    24961 tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac
    25021 acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta
    25081 ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc
    25141 tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt
    25201 tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc
    25261 aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc
    25321 tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc
    25381 agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt
    25441 tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc
    25501 agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta
    25561 aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg
    25621 ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag
    25681 gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga
    25741 ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aactatgaac
    25801 tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta
    25861 ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa
    25921 aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa
    25981 tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca
    26041 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
    26101 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
    26161 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
    26221 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
    26281 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
    26341 caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga
    26401 atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa
    26461 gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga
    26521 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg
    26581 gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt
    26641 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
    26701 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
    26761 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
    26821 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
    26881 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
    26941 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
    27001 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
    27061 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
    27121 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg
    27181 cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat
    27241 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
    27301 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat
    27361 atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac
    27421 cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa
    27481 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
    27541 ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
    27601 gcccgggatg cgctggagat gagcgctgaa atcaccgtgc cctggtatcg actacagctg
    27661 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
    27721 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
    27781 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
    27841 gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc
    27901 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
    27961 cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt
    28021 gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
    28081 accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg
    28141 ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
    28201 gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt
    28261 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa
    28321 cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa
    28381 cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt
    28441 atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat
    28501 ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac
    28561 cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa
    28621 gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg
    28681 cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat
    28741 ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga
    28801 ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca
    28861 aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa
    28921 gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt
    28981 taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc
    29041 gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct
    29101 catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt
    29161 atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc
    29221 atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc
    29281 gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt
    29341 cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc
    29401 gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa
    29461 catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg
    29521 atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca
    29581 tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc
    29641 gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc
    29701 gaagtgtatg ctaatccgca gcgctgcccg cagatcaatc tcattgaccg tgaagggatg
    29761 cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat
    29821 ccggaaacca cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa
    29881 tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc
    29941 gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt
    30001 catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc
    30061 ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa
    30121 gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga
    30181 tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg
    30241 cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg
    30301 ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc
    30361 tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg
    30421 agaaagcgca tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc
    30481 gtatcggtta ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta
    30541 gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca
    30601 atcccttctg tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca
    30661 ccagcgccat tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc
    30721 caggttgcct gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt
    30781 cgccgttggg gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt
    30841 gtgaaattct tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa
    30901 ccagccccga tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg
    30961 gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc
    31021 atgatgacga taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac
    31081 ggcaggagca ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc
    31141 ggcagattgg tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa
    31201 aaaaagcccg tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag
    31261 agcatatgcg tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg
    31321 cctgatccgt ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca
    31381 ggcatctgtt gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt
    31441 accgcagtac cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca
    31501 ccggctgccg ccgctttctc agccagtttc gcttccaggg catcgagggg ggatgcgcca
    31561 tcggcagaaa ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc
    31621 gccgcgaaca cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata
    31681 gttttcataa ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt
    31741 gatgagtatc acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa
    31801 ataaattgat tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg
    31861 ttttatgccg cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa
    31921 attatactcg ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct
    31981 ctgcgattat atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata
    32041 cataagcgca cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct
    32101 tcacattcat acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct
    32161 tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata
    32221 tcgtcctccc catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg
    32281 gcagttcaag cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag
    32341 cgacaggatc tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag
    32401 ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga
    32461 tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa
    32521 caccagatca cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag
    32581 ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg
    32641 catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc
    32701 attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa
    32761 gaacaatgca tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgc
    32821 gctgcgggtg tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac
    32881 gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg
    32941 cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc
    33001 ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg
    33061 tgataacggt agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa
    33121 acgtttagct ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc
    33181 cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg
    33241 ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat
    33301 aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct
    33361 ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga
    33421 gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag
    33481 taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg
    33541 gtgagcaaca ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct
    33601 ttttgcggtt taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg
    33661 aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt
    33721 tccgtcaggt ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg
    33781 tcgacggtgg tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt
    33841 ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc
    33901 ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag
    33961 gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg
    34021 ctgtcaggat gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc
    34081 tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag
    34141 aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc
    34201 gccgtgcgtg actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc
    34261 ccgcccataa tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg
    34321 cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc
    34381 gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga
    34441 ttcagggccg ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg
    34501 ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct
    34561 gtaccaaggc caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg
    34621 cgtccggcat cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatcacca
    34681 atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag
    34741 gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcctgtcggt
    34801 gcgctgcgcc cgtagtcggt gccggacggc cctttaatca cttcgacctg ctcggtattg
    34861 aaggtgtcgc gcgagacgct gccgatatcg cgaataccat caatataaat actgttagag
    34921 gtatcggcac cacgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca
    34981 aaaaacgcac ccacgccgg
//
LOCUS       DAZOYF010000023        34523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZOYF010000023 DAZOYF010000000
VERSION     DAZOYF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.023"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(207..350)
                     /gene="ypaB"
                     /locus_tag="ACHUOF_001103"
     CDS             complement(207..350)
                     /gene="ypaB"
                     /locus_tag="ACHUOF_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR0610374.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            478..2763
                     /gene="nrdA"
                     /locus_tag="ACHUOF_001104"
     CDS             478..2763
                     /gene="nrdA"
                     /locus_tag="ACHUOF_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0610375.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPNIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2997..4127
                     /gene="nrdB"
                     /locus_tag="ACHUOF_001105"
     CDS             2997..4127
                     /gene="nrdB"
                     /locus_tag="ACHUOF_001105"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0610376.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4127..4381
                     /gene="yfaE"
                     /locus_tag="ACHUOF_001106"
     CDS             4127..4381
                     /gene="yfaE"
                     /locus_tag="ACHUOF_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR0610377.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4435..5085)
                     /gene="inaA"
                     /locus_tag="ACHUOF_001107"
     CDS             complement(4435..5085)
                     /gene="inaA"
                     /locus_tag="ACHUOF_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFR0610378.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5165..5269)
                     /locus_tag="ACHUOF_001108"
                     /pseudo
     CDS             complement(5165..5269)
                     /locus_tag="ACHUOF_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5264..5506
                     /locus_tag="ACHUOF_001109"
                     /pseudo
     CDS             5264..5506
                     /locus_tag="ACHUOF_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5548..6624)
                     /gene="glpQ"
                     /locus_tag="ACHUOF_001110"
     CDS             complement(5548..6624)
                     /gene="glpQ"
                     /locus_tag="ACHUOF_001110"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0610379.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6629..7987)
                     /gene="glpT"
                     /locus_tag="ACHUOF_001111"
     CDS             complement(6629..7987)
                     /gene="glpT"
                     /locus_tag="ACHUOF_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR0610380.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8260..9888
                     /gene="glpA"
                     /locus_tag="ACHUOF_001112"
     CDS             8260..9888
                     /gene="glpA"
                     /locus_tag="ACHUOF_001112"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR0610381.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9878..11137
                     /gene="glpB"
                     /locus_tag="ACHUOF_001113"
     CDS             9878..11137
                     /gene="glpB"
                     /locus_tag="ACHUOF_001113"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR0610382.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11134..12324
                     /gene="glpC"
                     /locus_tag="ACHUOF_001114"
     CDS             11134..12324
                     /gene="glpC"
                     /locus_tag="ACHUOF_001114"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR0610383.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12517..13467
                     /locus_tag="ACHUOF_001115"
     CDS             12517..13467
                     /locus_tag="ACHUOF_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR0610384.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13508..13969)
                     /locus_tag="ACHUOF_001116"
                     /pseudo
     CDS             complement(13508..13969)
                     /locus_tag="ACHUOF_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(14033..15235)
                     /locus_tag="ACHUOF_001117"
     CDS             complement(14033..15235)
                     /locus_tag="ACHUOF_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR0610385.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15322..15864)
                     /locus_tag="ACHUOF_001118"
     CDS             complement(15322..15864)
                     /locus_tag="ACHUOF_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR0610386.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16143..16568
                     /gene="nudI"
                     /locus_tag="ACHUOF_001119"
     CDS             16143..16568
                     /gene="nudI"
                     /locus_tag="ACHUOF_001119"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR0610387.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16607..17209)
                     /gene="ais"
                     /locus_tag="ACHUOF_001120"
     CDS             complement(16607..17209)
                     /gene="ais"
                     /locus_tag="ACHUOF_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR0610388.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17517..18656
                     /gene="arnB"
                     /locus_tag="ACHUOF_001121"
     CDS             17517..18656
                     /gene="arnB"
                     /locus_tag="ACHUOF_001121"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR0610389.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18660..19628
                     /gene="arnC"
                     /locus_tag="ACHUOF_001122"
     CDS             18660..19628
                     /gene="arnC"
                     /locus_tag="ACHUOF_001122"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR0610390.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19628..21609
                     /gene="arnA"
                     /locus_tag="ACHUOF_001123"
                     /pseudo
     CDS             19628..21609
                     /gene="arnA"
                     /locus_tag="ACHUOF_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21606..22496
                     /gene="arnD"
                     /locus_tag="ACHUOF_001124"
     CDS             21606..22496
                     /gene="arnD"
                     /locus_tag="ACHUOF_001124"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR0610391.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVECCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22496..24148
                     /gene="arnT"
                     /locus_tag="ACHUOF_001125"
     CDS             22496..24148
                     /gene="arnT"
                     /locus_tag="ACHUOF_001125"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR0610392.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24145..24480
                     /gene="arnE"
                     /locus_tag="ACHUOF_001126"
     CDS             24145..24480
                     /gene="arnE"
                     /locus_tag="ACHUOF_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR0610393.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24480..24866
                     /gene="arnF"
                     /locus_tag="ACHUOF_001127"
     CDS             24480..24866
                     /gene="arnF"
                     /locus_tag="ACHUOF_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR0610394.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(24860..25132)
                     /gene="pmrD"
                     /locus_tag="ACHUOF_001128"
     CDS             complement(24860..25132)
                     /gene="pmrD"
                     /locus_tag="ACHUOF_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR0610395.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25237..26592)
                     /gene="menE"
                     /locus_tag="ACHUOF_001129"
     CDS             complement(25237..26592)
                     /gene="menE"
                     /locus_tag="ACHUOF_001129"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR0610396.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26589..27551)
                     /gene="menC"
                     /locus_tag="ACHUOF_001130"
     CDS             complement(26589..27551)
                     /gene="menC"
                     /locus_tag="ACHUOF_001130"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR0610397.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27551..28408)
                     /gene="menB"
                     /locus_tag="ACHUOF_001131"
     CDS             complement(27551..28408)
                     /gene="menB"
                     /locus_tag="ACHUOF_001131"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR0610398.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28423..29181)
                     /gene="menH"
                     /locus_tag="ACHUOF_001132"
     CDS             complement(28423..29181)
                     /gene="menH"
                     /locus_tag="ACHUOF_001132"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR0610399.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29178..30848)
                     /gene="menD"
                     /locus_tag="ACHUOF_001133"
     CDS             complement(29178..30848)
                     /gene="menD"
                     /locus_tag="ACHUOF_001133"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR0610400.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(30937..32232)
                     /gene="menF"
                     /locus_tag="ACHUOF_001134"
     CDS             complement(30937..32232)
                     /gene="menF"
                     /locus_tag="ACHUOF_001134"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR0610401.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32311..32616)
                     /gene="elaB"
                     /locus_tag="ACHUOF_001135"
     CDS             complement(32311..32616)
                     /gene="elaB"
                     /locus_tag="ACHUOF_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR0610402.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32671..33132)
                     /locus_tag="ACHUOF_001136"
     CDS             complement(32671..33132)
                     /locus_tag="ACHUOF_001136"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0610403.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33197..34114
                     /gene="rbn"
                     /locus_tag="ACHUOF_001137"
     CDS             33197..34114
                     /gene="rbn"
                     /locus_tag="ACHUOF_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR0610404.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34305..34493
                     /locus_tag="ACHUOF_001138"
                     /pseudo
     CDS             34305..34493
                     /locus_tag="ACHUOF_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8411 a         9274 c         8580 g         8258 t
ORIGIN      
        1 gtaaattaac aaaatggctt cgcatttaac aataaccgaa tagaaaacaa ccatttcgac
       61 atcaacaatc gcttacattc gcttatatat tgaccacaac tgatacatca gattatgtga
      121 tgactcgtgc ttagatcaat ttttgcaatc attagcaaaa agattaataa accatctata
      181 tcaatttatc caacctattc tgccgttcaa gaaatcgccg aacagttatt tttaacaaat
      241 ttttctcttc ccattgactt tcccggacac cttgtctgac ctaaggtgcg cgaaagccac
      301 tttttccttc ctgagttatc cacaaagtta tgcacttgca agagggtcat tttcacacta
      361 tcttgcagtg aatcccaaac atacccccta tatatagtgt tctaagcagc ttcccttact
      421 acaggtagtc tgcatgaaac tattgcggaa agaattccaa aaacaggtac gacatacatg
      481 aatcagaatc tgctggtgac aaagcgcgac ggtagcacag agcgcatcaa tctcgacaaa
      541 atccatcgcg ttctggattg ggcggcagaa ggactgcata acgtttcgat ttcccaggtc
      601 gagctgcgct cccacattca gttttatgac ggtatcaaga cctctgacat ccacgaaact
      661 attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc cggattatca gtatctcgcc
      721 gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg gccagtttga gccgcctgcg
      781 ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat acgataatca tctgctggaa
      841 gactacacgg aagaagagtt caagcagatg gacacgttta tcgatcacga ccgtgatatg
      901 accttctctt atgctgccgt taagcagctg gaaggcaaat atctagtaca gaaccgcgtg
      961 accggcgaaa tctacgaaag cgcccagttc ctttatattc ttgttgccgc gtgcctgttc
     1021 tcgaactacc cgcgtgaaac gcgcctgcaa tacgtgaagc gtttttatga cgcggtttcc
     1081 acatttaaaa tttcgctgcc gacgccaatc atgtccggcg tgcgtacccc gactcgtcag
     1141 ttcagctcct gcgtactgat cgagtgcggt gacagcctgg attccatcaa cgccacctcc
     1201 agcgcgattg tgaaatatgt ttcccagcgt gccgggatcg gcatcaacgc cggacgtatt
     1261 cgtgcgctgg gcagcccgat tcgcggtggc gaagcgttcc ataccggctg tatcccgttc
     1321 tacaaacatt tccagacagc ggtgaaatcc tgctctcagg gcggtgtgcg cggcggtgcg
     1381 gcaacgctgt tctacccgat gtggcatctg gaagtggaaa gcctgctggt gttgaaaaac
     1441 aaccgtgggg ttgaaggcaa ccgcgtgcgt catatggact acggggtaca aatcaacaaa
     1501 ctgatgtata cccgcctgct gaaaggtgaa gatatcaccc tgttcagccc gtccgacgta
     1561 ccggggctgt acgacgcgtt cttcgccgat caggaagagt ttgaacgcct gtataccaaa
     1621 tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag ccgttgagct gttctcgctg
     1681 atgatgcagg aacgtgcgtc taccggtcgt atctatattc agaacgttga ccactgtaat
     1741 acccatagtc cgtttgatcc gaacatcgcg ccagttcgcc agtctaacct gtgcctagaa
     1801 attgccctgc cgaccaaacc gctgaacgac gtcaacgacg agaacggtga aatcgcgctg
     1861 tgtacgctgt ctgctttcaa cctgggcgca attaatagcc tggatgaact ggaagagctg
     1921 gcgattctgg cggttcgtgc acttgacgcg ctactggatt atcaggatta cccgatcccg
     1981 gccgccaaac gtggagcgat ggggcgtcgt acgctgggta ttggtgtgat taacttcgct
     2041 tactatctgg cgaagcacgg taaacgctac tccgacggca gcgccaacaa cctgacgcat
     2101 aaaaccttcg aagcgattca gtattacctg ctgaaagcgt ctaatgagct ggctaaagag
     2161 caaggcgcgt gcccgtggtt taacgaaacc acttacgcga aagggatcct gccgatcgat
     2221 acctataaga aagatctgga tgccatcgct aatgagccgc tgcattacga ctgggaagca
     2281 ttgcgtgaat caatcaaaac gcacggcctg cgtaactcca cgctttctgc cctgatgccg
     2341 tccgagacct cttcgcagat ctctaacgcc actaacggta ttgaaccgcc gcgcggttac
     2401 gtcagcatca aagcgtcgaa agacggtatt ttgcgccagg tggtgccgga ctacgagcac
     2461 ctgcacgacg cctatgagct gctgtgggaa atgccgggta acgatggcta cctgcaactg
     2521 gtaggtatca tgcagaaatt tatcgatcag tcgatctctg ctaacaccaa ctacgatccg
     2581 tcacgtttcc cgtcaggaaa agtgccgatg cagcagttgc tgaaagacct gctcaccgcc
     2641 tacaaattcg gggtcaaaac gctgtattat cagaacaccc gtgacggcgc tgaagacgcg
     2701 caggacgatc tggtaccgtc aatccaggac gatggctgcg aaagcggcgc atgtaagatc
     2761 tgatattgag atgccggatg cggcgtaaac gccttatccg gcctacggct cggtttgtag
     2821 gcctgataag acgcgccagc gtcgcatcag gctccgggtg ccggatgcag cgtgaacgcc
     2881 ttatccggcc tacggctcgg atttgtaggc ctgataagac gcgccagcgt cgcatcaggc
     2941 acaggatgcg gcgtaaaatg ccttatccgg cattaaactc ccaacaggac acactcatgg
     3001 catataccac cttttcacag acgaaaaatg atcagctcaa agaaccgatg ttctttggtc
     3061 agccggtcaa cgtggctcgc tacgatcagc aaaaatatga catcttcgaa aagctgatcg
     3121 aaaagcagct ctctttcttc tggcgtccgg aagaagttga cgtctcccgc gaccgtatag
     3181 attaccaggc gctgccggag cacgaaaaac acatctttat cagcaacctg aaatatcaga
     3241 cgctgctgga ttccattcag ggtcgtagcc cgaacgtggc gctattgccg cttatttcta
     3301 ttccggaact ggaaacctgg gtcgaaacct gggcgttctc agaaacgatt cattcccgtt
     3361 cctatactca tatcattcgt aatatcgtta acgatccgtc tgttgtgttt gacgatatcg
     3421 tcaccaacga gcagatccag aaacgtgcgg aagggatctc cagctattac gatgaactga
     3481 tcgaaatgac cagctactgg caccttctgg gcgaaggcac ccacaccgtt aacggtaaaa
     3541 ccgtaaccgt tagcctgcgc gagctgaaga aaaaactgta tctctgcctg atgagcgtta
     3601 acgcgctgga agcgatccgc ttctacgtca gctttgcttg ttcctttgcc tttgcagaac
     3661 gcgaactgat ggaaggtaac gccaagatta ttcgcctgat tgcccgcgat gaagccctgc
     3721 acctgaccgg tactcagcat atgctgaatc tgctgcgcag cggcgcggat gatccggaga
     3781 tggcggaaat tgccgaagaa tgtaagcagg agtgctatga cctgtttgtc caggcggcac
     3841 agcaagagaa agactgggcg gattatctgt tccgtgacgg ttcgatgatt ggtctgaata
     3901 aagatattct ctgccagtac gttgaataca tcaccaatat ccgcatgcag gcagtcggtt
     3961 tggatctgcc gttccagacg cgctccaacc cgatcccgtg gatcaacacc tggctggtgt
     4021 ctgataacgt gcaggttgct ccgcaggaag tggaagtcag ttcttatctg gtcgggcaga
     4081 ttgacgcgga agtggacacc gacgatttga gtaacttcca gctctgatgg cccgcgttac
     4141 cctgcgcatc actggcacac aactgctgtg ccaggatgaa cacccttccc ttctggcggc
     4201 gctggaatcc cacaatgtgg cggttgagta ccagtgccgc gaaggttact gcggctcctg
     4261 tcgcacccga ctggtcgcag gtcaggtgga ctggattgcc gaaccgttag cctttattca
     4321 gccgggggaa attttgccct gttgttgccg ggcaaaaggc gatattgaaa tcgagttgtg
     4381 atcgggtact agcgccagat acgacgctta cgcgtattat ctggcctaca acgattacat
     4441 ggcgtagtaa tacgctttca cctgctccca gtccgcttta gggatcggct ccagatattt
     4501 ttccagctgg cggaagtcat gattaatcgc tttatcgcga cgcaagcggc gacgactttt
     4561 ctccagatca agaaaaccag cttctgcctt accttctgtt ttcacataga tatggcgaac
     4621 atagcaacag ccatgctgac gattaacgct atgcattttc ttaaacgcca gtgccactgc
     4681 tttcaacatg gcttgccgta cttcgtcaga ataaggcgat actgcatgcc gggcatacca
     4741 gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc agcaacgcgc gccattcacc
     4801 ctcaattttc accgcttcac caaaaacgat cttcggcacg atgacaccgg cccgttccag
     4861 ttctttaatt accgcaacct cacggacaat cgttggtcgg ccgaacggat aacgtactga
     4921 atgaaacaga tgatgcgtca tgcgctttac atacagcttt ttgccgttgc gctcgacgca
     4981 ttgcaccccg ctcataccat tacggcgata gttaggctct tcaacccagt cgccctctgt
     5041 tgcccaccag tgattaaatt cgtcgtactt tgctgaaact gccataccct atcgcctgtc
     5101 atttttatta acgacaatga ctataggtgg ttacctgagg aaaatcttaa tgaaacgtgt
     5161 cgtattaatg aatttctttt gccgctacag gtgtgacacc gcttttgccc ttcatttgca
     5221 gcgcatagat tccaccagcc actaccacta atagtgcgcc cagcagataa aatgtgaatt
     5281 taatcagtcg gatacagcga tgaattttat tcgacaggga ttaggcatcg ccttacaacc
     5341 agagttaacg ctgaaaagca ttgcaggtga attgtgttcc gttcctcacg aaccaacctt
     5401 ctatcgacag atttcgttgc tgactaaaga aaagccggta gaaggcagtc cactgttttt
     5461 actacaaatg tgcatggaac aattagtggc gattggaaaa atttgatatg agacaggatg
     5521 gcgcatgaac gccatcctgc agtaacatta ctctttatta agaaatttta ctgccttatc
     5581 agggaaatca gtaaacagcc catttacacc cgctttgtta tacagagcat catataactg
     5641 attcacatca ggagtgtatt caggcagttt atctgaccgc acggtataag gatgcactac
     5701 cagtttattc tgctgggcat cttgcaccat gccagtgagt ttgatattac ccggctgcga
     5761 tgtctcctca atcaacatat ggtaatccgg accaatacca tctgcatatt ccgccacctg
     5821 tttcatggct cccggcttaa acatccagtc gtagttgtaa ttaacccagc ttccatccgg
     5881 ctgtttctgc tgcgtttcat tccagtcggt ataggcaatc agctgtacca gattgagctc
     5941 catgcccatt ttgggttcca gctcattctt aatacgcttc agctcatcag catcaaaaca
     6001 ttgcaaataa actttatcgt ctttaccggt gtaaccatat ttcttcagca cttccagcgt
     6061 ttttgccgca atatccttcc cttcctgatg atggaaccac ggcgctttga tttctggata
     6121 gataccgata tttttcccgg tagagtgatt taacccctga acaaattcaa tctcttcttc
     6181 aaaggtgtgc acccggaagt cggacttacc cattgggaaa cgccccggat aagtctgtac
     6241 ttttttaccg ttttcaatat cgaaaccttc ggtaaatttc agcgacttaa tttcatccag
     6301 cgtgaaatct atcgcgtagt aacgaccgtc tttgcgcgcc cgatccggga aacgatcggc
     6361 aacatcagta acacgatcga gataatggtc atgcagaaca accagatggt cgtctttggt
     6421 catcaccaaa tcctgttcca gataatccgc tccctgcgca tacgccatcg cttttgctgg
     6481 cagcgtatgc tccggcaaat atccactggc accgcgatgg gcgatgacta ttttttcgtt
     6541 gctgtccgcc gccattgcac tgcttcccat gactatagtg ctcatcatga tcgccatgct
     6601 aaggtttttc agcttcaatt tcatgccatt agcctccgtt gcgtttttgc agtaattgtt
     6661 catggcgacg tttttcgcca atcatcacaa caatcaacaa gataaccgcc agaatgctgc
     6721 cgccaatcat taccataaag ccgccatccc agccgaagaa gtccacggtg tagccaacaa
     6781 tcgcgctcgc cgccaccgaa ccgcccaggt aaccaaacag cccggtaaag cccgctgccg
     6841 tacctgccgc ttttttcggt gccagttcca gcgcatgcag accgatcagc atcacaggac
     6901 cgtagatcag gaagccgata acaatcatac aaatcatatc gacggttggg ttacctgccg
     6961 ggttcatcca gtaaacgata gtcgcgatgg tcaccagtgt cataaagaag acgccggttg
     7021 ccccacggtt accacggaag actttgtccg acatccagcc gcacagcagg gtgcccggaa
     7081 tacccgcata ctcatagaag aagtaggccc aggaggattt atccagcgcg aaatgcttaa
     7141 cctctttcag ataagtcggt gaccagtcga ggatgccgta acgcagcaga taaacgaaca
     7201 cgttggcgat ggcgatgtac cacagcagtt tgttcggcag cacgtactgc atgaagattt
     7261 gcttcgcggt cagctcctgt tccgcttttt cgttatagtc gtccggataa tcatttttgt
     7321 actcttcgat cggcggcaag ccacaggatt gcggggtatc gcgcatcatc gcaaaggcga
     7381 ataatgccac cagaatggcg cagaaagcag gcatatagag cgccgcatgc cagtcattga
     7441 accaggccat ccccagcagg aacagcagcg gcggaatacc accaccgacg ttgtgcgcac
     7501 agttccacac tgacacaatg ccgccacgtt ctttctgcga ccaccagtgc accatagtac
     7561 gaccacacgg cggccacccc atcccctgga accaaccgca gaggaacaac agtacaaaca
     7621 tcaccgcaat gctcgacgtc gcccatggca caaagcccat aaacaacatc actgccgccg
     7681 ccagaatcaa acctgcgggc aggaaaacgc gcggattcga gcgatccgat accgaaccca
     7741 tgatgaattt cgaaaatcca taagcaatcg agatccccga aagggcaaaa cctaaatcac
     7801 cgcgtgagaa tccctgctca accagataag gcatagcaag cgcaaagttc ttacgaacca
     7861 aatagtaagc cgcatagcca aagaatatcc ccaggaaaat ttgccagcgc aatcgacgat
     7921 aagtcggatc gatctccgcg gcaggtaagc gcgctttgtg tggcgctggt ttaaaaatac
     7981 tcaacattga tagcctccgt ggcccgtggt cttatttatg attaacagcc tgattcagtg
     8041 agagaacctg ccgtttcttg agttgccgcg atgttaagaa aacattcata aattaaatgt
     8101 gaattgccgc acacattatt aaataagatt tacaaaatgt tcaaaatgac gcatgaaatc
     8161 acgttttact ttcgaatcat gagcgaatat gcgcgaaatc aaacaattca tgtttttact
     8221 atggctaaat ggtaaaaaac gaacttcaga gggataacaa tgaaaactcg cgactcgcaa
     8281 tcaagtgacg tgattatcat tggcggcggc gcaacgggag ccgggattgc ccgcgactgt
     8341 gccctgcgcg ggctgcgcgt gattttggtt gagcgccacg acatcgcaac cggtgccacc
     8401 gggcgtaacc acggcctgct gcacagcggt gcgcgctatg cagtaaccga tgcggaatcg
     8461 gcccgcgaat gcattagtga aaaccagatc ctgaaacgca ttgcacgtca ctgcgttgaa
     8521 ccaaccaacg gcctgtttat caccctgccg gaagatgacc tctcattcca ggccactttt
     8581 attcgcgcct gcgaagaagc agggatcagc gcagaagcta tagacccgca gcaagcgcgc
     8641 attatcgaac ctgccgttaa cccggcactg attggcgcgg tgaaagttcc ggatggcacc
     8701 gttgatccat ttcgtctgac cgcagcaaac atgctggatg ccaaagaaca cggtgccgtt
     8761 atccttaccg ctcatgaagt cacggggctg attcgtgaag gcgcgacggt gtgcggtgtt
     8821 cgtgtacgta accatctcac cggcgaaact caggcccttc atgcacctgt cgtggttaat
     8881 gccgctggga tctgggggca acacattgcc gaatatgccg atctgcgcat tcgcatgttc
     8941 ccggcgaaag gatcgctgct gatcatggat caccgcatta accagcatgt gatcaaccgc
     9001 tgccgtaaac cttccgacgc cgatattctg gtgcctggcg ataccatttc gctgattggt
     9061 accacctctt tacgtattga ttacaacgag attgacgata atcgagtgac ggcagaagag
     9121 gttgatattc tgctgcgtga aggggaaaaa ctggcccccg tgatggcgaa aacgcgcatt
     9181 ttgcgggcct attctggcgt gcgcccgctg gttgccagcg atgacgaccc gagcggacgt
     9241 aacgtcagcc gtggcatcgt gctgctcgac catgctgaac gcgatggtct ggacggattt
     9301 atcaccatca ccggtggcaa actgatgacc tatcggctga tggctgaatg ggctaccgac
     9361 gcggtatgcc gcaaactggg caacacgcgc ccctgtacga ctgccgatct ggcactgcct
     9421 ggttcacaag aacccgctga agttaccttg cgtaaagtca tctccctgcc tgccccgctg
     9481 cgcggttctg cggtttatcg tcatggcgat cgcacgcctg cctggctgag cgaaggccgt
     9541 ctacaccgta gcctggtatg tgagtgcgaa gcggtaactg cgggtgaagt gcagtacgcg
     9601 gtagaaaatt taaacgttaa tagcctgctg gatttacgcc gtcgtacccg tgtggggatg
     9661 ggcacctgcc agggcgaact ctgcgcctgc cgcgctgccg gactgctgca acggtttaac
     9721 gtcacgacgt ccgcgcaatc tatcgagcaa ctttccacct tccttaacga acgctggaaa
     9781 ggcgtgcaac ccatcgcctg gggagacgca ctgcgcgaaa gcgaatttac ccgctgggtt
     9841 tatcagggac tgtgtggtct ggagaaggag cagaaagatg cgctttgata ctgtcattat
     9901 gggcggcggc ctcgccggat tactctgtgg cctgcaactg caaaaacacg gcctgcgctg
     9961 tgccattgtc acgcgtggtc aaagcgcgtt gcatttctca tccggttcgc tggatttgct
    10021 gagccatctg ccagatggtc aacccgtggc agacattcac agtggactgg aatctttgcg
    10081 tcagcaggca cctgcacatc cttactctct tctcgggcca caacgcgtgc tcgatctcgc
    10141 ttgccaggcg caggcattaa tcgctgaaag cggtgcgcaa ttgcagggca gcgtagaact
    10201 ggctcaccag cggattacgc cgctcggcac tttgcgtgca acctggttaa gttcgccaga
    10261 agttcccgtc tggccgctac ccgcgaagaa gatatgtgta gtggggataa gcgggctgat
    10321 ggatttccag gcgcatcttg cggcggcttc gttgcgcgaa ctggatcttt cggttgaaac
    10381 cgcagaaata gagctgccgg aactggatgt cctgcgcaat aacgccaccg aatttcgcgc
    10441 ggtgaatatc gcccgtttcc ttgataatga agaaaactgg ccgctgttac ttgatgcgct
    10501 tattcctgtc gccaatacct gcgaaatgat cctgatgccc gcctgcttcg gcctggccga
    10561 tgacaaactg tggcgttggt tgaatgaaaa actaccttgt tcactgatgc ttttgccaac
    10621 gctgccgcct tccgtgctgg gcattcgtct gcaaaaccag ttacagcgcc agtttgtgcg
    10681 ccaaggtgga gtgtggatgc cgggcgatga agtgaaaaaa gtgacctgta aaaatggcgt
    10741 agtgaatgaa atctggaccc gcaatcacgc cgatattccg ctacgtccac gtttcgcggt
    10801 tctcgccagc ggcagtttct ttagtggcgg actagtagcg gaacgtaacg gcattcgcga
    10861 gccgattctc ggccttgatg tgctacaaac cgccacgcgg ggtgaatggt ataagggcga
    10921 tttttttgcg ccgcaaccgt ggcagcagtt cggtgtaacc actgatgaga cgctacgccc
    10981 gtcgcaggca gggcaaacca ttgagaacct gtttgccatc ggttcggtgc tgggcggatt
    11041 tgatcccatc gcccagggat gcggcggcgg tgtttgtgcc gtcagtgctt tacatgccgc
    11101 tcaacagatt gcccaacgcg caggaggcca acaatgaacg acaccagctt cgaaaactgc
    11161 attaagtgca ccgtctgcac caccgcctgc ccggtgagcc gggtgaatcc cggttatcca
    11221 gggccaaaac aagccgggcc ggatggcgag cgtctgcgtt tgaaagatgg cgcactgtat
    11281 gacgaggcgc tgaaatattg catcaactgc aaacgttgtg aagtcgcctg cccgtccgat
    11341 gtgaagattg gcgatattat ccagcgcgcg cgggcgaaat atgacaccac gcgcccgtcg
    11401 ctgcgtaatt ttgtgttgag tcataccgac ctgatgggta gcgtttccac gccgttcgca
    11461 ccaatcgtca acaccgctac ctcgctgaaa ccggtgcggc agctgcttga tgcggcgtta
    11521 aaaatcgatc atcgtcgcac gctaccgaaa tactccttcg gcacgttccg tcgctggtat
    11581 cgcagcatag cggctcagca agcacaatat aaagaccagg tcgctttctt tcacggctgc
    11641 ttcgttaact acaaccatcc gcagttaggt aaagatttaa ttaaagtgct caacgcaatg
    11701 ggtaccggtg tacaactgct cagcaaagaa aaatgctgcg gcgtaccgct aatcgccaac
    11761 ggctttaccg ataaagcacg caaacaggca attacgaatg tagagtcgat ccgcgaagct
    11821 gtgggagtaa aaggcattcc ggtgattgcc acctcctcaa cctgtacatt tgccctgcgc
    11881 gacgaatacc cggaagtgct gaatgtcgac aacaaaggct tgcgcgatca tatcgaactg
    11941 gcaacccgct ggctgtggcg caagctggac gaaggcaaaa cgttaccgct gaaaccgctg
    12001 ccgctgaaag tggtttatca cactccgtgc catatggaaa aaatgggctg gacgctctac
    12061 accctggagc tgttgcgtaa catcccgggg cttgagttaa cggtgctgga ttcccagtgc
    12121 tgcggtattg cgggtactta cggtttcaaa aaagagaact accccacctc acaagccatc
    12181 ggcgcaccac tgttccgcca gatagaagaa agcggcgcag atctggtggt caccgactgc
    12241 gaaacctgta aatggcagat tgagatgtcc acaagtcttc gctgcgaaca tccgattacg
    12301 ctactggccc aggcgctggc ttaaactccc ttctgatgcc cggtaagcat gtggttaccg
    12361 ggcatttttg cgtacacgat tccgtgccca atgtatgcgt tgcaacgcag tgaaaattcc
    12421 tctgaaaacg tctcgcaaag gctgaaactg gcagatgtca aaggcctggg ataaccgtaa
    12481 tgtcgcgtca tcataaatat caggtgacgg acaaccatga ccgaatcaac aacctcctcc
    12541 ccgcatgatg cggtatttaa aacctttata ttcacacccg aaaccgcacg ggattttctc
    12601 gaaatacatt taccagaacc actgcgcaag ctttgcaacc tgcaaacctt acgcctggaa
    12661 cccactagtt ttattgaaaa aagtttacgc gcttactact cggatgtttt gtggtccgtg
    12721 gaaaccagcg acggtgacgg ctatatctac tgcgtgattg aacatcaaag ctctgcagaa
    12781 aagaatatgg cttttcggcc aatgcgctat gccactgccg ccatgcagag tcacctagat
    12841 aaaggctatg acagagttcc gctggtggtg ccattgctgt tttatcatgg cgaaacatcg
    12901 ccctacccgt actcacttaa ctggctggat gagtttgacg atccgcaact tgcccggcag
    12961 ttgtacaccg aagcttttcc gttggtggat atcaccatcg tacctgacga tgagatcatg
    13021 caacatcggc gtatagctct gctggaactg attcaaaagc atattcgcga ccacgattta
    13081 atcggcatgg tcgacaggat caccacgctt ttggttagag gcttcactaa tgacagccag
    13141 ctacaaacac tgtttaatta tctgctgcaa tgcggcgata cctcccgttt cacccgtttt
    13201 attcaggaga ttgccgaacg ttcaccacta caaaaggaga gattaatgac tattgctgaa
    13261 cggctacggc aggaaggaca tcaaattggc tggcaagaag gtaaattagt aggtttgcag
    13321 caaggtaaat tagaaggttt gcaggaaggt atgcatgaac aagccattaa aattgctttg
    13381 cgcatgctgg aacagggcat tgatcgtgac caagtgctcg cggccaccca gctaagcgaa
    13441 gccgatctgg cagcgaataa ccactaatta acacaggccc acagccgatc cccatgggcc
    13501 tttgatatca ataactacct tttatgcgtg gcccattttt gcctgattta aacatcgcca
    13561 gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac gccaacagcg acaaagttcg
    13621 ctccccacgc caggcattgc tgcgccatat caggagccac agccagaaaa ccagccgctt
    13681 tacccgcagc acggatccgc cgaatgctgg tttcaataat tcgctgcact tccgggtgcc
    13741 cggcgttatc cgggtagccc aacgacgcag aaagatccgc tggtccaata aacacgccat
    13801 caatcccttc gacgtcgagg atctcgtcca ggttatccag tgccgtttta ctttccacct
    13861 gcactaacag acaaagcgaa tcgttgactt gcgccatgta attctcaatg cgtccccagc
    13921 gcgcagcccg tgccacactg gccccaacac cacgctcacc gtagggaggg ttggacaaat
    13981 tttgccggag ggggaatgtg ccggtgcagt aaaccggcac agagtggcga aatcagacgg
    14041 ataaagtcat ggactcaaca acctcaatcc agccatgctc actagcgatg tcctggccgt
    14101 ttaaccaacg gcgtagcata ttcagtgcca tcattgcgca cacttcctga cggatagcca
    14161 ggctgtagcg tgtagtgctg aaacgcacac gcagagcgaa agtgccgtct ggcgtggcta
    14221 gcgcaaagtt gagatgctcg ttctcgaaac ccgaaacagc cagtgccagc ccggcaaaat
    14281 ggttggcccg ccgttctgta atccagtgcg cagtttgcgc cagggtttct tcctgtgaag
    14341 gaatcacttc acacgccagc aatggagcac ctgcgcgaga aagttgcaaa gccaataaac
    14401 caccggtgaa ttgctcgctc aacgtcaggc tgaactggcg gttttgcaat tcgcgactga
    14461 tctgcgcggg cagtccttcg gtgccttcga aaatcacgct ctgtccggca acacgtttaa
    14521 cgtccagcca cagtttttcc atcgcctgtt gctcgcttgc cggtccggtg agtttcagtt
    14581 cgatgatcgg cattgaggag cgatagccca ttgttacgcc cggcggcagt tgtagagtgt
    14641 ccaggctttg tgccagatcg ctttccgaac gaccaaaggt agttaaacgc agacaaaccg
    14701 gcggctgtgg taaagaaaaa cgctcgcgca ggcgcggcag gatttcgtgc tcgaccatca
    14761 ccttaaattc tgacggtacg ccgggagtaa agaacatcag gcaacgatta agctgcacgg
    14821 caaaaccaca ggcggtgcct accgggttat tgataaattc agcactggca ggcagctccg
    14881 cttgtttacg gttgctcggt gccattactc gtccacgttc gtggaaatag cgttccatct
    14941 ctttgagcca ggcttcatgc agcaccaggc cttcaccttt tgctgtcgca gcggcgagtg
    15001 cgcttaaatc atcgctggtc ggtcccagcc cgccgttaac gatcagcaca tcggcgtgct
    15061 gactacgttc gcgaagaatg gtgactaagt catcaaggtt atcccccacc gtattgcggc
    15121 gagataatgg cagcccctga tgaaagaaaa aatcggccag ccaggcagcg ttagtgtcaa
    15181 cgatttgccc gtataacact tcatccccgg tggatagcat ttccactttt aacatggtta
    15241 tctcctgctt ttgttatcga atcactataa cgcaaaggga gataaggcgt agtaagaaac
    15301 tgaatgccgc gccggaaggg atcagaaact ggcgttaacc ccaacataca ggccgtcagc
    15361 cacagcgttg tcgcggttac cgtctttacc cgacagattc aggtagcgat aacccgcctc
    15421 aatactgact ggacgcataa tggtgtaacg cgcgccagca ttcgcttcct cataactttg
    15481 aataccgctg gagagcgaat ccggagagta gtaatactcg ccaaacaaac ggaagctgtt
    15541 gccaattttc cactgcaaac cacctcctac cgctgcggca taaccttcat cgccgtaatt
    15601 cgggttggtg tacacgcctt ttccgccaac ggtcgccatt aacggcccga gaggcagatt
    15661 caaccccagc cccacgcccg cgacgtcacc gtcgtcgtcg ttatgtgtcc agttaccgct
    15721 taaagccagg cccgtcgatt cagtcccaaa accgacacca atattggtgt attctttacc
    15781 cgcctggccg ctgatgctca ttgcattgac cgatgcagaa accaacagca ttccggccag
    15841 acctgctagc gcaatttttt tcattatccc ctttcccaca agcaattaat caaaattccc
    15901 aaagctgcgg gattgtactg cctggcacag aggattcaat gagcgaaaaa agggatctag
    15961 tcacttattg taactaaata cttcttaaat aattaaacgc cgaaaaaata aacacacttt
    16021 accgatacac acaaaaataa taataagtgc agttatcatt gcaaaagaga ttactgtcac
    16081 tttcacatat ttctgagtta ccgtattctc atggtttctt tttatttata aggaataccc
    16141 ctgtgcgaca acggactatt gtatgccctt tgattcaaaa tgatggtgct tatttgctgt
    16201 gcaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg ggcgctttcg ggtggcggcg
    16261 tggagtctgg cgaacgaatt gaagaggcac tacgccgcga aattcgcgaa gaactgggag
    16321 aacagctgct tttgacagaa atcacgccgt ggaccttcag cgatgatatt cgcaccaaga
    16381 catatgcaga tggtcgcaag gaagagattt atatgattta cctgattttt gactgcgttt
    16441 ctgccaaccg agaagtgaaa ataaacgaag agtttcagga ctacgcgtgg gtaaaacctg
    16501 aagatctggt gcattatgat ttaaatgttg ccacccgaaa aacgttacgt ttgaaaggtc
    16561 ttctttaaca atgatgacag ccatcactgg ctgtcagatt gaaaatttag tggttaacga
    16621 attccccatc cagataaact ttgccttttt ccacatgcat gactaaacca tccagataat
    16681 caggtttaaa tatcgcatca cgcttatttt tagcaatata tgtcaggcaa tgattatggg
    16741 tgaaaataac gatattttta tcaggcgctt tgctttgtaa gtccttaatt gcactataaa
    16801 tctcattacc gcactgaaga agtcgtttat ctaccgtcag ctttttaccc gcggaaaacc
    16861 aggtcgccga ctgaatggtc cggacggtat tactggaata aagatcgaaa tcagagatgt
    16921 cagcgctaaa agcgttgccc agttcacggg catcctgggt acctttaacc gtaatacctg
    16981 ttttatctga caagcattga ttggttgaac gatcgcaacg ttcagcatgg cgaaacaaaa
    17041 cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt attgtcgata cgtggcaaac
    17101 cattgctact ccaggcggca tgagtaccca gtccggcaat tgcagcgagc gccagtaaaa
    17161 tgatgaaata tttttttgac ttcaatgatg agcggcaaaa agctaacacg taaactccac
    17221 ctatagacaa gcgcaaccag acaattaccg tgaaattgag cgacatttct ggcgataatt
    17281 agcagttggt gtaatattaa aaatcctacg atgtcggcaa aatgcctcaa atttttgcca
    17341 aatgcaaagc ctaaataaga aaaaatataa aaatttcaat atttacgtct aatattagtt
    17401 tcttaaggtt aagttaatat tctatcctta aaatttcgct ccaaatggca aaatatacac
    17461 aacactcttt atagcaaata taagtggaca ggtattcaat ggcggaagga aaagcaatgt
    17521 cagaattttt gcctttttcg cgaccagcaa tgggcgtgga ggaactcgct gcagttaaag
    17581 aggttctcga atccggttgg atcacaaccg gtccgaagaa tcaggcgctt gagcaagcct
    17641 tttgccagtt gacgggaaat cagcatgcca tcgcggtcag ttcagccacc gccggaatgc
    17701 atatcacgct aatggcgttg gaaattggca agggcgatga agtgattacg ccttccctga
    17761 cctgggtttc aaccctcaat atgatttcct tgctgggtgc aacgccggta atggtggatg
    17821 tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga atcagccatt acgccacgca
    17881 ctaaagccat cattccggtg cattatgccg gtgcgccagc agatattgac gccattcgcg
    17941 ccattggcga acgttacggc atcgcagtta tcgaagatgc tgcccatgcc gtcggtacgt
    18001 attacaaagg gcgacatatt ggcgcaaaag gtaccgctat tttttcattt catgccatta
    18061 aaaatattac ctgtgctgaa ggtggcctga ttgtaactga taatgaaaac cttgcccgcc
    18121 agctacggat gctgaaattt cacggtctgg gtgtcgatgc ctatgacaga caaacctggg
    18181 gccgtgcacc gcaggctgaa gtcttaacac cgggctataa gtacaatctg accgatatta
    18241 acgccgcgat tgccctgaca cagttagtca aattagagca cctcaacacc cgtcggcgcg
    18301 aaattgccca gcaatatcag caagcactgg cagctctccc ctttcagcca ttaagccttc
    18361 ccgcctggcc gcacgttcac gcctggcatc tgtttattat tcgtgtcgat gaacaacgtt
    18421 gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga aagaggcatt ggtaccgggt
    18481 tacatttccg cgccgctcac acacaaaaat attatcgcga gcgttttccc acgctgtcgt
    18541 taccgaatac cgaatggaat agcgaacgca tctgttcgtt gccgctgttc ccggatatga
    18601 ctaccgccga tgccgaccgc gtcatcacag cccttcagca actcgcagga caataagcca
    18661 tgtttgaaat ccaccctgtt aagaaagtct cggtggttat tcccgtttat aacgagcagg
    18721 aaagcttacc ggaattaatc aggcgcacca ccacagcctg tgaatcgttg gggaaagagt
    18781 atgagatcct gctgattgat gacggcagta gcgataattc cgcgcatatg ctggtcgaag
    18841 cctcacaagc ggagaacagc catattgtgt ctattttgat taaccgcaat tacgggcaac
    18901 attcagcgat tatggcggga ttcagtcacg ttactggcga cttaattatt acccttgatg
    18961 ccgatctcca gaatccgcca gaagaaatcc cccgcctggt ggcaaaagcc gatgaaggtt
    19021 acgacgtggt agggactgta cgccagaacc gccaggacag ctggtttcgt aaaacagctt
    19081 cgaagatgat taaccggctt attcagcgca ccactggcaa agcgatgggt gactacggtt
    19141 gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat gttgcactgc catgaacgca
    19201 gcacctttat cccgattctg gcgaatatct tcgcccgccg tgcaattgaa attccagtac
    19261 atcatgccga gcgtgagttt ggtgaatcca aatacagttt tatgcgcctg attaatttga
    19321 tgtacgacct ggtgacctgc cttaccacaa cgccgctacg tatgctgagt ctgctcggca
    19381 gcattattgc gattggaggt tttagcattg cggtgctgct ggtgatttta cgcctgacct
    19441 tcggaccaca atgggcggca gaaggcgtct ttatgctatt tgccgtgctg tttactttta
    19501 ttggcgctca gtttatcggc atgggattac tcggtgaata tatcggcagg atctacaccg
    19561 atgtccgcgc ccgcccccgc tattttgttc agcaagttat ccgtccatcc agcaaggaaa
    19621 atgaataatg aaaaccgtcg tttttgccta ccacgatatg ggatgcctcg gtattgaagc
    19681 tctgctggct gccggttacg aaattagcgc catttttacc cataccgata atcccggtga
    19741 aaaagccttt tatggttcgg tggctcatct ggcggcggaa agagacattc cggtttatgc
    19801 gccggataac gttaatcatc cgctgtgggt ggaacgcatt gcccaactgt caccagaggt
    19861 gattttctct ttttattatc gccatcttat ttgcgacgag atttttcagc tcgctcccgc
    19921 aggtgcattt aatctgcacg gttcgctgtt accaaaatat cgtggtcgcg cgccgctgaa
    19981 ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca ttgcaccgaa tggtaaacgt
    20041 gccgatgctg gggccattgt ggcgcaactg cgcgttgcca ttgcgccaga cgatatcgct
    20101 attacgctgc atcataaatt gtgccatgcc gcgcgccagc tactggaaca gacattaccc
    20161 gccattaaac acggtaatat tctggaaatc gcccagcgcg aaaacgaagc cacctgtttt
    20221 ggtcgcagaa cgccggatga cagtttcctt gaatggcata aaccggcatc cgtactgcac
    20281 aacatggtac gtgccgttgc cgatccgtgg ccgggtgcct tcagctatgt tggcaatcag
    20341 aaattcaccg tctggtcgtc gcgtgttcat cctcatgcca gcaaagcaca gccggggagc
    20401 gtgatttctg ttgcgccact gctgattgcc tgtggcgatg gcgcgctgga aatcgtcacc
    20461 ggacaggcgg gcgacggcat tactatgcag ggctcgcaat tagcgcagat gctgggcctg
    20521 gtgcaaggtt cacgcttgaa tagccagcct gcctgcaccg cccgacgccg tacccgggta
    20581 ctcatcctcg gggtgaatgg ctttattggc aaccatctga cagaacgcct gctgcgcgaa
    20641 gatcattatg aagtttacgg tctggatatt ggcagcgatg cgataagccg ttttctgaat
    20701 catccacatt ttcactttgt cgaaggcgat atcagtattc attccgaatg gattgagtat
    20761 cacgtcaaaa aatgtgatgt cgtcttgccg ctggtggcga tagccacgcc gattgaatat
    20821 acccgcaacc cgctgcgcgt atttgaactc gattttgaag agaatctgcg cattatccgc
    20881 tactgcgtta agtaccgtaa gcgaatcatc ttcccgtcaa cttcagaagt ttatgggatg
    20941 tgtagcgata aatacttcga tgaggaccat tctaatttaa tcgtcggccc ggtgaataaa
    21001 ccacgctgga tttattcggt gtcaaaacaa ttacttgatc gggtgatctg ggcctatggc
    21061 gaaaaagagg gtttacagtt caccctcttc ctcccgttta actggatggg accacgactg
    21121 gataacctta atgcagcgcg aattggcagc tcccgcgcta ttacgcaact cattctcaat
    21181 ctggtagaag gttcaccaat taagctaatt gatggcggaa aacaaaaacg ctgctttact
    21241 gatatccggg acggtatcga ggcgttgtac cacattatcg aaaatgcggg aaatcgctgc
    21301 gacggcgaaa ttatcaacat tggcaatcct gagaacgaag cgagcattga ggaactgggc
    21361 gagatgctgc tggcgagctt cgaaaaacat ccgctgcgcc atcatttccc accgtttgcg
    21421 ggctttcgcg ttgtcgaaag tagctgctac tacggcaaag gatatcagga cgtagagcat
    21481 cgtaaaccga gcatccgcaa tgcccaccgc tgcctggact gggagccgaa aattgatatg
    21541 caggaaacca tcgacgaaac gctggatttc ttcctgcgca ccgttgatct tacggataaa
    21601 ccatcatgac caaagtaggc ttacgcattg atgtcgatgc ctttcgtggc acccgtgaag
    21661 gcgtgccgcg tctgctggaa atcttgagta agcataatat tcaggccagc atttttttca
    21721 gcgtcggccc ggacaatatg ggccgccatc tctggcgact ggtgaagcca cagtttttgt
    21781 ggaagatgct gcgctcaaac gcggcatcgc tttatggctg ggatatttta ctggcaggta
    21841 cggcctggcc aggtaaagag attggtcatg ccaatgccga tatcattcgt gaagcggcta
    21901 aacatcacga agtcggtctg cacgcctggg atcaccatgc ctggcaagcc cgtagcggta
    21961 actgggatcg gcaaacaatg atcgacgata ttgcacgcgg tcttcgcact ctggaagaga
    22021 ttatcggtca accggtaacc tgttctgccg ctgcgggctg gcgtgccgac cagaaggtga
    22081 tcgaagcaaa agaagcgttc catttgcgct acaacagcga ttgtcgtggg gccataccgt
    22141 tccgtccact gcttgaatca ggaaaccctg gcactgcgca aattccggtg accttaccca
    22201 cctgggatga agtgattggt cgggatgtga aagcagaaga ttttaacggt tggttactca
    22261 accgcatcct gcgagataaa ggcacgccgg tttataccat tcatgcagaa gttgaatgct
    22321 gcgcttatca gcataatttt gtggatctcc tcaaacgcgc agctcaggaa ggcgtgacat
    22381 tttgcccttt aagcgaactg ttatcagaga cgttgccgct cggacaagtt gttcgcggaa
    22441 atattgccgg acgtgaaggc tggctgggtt gccaacaaat tgcgggtagt cgctgatgaa
    22501 atcggtacgt taccttatcg gcatcttcgc gtttattgcc tgctattacc tgttaccgat
    22561 cagcacgcgt ctgctctggc aacccgatga aacgcgttat gcggaaatca gtcgagaaat
    22621 gctggcatcc ggcgactgga ttgtgcccca tctgttaggg ctacgttatt tcgaaaaacc
    22681 cattgccgga tactggatta acagcattgg gcaatggcta tttggcgcga ataactttgg
    22741 tgtgcgggca ggcgttatct ttgcgaccct gttaactgcc gcgctggtga cctggtttac
    22801 tctgcgctta tggcgcgata aacgtctggc tctactcgcc acagtaattt atctctcatt
    22861 gtttattgtc tatgccatcg gcacttatgc cgtgctcgat ccgtttattg cattctggct
    22921 ggtggcggga atgtgcagct tctggctggc aatgcaggca cagacgtgga aaggcaaaag
    22981 cgcaggattt ttactgctgg gaatcacctg cggcatgggg gtgatgacca aaggttttct
    23041 cgcccttgcc gtgccggtat taagcgtgct gccatgggta gcgacgcaaa aacgctggaa
    23101 agatctcttt atttacggct ggctggcggt tatcagttgc gtactgacgg ttctcccctg
    23161 gggactggcg atagcgcagc gggagcctga cttctggcac tattttttct gggttgagca
    23221 tattcaacgc tttgcactag atgatgccca acatagagct ccgttctggt actacgtgcc
    23281 ggtcatcatt gccggtagcc tgccgtggct gggattactc cccggtgcac tgtacacagg
    23341 ctggaaaaac cgcaagcatt ccgcaaccgt ctatttgttg agctggacga taatgccgct
    23401 gctgtttttc tccgtcgcta aaggcaaatt gcccacctat attctttctt gctttgcacc
    23461 tctggcaatg ctgttggcgc attacgcgtt gctggcagca aaaaataatc ctctggcgct
    23521 gcggattaat ggctggatta acatcgcttt tggcgtcact ggcattattg ccacatttgt
    23581 gatctccccc tggggaccaa tgaacacgcc ggtgtggcaa accttcgaga gctataaagt
    23641 cttttgtgcc tggtcgattt tttcgctatg ggcatttttc ggctggtaca ccttaacaaa
    23701 cgtcgaaaag acctggtctt tcgccgcgct ttgcccgctg gggctggcgt tgctggtagg
    23761 attttcaatt cctgacagag tcatggaagg aaaacatccg caattttttg tcgagatgac
    23821 acaagaatca cttcagccaa gccgctatat tcttactgat agcgtcggtg ttgccgcagg
    23881 tctggcatgg agcctgcaac gcgatgacat catcatgtat cgccagacag gtgagttgaa
    23941 atacggcctt aattatccgg atgcgaaagg gagatttgtc agcggtgatg agttcgcaaa
    24001 ctggcttaat caacatcgtc aggaggggat tattacactc gtgctttcgg ttgaccgcga
    24061 tgaagatatc aacagtctcg ccattccgcc cgcagatgcc atcgttcgtc aggagcgtct
    24121 ggtgctgatt cagtatcgtc ccaaatgatc tggctaacat tagtctttgc cagcttgctt
    24181 agcgttgccg ggcagttgtg tcagaaacag gcaacctgct ttgtggcgat aaacaaacgg
    24241 cgcaaacata tcgtgctgtg gctgggactg gcgctggctt gtcttggtct tgccatgatg
    24301 ctctggctgc tggtcttgca gaacgtaccg gtaggcattg cctacccgat gttaagtctg
    24361 aattttgtct gggtgacgct ggctgcagta aaactgtggc acgaaccggt atcgccgcgt
    24421 cactggtgtg gggtggcgtt cattattggt ggcattgtga tcctcgggag tacggtgtaa
    24481 tgtgcctgat atggggatta ttcagcgtca ttattgcctc agttgcgcaa ttaagcctgg
    24541 gttttgcggc aagtcatctg ccgccgatga cgcacttatg ggattttatc gcggctctgt
    24601 tagcttttgg cctggatgcc cggatactgt tactcgggct gctgggttat ctgctttccg
    24661 ttttttgttg gtataaaacg ctacataaac ttgcgctaag caaagcctat gccttgctca
    24721 gtatgagtta tgtgctggta tggattgctt caatggtact tcccggctgg gaagggactt
    24781 tttcgctgaa agcactactg ggagtagcct gtattatgag cgggttgatg ctgattttcc
    24841 tgcccacgac aaaacaacgt tactgagttt tccctgccgc tgtacactgc cgttcccact
    24901 catcggggct ataactgctt gccgagagga ctttcaccgt atgcagcttt tcacgattaa
    24961 tacaataaag cgcattctgc aaaggcgaga gtaaatcccc cactttcacg gcgaagtcac
    25021 tcttcacttc ggcgatcatt tttatcgcgc cgccagcatc gcagagcata agtacatgtc
    25081 tgttatcttg tttattgcag cacgattttt tgtgccagcc attccattgc attatcctgt
    25141 ttgctaagag ttttcacatc aatttacaga ttaacagata aactctttat caacggcaat
    25201 ggaatggcag aaatgagaag tgaaacggag ggttaattat tgctgacgtt gcacccactc
    25261 ttttagcgcc tgacgtgaaa ttttaatacc gccgtttttc agctccggcg gtaaagtcag
    25321 ccagcgcacc ggttgttgaa aacgagccag cttatctttc acccattcgc caagatcaac
    25381 ggtttgctgg tcatactcca ccaccgctac cggccgatga ccaaactcct tgtcggcaac
    25441 ggaaacgata aacacctgca aaaccgcagg atgtgcagca ataacacgct cgacttcttc
    25501 cggctgaata ccctcaccgc cactgaagaa cagattgtct aaacgtccga caatggtcag
    25561 cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac cagccttcgt cattaaccag
    25621 tgaaaccagt tgcccgttac gccagtaacc ttctgccata ctggcagccc gcagccacac
    25681 ttcattatta acgattttca cttcccgacc cggcagcggc aaaccaacgt ctgccaggcc
    25741 gtcggcttct ttcgcacata ccgtggaggc aaactcggtc agaccatagc cgcaaaagca
    25801 acgaatcccc tgctcgcgcg cctgttccgt caactcgacc gggatagccg cgccgccaag
    25861 taacaccgct ttcagggaaa cggaactgcg gttaaccagc aaacgccaga gttgtgttgg
    25921 caccagtgaa gcgtgagtac agcctgccag catttgctcc aatggctgtt tatcacgcac
    25981 cgtcatccgc gcaccagcgt ataaccagcg ccacataatt ccctgaccgg agacgtgaaa
    26041 cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac ggaatcagcg ataacacacc
    26101 ttgcgcactg gcaagatggg cctgataagt atggacagcg gctttcggca aaccggtaga
    26161 acccgaggtc aacgtcattg agcacagacg cgtcggctgc cacgcagcgg catgtacgcc
    26221 ttcaaccagc tgaatgtgca gcgacgttaa tgccggaaac gtgttttccc catccggcac
    26281 cagcgcaaat tgcagcgtca gattgggcag caattcttca agcaacggtt gcggcagctg
    26341 agggttcacg ggcaacaccc gcgccccgca ttgcagtaac gccagccagg cgagcagcgt
    26401 ttgcggcgta ttccacgccc gcaacatcac gccgctgccc tcaaccaccc cctgcgccgc
    26461 aaagcccgta gctaactcat cgacgcgagc acaaagctcg cgccagttga gttgctcgtc
    26521 attaagacgt aaggcgatgg cttctccccg cacttgccgc cagtgacgcc acggccagtc
    26581 agagaagatc ataacaactg ctcctgtaca tcagcgtcca ctaacggcag tgggctcccc
    26641 ggccagcgac gaacctgctg cgcctgcatc agatccagcg tgtccagtcc cggaatggtg
    26701 tctggcgtta accaggcagc aatccgcgcc agttgcgtta gacctaagct cgactcaatg
    26761 gaagaactga tcaccgccgt cagccccagc gcatgcgccg cctgtacctg ctcgcgtact
    26821 ttttccagac tgcccgtgag cgtgggtttg ataactaccg cgcgcacgcc ctcttcagcc
    26881 acaaaggcaa aatccggctc gcgcaggctt tcatcccagg caatggcaat gccggtttca
    26941 cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct cttcgagaaa cgcgatgcgg
    27001 tggcgataat ccgggttaac atatttggca aactgctgac ctttcagcgg tgtccaggcg
    27061 cgatttgcgt caagacgcaa atgcagatcc ggaattgcct ccagcaacag attcaccacc
    27121 atgccgtcgc gcaccgcttc gtacaatccc actttgacct tcgccacttt ctcgcccggc
    27181 atatcggcaa gtttgaggat cagatcgtcc ggatcgccat tacacagcgg tgccgcacgg
    27241 tagttggctg cttgcggcaa cgtatctgtc agttctgcca atgcacagct tacgccaaag
    27301 gccacggaag gcatctgcgg tagctcgcaa tcgcctgcca gccagttatt tacccaggca
    27361 agcagcacgc tttgcgcctc ttcccagttt tcctggctga agcccggcag tggggagatc
    27421 tccccccacc cttcgcgctc gccctcacgc aggcaaacgt acagcccgtc gcgggttttt
    27481 aaccgcctgt cgcgcagaac cacccccgcg tccatgggga tctgccagcg gtatacctgc
    27541 gcgctacgca ttacggattc cgtttaaatt tgctgaaatc aggctgacgt ttctggttga
    27601 aagcgttgcg accttcctga ccttcttccg tcatgtagaa cagcatggtg gcgttgcccg
    27661 ccagctcctg cagccccgcc tgcccgtcac agtcggcgtt cagcgcagct ttcaggcagc
    27721 gcagcgccat cgggctgttt tgcagcattt cgcggcacca acggacggtt tctttttcca
    27781 gatccgccag cggtaccacg gtgttgacaa ggcccatatc cagcgcctgt tttgcgtcgt
    27841 actgacggca caggaaccag atttctcgcg cttttttctg cccgacgata cgagccatgt
    27901 aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg gccagtctga ccgaagatgg
    27961 cattatctgc tgcgatagtc aggtcgcaca tcatgtgcag aacgtgacca ccgccgatgg
    28021 agtagccagc caccatcgcg acaaccggtt tcggacaggt acggatctga cgctggaagt
    28081 ccagcacgtt caggtgatgt acgccggaat catctttata gccgccgtaa tcaccacgca
    28141 ctttctggtc accaccggag cagaacgctt tatcgcctgc gccagtcaga atgatcacgc
    28201 cgatattgtc gtcataacgc gcatctgcca gtgcctggat catctctttt accgtcagag
    28261 gacggaaggc attgcgcacc tgcggacgat taatggtgat ttttgcgata ccgtcggtgg
    28321 atttttcata acgaatgtcc tcgaaacctt cggagcagtc gtgccattca accggtgcgt
    28381 aaggcattgc ttcatcagga taaatcatat tgtgtccttt agtcagaaac gcaagatctg
    28441 cgccagactt gcgattacgc cagcgggatt ttcccgatgc gcgttatgtc cggcgcgagg
    28501 aatgacatgg cagtcggcag ccagttccgc cgccagggcg cggaatttgc tgtcacgttc
    28561 accacataaa taataaaacg caaatgtgcg ggcgctaagg ttagcacgta aatcaggctg
    28621 gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt gcgccattat tgttgctgcg
    28681 cagcgccacc agctcccggc gttgatcgtc attgagcgag gcaaaaacag gctgttgata
    28741 ccagtcggca aataccgccg ttaacggttc tgtgcgaaaa cgctgcgccc attggcgatc
    28801 ggaacgctga cgttccgcac gttgttcagc attttgcagc cccggatgcc cgccttcgac
    28861 aataacccca caaagccccg ccagcccctg gcaagccgcc atcatcgcca cccgtccgcc
    28921 aagcgagtac cccaccagcc agaagtcaag gatgttgtaa ctaaccaagg ttttacgcag
    28981 taagtcggtg acatcatcaa atccatcgac gctaatcgcc gccgaaccac catgacctag
    29041 gagatcaaca tacaaccgtg agtagtcggc aaacgcctcg cccacttctt gccattcgtg
    29101 gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt aaacctagtt ttccgtgttt
    29161 tgcctgcgcg tgcaggatca taaatggctt acctgcgcca gaagttgctg gagcgtttgc
    29221 gcaccatcgg tgtcgttaac caccatttca atcaccgtgg tggttggcgt gcgccaggcg
    29281 tcggcaaatg ccgtttcaag ttcctgccag ttttgcggat gatgatattt cagctcgaac
    29341 atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca gatagaaacg ctcacgctcg
    29401 ctttgcggcg ttggcaacag cgagaaaatt tgcccgccgt tgttgttcac cacaattaat
    29461 accagcggcg cagaaacctg acgcaataac gccagcgcgt tgagatcgta aagcgcggag
    29521 agatcgccca caatcgccag cgtcggtttg ccgcttgccc gctgaacgcc tgcggcggtc
    29581 gaaagcagtc cgtcgatacc gctggcccct cggttgctgt acaccgggta acctgccgga
    29641 agttgcgaaa gcgcatcaat cagacgtacc accaggctgt taccaacaaa caattgcccc
    29701 tgttcaggca gatagtcgct gatgcgatgc gccagttgcg cttcgccaaa cgcatcacgg
    29761 cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg ggatttcaac gcaccagggc
    29821 tggcgttttt ctgccggatg cagctccagc cagtcggcaa tattggcaat taagcgacgt
    29881 ccgcgatggt gtgccggatc aagtcgccct tcaatgtcat caacaatcca gtactcttct
    29941 ggttcacagc ttgcctgcca ttgcaggagc cgtttccccg tcaggctact tcccagttgc
    30001 accacaattt gcgcctgctg cagctcgctg gtcgctttgg cattgcctaa ccaaagatcg
    30061 gcacacggca gcggctgccc ggtttgtgac agcacatcgc caatcagcgg ccagccaaga
    30121 gtttgcgccc acagggcaac ttttttgccc tcttccgcac tcatgcgccc ggcaaccacc
    30181 acgccgcgct tttgtcgcca gaagaaccag tcgcgctgtt tttcactttc cagacgaggc
    30241 gcttcacgca gccacggttt gtcgtcctgc caccagtcac ccagacgctg ttgccagcta
    30301 agcccggtat cgtccatttc gccatacagc ggttcagcaa acgggcagtt gatatggact
    30361 ccccccgcat gaagcgtacc gagagcgtgg tcgatggtag aaaccagcca acgtgcgggg
    30421 atatcctggg tcgggcgcgg caatgaaata ctgtgcgtgg ggtgagaggc gaacattccc
    30481 ggctggcgaa ttgcctgatt cgcgccgcag tcaattagct ccggcgggcg atcggcggtt
    30541 aagagaatca gtttttctcc ggttaacccg gcttcaatca gtgccggata gagatttgcc
    30601 accgccgtgc cggaggtcac aatcaccgcc accggctgct tgctgacttt cgccagcccc
    30661 agcgccagat gccccaaccc acgctcatcg aaatgggtgt ggtgaatgaa tgcggaattc
    30721 tccgccgccg ctaacgttaa cggtgtagaa cgcgagcctg gggcgataca gatgtgtctg
    30781 acgccgtgac gcgttaatgc ttccagaatg accgccgccc agcgtcggtt aaatgcgctt
    30841 actgacatga gtttgtccgg tatcaatatt gcggctaagt ataaggagct acaaaaatag
    30901 attattgata tgaatcggta atgatgcgac tcattactat tccatttgta ataaagtacg
    30961 cagccctgcc gctttgttgt cgatttcctg ccactcttgc tcggggtcgg aaccacggac
    31021 aatgcccgcg ccagcatata atcgcacgac attgccgcta atttttgctg agcgcaggga
    31081 aacgcagaat tcgctttgtt gtaatgagag atagcccgca gaaccggcgt accattctcg
    31141 ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga tcgcgcggta agccagcaac
    31201 tgctgccgtt ggcagcaact gatgtaaaca gatcacatca tccgctttgt tgagtgaagt
    31261 ccagatacag cggcgaagat gctgcacttt acgcagacgc agtacctgcg gcggtaaaac
    31321 atccagcgtc tgggtatcgg cctgtaatcg ttggcagata tcttccacca ccagcatgtt
    31381 ctcgcgctgg tttttatcat ccgccatcag ccactctcct aactgctgcg cctgcttatc
    31441 atcaggatga tttgctactg ttcccgccag cgcttcagta cgcagcgctt tgtcacgccg
    31501 ccgccataac cgttccggtg aagagccaag aaaagcattt tcgccatcaa aggccatgta
    31561 aaaatggtag caattcagat tcagtcgacg actggcagcc atcatcgccg ccgcgttgac
    31621 cggacttgcg aaatgcaggt cagttgcccg agcgagcacc actttgtcga gctcaccttc
    31681 ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag cccgttttgt ccggccagtg
    31741 ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc ttgatactca ccagtgtggc
    31801 gataaattct tttgcctgaa tcgcatcgtg ctgaagggag ctttcgctga atagcgtcag
    31861 ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg cggggtaaaa gtaaattgcc
    31921 ctgcgacggg tcaaaagcat tcagccccca aatgcgtaag tcagcgtgtt ccgggtgctg
    31981 gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg gtaatcgcgc ccaggacggc
    32041 agcttcttca tcaccattac gttgttgcca gtagaattgc gggtacgttt gctgactggc
    32101 cagccagctc aaggcatcaa aagcgtcttt gagagggaaa ggaatatcga taacccgaat
    32161 gccgggtgtc gccggaatct cttgcgacaa atggcgcagt agattttcca gcgccgtagt
    32221 aagtgattgc acgcggacct ctccctgtta aaagcctcac attatacggg gtactacaaa
    32281 aaaaatgcag taccccggtg tagggaggtt ttaacggcgt gccagcaaca gtcctagtac
    32341 cagcccgacg gccgcgccca caccaattcc ttgccagggt ttttcgtgga cgtagtcatc
    32401 agcacgataa acagcctgct tcgcccgata gtaataactg tctgaagcct ggctaacccg
    32461 ttttttcaca tcatccagcg ctttttctgc atgcgctttc agctcaacat atttctgatc
    32521 ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt tcactaagca gcgtcaggtc
    32581 gtcatcgata cgtgtatcac caaactgatt agacattctc gttctccatt tgcgtaaaac
    32641 ctgtcactaa ctatagacaa gagttgttga ttacgcctga attacctcgc gtgccatccc
    32701 aatgtgtggg ataccatctt cttcatagac ctccgtcaca gggataaaac cgaaactctg
    32761 gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca ggcttatcag gccagtgatg
    32821 cgtacatgtt tcgagtgttt tactcatcag ctgctgcccc actttttcac cacgcaacgc
    32881 ttcactgaca atcacccgac ctataacgac cggctcaaga tcatcatcac ttttcagaat
    32941 cctcgcatac gccaccagtt catcattttt ccaaccgaga atatggcgat tatcccccgt
    33001 caaatcatca ccatcgatat cctgataagg gcaattctgc tcgacaacaa acaccgcgca
    33061 acgcagttgt aataaggcat aaagctggga aaccgataat tcagagtgat gcagatcttg
    33121 ccattcaatc ataatgctct ccttttagat gtatgcctta tactagccgg aatatttttt
    33181 gaaacgtgat gaactcatgg aattaatttt tttaggtact tcagccggtg tgccaacccg
    33241 cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat ccgacccaga gcggactttg
    33301 gttgtttgac tgcggtgaag gcacccagca tcagctactg cataccgcct ttaaccctgg
    33361 aaaactggac aagattttta tcagtcacct tcatggcgat catctttttg gtttacccgg
    33421 cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc ttaacgattt atggtccgca
    33481 aggtatccgt gaatttgtgg aaaccgcgct gcggattagc ggctcatgga ccgattatcc
    33541 gctggaaatt gtcgaaattg gcgctggcga aattctcgat gatggcctgc gcaaagtaac
    33601 cgcttatccg ctggaacacc cactggaatg ttatggctat cgtattgaag aacatgataa
    33661 accgggtgca ttaaatgccc aggcattaaa agctgctggc gtgccgcctg gcccactgtt
    33721 tcaggaatta aaagcgggca aaacaatcac gctggaagat ggaaggcaga ttaacggcgc
    33781 agattactta gctgctccag tgccaggtaa agcgctcgct attttcggcg ataccggccc
    33841 ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc atggtgcacg aagcgacgct
    33901 ggatataacc atggaagcca aagccaatag tcgcggccat agctctacac gccaggctgc
    33961 gacactagcc cgtgaggctg gagtcggcaa gctaatcatt acccacgtca gctcgcgcta
    34021 tgatgacaaa ggttgtcaac acctgttacg tgaatgcagg tcaattttcc cggcgactga
    34081 actggcgaat gatttcaccg tgtttaacgt ttaactctgc ttattattaa aaacagggtg
    34141 aaacttgccc tgttatagca acccgcgcta catatatcca cccacacagg catattgagc
    34201 gatttttgat atttattcat ctccttacgc actatattca gagcaataag ttgcctcttt
    34261 tcatcttgat tcagcacaac ctttgcaact aaaaggagcc catgatggtt acagttgtca
    34321 gcaattattg tcaattatct caaacccaac tcagtcagac atttgcagaa aaatttactg
    34381 tgaccgagga attactgcag tctttaaaaa aaacagcgtt atccggagat gaagaaagta
    34441 ttgagttact gcataatatt gcgttaggtt atgatgaatt tggaaaaaaa gctgggtaat
    34501 gctgccaact tactgattta gtg
//
LOCUS       DAZOYF010000024        34445 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZOYF010000024 DAZOYF010000000
VERSION     DAZOYF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.024"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..332)
                     /locus_tag="ACHUOF_001139"
     CDS             complement(<1..332)
                     /locus_tag="ACHUOF_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610405.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            494..550
                     /gene="ysgD"
                     /locus_tag="ACHUOF_001140"
     CDS             494..550
                     /gene="ysgD"
                     /locus_tag="ACHUOF_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR0610406.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="ACHUOF_001141"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="ACHUOF_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR0610407.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="ACHUOF_001142"
     CDS             complement(1693..2073)
                     /locus_tag="ACHUOF_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFR0610408.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2467)
                     /gene="yigG"
                     /locus_tag="ACHUOF_001143"
     CDS             complement(2087..2467)
                     /gene="yigG"
                     /locus_tag="ACHUOF_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFR0610409.1"
                     /translation="MLRMFIPTSNGKISRHRYIFLFILINFIFAFLIIFFNDGEAGFL
                     VIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITMIAEDFACSGNGS
                     MIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="ACHUOF_001144"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="ACHUOF_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR0610410.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /gene="yigI"
                     /locus_tag="ACHUOF_001145"
     CDS             complement(3504..3971)
                     /gene="yigI"
                     /locus_tag="ACHUOF_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR0610411.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="ACHUOF_001146"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="ACHUOF_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR0610412.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="ACHUOF_001147"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="ACHUOF_001147"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR0610413.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="ACHUOF_001148"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="ACHUOF_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR0610414.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="ACHUOF_001149"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="ACHUOF_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR0610415.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="ACHUOF_001150"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="ACHUOF_001150"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR0610416.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="ACHUOF_001151"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="ACHUOF_001151"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR0610417.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="ACHUOF_001152"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="ACHUOF_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR0610418.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="ACHUOF_001153"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="ACHUOF_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR0610419.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="ACHUOF_001154"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="ACHUOF_001154"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR0610420.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="ACHUOF_001155"
     CDS             complement(14626..15441)
                     /locus_tag="ACHUOF_001155"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR0610421.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="ACHUOF_001156"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="ACHUOF_001156"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR0610422.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="ACHUOF_001157"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="ACHUOF_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR0610423.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="ACHUOF_001158"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="ACHUOF_001158"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR0610424.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="ACHUOF_001159"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="ACHUOF_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR0610425.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="ACHUOF_001160"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="ACHUOF_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR0610426.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="ACHUOF_001161"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="ACHUOF_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR0610427.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="ACHUOF_001162"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="ACHUOF_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR0610428.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22784
                     /gene="tatC"
                     /locus_tag="ACHUOF_001163"
     CDS             22008..22784
                     /gene="tatC"
                     /locus_tag="ACHUOF_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR0610429.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22826..23608
                     /gene="tatD"
                     /locus_tag="ACHUOF_001164"
     CDS             22826..23608
                     /gene="tatD"
                     /locus_tag="ACHUOF_001164"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR0610430.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="ACHUOF_001165"
     CDS             complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="ACHUOF_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR0610431.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24260..25753
                     /gene="ubiD"
                     /locus_tag="ACHUOF_001166"
     CDS             24260..25753
                     /gene="ubiD"
                     /locus_tag="ACHUOF_001166"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR0610432.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25799..26500
                     /gene="fre"
                     /locus_tag="ACHUOF_001167"
     CDS             25799..26500
                     /gene="fre"
                     /locus_tag="ACHUOF_001167"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR0610433.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="ACHUOF_001168"
     CDS             complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="ACHUOF_001168"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR0610434.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="ACHUOF_001169"
     CDS             complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="ACHUOF_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR0610435.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30244..31575
                     /gene="pepQ"
                     /locus_tag="ACHUOF_001170"
     CDS             30244..31575
                     /gene="pepQ"
                     /locus_tag="ACHUOF_001170"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR0610436.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31575..32189
                     /locus_tag="ACHUOF_001171"
     CDS             31575..32189
                     /locus_tag="ACHUOF_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR0610437.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32228..33679
                     /gene="trkH"
                     /locus_tag="ACHUOF_001172"
     CDS             32228..33679
                     /gene="trkH"
                     /locus_tag="ACHUOF_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR0610438.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33691..34236
                     /gene="hemG"
                     /locus_tag="ACHUOF_001173"
     CDS             33691..34236
                     /gene="hemG"
                     /locus_tag="ACHUOF_001173"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR0610439.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8108 a         8698 c         9302 g         8337 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggca
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaataaaaa tatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc agaccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gtcacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcatcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 atttcctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    12181 atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cttggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ctctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca
    22801 gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca
    22861 atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct
    22921 actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta
    22981 ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc
    23041 gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg
    23101 tggtctcgac tttaaccgta acttttcgac gccggaagag caggaacgcg cttttgttgc
    23161 tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca
    23221 cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca
    23281 ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg
    23341 cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt
    23401 gattccggcg gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct
    23461 cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg
    23521 tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt
    23581 caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg
    23641 cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc
    23701 ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg
    23761 ataagggggg gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac
    23821 cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt
    23881 agcgctgatg gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg
    23941 gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat
    24001 cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc
    24061 gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc
    24121 gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt
    24181 cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg
    24241 cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac
    24301 gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga
    24361 aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa
    24421 ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc
    24481 gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt
    24541 tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt
    24601 taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat
    24661 cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga
    24721 tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca
    24781 gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct
    24841 gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaacg
    24901 tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc
    24961 cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga
    25021 agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga
    25081 agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta
    25141 ctataatgaa gtcgataatt tcccggtgtt taccgtgacg catattaccc agcgtgaaga
    25201 tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt
    25261 ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt
    25321 ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta
    25381 cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta
    25441 cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat
    25501 ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc
    25561 tattgattat ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga
    25621 tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga
    25681 tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg
    25741 taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat
    25801 gacaacctta agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt
    25861 ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat
    25921 ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat
    25981 cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat
    26041 cctcaaagat catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga
    26101 tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat
    26161 tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg
    26221 tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg
    26281 tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac
    26341 cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc
    26401 cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg
    26461 ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc
    26521 tgacaggcgg gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca
    26581 tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg
    26641 gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg
    26701 ctcaaacacc gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg
    26761 aacgtctttg cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc
    26821 cagcggatga cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc
    26881 cattagcccg agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc
    26941 aaacacgccg atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg
    27001 aaccgggccg taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg
    27061 agcgcgcggc ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc
    27121 tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa
    27181 cgccggacgc agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta
    27241 attaaactgc ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt
    27301 aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc
    27361 ctgcatttca cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat
    27421 gcccgccgct ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat
    27481 cggcacatgg cctatatgct ccacgccgcc aaccagacat gcctgcgtat cgccagtcat
    27541 gatcattcgt gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt
    27601 aaccgccggg acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa
    27661 accctgctcc agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc
    27721 cagcgccggg ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg
    27781 cacgttacga aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat
    27841 gacaacctgt tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg
    27901 ctcaaccgga ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac
    27961 ttcatacagc gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc
    28021 gagggtgtcc agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac
    28081 cagcgccata tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac
    28141 ttcgttgacc atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt
    28201 cggttggctc acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt
    28261 acctttgctg tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt
    28321 ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc
    28381 tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata
    28441 tgccgggccc atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc
    28501 cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac
    28561 aaagaagccg gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc
    28621 gacaactttc gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg
    28681 cattcggtgg accgggttaa agaagtgcat cccgcagaag ttttccgggc gttccagcgc
    28741 gttggccagt tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg
    28801 tactttttgt tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc
    28861 ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga
    28921 gatcacgcca gccagtttca gaccatcgat tttgccgcgc tcaagctgct tgttcagcag
    28981 tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac
    29041 cggcacgcct ttccacgcgg actggtaagc gataccgcca cccatgatgc ctgcgcccag
    29101 cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac
    29161 atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg
    29221 gacaaaactt ttgttttcca gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc
    29281 aatggttttt actgcggtga tgggggccgg ataatgtttc cccgccgttt gcgcgaccat
    29341 tcctttagcg atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gcggttcctg
    29401 cttcggctga cgtttcgctt tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc
    29461 catcgcccct tcaatcagtt tttctgcttt gactacgcca tccaccagac cgattttcag
    29521 cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt tccagtgcac tgtcagcgcc
    29581 cagcatacgt ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc
    29641 cggcagaccg atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc
    29701 gcagccacca cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc
    29761 caggcgatta aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac
    29821 gaggaacagg gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga
    29881 acgcagcagc agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag
    29941 gctggcgacg gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc
    30001 ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt
    30061 ctcctgaatc cgcaaggtaa tctggtcgta ccagatgcat cggagtgtgt gttttgtgtt
    30121 aaaaatatgc aaataaaaga ttaaagaaat gccgatctga tcacgctcgg cagaaatcac
    30181 gctctggatg aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa
    30241 aaaatggaat cactggcctc gctctataaa aatcatatag ctaccttaca agaacggact
    30301 cgcgatgcgc tggcgcgctt caagctggat gcattactta ttcactccgg cgagctgttc
    30361 aacgtttttc tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg
    30421 gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa
    30481 ctgtggttct atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctccttc
    30541 tggactgaag atgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg
    30601 cctgctgcgc gcggcaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg
    30661 ggtattgagg ccagcaacat caacccgaaa ggggtgatcg actacctgca ttattaccgc
    30721 tccttcaaaa ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaat
    30781 ggtcatcgtg cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaatatt
    30841 gcctatctga ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg
    30901 cttaacgaac acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag
    30961 atgcgcagct tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc
    31021 cgtacctggt cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat
    31081 gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga ttaccacatc
    31141 cagttccatc agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt
    31201 gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat
    31261 ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacacac
    31321 ctcgcggcac cggcaaaata tccgtacctg cgctacaccc gtattctcca gccgggcatg
    31381 gtgttaacca tcgaaccggg tatctacttc attgaatcgc ttctggcacc gtggcgtgaa
    31441 gggcagttca gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc
    31501 attcgtatcg aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat
    31561 ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag
    31621 agatcaaaaa gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga
    31681 aagcgtttgt tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt
    31741 gggtggcggg tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg
    31801 cgggaacggc aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta
    31861 ccgctgtggt agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag
    31921 cgtatggcgg cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat
    31981 taaccgaata tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg
    32041 ggcagtgtga cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg
    32101 cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt
    32161 cattgcaatt gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc
    32221 ggcagagatg cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc
    32281 agggaccatg attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc
    32341 ttttacccag accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg
    32401 caaagagaag ggcgaactga aatcccgtga ggggtttctg atagtggtgc tgttttggac
    32461 cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat
    32521 taccgatgcg ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt
    32581 ggggctggat tcgctccctc atgccatcct cttttatcgc cagatgctgc aatggtttgg
    32641 cgggatgggg atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat
    32701 gcagctctat cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat
    32761 tgcggaaacg gcgaaaactc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct
    32821 ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat
    32881 cgctattggc ggcttctcga cacatgatgc cagtatcggt tatttcgaca gtccgactat
    32941 taacactatc attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc
    33001 actgttaagt gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat
    33061 cggcgtacag tttacgctgg tggttatttg tacactcgta ctgtggtttc ataatgtcta
    33121 cagttcggcg ctgatgacaa ttaaccaggc gtttttccag gtggtatcga tggcgacaac
    33181 cgccgggttt acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt
    33241 atgttcagct tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg
    33301 catcctgctg ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc
    33361 cgtctatagc attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg
    33421 gggatttttc tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac
    33481 gggcgtggat gatttttctg cctttgcctc agttgttgcg acattgaata acctggggcc
    33541 agggcttggc gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct
    33601 gattgccaac atgctgtttg gtcgtctcga ggtctttaca ttactggtgc tctttacccc
    33661 gactttctgg cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg
    33721 gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc
    33781 cagacggatg tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ctatgaccgt
    33841 gtggtcattg gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc
    33901 aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg
    33961 gcgcgcaaac cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg
    34021 aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt
    34081 tatcgctggt acgaccgttt tatgctcaag ctgattatga agatgtcagg cggtgaaacg
    34141 gatacgcgca aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa
    34201 atcgcccatt taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg
    34261 tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac
    34321 cactgacacg gaacaacggc aaacacgcag ccgggtcagc ggggttctcc tgagaattcc
    34381 gacagagaaa gcaaaaataa atgcttgact ttgtagcggg aaagcgtatt atgaacaccc
    34441 cgcgc
//
LOCUS       DAZOYF010000025        33535 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZOYF010000025 DAZOYF010000000
VERSION     DAZOYF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.025"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="ACHUOF_001174"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="ACHUOF_001174"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR0610440.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="ACHUOF_001175"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="ACHUOF_001175"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR0610441.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHUOF_001176"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHUOF_001176"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR0610442.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHUOF_001177"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHUOF_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR0610443.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHUOF_001178"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHUOF_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR0610444.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHUOF_001179"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHUOF_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR0610445.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHUOF_001180"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHUOF_001180"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR0610446.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="ACHUOF_001181"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="ACHUOF_001181"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR0610447.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="ACHUOF_001182"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="ACHUOF_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR0610448.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHUOF_001183"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHUOF_001183"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR0610449.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHUOF_001184"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHUOF_001184"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR0610450.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="ACHUOF_001185"
     CDS             8212..9075
                     /locus_tag="ACHUOF_001185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR0610451.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="ACHUOF_001186"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="ACHUOF_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR0610452.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="ACHUOF_001187"
     CDS             10411..11028
                     /locus_tag="ACHUOF_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR0610453.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHUOF_001188"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHUOF_001188"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR0610454.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="ACHUOF_001189"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="ACHUOF_001189"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR0610455.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHUOF_001190"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHUOF_001190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR0610456.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="ACHUOF_001191"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="ACHUOF_001191"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR0610457.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="ACHUOF_001192"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="ACHUOF_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR0610458.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="ACHUOF_001193"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="ACHUOF_001193"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR0610459.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHUOF_001194"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHUOF_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR0610460.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="ACHUOF_001195"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="ACHUOF_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR0610461.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="ACHUOF_001196"
     CDS             21859..23568
                     /locus_tag="ACHUOF_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0610462.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHUOF_001197"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHUOF_001197"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR0610463.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="ACHUOF_001198"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="ACHUOF_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="ACHUOF_001199"
     tRNA            27625..27719
                     /locus_tag="ACHUOF_001199"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="ACHUOF_001200"
     CDS             27991..29202
                     /locus_tag="ACHUOF_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0610464.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="ACHUOF_001201"
     CDS             29614..30657
                     /locus_tag="ACHUOF_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0610465.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..31600
                     /locus_tag="ACHUOF_001202"
                     /pseudo
     CDS             31172..31600
                     /locus_tag="ACHUOF_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31630..32781)
                     /locus_tag="ACHUOF_001203"
                     /pseudo
     CDS             complement(31630..32781)
                     /locus_tag="ACHUOF_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
     gene            33237..33494
                     /locus_tag="ACHUOF_001204"
     CDS             33237..33494
                     /locus_tag="ACHUOF_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610466.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
BASE COUNT         8402 a         8816 c         8263 g         8054 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
    31501 agcgacctcc attcccagag acctgcttga acctttgcca aaaggagcat tttcgattgg
    31561 tgagattggg cagtatgaca aatacaagac gacccatact gatcctaccc acgtaatgtg
    31621 gacacggccc taagcgagat tatggttttc aaattgttcc gggctgagac cgccacaggc
    31681 actgtgacga cgccagcgat tgtaatcaca ctcgatataa ttaaacaccg ttgcccgcat
    31741 tatttcccgg ctgctaaagc gttccccgtg gatacattcc accttcagcg aatgaaagaa
    31801 gctttccaca caggcattgt cataacagta gcctttcgca ctcatactgc cacgcaggtt
    31861 gtgtcgcttc agcattgcct gataatcccc tgaacagtat tgaccaccac ggtccgtatg
    31921 aacaatgacg ctttccgggc gtcttctccg ccacaacgcc atttgcagtg catcacaggc
    31981 cagttgtgct gtcattcgcg gtgacatcga ccagccaatg acggcgcgtg accacaggtc
    32041 gatgactact gcgagataca accagccctc atcggtacgc aagtacgtga tgtcacccgc
    32101 ccacttctgg tttcgggcca ctggcgctga agtcctgctc cagcagattc tccaatacgg
    32161 gcaggccatg tgcacggtag ctgaccgggc tgaacttccg gctggctttc gcccgcagcc
    32221 cctgacgacg caggctggcg gcaatggttt taatattgaa ctccggcagt tcgtcagcaa
    32281 ggcggggagc accgtatcgc tgctttgcct caaagaatgc cttatggaca gcggcatcgc
    32341 aggtgagccg aaactgttgg cgcaggctca tctggtgacg acgcctgagc cagacatacc
    32401 agccgctgcg ggcaacccga agtacacgac acatcgcttt gatgctgaac tctgcccgat
    32461 gattttcgat aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg cggccttttg
    32521 gaggatggac agttcctcag cctgctccgc cagttgtcgt ttaaggcgga cattttcagc
    32581 ggccagttcg ctttcgcgct ctgacgaact catttgttgc tgctgtttac tgcgccaggc
    32641 ataaagctga gattcataca ggctgagttc acgggctgcg gcggccacac cgatgcgttc
    32701 agcgagtttc agggcttcgt tacgaaattc aggcgtatgt tgtttacggg gcttcttgct
    32761 gattgatact ggttttgtca tgagtcacct ctggttgaga gtttactcac ttagtcctgt
    32821 gtccactatt ggtgggtaag atcaccctcc attaaaacta aaacgggcgc tttctgatat
    32881 atcatctgta agcgcagtaa taccattagt agcacctcgc gcattgatta ttgaatatta
    32941 taactggaat gttatatttc ttgttatgat gctgttcagt attataataa tgttattatc
    33001 accaaagata actcatgcaa atgagaaagt aatatgtcaa aacagtgcat cctttgtgac
    33061 caaagagttt atctgcggca ctatacttgt ctcattaata ctcagttctc tattttgcta
    33121 catatcaata tcaccatcaa ttttcatgca tacttatggc ctgagtactt ttagttactc
    33181 aattattttc tccttctcag ttttattttt cataatcgga aatcaattat caaagattga
    33241 aaaattatcc aacctatggt ttttattacc caatagccaa ttttatcgca ttacaaataa
    33301 atggttcaaa aacaggattt gccgcatcta ttacaggttt tactcaaaca ataagcgctg
    33361 gtacaatcag tttcctgtca gtaaccgtaa gcgacaacgg tgttggtccc ggaactactt
    33421 taggtcttgg ttcactaata acttcattac tgtcaatagc cgtaatcgcc attgcaggaa
    33481 ataataaaaa gtaagtgtac tctgactgcg tatgtatcta cttgaaccgc cccgg
//
LOCUS       DAZOYF010000026        33448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZOYF010000026 DAZOYF010000000
VERSION     DAZOYF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.026"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(92..1951)
                     /gene="gss"
                     /locus_tag="ACHUOF_001205"
     CDS             complement(92..1951)
                     /gene="gss"
                     /locus_tag="ACHUOF_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR0610467.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2156..3022
                     /gene="yghU"
                     /locus_tag="ACHUOF_001206"
     CDS             2156..3022
                     /gene="yghU"
                     /locus_tag="ACHUOF_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR0610468.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3051..3132)
                     /locus_tag="ACHUOF_001207"
                     /pseudo
     CDS             complement(3051..3132)
                     /locus_tag="ACHUOF_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3145..3393)
                     /gene="hybG"
                     /locus_tag="ACHUOF_001208"
     CDS             complement(3145..3393)
                     /gene="hybG"
                     /locus_tag="ACHUOF_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR0610469.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3527..3868)
                     /gene="hypA"
                     /locus_tag="ACHUOF_001209"
     CDS             complement(3527..3868)
                     /gene="hypA"
                     /locus_tag="ACHUOF_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0610470.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3861..4349)
                     /gene="hybE"
                     /locus_tag="ACHUOF_001210"
     CDS             complement(3861..4349)
                     /gene="hybE"
                     /locus_tag="ACHUOF_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR0610471.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(4342..4836)
                     /locus_tag="ACHUOF_001211"
     CDS             complement(4342..4836)
                     /locus_tag="ACHUOF_001211"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR0610472.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4836..6539)
                     /gene="hybC"
                     /locus_tag="ACHUOF_001212"
     CDS             complement(4836..6539)
                     /gene="hybC"
                     /locus_tag="ACHUOF_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR0610473.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6536..7714)
                     /gene="hybB"
                     /locus_tag="ACHUOF_001213"
     CDS             complement(6536..7714)
                     /gene="hybB"
                     /locus_tag="ACHUOF_001213"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR0610474.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7704..8690)
                     /gene="hybA"
                     /locus_tag="ACHUOF_001214"
     CDS             complement(7704..8690)
                     /gene="hybA"
                     /locus_tag="ACHUOF_001214"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR0610475.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8693..9811)
                     /gene="hybO"
                     /locus_tag="ACHUOF_001215"
     CDS             complement(8693..9811)
                     /gene="hybO"
                     /locus_tag="ACHUOF_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR0610476.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(10000..10287)
                     /gene="yghW"
                     /locus_tag="ACHUOF_001216"
     CDS             complement(10000..10287)
                     /gene="yghW"
                     /locus_tag="ACHUOF_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR0610477.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10406..11293)
                     /gene="yghX"
                     /locus_tag="ACHUOF_001217"
     CDS             complement(10406..11293)
                     /gene="yghX"
                     /locus_tag="ACHUOF_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR0610478.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11450..12490
                     /gene="gpr"
                     /locus_tag="ACHUOF_001218"
     CDS             11450..12490
                     /gene="gpr"
                     /locus_tag="ACHUOF_001218"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR0610479.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12530..13024)
                     /locus_tag="ACHUOF_001219"
     CDS             complement(12530..13024)
                     /locus_tag="ACHUOF_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR0610480.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13215..14099
                     /gene="yghA"
                     /locus_tag="ACHUOF_001220"
     CDS             13215..14099
                     /gene="yghA"
                     /locus_tag="ACHUOF_001220"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0610481.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14371..14796)
                     /gene="exbD"
                     /locus_tag="ACHUOF_001221"
     CDS             complement(14371..14796)
                     /gene="exbD"
                     /locus_tag="ACHUOF_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR0610482.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14803..15537)
                     /gene="exbB"
                     /locus_tag="ACHUOF_001222"
     CDS             complement(14803..15537)
                     /gene="exbB"
                     /locus_tag="ACHUOF_001222"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0610483.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15789..16976
                     /gene="metC"
                     /locus_tag="ACHUOF_001223"
     CDS             15789..16976
                     /gene="metC"
                     /locus_tag="ACHUOF_001223"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR0610484.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            17116..17775
                     /gene="yghB"
                     /locus_tag="ACHUOF_001224"
     CDS             17116..17775
                     /gene="yghB"
                     /locus_tag="ACHUOF_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR0610485.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17815..18714)
                     /gene="yqhC"
                     /locus_tag="ACHUOF_001225"
     CDS             complement(17815..18714)
                     /gene="yqhC"
                     /locus_tag="ACHUOF_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR0610486.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18908..20071
                     /gene="yqhD"
                     /locus_tag="ACHUOF_001226"
     CDS             18908..20071
                     /gene="yqhD"
                     /locus_tag="ACHUOF_001226"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR0610487.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20176..21003
                     /gene="dkgA"
                     /locus_tag="ACHUOF_001227"
     CDS             20176..21003
                     /gene="dkgA"
                     /locus_tag="ACHUOF_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR0610488.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            21203..22129
                     /locus_tag="ACHUOF_001228"
     CDS             21203..22129
                     /locus_tag="ACHUOF_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR0610489.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22180..22437
                     /gene="yqhH"
                     /locus_tag="ACHUOF_001229"
     CDS             22180..22437
                     /gene="yqhH"
                     /locus_tag="ACHUOF_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR0610490.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22480..24699)
                     /locus_tag="ACHUOF_001230"
     CDS             complement(22480..24699)
                     /locus_tag="ACHUOF_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR0610491.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24810..26222)
                     /gene="ftsP"
                     /locus_tag="ACHUOF_001231"
     CDS             complement(24810..26222)
                     /gene="ftsP"
                     /locus_tag="ACHUOF_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR0610492.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26297..27034)
                     /gene="plsC"
                     /locus_tag="ACHUOF_001232"
                     /gene_synonym="parF"
     CDS             complement(26297..27034)
                     /gene="plsC"
                     /locus_tag="ACHUOF_001232"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR0610493.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27268..29526)
                     /gene="parC"
                     /locus_tag="ACHUOF_001233"
     CDS             complement(27268..29526)
                     /gene="parC"
                     /locus_tag="ACHUOF_001233"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR0610494.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(30072..30554)
                     /locus_tag="ACHUOF_001234"
     CDS             complement(30072..30554)
                     /locus_tag="ACHUOF_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR0610495.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30607..30999)
                     /gene="ygiW"
                     /locus_tag="ACHUOF_001235"
     CDS             complement(30607..30999)
                     /gene="ygiW"
                     /locus_tag="ACHUOF_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR0610496.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            31151..31810
                     /gene="qseB"
                     /locus_tag="ACHUOF_001236"
     CDS             31151..31810
                     /gene="qseB"
                     /locus_tag="ACHUOF_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR0610497.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31807..33081
                     /gene="qseC"
                     /locus_tag="ACHUOF_001237"
     CDS             31807..33081
                     /gene="qseC"
                     /locus_tag="ACHUOF_001237"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR0610498.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(33127..>33448)
                     /locus_tag="ACHUOF_001238"
     CDS             complement(33127..>33448)
                     /locus_tag="ACHUOF_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFR0610499.1"
                     /translation="KIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICSIPSV
                     SGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLFWGRF
                     IFCY"
BASE COUNT         8250 a         8928 c         8416 g         7854 t
ORIGIN      
        1 ataaatattg gaatatataa atattgaata ttttgattaa tccccgtact aattattctt
       61 cataatcagt acggggttgc tacaacatgt attacttttt caccacaatt aacggttcaa
      121 tatcactctc ttttttgatg accagtgatt catcaccgcg caaacacgtc ccaccgtagt
      181 tgccgccaac ggtgaaggta catacctgaa tgtatttacc gtccactttc ggcaaacacc
      241 acagttgctg atagatgttt ttctgctcgg caaatttacc gctggttttg tccagcacct
      301 cttcatgatg gctgaccagg tcgatattgc taccacagcg accggcgatc ggtttcactg
      361 cgtaacctgt tttcaccaat tcatcattaa cagtgaaatc ggtatccagc aggtaacgat
      421 ggtgcgggaa cagcgaccag aggatcggca gaatcgcttt gttgccgggg atcaccgtcc
      481 acagcggctc aaagaccagc acttccgggc gcagcaatac gtcgataaga cgcacttcgt
      541 tttgcggatg accggtacgg attggcaccg cagcaaactc acggtcgcta acttcacgaa
      601 tctgatcaaa cgcggtttcc cacgcccagg ttttccacac gcagttaacc agtcgccctt
      661 ccccatcaat cagttgcccg gcagcatccc agcccagttc atccagcccg cgcaagatac
      721 gcgtttcaaa gcccgcctgg tgcagcgcct gctccataaa ctgcgcgtga tagttttcct
      781 cgatatcttt gtcctgcatg atatggacaa acggacgtgc acgactgtgt ttccaggcac
      841 cagccagttc gttaatcagc ccttccgccg gattgaagcc gttgccttta tagccctgct
      901 ccgcccaacg ttcgaggatc aagcccgctt cggtatgaca ggaggcggag tcggcgttgt
      961 attcgtaaac cttcaggcca cgctcatcca tgcagaaatc catacgacca gtgatcatat
     1021 ggtgacggcg acgctgccag gagagacgca aacgtggcca gaggattttc ggaatgtcga
     1081 acagcgccag caggttgtca tctttcagca ccttgtcggt tgcgtgaaga tacatcagat
     1141 gcagctcgtt ggtggcttta attagctcct gctcggcact ctcggtaatg gtgtagtaat
     1201 gatacggatc ctgattgatc acctgaccgt tggcctgcac ataggcgttt tgtagcggat
     1261 ctttttcatc cagccattta ccgtcaaact ggcctttgtt ttccagacgc gctccactga
     1321 ttttcagcag ctcgcctgca atttccggct gcggtaagct gtattcagta tcttccgtct
     1381 ggatcatcca gcccagaatg gtggtgtcat caaaagtgtc tttaagggta tagcagccgt
     1441 tttcgaccac catctccagc tcgcgcgtcc actgttgccc ttgcggcaac ggggaatgaa
     1501 tcacgttctg ttccgcaata cggactttgt tgccatgcaa ttgggtaatg atggcgacat
     1561 ggccagtgtc tttaaattca ccgcctttat cccagataag aagcgcaccc gcgaccggcg
     1621 cacgcggcga gccgttagga aatgcctgca atggcaggat gttgtcatta accacttctc
     1681 gcaggaagcg cagcgagaaa atctcccacg ccatacccac gtcagtaaag accacaccgt
     1741 aattcagaaa gagaaaacgg cgagcaaatt caacgcattg ccacttgtgg cccatatatt
     1801 cgtcgtcgat atagctacgg aatacggcgt catcttcgta ttcctgcgga tcgagagaac
     1861 tgtaatctga agagtagatt gctaccccac ctggggcgta gcccaataat gtcccgaacg
     1921 gggcatcctg gctggtcgtt cctttgctca tcactttacc tttaacaata caacctaagc
     1981 agttggcgta attgttgtgc tctgattacc tgctcagcga cattaaccgg acagaggtca
     2041 gactaatcat acacctcatc gcagcggctg gcgcaaaggg ttaaaaaatt cacattctgt
     2101 acagcaagtg acctgctaca ctgcttcaac actaccactc agaaggcaac tcactatgac
     2161 agacaatact tatcagcccg cgaaagtctg gacgtgggat aaatccgctg gcggcgcgtt
     2221 cgccaatatc aatcgcccgt tttctggtcc gacgcatgaa aaaacgctgc ccgttggcaa
     2281 acatccattg caactttatt cgctgggaac gccgaacggt cagaaagtaa cgattatgct
     2341 tgaggagcta ctggcgctgg gcgttactgg tgcagagtac gacgcctggc tgattcgtat
     2401 tggcgatggc gatcaattct ccagcggctt tgtcgaagtg aacccaaact cgaagatccc
     2461 ggcgctgcgc gatcatacgc ataatccgcc gatccgcgtg tttgaatctg gttcgatcct
     2521 gctttatctg gcggagaaat ttggctactt cctgccgcag gatctggcaa aacggactga
     2581 aacgttgaac tggctgttct ggttacaggg tgccgcgccg ttcctcggcg gtggttttgg
     2641 tcacttttac cattacgcgc cggtgaaaat tgagtacgcc atcaaccgct ttaccatgga
     2701 agccaagcgc ctgctcgacg tgctggataa gcaactggcg cagcataagt ttgttgcggg
     2761 cgatgagtac accattgcgg atatggcgat ttggccgtgg tttggcaacg tggtgctcgg
     2821 cggcgtgtat gatgccgcag agtttctgga tgcgggaagt tataaacatg tgcaacgctg
     2881 ggcgaaagaa gtgggcgaac gtccggcagt gaaacgtggg cgtattgtta accgcaccaa
     2941 cgggccgctg aacgagcagt tgcatgagcg ccatgacgcc agtgattttg agacaaatac
     3001 agaagataag cgtcaggggt aagggttggt gttcgtcgca gcaagccatc caggccggat
     3061 aaggcgttca cgccgcatcc ggcaatcgtg cacaatgcct gatgcgacgc tgccgcgtct
     3121 tatcaggcct acaaaagcta atcatcacgc gctggtaatg tcgtaatcca tttggcgcag
     3181 tgcgtctaat gtggctttgg cttcatcttc gtcgatgatg ctcatggcaa atccgacgtg
     3241 taccagcacc cactggccca gcagatcggc agggttacct tcgcaaatca gggcgatatt
     3301 cacgtcgcgc ttgataccac atacttcaac ctgcgcaagc tggtgaatat cttcaccgac
     3361 agccagcacc tggcctggaa cgccaataca cattgttaac tccggttatt tcgattgccg
     3421 gacaaacgcg aaaaagaacc tttagcgggt gttccctctc accctaaccc tctccccgga
     3481 ggggcgaggg gaccgtccgt gcgcttttta ggcgttgtcc tcagtcttat tcaacttcaa
     3541 tacttttgac gatcagcgaa tcgccggtat cgacacgcaa ccgctcgccg tgacagagcg
     3601 gacactgcgc atcgtgctga tgaatctcca ccacctggct gcaatcccag caccaggcct
     3661 gggcgggttt atagacgata tgtaaatcgc acccttgcgc caccgttccg tggcagacaa
     3721 tttcaaaact aaaacggacg gcgctctcct caacgcagga gagcgcgcca atttccagcc
     3781 acacggcggt gacgcgctta acatcgtgct gctccgcctg ccgttggata atttcaacgg
     3841 cgctctggca aagcgacaac tcatgcattt tcgcccctcc tgcggccaaa cagcagggcg
     3901 cgacgccctg cgtgtggtac atccggattg gtgactggca acgaaaggat cattcgtgcg
     3961 cagtcatccg tcaggcgttg gccttcttca atcgacatgc tgtgcgaaag cggcgacatc
     4021 agcgaacagg agaggtattg cgaaacaccg tccagttcgc caacggtaaa ggtcatggtg
     4081 ccatacggca gttgcagacc aattttttca ctcactttgc gcagcggcca gagttgatcc
     4141 gggccgggga agataactgc actcagcatc cacggggtga tcacacaccc cgtccactga
     4201 ccttcgaaca gcgtaaaatc agaaacatac accggcattg aaggatgcag aaaagagaga
     4261 tcgtgcatcg aacgccgggc aatttcttca aacgctgcct gtacttgcgc cttcggggag
     4321 gtctggaaac ctgctatctc ttcagtcatg aatcgcctcc cgtgggatgg cttccacgcc
     4381 tgattcgcgc agcgcagcca gaacctgctc aagcgcaggt tcaatcattg cttcaaccgt
     4441 cggcgttaag ccgatgtgtg gctccagcga ttccgggatc acgccgacca gggtcagctt
     4501 tttcggaaac tcaccggtga agcgcagggc cgacaatacg tcggccaggc caagctgatg
     4561 cggagagatt ttgttggtaa acaacgccgg aacttcttca tcccgcagga tcatcatcgt
     4621 tcccggagtg ttctttttcg acacaatggc atccgcaata atcagatgat cgcgatttgc
     4681 catgtcgcca agcagctcca ttcccgccgt gccgccatcg aggatctcaa cataatccgg
     4741 cagaatgtat cgttgctcta acgcttcgac aatccgcacg ccgatggctt catcggtcag
     4801 caaaatattg ccgaccccta agactaaaat acgcattaca gaaccttcac tgaaaccact
     4861 tcgttgccgt cggcatccac tacgtgtacc gcacaggcca tgcacgggtc gaaggagtga
     4921 atggtacgca ccacttccag cggtttattc ggatcggcaa ccggtgtacc caccagcgac
     4981 tgctcgtaag gaccgacgtc atcattgaag ttacgcggac cagagttcca ggttgatgga
     5041 acaaccgcct ggtagttgct gatgataccg tctttaataa ccatccagtg agagagcata
     5101 ccgcgcggcg cttcgaggaa gccaacacct ttgaactcac ccgttgccgg aatgttcggc
     5161 ttcacaaagg tggtgtgatc gcctttgccg atattggtga tcagtgcact gtattggttt
     5221 tgcaggatat cctgcaattc acagcagtga acggtacgac caataatacg gcccagcgtg
     5281 gagtgcagct gtgccacttc cagcgtgttg ccagtcagtt tctgataaat cgcaacgatt
     5341 tcattcagtt tgttctgggt agattcgcga cctgccgcca gtttcaccag catattagcc
     5401 agcggaccca cttctaccgt tttgccgtag aaagtcggtg atttcaccca ggaatatttc
     5461 ccgtcgtcag accaaccatc ataagccgga atggtggtac cttcccacgg cgcctgtggc
     5521 gcttcgtctt tataccagga gtgcttcgcg ctttcctgaa tgcctttgat caggtattca
     5581 tcggaatgag aagtgatcgg acgatacgag gacagatccg cattctcaat gtagcctccc
     5641 gggaacagga agctgccgtt tttgctgtcg gtcgggaatt ccggcacgct caggtagttc
     5701 accgcacctt taccgcgcgt cagccattcc gggtagaacg cggcgataac cgcggtatca
     5761 accttataaa cctgctcaac aaagtcgctc agtttgtcga tgaaagactt gatgtacatc
     5821 aggcgctcaa ggttcaacac gcccaacccg tcgaggttga ttgggttcgc gacaccaccc
     5881 acagccaggt tctgaatgtg cggcgtttta ccgcccagca gcgccacgac gcggttagcg
     5941 tcacgctggc actccagcgc ctgcaggtag tgcgctaccg caatcaggtt cacttccggc
     6001 ggcagtttca tcgccgggtg accccagtag ccattggcga aaatacccaa ctgaccgctg
     6061 gcaaccagat ctttgatctt gttctgaact ttagtgaact cttccggact gttcagatgc
     6121 caggtcgaaa cgcctttcag catttcggag gctttggttg ggtcagcttg cagtgcagaa
     6181 gtgatgtcca cccagtccag cgccgaaagc tgatagaaat gaacgatatg gtcatgcgtg
     6241 gtgtgcgcag ccagaatgat gttacggatg tattgcgcgt taaccggaac gtcgatattc
     6301 agcgcacttt ctgccgcacg aacggaagac agcgcgtgag tggtagtaca tacgccacag
     6361 atacgttgca caatcatcca tgcatcgcgc ggatcgcggt ttttcacgat ctcttccatg
     6421 ccgcgccaca tggtaccgga agcccatgct ttcgaaacga cgccattttc gatttcgcaa
     6481 tcgatgcgta aatgcccctc aatacgggtt accggatcaa tagtaattct ctggctcatg
     6541 ctttgctcgc ctcatgacga ttatgatcgt tttgttttaa aggaggaagt atcggcagta
     6601 gacgaatgag tacgatgtaa gcgcaaatct caatagccac aaaaccaata gaaatcaaca
     6661 gttcttccca ggtcgggaag taggcgtaac cgccgcccgg gttgaatgcc accaacgaat
     6721 aggtcagacg ccaggttgca caacctaaca gtgcgctcag tgctgacagg aacagcatac
     6781 gggaatcatt acgcagcttc gccacacgca gaacgaccag cgggaagagc atcagcagga
     6841 cttcaatcca gaacatcacg gagtagaagt caccggcaaa cgctaacgac agcttgtcgc
     6901 gatagataag ctcgccaaag cgcagcccga tgaaaatcgc cagcaacaca ctgatggtgt
     6961 tggtcagctt gacgaacagg ctcttttcat ccggaccgtt gccacgcaga cccgcctgca
     7021 ccagtgaacc ttcaaagatg acaatcgaga agcccatgat gaacgccgtc agcagcgaga
     7081 acagcggcaa catttcatag ctctgccaga gcggatgcac cttgtagccc gccgagatca
     7141 tcagcgaccc cattgaagac tggtgcatgg tcggcagcag cgcaccgagc gcgatgatga
     7201 agaacatcac cttgttcaga cgttgtagcg acaccttcca gcccagacgt tcaaacagtg
     7261 ccggagcaaa ctccagagcc atcacgccga tgtagatggt catacagacc gccgtctcga
     7321 acagtaccga gttcacgttg aagtgacccg gaatgtagaa gtacggcagg ttccagtagc
     7381 gacccacgtc gatagtgatc gacaagccac ccagtgagta accaaacaga ctcgccaaca
     7441 gcgccggacg caccagcgga tggtattgcc cacggttaaa gacgtatacc gcccacgcca
     7501 gcgcccagcc gccacaggca aagccggtgc caatcagcag gtcaaacgcg atccacacgc
     7561 cccacgggaa gccgccgttc aggtcagaga cagagcccag accgaacacc agacgcttca
     7621 caatcaggag catacagatg acgattaacg gtccaaaaat catgaccggt ttactgatga
     7681 ttttgccgcc cagcggttgt ggatcatgac tcatgatcgt ctcctccgtc gtgatggtcg
     7741 tttttggtgt tgcgacgaac cagcacggtt aagcccgcca gcacagccag tggtagcatc
     7801 atgcctttat acagggtgtg ttgaatattt tcggaacgcg caccggtaga aagatcgtcc
     7861 agtttcggca ggtcgagatt ttcataaggc acacccgtca gtaccagaac ctgagtaccg
     7921 ccgccctctt tctcgccgta cagatgcgga taatatttcg gcaccgtatg caggtaagtg
     7981 tcgccagatt tcagcgtctg acgcggatag tggtattcgc tgccaggctt cagcgccaga
     8041 cgttttttcg cctccgccat cagctcttca cgcgtaccga aaatcaccgc gcccgccggg
     8101 cacacttcta cgcagccagg cagaccgcct ttatcgagac gttccacacc tttctggttg
     8161 cacagctcgc acttatgcag cgcaccaaac gggttgttgt agtcgtactt cggcacgttg
     8221 tacggacagg cgaccatgca gtaacggcaa ccggtgcaca cgtctttgtc gtaatggaca
     8281 atgccggttt tcggatcttt tttcagtgca gagaccgggc acacagagac acagttcgga
     8341 tcgacgcagt gcatacactg tttcttaatg tacgcgtagc cgttctcctc ctggtctttg
     8401 ttgacccctg tgccgctggt ccacacctga atgatgttat tggtatacgg cgacagtttg
     8461 tcgttgttcg accaggtctg ttccccttgc gggttacgtt cagggaagtt gatatcctga
     8521 cacttggtga cgcaagcctg gcagcctacg cacaaggtcg agtcgtacaa catccccagc
     8581 gatcccggaa ttggcgggcg gttttcagca gccgcatgac tgacagacgg cagcgcgccc
     8641 gtcagcaatg ccccgcagga ggctgcttta ataaaattac gtctgttcac ggttattctc
     8701 cccgtgagtc agcgttatct ttcttttgct gacgacccag ttcacgcacc gccatcacgc
     8761 tgacaccggc aaccagccca accacaccgc cgagcaaacc aatagcgcct gcagagacgt
     8821 tgccgccctc tttagcgtta acatccggtt tctgtgaacg cggagtctga ttttcgacgt
     8881 tggcaagctg atggatgcct ttatggaagc cgataccttt ttcgttacag ccatagcacg
     8941 ggtgaccaat cgccaccggc cacacaccgc caacatcgca gaattgcagc gttgagcagt
     9001 tgccgtaagt ttctggccct ttacagccga ggtggtacaa gcaccagcct tcgcggtggc
     9061 cttcatcacc gaactctttg gcaaaacgac cagcatcgaa gtgcgggcga cgttcgcagt
     9121 gttcgtgaat cagacggcca taggcgaagg tcggacggtt tttgtcatcc agtttcggcg
     9181 gtttgccgta agtgatgatg tgcgcaacgg tcgcgaggaa gttgtgcggg ttcggcgggc
     9241 agcccggaat attgataacg gttttgcctg gcagaacttc ttgcaagctg actgcgccag
     9301 ttgggttaac tccagctgcg gcaacaccgc cccacgcgga gcaggaacca atagcgataa
     9361 tggctgctga gccttccgcc gctttgcgga tgtgatccac aatcggctca ccggcaacca
     9421 tgcaataaat accgttatct tttaatggga tggaaccatc caccactaac acatactgcc
     9481 ctttgtactt ctcgagagcg ttatgtttgt tctcttcgac ctgatgaccg aaggcggcgg
     9541 aaagcacttc gtgatattcc agagagatag tctccagcac gaggttttct accgttggat
     9601 gcgttgcacg aagcagagat tccgtacaac cggtgcactc ctgcgcgcca atccagataa
     9661 ctggcggacg ctgcgggtta gtaaccgatt cggccatctc tgcagcggct ttgctactta
     9721 accccatggt ggcggctaat gctgcacaaa gcttcatgaa atcacgacgg ttaatgccgt
     9781 gagaatggat gagggtgtta tctccagtca tttatagtta ttccgttgcg agaacctggc
     9841 atatattttg cctcaatcgc aaaatcaata atgcgatcga tgcgccattt accacacatt
     9901 tattatggtt atcgatatca tgatactgcg gcggggcgct aaaacgaagg gaagaagcat
     9961 tagtgtaatt aattaaagca agataatacg tatgtttgat caattttcga ggcgataacc
    10021 ggccataaaa aagcgcaacg tagaacagga attattctca cgaaagaaat ccattaccat
    10081 ctctttatcc agtccatagc ggcgcgtcag aatacccgct tcccaggcgc taagctggcg
    10141 atctccggtc ttttcgtaca tcctgtgtga gtagcctaat acaaaaccgc gtttataatc
    10201 ggcacagaat tttgtcacat taaccgcact gtcggcatgc gttgctttta atcccgccat
    10261 taagccttta ccaaaatggt tattcatcgt tcacctcatt cattatatat cgccatatat
    10321 aacgatattt taggcaaaga acgacttttg cgcacgctat tttttcatac ttgtttaaga
    10381 cgtaatcccg taagagataa acctatcagg agagatattt atcgaaccat ttcagtgtcc
    10441 tttgccaggc aagatcggcg gcagatttgt cataacgggg cgtggaatca ttatggaatc
    10501 cgtgattaac ccccggatag atataagcct cataaacctt attattgtct ttcaacgccg
    10561 cctcgtaagg aggccagccc tcgttgattc gggtgtccag ttccgcgaag tggagtagta
    10621 aaggcgcttc aatcttcgcc acatcggcag tgggtgcctg acgaccataa aacggcaccg
    10681 cgcaggccag ttccggatac gcgacagccg ccgcgttcga tacgccaccg ccatagcaaa
    10741 atccggtaat acccacttta cctgtcgctt gcggatagcg ttgcataaac tcaattgcgg
    10801 caaagaaatc attcatcagt ttggttggat caacctgctg ttgcagctca cgacctttat
    10861 catcatttcc cggataacct ccaacggaac ttaatccgtc aggtgccagg gcgatatacc
    10921 ccgccttcgc cactcgccgt gccacatctt cgatatacgg attcagtcca cgattctcat
    10981 gcaccacccc cacggctggc gttttgccgc tcatttttgc gggtttcacc agataacccc
    11041 gtacctcgcc gtgaccattt ggcgaaggat acgtgatgta ctcagcaaca atctccgggt
    11101 cggtaaactc tacctgagtc gccagcgcat aatttggctt gagcaaatca aacaacgcta
    11161 atgccgtcat cccgcccacc gcatacttcg ccgcaaggtt gaggaactca cgtttcgaaa
    11221 ttttcccgtg aacgtaatag tcgtagtaat ccaacaactc ttgtgggaaa tctttggcgg
    11281 ttaaacgcgg catcgttgca ttcctcaact ggtgtttttt taagcaaagc ataagcgcgt
    11341 gtttttgccc agtttttcgt cactctgtga gccagactac gggatacgcg ctggcgaatc
    11401 gctaaactag aaacattgtt tcgaaattga acggtggaaa ggagaggtca tggtctggtt
    11461 agcgaatccc gaacgttacg ggcagatgca gtaccgctat tgcggaaaaa gtggtttacg
    11521 cctgcccgca ttatcgctcg gtttatggca caatttcggt cacgttaacg cgctggaatc
    11581 acagcgtgca atcctgcgta aagcgtttga tttaggcatt acgcactttg atttagccaa
    11641 caattacggg ccgcctccag gaagcgcaga agagaacttt ggtcgcctgc tgcgcgagga
    11701 ttttgctgct tatcgcgatg aactgattat ctctaccaag gctggctacg atatgtggcc
    11761 cggcccgtac ggctctggcg gttcacgtaa atacctgctc gccagcctcg accaaagcct
    11821 gaagcgtatg gggcttgagt atgtcgatat cttttactct catcgcgtcg atgaaaatac
    11881 gccgatggaa gaaaccgcct ctgcgctggc tcatgcggta caaagcggta aggcgctgta
    11941 tgtcgggatc tcctcttact cgccagagcg gacgcaaaaa atggtcgagt tgctgcacga
    12001 gtggaaaatt ccgcttttaa ttcatcaacc ttcgtacaat ttactgaacc gctgggtgga
    12061 taaaagcggc ctgctggata ccctgcaaaa taacggcgtg ggctgtattg cctttactcc
    12121 tctggctcag ggattgctga ccggaaaata tctcaacggc attccgcaag attcacggat
    12181 gcatcgtgaa gggaataaag ttcgtggtct gacgccgaaa atgcttaccg aagccaacct
    12241 caacagcctg cgcttattga atgaaatggc acagcagcgt ggacaatcaa tggcgcaaat
    12301 ggcgttaagc tggttgctga aagatgatcg cgtgacgtcg gtattgattg gtgccagccg
    12361 cgcggagcaa ctagaggaga acgtgcaggc gctgaataat ctgacattta gcaccgagga
    12421 gctggcgcag attgatcagc atatcgccga tggcgagctg aatctgtggc aggcgtcgtc
    12481 cgataaatga cctgttaata acgggcgcgc gaaccgcgcc cgaatcagat cagtgattat
    12541 gacgagtcag tcggtcaaga tagcccatca caaatgcaga aagcacaaac gtcagatgga
    12601 taatgacgta ccacatcagt ttgttatccg ggacattttt cgcatccata aagacgcgca
    12661 gtaagtgaat ggaagaaatt gccacaatcg acgctgctac tttgtttttc agcgacgttg
    12721 cgtccatttt ccccagccac ttcagcttct ctttgttctc ggagatatcc agctgtgaga
    12781 cgaaattctc ataaccggaa aacatcacca tcaccagcaa accgccaacc agcgtcatat
    12841 ccaccagcga cagcaacacg aggatcaaat ctgattccgc catcgagaag atattcggca
    12901 gtacgtgaat aatctcctgg aagaacttca gcgccagggc aactaacgcc agcgaaaggc
    12961 caaagtacac gggggcaagc agccagcgag aagcatacat tgcattttca agaaaacgtt
    13021 ccatacattc ctgtcgtgta gttaaaacgg gcaacagtat atcgcaattg agtaaacagc
    13081 acgcaacagc aaatcaacat atacgttaaa cctttgtcgc ataattctga ggtttaggga
    13141 tcttcctggt tcccttatca acgcatcgag agcgtctacc cttaaaggac gttgaacctg
    13201 aagggagaaa aacgatgtct catttaaaag acccgaccac gcagtattac actggtgaat
    13261 atcccaaaca gaaacaaccg acgccaggca tccaggcgaa gatgacaccg ataccggatt
    13321 gcggcgagaa aacctatgtt ggtagcggtc gcctgaaaga tcgtaaagca ctggtgacag
    13381 ggggcgattc cggaataggt cgcgctgccg ccatcgctta cgcgcgtgaa ggggctgacg
    13441 tggcgatcag ttatcttccc gtggaagaag aagacgctca ggatgtgaaa aagatcattg
    13501 aagaatgcgg acgcaaagcc gttctgctgc caggcgattt aagcgatgag aaattcgccc
    13561 gttcgctggt tcacgaagcg cacaaggcgt taggcgggct ggatattatg gcgctggtcg
    13621 ccgggaaaca ggttgccatt ccggatattg cagacctcac cagcgaacag tttcaaaaga
    13681 cctttgccat taacgttttc gcgctgttct ggctaaccca ggaagcgatc cccctgctac
    13741 cgaaaggtgc aagtgtcatc accacttcgt caatccaggc ataccagcca agtccgcatt
    13801 tactggacta tgcggctacg aaggcggcga ttctgaacta cagccgtggc ctggcaaaac
    13861 aggtcgcgga gaaaggtatt cgggtgaata ttgtcgcgcc aggccctatc tggacggcac
    13921 tgcaaatttc cggcggacaa acgcaggata agatcccgca gtttggtcag aaaacgccga
    13981 tgaagcgtgc ggggcaaccg gcggaactgg cccctgtata tgtttatctg gcaagtcagg
    14041 agtcgagcta cgtcaccgca gaagtgcacg gcgtgtgcgg cggcgagcat ttaggttaaa
    14101 aaaatgcccg gttgtgaaaa gcaaccgggc atcattgtga attcactctt acctgatgtg
    14161 gcgaaaacgc cttatccgcc tacaaacgca tgaagattca acatgttgca atagattttg
    14221 taggcctgat aagcgtagcg catcaggcat tgagcaccta atgccggatg cgtcgtgaac
    14281 gccttatccg gcctacaaac gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct
    14341 gatgagcgta gcgcatcagg caattctact ttacttcgct ttggcggttt cttcgccgac
    14401 cagacctatc ttcaggtaac ccgcctgatg cagcgtatcc attaccttca tcaacgtctc
    14461 gtaatcgacg gttttgtccg ctcggaagaa gatggtggtg tctttcttgc cttcggttaa
    14521 cgcattcaac gccgtaatca ttgtttcatc ggtgaccgga tcgttaccga taaacatcga
    14581 gttgtctgcc ttcaccgaca gataaaccgg tttttccggg cgcggctgcg gcgtgctggt
    14641 agaagcaggc aagttcacct tcacatctac cgtcgctaac ggtgccgcca ccataaagat
    14701 aatcagcaga accaacatca cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc
    14761 gttatcgtcg aggttttcgt taagatgcat tgccatcgga cattatcctg cgcgtaattt
    14821 ttgtgcgaca cgaaccggat gcgcagcggc gctggcttcc agatctaggt cacggctttg
    14881 cagcaacaat acctgcgctg caacatcacc cagcatcgct ttaaagccgc caatctggcg
    14941 tgcaaatacg ttatagataa cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc
    15001 taacagagct tctgcgatac ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc
    15061 gataccaata aagctgttca tgatgcccca gaccgtacca aacagaccaa cgaacggaga
    15121 aatcgcgccg atggttgcca gatagccgtt accgcggccc atttgacgac ccactgcggc
    15181 gacccgacgc tccagacgga agctggtacg ttctttaata ccttcgttat cgtcgctgcc
    15241 ttctgacagt tccagctcat tctgcgcttc attgagcaaa tgcaggctta agcttttgct
    15301 accaaaatca gcggcgatat cgttggcctg gtttaaggaa cgtgcttcag ccagcagttg
    15361 ctgctcgcgc ttaaggcgac gcttctgatt gaagaactct acgctcttac tgaagaagat
    15421 tgcccaggtg actacagagg ccaaaataag cccaatcatc acgcacttaa cgacaatatc
    15481 ggcgtactga tacatacccc agacggaaag gtccgtctgc attaaattat tacccactgt
    15541 gtatctccag gacgcaagtc acaaaatctg cgcataataa tatcaaaacg acgtcgaatt
    15601 gatagtcgtt ctcattacta tttgcatact gccgtacctt tgctttcttt tccttgcgtt
    15661 tacgcagtaa aaaagtcacc agcacgccat ttgcgaaaat tttctgcttt atgccaattc
    15721 ttcaggatgc gcccgcgaat attcatgcta gtttagacat ccagacgtat aaaaacagga
    15781 atcccgacat ggcggacaaa aagcttgata ctcaactggt gaatgcagga cgcagcaaaa
    15841 aatacactct tggcgcggta aatagcgtga ttcagcgcgc ttcttcgctg gtatttgaga
    15901 gtatggaagc caaaaagcac gcgacgcgca atcgcgccaa tggcgagttg ttctatggac
    15961 ggcgcggaac gttaacccat ttttccttac aacaagcgat gtgtgaactg gaaggtggcg
    16021 caggctgcgc gctatttccc tgcggggcgg cagcggttgc taattcgatt cttgcttttg
    16081 tcgaacaggg cgatcatgtg ttgatgacca acaccgccta tgaaccgagt caggatttct
    16141 gtagcaaaat cctcagcaaa ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg
    16201 ccgatatcgt taagcatctg cagccaaaca ctaaaatcgt gtttctggaa tcgccgggtt
    16261 ccatcactat ggaagtccac gacgttccgg cgattgttgc cgccgtgcgc agtgtggtgc
    16321 cggatgccat cattatgatc gacaacacct gggcagccgg tgtgctgttt aaggcgctgg
    16381 attttggcat cgatgtttct attcaggccg ccaccaaata tctggttggg cattcagatg
    16441 cgatgattgg cactgccgtg tgcaatgccc gttgctggga gcagctacgg gaaaatgcct
    16501 atctgatggg ccagatggtc gatgccgata ccgcctatat aaccagccgt ggcctgcgca
    16561 cattaggtgt gcgtttgcgt caacatcatg aaagcagtct gaaagtggcc gaatggctgg
    16621 cagaacatcc gcaagttgcg cgagttaacc accctgccct gcctggcagt aaaggacacg
    16681 aattctggaa acgagacttt acaggcagca gcgggctatt ttcctttgtg cttaagaaaa
    16741 aactcaatga tgaagagctg gcgaactatc tggataactt cagtttattc agcatggcct
    16801 actcgtgggg cgggtatgaa tcgttgatcc tggcaaatca accagaacat atcgccgcca
    16861 ttcgcccaca aggcgagatc gattttagcg ggaccttgat tcgcctgcat attggtctgg
    16921 aagatgtcga cgatctgatt gccgatctgg acgccggttt tgcacggatt gtataacatt
    16981 gccacttttg gacaattttg cagacatttt attgtgaaaa gtcttaaatt gttgcgtccg
    17041 ggatcaaggc gtcccggacg attcaggagt acaataggca gataaaggct taaacgctgt
    17101 tccacaggaa agtccatggc tgttattcaa gatatcatcg ctgcgctctg gcaacacgac
    17161 tttgccgcgc tggcggatcc tcatattgtt agcgttgttt actttgtcat gtttgccacg
    17221 ctgtttttag aaaacggcct gctgcccgcc tcatttttgc caggcgacag cttgttgata
    17281 ctggcaggcg cattgattgc ccaaggggtt atggattttc tgcctacgat tgcgattctg
    17341 accgccgcag caagtctggg ctgctggcta agttatattc aggggagctg gttagggaat
    17401 accaaaacgg tgaaaggctg gctggcacag cttcctgcta aatatcacca gcgcgccacc
    17461 tgcatgtttg accgccacgg tctgctggcg ctgctggctg gacgttttct tgcatttgtc
    17521 cgtacgctgc tgccaaccat ggcgggaatt tccggtctgc caaaccgccg cttccagttt
    17581 ttcaactggt taagtggatt gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta
    17641 agtatgattc cgttcgttaa acgccatgaa gatcaggtaa tgacgttcct gatgatcctg
    17701 ccaattgcct tgttaaccgc tggcttgtta ggcacgctgt ttgtggtgat taaaaaaaaa
    17761 tactgtaacg cctgacgatt ttccccgttc ccggctgctg taccgggaac gtatttaatt
    17821 cccctgcatc gcccgcattc ttgccgcatc ttcccccggc gtcacaccga agtaacgttt
    17881 aaactcacgg ctaaattgcg atgcgctttc atagccgacg cgcatcgctg ctgcgctggc
    17941 cttcatgccg tcatggatga tcatcatccg cgccttatgc agacggtaat tcttcaaata
    18001 ctgcaacggc gaggtgctgg tgacagactt aaaattatgg tggaacgccg atacgctcat
    18061 gttggcttct gccgccagtt gctcgacgct caggttttcg gtgtatttat tctcaattcg
    18121 tttcagcacg cggctaatca gactgaagtg agtctggcga ctgaccagcg ccagtaacgc
    18181 gccgccgcaa ggtccggtca gcacgtagta cagaatttcg cggatgatct gtttgccgag
    18241 aatacgcgca tccagtggtc gctccatcac gtcgagtaac cgctccgccg cgcataaaat
    18301 ctcttctgat aacgtggcgg agttaatccc gctggctgcc atcgacggct ggaaatgctc
    18361 atcttcgcca atgtccatca acagttcctg taactgcaaa atatcgacat tgagacgcaa
    18421 ccctgccagc ggcacctctg acgtggcata ggtttcgcac tcaaacggca acggcaccgt
    18481 cagcagcagg tattcattgg catcataacg aaacacgcgt tcattgatat aaccgatttt
    18541 atgcccggaa aagagaatta tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg
    18601 cgtctcgcca tacaacaaac gcacatcggg caacagttct gacaaactat tttctttatt
    18661 tttcagttta ttaactttat ccgccagcaa gcgacaaatc tcttcacgtt tcatatcgcg
    18721 taatttctta ggaataatgc ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc
    18781 actctggaga aataggcaag acattggcag aaatgagcat tgagagccag ggcgctggcg
    18841 atcacaatga aaaacatcag gcagatcgtt ctctgccctc atattggccc agcaaaggga
    18901 gcaagtaatg aacaacttta atctgcacac cccaacccgc attctgtttg gtaaaggcgc
    18961 aatcgctggt ttacgcgaac aaattcctca cgatgctcgc gtattgatta cctacggtgg
    19021 cggcagcgtg aaaaaaaccg gcgttctcga tcaagttctg gatgccctga aaggcatgga
    19081 cgtgctggaa tttggcggta ttgagccaaa cccggcttat gaaacgctga tgaacgccgt
    19141 gaaactggtt cgcgaacaga aagtgacttt cctgctggcg gttggcggcg gttctgtact
    19201 ggacggcacc aaatttatcg ccgcagcggc taactatccg gaaaatatcg atccgtggca
    19261 cattctgcaa acgggcggta aagagattaa aagcgccatc ccgatgggct gtgtgctgac
    19321 gctgccagca accggttcag aatccaacgc aggcgcggtg atctcccgta aaaccacagg
    19381 cgacaagcag gcgttccatt ctgcacatgt tcagccggta tttgccgtgc tcgatccggt
    19441 ttatacctac accctgccgc cgcgtcaggt ggctaacggc gtagtggacg cctttgtaca
    19501 caccgtggaa cagtatgtta ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga
    19561 aggcattttg ctgacgctaa tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta
    19621 cgatgtgcgc gccaacgtca tgtgggcggc gactcaggcg ctgaacggtt tgattggcac
    19681 tggcgtaccg caggactggg caacgcatat gctgggccac gaactgactg cgatgcacgg
    19741 tctggatcac gcgcaaacac tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga
    19801 gaccaagcgc gctaagctgc tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc
    19861 cgacgatgag cgtattgacg ccgcgattgc cgcaacccgc aatttctttg agcaattagg
    19921 cgtgccgacc cacctctccg actacggtct ggacggcagc tccatcccgg ctttgctgaa
    19981 aaaactggaa gagcacggca tgacccaact gggcgaaaat catgacatta cgttggatgt
    20041 cagccgccgt atatacgaag ccgcccgcta agctttttac gcctcaaact ttcgttttcg
    20101 ggcatttcgt ccagacttaa gttcacaaca cctcaccgga gcctgctccg gtgagttcat
    20161 ataaaggagg aacgtatggc taatccaacc gttattaagc tacaggatgg caatgtcatg
    20221 ccccagctgg gactgggcgt ctggcaagca agtaatgagg aagtaatcac cgccattcaa
    20281 aaagcgttag aagtgggtta tcgctcgatt gataccgccg cggcctacaa gaacgaagaa
    20341 ggtgtcggca aagccctgaa aaatgcctca gtcaacagag aagaactgtt catcaccact
    20401 aagctgtgga acgacgacca caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa
    20461 ctccagcttg attatatcga cctctactta atgcactggc ccgttcccgc tatcgaccat
    20521 tatgtcgaag catggaaagg catgatcgaa ttgcaaaaag agggattaat caaaagcatc
    20581 ggcgtgtgca acttccagat ccatcacctg caacgcctga ttgatgaaac tggcgtgacg
    20641 cctgtgataa accagatcga acttcatccg ctgatgcaac aacgccagct acacgcttgg
    20701 aacgcgacac acaaaatcca gaccgaatcc tggagcccat tagcgcaagg agggaaaggc
    20761 gttttcgatc agaaagtcat tcgcgatctg gcagataaat acggcaaaac cccggcgcag
    20821 attgttatcc gctggcatct ggatagcggc ctggtggtga tcccgaaatc ggtcacacct
    20881 tcacgtattg ccgaaaactt tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc
    20941 aaaattgcaa aactcgatca gggcaagcgt ctcggtcccg atcctgacca gttcggcggc
    21001 taacgtgcaa attctcccgg tggcggtaat gttccgctac cggacttttc agaaatcatt
    21061 tattcccctc gcgtcccgcc cgttgttact cttccttgtt caggaatgcc aaatataagg
    21121 acatcatcat gcagagccgg aagctcttaa aagaacaact catctatatc cgggataaac
    21181 gcaacggaga ggtgaaaaac agatgaaaat aatacttctg tttttagcag ccctggcaag
    21241 ttttaccgta cacgcgcagc ctccttcaca gaccgtagaa caaacagtcc ggcagattta
    21301 tcagaactat aaatcagatg ccagtacgcc ttatttcggt gaaaccggag agcgggcgat
    21361 aacttcagcg cgtattcaac aggcgcttac cctgaacgac aatcttacac taccgggcaa
    21421 tattggctgg ctggattatg atccggtttg tgattgtcag gattttggcg atctggtgtt
    21481 agaaagcgta gcgataaccc aaactgacgt cgatcatgcc gatgccgttg tgcgctttcg
    21541 tatctttaaa gatgataaag aaaagaccac acagacactg aaaatggtgg cggaaaatgg
    21601 tcgttgggtc attgatgata ttgtcagcaa tcatggcagc gtcttacaag cagttaatag
    21661 cgagaatgaa aaaacgctgg ccgctttagc ttcgttgcag aaagaacagc cagaatcctt
    21721 tgttgctgaa ctctttgaac atattgctga ttatagctgg ccgtggacgt gggtggtttc
    21781 cgactcttac cgccaggcgg ttaatgcctt ctataaaacc accttcaaga cggccaataa
    21841 tcccgatgaa gatatgcaaa tagaacggca atttatttac gacaatccga tctgttttgg
    21901 cgaagagtcg ctattttcac gcgttgatga aattcgagtc ctggagaaaa ccgccgattc
    21961 cgcccgcatt catgttcgtt ttacgctgac caatggcaac aatgaagagc aagagctggt
    22021 tttacagcgg cgcgaaggta agtgggaaat cgctgatttt atccgcccga acagcggcag
    22081 cctacttaag cagattgagg caaaaaccgc cgccagatta aagcaatgag ctgaattaaa
    22141 taacaattag ccggaacaat aaataaaagg gaacactata tgaaaacgat tttcaccgtg
    22201 ggagctgttg ttctggcaac ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat
    22261 caactggcga gcaatgtaca aacattaaat gccaaaatcg cccggcttga gcaggatatg
    22321 aaagcactac gcccacaaat ctatgctgcc aaatccgaag ctaacagagc caatacgcgt
    22381 cttgatgcac aggactattt tgattgcctg cgctgcttgc gtatgtacgc agaatgataa
    22441 aaaaatcccc ggcagcatgt cagttgccgg ggattttttt taacgtccaa ccgccgcttt
    22501 agggcgtttc ttcgcaccag cattcaccgg acgagattgc gtagacgacg ctttttttgc
    22561 cgtagcaggc gtctgacgct gagtcgccat cggcgtatgt ttagtcaacg ccggacgggt
    22621 attgcggttc tggcgacgag cttcacgcat ctcttcaatg gttggcgcag gcactaagca
    22681 atcgcgacgg ctgccaatca gatgcttttt gcccatcgct tccagcgcct ggcggattaa
    22741 cggccagttt gccggatcgt ggtaacgcaa caacgcttta tgcaaacgac gctgtttgtc
    22801 gcccttcggt acgaagacgc cttcgctctt ataaccaatc ttcgccagcg gttttttccc
    22861 ggtgtaatac atggtggttg agttcgccag cggcgatggg tagaagttct gtacctggtc
    22921 gagacggaaa cgatgctttt tcagccacag cgccaaattc accatatctt catcacgcgt
    22981 accggggtgc gcagagatga aatacgggat cagatactgc tctttacctg cctgtttcga
    23041 gtaggtatcg aacagctctt taaagcggtc atagctgccc atgcccggct tcatcatctt
    23101 cgacaacggc ccttcttcgg tatgttccgg agcaatcttc agataaccgc cgacgtgatg
    23161 ggtcgccaac tctttgatat agcgcggatc ttccacggcg atgtcataac gtacgcctga
    23221 ggcgataagg atttttttaa tgcctttcag atcacgcgca cggcgataga ggttgatcgt
    23281 cggttcgtgg ttagtgtcca tgtgcggaca aatatccgga taaacgcacg acaaacggcg
    23341 acaagtttgt tcagcgcgtg gcgatttgca gcgcaacata tacatgttgg cagttggccc
    23401 accgagatcg gaaatcacgc ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc
    23461 attaatgatc gaatcttccg aacggctctg aataatgcgc ccttcgtgct cggtaataga
    23521 acagaaagaa cagccgccaa agcagccgcg cataatgttg accgaaaaac ggatcatttc
    23581 gtaagccgga atacgggcat tgccataggc cggatgtggc acgcgcttgt acggcagcgc
    23641 aaaaacgctg tccatctctt cggtagaaag cgggatagca ggcgggttga tccacacata
    23701 gcggtcaccg tgtttttgca tcaatgcgcg ggcgcagcct gggttggttt cgtggtgcag
    23761 aatgcgcgaa gcatgggcgt acagcacttt atcgcccttc actttctcga aagaaggcag
    23821 caatacgtag gttttttccc acggtttctg gcgcggtggc tgcacgatta cagctttcgc
    23881 ttcctgcttt ttcggcgcaa caggtttgtt atccgcacac ggcaaatctt cgccatacgg
    23941 atgcgggatt gggtcgattt tccctagggt atcaagacgg gtggaatcca caccgctcca
    24001 gccaggcagc gcctctttta cgataattgc ggtattacgc acatcgcgga tttcactaat
    24061 cggctcgccc atcgccagac gatgcgctac ttccaccagc ggacgttcac cgttaccaaa
    24121 catcagcatg tcggctttcg aatctaccag aacggaacgg cgcacggtat cggaccagta
    24181 atcataatgc gcggtacggc gcaggctagc ctcaataccg ccgaggatca ccggcacatc
    24241 tttccacgcc tctttacaac gctgggtata aaccagcgtg gcgcgatccg gtcgcttacc
    24301 cgcgacatta tctggcgtgt aggcatcatc atgacgtaaa cggcgatcgg cggtatagcg
    24361 gttgatcatc gaatccatgt tgccagcagt aacaccaaaa aacagattcg gtttacccag
    24421 acgcataaag tcgtctttgc tgctccagtc cggctgggcg atgatcccga cgcgaaagcc
    24481 ctgcgcttcc agcatacgac cgcaaatcgc catcccgaag cttgggtgat cgacatacgc
    24541 gtcgccagta accaaaatga tgtcgcagct atcccagcca agttgatcca tctcttcacg
    24601 agacatcggc aaaaacggtg ccggtccaaa acaggcggcc cagtactgcg gccaggagaa
    24661 caggtcgcga tccggttgga tcagggagat agagctcatt ttgcttccag aaatgataaa
    24721 aaaataatca aaggccgggg attataagcc ggaacgaaag agaaatcgaa aggtattcca
    24781 tactcgccct cctcgggcga gtatgaagat tacggtaccg gattgaccaa cagttgccca
    24841 atcgagccac ggtccgccat ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc
    24901 caggaaggct gaccgaaata aacaagcagc tccacttgtc cgtcaaccca aacggtatct
    24961 ttccagcctc tgtcttccgg gaacggcatc gcgccattca cgttacggat ctggaacatt
    25021 acgccttcaa tatggaacgc ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt
    25081 ccttgctgcg cggtgacatc aatacggttg acgtcccaca gctgtccatt aatacccggg
    25141 tcatcaccca gactgatatc gcgactgcga attggcgaac cagccatgat ttcagttggc
    25201 agcaagcgca tcggaagact gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt
    25261 agcaccaagg tagaaaccag aatactggat ggctcaaaga agccgcgaat acgatcaaca
    25321 atgctcgccg cttcaccaca ggtgatcgac acttcatcgc cgttgctcat atccaccaga
    25381 atctcgcggc gctcgcccgg tgccagcgaa agttgcttca ccgatacagg agcaggcagg
    25441 aatccctgat cgccagaaat cacatgtaac gggcgaccat cgctcatctg tagttgatag
    25501 cgacgagagt tcgacgcgtt cagcagtcgc aagcgcaccc agccacgcga gacttcaacg
    25561 tacgggcttt gtacaccgtt aaccagcagc gtatcaccaa caaagccgcc gcttcccggt
    25621 tcgttgtatt ctggcgtacc aaagttatcc agccgtttat cctggataat gaccggaaaa
    25681 tcatccacac cataatggtt ggggataggc agcgacttgc tgacttcatc ttccaccagc
    25741 cacattccgg caaggccgtt atagacctgc tgagccgtgc ggttgggagt attggcgtga
    25801 taccacagag tagctgcgtt ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc
    25861 gacatcatcc gtgccggacc gcccatcagc gggcctggta cctgtagccc ggccaccgtc
    25921 attgagacat tttctgtcag gcggttgctg taaataagct taacatcgtc gcccttccag
    25981 acgcggatag tcggccccag gtaacgacca ttgattcccc agaccgacgc gcgtgtccct
    26041 ggcgtaaatg accagtgcgc acgttgtaca gtcataaaca gcggttgccc acggcgagat
    26101 tcaagtagcg gcggaacggg tagcggttgc tgttgcccgg ctgcgctggc cttcagggga
    26161 acagcgcctg cacaaagtgc aatccccgat gcctgaatga actgacgccg actgagtgac
    26221 atatttgctc catgtaaaac tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa
    26281 aatacagatt gccgacttaa acttttccgg cggcttcgcg ttctgcgact tctttatcga
    26341 gctcggcgat tttttgttcc attatcgaac gacaatgggc agccagctca cgaacctgat
    26401 ctttgccata ctgactgacg tcaattggcg gcagcatttc gacaatcacc agaccgttgt
    26461 gcagtcgatt aagattaatc ttattcgaag ttgtagagac gcacacggga ataatcggga
    26521 cgcccgccgc aattgccgcg tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac
    26581 ggctgcgggt tccttccggg aacatccaga tggaaatacg gcgttttttg aaatgattca
    26641 cgacttccgc aatggtgccg tgagctttag tgcgattgtt tctgtcgatc aataagttgc
    26701 cggttaacca gtacaactgc ccgaagaagg ggatccacag caagctcttt ttacctaccg
    26761 tcaccgtcgg cggttgcaca atgttcgatg ctgtcaccat gtcatagttg ttctggtggt
    26821 tagcgatata gatagcattg ccgtagcttt cagcatctgc aggtttgcgg cactcaactt
    26881 tcaggccgaa cagtggcgca agacggccaa acatatgccc aaaggtggcc acatgtttcg
    26941 ggttacgcgg gctgaaaagg cagtaaatgg agccgaatac acagactaag atgctgtaaa
    27001 tcacggtaat aataagacga aagatatata gcataacacc ctctgaaggt ctgagagact
    27061 ggcattgtac gtcacctgaa atcaggttag ggcattgtta aaagccgctc tctggaaaag
    27121 cgtattgtta atcgcaacgc atgaataaac aacggtttca agggaatttt tatcgtttcc
    27181 gggggttgaa tacgctgccg gatgacgact taacgtttca tccggcgttc cttgctagcg
    27241 ggaggaaaca gcgccctccc cggcatatta ctcttcgcta tcaccgctgc tggcacggcg
    27301 aggagagtcg atctcaacac gatcgatacg ctgcaaaccg cgcatcaacg taccgcggcg
    27361 tccacgttcg ccagtgactt tctgtagctc ttccggacgc agtttaattt tgcgtttccc
    27421 aacatgaatg gtcagcgtgc tttgcggcgg cagaacgtac agttgcgcca gaccatcctc
    27481 gccacgcgcg gcttctgccg atggaatgtt gataatcttg ttgcctttgc ccttcgacag
    27541 ctgcggcaga tcgcttaccg ggaacatcaa catacggcct gcctgagtga ttgccagcag
    27601 catatcggaa gcatcttcaa tcaccaccgg cggcataaca tgggcatttt ccggtaaggt
    27661 gatcaaagcc ttacccgcac ggttacgcgc taccagatcg ttaaaggtgc agacgaaacc
    27721 gtaacccgca tcggaagcca tcagcagttt ctgatcgtcg ctttccatca gcatatggtc
    27781 aacggtcgcc ccaggcggca acgttaattt gccggtgagt ggttcgccct gaccacgcgc
    27841 cgacggcagc gtaatcgggt cgatggcata gctacgaccg gtggaatcaa caaacactac
    27901 cggttggtta ctcttacctt tcaccgccgc tttgaagcta tcacccgctt tataattcag
    27961 gcccggcgcg tcgatatcat ggcctttagc gctgcgtacc cagcccatct gcgacagcac
    28021 aatggtgaca ggttcagacg gcagcatgtc gtgctcgctc atcgctttcg cttcttcgcg
    28081 ttcctgcaac ggcgaacgac gatcgtcacc gtaggcttgc gcgtctgcct gcagttcttt
    28141 cttcagcagg ttattcattt tacgctcgga agccaaaatg ccctgcaact ggtcgcgctc
    28201 tttttccagc tcactctgct caccgcgaat cttcatctct tccagtttgg caagatgacg
    28261 cagtttcagt tcgaggatcg cttccgcctg ggtttccgta aggccaaacc gcgacatcag
    28321 cgccggtttc ggttcatctt cgttacgaat gatctcaatc acttcgtcga tgttgagaaa
    28381 cgccaccagc aaaccttcca ggatatgcag gcgcttgagg actttctcca gacgatagtt
    28441 cagtcggcgg cgcacggtat cgcgacggaa caccagccat tcggagagga tttccagcag
    28501 gtttttcacc gccggacgac catccagacc gatcatattg aggttaatgc gatagctctt
    28561 ttccagatcg gtggtagcga agaggtggtt catcacctga tccatatcca cgcggttgga
    28621 acgcggcaca atcaccagac gggtcgggtt ctcgtggtca gattcatcgc gcagatcgtc
    28681 aaccatcggc agctttttgt tgcgcatttg cgcagcaatt tgctccagta cgcgcgcacc
    28741 tgaaacctga tgcggtaatg cgctgataac caccgcgcca tcttctttct tccacaccgc
    28801 gcgcatacgc actgaaccac gtccgttctc gtagatttta cgaatctcgg cgcgcgaagt
    28861 gataatttcc gcttcagtcg gataatccgg cccctgcacg atatccagca gctgatcgag
    28921 cgtagttttc ggctggtcga ttaatgcgat tgccgcctga gctacttcac gcaggttatg
    28981 cggtggaata tcggtcgcca tgccgacggc aataccggtg gtgccgttaa gcaaaatgtt
    29041 tggcagacgg gcaggtagca ttttcggctc ctgcaaagtg ccgtcgaagt ttggcaccca
    29101 gtcagccgtc ccctgcccca gctcgctcaa tagcagctcg gaatatttcg acaaccggga
    29161 ttcggtgtaa cgcattgccg cgaacgattt cggatcgtcc ggcgcgcccc agttcccctg
    29221 accatcaacc agcggataac ggtaagagaa cggctgcgcc atcaggacca tcgcttcata
    29281 acaggcgcta tcgccgtgcg gatggtattt acccagtacg tcaccgacgg tacgggccga
    29341 ttttttaaat ttggcgctgg cattcaggcc cagttcagac atcgcataca caatgcggcg
    29401 ctgaacaggt ttcagaccat caccaataaa cggcaacgca cggtccatga tcacgtacat
    29461 ggagtagttt aagtaggcgt tttccgtaaa ttcatgtagc gcaaggcgct ctgccatatc
    29521 gctcattaat tctgattcct caacttattc gccagccttc aaaggggcaa tattgccgga
    29581 gatactacat tatctgccgc gtcgagtcac aggagatcag cgtgaatagc gccgccggac
    29641 gtgctccgac ggcgataaag gcatcaatgt gccttgatat acaactcttt gctgtagact
    29701 tgatcggcat agagggcagc acaaggttgc gcccatgcca caccactcgg catgggggtc
    29761 cgctccgggc gattcgagaa gttgaggtta tgcgagtcca gggaagccta aagcatcaaa
    29821 gtagtcgact gtaagtatac ggttcagccc agtttagctg tgtctccacc agtgccccgc
    29881 gcctcctgcc ataagtgcgg cttgctcatg gatatccccg agacggctta cggcgcaccg
    29941 gataataaat ttcgcgtata catggtattc cttaccggag aaattataag cccaacaaca
    30001 atacgcctgt gacattgttc gctcaaactt atcgcgagtg atttggctca cactccggta
    30061 aagattcccc atcacgccaa cggcacataa acatccgttt ccagtcgctg ctctgtcacc
    30121 ccttcggcaa gattggtgta gtgaaacaga atcggtgctt tacgcatttt ctcgccgctt
    30181 gcaggcagcc agtggcgaat aatgccccat atcgtgtgtg aaatatcgtc cagctcgcca
    30241 acgtggcggg ccacggcata acgtccaccg gtaagttcac cattgctcac accataacga
    30301 ttatcgggaa tcggttcgct aacgctgccg cagatatcaa agcgaaacgc ttccggtgcg
    30361 gtggtggcag gggcgtccca ggcgacgcca aaagtctggc tttggttaac aggagaaagc
    30421 cccgtctctt tacgccacat gataaatttc gccacactgt agttgagcaa ctcagggcta
    30481 cagcggtgcg gcaacatcgc cacagggatt gatgggaaat cgataatgtt tacatccagt
    30541 gtcaggtttg tcattttgcc tccctgcgaa attattcccg ggaacgcgtc ccgggagcgg
    30601 taacaattac ggatttactt tgcggatctg tttgacgtca atttcaacag aattccagtc
    30661 tttatcgact tcaccctgaa tctcaaccgt atctttcggc gtcaccgtca caccgttcca
    30721 gcgtttgtga tcgatatcaa cattgatagt accgctggca tctttaaaca cgtagagatc
    30781 gtcagagatg cgttcaacga tattgccgcg cagggttacc caggtgtcgt cacgcaggga
    30841 ttttgcgctt tctacagtcg ttacgctgcc gttcggcccc tgaaatcctc cggcctgact
    30901 ttgcgttacc gatgggccag aaaaaccgcc ctgctctgct gccatcaccg gtgcactgca
    30961 cagggccatt actgcgatta ctgctgcgaa ttttttcatg tttattactc cctttaatgt
    31021 ctgtttccga gcatttaaca agatagtcct taacaacttc ttaagggaaa aaaataaaat
    31081 ttaatgctgt acagagcgcg ttacaacacg gtttactggc agcaaatacg gttatcgcag
    31141 ggatgaaaaa atgcgaattt tactgataga agatgacatg ctgattggtg acggcatcaa
    31201 aactggcctt agtaaaatgg gttttagtgt cgactggttt acacaaggtc gtcagggaaa
    31261 agaggcgctt tatagcgcac cttatgatgc ggtgatcctg gatttaacct taccaggcat
    31321 ggatggtcgc gatattttgc gcgaatggcg agagaaaggt cagcgtgagc cggtactgaa
    31381 cctgaccgcg cgtgatgcgt tagcggaacg tgtagaaggg ctgcgtctgg gagctgacga
    31441 ttatctgtgt aaaccttttg cgttgataga agtcgccgcc aggctggagg ctctgatgcg
    31501 ccgaaccaac ggccaggcca gcaacgagct gcgccacggt aacgtcatgc tcgaccccgg
    31561 caaacgtatc gccacgctgg ctggcgaacc cttaacactg aaaccaaaag aatttgccct
    31621 gctggaatta ctgatgcgta acgctggtcg ggtactgccg cgcaaactga ttgaagagaa
    31681 actgtatacc tgggacgaag aggtcaccag taatgccgtt gaagtgcatg tgcatcatct
    31741 gcgacgcaaa ctcggtagtg attttattcg taccgtgcat ggtattggct acacattagg
    31801 tgagaaatga aatttaccca acgtcttagt ctgcgcgtca ggctgacgct aatcttttta
    31861 attctggcct cggtgacctg gctgctttcc agctttgtcg cctggaaaca aacaacggat
    31921 aacgtcgatg aattgttcga cacccaactg atgctgtttg ccaaacggtt aagtacgctc
    31981 gatctcaacg aaatcaacgc ggcggatcgc atggcacaga cgccaaataa attaaaacac
    32041 ggtcatgttg atgacgatgc gctgaccttt gccatcttta cccacgacgg cagaatggtc
    32101 cttaatgatg gcgataacgg agaagatatt ccctatagct atcaacggga aggttttgct
    32161 gacgggcaac tggtcggtga agacgatcct tggcgttttg tctggatgac ctcacctgat
    32221 ggcaaatatc gcatcgttgt tggccaggaa tgggaatacc gtgaagacat ggcgctggcg
    32281 attgttgccg ggcaattgat cccgtggctg gtcgcactgc cgattatgtt aatcatcatg
    32341 atggtactac tgggtcgtga actcgcgccg ctgaacaaac tggcgctggc actacgtatg
    32401 cgtgaccctg actcggaaaa accactaaac gcgactggcg tacccagcga agtgcggcca
    32461 ctggttgagt cgctaaatca actgttcgcc cgcacacatg cgatgatggt tcgagaacga
    32521 cgctttacct ccgatgcagc tcacgaacta cgtagcccgt taacagcgct gaaagtgcaa
    32581 accgaagttg cgcagctctc tgacgatgat ccgcaggcgc ggaaaaaagc actgctccaa
    32641 ttacattccg ggatcgatcg cgctactcgt ctggttgatc aactgctcac gctatcgcgg
    32701 ctggactcac tggataacct tcaggacgtc gcggagatcc cgcttgaaga tctcctacaa
    32761 tcgtcggtga tggatattta ccacacggcg cagcaggcga aaattgacgt gcgactgaca
    32821 ctcaatgccc acggcatcaa acgcaccgga caaccgctat tgctaagttt gttggtgcga
    32881 aatttgctgg ataacggccc cggtgtgaca ccagaggcac tggcgcgaat tggcgaacgc
    32941 ttctatcgcc cacccggaca aaccgctacc ggcagtggac ttgggctatc gattgtccag
    33001 cgaatcgcca agctgcatga catgaatgtt gagtttggga atgcggaaca aggtggattt
    33061 gaggcgaagg taagctggta acttctctgt ctatgagagc cgttaaaacg actctcatag
    33121 attttactaa tagcaaaata taaaccgtcc ccaaaaaagc caccaaccac aaaccagaca
    33181 aataacgagc gagaatacat atagccaaaa ctcgaacatt tcccgcataa agagttttct
    33241 taagataaga ataataagtg gcgtaagaag aaaaaatgct gcaatcattg cttgcaatat
    33301 acgatcttcg cctgaaacgg aaggtatact gcaaatattg cctcccgact caagcatcca
    33361 gtcatactta ttgaaagcaa aaaaagaatc tattaaccaa taagtaagca ttatatatgg
    33421 caatgcttca aatattgttt taattttc
//
LOCUS       DAZOYF010000027        33264 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZOYF010000027 DAZOYF010000000
VERSION     DAZOYF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.027"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..190
                     /locus_tag="ACHUOF_001239"
     CDS             <1..190
                     /locus_tag="ACHUOF_001239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610500.1"
                     /translation="PPHVKALPDTITQTSIHRTLQFIEFARSCRPDKAFTPHPAINRR
                     RLPNGRFFTYSPPSNVPA"
     gene            complement(156..1391)
                     /locus_tag="ACHUOF_001240"
     CDS             complement(156..1391)
                     /locus_tag="ACHUOF_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0610501.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="ACHUOF_001241"
     tRNA            complement(1538..1614)
                     /locus_tag="ACHUOF_001241"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="ACHUOF_001242"
     tRNA            complement(1657..1743)
                     /locus_tag="ACHUOF_001242"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="ACHUOF_001243"
     tRNA            complement(1764..1839)
                     /locus_tag="ACHUOF_001243"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="ACHUOF_001244"
     tRNA            complement(1898..1974)
                     /locus_tag="ACHUOF_001244"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="ACHUOF_001245"
     CDS             complement(2077..3462)
                     /locus_tag="ACHUOF_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0610502.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHUOF_001246"
     CDS             complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHUOF_001246"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR0610503.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHUOF_001247"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHUOF_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR0610504.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHUOF_001248"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHUOF_001248"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR0610505.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHUOF_001249"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHUOF_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR0610506.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHUOF_001250"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHUOF_001250"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR0610507.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHUOF_001251"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHUOF_001251"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR0610508.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHUOF_001252"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHUOF_001252"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR0610509.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHUOF_001253"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHUOF_001253"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR0610510.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHUOF_001254"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHUOF_001254"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR0610511.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHUOF_001255"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHUOF_001255"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR0610512.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHUOF_001256"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHUOF_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR0610513.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHUOF_001257"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHUOF_001257"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR0610514.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHUOF_001258"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHUOF_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR0610515.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHUOF_001259"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHUOF_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR0610516.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHUOF_001260"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHUOF_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR0610517.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHUOF_001261"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHUOF_001261"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR0610518.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="ACHUOF_001262"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="ACHUOF_001262"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR0610519.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21650..23671)
                     /gene="rep"
                     /locus_tag="ACHUOF_001263"
     CDS             complement(21650..23671)
                     /gene="rep"
                     /locus_tag="ACHUOF_001263"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR0610520.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23758..24039
                     /gene="ppiC"
                     /locus_tag="ACHUOF_001264"
     CDS             23758..24039
                     /gene="ppiC"
                     /locus_tag="ACHUOF_001264"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR0610521.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24085..25560)
                     /gene="ilvC"
                     /locus_tag="ACHUOF_001265"
     CDS             complement(24085..25560)
                     /gene="ilvC"
                     /locus_tag="ACHUOF_001265"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR0610522.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25710..26600
                     /gene="ilvY"
                     /locus_tag="ACHUOF_001266"
     CDS             25710..26600
                     /gene="ilvY"
                     /locus_tag="ACHUOF_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR0610523.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26597..28141)
                     /gene="ilvA"
                     /locus_tag="ACHUOF_001267"
     CDS             complement(26597..28141)
                     /gene="ilvA"
                     /locus_tag="ACHUOF_001267"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR0610524.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28144..29994)
                     /gene="ilvD"
                     /locus_tag="ACHUOF_001268"
     CDS             complement(28144..29994)
                     /gene="ilvD"
                     /locus_tag="ACHUOF_001268"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR0610525.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30059..30988)
                     /gene="ilvE"
                     /locus_tag="ACHUOF_001269"
     CDS             complement(30059..30988)
                     /gene="ilvE"
                     /locus_tag="ACHUOF_001269"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR0610526.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31008..31271)
                     /gene="ilvM"
                     /locus_tag="ACHUOF_001270"
     CDS             complement(31008..31271)
                     /gene="ilvM"
                     /locus_tag="ACHUOF_001270"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR0610527.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31268..32914)
                     /gene="ilvG"
                     /locus_tag="ACHUOF_001271"
     CDS             complement(31268..32914)
                     /gene="ilvG"
                     /locus_tag="ACHUOF_001271"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR0610528.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32917..32967)
                     /gene="ilvX"
                     /locus_tag="ACHUOF_001272"
     CDS             complement(32917..32967)
                     /gene="ilvX"
                     /locus_tag="ACHUOF_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR0610529.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33053..33151)
                     /gene="ilvL"
                     /locus_tag="ACHUOF_001273"
     CDS             complement(33053..33151)
                     /gene="ilvL"
                     /locus_tag="ACHUOF_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR0610530.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8034 a         9213 c         8407 g         7610 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag gtgccggatg cgatgctgac gcatcttctc cggccttgat tatttccctc
    21661 gttttgccgc catcatcgct ttcagattcg ccagatggct ttgccctttc tgcatccgtt
    21721 cttcggcact gaccactttg cgctcctgtt cccaaatcag atcatcctgc ggcagctcca
    21781 gcaaaaagcg gctcggctcc gggcgcacca gttcgccgta ctgacggcgt tctttacaca
    21841 gcgtaaaggt caattccttc tgggcgcggg taatgccgac ataggccagc cgccgctcct
    21901 catcgatatt atcttcatcg atactgctct ggtgcggcaa aaacccttct tccataccga
    21961 ccatgtagac ataaggaaac tccagccctt ttgacgcgtg gagagtcatc agttgcacct
    22021 gatccagctc ttcttcactc tcaccacgct ccatcatgtc gcgtaaagta aagcgcgtca
    22081 ccacctgggt gagcgtcatc ggctcatcca gttcgctgcc ttccagcatc tccgtcatcc
    22141 agctaaacag ttggttgacg ttcttcatgc gcatttcggc agctttcgtg ctgggcgatg
    22201 tttcgtacag ccaggattca taatccatgc cgtggatcag atcgcgcacc gcggcaatcg
    22261 gctcccgctc cgccagacgc tggatttctg ccaaccagtg agtgaagcgg gtcaatgctt
    22321 cataaccacg tccgctaagc gtctgactca ggcccatatc aaagctggcg gtaaacatgc
    22381 ttttattgcg cgtcatcgcc cactcaccca gctttttcag cgtcgccggg ccaatctctc
    22441 gcttcggcgt gttaacgata cgcagaaatg cgctgtcatc gtccgggtta gtcagcacgc
    22501 gcagataagc cagcaagtcc ttgatttcag gacgagagaa aaacgacgta ccaccagaaa
    22561 ttttgtacgg gatgcggttt tgcatcagga atttttcaaa cacccgcgac tgatagttac
    22621 cgcgataaag aatagcgtaa tctttgtact gcgttttatt gacgaagtga tgcgcaatca
    22681 gttcaccagt gacgcgctcg gcttcatgtt cttcgttatt cgcgcttaaa acttttagct
    22741 ctgtgccgta acccagttcg gagaacagac gcttttcaaa gacgtgcggg ttattggcga
    22801 tcaggatgtt cgccgctttc aaaatacgtc cggaagagcg gtagttctgc tcaagcttaa
    22861 tcaccttcag cgccggaaaa tcctggctca gcagcaccag gttttgcgga cgtgcgccgc
    22921 gccaggagta gatcgactgg tcatcgtcac ccaccacggt aaagcgcgcg cggctgccca
    22981 ccagcagttt caccagttca tactggctgg tgttggtgtc ctgatattcg tccaccagca
    23041 ggtagcgaat tttgttctgc cagcgctcgc ggacttcttc attgcgttgc agaagtagcg
    23101 tcggcaataa aatcagatca tcgaagtcga gcacattaca ggctttcagg tgtgcatcat
    23161 acagcccata acaatgggca aaaatacggt cccgctcgcc aatcgcactg gctgccgcct
    23221 gggacggtgt tttgagatcg ttcttccagt tagagatggt cgaaatcaac tgttgcagga
    23281 gaactttgtc atcttcaatc agcccttcgg tcaactcttt gagcaacgcc agctgatcgg
    23341 tatcgtcaaa cagcgaaaag ttcgccttca tccccagcgc cgcatactca cgcttgatga
    23401 tatccagccc cagcgtgtgg aaggttgaga tcatcagccc atgcgcctct ttgcgaccca
    23461 gcgtctgtcc gacacgctct ttcatctcgc gcgccgcctt attggtaaag gtcaccgccg
    23521 cgatatgtcg cgcctgataa ccgcacccgc ggatcagatg ggcgatttta ttggtgataa
    23581 cacgagtttt accggaaccc gcacccgcca gcaccaggca ggggccggta acgaattcga
    23641 cagcttgttg ttggccgggg tttagacgca taggaaaatc gctcaatgaa agtcgggagg
    23701 ggaaaataac agcgtggtag tatagccagc ctcatcctta ccactcaagg cacgatcatg
    23761 gcaaaaacag cagcagcact gcatatcctt gtaaaagaag agaaactggc tctggatctt
    23821 ctcgtgcaga ttaagaacgg tgccgatttc ggcaagctgg cgaagaaaca ctccatttgc
    23881 ccatcaggca aacgcggcgg tgatttaggt gaattccgcc agggtcagat ggttccggcg
    23941 ttcgataaag ttgtgttctc ttgtccggta ctggagccaa ccggcccgct gcacacccag
    24001 ttcggttacc acatcattaa ggtgctgtac cgtaactaat agcaaggcct tctccgggag
    24061 aaggccttga actctgaatc gctattaacc cgcaacagca atacgtttca tatccgtcat
    24121 atagccgcgc agtttcttac ctacttgctc aatcgcatgg ctgcgaatcg cttcatttac
    24181 atcacgcagc tgcgcgttat ctaccgcacc ttccggaata gctttaccca aatcgcccgg
    24241 ttgcagttct gccataaacg gtttcagcag cggcacacaa gcgtaagaga acagatagtt
    24301 accgtactcg gcagtatcag agataaccac gttcatttca tacagacgct tacggacgat
    24361 ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt cttcgatgat
    24421 gccggaatca accatggttt cgaacgccag ttcaacgccc gctttcacca tcgcgatcat
    24481 cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact gcggcgcggt
    24541 ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat catcgttggc
    24601 ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca tatgtttctg
    24661 gaacagcggt gccatgatct ctttcagctg ttcagacaac gcgtaagcac gcagtttcgc
    24721 cgggttagag agacggtcca tcatcagtgt gataccgccc tgcttcagcg cttcggtgat
    24781 ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc cctcttccac
    24841 cagcttgtcg aagcacagca gcgagccagc ctgcaacata ccgcacagga tggtttgctc
    24901 acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc ccgcacggtg
    24961 accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt tcggatcgtt
    25021 ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact cttcacgcac
    25081 ttcggtgcca gggcatttcg gcgcaaccat gactacggtg atgtctttac ggatctgctc
    25141 gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt ctttcatcag
    25201 tggctgtacg gtgcgcacta catcagagtg ctgcttgtcc ggcgtcaggt taaccaccag
    25261 atccgcctgt gggatcagtt cttcgtaagt acccacttta aaaccatttt cggtcgcttt
    25321 acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg agatatcgag
    25381 accagaatca cgcatgttca gaccctggtt cagaccttgt gcgccacagc cgacgatgac
    25441 tactttttta ccctgaaggt agctcgcgcc atcggcgaat tcatcgcgcc ccataaagcg
    25501 acatttgccc agctgtgcca gctgctgacg cagattcagt gtattgaagt agttagccat
    25561 ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga gattgaattc
    25621 actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac attgcaattt
    25681 ctgcaacgtc aacattgagg gctgcgccat tggatttacg cgatctgaaa accttcctgc
    25741 atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt agcccgtcca
    25801 cgctctcacg gcagattcag cgcctggaag aagatctcgg ccagccgctg tttgtgcgcg
    25861 ataaccgcac ggtgacgctg actgaagcgg gcgaagaact gcgcgttttc gcccagcaaa
    25921 cgctgttgca gtatcagcag ctgcgccaca ccatcgacca gcaagggccg ttgctctatg
    25981 gcgaattaca tatcttctgc tcggtgaccg ccgcttacag ccatctgccg ccgattctgg
    26041 atcgcttccg cgcggaacac ccgtcggtgg agatcaaact cactaccggt gatgcggcag
    26101 atgcaatgga aaaagtcgtc acaggtgaag ctgatctggc gattgccggg aagccagaga
    26161 ctctgcccgg cgcggtggcg ttttcgatgt tggagaatct ggcagtggtg ttgattgcac
    26221 ccgcattgcc ctgcccggtg cgtaatcagg tttctgtcga aaagccggac tggtcaacgg
    26281 taccgtttat catggccaat caggggccgg tgcgccgccg cattgagttg tggttccgac
    26341 gcaataaaat cagtaacccg atgatttacg ccacggttgg cgggcatgaa gcgatggtat
    26401 cgatggtggc gctcggctgt ggcgtggcat tgttgccgga agtggtgctg gaaaacagcc
    26461 ccgaaccggt gcgtaaccgc gtgatgattt tagagcgcag cgatgagaaa acgccgtttg
    26521 agctgggcgt gtgtgcgcag aaaaagcggc tacatgagcc gctaattgag gcattctgga
    26581 agattttgcc gaaccattaa cccgccaaaa agaacctgaa cgccgggtta ttggtttcgt
    26641 cgtggcaatc gtagcccagc tcattcagcc gggtttcgaa atccggttca tggtcgccaa
    26701 gttcgaacgc cgccagtacg cgcccgtagt cggtgccatg gctgcgatag tggaacaaag
    26761 aaatgttcca gtgcgtaccc agcgtgttga ggaagcgcag cagcgcgccc ggtgattccg
    26821 ggaattcgaa gctgtagagg cgttcctgca acggatgtga cggacgcccg ccgaccatat
    26881 agcgcacatg caacttcgcc atttcgtcgt cggagagatc caccacgctg tagccgccgt
    26941 cgttgagcat ctgcaaaatt tctttgcgct cttcgaggcc gcggctcagg cgcacgccga
    27001 caaagatgca ggcgtttttg gcatcggcaa aacggtagtt gaactcggtg accgatcgcc
    27061 cgccaagcag ttggcagaat ttgaggaagc tgcctttttc ttccggaatg gtcaccgcca
    27121 acaacgcttc acgctgttcg cccagttcgc agcgttccga gacgtagcgc aggccgtgga
    27181 agttcacgtt ggcaccggaa agaatatgcg ccagccgttc gccgcgaatg ttgtgctggg
    27241 cgatatattt tttcattccc gccagcgcca gcgcgccaga gggttccgcc accgcacgca
    27301 catcttcgaa taaatccttc atcgccgcac agatcgcatc gctatcgacg gtgatgatgt
    27361 cgtcgagata ctcctggcat aaacggaagg tttcgtcacc gatgcgtttt accgcgacac
    27421 cttcggcgaa cagtcccacg cgtggcagat caaccggatg acccgcatcc agcgctgctt
    27481 tcaggcaggc ggagtcttcc gcttctacgg cgatcacttt gatttgcggc atcagttgtt
    27541 tgatcagcac cgctacgccc gccgccagac cgccgccgcc gactggcaca aatacgcggt
    27601 cgagatgggc gtcctgctgg agcagttcca gcgccagcgt gccttgcccg gcaatcacca
    27661 tcggatggtc gaacggcggc acccaggtga acccctgctg ctgtgacagt tcgatcgctt
    27721 tagctttcgc ttcgtcaaag ttcgcgccat gaagcagcac ttcgccgccg aatccacgta
    27781 ccgcatcgac tttgatgtcg gcggtggcgg ttggcataac gatcagggcc ttcacgccta
    27841 accgcgcaga agaaaacgcg acgccctgcg cgtggttacc cgcagaagca gtgatcacgc
    27901 cgtgcgcttt ttgttcttcc gtcaggcccg ccatcatggc gtatgcgccg cgcagcttaa
    27961 agctgtgcac tggctggcga tcttcgcgct tcaccagaat gacgttatca agacgcgacg
    28021 acagtttttc cattttttgt agcggcgtaa cctgcgccgc ctcgtaaacc ggcgcgcgca
    28081 gcactgctct taaatattcg gcaccttccg gagcaccgga caggggttgc gagtcagcca
    28141 ttattaaccc cccagtttcg atttatcgcg caccgcacct ttgtcggcgc tggttgccag
    28201 gctggcgtag gcgcacaggg caaaggaaac ctgacgttca cggtttttcg gcgtccaggc
    28261 tttgtcaccc cgagcttcct gcgcttcacg acgcgccgcc agttcggcat cgcttacctg
    28321 taactgaatg ccacggttcg ggatgtcgat agcgatcagg tcaccatctt caatcagacc
    28381 aatgctgccg ccgcttgccg cttccggtga gacgtggccg atggaaagac cagaggtgcc
    28441 gccagagaaa cgaccgtcgg tgatcagcgc acaggcttta ccgagaccca ttgatttcag
    28501 gaagctggtt gggtagagca tttcctgcat gccagggccg cctttcgggc cttcatagcg
    28561 gatcaccacc acatcgcccg cgacaacttt gccaccgagg atcgcttcta ccgcgtcgtc
    28621 ctggctttcg tacactttcg ccgggccggt gaatttgagg atgctgtcat cgacgcccgc
    28681 ggttttcacg atgcagccgt tttcagcaaa attaccgtag agcaccgcca ggccgccgtc
    28741 tttgctgtag gcgtgttcca gcgagcggat acagccgttt gcgcgatcgt catcgagaga
    28801 atcccaacgg caatcctgcg agaatgcctg tgtagtacga atgcccgcag ggcctgcgcg
    28861 gaacatattt tttaccgcgt catcctgggt cagcataacg tcgtattgtt ccagagtttg
    28921 cggcaacgtc aggccaagta cgtttttcac atcacggttc agtaaccccg cgcgatccag
    28981 ttcaccgaga ataccgataa caccaccagc acggtgaaca tcttccatat ggtatttttg
    29041 ggtgctcggc gcaactttgc ataactgcgg gactttacgc gaaagtttgt cgatatcact
    29101 catggtgaag tcgatttccg cttcctgcgc cgccgccagc aggtgaagta ccgtgttggt
    29161 agagccgccc atcgcgatat ccagcgtcat ggcattttca aacgccgctt tgctagcaat
    29221 attgcgcggc agtgcacttt cgtcgttttg ctcgtagtaa cgtttggtca attcaacaat
    29281 gcgtttaccg gcattgagga acaactgctt acggtccgcg tgggttgcca gcagcgagcc
    29341 gttgcccggc tgcgacaggc ccagcgcttc ggtcaggcag ttcattgagt tagcggtaaa
    29401 catcccggag caggagccgc aggtcggaca cgcggaacgt tcaacctgat cgctctggga
    29461 gtcagaaact ttcgggtctg cgccctggat catcgcatca accagatcga gtttgattat
    29521 ctgatcggac agtttggttt tcccggcctc catcgggccg ccggaaacaa agatcaccgg
    29581 aatattcagg cgcagggaag ccatcagcat ccccggggtg attttgtcgc agttagagat
    29641 gcagaccatg gcgtcggcgc agtgggcgtt gaccatatac tcaacggaat cagcgatcag
    29701 ttcgcgagat ggaagtgaat aaagcatccc cccgtggccc atggcaatcc catcatccac
    29761 cgcaatggtg ttgaactctt tggcaacgcc gccagccgct tcaatttgtt cggcgaccag
    29821 tttaccgaga tcgcgcagat ggacgtgacc cggtacaaat tgggtgaacg agttcacaac
    29881 cgcgataatc ggcttgccaa aatcggcgtc ggtcattccg gtggcgcgcc acagcgcacg
    29941 agcacccgcc atattacgac catgagtggt ggtggcggaa cggtacttag gcatacttta
    30001 tttactccca gtgtctgtct cgtaaatggg acggtgcgtg ccgtcccatt ttttgtattt
    30061 attgattaac ttgatctaac cagccccatt tatcttcggt ttcgccagtg aagaggccga
    30121 agaaggcttg ctgaatgcgt ttggtaaccg ggccacaacg gccttcgcca atctgaatac
    30181 cgtctacgct gcgcactggc gtgatttctg ccgccgtacc ggacataaag acttcatccg
    30241 ccaagtacag ggattcgcgc gacagcacct gctcacgcac ttcaattccc agctctttcg
    30301 ccagtttgat gatggcatca cgggtaatac ccggcagcgc ggaggaggtg aacggtgggg
    30361 tgaacagcac accatctttc acttcaaaca ggttttcgcc tgcgccttca gagatataac
    30421 cgttcacatc cagcgcgata ccttcctgat aaccgtggcg gcgcgcttcg ctacccacca
    30481 gcagggaaga gagatagtta ccaccggctt ttgccgcagt tgggatggtg tttggtgctg
    30541 cgcggttcca ggaggaaacc atcgcatcga tcccctgctc cagcgcttct gcgcccagat
    30601 acgctcccca cgggaaagcg gcgataatca catcggttga gtatcccgct ggcgggttta
    30661 ctcccatgcc aacatcaccg acgaagatca gcggacggat ataggcgctg gtgagattgt
    30721 ttttgcggat cacgtcacga caagcttcca tcagctcatc aatgctctgt gaaaccggga
    30781 agcgatagat tttggcggag tcatgcagac gctgcatatg ctcacgatgg cggaatacaa
    30841 ccggtccttt gtgtgagtcg tagcaacgga tgccttcaaa aaccgaggtg ccatagtgca
    30901 gcgcgtgcga catcacatgc accttcgcgt cttcccagcg aaccatctcc ccattgaacc
    30961 aaatgtaatc agctttcttc gtggtcattt ttatattcct tttgcgctca ggcgcggatt
    31021 tgttgtgatg tggttgtgct ctggcagatg gcaacgtgtg cgacgtccac cagtttattt
    31081 aactgactaa acagtaagtc gaccgaccgt gggctggcaa cggtcaattc gatatttata
    31141 ttttgtgcat cgctggcggc agccatattc attgagcaga cgtggaaacc acgatgacgc
    31201 accacgcgta aaacacgttc taaggtttcc ggattgaagc gagccgatac attgacctga
    31261 tgttgcatca tgataatttc tccaacattt ctgaattact ggcacctggc ggcaccagcg
    31321 gccagacgtt ctcaagttcg tcgattgaga catgaagcag gtatggccca tcactgttca
    31381 gcatggtgtc gagtgccgct tcaacctggt ctttacgggt gatgtgttgg ccagggatgc
    31441 cgaaggcgct ggctaacatg aggaaatcgg ggttatcggt aagggtggtt tcgctgtatc
    31501 gttcctgaaa aaacagttgc tgccattgtc gaaccatccc taaccgttgg ttatcgagta
    31561 agacgatttt caacggtaac tgcttgcgtt ttacggtgcc cagctcttgc acattcatca
    31621 tgaaagagcc gtcaccggag atacagacaa cggtatcgtt cggtcgcgcg acttgtgcgc
    31681 caactgccgc cggtaaacca aaacccatgg tacctaagcc gctggaggtg atgaaatttt
    31741 ccgggcgagt gtgggcgatg tgctgcgcag cccacatctg gtgctgcccc acatctgtgg
    31801 tcacgacgca atccgcaggt ttacgatccg acagttgttt taacaacaac ggagcgtaga
    31861 tagcgtcacc gggatggtcg taacgccagg aatgttcatc acgcagctgc gcgcagtgtt
    31921 gctgccagtc attgatattt aacggctgct gtaatgctgg taacagagca tttaaatcac
    31981 cttgtaatgc cacatgtgcc tgacgcagct tgttcatttc tgccgggtcg atatccatat
    32041 ggataacact ggcgtgtggt gcgaaggtgt tcagtttgcc ggtcacccgg tcatcaaaac
    32101 gtgcacccac ggcgatcagc aggtcacact cctgcaccgc gaagtttgcc gctttggttc
    32161 cgtgcatccc cagcatgccc agatagtacg gataatctgc ttctactgcg cccagccctt
    32221 tcagcgtaca ggtggcaggc atttttgtgg cagcgagaaa ttcacgcaaa gccggaactg
    32281 cctgcgccat ccccacgcca ccgccaacgt acagcatcgg tttttgcgct tttgccagca
    32341 tctggcgcgc ttgctcgact tcggcatgtg ggaaagtcac ttcgttttca acggtggtga
    32401 accacggttc cagatcgccg ctggctaact ggatatcttt tgggatatcg accagaaccg
    32461 gaccaggacg acctgagctg gcaacgtcga atgcttcagc catgatgcgc ggcaactctt
    32521 ccagagactg caccagaaag ctgtgcttgg tacaggctaa cgacaatccc aggacatcca
    32581 cttcctgaaa tgcgtcggtg ccgataaacg gtgcggacac ctgaccagtg atggcaacga
    32641 cagggatgga atctaacagt gcgtccgcaa gcccggttat caggttggtt gcgcccggac
    32701 cagacgtggc gatacatacg ccagttttac cggtagcacg ggcataaccg atagccgcca
    32761 ttgccgcacc ctgctcatgt cggcacagca agtgctccac gccgccgtca tacaatgcat
    32821 cgtaaaccgg cataattgcg ccacccggat aaccgaaaac ggtgttcaca ccctgtgccc
    32881 gcaacgcatg taccacccac tgtgcgccat tcatagttag ttccccgtcc tgaatcttga
    32941 gaaacagaat tttgtgctgt tattcattgt ctgctcctcg gttatgtttt taaggtcaaa
    33001 aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat ctctaagcct
    33061 ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg ctaatcacga
    33121 ccaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct tgttcgaagg
    33181 aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg ggcctatttt
    33241 tatgatggct ttaataattt caat
//
LOCUS       DAZOYF010000028        33065 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZOYF010000028 DAZOYF010000000
VERSION     DAZOYF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.028"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(15..377)
                     /locus_tag="ACHUOF_001274"
     CDS             complement(15..377)
                     /locus_tag="ACHUOF_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR0610531.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(394..1299)
                     /gene="yhfZ"
                     /locus_tag="ACHUOF_001275"
     CDS             complement(394..1299)
                     /gene="yhfZ"
                     /locus_tag="ACHUOF_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR0610532.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(1569..2573)
                     /gene="trpS"
                     /locus_tag="ACHUOF_001276"
     CDS             complement(1569..2573)
                     /gene="trpS"
                     /locus_tag="ACHUOF_001276"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR0610533.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(2566..3324)
                     /gene="gph"
                     /locus_tag="ACHUOF_001277"
     CDS             complement(2566..3324)
                     /gene="gph"
                     /locus_tag="ACHUOF_001277"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR0610534.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(3317..3994)
                     /gene="rpe"
                     /locus_tag="ACHUOF_001278"
     CDS             complement(3317..3994)
                     /gene="rpe"
                     /locus_tag="ACHUOF_001278"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR0610535.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(4012..4848)
                     /gene="dam"
                     /locus_tag="ACHUOF_001279"
     CDS             complement(4012..4848)
                     /gene="dam"
                     /locus_tag="ACHUOF_001279"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0610536.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(4955..6247)
                     /gene="damX"
                     /locus_tag="ACHUOF_001280"
     CDS             complement(4955..6247)
                     /gene="damX"
                     /locus_tag="ACHUOF_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR0610537.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(6339..7427)
                     /gene="aroB"
                     /locus_tag="ACHUOF_001281"
     CDS             complement(6339..7427)
                     /gene="aroB"
                     /locus_tag="ACHUOF_001281"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR0610538.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(7484..8005)
                     /gene="aroK"
                     /locus_tag="ACHUOF_001282"
     CDS             complement(7484..8005)
                     /gene="aroK"
                     /locus_tag="ACHUOF_001282"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR0610539.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(8406..9644)
                     /gene="hofQ"
                     /locus_tag="ACHUOF_001283"
     CDS             complement(8406..9644)
                     /gene="hofQ"
                     /locus_tag="ACHUOF_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR0610540.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVMPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(9556..9962)
                     /gene="hofP"
                     /locus_tag="ACHUOF_001284"
                     /pseudo
     CDS             complement(9556..9962)
                     /gene="hofP"
                     /locus_tag="ACHUOF_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(9952..10392)
                     /gene="hofO"
                     /locus_tag="ACHUOF_001285"
     CDS             complement(9952..10392)
                     /gene="hofO"
                     /locus_tag="ACHUOF_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR0610541.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(10376..10915)
                     /gene="hofN"
                     /locus_tag="ACHUOF_001286"
     CDS             complement(10376..10915)
                     /gene="hofN"
                     /locus_tag="ACHUOF_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR0610542.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(10915..11694)
                     /gene="hofM"
                     /locus_tag="ACHUOF_001287"
     CDS             complement(10915..11694)
                     /gene="hofM"
                     /locus_tag="ACHUOF_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR0610543.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            11814..14366
                     /gene="mrcA"
                     /locus_tag="ACHUOF_001288"
     CDS             11814..14366
                     /gene="mrcA"
                     /locus_tag="ACHUOF_001288"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR0610544.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(14531..15091)
                     /gene="nudE"
                     /locus_tag="ACHUOF_001289"
     CDS             complement(14531..15091)
                     /gene="nudE"
                     /locus_tag="ACHUOF_001289"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR0610545.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            15411..17546
                     /gene="igaA"
                     /locus_tag="ACHUOF_001290"
     CDS             15411..17546
                     /gene="igaA"
                     /locus_tag="ACHUOF_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR0610546.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            17611..18279
                     /gene="yrfG"
                     /locus_tag="ACHUOF_001291"
     CDS             17611..18279
                     /gene="yrfG"
                     /locus_tag="ACHUOF_001291"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR0610547.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            18290..18691
                     /gene="hslR"
                     /locus_tag="ACHUOF_001292"
     CDS             18290..18691
                     /gene="hslR"
                     /locus_tag="ACHUOF_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR0610548.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            18716..19585
                     /gene="hslO"
                     /locus_tag="ACHUOF_001293"
     CDS             18716..19585
                     /gene="hslO"
                     /locus_tag="ACHUOF_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR0610549.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(19729..21487)
                     /locus_tag="ACHUOF_001294"
                     /pseudo
     CDS             complement(19729..21487)
                     /locus_tag="ACHUOF_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            21866..23488
                     /gene="pckA"
                     /locus_tag="ACHUOF_001295"
     CDS             21866..23488
                     /gene="pckA"
                     /locus_tag="ACHUOF_001295"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR0610550.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(23564..24916)
                     /gene="envZ"
                     /locus_tag="ACHUOF_001296"
     CDS             complement(23564..24916)
                     /gene="envZ"
                     /locus_tag="ACHUOF_001296"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR0610551.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(24913..25632)
                     /gene="ompR"
                     /locus_tag="ACHUOF_001297"
     CDS             complement(24913..25632)
                     /gene="ompR"
                     /locus_tag="ACHUOF_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR0610552.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            25860..26336
                     /gene="greB"
                     /locus_tag="ACHUOF_001298"
     CDS             25860..26336
                     /gene="greB"
                     /locus_tag="ACHUOF_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR0610553.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            26433..28754
                     /locus_tag="ACHUOF_001299"
     CDS             26433..28754
                     /locus_tag="ACHUOF_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR0610554.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            29192..29419
                     /gene="feoA"
                     /locus_tag="ACHUOF_001300"
     CDS             29192..29419
                     /gene="feoA"
                     /locus_tag="ACHUOF_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR0610555.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            29436..31757
                     /gene="feoB"
                     /locus_tag="ACHUOF_001301"
     CDS             29436..31757
                     /gene="feoB"
                     /locus_tag="ACHUOF_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR0610556.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            31757..31993
                     /gene="feoC"
                     /locus_tag="ACHUOF_001302"
     CDS             31757..31993
                     /gene="feoC"
                     /locus_tag="ACHUOF_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR0610557.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            32196..>33065
                     /gene="rpnA"
                     /locus_tag="ACHUOF_001303"
     CDS             32196..>33065
                     /gene="rpnA"
                     /locus_tag="ACHUOF_001303"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFR0610558.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAA"
BASE COUNT         7958 a         8963 c         8383 g         7761 t
ORIGIN      
        1 atttgtcggg agcttcaaac ctcttcgtta gcagccatcc ataatccata aagattggcc
       61 agaaaatagc cttcttcaca cgggttaact tccagagcga attccttcaa caactcctga
      121 tgcaattgca caacggcttg ccagtggctg gattgcgcca gttccgccag caactcgtta
      181 tccagcggct ctatttcttc accacggcga ctgcgcatca gtgcactcgc catatgtgtc
      241 atcgccatcg ttccttgctc actgcgcacc ggcagatggc actctttttc cagtacgttg
      301 acgacctgca tcatgccttt gcagacgtcc ttatcaataa cgcctgcctc gcaaagcagg
      361 ttgagtctgg tttccatatc agatgccttt cgtttaataa ctgggttcct gttccccact
      421 caccacccgt tgttgatggg caagcagggc gtgtttatct acaacagcac gcagcaattg
      481 ttgcattggg taatcatcga ctcgcgtcag gaccacggct tcggtggcct gtaaaaatcg
      541 cggatcgtct gttagcggcg tcgcctctaa tcccagcatg gtcagttcgt tttccgccac
      601 cacgttccag atcaccgcat cgacatcgcc tttaacaatg cgttgtaaac tctcgtgata
      661 agagagatcg actcgttcca catcactatc gccaaaaaag acatcggtca tgattttctg
      721 atccgccgaa cgactatcga gacctactcg cttaacattt ccagactcac ctttgcggca
      781 tatcagttga tgttcaccaa catacgtatg cgggccaagc tcgagggcga tgcacaaatt
      841 gttttggctg agataacttt ccgccgccag gcgggaaact actgccatgt catagacacc
      901 gttaagtaaa cactccacgc gaatatccgc gccgcgcata tgcgcatagt aaaaaggaat
      961 gccatcaaac tgggctttca atccgctcgc taaaccttcg tacaagcggg tatagggcaa
     1021 aggcatcgca cataccacgt tgttgatatc cacatgactc agcaatgctt tgttatccat
     1081 ctcgacaaga taactgccat tgcgcccacg gcgttcaatc cgtatcgctc cgcttgattc
     1141 cagcgttttc aacgcggcct gcgtcaggcc aaccgatgaa cggcattcag ttgccagctc
     1201 atctatggtt ttcagtcgat taccgcattt ttcacccaac aaataacggg ccagtgtcgt
     1261 gatgacgacg ccttcttttt tgataaacgt tcgacgcata atatattttc aataaaatga
     1321 atatttatat cttcattaaa atgaagataa gtggcgacga cgagatgtgg aaacggcgag
     1381 gcgcttcaca ttttttcggg ttgttgcgaa aagaggataa aaaaaccggg tttccccggt
     1441 ttcagagtga tgacgaagtg atttattcga caatgcgagc aaagggtaat tcccactggc
     1501 gcttgcctgc tcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgtccgagcg
     1561 ttttgatatt acggcttcgc cacaaaacca atcgcttcgt acaccgcttt cagcgtacgg
     1621 gaagcgtgta cactggcttt ttctgcgccg tctttcatca cctgttgcag gaaggcttca
     1681 tcgttgcgga aacggtgata gcgttcctgc aattcagtca gcataccgga aacggcatca
     1741 gccacttcac ctttcagatg accatacatc ttgccttcga actgtttttc cagttctggg
     1801 atgctctggc ccgttaccgc agaaaggata tccagcaggt tggaaacgcc cgctttgttc
     1861 tgcacatcgt agcgaactac cggcggctcg tcggagtcag tgaccgcgcg tttgattttc
     1921 ttcactaccg atttcggatc ttccagcagg ccgataacgt tattgcggtt atcgtcagac
     1981 ttggacatct tcttggtcgg ctccagcagc gacattacgc gcgcgccaga tttcggaata
     2041 aacggctccg gcaccttaaa gatctcgcca tacagcgcgt tgaaacgctg ggcgatatcg
     2101 cgactcagtt ccaggtgctg tttctggtct tcacccaccg gtaccagatt agtttgatac
     2161 agcaggatgt ccgcagccat cagcaccgga tagccaaaca gaccagcgtt gatgttctcg
     2221 gcataacgcg cagatttatc tttaaactgc gtcatgcgac tcagttcgcc gaagtaggta
     2281 tagcagttca gtgcccagcc taactgcgca tgttctggta cgtgggactg aacaaaaatg
     2341 gtgcttttct caggatcgat accacaagcc agatacaagg ccagcgtatc caacgtcgct
     2401 ttacgcagct tctgtgcatc ctggcgcacg gtgatcgcgt gttggtcaac gatacagtaa
     2461 atgcaatggt agtcatcctg catgtttacc cactgacgca gcgcacccat gtagttacca
     2521 atggtcaatt cacctgaggg ctgtgcgcca ctaaaaacga tgggcttagt catttttcga
     2581 ttcctgattt tcgctatgcg gaagccctaa tgcgggcaga aggtcattta tagactgata
     2641 aattacatca ggctggctga gatcgatagc ctcgccgtag ttgtatccgt aggttaagcc
     2701 aactgatggg caacctgccg cttttgccgc ctgaatatca ttccgtgagt cgccgacaaa
     2761 cagcatctgt tgtggggcaa ttcccatccg ctcagccacc agtaacagcg ggtccggatg
     2821 cggttttttg ttttgcacat catcgccgcc gataaccacg ctaaagtatt tggcaatatc
     2881 taaggcttcg agcagcggcg cgacgaacgg cgtcggtttg ttggtgacca agcctagcgg
     2941 caggccttta gcctgcaacg cgcccaacgt atcggcaacg tgcgggaaca aaaacgtccc
     3001 ctcttcggca acctcgccat agtagcgatc gaacagttta cgcagaatac gtacctgttc
     3061 ttctgccgga atgtcgtcat caacgggcgg tttccccatt gttttacgca gagtcgcacg
     3121 ttcctgacgc gcccaggtca atgcgcgctc catcagaaca tctgcgccgt taccaatcca
     3181 ggtaataacg cgttcttcac ctgcgacggg caactccagc gcatacagcg ccatatctac
     3241 cgcagcagca agaccaggag cactgtcgac cagcgtacca tcaagatcaa aagcgacgcc
     3301 gcgaatatct tcaaacttat tcatgactta cctttgccag ttcactgcgc atttcatcaa
     3361 tgactttttt atagtctggc tggtcgaaga ttgccgaacc ggcgacgaac atatccgcgc
     3421 ccgccgcagc gatttcgcca atgttgttca ccttcacgcc accgtccact tccagacgaa
     3481 tgtcaaagcc agactcgtcg atacggcgac gtacttcgcg cagtttatcc agtgtttgag
     3541 gaatgaaaga ctgaccgccg aaaccagggt tgacggacat cagcaggatc acatccagct
     3601 tatccatcac gtaatccaga tagctcagag gtgtcgccgg gttaaatacc agacccgctt
     3661 tacagccatt ttctttaatc agttgcagcg tgcggtcaac atgctcggag gcttctggat
     3721 gaaaggtaat gatgctggca ccagcggcag cgaaatcagg cacaatgcga tcgacgggtt
     3781 tcaccatcag atgtacgtcg ataggggcgg taatgccgta gttacgcaag gatttcagca
     3841 ccattggccc aatcgtcaga ttgggaacat agtggttatc catgacgtca aaatgcacga
     3901 catcagcgcc agctgccagg gcttttgcgg tatcttcacc caggcgggca aaatcagccg
     3961 acagaattga gggggcaatc aaatactgtt tcatccgctt ctccttgaga attatttttt
     4021 cgcgggtgaa acgactcctg gtttgtacaa agccagcagt tcgtccacct ttttacgtgt
     4081 gccgccgttg ctgcttatac tgcgtcgaac tttgacgaca tgcaattttg cgcgctgata
     4141 ccactcacgc gttaacatcg tatcgtgatt ggagatcagc actggaatat ggcgctcaac
     4201 cagaccttcg gcgatctccg ccagatgcgc ttgttgttca agcgtaaaac tgtttgtgtg
     4261 atacgccgta aagttggcgg tcgcagacag cggtgcataa ggcggatcgc aatagacgac
     4321 ggatgcatca tctgcgcgct ccatgctatc ggcgtaagac tcacaataga aaaaggcatt
     4381 ctgcgctttt tcagcgaagt gatacaactc tgcttccggg aaatagggtt ttttgtagcg
     4441 gccaaacggc acgttaaact caccgcgcag attgtaacga cacaggccgt tgtaaccgta
     4501 gcggttcaaa tataaaaaca gtaccgcccg acggaacgga tcctggcttt tgttgaactc
     4561 ttcgcggaac tgatagtaaa cctcggcgca atttgtttcg ggaacaaaca gctcgcgtgc
     4621 ggcctgtacg tactcatcag tacgcatctt cacaatgtta tagagactga tcaggtcgct
     4681 attgatatcg gcaaggatat aacgagaaaa gtcggtgttg agaaacaccg acccggcacc
     4741 tacaaaaggc tcaaccagac attcgccctt gggcaaatgc cgtttaatat catcaagcag
     4801 gggatacttg ccccctgccc acttcaaaaa agcgcgattt ttcttcatgc cgattaacta
     4861 attacacctt ctccggctgt ggagaaagct ccgacagcat ccagcgcttc aggcagctcc
     4921 cgcaacgata aaccattgcg ggagataacc ttgattactt cagatcggcc tgtacctgac
     4981 gcagcggttt cgcccacggg tttttggcct gaacatctgc tggcaatgta gataccgctt
     5041 ttttcgcctc ttctttcgaa gcgtacacgc cagaaaccag gacataccac ggctgaccat
     5101 tacgcgtcgt ttcatagaca acgtagtttt tcagattctc tttcttcgcc caaccgttca
     5161 ggttgtcgta gttagaggaa ctgctcagct gcagagtgta atggctggac ggtgccgatt
     5221 tcaacgaacc aacattacct gcggtcttcc ctccagcggc tggtgttgcc gtggtttgcg
     5281 ccggggatgc cgtctgtact ggagccgtag tcgccgtctc ttttggtgct ggcgtagaag
     5341 tcgcagccgg tgccttcgtg ctcgccacag gggcagctgg ttcaggttca gtacgcttcg
     5401 gcgtctgtgc taccggcttc ggctccgttt tcacggttgc ttgcggtttt ttcggttcaa
     5461 tcaccgcctg ctgacgaact gggcgcgtag tggacggacg ttcagcggtt tgcgttttcg
     5521 ccgtgtcacg cgatgcattg ccattgcgaa caggcgcaac cgttgcgggt tcagtcggca
     5581 acgtggaatt gaccgccaca ttgttcagct gttgctgatt ttgtggctgg gtcagcgcat
     5641 tgttcaggtc accctgcact tcaacacgtt gttgaccatc cgttgccacc ggggtttgcc
     5701 cttgagtcgg cgtagaagag atcggtggca gagaaacatc ctgctgagta ttttccgcag
     5761 acgtggttcc cggcgctggc tgcacgccat tcgcctgatc ggtcgcattg ccagcaagat
     5821 caatactctt ctcgccagac gcggtttgat cgctggaagt ggtcgagggg gcttttagcg
     5881 cagaaccgat accgatgatc aacagcagta gaaccagaat gccgacgccc atcatcatat
     5941 actgacgaga agcgggttta ctggctgctt ttttgcgctt acgcggacga cgctctacgc
     6001 gctcttcatc cacggtttca tcttcggatt cgtcaatttc ttcttcgatt tccggttcct
     6061 cattgcgctc tttttgcgca cgagtcggac ggcgatcgtc agtgtcatca agttcaatat
     6121 catcaaaatt gatctgcggt tcgccacgtt cagtacgctc agaacgttca gaagattgac
     6181 gagaacgacc agtacgacga tcgctgggat cgggtttcag ctcgtcttct ggtttgaatt
     6241 catccattta acaccccact aaaaggttaa tgcttaccac gttgcaatta acctgaagct
     6301 aatagaccgc ttgataagcg gcctgacctt tcttgttgtt acgctgattg acaatcggca
     6361 atggcgttaa gaacaagctc gtgcgaaacg ccgctgcgaa cttcactctt accaattgcc
     6421 aacggaagaa ttaagcgcat ctctcccgca aggactttct tgtcacgcag catatgcggt
     6481 aaatacgcct gcgcggacat ttcgcgcggc ccattgaccg gtaacccagc ccgcgtgagc
     6541 agggttataa tacgctgcgt ttcggcagaa ctaaactgcc cgagacgttc cgacgtccgc
     6601 gccgccatca ccatacccgc agcgaccgct tcaccatgta accaattgcc ataccccatt
     6661 tcagcttcaa tggcatgacc aaaggtgtgt cccagattca gtaaagcacg taacccggtt
     6721 tcgcgctcgt cggcggcgac aacttctgcc ttcagttcac aacaacggcg aatacagtac
     6781 gccattgccg gaccgtccag acgcaacaac gcatccagat tctcttccag ccagttaaaa
     6841 aacgcaccgt caagaataat gccgtatttg atgacttctg ccagccccga cgctaactca
     6901 cgcgggggaa gcgttttcag acagtcgaga tccaccacca ctgaagcagg ctggtagaac
     6961 gcgccaatca tgtttttacc gaggggatgg ttgaccgcag ttttgccgcc aacggaggaa
     7021 tcgacctgcg acagtaacgt cgtcgggact tgaatgaaac gaacaccgcg ctgataactc
     7081 gccgccgcga agccggtcag atcgcccact acgccgccgc caagcgccac cagcgtagta
     7141 tcgcgaccat gcggcttttg taacaacgcc gtaaagacgg tatcgagtac agctaggctt
     7201 ttatactgct cgccgtcagg gaggataacg ctatcgacgt taacacccgc ctgttcaagt
     7261 acgccgcgga ccttatcgag atacagagga gccagggttt cgctggtgac caacatgacc
     7321 tgctcgcccg atttcagcgg taagaatgaa gctggttcat taaacaaacc agatgcgatg
     7381 gtaattgggt aactacgttc cccgagagta acgacaatcc tctccataac gcgacatcca
     7441 ccttaattac tgtacccgca gacgagtgta tataaagcca gaattagttg ctttccagca
     7501 tgtgaataat ctggtttgca accactttag cgctttgatc atcagtacga atggtcacgt
     7561 cggcaatctc ttcatacagc ggattgcgtt cattggccaa cgcttccaga acttcacgcg
     7621 gcggtgtttc aacgtgcagc aacgggcgtt ttttatcacg ctgcgtgcgt gcaagttgct
     7681 tttcgatggt cgtttcaaga taaacgacaa cgccacgagc ggaaagacgg ttacgcgttt
     7741 cacgggattt cacagagccg ccgccagtag ccagcacaat accctgtttc tcggtcaact
     7801 cattgatgac cttttcttcg cgatcgcgga agccttcttc gccttctaaa tcgaaaaccc
     7861 agcccacatc agctccggtt cgtttctcaa tctcttgatc ggaatcgtaa aattccatat
     7921 tgagttgttg agctaactgg cgcccaatag tgctttttcc ggcacccata ggcccaacca
     7981 gaaagatatt gcgtttctct gccatttttt cggtactact aagactattc gttaatgata
     8041 aacccgcttc gctcagagag cgccgcagga catgaactga aacctcataa gatattgcga
     8101 gagtcagact gaaaattatc tcaatactcc agcgggtttg gcaactgaat aaatcaccca
     8161 tccctctgca tatctggtcg ctgcaagcgc gctgccttgc taccaccgct ctggcgataa
     8221 atcaccgggt aagattagcg taaaaaagac agcaaaatgc cgcctgaatg ataaatcatc
     8281 atcatggaac gcaggcagcg aagagcacca actctcaaat cggtactcct tgtatgctaa
     8341 atacctgcgc gcgtcaaata gatgaaacac gttcagcata aaaacattac cgcttttacg
     8401 gctgtttact cactggaaac cagtcgtggc gtgataaaca ccactaactc gcgtcgttca
     8461 tcttcttttc cgtcatgacg aaataattgc ccgaaccagg gaatgtcgcc aagcaacggt
     8521 acgctatcct gacccgattt atttttacgg gtaaaaatgc cgcccagcgc caacgtttct
     8581 ccgcttttta cctcgacctg cgtttcgatc tcctgcttat caatcgccag cacttcgcca
     8641 tcggcctgct gtagcacctg ccccggaacg ttctggctga tgtgtaattt cagccggatg
     8701 cgaccttttt gtaacaccgt gggcatgacc tccatcccca ggacggcctc tttaaattcc
     8761 accgacgtcg cgccactttc cccgctggaa acctgatatg gaatttcgct ctcctgttta
     8821 atgctggcag gctgaagatg tgaggccagc agacgcggac tggcgataat atccagctgc
     8881 tgtttttgtt cgagcgcgga aagctcaaga tccagcaagc gtccgttgat gcgcccaatg
     8941 ttaaaaccga catgcgttgt cgccgtcgct acggagaggt cgctaccaag cgtggtgact
     9001 tgcccaacgc caccagcgtg ttgcgcatcg gccagcgtcc atttcacgcc taactcacgc
     9061 aaactttttt cattaatggt gacaatatgc gccgacagct caacctgccc gaccggcaga
     9121 tccatttgcg ctacccactg ttcaagcgcg cttaacgccg ttttgttatc tcgtagcaaa
     9181 aggcgattgg tgcgtttatc gacggtcata ctccctttgg cactcagtag cttctccccc
     9241 gctttcgcca gttctcccgc gtcggcgtat tgcagggtta tactgcgatt ttccagcggc
     9301 agatttgcct gcgcccgcgc ctgctccgcc tcctggcggg cgatattgtc attctgccag
     9361 gcaatggaat gcactgagag aatgttgcct tcctgccgcg ttatcagtcc ggcgcttttc
     9421 actacagttt gtagtgcctg cttccaggga acatctgtta gatgtaacga caccgtaccg
     9481 ctgacgtctg gcgacacgac caggttcaac ttctcctgtt cagccagcgc ctgcaacacc
     9541 tgagctaccg gaacgtcatc caccatcagc gtcacttttt gcggctttgc cgcctgtacg
     9601 ccgggtatca gcatcaacag tagtgcggct atccattgct tcatttgtat ctccttgccg
     9661 ttgccacaac cattgtggcg gttcgcagtt tgtcccggta cccagcgtta gtacgtctgg
     9721 cgtcagctgt aaaattgtcc agccgttttc cagcacatcg ttttgctgca cccgtcgcca
     9781 tttcttttgc ccgtctttta ttacaccgat gatgcgctcg cctcgcccta ccatcccctg
     9841 atagcgccac tggctaagtt cgctaatccg gcatatagat cttccggcga tttaaaaggg
     9901 tcacgcatac cggttaaaag gcacaatgca atacctgcca acaaccagcg tttaaccctc
     9961 atgcggcatc tccagttgta gcgtgaacaa aagatcatca ccttccacgc ttaacgaaaa
    10021 acggctcacg ctgacgttgc gctctgccag ccgtgtaaat gccgacggca ctgcttccca
    10081 cagcgttttc aacgccaact cacctccctg cgcggatgga tgccaggaaa ccagttgcgc
    10141 gccggataac tgaaaatcca gtggcgaaaa gggcagcgtt ttttcctcgc tgaagggagt
    10201 ggtgtctacc aggcgataca gtgcggccca ctgctggtga tgacttgccc gtagtcgaat
    10261 taatgcgtcg cgctcttcat ggtgtgtcga tgatagaaaa atgagcgtaa ctaacatcag
    10321 caaccagaat gcccagcaaa actggcggag gcggggtgat gtggcgaacc accagtcaaa
    10381 gaacatgttc atcgctaacc ttccttgtta actgatactc aaattgccag cgtccctgcg
    10441 catcctgctg cgtggctccc cgctgattga gatgaaaaga cgcatcctgg cggagtgacg
    10501 tttctagtgc gtttaacgcg gtaatgcttg ttgtcagccc cttgatctcc agcgttccct
    10561 gctgccagct tatcgttgtc agccaggcgt gctcgggtaa aagcgccgcc agtgcttcca
    10621 gcgcagattg ccagtcgcgg gtaaattgtc gctggcgctg ccgttgcgaa cgctgttcgc
    10681 gtaactgctg ttgctccagc aaacgtggct tcgttatctg taagctgcgg gcgagttgtt
    10741 gttccgcctg aagcaaaact gcgtctatgc gcgcttcggc gctgcctgtc agacgcagta
    10801 ttagcgttat cccgacggcc agcagcagag gcgcaacgaa catcagcaac cagaaacgca
    10861 gaaaagcggt ccggcgttgc tgtcgccagg gcaaaaaatt aattggcgga ttcatcagta
    10921 cgccttccca agcgccagcc ccagcgcgat ggcaaagtct ccaccaggcg gcggtagcgg
    10981 cggctgacga acagaaacgg cctcccaggg gtcaaatccg ccttcgccac atatcgcgac
    11041 gctgtcggga tcaacggata acgctgccgc cagctccttc gcgctagtca tccccaccgc
    11101 cagtttgcgc ccccagctat agcgcgtcgc ccacagccac tgttcgttat cacgccaggc
    11161 cagacattgc tgatgagaag gtaaaaaagg caggaatcgc tgtaatgcac tggcatccgg
    11221 ggtgatcgca ctaacatgaa cacgcaacct ttctgccagc gtaagcagcg ttgccagctc
    11281 tttgctttgc gcggcagtca cgttataagc ggggctgagt gagtcttcgc tgtaatcgaa
    11341 gcgcagggag tccggatcca tatccagctc gcgggccatc gtccctgaca gccaggccgt
    11401 ttgctcccgc tcaccaaggg acatcgacgg gcgcggaaat gaccgctgta atgtgcgact
    11461 ggcgggaaac gccaacataa tgtgatgacg ctgcggcagt tcgcgactcc acggtaacaa
    11521 cgttttagcc agctgctgcg catcaacaat ccgcccatct ttgataatgt cgtgcgccag
    11581 cggcaaccgc caccagcgtt gcaaaaagca ttcttttgcg ccccgcacga tcgcaaccgc
    11641 tgccgcttct tgctgttgta aatgcaaacc aatttgccag atcttaaatg tcattgtgat
    11701 gatctcctta tcacccgtca ctctgacggg tatatcaatg cgtctggctt gcctttatac
    11761 taccgcgcgt ttgtttataa actgcccaaa tgaaactaaa tgggaaattt ccagtgaagt
    11821 tcgtaaagta ttttttgatc cttgcagtct gttgcattct gctgggagca ggctcgattt
    11881 atggcctata ccgctacatc gagccacaac tgccggatgt ggtgacatta aaagatgttc
    11941 gcctgcaaat tccgatgcag atttacagcg ccgatggcga gctgattgct caatacggtg
    12001 agaaacgtcg tattccggtt acgttggatc aaatcccacc ggagatggtg aaagccttta
    12061 tcgcgacgga agacagccgc ttctacgagc atcacggcgt tgacccggtg gggatcttcc
    12121 gtgcggcaag tgtggcgctg ttctccggcc acgcgtcaca aggggcgagt accattaccc
    12181 agcagctggc gagaaatttc ttcctcagtc cagaacgcac gatgatgcgt aagattaagg
    12241 aagtcttcct cgcgattcgc attgaacagt tgttgacgaa agacgaaatc ctcgagcttt
    12301 atctgaacaa gatttacctt ggttaccgcg cctatggtgt cggtgctgcg gcacaagtct
    12361 atttcggtaa aacggtcgac caactgacgc tgaacgaaat ggcggtgata gccgggttgc
    12421 cgaaagcgcc ttccaccttt aatccgctct actcaatgga tcgtgccgtc gcgcggcgta
    12481 acgtcgtact gtcgcggatg ctggatgaag ggtatatcac ccagcaacag ttcgatcaga
    12541 cacgcactga ggcgattaac gctaattatc acgcgccgga gattgctttc tctgcgccgt
    12601 acctgagcga aatggtgcgc caggagatgt ataaccgtta tggcgaaagt gcctatgaag
    12661 acggttatcg catttacacc accatcaccc gcaaagtgca gcaggccgcg cagcaggcgg
    12721 tacgtaataa cgtgctggac tacgacatgc gccacggcta tcgcggcccg gcaaatgtgc
    12781 tgtggaaagt gggcgagtcg gcgtgggata acaacaagat taccgatacg ctgaaggcgc
    12841 tgccgaccta tggtccgctg ctgcctgccg cagtcaccag cgccaatcct cagggagcga
    12901 cggcgatgct ggcggacggg tcgaccgtcg cattgagtat ggaaggcgtt cgctgggcgc
    12961 gtccttaccg ttcggatact cagcagggac cgacgccgcg taaagtgacc gatgttctgc
    13021 aaacgggtca gcaaatctgg gttcgtcagg ttggcgatgc atggtggctg gcacaagtgc
    13081 cggaagtgaa ctcggcgctg gtgtcgatca atccgcaaaa tggtgccgtt atggcgctgg
    13141 tcggtggctt tgatttcaat cagagcaagt ttaaccgcgc cacccaggca ctgcgtcagg
    13201 tgggttccaa catcaaaccg ttcctctaca ccgcggcgat ggataaaggt ctgacgctgg
    13261 caagtatgtt gaacgatgtg ccaatttctc gctgggatgc aggtgccggt tctgactggc
    13321 agccgaagaa ctcaccaccg cagtatgctg gtccaattcg cttacgtcag gggctgggtc
    13381 agtcgaaaaa cgtggtgatg gtacgtgcaa tgcgggcgat gggcgtcgac tacgctgcag
    13441 aatatctgca acgcttcggc ttcccggcgc aaaacattgt ccacaccgaa tcgctggcgc
    13501 tgggttcagc gtccttcacc ccaatgcagg tggcgcgcgg ctacgcggtc atggcgaacg
    13561 gcggcttcct agtggacccg tggtttatca gcaaaattga aaacgatcag ggcggcgtga
    13621 ttttcgaagc gaaaccgaaa gtagcctgcc cggaatgcga tattccagtg atttacggtg
    13681 atacgcagaa atcgaacgtg ctggaaaata acgatgttga agatgtcgct atctcccgcg
    13741 agcagcagaa tgtttctgta ccaatgccgc agctggagca ggcaaatcag gcgttagtgg
    13801 cgaagactgg cgcgcaggag tacgcaccgc acgtcatcaa cactccgctg gcattcctga
    13861 ttaagagtgc tttgaacacc aatatctttg gtgaaccagg ctggcagggt accggctggc
    13921 gtgcaggtcg tgatttgcag cgtcgcgata tcggcgggaa aaccggtacc actaacagtt
    13981 cgaaagatgc gtggttctcg ggttacggtc cgggcgttgt gacctcggtc tggattggct
    14041 ttgatgatca ccgtcgtaat ctcggtcata caacggcttc cggagcgatt aaagatcaga
    14101 tctcaggtta cgaaggcggt gccaagagtg cccagcctgc atgggacgct tatatgaaag
    14161 ccgttcttga aggtgtgccg gagcagccgc tgacgccgcc tccgggtatt gtgacggtga
    14221 atatcgatcg cagcaccggg cagttagcta atggtggcaa cagccgcgaa gagtatttca
    14281 tcgaaggtac gcagccgaca caacaggcgg tgcacgaggt gggaacgacc attatcgata
    14341 atggtgaggc acaggaattg ttctgattaa aaaggcgctt cggcgccttt tcagttggct
    14401 gacaaagtgc atttgtttat gccggatgcg ggtgaacgcg ttatccggct aacaaaatcg
    14461 tgaaaaatca atatattgca ggaaccccat aagcctgata agcgttgtgc atcaggcaaa
    14521 cttcacgcat ttacactcgc ccctgccctt tcaaccattc gcgcacgagg aacagcgcgc
    14581 tgacattacg cgcttcgttg aagtcagggt cttccagcaa atccatcata tgcgccagcg
    14641 gccagcgcac ctgtggtagc ggctctggct catcgccttc cagcgattcc gggtagagat
    14701 cttgcgcgac cacgatattc attttgctgg aaaagtaaga cggtgccatg ctgagcttct
    14761 tcaaaaaagt cagatcgttc gcaccaaatc caacctcttc ttttagctcg cggttagcgg
    14821 cttcgtagac gctttcaccc ggatcaatta atcctttcga aaaacctaat tcgtaggatt
    14881 cagttcccac tgcgtattcg cggatcagga tcaggtgatc gtccacaatc ggcacaatca
    14941 tcactgcttc ccggttggtt ggacgcattc gttcataaac acgccgcacg ccattgctga
    15001 actccagatc cacgctctcg acggtaaaca gtcgggaacg ggctacagtt tcaacattca
    15061 gaatggtggg tttttgtaat gatttgttca tcgtgggatc tatgctgtga aatcagcggt
    15121 tattgtgcga tatcggacac gctttcggca atgtgaattg catgttattt acatttatgt
    15181 aacttaataa ataatcgtcc tcaaatcaaa ttaaaagtca ataggttgaa ataactccag
    15241 gaatttgctg atattccgcc ttcagagggt ttgctatgat cagcggttac tgtgatgtgc
    15301 ttaatgatgc tcaagttaag ctccacgctt gccgatagcc aaccgcagaa tcatgtattg
    15361 tgtccggtgc gactgaccac gcctgacaga ctaagtaaga tggggaaagc atgagcacca
    15421 ttgtgatttt tttagctgct ttgctggcct gctcactact tgcgggatgg ctgataaaag
    15481 tgcgatccag acggcgtcag ctgccctgga ccaacgcctt cgcggatgcg caaacgcgta
    15541 aactcacacc tgaagaacgt agcgccgttg aaaattatct tgagagcctg acgcaggtat
    15601 tacaggtgcc tggcccaacg gaagctagcg cggcaccgat ctctctggcg ctgaatgccg
    15661 aaagcaacaa cgtcatgatg ctgacacacg ctatcacacg ttacggcatc tctaccgacg
    15721 atccgaataa atggcgttac tacctcgatt cggtagaagt ccacctgccc cccttctggg
    15781 aacagtacat caacgatgag aataccgttg aactgattca taccgattcg ctgccgttgg
    15841 ttatttcact caacggtcat acgctgcagg agtacatgca ggaaacgcgc ggctatgcct
    15901 tgcaacctgt tccgtccacg caggcgtcga ttcgcgggga agagagtgag caaatcgagc
    15961 tactcaatat tcgcaaagaa acgcatgaag aatatgcgct gagtcgtccg cgcgggctgc
    16021 gtgaagcgtt gctgatcgtc gcctccttcc tgatgttctt tttctgcctg attaccccgg
    16081 atgtatttgt tccgtggctg gcaggcggcg cattactgct gctgggcgca ggtctgtggg
    16141 ggctattcgc gcccccggca aaatcctccc tgcgagaaat tcattgtctg cgcggtacac
    16201 cccgtcgttg gggattgttt ggcgaaaacg atcaggaaca gatcaacaat atttcactcg
    16261 gtattatcga cctggtctat cccgcacact ggcagccata cattgctcag gatctcggtc
    16321 aacaaaccga tattgatatc tatctcgacc gccacgtagt gcgtcagggg cgatatcttt
    16381 cgctgcatga tgaagtaaaa aactttccgt tacagcactg gctgcgcagt acgattatcg
    16441 ctgcgggctc gctgctagtg ctgtttatgc tgttattctg gatcccgctg gatatgccgc
    16501 tgaaattcac tctctcatgg atgaaaggcg cgcagaccat tgaagccacc agcgtaaaac
    16561 aactggctga cgctggcgtg cgggtaggcg atacattgcg tattagcggt acgggaatgt
    16621 gtaatattcg cacttccgga acctggagcg cgaaaaccaa ttcacctttt ttaccgtttg
    16681 actgctcgca gatcatctgg aatgacgccc gctcattacc attaccagaa tctgaactgg
    16741 tcaacaaagc gacggcattg actgaagcag ttaatcgcca gctgcaccct aaaccggaag
    16801 atgaatctcg cgtcagtgcc tcattacgtt cagcgattca aaaatccggc atggtattgc
    16861 ttgatgattt tggcgacatt gtactgaaga cagcggattt atgttctgcc aaagatgact
    16921 gtgtgcgact gaaaaatgcg ctggtcaatc tcggcaacag taaagactgg gacgcgctgg
    16981 taaaacgcgc caacgccggg aagctcgatg gcgtgaatgt gttattacgc ccggtgagtg
    17041 ctgaatcgct ggataacctg gtggcaacct ccaccgcgcc gtttatcacg catgaaacgg
    17101 cgcgagcggc acaatcacta aacagtccgg cccccggcgg attcctgatt gtcagcgacg
    17161 aaggcagcga ttttgttgat cagccctggc cttcggcatc actttacgac tacccgccgc
    17221 aagaacagtg gaacgctttc cagaaactgg cacaaatgct gatgcatacg ccgtttaacg
    17281 ccgaaggtat cgtcacaaaa atcttcactg acgccaatgg tacgcagcat attggccttc
    17341 atccgatccc ggatcgttcc ggcctgtggc gctatctcag caccacattg ctgctactga
    17401 cgatgctggg tagcgccatt tacaatggcg tacaggcctg gcgtcgttac cagcgtcatc
    17461 gcactcgcat gatggagatt caggcctatt atgaaagctg cctgaacccg caactgatca
    17521 ccccttcaga aagccttatc gaataacacg tttgcgcggc aggttatgct accctgtcgc
    17581 gcaaattgct tcactctgga gatttccctc atgcatatca acattgcctg gcaggacgta
    17641 gataccgttc tgctggatat ggacggcacg ttgctcgacc tcgccttcga taactatttc
    17701 tggcaaaagc tggtgcctga aacatggggc gcgaaaaacg gggttacgcc acaggaagcg
    17761 atggaatata tgcgccagca atatcacgac gtacagcata cgctaaactg gtactgtctt
    17821 gattactgga gtgagcaact gggtctggat atctgtgcga tgaccaccga gatgggaccg
    17881 cgcgccgtac tgcgtgaaga taccattccg tttcttgagg cactgaaagc cagcggtaag
    17941 cagcgaattt tgctcaccaa tgctcatccg cacaacctgg cggtaaaact tgagcatacg
    18001 ggtctggacg cacaccttga tttattactt tccacccaca catttggtta tccgaaagag
    18061 gatcagcggt tatggcatgc ggtggccgaa gctacgggtc tgaaagctga aagaacgctg
    18121 tttattgatg acagcgaagc gattctcgat gctgccgcgc aatttggtat tcgttactgc
    18181 ctcggcgtga ctaatcctga ttccgggatt gccgagaaac agtatcaacg ccatccgtca
    18241 ctgaatgact accgccgcct gatcccctcg ctaatgtgaa ggagacgcca tgaaagagaa
    18301 acctgctgtt gaggttcgac tggataaatg gctatgggct gcccgttttt ataaaacccg
    18361 cgcgctggcc cgtgaaatga ttgaaggcgg taaggtgcat tacaacgggc agcgcagcaa
    18421 gccgagcaaa atcgtcgagc tgaatgccac gctcactctg cgccagggaa atgacgaacg
    18481 cacggtgatt gtaaaggcga ttactgaaca gcgtcgcccc gccagcgagg cagccttgct
    18541 gtatgaagag actgcggaaa gtgtagagaa acgcgaaaaa atggcgatgg cacgtaaact
    18601 taatgcctta accatgccgc acccggaccg acgcccggac aaaaaagagc gccgcgacct
    18661 gttacgattt aaacacggcg acagtgaata actgtcacct gcaagagaga tgattatgcc
    18721 gcaacatgac caattacatc gctatctgtt tgaaaatttt gccgttcgcg gcgaactggt
    18781 aaccgtttcg gaaaccctgc aacagatcct tgagaaccac gattatccgc agcccgttaa
    18841 aaacgtgctg gcagaactgc tggttgcgac cagcctgtta accgctacgc tgaagtttga
    18901 tggtgatatc accgtacagc tgcagggcga cggtccgatg aatctggcgg ttattaacgg
    18961 taacaataac cagcaaatgc gcggtgtggc gcgcgtgcag ggcgaaattc cagaaaatgc
    19021 cgacctgaaa acgctggtcg gcaatggtta cgtggtgatc accattaccc cgagcgaagg
    19081 cgaacgctat cagggcgtag ttggtctgga aggtgatacc ctggcggcct gcctagaaga
    19141 ttactttatg cgttctgaac agctgccgac gcgcctgttt attcgcaccg gcgacgtaga
    19201 cggcaaaccg gctgcaggcg gtatgttgtt gcaggtaatg cctgcgcaaa atacccaaca
    19261 ggacgacttt gaccacctgg cgacgctaac cgaaaccatc aaaaccgaag aactgctgac
    19321 cttaccggca aacgaagtgt tgtggcgttt gtatcacgaa gaagaggtga cggtttacga
    19381 tccgcaggat gtggagttca aatgcacctg ctcgcgtgaa cgttgcgccg atgcgctgaa
    19441 aacgctgcct gatgaagaag ttgatagcat cctggcggaa gatggcgaaa ttgacatgca
    19501 ttgtgattac tgcggtaacc actatctgtt caatgcgatg gatattgctg aaatccgcaa
    19561 caacgcgtct ccggcagatc cgtaagttca ttaatgagta tgtccggcag agagggctct
    19621 gccggatttt acgacttaat ggcctgatgt gctgcgctta ccagcgccta tgaaataaat
    19681 cgcaaattat tttggtcaga tacagcgtcg tatccgataa aagcgttgtc acttacttaa
    19741 gtttatttcc agcgtttcac catctaccgt cagatcgcgc cacgctttcg gcctcgtccc
    19801 cagttttcca tccttcgcag ctgtgagtaa tttttctttc gttatttcgt tcggaagtaa
    19861 actcatatca agcgcgtccc attcttttct gtctgagtca aagccataga caatgactct
    19921 gtcatcatta aaagcaaaca ggatccgctc cgcctgacca tcactattca gatcttgctc
    19981 gaccagtacg caggcgtctt tttcaataca ggtcatcacg ttatagcgat cctggattaa
    20041 ggccgaccag aatgtcgcat ccggtttaac agaaccaggg gcaattaaca cattttcggc
    20101 gaatactttt tccgatacct gttcctgaag atgttgctct ccatctaacg ccatcagcag
    20161 atcccgcgcg cgtttcgggt ctttcataaa cccggcatcg cttttcagcg actcaagcgc
    20221 cgcacgtcca tagcgaccgc tctgttcgag catgtagata gttacctggt ctggcgtgtt
    20281 tttgccgctc tgataacgcg ccatatgact gttcacgcta atacgcatac tgtccagcac
    20341 cggcgaatta agaagcacca gtatcaccaa caccaataac gaaaccgcaa ggttcacttt
    20401 gccctgaaga acaaggggat tttgcccatt acgccagacg atactgacaa aataccccag
    20461 cgaccacacc agtaacacca gctccgccag cgcgccctgc aagcggtcga cagtccagcc
    20521 gtactgagcg acccgcagcc ataaccccca ggtggcgacg aacacataca gcggagcaac
    20581 cagcaaagcg gttttaatca ggcaacgcaa cggccccgtc cagggaagtg acgctttttg
    20641 cggatcgcgg acaatagcca ttaagatcaa ttgcaaaaag gccagcgtta acagcaaccc
    20701 ggcggcggag atgtggcgag aaatcgcgct caggcccgta aacggcaggg tgatgataaa
    20761 catcagggtt agcaatgata ctaacggcag caaccccgtg gcgattagcg tgaacaactt
    20821 ttgaatagag tcgattaaac gtgactgtgt acgcgcgagg atcaccgcca gtgcggtaac
    20881 cagaccaaat gtaagataca taaaccagtc ggttgcaaaa aagagcgtat tgaaaaacgt
    20941 gatgccaaca agtttaaaca actcacccca cagtaaaagc accagccacg tcaggccatt
    21001 ggagagaaaa atcaccagta atataagtac gttatgccat actgactggt aaaagtagct
    21061 atagcggcta ctgccattgc ggatacgaag gctttgttgt atccacggta gcaacaacat
    21121 tgccatcaac agcagatagc agccaaaatc ccaaatcgcc ttttcagctc tccaggggtt
    21181 catgccatca gtttgccact tcagccaacc gctcatccct gtcgtggcaa taaacaccag
    21241 cgccagccat ccctgtcgtg gcaataaaca ccagcgccag ccatccccaa aggcgcttct
    21301 gtttaaaaga gatcacggag aagagtaaaa cggatgaaaa cgcgacggtc gcaggcacgc
    21361 cataaacaat ccaactgtga ttttttccgg caagccaggc gataagcagg tagcaaacca
    21421 gcccctgaag taatccggta gcaatcatcc cccagcgtgt tgccggtgaa agttcaacgt
    21481 tatccatgtt ggttatccag aatcaaaagg tgggttaatt atcgcatccg ggcagtagta
    21541 ttttgctttt ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat actttaccgg
    21601 ttgaatttgc atcaatttca ttcaggaatg cgattccact cacaatattc ccgccatata
    21661 aaccaagatt taaccttttg agaacatttt ccacacctaa aatgctattt ctgcgataat
    21721 agcaaccgtt tcgtgacagg aatcacggag ttttttgtca aatatgaatt tctccagata
    21781 cgtaaatcta tgagccttgt cgcggttaac acccccaaaa agactttact attcaggcaa
    21841 tacatattgg ctaaggagca gtgaaatgcg cgttaacaat ggtttgcccc cgcaagaact
    21901 cgaggcttat ggtatcagtg acgtacatga tatcgtttac aacccaagct acgacctgct
    21961 gtatcaggaa gagctcgatc cgagcctgac aggttatgag cgcggggtgt taactaatct
    22021 gggtgccgtt gccgtcgata ccgggatctt caccggtcgt tcaccaaaag ataagtatat
    22081 cgtccgtgac gataccactc gcgatacttt ctggtgggca gacaaaggca aaggtaagaa
    22141 cgacaacaaa cctctctctc cggaaacctg gcagcatctg aaaggcctgg tgaccaggca
    22201 gctttccggc aaacgtctgt tcgttgtcga cgctttctgt ggtgcgaacc cggatactcg
    22261 tctttccgtc cgtttcatca ccgaagtggc ctggcaggcg cattttgtca aaaacatgtt
    22321 tattcgcccg agcgatgaag agctggcagg tttcaaacca gactttatcg ttatgaacgg
    22381 cgcgaagtgc actaacccgc agtggaaaga acagggtctg aactccgaaa acttcgtggc
    22441 gtttaacctg accgagcgca tgcagctgat tggcggcacc tggtacggcg gcgaaatgaa
    22501 gaaagggatg ttctcgatga tgaactacct gctgccgctg aaaggtatcg cttctatgca
    22561 ctgctccgcc aacgttggcg agaaaggcga tgttgcggtg ttcttcggcc tttccggcac
    22621 cggtaaaacc accctttcca ccgacccgaa acgccgcctg attggcgatg acgaacacgg
    22681 ctgggacgat gacggcgtgt ttaatttcga aggcggctgc tacgcaaaaa ctatcaagct
    22741 gtcgaaagaa gcggaacctg aaatctacaa cgctatccgt cgtgatgcgc tgctggaaaa
    22801 cgtcaccgtg cgtgaagatg gcactatcga ctttgatgat ggttcaaaaa ccgagaacac
    22861 ccgcgtttct tatccaatct atcacatcga taacatcgtt aagccggttt ccaaagcggg
    22921 ccacgcgact aaggttatct tcctgactgc tgatgctttc ggcgtgttgc cgccggtttc
    22981 tcgcctgact gccgatcaaa cccagtatca cttcctctct ggcttcaccg ccaaactggc
    23041 cggtactgag cgtggcatca ccgaaccgac gccaaccttc tccgcttgct tcggcgcggc
    23101 gttcctgtcg ctgcacccga ctcagtacgc agaagtgctg gtgaaacgta tgcaggcggc
    23161 gggcgcgcag gcttatctgg ttaacactgg ctggaacggc actggcaaac gtatctcgat
    23221 taaagatacc cgcgccatta tcgacgccat cctcaacggt tcgctggata atgcagaaac
    23281 cttcactctg ccgatgttta acctggcgat cccaaccgaa ctgccgggcg tagacacgaa
    23341 gattctcgat ccgcgtaaca cctacgcttc tccggaacag tggcaggaaa aagccgaaac
    23401 cctggcgaaa ctgtttatcg acaacttcga taaatacacc gacacccctg cgggtgccgc
    23461 gctggtagcg gctggtccga aactgtaatg atttgaagct ggagaatatc tatccagtat
    23521 cttatagaaa gcaaaacggg aggcaccttc gcctcccgtt tatttaccct tcttttgtcg
    23581 tgccctgcgc ccgcgttacc ggcactggca gccaggcgcg aatggaaagc ccgccccgct
    23641 cgctggtgcc aagctccagc atcccgttat ggttatccac gatacgctgc acaattgcca
    23701 gccctaatcc cgtgccgcta atggtgcgcg cactgtcgcc gcggacaaac ggctggaaca
    23761 ggtgcttacg ttgttccggc gcaattcccg gaccgtcatc ttccacctgg aaccaggcgc
    23821 gattcggctc cgttccgctg ctgactttga tccagccatt gccataacgg gcggcgttga
    23881 ccaccatatt cgccaccgcg cgtttgatcg acagcgggtg cattttcact tcaatgctgc
    23941 cggggtaaag cgcggtttca atttcccgct catagccact ttcggcagca atcacctcac
    24001 cgagcactgc attaagatcc gccatttcca tcggcatctc ctgcccggtg cgcaggtagt
    24061 cgataaactg ctcaatgatg gcgttgcact cttcgatatc tttattgatc gattctgcca
    24121 gatagccatc ctgctcgctc atcatctcag tcgccaggcg aatacgcgtc agcggcgtgc
    24181 gcaagtcgtg acttaccccc gccatcagca gcgtgcggtc atccgccagt tgcttaacac
    24241 cagccgccat atggttaaag gcacgggtaa cggaacgcac ctccgaagcg ccatattcac
    24301 gcagcggcgg cggaataatc cctttaccaa cctgcaaggc tgcgtgttcg agatcgacca
    24361 acggtcggtt ctggatacga ataaacagcc acgccccgcc tatcgccaat agcataatcg
    24421 ccagcgtata gcggaacagc ggagagaaat cgccctgatg aatttcggtc agcggcacgc
    24481 gtacccagat attgggcgac agccaggttt tcagccagac gacaggcgaa cttttgttga
    24541 cctcaacgcg cacttccgtc gggccgccca gttgctgtgc catctgatgg cttaagaatt
    24601 catagtgttg cgcccaacgc agacctgcct cttcggcagc ctcgttggag tagagagaga
    24661 tccccagctc acggtagatc tcccgacgga aagcgggagg cacaaccaac tgcgtgccgt
    24721 cctccagttg cagtttgtcg gtcatcaaca tacgcacttc gtacgcgagg actttattaa
    24781 actgctggag gctcggcaaa atcgcgaagt tcagcaccac cagataagtc gtcaccaggc
    24841 tggcgaacag caaggtgacg atgagcaata acgtacgggc aaatgaactt cgtggcgaga
    24901 agcgcaatcg cctcatgctt tagagccgtc cggtacaaag acgtagccca ggccccaaac
    24961 ggtctgaatg taacgcggat gcgctggatc ttcttctacc atgcggcgca gacgcgaaat
    25021 ctgcacgtcg atggagcgtt ccattgcgga atattcacga ccacgggcaa ggttcatcag
    25081 cttatcgcgg gagagcggct cacgcggatg gctgaccagt gccttcagta ccgcaaactc
    25141 accgctggtg agcggcatcg gctcatcttc gcggaacatt tcgcgcgtac cgaggttaag
    25201 tttgaactta ccgaaagcaa ttaccgcctc ttcctgtgac ggtgcgcctg gcagttcgtt
    25261 cgcctgacga cgcagcaccg cacgaatacg ggccagcagt tcacgcgggt taaacggttt
    25321 tggaatgtag tcgtcagcgc caatctccag gcctacgata cggtccactt cttccccttt
    25381 cgccgtcacc ataatgatcg gcatcgggtt gctctgacta cgaagacgtc ggcaaatcga
    25441 caagccatct tcaccaggta acattaaatc cagtaccata agatggaaag attcacgagt
    25501 cagcaggcga tccatctgtt ctgcattagc gacgcttcga acctggaagc cttgttcggt
    25561 gagataacgt tccagcagcg cacgcaggcg catgtcgtca tcgaccacca gaatcttgta
    25621 gttctcttgc attgtttgta ctcccaaagg ttcgcaacaa tttgtaagcg tgtattctta
    25681 aaaaagctca cgttcgtcac cagctaaatc tggtatgaat ttcagcctaa attgttacaa
    25741 agcatattaa acagcagctt aagtatacaa tttattcggc gaaacattat tgattctgtt
    25801 gatatgatca cgttataccc aatgtgcgca ttatcaaaca gacaaaggga atcaacgaga
    25861 tgaaaacgcc cctggttacc cgggaagggt atgaaaaact caaacaagag cttaattatc
    25921 tctggcgtga agaacgcccg gaggtcacaa aaaaggtgac ctgggccgca agtctgggcg
    25981 accgcagcga aaatgctgac tatcagtata ataaaaagcg tctgcgtgaa atcgaccgtc
    26041 gcgtgcgcta tctcactaaa tgcctggaaa atctcaaaat cgtcgattac tcccctcagc
    26101 aggaaggcaa agtctttttt ggcgcgtggg tggagattga aaacgacgat ggcgtgactc
    26161 accgtttccg tattgtcggc tacgatgaaa tttttggccg taaagattac atctctatcg
    26221 attccccgat ggcccgcgca ttgctgaaaa aagaagtcgg cgatctggcg gtggtgaata
    26281 cccctgccgg ggaagcgaac tggtatgtta atgctatcga gtacgtgaaa ccgtaaggaa
    26341 gagtcttaac ctcctgccga tggctggcat ttttgccagc cagtccgtat aactatcccc
    26401 tgatttttga tccgaaaaga tgaactcaaa ccatgatgaa tgattcgttc tgccgcatta
    26461 ttgcgggtga aattcaggcg cgcccggaac aggttgacgc tgccgttcgc ctgcttgacg
    26521 aagggaatac cgtgccgttt atcgcacgtt atcgtaagga aatcaccggc ggtctggatg
    26581 acacgcagct gcgtaatctg gaaacgcgtc tgagctatct gcgcgagctg gaagagagac
    26641 gtcaggcgat cctcaaatcc atttccgagc aaggcaaact caccgatgat ctggcgaagg
    26701 ccatcaacgc caccctaagc aaaaccgaac tcgaagacct ctacctgccc tacaaaccta
    26761 aacgccgcac ccgcgggcaa atcgccattg aagcagggct tgagccgttg gctgacctgc
    26821 tgtggagcga tccgtcacac acgccagaag tcgccgctgc acaatatgtt gatgccgata
    26881 aaggcgtggc agataccaaa gccgcgctgg atggcgcgcg ctatatcctg atggaacggt
    26941 ttgccgaaga tgccacgctg ctggcgaaag tgcgtgatta tctgtggaag aacgcgcatt
    27001 tggtttctac ggtggtgagc ggtaaagaag aggaaggggc gaaattccgc gactatttcg
    27061 atcatcacga accgttgtcc acggtgcctt ctcaccgcgc gctggcgatg ttccgtgggc
    27121 gtaacgaagg cgtactccag ctttcgctga atgccgatcc gcagttcgat gagccgccca
    27181 aagagagcta ttgcgagcaa atcatcatgg atcaccttgg cctgcgcctg aacaatgccc
    27241 cggcggatag ctggcgcaaa agcgtagtga gctggacctg gcgcatcaag gtgctgatgc
    27301 atctggaaac cgaactgatg ggtaccgtgc gcgaacgcgc ggaagatgaa gcaatcaacg
    27361 tctttgcccg taacctgcac gatctgctga tggcggcccc tgccggactg cgtgcaacga
    27421 tgggcctcga tccgggtctg cgtaccgggg taaaagtggc ggtggtcgat gccactggca
    27481 aactggtggc gaccgatacc atttacccgc acaccggaca agccgcaaaa gcagcgatga
    27541 ccgttgctgc cttgtgtgaa aaacataacg ttgaactggt ggcgatcggc aacggtacag
    27601 cttcccgcga aactgaacgt ttctatctcg acgtgcagaa gcagttcccg aaagtgaccg
    27661 cacagaaagt gatcgtcagc gaagctggcg cgtcggttta ctcagcttcc gagctggcag
    27721 cgcaggagtt cccggatctc gacgtttcgc tgcgtggtgc ggtatctatc gcccgccgtt
    27781 tgcaggatcc gctggcggag ctggtgaaaa tcgatccgaa atctatcggc gtaggtcagt
    27841 atcagcatga cgtcagccag acgcaactgg cccgcaaact ggatgcagta gtagaagact
    27901 gcgtaaacgc cgttggcgtc gatctcaaca ccgcttctgt tccgctatta acccgcgtgg
    27961 cgggcctgac gcgcatgatg gcgcaaaaca tcgtggcctg gcgcgatgag aacggccagt
    28021 tccagaaccg tcagcaactg ctgaaagtca gccgtctggg gccgaaagcc ttcgagcagt
    28081 gcgcgggctt cctgcgcatt aaccacggtg ataacccgct ggacgcctct accgttcatc
    28141 cggaagccta tccggtggtg gaacgcattc tggcagcaac acagcaggca ctgaaagatc
    28201 tgatgggtaa cagcagcgaa ctgcgtaacc tgaaagcgtc tgactttact gatgaaaaat
    28261 tcggtgtgcc gacagtaact gacatcatca aagagctgga aaaaccgggt cgcgatccgc
    28321 gtccggaatt taaaaccgct cagtttgccg atggcgtcga gacaatgaac gacctgcaac
    28381 cgggtatgat cctcgaaggc gcagtgacca acgtcaccaa ctttggcgcg tttgtcgata
    28441 ttggcgtgca tcaggacggc ctggttcaca tctcttcatt gtcgaacaag tttgtggaag
    28501 atccgcatac cgtggtgaaa gcgggcgaca ttgtgaaggt gaaagtgctg gaagtggatc
    28561 ttcagcgtaa acgtatcgcc ctgactatgc gtctggacga gcagcctggc gaaaccaact
    28621 ctcgtcgcgg cggcggtaat gaacgcccgc aaaacaaccg cccggcagcc aaaccacgcg
    28681 gtcgtgaagc gcagcctgcc ggtaatagcg cgatgatgga tgcgctggcg gcagcgatgg
    28741 gtaaaaaacg ttaaacgccc ggactggccc ctaaacctgc gcgataaggc gttcacgccg
    28801 catctggcaa cgaacgcttt gcctgacata aaagtgccgg agaatatctc cggcactttt
    28861 attccacagc caaactcaca atatactccg ttaatattta tcacttcatt aacaactgaa
    28921 accttaatta aacattagcc agtccgggta actcactatt cgaattatat tttcgctgcg
    28981 atataacctt gagccacatc aacattgagt cagattatta ttcaaaccaa cattcgcaca
    29041 cattttaagt attgctgata gaaaccattc tcattatcat tgtgttgttg attatttaat
    29101 ctctcctttg ttggcaaatc atctggtctc atgtcgcggt caaacgcccc atgaggtagt
    29161 tatccagtta atgagaaaca agtaggcacc tatgcaatac actccagata ctgcgtggaa
    29221 aatcactggc ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc tttctctagg
    29281 catgttacct ggctcctctt ttgatgtggt gcgcgtcgct ccactcggcg accccattca
    29341 tatcgaaacc cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct tattagaagt
    29401 ggaagcggtt tcctgttaat acggtgataa caacaatgaa aaaattaacc attggcttaa
    29461 ttggtaatcc aaattctggc aagacaacct tatttaacca gctcactggc gcacgtcagc
    29521 gtgtaggtaa ctgggctggc gttaccgtcg aacgtaaaga agggcaattc tccaccaccg
    29581 atcatcaggt cacgctggtg gacctgcccg gcacctattc tctgaccacc atctcatcgc
    29641 agacctcgct cgatgagcaa atcgcctgtc actacatttt gagtggcgac gccgacctgc
    29701 tgattaacgt ggtggatgcg tctaaccttg agcgtaacct gtacctgacg ctgcaactgc
    29761 tggaactcgg cattccctgc atcgtggcgc tgaacatgct cgacattgcc gagaagcaaa
    29821 atattcgtat tgaaattgat gctctatcgg cacgtcttgg ctgcccggtg atcccgctgg
    29881 tttcaacccg tggtcgcggt attgaagcgc tcaagctggc gattgatcgc tataaagcta
    29941 acgagaatgt ggaactggtg cattacgcac agccgctact caacgaagca gattcactgg
    30001 caaaagtgat gccttccgac atcccgctga aacaacgtcg ctggctgggc ctgcaaatgc
    30061 tggaaggcga tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat ctggatgcca
    30121 ccctcgcccg tctgcgtaat gaaatggatg atccggcgct gcacattgcc gatgcgcgtt
    30181 accagtgcat tgctgccatc tgtgatgtgg taagcaacac cctgacggca gaacccagcc
    30241 gtttcaccac tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg ccgattttcc
    30301 tctttgtgat gtacctgatg ttcctgctgg ctatcaacat cggcggggcg ttacagccgc
    30361 tgtttaacgt cggctccgtg gcgctatttg tgcatggtat tcaatggatt ggctacacgc
    30421 tccacttccc ggactggctg actatcttcc tcgcccaggg cctgggtggc ggtattaaca
    30481 ccgtgctgcc actggtgccg cagatcggca tgatgtacct gttcctctcc ttccttgagg
    30541 actccggcta tatggcgcgt gcagcgtttg tgatggaccg cctgatgcag gcgctgggct
    30601 tgccggggaa atcctttgtg ccgctgatcg tcggtttcgg ttgtaacgta ccgtcggtaa
    30661 tgggcgcacg tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg atggcaccgt
    30721 ttatgtcctg cggcgcgcgt ctggctatct tcgcagtatt cgcggctgcc ttcttcgggc
    30781 agaacggtgc cctggcggtt ttctcgctgt atatgctcgg tatcgtgatg gcggtgctga
    30841 ctggcctgat gctcaagtac accatcatgc gcggtgaagc gacgccgttt gtcatggagc
    30901 tgccggtcta ccatgtacca cacgttaaaa gcctgattat ccagacctgg cagcgtctga
    30961 agggcttcgt tctgcgtgct ggtaaagtga ttatcatcgt cagcattttc ctgagcgctt
    31021 tcaacagctt ctcgttgagc gggaaaatcg tcgataacat caacgactcg gcgctggctt
    31081 ccgtcagccg ggtgataacc cctgtcttca agccgattgg cgtacatgaa gataactggc
    31141 aggcaacggt tggcctgttt acgggcgcaa tggcaaaaga agtggtggtg ggtacgctca
    31201 acaccctcta caccgcagaa aatattcagg acgaagagtt caatccggcg gaattcaacc
    31261 tcggtgaaga gctgttcagt gcggtagatg aaacctggca gagcctgaaa gacaccttca
    31321 gccttagcgt actgatgaac cccattgaag ctagcaaagg caacggtgaa atgggtaccg
    31381 gggcgatggg cgtgatggat cagaaattcg gtagcgcagc tgccgcatac agctacctga
    31441 ttttcgtcct gctgtatgta ccatgtatct cggtgatggg agccatcgcc cgtgaatcaa
    31501 gccgtggctg gatgggcttc tccatcctgt gggggctgaa tatcgcttac tcactggcaa
    31561 cattgttcta tcaggtcgcc agctacagtc agcacccaac ttacagcctg gtgtgcattc
    31621 tggcggtaat cctgtttaac atcgtagcta tcggtctgct gcgccgcgcg cgtagccggg
    31681 tggatatcga actgctggca acccgcaagt cggtaagcag ttgctgcgca gccagcacca
    31741 ccggtgattg ccattaatgg cttcacttat tcaggtacgt gatttgctgg cgttacgggg
    31801 ccgtatggaa gcgacccaga taagccagac attgaacact ccgcagccaa tgattaacgc
    31861 catgctgcaa cagctggaaa gcatgggcaa agccgtgcgg attcaggaag aacctgacgg
    31921 ctgcctctct ggcagttgta aaagctgccc ggaaggcaaa gcctgtctgc gcgagtggtg
    31981 ggcgctgcgt taaccatact ccatcgcctg ttttgaaaag cccggtatgc gtttgcatcc
    32041 gggctttttt gcgtgcggct ttccataaaa atgcaattct tgcagcgcaa cgtaagttcc
    32101 tctgaaagcg tctcgcaggg atgaaaactc gctaatgcac agatgtggag tggcgcgtag
    32161 agtcgcggca ttcaaacaac aggtgaagga acgccatgag taaaaagcag agttccaccc
    32221 cacatgatgc gctgttcaaa ctctttttac gccaaccgga gacggcacgc gattttcttg
    32281 cgtttcattt accggcaccc attcacgcgc tctgtgatat gaaaaccctc aagctggagt
    32341 cgaggagctt tattgatgac gatctgcgtg aaagctattc cgatgtgctg tggtcggtga
    32401 aaacggaaca aggaccagga tacatctatt gtctgattga acatcaaagc acctcaaaca
    32461 aactgatcgc atttcgcatg atgcgttacg ctattgccgc aatgcaaaat caccttgatg
    32521 ctggatacaa aacgttgccg atggtggtgc cattgttgtt ttaccacggt attgaaagcc
    32581 cctatcccta ttcgctgtgt tggctggatt gtttcgccga tcccaaactg gcaaggcagc
    32641 tttatgcctc cgcatttccg ctgattgatg tcaccgtcat gcctgatgat gaaatcatgc
    32701 agcaccgacg tatggcgctg ctggagttaa ttcaaaaaca tattcgtcaa cgcgatctga
    32761 tggggctggt agagcaaatg gcctgcttat taagtagtgg atacgctaat gacagacaaa
    32821 tcaaagggct gtttaattac atactgcaaa ctggcgacgc tgtacgtttt aacgatttta
    32881 tcgacggcgt tgccgaacgt tcaccgaaac acaaggagag tttaatgact attgcggaaa
    32941 gattgcggca ggagggggaa caatccaaag ccctgcatat agccaaaata atgcttgaat
    33001 ccggagtccc tcttgcagac atcatgcgat ttaccgggct gtcagaagaa gagttggctg
    33061 cggcg
//
LOCUS       DAZOYF010000029        31186 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZOYF010000029 DAZOYF010000000
VERSION     DAZOYF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.029"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            389..2047
                     /locus_tag="ACHUOF_001304"
     CDS             389..2047
                     /locus_tag="ACHUOF_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR0610559.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="ACHUOF_001305"
     CDS             complement(2044..3000)
                     /locus_tag="ACHUOF_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR0610560.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="ACHUOF_001306"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="ACHUOF_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR0610561.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="ACHUOF_001307"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="ACHUOF_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR0610562.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHUOF_001308"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHUOF_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR0610563.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6656..6730
                     /gene="yljB"
                     /locus_tag="ACHUOF_001309"
     CDS             6656..6730
                     /gene="yljB"
                     /locus_tag="ACHUOF_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR0610564.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="ACHUOF_001310"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="ACHUOF_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR0610565.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="ACHUOF_001311"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="ACHUOF_001311"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR0610566.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="ACHUOF_001312"
     tRNA            complement(9859..9946)
                     /locus_tag="ACHUOF_001312"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHUOF_001313"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHUOF_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR0610567.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHUOF_001314"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHUOF_001314"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR0610568.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHUOF_001315"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHUOF_001315"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR0610569.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHUOF_001316"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHUOF_001316"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR0610570.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHUOF_001317"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHUOF_001317"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR0610571.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="ACHUOF_001318"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="ACHUOF_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR0610572.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHUOF_001319"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHUOF_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR0610573.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="ACHUOF_001320"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="ACHUOF_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR0610574.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="ACHUOF_001321"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="ACHUOF_001321"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR0610575.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="ACHUOF_001322"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="ACHUOF_001322"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR0610576.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27412
                     /gene="dmsA"
                     /locus_tag="ACHUOF_001323"
                     /pseudo
     CDS             24969..27412
                     /gene="dmsA"
                     /locus_tag="ACHUOF_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27423..28040
                     /gene="dmsB"
                     /locus_tag="ACHUOF_001324"
     CDS             27423..28040
                     /gene="dmsB"
                     /locus_tag="ACHUOF_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR0610577.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28042..28833
                     /locus_tag="ACHUOF_001325"
     CDS             28042..28833
                     /locus_tag="ACHUOF_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009390 - dimethyl sulfoxide reductase
                     complex [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFR0610578.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACHUOF_001326"
     CDS             complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACHUOF_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR0610579.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29810..30958
                     /locus_tag="ACHUOF_001327"
     CDS             29810..30958
                     /locus_tag="ACHUOF_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0610580.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30976..>31186
                     /locus_tag="ACHUOF_001328"
     CDS             30976..>31186
                     /locus_tag="ACHUOF_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0610581.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQ"
BASE COUNT         7689 a         7644 c         8374 g         7479 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct
    25261 acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga
    25321 ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat
    25381 tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta
    25441 cggcaacgag tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg
    25501 ctcctggccg ccgggaaata ccctggtcgc gcgcctgatg aactgctgcg gcggttatct
    25561 taaccattac ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg
    25621 cggctgggca gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt
    25681 tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca
    25741 ggcacgccag aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg
    25801 tgccgggcgc gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa
    25861 cggtctggcg tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata
    25921 ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa
    25981 agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca
    26041 aatcaccggt gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa
    26101 accggtgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac
    26161 ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gagggaacag
    26221 cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc
    26281 gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat
    26341 gacggcgctg cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat
    26401 ctggaactat gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga
    26461 aatcctccag gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc
    26521 atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt
    26581 tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa
    26641 accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg
    26701 cgttgagcaa cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc
    26761 ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat
    26821 ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc
    26881 gcaggcaaac ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc
    26941 tgacattgcc gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta
    27001 cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac
    27061 aggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc
    27121 ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa
    27181 cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac
    27241 gccacgaatg atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc
    27301 aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct
    27361 cgctaagggg aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac
    27421 cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct
    27481 gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt
    27541 atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg
    27601 cctactatct gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc
    27661 cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca
    27721 ttggctgccg ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga
    27781 aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga
    27841 tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta
    27901 aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga
    27961 atattgtgat caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg
    28021 caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc
    28081 acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa
    28141 ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta
    28201 ctgatgggca ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt
    28261 aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc
    28321 ttttttgctg taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca
    28381 ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg
    28441 gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg
    28501 ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg
    28561 atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca
    28621 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
    28681 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc
    28741 gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg
    28801 catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg
    28861 gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca
    28921 ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc
    28981 agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc
    29041 cacgccgaat gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca
    29101 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
    29161 ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta
    29221 tcccaggcgt taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
    29281 agttctagaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt
    29341 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    29401 tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca
    29461 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    29521 gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga
    29581 gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca
    29641 ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata
    29701 taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg
    29761 gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta
    29821 tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt
    29881 gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg
    29941 cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt
    30001 cattaaacgc attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg
    30061 ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc
    30121 gggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac
    30181 gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt
    30241 tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt
    30301 tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca
    30361 ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct
    30421 tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc
    30481 tctgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact
    30541 ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg
    30601 actgctggtt ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag
    30661 ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc
    30721 ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca
    30781 agccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta gcgctatgct
    30841 aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta
    30901 tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat
    30961 gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg
    31021 acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt
    31081 gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc
    31141 cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcagg
//
LOCUS       DAZOYF010000030        30297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZOYF010000030 DAZOYF010000000
VERSION     DAZOYF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.030"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="ACHUOF_001329"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="ACHUOF_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HFR0610582.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="ACHUOF_001330"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="ACHUOF_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR0610583.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="ACHUOF_001331"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="ACHUOF_001331"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR0610584.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="ACHUOF_001332"
                     /pseudo
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="ACHUOF_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="ACHUOF_001333"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="ACHUOF_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR0610585.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNCIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="ACHUOF_001334"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="ACHUOF_001334"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR0610586.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="ACHUOF_001335"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="ACHUOF_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR0610587.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7490..7639
                     /locus_tag="ACHUOF_001336"
     CDS             7490..7639
                     /locus_tag="ACHUOF_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610588.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7840..9423
                     /locus_tag="ACHUOF_001337"
     CDS             7840..9423
                     /locus_tag="ACHUOF_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0610589.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="ACHUOF_001338"
     CDS             complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="ACHUOF_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR0610590.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="ACHUOF_001339"
     CDS             complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="ACHUOF_001339"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR0610591.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12423..13064)
                     /gene="udk"
                     /locus_tag="ACHUOF_001340"
     CDS             complement(12423..13064)
                     /gene="udk"
                     /locus_tag="ACHUOF_001340"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR0610592.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13382..13624
                     /locus_tag="ACHUOF_001341"
                     /pseudo
     CDS             13382..13624
                     /locus_tag="ACHUOF_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13623..14564
                     /gene="mdtA"
                     /locus_tag="ACHUOF_001342"
                     /pseudo
     CDS             13623..14564
                     /gene="mdtA"
                     /locus_tag="ACHUOF_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14564..17686
                     /gene="mdtB"
                     /locus_tag="ACHUOF_001343"
                     /pseudo
     CDS             14564..17686
                     /gene="mdtB"
                     /locus_tag="ACHUOF_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17687..20752
                     /gene="mdtC"
                     /locus_tag="ACHUOF_001344"
     CDS             17687..20752
                     /gene="mdtC"
                     /locus_tag="ACHUOF_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR0610593.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20753..22168
                     /locus_tag="ACHUOF_001345"
     CDS             20753..22168
                     /locus_tag="ACHUOF_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0610594.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22165..23568
                     /gene="baeS"
                     /locus_tag="ACHUOF_001346"
     CDS             22165..23568
                     /gene="baeS"
                     /locus_tag="ACHUOF_001346"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR0610595.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23565..24287
                     /gene="baeR"
                     /locus_tag="ACHUOF_001347"
     CDS             23565..24287
                     /gene="baeR"
                     /locus_tag="ACHUOF_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR0610596.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24478..24810
                     /locus_tag="ACHUOF_001348"
     CDS             24478..24810
                     /locus_tag="ACHUOF_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR0610597.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24957..26318
                     /gene="yegQ"
                     /locus_tag="ACHUOF_001349"
     CDS             24957..26318
                     /gene="yegQ"
                     /locus_tag="ACHUOF_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR0610598.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26660..26977)
                     /gene="yegR"
                     /locus_tag="ACHUOF_001350"
     CDS             complement(26660..26977)
                     /gene="yegR"
                     /locus_tag="ACHUOF_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR0610599.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27383..28282
                     /gene="yegS"
                     /locus_tag="ACHUOF_001351"
     CDS             27383..28282
                     /gene="yegS"
                     /locus_tag="ACHUOF_001351"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR0610600.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNSLIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28364..29137)
                     /locus_tag="ACHUOF_001352"
     CDS             complement(28364..29137)
                     /locus_tag="ACHUOF_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0610601.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29243..30283)
                     /gene="gatD"
                     /locus_tag="ACHUOF_001353"
     CDS             complement(29243..30283)
                     /gene="gatD"
                     /locus_tag="ACHUOF_001353"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0610602.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7012 a         8048 c         7827 g         7410 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 cacaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagcat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctagca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggagcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata cagttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgggtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgtgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7621 aggagtttct ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa
     7681 taatgtttta ttattaccgc ttttaattca gggataatgt gaggttatta ccctcaaata
     7741 atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt
     7801 gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg
     7861 tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac
     7921 ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg
     7981 gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctagcgtc aatctcctgg
     8041 ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac
     8101 ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa
     8161 cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc
     8221 gtggtgacgc aaattgtggt actggacgcc attttctcac tcgactcagt gattaccgcc
     8281 gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg
     8341 atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt
     8401 ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggcttc
     8461 gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcactc
     8521 aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaatcagac gctacgccag
     8581 cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac
     8641 gccaaaaccg cgtccatgct ggtggatcat ggaaaccagc agatctttaa tccgcaggaa
     8701 cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtaagcag cattatgacg
     8761 tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg
     8821 ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg
     8881 ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgcggcga accgctcaac
     8941 ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc
     9001 ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg
     9061 gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac
     9121 gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg
     9181 gcgaatggtc atatgccgct ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa
     9241 aaacgtgaat atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa
     9301 cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat
     9361 cgcgtgcaga aggtgcagat tataccgctg cgtaaggatg gcgagatgga gtacgaggtg
     9421 tgatggtatc ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc
     9481 gttgtctgtg gtaaacggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat
     9541 cgtttcctgt aggctggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga
     9601 tgttacctcg cgcccgtacc cgatagccga aaggaataag atgagggaac cgcgccagcg
     9661 gcgatttacc gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg
     9721 ttcaccggct tgagcgttcc atagtagcga ggaacatcaa gtgaacggaa cagccttcga
     9781 tcttacatat tcagaatata atagtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt
     9841 cttatcaggc ctacaggaca ggtcacctgg taacctacat cttctccagc aacttcttca
     9901 catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg
     9961 cttcgtcctg tagatgtttg cgcagtaact gatccacttg caggctgtaa ttgagttgct
    10021 gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc
    10081 tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg
    10141 ccagattcaa catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt
    10201 ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agccgcgtca
    10261 cgttatcgaa gttttcagcg gccttcacat caccgccatt acgctctacc gcttgctgaa
    10321 tcatctgctg gaagttcatc ccttccatac gtgtgtcggt catctcaaca tgcgcctgtc
    10381 cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca
    10441 gtgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtaccaatct
    10501 caacgttctc cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg
    10561 tgcccggcag tgaaaccttt ccaccgttaa gcttgccctg cagttgagta atttccagca
    10621 aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca
    10681 cgttactggc ctgcaacaaa atatcagctg taaagccttg cagtccctga taggccggtt
    10741 catcaatacg cgaagaaatg accgggcgcg gcaacgttga ttggctctgc ccctgctgcg
    10801 cggcaccgtt ttcaccattc gcggtttcat taagcgggat gaggttgtca agattcagtt
    10861 gcgggaattg cagtctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgtc
    10921 cgctcagcgt actgtcattg gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg
    10981 actcctgcca ctgggactgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg
    11041 caccctgcaa ctgccagtta atttgttcaa tagccgctgt taaatcatgc ggataatcag
    11101 aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc
    11161 cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc
    11221 ggattgtcac ctgctcgtca tcttcatgct ggaacaccag aacgctgtcc gccaccttaa
    11281 gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc
    11341 gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca
    11401 ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg
    11461 ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga
    11521 gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt
    11581 cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat
    11641 cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg accaagagta
    11701 tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat
    11761 cgattcggct ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt
    11821 aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg
    11881 gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc
    11941 agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccaaagagg
    12001 aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc agtgtcaccg
    12061 ccagcgccag ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga acgatctcat
    12121 cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa
    12181 acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg
    12241 cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc
    12301 aggcttcaat atctcggtca cacagacgca tggcattttc tcctttcgca catcactccc
    12361 ttaacgccaa ttacgtcaag ggcataccaa cttatcactg aacggtacac aatttatcaa
    12421 gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc
    12481 ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac
    12541 atcgggcgca cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg
    12601 ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg
    12661 gagaagttaa gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg
    12721 atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg
    12781 taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg
    12841 ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga
    12901 tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact
    12961 tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg
    13021 ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga
    13081 ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc
    13141 atccgtctga taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacaaaga
    13201 attcccggtg gcaaattacg ttgatcagtt ttatgtaagg taaaaaatgt tatacgcagt
    13261 tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact
    13321 tttgtactag cataaacaca gaaacgaata ctggcgacct ggccttgcgg ataaagcggt
    13381 aataagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt
    13441 cagtttaggt ttagtctcgt ttatctttac ccttgtctcg cttgagcttt cgcagtttgg
    13501 cacccaactc gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca
    13561 tgccgggcgc atgtggccgg gaattgcgct gagctgttcg ctgggaaata tcgccgcatc
    13621 cattccagga aggccagcag gtcaaagcag gcgatttact ggcagaaatt gaccccagcc
    13681 agttcaaagt tgcattagca caaacccagg gccaactggc aaaagataaa gccacgcttg
    13741 ctaacgcccg ccgtgatctg gcgcgttatc agcaactggc aaaaaccaat ctcgtctccc
    13801 gtcaggagct ggatgcccaa caggcgctgg tcagtgaaac cgaaggcacc attaaggctg
    13861 atgaagcaag cgtcgccagc gcgcagttgc aactcgactg gagccgcatc accgcaccag
    13921 tcgatggtcg cgttggtctc aagcaagttg atgttggtaa ccaaatctcc agtggtgata
    13981 ccaccggaat tgtggtgatc acccagacgc atcctatcga tttagtcttt accctgccgg
    14041 aaagcgatat cgctactgta gtgcaggcgc aaaaagccgg aaaaccgctg atggtagaag
    14101 cctgggatcg caccaattcg aagaaattaa gtgaaggcac gctgttaagc ctcgataacc
    14161 aaatcgatgc cacaaccggt acgattaaag tgaaagcacg ctttaataat caggatgatg
    14221 cgctgtttcc caatcagttt gttaacgcgc gcatgttagt cgacaccgaa caaaacgccg
    14281 tagtgatccc aacagccgcc ctgcaaatgg gcaatgaagg ccattttgtc tgggtgctga
    14341 atagcgaaaa caaggtcagc aaacatctgg tgacgccggg cattcaggac agtcagaaag
    14401 tggtgatccg tgcaggtatt tctgcgggcg atcgcgtggt gacagacggc attgatcgcc
    14461 tgaccgaagg ggcgaaagtg gaagtggtgg aagcccagag cgccaccact ccggaagaga
    14521 aagccaccag ccgcgaatac gcgaaaaaag gagcacgctc ctgatgcagg tgttaccccc
    14581 gagcagcaca ggcggcccgt cgcgcctgtt tattatgcgt cctgtcgcca ccacgctgct
    14641 gatggtggcg atcttactcg ccgggattat cggttatcgc gccctgcccg tttcggcgct
    14701 gccggaagtg gactatccga ccattcaggt ggtcacgctc tacccaggtg ccagcccgga
    14761 tgtcatgacc tctgccgtta ccgcgccgct cgaacgccag ttcgggcaga tgtctggcct
    14821 gaaacagatg tcgtcgcaaa gttccggcgg tgcgtcagtt atcactttgc agttccagct
    14881 aacattaccg ctcaatgtcg ccgagcagga agtgcaggcc gcgattaacg ctgcgaccaa
    14941 cttgttgccg agcgatctgc ctaacccgcc ggtttacagc aaagtgaacc cggcagatcc
    15001 gccgatcatg acgctcgccg tcacctcaac cgccatgccg atgacgcaag tggaagatat
    15061 ggtggaaacc cgcgtcgcgc agaaaatctc gcagatttcc ggcgtcggcc tggtgacgct
    15121 ttccggcggt cagcgtccgg ctgttcgcgt caaacttaac gctcaggcga ttgccgccct
    15181 cggcctgacc agcgaaaccg tgcgcaccgc cattaccggc gctaacgtta actcggcaaa
    15241 aggtagcctc gacggccctt cccgtgcggt cacgctttcc gcgaacgacc agatgcaatc
    15301 cgccgaagag tatcgccagc taatcatcgc ctaccagaac ggcgcgccaa ttcgtctggg
    15361 cgatgtcgca actgtagagc aaggtgcaga aaacagctgg ctcggcgcgt gggcgaacaa
    15421 agaacaggcc attgtgatga atgttcagcg ccagcccggt gctaacatta tctccaccgc
    15481 cgacagcatt cggcagatgc tgccacagct cactgagagt ctgccgaaat cggtgaaggt
    15541 gacagtgctt tccgatcgca ccaccaatat ccgcgcatcc gtcgatgata ctcagtttga
    15601 attgatgatg gctatcgcgc tggtagtcat gattatctac ctgtttttgc gcaatattcc
    15661 ggcgaccatc attcccggtg ttgctgtacc gctgtcgtta atcggcactt tcgcggttat
    15721 ggtgtttctc gatttttcaa tcaataacct gacactgatg gctttaacta tcgccaccgg
    15781 attcgtggtc gatgacgcca tcgtggtgat cgaaaacatt tcccgctata tcgaaaaagg
    15841 cgaaaaaccg ttggcggcgg cgctcaaggg cgcaggtgaa atcggcttta ccattatctc
    15901 gctgaccttc tcactgattg cggtgttgat cccactgctg tttatgggcg atatcgtcgg
    15961 gcgactgttc cgcgaatttg ctattaccct ggcggtagcg attttgatct cagcggtggt
    16021 gtcgctgacc ctgacaccga tgatgtgcgc gcggatgctc agccaggagt cgttgcgtaa
    16081 acagaacagc ttctcccgtg cctcggaaaa aatgttcgac aggataatcg ccgcctatgg
    16141 tcgtggactg gcgaaagtgc tgaatcatcc gtggctgacc ttaagcgtgg cactcagcac
    16201 gctgctgctt agcgtgctgc tgtgggtgtt cattccgaaa ggtttcttcc cggtacagga
    16261 caacggcatt attcagggca ctttgcaggc accgcaatcc agctcctttg ccaatatggc
    16321 ccagcgacaa cgccaggtcg cggacgtgat tttgcaggat ccggcagtgc aaagcctgac
    16381 ctcatttgtt ggcgttgatg gcactaaccc gtcgctgaat agtgcacgtt tacaaatcaa
    16441 cctcaaaccg ttggatgaac gtgatgatcg ggtgcaaaaa gtcatcgccc gtctgcaaac
    16501 ggcggtagat aaagtgccgg gcgtcgatct cttcctgcaa ccaacgcagg acctgaccat
    16561 cgatactcag gtcagccgca cccagtacca gtttaccttg caggccacgt cactggatgc
    16621 gctcagtacc tgggtgccac agttgatgga aaaactccag caactgccac agctttctga
    16681 tgtctccagc gactggcagg acaaagggct ggtggcgtat gtcaatgttg atcgcgacag
    16741 cgccagccgt ctggggatca gcatggcgga cgtcgataac gccctgtaca acgcgtttgg
    16801 tcagcgactg atttccacta tttatactca ggctaaccag tatcgcgtgg tgctggagca
    16861 caataccgaa aataccccag gcctcgcggc gctggatacc attcgcctga ccagcagcga
    16921 cggtggcgtg gtgccgttaa gctcaattgc caaaattgag cagcgttttg cgccgctttc
    16981 catcaaccat ctggatcagt tcccggtaac aaccatctcc tttaacgtgc cggataacta
    17041 ttcgctgggc gatgcggtgc aggcgattat ggacaccgaa aagacgctga atctgccggt
    17101 ggatatcacc acgcaattct agggcagcac cctcgccttc cagtcggcgc tgggcagcac
    17161 tgtctggctg attgtcgcgg cggtggtggc gatgtatatc gtgctcggca ttctgtacga
    17221 gagctttatt cacccgatca ccattctctc gacgctaccc accgcagggg tcggcgcgct
    17281 gctggcatta atgattgccg gtagcgaact ggatgtgatt gcgattatcg gcattatttt
    17341 gctgatcggt attgtgaaga agaacgccat catgatgatc gacttcgcgc tggctgctga
    17401 gcgcgagcaa ggcatgtcgc cgcgcgatgc aatctaccag gcttgtctgt tgcgttttcg
    17461 tccgatcctg atgaccaccc tggcggctct gcttggcgcg ctgccgctga tgttgagtac
    17521 cggggtcggc gcggaactgc gtcgtccgtt aggtatcggc atggtcggcg gtctgattgt
    17581 cagccaggtg ctgacgctgt tcaccacgcc ggtgatttat ctgctgttcg accgcctggc
    17641 attgtggacc aaaagtcgct ttgctcgcca tgaagaggag gcgtaagtga agttttttgc
    17701 cctcttcatt taccgcccgg tggcgacgat tttactgtcg gtcgccatta ccctgtgcgg
    17761 catactgggt ttccgtatgc tgccggtcgc cccgctgccg caggtcgatt ttccggtgat
    17821 tatggtcagc gcctcgctgc ccggtgcgtc gccggagaca atggcgtctt ccgttgccac
    17881 gccgctggag cgctcacttg ggcgcattgc cggggttagt gaaatgacct ccagcagttc
    17941 gctcggcagc acgcgcatta ttttgcagtt tgattttgac cgggatatca acggcgcagc
    18001 gcgtgatgtg caggcggcga tcaacgctgc acaaagtttg ctgcccagcg ggatgcccag
    18061 ccgcccgacc tatcgcaaag cgaacccgtc ggatgcgcca attatgatcc tcacgctgac
    18121 atccgatact tattcgcagg gtgaactgta cgatttcgcc tcgacgcagc tggctccgac
    18181 gatttcgcaa atcgacggtg tcggtgatgt cgatgtcggt ggcagctcgc tgcccgccgt
    18241 gcgcgtcggg ctgaccccgc aggcgctgtt taatcagggc gtgtcgctgg acgacgtacg
    18301 cactgccatc agcaatgcca acgtgcgtaa accgcagggg gcgctggaag atggcactca
    18361 ccgctggcag atccagacca acgatgaact gaaaaccgcc gctgaatatc agccgctgat
    18421 tattcactac aacaacggcg gcgcggttcg tctgggcgat gtggcgacgg tgactgactc
    18481 agtgcaggat gtgcgcaacg ccgggatgac caacgccaaa ccggctattt tactgatgat
    18541 ccgcaaactg ccggaagcca atattatcca gacggtagac agcatccggg caaaattacc
    18601 agagttgcag gagaccattc cggcggcgat tgatctgcaa attgcccagg atcgctcccc
    18661 caccattcgc gcctcgctgg aagaagtcga gcaaacgctg attatctcgg tggcgctggt
    18721 gattctggtg gtctttttat tcctgcgctc gggtcgcgcc actattattc ccgccgttgc
    18781 ggtgccggtt tcgctgattg gtacgtttgc ggcgatgtac ctgtgcggat tcagtctcaa
    18841 taatctttcg ctgatggcgc tcaccattgc tactggtttc gtggtggatg acgccattgt
    18901 ggtgctggaa aacattgccc gtcatctgga agcggggatg aaaccactac aggccgcgct
    18961 gcaaggtact cgcgaagtcg gctttacggt gctgtcgatg agtctgtcac tggtggcggt
    19021 gttcctgccg ctgctgttga tgggcggatt gccgggccga ctgttacgcg agtttgccgt
    19081 gacgctttct gtcgccattg gtatttcgtt gctggtttcc ctgacgctaa cgccaatgat
    19141 gtgcggctgg atgctaaaag ccagcaagcc gcgtgagcaa aagcgactgc gtggttttgg
    19201 ccgcatgttg gtagccctgc aacaaggcta cggcaagtcg ctgaaatggg tgctcaatca
    19261 tacccgtctg gtgggcgtgg tgctgcttgg caccattgcg ctgaatatct cgatcccgaa
    19321 aaccttcttc ccggagcagg acaccggcgt actgatgggt gggatacagg cggatcagag
    19381 tatttcgttt caggcgatgc gcggcaaatt gcaggatttc atgaaaatta tccgtgacga
    19441 tccggcagtg gataatgtca ccggctttac cggcggttca cgagttaaca gtgggatgat
    19501 gtttatcacc ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg
    19561 tctgcgcgtg aagctggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacaaga
    19621 tattcgcgtt ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga
    19681 cctggcggca ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga
    19741 actggcggac gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga
    19801 ccgcgacacc atggcacggc tgggaatcga cgtacaggcc gccaacagtc tgttaaataa
    19861 cgccttcggt cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt
    19921 gatggaagtg gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat
    19981 caataacgaa ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaatgc
    20041 cccactatcg gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc
    20101 gaccggaaaa tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg
    20161 tgtgccttcg acggtgcgcg gcagttttgc cggcacggcg caggttttcc aggagacgat
    20221 gaactcgcag gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat
    20281 cctttacgag agttacgtac atccgctgac gattctctcc accctgccct cggcgggcgt
    20341 tggagcgctg ttggcgctgg agctgttcaa tgccccgttc agcctaatcg ccctgatagg
    20401 gatcatgcta ttaatcggca tcgtgaagaa aaacgccatt atgatggtcg attttgcgct
    20461 tgaagcccaa cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct
    20521 gcgttttcgc ccgattatga tgaccaccct ggcggcgctg tttggcgcgc tgccgctggt
    20581 attgtcgggc ggtgacggct cggagctgcg gcaacccctg gagatcacca ttgtcggcgg
    20641 tctggtgatg agccagctcc tgacgctgta taccacgccg gtggtgtatc tctttttcga
    20701 ccgtctgcgg ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga
    20761 tcttcccgac agcacccgct ggcaattatg gattgtggct ttcggcttct ttatgcagtc
    20821 gctggacacc accatcgtaa acaccgccct tccctcaatg gcgcaaagcc tcggggaaag
    20881 tccgttgcat atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc
    20941 cgccagcggc tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt
    21001 gctgtttact ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct
    21061 ggcacgcgcg ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt
    21121 gatgaaaatc gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg
    21181 tcaggtcggt ccgctgctcg gtccggcgct cggcggtctg ctggtagaat acgcgtcgtg
    21241 gcactggatc tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgat
    21301 gttaatgccg aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct
    21361 ggcggttggc atggcggtat taaccctggc gctggacggc agtaaaggta caggtttatc
    21421 gccgctggcg attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct
    21481 gcacgccaga aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt
    21541 ttcgctgggc ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat
    21601 gacaccggtt ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat
    21661 gatcccgatg gtgcttggca gcatgggaat gaagcgaatt gtggtacagg tggtgaatcg
    21721 ctttggttat cgtcgggtac tggtagcgac cacgctgggt ctgtcgctgg tcaccctgtt
    21781 gtttatgact accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca
    21841 agggatggtc aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc
    21901 ggacaatctg gcgagcagcg gaaacagcct gctgtcgatg attatgcaat tgtcgatgag
    21961 tatcggcgtc accatcgccg ggctgttgct ggggcttttt ggttcgcagc atatcagcgt
    22021 cgacagcggc accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat
    22081 cgcccttccg gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat
    22141 ttcgcggcga aaaaggagcg cgcaatgaag ttctggcgac ccggtattac cggcaaactg
    22201 tttctggcga ttttcgccac ctgcattgtc ttgctgatca gtatgcactg ggcggtgcgt
    22261 atcagttttg agcgtggctt tattgattac atcaaacacg gcaatgaaca gcgattacag
    22321 ctgttaagtg atgcgcttgg cgagcagtat gcgcagcatg gtaactggcg cttcctgcgc
    22381 aacaacgatc gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat
    22441 aaacccggcc cgggtatgcc gccgcacggc tggcgtaccc agttctgggt ggtcgatcaa
    22501 aacaacaaag tactggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc
    22561 attctggtca acggtgcgga agtgggcgcg gtgattgcct cccctgttga gcggctgacc
    22621 cgcaatactg atatcaattt cgataaacaa cagcggcaaa ccagctggct gatcgtcgcc
    22681 ctggcaacgt tactcgcggc gctcgccacc tttctgctgg cgcgcggttt gctggcaccg
    22741 gtaaaacgac ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta
    22801 acgcccacca gtgaagatga actgggcaaa ctggcgcaag acttcaacca gctcgccagc
    22861 acgctggaga aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg
    22921 cgtacgccat tagcggtgct gcgcggtgaa ctggaagcca ttcaggatgg cgtgcgcaaa
    22981 ttcacgccgg agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt
    23041 gacgatcttc atcagttgtc gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg
    23101 gtagatttga tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc
    23161 cgtggcctga aactgcaatt ttccctgcca gacagtatca ccgtatttgg cgatcgcgac
    23221 cgtttaatgc agttattcaa taacttactg gaaaacagcc tgcgctacac tgacagcggc
    23281 ggcagcctga aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac
    23341 agtgcgccag gtgtcagtga cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc
    23401 gaaggttccc gcaaccgcgc cagcggcggt tccgggctgg ggctggcgat ttgcctgaac
    23461 attgttgaag cccataatgg ccgcattatt gccgcccatt cgccttttgg cggggtaagc
    23521 attacagtag agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa
    23581 tcgacgaaaa cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc
    23641 tcattgatta tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg
    23701 tactggcgta tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg
    23761 gcaccgatgg cctgacatta tgccgggaaa ttcgtcgttt ttccgatgtt ccgatcgtga
    23821 tggtgacggc aaaaatcgaa gagatcgacc gcctgctggg gctggagatt ggcgccgatg
    23881 actacatctg caagccgtac agcccacggg aagtggtagc gcgtgtcaaa accattttgc
    23941 gccgttgtaa accgcaacgc gaacttcagc aacaggatgc cgaaagcccg ttgattatcg
    24001 acgaaggtcg ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat
    24061 ttcgtctgct taaaacgctc tctcacgaac caggaaaagt gttctcccgc gagcaattgc
    24121 tcaatcatct ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta
    24181 aaaatctgcg ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt
    24241 acggcgttgg ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatt
    24301 actttgttag ccggagatgg cgttcagacc cgatccggca acattattcc acgcatggtc
    24361 agcaaactga cattatctct ccgccctggc aggcatcaga aaatcgctca tactttaatc
    24421 agtaaacagc acctttagat gctgttttcg ataagcaatt tcaatcaagg agtcattatg
    24481 gctggttggt ttgaactcag taagagcagt gataatcagt tccggtttgt gctaaaagcg
    24541 ggcaatggtg agactatcct caccagcgag ctgtatacct caaaagcctc tgcggaaaag
    24601 ggcatcgcgt cggtgcgtag caacagcccg caagaagagc gctatgagaa aaaaacggca
    24661 agcaacggca aattctattt caatctgaaa gccgctaatc atcaaattat cggctccagc
    24721 cagatgtacg ccaccgcgca atctcgtgaa accggaattg cctccgttaa agccaatggc
    24781 acaagccaga cggtgaaaga caatacgtaa ttacgatgcc gggcgtgggt tgttggcgtc
    24841 cggcgcttta ccagatcccc cctacagcgc tacaatgccc gcccttaaag tgggggcctc
    24901 ccctaaccgc ttcatcaggt gaagcggatc tgacctgtca tcagaacgag agaattatgt
    24961 ttaaaccgga actcctttcc ccggcgggaa cgctgaaaaa tatgcgttac gctttcgctt
    25021 atggcgcaga tgctgtttat gcgggccagc cgcgttactc cctacgtgtg cgcaacaacg
    25081 aattcaacca cgaaaatctt cagctcggca tcaatgaagc ccacgcgctg gggaaaaagt
    25141 tttatgtcgt ggtcaacatt gcaccgcaca acgccaagct gaaaaccttt atccgtgacc
    25201 tgaaaccggt ggtggaaatg gggccggatg cgctgattat gtccgatcca gggctgatta
    25261 tgctggtgcg tgagcacttc cctgaaatgc cgatccacct ctcggtgcag gctaacgccg
    25321 tgaactgggc gacggtgaaa ttctggcagc aaatgggact gacccgcgtg atcctctctc
    25381 gcgagctgtc gctggaagag attgaagaga tccataatca ggtgccggat atggagatcg
    25441 agatcttcgt tcacggcgca ctgtgcatgg cctactccgg tcgctgcctg ctctctggct
    25501 atatcaacaa gcgcgacccg aaccagggca cctgcaccaa cgcctgccgc tgggagtaca
    25561 acgtccagga agggaaagaa gatgacgttg gcaacatcgt acacaagtac gagccgattc
    25621 cggtgcaaaa tgttgagccg acgctgggta tcggcgcgcc aaccgacaaa gtgtttatga
    25681 tcgaagaagc ccagcgtccg ggcgagtata tgaccgcgtt tgaagatgag cacggcactt
    25741 acatcatgaa ctcgaaagat ctgcgcgcca tcgcccatgt agaacgcctg accaaaatgg
    25801 gcgtgcattc gctgaaaatc gaaggtcgta ccaaatcttt ctactattgc gcacgcaccg
    25861 cacaggttta ccgcaaagct atcgatgacg ccgctgcggg aaaaccgttc gataccagcc
    25921 tgctggaaac tctggaaggt ctggcgcatc gtggctatac cgaaggtttc ctgcgtcgtc
    25981 atactcacga cgattatcag aactacgaat acggttattc agtttctgac cgccagcagt
    26041 ttgttggtga gtttaccggt gagcgcaagg gggacctcgc agcggtagcg gtgaaaaata
    26101 aattctccgt tggcgacagt cttgagctga tgacgccgca aggcaacatt aactttaccc
    26161 ttgagcacat ggaaaacgcc aaaggcgaag ctatgccggt cgcaccaggc gatggttata
    26221 ctgtgtggct cccggtcccg caggatcttg agctaaatta cgcgctgcta atgcgtaatt
    26281 tctccgggga aaccacgcgt aacccccacg gtaagtgatt aatttcgatt atttttcccg
    26341 gatggaaaat tcttagaaac cgatcacata cagctgcatt tattaaggtt atcatccgtt
    26401 tcgctgaaaa acataatcca taaaatgcta gctgtaccag gaaccacctc cttagcctgt
    26461 gtaatctccc ttacacgggc ttattttttg tacataagct gtaaagaagg attgatccct
    26521 gataaccgtc ttccattgac cacatcgata gaatccccct tcatagcaca atgcctttca
    26581 cgtaacggta tcgtgctcgc ccaggttccg gctacgcaca gccagaacgc gcatgtttga
    26641 cgcttacaaa aaaatattct cactctccac atttgaatgt cagacgagcg acgccatgta
    26701 atcctgcacc ttctgtcttc aggtcaacta tctgcatttt tttgccctga gtaacacaga
    26761 aatgggctgc atcatttttt actatatttt ctgcaccaga tattctgccc ctggctaaag
    26821 aagcttcggc ttcggtgtag tattggttat cgagtttacg ctgaatatta cttttatatg
    26881 caagaccaaa tttaccgata cttgtttcat cattatgcac agcacaacca gatataagaa
    26941 aaatactaat taatgatata gcagctatct ttttcatctc accttccccc attaaatacc
    27001 aacgacactc tctagtgttt aaatataata atggcatgat tattataatt gaataggatt
    27061 ataataaatg ttctgtacaa catttcctac ataagtagga attacggaca ttgaggccct
    27121 tcagggtaac tccatggggg ctttaatata ttatattgaa gatgccactg tttagttgaa
    27181 tattaggtat atgctctttt ttgaaattta tcggtggcag ccgttagtat tcgctgtccc
    27241 cattgcaagc tcctggtggt aaccactgaa tcctccatac ttgaactgac tttttatcct
    27301 ccgactttca tcctgttctg actccacctt ttgttttctg ctctacacta tctacagacc
    27361 aatcataaag gcacatacga tcatggcaga atttcccgcc agcttactga ttcttaatgg
    27421 taaaagtact gacaatctac ccttgcgcga agcaattatg ctgttgcgtg aggaaggaat
    27481 gacgatccat gtgcgggtca cctgggagaa aggtgatgcc gcacgatttg tagaggaggc
    27541 ccggaagttg ggcgtcgcaa cggtgattgc cggtggtggc gatggcacca ttaatgaagt
    27601 ttctacggcg ttgattcagt gtgaggggga tgacataccc gcgctgggaa ttttgccatt
    27661 aggaaccgcc aatgattttg ccaccagtgt agggattcct gaggcactgg ataaggcgct
    27721 gaaactggca attgccggta atgccattgc gatagatatg gcgcaggtca acaaacaaac
    27781 ctgttttatt aatatggcga caggcggatt tgggacgcgt attaccacag aaacgccgga
    27841 aaaattaaaa gccgcgctgg gtggcgtctc ttacatcatt catggcttaa tgcgcatgga
    27901 tactctgcaa ccggaccgtt gtgaaatccg cggtgaaaac tttcactggc aaggtgacgc
    27961 cctggtcatt ggtattggta acgggcgtca ggccggtggc ggtcaacaat tgtgcccgaa
    28021 ctcgttaatt aacgatggct tgctgcaact gcgcattttt accggcgatg aaattcttcc
    28081 ggctctcgta tcaaccttaa aatctgacga agataacccg aatattatcg aaggcgcttc
    28141 gtcgtggttt gatatacaag ccccacacga aatcactttt aatcttgatg gcgaaccgtt
    28201 gagtgggcaa aacttccata ttgaaatact tccggcggcg ttgcgttgtc gattaccacc
    28261 agattgtcca ttattgcgtt aatcaaacta ttctctcttg aaccagggag aatccgtaat
    28321 gccggatgcg gtgtaaacac cttatccggc atacagaaca atactacacc gtaattaatt
    28381 ttacccgtgt cttctgcaat gccaatttat ccacctctga cacagcatca tcggtaacaa
    28441 taatgtcaat tttttcgatt ggcagtacct gattaaaacc gcgacgatta aatttcgatg
    28501 aatcgagaac ggcaatcact ttatttgcgg cggtgaccat cgccccgcta atcgaataac
    28561 cttcattaaa ggtcgtaata ccattaaccg catcaatacc atcagcaccg acaaacatta
    28621 aatcggcatt aatatcctgc aatgaacgct cggcaatgga accatgcatc gagcgcgttt
    28681 tatggcggac cgtgccacca cagacgacca gagtaatgtc tttattttcg gaaagggcaa
    28741 acgctgccgg gagactgttg gtaatcaccg tgatattttt agcggtcatt aatccttcgg
    28801 cgataagcat tgtggtactt ccgctgtcga gaataacggt catcccttca tggatcattt
    28861 tgactgccgc ctgcgctatt ctgttttttg gcgcgctggc gagctgaaat cgctctttaa
    28921 acccgacttc ctgggtctcg gtttcactat taccggacat tattttcgcc gcaccgccat
    28981 gaaagcgcgt aaccacgcct ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt
    29041 ccgaggcagc aaatactccc gccagatcct gaacaagcac ggttccctgt tcattcacta
    29101 attggatgat cttattcctt cgctcgaatg aattcatcgt catttaccca tattcaattg
    29161 tggctagtgt aaacgaaggg ggaccattgg tgaatgatct gttttgcaca ataccgggcc
    29221 agcatcacgc tggcccgcgg tttcatggaa tgagcaacac tttgccctgc atggcattac
    29281 gagcgatgtc acgcaccgcc tgggtgaagc tttcaaagct tccacggtga gcgattaatg
    29341 gctccaggct taacttacgt tctgtcagca accggctcgc cgtttcccac tcctgccccg
    29401 gccaagggct ggaatagttc atccaactgc cgataaccgt cagctcttta cgcaatattt
    29461 tgccaaacgt tgccgatgtt aaatgcagat cctgatgcaa cgtgcccacc agcgccagtt
    29521 gggcatgagg accggcaatc tctaccgcca gttcgacagt ttgcggtacg ccagccgtct
    29581 cgaggataag ctgattaaag cgcagttcgc gtaaaacgct ctgcatttgc ggcgcgctca
    29641 tttcgctact gttaaatgtt tgcatcgcac cgaaagattt tgccagtgcc agtttttctg
    29701 agctaatgtc gatcgccgtc acactctttg ctcccagcgc gacagcgcac tgaatagcca
    29761 gcaggccaat ggttccggca ccaataataa taacgttttt attctcacaa ccttgcgcta
    29821 aatgaaaagc atgcaggcca acggtaatcg gctcaataaa agccccatcc tcaataggca
    29881 tatccgtggg tagagcaaag acattttttc gcttaacgac aatatattca gcaaatccac
    29941 catcacgccg cgagccaata aaatcatatt ttgcgcactg ggaataaaac cctttcaaac
    30001 actctggaca agtaaaacag ggtaataacg gcacacaggc aaccgcatcg ccagggtgta
    30061 aatcatcaac accggatccc accgcatcaa tatagccgct aaattcatgg cctaacgtta
    30121 ttggataata atgtgcacca tttttaaata tcctgggtaa atcggaacca cataagcccg
    30181 agctggcaat ttttacccgc acctcatcct gatgtttaat ttcaggaatg acgctttttg
    30241 caacgcgcac gataccatca gtatcattca ctactgattt cataaaaact cctgatt
//
LOCUS       DAZOYF010000031        30288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZOYF010000031 DAZOYF010000000
VERSION     DAZOYF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.031"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..210
                     /gene="tuf"
                     /locus_tag="ACHUOF_001354"
     CDS             <1..210
                     /gene="tuf"
                     /locus_tag="ACHUOF_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR0610603.1"
                     /translation="GYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAM
                     DDGLRFAIREGGRTVGAGVVAKVLS"
     gene            440..823
                     /gene="secE"
                     /locus_tag="ACHUOF_001355"
     CDS             440..823
                     /gene="secE"
                     /locus_tag="ACHUOF_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR0610604.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            825..1370
                     /gene="nusG"
                     /locus_tag="ACHUOF_001356"
     CDS             825..1370
                     /gene="nusG"
                     /locus_tag="ACHUOF_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR0610605.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1529..1957
                     /gene="rplK"
                     /locus_tag="ACHUOF_001357"
     CDS             1529..1957
                     /gene="rplK"
                     /locus_tag="ACHUOF_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR0610606.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1961..2665
                     /gene="rplA"
                     /locus_tag="ACHUOF_001358"
     CDS             1961..2665
                     /gene="rplA"
                     /locus_tag="ACHUOF_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR0610607.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3078..3575
                     /gene="rplJ"
                     /locus_tag="ACHUOF_001359"
     CDS             3078..3575
                     /gene="rplJ"
                     /locus_tag="ACHUOF_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR0610608.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3642..4007
                     /gene="rplL"
                     /locus_tag="ACHUOF_001360"
     CDS             3642..4007
                     /gene="rplL"
                     /locus_tag="ACHUOF_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR0610609.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4327..8355
                     /gene="rpoB"
                     /locus_tag="ACHUOF_001361"
     CDS             4327..8355
                     /gene="rpoB"
                     /locus_tag="ACHUOF_001361"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR0610610.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8432..12655
                     /gene="rpoC"
                     /locus_tag="ACHUOF_001362"
     CDS             8432..12655
                     /gene="rpoC"
                     /locus_tag="ACHUOF_001362"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR0610611.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12897..14030)
                     /gene="thiH"
                     /locus_tag="ACHUOF_001363"
     CDS             complement(12897..14030)
                     /gene="thiH"
                     /locus_tag="ACHUOF_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR0610612.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14027..14797)
                     /gene="thiG"
                     /locus_tag="ACHUOF_001364"
     CDS             complement(14027..14797)
                     /gene="thiG"
                     /locus_tag="ACHUOF_001364"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR0610613.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14799..14999)
                     /gene="thiS"
                     /locus_tag="ACHUOF_001365"
     CDS             complement(14799..14999)
                     /gene="thiS"
                     /locus_tag="ACHUOF_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR0610614.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14983..15738)
                     /gene="thiF"
                     /locus_tag="ACHUOF_001366"
     CDS             complement(14983..15738)
                     /gene="thiF"
                     /locus_tag="ACHUOF_001366"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR0610615.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15731..16366)
                     /gene="thiE"
                     /locus_tag="ACHUOF_001367"
     CDS             complement(15731..16366)
                     /gene="thiE"
                     /locus_tag="ACHUOF_001367"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR0610616.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16366..18261)
                     /gene="thiC"
                     /locus_tag="ACHUOF_001368"
     CDS             complement(16366..18261)
                     /gene="thiC"
                     /locus_tag="ACHUOF_001368"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR0610617.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18342..18442)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18495..18971)
                     /gene="rsd"
                     /locus_tag="ACHUOF_001369"
     CDS             complement(18495..18971)
                     /gene="rsd"
                     /locus_tag="ACHUOF_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR0610618.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19066..19839
                     /gene="nudC"
                     /locus_tag="ACHUOF_001370"
     CDS             19066..19839
                     /gene="nudC"
                     /locus_tag="ACHUOF_001370"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR0610619.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19879..20943
                     /gene="hemE"
                     /locus_tag="ACHUOF_001371"
     CDS             19879..20943
                     /gene="hemE"
                     /locus_tag="ACHUOF_001371"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR0610620.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21100..21771
                     /gene="nfi"
                     /locus_tag="ACHUOF_001372"
     CDS             21100..21771
                     /gene="nfi"
                     /locus_tag="ACHUOF_001372"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR0610621.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21814..22404
                     /locus_tag="ACHUOF_001373"
     CDS             21814..22404
                     /locus_tag="ACHUOF_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR0610622.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22591..22863
                     /gene="hupA"
                     /locus_tag="ACHUOF_001374"
     CDS             22591..22863
                     /gene="hupA"
                     /locus_tag="ACHUOF_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR0610623.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22876..23571
                     /locus_tag="ACHUOF_001375"
     CDS             22876..23571
                     /locus_tag="ACHUOF_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR0610624.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23573..23998)
                     /gene="zraP"
                     /locus_tag="ACHUOF_001376"
     CDS             complement(23573..23998)
                     /gene="zraP"
                     /locus_tag="ACHUOF_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR0610625.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24236..25612
                     /gene="zraS"
                     /locus_tag="ACHUOF_001377"
     CDS             24236..25612
                     /gene="zraS"
                     /locus_tag="ACHUOF_001377"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR0610626.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25609..26934
                     /gene="zraR"
                     /locus_tag="ACHUOF_001378"
     CDS             25609..26934
                     /gene="zraR"
                     /locus_tag="ACHUOF_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR0610627.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26931..28220)
                     /gene="purD"
                     /locus_tag="ACHUOF_001379"
     CDS             complement(26931..28220)
                     /gene="purD"
                     /locus_tag="ACHUOF_001379"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR0610628.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28232..29821)
                     /gene="purH"
                     /locus_tag="ACHUOF_001380"
     CDS             complement(28232..29821)
                     /gene="purH"
                     /locus_tag="ACHUOF_001380"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR0610629.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7094 a         7896 c         8335 g         6963 t
ORIGIN      
        1 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctggtaccat cgaactgccg
       61 gaaggcgtag agatggtaat gccgggcgac aacatcaaaa tggttgttac cctgatccac
      121 ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg aaggcggccg taccgttggc
      181 gcgggcgttg tagcaaaagt tctgagctaa ttgccgataa catttgacgc aatgcgcact
      241 aaaagggcat catttgatgc cctttttgca cgctttcgta ccagaacctg gctcatcagt
      301 gattttcttt gtcataatca ttgctgagac aggctctgtt gagggcgtat aatccgaaaa
      361 gctaatacgc gtttcgattt ggtttgcctc gcgatcgcgg ggtgaaaatg tttgtagaaa
      421 acttctgaca ggttggttta tgagtgcgaa taccgaagct caaggaagcg ggcgcggcct
      481 ggaagcgatg aagtgggtcg ttgtggtggc attgctcctg gcggcgattg tcggcaacta
      541 cctttatcgc gacattatgc tgccgctgcg tgcgctggcc gtagtaattc tgattgctgc
      601 agcgggtggt gtcgcgctgt taacgacaaa aggtaaagct accattgctt ttgcccgtga
      661 agcgcgtacc gaagtccgta aggtcatttg gccgactcgc caggaaacat tgcacaccac
      721 gctgattgtg gctgcggtta ccgcagtaat gtcactgatc ctgtggggac tggatggtat
      781 tctggttcgc ctggtatcct ttatcactgg cctgaggttc tgagatgtct gaagctccta
      841 aaaagcgctg gtacgtcgtt caggcgtttt ccggttttga aggccgcgta gcaacgtcgc
      901 tgcgtgagca tatcaaatta cacaacatgg aagatttgtt tggtgaagtc atggtaccaa
      961 ccgaagaagt ggttgaaatc cgtggcggtc agcgtcgcaa aagcgaacgt aaattcttcc
     1021 ctggctacgt cctcgttcag atggtgatga acgacgcgag ctggcacttg gtgcgcagcg
     1081 taccgcgtgt gatgggcttc atcggcggta cttccgatcg tcctgcgcca atcagcgata
     1141 aagaagtcga tgcgattatg aaccgcctgc agcaggttgg tgataagccg cgtccgaaaa
     1201 cgctgtttga accgggtgaa atggtccgtg ttaatgatgg tccgttcgct gacttcaacg
     1261 gtgttgttga agaagtggat tacgagaaat ctcgtctgaa agtgtctgtt tctatcttcg
     1321 gtcgtgcgac cccggtagag ctggacttca gccaggttga aaaagcctaa cccagcgatc
     1381 aaaaaagcgg cgatttaatc gttgcacaag gcgtgagatt ggaatacaat ttcgcgcctt
     1441 ttgtttttat gggccttgcc cgtaaaacga ttttttatat cacggggagc ctctcagagg
     1501 cgttattacc caacttgagg aatttataat ggctaagaaa gtacaagcct atgtcaagct
     1561 gcaggttgca gctggtatgg ctaacccgag tccgccagta ggtccggctc tgggtcagca
     1621 gggcgtaaac atcatggaat tctgcaaagc gttcaacgca aaaactgatt ccatcgaaaa
     1681 aggtctgccg attccggtag taatcaccgt ttacgctgac cgttctttca ctttcgttac
     1741 caagaccccg ccggcagcag ttctgctgaa aaaagcggca ggtatcaagt ctggttccgg
     1801 taagccgaac aaagacaaag tgggtaaaat ttcccgcgct cagctgcagg aaatcgcgca
     1861 gaccaaagct gccgacatga ctggtgccga cattgaagcg atgactcgct ccatcgaagg
     1921 tactgcacgt tccatgggcc tggtagtgga ggactaagaa atggctaaac tgaccaagcg
     1981 catgcgtgtt atccgtgaga aagttgatgc aaccaaacag tacgacatca acgaagctat
     2041 cgcactgctg aaagagctgg cgactgctaa attcgtagaa agcgtggacg tagctgttaa
     2101 cctcggcatc gacgctcgta aatctgacca gaacgtacgt ggtgcaactg tactgccgca
     2161 cggtactggc cgttccgttc gcgtagccgt atttacccaa ggtgcaaacg ctgaagctgc
     2221 taaagctgca ggcgcagaac tggtaggtat ggaagatctg gctgaccaga tcaagaaagg
     2281 cgaaatgaac tttgacgttg ttattgcttc tccggatgca atgcgcgttg ttggccagct
     2341 gggccaggtt ctgggtccgc gtggcctgat gccaaacccg aaagtgggta ctgtaacacc
     2401 gaacgttgct gaagcggtta aaaacgctaa agctggccag gttcgttacc gtaacgacaa
     2461 aaacggcatc atccacacca ccatcggtaa agtggacttt gacgctgaca aactgaaaga
     2521 aaacctggaa gctctgctgg ttgcgctgaa aaaagcaaaa ccgactcagg cgaaaggcgt
     2581 gtacatcaag aaagttagca tctccaccac catgggtgca ggtgttgcag ttgaccaggc
     2641 tggcctgagc gcttctgtaa actaatgcct ttacgtgggc ggtgattttg tctacaatct
     2701 tacccccacg tataatgctt aatgcagacg tatatccgag atattcgggt tgtggcaagg
     2761 cggcaactga gtgagtcgcc aagagcatag ctaactatgt gactggtgcg aatgaaggaa
     2821 gccaacgccg ccacaagttg aatagcgacg gatagaaaag atttgttcgt tggagcctgg
     2881 cctatccagg cctccgtcga agaccgcagg agtttcgcaa gaaacttaat cccctgcgta
     2941 gacggtgaca gaacgctaag attattcttt tatattctgg cttgtttctg ctcaccgtaa
     3001 ttaagacgct ctctccgttt ggaggagtga agtgagttcc agagattttc tctggcaaac
     3061 atccaggagc aaagctaatg gctttaaatc ttcaagacaa acaagcgatt gttgctgaag
     3121 tcagcgaagt agccaaaggc gcgctgtctg cagtagttgc ggattcccgt ggcgtaactg
     3181 tagataaaat gactgaactg cgtaaagcag gtcgcgaagc tggcgtatac atgcgtgttg
     3241 ttcgtaacac cctgctgcgc cgtgctgttg aaggtactcc gttcgagtgc ctgaaagacg
     3301 cgtttgttgg tccgaccctg attgcatact ctatggaaca cccgggcgct gctgctcgtc
     3361 tgttcaaaga gttcgcgaaa gcgaatgcaa aatttgaggt caaagccgct gcctttgaag
     3421 gtgagctgat cccggcgtct cagatcgacc gcctggcaac tctgccgacc tacgaagaag
     3481 caattgcacg cctgatggca accatgaaag aagcttcggc tggcaaactg gttcgtactc
     3541 tggctgctgt acgcgatgcg aaagaagctg cttaatcgca gttatctttt taacgcattc
     3601 gcttacgtat aaacttattc tgatattcag gaacaattta aatgtctatc actaaagatc
     3661 aaatcattga agcagttgca gctatgtctg taatggacgt tgtagaactg atctctgcaa
     3721 tggaagaaaa attcggtgtt tccgctgctg ctgctgtagc tgtagctgct ggcccggttg
     3781 aagctgctga agaaaaaact gaattcgacg taattctgaa agctgctggc gctaacaaag
     3841 ttgctgttat caaagcagta cgtggcgcaa ctggcctggg tctgaaagaa gctaaagacc
     3901 tggtagaatc tgcaccggct gctctgaaag aaggcgtgag caaagacgac gcagaagcac
     3961 tgaaaaaagc tctggaagaa gctggcgctg aagttgaagt taaataagcc aacccttccg
     4021 gttgcagcct gagaaatcag gctgatggct ggtgactttt tagtcaccag cctttttgcg
     4081 ctgtaaggcg ccagtagcgt ttcacactgt ttgactactg ctgtgccttt caatgcttgt
     4141 ttctatcgac gacttaatat actgcgacag gacgtccgtt ctgtgtaaat cgcaatgaaa
     4201 tggtttaagc gtgatagcaa caggcattgc ggaaagtgtt ccattttccg gtcaacaaaa
     4261 tagtgttgca caaactgtcc gctcaatgga cagatgggtc gacttgtcag cgagctgagg
     4321 aaccctatgg tttactccta taccgagaaa aaacgtattc gtaaggattt tggtaaacgt
     4381 ccacaagttc tggatgtacc ttatctcctt tctatccagc ttgactcgtt tcagaaattt
     4441 atcgagcaag atcctgaagg gcagtatggt ctggaagctg ctttccgttc cgtattcccg
     4501 attcagagct acagcggtaa ttccgagctg caatacgtca gctaccgcct tggcgaaccg
     4561 gtgtttgacg tccaggaatg tcaaatccgt ggcgtgacct attccgcacc gctgcgcgtt
     4621 aaactgcgtc tggtgatcta tgagcgcgaa gcgccggaag gcaccgtaaa agacattaaa
     4681 gaacaagaag tctacatggg cgaaattccg ctcatgacag acaacggtac ctttgttatc
     4741 aacggtactg agcgtgttat cgtttcccag ctgcaccgta gtccgggcgt cttctttgac
     4801 tccgacaaag gtaaaaccca ctcttcgggt aaagtgctgt ataacgcgcg catcatccct
     4861 taccgtggtt cctggctgga cttcgaattc gatccgaagg acaacctgtt cgtacgtatc
     4921 gaccgtcgcc gtaaactgcc tgcgaccatc attctgcgcg ccctgaacta caccacagag
     4981 cagatcctcg acctgttctt tgaaaaagtt atctttgaaa tccgtgataa caagctgcag
     5041 atggaactgg tgccggaacg cctgcgtggt gaaaccgcat cttttgacat cgaagctaac
     5101 ggtaaagtgt acgtagaaaa aggccgccgt atcactgcgc gccacattcg ccagctggaa
     5161 aaagacgacg tcaaactgat tgaagtcccg gttgagtaca tcgcaggtaa agtggttgct
     5221 aaagactata ttgatgagtc taccggcgag ctgatctgcg cagcgaacat ggagctgagc
     5281 ctggatctgc tggctaagct gagccagtct ggtcacaagc gtatcgaaac gctgttcacc
     5341 aacgatctgg atcacggccc atatatctct gaaaccttac gtgtcgaccc aactaacgac
     5401 cgtctgagcg cactggtaga aatctaccgc atgatgcgcc ctggcgagcc gccgactcgt
     5461 gaagcagctg aaagcctgtt cgagaacctg ttcttctccg aagaccgtta tgacttgtct
     5521 gcggttggtc gtatgaagtt caaccgttct ctgctgcgcg aagaaatcga aggttccggt
     5581 attctgagca aagacgacat cattgatgtt atgaaaaagc tcatcgatat ccgtaacggt
     5641 aaaggcgaag tcgatgatat cgaccacctc ggcaaccgtc gtatccgttc cgttggcgaa
     5701 atggcggaaa accagttccg cgttggcctg gtacgtgtag agcgtgcggt gaaagagcgt
     5761 ctgtctctgg gcgatctgga taccctgatg cctcaggata tgatcaacgc caagccgatt
     5821 tccgcagcag tgaaagagtt cttcggttcc agccagctgt ctcagtttat ggaccagaac
     5881 aacccgctgt ctgagattac gcacaaacgt cgtatctccg cactcggccc aggcggtctg
     5941 acccgtgaac gtgcaggctt cgaagttcga gacgtacacc cgactcacta cggtcgcgta
     6001 tgtccaatcg aaacccctga aggtccgaac atcggtctga tcaactctct gtccgtgtac
     6061 gcacagacta acgaatacgg cttccttgag actccgtatc gtaaagtgac cgacggtgtt
     6121 gtaactgacg aaattcacta cctgtctgct atcgaagaag gcaactacgt tatcgcccag
     6181 gcgaactcca acctggatga agaaggccac ttcgtagaag acctggtaac ttgccgtagc
     6241 aaaggcgaat ccagcttgtt cagccgcgac caggttgact acatggacgt atccacccag
     6301 caggtggtat ccgtcggtgc gtccctgatc ccgttcctgg aacacgatga cgccaaccgt
     6361 gcattgatgg gtgcgaatat gcaacgtcag gccgttccga ctctgcgcgc tgataagccg
     6421 ctggttggta ctggtatgga acgtgctgtt gccgttgact ccggtgtaac tgcggtagct
     6481 aaacgtggtg gtgtcgttca gtacgtggat gcttcccgta tcgttatcaa agttaacgaa
     6541 gacgagatgt atccgggtga agcaggtatc gacatctaca acctgaccaa atacacccgt
     6601 tctaaccaga acacctgtat caaccagatg ccgtgtgtgt ctctgggtga accggttgaa
     6661 cgtggcgacg tgctggcaga cggtccgtcc accgacctcg gtgaactggc gcttggtcag
     6721 aacatgcgcg tagcgttcat gccgtggaat ggttacaact tcgaagactc catcctcgta
     6781 tccgagcgtg ttgttcagga agaccgtttc accaccatcc acattcagga actggcgtgt
     6841 gtgtcccgtg acaccaagct gggtccggaa gagatcaccg ctgacatccc gaacgtgggt
     6901 gaagctgcgc tctccaaact ggatgaatcc ggtatcgttt acattggtgc ggaagtgacc
     6961 ggtggcgaca ttctggttgg taaggtaacg ccgaaaggtg aaactcagct gaccccagaa
     7021 gaaaaactgc tgcgtgcgat cttcggtgag aaagcctctg acgttaaaga ctcttctctg
     7081 cgcgtaccaa acggtgtatc cggtacggtt atcgacgttc aggtctttac tcgcgatggc
     7141 gtagaaaaag acaaacgtgc gctggaaatc gaagaaatgc agctcaaaca ggcgaagaaa
     7201 gacctgtctg aagaactgca gatcctcgaa gcgggtctgt tcagccgtat ccgtgctgtg
     7261 ctggtagccg gtggcgttga agctgagaag ctcgacaaac tgccgcgcga tcgctggctg
     7321 gagctgggcc tgacagacga agagaaacaa aatcagctgg aacagctggc tgagcagtat
     7381 gacgaactga aacacgagtt cgagaagaaa ctcgaagcga aacgccgcaa aatcacccag
     7441 ggcgacgatc tggcaccggg cgtgctgaag attgttaagg tatatctggc ggttaaacgc
     7501 cgtatccagc ctggtgacaa gatggcaggt cgtcacggta acaagggtgt aatttctaag
     7561 atcaacccga tcgaagatat gccttacgat gaaaacggta cgccggtaga catcgtactg
     7621 aacccgctgg gcgtaccgtc tcgtatgaac atcggtcaga tcctcgaaac ccacctgggt
     7681 atggctgcga aaggtatcgg cgacaagatc aacgccatgc tgaaacagca gcaagaagtc
     7741 gcgaaactgc gcgaattcat ccagcgtgcg tacgatctgg gcgctgacgt tcgtcagaaa
     7801 gttgacctga gtaccttcag cgatgaagaa gttatgcgtc tggctgaaaa cctgcgcaaa
     7861 ggtatgccaa tcgcaacgcc ggtgttcgac ggtgcgaaag aagcagaaat taaagagctg
     7921 ctgaaacttg gcgacctgcc gacttccggt cagatccgcc tgtacgatgg tcgcactggt
     7981 gaacagttcg agcgtccggt aaccgttggt tacatgtaca tgctgaaact gaaccacctg
     8041 gtcgacgaca agatgcacgc gcgttccacc ggttcttaca gcctggttac tcagcagccg
     8101 ctgggtggta aggcacagtt cggtggtcag cgtttcgggg agatggaagt gtgggcgctg
     8161 gaagcatacg gcgcagcata caccctgcag gaaatgctca ctgttaagtc tgatgacgtg
     8221 aacggtcgta ccaagatgta taaaaacatt gtggacggca accatcagat ggagccgggc
     8281 atgccagaat ccttcaacgt attgttgaaa gagattcgtt cgctgggtat caacatcgaa
     8341 ctggaagacg agtaattctc gctcaaacag gtcactgctg tcggggtaaa acccggcagc
     8401 ggattgtgct aactccgacg ggagcaaatc cgtgaaagat ttattaaagt ttctgaaagc
     8461 gcagactaaa accgaagagt ttgatgcgat caaaattgct ctggcttcgc cagacatgat
     8521 ccgttcatgg tctttcggtg aagttaaaaa gccggaaacc atcaactacc gtacgttcaa
     8581 accagaacgt gacggccttt tctgcgcccg tatctttggg ccggtaaaag attacgagtg
     8641 cctgtgcggt aagtacaagc gcctgaaaca ccgtggcgtc atctgtgaga agtgcggcgt
     8701 tgaagtgacc cagactaaag tacgccgtga gcgtatgggc cacatcgaac tggcttcccc
     8761 gactgcgcac atctggttcc tgaaatcgct gccgtcccgt atcggtctgc tgctcgatat
     8821 gccgctgcgc gatatcgaac gcgtactgta ctttgaatcc tatgtggtta tcgaaggcgg
     8881 tatgaccaac ctggaacgtc agcagatcct gactgaagag cagtatctgg acgcgctgga
     8941 agagttcggt gacgaattcg acgcgaagat gggtgcggaa gcaatccagg ctctgctgaa
     9001 gagcatggat ctggagcaag agtgcgaaca gctgcgtgaa gagctgaacg aaaccaactc
     9061 cgaaaccaag cgtaaaaagc tgaccaagcg tatcaaactg ctggaagcgt tcgttcagtc
     9121 tggtaacaaa ccagagtgga tgatcctgac cgttctgccg gtactgccgc cagatctgcg
     9181 tccgctggtt ccgctggatg gtggtcgttt cgcgacttct gacctgaacg atctgtatcg
     9241 tcgcgtcatt aaccgtaaca accgtctgaa acgtctgctg gatctggctg cgccggacat
     9301 catcgtacgt aacgaaaaac gtatgctgca ggaagcggta gacgccctgc tggataacgg
     9361 tcgtcgcggt cgtgcgatca ccggttctaa caagcgtcct ctgaaatctt tggccgacat
     9421 gatcaaaggt aaacagggtc gtttccgtca gaacctgctc ggtaagcgtg ttgactactc
     9481 cggtcgttct gtaatcaccg taggtccata cctgcgtctg catcagtgcg gtctgccgaa
     9541 gaaaatggcg ctggagctgt tcaaaccgtt catctacggc aagctggaac tgcgtggtct
     9601 tgctaccacc attaaagctg cgaagaaaat ggttgagcgc gaagaagctg tcgtttggga
     9661 tatcctggac gaagttatcc gcgaacaccc ggtactgctg aaccgtgcac cgactctgca
     9721 ccgtctgggt atccaggcat ttgaaccggt actgatcgaa ggtaaagcta tccagctgca
     9781 cccgctggtt tgtgcggcat ataacgccga cttcgatggt gaccagatgg ctgttcacgt
     9841 accgctgacg ctggaagccc agctggaagc gcgtgcgctg atgatgtcta ccaacaacat
     9901 cctgtccccg gcgaacggcg aaccaatcat cgttccgtct caggacgttg tactgggtct
     9961 gtactacatg acccgtgact gtgttaacgc caaaggcgaa ggcatggtgc tgactggccc
    10021 gaaagaagca gaacgtctgt atcgctctgg tctggcttct ctgcatgcgc gcgttaaagt
    10081 gcgtatcacc gagtatgaaa aagatgctaa cggtgaatta gtagcgaaaa ccagcctgaa
    10141 agacacgact gttggccgtg ccattctgtg gatgattgta ccgaaaggtc tgccttactc
    10201 catcgtcaac caggcgctgg gtaaaaaagc aatctccaaa atgctgaaca cctgctaccg
    10261 cattctcggt ctgaaaccga ccgttatttt tgcggaccag atcatgtaca ccggcttcgc
    10321 ctatgcagcg cgttctggtg catctgttgg catcgatgac atggtcatcc cggagaagaa
    10381 acacgaaatc atctccgagg cagaagcaga agttgctgaa attcaggagc agttccagtc
    10441 tggtctggta actgcgggcg aacgctacaa caaagttatc gatatctggg ctgcggcgaa
    10501 cgatcgtgta tccaaagcga tgatggataa cctgcaaact gaaaccgtta ttaaccgtga
    10561 cggtcaggaa gagaagcagg tttccttcaa cagcatctac atgatggccg actccggtgc
    10621 gcgtggttct gcggcacaga ttcgtcagct tgctggtatg cgtggtctga tggcaaagcc
    10681 ggatggctcc atcatcgaaa cgccaatcac cgcgaacttc cgtgaaggtc tgaacgtact
    10741 ccagtacttc atctccaccc acggtgctcg taaaggtctg gcggataccg cactgaaaac
    10801 tgcgaactcc ggttacctga ctcgtcgtct ggttgacgtg gcgcaggacc tggtggttac
    10861 cgaagacgat tgtggtaccc atgaaggtat catgatgact ccggttatcg aggggggtga
    10921 cgttaaagag ccgctgcgcg atcgcgtact gggtcgtgta actgctgaag acgttctgaa
    10981 gccgggtact gctgatattc tcgttccgcg caacacgctg ctgcacgaac agtggtgtga
    11041 cctgctggaa gagaactctg tcgacgcggt aaaagtacgt tctgttgtat cttgtgacac
    11101 cgactttggt gtatgtgcgc actgctacgg tcgtgacctg gcgcgtggcc acatcatcaa
    11161 caaaggtgaa gcaatcggtg ttatcgcggc acagtccatc ggtgaaccgg gtacacagct
    11221 gaccatgcgt acgttccaca tcggtggtgc ggcatctcgt gcggctgctg aatccagcat
    11281 ccaggtgaaa aacaaaggta gcatcaagct cagcaacgtg aagtcggttg tgaactccag
    11341 cggtaaactg gttatcactt cccgtaacac cgaactgaaa ctgatcgacg aattcggtcg
    11401 taccaaagaa agctacaaag tgccttacgg tgcggtactg gcgaaaggcg atggcgaaca
    11461 ggttgctggc ggcgaaaccg ttgcaaactg ggacccgcac accatgccgg ttatcaccga
    11521 agtaagcggt tttgtacgct ttactgacat gatcgacggc cagaccatta ctcgtcagac
    11581 cgacgaactg accggtctgt cttcgctggt ggttctggat tccgcagaac gtaccgcagg
    11641 tggtaaagat ctgcgtccgg cactgaaaat cgttgatgct cagggtaacg acgttctgat
    11701 cccaggtacc gatatgcctg cgcagtactt cctgccgggt aaagcgattg ttcagctgga
    11761 agatggcgta cagatcagct ctggtgacac cttggcgcgt attccgcagg aatccggcgg
    11821 taccaaggac atcaccggtg gtctgccgcg cgttgcggac ctgttcgaag ctcgtcgtcc
    11881 gaaagagccg gcaatcctgg ctgaaatcag cggtatcgtt tccttcggta aagaaaccaa
    11941 aggtaaacgt cgtctggtta tcaccccggt agacggtagc gatccgtacg aagagatgat
    12001 tccgaaatgg cgtcagctca acgtgttcga aggtgaacgt gtagaacgtg gtgacgtaat
    12061 ttccgacggt ccggaagcgc cgcacgacat tctgcgtctg cgtggtgttc atgctgtgac
    12121 tcgttacatc gttaacgaag tacaggacgt ataccgtctg cagggcgtaa agattaacga
    12181 taaacacatc gaagttatcg ttcgtcagat gctgcgtaaa gctaccatcg ttaacgcggg
    12241 cagctccgac ttcctggaag gcgaacaggt tgaatactct cgcgtcaaga tcgcaaaccg
    12301 cgaactggaa gcgaacggca aagtgggtgc aacttactcc cgcgatctgc tgggtatcac
    12361 caaagcgtct ctggcaaccg agtccttcat ctccgcggca tcgttccagg agaccactcg
    12421 cgtgctgacc gaagcagccg ttgcgggcaa acgcgacgaa ctgcgcggcc tgaaagagaa
    12481 cgttatcgtg ggtcgtctga tcccggcagg taccggttac gcgtaccacc aggatcgtat
    12541 gcgtcgccgt gctgcgggtg aagctccggc tgcaccgcag gtgactgcag aagacgcatc
    12601 tgccagcctg gcagaactgc tgaacgcagg tctgggcggt tctgataacg agtaatcgtt
    12661 aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt ttatgggtgt tattcagagg
    12721 gcaaattctt cacggaaaat attaagaaag gcagggcgaa acgaggaaga agcttttcgc
    12781 cctggacaac attcctgctg acggcactac cataaaaaaa cacaacgctt tcaacaacct
    12841 tgcgtttttc atcttccgta gcggctcgga ataaatttac gttcttacat tccgtctcat
    12901 agtctttgcg aggcgcgtcc cagatagctg tcccagtctt tccataccgg ctgcaaaccc
    12961 tgagcggtta acacggcagc aaccgcttcc ggtctgcgat cgtcgtgcgg tgagaactgt
    13021 tccaactcgg ggtgattatc ggcatagcca cctggctgcg ttttcgagaa ggcgctgacg
    13081 ttattgatcg ccagcggaat aacgcgatcg cgaaaccacg gtgattcccg cgtggagagt
    13141 gacagttcaa tctccggtgc aagcagtcgg aaggcgcaga tggtttgcac taactggcgt
    13201 tcatccataa tcgacgcagg ctcaatgccg ccagtacacg ggcgcaggcg cggaaaggag
    13261 acagagtaac ggctttgcca gtaatgctgt tgcagccata gcaaatgttc ggcaaccata
    13321 tagcagtcaa cgcgccagtt gtcggaaagg ccaattagcg cgccgaggcc tatcttatca
    13381 atccccgcac gccccagccg atccggcgtt tccagccgcc agaagaagtc ctgttttttg
    13441 cctttcaaat gatggcgggt ataagtcgcc tcgtgatatg tctcctgata aaccatcacc
    13501 ccatccagac caagttgctt taactcggcg tattccgtct ccgccagcgg ttgcacttcc
    13561 atttgtagtg aagagaactg ttcacgcagg gcggggagat gacgacgaaa gtaatccatc
    13621 cccactttcg cctgatgttc accagtgact aatagcagat gttcaaagcc catctcccgt
    13681 atagcggcgc tttccctggc aatatccgct tcatccagcg ttttgcgctt gatgcgatta
    13741 ctcatggaaa atccgcagta cgtgcagtcg ttagcgcaaa gattggaaag ataaagcggg
    13801 acgtagaaac taactgtgtt gccaaatcgc tgacgggtca gacgctgcgc ctgttgggcc
    13861 agttgttcca gatagccact ggcggcaggc gataacagcg ccatcatgtc gtcgcgggtg
    13921 agttgcgagg catttagcgc ccgctctacg tcagcagccg ttttgccgtt gatacgcagg
    13981 cggatgtcgt cccagtccag ttgtcgccag cgattgctga aggttttcat gccgatgcct
    14041 ccagaaatcc ggtcagcggg ctggtggcat gagcaaaata actgcggctg cccggtccgg
    14101 actgacgtgc cagcaggcct acttctaccg ccagacgaaa tgccttcgcc atgttgacgg
    14161 gatcgtccgc gacggcaatc gccgtattca ctaacaccgc gtcggccccc atttccagcg
    14221 cctgcgtggc atggctggga acgccgatgc cagcatcgac aaccaccgga actgtggcct
    14281 gctggataat aatctccagc atggcacggg tttccagtcc ctgattcgag ccaatcggcg
    14341 cgccgagcgg catcaccgct gcacagccga cttcttccag acgtttacac aataccggat
    14401 cggccccgca gtaaggcagc acgacaaatc cctgttgtac cagcgtttcg gcggctttca
    14461 gggtttcgat gggatcgggc aacagccagc gggcgtcagg gtgaatctcc aattttagcc
    14521 agtttgtgcc taacgcttca cgtgccagat gggcggcgaa aatggcttct tccgctgttt
    14581 tcgccccgga agtattcggc agcagggcca cacccgccgc gataagcggt tcgaggatag
    14641 cgtcgttgtg ctggcgcaag tcgacacgtt tcatcgccag tgtcaccagc tggctgccgg
    14701 aagcgcggat cgcctccacc atcagttgtg aagaagcgaa tttccctgtg ccggtaaaca
    14761 gatgtgaatc aaacgttttg tccgcaatac gtaacatctc aaccccctgc aataacctga
    14821 aaaagcagga tctggtcgcc atcctgcacg atatgttgcg tccactgctc acgcgggacg
    14881 atttgctgat taatcgccag agccgcgccc gcttgtcgtt ggtcgagttg ctccagtagt
    14941 tcgtgaacag tttgcccggc ggcgcactgc atcggttgat cgttaaacag gatctgcatt
    15001 gcttcctccg cataccgggc aaccactggc gcggcgcaac gccaggctgc gccactggct
    15061 cgacttaccg tcgaacagtc ggagttctcc cgcaggcgtc tctataccgc ttaataactt
    15121 aatggcttcc agtgcctgca aagtgcccat caccccgacc accgggccaa ccacgcccgc
    15181 cgtgcggcag ttgcgttctg gctgctggtt atctggccac aggcagcggt aacacccctg
    15241 ctcccagggc ggtgtcagta ccatcaactg accgccaaat ccgaccgcgc tggcggtgat
    15301 aagcggcgtg ttgagtgcca cgcaggcggc attaatctcc tggcgagtcg ccatattgtc
    15361 ggtacagtcg agcaccacat cggcttgtgc aaccgcatct ttcaacgcct cacccgttaa
    15421 ccgttgttgt aatgctgtca gttgaatgtc gggattcaac tgtgtcagtc gctgttggct
    15481 gacctgcgat ttcgggctgt tgatatcttc agtggtaaag aggatttgtc gttgcagatt
    15541 gcttaaatgc acatcgtcgt catctgccag taccagcgtc ccgacgccag cccctgccag
    15601 gtatagcgca gcaggtgtac ccagcccgcc aagaccaata atcagcacct ggctgtcgag
    15661 cagtttttgc tgcccgtcaa gagcgatatc gtcgagcaga atttggcggc tataacgcat
    15721 aaagtcacgg tcattcatcg ccaactcctg caatttccag caactgtgcc gttgccaaac
    15781 gccagtctgc ggcttgagta atggcgctga cgacggcgat actgccgaca cccgttgcta
    15841 tcaccgcagg cgcgcgtgcc agactgatac cgccaatcgc cacggtggga taatccgcca
    15901 ggcgctcaac atgccgtgcc aactgttcca gcccctgcgg tgcagaaggc atctgtttgg
    15961 tttgcgtcgg gaacacatgt cccagcgcga tataagaggg gcgtgctgcc agcgcgacgt
    16021 cgatttccat atcgtcatgt gtcgaaacgc ccagccgcag gcctgccgcg cggatggcat
    16081 tgagatcggt ggcttgcaaa tcttcctgcc ccaaatggac gccatacgcc tgatgcttga
    16141 tcgcgagccg ccagtaatcg ttgataaaca atcgcgcgtt atagcggcgg cccagcgcaa
    16201 ttgccgccac gacatcggct tccacctctt catcgcgccg atctttgatg cgtagctgga
    16261 gagtacgtac gcctgcatcc aacagacgtt cgatccactg tacgctgtcc accaccgggt
    16321 acagtcctaa acgaaaaggt acaggaggaa aatcaggctg atacatcacg cttcctcctt
    16381 atgcaggtag atttctccgc ctctggcacg gaagttctcc gacatatccg ccattcccac
    16441 ttcaatggtt tgcgcggcgg cgtaatcgcg tacttcctgg ctgattttca tcgagcagaa
    16501 ttttggccca cacatggagc aaaaatgggc gactttgccg gactcttggg gcagggtttc
    16561 atcgtgataa gcgcgggcgg taaacgggtc gagggccaga ttaaactggt cttcccagcg
    16621 aaattcgaag cgggctttcg acatggcgtt atcgcgaatt tgcgcgcccg gatgcccttt
    16681 tgccaggtca gcagcgtggg cggcaatctt ataagtgata agcccctgct taacatcttc
    16741 tttattgggc agacccagat gctcttttgg cgttacgtaa cagagcatcg cgcagccaaa
    16801 ccagccaatc atcgccgcac caatccccga cgtgaagtgg tcatagcccg gtgcaatatc
    16861 ggtagttagc ggccccagag tgtaaaacgg cgcttcgtgg cagtgctcta actcctcggt
    16921 catattgcgg cggatcatct gcatcggcac atggcctggg ccttcaatca tcacctgtac
    16981 gtcatattcc caggcgattt tggtcagttc gcccagcgta tgcaattcgg caaactgcgc
    17041 ttcatcgttg gcgtcctgaa tagaaccggg gcgcagaccg tcgcccagcg acagcgaaac
    17101 gtcataagcg gcacagattt cacaaatttc gcggaagtgt tgatagagga aattttcctg
    17161 atgatgggag aggcaccatt tcgccataat cgaaccgccg cgagagacga taccggtcag
    17221 gcgtttcgcg gtcatcggca catagcgcag cagtacgccc gcatggatag tgaagtaatc
    17281 cacaccttgc tcggcctgtt ccagcagcgt gtcgcggaac gcttcccagg taagatcttc
    17341 ggcgatcccg ttaaccttct ccagcgcctg gtagatcggc actgtaccga tcggcaccgg
    17401 actgttacgc aaaatccact cgcgggtttc gtgaatatag cgaccggtgg agagatccat
    17461 caccgtatcc gctccccagc gcgtggacca taccagcttt tccacttctt cttcgatgga
    17521 agaggtgacc gccgagttgc cgatattggc gttaactttt accaggaagt tacggccaat
    17581 aatcatcggc tccgattccg gatgattaat gttggccggg ataatcgcac gtccggcagc
    17641 aacttcatca cggacaaatt ccgcagtgat attttccggc agatgtgcgc caaagctcat
    17701 tcccggatgc tggtggcgta aaacctcgct acggatgcgc tcgcggccca tattctcgcg
    17761 gatggcgatg aattccattt ccggcgtgat gatgccctgg cgggcgtagt gcagttgggt
    17821 gacacggcgt cctgttttgg cgcgttttgg cgttaataag ccgctaaaac gtagctcgtc
    17881 gagaccatca tctgccagcc gcgttctggt gtaatcggaa ctgcgcacgg taagttcttc
    17941 ggtatcggcg cgcgcatcga tccacggctg gcgtagtttt gccagccctt gctgcacgtt
    18001 aatggcgatc tgcggatcac catacgggcc ggaggtgtcg tagaccggaa tcgcttcgtt
    18061 ttcttcgtac tgcggctgtt ctttgctacc gccaattagc gtcgggctaa gctggatctc
    18121 acgcatcggc acgcgcacgc cggggtgtgt gccagtgata taaatgcgtt ttgagttggg
    18181 aaaggcggtg ccttccaggg tgtcgataaa atgttgggcc cgggcgcgtt gttcgcggcg
    18241 ggtcagtttt gttgcagaca tagctcattc caaaaagtta aggacgtggc ttgtcagacg
    18301 acggatgaag caagagacga tcgccgcaaa ggcgatgcga tagcagatta actcttgttc
    18361 ccttcgcagg tattagcctg atcaggttcc gcggatcccg aataaacggt ctcagccagt
    18421 taaggcactc cgacaagaaa ttagccccca caaacggggc gttgaatgta aattacgcgt
    18481 taacagcgca gaactcaagc aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc
    18541 agcttatctt ccagcacaaa gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt
    18601 tgaaactcaa ggtaattatc atgatcgatg gcggtttcca ggctggaatc gtagtaatcc
    18661 ataatctgtt gggtattggc ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc
    18721 ccgttgcctt ccagcttatg aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag
    18781 taatcgacca ggctctgaca aaaatcatca agggcttttt cgtttagcct catgtacgat
    18841 tctttgccag gcttaatgcc aaccagattg tagtaagcca cgagcagatg cttacgtaca
    18901 tgtagccagc gatcaaccag tttgttactt cctctgacgc gctccgtcag gttatcgagc
    18961 tggttaagca tgattgactc cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa
    19021 aaaccatgtt aaacatgcca gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt
    19081 gaaaaattag atcacggctg gtgggtcgtc agccatgaac aaaaattatg gttgccgaag
    19141 ggagaattgc catatggcga agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa
    19201 atcggcgaat ggcaggggga acctgtttgg ttaatacaac agcagcggcg tcacgatatg
    19261 gggtcggtac gtcaggtcat tgatctcgat gttgggctgt ttcaactggc cggacgtggc
    19321 gtgcaactgg cggagtttta ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg
    19381 tatccgagca aaaccgaatg ggcgatgctg tgtagccatt gccgtgagcg ttactacccg
    19441 caaatcgccc cctgcattat tgttgccatc cgtcgcgatg attcgctcct cctcgcccag
    19501 catacccgcc atcgtaacgg tgtccataca gtacttgccg gattcgtcga agtgggcgaa
    19561 accctcgagc aggcagtcgc gcgggaagtg atggaagaga gcggaattaa agttaaaaac
    19621 ttgcgttacg tgacttctca gccgtggccg tttcctcagt ctttaatgac cgcgtttatg
    19681 gcggaatatg acagcggcga catcgtgatc gacccgaaag aattgctcga ggcgaactgg
    19741 tatcgctatg acgatttgcc gttactcccg ccgcccggca ccgtagcgcg ccgtctgata
    19801 gaagatactg tggcgatgtg tcgggcagag tatgagtgat gatacactga ccgcctgacg
    19861 cactaaggaa cagccaaaat gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg
    19921 cgccagcccg ttgatgtcac tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg
    19981 gaatataaag ccacgcgcgc ccaggcgggc gattttatgt cgctgtgcaa aaacgccgag
    20041 ctggcgtgcg aagtgacttt gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc
    20101 ttttccgata tcctcaccgt gccggacgcg atggggttag ggctctattt tgaagccgga
    20161 gaaggtccgc gttttacctc gccagtcacc tgcaaagccg acgtcgataa actgccaatt
    20221 ccggacccgg aagatgagct gggttacgtg atgaacgcgg tgcgtaccat tcgtcacgaa
    20281 ctgaaaggcg aagtgccgct gattggtttt tccggcagcc cgtggacgct ggcaacctac
    20341 atggtggaag gcggcagcag caaagccttc accgtgatca aaaaaatgat gtatgccgat
    20401 ccgcaggcgc tgcacgctct gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat
    20461 gcgcagatta aagccggtgc tcaggcagtg atgattttcg acacctgggg cggcgtgctt
    20521 accgggcgcg attatcaaca gttctcgctc tattacatgc ataaaattgt tgatggttta
    20581 ctgcgtgaaa acgacggtcg ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag
    20641 tggctggaag ccatggctga aaccggttgc gatgcgctgg gcctcgactg gacaacggac
    20701 atcgccgatg cgcgccgccg tgtgggcaat aaagtcgcgt tgcagggtaa tatggatccg
    20761 tcgatgctgt acgcgccgcc tgcccgcatt gaagaagaag tagcgactat acttgcaggt
    20821 ttcggtcacg gcgaaggtca tgtctttaac cttggtcacg gcattcatca ggatgtgccg
    20881 ccagaacatg ctggcgtgtt cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc
    20941 taagagcctg atccatcagg ctattttatt tgccattttg aggccgggca gtgctcaaaa
    21001 tcctcacgta ctacgtgtac gctccggttt ttccgcgctg tccggcctca aactgccttc
    21061 aacaattacg cctgttgaac caggctctaa ggagcgatta tggatctcgc gtcattacgc
    21121 gctcaacaaa tcgaactggc ttcttctgtg atctgcgagg atcgactcga taaagatcca
    21181 ccggatctga tcgccggagc cgatgtcggg tttgagcagg gcggagaagt gacgcgagcg
    21241 gcgatggtgc tgctgaaata tccctcgctt gagctggtgg agtataaagt tgcccgcatc
    21301 gccaccacca tgccttacat tccaggtttt ctttccttcc gcgaatatcc tgcgctgctg
    21361 gcagcgtggg agatgctgtc gcaaaagccg gatttagtgt ttgtcgatgg tcatgggatc
    21421 tcgcatcctc gccgtcttgg cgtcgccagc cattttggct tattggtgga tgtgccgacc
    21481 attggcgtgg cgaaaaaacg gctctgcggt aaattcgaac cgctctccag caaaccgggc
    21541 gcgctggccc cactgatgga taaaggcgag cagctggcct gggtctggcg cagcaaagcg
    21601 cgctgtaacc cgttgtttat cgctaccggc catcgggtca gcgtggacag cgcgctggcg
    21661 tgggtacaac gctgcatgaa aggctatcgt ctgccggagc caacgcgctg ggcggacgcg
    21721 gtggcctcgg aacgtccggc gttcgtgcgc tatacagcaa atcagcccta attcaggtaa
    21781 actgcggata atttccgtat ttgagaactc aacatgttac aaaacccaat tcatctgcgt
    21841 ctggagcgcc tggaaagctg gcagcatgtc actttcatgg cttgcttatg cgaacgcatg
    21901 taccccaatt acgccatgtt ctgccagcaa accggttttg gtgatgggca aatttaccgt
    21961 cgtattctcg atctcatctg ggaaacgctg accgttaaag acgcaaaagt aaatttcgac
    22021 agccaactgg agaaatttga agaagcgatt ccttcagccg acgatttcga tctgtacggc
    22081 gtttatccgg caatcgatgc ctgcgtggcg ttaagtgaac tggtccattc gcgtttgagt
    22141 ggtgaaacgc tcgaacacgc ggtggaagtg agtaagacct ccatcacgac cgttgcgatg
    22201 ctggaaatga ctcaggctgg tcgcgaaatg ggcgatgaag agcttaaaga aaacccagct
    22261 gtagagcaag aatgggacat tcagtgggaa atattccgac ttttagctga gtgcgaagaa
    22321 cgcgatatcg agctgataaa aggccttagg gcagacctgc gtgagtcggg tgagagcaat
    22381 attggtataa tttttcagca ataagaccag aaaacgtgat ttaacgcctg atttgtcgta
    22441 cctggagtct tccctttcgc cccccgtctg gtctacattt ggggggcgaa aaaaagtggc
    22501 tatcggtgcg tgtatgcagg agagtgctat tctggcattt ccgtcgcact cgatgcttag
    22561 caagcgataa acacattgta aggataactt atgaacaaga ctcaactgat tgatgtaatt
    22621 gcagagaaag cagaactgtc caaaacccag gctaaagctg ctctggagtc cactctggct
    22681 gcaattactg agtctctgaa agaaggcgat gctgtacaac tggttggttt cggtaccttc
    22741 aaagtgaacc atcgcgctga gcgtactggc cgcaacccgc agaccggtaa agaaatcaaa
    22801 attgccgcag ctaacgtacc ggcatttgtt tctggcaagg cactgaaaga cgcagttaag
    22861 taagattgcg tggcagtgaa cagttttaac gaaggggtgg tttcacccct tttgtctttc
    22921 tggcgtcgat cattgatgct ggcaggtgcg ctgtttctca ctgcctgtag ccacaactct
    22981 tcacttcctc cctttaccgc cagtggattt gctgaagacc agggcgcggt acgcatctgg
    23041 cgaaaagaca gcggcgataa tgtgcatctg cttgccgtgt ttagcccgtg gcgcagtggc
    23101 gataccacga cgcgagagta tcgctggcag ggcgataacc tcacgctcat caatatcaat
    23161 gtttacagca aaccgccggt gaacattcgc gcgcgttttg acgatcgcgg cgatctgagc
    23221 tttatgcaac gtgaatccga tggggaaaag cagcagcttt ctaacgacca aatcgattta
    23281 taccgttatc gtgctgatca gatccgccag attagcgatg ccttacgtca ggggagagtc
    23341 gtgctgcgcc aggggcgctg gcatgcgatg gaacagaccg tgaccacctg cgaagggcaa
    23401 accattaaac ctgatttaga ttcgcaggcg atagcgcata tcgagcgccg ccagagccgc
    23461 tcttctgttg atgtcagcgt ggcatggctg gaagcgcccg aaggttcgca attactgtta
    23521 gtggcaaact ctgatttctg tcgctggcaa cccaacgaga aaacgttctg atttaccagt
    23581 ggcccatacc catatgaccg ccaccaccgc agccgccgta gcccatgccc attccggctc
    23641 cgcgcggaat acccgcttca gccatcgcga tatctcgttt cacccgtaac tcatctaacg
    23701 actgacgcaa attctccatc tctttggcga ccgcgttaat tttgctgcta tccggtgggt
    23761 tcgcggctaa cagggcattg tattcataac gcttcgtcac cagttgctgt tgcagtgcgc
    23821 tgctttgagc gtaaaagtca ttatggattt tctgccacgc tgtctgttgt tcgctggtca
    23881 aaggcgcggc attttgctgc cacataccgt gtccgccgtg agcaaatgca gatgtcgatc
    23941 ccatcgccat tgctgaaagc gccatcatta ccagggcaat tttcgtgttc cgtttcatgg
    24001 ttaatcctcc agtggttgtc ttacttcggg tattgcatct tccgtgccaa cgatgaaacg
    24061 ctgatatgac gggtaatctg gcatgataaa cgagtaaaaa tgactcgcct gctgcgggta
    24121 gcgagtcatt tttactcatt gaaacttaag cctttgtgtt acagcgcagg gtaagcgctg
    24181 ataaaagatg gcatgatttc tgctgtcaga aagggatgag caggcaaaga agaagatgcg
    24241 ttttatgcaa cgttctaaag actccttagc taaatggtta agcgcgatcc tccccgtggt
    24301 cattgttggg ctggtagggt tgtttgcggt gacggtgatt cgcgattatg ggcgcgagac
    24361 tgccgccgcc agacaaacgc tgctggaaaa aggcagtgta cttattcgcg ctcttgaatc
    24421 cggctcgcgc gtcggcatgg ggatgcgcat gcatcatgcg cagcagcagg ccttactgga
    24481 agaaatggcc gggcagcctg gagtacgttg gtttgcggtc acggatgaac aaggaacaat
    24541 cgtgatgcat agcaactccg gcatggtggg aaaacagctt tattcccctc aggaaatgca
    24601 gcagttacat ccgggagatg aagaagcgtg gcggcggatc gatagcgcag acggcgagcc
    24661 tgttctggaa atttatcgcc agtttcaacc gatgtttgct gctggaatgt accggatgcg
    24721 ccatatgcag cagtatgccg cgacaccaca agcaattttc atcgctttcg acgccagtaa
    24781 tattgtgagt gccgaagatc gtgagcagag aaacaccctg attattctct tcgccctggc
    24841 gacggtcttg ctggcaagcg tattgtcatt cttctggtat cgccgctatc tgcgctcgcg
    24901 ccagcttcta caagatgaaa tgaagcgcaa agagaagctg gtggcgctgg ggcatcttgc
    24961 ggcaggcgtt gcccacgaaa tccgtaaccc actttcctcg attaaaggac tggcgaaata
    25021 ctttgccgag cgcgcgcctg cagggggaga agcgcatcaa ctggcgcagg tgatggcgaa
    25081 agaggccgac cgtttaaacc gcgtggtaag cgagttgctg gaactggtta agccaacgca
    25141 tctggctttg caggcggtgg atctcaacac gctgattaac cactcattac agctggtaag
    25201 ccaggatgca aacagccggg agatccagtt acgctttacc gccaacgaca cattaccgga
    25261 aattcaggcc gatccggaca ggctgactca ggtcctgttg aatctctatc tcaatgctat
    25321 tcaggcgatt ggtcagcatg gcgtgattag cgtgacggcc agcgaaagcg gcgcgggcgt
    25381 gaaaatcagc gttaccgaca gcggtaaggg aattgcggca gatcagcttg aagccatctt
    25441 cactccgtac ttcaccacca aagccgaagg caccggattg gggctggcgg tcgtgcataa
    25501 tattgttgaa caacacggtg gtacaattca ggtcgcaagc caggagggaa aaggctcaac
    25561 gttcaccctc tggcttccgg tcaatattac gcgtaaggac ccacaaggat gacgcacgat
    25621 aatatcgata ttctggtggt ggatgatgac attagccact gcactatttt gcaggcttta
    25681 ctgcgcggct ggggctataa cgtcgcgctg gcgaacagcg ggcgacaggc gctggagcag
    25741 gtgcgggaac aggtttttga tcttgtgctt tgcgatgtgc gaatggcgga gatggacggc
    25801 atcgccacgc tgaaagagat caaagcgtta aacccggcaa ttccggtgct gattatgact
    25861 gcgtactcca gcgtcgagac ggcggtagag gcactgaaaa ctggggcgca ggattatctc
    25921 atcaagccgc tggatttcga taacctacag gcgacgctgg aaaaggcgct cgcgcatacg
    25981 cacagtattg atgctgaaac acctgcggtg actgccagcc agttcggtat ggtcggtaaa
    26041 agcccggcga tgcaacacct gctcagtgaa atcgccctcg tcgcgccatc ggaagccacg
    26101 gtgctgatcc acggcgattc cggcaccggt aaagagctgg tcgccagggc gattcacgcc
    26161 agtagcgcac gtagcgaaaa accgctggta acgctcaact gtgcggcact caacgaatcc
    26221 ttgctggaat ctgaattgtt cggtcacgaa aaaggggcgt ttactggagc cgataaacgc
    26281 cgggaagggc gctttgttga ggcggacggc ggcacgctgt ttctcgatga aattggcgat
    26341 atctcgccga tgatgcaggt gcgtctgctg cgtgcgattc aggagcgcga agttcagcgt
    26401 gtcggtagca accagactat ctcggttgat gtccggctga ttgcggcgac ccatcgcgat
    26461 cttgccgcag aggtgaatgc cgggcgtttt cgccaggatc tctactatcg cctgaatgtg
    26521 gtggcgattg aagtaccatc gctgcggcaa cggcgggaag atatccctct gctggctggc
    26581 cattttcttc agcgctttgc cgagcgtaat cgcaaggcgg taaaaggctt tacgccacag
    26641 gcgatggatt tgctgatcca ctacgactgg ccgggaaata ttcgtgaact ggaaaacgcg
    26701 gtggaacggg cggtggtgct gctgaccggg gaatatattt ccgaacgcga gctgccgctg
    26761 gcgattgcca gtacgccgat cccgctgggg caaagtcagg atattcagcc gctggtggaa
    26821 gtggaaaaag aggtgattct ggcggcgctg gagaaaacgg gcggcaacaa aaccgaagcc
    26881 gcccgtcagt tagggatcac gcgcaaaacg ctattggcaa aactgtcgcg ttagttctgc
    26941 tcgcgttcga tggcgcgcca accgatatct ttccggcaga agcaatcatc ccagtggata
    27001 tcggtcatta aggcgtaggc gcgtttctgc gcttctgcca cggtatgacc cagcgcggtg
    27061 acgcacagta cgcgcccgcc gctggttacc acctgctcgt catccgccag ttttgtgccc
    27121 gcgtggaaca ctttgccgtc agccacttct tccagcggca ggccgtggat cacatcgccg
    27181 gtgcggtaat cgcccggata tccacccgca gccatcacca cgctgagaga agcacgttca
    27241 tcccactctg acgttttctc gtccagtttg ccttcacagg ccgccaggca gagctcaacc
    27301 agatcggact tcatgcgcag cataatcggc tgggtttccg gatcgccaaa gcggcagtta
    27361 aattcaataa ccttcggatt gccctgtttg tcgatcatca ggcccgcata gagaaaaccg
    27421 gtgtaggtgt tgccttccgc cgccatgcct ttcacggttg gccagatgat acgttccatg
    27481 gtgcgctgat gaacttcatc ggttactacc ggcgcggggg agtaagcgcc catcccgccg
    27541 gtgtttggtc cgttatcttt atcgcctacg cgtttgtgat cctggctggt agccatcggc
    27601 agcacatgct cgccgtccac catcacgata aagctcgctt cttcgccatc gaggaactct
    27661 tcgataacga tgcgatgacc cgcgtcgcca aaagcgttgc ccgccagcat atcgtgaaca
    27721 gccgcttccg cttcttccag cgtcatcgcc acgataacgc ctttcccggc agccagaccg
    27781 tccgctttaa tgacgattgg cgcgcctttc tcacgcagat acaccagcgc aggttctacc
    27841 tcggtgaagt tctggtattc cgccgtaggg atcttatggc gggccaggaa atctttggta
    27901 aacgcttttg agccttccag ttgggccgca cctgcggttg ggccgaagat tttcagcccg
    27961 gcggcgcgga aggtatcgac cacgcctttc accagcggcg cttccgggcc gacgatggtc
    28021 agatcaatct tttcgttttg tgcgaaatcc aacagcgccg ggatatcggt cacgccgata
    28081 gcaacgtttt gcagcgcggg ttccagcgca gtgcctgcat tacccggagc agcaaaaaca
    28141 gtctcaacca gcggcgactg ggccgctttc caggccagcg cgtgctcgcg cccgccgtta
    28201 ccaatcacta atactttcat ctattgctcc attaatggcg gaagtggcgc atgtcggtga
    28261 agagcatcgc aataccgtgc tcgtcggcgg cggcaatcac ttcgtcatca cggatggaac
    28321 cgccaggctg gattacgcag gtcacgcccg cagcggcggc ggcatcaata ccgtcgcgga
    28381 acgggaagaa cgcgtcagaa gccatcgagg aacctttcac ttccaggcct tcatcggcag
    28441 ctttaatacc ggcgattttc gcggagtaca cgcggctcat ctggcccgcg ccaataccga
    28501 tggtcatatt gtttttggca tagacgatag cattggattt cacgaatttc gccactttcc
    28561 agcagaacag cgcatcacgc agttcctgtt cggtcggctg acgttgggtg acgacgcgca
    28621 gttcttccgc accgaccatc cccaggtcac gatcctgaac cagcagaccg ccgttcacgc
    28681 gtttgaaatc aagacccgga acacgctcgc cccactgacc gcaggtcaga acgcgtacgt
    28741 tctgcttggc ggcggtgatt ttcagggctt cttcgctggc ggaaggcgca ataatcactt
    28801 cgacaaactg acgagaaatg atggcctgtg cggtttccgc atccagctcg cggttaaagg
    28861 caatgatgcc gccgaatgcg gaggttgggt cggttttgta cgcgcgatcg taagcatcaa
    28921 gaatggaatt gccgatagct acaccgcaag ggttggcgtg cttcacaatc acacatgccg
    28981 gctcggcgaa ctctttcacg cactccagcg ccgcatcggt atcggcgatg ttgttataag
    29041 agagggcttt accctgaacc tgggttgcgg tagcaacgga ggcttctttc acattctctt
    29101 ctatatagaa ggcagcctgc tggtggctgt tctcgccgta acgcatatcc tgcttcttaa
    29161 tgaagttcag gttcagcgtg cgtgggaagc gaccggcggc ttctttgctt tcaccgtggt
    29221 acgccggaac catgctgccg aagtagttgg caatcatgct gtcgtaggcg gcagtgtgtt
    29281 cgaaggcttt gatggcgagg tcgaaacggg ttgcaagcgt cagcgatcct tcgttgtcat
    29341 ccatctcttt aataatggcg tcgtagtcgc tgctcttcac cacgattgcg acatctttat
    29401 ggttcttagc ggcggagcgc accatggtcg ggccgccgat atcgatgttc tcaaccgcat
    29461 cttccagcga gcagccttca cgagcaacag tctgggcgaa cggatacagg ttaacaacca
    29521 ccatatcgat aggctggatc tgatgttctt ccataatggc atcgtcctgg ccgcgacggc
    29581 ccagaatgcc accatgtact ttcggatgca gggtcttcac gcgtccatcc atcatctccg
    29641 ggaaaccggt gtaatcggaa acttcggtta ccggcagacc tttttctgct aacagacggg
    29701 cagtgccccc tgtagacagc agctccacac cgcgtgcgga aagtgcctgg gcgaattcga
    29761 cgataccggc tttgtcagaa acactgagca gagcgcggcg gactggacga cgttgttgca
    29821 tggtaaatcc cctgggtttg actattacag agagcgttag ctgaattttt cgcgaaaaac
    29881 tcagctaacg cccctaacgg ggcatcctta tttttcgccc gcattgtaac gaaaacgttt
    29941 gcgcaacgct cgcgaatttt tctctttcaa tggtgatcac aattttgaca gtggttaccg
    30001 tgggaaaaat acagaaatta cattgatgat tgtggataac tctgtgcgta aaaaggtata
    30061 aagcggggtt ttgctgggga atgcagcagt cagtcatttt tctgcaattt ttctattgcg
    30121 gcctgcggag aactccctat aatgcgcctt catcgacacg gcggatgtga atcacttcac
    30181 acaaacagcc ggttcggttg aagagaaaaa tcctgaaatt cagggttgac tctgaaagag
    30241 gaaagcgtaa tatacgccac ctcgcgacag tgcgctaaag cgcgtcgc
//
LOCUS       DAZOYF010000032        30224 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZOYF010000032 DAZOYF010000000
VERSION     DAZOYF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.032"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(33..2480)
                     /gene="glgP"
                     /locus_tag="ACHUOF_001381"
     CDS             complement(33..2480)
                     /gene="glgP"
                     /locus_tag="ACHUOF_001381"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR0610630.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="ACHUOF_001382"
     CDS             complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="ACHUOF_001382"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR0610631.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="ACHUOF_001383"
     CDS             complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="ACHUOF_001383"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR0610632.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="ACHUOF_001384"
     CDS             complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="ACHUOF_001384"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR0610633.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="ACHUOF_001385"
     CDS             complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="ACHUOF_001385"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR0610634.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9674..10777)
                     /gene="asd"
                     /locus_tag="ACHUOF_001386"
     CDS             complement(9674..10777)
                     /gene="asd"
                     /locus_tag="ACHUOF_001386"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0610635.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10969..11562
                     /gene="yhgN"
                     /locus_tag="ACHUOF_001387"
     CDS             10969..11562
                     /gene="yhgN"
                     /locus_tag="ACHUOF_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR0610636.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="ACHUOF_001388"
     CDS             complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="ACHUOF_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0610637.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="ACHUOF_001389"
     CDS             complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="ACHUOF_001389"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR0610638.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="ACHUOF_001390"
     CDS             complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="ACHUOF_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR0610639.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="ACHUOF_001391"
     CDS             complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="ACHUOF_001391"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR0610640.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15666..16703)
                     /locus_tag="ACHUOF_001392"
     CDS             complement(15666..16703)
                     /locus_tag="ACHUOF_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR0610641.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17036..17524
                     /gene="yhhY"
                     /locus_tag="ACHUOF_001393"
     CDS             17036..17524
                     /gene="yhhY"
                     /locus_tag="ACHUOF_001393"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0610642.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="ACHUOF_001394"
     CDS             complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="ACHUOF_001394"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR0610643.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19445..19885
                     /locus_tag="ACHUOF_001395"
     CDS             19445..19885
                     /locus_tag="ACHUOF_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR0610644.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="ACHUOF_001396"
     CDS             complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="ACHUOF_001396"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0610645.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20612..21545)
                     /locus_tag="ACHUOF_001397"
                     /pseudo
     CDS             complement(20612..21545)
                     /locus_tag="ACHUOF_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="ACHUOF_001398"
     CDS             complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="ACHUOF_001398"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR0610646.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="ACHUOF_001399"
     CDS             complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="ACHUOF_001399"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR0610647.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="ACHUOF_001400"
     CDS             complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="ACHUOF_001400"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFR0610648.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25144..25857)
                     /gene="livF"
                     /locus_tag="ACHUOF_001401"
     CDS             complement(25144..25857)
                     /gene="livF"
                     /locus_tag="ACHUOF_001401"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR0610649.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25859..26626)
                     /gene="livG"
                     /locus_tag="ACHUOF_001402"
     CDS             complement(25859..26626)
                     /gene="livG"
                     /locus_tag="ACHUOF_001402"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR0610650.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26623..27900)
                     /gene="livM"
                     /locus_tag="ACHUOF_001403"
     CDS             complement(26623..27900)
                     /gene="livM"
                     /locus_tag="ACHUOF_001403"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR0610651.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27897..28823)
                     /gene="livH"
                     /locus_tag="ACHUOF_001404"
     CDS             complement(27897..28823)
                     /gene="livH"
                     /locus_tag="ACHUOF_001404"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR0610652.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28871..29980)
                     /gene="livK"
                     /locus_tag="ACHUOF_001405"
     CDS             complement(28871..29980)
                     /gene="livK"
                     /locus_tag="ACHUOF_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR0610653.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT         7311 a         8424 c         7626 g         6863 t
ORIGIN      
        1 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc gatatgccag
       61 atatgatcag cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
      121 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcgtc gactttatcc
      181 tgacaatcga catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc
      241 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
      301 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
      361 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaaaataaa gatattgtca
      421 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
      481 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccctgc cagcgaaatc
      541 tgttcagaca gatctgccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
      601 atgaacacga ctttcagctt atcgccaatc tgcggatcgt tgttgatcac tttcgctacg
      661 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
      721 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
      781 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
      841 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
      901 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
      961 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
     1021 tcgtccagca cggctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca
     1081 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
     1141 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
     1201 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
     1261 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
     1321 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
     1381 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
     1441 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
     1501 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
     1561 gttttatgca actgataatg acggcttaaa atgtcctgaa tggtcgagga aaccaagaag
     1621 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
     1681 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
     1741 aatttaccga ggttaatttc gctactggct tgcgcactcc acaaacgcag cgtgttggtc
     1801 gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
     1861 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
     1921 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
     1981 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
     2041 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
     2101 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
     2161 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
     2221 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
     2281 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
     2341 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
     2401 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
     2461 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
     2521 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
     2581 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgccgaat agcccgtaac
     2641 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
     2701 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
     2761 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
     2821 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
     2881 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
     2941 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagccccgg taaggcttcc
     3001 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
     3061 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc tcttattttc cgctttatct
     3121 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
     3181 ttctcgtcca cgccgttcag tacgcctgaa agacgccctt cacggtgacg ctgttgcaac
     3241 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
     3301 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
     3361 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
     3421 gcataaaaca tgccttgata ggccaggttg tgcacagtaa acaccgactt cgcaggacgc
     3481 ccgcgcgccg ccagatacgc aggcgcaaga cctgcatgcc agtcgtgcac atgcaccaca
     3541 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
     3601 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
     3661 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
     3721 aacaacagcg tgatatgccc ggcgaaagta tcacgacggg atactacctg cgcatcggtc
     3781 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
     3841 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
     3901 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
     3961 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
     4021 cttcctctgc gttttcacca atcaccatgc cttccgggat aacacaagca cgatcgatga
     4081 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
     4141 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
     4201 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
     4261 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
     4321 tgcgatcgta catatccagt tccggcacca cagaggccag atcgagattc gctttccagt
     4381 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
     4441 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
     4501 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
     4561 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
     4621 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
     4681 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
     4741 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
     4801 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
     4861 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
     4921 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
     4981 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccagagt
     5041 tgatgcagtt agacagcgca aagtcaataa tgcggaactt accgccgaag tgtacggccg
     5101 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
     5161 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
     5221 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
     5281 ctgccagaca accgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
     5341 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
     5401 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
     5461 aggttgagca tatcgattta gccaacggac attgccgtca ccttcttccc accagcgatt
     5521 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
     5581 accactgctt gcctgcgacc agtccaacca gtttaattgg ttatcctgac agtaggcatt
     5641 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtgccctg
     5701 ggagagcaac aacgttgtta acagggcatg aatgctgtcg cgccgccgtt caaccaggtc
     5761 aagagtaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
     5821 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
     5881 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
     5941 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
     6001 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
     6061 cgggaaattt cccacctgat aaccactagg agcgatatcc cacggttcag caattaactt
     6121 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
     6181 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
     6241 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
     6301 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
     6361 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
     6421 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
     6481 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
     6541 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
     6601 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
     6661 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc
     6721 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
     6781 cgtgcgcggc ggggcgtcat cttcccagtc atagtgatca accaccacta cgcatttcgg
     6841 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
     6901 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
     6961 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
     7021 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgttccctg gcaagtcata
     7081 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
     7141 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
     7201 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
     7261 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
     7321 ccattgcctg cattactgcc gtgatagtgt atggaatcgg tattgaggat ttcacgccat
     7381 ttacctggct gattaatgcc gaagcgataa tcatgacgcg gcaccggcgt aaagttactg
     7441 gcaacgataa tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
     7501 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
     7561 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
     7621 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
     7681 tgggcaaatt cgttccccat gaacagcagt ttcttgcccg gaaatgccca catccagcca
     7741 tagtaagcgc gcaggttagc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
     7801 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
     7861 ttgtagagca tcccgaaggt cagtttatcg tgatgatact gacgataaat cgggtcgagc
     7921 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
     7981 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
     8041 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
     8101 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
     8161 tcacgataaa tcatcgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
     8221 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
     8281 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
     8341 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
     8401 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
     8461 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
     8521 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
     8581 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
     8641 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
     8701 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
     8761 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
     8821 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
     8881 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc
     8941 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
     9001 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
     9061 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
     9121 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
     9181 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
     9241 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
     9301 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
     9361 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
     9421 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
     9481 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
     9541 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
     9601 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
     9661 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
     9721 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
     9781 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
     9841 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
     9901 cttcgacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
     9961 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    10021 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
    10081 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    10141 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    10201 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
    10261 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
    10321 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
    10381 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    10441 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
    10501 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    10561 gacaggtcac aatgatatcg agggccttta gcgcctctag atcaaaggca tcctgaagtg
    10621 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
    10681 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    10741 ccataccgcg ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg
    10801 tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagctgcc
    10861 aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc
    10921 tttgttagca cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc
    10981 atttctgcag cagttttatt gatcctgatt atggatccgc tcggaaacct gcctattttc
    11041 atgtccgtac tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg
    11101 cttattgctc tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt
    11161 ctcagcctgc gagcagaaac cgtctccatt tctggcggca tcattctgtt tctaatcgcc
    11221 attaagatga ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag
    11281 ccatttatcg tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg
    11341 atgttgttgt ctcatcagta cccgaatcag atgggacatc tggtgattgc tctgctgctg
    11401 gcctggggcg gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc
    11461 gagaaagggg tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaact
    11521 cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt aacgaggaga gtttttcgaa
    11581 aagagcccgg tagcgtcaca actaccgggc gaacaaactt aactcaacag ctggaacgca
    11641 atcatcccaa cgatggcacc aacagtgccg aggatggttt ccatcatggt ccaggttttc
    11701 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc agcgtcgtta
    11761 acgtggctga caacaatcga accaccagcg atacaaatcg acagtgccgc catttgcgca
    11821 ccagagtagt tcagttgttc aatgactggc atcaccagtc ccaccgccgt taaacaggct
    11881 acggtggcag ccccctgaat gatgcgcact gcagcagcca gcacgaagca ggtgatagca
    11941 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc
    12001 agcacctgtt tgaacacgcc gcctgcacca atcaccagca gaatgatccc cgccggttgc
    12061 agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg
    12121 ccataaatcg ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg
    12181 aaccattcgt aagcggtaga gccttctggc acaaaacgcg cggcaatggt tttcagcccc
    12241 accagcacca gcggcaacag gatcagcgac aggctgaatc cgaaagatgg cattttacct
    12301 tcgccgagat gcggttcgct gatgtcgtca ggaatatgca gctcaacgta acggctgatg
    12361 aagttacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc
    12421 aggatcatcc agccaaagtc ggcattcatc tgcgatgcca gcagcattgg cgctggccca
    12481 ggcaccagga acgctgccgc ggccgccacg cctgcgaata atgggattac cagcttcacc
    12541 aggttcgtac cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc
    12601 acttcaaaga acagcggtag cgcacagacc agccccgcaa ggccgatggc ataatgcgcg
    12661 cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgactgc gccggtttca
    12721 tataagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg
    12781 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    12841 ccagccccca tggacaccac catcaaagcc aggaaagcgt gcatacgcgc cttcatgact
    12901 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    12961 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    13021 tcgatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac
    13081 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcgactttca
    13141 atcacatcaa aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc
    13201 aagtcgcgat agtgcttttt cagtgcagaa caggcgatca gcgacacttt attggtgcgc
    13261 tgcatagcaa acgcggagtc gttcagcgcc tgcaaccagg gtttgcgatc gtcgtcattc
    13321 agcggttcgc cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca
    13381 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgccc
    13441 gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    13501 ccacaataca agaattaaaa atgttacggg taactgttat cggcaacatt gtccagccgg
    13561 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    13621 ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    13681 gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc
    13741 tcacgcggcg tcagcacgct cgcaagtcgt ggctccatca cctgaccaat gtcatgaccg
    13801 tggaaaccgg caatcgccat atcgtcagga acttttaacc ccagacgctg acattcaaac
    13861 gccgcgccga ccgccagatc atcattagta cagaacacgc catccagctg cggatattcc
    13921 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    13981 acgctatatg gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    14041 atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc acgagcaata
    14101 atggcggtag tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg
    14161 cacggcgact ggctgtccat caactccacc accgggatcc ccgccacttc aatcatcttt
    14221 aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc cgtcgatatt ccaggagagc
    14281 atggattcga gacgttcttg ctccatttcc ggtttataac cgtagtgcgc cagcatggtc
    14341 tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg
    14401 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tagcgttaga gaggatatcg
    14461 ggcgcacgat tgggaatata gcccagttca tcaagagcgg cggcaatctt gccgcgtaga
    14521 gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct
    14581 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    14641 aagagataga ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg
    14701 cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg
    14761 gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa
    14821 ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca gtaacacttc
    14881 gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct
    14941 ggtcgaggct ttcacgccgt taatggtaac attgcctttt accacctgaa tccagacgcg
    15001 gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc aacgatacag
    15061 ttccatatcc tgatgcactt tcaacgaacc atctcgcgca tccggcgaga gcaccagctg
    15121 tttgccctgc acggcatcga agcgacgctg ttcataacgc ggcgtaatgc cattttcttc
    15181 aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga
    15241 gtgacgaata cccgtacccg cactcataat ctggaactca cccgccggaa cctgctcttt
    15301 attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca aaatttccat
    15361 atctttatgc gggtgagtgc cgaagccctg ccctacttca atcacgtcgt cgttaatcac
    15421 gcgcagcgcg gagaagccca taaagttcgg atcgtaatag ttggcacaag agaaagtatg
    15481 ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat
    15541 catttgtatt cgccccctga atgatttcga tgggcttagt gtggacccga tctgcctggg
    15601 atgatagagg gtgaaaattg ccccctctgt tcaaaaaaat tgaactattc gaggggccat
    15661 caaacttaat tcgcgagagt cactgtggag ggagaggctt gctcaaatcc gcgttcaagg
    15721 atttccagat tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg
    15781 gtttgataca acgcatcata aacacgcccg taatcgccca tctccggctt catctcttct
    15841 ctgaccgtca caccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg
    15901 aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg
    15961 tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc
    16021 agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg
    16081 tcgtcaggat tggctttatt acgcaggctg cggatgtcat aagcgacgtg atccgggcga
    16141 ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc
    16201 tgcggcagtc caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact
    16261 tcgacaatct ctcccagctt gccgctttca atcgcttttt tcgccgtcag gaagcaggaa
    16321 tcaaagcgac gattctgata tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac
    16381 agctctttcg cctgcgcaag tgtcggagtg aacgtttttt cgaccagcac atttttcccg
    16441 gcttccagcg cgcgcttcgc gtactcgaag tggctgtccg catgggtgca gacaacaacc
    16501 aacttaacat cgggatcgtt tagcacttcg tcgagatcgc tggtgaaatg gatatgagag
    16561 taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc gacatgccag
    16621 ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca
    16681 ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag
    16741 cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac tcccgtggat
    16801 aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa
    16861 gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgataca
    16921 ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc
    16981 gcatttgaaa gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag
    17041 tgagatagta atacgccacg cagaaacacg ggattacgag gccatcaggc agattcacgc
    17101 ccagccggag gtgtattgca acacactaca ggtgcctcat ccttccgatc atatgtggca
    17161 ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg atggagacgt
    17221 cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg ttgccgattt
    17281 tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga
    17341 gatgattgaa atgtgcgaca actggttacg ggtagatcgc attgaactaa ccgtgtttgt
    17401 cgataacgcg ccagcaatta aggtctataa aaaattcggc tttgagattg aagggaccgg
    17461 taagaagtac gcattgcgta atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa
    17521 atagagaatt tgcaatctaa gtttgtcatt taagccctca cctaccggga gggcttaaga
    17581 tattaatacc ccgccgttaa atcatccacc gagcgcgggt cggatgcgcc gtacaactca
    17641 ccgtccggcc caaccataat gctttgtgtg ctgcccatcg cctctttcag cgccactttc
    17701 tgaccttttg cttccagcag cttgagcgta tccgggctaa accctttttc gacacgcagc
    17761 tcgtccggca accactgatg gtggaaacgc ggcgcattgg tcgcttcggc gacgttcatg
    17821 ccataatcga tgctattcac caccatttgc agcaccgtgg tgataatccg gctaccgcct
    17881 gggctaccgg taaccagcca ggttttaccg tctttcacca caatggtcgg cgacatcgac
    17941 gacagcgggc gtttgttcgg cccgacggcg ttggcatcac cgcccaccag cccgtaaacg
    18001 ttcggtacgc ccggtttggc ggagaaatca tccatctggt tattaagcag aataccgctc
    18061 tcacccgcga caatacccgt accgaaggtg gtgttcagcg tataggtcac cgccaccgcg
    18121 ttaccgtctt tatccactac agagtaatgg gtagtttgat tactctcata aggcgcaagc
    18181 ttgccagggc gaatctcact ggacggcttc gcttttttga tatcaatttg atcggcaatg
    18241 gatttggcat aggctttatt ggtcagtgcc tgccacggga ctttgacaaa atccgggtcg
    18301 ccaagatatt ccgagcggtc ggcgtaggcg tatttctccg cttctgccat gatttgcatc
    18361 gcatcggcgc tgccaaagcc gtatttcttc atatcgaagt tttccagaat attgaggatt
    18421 tgtacgatat ggatcccgcc ggaggatggc ggtggcatgg agtaaacctg atacccgcga
    18481 taatcgccgc ttatcggagt gcgttcgacc gctttatagg ccgctaaatc ttctttagtg
    18541 atcaagccac cgtttttctg catctcctgg gcgatctgtt ccgcaatcgt gcctttatag
    18601 aattcgtccg ggccgttttc agcaatcatc tccaggctct ttgccaggtt cgcctgcacc
    18661 agcgtgtcgc cctttttcag cggctcgccc tctttccaga agatagcttt actgttttcg
    18721 tgattcggca acacttcgct accgtaggtt ttgagatcgt cagccagcgc gtcgttaacg
    18781 ataaaaccat cgcgtgccag tttaaacgcg ggctgcacga ctttgttcag cggcatggtg
    18841 ccgtatttat ccagcgccag cgagaaacct gctaccgtac ccggtgtgcc ggaagccaga
    18901 tgcgaagtga gtgatttttt gctgtccggg ttgccctgat catcgaggaa catatcgcgg
    18961 gtcgctttgg cgggtgccat ttcgcggaaa tcgatagccg tggtattgcc atttttcgag
    19021 cggattaaca taaaaccacc accgcccaga ttccctgcct gcggatgcgt taccgccagc
    19081 gcgtagccca ccgccacggc ggcatcaacg gcattcccgc cctccttgag gatatccacc
    19141 cccacctgag tggcagtggc gtccacagac gctaccattc cctgtttcgc gcgtaccgga
    19201 tggaagacat cttcctccac accatacgag acgggcggcg caggaggcgc ggcggcggcg
    19261 ctaaaacaac ttcctgagag cagagcagca atggccaccc ggcgtaaaaa cgtcggtttt
    19321 atcatcgtta ttctccagag attaaggggc aaccccagct aagcctggta tataactctg
    19381 aattaatcat cgttttgtcg ggaagcgagt aaacttaaag gatatcttca gaggaggata
    19441 cgcgatgaaa cgacttctga ttcttacggc actcctgccg tttgtcggct ttgcacagcc
    19501 cattaatact ctgaacaacc ctaaccagcc ggggtatcag atacccagcc agcagcggat
    19561 gcaaacccag atgcagactc agcaaatcca gcaaaaaggg atgctgaatc agcaactgaa
    19621 aacgcaaact cagttgcaac agcagcattt agaaaaccag ataaacaata attctcagcg
    19681 ggtgttgcag tcacagccgg gggagcgaaa tcccgcccgg cagcaaatgc tgcccaacac
    19741 caacggcggg atgttaaaca gcaaccgtaa tccggatagt tcgttgaatc agcagcatat
    19801 gctgccggag aggagaaaca gcgacatgct gaatcagccc agcacgccgc agcctgacat
    19861 tccgttgaaa actattgggc cgtaaagttc ggaccaatca cgtcaatcgc atcggtacag
    19921 atgcaatcca cgccccagcg cagcaactct gccgcgtgct ggggtttatt gacggtataa
    19981 accagaatcc gtaatccggc gtctttcaac tgcatcactc gcgctttatc gagtaactta
    20041 tgattgagat gaatagagac gcagcccagc cgcgcggtca gttcgcgcca gtcgtcgcgc
    20101 cactcatcaa gcaataaacc acgcggcagt tccggtgccg cctgttgtgc agcttctaaa
    20161 gcatcaatct caaacgatga cagcagcggc ggcgtcatac cggcccacag ttggcgtgcc
    20221 gccagcgcca ccattttgcc cgttaatggt ccagtgccag tggtgggttt gatttcgata
    20281 ttcgccatca tcccgtgttc gcgacagcgt tccgccacct gcgaaagcaa cggcagcggc
    20341 tcacctttaa acgctttgct gtaccaactg cccgcatcca cgcgcagtaa atcctgccag
    20401 ttcagttcac ccgcgacgcc ccagccgttg ctggtgcgtt cgagattgtc gtcatggagc
    20461 aggaagatct cgccatcttt cgataacttc gcgtcaaatt cgatcatctt atgaccgtat
    20521 tttgccccga cgtcgattgc cgccagggtg ttttccgggg ccagcttacc gccgccacga
    20581 tgagcgacga tgcggggata aggccagtta ctcatactcg ttgtcctgtt tcaccatcaa
    20641 aaagatgcag ctgattttcc gccagatgca gccacagcgt gctgcctgcc gtcgggcgct
    20701 cctgatgcgc cagtcgcacc accagcttct gcttgcgagc ttagcgcaat atgttccggg
    20761 cgaatgccga gggtcatttt acgcccggca tactgacggt agccaccgtt tagcggtagc
    20821 acgataccgc cgtccagttc gaaatgcgtg ccttcattat tcatgcggcc tgtcagcagg
    20881 ttcatcgccg ggctgccgat aaaactcgcc acaaacaggc tggcgggctt ttcgtagact
    20941 tccaccggcg tgccaatctg ttcggcaacg ccgccgttca tcaccattac tcgctgggcg
    21001 agcgtcatcg cctcaacctg atcgtgagta acgtagagtg aagtcgtttt caggcgacgg
    21061 tgcagctgtt gcagttcaag acgcatctgt acgcgcagct tggcatcgag gttagagagt
    21121 ggctcatcaa acaagaacac cgccggatcg cgcacaatcg cgcgtcccat cgccacacgc
    21181 tggcgctgac cgccggaaag ctcgcgcggg cgacgtttga gcagaccgtc cagctccaga
    21241 atgcgcgccg cttctttaac gcgctcggct atttgctgct tgcccatgcc gcgaattttc
    21301 agcccccacg ccatgttttc ttcgacgctc atatgcggat aaagcgcgta attctggaac
    21361 accatcgcaa tcccgcgatc tttcggctcc atttcggtca cgcgctggtc gttgatccag
    21421 atatcgcctt cggtcaccct ctccagcccg gcaaccatac gcagcagcgt cgatttcccg
    21481 cagccagacg gcccgaccat cacgataaat tcgccatccg ccacatcaag ggtcagcggt
    21541 ttaatgacct aggttttgcc atcccagctt ttggttactg cctgtaattt cagtcctgcc
    21601 atcttatttc tcactatcga ccaggccgcg cacgaaggca cgctgcatca ctaaaacaat
    21661 cactactggt gggatcaggg ttaacaacat cgccgccatc actgagttcc attccgtggt
    21721 gccttcgcca gtggcgatca tccctttgat ccctgccacg gtggtgccga gatccacgtc
    21781 ggtaataatc aacaacggcc acaaatactg gttccagccg taaataaagg tgatcacaaa
    21841 cagcgccgcc agattagttt tggagagcgg aaaaacgatg tcgcaaaaga agcgcattgg
    21901 tgacgcaccg tcgatccgcg ccgcttccac cagttcatcc ggcagcgtca taaagaactg
    21961 gcgaaacaga aacgtggcgg tcgccgaggc catcagcggc agcgttaaac cggcgtagct
    22021 gtcgagcatc ttcaggttgg cgatgacttc caccgtcggg aagatacgta cttcaaccgg
    22081 cagcatcagg gtgataaaaa tcatccagaa gaagaggtta cgtagcggaa aacgaaacca
    22141 gacaatggca aatgccgaga gcatcgagac agtaattttg ccgagcgtaa tgctgaacgc
    22201 catcacaaag ctgttaagca acatccgcca gaacggcgcg ctatttgtgc ctaccccgtt
    22261 cacccagatg ttgtggatgt tttccagcag atgcgtgccg gggatgagcg tcatcggcgc
    22321 ggcgtagacg gcctgtttat ccagcgtcgc cgcgacaaac gccacgtaca gcgggaagag
    22381 gatcaccgcg atcccgagga tcagcatggt atggctgaat atcgtcagcc acggacggtt
    22441 ctcaatcatt ggtaacgcac cttgctttca acatagcgga actgcaccac cgtcagcacg
    22501 atgacgagga acatcagcac catcgactgt gcggcagacg aagccagatc cagtccggta
    22561 aaaccttcgc ggtagatctt ataaatcagc gtcgtggtgg cctgaaccgg cccgccggac
    22621 gtggcggcgt cgatcaccgg gaaggtgtcg aagaaggcat acaccaggtt cactaccagc
    22681 aggaagaaac tcaccggggc gataagcggc agcgcaatct taaagaagcg gcgaatcggc
    22741 cctgcaccgt cgatggctgc ggcttcgatc aacgaacggg gaatggattg cagcgcggca
    22801 tagaagaaca gaaagttgta gctgatttgc ttccacaccg aggcaaacac caccagaaac
    22861 atcgcctgac cgctattttg cgcgtggttc cagtcgtagc cgaactcagc gagaaaatgg
    22921 gtgatcagcc cgcgaccggg gttaaacagg aagatccaca atacggcggc aacggcggga
    22981 gccacggcat acggcagcaa cattaaggtt tggtagaaac gactgccgcg cacgatgtac
    23041 tccaccagcg ccgcaaagaa cagcgatacc agcaaaccgc tgacggtgac aaaggtgctg
    23101 aattttatcg tcgtccagaa ggagtcgaga taatagctgt catgaaacag cgtgacgaag
    23161 ttatccagac cgacaaactg gctggagaaa ccaaacggat cgacgctttg tagcgagtac
    23221 cacaacgctt cgcccgcagg ccagataaaa aagataacgg tgatgatgag ctgcggcgcg
    23281 accagcagat aaggcagcca gcgcgagcgg aacaccggac gggatgatga cattacggtt
    23341 aattcctgaa ctgtgccgga tgcgcttcgc ttatccggcc tacacgcgat gccaggtcag
    23401 ataagcgaaa cgcatccggc attacactga ttaagacttc gtcgatttct caaagcggcg
    23461 cagtaactgg ttcccacgct caactgcggt atccagcgcc tgctgtggcg tcttcttacc
    23521 ggtccacacg ctctcaagct cttcatccac aatcacgcgg atctgcggca tgttgcccag
    23581 acgaagccct ttggtgaacg gcaacggcgg cttgttcagc atctgacgcg tcgcggtatc
    23641 cgcacctggg tttttctcgt aaaagccctg ctcacgggtc aggtcatacg ccgctttggt
    23701 aattggcaga taaccggttt tctgatgcca ctcagcagcg ttttctggct tcgcgaggaa
    23761 gtcgagaaac ttcgccacgc cggtatacgt ttctttatct ttgccctgca tcacccacag
    23821 gctggctccg ccgataatgg cgttttgtgg cgcatctttc gcatcggcgt cgtaaggcat
    23881 catacctacg ccgtagttaa atttggcgta ctcgcgaatg ttggcaagag aaccggaaga
    23941 ggcggtggtc atcgcgcaat caccgttata gaacttctcg gtggattcat ccttacgacc
    24001 gacgtagctg aagtcgccct tcttgttcat ctcctcgagc atggcgatgt gtttcacctg
    24061 ctccggcttg ttgaactcca gcaccgcgtc cgtgccgtca aagccgttgt ttttgctggc
    24121 aaacggcaga ccgttccagg cgctaaagtt ttccagttgg atccagccct gccagccgct
    24181 ggcgtagccg cacttaatac cggaggcttt cagttttgcg gaatagtccg ccagatcctg
    24241 ccaggttttc ggcggctgtt ccgggtctaa ccccgctttc ttgaaggcgt ctttgttgta
    24301 atagagaacg gaagtcgagc tgttgaacgg ctgggagagt aagtggccgg ttttgctgtc
    24361 ggagtagtaa ccggaaaccg tcggcacaaa ctgcgactca tcgaactgaa tccctgcctc
    24421 tttaaacacg tcatacaccg gtttaatggc tttcgacgcc atcatggtgg cggtgccaac
    24481 ttcataaacc tgcaaaatag ccggagcgtt gccggtacga aatacggcaa tccccgcgct
    24541 taaattctgt tcgtagttgc ctttataggt cggtacaatt ttgtaatccg gattttcggc
    24601 gttaaaacgt tgggccagag aatccacctc tttacccagt tccccttcca tagaatgcca
    24661 gaacggaatg gtcgtcactg cctgtgcatt ccccattaac gccagtccga gcgccagtgc
    24721 tgaagctgta taatgtaacg gtttcatcgt ttatctctct tgttgtaccg aatgcgcgaa
    24781 ttcacgcgtt ttatgctcgc ggggtaacat gacatgctcg aattacagaa aaataacttt
    24841 tttgttacat ttgtaagata gtaaggtgtc agaaagatga caaggcggtg acggcgtggg
    24901 tgagggaaaa tgggagatgg ggcacggata agcggaaaaa tagaaggtct gaatcaaact
    24961 ctacagatta ctcatcgttt catgccggat gcggcgtaaa cgccttatcc ggcctacaag
    25021 atcgtgcaaa ttcaatatat tgcaactcac ccagtaggcc tgataagcgc agcgcatcag
    25081 gcaattttgc atttgtcacc tgtctcaaag gagtcttttg actccttatc aatcaacgtg
    25141 ttattacccg cctaaatacg cacttctcac tgcttcgttc gccagcagcg cgtcgccggt
    25201 atcggagagc accacatggc cgttttccag cacgtagccg cgatccgcca gctttagcgc
    25261 ctggttggcg ttctgctcga cgaggaagat agtcatcccc tgctcgcgca gctgctcgat
    25321 ggtgtcgaaa atttgctgga tgataatcgg cgcaagaccg agcgatggct catcaagtaa
    25381 caacaaacgc ggattgctca tcagcgcgcg accaatcgcc agcatctgct gttcaccgcc
    25441 ggacatggtg cccgcccgct gaacacggcg ctcatgcaga cgtggaaaca gttcatacac
    25501 ccactttatg cgctcctgaa actggtcacg ttcggcaaag aagccgccca tcgccaggtt
    25561 ctcttccacc gtcatccgcg agaagacgcg acgcccttcc gggacaatcg ctaccgcttc
    25621 gcgcatgatt ttcgccgtct gccagtcggt aatgtcttta tcatcaaaca caattcgccc
    25681 gctggtggcg cgcggatcgc cgcataacgt gccgagcaag gtggttttcc ccgccccgtt
    25741 cgcgccaatg agagtgacaa tctcgccctg attgatatgc aggctcacct catgcagcgc
    25801 ctggattttg ccgtagtggg cgctgacttt gtcaaaggac aacatgactt tttccatctt
    25861 atgcctcacc taaataggca cggatcacgt ccgggttatt acggatttgc tccggcgtac
    25921 cgtttgccag tggcgtcccc tgattgacca cataaattcg gtctgaaatc cccatcacca
    25981 gcttcatatc gtgttcaatc aacaaaatag tggtgttgtg atgattgcgc agttcggcaa
    26041 tcagctcatc cagctctttg gtttcttttg ggttaagacc tgccgccggt tcgtcgagca
    26101 ttaaaatctc cggctgcgtc accatgcagc gggcaatctc cagacggcgt tggtcaccat
    26161 aggccaggtt actcgcctga cggttggcgt gttccagcaa accaatgcgc tcaagccagg
    26221 tggcggcgcg gtcgagcgct tcgctctggg cgcgacggaa agaaggcgtt ttcaacaggc
    26281 cagagaacag cccggttttc agttgctgat gctgcgccac cagcaggttt tcaattaccg
    26341 tcatttcacg gaacagacgc acatgctgga aggtgcgcac cacgcccatg cgagcaattt
    26401 gctgccccgg taaaccttca agatgctgat cgcgcagtaa aatggtgccg ccggtgggtt
    26461 tgtagaaacc ggtcaggcag ttaaaaaccg tggtttttcc ggcaccgtta gggccgatta
    26521 acgagacgat ttcctgcggg tacagttcaa gattgacgtt gttcaccgcc agcaggccgc
    26581 cgaagcgcat catcagaccg ttaacagata ataatggctg actcatgcct gctctccttt
    26641 cgctgcgccg tttttcagct tcagttgcgg gcgcgtcatg ggcagcaaac cctgcggacg
    26701 ccagatcatc atcagcacca tcaaaccacc gagcattaac atgctgtatt cgttgaaatc
    26761 acgcatcaat tcgcgcgaca ccaccagcag aacagccgcc agaatcactg caaattgcga
    26821 gcccataccg ccgagcacca ctatcgccag cacaaaggcc gattcagcaa aggtgaagga
    26881 ttccgggctg acaaagccct gacgcgccgc aaacagcgtt ccggcaaaac cggcaaatgc
    26941 ggcgctgatg gtaaaagcag tcagcttgat acggcgcggg cttaagccca gcgaacgaca
    27001 ggcgatctcg tcttcacgca acgcttccca cgcgcggccc agcggcatcc gcagcaggcg
    27061 gttaatgaca aacaggctta acaccaccag cagcaacgcc accaggtaga ggaagataac
    27121 gcgatcggac ggatcgtatt tcaggccaaa gaaattactg aacgtgtccc agccgccttc
    27181 acgagcggta cggctgaact cgagtccgaa gagtgtcggt ttcgggatct gactgattcc
    27241 gttcgggccg ccggtgattt cagtgttatt gagcagcaat atgcgcacaa tttcgccgaa
    27301 accaagggta acgatcgcca gatagtcacc gcgcagacgc agcaccggaa aaccgagcag
    27361 gaaacccgcc gccgctgcca tcagtccggc aatcggcaaa caggtccaga accccagacc
    27421 gtaatagtga ttgagcagcg caaaagtgta agcgccgatg gcgtaaaaac cgccgtaccc
    27481 cagcaccagc agaccagaaa gaccaacaac cacattcagc ccgagaccga ggataatgta
    27541 gatcatggtc agagtggcga tatccaccgt cccacgtgac accataaacg gccacgccac
    27601 cacaagcacc agcagcgcca cgaggaacag cttttgcttc accgtggacc catcaatcgc
    27661 gggcagaata aacttcggcc cggaaacgct tttcaaccct ttctggaaag ccggtcgcaa
    27721 aagctggaag aaaaagacca ccgccgtgcc gataaacatc cactgccaac ggatgtctga
    27781 agccgtgtcg accaccagtt tggtgccatc cagctccagt tgtacgccca taaagacgcc
    27841 cgccaggaca aagaacatcg cggcagagag cagtgccatt gcaatatgca tcggtttcat
    27901 actttctcta cctccgggcg acccagaata ccggtcggca tcaccagcag caccagaatc
    27961 agcagggcga atgagaccac atctttatat tccgtactca gataggcaga agagagcgcc
    28021 tccgcaatcc ccaggatcag cccgccaatc atcgcccctg gaatgctgcc aatcccaccg
    28081 agcaccgcag cggtaaaggc tttcatcccg gccataaagc cgatataggg gttaatgacg
    28141 cagtagaact gaccgagcag tacgccagcc actgccgcca tcgccgcgcc aataacaaag
    28201 gtcaaagcaa tcacccggtc ggtgttgatg ccaagcaggc tcgccatttt cagatcttcc
    28261 gcgcaggcac gacaggcgcg ccccatacgg gaatagcgaa tgaaaatcgt cagcgccagc
    28321 atggcgagga aggtgacaat ccagatcacc gcctgcatgg tggtaataga ggcagagaag
    28381 ttttcgctat ggcccaccac ccactgaccg ttaaacaggc tcggcagcgc cacgtcgcgc
    28441 gaaccttcgg tcaggctgac gtagttttgc aggaagatgg acataccgat tgcggaaata
    28501 agcgcaatca ggcgcttaga gttacgcacc gggcggtaag ctacccgttc gatactccag
    28561 ccgtaggcgc tggcgatgac gattgcgccg acgaatcccg ccgccaccag cagccagccg
    28621 gtatcaatgc ccatcatcat cagcgcggcg atgatcataa atgagacgta gctgccaatc
    28681 atataaacct cgccgtgggc gaagttgatc atgccgataa tgccgtaaac catggtgtag
    28741 ccgatggcta tcagcgcgta ggtactgccc agcgtgacgc cgttaaacat ctgctgcaag
    28801 aaatacaaaa actgctcaga cataaggtaa cctttctaaa cccgcccgca ttttacgggc
    28861 ggtgggatga tcacttggct gccgtggatg aaccgtcggc gtgccactgg aagacaccaa
    28921 aatcaaatcc cttaagatcg cctttttcat cccagttcag cggcccaatc acggtgtttg
    28981 caccgttagc ttttaaatct ttcaccagcg ccagcggctc atcgctgccg gtacgctcaa
    29041 gggcagttgc cagagattgc actgccgcgt aagtgatcca gacataaggc ccggacggat
    29101 ctttcttgtc tgctttcagc gcatcaacga tgccctggtt tgccggatcc tggtcatagc
    29161 gttttggcat agtgaccaac atgccttcgg cggcatcacc ggcaatgttc gacagcgatg
    29221 cgttaccgac accttccggc cccataaact gggttttcag gccaacggaa cgggcttggc
    29281 gcagcatctg ccccatttcc gggtagtaac cgccgtagta aacgaagtcg atattttctt
    29341 ttttcaggcg ggcgatcagc gcggagaaat ctttctcccc ggcggtaata ccgtcgaaga
    29401 agacgacgtt ggcgttagcc gctttcagcc cgtcctgcac cgaacgcgcc agcccttcgc
    29461 catactgttg tttgtcgtga atgatggcga tgcgctgggg cttcaccgtc tcaagaatgt
    29521 attttgccgc cgtaggcccc tgggaagagt ccagcccggc agtacgcata atgtgttgat
    29581 aaccgcgttg ggtcagctcc gggttggtcg ctcccggcga gatcatcaga ataccttcgt
    29641 cttcgtagat atccgacgct ggctgggtag aggaagaaca cagatgacca ataacgtatt
    29701 taatgccgtc gttaacgatt ttgttggcga ccgcaacggc ttgtttcggg tcgcaggcgt
    29761 cgtcatattc cacgccaacc agcttgtcgc ccttaattcc ccctttgaca ttaatgtctt
    29821 taatcgcctg acgcgcgccg ttaaattcca tatcgcccca ctgggcaatc gggccggaca
    29881 tcgcgccgac aacggcgact ttaatatcgt cagccatagc ggtgtgtgaa attgccagtg
    29941 caatcatccc tgcgatgata gttttcgcat tccgtttcat agtcaaaaat ccccattcgt
    30001 gatgttgtgt tgctttgttt ttatgtgtta acaaatcaga ctgttctttt tttatactgc
    30061 actgtttttg cctgtctgat taaggggtta gcgcagtatt ttgtgatgat agcgattaaa
    30121 atccctattt ttcagtcgat taagaacaga taatattctg aatttattga tagataaaca
    30181 gaaaaaagtg cctttgtcag cataaaataa cggcacaaag ggcg
//
LOCUS       DAZOYF010000033        29727 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZOYF010000033 DAZOYF010000000
VERSION     DAZOYF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.033"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..1224
                     /locus_tag="ACHUOF_001406"
     CDS             <1..1224
                     /locus_tag="ACHUOF_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR0610654.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="glpX"
                     /locus_tag="ACHUOF_001407"
     CDS             1221..2186
                     /gene="glpX"
                     /locus_tag="ACHUOF_001407"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR0610655.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="ACHUOF_001408"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="ACHUOF_001408"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFR0610656.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="ACHUOF_001409"
     CDS             2714..3427
                     /locus_tag="ACHUOF_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR0610657.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="ACHUOF_001410"
     CDS             complement(3399..4076)
                     /locus_tag="ACHUOF_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0610658.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="ACHUOF_001411"
     CDS             complement(4070..4747)
                     /locus_tag="ACHUOF_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0610659.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5442)
                     /locus_tag="ACHUOF_001412"
                     /pseudo
     CDS             complement(4735..5442)
                     /locus_tag="ACHUOF_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(5443..6021)
                     /locus_tag="ACHUOF_001413"
     CDS             complement(5443..6021)
                     /locus_tag="ACHUOF_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR0610660.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="ACHUOF_001414"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="ACHUOF_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="ACHUOF_001415"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="ACHUOF_001415"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR0610661.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="ACHUOF_001416"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="ACHUOF_001416"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR0610662.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHUOF_001417"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHUOF_001417"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR0610663.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="ACHUOF_001418"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="ACHUOF_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR0610664.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="ACHUOF_001419"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="ACHUOF_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR0610665.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="ACHUOF_001420"
     CDS             12291..13031
                     /locus_tag="ACHUOF_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR0610666.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="ACHUOF_001421"
     CDS             13198..14148
                     /locus_tag="ACHUOF_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0610667.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHUOF_001422"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHUOF_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHUOF_001423"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHUOF_001423"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR0610668.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHUOF_001424"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHUOF_001424"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR0610669.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHUOF_001425"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHUOF_001425"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR0610670.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHUOF_001426"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHUOF_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR0610671.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHUOF_001427"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHUOF_001427"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR0610672.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="ACHUOF_001428"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="ACHUOF_001428"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR0610673.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHUOF_001429"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHUOF_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR0610674.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="ACHUOF_001430"
     CDS             23768..23827
                     /locus_tag="ACHUOF_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0610675.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="ACHUOF_001431"
     CDS             complement(23928..24530)
                     /locus_tag="ACHUOF_001431"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR0610676.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="ACHUOF_001432"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHUOF_001432"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHUOF_001433"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHUOF_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR0610677.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHUOF_001434"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHUOF_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR0610678.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="ACHUOF_001435"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="ACHUOF_001435"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR0610679.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHUOF_001436"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHUOF_001436"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR0610680.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHUOF_001437"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHUOF_001437"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR0610681.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
BASE COUNT         7098 a         7436 c         8024 g         7169 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg actgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaata
//
LOCUS       DAZOYF010000034        28914 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZOYF010000034 DAZOYF010000000
VERSION     DAZOYF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.034"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHUOF_001438"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHUOF_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR0610682.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="ACHUOF_001439"
     CDS             complement(2138..2584)
                     /locus_tag="ACHUOF_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR0610683.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="ACHUOF_001440"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="ACHUOF_001440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR0610684.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHUOF_001441"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHUOF_001441"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR0610685.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="ACHUOF_001442"
     CDS             complement(5347..6009)
                     /locus_tag="ACHUOF_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR0610686.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="ACHUOF_001443"
     CDS             6182..6595
                     /locus_tag="ACHUOF_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR0610687.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHUOF_001444"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHUOF_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR0610688.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHUOF_001445"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHUOF_001445"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFR0610689.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="ACHUOF_001446"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="ACHUOF_001446"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR0610690.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHUOF_001447"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHUOF_001447"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR0610691.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHUOF_001448"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHUOF_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR0610692.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="ACHUOF_001449"
     tRNA            complement(9861..9948)
                     /locus_tag="ACHUOF_001449"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHUOF_001450"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHUOF_001450"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR0610693.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHUOF_001451"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHUOF_001451"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR0610694.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHUOF_001452"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHUOF_001452"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFR0610695.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHUOF_001453"
     CDS             14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHUOF_001453"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR0610696.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHUOF_001454"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHUOF_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR0610697.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="ACHUOF_001455"
     CDS             14984..15841
                     /locus_tag="ACHUOF_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR0610698.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="ACHUOF_001456"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="ACHUOF_001456"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR0610699.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="ACHUOF_001457"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="ACHUOF_001457"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0610700.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHUOF_001458"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHUOF_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR0610701.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="ACHUOF_001459"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="ACHUOF_001459"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR0610702.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHUOF_001460"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHUOF_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHUOF_001461"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHUOF_001461"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR0610703.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="ACHUOF_001462"
     CDS             complement(22884..24023)
                     /locus_tag="ACHUOF_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR0610704.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="ACHUOF_001463"
     CDS             complement(24069..26165)
                     /locus_tag="ACHUOF_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0610705.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="ACHUOF_001464"
     CDS             complement(26165..26911)
                     /locus_tag="ACHUOF_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0610706.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHUOF_001465"
     CDS             complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHUOF_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR0610707.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="ACHUOF_001466"
     CDS             complement(27659..27964)
                     /locus_tag="ACHUOF_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610708.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="ACHUOF_001467"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="ACHUOF_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR0610709.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7003 a         7320 c         7600 g         6991 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataa
//
LOCUS       DAZOYF010000035        28670 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZOYF010000035 DAZOYF010000000
VERSION     DAZOYF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.035"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            164..649
                     /locus_tag="ACHUOF_001468"
     CDS             164..649
                     /locus_tag="ACHUOF_001468"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610710.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPETKFTLLNVT
                     K"
     gene            646..1032
                     /locus_tag="ACHUOF_001469"
     CDS             646..1032
                     /locus_tag="ACHUOF_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610711.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1057..3270
                     /locus_tag="ACHUOF_001470"
     CDS             1057..3270
                     /locus_tag="ACHUOF_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFR0610712.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3293..3738
                     /locus_tag="ACHUOF_001471"
                     /pseudo
     CDS             3293..3738
                     /locus_tag="ACHUOF_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3760..7318
                     /locus_tag="ACHUOF_001472"
                     /pseudo
     CDS             3760..7318
                     /locus_tag="ACHUOF_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459289.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            7538..7741
                     /locus_tag="ACHUOF_001473"
     CDS             7538..7741
                     /locus_tag="ACHUOF_001473"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610713.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7759..8079
                     /locus_tag="ACHUOF_001474"
     CDS             7759..8079
                     /locus_tag="ACHUOF_001474"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610714.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8145..8377)
                     /locus_tag="ACHUOF_001475"
                     /pseudo
     CDS             complement(8145..8377)
                     /locus_tag="ACHUOF_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8487..9257)
                     /gene="yafV"
                     /locus_tag="ACHUOF_001476"
     CDS             complement(8487..9257)
                     /gene="yafV"
                     /locus_tag="ACHUOF_001476"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR0610715.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9438..9884
                     /gene="ivy"
                     /locus_tag="ACHUOF_001477"
     CDS             9438..9884
                     /gene="ivy"
                     /locus_tag="ACHUOF_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR0610716.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9927..12371)
                     /gene="fadE"
                     /locus_tag="ACHUOF_001478"
     CDS             complement(9927..12371)
                     /gene="fadE"
                     /locus_tag="ACHUOF_001478"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR0610717.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12611..13189
                     /gene="lpcA"
                     /locus_tag="ACHUOF_001479"
     CDS             12611..13189
                     /gene="lpcA"
                     /locus_tag="ACHUOF_001479"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR0610718.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13395..14162
                     /locus_tag="ACHUOF_001480"
     CDS             13395..14162
                     /locus_tag="ACHUOF_001480"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR0610719.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14133..14873)
                     /gene="dpaA"
                     /locus_tag="ACHUOF_001481"
     CDS             complement(14133..14873)
                     /gene="dpaA"
                     /locus_tag="ACHUOF_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR0610720.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15257..15778
                     /locus_tag="ACHUOF_001482"
                     /pseudo
     CDS             15257..15778
                     /locus_tag="ACHUOF_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15954..16451
                     /gene="rayT"
                     /locus_tag="ACHUOF_001483"
     CDS             15954..16451
                     /gene="rayT"
                     /locus_tag="ACHUOF_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR0610721.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16668..18380)
                     /locus_tag="ACHUOF_001484"
                     /pseudo
     CDS             complement(16668..18380)
                     /locus_tag="ACHUOF_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18352..19137
                     /gene="lafU"
                     /locus_tag="ACHUOF_001485"
     CDS             18352..19137
                     /gene="lafU"
                     /locus_tag="ACHUOF_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR0610722.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19208..20263
                     /gene="dinB"
                     /locus_tag="ACHUOF_001486"
     CDS             19208..20263
                     /gene="dinB"
                     /locus_tag="ACHUOF_001486"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR0610723.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRECRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20315..20608
                     /gene="yafN"
                     /locus_tag="ACHUOF_001487"
     CDS             20315..20608
                     /gene="yafN"
                     /locus_tag="ACHUOF_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFR0610724.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20611..21009
                     /gene="yafO"
                     /locus_tag="ACHUOF_001488"
     CDS             20611..21009
                     /gene="yafO"
                     /locus_tag="ACHUOF_001488"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFR0610725.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21019..21471
                     /locus_tag="ACHUOF_001489"
     CDS             21019..21471
                     /locus_tag="ACHUOF_001489"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0610726.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21717..22760
                     /locus_tag="ACHUOF_001490"
     CDS             21717..22760
                     /locus_tag="ACHUOF_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR0610727.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22822..23436
                     /gene="prfH"
                     /locus_tag="ACHUOF_001491"
                     /pseudo
     CDS             22822..23436
                     /gene="prfH"
                     /locus_tag="ACHUOF_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23493..24950)
                     /gene="pepD"
                     /locus_tag="ACHUOF_001492"
     CDS             complement(23493..24950)
                     /gene="pepD"
                     /locus_tag="ACHUOF_001492"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0610728.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25212..25670
                     /gene="gpt"
                     /locus_tag="ACHUOF_001493"
     CDS             25212..25670
                     /gene="gpt"
                     /locus_tag="ACHUOF_001493"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0610729.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25762..27006
                     /gene="frsA"
                     /locus_tag="ACHUOF_001494"
     CDS             25762..27006
                     /gene="frsA"
                     /locus_tag="ACHUOF_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0610730.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27064..27465
                     /gene="crl"
                     /locus_tag="ACHUOF_001495"
     CDS             27064..27465
                     /gene="crl"
                     /locus_tag="ACHUOF_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0610731.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27504..28559)
                     /gene="phoE"
                     /locus_tag="ACHUOF_001496"
     CDS             complement(27504..28559)
                     /gene="phoE"
                     /locus_tag="ACHUOF_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR0610732.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7083 a         7122 c         7606 g         6859 t
ORIGIN      
        1 aacaaatatt gatggcaata tcattataca taaggatggt aggaggacgt atttattaaa
       61 tcaaaatact ggttcgatta cggctgtgat aagatggaag tatgagtagt ataaggataa
      121 taccgttaca gatgactgga ctatatggga aaaagctatt tttatgcgag cgatatattt
      181 atctgttcag caggcatgga atgggaaaat aacttattct gtttccggtg aatctgagtt
      241 cgctaaaaaa tttcagggta aagctttgcc ttttgatgta agaataattt ctgcatctca
      301 gaatgaagac tggttggtta ttgcaacaaa ggttttgcct ggtgctgatt taagaacata
      361 tgttgatttt aaaaacagta cggttcatgt tgattcagcg ggcctggaaa aagtagcgaa
      421 atgtattaac tgtaataata cgttgcaagt aaatatacct catgaagccg gacatgtact
      481 tggttactta gatgatgatt atgattcttc gtctccgtat gttggagata ttagtgggtt
      541 gatgaatgtg ggaatggagc tttgggaacg ataccttaaa aacgcaacta taacgttgaa
      601 tatcataatg cctgaaacta aatttacttt attgaatgta accaaatgaa atttttctgt
      661 attatagtga ttttcctttc aataaattac atagctatag tgaatgtttt caagtcttct
      721 gcagttgctc aatccaccaa atattatgaa gaaaatgata tacatgatgg aagtaatgac
      781 atctttttta tgaagaaata tgggccgcct gctcatgagt tcgtgaggcc acgatataaa
      841 tttgttggtg agtattatat cggcttgaat caaacttatc gcataacaga tcctgaattt
      901 caagatgttc ctattaagga aatgttctgg aatttgcagg gggatttgaa tctgacatgt
      961 tggtttcatt ataaagacgg gcaatggaga gtgattactt atattttttg gccaccaggg
     1021 gctagatttt aatccggtat taaaggagca aatatcatga gttttgtatc tacgggtaat
     1081 aaaccagttg gaaacggtgg acccgtcatc accacaccac caatagctgg cgaaagtggc
     1141 ggtatgagca caggttcagc tgtaaccgat gtatctggtg ccgccgagga gatggcagaa
     1201 caggctgcgg cggacctgtt tggtgcgctg ccggaaccgt caggtctggt taaagccgca
     1261 gtagctgctg cgcaggctgc cgctgcagca gcagggatct cggatatggc tggtgcagtt
     1321 caggatgcag cggcgtcgct tgcagccggt gctccggggg cgcataacgt cacagtaagt
     1381 ggtagtgcgg tgccgccgca gatgcttttg tttgcaggca tgaatggttc ggaagggctt
     1441 gggaatctgt tttcttacac agtacagctt aaaacgctgg acgccctgaa cctggggtac
     1501 gtttcccccg ctgctaacct gccgcttcag ctcatggtgg gcaaagacct gtgcgtcagt
     1561 atcgaactgg acggcggcgg aaaacggtat atcagcgggc tggtgacggc ggcgagggtg
     1621 gtcggccatg agagccgttc ggtaacgtat gaattacgca ttgagccatg gctgaagctg
     1681 ctgacccata ccagcgacta caaagcgttc cagaacaaaa ccgtggtgga tattctcgat
     1741 gaagtactgg atgaatattc ctttccggtg gaaaagcggc tggtggaaaa ttatccaccc
     1801 cgcgcctggc aggtgcagta tggtgaaacc gacttcgatt tcatccagcg cctgatgcag
     1861 gaatggggga tttactggtg gtttgagcac agcgagaaca gccacacgct ggtgctggtg
     1921 gatgccatca atggtcataa agcgtgccct gactcgccgc tggtggagtg gcaccaggaa
     1981 gggctgaaac tggataagga atttatccac accattacgg ccagtgagag gctgcgtact
     2041 ggaaagtggg tgatggacga ttttgatttt atgaagcctc gttcgctgct taaaagtacc
     2101 gtggccagtc cgcgtgatac cggtcatgct gaatacgagc attacgaatg gcctggtgat
     2161 tacttcacta cgggcgaagg cgagatgctg acgcgcattc gtatggaagc gcagcgcagc
     2221 ccaggcagcc gggcacatgg ggccggtcat atccgtaccc tcatgaccgg gtacaccttt
     2281 acgttgatga atcacccgac ggcagaaatt aatcaggaat atctgctggt acagaccact
     2341 ctgtttttac gggataatgc gcagcacagc ggtcaggatc agcattttac ctatgtcacc
     2401 acgtttgaac tgcaccccac cagcgaagtt taccgcccac aacgaaccct gagcaaaccc
     2461 cacaccaaag gcccgcagag cgccatcgtc accggcccgg cggggcagga aatctggacg
     2521 gataaatatg gccgggtgaa ggtgcagttt ggctgggacc gttacggaaa gaatgacgaa
     2581 aacagttcct gctggatccg cgtcagctat ccgtgggcgg gtaaaggatt cggcatgatc
     2641 cagatcccgc gtattggcca ggaagtactg gtggatttca aaaacggcga tccggatttg
     2701 cccatcatcg tggggcgcac ctataaccag gacaccatgc cgccgtgggg gctgccgggc
     2761 atggcgtcgc agagtgggat attcagccac tcattgcagg gagggccgac taacggaaat
     2821 atgctgcgct tcgatgataa aacgggtgcg gaagaggtga agttccatgc ggaaaaagac
     2881 ctcaatacca cggtaaaaaa caatgaaacc cacacggtca tggtcgatcg cataaaaacc
     2941 attgtcaaaa atgaaaccat cagcgtggga gaagaccgta atgccaccat cacaaaaaat
     3001 gatggtcttt ccgtcaaact ggctcaaacc atcaatgtcg gaacgacata tcgtctggat
     3061 gttggcgatc aatttaccct tcgttgcggc aatgcagcgc ttgtattaca caaggatggc
     3121 tcgattgagt tttgcggaaa acaactgtta ctgcacacca gcgatgttat gcaactcatt
     3181 ggcagtggca ttgatatgaa tccggatggc ggcacagcca tgactgcgga tgatattgcc
     3241 ccgttaccta cccccgacgt ttctgaataa aaaatataaa aggaattttt ctatgaccag
     3301 cctttaccgt attcaggagg gctgctttgc cctgcctgaa acttttcttg atcgcaccgt
     3361 caatatcttc gtcccatcgg gcaatgaaag ggcaactccc agtctgaata ttttccgcga
     3421 tacgctcagg ccagatgaaa atctgaccac ctatattgac cgccagatag ccttgatgaa
     3481 aaaaaaaacc taggccagca ccgggtactt tcgcggggaa cggcactggc gggaactggc
     3541 gaagctgcca tgacaggtga acagattctc gcaactcatc gttctggaaa aacagaagtg
     3601 taccagcgtc aggccgggtt tatcaccggg ccagcaaagg tactaatgtt gacgctgacc
     3661 actcagcgtc cttttgatga tcacaccgac cagctatgga ctgcatggtt gactagtttt
     3721 cagcctgcta aaagctaaaa aaaacaggga ggttttgtca tgtatgaagc cgcacgggtg
     3781 gatgacccca tctaccacac cagcgccctt gccgggtttt tgataggggc catcataggt
     3841 atcgccatta ttgccgtcgc cagctttgct ttttttacct gcggcttttt ggccgggctg
     3901 attttgagtt ttctggctga tcagatagct tccggagtcc tgcaactggg tgaggctatt
     3961 gggcgctcga tacattccac agtgggaaaa attctgaccg gttctgataa tgtcagcacc
     4021 aacagtcgcc cggccgcccg ggccgtactg agtacggtga tatgcgaaga ccattccccg
     4081 gaaatccgca ttgcccaagg ttccggcaac atctacatca acagccagct gcccgtaagg
     4141 atgaccacac tgagtgtgat gcagtgattg aggacggctc ccccaatgtc tttctcggcg
     4201 gcggtacgca gacggtactg gaaatcagcc cggaaatccc tgactggctg cgtcaggtgg
     4261 tggatgtgct gtatgtggtc gccggaatgc ttggcgggct ggctggcgcc tggcgtcagg
     4321 cggcaaagat ggggtcgaaa tttggtacaa aatgtgcggc aaaatttatt ggtggcgaaa
     4381 tggtcgggat ggccatctct gacacagtca tgggattgtt cagcaatccg gtagatgtca
     4441 ccactgggca aaaaattttg ctgccggaga cggattttac cctgccagga cgcctgcctg
     4501 tcacctgttc gcgcttttac gccagccacc tggaaactga aggattgctg ggcagaggat
     4561 ggcggctgaa ctgggagata aacctgcgtg aggatgaaac atatatcacc ttcatcggtg
     4621 tacagggtcg ggaactgagt tatccgaaag agatgctgat accgggccac cagatattcg
     4681 acccggaaga gcagttttac ctgagccgtc tgcatgacgg atgctatgtg ctgcattaca
     4741 ccgactgcag ctattacgtg tttgatgaat ttgatgacca cggcgtcgcg ccgttacttt
     4801 ttatggagac gccgtaccgg cagcgtattg ctttcgggcg tgagaacggc aggctggtgc
     4861 gggtggcctc cagcagcgga caccatctgt tattgcaccg cacgatgacg caagcagggg
     4921 aacgcctgtc gcatattgaa cttttgaagg gcgggcgtcc cggtaacctg gtggaatatc
     4981 gttacgacga taacgggcaa ctgactggcg tggtgaaccg ggctggtgtg acggtgcgcc
     5041 agtttgccta tgaaaatggc ctgatgacag agcatcgcaa cgcgacgggc tttacctgta
     5101 cctaccactg ggaagagatt gaaggtttcc cgcgcgtggt ggaacacacg accagcgacg
     5161 gcgaggatta ccggttccac tatgattttg ctggcgggca gacggtggta accggcaggc
     5221 cggagcagaa gtggcagtgg tggtttgatg aagagacgta tgtgactgcg caccggacgc
     5281 cgggcggcgg aatgtaccgc ttcacgtaca acgaaaacca ttttccggta gcggtggaac
     5341 tccccggcga acgcagggta acgctggaat acgacaccct gagccgggtg gtgaaagaga
     5401 cggacccggc aggccgggtg acacagacgc agtggaacgg cagttttgct gaaatcacgc
     5461 gccgggcgct ggatgacgac cacgtatgga aggcggatta caacgaacac ggccaggtga
     5521 tacgggagac ggacccggaa gggcgcgtca cgcggtacgg gtatgatgat caggggctgc
     5581 cggagacggt ctgccacccc ggtaaacagc aggaccgtta cacctggaac gcgctggggt
     5641 tactgagcag ccaccgtcgc atcacgggca gtgtgcagtc ctggcagtat acgcagcgcg
     5701 ggatgctggc gcgccatacg gatgaagaga aacgggagac gcgctggcag tacaccccgg
     5761 aagggctggt tgccagtctg agcaacggca acggggctca gtaccggttc agctacgacg
     5821 gcgacggacg gctgacggga gaacagcgcc cggacggcct gataaggatg tttgcgctga
     5881 atgcggacgg gtttccggtc atcatcccga cgcagggcac agagggcggg gtgcgcaatg
     5941 aacagcagga gcgtgatgcg ctgggccgcc tgctgcgcag tgacacgcag cacagtacac
     6001 ggaccttcag ctacaaccgt cttgaccaga taacggaagt gacgctcacc cccacggaag
     6061 aaggggaacg gctgcaccac atgcaggcgg acacggtgcg ttttgcgtat gaccgcagcg
     6121 ggtggctgac ggcagagcac agcgtgcacg gcagtatcaa ataccggcgc gatgcgctgg
     6181 gcaatcccac cgatatcacg ttgccggacg ggcagcatct cagtcatctt tactacggca
     6241 gcgggcatct gctccagacg gcactggacg gtatcacggt cagtgaatat gagcgtgaca
     6301 gcctgcaccg tcaggtgata cgcacgcagg ggaaactggc gacgttcagc ggctacaacg
     6361 ccgacaaccg gttaagctgg cagcgcagcc tgccaggtgg cagccagaat ccacagcagg
     6421 cggtactccc ccggcgcaga acgacagcgt gacggcgcgg gactatatct ggaacgcaga
     6481 cggtgaagtc ggcggcatca atgacaaact gcgcggttgc ctggtgttca gctatgaccg
     6541 cagcggctgg ctgaccagcc gcacggggca gatgtatgac catgaccatt attattacga
     6601 caaagcgggc aacctgctga cggatgagta tcagggagcg gtgatggata accgcctgcc
     6661 gggttacggg cgtgaccgct accgctataa cgaatggggc gaactgacag agcgcaggga
     6721 ccagcagctg gagtggaacg cgcagggcca gttaacgcgg gtcatcagca gcaacagcga
     6781 gacgcggtat cagtacgatg cgctgggcag gcgcatcagc aaggcgacca gcaatctcca
     6841 taccgaccgg ggagaacgaa gccggacaac gtttgtgtgg gaagggttcc ggctgttaca
     6901 ggagacaacg tggcagggaa agcggacata cctgtacgat gcggaacagc cgtacacgcc
     6961 ggtggctgcc atcacggggc gcggagagag ccagaaaatc tggtattacc acacggacct
     7021 gacgggcacg gtacacgagg taaccgcgcc ggacgggacg ctggtgtggg cgggctacca
     7081 ggcgggattt ggtgaaaacc ggggcgacat cagcaacagc ggggcgtact ttgagcagcc
     7141 gcagcggctg ccggggcagt actttgacga agaaacaggg ctgcattaca atctgttcag
     7201 gtattatgcg ccggagtgcg ggaggttcgt aagccaggac ccgataggat taaacggtgg
     7261 gttgaactta tatgcttatg cgcctaatcc gttaggttgg atcgatccgc taggactgaa
     7321 ttctttagga atagattcaa ataatatatt tagaggagat ggtaactata aaggtggcgg
     7381 aattggacag ccaaagagtg gcacaatatc agcacaggac attattgatc atctctaaga
     7441 aaaaggtgat gggcgtttaa catcttttag tactaaaaaa acatctagtt ccaactctcg
     7501 tggtgcagat tttttctcaa aaaaacatgt cttcaaagtg gcagtttctg atctcgatga
     7561 tttagttaaa aataatcaga tcactgtttt aacacaagaa aaagtaaaag caatatttgg
     7621 tttagaatca agcaagaaaa ttagaaaaga acttggtaat attcttcaga atatggctag
     7681 gaataatgag gtgttagttc atgggcaaat tcttggaaat aaagttagcc gatgtaatta
     7741 gttagtggag aggaagttgt ggacgaaatt agaataagaa ttgctggtaa tacggctgcg
     7801 ccttgtttcc atgtgctaaa aaataaggga tataaagtaa caatggaaac tcatgcatta
     7861 tctgacgatt gtttagattg tgtatatgat tggtatgcgg aaaaaaatgg acaatttttt
     7921 agtgcaacga gtccagaaga gttacttgga ttagttgcaa tgtgggaagt tcgtggtaat
     7981 gactggagag ctaatttgaa tgagataaat acttatcaaa aagccataga agaatccccg
     8041 ttttttgata aaaacggcaa tgaaatagag tctgactaaa ttttattgat atagcaacga
     8101 taataaccgg aaatttcttc cagatctttc cggcttttta ttatttaact ttcgttcagc
     8161 acactaaaca tcccgtctcg catcccctta actggctgtt taacctgata aagttccctg
     8221 cgcagttcct gtacttcgtt gttatcccaa gcaggatcaa acatccctcc gagatcttca
     8281 cggtaacgcc cgtgccgatc tcaaatcccg cttttctcag ccatttggcg ctgattttga
     8341 tcaccggcgt gtcgctgcgg ttgggcagat aacccacggt acagcgacgc tgtgttttgg
     8401 aaatggtgac ttctggcgta atattgtgtt taagttaggg gctttcgtaa tctagcccag
     8461 cccccggccc taaacacaac ccccactcac cacaacctaa actcatccgc atcccgccat
     8521 gccggaaact tttctctata ttcccgcaat gccaccatcg acagctccgc atcaatacgc
     8581 gttgccttat gcgcgtcggc ggtagcgata atctcgcctt gcggattaat cacccggctg
     8641 tcaccgcgat aatggcagcc gttgccatcg ctgccgacgc gattgcatcc cgccacatac
     8701 gcctgattct caatcgcgcg ggccgtcagc aatgcctgcc agtgcagaga gcgcggagct
     8761 ggccagttgg cgacgtacag ggcgaggtca taatcgttga gattgcgcga ccacactgga
     8821 aaacgtaagt cgtagcacac cagcggcaaa atacgccagc cgcgccattc cacaatcact
     8881 cgcgcattgc ccgctttata atgcagatgc tcatctgcca tgcggaacag atgacgctta
     8941 tcataaaaat gtaccgtgcc gcccggctca accagcaaaa agcggttaac cgaaccagac
     9001 tccgtttgta atgcaacact gcctgcaatc agcgcattgc actgctgcgc cttagctgtc
     9061 atccagttca ctacgtcatc ttgtgcaagc gacgaagctg ccgcttccat ggcaaagccg
     9121 ctggtaaaca tctccggtag aacgatcaca tcgcgcccgg taataccttc cagttgacga
     9181 tcaaaatggc gcaggttggc aggaccatcc acccacacca acggttgctg caaaagcgta
     9241 atcttcaaac taggcacggt gtacaactcc tttatgcgaa gggttttata actttaacac
     9301 cttatcaggc agttgcctta gcgcagaata aattgataac aaatgctgat attggaaata
     9361 tctgatttgc aaattatcgt gttatcgcta ggctttagga ggttaataac atgggcagga
     9421 taagctcggg aggaatgatg tttaaggcaa taacgacagt cgccgcactg gtcatcgcca
     9481 ccagtgcaat ggcgcaggat gatttaacca ttagcagcct tgcaaagggc gaaaccacca
     9541 aagctgcgtt taatcagatg gtgcaagggc ataagctacc tgcatgggtg atgaaaggcg
     9601 gtacttatac tcccgcacaa accgtaacgt tgggagatga gacgtatcag gtgatgagcg
     9661 cgtgcaaacc gcatgactgt ggctcgcaac gtatcgctgt gatgtggtcc gagaaatcta
     9721 atcagatgac ggggctgttc tcggctattg atgagaaaac gtcgcaagag aaactcacct
     9781 ggctgaatgt gaacgatgcg ctttcgattg atggtaaaac ggtgctgttc gcggcgttga
     9841 ccggcagcct ggaaaaccat ccggatggct ttaattttaa ataattagcg gataaagaaa
     9901 cggagccttt cggctccgtt attcaattac gcggcttcaa ctttccgcac tttctccggc
     9961 aactttaccg gcttcgtcgc cagctcttcc ggatcaaagt catcaacgtt aatactgcgc
    10021 agacggcttt cttccgcttt caccagaata gcggcttcat ctttatcaat cagccccttc
    10081 gtcagcgcgt tgtgcgccag ttcatccaga cgggtaaatg gcagattttt acccagctct
    10141 ttacagatcc gctgatgaat cgggtcggcg gcaatcacat ccaccaacgc ctcttccagt
    10201 aaaccaaccg ggttatgctc gcttggcgtc aggtactgac cgcgaccaat acgtgaacga
    10261 gtggcgttcg gcacttgtaa aatcttcgcc attttatgat ccagcttgtc agacggtgcc
    10321 agataatgac gtccggtcgg gaagatcacc acattcagca gcccggcaac cacgcggttc
    10381 gggaagtttt gcagtaaatt gtccattgcc tgttctgcct gatacagcgc atcctgtacg
    10441 ccccagtgca ccagcggcag atcggcttca ttacggcctt cgtcgtcata acgcttcaga
    10501 acggcagagg cgaggtaaag ctggcttaaa atatccccca gacgtgccga gatacgctca
    10561 cgacgtttca ggctgccgcc cagcagtgcc atcgagacat cagaaagcag ggcgaggttg
    10621 gcgctcaggc ggttcaggtg ctgatagtag cgtttagtgg catcgccggt tggcgtgctg
    10681 ctggttaaac cgcgcgtcag gcccagccag aagctgcgaa ctttgttgct accgacgtga
    10741 ccgatatgtt tgaacaacag tttatcgaac gcgttgacgt cattgttctt cgccgcttcc
    10801 atctcttcca gcacgtacgg atggcaacga atcgctcctt gtccgaagat catcatgctg
    10861 cgggtcagaa tgttagcccc ttcaacggtg atggcaatcg gtgcgccctg gtaagcacgc
    10921 gccaggaagt tgctttgccc gagcataatg cctttaccgc cggtaatatc catcgcatca
    10981 ataatcgact gctgcccgcg gtgggtacag tgatacttaa cgatagccga cagcacggca
    11041 ggtttttcgc cgagcataat gccgtaggta atcagcgatg ccgcagcatc cattacataa
    11101 gcgttcccgg caatacgcgc cagcggctct tcaatccctt ccatcttacc gatagagatt
    11161 ttgaactgac ggcgaatgtg agaatacgcg cccgttgcca gcgctaccga tttcacgccg
    11221 ccggttgagt tggaaggcag ggtgatgccg cggcctaccg agaggcactc caccagcatc
    11281 cgccagcctt gcccggccat tttcggcccg ccgatgatgt aatcgatcgg cacgaagaca
    11341 tctttaccgc gcgtcggtcc gttctggaac ggtacgttca gcgggaagtg gcgacgacca
    11401 atttccacgc ccggcgtggt ggttgggatc agcgcacagg taatgcctaa atcttctgca
    11461 ccgccgagta atttttccgg gtcggagagt ttaaacgcca gcccaagcac ggtcgcaatc
    11521 ggtgccagcg taatgtagcg tttgttccag gtcagacgca tccccagcac ctgctggccc
    11581 tgccattcgc ccatgcagac aatcccggtg tccggaatcg cgcccgcatc ggaacccgct
    11641 tccgggctgg tcagtgcaaa gcaggggatc tcctgaccac gcgccagacg cggcagatag
    11701 tgatctttct gctcgtcagt gccgtaatgt tgcaacagtt cgcccgggcc taatgagttt
    11761 ggcacgccga cggtaatcgc caggatcccg ctcacgccag agagtttttg cagcacacga
    11821 gactgggcat aagctgagaa ctccagtccg ccgtactctt ttttgataat catcgcgaag
    11881 aagcgatgct ctttaaggta cgcccacaac tccggtggca gatccgccag ctcatgagta
    11941 atctggaaat cattcgccat ccggcaggct tcttctaccg ggccgtcgag aaacgcttgc
    12001 tcttcagcgg tcaggcgcgg ctgcggatag ttatgcagct ttttccagtc cggcttgccc
    12061 tggaacaagt cgccctccca ccaggtggtg cccgcatcaa tcgcttcttt ctcagtgcgc
    12121 gacatcggcg gcatcacctt acggaaaccg cgaaataccg gcgcggaaat catcgactta
    12181 cgcataggcg caaagttaaa tggcacgagg ataatggtca gaggcaccag tacccacgcc
    12241 gaccacagac cagcaacgcc gagggcggct gtccaggcga gcaaaatcag actgctgata
    12301 aataagctca cgcggtgata gaacaacgcg ccgagcagga caaccgtagc gagaatactc
    12361 aaaatcatca taacgaaaag ccccttactt gtaggaggtc tgaccacttg tgatgatatg
    12421 gttgtagtgg atgtaaaaac atttagcaat gtgtttacaa tataattaca acaaagctca
    12481 cattgttgct gtttttatcc gcacttcagg tcaaaaagtc ctggtcatag cacctgcccg
    12541 tacttctcgc ttttggcggt atccggtaca ctgcattttg tctattacat ttatgctgaa
    12601 ggatatcctc atgtaccagg atcttattcg taacgaactg aacgaagcgg cggaaacgct
    12661 ggctaacttt ttaaaagatg acgccaatat tcacgccatt cagcgcgcgg cggtcctgtt
    12721 agcagacagc tttaaagccg gtggcaaagt gctttcctgc ggcaacggcg gttcccattg
    12781 cgacgctatg cactttgccg aagagttgac cggtcgctac cgtgaaaacc gtccgggcta
    12841 cccggcgatt gctatttctg acgttagtca tatttcctgc gtcggtaatg atttcggttt
    12901 caatgatatt ttctcccgct acgttgaagc ggtaggtcgc gaaggcgatg tactgctggg
    12961 gatctccacc tccggtaact ctgcaaacgt gatcaaagcg atcgcagcgg cgcgtgagaa
    13021 ggggatgaaa gtgatcaccc tgaccggtaa agacggcggt aaaatggctg gcacggcgga
    13081 tatcgaaatt cgcgttccgc actttggtta tgccgaccgc attcaggaga ttcacattaa
    13141 agtgatccat atcctgatcc agttaattga aaaagagatg gttaagtaag tctggcgtag
    13201 gccggataag gcgtttacgc cgcatccggc acttgtgcgc tgatgcctga tgcgatgctg
    13261 acgcgtctta tcatgcctac aaatctgtac ccgaaccgta ggccgaataa ttcgtttacg
    13321 ccgcatccga cctgaaaatt cttaaatcaa tcttcgccgg ggtccatgcg ctcccgctgt
    13381 tgtggaggtt acccatgtgc gaattgctcg ggatgagcgc caacgtccct accgatatct
    13441 gctttagttt caccggactt gtacagcgtg gtggtggaac cgggccacat aaagatggct
    13501 ggggcattac cttttacgaa ggtaaaggct gtcgcacatt taaagatcca caacccagct
    13561 ttaattcccc catcgccaaa cttgtccagg actacccgat aaaatcctgt tcggtggtgg
    13621 ctcatattcg ccaggctaat cggggcgagg tggcgctgga aaatactcac ccatttaccc
    13681 gcgagttatg ggggcgtaac tggacttatg cccataacgg acaactgacg ggctacaaat
    13741 cactggaaac cggcaacttc cgcccggtcg gtgaaaccga cagcgaaaaa gccttttgct
    13801 ggctgctgca taaattaacg cagcgttacc cgcgcacgcc gggcaacatg gcggcagtgt
    13861 ttaaatatat cgcctcactg gcggatgaac tgcgggcgaa gggcgttttc aacatgctgc
    13921 tttcggacgg gcgctatgta atggcgtatt gctcgactaa tttacactgg atcacccgcc
    13981 gcgcgccgtt tggcgtggca acgttgctgg atcaggatgt ggaaatcgac ttcagctcgc
    14041 agaccacacc gaatgatgtg gtcacggtga ttgcgacaca gccgctgacg ggcaatgaaa
    14101 cctggcaaaa aattatgcca ggcgaatggc gcttattttg cctcggggag cgtgtagttt
    14161 gatgccagtt gtggctgcac cacttcgtgg cttaacggct tgctgaccac gtaacggcca
    14221 ttggccacag aaacggtcgg tggcttacgg gtttgctcaa agtagtcgta gcccggcttc
    14281 agctgctccc agaagtcctt aaagttggaa tatttatggc gcttcatatt ggcgtcggtc
    14341 atgcggaatg ggtaaatact cacctgcacg ctcggctgac caaacaccag cgcaccagta
    14401 acgaactgga agatctcatc aataccctga ttggtcattg cgtagcagcc gatggataca
    14461 caatcgccgt ggatcatcag gtatttccct tcataaccat gcgcacggtc ataagcattg
    14521 gggaatccaa tattaatcgc tttgtagtaa cggctgtctg gttttaactg attacgctgg
    14581 acgctataaa acccttccgg gcttttgaaa tcgccctgac gctgttttgg ccctaagccg
    14641 ccggaatatt tacagatttt ataactgtcg agcagttgat attgctcgcc cattttgacg
    14701 taaagatcga gcgtacgttc ttccttgaag atctggatgt agacagggga tcccatcaac
    14761 tgctgcttat actctttgct cactggcgtg gtcgagctac tgctgcccag caaaccggca
    14821 aacgaaacgc acgggatcaa caacatcgca agaattaatg cgattttacg catactgctt
    14881 attccttgat aaaacggtta cacacgccag gacggcaaaa tggatcccaa atcggaatag
    14941 tctggatttg gaaggctcac attatcacca aaagagtttt acgcaagcct gtcggcgctg
    15001 ggtttacaaa tttagcacag gggcagtttt caatagtccg aaaagcgcat gtatcattta
    15061 tttcctaata ttatgaaatc tcaatagtaa tatgcaaata agcaattaat aatcccgttt
    15121 actcctccgg tcttttaaaa ttctcacctt cattcagccg gtttatgtct ttcatgtctt
    15181 cgttcttgct cggcagattt ttgcatccag gcgtgttttc gttatgcgtt ttgctccctt
    15241 tactcgccag cgccaccaca accgggagca gataaaacag catccagaat ggtttcctgc
    15301 gccgctcaag gcgagtgaca gacgctggca ggcgctggcg gaaaacaacc actttttaag
    15361 cagcgatcac ctgcataaca tcaccgaagt ggcgattcac cgcctggagc agcagcttgg
    15421 caagccttac gtctggggcg gcacgcggcc cgatcaaggc ttcgattgta gtgggttagt
    15481 tttttatgcc tacaacaaga tccttgaggc gaaactcccg cgcacggcca atgagatgta
    15541 ccactaccgt cgggcaacga ttgtggcgaa taacgacctg cgccggggag atttgctatt
    15601 tttccatatc cacagccgcg agattgccga ccatatgggg gtgtatctgg gcgacggtca
    15661 gtttattgag tcactgcgca ccggggaaac cattcgggta agccggttag ccgaaccttt
    15721 ctggcaggac cattttttag gcgcgcgcag gattttgacg gaagagatga ttttgtaggc
    15781 cggataaggc gtttacgccg catccggcgg ttgtgcacgg atgcctgatg cgacgctgac
    15841 gcgtcttatc atgcctacaa atctgtgcgt ttttgcgagc cgctttcccg atataacaca
    15901 gcgtcattgt cggcctttcc ttttcctctt accgttgaga aaaaggagtc gctatgtcgg
    15961 aatatcgtcg ttattacatc aaggggggaa catggttttt cacggtaaat ttacgcaatc
    16021 gtcgaagcca tcttctgacc acccagttcc agacgctccg taacgccatt attaacgtta
    16081 agcgagacag accttttgaa attaacgcct gggtcgtttt gccggagcat atgcattgca
    16141 tatggacatt acccgaaagc gatgatgatt tttcctctcg ctggcgggag attaaaaagc
    16201 agttcaccca tgcttgcgga ttgaaaaata tctggcaacc gcgtttttgg gagcatgcca
    16261 ttcgcaatac aaaagattat cgccatcatg ttgattatat ttatataaat ccagagaaac
    16321 atggctgggt aaagcaggtg agtgattggc cattctcaac gttccatcgc gatgtcgcgc
    16381 gagggttata tcccatcgat tgggcgggag acataacgga tttaagtgcc ggggagcgta
    16441 tcattttata actgtgcgct ggtgccggat gcgcttacgc ttatccggcc tacaaatcgt
    16501 gctttaacgg taggccggat aaggcgttca cgccgcatcc ggcagttatg cgcagatgcc
    16561 tgatgcgacg ctggcgcgtc ttatcatgcc taaaaacttg tgcctgaacg gtaggccgga
    16621 taaggcgttt acgccgcatc cggcaattgt gcaccgatgc cggattctta catcaacgcc
    16681 cccataatct tcaactccag ctcatccggc acttcgttat acgacagcac atgcagcccc
    16741 ggcgcaaaca accttgcata acgcggcagc aaagggcgta gctgcggcgg caccagcagc
    16801 accggatctt tccccgccgc tttcatctgc tctttcacct gtggcatcgt gctctggaac
    16861 tggttgagca tattcgggtc aaccggcacg ctgtcgagca tcactttgcc ggcctgttgc
    16921 gcctgattca ctacgttggt cagcagattt tccagctcat tattcagcgt atacaccgtc
    16981 agctcctgct tgcgaacgaa cggatgagta atgctgcgcc gcagcgccag gcgcacatcg
    17041 gccgccagca gaatatgatc tttagtcacc gtgctactgg cgaccagcac ggtggcgatg
    17101 gtgacgatat cgcgcaggga aacgccttcg gtcagcagcg cccggtacac tttcagcaat
    17161 tggctgtaat tgagcgccgc gcttaaatcc tccgccaggc gcggtgccat cgacgacaaa
    17221 cggttatgca actgcgtgat gtcatcatag ttaaacaaat caggaatata gctgcgcaca
    17281 atcttgttca catgggtggc aatcacactg gcgctgtcga tcacctgata ccccatattc
    17341 agagccttcg ctttctgcgc aggctggatc caggtgaccg gtatcccgta cgccgggtcg
    17401 ttccccagca cgccgtcaat ctcgccgtag gtttcgctgg agggcagcgc catcagttta
    17461 tccgccggaa tatccgcttc atcagcctca atgccgttga tgaaaatagc gtactggctg
    17521 ggcttgaggc ggaagttttc ccgaatgcgg atctccggca gcagcacgcc gttaccgtcg
    17581 gaaatcacct gccgaacgcc gcgaatccgc tgggtgagcg ggttgccctg cgctttgtcc
    17641 accagcgcca ccagcttgta gccgaggctt aagctgatgg gctcgatcag cggaatagtt
    17701 tcccaactga cctgttgttc gctggtttca gtgatggtgc gggtcagcgt ttcgaggctt
    17761 ttctcttccg cctccgccgc ctgcgggcgt ttgctcatcc gccagccggt aaagccaagc
    17821 agggcgctga acagcaaaaa cggcagatgc ggcatccccg gcaccaccgc cagcacgaac
    17881 atgatcccgg tggcggtgta gagcaccgac gggcttgcca gcaactggtg gcgcacgtca
    17941 tgggcgatat cgccgttgtc gctgacgcgg gtgacgataa tcgccgccgc ggtggagagc
    18001 agcagggaag ggatctgcgc caccaggccg tcgccgatgg tcatcagcac gtattgctgg
    18061 aaggcggcgt cggcgctcag gttgtatttg aagatgccga tacagacgcc gccgctcagg
    18121 ttgatcgcca gaatcatcat cccggcgatg gcgtctccgc gtacaaactt cgatgccccg
    18181 tccatcgcgc cgtagaaatc ggcctcgctg gcaacatctt tacgccgggt ttgcgcctgc
    18241 gcctggttga tcaatccggc gttaagatcg gcgtcaatcg ccatctgttt gccgggcatc
    18301 gcgtccaggg taaagcgggc agaaacctcg gaaatacgct cggcctcttt ggtgacgacg
    18361 ataaagttga ttgtcaacag cgtcagtaaa tccgagcgtg aaagcattat cgccgcgctg
    18421 cacgggcagt cgatttttaa cggtggtggg ctgtcgccgt taaataaaat cagcccgtct
    18481 catccgccaa aacctgccac cgtggcggtg ccggaagaga cggagaaaaa agcgcgtgat
    18541 gtgaatgaga aaacggcgct gctgaagaag aaatccgcca ccgagcttgg cgagttggca
    18601 accagtatca acaccattgc ccgtgatgcg catatggaag cgaatctgga gatggagatt
    18661 gttccccagg gattacgcgt gctgattaaa gacgaccaga atcgcaatat gttcgaacgc
    18721 ggcagcgcgc agattatgcc gttctttaaa actttgctgg tggagctcgc gccagtgttc
    18781 gactcgctct ataacaaaat tattattacc gggcataccg atgcgatggc ctacaaaaac
    18841 aatatctaca acaactggaa cctttcgggc gatcgcgcgc tttcagctcg tcgggtgctg
    18901 gaagaggccg gaatgccgga agataaagtg atgcaggtaa gcgcaatggc ggaccagatg
    18961 ctgctggacg cgaagaatcc gcaaagcgcg ggcaatcggc gtattgagat tatggtgctg
    19021 accaaaagtg cgtccgatac gctgtatcaa tactttggtc agcatgggga taaagtggtg
    19081 cagccgctgg tgcaaaagct ggataagcag caggtgcttt cgcagcgaat gcgctaaatg
    19141 ctgaatcttt acgcatttct caaaacctga aatcactgta tactttacca gtgttgagag
    19201 gtgagcaatg cgtaaaatca ttcatgtgga tatggactgc tttttcgcag cggtggagat
    19261 gcgcgacaat cccgctctgc gcgatatccc tattgctatt ggcggcagcc gcgaatgtcg
    19321 gggggtgatc agcaccgcca attatcccgc gcgtaaattt ggcgtacgta gcgctatgcc
    19381 tactgggatg gcgctcaaat tatgcccaca tctcaccttg cttccggggc gctttgacgc
    19441 ctacaaagaa gcctcaaatc atatccgtga aatcttctcg cgctacacct cgcgcattga
    19501 accgttgtca ctggatgagg cttatctcga tgtcaccgat agcgtccatt gccacggttc
    19561 tgcgaccctc atcgcccagg aaatccgcca gacgatcttc aacgagctgc aactgacggc
    19621 atctgcgggc gtggcaccag taaagtttct cgccaaaatc gcctccgaca tgaataaacc
    19681 caacggtcag tttgtgatta cgccggcaga agttccggca tttttacaaa ccttaccact
    19741 ggaaaaaatc cccggcgtcg gcaaagtctc ggcggcaaaa ctggaagcga tggggctgcg
    19801 gacctgcggt gatgtacaaa agtgtgatct ggtgattctg cttaaacgct ttggcaaatt
    19861 tggccgcatt ttgtgggagc gtagtcaggg gattgacgag cgcgacgtta acagcgaacg
    19921 gttacgaaaa tccgtcggcg tggaacgcac gatggcggaa gatattcatc actggtctga
    19981 atgtgaagcg attatcgagc ggctgtatcc ggaacttgaa cgccgtctgg caaaggtaaa
    20041 acctgattta ctgattgctc gccagggggt gaaattaaag ttcgacgatt ttcagcagac
    20101 cacccaggag cacgtctggc cgcggctgaa taaagctgac ttaatcgcca ccgcgcgtaa
    20161 aacctgggat gaacgccgcg gcgggcgcgg tgtgcgtctg gtggggctgc atgtgacgtt
    20221 gcttgacccg caaatggaaa gacaactggt gctgggatta tgatgtatac tattatgtat
    20281 attctggtgt gcattattat gagggtatca ctgtatgcat cgaattctcg ctgaaaaatc
    20341 ggtcaatatc actgagttac gtaaaaaccc agctaaatac tttattgatc aaccagttgc
    20401 ggttctttct aataatcgcc ccgcaggata tctcttaagt gccagcgcat tcgaagcgtt
    20461 aatggacatg cttgctgaac aagaggagaa aaagcccata aaggcgcgct ttcgtccaag
    20521 tgctgcaaga ttagaggaaa ttacacgccg cgctaaacaa tatcttaatg atatgacgga
    20581 tgatgatttc aatgacttta aggaataagg atgcgggtat tcaaaacaaa acttattcgc
    20641 ctgcaactta cagcagagga acttgatgcg ttaacggcgg attttatttc ctataagcgt
    20701 gacggtgttt tgccagatat atttggtcgc gatgcactct acgacgactc gtttacctgg
    20761 ccattaatca aatttgagcg agttgctcat attcatctgg caaatgtgaa taatccattt
    20821 ccgccacagt tgcgccaatt cagcagaacg aatgacgaag cgcatttggt atattgtcag
    20881 ggtgcgtttg atgagcaagc atggttgctc attgccattc tgaagcctga acctcataaa
    20941 ctggctcgag ataacaacca aatgcataaa attggaaaaa tggcagaagc gtttcgcatg
    21001 cgtttttgaa tttatattat gaataacata caaataagag attatcagcc tggcgatttt
    21061 cagcaactat gcgctatttt cattagagcg gttacgatga ccgtcagtca gcattattca
    21121 ctacaacaaa ttgccgcctg ggcgcagatt gacgaatctc gctggaagga gaaactcgcg
    21181 aaatcacaag tgcgggttgc agttattaat gcaaaactgg tcggctttat tacctgcgtt
    21241 gaacactata tcgatatgtt atttgttgac cctgaataca cccgccgtgg ggttgccagc
    21301 gctttgttaa aaccttttat taagtctgaa tccgaactta cggtggacgc aagcataacc
    21361 gcaaaaccct tttttgaacg ttatggtttt cagacagtta agcagcagcg cgttgaatgc
    21421 cggggagcgt ggtttattaa tttttatatg cgttataaac cgcaacatta aatccagctt
    21481 gcaatgaaaa taacgcccgc ctagtatgtg cgcgttttct tattccacaa actgcaagga
    21541 ggtaaatcat gacaaatcct ttatcaatga ctctttgcag acctttccag gattaattct
    21601 ttttttcttg ccctggattc gtctgccatt tcctgatttt ttaattgtta ctctcaataa
    21661 ttcaagtcgc agcacatgca acttgaagta tgacgagtct atttacggaa tggtatatgg
    21721 gcaattatat tcgtccctta tccgatgcgg tatttaccat cgcatctgat gacctgtgga
    21781 tcgagagttt agcgatccaa caattacaca ccacggcaaa tttacccaac atgcagcgcg
    21841 tagtggggat gccagattta caccccggac gcggctaccc gattggcgca gcgttcttct
    21901 ccgcaggccg tttttaccca gcactggtcg gcaatgatat cggctgtggc atggcgctat
    21961 ggcaaacaga tattctcgct cgcaaataca acgccgataa gtttgaaaag cgattatcag
    22021 ctttggatga cgttgctgaa gaaagctggc tggaggaaaa cctgccgtca gcatttgcac
    22081 agcatccgtg gcgcagctcg cttggctcta tcggtggtgg caatcatttc gctgaactgc
    22141 aacaagttga tcaaatcatc aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc
    22201 atctgcaact gctggttcat agcggctcga gggggctagg gcagtctatt ttacagcggc
    22261 atattgcctc gttttcgcat catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg
    22321 cgcgttttaa tcgccacctg atcgctttgc gcataatgca acaggttaag gccaccggaa
    22381 gtccggttct ggatgtggcg cataactttg ttagcgcgtg tcgaatcggt gatcaacaag
    22441 gggtcttaca tcgtaaaggt gccacgcctg atgactgtgg tctggtggtt attcccggtt
    22501 cacgcggtga ttactccagg ctggttcagc ccgtcaggag tgaggaaaca ttgcattcgc
    22561 tggcgcatgg ggctgggcgt aaatgggggc gcaccgagtg taaagggcgt ctggcagcga
    22621 aatacacagc gacgcaactc tcgcgtactg aacttggcag tcgggtaatt tgtcgcgata
    22681 aacaactcat cttcgaagaa gcgccacaag cctataaatc ggctgaaagt gtggtgcaat
    22741 gtcagggcag gccggattaa ttattcctgt cgcgcggctg cgtccggtgc taacgctcaa
    22801 aaacagtgga gggaaaaaag gatgatcttg ctataactct cctcagcgca ggggccggaa
    22861 gaatgttgtc tcgcagtgaa aaaggcgctg gacaggctaa ttaaagaagc tgcccgacaa
    22921 gacgtcgcgg taacggtgct ggaaacagaa acaggccgct actctgacac gctgcgttcg
    22981 gcgctgattt ctctggatgg cgacaacgca tgggcgttaa gcgaaagctg gtgtggcact
    23041 attcagtgga tttgcccgag cccgtatcgc ccacatcacg ggcgtaaaaa ctggtttctg
    23101 ggcattgggc gttttaccgc tgatgagcag gagcaatccg atgcgatccg gtatgagacg
    23161 ctgcgttcgt cggggccggg cggtcaacat gtcaataaaa ccgactcggc ggtacgcgcc
    23221 acgcatttgg catccggtat tagcgtgaag gttcagtcag agcgcagcca gcatgctaac
    23281 aaacggctgg cgcgattgct gattgcctgg aagctggatc agcagcaata ggaaaatagc
    23341 gcggtgctga aatcacagcg gcgaatgttc catcaccaga ttgaacgtgg caacccgcga
    23401 cgaacgttta cagggatggc ttttatcgaa ggataatgaa ggatgaaact gccggaaggc
    23461 gattaaacgc catccggcag caaatcaaat aattacttcg ccggaatttc tttcagcagt
    23521 tcagtcagca gtgtccagta atgacctacg ctttcgatgt gaacttgctc atccggagag
    23581 tgtggaccgg tgatagttgg cccgatagaa accatgtcca tttccggata cggttttttg
    23641 aacagaccac attccaggcc cgcgtggata atctggatgt tcggcgtctt gttgaacagg
    23701 cgctgatagg tttcacgtac cagatgcatc accggagaat tagcgtccgg ctgccagcca
    23761 ggatatgcgc ctttcgcttc ggttttcgcg ccagccagtt tacccagcga atccagcatg
    23821 ctcaccacgt agtctttacc gctgtcgatt agtgaacgga tcaggcagtg aatttctacg
    23881 ttattgtcag tcatggtcac cacaccgacg ttcagggagg tttcgaccac accttttgcc
    23941 acgtcggaat tgcggatcac accgttcggg gtggcgttca gcagacgaat aaaggtatcg
    24001 cgagatttcg caatcagggc agctttatcg ttcgctacag agtccagcaa caaggccaga
    24061 tttttctctt tctctgccag ctcgtttttc aggatctcct gataggtatt caccagagat
    24121 ttcagtgcgt cgactttatc agctgcgaca gcaatggtcg caaaggcttc acgcgggatg
    24181 gcgttacgca gtgtgccgcc gttgaaatcg ataaggcgca ggtccagttc ttctgcatga
    24241 cccgccagga agcgcaccag cagtttgttg gcattaccca ggccaacgtg gatttcaccg
    24301 ccggagtgac cgcctttcag accttttaag gttaacttga aggtttcaaa accagctgga
    24361 accgcttcac gatctaaatg caggttggag gtgaagtcga taccccccgc acaacccatg
    24421 tagatttcac cttcttcttc ggagtcggtg ttaatcagaa tatcagcctg caaccagttg
    24481 ctctgtaagc cgaacgcacc gtccataccg gcttcttcgg tcatggtcag cagcacttcc
    24541 agcgggccgt gaaccacgtt ttcgtcagcc agaaccgcca gtgcagaggc cataccaatg
    24601 ccgttatccg cacccagcgt ggtgccgcgc gctttaaccc attcgccatc aatataaggc
    24661 tggataggat ctttcgtgaa gtcatgcacg gtgtcgttat ttttctgcgg caccatatcg
    24721 aggtgggcct gtaagacgac cggtttacga ttttccatac ctgcggtagc aggttgacga
    24781 atcaggatat tacctacctg atcgcgttcg acatggaaac ctttctcttt tgcccaacca
    24841 acaatgtatt cagcgagttg ctcttcatga taggacgggt gaggaataga acagattttg
    24901 gcaaaaatat cccacagcgg ctgtggagat aattgagaca gttcagacac gttaagtctc
    24961 cttgtcgatc acccgcaaaa cagtattgca ggtcacaggg ttagcagaaa atgttgtcaa
    25021 cacaagacag gcttgcgaga tatgtattga gaataccact ttatcccgcg tcagggagag
    25081 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
    25141 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
    25201 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
    25261 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
    25321 gtggcggtct ggtaccgggg gcgttactgg cgcgtgaact gggtattcgt catgtcgata
    25381 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
    25441 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
    25501 ctgttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
    25561 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggatcgaac
    25621 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
    25681 gcctggcatt gccgggcgtt gttcttttta acttcaggca ggttacaata gtttccagta
    25741 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
    25801 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
    25861 cctgtgcagt cggctcttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
    25921 ctgatgtgga tctggcgcgg tattgaccca cgtgaaatcc tcgacgtcca ggcacgtatt
    25981 gtgatgagcg atgctgaacg taccgacgat gatttatacg ataccgtgat tggctaccgt
    26041 ggtggcaact ggatttatga gtgggccacc caggcgatgg tgtggcaaca aaaagcctgt
    26101 acggaagaag atccgcaact cagtggtcga cactggctgc atgcggctac gttgtacaac
    26161 attgccgcct atcctcatct gaaaggagat gacctggccg agcaagcgca ggctttatca
    26221 aaccgcgcct atgaagaggc cgcccagcgt ctgccgggca cgatgcggca gatggagttt
    26281 accgtacccg gcggtgcgcc catcaccggc tttttgcata tgccgaaagg cgatggcccg
    26341 ttcccgacag tattaatgtg tggtggtctg gatgcgatgc agacggacta ttacagcctg
    26401 tatgaacgtt attttgcgcc gcgcggcatt gcgatgctga ctattgatat gccgtcggtg
    26461 ggcttttctt caaaatggaa gctcacccag gactccagcc tgttgcatca gcacgtctta
    26521 aaggcgctgc ctaacgtacc gtgggtggat cacactcgcg tcgcggcctt tggtttccgt
    26581 ttcggcgcta acgttgccgt gcgtctggca taccttgaat cgccgcgtct gaaagcggtt
    26641 gcctgtcttg gtccggtagt tcataccctg ttgagtgatt ttaagtgcca gcaacaggtg
    26701 ccggaaatgt atcttgacgt tctggcgagt cgtttgggga tgcatgatgc ttccgacgaa
    26761 gcgttacgcg tggagctgaa tcgctattca ttaaaagtgc aaggattgct ggggcgtcgc
    26821 tgcccaacgc caatgttatc aggctactgg aagaacgatc cgttcagccc ggaagaggac
    26881 tcacgcttaa tcacctcatc atctgctgac ggtaaattat tagagatccc atttaacccg
    26941 gtgtatcgga attttgacaa aggtcttcag gaaatcaccg gctggatcga aaaacgcttg
    27001 tgttaaaaat ttgctaaatt ttgccaattt ggtaaaacag ttgcatcaca acaggagata
    27061 gcaatgacgt taccgagtgg acacccgaag agcagattga tcaaaaaatt taccgcacta
    27121 ggcccgtata ttcgtgaagg taagtgcgaa gataatcgat tctttttcga ttgtctggct
    27181 gtatgcgtca acgtgaaacc ggcaccggaa gtgcgtgaat tctggggctg gtggatggag
    27241 ctggaagcgc aggaatcccg ttttacctac agctaccagt ttggtctgtt cgataaagca
    27301 ggcgactgga agagtgttcc ggtaaaagat actgaagtgg ttgaacgact ggagcacacc
    27361 ctgcgtgagt ttcacgagaa gctgcgtgaa ctgctgacga cgctgaatct gaagctggaa
    27421 ccggcgaatg attttcgtga tgaaccggtg aagttaacgg cgtgagtgaa atgtgccgga
    27481 tgcatcacat ccggcaatat tcattaaaac tgatacgtca tcccaaccgc gacagtatca
    27541 tcattattaa tattcagctt attatcgcta tccagttggt tgattttata atcaacaaac
    27601 gctgacatat ttttgttgaa ataatacgta gcaccgacgt cgatataatt gaccagatct
    27661 tcatcaccga tgccctcaat atctttccct ttcgataaga cataacccag cgatggacgc
    27721 agaccaaagt caaactggta ttgagcgacc gcttcaaagt tctgtgtctt attggcaaag
    27781 ccgccagtta ttggcgtcat tttgcgtgtt tcagaataga aagttgccag ataaatatta
    27841 ttggcatcgt atttcagacc tgttgcccat gcttctgcac gcttgcctgt gccacggctt
    27901 tgcaggttct gctcgttggt gcgatctgag ttggtatagg ccccactaat ggcgaaatcg
    27961 ctgccgccaa agtcatatgt caatgacgtg ccgaagccat cgccgttttg ctttttaacg
    28021 tcgcggtttt cgtttttccc ttgatattgc agggttaagt tcaggccatc gataacgccg
    28081 aagaagtcgg tgttccgata cgtcgccaga ccgctggcgc gtttggtcat aaagttgtcg
    28141 gtctgcgccg aggaatcgcc accaaattcc gggaacatat cggtccaggc ttccacgtca
    28201 tacaacgcgc ccaggttacg accataatcg aaagaaccca aatctttata tttcaacccg
    28261 gcaaaagcga gacgcgtttt ttgctgtgca gtatcactct ccgctttatt tccggcaaac
    28321 tccgcttccc accgaccata accagtcagt tgatcgttaa tttgagtttc acctttaaaa
    28381 ccaaaacgga tataactctg gtcgccatct ttactggcgt tatcactcat ataatgcatg
    28441 gctttaactt tgccatagac atccagttta ttaccgtctt tattatatat ttctgcagcc
    28501 tgtacagatg cagatgccac aatgcccatc accactaatg ccagagtgct ctttttcatt
    28561 ttcattcctg attttaatta acgcgcgaat attcagcggg agagccccgt tgaaaacagg
    28621 aaagttttta acctgagatt gttaaagata tattacagat taatgatatt
//
LOCUS       DAZOYF010000036        28183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZOYF010000036 DAZOYF010000000
VERSION     DAZOYF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.036"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="ACHUOF_001497"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="ACHUOF_001497"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR0610733.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="ACHUOF_001498"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="ACHUOF_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR0610734.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHUOF_001499"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHUOF_001499"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR0610735.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="ACHUOF_001500"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="ACHUOF_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR0610736.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="ACHUOF_001501"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="ACHUOF_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR0610737.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHUOF_001502"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHUOF_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR0610738.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHUOF_001503"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHUOF_001503"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR0610739.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9168
                     /gene="atpI"
                     /locus_tag="ACHUOF_001504"
                     /pseudo
     CDS             8747..9168
                     /gene="atpI"
                     /locus_tag="ACHUOF_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
     gene            9177..9992
                     /gene="atpB"
                     /locus_tag="ACHUOF_001505"
     CDS             9177..9992
                     /gene="atpB"
                     /locus_tag="ACHUOF_001505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR0610740.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            10039..10278
                     /gene="atpE"
                     /locus_tag="ACHUOF_001506"
     CDS             10039..10278
                     /gene="atpE"
                     /locus_tag="ACHUOF_001506"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR0610741.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10340..10810
                     /gene="atpF"
                     /locus_tag="ACHUOF_001507"
     CDS             10340..10810
                     /gene="atpF"
                     /locus_tag="ACHUOF_001507"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR0610742.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10825..11358
                     /gene="atpH"
                     /locus_tag="ACHUOF_001508"
     CDS             10825..11358
                     /gene="atpH"
                     /locus_tag="ACHUOF_001508"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR0610743.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11371..12912
                     /gene="atpA"
                     /locus_tag="ACHUOF_001509"
     CDS             11371..12912
                     /gene="atpA"
                     /locus_tag="ACHUOF_001509"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR0610744.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12963..13826
                     /gene="atpG"
                     /locus_tag="ACHUOF_001510"
     CDS             12963..13826
                     /gene="atpG"
                     /locus_tag="ACHUOF_001510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR0610745.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13853..15235
                     /gene="atpD"
                     /locus_tag="ACHUOF_001511"
     CDS             13853..15235
                     /gene="atpD"
                     /locus_tag="ACHUOF_001511"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR0610746.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15256..15675
                     /locus_tag="ACHUOF_001512"
     CDS             15256..15675
                     /locus_tag="ACHUOF_001512"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR0610747.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            16026..17396
                     /gene="glmU"
                     /locus_tag="ACHUOF_001513"
     CDS             16026..17396
                     /gene="glmU"
                     /locus_tag="ACHUOF_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR0610748.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17558..19387
                     /gene="glmS"
                     /locus_tag="ACHUOF_001514"
     CDS             17558..19387
                     /gene="glmS"
                     /locus_tag="ACHUOF_001514"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR0610749.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19549..20370
                     /locus_tag="ACHUOF_001515"
     CDS             19549..20370
                     /locus_tag="ACHUOF_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR0610750.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20357..22465
                     /locus_tag="ACHUOF_001516"
     CDS             20357..22465
                     /locus_tag="ACHUOF_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0610751.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22462..24129
                     /locus_tag="ACHUOF_001517"
     CDS             22462..24129
                     /locus_tag="ACHUOF_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0610752.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24132..25658
                     /locus_tag="ACHUOF_001518"
     CDS             24132..25658
                     /locus_tag="ACHUOF_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR0610753.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25659..27275
                     /locus_tag="ACHUOF_001519"
     CDS             25659..27275
                     /locus_tag="ACHUOF_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR0610754.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27331..27483)
                     /locus_tag="ACHUOF_001520"
                     /pseudo
     CDS             complement(27331..27483)
                     /locus_tag="ACHUOF_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(27506..27883)
                     /locus_tag="ACHUOF_001521"
     CDS             complement(27506..27883)
                     /locus_tag="ACHUOF_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610755.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
BASE COUNT         7297 a         6549 c         7273 g         7064 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcagtctt tctgcctaac
     8941 gttttgttta tgatatttgc ctggcgtcac caggcgcata caccagcgaa aggccgggtg
     9001 gcttggacat tcgcatttgg cgaagctttc aaagttctgg cgatgttggt gttactggtg
     9061 gtggcgttgg cggttttaaa ggcggtattc ttgccgctga tcgttacgtg ggttttggtg
     9121 ctggtggttc agatactggc accggctgta attaacaaca aagggtaaaa ggcatcatgg
     9181 cttcagaaaa tatgacgccg caggattaca taggacacca cctgaataac cttcagctgg
     9241 acctgcgtac attctcgctg gtggatccac aaaacccccc agccaccttc tggacaatca
     9301 atattgactc catgttcttc tcggtggtgc tgggtctgtt gttcctggtt ttattccgta
     9361 gcgtagccaa aaaggcgacc agcggtgtgc caggtaagtt tcagaccgcg attgagctgg
     9421 tgatcggctt tgttaatggt agcgtgaaag acatgtacca tggcaaaagc aagctgattg
     9481 ctccgctggc cctgacgatc ttcgtctggg tattcctgat gaatctgatg gatttactgc
     9541 ctatcgactt gctgccgtac attgctgaac atgtactggg tctgcctgca ctgcgtgtgg
     9601 ttccgtctgc ggacgtgaac gtaacgctgt ctatggcact gggcgtattt atcctgattc
     9661 tgttctacaa catcaaaatg aaaggcatcg gcggcttcac gaaagagttg acgctgcagc
     9721 cgttcaatca ctgggcgttc attcctgtca acttaatcct tgaaggggta agcctgctgt
     9781 ccaaaccagt ttcactcggt ttgcgactgt tcggtaacat gtatgccggt gagctgattt
     9841 tcattctgat tgctggtctg ttgccgtggt ggtcacagtg gatcctgaat gtgccgtggg
     9901 ccattttcca catcctgatc attacgctgc aagccttcat cttcatggtt ctgacgatcg
     9961 tctatctgtc gatggcgtct gaagaacatt aatttaccaa cactactacg ttttaactga
    10021 aacaaactgg agactgtcat ggaaaacctg aatatggatc tgctgtacat ggctgccgct
    10081 gtgatgatgg gtctggcggc aatcggtgct gcgatcggta tcggcatcct cgggggtaaa
    10141 ttcctggaag gcgcagcgcg tcaacctgat ctgattcctc tgctgcgtac tcagttcttt
    10201 atcgttatgg gtctggtgga tgctatcccg atgatcgctg taggtctggg tctgtacgtg
    10261 atgttcgctg tcgcgtagta agcgttgctt ttatttaaag agcaatatca gaacgttaac
    10321 taaatagagg cattgtgctg tgaatcttaa cgcaacaatc ctcggccagg ccatcgcgtt
    10381 tgtcctgttc gttctgttct gcatgaagta cgtatggccg ccattaatgg cagccatcga
    10441 aaaacgtcaa aaagaaattg ctgacggcct tgcttccgca gaacgagcac ataaggacct
    10501 tgaccttgca aaggccagcg cgaccgacca gctgaaaaaa gcgaaagcgg aagcccaggt
    10561 aatcatcgag caggcgaaca aacgccgctc gcagattctg gacgaagcga aagctgaggc
    10621 agaacaggaa cgtactaaaa tcgtggccca ggcgcaggca gaaattgaag ccgagcgtaa
    10681 acgtgcccgt gaagagctgc gtaagcaagt tgctatcctg gctgttgctg gcgccgagaa
    10741 gatcatcgaa cgttccgtgg atgaagctgc taacagcgac atcgtggata aacttgtcgc
    10801 tgaactgtaa ggagggaggg gctgatgtct gaatttatta cggtagctcg cccctacgcc
    10861 aaagcagctt ttgactttgc cgtcgaacac caaagtgtag aacgctggca ggacatgctg
    10921 acgtttgccg ccgaggtaac caaaaacgaa caaatggcag agcttctctc tggcgcgctt
    10981 gcgccagaaa cgctcgccga gtcgtttatc gcagtttgtg gtgagcaact ggacgaaaac
    11041 ggtcagaacc tgattcgggt aatggctgaa aatggtcgtc ttaacgcgct cccggatgtt
    11101 ctggagcagt ttattcacct tcgtgccgtg agtgaggcta ccgctgaggt agacgtcatt
    11161 tccgctgccg cactgagtga acaacagctc gcgaaaattt ctgctgcgat ggaaaaacgt
    11221 ctgtcacgca aagttaagct gaattgcaaa atcgataagt ctgtaatggc tggcgttatc
    11281 atccgagcgg gtgatatggt cattgatggc agcgtacgcg gtcgtcttga gcgccttgca
    11341 gacgtcttgc agtcttaagg ggactggagc atgcaactga attccaccga aatcagcgaa
    11401 ctgatcaagc agcgcattgc tcagttcaat gttgtgagtg aagctcacaa cgaaggtact
    11461 attgtttctg taagtgacgg tgttatccgc attcacggcc tggccgattg tatgcagggt
    11521 gaaatgatct ccctgccggg taaccgttac gctatcgcac tgaacctcga gcgcgactct
    11581 gtaggtgcgg ttgttatggg tccgtacgct gaccttgccg aaggcatgaa agttaagtgt
    11641 actggccgta tcctggaagt tccggttggc cgtggcctgc tgggccgtgt ggttaacact
    11701 ctgggtgcac caatcgacgg taaaggtccg ctggatcacg acggcttctc tgctgtagaa
    11761 gcaatcgctc cgggcgttat cgaacgtcag tccgtagatc agccggtaca gaccggttat
    11821 aaagccgttg actccatgat cccaatcggt cgtggtcagc gtgaattgat catcggtgac
    11881 cgtcagacag gtaaaaccgc actggctatc gatgccatca tcaaccagcg cgattccggt
    11941 atcaaatgta tctatgtcgc tatcggccag aaagcgtcca ccatttctaa cgtggtacgt
    12001 aaactggaag agcacggcgc actggctaac accatcgttg tggtagcaac cgcgtctgaa
    12061 tccgctgcac tgcaatacct ggcaccgtat gccggttgcg caatgggcga atacttccgt
    12121 gaccgcggtg aagatgcgct gatcatttac gatgacctgt ctaaacaggc tgttgcttac
    12181 cgtcagatct ccctgctgct ccgtcgtccg ccaggacgtg aagcattccc gggcgacgtt
    12241 ttctacctcc actctcgtct gctggagcgt gctgcacgtg ttaacgccga atacgttgaa
    12301 gccttcacca aaggtgaagt gaaagggaaa accggttctc tgaccgcact gccgattatc
    12361 gaaactcagg cgggtgacgt ttctgcgttc gttccgacca acgtaatctc cattaccgat
    12421 ggtcagatct tcctggaaac caacctgttc aacgccggta ttcgtcctgc ggttaacccg
    12481 ggtatttccg tatcccgtgt tggtggtgca gcacagacca agatcatgaa aaaactgtcc
    12541 ggtggtatcc gtaccgctct ggcacagtat cgtgaactgg cagcgttctc tcagtttgca
    12601 tccgaccttg acgatgcaac acgtaagcag cttgaccacg gtcagaaagt gaccgaactg
    12661 ctgaaacaga aacagtatgc gccgatgtcc gttgcgcagc agtctctggt tctgttcgca
    12721 gcagaacgtg gttacctggc ggatgttgaa ctgtcgaaaa ttggcagctt cgaagccgct
    12781 ctgctggctt acgtcgaccg tgatcacgct ccgttgatgc aagagatcaa ccagaccggt
    12841 ggctacaacg acgaaatcga aggcaagctg aaaggcatcc tcgattcctt caaagcaacc
    12901 caatcctggt aacgtctgga ggcttgcctt agggcaggcc gcaaggcatt gaggagaagc
    12961 tcatggccgg cgcaaaagag atacgtagta agatcgcaag cgtccagaac acgcaaaaga
    13021 tcactaaagc gatggagatg gtcgccgctt ccaaaatgcg taaatcgcag gatcgcatgg
    13081 cggccagccg tccttatgca gaaaccatgc gcaaagtgat tggtcacctt gcacacggta
    13141 atctggaata taagcaccct tacctggaag accgcgacgt taaacgcgtg ggctacctgg
    13201 tggtgtcgac cgaccgtggt ttgtgcggtg gtttgaacat taacctgttc aaaaaactgc
    13261 tggcggaaat gaagacctgg accgacaaag gcgttcaatg cgacctcgca atgatcggct
    13321 cgaaaggcgt gtcgttcttc aactccgtgg gcggcaatgt tgttgcccag gtcaccggca
    13381 tgggggataa cccttccctg tccgaactga tcggtccggt aaaagtgatg ttgcaggcct
    13441 acgacgaagg ccgtctggac aagctttaca ttgtcagcaa caaatttatt aacaccatgt
    13501 ctcaggttcc gaccatcagc cagctgctgc cgttaccggc atcagatgat gatgatctga
    13561 aacataaatc ctgggattac ctgtacgaac ccgatccgaa ggcgttgctg gataccctgc
    13621 tgcgtcgtta tgtcgaatct caggtttatc agggcgtggt tgaaaacctg gccagcgagc
    13681 aggccgcccg tatggtggcg atgaaagccg cgaccgacaa tggcggcagc ctgattaaag
    13741 agctgcagtt ggtatacaac aaagctcgtc aggccagcat tactcaggaa ctcaccgaga
    13801 tcgtctcggg ggccgccgcg gtttaaacag gttatttcgt agaggattta agatggctac
    13861 tggaaagatt gtccaggtaa tcggcgccgt agttgacgtc gaattccctc aggatgccgt
    13921 accgcgcgtg tacgatgctc ttgaggtgca aaatggtaat gagcgtctgg tgctggaagt
    13981 tcagcagcag ctcggcggcg gtatcgtgcg taccatcgca atgggttcct ccgacggtct
    14041 gcgtcgcggt ctggatgtaa aagacctcga acacccgatc gaagtcccgg taggtaaagc
    14101 gactctgggc cgtatcatga acgtactggg tgaaccggtc gacatgaaag gcgagatcgg
    14161 tgaagaagag cgttgggcga ttcaccgcgc agcaccttcc tacgaagagc tgtcaaactc
    14221 tcaggaactg ctggaaaccg gtatcaaagt tatcgacctg atgtgtccgt tcgctaaggg
    14281 cggtaaagtt ggtctgttcg gtggtgcggg tgtaggtaaa accgtaaaca tgatggagct
    14341 cattcgtaac atcgcgatcg agcactccgg ttactctgtg tttgcgggcg taggtgaacg
    14401 tactcgtgag ggtaacgact tctaccacga aatgaccgac tccaacgtta tcgacaaagt
    14461 atccctggtg tatggccaga tgaacgagcc gccgggaaac cgtctgcgcg ttgctctgac
    14521 cggtctgacc atggctgaga aattccgtga cgaaggtcgt gacgttctgc tgttcgttga
    14581 caacatctat cgttacaccc tggccggtac ggaagtatcc gcactgctgg gccgtatgcc
    14641 ttcagcggta ggttatcagc cgaccctggc ggaagagatg ggcgttctgc aggaacgtat
    14701 cacctccacc aaaactggtt ctatcacctc cgtacaggcg gtatacgtac ctgcggatga
    14761 cttgactgac ccgtctccgg caaccacctt tgcgcacctt gacgcaaccg tggtactgag
    14821 ccgtcagatc gcgtctctgg gtatctaccc ggccgttgac ccgctggact ccaccagccg
    14881 tcagctggac ccgctggtgg ttggtcagga acactacgac accgcgcgtg gcgttcagtc
    14941 catcctgcaa cgttatcagg aactgaaaga catcatcgcc atcctgggta tggatgaact
    15001 gtctgaagaa gacaaactgg tggtagcgcg tgctcgtaag atccagcgct tcctgtccca
    15061 gccgttcttc gtggcagaag tattcaccgg ttctccgggt aaatacgtct ccctgaaaga
    15121 caccatccgt ggctttaaag gcatcatgga aggcgaatac gatcacctgc cggagcaggc
    15181 gttctacatg gtcggttcca tcgaagaagc tgtggaaaaa gccaaaaaac tttaacgcct
    15241 taatcggagg gtgatatggc aatgacttac cacctggacg tcgtcagcgc agagcaacaa
    15301 atgttctctg gtctggtcga gaaaatccag gtaacgggta gcgaaggtga actggggatc
    15361 taccctggcc acgcaccgct gctcaccgcc attaagcctg gtatgattcg catcgtgaaa
    15421 cagcacggtc acgaagagtt tatctatctg tctggcggca ttcttgaagt gcagcctggc
    15481 aacgtgaccg ttctggccga caccgcaatt cgcggccagg atctcgacga agcgcgagcc
    15541 atggaagcga aacgtaaggc tgaagagcac attagcagct ctcacggcga cgtagattac
    15601 gctcaggcgt ctgcggaact ggccaaagcg atcgcgcagc tgcgcgttat cgagttgacc
    15661 aaaaaagcga tgtaacaccg gcttgaaaag cacaaaagcc agtctggaaa caggctggct
    15721 ttttttacgc gttgacccgt cctgaagagc gttcacatag atcctgctga tataaaacac
    15781 ccctgttttc ccgtttattc attgatcgaa ataagagcaa aaacatccac ctgacgctta
    15841 aatgaaggta ctgccttaat tttctgcaga taaaaggcgt gacgatggtc gaaaatggca
    15901 ctttcgtcag cggggataat ccgttattga acaatttatc ctctgtccat ttcacgatga
    15961 aaaaaatgta gttttttcaa ggtgaagcgg tttaaattcg ttctcaaatt acagtcagga
    16021 cgcgtatgtt gaataatgct atgagcgtag tgatccttgc cgcaggcaaa ggcacgcgca
    16081 tgtattccga tcttccgaaa gtgctgcata cccttgccgg gaaagcgatg gttcagcatg
    16141 tcattgatgc tgcgaatgaa ttaggcgcag cgcacgttca cctggtgtac ggtcacggcg
    16201 gcgatctgct taaacaggcg ctgaaagacg acaaccttaa ctgggtgctt caggcagagc
    16261 agctgggtac gggtcatgca atgcagcagg ccgcaccttt ctttgccgat gatgaagaca
    16321 ttttaatgct ctacggcgac gtgccgctga tctctgtcga aacactccag cgcctgcgtg
    16381 atgctaaacc ccagggtggc attggtctgc tgacggtgaa actggatgat ccgaccggtt
    16441 atggacgtat cacccgtgaa aatggcaaag ttaccggcat tgttgagcac aaagatgcca
    16501 ccgacgagca gcgtcagatt caggagatca acaccggcat tctgattgcc aacggcgcag
    16561 atatgaaacg ctggctggcg aagctgacca acaataatgc tcagggcgaa tactacatca
    16621 ccgacattat tgcgctggcg tatcaggaag ggcgtgaaat cgtcgccgtt catccgcaac
    16681 gtttaagcga agtagaaggt gtgaataacc gcctgcaact ctcccgtctg gagcgcgttt
    16741 accagtccga acaggctgaa aaactgctgt tagcaggcgt tatgctgcgc gatccggcgc
    16801 gttttgatct gcgcggtacg cttactcacg ggcgcgatgt tgaaattgat actaacgtta
    16861 tcatcgaggg caacgtgact ctcggtcatc gcgtgaaaat cggcaccggt tgcgtgatta
    16921 aaaacagcgt gattggcgat gattgcgaaa tcagtccgta taccgttgtg gaagacgcga
    16981 atctggcagc ggcctgtact attggcccgt ttgcccgtct gcgtcctggt gctgagttgc
    17041 tggaaggtgc acacgttggt aacttcgttg agatgaaaaa agcgcgtctg ggtaaaggca
    17101 cgaaagccgg gcatctgact tacctgggcg atgcggaaat tggcgataac gttaacatcg
    17161 gcgcgggaac cattacctgc aactacgatg gtgcgaataa atttaagacc attatcggcg
    17221 acgatgtgtt tgtcggttcc gacactcagc tggtggcccc ggtaacagta ggcaaaggcg
    17281 cgaccattgc tgcgggtaca actgtgacgc gtaatgtcgg cgaaaatgca ttagctatca
    17341 gccgtgtgcc gcagactcag aaagaaggct ggcgtcgtcc ggtaaagaaa aagtaattct
    17401 ggccgggtaa tccggtcatc tgggatgagg agataacata atctccctcc cacaagcagt
    17461 aactataaaa ataaccccac tctctacaag gctcggggcg cccgaaaaaa cgggcataca
    17521 ggttgaccga caacgatata aatcggaatc aaaaactatg tgtggaattg ttggcgcgat
    17581 cgcgcaacgt gatgtagcag aaatccttct tgaaggttta cgtcgtctgg aataccgcgg
    17641 atatgactct gccggtctgg ccgttgttga tgcggaaggt catatgactc gcctgcgtcg
    17701 cctcggtaaa gtccagatgc tggctcaggc agcggaagaa catcctctgc atggcggcac
    17761 cggtattgct cacactcgct gggcgaccca cggtgaacct tcagaagcta acgcgcatcc
    17821 gcatgtttct gaacacattg tggtggtgca taatggcatc atcgaaaacc atgaaccgct
    17881 gcgtgaagag ttaaaagcgc gtggctatac cttcgtttct gaaaccgaca ccgaagtgat
    17941 tgcccatctg gtgaactggg agctgaaaca aggcgggact ctgcgtgagg ctgttctgcg
    18001 tgctatcccg cagctgcgcg gtgcgtacgg tacagtgatc atggactccc gtcacccgga
    18061 taccctgcta gcggcacgtt ctggtagtcc gctggtgatt ggcctgggta tgggcgaaaa
    18121 ctttatcgct tctgaccagc tggcgctgtt gccggtgacc cgtcgcttta tcttccttga
    18181 agagggcgat attgcggaaa tcactcgccg ttcggtaaac atcttcgata aaactggcgc
    18241 ggaagtaaaa cgtcaggata tcgaatccaa tctgcaatat gacgcgggcg ataaaggtat
    18301 ttaccgtcac tacatgcaga aagagatcta cgaacagccg aacgcgataa aaaacaccct
    18361 taccgggcgc atcagccacg gtcaggttga tttaagcgag ctgggaccga acgccgacga
    18421 actgctgtcg aaggttgagc atattcagat cctcgcctgt ggtacttctt ataactccgg
    18481 tatggtttcc cgctactggt ttgaatcgct ggcaggtatt ccgtgcgacg tcgaaatcgc
    18541 ctctgaattc cgctatcgta aatctgccgt gcgtcgtaac agcctgatga tcaccttgtc
    18601 acagtctggc gaaaccgcgg ataccctggc gggcctgcgt ctgtcgaaag agctgggtta
    18661 ccttggttcg ctggcaatct gtaacgttcc gggttcttct ctggtgcgcg aatccgatct
    18721 ggcgctgatg accaacgcgg gtacggaaat cggcgtggcg tccactaaag cattcaccac
    18781 tcagttaact gtgctgttga tgctggtggc gaagctgtct cgcctgaaag gtctggatgc
    18841 ctccattgaa catgacattg tgcatggtct gcaggcgctg ccgagccgta ttgagcagat
    18901 gctgtctcag gataaacgca ttgaagctct ggcagaagat ttctctgaca aacatcacgc
    18961 gctgttcctg ggccgtggcg atcagtaccc aatcgcgctg gaaggcgcat tgaagctgaa
    19021 agagatctct tacattcacg ctgaagccta cgctgcaggc gagctgaaac acggcccgct
    19081 ggcgctgatt gacgcggata tgccggttat cgtcgttgca ccgaacaacg aactgttgga
    19141 aaaactgaaa tccaacattg aagaagttcg cgcgcgtggc ggtcaattgt atgtcttcgc
    19201 cgatcaggat gcgggttttg taagtagcga taacatgcac atcatcgaga tgccgcatgt
    19261 ggaagaggtg attgcaccga tcttctacac cgttccgctg cagctgctgg cttaccatgt
    19321 cgcgctgatc aaaggcaccg acgttgacca gccgcgtaac ctggcaaaat cggttacggt
    19381 tgagtaatca ctatcataga taaaaaaggg ctgctgtggg cggacaataa agtcttaaac
    19441 tgaacaaaat agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa
    19501 ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca
    19561 aactcttcat tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg
    19621 catggtaaag actatattcc atggctaaca gtacaagaag ttccttcttc aggtcgttcc
    19681 caccgtattt attctcataa gacgggacga gtccatcatt tgctatctga cttagagctt
    19741 gctgtttttc tcagtcttga gtgggagagc agcgtgctag atatacgcga gcagttcccc
    19801 ttattaccta gtgataccag gcagattgca atagatagtg gtattaagca tcctgttatt
    19861 cgtggtgtag atcaggttat gtctactgat tttttagtgg actgcaaaga tggtcctttt
    19921 gagcagtttg ctattcaagt caaacctgca gcagccttac aagacgagcg taccttagaa
    19981 aaactagaac tagagcgtcg ctattggcag caaaagcaaa ttccttggtt catttttact
    20041 gataaagaaa taaatcccgt agtaaaagaa aatattgaat ggctttattc agtgaaaaca
    20101 gaagaagttt ctgcggagct tttagcacaa ctatccccat tggcccatat cctgcaagaa
    20161 aaaggagatg aaaacattat caatgtctgt aagcaggttg atattgctta tgatttggag
    20221 ttaggcaaaa cattgagtga gatacgagcc ttaaccgcaa atggttttat taagttcaat
    20281 atttataagt ctttcagggc aaataagtgt gcagatctct gtattagcca agtagtgaat
    20341 atggaggagt tgcgctatgt ggcaaattaa tgaggttgtg ctatttgata atgatccgta
    20401 tcgcattttg gctatagagg atggccaagt tgtctggatg caaataagcg ctgataaagg
    20461 agttccacaa gctagggctg agttgttgct aatgcagtat ttagatgaag gccgcttagt
    20521 tagaactgat gacccttatg tacatcttga tttagaagag ccgtctgtag attctgtcag
    20581 cttccagaag cgcgaggagg attatcgaaa aattcttcct attattaata gtaaggatcg
    20641 tttcgaccct aaagtcagaa gcgaactcgt tgagcatgtg gtccaagaac ataaggttac
    20701 taaggctaca gtttataagt tgttacgccg ttactggcag cgtggtcaaa cgcctaatgc
    20761 attaattcct gactacaaaa acagcggtgc accaggggaa agacgttcag cgacaggaac
    20821 agcaaagatt ggccgagcca gagaatatgg taagggtgaa ggaaccaagg taacgcccga
    20881 gattgaacgc ctttttaggt tgaccataga aaagcacctg ttaaatcaaa aaggtacaaa
    20941 gaccaccgtt gcctatagac gatttgtgga cttgtttgct cagtattttc ctcgcattcc
    21001 ccaagaggat tacccaacac tacgtcagtt tcgttatttt tatgatcgag aataccctaa
    21061 agctcagcgc ttaaagtcta gagttaaagc aggggtatat aaaaaagacg tacgaccctt
    21121 aagtagtaca gccacttctc aggcgttagg ccctgggagt cgttatgaga ttgatgccac
    21181 gattgctgat atttatttag tggatcatca tgatcgccaa aaaatcatag gaagaccaac
    21241 gctttacatt gtgattgatg tgtttagtcg gatgatcacg ggcttttata tcggctttga
    21301 aaatccgtct tatgtggtgg cgatgcaggc ttttgtaaat gcttgctctg acaaaacggc
    21361 catttgtgcc cagcatgata ttgagattag tagctcagac tggccgtgtg taggtttgcc
    21421 agatgtgttg ctagcggacc gtggcgaatt aatgagtcat caggtcgaag ccttagtttc
    21481 tagttttaat gtgcgagtgg aaagtgctcc acctagacgt ggcgatgcta aaggcatagt
    21541 ggaaagcact tttagaacac tacaagccga gtttaagtcc tttgcacctg gcattgtaga
    21601 gggcagtcgg atcaaaagcc atggtgaaac agactatagg ttagatgcat ctctgtcggt
    21661 atttgagttc acacaaatta ttttgcgtac gatcttattc agaaataacc atctggtgat
    21721 ggataaatac gatcgagatg ctgattttcc tacagattta ccgtctattc ctgtccagct
    21781 atggcaatgg ggtatgcagc atcgtacagg tagtttaagg gctgtggagc aagagcagtt
    21841 gcgagtagcg ttactgcctc gccgaaaggt ctctatttct tcatttggcg ttaatttgtg
    21901 gggtttgtat tactcggggt cagagattct gcgtgagggt tggttgcagc ggagcactga
    21961 tatagctaga cctcaacatt tagaagcggc ttatgaccca gtgctggttg atacgattta
    22021 tttgtttccg caagttggca gccgtgtatt ttggcgctgt aatctgacgg aacgtagtcg
    22081 gcagtttaaa ggtctctcat tttgggaggt ttgggatata caagcacaag aaaaacacaa
    22141 taaagccaat gcgaagcagg atgagttaac taaacgcagg gagcttgagg cgtttattca
    22201 gcaaaccatt cagaaagcga ataagttaac gcccagtact actgagccca aatcaacacg
    22261 cattaagcag attaaaacta ataaaaaaga agccgtgacc tcggagcgta aaaaacgtgc
    22321 ggagcatttg aagccaagct cttcaggtga tgaggctaaa gttattcctt tcaacgcagt
    22381 ggaagcggat gatcaagaag attacagcct acccacatac gtgcctgaat tatttcagga
    22441 tccaccagaa aaggatgagt catgagtgct acccggattc aagcagttta tcgtgatacg
    22501 ggggtagagg cttatcgtga taatcctttt atcgaggcct taccaccatt acaagagtca
    22561 gtgaatagtg ctgcatcact gaaatcctct ttacagctta cttcctctga cttgcaaaag
    22621 tcccgtgtta tcagagctca taccatttgt cgtattccag atgactattt tcagccatta
    22681 ggtacgcatt tgctactaag tgagcgtatt tcggtcatga ttcgaggtgg ctacgtaggc
    22741 agaaatccta aaacaggaga tttacaaaag catttacaaa atggttatga gcgtgttcaa
    22801 acgggagagt tggagacatt tcgctttgag gaggcacgat ctacggcaca aagcttattg
    22861 ttaattggtt gttctggtag tgggaagacg acctctcttc atcgtattct agccacgtat
    22921 cctcaggtga tttaccatcg tgaactcaat gtagagcagg tggtgtattt gaaaatagac
    22981 tgctcgcata atggttcgct aaaagaaatc tgcttgaatt ttttcagagc gttggatcga
    23041 gccttgggct cgaactatga gcgtcgttat ggcttaaaac gtcatggtat agaaaccatg
    23101 ttggctttga tgtcgcaaat agccaatgca catgctttag ggttgttggt tattgatgaa
    23161 attcagcatt taagccgctc tcgttcgggt ggatctcaag agatgctgaa cttttttgtg
    23221 acgatggtga atattattgg cgtaccagtg atgttgattg gtacccctaa agcacgagag
    23281 atttttgagg ctgatttgcg gtctgcacgt agaggggcag ggtttggagc tatattctgg
    23341 gatcctatac aacaaacgca acgtggaaag cccaatcaag agtggatcgc ttttacggat
    23401 aatctttggc aattacagct tttacaacgc aaagatgcgc tgttatcgga tgaggtccgt
    23461 gatgtgtggt atgagctaag ccaaggagtg atggacattg tagtaaaact ttttgtactc
    23521 gctcagctcc gtgcgctagc tttaggcaat gagcgtatta ccgctggttt attgcggcaa
    23581 gtgtatcaag atgagttaaa gcctgtgcac cccatgctag aggcattacg ctcgggtatc
    23641 ccagaacgca ttgctcgtta ttctgatcta gtcgttcccg agattgataa acggttaatc
    23701 caacttcagc tagatatcgc agcgatacaa gaacaaacac cagaagaaaa agcccttcaa
    23761 gagttagata ccgaagatca gcgtcattta tatctgatgc tgaaagagga ttacgattca
    23821 agcctgttaa ttcccactat taaaaaagcg tttagccaga atccaacgat gacaagacaa
    23881 aagttactgc ctcttgtttt gcagtggttg atggaaggcg aaacggtagt gtcagaacta
    23941 gaaaagccct ccaagagtaa aaaggtttcg gctataaagg tagtcaagcc cagcgactgg
    24001 gatagcttgc ctgatacgga tttacgttat atctattcac aacgccaacc tgaaaaaacc
    24061 atgcatgaac ggttaaaagg gaaaggggta atagtggata tggcgagctt atttaaacaa
    24121 gcaggttagc catgagaaac tttcctgttc cgtactcgaa tgagctgatt tatagcacta
    24181 ttgcacgggc aggcgtttat caagggattg ttagtcctaa gcagctgttg gatgaggtgt
    24241 atggcaaccg caaggtggtc gctaccttag gtctgccctc gcatttaggt gtgatagcaa
    24301 gacatctaca tcaaacagga cgttacgctg ttcagcagct tatttatgag cataccttat
    24361 tccctttata tgctccgttt gtaggcaagg agcgccgaga cgaagctatt cggttaatgg
    24421 agtaccaagc gcaaggtgcg gtgcatttaa tgctaggagt cgctgcttct agagttaaga
    24481 gcgataaccg ctttagatac tgccctgatt gcgttgctct tcagctaaat aggtatgggg
    24541 aagccttttg gcaacgagat tggtatttgc ccgctttgcc atattgtcca aaacacggtg
    24601 ctttagtctt ctttgataga gctgtagatg atcaccgaca tcaattttgg gctttgggtc
    24661 atactgagct gctttcagac taccccaaag actccctatc tcaattaaca gcactagctg
    24721 cttatatagc ccctctgtta gatgctccac gagcgcaaga gctttcccca agccttgagc
    24781 agtggacgct gttttatcag cgcttagcgc aggatctagg gctaaccaaa agcaagcaca
    24841 ttcgtcatga cttggtggcg gagagagtga ggcagacttt tagtgatgag gcactagaga
    24901 aactggattt aaagttggca gagaacaagg acacgtgttg gctgaaaagt atattccgta
    24961 agcatagaaa agcctttagt tatttacagc atagtattgt gtggcaagcc ttattgccaa
    25021 aactaacggt tatagaagcg ctacagcagg caagtgctct tactgagcac tctataacga
    25081 caagacctgt tagccagtct gtgcaaccta actctgaaga tttatctgtt aagcataaag
    25141 actggcagca actagtgcat aaataccaag gaattaaggc ggcaagacag tctttagagg
    25201 gtggggtgct atacgcttgg ctttaccgac atgacaggga ttggctagtt cactggaatc
    25261 aacagcatca acaagagcgt ctggcacccg cccctagagt tgattggaac caaagagatc
    25321 gaattgctgt acgacaacta ttaagaatca taaagcgtct agatagtagc cttgatcacc
    25381 caagagcgac atcgagctgg ctgttaaagc aaactcctaa cggaacctct cttgcaaaaa
    25441 atctacagaa actgcctttg gtagcgcttt gcttaaagcg ttactcagag agtgtggaag
    25501 attatcaaat tagacggatt agccaagctt ttattaagct taaacaggaa gatgttgagc
    25561 ttaggcgctg gcgattatta agaagtgcaa cgttatctaa agagcggata actgaggaag
    25621 cacaaagatt cttggaaatg gtttatgggg aagagtgagt ggttaggcta gctacattta
    25681 atgacaatgt gcaggttgta catattggtc atttattccg taactcgggt cataaggagt
    25741 ggcgtatttt tgtttggttt aatccaatgc aagaacggaa atggactcga tttactcatt
    25801 tgcctttatt aagtcgagct aaggtggtta acagtacaac aaagcaaata aataaggcgg
    25861 atcgtgtgat tgagtttgaa gcatcggatc ttcaacgagc caaaataatc gattttccta
    25921 atctctcgtc ctttgcttcc gtacgcaaca aggatggagc gcagagttca tttatttacg
    25981 aagctgaaac accatatagc aagactcgtt atcacatccc acagttagag ctagctcggt
    26041 cattattttt aattaactcc tatttctgtc gaagctgttt gagcagtacc gctttacagc
    26101 aagagttcga cgttcagtat gaggttgagc gagatcattt agagataagg atcttaccca
    26161 gttcatcgtt tcctaaaggg gcgttagagc tgtcggccgt agtgcagctt ttggtttggt
    26221 tgttttcgga tcaagatgtt atggattcgt atgaaagtat ttttaggcac tatcaacaaa
    26281 atagagaaat taagaacggc gttgaaagct ggtgctttag ctttgaccct ccgcccatgc
    26341 agggttggaa attacatgta aaaggacgtt cttctaacga ggataaggat tatttagttg
    26401 aggaaatagt aggtttagaa atcaacgcta tgcttcctag cacaacagct attagccatg
    26461 cctcttttca ggaaaaggag gcaggtgatg gtagtacgca gcacatagcg gtttcaacag
    26521 agtcagttgt tgatgatgag catctacagt tggacgatga ggaaacagcc aatatagaca
    26581 cagacacacg agtcatagag gctgagccga catggataag ttttagtaga cctagtcgaa
    26641 ttgaaaaatc tcgcagggca agaaaaagta gccaaactat tttagaaaaa gaagaagcaa
    26701 caacaagtga aaatagtaat ttggttagta ctgatgagcc acacttaggt ggtgtcctag
    26761 cagcggcaga tgtgggtggg aagcaggatg caaccaatta caactctatt tttgctaatc
    26821 gatttgctgc ttttgatgag ctactttcaa ttctaaaaac taaatttgca tgtcgggtgc
    26881 tttttgaaga aaccttggtt ttgccaaaag ttgggcgtag ccgattacat ctgtgtaaag
    26941 atggctcacc aagagtgatt aaagccgttg gggtgcaacg taatggcagt gaatttgtat
    27001 tgctagaggt ggatgcatcg gatggggtga aaatgctttc taccaaagtg ttgagtggcg
    27061 ttgatagcga aacatggcgg aatgattttg aaaagatacg gcgtggagtg gtgaagagct
    27121 cattgaattg gccaaatagt ttgtttgatc aattatatgg acaagacggg catagagggg
    27181 tgaatcatcc aaaggggttg ggggagctgc aagtatcgag agaggatatg gaagggtggg
    27241 ctgagagagt ggttagagag caatttacgc attaaaggaa tgactgaaag agcctgtaaa
    27301 cccttttgtg taagtgcttt tgccggtcag ttaaaggtgg ccatttaaac ggtcaccaaa
    27361 ttcgatcata aaacggttca tggccggctt ccagttgcgg atcggcatcg tccatttctt
    27421 ggtcgccgcc tggatagcca ggtacaccac cttcatcgct gattcgtccg tagggaacac
    27481 cttgagttca tattaatgga attttctaca aatagcctcc gtggttttga ggggggatta
    27541 cagacgatcc atagtagtaa tccaatgagt tcttgagcgc ggcgacatgt ttggacgcct
    27601 tggcaaaaat tagagcctgc ttgaagtgca ggcgagcacg tgcttggacg attgatccat
    27661 tcgcggtcaa aaactcaatc ttggatgaca gcgtgtcagg aaatccaata tcgaagtccc
    27721 gccccgtgtc aaagtagggc atttcatgat caaaggacga agcttcccag gccacttccc
    27781 taatattcca gccgctagaa acaagatttc gtacgacttc atcaactttt ttgctttcca
    27841 acttgtcaca aagtgcatag gcaagagctt tggcggcaga cacgtgcgtt tcctcttaaa
    27901 cataggtcga acggataccc gaggcaaagc ggagcattag tcccaaagca tctccggtag
    27961 atccggaggc tccatactga aggcatggca tcttgggcat tgatatttga aacttctctc
    28021 tagatagcag ggatttcttg gccttgcctc atgctgatag cttgtaaata tcacacgatc
    28081 cttagcccca caaagtgagc atactccgta gctttcattt atagaaatcg tcgttagatt
    28141 gtcgcagcat gtgcacgttc cgactatttg ctcaggaagc atc
//
LOCUS       DAZOYF010000037        28068 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZOYF010000037 DAZOYF010000000
VERSION     DAZOYF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.037"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..822
                     /locus_tag="ACHUOF_001522"
     CDS             <1..822
                     /locus_tag="ACHUOF_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0610756.1"
                     /translation="RDNVLESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIID
                     LLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYK
                     IMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAEIINKKHHAIINGKKFKTS
                     RAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVLGL
                     NARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1012..1125
                     /gene="yhiY"
                     /locus_tag="ACHUOF_001523"
     CDS             1012..1125
                     /gene="yhiY"
                     /locus_tag="ACHUOF_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR0610757.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1188..2255
                     /locus_tag="ACHUOF_001524"
     CDS             1188..2255
                     /locus_tag="ACHUOF_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0610758.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2252..4987
                     /gene="rbbA"
                     /locus_tag="ACHUOF_001525"
     CDS             2252..4987
                     /gene="rbbA"
                     /locus_tag="ACHUOF_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR0610759.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4987..6111
                     /locus_tag="ACHUOF_001526"
     CDS             4987..6111
                     /locus_tag="ACHUOF_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0610760.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6170..6445
                     /locus_tag="ACHUOF_001527"
     CDS             6170..6445
                     /locus_tag="ACHUOF_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR0610761.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            6442..6801
                     /locus_tag="ACHUOF_001528"
     CDS             6442..6801
                     /locus_tag="ACHUOF_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR0610762.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6921..7322)
                     /gene="nikR"
                     /locus_tag="ACHUOF_001529"
     CDS             complement(6921..7322)
                     /gene="nikR"
                     /locus_tag="ACHUOF_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR0610763.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7328..8134)
                     /gene="nikE"
                     /locus_tag="ACHUOF_001530"
     CDS             complement(7328..8134)
                     /gene="nikE"
                     /locus_tag="ACHUOF_001530"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR0610764.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(8131..8895)
                     /gene="nikD"
                     /locus_tag="ACHUOF_001531"
     CDS             complement(8131..8895)
                     /gene="nikD"
                     /locus_tag="ACHUOF_001531"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR0610765.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8895..9728)
                     /gene="nikC"
                     /locus_tag="ACHUOF_001532"
     CDS             complement(8895..9728)
                     /gene="nikC"
                     /locus_tag="ACHUOF_001532"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR0610766.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9725..10669)
                     /gene="nikB"
                     /locus_tag="ACHUOF_001533"
     CDS             complement(9725..10669)
                     /gene="nikB"
                     /locus_tag="ACHUOF_001533"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR0610767.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10669..12243)
                     /gene="nikA"
                     /locus_tag="ACHUOF_001534"
     CDS             complement(10669..12243)
                     /gene="nikA"
                     /locus_tag="ACHUOF_001534"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0610768.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12354..12941)
                     /gene="acpT"
                     /locus_tag="ACHUOF_001535"
     CDS             complement(12354..12941)
                     /gene="acpT"
                     /locus_tag="ACHUOF_001535"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR0610769.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12996..14045)
                     /locus_tag="ACHUOF_001536"
     CDS             complement(12996..14045)
                     /locus_tag="ACHUOF_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0610770.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14179..15396
                     /locus_tag="ACHUOF_001537"
     CDS             14179..15396
                     /locus_tag="ACHUOF_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0610771.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15400..15957)
                     /gene="dcrB"
                     /locus_tag="ACHUOF_001538"
     CDS             complement(15400..15957)
                     /gene="dcrB"
                     /locus_tag="ACHUOF_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR0610772.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16030..16695)
                     /locus_tag="ACHUOF_001539"
     CDS             complement(16030..16695)
                     /locus_tag="ACHUOF_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR0610773.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16916..17161
                     /gene="tusA"
                     /locus_tag="ACHUOF_001540"
     CDS             16916..17161
                     /gene="tusA"
                     /locus_tag="ACHUOF_001540"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR0610774.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17263..19461)
                     /gene="zntA"
                     /locus_tag="ACHUOF_001541"
     CDS             complement(17263..19461)
                     /gene="zntA"
                     /locus_tag="ACHUOF_001541"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR0610775.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19535..20161)
                     /locus_tag="ACHUOF_001542"
     CDS             complement(19535..20161)
                     /locus_tag="ACHUOF_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR0610776.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20302..20661
                     /locus_tag="ACHUOF_001543"
     CDS             20302..20661
                     /locus_tag="ACHUOF_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR0610777.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20664..20933)
                     /locus_tag="ACHUOF_001544"
     CDS             complement(20664..20933)
                     /locus_tag="ACHUOF_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR0610778.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20923..21519)
                     /gene="rsmD"
                     /locus_tag="ACHUOF_001545"
     CDS             complement(20923..21519)
                     /gene="rsmD"
                     /locus_tag="ACHUOF_001545"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR0610779.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            21669..23165
                     /gene="ftsY"
                     /locus_tag="ACHUOF_001546"
     CDS             21669..23165
                     /gene="ftsY"
                     /locus_tag="ACHUOF_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR0610780.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23168..23836
                     /gene="ftsE"
                     /locus_tag="ACHUOF_001547"
     CDS             23168..23836
                     /gene="ftsE"
                     /locus_tag="ACHUOF_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR0610781.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23829..24887
                     /gene="ftsX"
                     /locus_tag="ACHUOF_001548"
     CDS             23829..24887
                     /gene="ftsX"
                     /locus_tag="ACHUOF_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR0610782.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25132..25986
                     /gene="rpoH"
                     /locus_tag="ACHUOF_001549"
     CDS             25132..25986
                     /gene="rpoH"
                     /locus_tag="ACHUOF_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR0610783.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26257..27360
                     /gene="livJ"
                     /locus_tag="ACHUOF_001550"
     CDS             26257..27360
                     /gene="livJ"
                     /locus_tag="ACHUOF_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR0610784.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27559..27942)
                     /gene="panM"
                     /locus_tag="ACHUOF_001551"
     CDS             complement(27559..27942)
                     /gene="panM"
                     /locus_tag="ACHUOF_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR0610785.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6678 a         7257 c         7764 g         6369 t
ORIGIN      
        1 agagataatg ttttagaaag tcttcagcct tatttggtta ttaataaaga cgcagtcacg
       61 ccatgtacgt ttcttggcga ccaaaccggc gatcgattta gtaatatctg tggcgatcaa
      121 tttatcatcg atttgttaaa acgcataatg agtatcaatg aaaatgttca tgtattagct
      181 ggaaatcatg aaacgaattg caatggtaac tatatgcaaa acttcacgcg catgaaacca
      241 cttgatgagg atacctacga cgggataaaa gattatcctg tatgtttcta tgaccccaaa
      301 tacaaaataa tggcaaatca tcatgggata acttttgatg atcagcgaaa acgttacatt
      361 ataggaccga taactgtctc tattgatgaa atgaccaatg ctctcgatcc ggtcgaatta
      421 gcggaaatta ttaataaaaa gcatcatgct ataattaatg gcaaaaagtt taaaactagc
      481 cgggctatct cttgtcgatc ctttaatcgt tatttttctg tttcgacaga ttacagacct
      541 aaacttgaag cactattagc atgttcacaa atgttaggta taaatcaagt tgttgcacat
      601 aacggaaatg gtgggagaga acgcataggt gaaacgggga cagttttagg tcttaacgcc
      661 cgtgacagta aacatgctgg aagaatgttc agcatgcata attgccaaat taaccctggt
      721 gcagggccag agataaccac tccctggaag tcttaccagc atgaaaaaaa cagaaatgga
      781 cttatgcctt tgattagaag gcgcacaatg ttacagctct gaaccataga caacatgaat
      841 attaatgtta ctatctggtt gagactgttg ataaaacgta aaaaggaatg ctctgttcct
      901 gcaactgaac aggcgtgaaa tgcgatccaa caggatggat accgttttgt cagccagttt
      961 tatagatcac acttatgagt atgcgtgttt catttacggc caggaatgct gatgatgaca
     1021 acattactac ccgttttcac taagccttcc ccgttggcgc tcaatgctct gcgcgctggt
     1081 cgtatttgtc gtttccttct tatcccggat ggaagaatcc gctgaacgtc gttcggcggc
     1141 agttgtaaca cgcgtcgttt gcttacaggg attatggcgg gtcgattatg gataagagta
     1201 agcgccatct ggcgtggtgg gttgtcgggg cactggcggt ggctgccgtc gtggcgtggt
     1261 ggctgttgcg cccggcgggt gtgccggaag gctttgctgt cagcaatggg cgcattgaag
     1321 cgacggaagt ggatatcgcc agcaaaattg ccgggcgtat cgacaccatt ctggtgaaag
     1381 aaggccagtt tgttcgcgaa ggcgaagtgc tggcgaagat ggatactcgc gtgttgcagg
     1441 aacagcgact ggaagccatc gcgcaaatca aagaggcaca aagcgccgtt gccgccgcgc
     1501 aggctttgct ggagcaacga caaagtgaaa ctcgtgccgc acagtcgctg gtcaatcaac
     1561 gccaggccga actggactct gttgccaaac gtcatacgcg ttcccgctcg ctcgcccaac
     1621 gaggggctat ttctgcgcaa cagctggatg acgaccgcgc cgccgctgag agcgcccgag
     1681 ctgcgctgga atcagcgaaa gcccaggttt cggcttctaa agcggctata gaagcagcac
     1741 gcaccaatat cattcaggcg caaactcggg tcgaagcagc gcaagccact gaacggcgca
     1801 ttgccgcaga tatcgatgac agtgaactga aagccccgcg tgacggacgc gtgcagtatc
     1861 gggttgccga gccaggggaa gtgctggcgg caggcggtcg ggtgctgaat atggtcgatc
     1921 tcagcgacgt ctatatgacc ttcttcctgc caaccgaaca ggcgggcacg ctgaaactgg
     1981 gcggtgaagc ccggctgatc ctcgatgccg cgccagatct gcgtattcct gcaaccatca
     2041 gttttgtcgc cagtgtcgcc cagttcacgc caaaaaccgt cgaaaccagc gatgaacggc
     2101 tgaaactgat gttccgcgtc aaagcgcgta tcccaccgga attactccag cagcatctgg
     2161 aatatgtcaa aaccggttta ccgggcgtag cgtgggtgcg ggtgaatgaa gaacttccgt
     2221 ggcctgacga cctcgtggtg aggttgccgc aatgacgcat ctggaactgg ttcccgtccc
     2281 gcctgtcgcg caactggcgg gcgtgagcca gcattatgga aaaaccgttg cgctgaacaa
     2341 tatcactctc gatattcagg cccgttgcat ggtcgggctg attggtccgg atggcgtcgg
     2401 gaagtcgagt ttattgtcac tgatttccgg tgcccgcgtc attgagcagg gcaatgtggt
     2461 ggtgctgggt ggcgatatgc acgacccgaa gcatcgccga gacgtctgcc cgcgcatcgc
     2521 ctggatgccg caggggctgg gcaaaaacct ctaccacacc ttgtcggtgt atgaaaatgt
     2581 cgattttttc gctcgcctgt tcggtcacga taaagcggag cgggaagcgc gaattaatga
     2641 gctgctgacc agcaccgggt tagcaccgtt tcgcgatcgt ccggcaggta aactctccgg
     2701 cgggatgaag caaaaacttg ggctgtgctg cgcgttaatc cacgacccgg aactgttgat
     2761 ccttgatgag ccaacaacgg gggttgaccc gctctcccgc gcccagttct gggatctgat
     2821 cgacagtatt cgccagcggc agagcaatat gagcgtgctg gtcgccaccg cctatatgga
     2881 agaggccgaa cgcttcgact ggctggtagc gatgaatgcc ggagaagtgc tggcaaccgg
     2941 cagcgccgaa gagctgcggc agcaaacgca aagcgccacg ctggaagaag catttattaa
     3001 tctgttaccg caagcgcaac gccaggcgca tcaggcggta gtgatcccac cgtatcaacc
     3061 tgaaaacgca gagattgcca tcgaagcgcg cgatctgacc atgcgttttg gttccttcgt
     3121 tgccgttgat cacgtcaatt tccgcattcc acgcggggag atttttggat tccttggttc
     3181 gaacggctgc ggtaaatcca ccaccatgaa aatgcttacc ggactgctgc ccgccagcga
     3241 aggtgaggcg tggctatttg ggcaaccggt tgatccaaaa gatatcgata cccgccgtcg
     3301 tgtgggctat atgtcgcagg cgttttcgct ctataacgaa ctcaccgtgc ggcaaaacct
     3361 ggagttacat gcccgtttgt ttcacatccc ggaagcggaa attcccgcgc gagtggctga
     3421 aatgagcgag cgttttaagc tcaacgacgt tgaagatgtt ctgccggagt cattgccgct
     3481 cggcattcgc cagcggcttt cgctggcggt ggcggtgatt catcgcccgg agatgttaat
     3541 cctcgatgag cctacttctg gtgtcgatcc ggtggcgagg gatatgttct ggcagttgat
     3601 ggtcgatctc tcgcgccagg acaaagtgac tatcttcatc tccacccact ttatgaacga
     3661 agcggaacgt tgcgaccgca tctcactgat gcacgccgga aaagtgcttg ccagcggtac
     3721 accgcaggaa ctggttgaga aacgcggagc cgccagtctg gaagaggcat ttatcgccta
     3781 tttgcaggaa gcggcagggc agagcaacga agccgaagcg ccgcccgtgg tacacgacac
     3841 cacccacgcg ccgcgtcagg gatttagcct gcgccgtctg tttagctaca gccgccgcga
     3901 agcgctggaa ctgcgacgcg atccagtacg ttcgacgctg gcgctgatgg gaacggtgat
     3961 cctgatgctg ataatgggtt acggcatcag tatggatgtg gaaaacctgc gctttgcggt
     4021 gctcgaccgc gaccagaccg tcagtagcca ggcgtggaca ctcaacctct ccggttcccg
     4081 ttactttatc gaacagccgc cgctcaccag ttatgacgag cttgatcgtc ggatgcgtgc
     4141 gggcgatatc acggtggcca ttgagatccc gcccaatttc gggcgcgata tcgcgcgtgg
     4201 tacgcctgtg gaactcggcg tctggatcga cggagcgatg cccagtcgtg ccgaaacggt
     4261 aaaaggttac gtgcaggcaa tgcaccagag ttggcttcag gatgtggcga gccgacaatc
     4321 gacacccgcc agccaaagcg ggctgatgaa cattgagacg cgttatcgct ataacccgga
     4381 cgtaaaaagc ctgccagcga ttgttccggc ggtgatcccg cttctgctga tgatgatccc
     4441 gtcaatgcta agcgccctta gcgtggtgcg ggaaaaagag ctagggtcga ttatcaacct
     4501 ttacgtgacc cccaccaccc gtagcgaatt tttgcttggc aaacagctgc cgtacatcgc
     4561 gctggggatg ctgaactttt tcctgctctg cggcctgtcg gtgtttgtgt ttggcgtacc
     4621 gcataaaggc agtttcctga cgctcacctt ggcggcgctg ctgtatatca tcattgccac
     4681 tggaatgggg ctgctgatct ccacctttat gaaaagccag attgccgcca ttttcggaac
     4741 ggcgattatc acattgatcc cggcgacaca gttttccggg atgatcgatc cggtagcttc
     4801 gctggaaggg cctggacgtt ggatcggcga ggtttacccg accagtcatt ttctgactat
     4861 cgcccgcgga acgttctcga aagcgctgga tctgactgat ttgtggcaac tttttatccc
     4921 gttgctgata gccatcccgc tggtgatggg cttaagcatc ctgctgctga aaaaacagga
     4981 gggatgatgc gccatttacg caatattttt aatctgggta tcaaagagtt gcgcagtctg
     5041 ctcggtgata aagcgatgct gacgctgatt gtcttctcgt ttacggtgtc ggtgtattcg
     5101 tcagcgaccg ttacgccagg atcgttgaac ctcgcgccga tcgccattgc cgatatggat
     5161 caatcgcagt tatcgaaccg gatcgttaac agcttctatc gtccgtggtt tttgccaccg
     5221 gagatgatca ccgccgatga gatggatgcc ggactggacg ccggacgcta taccttcgcg
     5281 ataaatattc cgcctaattt tcagcgtgat gtcctcgccg gacgccagcc ggatattcag
     5341 gtgaacgtcg atgccacgcg catgagccag gcatttaccg gcaatgggta tatccagaat
     5401 attatcaacg gtgaagtgaa cagctttgtc gcgcgctacc gtgataacag cgaaccgttg
     5461 gtatcgctgg aaacccggat gcgctttaac ccgaaccttg atcccgcgtg gtttggcggg
     5521 gtgatggcga tcatcaacaa cattaccatg ctggcgattg tattgaccgg atcggcgctg
     5581 atccgcgagc gtgaacacgg cacggtggaa catttactgg tgatgccgat aacgccgttt
     5641 gagatcatga tggcgaagat ctggtcgatg gggctggtgg tgctggtggt atcgggatta
     5701 tcgctggtgc tgatggtgaa aggtgtactg ggcgtaccga ttgaaggctc gatcccgctg
     5761 tttatgctgg gcgtggcgct cagtctgttt gccaccacgt caatcggcat ttttatgggg
     5821 acgatagcgc gttcaatgcc gcaactgggg ctgctggtga ttctggtgct gctgccgctg
     5881 caaatgcttt ccggtggatc cacgccgcgc gaaagtatgc cgcagatggt gcaggacatt
     5941 atgctgacca tgccgacgac acactttgtt agcctcgcgc aggccatcct ctaccggggt
     6001 gccggattcg aaatcgtctg gccgcagttc ctgacgctga tggcaattgg cggcgcgttt
     6061 ttcaccatcg cactgctgcg attcaggaag acgattggga caatggcgta aggaagggaa
     6121 acttgacttc atactgtgat gtcataaagt gaagtcataa ggacatgcca tgagaaccaa
     6181 ggtacaggct ttacggaaga aacaaaaaaa tacattggat caaatattta aaactcctgt
     6241 tcctcaaggg atcaagtggt ctgatataga gtcactggtt aaagcattag gcggagaaat
     6301 taaggaagga agaggttcgc gttgcaagtt catactaaat atgagcgttg cgtgtttcca
     6361 tcggcctcat ccgtcgccag ataccgataa aggcgctgta gaaagcgtgt gtgactggtt
     6421 actaagtata ggggtaaaac catgatcaaa ctgaagacgc caaactctat ggagattgcc
     6481 gggcaaccag ctgttattac ctacgtgcca gaactgaatg catttcgtgg caaattcctg
     6541 ggcttgtcag gttattgtga ttttgtctct gacagtattc aggggctgca aaaagaagga
     6601 gagctttccc tgcgagagta tcttgaggat tgtaaagctg cgggtattga gccgtatgcg
     6661 cgtacagaaa agattaagac ctttacgtta cgctatccag aatcgctgag tgaacgcctt
     6721 aataatgcgg ctgcgcagca acaagtctct gttaatacct atattattga aacactgaat
     6781 gagcgtttga atcatttata aattttagcg acgtacatta cgcagaatat ctgcctttac
     6841 aggatctatc tatgcaactg aacttaattt tataaattaa gttcagttaa tatcttttct
     6901 ttccttgttt tagaaataac tcaatcttcc ttcggcaggc actgcaaatg cccgtgccgc
     6961 acgccgcgct gggcgataac gtcatcggca aaatgctgca cgtcacccat gtcacctttc
     7021 aacacggcga tttccagaca gtcgtcgtgg ttgatgtgca catgtagcgt ggcgacggag
     7081 aggtcgtggt gatgatgctg ggtggaaaca atacggctgg ctaagtcgcg tttttcgtgt
     7141 tcatacacat acgacagcac cgcgaaacct tgtgtgccgt gctgctgggt agcttcctgc
     7201 gccagggcac tacgcagaat atcgcggata gcttcggaac ggttgttata accacgacgc
     7261 tggctcaggc tgtccagcgt ctccagtaaa tcgtcatcaa gcgtgatggt gactcgttgc
     7321 attggcgtta aaccttttct gaggtgcgac ggcgcacggg gaatgcgggt aataccgcgt
     7381 tttgtagcac acgtccggcg tcagaggaaa aggttaattt atctcccacc gcctgggttt
     7441 cggcgatttg tccgttgtcc atcaccatca ctcgctggca aaagcgttcc accaggcgta
     7501 agtcatgagt gatgaacagg caggcagtgc caaactgttg ttgtagcttt ttcagcaggc
     7561 gaatgacgcc cgcctgtaac acgagatcaa ggttagaaac ggcttcatcc agaatcagca
     7621 atttcggttc gaccgccagc gcgcgtgcca ggcagacgag ctggagctgg ccgccgctta
     7681 actgcggtgg gcgtttgtcg agaacgctgt catcgagatc gaccgccttc agcatttcgc
     7741 tggcgcgcgc cagttgttcg gattttttca gtgatagcag gtggcgcatc ggttcacgca
     7801 ggatctcgcg cacggttttg cgcggattca cggcactgat ggagtcctga aacaccatct
     7861 gaatatcacg gcggaaggct ttacgttggg cgcgattaag tttcgccagc gattcgccgt
     7921 gccagctaat attcccttgt gagggagact ctaagcctac cagcagccgc gcgagggtgc
     7981 tttttccgca gccgctgcgt cccagcaggg cgacggtttc gccgcttttc agggtcaggg
     8041 aaacgttatt cagcaccgcc tgatgttgat gttttccgct aaatccaccg tgggcatagt
     8101 gatgggaaag gccgcagacg ttaagtaagg tcatgatgcc agctccatac cgtagagggc
     8161 gagatgagcg gaaaccaggc tgcgcgtcac cgtatgtttg ggggcgttaa acagcgtttc
     8221 tacatcgccc tgttcgacaa ttttaccttg tgacatcacc gccacgtcat ccgccagacg
     8281 cgccacaacg cccatatcat gggtgaccag cagcattccc ggcgcttgtt tttgcataat
     8341 gctttccagc agatcgagga tgcgcgcctg tgctaccacg tcgaggtcgg tggtcggttc
     8401 atcggcgatg ataaacggtg attcacacag caccgccatc gcaatcatca tgcgctgcaa
     8461 catgccgccg ctcatctcga acgggtacag cttcagcacg cgcgcggcgt tttccagccc
     8521 caccgcttct atggcagcgg taagcgtagc gtcatcggcg ggtttcccta acgccaggca
     8581 ggtttcccgc gcgtgggtgt gcatggtgtg cagtggatta aaggcgctgc gcgggttctg
     8641 catgatggtg gcaattttga tgccgcgcag ggcgtaaggc gaaaccggtt tgccatcggc
     8701 taaaatttcc cccgccgtct ggcgaacgcc agcgggcaaa atgcccagcg tcgcggcgca
     8761 ggttaatgat ttcccgctgc cgctaccgcc gactaacgcc agcacgcgcc cgcgttgcag
     8821 ggttaacgat acaccgtgta ccagcggctg cgcggcctgt agcgtgatat tacgtagttc
     8881 aatctgttgc ggcattagtg tgcgtgctcc gtcaccagat gaggatccag atgatcgcgc
     8941 agtgcgtcac ccaccaggtt aaaggccatc acgctgataa acagcgccag ccccggccag
     9001 aacatttgca gcggctgggt ccagatatac tggcgcgcgt cgttaatcat cacgccccat
     9061 tcggcggtcg gcgcggtcac accgaggcca aggaaagaca tccccgcgac gtgcagcatc
     9121 atatggccga tatccagcgt tgccagcacc agcagcgaag ggatcaccgc gcctgccaga
     9181 tgatcgacaa acacccgcac atggcccgca ccggaaagcc gtgacgccag cacaaactcg
     9241 cgttggcgta gtgaaatcac caagctgcgc accatgcgtg cataccacgc ccagtgcgac
     9301 agggcgatgg cgataattac gttggtcagc ccggtgccga gcacgccaac cataaagaac
     9361 gacagaatcg aggtcgggaa ggtcataaac atatcggcga cgcgcatggt ggcctgatca
     9421 acgtgcccgc caatcaaccc ggcgctgccg ccaataacca gccctaatgt cagcaccagc
     9481 agcaggcagg ccattaccga accgagcgac acgcgggtcg ctgccatcag ccgcgagaaa
     9541 atatcgcgac ctaagtgatc ggtgcccagc cagtgctgcg catccggcga aagcaggcac
     9601 gacggcaaat caattgcctg tggatcgtac ggctgccacc actggctggt gagcgcaatc
     9661 agcgccagca gggcgataat gatcagcgcc aggcgtaccg accagcggga agagaggaaa
     9721 aagttcacgc gtgcgctcct tcatgacgac gaatgcgcgg gtccagcgcg gcgttgagca
     9781 aatcgacaat caaattacag accacaaaaa ccaccaccat catcagcgta aagcactgaa
     9841 tcaccggata gtcacggtta aaaatcgccg acaccgcata gcgcccgacg ccaggccagg
     9901 caaagatgtt ttcgataatc atcgtcccgc caatcaattc gccaatgtgc atccccacgg
     9961 cggtgatcat cggcagcgag gcattgcgca gaatatggcg gcgttcggtc tgtttgtcat
    10021 tcaggccgcg tagccgcgcc caggtgacgt gacgctgacc ggcgacgtcc agcatactgg
    10081 cgcgcagcag tcgcgcgtta atcgccagcg acataaaggc gatggaaacc gcaggtaaaa
    10141 tgatgtgctg ccagctgccg tagcccatcg cgggtagcca ttgcagatac accgaaaacg
    10201 ccatcactag caaaaatgcc agccagaagt tgggcatcga cacgccaaga aacgcgataa
    10261 agcgcacggc gaaatccggc aaacgatcgc ggtggcgcgc cgcccagata ccgagcggta
    10321 cggaagtgag cagaattaat accagcgccg cacctgcaag ttccagcgtg gcgggcagga
    10381 agttcagcat atcgtccagt accgggcgtt ggctggcgaa tgagatacca aagtcaagat
    10441 gcagcgcctt ccacaaccag gtaccgtact ggacgtacag cggctgatcc agcccgagca
    10501 tggtgcgggt agaggccagc atctccggcg tcggcggcag gttagataaa cgcagataat
    10561 cgagcgccgg gtcgccggta ccgaggcgca gcatcagaaa aatgatcacc gaggcggcga
    10621 gcaccatcgg gatcagcagc agaaagcggc gtaatacgta acgcaacatt aaggtttcac
    10681 cggtttaatc tgttcgaacg gaatttcggt ggcgatcggc gcgtagggga tattacccag
    10741 ctccggtttt gataccacca tcatcgagat gtagctgata ggcagataaa ccgcctcgtc
    10801 atgcagacgg gtcagaatgt cgcgatacag cgcctgacgt tgcgtttcgt catgggtcgc
    10861 cagcacttcg ccgatctctt tatcaatcag cggcttgtcg gctaatcctt gctgtgcctg
    10921 gaagtcagcg tgcgacggta cgcgcattga actcaggaag gcgtgtggat cgtatggcgc
    10981 gccccaggtg cggtggaaaa tcatgccaaa acgaccgtcg cgctgacgag catagatact
    11041 gctctcttct tcgccaatca gcgagacatc tgcgccaatc tggcgcatat cagcctgaat
    11101 gatttccgcc atcgatttgc ttaacgcatc ggtgccgatg aacgacagtt caatgcgcag
    11161 cggctgacca tttttctcgc ggatgtcttt gcccgcaggc agcgtccaac cggctttttc
    11221 cagcaactct ttcgctttct gcggatcgta ctggcgcggt ttcaggccga gattagcgta
    11281 aggcacagaa ggggcaaaca gggtgtcggc gacctgctgg gtgccatata acgcgttatc
    11341 aatcagcgat tttttgttta ccgcgtaatt aagagcttca cgtactgcca gctcgttggt
    11401 gggggctttg gcggtattga gcgccagcat cacggtttcg atcggctgtg acagttgggt
    11461 gtggtaagcc ggattctggc taaagcgggc gaaggtatcg agcggtaata acccttcgtt
    11521 tccgtacagc aggtcgatat cgccagtttc aaacgccacc gcgcgggtag tcgggtccgg
    11581 gatgacgtta aaggtgatct ttttaatcgc tggcttttcg ccccagtagt tttcgttacg
    11641 gacgaagaca tcgtactgat ttagtttcga ttcctgcaaa atccacggtc cggtgccaat
    11701 cggcgcttta attccgttca tggtttcatg gtttttaaac tgcgagggag cgataaaacg
    11761 gaaaggacgg ggcagggcca gttcttgcag gaaaggatag taggcgcttt tcagggtaat
    11821 ttgcagctct gttttattga gtgctttaac atcaacaatc tggtttgcca gctccagcca
    11881 ggcgtgacgt tgacggttat cgagcactgc gcggaagttt tctgccgccg cctcggcatc
    11941 gaacggttca ccgttggaga atttcacgtc atcacgcagg gtgaaggtcc aggttttacc
    12001 atcttctgaa tgagtccagc tttttgccag ccacgggatc accgaaccgt ctgcctgata
    12061 tttcaccaat ggttcataaa ccatgctctg ggcgaacatc tggttaggcg tgtaaaggtg
    12121 cgggtttagt ggcccgacat tcaccggcca ggcggtggtg atttcatctg gtgcagcggc
    12181 atggacgata aaagacgcac aagccagcag cgcaaataga gtgcggcgga gtgtggagag
    12241 catggtaacc ccaatggatt aaaatagatg gcggaaataa gtatgacgat tttaagtatt
    12301 cgtcatactg attacctgtt ctggatcaat agatgggcag tcgggtgggc ggatcagtta
    12361 actgaatcga tccattgcac actgtcggcg gtgagcgtaa agggggtggg ggtgcagatc
    12421 gcaaggctta aattttctag ctgacaatgg ctgactgaca gcgaggagtg ataggtgcta
    12481 tctacgctga cgatttgcca ggcgctgccg ccacgctgtt taacgatggc ttctttgcgc
    12541 gtccagatgc gccagaacat ttccagttgc tgatcaggat gcacggcgtc catttcagcg
    12601 tgttccccga ggctgaacac ggcgttcgcc agccagcgcc agttgacgcg cgggcgaatc
    12661 acttcgatat cgcagccgac ttcgccttca tcgctcaaca gcagagcgat atcgtcaccg
    12721 ctatggctta agttgaacca tagcggcgtt tccggcgcga atgcaggttt gccttgttcg
    12781 ccatagatga tctccggtag cggggaaagc gtgtgcgaaa gcaatgtacg ccccgccagc
    12841 cagcgttcgc gtcgtggacc ttgcggtgct tgctcgcgta aacccggtgg cagtggagct
    12901 gcgcttaagg tcgaaacttt ccccagaact atccgataca tatcagggcc aacgtttaat
    12961 ggaaaatgaa agtgcgtatc gtatcacttg tcgcctcatc ccggtaaccg acttttcggt
    13021 ctgcccggcc ccagtaaaat cgccagtttg ctgccacctt tcgtcgtttc catccagatt
    13081 ttacacacgc tggttaatgg caccgaaagt agcatcccta ccgggccgag cagccatccc
    13141 cagattaaca atgaaagaaa taccaccatg gtggacatcc ccaggcgatg gcccatcatc
    13201 cgtggttcta aaatattgcc gatgaccata tggaccacta aaaacaatgc gccgaccaga
    13261 atacattcgt aaacaccatt aaacagcagc acctgaatca ttggcggtac ggcggaaatt
    13321 accgcgccga tattgggcac gtagttgagc aaaaacgcca gtactgccca catcagcgca
    13381 aactgtacgc ccattagcgc cagccccagc cagacgatga cacctgtcca taaactgagt
    13441 agcgtcttta acgccagata atgcgaaacg cctttaagtg cgcggtgtaa tcctgcgatg
    13501 tgaatctgtg ggttattcag cgcaaaacgc attttgtaag ggacgtggcg cacttcaaac
    13561 agcataaaaa ctacggtcat caccagcaaa agcacgctcg ccattgcccc ggaaagcccg
    13621 gtcattagcg ctgtggtgaa ggtaaccact ttttccgagt ccatccgctg cagcattcgc
    13681 tccggcgaca tatgcaaatt aagaaaaggc aacatctcct gcaatttaaa aagtttgcgc
    13741 gtcagctcct tattaaactt cggcagcata gagataaatt cgttaaacga tgccgccagt
    13801 acgccgacca gcgcggttag tgcgatcagc atcaccacca ctacaatcgt aatggcaacg
    13861 gggcgttgta ctccccgacg aataaaccag gtaacgagcg ggttgaggac gatggcaaaa
    13921 aacagcgcca gtaacagctg cacaatgata tctgccgctg cgtgaatgcc cgcgaggatc
    13981 actaccagcg aggccaactt gagcagaatg tgcatgcccg ttttatcggg ttgaggggtt
    14041 tccattgggg cttccttgtg actttttgtg ttaagtgtag cgcgggagtc acgccagcaa
    14101 tattctgatt cttactgctg attttcacgt cagcacatgg taaaaatgaa acactgttgt
    14161 aaaaatgtgg tgatcctcat gcccgaaccc gtagccgaac ccgcgctaaa cggattgcgc
    14221 ctgaatttgc gcattgtctc cattgtcatg tttaacttcg ccagctacct caccatcggg
    14281 ttgccgctcg ctgtattacc gggctatgtc catgatgtga tgggctttag cgccttctgg
    14341 gcaggattgg ttatcagcct gcaatatttc gccaccttgc tgagccgccc tcatgccgga
    14401 cgttacgccg atttgctggg acccaaaaag attgtcgtct tcggtttatg cggctgcttt
    14461 ttgagcggtc tggggtatct gacggcagga ttaaccgcca gtctgcctgt catcagcctg
    14521 ttattacttt gcctggggcg cgtcatcctt gggattgggc aaagttttgc cggaacggga
    14581 tcgaccctat ggggcgttgg agtggttggc tcgctgcata tcgggcgggt gatttcgtgg
    14641 aacggcattg tcacttacgg ggcgatggcg atgggtgcgc cgttaggcgt cgtgttttat
    14701 cactggggcg gcttgcaggc gttagcgtta atcattatgg gcgtggcgct ggtggccatt
    14761 ttgttggcga tcccgcgtcc gacggtaaaa gccagtaaag gcaaaccgct gccgtttcgc
    14821 gcggtgcttg ggcgcgtctg gctgtacggt atggcgctgg cactggcttc cgccggattt
    14881 ggcgtcatcg ccacctttat cacgctgttt tatgacgcta aaggttggga cggtgcggct
    14941 ttcgcgctga cgctgtttag ctgtgcgttt gtcggtacgc gtttgttatt ccctaacggc
    15001 attaaccgta tcggtggctt aaacgtagcg atgatttgct ttagcgttga gataatcggc
    15061 ctgctactgg ttggcgtggc gactatgccg tggatggcga aaatcggcgt cttactggcg
    15121 ggggccgggt tttcgctggt gttcccggca ttgggtgtag tggcggtaaa agcggttccg
    15181 cagcaaaatc agggggcggc gctggcaact tacaccgtat ttatggattt atcgcttggc
    15241 gtgactggac cactggctgg gctggtgatg agctgggcgg gcgtaccggt gatttatctg
    15301 gcggcggcgg gactggtcgc aatcgcgtta ttactgacgt ggcgattaaa aaaacggcct
    15361 ccggaacacg tccctgaggc cgcctcatca tcttaaaact tactgaataa ccagcgtatt
    15421 aatgatgttt tctgcggtgg tttgcgcttt ttgctgatcg tcagctggca gcgtaatttg
    15481 catggtcagc agttgattac ccacgttacc cagaataacg gaagagtacg ccgtctggcc
    15541 tttcgcggag ataatactgt ctaactgctg cattttgtga cctttcagct caatggcttt
    15601 attggttacc acttgcagct gcggatcgcg gctacgttgc tgatcttcca gacgcttcgc
    15661 caacaccgcc agatcttctt tcggatcatc gcccatgatg acgatgactg ctttctgccc
    15721 ggtggcgtcg gaccagacat gcatgttatt ggcctgcgtt cccagcttac cgctctggtc
    15781 ggtcatatcc gctggcagcg agaaacttaa cttgccatca agcaggttga cgggattccc
    15841 ggtagcgtta ctttccgcaa ccgaaccctg cgccgtagcg ttagtgtctt tatcatcaca
    15901 ggccgcaagc cccataacca gcaggccaat tccgacatat ttaaccagat tgcgcattga
    15961 cttcttcctt tcgataaacg gccataacgg ctcattcatc catcttatca caactctgat
    16021 aacgaacctt taactcgcct gcaaagcgtt gatttcggat ttatctgcca gtcttttcaa
    16081 cagcatattg agtaatacgc catacattgg caggaagaaa acgatactga ttaacacttt
    16141 gaaacagtaa tcgaccagcg cgatttccat ccagtgttca gccataaagg catccgggct
    16201 acgccagaag gcaatgaaga aaaaggccag cgtgtcgctg acgttaccga acagtgtgga
    16261 cgctgtcggt gccagccacc agcggcgact ctgacgcagg cggttaaaaa cgtgcacgtc
    16321 gaggatttgc cccagcgcgt aggccatgaa actggcggtg gcgatacggg cgacaaacag
    16381 gttgaagtgg gcgagtgcgc cgaatccctg ccaggaaccc atatagaata gcgacgagat
    16441 gacgtaggag attaataacg cagggatcat taccgcgaag ataatgcgtc gggccagcgg
    16501 tgcgccaaaa atacgcacgg tcaggtcggt agcaagaaaa ataaacggaa agctaaacgc
    16561 gccccaggtg gtatggaaac caaaaatgga gacgggaagc tgaaccagat agttactgga
    16621 ggtgatcacc agaagatgaa ataacgataa ccagaacaac gccttatagc gttgagtttg
    16681 cgagaaaacg ttcatattgt acctttttga ttaaccattg gggtgaggga acccaatacg
    16741 tacgacacgt ctgttacctt aacataataa cgtgcttacc cttttttcga gccgccgcat
    16801 gatactgctt tacgttgaca atgcaatggt taattttcac gcaatcgtta acctggtttg
    16861 cttacggact cacagggcgt aaactagcgc cgttttttta agtgatgaga agaaaatgac
    16921 cgatctcttt tccagccctg accacacact cgacgcgctt ggcctgcgct gcccggaacc
    16981 ggtgatgatg gtgcgcaaaa ccgtgcgcaa tatgcagcct ggcgaaacgt tgctgattat
    17041 cgccgacgat ccggccacta cccgcgatat tcctgggttt tgtaccttta tggaacacga
    17101 actggttgcg aaagagacgg atggactgcc ttatcgttat ttgattcgta aaggcggttg
    17161 ataggggctg attggcttcg atgccgcctt ttcctcaccc cctaaccctc tccccagagg
    17221 ggcgagggga ccgatcgcgc tcaatgttgc gatcggtttg ccttatctcc tgcgcaacaa
    17281 ccttaacgca ttcgccgtca ccagcaccgt cgcacccgta tctgccagca ctgccagcca
    17341 caacccggtc atccctaaaa gcgtggtgac gaggaagatc cctttcagtc ccagcgcaat
    17401 ggtgatgttc tggcggatat tggcgtgagt ggcgcgtgcc agttcaatca tttgcaccag
    17461 gccgcgcagg tggttatggg ttaatgctgc gtcggcggtt tccagcgcca cgtctgtgcc
    17521 gctacccata gcaatcccga tggcggcagc tttcatcgct ggcgcgtcgt taataccatc
    17581 accgaccatc gccaggggcg catgttgatt cagcttggtc accgctttga ctttatcttc
    17641 cggcaatagg cccgctttaa actccagccc cagctccccg gcaattgccg ccgctgcgcg
    17701 tggattatcg ccggtgagga tcactccttt aacccccagc gcgttcagtt cactgatggc
    17761 agttgtagcg tcggcgcgca gggtatcctg caatgcaatg atacccagca cgtcatcatt
    17821 acgtactacc agcacaaccg tttgcccggc gctttccagt tcattaatca aaccagcaaa
    17881 tgcatcagcg ggatgtttcc cggcagcgca tatcaacacg cgctcaccat taacctgcgc
    17941 ttcaatgcca gacccgacca gtgcccgctg tgattcggcg gtgggaatgg cgagttcagc
    18001 aacctgtgct tcgcgtacga tggcttgcgc cagtggatgc gtcgcgcctt gctcgaccgc
    18061 cgccgccagt gtcagcagtt cagattcact aatacccgtt gccggatgaa tcgcggtaac
    18121 gcgcggttta ccgacggtca gcgtaccggt tttatcaaac gccacttggg taacacgacc
    18181 tagttgttcc agcgccgcac cgcctttaat taacgcccca cgacgcgctg ctgctgccag
    18241 cccggaggtt atcgccgcag gcgttgagat aactaacgca cacgggcaac caatcagcag
    18301 cagcgtcaga cctttataaa tccactcctg ccagctggcg gcaaacagca gcggcggcac
    18361 tagcgttacc agcagggcga cggccataat cgctggcgta taaatacggc tgaaacggtc
    18421 gataaaccgc tcaatggggg cgcggcgctc ttcggcttct tcgatcagtt tcagaatccg
    18481 gtcaatggcg ctggcacccg gttctgacag cacttccagc gttaccagac ggtctacgct
    18541 ggtggctcct gcaggaactt tatcgcccgt cgcgcgctcc accggaatgg attctccggt
    18601 tagggcgctt tcatcaaaac tggcaaacgg tgagagcagt ttaccgtcgg caggcaaacg
    18661 cccacctgcg gcgacttcaa tcacatcgcc aggccgcagg ctgttaatcg ccacctcttc
    18721 ccgctcaccg ttacgcaggc gcgtggcggt ttctggtttc agcgccatta acgcgctaac
    18781 gccctgacgc gcgcggctgg cggcccagcc ttccagtcgt tcaccaatca aaaacagcag
    18841 caacaccatc gcagcttcag ccgttgcgcc aataaacagt gcaccaatag cggctacgct
    18901 cattaaggtt tcaatggcga agtagctgcc ggatttgatc agccgtaatg cctgacgagc
    18961 aatcgggtac agcccaacta gcgtggtcgc gataaacgcc agttgcccga acggatgatt
    19021 gaactgctcc agaccccagc tgattgccat catcacgata agcgtaatca gcggcagatt
    19081 ctctttcagg cgcgatgctt gtggctcgtc ggcggcctgc tcatcgcgca gggaatagcc
    19141 cgctttttgc accgcagatt caacctgggc acggatgtca ttgtcggcat caaccaccag
    19201 tttttcggtg gcgaacaaca cctgcacctg attcacgcct gcaagctggc gcacggcatt
    19261 ttctaccttg cgcgcacagg cggcgcagtc catgccgctg actttccagc tatagcgggt
    19321 gccggagacg ttttcagaga gcgttggcga gctggagcat gcgccgtcgc agcaacagtc
    19381 gttggcgttc tgtaccgtgg ttagcggttt gaacgcagca aattgagggg ctttcttgcc
    19441 gtgattgtca ggtgtcgaca tggcatcctc cggttaagtt ttttctcatt aaccgaagga
    19501 tacactctgg agtcgactcc agagtcaagt tttatcagag atacagtgag cggacgatga
    19561 ggaagtgacc ggcaaagtag caggccgcag caatcgcgtt atccgcacgg aagcgtcggc
    19621 gatagtggct ccccagccag acaaagttac tgataaacag caacgaagcg ccaacaaacg
    19681 cagagagcgc cggagcggtc ggacggaaga accacagttc acctgccagc cacaccatca
    19741 ccagcgtcat gccgataaac gtgcagatag gccaacggta ctcttccagc cgcgtccaga
    19801 taatcgccag tagcagcgca cccagaacca gcaacaccag tggcagaggc cagaagaaag
    19861 agagcgtcat ctgactggcg aaatagatgg tgtacagcag gtgcgagagg aaaaacgcgc
    19921 cgatggcgta catcagacgt tgacgtggca acagggttag cgcatcgccc agcagtgagg
    19981 cgcacagtcc cgccagcacc agatagctaa tggcgtcgaa catcggtgct tgccaggcca
    20041 gtaacagcag gagaagaagg gttaacggtt taaacaccca gcgttgccag gttggcccac
    20101 gatacgatgc atccacagat agccatgcgg aaagacagac agcgataaac gaccaaagca
    20161 tcttagttcc ttgaattcgt tattttttcg taagcagaat gcctacggtc ttgcttccag
    20221 tttagtggcg agggtggacg gatgacaaca ccataactgg cagggtatgc ttaattccgc
    20281 attttccgat gagggataaa gatgagtaaa ccacctcttt tctttattgt tatcattggc
    20341 ttaattgtcg tcgccgcatc gtttcgtttt atgcaacagc gacgggaaaa agctgataat
    20401 gatatggctc cgctccagca aaagctggtg gtggtgagca acaagcggga aaaaccgatt
    20461 aacgatcgcc gttcgcgcca gcaggaagtg actccggcag gcaccagtat gcgctatgag
    20521 gcaagcttca aaccgcaaag cggaggaatg gagcagacgt ttcgcctcga cgcccagcag
    20581 taccacgccc tgacagtggg cgataaaggt acgctgagct ataaaggaac gcgctttgtt
    20641 agctttgttg gcgaacaata aggttatttt ttaactttaa atttcttctg ccagaccagc
    20701 agttcaaaca cgccgaaaag gaaaatccgc accttttcgg cggtggtcat ctgcgggcca
    20761 tctttcggta aagtggattt caatagcgcc agctgcatac catgcatcag cacggtaaaa
    20821 atcagcgcca cgttaacgaa aatatttagc gggcgcggga agggatgcac cagattgagg
    20881 attaaaaatc cccaaacgca gagcattaac aaacgaccaa tattaatcag catcactttc
    20941 tccttgtgct tcgtgttgat acagccgata agccacctga cccgccactt tttcccgatg
    21001 taatgaccag tttgccggaa cagtgggcag accgttttcg acttcgcttt cgacataaat
    21061 cagggcttcg tcagccagcc agccgttatc ttccagtaaa tttatcgtct cttctaacaa
    21121 gccacggcgg aacggtggat cgacaaacac gatattatgc ggtgtgcctt tttgcgccag
    21181 gaatgacatt gcgttgctgt tcaccacgcg tgcattgcct gcttttagtg tcgccagatt
    21241 cttaattaac tgctgagaaa ccacgcgatc catctcaatc aacgttgccc ccgcagcgta
    21301 gcgcgataac gcttccagcc ccagcgcgcc gctcccggcg aagcaatcca gacattgggc
    21361 gtcaacaatg accggagcca gccagttaaa caacgtttcg cgtacgcggt cggtggtggg
    21421 gcgcagacct gggctatcag gaaccgggag tttacggcct cgccactgcc cgccaataat
    21481 gcggatttgg ccgctgccgg aatgattcgg ttttttcatg ataaattgct caatccgcca
    21541 gataacacat acttgcaggc ggtgatgtga attaagttaa gtgatagact atttcatcat
    21601 ttttttagct gctatgtaca tagcgttaac gctgtgccat gaagcaacag cgaggagtgt
    21661 agtcgcaaat ggcgaaagaa aaaagacgtg gctttttttc ctggctgggc tttggtcaaa
    21721 aagagcagac cccggaaaaa gagacagaag ttcagaatga acaaccggtt gtagaagaaa
    21781 tcgttcaggc gcaagagcct gcgaaggctt ctgaacaagc cgttgaagag cagccgcagg
    21841 cgcatactga agccgaggcg gaaacttttg ctgccgacgt tgtggaagtc actgaacagg
    21901 ttgttgaaag tgaaaaagcg cagcctgaag cggaagttgt tgcacagccg gaaccggtcg
    21961 tagaagaaac gccggagcca gtggctatcg aacgtgaaga gctgccgttg ccggaagacg
    22021 tcaacgccga agaggtttcg tcagaagagt ggcaggctga agcggaaacc gtagagattg
    22081 tcgaagcggc agaagaagaa gcggctaaag aagaaatcac cgacgaagag ctggaagcac
    22141 aggcgctggc tgccgaagcg gcagaagagg cggtgatggt agttcctccg gcagaagaag
    22201 agcagccggt tgcagaaatt gcccaggagc aagaaaaacc gaccaaagaa ggcttcttcg
    22261 cgcgcctgaa acgcagcctg ttaaaaacca aagaaaatct cggttccgga tttatcagcc
    22321 tgttccgcgg taaaaaaatc gacgatgatc tgtttgaaga gctggaagaa cagctgttga
    22381 tcgccgatgt gggcgtggaa accacacgta aaattatcac caatctgacg gaaggcgcat
    22441 cccgcaagca gcttcgtgat gccgaggcgc tctatggcct gctgaaagaa gagatgggcg
    22501 agattctggc gaaagtcgat gagccgctga atgttgaaga taaaacgccg ttcgtgatcc
    22561 tgatggtggg cgtcaacggt gtgggtaaaa ccacgacgat tggtaagctg gcgcgtcagt
    22621 ttgagcagca gggtaaatcg gtgatgctgg cggcgggcga tactttccgt gcagctgcgg
    22681 ttgaacagct tcaggtctgg ggtcagcgca acaatattcc ggtgattgcc cagcataccg
    22741 gtgcggattc cgcctctgtt atcttcgacg ccattcaggc agctaaagcg cgtaatatcg
    22801 acgtcctgat tgccgatacc gccggacgtc tgcagaacaa atcgcatctg atggaagagt
    22861 tgaagaaaat cgtccgcgtg atgaagaaac tcgacgttga agcgccgcat gaagttatgc
    22921 tgactattga tgccagcacc gggcagaacg cggtaagcca ggccaaactg ttccacgaag
    22981 ctgttggctt aaccggcatc acgctaacga aactggacgg cacggcgaaa ggcggggtaa
    23041 ttttctcggt ggctgaccag tttggtatcc ctatccgcta cattggtgtc ggcgaacgta
    23101 ttgaggattt gcgtccgttt aaggcggacg actttataga ggcacttttt gcccgagagg
    23161 attaacaatg attcgctttg aacatgtcag caaggcttat ctcggtggga gacaggcgct
    23221 gcagggcgtt acgttccata tgcagccggg tgagatggcg tttctgaccg gtcattccgg
    23281 cgcagggaaa agtaccctcc tgaagctgat ctgtgggatt gagcggccca gcgccgggaa
    23341 aatctggttt agcggccatg acatcacgcg tctgaaaaac cgtgaagttc cgtttctgcg
    23401 ccgccagatt ggcatgattt tccaggatca ccatctactg atggaccgta ctgtctacga
    23461 taacgtggcg atcccgctga ttatcgctgg tgccagcggt gacgatattc gtcgccgggt
    23521 gtcggcggca ctggataaag tcgggctact ggacaaagcg aagaacttcc ctattcagct
    23581 ttccggcggt gaacaacagc gtgttggcat tgcccgcgcg gtggtgaata agcccgcggt
    23641 actgctggcg gacgaaccga ccggtaacct ggacgacgcg ttgtcggaag ggattctacg
    23701 tctgtttgaa gagtttaacc gcgttggggt aaccgtattg atggcaacgc acgacattaa
    23761 tctgatttca cggcgttcct atcgcatgct caccctgagc gatggtcact tgcatggagg
    23821 cgtgggccat gaataagcgc gatgcaatca atcatattcg gcagtttggt gggcgtctgg
    23881 atcgcttccg taaatcggtc ggcggctcag gcgacggcgg tcgtaacgca ccgaaacgcg
    23941 cgaaatcctc gccaaaaccg gtaaatcgca aaaccaacgt tttcaacgaa caggtgcgct
    24001 atgccttcca cggcgcattg caggatctga aaagcaaacc gttcgccacg tttttaacgg
    24061 tgatggttat cgccatctcc ctgacgctgc ccagcgtctg ttatatggtg tacaaaaacg
    24121 ttaatcaggc ggcgacgcag tattatccgt caccgcaaat cactgtttac ttgcaaaaaa
    24181 cgctggacga tgacgccgct gcgggcgtgg tggcacagtt gcaggccgag caaggcgtgg
    24241 agaaagtgaa ctatctttct cgtgaagacg cactgggcga gttccgtaac tggtctggtt
    24301 ttggtggtgc gctggatatg ctggaagaaa acccgcttcc ggcagtggcg gtggtgatcc
    24361 cgaaactcga ttttcagggg acggaatcac tcaatacgct gcgtgatcgt atcacgcaga
    24421 ttaacggtat tgacgaagtg cggatggatg acagctggtt tgcccgtctg gcggcgttga
    24481 ccgggctggt tgggcgcgtt tcggcgatga tcggcgtgtt gatggtggcg gcagtgttcc
    24541 tcgtcatcgg caacagcgta cgtctgagca tctttgctcg ccgtgactcc atcaacgtgc
    24601 agaaactgat tggtgcgacg gatggtttca tccttcgccc gttcctgtac ggcggtgcgc
    24661 tgctgggatt ttctggcgca ttgttgtcat taattttgtc agaaattctg gtactgcgtt
    24721 tgtcgtcagc ggtggcggaa gtggcacagg ttttcggaac gaagtttgat atcaatggtt
    24781 tatcgttcga tgaatgcctg ctattgctgc tggtatgctc gatgattggc tgggtggcag
    24841 cgtggcttgc cacggtacaa catttacgcc actttacgcc tgaataataa aagcgtgtta
    24901 tactctttcc ctgcaatggg ttccgtagca gggaaagaga ccccgttgtc tcttcccggt
    24961 atttcatctc tatgtcacat tttgtgcgta atttattcac aagcttgcat tgaacttgtg
    25021 gataaaatca cggtctgata aaacagtgaa tgataacctc gttgctctta agctttggca
    25081 cagttgttgc taccactgaa gcgccagaag atatcgattg agaggatttg aatgactgac
    25141 aaaatgcaaa gtttagcttt agccccagtt ggcaacctgg attcctacat ccgggcagct
    25201 aacgcgtggc cgatgttgtc ggctgacgag gagcgggcgc tggctgaaaa gctgcattac
    25261 catggcgatc tggaagcagc taaaacgctg atcctgtctc acctgcggtt tgttgttcat
    25321 attgctcgta attatgcggg ctatggcctg ccacaggcgg atttgattca ggaaggtaac
    25381 atcggcctga tgaaagcagt gcgccgtttt aacccggaag tgggtgtgcg cctggtctcc
    25441 ttcgccgtcc actggatcaa agcagagatc cacgaatacg ttctgcgtaa ctggcgtatc
    25501 gtcaaagttg cgaccaccaa agcgcagcgc aaactgttct tcaacctgcg taaaaccaag
    25561 cagcgtctgg gctggtttaa ccaggatgaa gtcgaaatgg tggcccgtga actgggcgta
    25621 accagcaaag acgtacgtga gatggaatca cgtatggcgg cacaggacat gacctttgac
    25681 ctgtcttccg acgacgattc cgacagccag ccgatggcac cggtgctcta tctgcaggat
    25741 aaatcatcta actttgccga cggcatcgaa gatgataact gggaagagca ggcggcaaac
    25801 cgtctgaccg acgcgatgca tggtctggac gaacgcagcc aggacatcat ccgtgcgcgc
    25861 tggctggacg aagacaacaa gtccacgttg caggaactgg ctgaccgtta cggtgtttcc
    25921 gctgaacgtg tgcgccaact ggaaaagaac gcgatgaaaa aacttcgcgc cgctattgaa
    25981 gcgtaatttc cgctattaag cagagaaccc tggatgagag tccggggttt ttgttttttg
    26041 ggcctctaca ataatcaatt ccccctccgg caaaacgcca atccccacgc agattgttaa
    26101 taaactgtca aaatagctat tccaatatca taaaaatcgg gtatgtttta gcagagtatg
    26161 ctgctaaagc acgggtagtc atgcataaaa cgaaataaag tgctgaacaa caacatcaca
    26221 acacacgtaa taaccagaag aatggggatt ctcaggatga acataaaggg taaagcgtta
    26281 ctggcaggat gtatcgcgct ggcattcagc aatatggctc tggcagaaga tattaaagtc
    26341 gcggtcgtgg gtgcaatgtc cggtccggtt gcgcagtacg gtgaccagga gtttaccggc
    26401 gcagagcagg cggttgcgga tatcaacgct aaaggcggca ttaaaggcaa caaactgcaa
    26461 atcgtaaaat atgacgatgc ctgtgacccg aaacaggcgg ttgcggtggc gaacaaagtc
    26521 gttaacgacg gcattaaata tgtgattggt cacctctgtt cttcatcaac gcagcctgcg
    26581 tctgacatct acgaagacga aggcatttta atgatcaccc cagcggcaac cgcgccggag
    26641 ctgaccgccc gtggctatca gctgatcctg cgcaccaccg gcctggactc cgaccagggg
    26701 ccgacggcgg cgaaatatat tcttgagaaa gtgaaaccgc agcgtattgc tatcgttcac
    26761 gacaaacagc aatacggcga aggtctggca cgagcggtgc aggacggcct gaagaaaggc
    26821 aatgctaacg tggtgttctt tgatggtatc accgccgggg aaaaagattt ctcaacgctg
    26881 gtggcgcgtc tgaaaaaaga gaatatcgac ttcgtttact acggcggtta tcacccggaa
    26941 atggggcaaa tcctgcgtca ggcacgcgcg gcagggctga aaactcagtt tatggggccg
    27001 gaaggtgtgg ctaacgtttc gctgtctaac attgcgggcg aatcagcgga agggctgctg
    27061 gtgaccaagc cgaagaacta cgatcaggtt ccggcgaaca aacccattgt tgatgcgatc
    27121 aaagcgaaaa aacaggaccc aagcggcgca ttcgtctgga ccacctacgc cgcgctgcaa
    27181 tctttgcagg cgggcctgaa tcagtctgac gatccggctg aaatcgccaa atacctaaaa
    27241 gcgaactccg tggataccgt gatggggcca ctgacctggg atgagaaagg cgatctaaag
    27301 ggctttgagt tcggcgtatt tgactggcac gccaacggca cggcgaccga tgcgaagtaa
    27361 ttgttaatcg gcaactttgg gttgccgcca aattgctaat atcgaatacg ttgcttcatg
    27421 ccggatgcgg cgtaaacgcc ttatccggcc tacaagaacc aaagaaatca gtaaattgca
    27481 gcacacatta taggcctgat aagcgtagcg catcaggcaa tttctcgttg gtcatcactt
    27541 ttgaaatcaa cttgacgatt aacgcttctc ccagccgccc tgctgtgccg taaatcccag
    27601 tgcctgcata aacgccgtca tcacaccgcg atcttccacg cctgcatccg ccatccacca
    27661 gcatgaaacg ccaggattgt tacgcaaaac ctcttccagc agatattgcc ccacaccgcg
    27721 acggcgggtg acttcccgca cgcgtagaga atccagtgct ccctcggtgc cgcttaaggt
    27781 tacccgcacg gccgcaagca gacgctcgtt aaaacgcgcg gcgtagatac ggtggttatc
    27841 gtcaacctgt aaagaggaag gggaatactc cggccagatc ttttgcaggt caatccggtc
    27901 ctggtcgcta aagttttcta atcgaatgat ggtcagcttc atgggtaacc cgtgtaaatc
    27961 acaaaagtgg aaccagtgta gcgaaataat ttaatcggag gctttctctt ttttatttct
    28021 tttggcaggt gattaatttt ttaacagcaa taattacaaa gttaaaac
//
LOCUS       DAZOYF010000038        27845 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZOYF010000038 DAZOYF010000000
VERSION     DAZOYF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27845
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.038"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..188
                     /locus_tag="ACHUOF_001552"
     CDS             <1..188
                     /locus_tag="ACHUOF_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HFR0610786.1"
                     /translation="TTRKDDQEETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVD
                     GTKQVAEVRADLEKILG"
     gene            320..1282
                     /gene="hemH"
                     /locus_tag="ACHUOF_001553"
     CDS             320..1282
                     /gene="hemH"
                     /locus_tag="ACHUOF_001553"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR0610787.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(1279..2238)
                     /gene="aes"
                     /locus_tag="ACHUOF_001554"
                     /pseudo
     CDS             complement(1279..2238)
                     /gene="aes"
                     /locus_tag="ACHUOF_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            2390..3694
                     /gene="gsk"
                     /locus_tag="ACHUOF_001555"
     CDS             2390..3694
                     /gene="gsk"
                     /locus_tag="ACHUOF_001555"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR0610788.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(3827..5503)
                     /gene="ybaL"
                     /locus_tag="ACHUOF_001556"
     CDS             complement(3827..5503)
                     /gene="ybaL"
                     /locus_tag="ACHUOF_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR0610789.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(5741..6961)
                     /gene="fsr"
                     /locus_tag="ACHUOF_001557"
     CDS             complement(5741..6961)
                     /gene="fsr"
                     /locus_tag="ACHUOF_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR0610790.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            7179..8831
                     /gene="ushA"
                     /locus_tag="ACHUOF_001558"
     CDS             7179..8831
                     /gene="ushA"
                     /locus_tag="ACHUOF_001558"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR0610791.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(8868..9347)
                     /gene="ybaK"
                     /locus_tag="ACHUOF_001559"
     CDS             complement(8868..9347)
                     /gene="ybaK"
                     /locus_tag="ACHUOF_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR0610792.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(9551..10345)
                     /locus_tag="ACHUOF_001560"
     CDS             complement(9551..10345)
                     /locus_tag="ACHUOF_001560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR0610793.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            10483..10824
                     /locus_tag="ACHUOF_001561"
     CDS             10483..10824
                     /locus_tag="ACHUOF_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0610794.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(11039..13543)
                     /gene="copA"
                     /locus_tag="ACHUOF_001562"
     CDS             complement(11039..13543)
                     /gene="copA"
                     /locus_tag="ACHUOF_001562"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR0610795.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            13805..14737
                     /gene="glsA"
                     /locus_tag="ACHUOF_001563"
     CDS             13805..14737
                     /gene="glsA"
                     /locus_tag="ACHUOF_001563"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR0610796.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            14740..16032
                     /locus_tag="ACHUOF_001564"
     CDS             14740..16032
                     /locus_tag="ACHUOF_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR0610797.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            16063..16160
                     /locus_tag="ACHUOF_001565"
                     /pseudo
     CDS             16063..16160
                     /locus_tag="ACHUOF_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16157..16564
                     /gene="cueR"
                     /locus_tag="ACHUOF_001566"
     CDS             16157..16564
                     /gene="cueR"
                     /locus_tag="ACHUOF_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR0610798.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(16565..17023)
                     /locus_tag="ACHUOF_001567"
     CDS             complement(16565..17023)
                     /locus_tag="ACHUOF_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR0610799.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(17020..17937)
                     /locus_tag="ACHUOF_001568"
     CDS             complement(17020..17937)
                     /locus_tag="ACHUOF_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR0610800.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            18083..18760
                     /gene="fetA"
                     /locus_tag="ACHUOF_001569"
     CDS             18083..18760
                     /gene="fetA"
                     /locus_tag="ACHUOF_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR0610801.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            18747..19526
                     /gene="fetB"
                     /locus_tag="ACHUOF_001570"
     CDS             18747..19526
                     /gene="fetB"
                     /locus_tag="ACHUOF_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR0610802.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(19589..20443)
                     /gene="cnoX"
                     /locus_tag="ACHUOF_001571"
     CDS             complement(19589..20443)
                     /gene="cnoX"
                     /locus_tag="ACHUOF_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR0610803.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(20504..21313)
                     /gene="ybbO"
                     /locus_tag="ACHUOF_001572"
     CDS             complement(20504..21313)
                     /gene="ybbO"
                     /locus_tag="ACHUOF_001572"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0610804.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(21303..21925)
                     /gene="tesA"
                     /locus_tag="ACHUOF_001573"
                     /pseudo
     CDS             complement(21303..21925)
                     /gene="tesA"
                     /locus_tag="ACHUOF_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            21896..22582
                     /gene="ybbA"
                     /locus_tag="ACHUOF_001574"
     CDS             21896..22582
                     /gene="ybbA"
                     /locus_tag="ACHUOF_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR0610805.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQLFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            22579..24994
                     /gene="ybbP"
                     /locus_tag="ACHUOF_001575"
                     /pseudo
     CDS             22579..24994
                     /gene="ybbP"
                     /locus_tag="ACHUOF_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
     gene            complement(25212..25367)
                     /gene="dinQ"
                     /locus_tag="ACHUOF_001576"
     CDS             complement(25212..25367)
                     /gene="dinQ"
                     /locus_tag="ACHUOF_001576"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR0610806.1"
                     /translation="MKKIIVIGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALLE
                     LIRQFIE"
     gene            complement(25707..26801)
                     /gene="mnmH"
                     /locus_tag="ACHUOF_001577"
     CDS             complement(25707..26801)
                     /gene="mnmH"
                     /locus_tag="ACHUOF_001577"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR0610807.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(26870..27796)
                     /gene="allS"
                     /locus_tag="ACHUOF_001578"
     CDS             complement(26870..27796)
                     /gene="allS"
                     /locus_tag="ACHUOF_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFR0610808.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
BASE COUNT         6606 a         7323 c         7381 g         6535 t
ORIGIN      
        1 tgactacccg taaagacgat caggaagaaa ccgtgcgtaa acgtctggtt gaataccatc
       61 agatgactgc accgctgatc ggctactact ccaaagaagc ggaagcgggt aacaccaaat
      121 acgcgaaagt tgacggtacc aagcaggttg ctgaagttcg cgctgatctg gaaaaaatcc
      181 tcggctaatc aagccgcagc agatctgctt tcgggcaggt ctgcttatcc acctcaggca
      241 atacctctca cagcaattag ttcttcttcc tcacttttcc gctacaatta tcaacaagtt
      301 gaatcgataa gaggcggtaa tgcgtcagac taaaaccggt atcctgctgg caaacctggg
      361 tacgcccgat gcccccacac ctgaagcggt aaaacgctat ctgaaacaat ttttaagcga
      421 cagacgcgtg gttgatacct cacggttgtt atggtggccg ttgctgcgcg gcgtgatttt
      481 gccgctgcgc tcgccgcgtg tggcgaagct gtacgcctct gtctggatgg aagacggctc
      541 gccgctgatg gtttacagcc gccagcaaca gcaggcgttg gcacaacgtt taccggatac
      601 gccagtggcg ctgggcatga gctacggctc gccatcactg gaaagctccg tcgatgaact
      661 cctggcagag catgtagatc atattgtggt gctgccgctt tatccgcaat tctcctgttc
      721 tacggtcggt gcggtatggg atgaactggc acgcattctg gcgcgcaaac gtagcattcc
      781 ggggatatcg tttattcgtg attacgccga taaccacgat tacattaatg cactggcgaa
      841 cagcgtacgc gcttcttttg ccaaacatgg cgaaccggat ctgctgctgc tctcttatca
      901 tggcattccc cagcgttatg cagatgaagg cgatgattac ccgcaacgtt gccgcacaac
      961 gactcgcgaa ctggcttccg cactggggat ggtaccggaa aaagtgatga tgacctttca
     1021 gtcgcgcttt ggccgggaac cctggctgat gccttatacc gacgaaacgc tgaaaatgct
     1081 cggagaaaaa ggcgtaggtc atatacaggt gatgtgcccg ggctttgctg cggattgtct
     1141 ggagacgctg gaagagattg ccgagcaaaa ccgtgaggtc ttcctcggtg cgggcgggaa
     1201 aaaatatgaa tatattccag cgcttaatgc cacgccggaa catatcgaaa tgatggctaa
     1261 tcttgttgcc gcgtatcgct aaagctgagc ggtaaagaac tgagcgccgt cgcgaagagc
     1321 ctcgtcggcg gttttcatca tccgtgaata atgcaaaaag gcatgcagcg tacctgggta
     1381 gagtttgaac tcacagggct gctgatgcgc cgctaacgtc tagtaaagca gacggctgtc
     1441 atccagcagc ggatcgaact ccgccccggc aataaaacag ggcggaactt cgcgagtgag
     1501 atcattatta aacagacagt aatacggtga ctcgcggtcc gcgtcgttgc ttaaatacgc
     1561 ctcttcatac atctgcaaat cctgttgcgt taagccatcc cagacaccgc ccaacagacg
     1621 acgagtcacg gaatcccgta atccgtaaag cccataccac agcaaaacac ccgcaatttt
     1681 accgcaatcg atctgtttat cacgcaacca caacgcactg gcgagcgcca gcatggcacc
     1741 tgcggaatca ccggcaaagc caatgcggga catattgatt tgataatcct ccgcctgctg
     1801 gtggaaataa caacaagcag ccacaatttc ctctatcgct tgtggaaaac gcgcttcagg
     1861 tgaaagggtg taattaatac caatcaccgt acattggctg tagcttgcca gcaggcgcat
     1921 gatgcgatcg tgggtatcga gattgccgag aataaaaccg cctccatgca aataaaatag
     1981 cgtcgccggg ctatctggct gcggacaaaa gagacgtgtt tccacctgcc catattttgt
     2041 tggaaccatg taagctctgg ttgccatttc tggagcgccc gcattccaga atcggcgctc
     2101 aagcgtgtaa tactgtcgtt gctcagcaat cgttcccgtt gcgggccagg acggtaaatc
     2161 cggctgaaga gtattcacaa cggtcttcat ttcagcagaa ataaggtcca gaacaggtag
     2221 tttgttttcc ggcttcatac aaaaactcct ttcaaattac gtcattgtaa ggaaccactg
     2281 ccatgaaaat gcgatcccgc ctgctgatat tgaaactggc tgcgtctcgc gcgctcccgt
     2341 cagattgtgt taacattcgc cgctcagtta accacccgta aaaacaacca tgaaatttcc
     2401 cggtaaacgt aaatccaaac attacttccc tgtaaatgca cgcgatccgc tgcttcagca
     2461 attccagcca gaaaacgaaa ccagcgccgc ctgggtcgta ggtattgacc agacgctggt
     2521 cgatattgaa gcgaaagtgg atgatgaatt cattgagcgt tatggattaa gcgccgggca
     2581 ttcactggtg attgaggatg acgtagccga agtgctttat caggagctaa aacagaaaaa
     2641 cctgattacc catcagtttg cgggtggcac cattggtaac accatgcaca actactcggt
     2701 gctcgcggac gaccgttcgg tgctgctggg cgtcatgtgc agcaatattg aaattggcag
     2761 ttatgcctat cgttacctgt gtaacacttc cagccgtacc gatcttaact atctacaagg
     2821 cgtggatggt ccgattggtc gttgctttac gctgattggc gagtccgggg aacgtacctt
     2881 tgctatcagt ccaggccaca tgaaccagct gcgggctgaa agcattccgg aagatgtgat
     2941 tgccggagcc tcggcactgg ttctcacctc atatctggtg cgttgcaagc cgggtgaacc
     3001 catgccggaa gcaaccatga aagccattga gtacgcgaag aaatataacg taccggtggt
     3061 gctgacgctg ggcaccaagt ttgtcattgc cgagaatccg cagtggtggc agcaattcct
     3121 caaagaccac gtttctatcc ttgcgatgaa cgaagatgaa gccgaagcgt tgaccggaga
     3181 aagcgatccg ttgttggcat ctgacaaggc gctggactgg gtagagctgg tgctgtgcac
     3241 cgccgggcca atcggcttgt atatggcggg ctttaccgaa gacgaagcga aacgtaaaac
     3301 ccagcatccg ctgctgccgg gcgctatagc ggaattcaac cagtatgagt ttagccgcgc
     3361 catgcgccac aaggattgcc agaatccgct gcgtgtctat tcgcacattg cgccgtacat
     3421 gggcgggccg gaaaaaatca tgaacactaa tggagcgggg gatggcgcat tggcagcgtt
     3481 gctgcatgac attaccgcca acagctacca tcgtagcaac gtaccaaact ccagcaaaca
     3541 taaattcacc tggttaactt attcatcgtt agcgcaggtg tgtaaatatg ctaaccgtgt
     3601 gagctatcag gtactgaacc agcattcacc tcgtttaacg cgcggcttgc cggagcgtga
     3661 agacagccag gaagagtctt actgggatcg ttaagttatc gtcggttcgt aggccagata
     3721 aggcgttcac gccgcatctg gcatttggct ctcgatgcct gatgcgacgc tggcgcgtct
     3781 tatcatgcct acatattttt catattttac atccggcaac caccgtttac cccgtcacca
     3841 cttcacccgc cggtggcgtt tccagcagtt ccagcatagt acgggcaatt tcacgttcac
     3901 ccatcactac ctgattcgcg ccacgttcgg tgatatacgt cacttcatcg tcataatggg
     3961 cgcgggcgat aatctcaata tccgggtttt tcgcgcgggc agacgccaca atctcacccg
     4021 cttcataacc gttggggatc gtcaggatca gccattttgc acattccaga tgtgccagtt
     4081 gcataatttc ttcgttcgct gcattgccca atacagcgcg gaccccacgc tctcgcagct
     4141 catcaacacg ggttcgtgac gtctcaatca ccaccagcgg aatatcagag gcgagcaatt
     4201 tctcccccag caggctgcct acacgaccgt aacccaccaa tagcgcatgg ttgcaaatat
     4261 ccactgggat ctgtttctct tcttcgattg cctcttccag cgtctgctct tccagcgttt
     4321 cggtcttcgc cagatatttc tccagtagtg cgaacagtac cgggttgagc ataatcgaca
     4381 ggatcgcccc tgccagtacc aggttttgtc cggcctgcgg cagtaaattc aatgccattc
     4441 ccagtcccgc caggataaac gcgaactcac caatctgcgc caggctggcg gcgatggtta
     4501 atgccgtacg ttgggagtga ccaaacagac ggacaaggaa cagggccgcg accgatttac
     4561 caaacagaat aattgccagc gtcgccagca ctgccagcgg ttgctgaatc agaattaacg
     4621 gatcaaacaa catcccgact gagacaaaaa acagcaccgc aaacgcgtcg cgcaatggca
     4681 acgtatcgtg ggcggcacgg tgactcaatt cagactcgtt cagtaccatc ccggcaaaga
     4741 acgcaccgag tgcaaaggag acatcaaaca gctctaccgc accaaaggca atccctaacg
     4801 ccagcgccag cactgacagg gtaaacagct cgcgagaacc ggttgccgcg ctgcgtgcca
     4861 taatccacgg caccagacgg cgacctacca gcatcataat ggcgataaat gcgatcacct
     4921 tgccgatggt gatccccata tcgactgcaa gagtggcaaa gcccacatcg ccctgttcca
     4981 tcattcctgc cactgcgggc agcaacacca gcgtcagaac catcaccagg tcttccacaa
     5041 tcaaccaacc gatggcgatt tgcccacgct gactgtctat taattgccgt tcttcaagtg
     5101 cgcgcagtaa caccacggta ctggcggtgg aaagacataa gccgaacacg ataccggtca
     5161 ttaacgacca gcccagcacg gcggagagcg ccatacccag cagcgtcgcc acggctatct
     5221 gggcgatcgc gccgggaatg gcgatggact ttaccgccat caaatccttg agcgaaaaat
     5281 gcaaaccgac gccgaacatc agcaaaataa cgcccagttc agccagttcc ggcgcaagct
     5341 tggtatcggc aacaaagccc ggagtgaatg gtcctgccag cacacccgct aacagatatc
     5401 ccaccagagg agaaatacgt agtttattgg ccagcatgcc gaggataaag gcgagcacaa
     5461 ggccgccaac aatggtggtg ataagcgggg tggcgtgatg cattccgtct ccttttcctg
     5521 gtggttattg tccatttttg gctgggaaaa ccaaaattac aggtaatagt ttatgacaat
     5581 ttcattgatg atgtttatga ataattgttg aattttgcag aaaaatggaa ttagctgcaa
     5641 aaaaagcacg gttcggaaaa cagaaggcgg tttaacagag gaaaggctta tggcgactgt
     5701 aaaacgatgc agccaaagtt tgcctttggc tgcaatgaaa tcagtcttta tgccggttat
     5761 caggcaggaa tatggtcaac atccccaata gtggcaggaa agcacagatt ttatagacta
     5821 actcgatgct ggtgtgatcg gcgataagcc ccagaactgc cgctcccaga cctcccatgc
     5881 cgaaagcaaa accgaaaaag agtccagaaa ccataccgat acgtcccgga agtagctcct
     5941 gagcgtagac cagaatggca gagaatgccg aagcgaggat aaatccaata atcaccgtta
     6001 aaacccccgt ccagtgcagg cgggcgtagg gtaaaatcag cgtaaacggc gcaacgccga
     6061 ggatagagcc ccaaatcaca tatttccgtc caattttatc ccctacaggc ccgccgatca
     6121 ccgtacctgc cgcaacggca aacaggaagg caaacagatg aagctgggca ttctggatag
     6181 ataatccgaa tttttgcatc agataaaagg tgtaatagct gctgatgctc gccatataga
     6241 aatatttcga gaaaatgagg attaacagaa tgctgactgc cagtaccact ttgttgcgcg
     6301 gcagtggatt gattatcgtc gctttgggtt ttcctttatt cattcggtgc tgtgccgagt
     6361 accaacggct aatttgcgcc aacaccacga tcgccagcag tgccgcaagc acaaaccagg
     6421 caacgttgcc tttgccataa ggctcgataa tcaccgccgc cagcaagggt cccagggaac
     6481 tgccaaagtt gccgccgacc tgaaagatag attgcgccag gccatgccgc ccgccggaag
     6541 ccatacgggc tacgcgagaa gattccggat gaaagaccga tgaaccggta ccgaccagcg
     6601 ccgctgccag cagaactgcg ccaaaactgc ccgccagcgc aagcagcacc agaccactta
     6661 aggtaaagca catgccaatt ggcaacgacc acggcatcgg atatttatcg gtccagtagc
     6721 cgaccactgg ttgcagtagc gaagaggcga gctggaaggt gagggttatc atgccaatct
     6781 gcataaatgt cagagaaaat tctgactgaa gcagcggata aatcgccaga atcagcgatt
     6841 ggatcatgtc gttcagcaga tgtgagaggc tgatagcacc taaaatacca aacgatgttc
     6901 gggccttggt cgttgacgca gccgcgcccg ccacaggctg ggtttgttca ctcattgcca
     6961 taggaaagtc actttttcag ggttgcgatg taaagaatga tcttatttgt gattattacc
     7021 cgactaacat acctgtatgc gtcgtctgaa ggaagtctca acgccgaata cagaatttct
     7081 aatctggatg cagatttatc ttcaccggac gcagacttgt ctatgatgtt gcgtcatact
     7141 atttttcaac acgttgaaat caggtcaggg agagaagtat gaaattattg cagcggggcg
     7201 tggcgttagc gctgttaacc acatttacac tggcgagtga aactgctctg gcgtatgagc
     7261 aggataaaac ctacaaaatt acagttctgc ataccaatga tcatcatggg catttttggc
     7321 gcaatgaata tggcgaatat ggtctggcgg cgcaaaaaac gctggtggat ggtatccgca
     7381 aagaggttgc ggctgaaggc ggtagcgtgc tgctactttc cggtggcgac attaacactg
     7441 gcgtgcccga gtctgactta caggatgccg aacctgattt tcgcggtatg aatctggtgg
     7501 gctatgacgc gatggcgatc ggtaatcatg aatttgataa tccgctcacc gtattacgcc
     7561 agcaggaaaa gtgggccaag ttcccgttgc tttccgcgaa tatctaccag aaaagtactg
     7621 gcgagcgcct gtttaaaccg tgggcgctgt ttaagcgtca ggatctgaaa attgccgtta
     7681 ttgggctgac aaccgatgac acagcaaaaa ttggtaacct ggaatacttc actgatatcg
     7741 aatttcgtaa gcccgccgat gaagcgaagc tggtgattca ggagctgcaa cagacagaaa
     7801 agccagacat tattatcgcg gcgacccata tggggcatta cgataatggt gagcacggct
     7861 ctaacgcacc gggcgatgtg gagatggcac gcgcgctgcc tgccggatcg ctggcgatga
     7921 tcgtcggtgg tcactcgcaa gatccggtct gcatggcggc agaaaacaaa aaacaggtcg
     7981 attacgtgcc gggtacgcca tgcaaaccag atcaacaaaa cggcatctgg attgtgcagg
     8041 cgcatgagtg gggcaaatac gtgggacggg ctgattttga gtttcgtaat ggcgaaatga
     8101 aaatggttaa ctaccagctg attccggtga acctgaagaa gaaagtgacc tgggaagacg
     8161 ggaaaagcga gcgcgtgctt tacactcctg aaatcgctga aaaccagcaa atgatctcgc
     8221 tgttatcacc gttccagaac aaaggcaaag cgcagctgga agtgaaaata ggcaaaacca
     8281 atggtcgtct ggaaggcgat cgtgacaaag tgcgttttgt acagaccaat atggggcggt
     8341 tgattctggc agcccaaatg gatcgcactg gtgccgactt tgcggtgatg agcggaggcg
     8401 gaattcgtga ttctatcgaa gcaggcgata tcagctataa aaacgtgctg aaagtgcagc
     8461 cattcggcaa tgtggtggtg tatgccgaca tgaccggtaa agaggtgatt gattacctga
     8521 ccgccgtcgc gcagatgaag ccagattcag gtgcctaccc gcaatttgcc aacgttagct
     8581 ttgtggcgaa agacggcaaa ctgaacgacc ttaaaatcaa aggcgaaccg gtcgatccgg
     8641 cgaaaactta ccgtatggcg acattaaact tcaatgccac cggcggtgat ggatatccgc
     8701 gccttgataa caaaccgggc tatgtgaata ccggctttat tgatgccgaa gtgctgaaag
     8761 cgtatatcca gaaaagctcg ccgctggatg tgagtgttta tgaaccgaaa ggtgaggtga
     8821 gctggcagta atccgaaagt gccggatgtt tgcatccggc acaatgctta atcgcggcgg
     8881 gcgatatcag caaatttggc atcgaggatc tttgccagat cgcctgccgc cagttcgata
     8941 tccagtccgc gcttgccgcc ggaaacataa atagtggcaa attcttgtgc gggggcgtcg
     9001 ataatcgttg gcagacgttt tttctgcccc agtgggctaa tccccccaac caggtatccc
     9061 gtcgaacgct gcgcgaccat cggatcggcc atctcaactt tcttggcacc cagcgctttt
     9121 gctacttttt taagatccag ttgacctgcg accggcgtaa cggccacggc aaggtgtttc
     9181 atatcaccgt tcactgccac cagcagcgtt ttgtagacct gatccggatt caaacctaat
     9241 tttttgacga cttcatcgcc aaaattggtt tcagccggat cgtgctcgta ggtatggatt
     9301 tgaaacgaaa tcttgttttt ttcgagtaat ttaactgcgg gtgtcatggt aattttcctg
     9361 ccttaaacaa aaaaacgaag caagcatacg cctgatggcg gtccaaaaaa aagagtcatc
     9421 ttgcctaaga gtattggcag gatggtgaga ttgagcgaca atcgagttac accgtcgctt
     9481 aaagtgacgg cataataata aaaaaatgaa attcctcttt gacgggccaa tagcgatatt
     9541 ggccattttt ttagcgcaac atttgcggca aattcccttc tccatacagg tgtagtgcac
     9601 cgaccgcgac cacatatcgc cccggcggca tggcgcgtaa tttatcccgc caggcgagat
     9661 ttcgctgatg catcagcaca tcgtacagcg actgactgaa cgtattgggc agcgttatat
     9721 cattattttg cggcggtgca ttcagccacc agctcatcat ttgttgcagc aaccgtgcgt
     9781 tggtatgcca gtgggtcagc gtatcgtcca gcagcgccag tcctttgtca gggagctgga
     9841 gcaacatggc aatctggttt tcagcccctt ccagttcaat cacgggttta tgttgttgct
     9901 tcgccgcctg caatagctgg taatcgatac cgtattctgc ccgcagcccc agtttttgtg
     9961 cctgcgtcgc ctgaagaacc atcgcgattt gccacagcgg ttgggtagaa aagagtgagg
    10021 gagaaatgcc catctcctgg ctaatgtgct gcaggttttg gagttgctcc tcgctaatgc
    10081 gctcttccag cgcctcgcag gcaggcaaat tagcaaaagg cgtatcgctg gtggaaacat
    10141 ccgcctcgac gatcagcgca tcggcgtttt tgagcttttt gagcaaacgg gtgggcaggg
    10201 gagccatatc gtggctaccc atatgaatac tgccaatcag atgaaaatgg cgattgccgg
    10261 ggagggtgat atcgatggca ggccaggtgt aatgattacc gcgcagcgcg gcccaaagcg
    10321 tttttacccg gtacaacaga tccatacgac ctccctttgt gaaatatcat gctagcgcgc
    10381 ggtgagggat ggcgcaaatg gagttgggga cttgtttaat gtgtttgtat gattcagtat
    10441 gttcttgcat cgctattcac agggaagcaa cagttaaaaa ccatgaaaca ggcaacaaga
    10501 aaaccgacga cacccggaga tattcttctc tatgaatatc tggaaccgct cgatttgaaa
    10561 atcaatgagt tagcagagtt gctgcatgtt catcgtaatc gcgtcagtgc actgatcaat
    10621 aacaatcgta aactcaccac tgagatggca ttccgtctgg cgaaagtttt tgataccaca
    10681 gtcgatttta ggctaaacct ccaggcggca gttgatcttt gggaagttga aaacaacatg
    10741 cgcacccagg aagaattggg acggattgaa acagtggctg aatatttggc acgccgtgaa
    10801 gagcgtgcaa aaaaggtcgc gtaaggccaa aacatcgctt catctcgtag gccggataag
    10861 gcgttcacgc cgcatccggc atccgagcac cattgcctga tgcgacgctt acgcgtctta
    10921 tcaggcctac aaacctgtgc ctgaaccgta ggccggataa ggcgtttacg ccgcatccgg
    10981 cagtcatgcg tcgatgccaa atgcgccgcc ctaaagcagc gcatccgcaa tgacgtactt
    11041 attccttcgg tttaaaccgc agcaaccggt tggcgttact tactacggta attgacgaga
    11101 gcgccattgc cgctccggca actaccgggt taagcagtgt tccagtaaac ggccacaaaa
    11161 taccggcggc gaccggaata ccgatgctgt tgtagataaa ggcaccgagc aggttctgct
    11221 tcatgttgcg cagcgttgcg cgggaaatcg cgagcgcatc cgcaacgccc atcaggctat
    11281 ggcgcatcag ggtaatcgcc gcggtttcaa tggcaacatc actgccgcca cccatcgcaa
    11341 tgccgacatc cgcctgggcc agcgctggcg cgtcgttaat gccgtcgccc accattgcca
    11401 cctgacgtcc ttcactttgc agacgtttga tcgcttcggc tttaccgtcc ggcagcactc
    11461 cggcaatcac ttcatcaatt ccagcttctt tggcgatcgc attggcggtg gttgggttat
    11521 ccccggtcaa catcaccaga cgatatcccg ctttatgcag gcgttgcagc gccgccacgc
    11581 tatcactacg caacggatcg cgtacagcca gcagggctac tgctttcccg tcaatcgcca
    11641 gcagcacagg cgttgctcct tgcgatgcct gagctgaaat atcggcttca atcgctttag
    11701 tatcgacttg ctgatcattt aacaacgcct gattacccag caataacgca tgaccttctg
    11761 cttcaccgct cacgcccagc ccgcgcaatg tgcggaaacc gttgacctgc ggtagctgca
    11821 tatcgcctgc tttatcaagg atcgctcgtg ccaatggatg gctggaacct tgctccagcg
    11881 ccgccgccag acgcagcgcc tgcgcttcgt caacatcagc aaatgttttc actgcgacaa
    11941 cctgcggctt cccttcggtc agcgtcccgg ttttatcgaa cacgacggta tcgagtgtac
    12001 tggcgcgttg cagcgcgtcg gcgtcccgca ccagcacgcc aaactcagcc gcccgcccga
    12061 cgccggaaat aatcgacatc ggcgtcgcca gtcccagcgc acacgggcag gcgataatca
    12121 gcaccgtagt ggcaatcacc agggtataga caatctgcgg tgccggacca aagaaatacc
    12181 agattgccgc actgacaagc gcaataacca ctactaccgg cacaaatatg gctgagattt
    12241 tatccgccag ctgaccgatt tctggcttgc tgctctgggc ctggcgcacc atgcgaatga
    12301 tccttgatag cgtggtatgg ctgccaaccg cactggcacg aaacagcaca ctgccgtcct
    12361 gtaccactgt cccggcatgg acgctatcgc cttcgccttt ttgctgcggg attggttcgc
    12421 ccgtcagcat cgcttcatcc agccatgctt cgccctgggt aatctcgcca tctaccggca
    12481 cgcgatcgcc ggtcgtcagg cgcagcaaca tacctggctg cacttctgcc agaggcacgc
    12541 ttttttcacc ttcgtcagta accaggcgcg ccgtcggtgg ggttaaatcg agtaatttct
    12601 ccagtgcctt agaagaacgc tgacgtgcgc gtgcttccag catatggccg agattgatca
    12661 gaccgataat catcgcactg gcttcatagt aaagatgtcg cgcttccatc gggaaccact
    12721 gcggccacag gttgacgctc atcgaataga gccacgccac gcccgttccc agcgccacca
    12781 gcgtatccat cgtcgctgca ccgttcagca ggcttttcca tgcactgcgg taaaaatggc
    12841 cgctggcgaa aaccatcacc gccagggtta tcaggccgat aaccaaccac aggctgcggt
    12901 tgtcggcggt gactatcatg ttatcgccga tcatccccca gaccatcacc gggataccca
    12961 ccgccagtgc gacaattgcc tgccagcgga agcgcttcat cgtagcgacg gcggtttctt
    13021 gctggcgttc gcggcgtttc gcgtcatctt caatcgcttc cgcgccgtag cccgcttttt
    13081 ccaccgcctg cactaaatct tgtggggagg cactgcccat caccagcgca gtacgctccg
    13141 ccaggtttac ccgtgcctga gtgacgcccg gtacgctttg cagcgcattt tgtacgcggg
    13201 tgacacagct ggcgcagctc atgccgctca gcagcaactg ctggctgtca tcgtcatcgg
    13261 cggtcgctgc cggaagcgcc tcagaaaccg ctgtcagtgc ttccgacggg attgatgact
    13321 ccgccagcgg tttagccttt gggtggctta cagatgcgtc ataacccgct tgtttgatgg
    13381 tttcgatcag ctgttctgca ctggcagtcc cggtaacgtg cgcttcagtg atagacacat
    13441 ccgcttgttc cacatccggg cgctgttcaa gactttcttt cacgcgttta acgcagtgac
    13501 cgcaggacag gccgtccagg ttcaggtcga tagtttgtga cataaaacac tcctttaaga
    13561 cagttttgac tggctgtgat aaaggttaaa ccttccagca aggggaaggt caagaaatta
    13621 ataaaccagg cgggtaaaag tccgtaaaga ttaaaaaatc ggctcgattt gcatcaggat
    13681 tagacattta tttctttgtt ttcctgtagt taagttgcgg gtgctaagtt aaatcaggat
    13741 gcctgaaaat cggcactggg gtgaggaatt acctcccgca tctataaaaa ggagttaaca
    13801 aaagatgtta gatgcaaaca aattacagca ggcagtggat caggcttaca cccaatttca
    13861 ctcacttaac ggcggacaaa atgccgatta cattcccttt ctggcgaatg taccaggtca
    13921 actggcggca gtggctatcg tgacctgcga tggcaacgtc tatagtgcgg gtgacagtga
    13981 ttaccgcttt gcactggaat ccatctctaa agtctgtacg ttagcccttg cgttagaaga
    14041 tgtcggcccg caggcggtac aggacaaaat tggcgctgac ccgaccggat tgccctttaa
    14101 ctcagttatc gccttagagt tgcatggcgg caaaccgctt tcgccactgg taaatgctgg
    14161 cgctattgcc accaccagcc tgattaacgc tgaaaacgcc gaacaacgct ggcagcgaat
    14221 tttacatatc caacagcaac tggctggtga gcaggtagcg ctctctgacg aagtcaacca
    14281 gtcggaacaa acaaccaact tccataatcg ggccattgcc tggctgctgt actccgccgg
    14341 atatctctat tgtgatgcaa tggaagcctg tgacgtgtac acccgtcagt gctctacgct
    14401 catcaatact gttgaactgg caacgcttgg cgcgacgctg gcggcgggtg gtgtgaatcc
    14461 gttgacgcat aaacgcgttc ttcaggccga caacgtgccg tacattctgg ccgaaatgat
    14521 gatggaaggg ctgtatggtc gctccggtga ctgggcgtat cgcgttggtt taccgggcaa
    14581 aagcggtgta ggtggcggta ttctggcggt cgtccctggc gtgatgggaa ttgccgcgtt
    14641 ctcaccaccg ctggacgaag aaggcaatag tgttcgcggt caaaaaatgg tggcatcggt
    14701 cgctaagcaa ctcggctata acgtgtttaa gggctgatca tgatgaacac ggaaggtaat
    14761 aacggtaaca aacccctcgg tctatggaac gtcgtttcca tcggtattgg ggcaatggtg
    14821 ggggcgggga tcttcgcgct gctggggcag gctgcgttgc taatggaagc ctcgacctgg
    14881 ggcgcctttg cttttggcgg tattgtggct atgttttccg gttatgccta tgcgcgtctg
    14941 ggggcgagct atcccagcaa tggcggtatt atcgactttt ttcgtcgcgg attaggcaac
    15001 ggcgtctttt cgctagcgct ctcgttgcta tacctgttga cgctggcggt gagcatcgcc
    15061 atggttgccc gtgcttttgg cgcttatgcc gtgcagtttt tgcatgaagg cagccaggag
    15121 gagcacctta ttttgctcta cgcgttgggg atcattgcgg tgatgacgct tttcaactcc
    15181 ttaagcaacc atgcggtagg gcggctggaa gtgatcctcg tcggcattaa aatgatgatc
    15241 ctgttattgc tgattattgc cggtgtctgg tcgctgcaac cggcgcatat ttccgtctct
    15301 gcgcccccca gctccggtgc gttcttctcc tgtattggga taaccttcct tgcctatgcg
    15361 ggctttggca tgatggcgaa cgcggcggat aaagtgaaag atccgcaggt cattatgcca
    15421 cgggcgtttc tggtggcgat tggcgttacc acgttgcttt atatctcgct ggcgctggtt
    15481 ttgcttagcg atgtatcggc attagagtta gaaaaatatg ctgataccgc cgtagcgcag
    15541 gctgcttctc cgctgctcgg gcatgtgggt tatgtgatcg tcgtcatcgg cgctttactg
    15601 gcgacggctt caggcattaa cgcgaacctg ttcgccgtgt ttaacatcat ggacaacatg
    15661 ggcagcgaac gcgaactgcc gaagctaatg aataaatccc tgtggcggca gagtacctgg
    15721 ggcaatatca tcgtcgtggt gttgataatg ctgatgacgg cggcgctaaa tttagactca
    15781 ctcgccagcg ttgccagcgc cacctttttg atctgctatc tggcggtgtt tgtggtggcg
    15841 atccgcctgc gtcatgatat tcacgcctcg ttgccgattc ttatcgttgg tacgttggtg
    15901 atgttgttgg tgatcgttgg ctttatctac agcctgtggt cccagggcag ccgtgcgttg
    15961 atatggatta ttggtgcaat cttactcagc cttattgtgg caatggtcat gaagcgtaat
    16021 aaaaccgtat aacacctctc tgtgcgcagt acttcctgta ttattgtggt ggcggtcgat
    16081 attcgcactg gcaaaaaaac gtgcttgaat atctgttgaa accctttaac aaagcacagg
    16141 aggcgttgcg cgaacgatga acatcagcga tgtagcaaaa attaccggcc tgaccagcaa
    16201 agccattcgc ttctatgaag agaaggggct ggtgacgccg ccgatgcgca gcgaaaacgg
    16261 ttatcgcacc tacacgcagc agcatctcaa cgaactgacc ttactgcgcc aggcacggca
    16321 ggtgggcttt aacctggaag agagcggcga gctggtgaat ctgtttaacg acccgcagcg
    16381 gcacagcgcc gacgtcaaac ggcgcacgct ggagaaggtg gcggagatcg aacgacacat
    16441 tgaggagctg caatccatgc gcgaccagct gctggcactg gcgaatgcct gccctggcga
    16501 tgacagcgcc gactgcccga ttatcgaaaa tctctccggc tgctgtcatc atcgggcagg
    16561 gtgattaaga cgagacggct cggatatgta gggttatccc ttctatcgca atgacttcaa
    16621 catgcgtacc tgcaccgaga tcctcgctgg cgctgacagg ccatgaactg tcaccgacgc
    16681 gcatatgacc gcgcccgttg accagcggag attccagcac aaaacgtcgg ccaatcagct
    16741 gctgcccgcg ctggtttaaa tgactatcgc tgtgcttttg ttcgcgcacc cgccgcgaca
    16801 accatttcca ccacagccag gcggcgagca gcgtcaggat ggcaaacatt accccttgcc
    16861 actcccaacc cagcggcacc agccagacca ccaggccggt aatcaccgct gccacgccgc
    16921 tccacaacaa ataaccattt ccgcccagca tctcggctgc cagcagcaac ccgccgagac
    16981 tgagccagaa aatatgtgga tgaacgacca ttaactccat catggctgag tccgtttgtt
    17041 ggcgctgtct ttcaccagct cggcaatccc ggcaatcgac cccatcaggc tgctggcctc
    17101 taatggcatc atcactactt tgctgttact ggaggaaccg atctgctgta acgcttcggt
    17161 gtatttttgc gctacgaagt agttcaccgc ctgaatatca ccggaggcga tagcttcaga
    17221 caccattttg gtggcgcggg cttctgcttc ggcggaacgt tcacgcgctt cagcctgtaa
    17281 aaacgccgac tgacgttcgc cttccgcttt caggatttgc gactgttttt caccttcggc
    17341 tttgaggatt tccgcctgac ggatcccttc cgcttcaaga atgtaagcgc gtttggtacg
    17401 ttccgctttc atctgcgcgt tcattgaaga gataagctct gccggtgggc gcacgtcgcg
    17461 aatttcaata cgggtgactt taatccccca cgggttggtg gcttcatcga caatacgcag
    17521 caggcgtgag ttgatgctgt cgcgctgaga gagcatttcg tcaagttcca ttgaacccaa
    17581 cacggtacgg atgttagtca tggtcaggtt gatgatcgcc agctccagat tgctgacttc
    17641 ataggcagcg cgtggcgcgt caatcacctg aataaaacag actgcgtcga tggtaacgtt
    17701 ggcgttatct ttcgagataa cttcctggga agggatatcg agcacttgct ccatcatatt
    17761 gatcttgcga ccaatgcgat ccataaacgg caccaccaga ctgagccccg gctgtaacgt
    17821 tttggtatag cgaccaaaac gttctaccgt ccattgatag ccctgcggca cgattttgac
    17881 gcccgcgccg acaatgacca gcgcgacaaa aatgagaatc gggataaaga taagcatcgg
    17941 aaaaacctcc tgttgtaccg tccataatca gcaaaattgc tgcttgatta aacaaattat
    18001 acctgattac tgaaagagag ttccccctta ttcctgcgaa ggataaactg tttttagtaa
    18061 aaatcagaaa aagggaacag cgatgcagga aaatagtcct ttgcttcagc tacaaaacgt
    18121 aggatatctg gcgggtgata cgaagattct taataacatc aatttttcgc tgcgtgctgg
    18181 cgaatttaag ttaattaccg gtccttctgg ttgtggcaaa agtacgctgc taaaaatagt
    18241 tgcttcattg atcagcccaa ccagcggaac gttactgttt gaaggtgagg atgtcagcac
    18301 actaaaacca gaaatctacc gccagcaagt ctcttactgc gcccagacgc cgacgctgtt
    18361 tggcgatacg gtatacgata atctgatctt tccctggcag atccgtgacc agcagcctga
    18421 cccggccatt tttctcgatt ttctcgaacg cttcgctttg ccggacagca ttttgacgaa
    18481 gaatatcgcc gagctatctg gtggtgaaaa acaacgcatc tcattgattc gtaacctgca
    18541 atttatgccg aaggttttat tgctggatga aataaccagt gcgctggatg aaagtaataa
    18601 acataacgtc aatgagatga tccatcgtta tgtgcgcgag caaaatattg ccgtgctgtg
    18661 ggtgacacac gataaagacg aaattaatca tgcggataaa gtgattacac tgcaaccgca
    18721 tgccggagaa atgcaggaag cacgctatga actcgcataa tattactaac gaatcattag
    18781 cactggcatt aatgctggtg gtggtggcaa tcttaattag ccataaagaa aaactggcgc
    18841 tggagaaaga tattctatgg agcgtcgggc gagcgataat tcagctgatt attgtcggct
    18901 atgtgctgaa gtatattttc agcgtggatg atgccagcct gacattattg atggtgttat
    18961 ttatctgctt taatgcggcg tggaacgcgc aaaaacgcag taaatatatt gctaaagctt
    19021 ttatctcatc gtttattgcc attacggtcg gggcgggaat taccctggcg gtgctgattc
    19081 tctcaggggc gattgaattt atcccgatgc aggtgatccc tgtcgccggg atgattgccg
    19141 gtaacgccat ggtagcggtg gggttgtgtt acaacaattt agggcaacgg gtcattagcg
    19201 aacagcaaca gattcaggag aaactgagtc ttggcgcgac gccgaagcag gcttcagcga
    19261 tattgattcg cgacagtatt cgcgcggctt taattccgac ggtcgattca gcaaaaacgg
    19321 ttggcttagt gagtttacca ggaatgatgt ccgggctgat atttgccggg attgatccgg
    19381 tgaaggcgat taaatatcag attatggtga cctttatgct gctctcaacc gccagcttgt
    19441 cgaccattat tgcctgctat ttaacctatc gtaagtttta taattcgcgc caccagttgg
    19501 tggtgactca attgaagaag aaatgatgcg atgccggatg cgcagcatcg catccggcgt
    19561 tgtggttcat gtgccgaaca acggaatatc aatacaacaa tgcatacagc tggcggcgat
    19621 acttcgacgc cagtgcatca cccgtaccca gcgcagcgag gatctcctgg aacgttttac
    19681 gcgtctgacc gtctgcggcg gtgagatctt tacgcagatg cccgaacagc aactccagcg
    19741 cctcttcatt gcgcccaacc tgatgcagtt gcagcgccag ttgcgtcgcc agtgcggcat
    19801 cttctggatt ctccgccacc tgctgttgca actgttgaat ttccggctta tcagccgcct
    19861 gcttcagcag ttcgatttgc gctaccagcc cctggtagcg ggtgtcctga tcctgcaacg
    19921 gaatggtttt cagcaccgct tccgcatctt cagaacggtt cagcgcaatc agcgtttctg
    19981 ccagcagcag gccgatctcc ccgttctgat tcgacaactg ccaggcgtct ttcagcaatg
    20041 gcagggcatc ggtgtaattg ccttcctgca tcagttgcat cgcctgctgc gctttcagct
    20101 cttcttcgcg cggcagcact ttatccagca gggcgcggat cgcctcttcc ggttgcggcc
    20161 cctggaagcc atctaccggt tgcccgttct ggaacagata cacggtcgga atcgcgcgca
    20221 gaccaaactg ggcggcaatc atctgctccg cgtcgcagtc cagcttcgcc agaataaatt
    20281 gcccgttgta ctgcgccgcg aggctttcca ggatcggggt taactgcaaa cagtgctggc
    20341 tacgttcaga ccaaaaatag aacagcaccg gagtggtcat cgactgttca agaacctgtt
    20401 gcaggttaga ttcgttaatg ttgacaatat tttctacgga catggagtcg ctctctgttg
    20461 tcgatatttt ctttgacatg ggggcttaag cgcgcgcttc aactcacccc tgcaatattt
    20521 tgtccatcac gcgcccaggc agcaggcgct cgagcaccat tactgcccag gtcaccagcg
    20581 ttaccggata gcgcatcttc ggcttctcgc taataaaagc atggcgcact ttgtcgacca
    20641 ccgcttccgg tcccaacgta aagcgggcgg cgatgccggg attttcgact ggtttatcac
    20701 tttgcgtctg gttgacgttg tcggtgaagc gagtacgaat gggaccgggt tcgatcaggc
    20761 tgactttaat tccgctgtgg cgtagctcca tgcgcagcgc gtctgaccac gcctccagcg
    20821 catatttact ggccgcgtaa gcgccacgac ccggcgtgga gattaatccc atcaccgatg
    20881 atgtcatcac aatacgccct tcaccgtgcg gtaacatcgc gggtaacagg cgcatggtga
    20941 gctggtgtgc gccgaaaaag ttggcggaaa actgctgttc catctgcgca cggctgatgg
    21001 tggaaagggg gccatacatg ccgaatccgg cattgttaaa gatcccatac agacaattat
    21061 cggtcagggc gatcacctcg tctgctgcgc gattaacact ttctggtgaa tccagatcga
    21121 tcaacacgcc ggtaaatccc atgttgttca tacgctcaac atcattcggt tttcggcaac
    21181 ctgccagcac atgaaaaccc tggcgtttta attcgagcgc gctttccagg ccaattccac
    21241 tggaacatcc ggtaattaag accgattttt gcataacttt acctgtcagg atctccgttg
    21301 ctttatgagt catgatttac taaaggctgc aactgcttcg ccatccagtc ggcaataaac
    21361 ggctgggcgt cgcggttggg atgaataccg tcatcctgca tccattgtgg cttgaggtag
    21421 acctcttcca taaaaaaggg cagcagcgga acatcaaact ctttggcgag tttggggtaa
    21481 atggcgctaa aggcttcatt ataacggcga ccatagtttg caggcagacg tatttgcatt
    21541 aacaatggtt cagcgttggc ggctttgaca tcctgcaaaa tctggcgcag cgtttgctcg
    21601 gtttgctgtg gctgaaaacc acgcaaacca tcattgcgcc cagttcaacc agcacccaac
    21661 gcggctgatg ctgtttgagc agagccggaa ggcgcgccag cccttgttgc gaggtgtcgc
    21721 cgctgatgct ggcattaact accgacgttt tactctgcca cttatcattc aacaaggcag
    21781 gccaggccgc gctggcagac attcgatacc cggcgctcag gctatcaccc agaatcaata
    21841 acgtgtccgc tgcggcggca cggaaggtta acaggaccag gaacaggaag ggcaaatgcc
    21901 agcggaaaac attgttgaag ttcatcatct taagaagtcc gtcggtcagg gggagcatga
    21961 actctccatc ctcaccggag ttgagctggt tgtcaaacgt ggcgagacca tcgcactggt
    22021 gggcgagtcg ggatcgggta agtcaacctt gctggcgatc ctcgccgggc ttgatgacgg
    22081 cagcagtggc gaagtgagtc tggtgggaca accgctacat aatatggacg aagaagcgcg
    22141 ggcaaagttg cgcgcgaagc acgtcggctt tgtttttcag ttatttatgt taattcctac
    22201 ccttaacgcg ctggaaaacg tcgagcttcc ggctctgctg cgcggtgaga gtagcgcgga
    22261 aagtcgtaac ggggcgaaag cgttgctcga acagttaggg ctgggtaaac gtctggatca
    22321 tcttccggca cagctttccg gcggtgaaca gcaacgagtg gcgctggcac gagcctttaa
    22381 tggtcgacct gatgtgctgt ttgccgacga acccaccggc aaccttgacc gccagacggg
    22441 cgataaaatt gccgacctgc tgttttccct caaccgtgaa catggcacca cgttgattat
    22501 ggtgacccac gacctgcaac tggcggcacg ctgcgaccgc tgcttacggc tggtgaacgg
    22561 gcagttgcag gaggaagcat gattgcacgt tggttctggc gcgaatggcg ttcgccgtcg
    22621 ctattaattg tctggctggc gctaagcctg gcggtggcct gcgtgctggc gctgggcaat
    22681 atcagcgatc gcatggagaa gggcttaagc cagcaaagcc gtgagtttat ggcgggcgat
    22741 cgggcgttgc gcagttcacg cgaagtgccg caagcgtggc tggaggaagc gcaaaagcgc
    22801 ggcctgaaag tcggcaagca gctgactttc gccacaatga cctttgcagg cgacacaccg
    22861 cagctggcga acgtcaaagc ggtggatgat atctacccga tgtatggcgg tctgcaaact
    22921 aatccccctg gcctgaaacc gcaggcgggc agcgtattgc tggccccacg cctgatggca
    22981 ctgcttaacc tgaaaacggg cgacaccatt gacgtgggcg atgccacctt gcggattgcc
    23041 ggagaagtga ttcaggaacc ggattccggt tttaacccct tccagatggc tccgcgtctg
    23101 atgatgaatc tggcggatgt cgataaaacc ggagccgtgc agccggggag tcgggtcacc
    23161 tggcgttata aattcggcgg caacgagaac cagctcgacg gctatgagaa atggttgtta
    23221 cctcagctta aacccgaaca acgctggtac ggtctggaac aggacgaagg cgcgctgggg
    23281 cgatcgatgg aacgctcgca acagttcctg ctgctttcgg cgcttctgac cttgctgctg
    23341 gcagtggcag cggtggcggt agcgatgaat cattactgtc gcagtcgcta cgatctggtg
    23401 gcgatcctca aaacgctggg ggcagggcga gcgcaactgc gtaagctaat cgtcggtcag
    23461 tggttgatgg tgctgacgct ttcagccgtt accggtgggg ccataggcct gttgttcgaa
    23521 aacgtgttga tggtgctgct caagcccgtt ctgcctgctg cactaccgcc agccagcctc
    23581 tggccgtggc tgtgggcgct tggcaccatg acggtcatct cgctgctggt ggggctacga
    23641 ccataccgct tgttgctggc aacgcagcct ttacgcgtat tacgtaatga tgtggtcgcg
    23701 aacgtctggc cgctgaagtt ttatctgccg attgtcagtg tggtggttgt gctgctgctc
    23761 gccggattaa tgggtggcag catgctgctt tgggcggtgc tggcgggcgc ggtagtactg
    23821 gctttgctgt gcggtgtgct gggctggatg ctgctgaatg tacttcgccg catgacgctg
    23881 aaatcgctgc ctctgcgcct ggcggttagc cgcctgttac gtcagccgtg gtcaacgtta
    23941 agccagcttt cggcattttc gctctccttt atgctgctgg cactgctgct ggtgctgcgt
    24001 ggcgatctgc tcgaccgctg gcagcagcag ctaccgccag aaagcccgaa ctacttttta
    24061 attaacatcg ccacagaaca ggtagcaccg ctaaaagcgt tcctcgcgga acatcatata
    24121 gtcccggaat cattttatcc ggtagtgcgg gcgcggctga cggcgattaa tgataagccg
    24181 acagaaggta atgaagatga ggcgcttaat cgcgaactca atcttacctg gcaaaatacg
    24241 cggcccgatc ataatccgat tgtcgccggt aactggccgc caaaagccga tgaagtgtcg
    24301 atggaagagg ggctggcgaa acgcttaaac gttgccctcg gtgataccgt gacttttatg
    24361 ggcgataccc aggagttccg cgctaaagtg accagcctgc gcaaagtgga ctgggaaagt
    24421 ctgcggccta atttctattt tattttccct gaaggggcat tagacgggca accgcagagc
    24481 tggcttacca gtttccgctg ggagaatggc aacggcatgt tgacgcaact caaccgccag
    24541 ttcccgacca ttagcctgtt agacattggc gcgattttaa aacaggtcgg tcaggtgctg
    24601 gagcaggtaa gtcgggcgct ggaggtgatg gtggtgctgg tcaccgcctg cggtatgttg
    24661 ctgttgctgg cacaggtgca ggtgggaatg cgccagcgtc atcaggagct ggtggtatgg
    24721 cgcacactcg gtgcggggaa aaaactgctg cgcacgacat tgtggtgtga tgttcgccat
    24781 gcttgggttt gtttccggcc tggtggccgc aattggtgcg gaaacggcac tggcagtgtt
    24841 gcaggcgaaa gtgtttgatt tcccgtggga gccagactgg cgattgtgga ttgttctgcc
    24901 gtgcagcggt gcgctgctgc tgtcgctttt cggcggctgg ctgggtgcgc gactggttaa
    24961 gggtaaggcg ctgttcaggc agtttgtggg gtgatgacag gtgttagatt gcctgatgcg
    25021 ctacgcttat caggcctacg aagagcgtgc aatatattga ttttgcaaga ttgtgtaggc
    25081 cggataaggc gtttacgccg catctggcat gaacagtgtg cattttggca ataaatatga
    25141 aaccaactgt catgtcggtt tttttaaagg ccgaaggagg taaaccttcg gccttagcta
    25201 cgtttcctca gttattcaat aaactgacgg atcagttcca gcagcgcgat tagcgcctta
    25261 agaacgatga gaactttatc gagaaaagtt ttcatccgtt gctcccttaa gggagaaaga
    25321 caatcacagc ttacccttac gttgcctata actatgattt tcttcatgtg ctagatgagg
    25381 actgtctctc tccaaattag agcaccagaa tcagcaccac ctgtatcctg accaggactt
    25441 aaaagtactt ttccgcactg cggtaatccc ttgaggcagt atcactacta gcgtcgggat
    25501 tgtatcgcca acatagctgt ttgaatatgt aggcatcctg acttataatt cacttgctag
    25561 atgaggactg tttaatttct gcccggaacc accaagtctt gggcggaaaa aataaaaaaa
    25621 ccgactgtca tgtcggtttt tttatatctg tattctggtg caaccatttt gttctcacga
    25681 caatcgccgt ttcagaatac cgcccatcac tgcgccttaa cccataccgc cacttccgcc
    25741 cactcaccgc gaaagacaac tttttccgct tttttctcaa gctgatagcg atacattggg
    25801 tcgtaatatt ctttaagtaa cggcaccagc caggccagat gaccgtcggt gctgccggtg
    25861 gtgagttgcg ttgtcagcgc cgtatccagc tgggcagcga gttcgttata gcgctgtaac
    25921 cccagccgac gcttaatcgc cgaaagtccg tgatgcaggt attcgcaata ttcctgccag
    25981 ccctgttcgt cgccataagc gtgggtaaaa tcatgatgca tacgcaagaa atactcttca
    26041 ttcaggcgtt caagacggat ctcaaacgga tcttctacca ccgcaatcgc cgcctgagtc
    26101 attcgctcgc gcagacattc cggcaggtga ttcgaaccga tcatccggct ttcatcttcc
    26161 agcacccaca ggcgcaaatc ctgacgggcg tcggttttta gcatttcggc agccagcagg
    26221 ttttcaaaac tcgcctggct aagttgtggt tgtaacgtgc gaccaaacgc cgaaccgcga
    26281 tgacgcgcca gcccttccag atcaacaccg tttggctgtt gttgcactaa tagcgtttta
    26341 ccgctgccgg tacaaccgcc aatcagcact atcggttttt gtgccagttc aatggtcgcc
    26401 tgaatcgcgg tctggcgcag tgccttataa ccgccttcca ccagcggata atcaatcccc
    26461 gcttcatgca gccagcgttg cacaatatgt gaacgttgac cgccacgggc gcagcagaga
    26521 ataccttgcg gattttgcag gcacgctgcc cgccaggcgt acatacgctg ctgacgaatt
    26581 tcacccgcca ccagtttatg ccccagcgcc agcgctgcgt ctgagccttg ctgtttatag
    26641 caggtgccaa cggaggcgcg ttcatcgtta tccattaacg gcagattgat agcggcgggc
    26701 attgcgcctt gctcaaactc gataggggcg cgaacatcaa taatgggcgt atcagcaatc
    26761 agcagggcac gatagtcctg ttccgtgtgt ctctcttgca tagttaaaag tgaacctcaa
    26821 atcagcttgc gcgctatttt acgcgccaac gcgcaaggaa acttgatttt taactgcgtg
    26881 gggtgccgaa aatttctaaa aatccgctga tttccggcct gtgttgggta aacaaggtca
    26941 caatatcttc taccgctttg cagctgccaa atttgcgcca tgccagactc aatggcgaag
    27001 gagggcgcat cgttgggatt acccggctga ccagttgttg attatcgatc attgactggc
    27061 aaagtgattt tggcaaaaaa ccaatgccaa cacccgccag atgggcggcg attttcgttt
    27121 ccatatcggg aacaataatc tctttttgcc ccggcaatcg ccaggcgacg cgtttggtta
    27181 aggtgccggc gctgtcttca atattgaccg ccggaaagcg acgcaactgc gcttctgtta
    27241 gcggctcttc aacgttcgcc agcggatgat ccgccgccat gacaaatcgc cattgcaccg
    27301 atcctaaggg atcaagacta aaggtatttg ccagcgcctc agttcccgtg accccgatag
    27361 ccagcgaaaa accttcgtac aatagcgagt cccagacgcc catatagatt tgtcgggaga
    27421 tgtgaaactg ggtaaagggg taacgctcat tcagccacgc cagcaactgg gcgacggcct
    27481 gggggttata gagcaggttg ttgatgacaa tattcacctg gcgttccacg ccatcattca
    27541 cctgttgcag ctcgctgggc atactttcca gccagctcag ccagtctctg gcctgggaaa
    27601 gtagatgctc gccagccgct gtcaacgtta cgctacgagt cgtacggaaa aacagcgcta
    27661 ctccggtatt ctcttccaga agtttaatgc gataactgat cgtcgccgtg gttttacata
    27721 atcgttctgc cgcttttgaa aaacttcctg tttcagcaac cgcaatgaaa gtccgcaagg
    27781 tttctggatc gaacatcttc aggtatcccc ttttaaatcc gcaagttgcg tgattttctt
    27841 atcct
//
LOCUS       DAZOYF010000039        27399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZOYF010000039 DAZOYF010000000
VERSION     DAZOYF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.039"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..576
                     /locus_tag="ACHUOF_001579"
     CDS             <1..576
                     /locus_tag="ACHUOF_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0610809.1"
                     /translation="LAEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLC
                     MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLD
                     NLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEK
                     GSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            812..1630
                     /gene="ybhA"
                     /locus_tag="ACHUOF_001580"
     CDS             812..1630
                     /gene="ybhA"
                     /locus_tag="ACHUOF_001580"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFR0610810.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACHUOF_001581"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACHUOF_001581"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR0610811.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACHUOF_001582"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACHUOF_001582"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR0610812.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACHUOF_001583"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACHUOF_001583"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0610813.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACHUOF_001584"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACHUOF_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR0610814.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="ACHUOF_001585"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="ACHUOF_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR0610815.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="ACHUOF_001586"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="ACHUOF_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR0610816.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="ACHUOF_001587"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="ACHUOF_001587"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR0610817.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="ACHUOF_001588"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="ACHUOF_001588"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR0610818.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="ACHUOF_001589"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="ACHUOF_001589"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR0610819.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="ACHUOF_001590"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="ACHUOF_001590"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR0610820.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="ACHUOF_001591"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="ACHUOF_001591"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR0610821.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="ACHUOF_001592"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="ACHUOF_001592"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR0610822.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13936..14316
                     /locus_tag="ACHUOF_001593"
     CDS             13936..14316
                     /locus_tag="ACHUOF_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR0610823.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="ACHUOF_001594"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="ACHUOF_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR0610824.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="ACHUOF_001595"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="ACHUOF_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR0610825.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="ACHUOF_001596"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="ACHUOF_001596"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR0610826.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17417..17492)
                     /locus_tag="ACHUOF_001597"
     tRNA            complement(17417..17492)
                     /locus_tag="ACHUOF_001597"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="ACHUOF_001598"
     tRNA            complement(17496..17571)
                     /locus_tag="ACHUOF_001598"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="ACHUOF_001599"
     tRNA            complement(17623..17698)
                     /locus_tag="ACHUOF_001599"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="ACHUOF_001600"
     tRNA            complement(17701..17776)
                     /locus_tag="ACHUOF_001600"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17912..17987)
                     /locus_tag="ACHUOF_001601"
     tRNA            complement(17912..17987)
                     /locus_tag="ACHUOF_001601"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17952..17954),aa:Lys,seq:ttt)
     gene            complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACHUOF_001602"
     CDS             complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACHUOF_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR0610827.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACHUOF_001603"
     CDS             complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACHUOF_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR0610828.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACHUOF_001604"
     CDS             complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACHUOF_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR0610829.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACHUOF_001605"
     CDS             complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACHUOF_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR0610830.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACHUOF_001606"
     CDS             complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACHUOF_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR0610831.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACHUOF_001607"
     CDS             complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACHUOF_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR0610832.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACHUOF_001608"
     CDS             complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACHUOF_001608"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0610833.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="ACHUOF_001609"
     CDS             complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="ACHUOF_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR0610834.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="ACHUOF_001610"
     CDS             complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="ACHUOF_001610"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR0610835.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="ACHUOF_001611"
     CDS             complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="ACHUOF_001611"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0610836.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="ACHUOF_001612"
     CDS             complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="ACHUOF_001612"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR0610837.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         6898 a         7262 c         6706 g         6533 t
ORIGIN      
        1 cttgccgaag aagatccagc ttctgcaacc aggccattca gaactgaagt gaagcgcagt
       61 cgactgttaa ttgacgagta tctgttaatc aggaaaatag cagacgtcca gaacgaatgg
      121 ttcggccttt gcatggatct tgcactggta acaggacagc gcaaaggaga tctggcggct
      181 atgagatggg aggatataag agatagcagg ctatacgtgg aacaacaaaa aacaggggca
      241 aaaattcgca tttctctccc caccacgatt tccagattga atttaacact tgccgatgta
      301 ctggataact tgaaaaaaat aaacggtaaa aacgaaaagt tactcggagg gaagacagcc
      361 aggacaattg cagcacagtt caggattgcc cgagacacat ccggattgaa atgggaaggt
      421 gacccaccgc catttcatga aatccgaagt ttatccggtc gcctgcactc agcagaaaaa
      481 ggcagtgatt tcactcaggc gctccttgga catcgttcat ccagcatgac ggataaatac
      541 cgtgacggac gagggaggga atggaaagac atttagaatt aatttcgaat gatttcgaaa
      601 tgccacttgt aattaattga ttataaagaa ttaattccta aagagcattt gcttcatgaa
      661 tgctcctttg catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa
      721 tgctgaatta aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac
      781 gaaaattctt aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt
      841 agacggcacc ttattgaccc cgaaaaagac cttgcttcca tcatcgatag aagccctggc
      901 ccgcgctcgc gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat
      961 tcatcctttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattgccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc actcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag attacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggtc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgttt ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgcactct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagtgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaatgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggccga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tatgccaacc gcatcgccaa tagtcgctat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagcatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcaccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatgg gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgttgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctgga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctctcctaca atttctaact gtaactcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca caccaccatt cagatggaat
    15301 atcaaccttg tcataggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 gccgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggtca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgcttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgtaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcga attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttgcattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtgggggtcg
    17821 tgcaggatga ctcgacttcg tctcgccctt cggcgccgtt gctgatgcaa cgttatcctt
    17881 cacgttttac taccgcttcc accttagcga ttggtgggtc gtgcaggatt cgaacctgcg
    17941 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccact ttttcgttgc
    18001 tttcggtttg tttgatatcc cgtggcaacg gcggcatata ttactgattt cagacttgag
    18061 cgcaacaaaa atttcgatgc agatcactca actgcttatg attcgcacga cacgaccaga
    18121 aataatgcga cttctggtcg tgtgttatgc attacatcgc gttcagacgt ttttgtgcct
    18181 gtttagcgcc atcagtacca gggtatttac tgataacctg ctggtatacg gctttcgctt
    18241 ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt aaacatcgca tcagcagcct
    18301 ttggtgactt cggatagttt ttcactaccg aagcaaaata gtacgccgca tcatcttttt
    18361 tacccttgtt gtagtttaac tgacccagcc aataattggc gtttggcagg taagttgaat
    18421 cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc gtcatcctgg cgggatttat
    18481 cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc atcgccgctt ttcaccggcg
    18541 cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt tgatgccgtc gcaccgcttt
    18601 gatcgctgct ggttgattgc gccgctgcac caccgctgct gaggctgtcg atctgcaaca
    18661 ggatctgctt ttgccgctcc acgacctgat tcagttgata ctgattttcc tgaatctgac
    18721 cacgcaggga atcaatatcg gattgattat cagaaagttg ttgctggagt tgggttaaaa
    18781 gctggctgtg agcattagaa atacgctcaa gttgagtgac gcggtcttcg accgagcctg
    18841 agctgacact actgattggt gcctgagcaa aagcggccca gggggccgct ataccaacca
    18901 gtaacgacag actcaatagt tgatgtctga agttactgct catgcaattc tcttagtaaa
    18961 ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc atgacccagt actgcaggtt
    19021 tttctttacc gtaagaaacg atggagatct ggtctgcaga aacgccttta ccctgcaggt
    19081 acatcttaac ggcgttcgca cgacgttcac ccagggagat gttgtattcc ggagtaccac
    19141 gttcgtccgc gtgaccttct acggtgactt tgtaagacgg gttgctacgc aggaagtttg
    19201 catgtgcatc cagcatttga gcgaagtcag aacggatatc gtacttgtcc agatcgaagt
    19261 aaacgatgtt gttctgctgc agctgttgca tttgcagacg agcctgctct tcggaagaca
    19321 tgttgccgtt gccgccgttc gcatccatac cagtgccggc acccagcatg ccttcgctgc
    19381 cgtcattgct ggcgttcttg ttggaagaac atgccgcaat tgccataaca ggcagagcaa
    19441 tcatcagccc tttcagcact ttgttcagtt gcatttcaat gattccttta ctattcaatt
    19501 aattattatc acagatacgg cgaccaggca gggaatttga cctgtccatc agttgccgga
    19561 agacgcgctt tgaaacgccc atctgtagaa accaaattca gcacggatcc catcccctga
    19621 gaagagctgt agattaccat agtgccgtta ggtgccagac ttggcgtttc atccaggaac
    19681 gtggacgaca gaacttgtac gcctcccgtt gccagatctt gtttggcaat gtgctgctgc
    19741 ccaccattgg agctgaccat taccataaat ttaccgtcgc tgctgacatc cgcatcctgg
    19801 ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc cgttgatatt cactttataa
    19861 acctgcggac gaccggcctg gtcagaagta aatgccaggt tctggctatc cgggaaccag
    19921 gtcggttcgg tattgttact gcgaccatca gtcacctggc ggatctgacc agaagccaaa
    19981 tccattacgt acaggttcag actaccggtt ttcgacaagg cgaatgccag tttgctgccg
    20041 tctggcgaga atgcaggtgc accgttgtga cgcgggaatg aagccacctg acgtacagcg
    20101 ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc tttcgaaggt cacataagcc
    20161 agtttagaac cgtctggtga ccacgccgga gacatcagcg gctgcggtga acggtgaacg
    20221 acaaactggt tgtaaccgtc atagtcagat acgcgcagtt catacgggaa ctgaccgccg
    20281 ttggtctgaa caacgtaggc aatacgggta cggaacgcac ctttaatgcc ggtcagtttt
    20341 tcaaacactt catcactggc ggtatgacca gcataacgca gccactgctt gttcactttg
    20401 tacgagttct gagcaagtac agtacccggt gcgccgccag tgtcaacaag ttgataagca
    20461 acattgtaag aaccatccgg attcggagtg acttgaccga caactacagc gtcaatgccc
    20521 agtgcggacc atgcagctgg ttgtacttcc tgcgcactac ccggctgctg tggcagacga
    20581 gcgcgatcta acggattaaa tttaccgctg ttacgcaagt cagcagcaac gatgccgcca
    20641 atatcttcag gtgccgcacc aggccccgcc cactggaaag gaacaacacc aataggacga
    20701 ccggaatcta caccgctgtc gatcacaatg cggacttcag catgcagaac tgatgcccac
    20761 agtatgagaa aaccaaatgc tactcgtaat gcctgcttca tcatatctcc cttatctgga
    20821 ccgatggccc acgataattt agcagaatgt taacaaacta aaatacacaa aactaccaga
    20881 accccgtggc aaccggtgcc tgatgttgac cgtccgaaca gtcaacatcg cgattacggt
    20941 ttgaagtcca atggcgcgtt tttgaacact tcatatactg cctggcttgg tggtttcggg
    21001 atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa gtgcgggatc gccaccttca
    21061 ggtttgatat ccagtaacat accatcgggt gccagtttta tgcgcagcgt acaggttttg
    21121 cctgcatagg acgatgcgtc atagaactta ctttcgatag cagatttaat ctgcccggca
    21181 tagttattga tatcggcccc tgatgcgcca ttgtttttag tattaccact cccggcaggc
    21241 gaagcattgt tccctttcgc ccctcccccc gttttcggtg cattcttacc agagcttagc
    21301 tcaccgaaaa tatcatctgc ctctgcggca gcttttgctg cagcggcttt ttcagctgct
    21361 gcttttgccg ctgccgcttt tttgtctgcg gcggcttttt ctgccgctgc tttcttatca
    21421 gctgctgcct tatcagcagc cgctttttct gctgcttttt tgtcggctgc agctttctct
    21481 gctgccgctt tcttatcagc tgcagccttt tcagcagccg ctttcttctc agcttctgcc
    21541 ttggcttttt cagcagcttc agttgccgct ttctttcttg cttcagctgc agctgcttct
    21601 gccgcttccg ctttcttctt cagtgccgca gcggctacct cggctttttt ctgcgcttcg
    21661 gctgcggctt tggcggcttc tgcttctgct tttttcttcg cgtctgcagc cgctttcttc
    21721 gctgcttctt ccgcagcttt agcatctgcc gccgctttcg ctgccgccac ttcagcttgc
    21781 ttctgcttta actcggcctg ttttgcggct tcttcagcct gctttttctg ctcctgagcc
    21841 gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg tttctcacgc
    21901 agttcttcag cagcctgctg ttccttcatc ttgcgctgtt catcagaacg cttcgcgctt
    21961 gattcctggc tttgcatgcg tttgtactgc tcaactaccg cacctgaatc aaccatgaca
    22021 gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt ctatattctc atcgaacgaa
    22081 ctccagatca gcgccgcaaa taagatgaca tgcagcactg ctgaaataat tatcgcccgc
    22141 ttgagcttgt cgttttgttc ggttgccttt gacactctcg gtttccaaaa actgttcgcc
    22201 tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt tagataggct gcgtcattaa
    22261 accaaccgat ttcacacccg cactatgtaa caagttcagt gctttaatta tttcatcgta
    22321 aggcacatct tttgcgccac cgatcagaaa gaccgttttc gggttggcct tgaaacggct
    22381 ggacacttcc gccaccacct gctctggtgg taaacgctcc aggcgatctt tctcaaccac
    22441 cacggtgtac tgaccaatac cagacacttc aacaatcact ggcggattat cgttactgct
    22501 caccgcctgt gattcagtag cgtctggcag atcgacctcc acgctctggg tgatgatggg
    22561 cgctgtcgcc ataaagatca gcaacagcac cagcagtacg tccagcaacg gaacaatgtt
    22621 gatttcggac ttgagatcgc gacgacctcg tccacgcgct ctggccatgg cttacccctt
    22681 gttgctctcg ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact cttccataaa
    22741 gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg agacggttat aagccattac
    22801 cgccggaatt gcggcgaaca gaccaattgc cgtcgcaatc aacgcttctg cgataccggg
    22861 cgcaaccatt tgcagtgttg cttgttttac cgccccgagg gcgataaagg cgtgcatgat
    22921 cccccagacc gtaccaaaca gaccaatata cgggctgatg gagccaaccg taccgaggaa
    22981 cggaatgtgc gtttccagat tttcaagttc acggttcatg gagatacgca tagcacgcga
    23041 cgccccttcc actacggctt ccggcgcatg gctattggca cgatgcaggc gcacaaactc
    23101 tttgaacccg ctgtaaaaga tttgttccga accagtcaga ttatcccgtt tcccctgact
    23161 ctcttgatag aggcgagaaa gttcgattcc agaccagaat ttatcttcaa acgcttcggc
    23221 ttcacgcgcc gctgcgttaa gaatacgggt tcgctggata ataatggccc aggatgcgat
    23281 tgaaaaacca atcaaaatca acatgataag tttaaccaga aggctagcct tcaggaacaa
    23341 atcaaggata ttcatgtcag tcactgctta aactccgcga caatagactt gggaagcgca
    23401 cgaggcttca ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc attcagcaaa
    23461 gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg cattgatgtt
    23521 atttcagtct gtatttcgag catatcgtcg agccgcgcag gtgcgtaata ttccaccgtc
    23581 attttacgta ccacaaaggc aacgcgttca gccatcagcg cctgctgact gaagtgatga
    23641 tgacgcagca tctctgtgcg tgctctttca taaaaagcga cgtaactggc gtggtacacc
    23701 acaccaccgg catcggtatc ttcatagtag acgcgaaccg gccatcgaaa cagcgttgta
    23761 ttcactttac atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg ctactatacg
    23821 cgcgcgattg aggtttggga atgagggcaa gttaagggcg cgtaaaaatt tatgggccta
    23881 gagcggccca taatttttta aagagaattc tgatttaaaa gaagaagaaa atcagcccga
    23941 caatgagaac aatatcggca agcaatgggc aaaaaatccc ttgccaaaga accttctgcg
    24001 gacgaaagcc cacgccgtga atcacaccag cacagacggc ccacatcagc aataaaccat
    24061 gccagatttc cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa aacatacatc
    24121 ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg tccattaccg
    24181 catacaaggt cgcgataatc ttactcatca gatgtcttct tgaccggctt tgcctgattc
    24241 gacgtgttca agcgccagcg cggtgattac cccaaacgaa caggcaagaa gcgttcccag
    24301 aatccatgcg aaataccaca tttttagctc cttacttagt acagagagtg ggtgttacgt
    24361 tcaatatctt ctttggtgat acgaccgaac attttccagt aacaccaggc ggtgtagagc
    24421 agaatgatcg gtaccagaac caccgcaacc caggtcatga cgttaagcgt cagctggctg
    24481 gaagttgcat cccacattgt cagacttgcg ttcatcatgg tgctggacgg catcacaaac
    24541 gggaacattg cgataccggc tgtcaggatg atgcaggcca gcgtcaggga ggagaacaca
    24601 aacgcccacg cggctttatc catacgtgca gtcaggatgg tcagcagcag cagaaccaca
    24661 cccagtgccg gaatagccca cagaattggc gtgttgttga agttaaccag ccatgcgcca
    24721 gcttcacgaa ccacttcttt attcagtggg ttagaggctg cgtaatggtc cattgtcgat
    24781 ttcacgacat aaccatcgat accgtacatc acccatacgc cagccagtgc gaaacagacc
    24841 agtgtcacca gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag ttcgcccacg
    24901 gtacgcattt gcagataggt tgcgccctga gtaatgatca tccctacgct caccacgcct
    24961 gccagcaggc cgaacgggtt aagcaactgg aagaagttac cggtgtagta cagacgcaga
    25021 tattcatcaa cgttgaacgg tacgccctgc aacaggttac cgaacgctac accaattacc
    25081 agcggcggaa cgaagctacc aatgaagatg ccccagtccc acatgttacg ccagcgggtt
    25141 tcttcaatct tggagcggta gtcaaaaccg accggacgga agaacaaaga cgccagcacg
    25201 aggatcatcg ccacatagaa gccggagaac gcagcggcat agaccatcgg ccaggcagca
    25261 aagagtgcgc cgcccgcggt gatcagccaa acctggttac cgtcccagtg tggtgcaatg
    25321 gagttaatca taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt gagcatgccc
    25381 acccccatgt cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc aaccagcagc
    25441 caccagataa aacgcaatac ttcataatcg atcatttgac gactcctgtc ttagcgtgcc
    25501 ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac cggttttcag gctgcttggg
    25561 ccgaggcgtg caaacttgaa cattaagaac aattctgcca ccaggaacag ggtatacagg
    25621 ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg tcagtgacga attcgccaca
    25681 gctgtcggca gcacttcacc gatagcccac ggttggcggc catattcagc cacgaaccag
    25741 cccgcttcta cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg cagaagccat
    25801 tttttctcgc caatgcggtt gcggatgaca ctccagaaag agagcgcgat gattgccagc
    25861 agcaggaagc cacacgccac catgatacgg aacgcaaagt acagcggcgc tacacgcggg
    25921 atggagtctt tggttgcctg ttgaatctgc gcttcagtcg catcagccac gtttggcgta
    25981 tagcgtttca gcagcagacc gtaaccgagg tctttcttca tgctattgaa ctggtcacga
    26041 accgtctggt cggtagaacc agaacgcagt tgttcgagca gagagtacgc cttcatcccg
    26101 ttacgaatgc gttcttcatg ctgcaccatc agctctttca ggccgataac cggggtatcc
    26161 acggaacgcg ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa tttgttcgtc
    26221 tcctcttcct gatcaggaat gccgaacaaa gtaaaggcag caggcgcagg ttgcgtttcc
    26281 cactcggctt caatagcagc cagtttggtt ttctgcacgt cgcccatttc gtagccggat
    26341 tcatcaccca gaacaataac agacagaaca gcagccatac cgaagctggc agcgatagca
    26401 aaggagcgtt tagcgaaggc gaagtcacga cctttcagca tataccatgc gctgataccg
    26461 aggatgaaca tcgcgccagt cacataacca gacgctacag tgtgaacgaa tttcacctga
    26521 gcaaccgggt taagcaccag ctcggagaag ctcaccatct ccatacgcat agtttcaaag
    26581 ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg caaccagaat ccacagtgcg
    26641 gacaggtttg aaccgagcgc caccagccag gtgacacaca tatgctgaac tttacccaga
    26701 cgatcccaac cgaagaagaa cagacctaca aaggtggatt cgaggaagaa ggccatcaga
    26761 ccttcgattg ccagcggcgc accgaagata tcccctacat agtgggaata gtaagaccag
    26821 ttagtcccga actggaactc catggtcaga ccggtagcca cacccagagc gaagttgata
    26881 ccaaacaact tgccccagaa cttggtcata tctttataaa tctgtttgcc ggagaggacg
    26941 tagaccgttt ccataatggc caggaggaac gccataccga gcgtcagtgg cacaaaaagg
    27001 aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat atctaacatc
    27061 atgactcctt gctcatcgca tgaagactcc gagagtgaac cccgttagaa agggtcacac
    27121 gcatgcccca atagaaattt gtttgaacac tttcatcata ccatgtgaat gcacacaaaa
    27181 acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc aaattgatcc cacgtatata
    27241 ttacgccgca aaatccttac aataaacagg tttttgttga agcaaatttg catttttcga
    27301 cggtgatcaa tttatagcta aattgccgcc tttcagccaa tttgatcgag aacaatttat
    27361 ctctttttga tgcccatttc caagacttat acattgata
//
LOCUS       DAZOYF010000040        27053 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZOYF010000040 DAZOYF010000000
VERSION     DAZOYF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.040"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..176)
                     /locus_tag="ACHUOF_001613"
     CDS             complement(<1..176)
                     /locus_tag="ACHUOF_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR0610838.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="ACHUOF_001614"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="ACHUOF_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0610839.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="ACHUOF_001615"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="ACHUOF_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR0610840.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="ACHUOF_001616"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="ACHUOF_001616"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR0610841.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="ACHUOF_001617"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="ACHUOF_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR0610842.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="ACHUOF_001618"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="ACHUOF_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR0610843.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="ACHUOF_001619"
     CDS             complement(5180..5422)
                     /locus_tag="ACHUOF_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR0610844.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="ACHUOF_001620"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="ACHUOF_001620"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR0610845.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="ACHUOF_001621"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="ACHUOF_001621"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR0610846.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAKSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="ACHUOF_001622"
     CDS             complement(8339..9313)
                     /locus_tag="ACHUOF_001622"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR0610847.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="ACHUOF_001623"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="ACHUOF_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR0610848.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="ACHUOF_001624"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="ACHUOF_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR0610849.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="ACHUOF_001625"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="ACHUOF_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR0610850.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="ACHUOF_001626"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="ACHUOF_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR0610851.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="ACHUOF_001627"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="ACHUOF_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR0610852.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="ACHUOF_001628"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="ACHUOF_001628"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR0610853.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="ACHUOF_001629"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="ACHUOF_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR0610854.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="ACHUOF_001630"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="ACHUOF_001630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR0610855.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="ACHUOF_001631"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="ACHUOF_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR0610856.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /gene="aaeX"
                     /locus_tag="ACHUOF_001632"
     CDS             23180..23383
                     /gene="aaeX"
                     /locus_tag="ACHUOF_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR0610857.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="ACHUOF_001633"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="ACHUOF_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR0610858.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="ACHUOF_001634"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="ACHUOF_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR0610859.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTHFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="ACHUOF_001635"
     CDS             26388..26660
                     /locus_tag="ACHUOF_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR0610860.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="ACHUOF_001636"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="ACHUOF_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR0610861.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6448 a         6789 c         7228 g         6588 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaactggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatctg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagagt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgggcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattt agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctca ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ctggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgcattt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag gacaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca cta
//
LOCUS       DAZOYF010000041        26902 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZOYF010000041 DAZOYF010000000
VERSION     DAZOYF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.041"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(1..441)
                     /locus_tag="ACHUOF_001637"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="ACHUOF_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="ACHUOF_001638"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="ACHUOF_001638"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR0610862.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="ACHUOF_001639"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="ACHUOF_001639"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR0610863.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /gene="araG"
                     /locus_tag="ACHUOF_001640"
     CDS             complement(2384..3898)
                     /gene="araG"
                     /locus_tag="ACHUOF_001640"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR0610864.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /gene="araF"
                     /locus_tag="ACHUOF_001641"
                     /pseudo
     CDS             complement(3968..4957)
                     /gene="araF"
                     /locus_tag="ACHUOF_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5754..6257
                     /locus_tag="ACHUOF_001642"
     CDS             5754..6257
                     /locus_tag="ACHUOF_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR0610865.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /gene="yecJ"
                     /locus_tag="ACHUOF_001643"
     CDS             complement(6337..6588)
                     /gene="yecJ"
                     /locus_tag="ACHUOF_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR0610866.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="ACHUOF_001644"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="ACHUOF_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR0610867.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="ACHUOF_001645"
     CDS             7052..7375
                     /locus_tag="ACHUOF_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR0610868.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="ACHUOF_001646"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="ACHUOF_001646"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR0610869.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="ACHUOF_001647"
     CDS             complement(8080..8319)
                     /locus_tag="ACHUOF_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR0610870.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="ACHUOF_001648"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="ACHUOF_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR0610871.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="ACHUOF_001649"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="ACHUOF_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR0610872.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="ACHUOF_001650"
     tRNA            complement(10643..10729)
                     /locus_tag="ACHUOF_001650"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="ACHUOF_001651"
     tRNA            complement(10742..10815)
                     /locus_tag="ACHUOF_001651"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="ACHUOF_001652"
     tRNA            complement(10870..10945)
                     /locus_tag="ACHUOF_001652"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="ACHUOF_001653"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="ACHUOF_001653"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR0610873.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="ACHUOF_001654"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="ACHUOF_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR0610874.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="ACHUOF_001655"
     CDS             complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="ACHUOF_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR0610875.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14329..14400
                     /locus_tag="ACHUOF_001656"
                     /pseudo
     CDS             14329..14400
                     /locus_tag="ACHUOF_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            14483..14659
                     /gene="yecU"
                     /locus_tag="ACHUOF_001657"
     CDS             14483..14659
                     /gene="yecU"
                     /locus_tag="ACHUOF_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR0610876.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14646..14870
                     /gene="yecF"
                     /locus_tag="ACHUOF_001658"
     CDS             14646..14870
                     /gene="yecF"
                     /locus_tag="ACHUOF_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR0610877.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="ACHUOF_001659"
                     /pseudo
     CDS             complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="ACHUOF_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15753..16505)
                     /gene="tcyN"
                     /locus_tag="ACHUOF_001660"
     CDS             complement(15753..16505)
                     /gene="tcyN"
                     /locus_tag="ACHUOF_001660"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR0610878.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="ACHUOF_001661"
     CDS             complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="ACHUOF_001661"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR0610879.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="ACHUOF_001662"
     CDS             complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="ACHUOF_001662"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR0610880.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="ACHUOF_001663"
     CDS             complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="ACHUOF_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0610881.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="ACHUOF_001664"
     CDS             complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="ACHUOF_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR0610882.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="ACHUOF_001665"
                     /pseudo
     CDS             complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="ACHUOF_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="ACHUOF_001666"
     CDS             complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="ACHUOF_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR0610883.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22563..23969
                     /gene="fliD"
                     /locus_tag="ACHUOF_001667"
     CDS             22563..23969
                     /gene="fliD"
                     /locus_tag="ACHUOF_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR0610884.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23994..24404
                     /gene="fliS"
                     /locus_tag="ACHUOF_001668"
     CDS             23994..24404
                     /gene="fliS"
                     /locus_tag="ACHUOF_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR0610885.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24404..24769
                     /gene="fliT"
                     /locus_tag="ACHUOF_001669"
     CDS             24404..24769
                     /gene="fliT"
                     /locus_tag="ACHUOF_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR0610886.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24847..26334
                     /gene="amyA"
                     /locus_tag="ACHUOF_001670"
     CDS             24847..26334
                     /gene="amyA"
                     /locus_tag="ACHUOF_001670"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR0610887.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26368..26781)
                     /gene="yedD"
                     /locus_tag="ACHUOF_001671"
     CDS             complement(26368..26781)
                     /gene="yedD"
                     /locus_tag="ACHUOF_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR0610888.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
BASE COUNT         6796 a         6944 c         6321 g         6841 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
    14221 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
    14281 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
    14341 gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat
    14401 tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
    14461 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt
    14521 ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat
    14581 ggtatactcc gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa
    14641 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
    14701 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
    14761 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
    14821 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
    14881 gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct
    14941 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15001 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15061 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15121 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15181 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15241 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15301 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15361 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15421 gagcctgaag ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca
    15481 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    15541 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    15601 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    15661 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    15721 acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac
    15781 tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    15841 atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    15901 tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact
    15961 tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga
    16021 cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
    16081 ggatagctgg tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc
    16141 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16201 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16261 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16321 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt
    16381 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16441 ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca
    16501 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    16561 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    16621 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    16681 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    16741 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    16801 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    16861 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
    16921 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    16981 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17041 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17101 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17161 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17221 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17281 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17341 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17401 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17461 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    17521 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
    17581 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
    17641 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
    17701 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg
    17761 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa
    17821 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    17881 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
    17941 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18001 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt
    18061 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18121 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
    18181 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18241 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18301 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
    18361 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18421 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18481 agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg
    18541 tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag
    18601 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    18661 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
    18721 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
    18781 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    18841 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    18901 tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc
    18961 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19021 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19081 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19141 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19201 tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg
    19261 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19321 tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga
    19381 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta
    19441 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19501 cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc
    19561 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    19621 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
    19681 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    19741 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatgtcc
    19801 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    19861 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    19921 tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    19981 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca
    20041 tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata
    20101 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20161 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20221 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta
    20281 aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc
    20341 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20401 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20461 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    20521 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    20581 gttacgcaaa aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag
    20641 acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca
    20701 cggagttacc ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat
    20761 agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt
    20821 tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga
    20881 tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt
    20941 cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag
    21001 tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt
    21061 tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc
    21121 tataacctgc agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag
    21181 ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat
    21241 taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg
    21301 ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc
    21361 gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc
    21421 tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa
    21481 ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt
    21541 atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt
    21601 tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt
    21661 tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag
    21721 ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa
    21781 acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc
    21841 cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt
    21901 tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct
    21961 gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac
    22021 ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag
    22081 aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac
    22141 ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag
    22201 acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga
    22261 tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag
    22321 tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt
    22381 ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga
    22441 aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt
    22501 gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc
    22561 gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg
    22621 atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt
    22681 ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg
    22741 ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca
    22801 ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca
    22861 cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg
    22921 cgatcgccac cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta
    22981 ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg
    23041 caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag
    23101 tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg
    23161 cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg
    23221 tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca
    23281 ctatcagcga cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc
    23341 agacgctaac catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga
    23401 atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg
    23461 cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga
    23521 cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa
    23581 cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg
    23641 acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg
    23701 atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga
    23761 caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta
    23821 aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat
    23881 ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc
    23941 agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg
    24001 cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca
    24061 gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc
    24121 gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca
    24181 acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc
    24241 aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca
    24301 acgatgtctc agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga
    24361 aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca
    24421 tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac
    24481 ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga
    24541 gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc
    24601 gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat
    24661 ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta
    24721 tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag
    24781 ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt
    24841 cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt
    24901 aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg
    24961 gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc
    25021 tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt
    25081 gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg
    25141 ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag
    25201 cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg
    25261 acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa
    25321 tgttttagcg gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt
    25381 aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat
    25441 tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac
    25501 tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa
    25561 catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag
    25621 ccgctgttta ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt
    25681 gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa
    25741 gcatcgcgca tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa
    25801 gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc
    25861 ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg
    25921 gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc
    25981 ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta
    26041 attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac
    26101 tgcattgcct ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg
    26161 aacggagatg atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg
    26221 cgtgattttc tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc
    26281 ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat
    26341 ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc
    26401 cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta
    26461 ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt
    26521 gagatcatca gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg
    26581 gcaatccaag gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac
    26641 cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt
    26701 tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc
    26761 tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt
    26821 aaagctctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt
    26881 ttgtgaatta ttaccccgaa aa
//
LOCUS       DAZOYF010000042        26184 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZOYF010000042 DAZOYF010000000
VERSION     DAZOYF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.042"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..213)
                     /locus_tag="ACHUOF_001672"
     CDS             complement(<1..213)
                     /locus_tag="ACHUOF_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFR0610889.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="ACHUOF_001673"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="ACHUOF_001673"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR0610890.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="ACHUOF_001674"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="ACHUOF_001674"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR0610891.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="ACHUOF_001675"
     CDS             2894..3634
                     /locus_tag="ACHUOF_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0610892.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="ACHUOF_001676"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="ACHUOF_001676"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR0610893.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="ACHUOF_001677"
     CDS             complement(4192..4794)
                     /locus_tag="ACHUOF_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR0610894.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4864..4929
                     /gene="yobI"
                     /locus_tag="ACHUOF_001678"
     CDS             4864..4929
                     /gene="yobI"
                     /locus_tag="ACHUOF_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFR0610895.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5068..5679
                     /gene="ruvA"
                     /locus_tag="ACHUOF_001679"
     CDS             5068..5679
                     /gene="ruvA"
                     /locus_tag="ACHUOF_001679"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR0610896.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5688..6698
                     /gene="ruvB"
                     /locus_tag="ACHUOF_001680"
     CDS             5688..6698
                     /gene="ruvB"
                     /locus_tag="ACHUOF_001680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR0610897.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="ACHUOF_001681"
     CDS             complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="ACHUOF_001681"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR0610898.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="ACHUOF_001682"
     CDS             complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="ACHUOF_001682"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR0610899.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8461..9393
                     /gene="znuA"
                     /locus_tag="ACHUOF_001683"
     CDS             8461..9393
                     /gene="znuA"
                     /locus_tag="ACHUOF_001683"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR0610900.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9409..10731
                     /gene="mepM"
                     /locus_tag="ACHUOF_001684"
     CDS             9409..10731
                     /gene="mepM"
                     /locus_tag="ACHUOF_001684"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR0610901.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10851..11822
                     /gene="lpxM"
                     /locus_tag="ACHUOF_001685"
                     /gene_synonym="msbB"
     CDS             10851..11822
                     /gene="lpxM"
                     /locus_tag="ACHUOF_001685"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0610902.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="ACHUOF_001686"
     CDS             complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="ACHUOF_001686"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR0610903.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="ACHUOF_001687"
                     /gene_synonym="yebK"
     CDS             complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="ACHUOF_001687"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR0610904.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14729..16204
                     /gene="zwf"
                     /locus_tag="ACHUOF_001688"
     CDS             14729..16204
                     /gene="zwf"
                     /locus_tag="ACHUOF_001688"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR0610905.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16439..18250
                     /gene="edd"
                     /locus_tag="ACHUOF_001689"
     CDS             16439..18250
                     /gene="edd"
                     /locus_tag="ACHUOF_001689"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR0610906.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18287..18928
                     /gene="kdgA"
                     /locus_tag="ACHUOF_001690"
                     /gene_synonym="eda"
     CDS             18287..18928
                     /gene="kdgA"
                     /locus_tag="ACHUOF_001690"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR0610907.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18984..20162)
                     /gene="purT"
                     /locus_tag="ACHUOF_001691"
     CDS             complement(18984..20162)
                     /gene="purT"
                     /locus_tag="ACHUOF_001691"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR0610908.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20296..20586
                     /gene="yebG"
                     /locus_tag="ACHUOF_001692"
                     /pseudo
     CDS             20296..20586
                     /gene="yebG"
                     /locus_tag="ACHUOF_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20653..21009
                     /gene="yebF"
                     /locus_tag="ACHUOF_001693"
     CDS             20653..21009
                     /gene="yebF"
                     /locus_tag="ACHUOF_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR0610909.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21336..21995
                     /locus_tag="ACHUOF_001694"
     CDS             21336..21995
                     /locus_tag="ACHUOF_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR0610910.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22205..24265
                     /gene="ptrB"
                     /locus_tag="ACHUOF_001695"
     CDS             22205..24265
                     /gene="ptrB"
                     /locus_tag="ACHUOF_001695"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR0610911.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="ACHUOF_001696"
     CDS             complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="ACHUOF_001696"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR0610912.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24948..25604)
                     /gene="yobB"
                     /locus_tag="ACHUOF_001697"
                     /pseudo
     CDS             complement(24948..25604)
                     /gene="yobB"
                     /locus_tag="ACHUOF_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
     gene            complement(25706..25936)
                     /gene="holE"
                     /locus_tag="ACHUOF_001698"
     CDS             complement(25706..25936)
                     /gene="holE"
                     /locus_tag="ACHUOF_001698"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR0610913.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26075..>26184
                     /locus_tag="ACHUOF_001699"
     CDS             26075..>26184
                     /locus_tag="ACHUOF_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610914.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAA"
BASE COUNT         6652 a         6485 c         6818 g         6229 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc
     4861 gcaatgtata tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt
     4921 ccaatataac ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg
     4981 caagttttct tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat
     5041 ctttgattca ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa
     5101 aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg
     5161 atgacctgtt tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt
     5221 gtggtgcgtg aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg
     5281 ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc
     5341 ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg
     5401 aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga
     5461 tttaaaggtt tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct
     5521 gctagcccgg cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc
     5581 tataaaccac aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt
     5641 gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag
     5701 accgtctgat ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc
     5761 ctaaattact ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca
     5821 tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg
     5881 ggttgggtaa aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca
     5941 cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg
     6001 aaccgcatga cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag
     6061 tgctgtaccc ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg
     6121 cacgctccat taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag
     6181 gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc
     6241 aggtgccgga tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga
     6301 gtgatgacgg tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc
     6361 gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag
     6421 atatcgctgc tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg
     6481 accgcaaatt gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata
     6541 acctggcggc agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt
     6601 tgattcagca aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt
     6661 ggaatcactt tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg
     6721 catccgacac tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga
     6781 gatagcgaac cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga
     6841 tagattagct ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca
     6901 ccaccgacgg acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg
     6961 ccaccatccc caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac
     7021 gcgcagtagc tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca
     7081 tcgctacacc aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt
     7141 tcacaccatc aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt
     7201 gccagaacaa aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg
     7261 gcgtcactgc cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca
     7321 tcagactaac gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg
     7381 tgtcgatcgc cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg
     7441 taacggcaat caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa
     7501 gtaatgaggc atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg
     7561 aacccagcgg acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata
     7621 attcaatcat gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg
     7681 atgattatga tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga
     7741 aataaactcc ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt
     7801 caggcacagc acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa
     7861 aacgccacaa tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc
     7921 atttacatcc acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa
     7981 tgctcgcgct aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt
     8041 aatcaggtgc ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc
     8101 agggcgtaag cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt
     8161 ctgcggtaca tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt
     8221 taccagcccg agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa
     8281 agtcaaaatt tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc
     8341 aaaagaaacc gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca
     8401 gtagtcatga aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt
     8461 atgttacata aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca
     8521 acacaggccg cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct
     8581 gccattgctg atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat
     8641 gattattcac tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg
     8701 gttggcccgg agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag
     8761 caggtaacga ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc
     8821 gatgatgatg atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc
     8881 aacatgcatc tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga
     8941 aaattagtgg aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt
     9001 gaggcacaat tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg
     9061 aaaggttatt tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca
     9121 ccgcttggtc attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa
     9181 ataagaacac agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg
     9241 ccagcggtcg ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc
     9301 ctggggacga atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc
     9361 aaccagtatg cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata
     9421 gcccgctctg tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg
     9481 tcgctcaccg ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat
     9541 gccacgccaa ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct
     9601 gaagccagtg agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa
     9661 ctggatgaca aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat
     9721 acgctaagca gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct
     9781 gctgccgaca aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc
     9841 gcggacggcg aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat
     9901 gaccgtactg ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt
     9961 aacaatctgc tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt
    10021 ttaaccagcg ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc
    10081 aaactgaaaa aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa
    10141 cgtgagcaaa gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca
    10201 atccgcgctg aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc
    10261 ttgcgattcc cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact
    10321 aatccggtga caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt
    10381 acgccagtgc tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca
    10441 ggttattatg tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc
    10501 aagattctgg tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt
    10561 aataccggac gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc
    10621 gtaaacccgc tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc
    10681 gaattcctgg cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc
    10741 attcgcagcc ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc
    10801 cgctacacta tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga
    10861 aaaaaaataa tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact
    10921 ggggagcatg gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc
    10981 gtgatcccat tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc
    11041 gtcgtgcgtt aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag
    11101 cgattgttga tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg
    11161 caatacgcgg gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg
    11221 aagagatgcg gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg
    11281 atattcctgc catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc
    11341 agggcaaccc ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc
    11401 tgcatgcgag aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg
    11461 gatattattt acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg
    11521 ccacctataa agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg
    11581 ttgtaccgct gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc
    11641 caccgatgga tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag
    11701 aagtcgagat ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga
    11761 aaactcgcaa accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat
    11821 aaaaaagcct ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa
    11881 tattcgaaat ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat
    11941 ccggcaacgt acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact
    12001 catcacgtcg ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg
    12061 cagcagatta accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac
    12121 cggcgtaacg ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc
    12181 gaaaattggt agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt
    12241 catggtaatg atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc
    12301 aatagcttct tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct
    12361 cgggattttt tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata
    12421 ctgcccagcg gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt
    12481 tgctacgtcc atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat
    12541 ctgggtcgcc gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg
    12601 aatgccgacc agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac
    12661 cacgtcagag gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg
    12721 ttcaaccttg gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata
    12781 gttcagatct tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc
    12841 cgcagtctta atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc
    12901 aagtttgttg atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac
    12961 tttcatgccc tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat
    13021 gtcaccaggc acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc
    13081 gccttcacct ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac
    13141 tttgccttct ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc
    13201 cacatgacgc cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc
    13261 ttcaggcgag ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt
    13321 ttcaagatta ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg
    13381 aagccttttg gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga
    13441 aatccgaatg aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag
    13501 attaggacag tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa
    13561 aacgcgattc cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc
    13621 gtcgcaaagt aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa
    13681 ccatgggcat ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt
    13741 cccgggcgag cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg
    13801 ccagctgcgc cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca
    13861 cgtctccgtc gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca
    13921 ccaccggaac attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag
    13981 agcctaatcc gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga
    14041 tggcagattt atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata
    14101 ttttccccgt gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag
    14161 tgccattcgc cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca
    14221 tgctgcgaca gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag
    14281 caatactcga atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct
    14341 ctgatttgct caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa
    14401 gagactcacg ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga
    14461 tattacgcct gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca
    14521 taacatgatc agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg
    14581 ccgcgctttg cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca
    14641 agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta
    14701 ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga
    14761 cctggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta
    14821 tcaactggaa aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc
    14881 tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa
    14941 agaaaccatt gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct
    15001 cgatgtcaat gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg
    15061 tatcactatt aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct
    15121 tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac
    15181 gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca
    15241 ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg
    15301 ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat
    15361 taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca
    15421 gatgcgtgac atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc
    15481 gccatctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct
    15541 gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg
    15601 cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag
    15661 caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt
    15721 gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta
    15781 tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa
    15841 actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc
    15901 tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac
    15961 ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg
    16021 tattcaggca ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc
    16081 cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg
    16141 gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga
    16201 gtaatatcag cgcttatcct ttatgggtat tttaccggta acatgatctt gcacagattg
    16261 tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta
    16321 cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc
    16381 tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat
    16441 gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc
    16501 tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc
    16561 atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa
    16621 aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca
    16681 ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt
    16741 tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg
    16801 aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc
    16861 ccataacatg tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct
    16921 gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat
    16981 ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa
    17041 agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg
    17101 tactttctac ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt
    17161 gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc
    17221 tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat
    17281 gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac
    17341 taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga
    17401 tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc
    17461 ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct
    17521 caaagcaggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta
    17581 tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc
    17641 actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa
    17701 agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa
    17761 ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt
    17821 tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc
    17881 gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg
    17941 tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc
    18001 agctatccac gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg
    18061 ggacatcatt cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga
    18121 actggctgct cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg
    18181 tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat
    18241 cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa
    18301 aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa
    18361 actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga
    18421 agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc
    18481 tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga
    18541 agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac
    18601 cgaagggact attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat
    18661 ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc
    18721 cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc
    18781 tccggctaac taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg
    18841 gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg
    18901 tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt
    18961 tttgtagcgt ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc
    19021 gcgcgttcaa tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg
    19081 ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc
    19141 tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct
    19201 gcaggaccat actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg
    19261 gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg
    19321 gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca
    19381 taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc
    19441 gggctcattt gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct
    19501 actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag
    19561 tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga
    19621 gcgtattccc atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg
    19681 gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg
    19741 aaaaggcttt tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc
    19801 gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca
    19861 acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc
    19921 ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc
    19981 atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg
    20041 acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca
    20101 ccggagccta ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc
    20161 ataacgaata tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc
    20221 tttattgctg atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat
    20281 tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag
    20341 aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg
    20401 gatctgcttg acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa
    20461 gccctttcgc tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc
    20521 aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg
    20581 gcctgaagca acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct
    20641 atggagaaaa acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc
    20701 gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa
    20761 ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg
    20821 gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt
    20881 ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa
    20941 agtgccgata ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag
    21001 cggcgttaat ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg
    21061 tccgtcccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc
    21121 aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat
    21181 cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc
    21241 cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg
    21301 ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc
    21361 aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca
    21421 aactgttagt gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag
    21481 cacgtaaact tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag
    21541 cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac
    21601 cgcagtttgg cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc
    21661 tggtctttgc cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc
    21721 agcaattgcg tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg
    21781 aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg
    21841 aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc
    21901 tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg
    21961 atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt
    22021 tgactatttc gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct
    22081 gcataacatt cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt
    22141 cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa
    22201 taacatgcta ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg
    22261 catcgataat tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta
    22321 cctgcaacaa gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga
    22381 tcgcatctta aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat
    22441 aaaaaatggc taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca
    22501 gcgtcaatcg gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa
    22561 gcgcgcagct catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac
    22621 cattatggcg ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa
    22681 tctggaaaca ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg
    22741 ggcaaatgac tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta
    22801 tcaggtctgg cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga
    22861 aaaagacgat acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat
    22921 tcatctggcc agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc
    22981 cgagccgttt gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca
    23041 gcatcgtttt tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg
    23101 tatgcgtgat gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga
    23161 agggtttacg ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag
    23221 tttgcgccaa attaaccgca agacccggga agtcattggc attgcctttg atgatccggc
    23281 ctatgtgacc tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta
    23341 ttcttccatg accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg
    23401 tgtattaaaa caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct
    23461 gtggatagtc gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca
    23521 ttttcgcaaa ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat
    23581 tgatgccgat ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat
    23641 tgtccatgtt cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct
    23701 gaagaagaaa aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg
    23761 ctatggctct ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg
    23821 cgttgcaatt aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt
    23881 tgatgttgta acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga
    23941 gtggggaaat ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga
    24001 caacgtcacc gcacaggctt atccgcattt actggtaacg accggtttac acgattctca
    24061 ggtgcaatat tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga
    24121 ccatctttta ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt
    24181 taaatcgtac gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac
    24241 attacccgct acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag
    24301 ctccgggctc atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc
    24361 ggcaacgtcg gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga
    24421 cggacgtccg gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc
    24481 gataagcaac gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag
    24541 cggtgtctgt acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc
    24601 tggccacaaa cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag
    24661 tacgcggcgg tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg
    24721 gatcacatct tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc
    24781 catcgcttgt ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat
    24841 ttttccgtca atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt
    24901 ttctgtatcg ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga
    24961 atgatatcgc cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga
    25021 tcggcgcgaa cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt
    25081 gccatcagca cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc
    25141 agcaggtcgg gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata
    25201 tccatacctt gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat
    25261 gcaccatgac tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt
    25321 ccacgaataa agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag
    25381 gtcgttgcgg cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca
    25441 cgtcgcgaat aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt
    25501 tgcctggcgg cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt
    25561 ttacgaggct catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca
    25621 aaatgactgc aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt
    25681 aagaaagtgt aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca
    25741 gattgaccga agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt
    25801 ctggctgttc acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag
    25861 ccaccccggc cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag
    25921 ccagattctt cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga
    25981 gcctggatgg caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc
    26041 tgtaaaaaag ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg
    26101 ctacgcgctg gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca
    26161 tttaacgcat cagtatcctg cggc
//
LOCUS       DAZOYF010000043        26121 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZOYF010000043 DAZOYF010000000
VERSION     DAZOYF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.043"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            376..815
                     /gene="ycgY"
                     /locus_tag="ACHUOF_001700"
                     /pseudo
     CDS             376..815
                     /gene="ycgY"
                     /locus_tag="ACHUOF_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
     gene            complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHUOF_001701"
     CDS             complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHUOF_001701"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR0610915.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHUOF_001702"
     CDS             complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHUOF_001702"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFR0610916.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHUOF_001703"
     CDS             complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHUOF_001703"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFR0610917.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHUOF_001704"
     CDS             complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHUOF_001704"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFR0610918.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHUOF_001705"
     CDS             6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHUOF_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFR0610919.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(8300..11167)
                     /locus_tag="ACHUOF_001706"
     CDS             complement(8300..11167)
                     /locus_tag="ACHUOF_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0610920.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(12011..13102)
                     /gene="ychF"
                     /locus_tag="ACHUOF_001707"
     CDS             complement(12011..13102)
                     /gene="ychF"
                     /locus_tag="ACHUOF_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR0610921.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(13219..13803)
                     /gene="pth"
                     /locus_tag="ACHUOF_001708"
     CDS             complement(13219..13803)
                     /gene="pth"
                     /locus_tag="ACHUOF_001708"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR0610922.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            14081..14359
                     /gene="ychH"
                     /locus_tag="ACHUOF_001709"
     CDS             14081..14359
                     /gene="ychH"
                     /locus_tag="ACHUOF_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR0610923.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(14414..16093)
                     /gene="dauA"
                     /locus_tag="ACHUOF_001710"
     CDS             complement(14414..16093)
                     /gene="dauA"
                     /locus_tag="ACHUOF_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR0610924.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(16218..17165)
                     /gene="prs"
                     /locus_tag="ACHUOF_001711"
     CDS             complement(16218..17165)
                     /gene="prs"
                     /locus_tag="ACHUOF_001711"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR0610925.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(17316..18167)
                     /gene="ispE"
                     /locus_tag="ACHUOF_001712"
     CDS             complement(17316..18167)
                     /gene="ispE"
                     /locus_tag="ACHUOF_001712"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR0610926.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(18167..18790)
                     /gene="lolB"
                     /locus_tag="ACHUOF_001713"
     CDS             complement(18167..18790)
                     /gene="lolB"
                     /locus_tag="ACHUOF_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR0610927.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            19004..20260
                     /gene="hemA"
                     /locus_tag="ACHUOF_001714"
     CDS             19004..20260
                     /gene="hemA"
                     /locus_tag="ACHUOF_001714"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR0610928.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            20302..21384
                     /gene="prfA"
                     /locus_tag="ACHUOF_001715"
     CDS             20302..21384
                     /gene="prfA"
                     /locus_tag="ACHUOF_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR0610929.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            21384..22217
                     /gene="prmC"
                     /locus_tag="ACHUOF_001716"
     CDS             21384..22217
                     /gene="prmC"
                     /locus_tag="ACHUOF_001716"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0610930.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            22214..22606
                     /gene="sirB2"
                     /locus_tag="ACHUOF_001717"
     CDS             22214..22606
                     /gene="sirB2"
                     /locus_tag="ACHUOF_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR0610931.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            22610..23419
                     /gene="sirB1"
                     /locus_tag="ACHUOF_001718"
     CDS             22610..23419
                     /gene="sirB1"
                     /locus_tag="ACHUOF_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR0610932.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            23455..24309
                     /gene="kdsA"
                     /locus_tag="ACHUOF_001719"
     CDS             23455..24309
                     /gene="kdsA"
                     /locus_tag="ACHUOF_001719"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR0610933.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24458..24565)
                     /locus_tag="ACHUOF_001720"
     CDS             complement(24458..24565)
                     /locus_tag="ACHUOF_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0610934.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(24970..26070)
                     /gene="chaA"
                     /locus_tag="ACHUOF_001721"
     CDS             complement(24970..26070)
                     /gene="chaA"
                     /locus_tag="ACHUOF_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR0610935.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         6493 a         6487 c         6603 g         6538 t
ORIGIN      
        1 cattttatta atgcatttct cattcgggtg aaatttccgc tacctggacg cataaaaccg
       61 gaactctcgc caaaagtcgg gcactccgaa ggaattgacg cgttgcgctg ctctgtaacg
      121 gcatttaccg caagccattt atttcgccag cgacaatccg cagagaacca aacgcatcag
      181 gaaacgactg aacacaataa atactgtatt gatcaacata ttatactggc acctccagca
      241 cgcagtgagg atacactgta tcatcagcga atcttatcac tgcggagctg gttgaacgac
      301 tttctgagcg aaataagtcg gttcccggta acaacaacag aatccttttt taattattgt
      361 ttcgttgttt ttggtgtgat tcagaaaata aatattttag acagtagcca gtcaggcgtt
      421 aaaaatgggg cggatagtga ggcggttttg gctgcgctta ttgaacattt agaattgatc
      481 aatccttctg gcagattaag ccaaaatacc cgttcggcaa tgcttcagtt acgcgaagag
      541 gaatggtctg aattcttttt ttggttgcta aattctctgg agtgtctgga ttatgtgatt
      601 attaatttaa ctccagaaag caaaaaaacg ttgatgagtg aacaccgtaa caatattcgg
      661 tagcaattga tgctttatat agccaacgtc ggcgtaaatc gccgggtgat gaaagtgaaa
      721 cacttacccg tagaaatgac gctatcttcg gcaaccacgt atggcaaaca tttgcgcaat
      781 acttcccgcc aggacttgaa aaaccgtctg tgtagaaaaa accatagata tggtgttatc
      841 tgccgacaac gccatccgga gaagccgaat gacgttgtcg gagtaagcgc tggttaaggt
      901 gtgggttgtg cctctttggt tgagggttgc gtcgttgctg acttaacggt cggatgcgtc
      961 gccggaacat tgtcacacgg ttgctctttc gggcagatca aatccagcat tttcagcgtc
     1021 acgccattgg tccagccaaa gccatcctgt aatggatatt cgccaccgcc gccccccgtt
     1081 ccggtggtgc tgacatcata tttttccacc agctttttct cccggtcata ggtgtgctga
     1141 acattggtca ggaagtgcca gctaatgtcc atcgccacct ctttttgccc gtagttttgt
     1201 aatccttctg tcgcgaccca ctgtaacggt gcccagccat ttggcgcatc ccattgttgc
     1261 ccacttttca ccgacgtggt gttcaggccg ccgggttgca gcagatgtgt tttcgtcgcc
     1321 gtcgccattt tgttggcgcg atctttcgct gccgcattga cgtacagcgg gaacagggcg
     1381 gccgcggtta actgattgcg cactttatga cttttcaggt cgtaatcggc ataccagcct
     1441 tgttgatcgt tccacaggta tttttcgatc cctttttgac gggcattcgc cagcgtttcg
     1501 tactggtttg ccatcgcgtt atctcctgca gctttgctgg cgcgggcgag gattttttcc
     1561 attttaaaca tcaggctgtt cagatcgacc ggtacgatgc tggtggtgcg taaggtattt
     1621 aactgctgcg ggttgtccat ccagcgcgag ctgaaatccc agccagacgc agcggcagag
     1681 cgcaggtcgc ggtaaatttc agtggcaggt cgattcggat tgcttttggc ggtggcaata
     1741 tcttccaccc atgactctgg tcgtggcgta tcgcgatcgt cccagtagcg gttgagaagg
     1801 gtactatcct gaagttttac aacgcgtttt tcctgttgtc cggcttgcag gttttcaaca
     1861 ccgtccatcc agtaagcata ttctttttgc atttgcggca ggtattgctt caacgcggca
     1921 tcgccttcat gctgcgccag taactctacc atcagggcaa agaagggcgg ttgcgagcgg
     1981 cttaaatagt aactgcggtt gccgttggga atatgaccgt aagtgtttat ttcatgagca
     2041 aaattggcca ccatatccgc gattttatcc cagtgaccac tttcggcaag tcctaacatg
     2101 gtgaagtaat tgtcccagta atatacctcg cgaaaacgtc cgcccggcac gacataaggt
     2161 tccggcagcg gtaacagaga atcccatttt tcggtgtttt cggtagaacg cgttaatacc
     2221 ggccaaagtc cgtcaatatg ttcgcgcagt gactgcccct ctggcggaac atatttctcg
     2281 ccttctttcg gcagggtgaa attgacgtta acgaaatggc gcagatcaaa tccgctctgg
     2341 ttttgctgca tccgataatc agcaaggatc atcagcggat cgctgttagg cacggcatcg
     2401 gcaaaggttt tttggtccgg aaaaagtttg acgttttgca catcattaaa cagcggccct
     2461 aataaaatat caggcggctg tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca
     2521 gcgaaacaca aaaagataca ggctggaatt aacgccattt tttgcgggcg agaaggtgcg
     2581 ggggatttca tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag
     2641 aaaaccttag ttcaggatcg cactagctgc atgatcgaaa tctcattctg cgaacggtca
     2701 ggcattttag aaaagggaat taaagggata aataacggcg aaatagagtg atcaaataac
     2761 gtctgtttat tagccagcca gcgacatttg gttcgcgatt agcgaagaaa aatcatcata
     2821 aattggtaaa aaatataaca ggtagttaaa aaacatcagt gctgggtaaa ttgccggatg
     2881 acatcagaac gatgccatcc gaacggtggc ttaaccctga cggttgaaac gttgcgtttt
     2941 aacgtccagc gttagcgttt cttctggttg tatcgcatac agtttctcac cctgctggca
     3001 aatccagcca atccccagtt cacgggcgat tagggcgctg tgggataccg gacttccggc
     3061 gctaaggcag atacctttta caaccgccgg atccagttgc agtactgtgg aaggataaat
     3121 gttctccgcc agtagaatag ttggcgagtt aaactgcggg agttcttctt tcgtttgggt
     3181 caggtggacc agggtgcgat gcagaagatc gtccacatca atatagcgag cttgtagata
     3241 ttcatcatcc agttgctggt attgctggct aagttcttta agaacttgct gccaggcata
     3301 ttctgccgtg caatgttcat gctgaaggag ttcgcttgcc gccgccagca gttccggatc
     3361 acctaacagt gtatggtgac cagaaaagat tgcggcaata tcgtcaagcc cgctggcttc
     3421 tgctttcgct gttaacgtca tcagatctaa taacgtgaag tcaatagcct ggcgtaatcg
     3481 atcttgttct tcttccgcgg tcagggttga ttttgcctgt accgtacata aaactggttg
     3541 ataataaaag gctttacccg aaacaggcag aacgggacgc agagtaggtg gagcgacttc
     3601 ctccgtttca ccaaagttat cttcagccag gtgacggaaa gcgatcagtg cctcttcagc
     3661 ttctggccct ttcgcaatca ggcgcagcgt atcgttatag cgaacttgta gtaacgcaat
     3721 ctggttaata ctctctggtg tgacgcattt gccgtttttt tccagcaaca tatcggcatt
     3781 aaatgtcgat aaggtataaa ccagccggga ggccggacgt acatgcaggc cgttacggtt
     3841 ttttatgacc accgccagag aacgggcttc ttcatcgtac ggaggacatg tgtcagagat
     3901 ttcagtgtcg gaggacggta aacccagttg ttcacgtttg gcttccagcg catgcatagc
     3961 gtcaaagata actttgtcga tatccgcccc cgaggccgcg ctgaccgttg ctgccagtgt
     4021 accttcgacc aacggcgcag cacacaaacg tacttttgcg gcgatctcgg gagccagcaa
     4081 ttccagcgca gtttcagcac tcaataatgc gctacccata tccatcatga ccagcacatg
     4141 gtcggcatca gcaacagatt cgatggcctc catcactttg acggcatcgg taccaatggg
     4201 attttgtgga tcgtcaattc ccgcggcaat ggcgatttta caactatcac tcattaacat
     4261 ctgacgggct aattcaccga caccttctcc cagtcggctg ctatgtgaaa ctatgaccag
     4321 gtttaccatc accaattcct tactcttttg cggctaacgc caacatttgc atcataaaca
     4381 tcaccgaggt cgcgccggga tcctggtgac caatactgcg ttcaccgaga taactggcgc
     4441 ggcctttgcg ggcttgcatc gtaatcgtac tttgtgcagc ggattcggcg atgctactgg
     4501 cagcttcgag cgccaccgga acagagagat tttgctcgct ggactgacgt aacgattcca
     4561 ccaccggcac ccacacatca cacatggttt tatcgccagg ttcggctttc ccgcgactga
     4621 ttacgccgtc cgcgccatcg cggaacatct gataaagctc ttccagtgtc aggctttgcc
     4681 gtgcctgggt cgcctgtgcg gcgcggataa agaaggtacc gaacagcgga ccactggcac
     4741 caccgacgct ggaaagcagc gtcataccgg tatttttgag aatgaaaccg atatctttat
     4801 ctgcgatagc agggagtttt tccaccactt tgctaaagcc tcggttcata tttagcccgt
     4861 ggtcagcgtc gccaatctcg cgatccagcc cggtaagata ctcgctctcg gtgttgaaaa
     4921 tatcgccaca acgagtgagc cagttaacaa tttgagttct gctcagtgac attgctttct
     4981 ccttatttac cccagttaag ggccggggtg tggaccgggg cgtcccagag agccagcgtt
     5041 tcgtcatcaa ctttcagtaa ggtgattgag aaaccggtca tatccagtga ggtgcagtac
     5101 gcgccaatta aattacgttc gatagtcaat cccgcttgct ggcaacgtgt ggtcaggcgg
     5161 ttatagacgc cgtacagctc agaaagcgga gttgcgccaa gattgttaac cagtgcaatc
     5221 acccgatcgc cagactggag cggttgtttg gtttgtggtt cttcctgcca gctgccttgt
     5281 tgataatccc agaaacgcaa agtgcgatgg tatgagccat gttccagcag ggtgtcgaac
     5341 atttcatcga cggtttgatc aagggaagag aaagggcggc ggtcaatacc tggctcacca
     5401 tgaatgccta cgccaaactc catctcatta tccgcaaggg taaaagaagg tttgcccgcg
     5461 gcaggaacgg tacaggcacc gagagcgata cctattgagt ggccttgatt attcaactta
     5521 cgccccagtt ccgcacaggc gtccagtgag tcgccacgct ccgccgctgc gcctacgagt
     5581 ttttcaatta atacggtgtt ggcaacgccg cgtcgcccgg cggtataaag actgtctttt
     5641 accgcaacgt cgtcatcaat gaccacagtg gtcactttta cgccgctatc gtgcagtaac
     5701 tcggtcgctg tttcaaagtt aagaatatcg ccggtgtaat ttttgataat caacagtaca
     5761 ccttcgccgc catcaatttg catggagcat tcaaagattt tatcgggcgt cggtgaggtg
     5821 aaaatttcgc ccggacaggc ccccgaaagc atcccctgac cgatataacc acagtgcatc
     5881 ggctcgtgtc cgctgccgcc acccgacagc agggcgactt ttcctgcaac aggggcatca
     5941 gctcgggtga catacaccgg atcctgatgc agtgtcagcg atggatgcgc tttcgccagt
     6001 cctgccagtt gttcgtccag tacgtcttgc acatcattga tcaatttttt cattattttg
     6061 ctccagcaat tacggtaggg catggatgat gttcaacgac acggcgacca ctgactgccg
     6121 atgaatccat tgtgcatcag gcaagggaaa agaaaattag cccaaattat gtttcatagt
     6181 gaaacatatg ctttaatgaa tgttccatat tgaaactttt acgtgtatta atacttaaaa
     6241 ttgcgagccg gaacaccttt tgtcataagg gatgcgggat atgagtggcg cttttaacaa
     6301 cgatggtcgg ggcatatctc ccttaattgc aacctcctgg gagcgatgca ataagctgat
     6361 gaaacgggag acatggaacg taccacatca ggcccagggc gtgacatttg cttctattta
     6421 tcggcgtaag aaagcgatgc tgacgctcgg gcaggctgcg ctggaagatg cctgggaata
     6481 tatggcaccg cgagagtgtg cgctgtttat cctcgatgaa accgcctgca ttctcagccg
     6541 taatggcgat ccgcaaacct tgcagcagct aagtgcactg ggattcaatg acggcacgta
     6601 ttgcgccgag ggaattattg gtacttgtgc gctatcgtta gcggctatct ctggtcaggc
     6661 cgtgaaaacg atggccgatc aacatttcaa acaggcactc tggaactggg ccttttgtgc
     6721 aacgccgttg tttgacagca agggccgatt gacgggaaca atagcgctgg cgtgtccggt
     6781 tgagcaaact accgcagctg atttgccgtt gacgttggca atcgcccgcg aggtcggtaa
     6841 tttactgctg acggacagtt tgctcgctga aactaaccgt catttaaatc aacttaatgc
     6901 cctgttagaa agtatggatg atggcgtgat tagctgggac gagcagggta atttgcaatt
     6961 tattaatgcc caggcggcgc gggtcttgcg ccttgacgcg acggcaagtc agggacgggc
     7021 aatcactgaa ctcttaacgt tacccgccgt attgcaacaa gcaataaaac tggcacatcc
     7081 gctcaaacac gtagaagcaa cctttgaaag ccagcaccag tttattgatg cggtgataac
     7141 ccttaaaccg ataatagaaa cgcagggaac cagctttatt ttgttgctcc atcctgtgga
     7201 acagatgcgg cagttaatga ccagtcaatt aggaaaagtc agccatacct tcgctcatat
     7261 gccacaggac gatccgcaaa cccgccgctt gattcatttt ggtcgccagg cggcgcgcag
     7321 tagctttcct gtcctgcttt gtggagaaga gggcgtgggc aaggcactgc taagtcaggc
     7381 aattcataat gaaagcgagc gtgctgcagg tccttatatc gccgtcaatt gtgagttata
     7441 tggtgatgct gcgctggcgg aagaatttat tggtggcgat cgcacggaca atgaaaatgg
     7501 ccgtctgagt cggctggaac tggcacacgg cggcacgctg tttcttgaaa agattgaata
     7561 tctggcggtg gagttacagt ctgctttgct gcaggttatc aagcaggggg ttatcacgcg
     7621 actggatgcg cggcgtttaa taccaattga tgtcaaagtg attgcaacaa cgaccgcgga
     7681 cctcgcaatg ctggtggaac aaaatcgttt tagtcgccag ctgtattacg cgctgcatgc
     7741 atttgaaatt accatcccgc ctctgcgtat gcggcgtggc agcattccgg cgctggtgaa
     7801 taacaaatta cgcagtcttg aaaaacgctt ctctacgcgg ctgaaaattg atgacgatgc
     7861 cctcgctcgc ctagtttctt gtgcatggcc aggcaacgat tttgaacttt acagcgtcat
     7921 cgagaatctt gctctgagta gtgataacgg gcgcattcgc gtcagtgatt tacctgaaca
     7981 tctgtttacc gagcaggcga cagatgatgt cagcgccacc cgcctttcca ccagtctgtc
     8041 atttgcggaa gttgaaaaag aggcaattat taacgcagcc caggtcacag gcggtcgcat
     8101 tcaggaaatg tcggctttac ttgggatcgg ccgcactacg ctgtggcgga aaatgaagca
     8161 acatggcatt gatgcagggc agtttaagcg ccgggtatga aagacagaaa cgatttctga
     8221 tacatcagag tgatctgtat ttcattccgg cgcacgctaa caattttcag catcgtttaa
     8281 gggcttgtct atcccgcact taaaagctga agcgatatcc tccgttgact tgcttctgat
     8341 caaagaggtt accctgcgtg taatccgctt cgagatagaa tgtgtgctgt ttgttatact
     8401 gtgcactgac tcccacgccg ttattccagc cattaccttt gaaactgtac ttctcccggg
     8461 agtcgttcaa cagatattcg gtatcccctg aaaactcgcg gatagcgcca gtcttcacat
     8521 agacattcag ctggctgttg cctgcggtga tgtcataccc cagtatcatg ctggcacgcc
     8581 ccagcagcga ttcgtagtga ttcagatgta tattgaggcc attactcgcc ttcatagcca
     8641 tctcattctg gtggctgtat gtaagctggg tttgcggctc tatatagaac ccataaccag
     8701 taggggacag gttgaacctc tgcccggctt ccagggagat gctcattcca ttcgcagtgc
     8761 cgttggcgtt aacgccgttg ttctgactgt ccagtacgtg gaaactattt ttctggcgcg
     8821 atgcttttat aacgagatcg ctgtaaaaac cgttttgtgc catgtaactg gcgtacattc
     8881 ccatgtagtc tgaacgtgcg gtaccgtcgc ctccgctata gtccggcgat gcatgtgttg
     8941 agtcaatata cagaccgaca tacaacggca ttacatcaga gagacgttta tccccaccaa
     9001 actggatacc gctgtaaccc atgtcaaagc cgctcagttt gccactggca aaggagtcca
     9061 ggcttccccc ataactgcgc aaccagatat taccgtcttt actctgattt cgcaagtcac
     9121 ccatccgttg catcagcgta cggttttcaa catagttcaa taagtaaccg acattgagat
     9181 aattgccgcc agcatctgcg gtcgtggtgg gtttaggcgt gggagcgggt tcaggcgtag
     9241 gatcggggtt gactatggga ggctgagcgg gagctggtgt gggttcagga ttaggagtcg
     9301 gttccggaac tgtcccggaa gcgtaaagct cccagttagt gccattttta cgcacatcgt
     9361 acagatatcc ccccaactca acctgagaag acgcgctgaa cgaggccgcg ccgtcagtgg
     9421 tttttaccac tgtcagaact tcatttcccg ttgtggcctc gctgccctgg ttgcggatag
     9481 ccaatacgtg attaccagca ctactcccgc tgatattcaa taaatcccct ctattattaa
     9541 cgccattacc ctcgccaaca acatcagcac gcataataaa ggtactgtta ccgctcaggt
     9601 tctctacgtt taatgtggta aatgtgccgg cagttgaccc gtggctggca aaatcgacag
     9661 ttgaatggct cagcgccagc gtgtcgagat tagaattact tgttacgttc cagacgctgt
     9721 tattcattgc aacgtccagt ttcccgccat tgacattatc gctgagggag gaacctgtcc
     9781 aaaccgaccc agggtgcata tccagattga ttagcccacc tttggaaaga acgctaccgt
     9841 tgataagcat acgacccgag gcgttgatgc ggctggcggc ataaccatcg tcatgttgcg
     9901 ttgcgatggc catctggtcg ggtgtactca tatcaatgac cagatcgctc gtgaggtcga
     9961 tctggctgtt ggtcatggca taaatccctc tggctcctgc cgcgccggtg atagccaaac
    10021 tgtctccggt tattctaccg ccgctgagcg cccacaaacc cagcgccaga ctgccattac
    10081 gatcaaccgt aatatcggta ttttgcatgt tgataacagc cgttgccgtc tgggccgagg
    10141 caccataaga accgccggaa aagatgctgt ttcgttgcgc tgccgtgcca gaaaaattga
    10201 ttgtcgcgtc tgaactactg gtgacgaggc caccgccctg cgcggaagaa atatggctat
    10261 ctgcaccgat agtggttgta ccaccacgaa cttcgacgcc attggccgcg gctccagtta
    10321 catcaacagt gagtgcattc gcgctaacct ggccaaagct ccagaggccg tgagcattat
    10381 cgccattggt tttaatggta ctgtttgttc cgagatcgac aacagagttt ttctgtacgt
    10441 ttatccccat ggcgctgtag ccctgaacat cgattgtcag gtctgtcgcc gtaaaacgcg
    10501 cagcaccatt ggcgttattg ccgttgagac caccgatata aacacctgta cttccatcgg
    10561 tcttgatttt acttccgctt ccaagatcga cactggtgcc atagtcattg atggttaggc
    10621 ctataccgtt cgagttttca atggtgaatt gagtggctgt tagtgttgag ctatgcccaa
    10681 taatgatgcc gtcatcgtta ctcttaatgg tgctgcctgt gcctaagtca gcatgggtat
    10741 agtcaccaat taagttaatg ccgatggcag aggtttgacc aacaacatct actgtgagtc
    10801 ggttagctgt cagggagctg tttttgccct gaataatgat tccttttgca tatccagagg
    10861 cgtcgttgac attgacggtg acatcattac ccaggttgat attgccaggc gtgtttcctg
    10921 ctggggtcat tacaccatag aggtttcctg atggatcggc ggtatctcct gtaattctgt
    10981 caccatcatt aatagtaata ttggtgccat attgacttcc tgtatcagca gctatcagca
    11041 ggggggaaaa gataattgca ttcaaaccaa tagcgccaaa ttttatcagt tgaattgagg
    11101 gcgaacggat ttttaattca gcgagacgat cggctttggt attcccattg tgttgtttga
    11161 tgcccatacc actcctatat agtacccagg ctatgggtct atggattttg tcattttgtt
    11221 taagtcaatg aataatatcc tcttatcatc atgatatctg tatggtgcaa tcatgatgag
    11281 cttctctgag aataattctc tattaccggg caaaatcagg aacacaggaa ttaatatata
    11341 tttaaccccc cccttacaaa ggtgagttta tatcgagtta ttccctaagt tgtcgttact
    11401 tattccaatt cgttactatg aatatggcaa aaatattaca acaaacagcg gttaaaaaat
    11461 ttggtgaaga ttttttgtaa taatcctaat tagttatggg attttcgctt gatgtatctg
    11521 cataacaaaa acaatatgcg ccatttttgc attactaata agaaaaagca aaaaacatcg
    11581 atattcattt ttgttcaatg gattttctgg cctatcgtta ttattgtgct actggttatc
    11641 attttcatct tgccaggatc gcacgtaatc acgttattcg gggactttat ctgcgggata
    11701 ttacccttca ggtaagtcct tttctcgcca gcatattgga gcgagatcaa tattgatcag
    11761 ctgtgaagag ggctgatgtg tagtggaaca aaaactgacg ttatatcact gctaatgaga
    11821 tatcagtcat ctgaacactt atgtctttaa ttattctcgt ggttcactgt aggcaataag
    11881 cacaaaagtg taggatgtta caagaatgat taggactcgg tgaaatgaaa aatccacgca
    11941 attgcgtgga ttttatatac ttttgcgctc ttcatgagat ttagcgaaac ctcatgagac
    12001 aataaattaa ttagacgttg aaaaggaagt tcatcacatc gccatctttc acgatgtaat
    12061 ctttaccttc tgcacgcatt ttgcctgctt ctttcgcgcc ttgttcacct ttgtaagtga
    12121 tgaaatcttc aaacgagatg gtttgtgcac ggataaagcc tttttcaaaa tcagtatgga
    12181 ttttgcccgc tgcctgcggc gcggttgctc caaccggaat ggtccatgca cgcacttctt
    12241 tcaccccagc ggtgaagtaa gtttgcaggt tcagcagctt ataaccggca cggatcacac
    12301 ggttcaggcc cggttcttcc agcccaagct cctgcataaa ctcgtcacgt tcttcgtcgt
    12361 ccagttcggc aatgtctgct tcaacagcag cacaaaccgg aaccacaaca gaaccttctt
    12421 tcgccgcgat ttcacgcacc tggtcaagat atgggttgtt ttcaaaacca tcttcgttga
    12481 cgttggctat gtacattgtt ggtttcagcg tcaggaagct caggtaacga atagccgctt
    12541 tctcttcagc gcttaaatcc agcgcgcgca gcatacctgc gttttccagc tggggcaggc
    12601 atttttccag gaccgccagc tcagctttcg cgtctttatc gccacctttg gctttcttct
    12661 gtacgcgatg aatcgcacgt tcgcaggtgt cgaggtctgc cagcgccagt tcggtgttga
    12721 taacttcaat atcgtcagcc gggttaactt tgccggaaac gtgaatgatg ttgtcatttt
    12781 caaagcagcg aacaacgtga ccgatcgctt cggtttcacg gatgttggtc aggaactggt
    12841 tacccagacc ttcgcctttc gatgcgcctt ttaccagacc ggcgatatcg acaaattcca
    12901 tggtcgtggg aagcgtacgt tgcggtttta cgatttcagc cagttgatcc aggcgagggt
    12961 caggcattgg tacgacgcct gtgttcggct caatggtgca gaatggaaag ttggccgctt
    13021 caataccggc tttggtcagc gcgttgaaca gggtagattt cccgacgttg ggcaaaccga
    13081 cgataccgca tttgaatccc atgatttaaa tcaccttaat atcttaataa tcaacctgtt
    13141 attgctaaca gattgcagaa atggaaataa ctttgcctat tatacacggc actcggcaaa
    13201 aatgccgcag acaacgactt attgcgcttt aaaggcgtgc aatcggttcg ttgctttggt
    13261 caagccatct gtaaaccaca tttcagtaca acgcgccgct tcgtcgatgg cttcatcaat
    13321 taacttctgt tcactaacag gcggtttgcc taacacaaaa ccgacaactt tatttttatc
    13381 gcccggatga ccgattccga tgcgtaaacg gtgaaagtta gggttattac ccaatttact
    13441 gatgatgtct ttcagtccat tgtgaccacc atggccaccg cccaacttaa atttggcgac
    13501 gccaggaggc agatccagtt cgtcgtgggc caccagaatt tcgtccggat taatgcggaa
    13561 aaaactggcc atcgccgcaa cggctttgcc gctgagattc ataaatgtag tcgggactaa
    13621 caggcggaca tcttcgcttc caagggtgac tcgcgaagta taaccaaaga atttagcctc
    13681 ttcgcgcagc ggagcgcgca aacgctctgc cagtaagtca acgaaccagg caccagcatt
    13741 atgtcgcgtt gcggcgtatt cagcaccggg gttcgccagg ccgacaatca atttaatcgt
    13801 cacgtttttt tgtcctgagt gtgtacataa ctggcgcgta gtttactggt tgcggccccg
    13861 cttgacaaaa aactgcgtat caaatgcaga taacgtaata attgcctgag tggactatta
    13921 gaaagtcaag gtgttcaggc gtttatttgt aaagttttgt tgaaataagg gttgtaattg
    13981 tgattacgcc cgcacataac ccacagggtg ttgtctatac tttacacata aggaagaggg
    14041 gtattccctg ttacaaccca gaaagttccg gaggtgacat atgaaacgca aaaacgcttc
    14101 gatactcggt aacgtgctca tggggttggg tctggtggta atggtggtcg gcgtggggta
    14161 ttcaatcctc aaccagttac cacagtttaa tatgccccag tatttcgcac atggtgcagt
    14221 gctaagtatt ttcgtcggtg ccattctctg gctggcgggt gcccgtgttg gcgggcatga
    14281 acaggtgtgc gaccgttact ggtgggttcg tcactatgac aaacgttgcc gccgtagcga
    14341 taatcgccgt catagctaac aaataatgca gtttgctgac cagtcagttt gcgctgactg
    14401 gtcaatctcg tacttataaa tccgccatcg ccgcgcgacg attcgggaag aacgcgaggc
    14461 gtcccgggat cggttgaatg cctgcgcgag ccatagttcg cagtggctgg aattccacgt
    14521 tgcacacgcg cagttcacat ccttcgggca gacgcttcac aaaacgctgg aacgcatcaa
    14581 gaccaccagc atcaagtacc ggaacggcat cccacttcag aatcacaatc cgtttgcctt
    14641 caagacgtga ctccaggtcc gtgaataagc cttcagcagc agcaaaaaac agcgggccaa
    14701 taacgcgcag aaccaggaca tcgtctggaa catctacgac taccggtgcc aggcgagtca
    14761 tacgtgcgat acgacgcata aacagcagcg atgccagcac gatccccacg ctgatggcaa
    14821 taaccatatc aaacagcacg gtcagtgaca tacacagcag catgacgatg atgtcatctt
    14881 tcggcgcatg acgcagcaag tcgaccactt tgtgcgcttc actcatgttc cacgccacca
    14941 tcaacagcag ggctgccatg gcggaaagcg gcagccagga gagcagtggt gccagtacca
    15001 gcagggcgag aataaccaga atagagtgga tcaccgccga gataggggaa gttgccccgg
    15061 cacggacgtt agcggcagaa cgcgcgatgg cagcagtagc ggtaatacca ccaaagaatg
    15121 gagcgatgat attccccagc ccctgaccga ccagttcact attcgccttg tgcttagtcc
    15181 cggtcatacc atccagtacc acagcgcaga gcagagattc gatcgccccg agcattgcca
    15241 ttgagaatgc cgcaggcagc agggtgcgaa tagaatccca ggttagcgtg aattctgaat
    15301 taggcagatc ccacggcagc accagttgcg gcagcagttg cggaataccg ttaccctgag
    15361 aaccatcggc caggacgtag tggaattgcg aaccgatggt agcaacatgt ccgccgagca
    15421 ggttaacaat ccccatcacc gcgcaaccag ccagcaaggc cggaaggtga ccgggtaaac
    15481 gaatgcccag acgcggccag aaaaccagaa tgccaagcgt cacaatgcca atggcagcat
    15541 cacccacatt aatggtcggc agcgccataa ataatgcgcc gactttttgt agataatgtt
    15601 ccgggacatg ggccatttgc agaccgagaa aatctttaat ctgcatggta ccgatggtga
    15661 tcccgatacc cgaggtgaaa cctaaggtga cggaaaccgg aatatactca atcagacgac
    15721 caaagcgtgc cagccccatc agaatcaaaa agattcccga caacaaggtc gcaaccagca
    15781 gtcctgccag tccaaactgt tgcgaaacgg gatagagaat taccacaaat gccgcagtcg
    15841 gaccggaaac gctaaagcgt gacccacccg ttagagcaat gacaatcccc gcaacagctg
    15901 cggtatataa accgtactgg ggggccacac cactaccaat agccaacgcc atcgccagcg
    15961 ggatagcaat aatcccgacg gttatcccgg caatcaggtc acgggtaaac cgtgcggcag
    16021 tatatttttc tttccagcaa acgtcgatca gagcgcggaa aggcatcaca tgtgaggaaa
    16081 atattttgtt cacaataatg tttcatccgt gagcgcatca tctgtcaact aaatggcagg
    16141 tgaaggaggc ataggtcata caaatggata ttacagacaa aaaaacccgc cgcagcgggt
    16201 ctttgagccg ggttcgatta gtgttcgaac atggcagaga tcgattcttc gttgctgata
    16261 cgacgaatcg cttcggccag catacctgac agggtcagag tacgcacgtt cggcagtgat
    16321 ttgatttcat cgctcagcgg aatggtatcg cagacaacga cttcatcaat tacagagtta
    16381 cgcaggttgt tcgccgcgtt gccagagaag atcgggtgag tcgcgtacgc aaatacacgt
    16441 ttagcaccac gttctttcag cgcttcagca gctttacaca gcgtaccgcc agtgtcgatc
    16501 atatcatcga ccagtacgca gtcacgacct gcaacgtcac cgatgatatg catcacctgg
    16561 gaaacgttcg cacgcggacg acgtttgtcg atgattgcca tatcggtatc gttcagcagc
    16621 ttagcgatag cgcgggcacg cacaacgccg ccgatgtccg gagaaaccac aattgggtta
    16681 tccagattca gctgcagcat gtcttccagc aggatcgggc taccaaatac gttatcaacc
    16741 ggaacgtcga agaaaccctg aatctgttca gcgtgcagat ccactgtcag cacacggtca
    16801 acaccgacgc tggagaggaa gtctgcaacc actttcgcag tgattggtac acgagcggaa
    16861 cggacgcgac ggtcctggcg cgcatagcca aagtagggga taacagcggt gatacgacct
    16921 gcggaagcac gacgcagggc atcaaccata acgactaatt ccatcaggtt gtcgttagta
    16981 ggggcacaag tggactggat gatgaaaata tcaccaccgc gtacattttc gttaatttgt
    17041 acgctgactt cgccgtcgct aaagcgacct acagcggcgt cgccgagtga agtgtacagg
    17101 cggttggcaa tacgttgtgc tagtaccggg gtggcgttac cagcaaaaag cttcatatca
    17161 ggcacgagaa gaacctcagg catgcgtcca ttggtggaaa gaatctgccg aaaactgtgc
    17221 gggccaggca tgatcctttc caggcggtgt attaaagagc gcgatgcaac gtctggaaca
    17281 aggtgacgtt gtcaccgaaa ctcagcttgc ccggcttaaa gcatggctct gtgcaatggg
    17341 gaaagattaa cgcctttcgc cacaaagcca ttgagccatt ccggggcttg ctctagcacc
    17401 tggcgggctt cagactctgt atcaaattca gcaaagacac aggcccctgt cccagtcagg
    17461 cgtgacgggg cgtattctaa cagccaggaa agcaccgcat caacctcgcg aaaacgtttt
    17521 cttgcgataa cctcgcaatc attgctgaat tcacatttta gcaacgtttc tattgacctt
    17581 tttggcgtat tgcgcgggag ttcaggatct ttaaaaatca ccggagtcgg aatacttaca
    17641 ccagggtgcg ccaccagata ccacttctct ggcggatcca ccggcgttag tatttcacca
    17701 acgccttcgg caaacgcggc atgcccccga acaaagacag gaacatctgc gcccagcgtc
    17761 agccccattt ccgccagctc atccatgctt agcccgcatt gccagagatg atttaatgcc
    17821 accaggaccg tcgcggcatt ggatgaacca ccgccgagac cgccgcccat cggcaaacgc
    17881 ttgtcaatgc tgatattcgc accgcttccc gtcggaagac gcccgctgtc tgccgcagtt
    17941 ttcatcaaca accgcgctgc gcgaacgatc aggttatctt catgttccac gccttcaacg
    18001 ggcgttaaca gacgaatatc cccatcgtca cgaagctcaa tgctgatggt gtcgccgtaa
    18061 tcaagaaact gaaacagcgt ttgcagcgtg tggtaaccat ccgcacgctg accggtaatg
    18121 tataaaaaca gattaagttt tgccggagag ggccactgtg tccgcattat ttcactatcc
    18181 agttatccat ttttaacttg atgcgttgac caccgtcggt gagttccata ttggctggca
    18241 tcgcaggttg cgttttggtg tcataaccac cataaacaac cttccagttt ttgccattct
    18301 ggctgtaggt aatttcgctc aggcggtact ggtcgtccag tttgtagtcg gttgcatcac
    18361 ccggtaaacc taaaatccac tggcgcaagc tgttgagcgg aattggcatt ccggtcaatt
    18421 tgccaatcat ctcttcggcg tcatcggcgg tataacgctg acctttattg tcgactaact
    18481 gcacgttacc cggttgagca ttcagctcca gttccgtgct gcccaatggg ttagtgagca
    18541 gcagacggta gcgatcctgg ccggtttgct gccagaaaaa gcgggcgtac actttttgtt
    18601 ggtcagaaat ataagcgaac gcgccgcgag tctgatactg attaagattg cgcacgtctt
    18661 gctgatgctg acgccattgt ggcgaatccg ggcttttgcc aggacctttg ggcgtggtaa
    18721 cggaacaggc agtgagcaca agagcagcca gcggtagcag gcggataaga cgaaaatcgg
    18781 gcaggggcat agtgatgaca agtccttgag atacgttgca gttataaccc ttaatgctag
    18841 cgttaccgtc cgctatcgtc tatgttcaag ttgtcttaat tgccagaatc taacggcttt
    18901 cggcaattac tccaaaaggg ggcgctctct tttattgatc ttacgcatcc tgtatgatgc
    18961 aagcagacta accctatcaa cgttggtatt atttcccgca gacatgaccc ttttagcact
    19021 cggtatcaac cataaaacgg cacctgtatc gctgcgagaa cgtgtatcgt tttcgccgga
    19081 taagctcgat caggcgcttg acagcctgct tgcgcagccg atggtgcagg gcggcgtggt
    19141 gctgtcgacg tgcaaccgca cggaacttta tcttagcgtt gaagagcagg ataacctgca
    19201 agaggcgtta atccgctggc tttgcgatta tcacaatctt aatgaagaag atctgcgtaa
    19261 aagcctctac tggcatcagg ataacgacgc ggttagccat ttaatgcgtg ttgccagcgg
    19321 cctggattca ctggttcttg gggagccgca gatcctcggt caggttaaaa aagcgtttgt
    19381 cgattcgcaa aaaggtcata tgaaggccag cgaactggaa cgcatgttcc agaaatcttt
    19441 ctctgtagcg aaacgcgttc gcactgaaac agatatcggt gccagtgctg tgtctgtcgc
    19501 ttttgcggct tgtacgctgg cgcggcagat ctttgaatcg ctctctacgg tcacagtgtt
    19561 gctggtaggc gcgggcgaaa ccatcgagct ggtagcgcgt catctgcgcg aacataaagt
    19621 acagaagatg attatcgcca accgcactcg cgaacgtgcc caaattctgg cagatgaagt
    19681 tggcgcggaa gtgattgccc tgagtgagat cgacgaacgt ctgcgcgaag ccgatatcat
    19741 catcagttcc accgccagcc cgttaccgat tatcgggaaa ggcatggtgg agcgcgcatt
    19801 aaaaagtcgt cgtaaccaac caatgctgtt ggtggatatt gccgttccgc gcgatgttga
    19861 gccggaagtt ggcaaactgg cgaatgctta tctttatagt gtggacgatc tgcaaagcat
    19921 catttcgcac aacctggcgc agcgtaaagc cgcagcggtt gaggcggaaa ctattgtcgc
    19981 tcaggaaacc agcgaattta tggcgtggct gcgagcacaa agcgccagcg aaaccattcg
    20041 cgagtatcgc agccaggcag agcacgttcg cgatgagtta accgccaaag cgttagcggc
    20101 ccttgagcag ggcggcgacg cgcaagccat tatgcaggat ctggcatgga aactgactaa
    20161 ccgcttgatc catgcgccaa cgaaatcact tcaacaggcc gcccgtgacg gggataacga
    20221 acgcctgaat attctgcgcg acagcctcgg gctggagtag cagtacatca ttttcttttt
    20281 ttacagggtg catttacgcc tatgaagcct tctatcgttg ccaaactgga agccctgcat
    20341 gaacgccatg aagaagttca ggcgttgctg ggtgacgcgc aaactatcgc cgaccaggaa
    20401 cgttttcgcg cattatcacg cgaatatgcg cagttaagtg atgtttcgcg ctgttttacc
    20461 gactggcaac aggttcagga agatatcgaa accgcacaga tgatgctcga cgatcctgaa
    20521 atgcgtgaga tggcgcagga tgaactgcgc gaagctaaag aaaaaagcga gcaactggaa
    20581 cagcaattac aggttctatt actaccaaaa gatcctgatg acgaacgtaa cgccttcctc
    20641 gaagtccgtg ccggaaccgg cggcgacgaa gcagcgctgt ttgcaggcga tctgttccgt
    20701 atgtacagcc gttacgccga agcccgccgc tggcgggtag aaatcatgag cgccagcgag
    20761 ggtgaacatg gtggttataa agagatcatc gccaaaatta gcggtgatgg tgtgtatggt
    20821 cgtctgaaat ttgaatccgg cggtcatcgc gtgcaacgtg ttcctgctac ggaatcgcag
    20881 ggtcgtattc atacttctgc ttgtaccgtt gcggtaatgc cagaactgcc tgacgcagaa
    20941 ctgccggaca tcaacccagc agatttacgc attgatactt tccgctcgtc aggggcgggt
    21001 ggtcagcacg ttaacaccac cgattcggca attcgtatta ctcacttgcc gaccgggatt
    21061 gttgttgaat gtcaggacga acgttcacaa cataaaaaca aagcgaaggc gctttcagtg
    21121 ctcggtgctc gcatccacgc tgctgaaatg gcaaaacgcc aacaggccga agcgtctacc
    21181 cgtcgtaacc tgctggggag tggcgatcgc agcgaccgta accgtactta caatttcccg
    21241 caggggcgcg ttaccgatca ccgcatcaac ctgacgctct accgcctgga tgaagtgatg
    21301 gaaggtaagc tggatatgct gattgaaccg attatccagg aacatcaggc cgaccaactg
    21361 gcggcgttgt ccgagcagga ataatggaat atcaacactg gttacgtgaa gcaataagcc
    21421 aacttcaggc gagcgaaagc ccgcggcgtg atgctgaaat cctgctggag catgttaccg
    21481 gcagagggcg tacttttatt ctcgcctttg gtgaaacgca gctgactgac gaacaatgtc
    21541 agcaacttga tgcgctactg acacgtcgtc gcgatggtga acccattgct catttaaccg
    21601 gggtgcgaga attctggtcg ttgccgttat ttgtttcgcc agcgacctta attccgcgcc
    21661 cggatacgga gtgtctggtg gagcaggcac tggcgcggtt gcctgaacaa ccttgccgta
    21721 ttctcgatct cgggacgggt accggggcga ttgcgcttgc gctggctagc gagcgcccgg
    21781 actgcgaaat tatcgctgta gatcgtatgc ctgatgctgt ctccctggca caacgtaatg
    21841 cccagcatct ggcgatcaaa aatatccaca ttctgcaaag cgactggttt agcgcgctag
    21901 ccgggcagca gtttgcgatg attgtcagca atccgccgta tattgacgag caggacccac
    21961 atcttcaaca aggcgatgtc cgctttgagc cgctcactgc gctggttgcg gcagacagtg
    22021 gaatggcaga catcgtgcat atcatcgaac agtcgcgtaa cgcgctggta tccggcggct
    22081 ttctgcttct ggaacatggc tggcagcagg gcgaagcggt gcgacaagca tttatcctcg
    22141 cggggtatca tgacgtcgaa acctgccgtg actatggtga taacgagcgc gtaacgctcg
    22201 gccgctatta tcaatgacaa gtttttctac actgcttagt gttcatctta ttagtatcgc
    22261 gctttctgtt gggctattaa ccttacgttt ctggctacgt tatcagaagc atccacaggc
    22321 atttgctcgc tggacgcgca ttgtgccgcc ggttgtcgat acggtgttat tgttaagcgg
    22381 cattgcgttg atggctaaag cgcacatcct gccattttcc gggcaggcac agtggctgac
    22441 tgaaaagctg tttggagtta tcatttatat cgttttgggt tttattgcac tcgattatcg
    22501 tcgtatgcac agtcagcagg cgcgcattat tgccttcccg ctggcgttgg tggtgctgta
    22561 catcatcatt aaactcgcca ccacaaaagt accgttactg gggtaagtca tgagatcgtt
    22621 agctgatttc gaatttaata aagcgccatt gtgcgaaggc atgatcctgg cttgcgaagc
    22681 aatccgccgc gattttccct cgcaagatgt ttacgacgaa ctggagcgtc tcgttagtct
    22741 ggcgaaggaa gaaatcagcc agcttctgcc tttagaagag cagctggaaa aactgatcgc
    22801 gctgttttac ggcgactggg gatttaaagc ctcacgcggc gtttatcgtc tttccgatgc
    22861 attatggctg gaccaggtgt taaagaatcg acagggcagt gcggtatcat taggtgcggt
    22921 tttattatgg gtcgcgaatc gtctcgattt gccgctgctg ccggtgattt tccctacgca
    22981 gctgatattg cgcattgaat gtccggatgg cgaaatttgg ctgattaatc cttttaacgg
    23041 tgaatcgtta agcgaacata tgctggacgg atggttaaag ggaaatatca gcccgtcggc
    23101 ggaactgttt tatgaagacc ttgatgaagc tgataacatt gaggtaatcc gcaaattgct
    23161 ggatacactc aaagcctcgt tgatggaaga aaatcagatg gagctggcgt tacgcaccag
    23221 cgaagcttta ttacaattca accctgaaga tccctatgaa attcgcgatc gtgggttgat
    23281 ttatgcgcaa ctggattgcg aacacgttgc gttgaacgat ttaagttatt tcgttgaacg
    23341 gtgtccggaa gacccgatca gcgaaatgat ccgtgcgcaa ataaataaca tcgcgcataa
    23401 acatattgtg ctgcattaat taatcgacat tttactcaag attaaggcga tcctatgaaa
    23461 caaaaagtgg ttagcattgg cgacatcaac gtagcaaatg acctgccgtt cgtactgttt
    23521 ggcggtatga acgtgctgga atctcgcgat ctggcgatgc gcatttgcga gcactacgta
    23581 actgtgaccc agaaactggg tatcccttac gtgttcaaag cctcttttga caaagccaac
    23641 cgctcctcca tccactctta tcgtggaccg ggcctggaag aagggatgaa aatcttccag
    23701 gagttgaagc agacttttgg cgtgaaaatt atcaccgacg ttcacgaacc aagtcaggca
    23761 cagcccgttg ctgatgtcgt ggatgtgatt cagttgccgg cgtttcttgc tcgccagact
    23821 gacctggttg aagccatggc gaaaaccggt gcggtaatta acgtcaagaa accacagttt
    23881 gtcagcccgg gacagatggg taatatcgtt gataaattca aagaaggcgg caacgaaaaa
    23941 gtgattcttt gcgatcgtgg tgctaacttc ggctatgaca acctggttgt cgatatgctg
    24001 ggcttcagca ttatgaagaa agtgtctggt aactcgccgg tgattttcga cgtgacccac
    24061 gcactgcaat gccgcgatcc gtttggcgca gcttccggtg gtcgtcgtgc tcaggtggct
    24121 gagctggcac gagccggtat ggcggtaggt ctggcggggc tgtttattga agcgcatccg
    24181 gatccggaac atgcgaaatg tgatggtcca tccgcgctgc cgctggctaa actggaaccg
    24241 ttcctcaagc agatgaaagc gattgatgat ctggtgaaag gtttcgaaga actggatacc
    24301 agcaagtaat cttttttgct tgaaaaataa agtcttagcg ttctgcgtta agactttttt
    24361 catgggtgcc agatacaaaa aaggccgcag gctgttaccc ctgcggccgg tttcgggcgc
    24421 atattgccat cacggcagcc tgacgcccgt tttcacctta cttccggtta cgccaccagc
    24481 tgacaatcgc tgcggcaata atccccgcca ggatcggtgc tgccaggtcg tgccagaaag
    24541 tcatggcaaa ctgcgcgagc gtcatatagc cgccttgttg taatgacaac attttgcggc
    24601 tattcttgaa gtgtctggtt tcaagattag cccccgtttt gttgtcaggt tttacctctc
    24661 aacgtgcggg ggttttctct ttccagcaac caatgccacc agggataaag cccccgcaac
    24721 attgcgcctc accggataat tccggcttgg tgtggatact acgtctcaat tcatcttcac
    24781 ttcatccctg aaatgtttgc aataaagagt acattccggc ttttcaacag ctgttgcagt
    24841 ggtttcgtgc aggttgtggt acaggctcgc aattctgatt aacgagctgt ccaggtagta
    24901 tgaagaaata caataagcag gaatttatct tatctctccc gatttccgtc atcggaggag
    24961 atataactgt caggcaaata tcgtcatcaa ataggcggca aacagtgcca gatgcgctgc
    25021 gccattgagc acgttagtac gtccggtgga gaaggagata tggcacagca ctaaagaggc
    25081 caccatcacc accatttctg gcgcaccaag tgcaaactgc aattcgttac ccgtcataaa
    25141 ggcaattagc gtgacgacag gtacggtaag cgaaattgtt gctaacactg aaccaaagaa
    25201 cagattcatc gcgcgctgaa cctggttgtt caacactgct tttaacgcac ctaaaccttc
    25261 cggcgacaga atcaacagtg ccaccaggaa gccagtaaag gcaacagggg cattcatgct
    25321 gtcgagcaat gtctccagcg agctggcgtt cattttggtg actgcaataa cggcaatcag
    25381 atggataatc aaccagatag catgccacag gctgctatgg gcagacgttt taccgtggtg
    25441 cgggtcgtcg tcatcactgt catcttcgtg ctcgtagaca aacaaacttt gatgcgtttt
    25501 ggcctggatc agcaaaaata cgccatacat tgccgcagaa attaatgcta ccagtaacgc
    25561 ctgaccggtt gaaaaattcg ccgcaggcag agccattgga aataccagta cgattatcgc
    25621 cagggggaac agcgcaatta aatactgctt gataccaaac agattcatat attgggtggc
    25681 aaacttacga ccgcccaaca ataatgaaaa gccaaccagc ccaccggtaa caatcataat
    25741 gattgaatag agcgtatcac gcattagcgt tggcgcggcg tcgccggttg ccattaaagc
    25801 tgaaatcaaa ctgacttcaa gaataaccac tgaaaggcta agaataagcg aaccgtaagg
    25861 ttctcccagg cgatgggcta atacgtccgc atgacggaca acactaaagg cgctacttaa
    25921 aataccaata agcgcaagaa gattgatggc aatgaccact ggtagtgtct ggctacttcc
    25981 ccacaggaac agcactacca gcaccagaac cgggaaaata agcgaagtct ccttgtggcg
    26041 ggtttttacc gcctcttgag catttgacat tatggttatc cctttgcaga tgaatttatc
    26101 gaaaatgtaa aaaataggta g
//
LOCUS       DAZOYF010000044        25659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZOYF010000044 DAZOYF010000000
VERSION     DAZOYF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.044"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHUOF_001722"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHUOF_001722"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR0610936.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="ACHUOF_001723"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="ACHUOF_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR0610937.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="ACHUOF_001724"
     CDS             2630..3391
                     /locus_tag="ACHUOF_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR0610938.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="ACHUOF_001725"
     CDS             complement(3442..4326)
                     /locus_tag="ACHUOF_001725"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR0610939.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHUOF_001726"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHUOF_001726"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR0610940.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="ACHUOF_001727"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="ACHUOF_001727"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR0610941.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="ACHUOF_001728"
     CDS             6202..6474
                     /locus_tag="ACHUOF_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR0610942.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="ACHUOF_001729"
     CDS             6844..7920
                     /locus_tag="ACHUOF_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR0610943.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="ACHUOF_001730"
     CDS             7947..9326
                     /locus_tag="ACHUOF_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0610944.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="ACHUOF_001731"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="ACHUOF_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="ACHUOF_001732"
     CDS             10409..11245
                     /locus_tag="ACHUOF_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFR0610945.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="ACHUOF_001733"
     CDS             11250..12197
                     /locus_tag="ACHUOF_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR0610946.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHUOF_001734"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHUOF_001734"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFR0610947.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="ACHUOF_001735"
     CDS             13324..14082
                     /locus_tag="ACHUOF_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0610948.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="ACHUOF_001736"
     CDS             14200..15558
                     /locus_tag="ACHUOF_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0610949.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACHUOF_001737"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACHUOF_001737"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR0610950.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACHUOF_001738"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACHUOF_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR0610951.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACHUOF_001739"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACHUOF_001739"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR0610952.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="ACHUOF_001740"
     CDS             19503..20054
                     /locus_tag="ACHUOF_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR0610953.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="ACHUOF_001741"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="ACHUOF_001741"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR0610954.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="ACHUOF_001742"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="ACHUOF_001742"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR0610955.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="ACHUOF_001743"
     CDS             23307..24347
                     /locus_tag="ACHUOF_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0610956.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACHUOF_001744"
     CDS             complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACHUOF_001744"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR0610957.1"
                     /translation="MERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
BASE COUNT         6517 a         5971 c         6624 g         6547 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 taatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatatgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagta
//
LOCUS       DAZOYF010000045        25592 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZOYF010000045 DAZOYF010000000
VERSION     DAZOYF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.045"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="ACHUOF_001745"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="ACHUOF_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHUOF_001746"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHUOF_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR0610958.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFVSGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHUOF_001747"
     CDS             complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHUOF_001747"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR0610959.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHUOF_001748"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHUOF_001748"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR0610960.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="ACHUOF_001749"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="ACHUOF_001749"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR0610961.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="ACHUOF_001750"
     tRNA            7783..7858
                     /locus_tag="ACHUOF_001750"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
     gene            8016..8091
                     /locus_tag="ACHUOF_001751"
     tRNA            8016..8091
                     /locus_tag="ACHUOF_001751"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8049..8051,aa:Gly,seq:gcc)
     gene            8127..8202
                     /locus_tag="ACHUOF_001752"
     tRNA            8127..8202
                     /locus_tag="ACHUOF_001752"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8160..8162,aa:Gly,seq:gcc)
     gene            8436..8489
                     /locus_tag="ACHUOF_001753"
     CDS             8436..8489
                     /locus_tag="ACHUOF_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610962.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(8472..9611)
                     /gene="queG"
                     /locus_tag="ACHUOF_001754"
     CDS             complement(8472..9611)
                     /gene="queG"
                     /locus_tag="ACHUOF_001754"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR0610963.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            9610..11157
                     /gene="nnr"
                     /locus_tag="ACHUOF_001755"
     CDS             9610..11157
                     /gene="nnr"
                     /locus_tag="ACHUOF_001755"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR0610964.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            11129..11590
                     /gene="tsaE"
                     /locus_tag="ACHUOF_001756"
     CDS             11129..11590
                     /gene="tsaE"
                     /locus_tag="ACHUOF_001756"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR0610965.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            11609..12946
                     /gene="amiB"
                     /locus_tag="ACHUOF_001757"
     CDS             11609..12946
                     /gene="amiB"
                     /locus_tag="ACHUOF_001757"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR0610966.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            12956..14803
                     /gene="mutL"
                     /locus_tag="ACHUOF_001758"
     CDS             12956..14803
                     /gene="mutL"
                     /locus_tag="ACHUOF_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR0610967.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            14796..15746
                     /gene="miaA"
                     /locus_tag="ACHUOF_001759"
     CDS             14796..15746
                     /gene="miaA"
                     /locus_tag="ACHUOF_001759"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR0610968.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            15832..16140
                     /gene="hfq"
                     /locus_tag="ACHUOF_001760"
     CDS             15832..16140
                     /gene="hfq"
                     /locus_tag="ACHUOF_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR0610969.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            16216..17496
                     /gene="hflX"
                     /locus_tag="ACHUOF_001761"
     CDS             16216..17496
                     /gene="hflX"
                     /locus_tag="ACHUOF_001761"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR0610970.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            17582..18841
                     /gene="hflK"
                     /locus_tag="ACHUOF_001762"
     CDS             17582..18841
                     /gene="hflK"
                     /locus_tag="ACHUOF_001762"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR0610971.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            18844..19848
                     /gene="hflC"
                     /locus_tag="ACHUOF_001763"
     CDS             18844..19848
                     /gene="hflC"
                     /locus_tag="ACHUOF_001763"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR0610972.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            19930..20127
                     /locus_tag="ACHUOF_001764"
     CDS             19930..20127
                     /locus_tag="ACHUOF_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR0610973.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            20231..21529
                     /gene="purA"
                     /locus_tag="ACHUOF_001765"
     CDS             20231..21529
                     /gene="purA"
                     /locus_tag="ACHUOF_001765"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR0610974.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            21734..22159
                     /gene="nsrR"
                     /locus_tag="ACHUOF_001766"
     CDS             21734..22159
                     /gene="nsrR"
                     /locus_tag="ACHUOF_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR0610975.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            22198..24639
                     /gene="rnr"
                     /locus_tag="ACHUOF_001767"
     CDS             22198..24639
                     /gene="rnr"
                     /locus_tag="ACHUOF_001767"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR0610976.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            24819..25550
                     /gene="rlmB"
                     /locus_tag="ACHUOF_001768"
     CDS             24819..25550
                     /gene="rlmB"
                     /locus_tag="ACHUOF_001768"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR0610977.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT         5992 a         6486 c         7185 g         5929 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagaca
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttgaaaag tgctgcaaag cacagaccac ccaatcgcag tatttcgaat tgcgacaagg
     7921 cggcaactga acgaatcctt gggaacttac aaaagtatgt gactaaggtg agtgaaggca
     7981 gccaaggcag tagcggttcg aaagacgaag attaagcggg aatagctcag ttggtagagc
     8041 acgaccttgc caaggtcggg gtcgcgagtt cgagtctcgt ttcccgctcc aaaatttaaa
     8101 agtgctgtaa ggcatagacc acccaagcgg gaatagctca gttggtagag cacgaccttg
     8161 ccaaggtcgg ggtcgcgagt tcgagtctcg tttcccgctc caaattcttc tctcaataaa
     8221 atatctacag cgacgcgatg cgttattgct ggtttttgtt gtctctgaca aactcttgta
     8281 aacagagtta tccacagcct caggctgtaa tcttaatttc aaagaaactt cgcacggtga
     8341 atggtatttt tttaacctat tgataaataa gttaaaaatc aagattccgt tttgtcgagt
     8401 cgatcacttg acgattttat tcgtcttgaa ttgcaatgcg tttttatttt tattcacaag
     8461 ctgtggatga atcaggcgtc acgcggtaac cctttttcaa tcacccgaac cagacgctgt
     8521 tttttcggca attgcacttc gactatgcac gcatttcgtc tctctatttg ctgcgcaatc
     8581 gcccacgcta tgtgctcatc gagaagtggg tgctcacctt tacgactttc cagcgctgtc
     8641 aaaatcgttt catcccaagg ggcattgcct aatgccacgg cgatattacg cagccagcgc
     8701 aggtgaccta tacggcgaat cgccgatcct tccgtgactt ttaaaaactt ctcttcgctc
     8761 caggcaaata actcaatgag ttccggtgcg tgtagcggct tacgcgggct gaaatcgtct
     8821 tctgtagtga gttgcgaata gcgattccac gggcagataa gctggcagtc atcgcaaccg
     8881 taaatacggt ttcccattaa cggtcgcaac tcttccggga gcgccccttc aagttcgatg
     8941 gtgagataag agatacagcg gcgagcatcg acggtatatg gctcgacgat ggcaccggtc
     9001 gggcaaatcg tcatacaggc cacgcatttg ccgcatcctt cctcgactgg ttgatccacg
     9061 ggcagcggaa tatcgaccag caattcgcct aaaaagaaga acgaaccggc ctcgcgattg
     9121 aggataagtg agtgcttacc tgtccagccg agcccagctt tttcagctaa cgggcgctcg
     9181 agaataggcg cagaatcgac aaacggtcta aaattcagcg aaacacaatg ttgctgaatc
     9241 atctcgccca gctttttgag tcggttgcgc agaagtttgt gatagtcacg gcccagcgca
     9301 taacggctaa catagccgag tttggggttt ttcagcgtgc tggcaaatgc ggcgttagca
     9361 ggaaggtaat tcatccgcac gctgattaca cgcagcgtac cgggtaataa ctcatggggg
     9421 cgagcgcgca gcataccgtg acgtgccatc cagtccattt cgccgtggta ttgtttgtcc
     9481 agccatgctt gcagtttggg ctcggactcg ctgagatcgg tatcggtaat acctacctgc
     9541 tgaaagccca gttccagccc ccactgttta attttttgcg ctaactgatt gagatcgagg
     9601 ggctctgaca tgacggacca tacaatgaag aaaaaccccg taagtatacc acacaccgtc
     9661 tggtacgccg acgatatccg ccgcggagaa cgcgaggcgg cagatgtgct ggggctcaca
     9721 ctctatgagc tgatgcttcg cgctggcgag gccgcattcc aggtgtgtcg ttcggcgtat
     9781 cctgacgccc gccactggct ggtgctgtgc ggtcatggta ataacggcgg cgatggctac
     9841 gtggtcgcgc gactggccaa agcggtcggc attgaggtca cgttgttggc ccaggagagc
     9901 gacaaaccgt tgccggaaga ggccgcgctg gcacgcgaag catggttaaa cgcgggtggc
     9961 gagatccatg cttcgaatat tgtctggccc gaatcggtag atctgattgt tgatgcgctg
    10021 ctcggtaccg gcttgcagca agcgccccgc gaatccatta gccagttaat cgaccacgct
    10081 aattcccatc ctgcgccgat tgtggcggtt gatatccctt ccggcctgct ggctgaaacc
    10141 ggcgctacgc caggcgcagt gatcaacgcc gatcacacca tcacttttat tgcgctgaaa
    10201 ctaggcttgc tcactggaaa agcgcgggat gttacaggac aactgcattt tgactcactg
    10261 gggctggata gctggctggc aggtcaggag acgaaaattc agcggttttc ggcagaacaa
    10321 ctttctcact ggctgaaacc gcgtcgcccg acttcgcata aaggcgatca cgggcggctg
    10381 gtgattatcg gtggcgatca cggcacggcg ggggctattc gtatgacggg ggaagcggcg
    10441 ctacgtgctg gtgctggttt agtccgagta ctgacccgca gtgagaacat tgcgccgctg
    10501 ctgactgcac gaccagaatt gatggtgcat gaactgacta tggactctct taccgaaagc
    10561 ctggaatggg ccgatgtggt ggtgattggt cccggtctgg gccagcaaga gtgggggaaa
    10621 aaagccctgc aaaaagttga gaattttcgc aaaccgatgt tgtgggatgc cgatgcattg
    10681 aacctgctgg caatcaatcc cgataagcgt cacaatcgcg tgatcacgcc gcatcctagc
    10741 gaggccgcac ggttgttagg ctgttccgtc gctgaaattg aaagtgaccg cttacattgc
    10801 gccaaacgtc tggtacaacg ttatggcggc gtagcggtgc tgaaaggtgc cggaaccgtg
    10861 gtcgccgccc atcctgacgc tttaggcatt attgatgccg gaaatgcagg catggcgagc
    10921 ggcggcatgg gcgatgtgct ctctggtatt attggcgcat tgcttgggca aaaactgtcg
    10981 ccgtatgatg cagcctgtgc aggctgtgtc gcgcacggtg cggcagctga cgtactggcg
    11041 gcgcgttttg gaacgcgcgg gatgctggca accgatctct tttccacgct acagcgtatt
    11101 gttaacccgg aagtgactga taaaaaccat gatgaatcga gtaattccgc tccctgatga
    11161 gcaggcaaca ttagacctgg gcgagcgggt agcgaaagcc tgcgatggcg caaccgtaat
    11221 ctatctgtat ggcgatttag gcgcaggtaa aaccaccttt agccggggct ttttacaggc
    11281 tctgggtcat cagggtaatg tcaaaagccc cacttatacg ctggtcgaac cctatacgct
    11341 cgacaactta atggtctatc actttgattt gtaccgcctt gccgatcccg aggagctgga
    11401 gtttatgggg atccgcgatt attttgccaa cgatgccatc tgcctggtgg agtggccaca
    11461 acaaggtaca ggtgttcttc ctgacccgga tgtcgaaata cacattgatt atcaggcaca
    11521 aggccgtgag gcgcgcgtga gtgcggtttc ctctgcgggt gaattgttgc tggcgcgttt
    11581 agccggttaa cctttgaaag gtggcgggat gatgtatcgc atcagaaatt ggttggtagc
    11641 gacgctgctg ctgctgtgca cgccggtggg tgccgcgacg ctctctgata ttcaggtttc
    11701 taacggtaat caacaggcgc ggataacgtt gagttttatt ggcgatcctg attatgcgtt
    11761 tagccatcaa agcaaacgca ccgtggcgct cgatatcaaa caaacgggca tgattcaggg
    11821 actgccgttg ttgttcagcg gcaataatct ggtgaaggcg attcgctctg gaacgcctaa
    11881 agatgcacaa acgctacggc tggtggtcga tcttaccgaa aacggtaaaa ccgaagcggt
    11941 gaagcggcag aatggcagca attacactgt cgtctttacg attaacgccg atgtgccgcc
    12001 accgcctcct ccgccgcccg tggttgcgaa acgcgttgaa acgcctgcgg ttgtcgcacc
    12061 gcgcgtcagc gaaccggcgc gcaatccgtt taaaacggaa agtaaccgca ctacgggtgt
    12121 tatcagcagt aatacggtaa cgcgtccggc agcgcgcgcg acggctaaca ctggcgataa
    12181 aattatcatc gctattgatg ccggacacgg cggtcaggac cctggcgcta tcggccccgg
    12241 tggtacgcgg gagaaaaatg tcaccatcgc catcgcgcgt aaattgcgta ctttgctcaa
    12301 tgacgatccg atgtttaaag gcgttttaac ccgtgacggg gattacttta tctcggtgat
    12361 ggggcgcagt gatgtggcac ataagcaaaa cgccaatttc ctcgtgtcga ttcacgctga
    12421 tgccgcaccg aaccgtagtg cgactggcgc ttccgtatgg gtgctctcta accgtcgcgc
    12481 caacagtgaa atggccagct ggctggagca gcacgagaaa ctgtcggagc tgctgggtgg
    12541 ggcgggtgat gtgctggcga acagtcagtc tgacccctat ttaagccagg cggtgctgga
    12601 tttacagttc ggtcattccc agcgggtagg gtatgatgta gcgaccagta tgatcagtca
    12661 gttgcaacgc attggcgaaa tacataaacg tcgaccagaa cacgccagcc ttggcgttct
    12721 gcgctcgccg gatatcccat cagtactggt cgaaaccggt tttatcagca acaacagcga
    12781 agaacgtttg ctggcgagcg acgattacca acaacagctg gcagaagcca tttacaaagg
    12841 cctgcgcaat tacttccttg cgcatccgat gcaatctgcg ccgcagggtg caacggcaca
    12901 aactgccagt acggtgacga cgccagatcg cacgctgcca aactaaggac gattgatgcc
    12961 aattcaggtc ttaccgccac aactggcgaa ccagattgcc gcaggtgagg tggtcgagcg
    13021 acctgcgtcg gtagtcaaag aactagtgga aaacagcctc gatgcaggtg cgacgcgtat
    13081 cgatattgat atcgaacgcg gtggggcgaa acttatccgc attcgtgata acggctgcgg
    13141 tatcaaaaaa gatgagctgg cgctggcgct ggctcgtcat gccaccagta aaatcgcctc
    13201 tctggacgat ctcgaagcca ttatcagcct gggctttcgc ggtgaggcgc tggcgagtat
    13261 cagttcggtt tcccgcctga cgctcacttc acgcaccgca gaacagcagg aagcctggca
    13321 ggcctatgcc gaaggacgcg acatggacgt gacggttaaa ccggcagcgc atccggtggg
    13381 aacgacgctg gaggtgctgg atctgttcta caacaccccg gcgcggcgca aattcctgcg
    13441 caccgagaaa accgaattta gccatattga tgaaatcatc cgccgcattg cgctggcgcg
    13501 tttcgacgtc acgatcaacc tgtcgcataa cggtaaaatt gtgcgtcagt accgcgcagt
    13561 gccggaaggc gggcaaaaag aacggcgctt aggcgcgatt tgcggcaccg cttttctcga
    13621 acaagcgctg gcgattgaat ggcaacacgg cgatctcacc ctgcgcggct gggtggccga
    13681 tccaaatcac actacgcccg cactggcgga aattcagtat tgctacgtga atggtcgcat
    13741 gatgcgcgat cgcctgatca atcatgcgat ccgccaggcc tgcgaagaca aactgggggc
    13801 cgatcagcaa ccggcatttg tgttgtatct ggagatcgac ccgcatcagg tggacgtcaa
    13861 cgtacacccc gccaaacacg aagtgcgttt ccatcagtcg cgtctggtgc atgattttat
    13921 ctatcagggc gtgctgagcg tgctacaaca gcaactggaa acgccgctac cgctggacga
    13981 tgaaccacaa cccgcaccgc gtgccatccc ggaaaaccgc gtggcggcgg ggcgcaatca
    14041 ctttgcagaa ccggcagctc gtgagccggt agctccgcgc tactctcctg cgcccgcctc
    14101 aggcagtcgt ccggctgccc cctggccgaa tgcgcagcca ggctaccaga aacagcaagg
    14161 tgaagtgtat cgccagcttt tgcaaacgcc cgcgccgatg caaaaaccaa aagcgccgga
    14221 accgcaggaa cctgcacttg cggcgaacag tcagagtttt ggtcgggtac tgactatcgt
    14281 ccattccgac tgtgcgttgc tggagcgcga tggcaacatt tcacttttat ccttgccagt
    14341 ggcagaacgt tggctgcgtc aggcacaatt gacgccgggt gaagcgcccg tttgcgccca
    14401 gccgctgctg attccgttgc ggctaaaagt ttctggcgaa gaaaaatcgg cattagaaaa
    14461 agcgcagtct gccctagcgg aattgggtat tgatttccag tcagatgcac agcatgtgac
    14521 catcagggcc gtgcctttac ccttacgcca acaaaattta caaatcttga ttcctgaact
    14581 gataggctac ctggcgaagc agtccgtatt cgaacctggc aatattgcgc agtggattgc
    14641 acgaaatctg atgagcgaac atgcgcagtg gtcaatggca caggccataa ccctgctggc
    14701 ggacgtggaa cggttatgtc cgcaacttgt gaaaacgccg ccgggtggtc tgttacaatc
    14761 tgttgattta catccggcga taaaagccct gaaagatgag tgatatcagt aaggcgagcc
    14821 tgcctaaggc gatttttttg atggggccga cggcctccgg taaaacggcg ttagccattg
    14881 agctgcgtaa aattttacca gtagagttga taagcgttga ttctgtcctt atttacaaag
    14941 ggatggatat cgggacggcg aagccgaacg ctgaagagtt actcgccgcg ccgcaccgat
    15001 tgctggatat tcgcgatccg tcgcaggctt actcggctgc tgattttcgc cgcgatgcgc
    15061 tggcggaaat ggccgatatc accgcggcgg ggcggatccc actgttagtg ggcggtacga
    15121 tgttgtattt caaggcattg ctggaagggt tgtcgccgct accgtcggca gacccggaag
    15181 tgcgggccag aattgagcaa caggcggcag agcaaggttg ggagtcattg catcgtcaac
    15241 ttcaggaggt agatccggtt gcggcagcaa ggattcatcc aaatgatcca caaaggcttt
    15301 cccgggcact ggaagttttt ttcatttcgg gtaaaacttt aacggaactg acgcaaacgt
    15361 caggagacgc tctaccgtat caggtgcatc agttcgccat cgccccggcg agccgtgaac
    15421 tgctccatca acgcattgag cagcgttttc atcagatgtt ggcttcaggt tttgaagcag
    15481 aagtccgggc gctttttgcc cgaggagatt tgcatacgga cttgccttcc attcgttgcg
    15541 tgggttatcg ccagatgtgg tcttaccttg aaggcgaaat ctcatacgat gaaatggttt
    15601 atcgaggtgt ttgcgccacg agacagttgg cgaagcggca gataacctgg ctgcgtggtt
    15661 gggaaggggt tcactggctt gacagtgaaa aaccagaaca ggcgcgtgac gaagtattac
    15721 aggttgttgg tgctatcgca ggctgaatgt gtacaattga gacgtatcgt gcgcaatttt
    15781 ttcagaatcg aaaggttcaa agtacaaata agcatataag gaaaagagag aatggctaag
    15841 gggcaatctt tacaagatcc gttcctgaac gcactgcgtc gggaacgtgt tccagtttct
    15901 atttatttgg tgaatggtat taagctgcaa gggcaaatcg agtcttttga tcagttcgtg
    15961 atcctgttga aaaacacggt cagccagatg gtttacaagc acgcgatttc tactgttgtc
    16021 ccatctcgcc cggtttctca tcacagtaac aacgccggtg gcggtaccag cagtaactac
    16081 catcatggta gcagcgcgca gaatacttcc gcgcaacagg acagcgaaga aaccgaataa
    16141 ggtttcgggc tgttttttta cacggggagc cagcgatcct gcgttccccg ctgatctatt
    16201 tagagggtta tacgcttgtt tgaccgttat gatgctggtg agcaggcggt actggtacac
    16261 atctatttta cgcaagacaa agatatggaa gacctccagg agtttgaatc tctggtctct
    16321 tccgccggtg tcgaagcatt gcaggtgatt accggtagcc gtaaagcgcc gcacccaaag
    16381 tattttgtag gtgaaggtaa agcagttgaa attgcggaag ctgtcaaagc gatgggtgct
    16441 tcggtcgttc tttttgacca tgccctgagc ccggcgcaag agcgtaacct ggagcgtttg
    16501 tgcgagtgtc gtgttatcga ccgcaccggc cttattttag atattttcgc ccaacgtgcg
    16561 cgtacccatg agggtaagtt gcaggttgag ctggcgcagc tgcgccatct ggctacgcgc
    16621 ctggtgcgtg gctggaccca ccttgaaaga cagaaaggcg ggataggttt gcgtggtccg
    16681 ggtgaaaccc agctcgaaac cgaccgtcgt ttgttgcgta atcgcatcgt gcagatacag
    16741 tcgcgcctgg aaagagttga aaagcagcgt gagcaggggc ggcaatcgcg tatcaaagcc
    16801 gacgttccta ctgtttcgct ggtgggatat accaacgccg gtaaatctac ccttttcaat
    16861 cgcatcaccg aagcgcgggt ctacgcggca gaccagttgt ttgccaccct cgacccgacg
    16921 ttgcggcgta ttgacgttgc agatgtcggt gaaaccgtac ttgcagatac cgtagggttt
    16981 attcgccacc tgccgcacga tctggtggcg gcatttaaag ccacgttaca agagacgcgg
    17041 caagccacat tactgctgca cgtcattgat gcggcggatg tgcgtgtaca agaaaacatc
    17101 gaagcggtga atacggttct tgaagagatc gacgctcacg agatcccaac cctgctggtg
    17161 atgaacaaga tcgatatgct ggaagatttc gaaccgcgta ttgatcggga cgaagagaac
    17221 aaaccgatcc gtgtctggct ttccgcacag accggagcgg ggataccaca gctttttcag
    17281 gctttgacgg agcggctttc cggcgaggtg gcgcagcata cattgcgtct gccaccgcag
    17341 gaagggcgtc tgaggagtcg tttttatcag cttcaggcaa tagaaaaaga gtggatggag
    17401 gaggacggca gcgtaagtct gcaagttcgt atgccgatcg ttgactggcg tcgcctctgt
    17461 aaacaagaac cggcgttgat cgattacctg atctaacggc gtagcgtctg aagcgtggag
    17521 tcatatcctc tggcgtcgaa agacaacagg gatcaccgca taacaaatat ggagcacaaa
    17581 catggcgtgg aatcagcccg gtaataacgg acaagaccgc gacccgtggg gaagcagcaa
    17641 acctggcggc aactctgagg gaaatggaaa caaaggcggt cgcgatcaag ggccacctga
    17701 tttagatgat atcttccgca aactgagcaa aaagctcggt ggtctgggcg gcggtaaagg
    17761 caccggatct ggcggtggca gttcatcgca aggcccgcgc ccgcagcttg gcggtcgtgt
    17821 cgttaccatc gcagcggcag cgattgtcat tatctgggcg gccagtggtt tctataccat
    17881 taaagaagcc gaacgcggcg tggtaacacg ctttggtaaa ttcagccatc tggttgagcc
    17941 gggtctgaac tggaaaccga cgtttatcga cgaagtcaaa ccggtgaacg tggaagccgt
    18001 gcgtgaactg gccgcttctg gtgtgatgct gacgtcggac gagaacgtag tgcgcgttga
    18061 gatgaacgtg cagtaccgcg tcaccaatcc ggaaaaatat ctgtatagcg tgaccagccc
    18121 ggatgacagc ctgcgtcagg ctaccgacag cgccctgcgt ggagttatcg gtaaatacac
    18181 catggaccgc attctgacgg aaggtcgtac cgtgattcgt agcgatactc agcgcgaact
    18241 ggaagagacg attcgtccgt atgacatggg tatcacgctg ctggacgtca acttccaggc
    18301 tgctcgtccg ccggaagaag taaaagcggc gtttgacgat gcgattgccg cgcgtgaaaa
    18361 cgaacagcaa tacattcgtg aagcagaagc gtattccaac gaagttcagc cgcgtgcgaa
    18421 cggtcaggcg caacgtatcc tcgaagaggc gcgtgcgtac aaggcccaga ccatcctgga
    18481 agctcagggt gaagtggcgc gctttgctaa acttctgccg gaatataaag ccgcgccgga
    18541 aattactcgc gagcgtctgt atatcgagac gatggaaaaa gtgttgggta acacccgcaa
    18601 agtgctggtt aacgataaag gtggcaacct gatggttctg ccgttagacc agatgctgaa
    18661 aggtggtaac gcccctgcgg cgaagagcga taacggtgcc agcaatctgc tgcgtctgcc
    18721 gccagcctct tcctccacaa ccagtggagc aagcaacacg tcgtccacca gtcagggcga
    18781 tattatggac caacgccgcg ccaacgcgca gcgtaacgac taccagcgtc agggggaata
    18841 acgatgcgta agtcagttat cgcgattatc atcatcgtgc tggtagtgct ttacatgtct
    18901 gtctttgtcg tcaaagaagg tgagcgcggt attacgctgc gttttggtaa ggtactgcgt
    18961 gacgatgaca acaaaccttt ggtttatgag cctggtctgc atttcaagat accgttcatt
    19021 gaaacggtga aaatgctcga cgcacgtatt cagaccatgg acaaccaggc cgaccgcttt
    19081 gtgaccaaag agaagaaaga cctgatcgtc gactcttaca tcaaatggcg catcagcgat
    19141 ttcagccgtt actacctggc aacgggtggt ggcgacattt cgcaagcgga agtgctgttg
    19201 aaacgtaagt tctctgaccg tctgcgttct gaaattggtc gcctggacgt gaaagatatc
    19261 gtcaccgatt cccgtggtcg tctgaccctc gaagtacgtg acgcgctgaa ctccggttct
    19321 gcgggtacag aagatgaagt tactaccccg gcagcagata acgccattgc cgaagcggca
    19381 gagcgcgtaa cggctgagac gaagggcaaa gttccggtca tcaacccgaa cagtatggcg
    19441 gcgctgggta ttgaagttgt cgatgtgcgt atcaagcaga tcaacctgcc gaccgaagtg
    19501 tctgaagcga tctacaaccg tatgcgcgcc gagcgtgaag cggtagcgcg tcgtcaccgt
    19561 tcacaaggtc aggaagaagc ggaaaaactg cgcgcgactg ccgactatga agtgaccaga
    19621 acgctggcag aagctgagcg tcagggccgc attatgcgtg gtgaaggcga tgccgaagca
    19681 gccaaactgt ttgctgatgc attcagtaaa gatccggact tctacgcatt catccgtagc
    19741 ctgcgtgctt atgagaacag cttctctggc aatcaggacg tgatggtcat gagcccggat
    19801 agcgatttct tccgctacat gaagacgccg acttccgcaa cgcgttaata taacgactgc
    19861 ggtacaggtc aataaagcca ccgcatcctc agggatgtcg gtggttttct ttttctataa
    19921 ggataatgaa tgaattcgac aatctggctg gcgcttgccc tggttttggt actggaaggt
    19981 ttagggccga tgctttaccc gaaggcatgg aagaagatga tctctgcgat gaccaatttg
    20041 cccgataata ttttacgtcg ttttggcggt ggacttgtgg ttgcgggcgt tgtggtctac
    20101 tacatgttga ggaaaacgat tggctgaaca aaaaacagac tgatcgaggt catttttgag
    20161 tgcaaaaagt gctgtaactc tgaaaaagcg atggtagaat ccatttttaa gcaaacggtg
    20221 attttgaaaa atgggtaaca acgtcgtcgt actgggcacc caatggggtg acgaaggtaa
    20281 aggtaagatc gtcgatcttc tgactgaacg ggctaaatat gttgtacgct accagggcgg
    20341 tcacaacgca ggccatactc tcgtaatcaa cggtgaaaaa accgttctcc atcttattcc
    20401 atcaggtatt ctccgcgaga atgtaaccag catcatcggt aacggtgttg tgctgtctcc
    20461 ggccgcgctg atgaaagaga tgaaagaact ggaagaccgt ggcatccccg ttcgtgagcg
    20521 tctgctgctg tctgaagcat gtccgctgat ccttgattat cacgttgctc tggataacgc
    20581 gcgtgagaaa gcgcgtggcg cgaaagcgat cggcaccacc ggtcgtggta tcgggcctgc
    20641 ttatgaagat aaagtggcac gtcgcggtct gcgtgttggc gaccttttcg acaaagaaac
    20701 cttcgctgaa aaactgaaag aagtgatgga atatcacaac ttccagttgg ttaactacta
    20761 caaagctgaa gcggttgatt accagaaagt tctggatgat acgatggctg ttgccgacat
    20821 cctgacttct atggtggttg acgtttctga cctgctcgac caggcgcgtc agcgtggcga
    20881 tttcgtcatg ttcgaaggtg cgcagggtac gctgctggat atcgaccacg gtacttatcc
    20941 gtacgtaact tcttccaaca ccactgctgg tggcgtggcg accggttccg gcctgggccc
    21001 gcgttatgtt gattacgttc tgggtatcct caaagcttac tcaactcgtg taggtgcagg
    21061 tcctttcccg gccgaactgt ttgatgaaac tggcgagttc ctctgcaagc agggtaacga
    21121 attcggtgca actacgggtc gtcgtcgtcg taccggctgg ctggacaccg ttgccgttcg
    21181 tcgtgcggta cagctgaact ccctgtctgg cttctgcctg actaaactgg acgttctgga
    21241 tggcctgaaa gaggttaaac tctgcgtggc ttaccgtatg ccggatggtc gcgaagtgac
    21301 taccactccg ctggcagctg acgactggaa aggtgtagag ccgatttacg aaaccatgcc
    21361 gggctggtct gaatccactt tcggcgtgaa agatcgtagc ggcctgccgc aggcggcgct
    21421 gaactacatc aagcgtattg aagagctgac cggtgtgccg atcgatatca tctctaccgg
    21481 tccggatcgt actgaaacca tgattctgcg cgacccgttc gacgcgtaat tctggtacgc
    21541 ctggcagata ttttgcctgc cgggctaaca gtgtgataca ttgctgtgtc ggttaagcca
    21601 ttacgctatc cgacacagtg ttaaatcctc gcttttttcc ttccccgaac tgaaataaat
    21661 tagcgacaca gcttgtggct ggtttatcat caatataaat gtattttttc ccgatttccc
    21721 ttttgaggtt gatgtgcagt taacgagttt cactgattac ggattacgtg cgctgatcta
    21781 catggcgtca ttgccagaag ggcggatgac cagtatttct gaagtgactg acgtctacgg
    21841 cgtctcccgt aatcatatgg tcaaaataat caatcaactt agtcgtgccg gctacgtgac
    21901 tgctgttcgt ggaaaaaatg gcggcattcg cctgggtaaa ccggcgagtg cgatacgtat
    21961 tggtgatgtg gtgcgcgagc tggagccctt atcgctggtg aattgcagca gtgagttttg
    22021 ccacattaca cctgcctgtc ggttgaaaca ggcgctttct aaggccgtgc aaagttttct
    22081 tacggaactg gataactaca cgcttgccga tttggttgaa gagaatcaac cgctttataa
    22141 attattgctg gtggagtgac gaaaatcttc atcagagatg acaacggagg aaccgagatg
    22201 tcacaagatc ctttccagga acgcgaagct gaaaaatacg cgaatcccat ccctagtcgg
    22261 gaatttatcc tcgaacattt aaccaaacgt gaaaaaccgg ccagccgtga tgagctggcg
    22321 gtagaactgc acattgaagg cgaagagcag cttgaaggcc tgcgtcgccg cctgcgcgcg
    22381 atggagcgcg atggtcaact ggtcttcact cgtcgtcagt gctatgcgct gccggaacgc
    22441 ctcgacctgg tgaaaggtac cgttattggt caccgtgatg gctacggctt tctgcgggtt
    22501 gaagggcgta aagatgattt gtatctctcc agcgagcaga tgaaaacctg cattcatggc
    22561 gatcaggtgc tggcgcagcc gctgggtgct gaccgtaaag gtcgtcgtga agcgcgtatt
    22621 gtccgcgtac tggtgccaaa aaccagccag attgttggtc gctactttac cgaagcgggc
    22681 gtcggctttg tggttcctga cgatagccgt ctgagcttcg atatcttaat cccgcccgat
    22741 cagatcatgg gcgcgcggat gggctttgtg gtcgtagtcg aactgactca gcgtccgact
    22801 cgccgcacca aagcggtggg taaaatcgtc gaagtgctgg gcgacaatat gggcaccggc
    22861 atggcggttg atatcgctct gcgtacccat gaaattccgt atatctggcc gcaggctgtt
    22921 gagcaacagg ttgcggggct gaaagaagaa gtgccggaag aagcaaaagc gggccgtgtc
    22981 gatttgcgcg atttaccgct ggtcaccatt gatggcgaag acgcccgtga ctttgacgat
    23041 gcagtttact gcgagaaaaa acgcggcggc ggctggcgtt tatgggtcgc gattgccgac
    23101 gtcagctact atgtgcgtcc gccaacgccg ctggacagag aagcgcgtaa ccgtggcacg
    23161 tcggtgtact tcccttcgca ggttatcccg atgctgccgg aagtgctctc taacggcctg
    23221 tgttcgctca acccgcaggt agaccgcctg tgtatggtgt gcgagatgac ggtttcgtcg
    23281 aaaggccgcc tgacgggcta caaattctac gaagcggtga tgagctctca cgcgcgtctg
    23341 acctacacca aagtctggca tattctgcag ggcgatcagg atctgcgcga gcagtacgcc
    23401 ccgctggtta agcatctcga agagttgcat aacctctata aagtgctgga taaagcccgt
    23461 gaagaacgcg gtgggatctc atttgagagc gaagaagcga agttcatttt caacgctgaa
    23521 cgccgtattg aacgtatcga acagacccag cgtaacgacg cgcacaaatt aattgaagag
    23581 tgcatgattc tggcgaatat ctcggcggcg cgtttcgttg agaaagcgaa agaaccggca
    23641 ctgttccgta ttcacgacaa gccgagcacc gaagcgatta cctctttccg ttcagtgctg
    23701 gcggagctgg ggctggagct gccgggtggt aacaagccgg aaccgcgtga ctacgcggaa
    23761 ctgctggagt cggttgccga ccgtcctgat gcagaaatgc tgcaaaccat gctgctacgc
    23821 tcgatgaaac aggcgattta cgatccagaa aaccgtggtc acttcggtct ggcattgcag
    23881 tcctatgcgc actttacttc gccgattcgt cgttatcctg acctgacgct gcaccgcgcc
    23941 attaaatatc tgctggcgaa agagcagggg catcagggca acaccactga aaccggcggc
    24001 taccattatt cgatggaaga gatgttgcaa ctgggtcagc actgttcgat ggcggaacgt
    24061 cgtgccgacg aagcaacgcg cgatgtcgct gactggctga agtgtgactt catgctcgac
    24121 caggtaggta acgtctttaa aggcgtaatt tccagcgtca ctggctttgg cttcttcgtc
    24181 cgtctggacg acttgttcat tgatggtctg gtccatgtct cttcgctgga caatgactac
    24241 tatcgctttg accaggtagg gcaacgcctg atgggggaat ccagcggcca gacttatcgc
    24301 ctgggcgatc gcgtggaagt tcgcgtcgaa gcggttaata tggacgagcg caaaatcgac
    24361 tttagtctga tctccagtga acgcgcaccg cgcaacgtcg gtaaaacggc gcgcgagaaa
    24421 gcgaaaaaag gcgatgcagg caaaaaaggc ggcaagcgtt gtcaggtcgg taaaaaggta
    24481 aactttgagc cagacagcgc cttccgcggt gagaaaaaaa cgaagccgaa agcggcgaag
    24541 aaagacgcga gaaaagcgaa aaagccatcg gcgaaaacgc agaaaatagc cgcagcgacc
    24601 aaagcgaagc gtgcggcgaa gaaaaaagtg gcagagtgat caataccctc tttaaaagaa
    24661 gagggttaga ttgctgacaa aatgcgcttt gttcatgccg gatgcggcgt gaacgcctta
    24721 tccggcctac ataatcacgc aaattcaata tattgcagag atcatgtagg ccggataaac
    24781 gtagtgcatc aggcaaaacg taaacaacga gtacattaat gagcgaaatg atttacggca
    24841 tccacgcagt gcaggccctg ctggagcgcg cccctgaacg ttttcaggaa gtctttattt
    24901 taaaaggccg tgaagataaa cgtctgttac cgctgattca cgcccttgag tctcagggcg
    24961 tggttatcca gttggcaaac cgccaatatc tcgacgagaa aagcgacggt gccgtgcatc
    25021 agggcattat cgcccgcgtg aagccaggac gtcagtatca ggaaaacgat ctgccggatc
    25081 tgatcgcttc gctcgatcaa ccgttcctgc tgatcctcga cggcgtaacc gatccgcaca
    25141 acctcggcgc gtgcctgcgt agtgcggacg ccgcaggcgt tcatgcggtg attgtgccga
    25201 aagatcgctc cgcacagctc aacgctacgg cgaaaaaagt agcctgcggc gcggcagaaa
    25261 gcgttccgct gattcgggtg actaaccttg cgcgcaccat gcgtatgttg caggaagaga
    25321 atatctggat cgtcggtacg gcaggcgagg cggatcatac actctatcag agcaaaatga
    25381 ccggacgcct ggcgctggtg atgggcgcgg aaggtgaagg tatgcgtcgc ctgacccgtg
    25441 aacattgcga tgagttgatc agcatcccaa tggcaggaag tgtttcttcc ctgaacgttt
    25501 cggttgcaac cggaatttgc ttatttgaag cggttcgtca gcgcagctag ttcctctgaa
    25561 ttatgaaaag ccatccagat ttggatggtt tt
//
LOCUS       DAZOYF010000046        24809 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZOYF010000046 DAZOYF010000000
VERSION     DAZOYF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.046"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..216)
                     /locus_tag="ACHUOF_001769"
     CDS             complement(<1..216)
                     /locus_tag="ACHUOF_001769"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610978.1"
                     /translation="MPEQVITKAPYIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEI"
     gene            153..1652
                     /gene="pitB"
                     /locus_tag="ACHUOF_001770"
     CDS             153..1652
                     /gene="pitB"
                     /locus_tag="ACHUOF_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR0610979.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1701..2393)
                     /locus_tag="ACHUOF_001771"
     CDS             complement(1701..2393)
                     /locus_tag="ACHUOF_001771"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0610980.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2567..3280
                     /locus_tag="ACHUOF_001772"
     CDS             2567..3280
                     /locus_tag="ACHUOF_001772"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0610981.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3312..4070
                     /locus_tag="ACHUOF_001773"
     CDS             3312..4070
                     /locus_tag="ACHUOF_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610982.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            4116..5465
                     /locus_tag="ACHUOF_001774"
     CDS             4116..5465
                     /locus_tag="ACHUOF_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR0610983.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5465..6289
                     /locus_tag="ACHUOF_001775"
     CDS             5465..6289
                     /locus_tag="ACHUOF_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0610984.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            6301..6861
                     /locus_tag="ACHUOF_001776"
     CDS             6301..6861
                     /locus_tag="ACHUOF_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR0610985.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6892..7962
                     /gene="lptF"
                     /locus_tag="ACHUOF_001777"
     CDS             6892..7962
                     /gene="lptF"
                     /locus_tag="ACHUOF_001777"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR0610986.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            7959..9038
                     /gene="lptG"
                     /locus_tag="ACHUOF_001778"
     CDS             7959..9038
                     /gene="lptG"
                     /locus_tag="ACHUOF_001778"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR0610987.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9074..10245)
                     /locus_tag="ACHUOF_001779"
                     /pseudo
     CDS             complement(9074..10245)
                     /locus_tag="ACHUOF_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(10245..10493)
                     /locus_tag="ACHUOF_001780"
     CDS             complement(10245..10493)
                     /locus_tag="ACHUOF_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR0610988.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10526..11440)
                     /locus_tag="ACHUOF_001781"
     CDS             complement(10526..11440)
                     /locus_tag="ACHUOF_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR0610989.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11437..13125)
                     /locus_tag="ACHUOF_001782"
     CDS             complement(11437..13125)
                     /locus_tag="ACHUOF_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFR0610990.1"
                     /translation="MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
     gene            13529..14670
                     /gene="yghO"
                     /locus_tag="ACHUOF_001783"
                     /pseudo
     CDS             13529..14670
                     /gene="yghO"
                     /locus_tag="ACHUOF_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
     gene            complement(14794..15564)
                     /gene="glcC"
                     /locus_tag="ACHUOF_001784"
     CDS             complement(14794..15564)
                     /gene="glcC"
                     /locus_tag="ACHUOF_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFR0610991.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            15815..17314
                     /gene="glcD"
                     /locus_tag="ACHUOF_001785"
                     /pseudo
     CDS             15815..17314
                     /gene="glcD"
                     /locus_tag="ACHUOF_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            17314..18366
                     /gene="glcE"
                     /locus_tag="ACHUOF_001786"
     CDS             17314..18366
                     /gene="glcE"
                     /locus_tag="ACHUOF_001786"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFR0610992.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            18377..19596
                     /gene="glcF"
                     /locus_tag="ACHUOF_001787"
                     /pseudo
     CDS             18377..19596
                     /gene="glcF"
                     /locus_tag="ACHUOF_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            19601..20005
                     /locus_tag="ACHUOF_001788"
     CDS             19601..20005
                     /locus_tag="ACHUOF_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFR0610993.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            20027..22198
                     /gene="glcB"
                     /locus_tag="ACHUOF_001789"
     CDS             20027..22198
                     /gene="glcB"
                     /locus_tag="ACHUOF_001789"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFR0610994.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFFRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            22553..24139
                     /locus_tag="ACHUOF_001790"
     CDS             22553..24139
                     /locus_tag="ACHUOF_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HFR0610995.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         5942 a         6054 c         6610 g         6203 t
ORIGIN      
        1 tatttctaaa ggttttgaat gaatgcttat tgtctgatac acaagaaata acactctttt
       61 tatcgttaaa aaatgatatt tcactttgcc catgccgtta aagtcactga taatgcgtcc
      121 gttcgtaaat tcaaaatggc gtaatctaat atatgctaaa tttatttgtt ggccttgata
      181 tatatacggg gcttttgtta ttacttgctc tggcatttgt gttgttctac gaagcaatca
      241 atggttttca tgacacggcg aatgcggtgg caaccgttat ttatactcgt gccatgcagc
      301 cacaacttgc tgtggtgatg gcggcatttt ttaacttttt tggcgtgtta ttgggcggac
      361 ttagcgttgc ctatgccatt gtccatatgt taccaatcga tttgttgctg aatatggggt
      421 caactcacgg tttggcgatg gtcttttcca tgctgctggc ggcgattatc tggaacctgg
      481 gaacgtggtt ctttggttta ccggcctcca gttcgcacac cttgattggt gcgattatcg
      541 gcatcggttt aaccaacgcg ctgttaaccg gctcatcggt gatggatgcg ttaaacctgc
      601 gtgaagtaac caaaattttc tcctcgctga ttgtttcccc tatcgtcggc ctggtcattg
      661 cgggtggtct gattttcctg ctgcgccgtt tctggagtgg gacgaaaaag cgtgaccgta
      721 ttcaccgcat tccggaagat cgcaaaaaga aaaagggcaa acgtaaaccg ccattctgga
      781 cgcgtattgc gctgattgtt tccgctgcgg gcgtggcgtt ttcgcacggc gcgaacgacg
      841 ggcagaaagg gatcggcctg gtaatgctgg tactggtggg gattgcccct gctggcttcg
      901 tcgtcaatat gaatgcgtcc ggctatgaaa ttacccgtac ccgcgatgcc gttaccaact
      961 tcgaacacta cctgcaacag catcctgaac tgccgcagaa gttgattgcg atggaacctc
     1021 cattgcctgc agcatcgact gatggcacgc aagtaacaga gtttcactgt catccggcaa
     1081 atacctttga tgcgattgcg cgcgttaaaa cgatgctgcc aggcaatatg gaaagttacg
     1141 agccgttaag cgtgagtcag cgcagccagc tgcgccgcat tatgctgtgc atctccgata
     1201 cctccgcgaa gctggcgaaa ctgccaggcg tcagtaaaga agaccagaac ctgctgaaaa
     1261 aactgcgcag cgatatgtta agcaccattg agtacgctcc ggtgtggatc atcatggcgg
     1321 tagcactggc gctcggcatt ggcaccatga ttggctggcg tcgtgtagcg atgaccatcg
     1381 gtgagaagat tggtaagcgc ggcatgacgt atgcgcaagg catggcggca caaatgacgg
     1441 cggcagtgtc tatcggcctt gccagttata ttgggatgcc tgtctccaca acacacgtcc
     1501 tctcgtctgc ggtagcaggg acgatggtgg tggacggcgg tgggttacag cgtaaaacgg
     1561 taaccagcat cctgatggcg tgggtgttta ctttaccggc ggcaattttt ctttctggtg
     1621 ggctgtactg gatagcattg cagttgattt aaccttcctg aaaatgcccg gtcctggcga
     1681 tcgggcattt ccatttttga ctagtgataa ccacgcgcgg tcataaaatc cgtaatcgct
     1741 ttttctgcat caaccaacac ctgttccagt ggctgattgg catcgatatc aaccagttgt
     1801 gcaccaccaa aggttaactg tggcgttatg gcaatcttcc tcgccagcga ttcccgttta
     1861 tggtcgggtt tacgtgcaca ggcaacttca aggtcaacat tgagtttgat gaccagatca
     1921 ggcttatggc tcgccatcca gtgaaacgct ttacgttcct ggcttgccag ccatgagaca
     1981 aaacgaccac cttcaacgtt aggtgggaac accgtaccat cgtaagcgcc aggaatttgg
     2041 tcctgaggat aacggtcggt tagaacaatt aacccgcgac gacgacaggc aagcatatga
     2101 cgaaagcgca gtaagcgacg ggcgacaaac gctgtaatta ccagcgccgg aactggtcca
     2161 ggcaattttt ttgctgtttt cacctgattt cgttcaattg ttttatgtaa ggattttccc
     2221 atcaaaggta attttgtcac tgcacgaccg acatttccgg cctgttttcc taaatgaact
     2281 ctttcggcag caccatattt ttcgacaacg gtaataagat gttcacacac cgttgacttg
     2341 cctgaagcat cgctaccaat aacggcaatt aatggaggtg ttattgactg catgaatata
     2401 tcctttaatt taaatatcca ttaaaaatat ttatttggtt aatatgtttt tatgaaagcg
     2461 taattcaggt caatgtcaca attagtcatt gtcacaataa ggttgaacgg atattctgag
     2521 cacggaccac gtaatattca atacattatt tactgccggg tttttcgtga atcaacgtaa
     2581 tatgtcaata attaattcca cgccagtgcg tgttattgcc attgtaggat gtgatggttc
     2641 aggtaaatcg accctcacgg caagcctggt aaatgaactg gcagcaagaa tgccaacaga
     2701 acacatttat ctcgggcaat cgtccgggcg aattggcgaa tggatttcac agctccctgt
     2761 tattggcgca ccttgtgggt gttatctgcg aagtaaagcg gcacatgtac acgaaaagcc
     2821 cttaactccg cctggcaata ttactgcgct ggttatctat ctgctttcct gctggcgggc
     2881 gtacaagttt cgcaaaatgt tgtgtaaaag ccagcaaggc tatctgctca tcaccgaccg
     2941 ctacccgcag gttgaagtgc cgggttttcg ctttgatggc ccgcaattgg caaaaaccac
     3001 gggcggtaac ggttggataa aaatgttaag gcagcgcgag ttgaagctgt accaatggat
     3061 ggcgtcttat ttacccgtat tgttgattcg tcttggcatt gatgaacaaa ccgcgtttgc
     3121 gcgtaaacct gaccaccaac tggcagcgtt acaggaaaaa atcgccgtta cgccgcaact
     3181 gacattcaat ggcgccaaga tccttgaact ggatggtcgg caaccagccg atgaaattat
     3241 gcaagcgtca ctacgcgcaa ttcacgccgc gctttcctga tcaccaaaat ggatagggga
     3301 tgatttaacg aatggatgca ctacaaacac aaactgttaa tagtacaacc gcgccgcagc
     3361 ccaactacat tccggggctg attgcggtgg tcgggtgtga tggcaccggt aaatccacgc
     3421 tgaccaccga cctggtgaaa tcgctgcaac aacactggca aaccgagcgc cgctatctgg
     3481 ggctgctctc cggcgaagac ggcgacaaaa tcaaacgatt gccgttggtt ggcgtctggc
     3541 tggaacggcg attggcggcc aaatcctcga aaacccaaag catgaaaacc aaatctccgg
     3601 cgctatgggc ggcggtgatt atgtactgct tctcgctgcg aagaatggcg aatctacgca
     3661 aggttcagcg actggcgcaa agtggcgttc tggtggtcag cgatcgcttc ccgcaggctg
     3721 aaatttcggg cttttattat gacggaccgg ggatcggcgt cgaacgtgcg accgggaaaa
     3781 tcagcatgtt tctggcgcag cgcgaacgga gtttatacca aaaaatggcg caataccgtc
     3841 ctgaattaat cattcgtttg gggattgata ttgatactgc catctcccgc aagcctgacc
     3901 atgagtatgc cgagctgcag gacaaaatcg gcgtcatgtc gacgattggc tataacggca
     3961 caaaaattct tgagattgat tccagagcgc cctacagcga agtgctggaa caggtacaga
     4021 aggcggtttc tctggttgcc atcgtttctg accgccgaag tttaacttaa ggctggcggg
     4081 atatttttta ctatcaacga taaggtcagg ctgaattggc gggttttaac atcaaacatt
     4141 ggtttgcaga tggcgcgttt cgcaccatta ttcgcaatag cgcctggtta ggctccagta
     4201 atgtcgagag cgccttgctg gggctgttgg cgctctcgtg tgccggtaaa gggatgacgc
     4261 ccgccatgtt tggtgtactg gtgattgtgc aatcgtacgc caagtcgatc agcgatttta
     4321 ttaagtttca gacatggcag ctggtggttc agtacggaac gccagccttg accaacaata
     4381 atccgcagca atttcgcaat gtcgtctcat tttccttctc gctggatatc gtcagcggcg
     4441 cggtggcgat tgtcggcggc attgccttac tgccattcct ttcccattca ttaggtctgg
     4501 atgaccaaag cttttggctg gcggcgctgt attgcacgct cattccttca atggcttcct
     4561 ccacgccgac cggcattctg cgtgcggtag atcgctttga tttgattgct gtacagcagg
     4621 cgacgaaacc ttttctgcgc gcagcgggga gcgtcgcagc ctggtatttt gactttggtt
     4681 ttgtgggttt tgttattgcc tggtacgtgt cgaatctggt tggcggcacc atgtactggt
     4741 ggtttgccgc gcgcgaatta cgccgtagaa atatccataa cgccttcaaa ttgaatctgt
     4801 ttgagtctgc ccgacacatt aaaggcgcgt ggagttttgt ctggtcaacc aacattgccc
     4861 actccatctg gtcggcgcgt aactcgtgca gcacagtgtt ggtggggatc gtgttaggac
     4921 ccgctgccgc cgggttattt aaaatcgcca tgacattctt cgacgccgcc ggaacgccag
     4981 cgggtttgct gggtaaaagt ttttacccag aggtgatgcg tttagatccg cgcaccacca
     5041 gaccgtggtt gttaggtgtg aagtccggtt tactggcggg cggaatcggt attctggtgg
     5101 cgctcgcggt gttgatcgtc ggcaagccgc tcatttcgct ggtgtttggc gttaagtatc
     5161 tcgaagcgta tgacctgatt caggtgatgc tgggcgcaat tgtgatttcg atgctgggct
     5221 tcccgcagga gtcattgcta ttgatggcgg gaaaacagcg cgcttttctc gtggcgcaaa
     5281 ccatcgcctc aatcggctac atcgtattgc tgttcatgtt ctgtcatctg tttggtgtgc
     5341 tgggagcagc gtttgcttac ttcggtgggc aatgtctgga cgtggcgctc tcgttaattc
     5401 caaccttaaa ggcatttttt cagcgccatt ccttgcttta taacgcagcc ggagagaaat
     5461 cctgatgatg agaaaatatt tcccgcttga ggcgagcgag cgtctatttg ttgcgattga
     5521 agaagatgat gtcgttgatg cacaggttag tttgccgccg actattgcac taagttgtac
     5581 caccgaaatc attcatgata attacgcgtt atgcctgcaa ttctggctca atggcgttga
     5641 tcgtcaggaa ttactgcgtt tagttcgcaa acaagccaaa ggcgatgagc tgactgctga
     5701 tgagcgcaaa caatttaaat acatgcgagc gcgctacaag cacctgcgtt ttgcgcaacg
     5761 gctgtattta aagaagcatc aggccggatt tttattcggc aaaacgaccg tttttctggg
     5821 gcgttttcag gacggctttc gtaacggtaa gaaaaatatt gtgtcgtatt acggcaacct
     5881 gttacggatt tatttaagct ctccggtctg gtcgttggtg aactattcat tacgccatag
     5941 ccagttagaa agcgtaagca gttttattgc ctatcggcaa aagcaaatgc ataccctgaa
     6001 agagattatt gccaaaccgc gactaaccgg cagggaattt catgatgtgc gaaaaatcat
     6061 cagccagcag gtttcttatt acgacacctt aagatcgctt gatcctgaaa ataaagaggc
     6121 gttacagatt tcccgtttcc tcgccgccat taatggcctg atgggagata aacacgacga
     6181 catggtggcg gatgatatgg aaaaccgcca gtcgtattat gcaccgttgg cgctggatag
     6241 cgatatttgt cagcgcttag agctgctgat ttcgcgtttt ccgctatagc cggagcaatg
     6301 atgtcatctc gtttgattat tgcgttgatt atcatgctct tagccccggg tgtgcaggca
     6361 cacaatttcg tcactggcaa aacggttacc cctgtttata tccaggaagg cggcgagctg
     6421 cttctgaata gcgacgacga aattcactac caaaagtgga acagcacaca attagcggga
     6481 aaagtccgca ttattcagta tatcgccggg cgtaagtcgg cgaagaaaaa gaactcactg
     6541 ttaattaaag cggttgaagc ggcaaacttt ccgcaggatc gttttcagcc caccaccatt
     6601 gtgaacaccg atgatgccat ctttggtact ggctattttg ttgttggcaa aatcgaaaaa
     6661 aataaacgcc gctatccgtg ggcgcaattc gttattgacg gcaatggaca gggacgcgtt
     6721 gcctggcgct tgccggagca gagttcaacc attctggtgc tgaataaagc cgggcaaatt
     6781 cagtgggcaa aggacggcag cctgacgccg gaagaggtag atcatgtcat tgcgttggcg
     6841 cagaaactga tcaatgaata aaaaactcaa aggattgagg gcgttagtga gatgaagctg
     6901 gttgaacact acatcatgcg tggtacgcgc cgtctggtgc tgattatcgt cggttttctg
     6961 atctttattt tcgccagcta ctccgcacag cgttatctga ccgaagcggc aaatgggaca
     7021 ttagctcttg atgttgtgct ggatatcgtt ttctacaaag tactgattgc actggagatg
     7081 ttgttacctg ttggtctgta tgtgtcagtt ggcgtgacgc tagggcagat gtataccgac
     7141 tcggaaatta ccgctatctc tgcggcgggg ggcagtccgg gacgcttgta caaagccgtt
     7201 ctttatctgg cgataccgct aagtattttt gtcacccttc tgtcgatgta tggtcgtccg
     7261 tgggcttatg cgcagattta tcaactggag cagcagtcgc agtcggagct ggatgttcgc
     7321 cagttgcggg caaagaaatt taacactaac gataacggac gaatgatcct ttcgcagacg
     7381 gttgatcagg ataataatcg cctgactgac gcgctgattt atacttctac tgccaatcga
     7441 acccgtattt tccgcgccgg ttcggttgat gtggttgacc catcacccga gaaaccgacc
     7501 gttatgttgc acaacggcac tgcctatctt ctcgatcatc aggggcgtga cgacaacgaa
     7561 cagatctacc gtaatctgca attacatctg aatccgctgg atcaaagccc gaacgtcaaa
     7621 cgcaaagcga aatcggtcac ggagctggcg cgctccgcct ttcctgccga tcatgccgaa
     7681 ctgcaatggc gacaaagccg tggcctgacc gcattgttga tggcgctgct ggccatttca
     7741 ttaagtcggg taaaaccgcg gcaagggcga ttttcaacgt tattgccact gacgttgctg
     7801 tttattgcca ttttttatgg cggcgacgtc tgccgtacac tggtggctaa cggcgcgatt
     7861 cccctcattc ctggtttgtg gttagtgccc ggactcatgc taatgggcct gctgatactg
     7921 gtcgcacgtg acttctcttt gctgcagaaa ttttcccgat gaatgttttc agtcgctatt
     7981 taatccgtca tctctttctc ggttttgccg ccgctgcagg gctattgctg ccgcttttta
     8041 ccaccttcaa cctgattaac gaactggatg gtgtcagccc gggcggttat cgctggactc
     8101 aggcggtgct ggtggtgcta atgaccttac cgcgcacact ggtcgaactt tcgccgttta
     8161 tcgcgttatt gggtggtatt gttggactgg ggcagttatc gaaaaacagt gagcttaccg
     8221 ccattcgcag cacggggttt tctatcttcc gtattgcact ggtggcgctg gttgccggga
     8281 tattgtggac tgtttcgtta ggcgcgatag atgagtgggt ggcgtcgcca ttgcagcaac
     8341 aggcgttgca aatcaaatcg accgccaccg cgttggggga ggacgatgac attaccggca
     8401 atatgctgtg ggccaggcgt ggtaatgaat ttgtgacggt gaaatcgctg aacgagcagg
     8461 gccagccggt gggcgtggag atatttcatt atcgcgacga tctctcgctt gaatcctaca
     8521 tttatgcacg cagtgccacc attgaagacg acaaaacgtg ggtcctgcat ggtgtgaatc
     8581 ataaaaaatg gctgaatggc aaagaaacgc tggaaacatt agataatctt gcctggcaat
     8641 cggccttcac cagtatgaat cttgaagagt tatcaatgcc ggggaatact ttttctgtcc
     8701 gtcagcttaa tcattacatc cattatttgc aggaaaccgg gcaacccagc agcgaatacc
     8761 accttgcact gtgggaaaaa ctggggcaac ccatcctgac cctggcgatg attttgctgg
     8821 ctgtgccgtt tacctttagt gccccgcgtt cgcctgggat gggtagccgt ctcgctgtag
     8881 gcgtcatcgt gggcttactc acctggatca gctatcaaat catggttaat ctgggattgc
     8941 tatttgcgtt aagcgcacct gttaccgcgc tcggtttacc ggtagcgttt gtgttggtgg
     9001 cgttgagcct ggtgtattgg tatgacagac aacattaaac ccgccgtggc gcaaacacca
     9061 cggcgggctc acattactgc ttcagagtag caaatgcctg aatgatggca tcgatttgct
     9121 cacgactgtg ggcagcattt acgctacagc gcagcagcgt gatgcctgca ggcgcggcag
     9181 gcggcaggat aagattcaca tacacgcccg gcgagataag ctcgcgccag atgcgtaaac
     9241 cttcctcttt cgaaccgatt attactggca ctaccgggct aatatgtgaa cttaactcat
     9301 aacccagttt ttgcagaccg tcatacagat ggttggcgtt atccatcagc ttttgccgca
     9361 gctctggttg tgtggcaata gttcttaacg acgagcgcac ggtcgcaatg caggacggag
     9421 aaggagaggc ggtaaaaata tacgggcggc tgccgtagcg cagcacttcc atcgcttccg
     9481 aaacgacagc aaaaccgcca atcgaggcca gacttttact gaaagtacca acgataatat
     9541 ccacatcatc ttcaacgccg acagcttcag ccagtccgcg ccccgttgcg cctaacacgc
     9601 caaacgaatg cgcttcatcg acaatcaaat aaccaccgag acgacgtttg atatcaacaa
     9661 tttccgccag cggcgcaacg tcaccgagca tgctgtagat gccctcaacg atgataatcg
     9721 cctctttcgc ccgttcaccg aggcgaacca tgcgccgttc cagatcttta gcatcgttat
     9781 ggcggaaacg aataatttcc gcgccaccga gagaacagac gtcataaata ctggcgtggc
     9841 tgtcggcatc cagtaacacc acggcgttat ggtcggcaag tgcgctgatg acgcctaaat
     9901 tagcggtata accggttgaa aaacgatagc ggtagggcga ttgaaaaact cggcaatctc
     9961 tttctctaac gccaggtgcg gcgcataact gccgtttgcc atacgagaac cggtggtgcc
    10021 ggtgccttgg gccgctaacg cggcctgacc ttcgctgatg gcatcatgat taaaggttaa
    10081 accgagataa ttattggtcc cggccagaat cactttttta ttgccgatcc ggccttcggt
    10141 ggcggaatac acttcatcaa tacaggtgcc aaacggtgtt aacccgttgt cactaaattg
    10201 cagacgctcg ccagcaaggc gcgcatattt atcgtatagc cccattattt attctccagc
    10261 catggaagta gagtttccat taattgcgca ggggttttga catccagcag aatattaata
    10321 ggaatggaaa tatcgaatct gtcttccagc atcattaaga gatccatcac tttgatcgac
    10381 tccaggccaa gatcgttgac gagatcgctg tcgggcttaa tttccacacc gttttcgacc
    10441 aaatcctgta aacaggaaag gatgtaatcc attactattt cacgatttac catagaaacg
    10501 ttaacacacc tttaaaacaa gacaatcaaa atagcccgtt gcgtaatgcg tgttccaggc
    10561 taatcccggg aaaccaattg atatcttcag aaatacggtt attacttgcc gaccagtcgg
    10621 cgtgggttaa ttcacgaatt ttcgagcggg tcagcatagg ttctttacct gccaggcgac
    10681 tcaacgcggt actgatatcc gcaaggcctg tgagtaccgg cagaggaatg tcaaccattc
    10741 gcacggaacc acaacgggct ttggcggcaa gctgtcgtac gcgttgccag tcatagccac
    10801 cggcgacgcc atcgcataat tcataggttt gcgtctgtac agtttcggcg cttaaccact
    10861 gacccactgc ttgcgcaaaa tcggtgacgt gaagaaaaga gagttgtgta tctggcgcac
    10921 caagtcgtgg cagcaggccg cgtagcatcc agtcaaacag cggttttaac tctttatcgc
    10981 cgggaccata cacggctgtc gggcgaaaaa cgcccagcgt aatttcgtcc gccattgctg
    11041 ccagtcgttg ctcggcgacg tatttggatt ttgcatacca ggagagctcg ggatggcgcg
    11101 ccgccagcga agagataaac agaaaacgtt ggcaaaagcc gctctctttt gctgcttgca
    11161 tcaggcgcag gctgccgtca acgttacagc gggtgaaaat ctcttctttg tgcccgcgca
    11221 cttgcccggc gcaatggacc actacgctgg ctccggcaac cagctcgcta agtgaatgag
    11281 tatcttccag cgaaccgcgc acccaggtga gattatcatt gacgtgagcg cgggcagtac
    11341 gcgtcaatgc gcgaacgtga aagccgcggg cgagcaggtt atcgataata tatttaccga
    11401 taaacccggt agcgcccgtc accgcgacag tttgatttat gccagggatt cctgcacatt
    11461 aagactggca gcataagcct tctgataacg ttttttcgct tccgcacggg caggcttgcc
    11521 ggaggacgtt cggggaatac tgtggggcgg caacagatcg atagccgcgg tcacgccaaa
    11581 ttcgctttgg atccgtgccg ccagcgcgtg gataagctgc ccgcgacgtt cttcgtcgct
    11641 gatccgacac tggatctgca aaatgatttt ttcctgggcg gtaacaaaag caatcgcatc
    11701 gccagaatga atttccggtt cctgttccgc tatatattca atatcctgcg gccagatatt
    11761 acggccacga ataataatca gatctttaat gcgtccggtg acatacagat aaccgtccag
    11821 cagataaccg aggtcgccgg tgtctaacca gcccgtcgcg gcaatctcgt cttgcgaaac
    11881 ctggtcgcca aagtaaccgc tcatcagact gggaccggag atgcaaatat ggcctaccac
    11941 acgttccgcg accggcatac ctgcttcatt gcggatttca ataccatgtt ccggcaacgc
    12001 tttgccgcag ttgacgaaag tcgatacggc gcgtgtctct gcacccggcg cgacggcttt
    12061 accctgatat tcgaggatgt cgcgatccac ttcgttaacc acaaccccgg aggcttcatc
    12121 agagaagctg acagccagcg cattttctgc cagtccgtag cacggcatga aagttttatt
    12181 gtcaaagtta acctgacgga aacattcagc gaattgatgg agttgttctg cggagatcgg
    12241 ctcagcacca ataccagcga cgcgccagca ggaaagatcc agttcagcga gatctttttc
    12301 attcacgcgg cgttggcaca attcatagcc aaacggcggc gcaacggaaa cggtgccgcg
    12361 atttttactg atcaatttaa gccattgcaa aggacgcatg gcaaaatcct gagtgcgcaa
    12421 ataatctact gaaagttgcg tggcgacggg gggcaggaga aagccgacca gtcccatatc
    12481 atggtagaaa ggctgccagg agacgcagcg gtcgctaggg cgtaatttaa tgccatcgtg
    12541 gcttatagca cgtagattag ccatcacttc gcgatgggtg ataatgacgc cacggggaaa
    12601 acgggtgctg ccggaggtgt actggaggta ggcgatatcg ttcggaactg gacgctggag
    12661 cgcaacatcg gcttccggta atgccttaaa ccaagcgtgg cttaaaacat gtaattcggg
    12721 gttgttatgc gtcgcggcat tgaccagtgg caaccactca tcaccagtga taatggctgc
    12781 gggctggcaa cttgccagta aaccctggag tttggcgctc caggaatccc gctgaccgac
    12841 gcccattgga atcgccaacg ggacggcgac taagccagca tactgacagg caaaaaaagc
    12901 ctctacaaac ccgctactcg tttcggcaat cagcgccacg cgttcgcctt ttttcaggtt
    12961 cagcgataac aaccgcttcg caccagcttc ggcacgcgtt tttaatgtct gatattccag
    13021 ttgatcttca agttggcaac gtctgtcata aaaattcatt ccggctctac tcagagcggc
    13081 gtagtccaaa gcatcaacca gcgttggaaa atcggcatag cgcataggta gggaatgcgt
    13141 aaagatttta ttagacatat acaccacgaa aaaattggat atcaaaaata aaatgacaat
    13201 aatgcggtgg attatatagg cataaaagat aaatgagata gtattttcat cggtgtgaaa
    13261 atgaacattg atttaaataa tgagtttaag ttttttgttt ttattatgaa tgatatttaa
    13321 tttatgaata tattaagaag aagccttgtc cggtcgcctt aaattgacaa aaggcattga
    13381 ggcgtttctg tgtagcctct gaccttatca taatcattaa ttaatactat ttattcgtcg
    13441 tttgtattca ttgagttgat tattaagatg ccgctcgttg ctgttgcaac ctgtttttca
    13501 ccattaatta tttgttatcc acagagatat ggaatgcgat ctgctgatga ttaaaattga
    13561 aaaagtaatt aataaaaatg accttaaggc atttatcgct tttccgtcat cactttatcc
    13621 cgacgatcca aactggatac cccctttatt cattgaacgc aacgagcatt tgtctgcgaa
    13681 aaatccgggg acggaccata tcatctggca ggcgtgggtg gcaaaaaaag cggggcagat
    13741 agttgggcgt attaccgcgc aaatagatac cttgcaccgt gagcgttacg gtgaagatac
    13801 cggtcatttc ggcatgattg acgccattga tgatccgcag gattttgccg cgttgtttgg
    13861 cgcagcggaa gcgtggttga agtcacaagg tgcaagtaag atcagcgggc ctttcagcct
    13921 gaatatcaat caggaaagcg gattactgat tgaaggtttt gacacaccac cctgtgcgat
    13981 gatgccacac ggcaaaccgt ggtatgccgc gcatattgaa caactgggtt atcacaaagg
    14041 cattgattta ctggcgtggt ggatgcagcg aaccgatctc actttctctc cggcgctaaa
    14101 aaaactgatg gatcaggtgc gcaaaaaggt gaccattcgc tgcatcaatc gtcagcggtt
    14161 tgccgaagag atgcagatcc tgcgtgagat tttcaactct ggctggcagc acaactgggg
    14221 atttgtgccg tttaccgaac atgaattcgc gaccatgggc gaccaactta aatatctggt
    14281 gccggacgat atgatctaca tcgctgagat tgattctgca ccctgcgcgt ttattgtggc
    14341 ttgcctaaca tcaacgaggc gattgccgat ctgaacggat cgctctttcc cttcggctgg
    14401 gcaaaattgc tgtggcgttt gaaagtcagc ggtgtgcgaa ctgcgcgagt accgctgatg
    14461 ggcgtgcgcg acgagtatca gttcagccgc atcggcccgg tgattgcgct gttattgatt
    14521 gaagccttac gcgatccgtt tgcccgccgg aagatcgatg cgctggagat gtcatggatc
    14581 ctcgaaacca atacaggcat gaataacatg ctggaacgca ttggggcgga gccgtacaag
    14641 cgttaccgat tgtatgaaaa gcaaatttaa taccagatcc tgtttagcgc aggggcggtc
    14701 attcgggaga gggtatagat gaacagattt aacaccagat cctgttaagt gcaggggcgg
    14761 tcccctcgcc ttggggatag ggtaaaactc tgctcacatt cccaccttgt tcatctccag
    14821 cggcaccgcc gagcgaatca aatggtggcc ttccagctcg atatcgtgga gattcttttt
    14881 cacggtccgt acatgatcgc gcgctgcgcg ctgagcgacg tgcggcagcc gctgcaacac
    14941 cgcgttgtag atccgcgcat gctggcgatc gatctgcttt ttttgctgtg gtcgatggta
    15001 gagattattt acgctggcaa acactgaatt aaacatcaga tcggtcaatg attgcagcgt
    15061 aaacaccagc acctgattgt gagaagcctg acaaatggcg agatggaaag cgtgatccaa
    15121 ctgcgcatgt tcgatcagcg aaatctcttt gtggttctca ctggcggcga gcattttttc
    15181 ataacagcgg gttatcacaa caaaatcagc ctgcgttccc agcgttgccg ccagccttgc
    15241 cgattcgccc tccagtaatg cgcgaacgtc gagcagatcg tacagcgttc gcggctgcgt
    15301 actgaacaga tggatcagcg ggctggtgtc ctgctcccga ttaagccgtg cgacatgaga
    15361 atcgcgaccc tgcgccgttt caataatccc gcgcccgcgc agcacggtca gcccttcacg
    15421 cagtgcggag cgtgagaagc cgagcttttc acacagtcga cgttccgagg gaagcggctg
    15481 accgaccttc agtacgccgt cgataattaa ccgttcgata ctctctgcaa ccacttcgca
    15541 aatagggcga cgttcatctt tcattcccgg acctcgtgca caggtaggac caatttttct
    15601 gttcttaact cgcaaaacac gcacatcacg caagtgatta tgttacatca atttaacatt
    15661 aagttaacca agacaaggtc acagagctgg aaaaaaaatg gtctgaccgg tagctaaaga
    15721 gatagacgaa aacgaaaagc ccgcttaata actgttcaca gaagcagcgc gcaaaaatca
    15781 gctgccacac aacacaacaa agcgaagcct actcatgagc atcttgtacg aagagcgtct
    15841 tgatggcgct ttacccgatg tcgaccgcac atcggtactg atggcactgc gtgagcatgt
    15901 ccctggactt gagatcctgc ataccgatga ggagatcatt ccttacgagt gtgacgggtt
    15961 gagcgcgtat cgcacgcgtc cattactggt tgttctgcct aagcaaatgg aacaggtgac
    16021 agcgattctg gctgtctgcc atcgcctgcg tgtaccggtg gtgacccgtg gtgcaggcac
    16081 cgggctttct ggtggcgcgc tgccgctgga aaaaggtgtg ttgttggtga tggcgcgctt
    16141 taaagagagc ctcgacatta accccgttgg tcgccgcgcg cgcgtgcagc cgggcgtatg
    16201 taacctggcg atctcctagg ccgttgcgcc gcataatctc tattacgcgc cggatccttc
    16261 ctcacaaatc gcctgttcaa ttggcggcaa tgtggcggaa aatgccggtg gcgtccactg
    16321 cctgaaatat ggtctgaccg tacataacct gctgaaaatt gaagtgcaaa cgctggacgg
    16381 cgaggcgctg acgctgggat cggacgcgct ggattcacct ggttttgacc tgctggcgct
    16441 gttcaccgga tcggaaggta tgctcggcgt gaccaccgaa gtgacggtaa aactgctgcc
    16501 gaagccgccc gtggcgcggg ttctgttagc cagctttgac tcggtagaaa aagccggact
    16561 tgcggttggt gacatcatcg ccaatggcat tatccccggc gggctggaga tgatggataa
    16621 cctgtcgatc cgcgcggcgg aagattttat tcatgccggt tatcccgtcg acgccgaagc
    16681 gattttgtta tgcgaactgg acggcgtgga gtctgacgta caggaagact gcgagcgggt
    16741 taactacatc ttgttgaaag cgggcgcgac tgacgtccgt ttggcacagg acgaagcaga
    16801 gcgcgtacgt ttctgggccg gtcgcaaaaa tgcgttcccg gcggtaggac gtatctcccc
    16861 ggattactac tgcatggatg gcaccatccc gcgtcgcgcc ctgcctggcg tactggaagg
    16921 cattgcccgt ttatcgcagc aatatgattt acgtgttgcc aacgtctttc atgccggaga
    16981 tggcaacatg cacccgttaa tccttttcga tgccaacgaa cctggtgaat ttgcccgcgc
    17041 ggaagagctg ggcgggaaga tcctcgaact ctgcgttgaa gttggcggca gcatcagtgg
    17101 ggaacatggc atcgggcgag aaaaaatcaa tcaaatgtgc gcccagttca acagcgatga
    17161 aatcacgacc ttccatgcgg tcaaggcggc gtttgacccc gatggtttgc tgaaccctgg
    17221 gaaaaacatt cccacgctac accgctgtgc tgaatttggt gccatgcatg tgcatcacgg
    17281 tcatttacct tttcctgaac tggagcgctt ttgatgctac gcgagtgtga ttacagtcag
    17341 gcgctgctgg agcaggtgaa tcaggcaatt agcgataaaa cgccgctggt gattcagggc
    17401 agcaatagca aagccttttt aggtcgccca gtcaccgggc aaacgctgga tgtgcgttgt
    17461 catcgcggca ttgttaatta cgacccgacc gagctggtga taaccgctcg tgccggaacg
    17521 ccgctggtgg cgattgaagc ggcgctggag agcgccgggc aaatgcttcc ctgcgagccg
    17581 ccgcattatg gtgaagaagc cacctggggc gggatggtcg cctgcgggct ggcggggccg
    17641 cgtcgcccgt ggagcggttc ggtccgcgat tttgtcctcg gcacgcgcat cattaccggc
    17701 actggaaaac atctgcgttt tggtggcgaa gtgatgaaaa acgttgccgg atacgatctc
    17761 tcacggttaa tggccggaag ctacggttgt cttggcgtgc tcactgaaat ctcaatgaaa
    17821 gtgttaccgc gaccgcgtgc ctctctgagc ctgcgtcggg aaatcagcct gcaagaagcc
    17881 atgagtgaaa tcgccgagtg gcaactccag ccattaccca ttagtggctt atgttacttc
    17941 gacaatgcgt tgtggatccg ccttgagggc ggcgaaggat cggtaaaagc agcgcgtgaa
    18001 ctgctgggtg gcgaagaggt tgtgggtcag ttctggcagc aattgcgtga acaacaactg
    18061 ccgttcttct cgttaccagg taccttatgg cgcatttcat tacccagcga tgcgccgatg
    18121 atggatttac ccggcgagca actgatcgac tggggcgggg cgttacgctg gctgaaatcg
    18181 acagccgatg acaatcaaat ccatcgcatc gcccgcaacg ctggcggtca tgcgacccgc
    18241 tttagtgccg gagatggtgg ctttgccccg ctatcggctc ctttattccg ctatcaccag
    18301 cagcttaaac agcagctcga cccttgcggc gtgtttaacc ccggtcgcat gtacgcggaa
    18361 ctttgaggag caggctatgc aaacccgatt aaccgaagag atgcggcaga acgcgcgctg
    18421 gaagccgaca gcatcctgtg cgcctgtgtt cactgcggat tttgtaccgc aacctgccca
    18481 acctatcagc ttctgggcga tgaactggac gggccgcgcg ggcgcatcta tctgattaaa
    18541 caggtgctgg aaggcaacga agtcacgctt aaaacacagg agcatctcga tcgctgcctc
    18601 acttgccgca attgtgaaac cacctgtcct tctggtgtgc gctatcacaa tttgctggat
    18661 atcgggcgtg atattgtcga gcagaaagtg aaacgcccac tgccggagcg aatgctgcgc
    18721 gaaggattgc gccaggtagt gccgcgtccg gcggtcttcc gtgcgctgac gcaggtaggg
    18781 ctggtgctgc gaccgttttt accggaacag gtcagagcaa aactgcctgc tgaaacggtg
    18841 aaagctaaac cgcgtccgcc gctgcgccat aagcgtcggg ttttaatgct ggaaggctgc
    18901 gcccagccta cgctttcgcc caacaccaac gcggcaactg cgcgagtgct ggatcgtctg
    18961 gggatcagcg tcatgtcagc taacgaagca ggctgttgtg gcgcggtgga ctatcatctt
    19021 aatgcgcagg agaaagggtt ggcacgggcg cgcaataata ttgatgcctg gtggcccgcg
    19081 attgaagcag gtgccgaggc aattttgcaa accgccagcg gttgcggcgc gttcgtcaaa
    19141 gagtatgggc agatgctgaa aaacgatgcg ttatatgccg ataaagcgcg tcaggtcagt
    19201 gaactggcgg tcgatttagt cgaacttctg cgcgaggaac cgctggaaaa actggcaatt
    19261 cgcggcgata aaaaactggc cttccactgc ccgtgtaccc tacaacatgc gcaaaagctg
    19321 aacggcgaag tggaaaaagt gttacttcgt cttggattta ccttaacgga cgttcccgac
    19381 agccatctgt gctgcggttc agcgggaaca tatgcgttaa cgcatcccga tctggcacgc
    19441 cagctgcggg ataacaaaat gaatgcgctg gaaagcggca aaccggaaat gatcgtcacc
    19501 gccaacattg gttgccagac gcatctggcg agcgccggtc gtacctctgt gcgtcactgg
    19561 attgaaattg tagaacaagc ccttgaaaag gaataaaaaa atgaaaacta aagtcattct
    19621 tagccagcaa atggcgagtg caattattgc cgcaggtcag gaagaggcgc agaaaaataa
    19681 ctggtctgtt tccattgctg ttgccgatga cggcggtcat ctgctggcgt taagtcgcat
    19741 ggacgattgc gcgccgattg cggcttacat ctcccaggag aaagcgcgta ccgccgcgct
    19801 ggggcgtcgt gaaactaagg gctatgaaga gatggtgaac aacggacgta ccgcgttcgt
    19861 gactgcgccg ttactgacgt cgctggaagg cggcgtaccg gttgttgttg atgggcaaat
    19921 tattggtgcc gtgggcgttt ctggtttaac cggagcacag gatgcgcagg tcgcgaaagt
    19981 ggcagcagcg gtgttggcga aataagcgaa aacgaggaga gaaacaatga gtcaaaccat
    20041 aacccagggc cgtttacgca ttgacgccaa ttttaaacgt tttgtggatg gagaagtttt
    20101 gccgggcgtg gaactggacg ctgctgcatt ctggcacaat gttgatgaga tcgttcacga
    20161 tctggcacca gaaaatcgtc agttgctggc agaacgcgat cgcattcagg cggcgcttga
    20221 tgagtggcat cgcagcaatc cggggccggt aaaagataaa gcggcctata aatctttctt
    20281 tcgtgaactg ggctacctgg tgccgcaacc ggagcgcgtg acggtggaaa ccacgggcat
    20341 tgacagcgaa atcaccagtc aggcggggcc acagctggtg gttccggcaa tgaacgcccg
    20401 ctacgcgctg aacgcggcga acgctcgctg gggctcactg tacgatgcgt tatacggcag
    20461 cgacatcatc ccgcaggaag gggcgatggt cagtggctac gatccgcagc gcggtgagca
    20521 ggttatcgcc tgggttcgac gtttcctcga tgaatcacta ccgctggaaa acggcagcta
    20581 tcaggatgtg gtggcgttta aggtggtcga taaacaatta cgcatccagt tgaaaaatgg
    20641 taaagaaacc acgttacgta ctccagcaca gtttgtcggt taccgtggcg atgccgctgc
    20701 gccgacctgc attttgctga aaaataacgg cctgcatatt gaactgcaaa ttgatgccaa
    20761 cgggcggatt ggcaaagacg atccggcgca catcaacgat gttatcgtcg aagctgctat
    20821 cagtaccatt ctcgactgcg aagattcggt cgcggcggtt gatgcggaag ataaaatcct
    20881 gctgtaccgc aatctgctgg gcctgatgca ggggactctg caagagaaaa tggagaaaaa
    20941 cggtcggcaa atcgtacgta aactgaatga cgatcgtcag tacaccgccg ccgatggctc
    21001 tgaaatttct ctgcacggac gctcgttgct gtttatccgc aacgtgggtc atttgatgac
    21061 cattcctgtg atttgggaca gcgaaggcaa tgaaatcccg gaaggtattc ttgatggcgt
    21121 catgactggc gcgattgccc tctatgattt aaaagtgcag aaaaactcgc gcactggcag
    21181 cgtctatatt gtgaaaccga aaatgcacgg tccgcaggaa gtggcgttcg ccaacaaact
    21241 gtttacccgc attgagacaa tgctcggtat ggcaccgaat accctgaaaa tgggcattat
    21301 ggatgaagaa cgccggacct cgctgaactt gcgtagctgt atcgctcagg cgcgcaaccg
    21361 cgtggcgttc atcaataccg gtttcctcga ccgtaccggc gatgaaatgc attcggtgat
    21421 ggaagctggc ccgatgctgc gtaaaaacca gatgaaatcg acgccgtgga tcaaagccta
    21481 cgaacgtaat aacgtgcttt ccggtctgtt ctgtgggctg cgcggtaaag cgcaaatcgg
    21541 taaaggcatg tgggcaatgc cggacctgat ggcagacatg tacagccaga agggcgacca
    21601 actgcgtgcc ggggcaaaca cagcctgggt tccgtcacca accgctgcta cgctccatgc
    21661 gctgcactac caccatacca acgtacagag cgtgcaagcc aacattgccc agaccgagtt
    21721 cagtgctgaa tttgaaccgc tgctggacga tctgctgact attccggtgg ctgaaaacgc
    21781 taactggtcg gcgcaagaga tccaacaaga gctggataac aacgtgcagg ggattctggg
    21841 gtacgtggtg cgttgggtgg agcaggggat tggttgttca aaagtgccgg atattcacaa
    21901 cgtggcgctg atggaagacc gcgcaacgct gcgtatctcc agccagcata tcgccaactg
    21961 gttacgtcac ggtattctga ccaaagaaca ggtgcaggcg tcgctggaga atatggcgaa
    22021 agtggttgat cagcaaaatg ctggcgatcc ggcttatcgt ccgatggcgg ggaatttcgc
    22081 taactcgtgt gcttttaaag ctgccagcga tttaatcttc ctcggcgtga aacagccaaa
    22141 cggttatacc gaaccgttat tacacgcctg gcgtttacgc gaaaaagaaa gtcattaata
    22201 gcgtcaaaaa agccccggca agttttattg tcggggctat gatagtaact gcggtagtac
    22261 atttatcccg gaaataaccg gagcgattat cagggtttaa caatattaaa tacccgtagc
    22321 gcattactag cgggcctaaa acaacaatat ggacatctta acgatactct gcgcctgcgt
    22381 gtgcggaaca gaaaccctta cccgatatac ttgttatgcc agaggtagca attgcgttag
    22441 cggcgtactg ttgctgtaaa gtataacgcc tatattttac agttcagtcc tcttttgctt
    22501 attacaacct taagtatatc aagcatataa agataataag agactgaaca atatggttac
    22561 ctggacccaa atgtatatgc cgatgggagg actggggcta tccgctctgg tcgccctgat
    22621 cccgataata ttcttcttcg ttgcactcgc ggtattacgt ctgaaaggac atgttgctgg
    22681 agcaataacc cttatattat ctatcctgat tgcgatattc gcctttaaaa tgccgattga
    22741 tatggcattt gctgctgcgg gctatggctt tatttatgga ttatggccaa ttgcgtggat
    22801 tattgtcgcg gcggtgttcc tgtataaatt aaccgttgcc agcgggcagt tcgatattat
    22861 ccgcagctcg gttatctcca tcaccgacga tcagcgtttg caggtgttac tgattggttt
    22921 ctcctttggt gcgttgctgg aaggagctgc tggctttggt gcgacggtgg cgtttggtgc
    22981 gttgggcgtg ccgattctgg tcgccggtca ggtaacggga atcgatccgt tccacattgg
    23041 cgcaatggcg ggacgtcagt taccgttcct gtcggttctt gtgccgttct ggctggtagc
    23101 aatgatggac ggctggaaag gggtgaaaga gacgtggcca gcggcgctgg ttgctggggg
    23161 aagcttcgct gtcactcagt tctttacctc taactatatt ggtccggaac tgccggatat
    23221 tacttcggcg ctggtgagta tcgtctcact cgctttattc cttaaagtct ggcggccgaa
    23281 aaataccgaa acggcaatca gcatggggca atccgcaggt gcgatggtgg taaataagcc
    23341 atcttctggc ggtcccgtgc cttcagaata tagtctgggg caaatcattc gtgcgtggtc
    23401 accgttttta atcttaacgg tgctggtcac catctggacc atgaagccgt ttaaagcgtt
    23461 atttgctccg ggcggcgcgt tttattcact ggtgattaat ttccagatcc ctcatttgca
    23521 tcaacaagtg ttgaaagcgg cacccattgt cgcccaacca acgccaatgg atgcggtgtt
    23581 taaattcgac cctctctcgg ctggcggcac cgctattttt attgcggcga ttatctctat
    23641 cttcatcctc ggcgtgggca tcaagaaagg gattggcgtc tttgccgaaa tgctaattag
    23701 tctgaagtgg ccgatattgt cgattggcat ggtgctggca ttcgccttcg tcaccaacta
    23761 ttccggcatg tccaccacgc tggcgctggt actggcaggt acaggcgtga tgttcccgtt
    23821 tttctcaccg tttctcggct ggctgggcgt attccttacc gggtcggaca cttcttcaaa
    23881 cgccctgttt ggttcactgc aatcgaccac ggcgcagcaa atcaacgtct ctgacactct
    23941 gctggtggca gcaaatacca gcggcggcgt aactggcaag atgatctccc cgcaatctat
    24001 cgccgtggcc tgcgccgcga cgggcatggt gggccgagaa tctgaactgt tccgctacac
    24061 cgtgaagcac agtctgattt ttgccagcgt tatcaccctg ctgcaggcgt atgtgtttac
    24121 cgggatgtta gtctcgtaaa tcaataaagc cggatgaagg taaatatcac ccggctttcc
    24181 atttgtctgt cgtttatctc gccagggaac cgtttttacg attggcttct ttatacgtag
    24241 tctccgtttt acgatgcgaa ttgtttaata tttatacaac ttggcttctg tgctggttat
    24301 aaagtaccca ctttcatgag atcaatttta gtatgttatt acattgcttc atctgttgct
    24361 ttcatgacga atggcgtttt ttctcttatt tatatttttc actagaacaa tgagtagatc
    24421 aaaattccca aaactcccaa aaacataaga ttattcgcaa agctgatttt gcagaataat
    24481 gagaactttt aagatttgtc gaggatcaat tttaagcaat ttatctccac ttgttacata
    24541 aaagatgccg ttaggttaat aagttaatct tgctataaca gtgttcgtta ctgcctatta
    24601 ataaagatat ttgggatagg gtaacgtgga tgaggattcg aaatcatccg tcttgcggaa
    24661 agtgaaatgt tggcagatgt taatattatg tatgtatttt aactccaaca acacaccaat
    24721 cattttgtta gaaataagaa aatttatggt taaataattt ggtgattatg aagcattaaa
    24781 tcgtaatgcc aaatattgtt cgctttgga
//
LOCUS       DAZOYF010000047        24647 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZOYF010000047 DAZOYF010000000
VERSION     DAZOYF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.047"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(65..157)
                     /locus_tag="ACHUOF_001791"
     tRNA            complement(65..157)
                     /locus_tag="ACHUOF_001791"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(121..123),aa:Ser,seq:gct)
     gene            complement(473..658)
                     /gene="csrA"
                     /locus_tag="ACHUOF_001792"
     CDS             complement(473..658)
                     /gene="csrA"
                     /locus_tag="ACHUOF_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR0610996.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(893..3523)
                     /gene="alaS"
                     /locus_tag="ACHUOF_001793"
     CDS             complement(893..3523)
                     /gene="alaS"
                     /locus_tag="ACHUOF_001793"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR0610997.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3651..4151)
                     /gene="recX"
                     /locus_tag="ACHUOF_001794"
     CDS             complement(3651..4151)
                     /gene="recX"
                     /locus_tag="ACHUOF_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR0610998.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4394..5455)
                     /gene="recA"
                     /locus_tag="ACHUOF_001795"
     CDS             complement(4394..5455)
                     /gene="recA"
                     /locus_tag="ACHUOF_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR0610999.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5535..6032)
                     /gene="pncC"
                     /locus_tag="ACHUOF_001796"
     CDS             complement(5535..6032)
                     /gene="pncC"
                     /locus_tag="ACHUOF_001796"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR0611000.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6177..7262)
                     /gene="mltB"
                     /locus_tag="ACHUOF_001797"
     CDS             complement(6177..7262)
                     /gene="mltB"
                     /locus_tag="ACHUOF_001797"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR0611001.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7518..8080
                     /gene="srlA"
                     /locus_tag="ACHUOF_001798"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7518..8080
                     /gene="srlA"
                     /locus_tag="ACHUOF_001798"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8077..9036
                     /gene="srlE"
                     /locus_tag="ACHUOF_001799"
                     /gene_synonym="gutE"
     CDS             8077..9036
                     /gene="srlE"
                     /locus_tag="ACHUOF_001799"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR0611002.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9047..9418
                     /gene="srlB"
                     /locus_tag="ACHUOF_001800"
                     /gene_synonym="gutB"
     CDS             9047..9418
                     /gene="srlB"
                     /locus_tag="ACHUOF_001800"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR0611003.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9422..10201
                     /gene="srlD"
                     /locus_tag="ACHUOF_001801"
     CDS             9422..10201
                     /gene="srlD"
                     /locus_tag="ACHUOF_001801"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFR0611004.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10306..10665
                     /gene="gutM"
                     /locus_tag="ACHUOF_001802"
     CDS             10306..10665
                     /gene="gutM"
                     /locus_tag="ACHUOF_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR0611005.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10732..11505
                     /gene="srlR"
                     /locus_tag="ACHUOF_001803"
                     /gene_synonym="gutR"
     CDS             10732..11505
                     /gene="srlR"
                     /locus_tag="ACHUOF_001803"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR0611006.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11498..12463
                     /gene="gutQ"
                     /locus_tag="ACHUOF_001804"
     CDS             11498..12463
                     /gene="gutQ"
                     /locus_tag="ACHUOF_001804"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR0611007.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12460..13974)
                     /gene="norR"
                     /locus_tag="ACHUOF_001805"
     CDS             complement(12460..13974)
                     /gene="norR"
                     /locus_tag="ACHUOF_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR0611008.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14161..15600
                     /gene="norV"
                     /locus_tag="ACHUOF_001806"
     CDS             14161..15600
                     /gene="norV"
                     /locus_tag="ACHUOF_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR0611009.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15597..16730
                     /gene="norW"
                     /locus_tag="ACHUOF_001807"
     CDS             15597..16730
                     /gene="norW"
                     /locus_tag="ACHUOF_001807"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR0611010.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16858..19110)
                     /gene="hypF"
                     /locus_tag="ACHUOF_001808"
     CDS             complement(16858..19110)
                     /gene="hypF"
                     /locus_tag="ACHUOF_001808"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR0611011.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19263..19790)
                     /gene="hydN"
                     /locus_tag="ACHUOF_001809"
     CDS             complement(19263..19790)
                     /gene="hydN"
                     /locus_tag="ACHUOF_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR0611012.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19939..20949)
                     /gene="ascG"
                     /locus_tag="ACHUOF_001810"
     CDS             complement(19939..20949)
                     /gene="ascG"
                     /locus_tag="ACHUOF_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR0611013.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(21011..21082)
                     /locus_tag="ACHUOF_001811"
                     /pseudo
     CDS             complement(21011..21082)
                     /locus_tag="ACHUOF_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21092..22801)
                     /gene="hycE"
                     /locus_tag="ACHUOF_001812"
     CDS             complement(21092..22801)
                     /gene="hycE"
                     /locus_tag="ACHUOF_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR0611014.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22819..23742)
                     /gene="hycD"
                     /locus_tag="ACHUOF_001813"
     CDS             complement(22819..23742)
                     /gene="hycD"
                     /locus_tag="ACHUOF_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR0611015.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23745..>24647)
                     /locus_tag="ACHUOF_001814"
     CDS             complement(23745..>24647)
                     /locus_tag="ACHUOF_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HFR0611016.1"
                     /translation="GIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGH
                     RDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVAR
                     LLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAIC
                     CVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRL
                     PSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGS
                     ALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT         5812 a         6937 c         6320 g         5578 t
ORIGIN      
        1 attcgaacct ccgaccgctc ggttcgtagc cgagtactct atccagctga gctacggatg
       61 caaatggcgg tgaggcgggg attcgaaccc cggatgcagc ttttgaccgc atactccctt
      121 agcaggggag cgccttcagc ctctcggcca cctcaccaca cgcctcttac gagtgcttcg
      181 aagaacttgt ttatcgctca tcatcgctgc gtggcgcaca tattactttc tgagacttat
      241 aagtcaaaca atttttccca cacttttatc gtttgcacac ttcacgctca attagtctgt
      301 aaaagcgtca aaaagagtgt tttatctaca gaaaaacgga ggtctggcag atagcagtaa
      361 tgcaaaaaaa tggagactta agttgaatga acgggagtaa agcgaaaaga ctatagagtg
      421 aaggagaaat tttgagggtg cgtctcaccg ataaagatga gacgcggaaa gattagtaac
      481 tggactgctg ggatttttca gcctggatac gctggtagat ctcttcacgg tgaacagaaa
      541 cttccttcgg ggcatttacg ccaatacgta cctggttgcc ctttacccct aaaactgtca
      601 cggtgacctc atccccaatc atgagggtct caccaactcg acgagtcaga atcagcattc
      661 tttgctcctt gaaagattaa aagagtcggg tctctctgta tcccggcatt atccatcata
      721 taacgccaaa aagtaagcaa tgacaaacac attacatgta agcagtcatg gcattacatt
      781 ctgttaaacc taagtttagc cgatatacac aacttcaacc tgactttatc gttgtcgata
      841 gcgttaatgc gaatgccgtg aagcgagtcc acggcattgc ctgacgctta tattattgca
      901 atttcgcgct gacccagcct ttcacactgg ctaacgctgc aggtaaggcc gcagcatccg
      961 taccaccggc ttgcgccatg tcaggacgtc caccaccctt gccgcccacc tgctgagcga
     1021 ccataccaat cagttcccct gctttcacac gatctgtgac gtccttagat acgcctgcaa
     1081 tcagagaaac cttaccttcg gctaccgttg ccagcacgat aattgtcgac cccagctgat
     1141 tttttaaatc gtcaaccatg gtacgcaaca ttttcggctc aacaccgcta agctcgctaa
     1201 ccaacagctt aacaccatta acatcaattg ccttactgga aagatttgcg ctctcctgtg
     1261 cggcagcttg ttctttaagc tgttgtagtt ctttttctaa ctgacgcgta cgttccagta
     1321 ctgagcgcac tttatccgcc agattattgc tatcgccttt cagcagatgc gcgacttcgc
     1381 ttaagcgatc gctgtctgca tgaacggtgg tgatagcgcc ttctccggtt accgcttcga
     1441 tacgacgaac gcctgcagca gtacccgatt cagagatgat gcggaacaga ccaatatcac
     1501 cagtgcggct ggcgtgagta ccgccacaca gctcggtgga gaaatcgccc atgctcagca
     1561 cgcgtacacg ctcatcatac ttctcgccga acagcgccat cgcacctttc gctttcgccg
     1621 cttcgagatt catgatgttg gtttcgatcg gcaaattgcg acgaatctgt gcgttcacca
     1681 ggtcttcgac cgcacgaatc tcttccggtt tcatcgcttc gttgtgtgag aagtcgaagc
     1741 gcagcacctt gtcgttaacc agtgaacctt tctgcgatac atgagtcccc agaacctggc
     1801 gcagcgcagc gtgcatcagg tgcgttgcgg agtgattcag acgaatacgg gcgcgacgag
     1861 cctcatcaat atcagcctgc acagcgtcgc ccactttcag agaacccgca gcaagtttac
     1921 cgatgtgacc aatcgcctgg ccgtatttct gcgtatcttc caccgcgaag gagaagttag
     1981 cgcctttcag ttcgccttta tcaccaacct gaccgccgga ttccgcatag aatggcgttt
     2041 gatccagcac gaccacagct tcctggccgg cattgatggc atcaaccgct ttaccatcaa
     2101 caaacagcgc ggtcactttg ccgttcagtt ccagatggtc atagccttta aattcagatg
     2161 cactatcaac acggatcatt gcgttgtaat cggcaccaaa gccgctggct tcgcgcgcgc
     2221 gacgacgctg ctcttccatt gctgcttcaa aaccagcttc gtcaactttg atgttgcgct
     2281 cacgacaaac atcagccgtc aggtcaaccg ggaagccata ggtgtcgtac agacggaaag
     2341 ccgtttcacc atccagcgta tcaccagaaa gttttgccag ctcttcatcc agcaacgcca
     2401 gaccgcgctc cagagtacgg gcaaactgct cttcttcagt cttcagcacc tgctcaacct
     2461 gcgcctgctg gcgtttcagg tcttcacccg cagagcccat aacgtcgatc agcggaccaa
     2521 ccagtttgta gaagaaggtt tctttcgcgc cgagcatatt gccgtgacgc actgcgcgac
     2581 gaatgatacg acgcagtaca taaccacggt tttcattgga cggcattacg ccatccgcga
     2641 tcaggaacgc acaagaacga atgtggtcag cgattacgcg cagcgattta ttgctcagat
     2701 cggtcgcgcc agtgactttc gctaccgcct ggatcaacgt gcggaacagg tcgatgtcat
     2761 agttagagtt aacgtgttgc agcaccgcag caatacgctc cagacccata ccggtatcta
     2821 cagacggctt cggcagcggt tccatcgtgc catctgcctg gcggttgaac tgcatgaaga
     2881 cgatgttcca gatctcaatg tagcggtcgc cgtcttcttc cggacttccc ggagggccac
     2941 cccaaatgtg gtcgccgtga tcgtagaaga tttcggtgca cgggccgcac ggaccagtgt
     3001 cacccatctg ccagaagttg tcagatgcgt atggcgcacc tttgttatcg ccgatgcgaa
     3061 taatacgttc gcgcgggatc ccgacttctt tttcccagat ttcgtaggct tcgtcgtcgc
     3121 tttcatagac ggtaacccac agacgctctt tcggcagggc aaaccatttt tcgctggtca
     3181 gcagttccca tgcaaactga atggcatcgt gtttgaaata gtcgccgaag ctgaagttgc
     3241 ccagcatttc gaagaaggta tggtgacgcg cggtgtaacc gacgttttcc aggtcgttgt
     3301 gtttaccacc cgcacgcacg cagcgttggg aagtggtagc gcgggaataa ttacgcttgt
     3361 cgagcccaag gaacacatcc ttgaactggt tcatcccggc gttggtaaac aacaaagttg
     3421 ggtcgttatg gggtaccagg gagctgctgg caactacctg atgtccctta ctatggaaaa
     3481 agtcgagaaa cgcctgacgg atctcagcgg tgctcttgct cataattatc ctgaaatcaa
     3541 gctaacgaaa tatcgccacc agctccagcg tgtcttaacc gccgggctgg taactgaaaa
     3601 gtgggaataa gataagtttt cttgactggg aagtaaaata ccgtatgcgt tcagtcggca
     3661 aaatttcgcc aaatatcctg gatatcttcc atcagatagc cacgatagag cagaaaacgc
     3721 tggatcttaa ctttttctga aaagacagtt ggcaaaggtt caccatattt tcgcgtcgcc
     3781 tgatcgcgcg ccagtgcgca ccagtcgatg tcacattcac gcatcgcttt ttctgtcgct
     3841 tcgcgggaaa tacctttctg attcagttcc tgacgaatac gtgcaggtcc ataccctttg
     3901 cggctacggc tggcgataaa gcgcgcaaca aatcggctgt catcgagata gccatgttca
     3961 tggcaccagg caataacgcg ctcgtaatct tctgccgtag catcaatctc ttctgggcca
     4021 tttttgccca taatcggtgc cgcgagttta cgtcgcagtt cttgctcact gtgatcgcgc
     4081 accgccagaa tgcgtaccgc acgatccaac aggcgagcat atgccgggcg acgggatgtt
     4141 gattctgtca tggcatatcc ttacaactta aaaaagcaaa agggccgcag atgcgcccca
     4201 ggttttgctg ataaacagat ttaatagtca atgagtatgc agaatgttcc cgcaggccca
     4261 attcgttaga ggatcgaggt gagagtaatt tttcacgcca gtgctggcct gttcccctca
     4321 ccctaaccct ctccccaaag gggcgagggg accgtccgtg cacacattat ctattatcat
     4381 caataacgaa cgattaaaaa tcttcgttag tttctgctac tccttcgctg tcatctacag
     4441 agaaatccgg cgttgagttc gggttgctca gcagcaactc acgtactttc ttctcgatct
     4501 ctttcgcggt ttccgggtta tctttcagcc aggcagttgc attcgcttta ccctgaccga
     4561 tcttctcacc tttgtagctg taccacgcgc ctgctttctc gatcagcttc tcttttacgc
     4621 ccaggtcaac cagttcgccg tagaagttga taccttcgcc gtagaggatc tgaaattcag
     4681 cctgtttaaa cggtgcagcg attttgttct tcaccacttt cacgcgggtt tcgctaccca
     4741 ccacgttttc gccctctttc accgcgccga tacgacggat gtcgagacga acagaggcgt
     4801 agaatttcag cgcgttacca ccggtagtgg tttccgggtt accgaacatc acaccaattt
     4861 tcatacggat ctggttgatg aagatcagca gcgtgttgga ctgcttcagg ttacccgcca
     4921 gcttacgcat cgcctggctc atcatacgtg ccgcaaggcc catgtgagag tcgccgattt
     4981 cgccttcgat ttccgctttc ggcgtcagtg ccgccacgga gtcaacgacg ataacgtcta
     5041 ctgcgccaga acgcgccagg gcgtcacaga tttccagtgc ctgctcgccg gtatccggct
     5101 gggagcacag caggttatcg atatcgacgc ccagtttacg tgcgtagatt gggtccagcg
     5161 cgtgttcagc atcgataaac gcacaggttt taccttcacg ctgcgctgcg gcgatcacct
     5221 gcagcgtcag cgtggtttta ccggaagatt ccggtccgta gatttcgacg atacggccca
     5281 tcggcagacc acctgcccca agcgcgatat ccagtgaaag cgaaccggta gagatggttt
     5341 ccacatccat ggaacggtct tcacccaggc gcatgatgga gcctttacca aattgtttct
     5401 caatctggcc cagtgctgcc gccaacgctt tctgtttgtt ttcgtcgata gccattttta
     5461 ctcctgtcat gccgggtaat accggatagt caatatgttc tgttgaaaca attatactgt
     5521 atgctcatac agtatcaagt gttttgtaga aattgttgcc acaaggtctg caatgcatac
     5581 gcagtagcct gacgacgcac cgcatcacgg tcgccgctga agcattcccg ccgggtaatg
     5641 ccttcaccgc gggcagtggc aaaagcaaac cagacggtgc cgacaggctt ctcttcactg
     5701 ccgccatccg gcccggcgat accactaata gacacggcat aatcagcacg agccgctttc
     5761 agtgcaccta tcgccatttc caccacgacg ggttcactca ccgcgccatg ctgcgccagc
     5821 gtctcttcgc gtacgccgat catctgcgct ttggcttcgt tactgtaggt gacaaatccg
     5881 cgttcaaacc aggcggagct accggcaata tcggtaatca ctttcgctac ccaaccaccg
     5941 gtacaagact cggcagttgt tacggttgcg ccacgggctt tcagcgcctg cccaacctgt
     6001 tcgcttaact gcatcagttc actgtcagtc atcacaacct ctgtcagtca aaaatttatg
     6061 ccggacaaga tagcactttc gtcaggaaga tggtgttggc gttgcaattt tacgaggagg
     6121 attcagaaaa aagctgatta gccagaggga agctcacgcc cccctcttgt aaatagctac
     6181 tgtactcgcg ccagcgccac ggcttgtcct aactgccaga ccgccattgc gtaatgggtg
     6241 ctgtggttgt aacgggtgat ggtgtagaag ttcggcagac cgtaccagta ctggtagccg
     6301 gtgccaacat ccagacgcag caggctggct tgttgatggt tgcccagcgg ctgctgtggc
     6361 gttaaacctg cggcggcaag ctgcgaaatg ctgtacttag ttttgaaacc atttggcaag
     6421 cctggagcct gaccgtttgc cattatcgcg acctgatcgc ctttcaccca gccgtgcgct
     6481 ttgaaatagt tcgccacgct accgatcgca tcaaccggat cccacaggtt gatatgcccg
     6541 tcgccgctga aatctaccgc atattgtttg taagacgacg gcataaactg tccgtagccc
     6601 atcgccccgg caaaggaacc tttcagattg agcggatcgt cctgctcgtc gcgcgccatc
     6661 agcaggaagg tttccagctc gccagagaaa tactccgcgc ggcgtgggta gttaaatgac
     6721 agcgttgcca gcgcatcgag gatgcgagtt ttccccatca cgcgccccca gcgggtttca
     6781 acgccgataa tcccgacgat aatttccggc ggtacgccat acacctgcca cgcgcgattc
     6841 aacgcatctt catactgatt ccagaaaacc acaccgttct gcacgttgtc cggcgtaata
     6901 aattttttgc gataacgcag ccatgcgccg ttcggacctg atggcggttt caccgatgtg
     6961 gttggtgcct ggttatccat cagccgcagt accgaatcca gacgcttcgc ctgggagaga
     7021 atttcctgca actgctgacg atcgaaaccg tgtttgttca ccattttgtc gatgaactgc
     7081 tgggcattcg ggttattagc gaaatcgccg cccatctgca tcacattgtg ctgcggctca
     7141 agcaggaagc cgccagacgg cgttccggtg gtcgtttcag tctcagtagg ttttggcttg
     7201 ctgctacagg cggcaagcaa cacaaaaagg ggaagcaatg ttacataacg acgcttgaac
     7261 atgaggggtc catttaacag attcaaccag gggcaagtat ggtaaagcat cacaccccgc
     7321 acaaggaagc ggtagtcact gcccgatacg gactttacat aactcaactc attcccctcg
     7381 ctatactttt attcaaactt tcaaattaaa atatttatct ttcattttac gatcaaaata
     7441 acacttttaa acctttcaac ctgattagat taggttgccg tttggtaata aaacaataaa
     7501 tcctgaagga gagaacaatg atagaaacca ttactcatgg tgcagagtgg tttatcgggc
     7561 tgttccaaaa gggcggagag gtgtttaccg ggatggtgac cggcattctt ccgctgttga
     7621 ttagcctgca ggttatcatg aacgcactga ttaattttat cggtcagcat cgtattgaac
     7681 gttttgctca acgttgcgcc ggtaaccctg tttcccgtta cctactgtta ccgtgcattg
     7741 gcacgtttgt cttttgcaat ccgatgacct taagcctggg tcgctttatg ccggaaaagt
     7801 acaaacccag ctactacgcg gcggcctctt atagctgcca ctcaatgaat ggcctcttcc
     7861 cccatatcaa ccctgcgaac tgtttgttta tcttggcatt gccagcggtc tgacaacgct
     7921 gaacctgcca cttggcccac tggcggtgag ttatctgctg gttggtctgg tcaccaattt
     7981 cttccgcggc tgggtgaccg atctgaccac cgccattttt gagaaaaaga tgggcattca
     8041 acttgaacaa aaagttcacc tggcaggagc aacatcatga cgcgtattcg gatcgaaaaa
     8101 ggaacgggtg gctggggcgg cccgcttgag ctggaagcca cgccgggcaa aaaaatcgtc
     8161 tatatcaccg ccggtacccg gcctgcgatt gttgacaaac tggcacaact tactggctgg
     8221 caggctattg acggatttaa agaaggtgaa cccgcggagg cggaaattgg tgtcgcggta
     8281 atcgactgtg gcggcacatt acgctgcggc atctatccga aacgacgtat tcccaccatt
     8341 aatatccact cgacgggcaa gtccggtccg ctggcgcagt acattgtgga agatatttat
     8401 gtctctggcg taaaagaaga aaacatcact gtagtgggtg atgcgacacc acaaccctct
     8461 tccgtgggcc gtgactatga caccagtaag aaaatcaccg aacaaagcga tggtttactg
     8521 gcgaaggtgg gaatgggcat ggggtccgcc gttgccgtgt tgtttcagtc tggtcgtgac
     8581 accatcgaca ctgtattaaa aaccattctg ccgtttatgg cattcgtctc ggcgctcatt
     8641 ggcatcatta tggcttctgg ccttggtgac tggattgccc acggtcttgc tccgctagcg
     8701 agccatccac tgggtctggt catgctggcg ctcatctgct ccttcccact gctttcacct
     8761 ttcctcggcc caggcgcagt tatcgcacag gttatcggcg tattgattgg cgtgcagatt
     8821 ggtctcggca atattccgcc gcatctggct ttaccggcac tgtttgccat caacgcgcag
     8881 gcggcttgcg acttcatccc ggtcggtttg tcgctggcgg aagcccgtca ggacacggtt
     8941 cgcgtcggtg tcccttctgt actggtgagc cgctttttaa ccggcgcgcc gactgtactg
     9001 atcgcctggt ttgtctccgg ttttatctat caatagaggc tgaaacatga ccgttattta
     9061 tcagaccacc atcacccgta tcggcgcgag tgccactgac gccctcagcg accagatgct
     9121 catcaccttt cgtgaaggcg cgccagcgga cctcgaagag tattgcttca ttcattgcca
     9181 cggcgagttg aaaggtgcac tccatcccgg tttgcaattt tcactcgggc agcatcgcta
     9241 tccggtgacc gctgttggca gcgtggcgga agacaacctt cgcgaactgg gtcatgtcac
     9301 cctgcgcttc gatggtttaa acgaagcgga atttccgggc actgtccatg tggcaggccc
     9361 tgtacccgac gatatcgcgc cgggatcggt tttgaagttt gaatctgtta aggagtaaaa
     9421 aatgaatcag gttgccgttg tcatcggtgg tgggcaaacc ttaggcgcgt tcctgtgcca
     9481 cggtctggct gccgaggggt atcgcgtcgc ggttgtcgat attcagagcg acaaagccgc
     9541 aaatgtggca caagaaatta acgccgaata tggtgaaggt atggcgtacg gttttggtgc
     9601 tgacgccact agcgagcaaa gcgttctggc gctctctcgt ggggtagatg aaatctttgg
     9661 tcgcgtggat ttgctggtct atagcgccgg aatagcaaaa gcagccttta tcagcgactt
     9721 ccagctcggc gattttgacc gttcgctaca agtgaatctg gtgggttatt tcctgtgtgc
     9781 ccgtgaattt tcgcgtttga tgatccgcga cgggattcag ggtcgcatta ttcagatcaa
     9841 ctcgaaatcc ggcaaagtgg gcagcaaaca caactctggc tacagcgcag cgaaatttgg
     9901 tggcgtcggg ctgactcaat cactggcgct ggatctggcg gagtacggca ttacggtgca
     9961 ttcactgatg ctcggtaacc tgctgaaatc gcggatgttc cagtcactgt tgccacaata
    10021 cgcgaccaag ctgggtatca aaccggatca agtcgagcag tattacatcg acaaagtacc
    10081 gctcaaacgc ggctgcgatt atcaagatgt gctgaatatg ctgctgttct acgccagtcc
    10141 taaggcgtcg tactgcaccg gacagtcgat caatgtcacc ggcggtcagg tgatgttctg
    10201 atcaacagcg gagatccgtt aaggatctcc gtgagactat agaatgcctg atgcgctacg
    10261 ctcatcaggc atacaggact tccgccacta cattaaggaa aagttatggt atccgcactc
    10321 atcaccgtcg ccgttatcgc ctggtgtgcg caactggcct taggcggctg gcaaatttct
    10381 cgttttaacc gtgccttcga cacactatgc cagcaagggc gggttggcgt ggaccgttcc
    10441 agcgggcgct ttaaaccgcg ggtcgtggtc gccatcgcgc tggacgatca gcagtgcatc
    10501 gtcgacacct tgtttatgaa aggactgact gtcttcgccc gaccgcaaaa aattcccgca
    10561 attaccggca tgcatgcggg tgatttacag cccgatgtga tctttcccca tgatccacta
    10621 tcacagaatg ctctatcatt ggcgcttaaa ctgaaacgtg gataatttcg ttgtgaatgt
    10681 tacttgcttg cgaagttatc attttgaaac ctaaatcagg taatcacgcc catgaaacct
    10741 cgtcagcgtc aggccgccat tctggagtat ctgcaaaagc agggtaaatg ctcggttgaa
    10801 gaattggcgc aatactttga caccacaggc acaaccattc gcaaagatct ggtcattctg
    10861 gaacatgccg gaaccgtcat tcgtacttat ggcggagtgg tgttgaataa agaggaatcc
    10921 gatccgccta tcgatcataa aacgctcatc aacacccaca agaaagagct gattgcagaa
    10981 gctgccgtta gttttatcca tgatggcgat tcgatcattc ttgatgctgg cagtaccgtt
    11041 ttgcagattg ttcccctgct ctcgcgcttt aataacatca cggtgatgac caacagcctg
    11101 catatcgtca atgcgctatc cgaactggat aacgaacaaa ctatcctgat gccaggcgga
    11161 acatttcgca aaaaatcggc ctcatttcac gggcaactgg cagagaatgc cttcgagcat
    11221 ttcaccttcg ataaattgtt tatgggcacc gacggcatcg atctcaatgc gggcgtaacc
    11281 acgttcaacg aggtttatac cgtcagtaag gcaatgtgca atgccgcgcg cgaagtgatt
    11341 ttgatggcgg actcatcaaa gtttggccgt aaaagcccca acgtagtttg cagtcttgaa
    11401 agcgtcgata agctaattac cgacgcaggt atcgatccgg cgtttcgtca ggcgctggaa
    11461 gagaaaggga tcgatgtgat cataaccgga gagagcaatg agtgaagcac tactgaacac
    11521 gggacgtcag acgttaatgc tggagttgca ggaagcaagc cgtttaccgg aacgtctggg
    11581 cgatgatttt gttcgcgccg ccaatatcat cctgcactgc gaaggcaaag tggtggtttc
    11641 gggaattggc aaatcgggcc acattggtaa gaaaatcgcc gcaacacttg ccagtaccgg
    11701 cacaccggct ttttttgtcc atccggcaga agcactgcac ggcgatctgg ggatgattga
    11761 aagccgcgat gtgatgctgt ttatctctta ctccggtggc gcgaaggaac tggatctgat
    11821 tattccgcgt ctggaagata aatctatcgc gctgctggcg atgaccggca aaccgacgtc
    11881 accgctgggc ctggcggcga aagcggtgct ggatatctcc gtagaacgcg aagcctgccc
    11941 gatgcacctt gcgccgacct ccagcaccgt caataccctg atgatgggtg acgcgctggc
    12001 gatggcggtc atgcaggcgc gcggttttaa tgaagaagat tttgcccgct cccacccagc
    12061 tggggcactg ggcgctcgct tgctgaataa agtgcatcat ctgatgcgtc gtgatgacgc
    12121 tatcccacag gtagcgttaa ccgccagcgt gatggatgcg atgctggaac tcagccgcac
    12181 cggtctgggg ctggtggcgg tatgtgacgc tcaacaacag gtacaaggcg tctttaccga
    12241 cggcgattta cgtcgctggc tggtaggcgg cggcgcactc accacgccag ttaatgaagc
    12301 gatgacgaca ggcggcacca cattacaggc acaaagtcgc gccatcgacg ccaaagaagt
    12361 tctgatgaaa cgcaaaatca ctgccgcacc agtggtagat gaaaacggca aactcaccgg
    12421 cgcaattaac ctgcaggatt tctatcaggc cgggattatt taatccttca atcccagacg
    12481 tttcgccagc cgatgcaggt tggcgacgtc agtttccagc attcgcgcac aggctgccca
    12541 gttgtgatga ttttgtgcca gtgcctggcg aatagtttca cgctggaacg cttctgtcgc
    12601 ttcacgcagg ttttgcttaa caacgggcac cgccgccgct tctggcggcg gcaacgtcac
    12661 ctcaggaaaa gcaaaatgtt gcgcctcaag aatcacttca tcgccgttgc gggtggctct
    12721 cgccagcact accgcccgat gaatagcatg ttccagttcg cgcacgtttc ccggaaaact
    12781 gtagtgttgc agtaaatttc gcgccccggc acttaatacc acgcgggaga gcccctgccg
    12841 caaacgacac tgctcgcaga aataccccgc cagcagaatg acatcatcgc cccgctcacg
    12901 cagcggcggc accgaaagtg gaaacacgct caggcgatga aacaaatcgg cgcggaatcg
    12961 ccctgccagc acctcttcgc gtaagtcgcg gttagtcgcc gccagcacgc gcacatcgac
    13021 ccgcaaacta cggtcatcgc caacgcgctg aatatcgcca tactgcaaca ccctcagcag
    13081 cttggcctgc aatgccaacg acaactcgcc gatctcatcg agaaacagag taccgttatc
    13141 cgccatttca aacttcccgc tgcggttact gatagcgcca gtaaacgctc ctttcacatg
    13201 cccgaacaac tcactttccg ccacactttc cggcagtgcg gcacagttga gatagaccag
    13261 cggattcacc gcccgtggcg aggcttcatg aatcgctttc gccaccagct ccttaccggt
    13321 tcccgtctca ccgctgatca gaacgttgag atcggacgcc gccacaatct caatctcttt
    13381 tttcagttgc gtcatgccag gggacaagcc aatcatctgc gtctgtttca ccgcttcaaa
    13441 cggcgtggca tcgcctggca gcatattctg gctttccagt tgttcaatca gcaacgcatt
    13501 gcttaacgct cccgccgcca gcgcggcaat cagccgtaac tcttcgtcgc tgaaaacatc
    13561 gaactgatcg ggctgcatcc cgtcgagcgt cagtgcgccg atcaggtttt gtccgacaaa
    13621 caatggcaga ccaacgcagg cgtgaacctt cagactctcc tgcccaggaa tcaaaccgtc
    13681 atagggatcg ggcaattcgc tgtctgcggg aaagcgcacc acatccccgg cgcgggcaat
    13741 cgcttccagc cgtggatgcc cttccagcgc aaagcgtcta ccgagtacat cctttgccag
    13801 accgtcgatg gcaagcggaa taaactgccg cgaatcgtaa cgtagcaacg cagacgcatc
    13861 gcactccagc acctgacgta gcgtggtgat caggcgctga aaacgatcct gatgaccaat
    13921 cccacgctgc aattcgatgg cgatattcgc cagcacatca acggaaaaac tcatctttgc
    13981 ctcactgtca atttgactat agatattgtc atatcgacca tttgattgat agtcattttg
    14041 actactcatt aatgagcata attttattta tagagtaaaa acaatcagat aagaaactgg
    14101 cacgcaatct gcaattagca agacatcttt ttagaacacg ctgaataaat tgaggttgct
    14161 atgtctattg tggtgaaaaa taacattcat tgggtgggtc aacgtgactg ggaagtgcgt
    14221 gattttcacg gcacggaata taaaacgctg cgcggcagca gctacaacag ctacctcatc
    14281 cgcgaagaaa aaaacgtgct gatcgacacc gtcgaccata aattcagccg cgaatttgtg
    14341 cagaacctgc gtaatgaaat cgatctggcg gatatcgatt acatcgtgat taaccatgca
    14401 gaagaggacc acgccggggc gctgaccgaa ctgatggcgc aaattcccga tacgccgatc
    14461 tactgtactg ccaacgctat cgactcgata aatggtcatc accatcatcc ggagtggaat
    14521 tttaatgtgg tgaaaactgg cgacacgctg gatatcggca acggcaaaca gctcattttt
    14581 gtcgaaacac caatgctgca ctggccggac agcatgatga cttacctgac aggcgacgcg
    14641 gtgctgttca gtaacgatgc tttcggtcag cactactgcg acgagcatct gttcaacgat
    14701 gaagtggatc agacggagct tttcgagcag tgccagcgtt actacgccaa tatcctgacg
    14761 ccgttcagcc gcctggtaac gccgaaaatt accgagatcc tgggctttaa cttaccagtc
    14821 gatatgatag cgacctccca cggcgtggtc tggcgcgata acccgacgca aattgtcgag
    14881 ctgtacctga agtgggccgc tgattatcag gaagacagaa tcaccatttt ctacgacacc
    14941 atgtcgaata acacccgcat gatggctgac gctatcgccc agggaattgc ggaaaccgac
    15001 ccacgcgtgg cggtgaaaat tttcaacgtc gcccgaagcg ataaaaacga aatcctgacc
    15061 aatgtcttcc gctcaaaagg cgtgctggtc ggcacttcga cgatgaataa cgtgatgatg
    15121 ccgaaaatcg ccgggctggt ggaggagatg accggattac gcttccgtaa caaacgcgcc
    15181 agtgctttcg gatctcacgg ctggagcggc ggtgcgatgg atcgcctttc cacgcgcctg
    15241 caggatgcgg gtttcgaaat gtcgctcagc ctgaaagcca aatggcgacc agaccaggac
    15301 gctctggagt tatgccgtga acacggtcgc gaaatcgccc gtcagtgggc gctcgcgccg
    15361 ctgccgcaga gcacggtgaa tacggtagtt aaagaagaaa cctctgccac gacgacggct
    15421 gacctcggcc cacgaatgca gtgcagcgtc tgccagtgga tttacgatcc ggcaaaaggc
    15481 gagccaatgc aggacgttgc gccaggaacg ccgtggagtg aagtcccgga taacttcctc
    15541 tgcccggaat gctccctcgg caaagacgtc tttgacgaac tagcatcgga ggcaaaatga
    15601 gcaacggcat tgtgatcatc ggttcgggct tcgccgcccg ccaactggtg aaaaatattc
    15661 gcaaacagga cgccactatt ccattaaccc tgattgccgc cgacagcata gatgagtaca
    15721 acaaacccga cctcagccat gttatcagtc aggggcaacg tgccgacgac cttacccgcc
    15781 agacggcggg tgaatttgcc gggcagttta atctgcgcct gtttccgcac acctgggtga
    15841 cggatatcga tgccgaagcc catgtggtga aaagccagaa taatcagtgg caatacgaca
    15901 agttagtgct ggcaaccggt gccagcgcct ttgtcccgcc tgtgcctggg cgtgagttaa
    15961 tgctgacgtt aaatagtcag caagagtatc gcgcctgtga aacgcaactg cgggatgccc
    16021 gacgcgtgtt gattgtcggc ggtggtttga ttggtagcga actggcgatg gatttttgtc
    16081 gtgcaggcaa agcggtcacg ctaatcgaca acgctgccag tattctggcg tcattaatgc
    16141 caccagaagt aagcagccgc ttgcagcatc ggttgacgga gatgggcgtt catctgctgt
    16201 tgaaatctca gttgcagggg ctggaaaaaa cggattctgg cattctggca acgctggacc
    16261 accagcgtag tatcgaagtg gatgcggtaa ttgccgccac cggactgcgc ccggaaacag
    16321 ccctggcacg acgcgccggg ctgacgatta atcgcggcgt ttgcgtcgat agttatctgc
    16381 aaaccagtaa tgccgatatt tacgcgctgg gcgattgcgc ggaaattaac ggtcaggtat
    16441 tgccgttcct gcagccgatt caacttagcg ggatggtgct ggcaaaaaat cttctcggca
    16501 ataacacgcc gctgaaacta ccggcgatgc tggtgaaaat caaaacgccg gaattaccgc
    16561 tgcatctggc aggcgaaacc cagcgtcagg atttacgctg gcagattaat accgaacgcc
    16621 agggaatggt ggcgcgcggc gttgacgatg ctgaccagct tcgcgccttt gtggtcagtg
    16681 aggatcggat gaaagaggca tttgtattgt tgaaaacgtt gccggtgtag gtgggctact
    16741 gtgcctaaaa tgtcggatgc gacgctggcg cgtcttatcc gacctacggg gacgcatgtg
    16801 taggccggat aaggcattta cgccgcatcc ggcaagggtg tccaaatgca acacgcttta
    16861 tccgttctgg acttcacccg ctaaccaacg cgccgcagca ataaccccct gccccagaga
    16921 caaaccgcca tcacccgccg gtaaactctg tggaaagagc aatgtgaaat cagcgagata
    16981 atgcgccaga cgtgcacgca gcaaacggtt atgaataacc ccgccgctaa ataccagcgt
    17041 agtgatacca cgcatcgtgg cctgctcacg catcaacgcg gcaaaaccct gcgccagcgc
    17101 atcatgaaac gcccacgcgc gttgattaac cagtgcctgc cagttcagcc actgctgcca
    17161 gaaagttgcg agatccagtt gattgtccac ccgcggcatc gtcaccggat gcgtcactcc
    17221 gtcgcatgag gctgcgagcg cctccagagc acaagccgct tcaccttcat aacttaacgt
    17281 ggctggcgca cagcccagtg ccgccgccac tgcatcgaac aaccgcccac acgatgatgc
    17341 cagcggcgcg ttaattccac gctcaatggc ccgcgccagt acgctccagt tttgctgttg
    17401 cacacttgcc gtttcagggt aattctgcca ctccggcaca aagcgcaggc actgcgccag
    17461 caggtttcgc cacggctgct tcgccgccaa atcgccaccc ggaagcgcca ctgcaggcaa
    17521 gccgcccagg tgctcacatt cgcgatagtt cacccgcagg cactcgccgc cccacaaagc
    17581 gccattctcc cccataccga taccgtcgag cgtcaaagca atgacatcac cgccatccag
    17641 cggccactga tgctctgcca gacacgccgc tgcatgggca tgatgatgca gtaccgtttg
    17701 cgtcggcaga ttcatttcgc tcgcccactg gctggagaca tagcccggat gcacatcatg
    17761 cacaatgtat tgcggagtga aatcgtagat gttttgcatc aggcgtaacg cttcgcgcca
    17821 ctgcatctgg atgccatcgt cacttaaatc gcccagatgc tgactcaaca ccgcttgttc
    17881 accgcgcacc aggcagaagg tatttttcag atccgcgcca agacacagca caggcggaac
    17941 atttttaaag cccggaggca aaaccagcgc atccggcaca tacccccgcg aacggcgcag
    18001 catttcgccg ctttcgcgca ccactgaatc atccatccgc tgcacgatgt cgcggttatg
    18061 tagcaagaat ccgtcggcaa tgccctgcaa atccgccagc gcctgttcgt tgctgatagc
    18121 tggtggttta ccgctcaggt tgccggaggt catcaccagc gggcattgca gttcctgtaa
    18181 cagcaaatgc tggagcgggt tcgcaggcaa catcaccccg acttcgttaa ggccaggggc
    18241 gatatcatca caaagctcag gtacgtattt tttatccacc agcacaatcg gcgcagcggg
    18301 cgtggtaagc aactggcgcg cagcgtctgg taaactttct gccactggca acatgactgc
    18361 cagcggtttc gccgggcgat gtttgcgctc ccgcagtgtc gccaccgcgt tactgttacg
    18421 tgcatcgcag gcaagatgaa atccgccaat ccctttgatg gcgacaatgt tgcccatttt
    18481 taactgtgcg atagctgcct gtaatgccgc ctcttgttct gcatgttcac catgacttac
    18541 ccattcaaga tgcgggccac actccgggca ggccaccggc tgggcgtgga agcgacgatc
    18601 gagcgggtcg cggtactctt tgtcacaggc cggacatagc ggaaacgccg ccatcacggt
    18661 aaacgggcgg tcgtaaggca tcgcgcgaat aatggtgaaa cgcgggccgc agtgggtaca
    18721 gttgataaac ggataacgat aacgccgctc gcctggggta ttcatttcgg caaggcaagc
    18781 agggcaagtg gcggcatccg ggacaatttg cgtattcatg gtgccgccgg tgctctggcg
    18841 gatggtgaac tcggtgggca gttgtgacca gataaacggc tcacgctcga cgctatcaat
    18901 acgcgccagt ggcggacagt gctgatgcaa ttgaacaaga aacgtttccg ggtcttccag
    18961 cagccggact tctacgccat cgccgtcatt acatacatcg ccgtgaagat ttaattgctg
    19021 tgccagctgc cagacaaacg gacgaaaacc gacgccctgc actttgccac gaatacgcag
    19081 ttggacaccg caagatgtgt tttttgccat tgagttattc ccgccatcat gaattgcgta
    19141 acccgccctg ccggacacga cagcgtcgta tccggcagtc tcaggtcggc gataccgccc
    19201 gctccgtatt ctacgaatat ttccgggaat tcctttgatg ccagaacagt tctgtaagat
    19261 ttttagaaca tcagcgccgt acggcggcgt ttttctgcgc tcagttgttc aagtttatta
    19321 cgatcgacac aaatcagcgc atgggtcggg caagccgcca tacacgccgg gccgtcttca
    19381 cgatggttgc acaggtcgca tttattggct tcggctttgt cagcccgtac attcagaccc
    19441 gcgccgctgt tgcgaatcac aggacgtacc accacttcca tcgcgccata cgggcaagcc
    19501 accacgcagg ttttgcaacc aatgcaacgt tcctgcatca caggaacaaa ccctttatca
    19561 cggctgatag caccattcgg gcagacgtta gcgcacggtg catcttcaca ctgacggcaa
    19621 actgtcgccg tggaaatgtt cacaccttta atgacatgga tacgcggtaa aaatgtttcc
    19681 ggggtcagcg atgcacagtc ctgattttcc tgatgagaaa ccacgcacgc cacttcacag
    19741 gtacggcaac caatacattt actcgcgtca gcaatgatga aacggttcat caaattctcc
    19801 agcaatgaca gttaatgcgc cgatacactc acaaatcatg ccagttttta atttgctgtt
    19861 atttaaggaa attaatttct gtaatgcagg aaaaaagatg tcatcgacac tagtgacgat
    19921 gacatgtgat gacaatgttt atcgcgaaag ggcaatgagt gagtcgcggc ggatcagttt
    19981 tccgctaaag gttttcggcg gtgagaaatc cccgccatcg agcataaaaa tcagccgtcc
    20041 aataatttcc tgaatcatct cagtcaccgg aatttttacg ctggagagcg ccggaacgat
    20101 atagggggca attgcgatat catcgaatcc gataactgac acctgctctg gcaccgctac
    20161 gccgcgctcg tgcaacgctt tgatcgcacc tatcgccata tcgtcgttac tggcaactaa
    20221 cgcgctaaat ttagcccctc gttcgagcaa cgtttctacc ccttcggccc cgctggcggg
    20281 cgtccattta ccgttagcga taagtttttc attgagcgca ataccatgct gcgacagcgc
    20341 gtctttatac ccggcaagac gttcaatgct ggtgggggaa tccatcgagc cggtaaggaa
    20401 agcaatctcc tgatgcccgg cgtttatcaa ctctgccacg gcgttaaagc tggtctgttt
    20461 atgatcgcac cagacgctat ggctgctatt attgcgcagg cggcgattaa gcaccattat
    20521 cggctgactg tgcgcgtcaa tgatgtcatc aatctcatcc acgcttaaaa aacgcgggta
    20581 aatcataatg gcgtcgcagc gcaaatccag cagatactga atcgcctggc gctcttcttc
    20641 ggcgctgtgt ttaccatctg ccaatagcaa ctgtcgccct ttctcttccg ccattcgcgc
    20701 ggcatgaaac agtaattcac taaaataaat gccgtgataa agcgtgttgg tcactaccag
    20761 ccccagcgtc tgagtactct tcgccgacag attgcgggcc agcaaatttg ggcggtaact
    20821 gctctcttct accgcctgaa acacgcgatc tttagtctcc tggctgacgt agccattacc
    20881 tgaaagcacg cgggaaacgg tcgcttttga aaccccggcg cgcttcgcca cttccagcat
    20941 cgtcgtcatc attttcatcc ctttacacgc aatcaacgca gtgtactgca ccgtttgccg
    21001 attgtccttg cgcaatcggc tccagcggat aagacgaggt cgccgtgccg gttttgatga
    21061 cttttttgat aaaggtaaac atggcgattc cttatttcag cggcgagttt ttacgctcaa
    21121 tgctgtaacg ctcgagttct ttgtacggca ccactttgct cttcttctta cgcacatcga
    21181 ctacggtcat gcggtcggta caggagtagc aagggtccag gctaccgata atcagcggcg
    21241 cataggaaac ggtattgcca cgcagcatgt agcgcagggt cggccagttc gcgtaggtcg
    21301 cggcacggca gcgccagcgg tacagcttct ggttgtcgcc ggtcatgctc cagtggatat
    21361 cgtcgccgcg cggcgcttcg gcaaagccca gcgcgaaacg gtgcggaatg taggtaaagc
    21421 cttccaccat cagcggaccg cccggcaggt tatccagacc gtagtcgatc atattcagcg
    21481 cggtatagac ctcgttgatt cgcactttca gacgggaaat aacgtcgcag ccctgctcgc
    21541 tgtggacttc cattggcagc aggccataac cgacaaacgg gtgatcggcg cgagtatcgc
    21601 gggcgagacc gctggcgcga accatcgggc caacgttact gaaatcgcgg gcgatttccg
    21661 ggtccagacg accaatgccg acggtgcgtt gttccatgtt cggcgtactc agcagcacat
    21721 ccaccagctc ctgcacttca cgacgcattt gttgcgccag ctggcgggtc tggatcatgt
    21781 catctttcag cagatcgcga cgaatcccgc cgatcaggtt caggccgtag gttttacgcg
    21841 caccggtaag gatctctgcc attttcatgg aggtttcacg cacgcggaag aactgcataa
    21901 agccggagtc gaagccggta aagtgacagg cgaggccgag gttgagcaga tgcgggtgca
    21961 ggcgttccac ctccagcaga atggcgcgga tcatctgcgc gcgttctggc acctgaatac
    22021 ccatcgcgtt ttccaccgac gtggtgtagg cggtgctgtg ggcaaagccg cagatcccgc
    22081 acacacggtc agagaggaag gtcacttcgt tataacccat gcgggtttcc gccagttttt
    22141 ccataccgcg atggacgtag aacagacggt agtcggcgtc gataatgttt tcgccatcga
    22201 cgaacagacg gaagtgaccc ggttcgtcag aagtgacgtg cagcggacca atcggcacga
    22261 cgttgttttt cttgtcgccc agttcgttga tgaactcgta ggtttcagca tcggtagtcg
    22321 gtgccggacg ctgacgataa tccatgctgt ctttacgcag cggataaagt tcatccggcc
    22381 agtcatccgg cagcaccaga cgacgttcat ccggcagacc aaccggaatc aaaccgtaca
    22441 tatcgcgcac ttcgcgctcg ccccacaccg ccgccggaac gcgcggcgtc acggacggat
    22501 actccggttt gttggcgtca acttcgacgc gaaccgttac ccaacacttg gtgcccttct
    22561 ccatcgacag cacgtaataa acggcgtaat gaccattcag tttgcgttcg tcgttaccaa
    22621 acagcaccga cagccagcca ccctgcttgt agtagagaaa ctccaccact tccggcagat
    22681 agttcacctt tacggtgaca gtcagctgat ctttggtctg ccaggcgtgg tccagcacga
    22741 cgcctagaaa tgcctcattc agcgcggcga gataatgttg acctaatttt tcttcagaca
    22801 tgctcaaact ctctttaatc acgccgccag caaggagacg aacgctaaaa atgcaaagcc
    22861 aaacccagcc caggtaatgc gcggagtaat atcaagacgc agacgggcca tgctgttttc
    22921 gaacagcgcg ataaccagga cgccgaccac cagtttgacg atggcaatca ccagcgccag
    22981 cagcagtcca ccgacggtga aggtttccat ttgtccccac ggaataaaca ccccgacgaa
    23041 catctgcaac accaccagct gtttcaggct gataccccat ttcatgacgc caaagccgct
    23101 accgctgtat tcggagagcg gaccttcctg caactcctgc tcggcttccg ccaggtcgaa
    23161 cggcagtttg cccatttcga taaaagtggc gaacgcacag gcgcagagcg ccagcaccag
    23221 cgggatgctt tggctcagcg gccagtgata aacggtgtcg gtaatgttgc tgatgttggt
    23281 ggaaccggca acctgtgcgg caacccacag accaagcagc aacatcggtt cgaccagcac
    23341 gccaagcatc gcttcacggc tcgcaccgat agcggtaaac gggctaccgg tatccagacc
    23401 ggaaatggca aagaagaaac gcgcgatggc gaagagatac agtaaggtga tcaaatcgcc
    23461 cagttgcggc agcggagaac cgacggtcac caccggcagc gcagtagcga tagtcagcat
    23521 gacgcccacc atcacatacg gcgtcaggcg gaacatccag ccagaggcat ccgggccaac
    23581 gctctggcgg cccagcagtt tgataatgtc gcgatactcc tgcaacacgc ccggcccgcg
    23641 acggttatgc agacgggcgc gcgccacgcg ggtaataccg gagagcagcg gcgcaacggc
    23701 aaataacacc agcgcctgaa ttaacggata taaaacactc attctcaggc tcctcgtgaa
    23761 acaataatca ccaccagtac cgccagttca accagcgcca tccggcggaa cagcaacgca
    23821 ctaccttcgc actgccagcc cggcactagc gacaccggat tcagccattt gcgcagtttc
    23881 agcactggcg caaacgcctg tttaaccggc atggcaaaac cgtgagcggt aatcaccatt
    23941 gatttttcat ggtcgtagcc gcacacccag gccgcaccgc gggaacgtga cggcaggcgg
    24001 tcgcctttgc aaatcgccat aatgatgaat ggcagcagcg ggcaggcaat cagcagcaat
    24061 gtgatcattg gttgagaaac ggtggtgtta gcaggctcca gaggcagagg tacagcagca
    24121 gagagcatcg gcagcagcca cggcgcagca acaccgccaa ttacgcagca aatcgccagt
    24181 gccactacgc ttacgctcat caggagcggc gcacaggtgg cgttttcggc ttctttggta
    24241 cgcggcgcgc cgaggaacgt gacgccatag actttcgcca tacacatcac cgccagcgca
    24301 ccggtaattg ccagacccac agcgagcagc ggccccagca gacgggcaac aaacgcgccg
    24361 ctattgctca gtttgaagaa ggattgatag ataacccatt ccccggcaaa cccgttcagt
    24421 ggcggcagcg cagccattgc catcaacccg actaacatgg cgatggagat aaccggcatt
    24481 ttcttgccaa taccaccgag tttttcgata tcgcgatgac cggtacggaa ccagacgctc
    24541 cccgccccga ggaacagtac gcttttgaac aggctatggt taagcagatg gtacagacca
    24601 ccgaccaggc caagagcaat cagcgccggt tgttcgagcg cgatacc
//
LOCUS       DAZOYF010000048        24573 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZOYF010000048 DAZOYF010000000
VERSION     DAZOYF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.048"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(13..519)
                     /gene="mug"
                     /locus_tag="ACHUOF_001815"
                     /pseudo
     CDS             complement(13..519)
                     /gene="mug"
                     /locus_tag="ACHUOF_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
     gene            644..719
                     /locus_tag="ACHUOF_001816"
     tRNA            644..719
                     /locus_tag="ACHUOF_001816"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:677..679,aa:Ile,seq:cat)
     gene            complement(773..1518)
                     /gene="nfeF"
                     /locus_tag="ACHUOF_001817"
                     /gene_synonym="yqjH"
                     /pseudo
     CDS             complement(773..1518)
                     /gene="nfeF"
                     /locus_tag="ACHUOF_001817"
                     /gene_synonym="yqjH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
     gene            1795..2418
                     /gene="nfeR"
                     /locus_tag="ACHUOF_001818"
                     /gene_synonym="yqjI"
     CDS             1795..2418
                     /gene="nfeR"
                     /locus_tag="ACHUOF_001818"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR0611017.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(2572..4092)
                     /gene="aer"
                     /locus_tag="ACHUOF_001819"
                     /pseudo
     CDS             complement(2572..4092)
                     /gene="aer"
                     /locus_tag="ACHUOF_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            4510..5889
                     /gene="ygjG"
                     /locus_tag="ACHUOF_001820"
     CDS             4510..5889
                     /gene="ygjG"
                     /locus_tag="ACHUOF_001820"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR0611018.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5931..6262)
                     /locus_tag="ACHUOF_001821"
                     /pseudo
     CDS             complement(5931..6262)
                     /locus_tag="ACHUOF_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            6480..7463
                     /gene="ebgR"
                     /locus_tag="ACHUOF_001822"
     CDS             6480..7463
                     /gene="ebgR"
                     /locus_tag="ACHUOF_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR0611019.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            7646..10732
                     /gene="ebgA"
                     /locus_tag="ACHUOF_001823"
     CDS             7646..10732
                     /gene="ebgA"
                     /locus_tag="ACHUOF_001823"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFR0611020.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            10729..11178
                     /locus_tag="ACHUOF_001824"
     CDS             10729..11178
                     /locus_tag="ACHUOF_001824"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR0611021.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            11241..12674
                     /locus_tag="ACHUOF_001825"
     CDS             11241..12674
                     /locus_tag="ACHUOF_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0611022.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            12808..13879
                     /gene="ygjJ"
                     /locus_tag="ACHUOF_001826"
                     /pseudo
     CDS             12808..13879
                     /gene="ygjJ"
                     /locus_tag="ACHUOF_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            13896..16247
                     /gene="ygjK"
                     /locus_tag="ACHUOF_001827"
     CDS             13896..16247
                     /gene="ygjK"
                     /locus_tag="ACHUOF_001827"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR0611023.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATQEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            16473..18491
                     /gene="fadH"
                     /locus_tag="ACHUOF_001828"
     CDS             16473..18491
                     /gene="fadH"
                     /locus_tag="ACHUOF_001828"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR0611024.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18536..18952)
                     /gene="higA"
                     /locus_tag="ACHUOF_001829"
     CDS             complement(18536..18952)
                     /gene="higA"
                     /locus_tag="ACHUOF_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR0611025.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18949..19263)
                     /gene="higB"
                     /locus_tag="ACHUOF_001830"
     CDS             complement(18949..19263)
                     /gene="higB"
                     /locus_tag="ACHUOF_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR0611026.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(19548..20684)
                     /gene="rlmG"
                     /locus_tag="ACHUOF_001831"
     CDS             complement(19548..20684)
                     /gene="rlmG"
                     /locus_tag="ACHUOF_001831"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR0611027.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            20769..21272
                     /locus_tag="ACHUOF_001832"
     CDS             20769..21272
                     /locus_tag="ACHUOF_001832"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR0611028.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            21349..22041
                     /locus_tag="ACHUOF_001833"
     CDS             21349..22041
                     /locus_tag="ACHUOF_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR0611029.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            22120..23118
                     /locus_tag="ACHUOF_001834"
     CDS             22120..23118
                     /locus_tag="ACHUOF_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0611030.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            23389..24354
                     /gene="alx"
                     /locus_tag="ACHUOF_001835"
     CDS             23389..24354
                     /gene="alx"
                     /locus_tag="ACHUOF_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR0611031.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         5876 a         6463 c         6406 g         5828 t
ORIGIN      
        1 cggtgacgtt ttttatcgcc cacgcactac cagcgcctgg tccagctcgc gatacgcttc
       61 aaccagtttc tccagtgaaa cgcgacttaa accgctggga tttggcagca cccaaatctg
      121 cgtcgaacca atggtgagcg tttgtttccc ccactgtgca ccgcgctagc tgaatccctg
      181 ttcatatgct tgtttgccca gaatagccaa cgcctgcggc tgataatctt caattttttc
      241 aatcagctta cgcccacctg cgtgcagctc ctgctttgaa acttcattgg cttgcaccgt
      301 tggacggtct accagtttgg tgatgccaca acgataatcc agcagatgct gtgcctcctg
      361 cggcttcaac tgacggtcgg taaacccggc ctgatatatc accttccaga agcgatttgc
      421 cggatgagca aagggaaaac cagtcccggc ggatgaaagc ccagggttga taccgcaaaa
      481 cacgacccgt aaccctggag ccaaaatatc ctcaaccata tttactcctt tctaacatca
      541 ccaggaaaag tataacggat tgaaaataca ttgtttataa aaacagcagg cgcgcggtaa
      601 tggctggatt gcggcacgga gttactttat aatccgctac catggcccct tagctcagtg
      661 gttagagcag gcgactcata atcgcttggt cgctggttca agtccagcag gggccaccag
      721 atatagcaaa ggctgacgag aaatcgtcag cctttttctt tttatatatc agttactttg
      781 cgtgccagta agccgctgca cgtacccgct gtgggtcata ctgttccgct tcaaagcggc
      841 ggcttaaatt cttaacgact ttaccttcgc cggttatcca gatgaagtaa tcatcggcag
      901 ggatttgcat ctgcgccaga cgcgcatcta ccgcctgctc atcatgtgcc agccattcga
      961 tattaaaacc atcaaggtgc gcgagataat cctgacaggc gttatcccgc acgctaacca
     1021 gcgcactaac ttgcggttta acggcaagtt tgctcaacgt ttccaggcgg cggcgcagtg
     1081 cgggcattcc ggattcatcg cagacataca gctgatacgc gtaatcttcc ggcaccacca
     1141 gcgaaccgcg cggacctgcc accgtaagtt tatcgcccag ccgctggcga ccccaccgtc
     1201 gtgaataaag aaatcgatcg ccagttcatg gcgtagttcg tcatacagtg gcgtatagtc
     1261 acgcgacggt gggcgtggtc cttccggcca gacgatgctc tcttccgtta ccgttggcgg
     1321 cacaaagtga gcgtcaggtt gaggaaagaa gagtttgctg tgatcgtcaa agccgcgcga
     1381 tgtaaaaccg tccaacgcct cgccgccaag gacaatgcgc tgaaaaccgg cgctgatgcg
     1441 ctcaacgcgt agcacagtca gttcacggaa gcgcagatca ttgcgaacgc gctgcgggta
     1501 gcggggggtg ttattcattg ttatcgcctt cgtgatggta atcagatata tctaaataaa
     1561 attcgcaaat gataatgatt gttaatcatg ataaatgcaa gcgatttgta gaactgatat
     1621 gtctgaagta tgtgaaggca ttcagtgcca tagcaatgct gccggatgcg gcgtgaacgt
     1681 cttatccggc ctagatgtga ggtgcttcca cgtttaaaaa tcatttttgt acttgcagta
     1741 atgctcacat cagatataaa ttagatatat ctaatcaagc aaaaggaggc tgatatgagc
     1801 catcatcacg aagggtgttg taaacatgaa ggccagccac gccatgaggg ctgctgcaaa
     1861 ggtgagaagt cagaacacga gcactgcgga cacggtcacc agcatgaaca cggtcaatgt
     1921 tgcggtggtc gccacggtcg cggcggcggt cgtcggcaac gtttctttgg tcacggtgaa
     1981 ttgcgtctgg tgattctgga tattctctcg cgcgatgaca gccacggtta cgaattgatt
     2041 aaagcgattg agaatctaac ccaggggaat tacaccccaa gcccgggcgt catctacccg
     2101 acgctggatt ttctgcagga gcagtcgctg attaccatcc gcgaagagga aggtggtaag
     2161 aagcagattg cgctgaccga acaaggcgcg cagtggctgg aagaaaaccg cgaacaagtg
     2221 gagatgattg aagaacgcat caaagcgcgt tgcgttggcg cggcgctgcg ccagaacccg
     2281 caaatgaagc gggcgctgga taattttaaa gcggtgctgg atttacgcgt caaccagagc
     2341 gatatcagtg atgcacaaat aaaaaagatc attgcggtga tcgaccgcgc cgcttttgat
     2401 attacgcaac tggattaatc gccgcatccg ccagtggcgc ggtgcaattg ccggatgcga
     2461 cgcttgacgc gccttatccg gcctacaccc gctacacacc ccgcaggcct gataagatgc
     2521 gccagcatcg catcaggcat tgtgctccaa ccgccggatc cggcataccg attaatgcag
     2581 caccgtcacc gcgtcttcca gtcggctggc gcggtgtttc accatcgccg acacctgcgc
     2641 actctcttcc accagctcgg catttttctg ggtgatcagg ttaagctcat ccactgcacg
     2701 ggtcaggctg gaaagcccat cggcctgttc cagcgttgaa tggctaatct gggcgatcaa
     2761 ctgggtgacg tttttcacct gtgccacaat atcttccatc gtccgtccgg cggcgtgtac
     2821 ctgctgcgaa ccggattgca ccttatcagc actggcatca atcagcttgc gaatatcgtt
     2881 ggcagcatta gcgctgcggc tggctaaatg acgcacttcc cctgccacca ctgcaaaacc
     2941 tttgccctgt tcgccggcac gcgccgcttc caccgccgca ttcagggcca gaatattggt
     3001 ctgaaacgca atatcgttaa tcagcgaagt aatggtgcca atgcgctagg tactgtcggc
     3061 gatatcgtcc attgtcttga tcaccgtggt catcgcctcc ccaccctgca ctgccgcatt
     3121 actggcggtg atagaaagtt tatcggcagc cgacgccgtg gcagagttct gtttcaccga
     3181 cgccgccatt tggttcatgg tcgccaccgt ttgctgaacg ttatcaactg tctgctgggt
     3241 atgttcgttc agttcatcgg tgcctttcgc cagcgtctca ctgccatttc tgacactgga
     3301 cacctggctt gagacatcgt taattagcca acggcacatc aggccaagct gccctaccgc
     3361 gcgtaatgtc agccccagct catcgctgcg attcaggtgc tcaacactat tacgctctcc
     3421 ggtcgccacc ttcagtgcct gacgggcgac attttctatc gggcgcacaa tctgccattc
     3481 aaaacaagcg cttgccaaca ataccactaa cacacagagg aaatacgtca ccaccggggc
     3541 agcaacaaac caaagcatgg ccgccagcaa gataaacatc agggtcatca ctccacgcgt
     3601 ccgccagcga agcggtaatg aaggcagttt acccagccag cctttacgca ccaccaggcc
     3661 tttatgaata cgcttactgg tacgtccgga gttcaacgct ttgtacagcg gctccaccgc
     3721 cgcaatctct tcatccgtcg cccgggtacg aatcgacata tagccactga tttttccctc
     3781 gcgcaccatc ggtaccgcat tggcccgcac ccaataatgg tcgccatttt tgcggcgatt
     3841 tttcacgatg ccgctccagg gctccccttt tttcagggtg aaccacatat ccgcaaacgc
     3901 cgcttttggc atatccgggt gacgcaccat gttgtgcggc tgcccttgta actcttgcaa
     3961 ggtaaagccg ctcacctgca caaaagtgtc attagcatga gtgatatagc tttgcagatc
     4021 ggtagtggac atcagagtgg tatcgtccgc cagcggggta ttttgctggg tgacatacgg
     4081 atgagaagac atgttcgcgt cctgtgcagg ttatatggtt gttaacttct tgtcagagtt
     4141 tatgtcggcc ccgctgcggt tatctttaac cgattaattt gatttagatc gcaatttgcg
     4201 attaaagcac aaatctaatt ctttgattta aaatactttc actctgttac tatacgaaaa
     4261 cgttaattat cttgcccaaa aatcaggcaa ttattgccct gaaaacgtgc atttgcgcag
     4321 caatcatcaa atccataccc gacaaaaacc gtgcaaaata acaacaaatg ttaacggata
     4381 gcattaaata ttgcacaaat gataaccgca tttgtgttta tcccgatttt cgcgatcgca
     4441 gccggagtgg cgcaatccct gcaatactta aatcggtatc atgtgatacg cgagcctccg
     4501 gagcatattt tgaacaggtt accttcgagc gcatcggctt tagcgtgcag cgcccacgcc
     4561 ctgaatctca ttgagaagcg aacgctggat catgaggaga tgaaagcact taaccgagag
     4621 gtgattgaat acttcaaaga gcatgtcaat ccggggtttt tagagtatcg caaatctgtt
     4681 accgccggcg gggattacgg agccgtagag tggcaagcgg gaggtttaaa tacgcttgtc
     4741 gacacccagg gacaggagtt tatcgactgc ctgggaggtt ttggaatttt caacgtgggg
     4801 caccgtaatc cagttgtggt ttccgccgta cagaatcaac ttgcgaaaca accactgcac
     4861 agccaggagc tactcgatcc gttacgggct atgctggcga aaacccttgc tgcgctaacg
     4921 cccggtaaac tgaaatacag cttcttctgt aatagcggca ccgagtccgt tgaagcggcg
     4981 ctgaagctgg cgaaggctta tcagtcgccg cgcggcaaat ttacttttat tgccaccagc
     5041 ggcgtgttcc acggtaaatc acttggcgcg ctgtcggcca cggcgaaatc gaccttccgc
     5101 aaaccgttta tgccactgct gccagggttc cgtcatgtgc cgtttggcaa tatcgaagcc
     5161 atgcgcacgg ctcttaatga gtgcaaaaaa accggtgatg atgtggctgc ggtgatcctc
     5221 gaaccgattc agggcgaagg tggcgtaatt ctgccgccgc cgggctatct caccgccgtg
     5281 cgtaagctgt gcgatgagtt cggcgcactg atgatcctcg atgaagtaca aacgggcatg
     5341 gggcgcacgg gcaagatgtt tgcctgcgaa catgagaacg ttcagccgga cattctgtgt
     5401 ctggcaaaag ccctcggcgg cggcgtgatg ccgattggcg cgaccatcgc cactgaagag
     5461 gtgttttcag ttctgttcga caacccattc ctgcatacca ccacctttgg cggcaacccg
     5521 ctggcctgtg cggcggcgct ggcgaccatc aatgtgttgc tggagcagaa cttaccggct
     5581 caggctgagc aaaaaggcga tatgttgctg gacggtttcc gtcaactggc gcgggaatat
     5641 cccgatctgg tacaggaagc gcgtggtaaa gggatgttga tggcgattga gtttgttgat
     5701 aacgaaatcg gctataactt tgccagcgag atgttccgcc agcgcgtact ggtggccgga
     5761 acgctcaata acgccaaaac gatccgcatt gaaccgccac tgacactgac cattgaacag
     5821 tgtgaactgg tgatcaaagc ggcgcgtaag gcgctggcgg ccatgcgagt aagtgtcgaa
     5881 gaagcgtaat atcaaatcgg atggcgatac gacgtcgcca tccgatcttt ttatacgacg
     5941 cgtacgcccg caggcatcat ccgctccggc gttaacaaca cgctttcact gccgtcatcg
     6001 gtttccgcac acaacagcat gcattccgac gtttcaccac gcattttcgc tttttgcaga
     6061 ttgcataaga ccaccaccgt tttccccatc agctcctctt cgctgtagta cggcaccagg
     6121 ctggtcacgg tttgcagtgt tttttcccca catcaacctg tacgatgtac agcttgtcgg
     6181 cgttttcatg gcgtttcact tccacaatct ttccgacgcg catttccaga cgtgcaaaat
     6241 cagcgtaagc cacggtttcc attgcttcct cccttcgagt aaaattttac taaactatag
     6301 aaaagttttt ctcaatcctg taggctaaaa atggagaatg caggcgtgat cacattccta
     6361 agccgctgtg ttaccgttac agcgtcaaag aaacgcgctt tatttactga aaacaggtga
     6421 cccgataagc acttcctcta caatgggggc gcacatcagg gaaagtaaaa aaggtaaaca
     6481 tggcaacatt aaaagacatc gcaatcgaag ctggcgtatc cctggcgaca gtatccaggg
     6541 tcttaaatga cgatccgaca ttgaatgtga aagaagagac gaaacatcgc attctcgaga
     6601 tcgccgaaaa gctggagtac aagaccagta gcgcccgtaa actacagaca ggcgcagtca
     6661 accaacacca tattctggct atctacagct accagcagga gctggagatc aacgatcctt
     6721 actatctggc gatccgccac ggcattgaaa ctcagtgcga aaagctggcg atcgaactga
     6781 ccaactgtta tgaacacaac ggattaccag acattaaaaa cgtcaccggt attttaattg
     6841 tcggcaaacc cacgcccgcc ctgcgcgccg ctgccagcgc gttgaccgac aatatctgtt
     6901 ttatcgactt tcacgaaccc ggcagcggtt acgatgcggt ggatatcgat ctggcacgca
     6961 tcagtaaaga aatcatcgac ttctatatca accagggcgt taatcgtatt ggttttattg
     7021 gcggtgaaga tgagcctggc aaggcggata ttcgtgaggt cgcctttgcg gaatatggcc
     7081 gactgaaaca agtggtacgc gaagaggata tctggcgcgg cggtttttcc agttcgtcgg
     7141 gttatgaact ggcaaaacaa atgctggcgc gggaagacta tccgaaggca ctgtttgttg
     7201 cttccgattc cattgctatc ggcgtactgc gggcaattca tgaacgtggc ctgaacatcc
     7261 cacaagatat ttcgcttatc agcgttaacg atatccccac cgcgcgattt acctttccgc
     7321 cgctctccac cgtgcgcatc cattccgaga tgatgggaag tcagggcgtt aacctggtgt
     7381 atgaaaaagc ccgcgatggt cgcgcgctgc cgctgctagt cttcgttccc agcaaattaa
     7441 aactgcgcgg cacgacccgt taaatcccct tacacactgt tcggcaatca tttttgccgg
     7501 acagtgctgc cgtttatttt cgtgatccag ttaaagtaaa tgcatttacc tgctgctttt
     7561 tagtaaaaat tttactaaac ccccagcaat tacacaaact accatcacca tgaatggttc
     7621 cgatttctct ctaccgggag gccctatgaa tcgctgggaa aacattcagc tcacccacga
     7681 aaaccgactt gcgccgcgtg cgtacttttt ttcatatgat tctgttgcgc aagcgcgtac
     7741 ctttgcccgc gaaaccagca gcctgtttct gcccttaagc agtcagtgga atttccactt
     7801 ttttgaccat ccgctgcaag taccagaagc cttcacctct gagttaatgg ctgactgggg
     7861 gcatattacc gtccccgcca tgtggcaaat ggaaggtcac ggcaaactgc aatataccga
     7921 cgaaggtttt ccgttcccca tcgatgtgcc gtttgtcccc agcgataacc caaccggtgc
     7981 ctatcaacgt attttcaccc tcagcgacgg ctggcagggt aaacagacgc tgattaaatt
     8041 tgacggcgtc gaaacctatt ttgaagtcta cgttaacggt cagtatgtgg gtttcagcaa
     8101 gggcagtcgc ctgaccgcag agtttgacat cagcgcgatg gttaaaaccg gcgacaacct
     8161 gttgtgtgtg cgcgtgatgc agtgggcgga ctctacctac gtggaagacc aggatatgtg
     8221 gtggtcggcg gggatcttcc gcgatgttta tctggtcgga aaacacctaa cgcatattaa
     8281 cgatttcact gtgcgtaccg actttgacga agcctattgc gatgccacgc tttcctgcga
     8341 agtggtgctg gaaaatctcg ccgcctcccc tgtcgtcacg acgctggaat ataccctgtt
     8401 tgatggcgaa cgcgtggtgc acagcagcgc cattgatcat ttggcaattg aaaaactgac
     8461 cagcgccagc tttgctttta ctgtcgaaca gccgcagcaa tggtcagcag aatcccctta
     8521 tctttaccat ctggtcatga cgctgaaaga cgccaacggc aacgttctgg aagtggtgcc
     8581 acaacgcgtt ggcttccgtg atatcaaagt gcgcgacggt ctgttctgga tcaataaccg
     8641 ttatgtgatg ctgcacggcg tcaaccgtca cgacaacgat catcgcaaag gccgcgccgt
     8701 tggaatggat cgcgtcgaga aagatctcca gttgatgaag cagcacaata tcaactccgt
     8761 gcgtaccgct cactacccga acgatccgcg tttttacgaa ctgtgtgata tctacggcct
     8821 gtttgtgatg gcggaaaccg acgtcgaatc gcacggcttt gctaatgtcg gcgatatcag
     8881 ccgtattacc gacgatccgc agtgggaaaa agtctacgtc gagcgcattg ttcgccatat
     8941 ccacgcacag aaaaaccatc cgtcgatcat catctggtcg ctgggcaatg aatccggcta
     9001 tggctgtaac atccgcgcga tgtaccatgc ggcgaaagcg ctggatgaca cgcgactggt
     9061 tcattacgaa gaagatcgcg atgctgaagt ggtcgatatt atttccacca tgtacacccg
     9121 cgtgccgctg atgaatgagt ttggtgaata cccgcatccg aagccgcgca tcatctgtga
     9181 atatgctcat gcgatgggga acggaccggg cgggctgacg gagtaccaga acgtcttcta
     9241 taagcacgat tgcattcagg gtcattatgt ctgggagtgg tgcgaccacg ggatccaggc
     9301 acaggacgac cacggcaatg tctggtataa attcggcggc gactacggcg actatcccaa
     9361 caactataac ttctgtcttg atggtttgat ctattccgat cagacgccgg gatcgggcct
     9421 gaaagagtac aaacaggtta tcgcgccggt aaaaatccac gcgctgactc gcggcgagtt
     9481 gaaagtcgaa aataaactgt ggtttaccac gcttgatgac tacaccctgc acgcagaggt
     9541 gcgcgccgaa ggtgaaacgc tcgcgacgca gcagattaaa ctgcgcgacg ttgcgccgaa
     9601 cagcgaagcc cccttgcaga tcacgctgcc gcagctggac gcccgcgaag cgttcctcaa
     9661 cattacggtg accaaagatt cccgcacccg ctacagcgaa gccgggcatt ctatcgccac
     9721 ttatcagttc ccgctgaagg aaaacaccgc gcagccagtg cctttcgcac caaataatgc
     9781 gcgtccgctg acgctggaag acgatcgttt gagctgcacc gttcgcggct acaacttcgc
     9841 gatcaccttc tcaaaaatga gtggcaaacc gacatcctgg caggtaaatg gcgagtcgct
     9901 gctgacccgc gagccaaaga tcaacttctt caagccgatg atcgacaacc acaagcagga
     9961 gtacgaaggg ttgtggcagc cgaatcattt gcagatcatg caggaacatc tgcgcgactt
    10021 tgccgtagaa cagagcgatg gtgaagtgtt gatcatcagc cgcacagtta ttgcaccgcc
    10081 ggtgtttgac ttcgggatgc gctgtaccta catctggcgc atcgctgccg atggccaggt
    10141 taacgtggcg ctttccggcg agcgttacgg cgactatccg cacatcattc cgtgcatcgg
    10201 tttcaccatg gggattaacg gcgaatacga tcaggtggcg tattacggtc gtggaccggg
    10261 cgaaaactac gccgacagcc agcaggctaa catcatcgat atctggcgca gcaccgtcga
    10321 tgccatgttc gagaactatc ccttcccgca gaacaacggc aaccgtcagc atgtccgctg
    10381 gacggcactg actaaccgcc acggcaacgg tctgctggtg gttccgcagc gcccaattaa
    10441 cttcagcgcc tggcactata cccaggaaaa catccacgct gcccagcact gtaacgagct
    10501 gcagcgcagt gatgacatca ccctgaacct cgaccaccag ctacttggcc tcggctccaa
    10561 ctcctggggc agcgaggtgc tggactcctg gcgcgtctgg ttccgtgact tcagctacgg
    10621 ctttacgttg ctgccggttt ctggcggaga agctaccgcg caaagcctgg cgtcgtatga
    10681 gttcggcgca gggttctttt ccacgaattt gcacagcgag aataagcaat gaggatcatc
    10741 gataacttag aacagttccg ccagatttac gcctctggca agaagtggca acgctgcgtt
    10801 gaagcgattg aaaatatcga caacattcag cctggcgtcg cccactccat cggtgattca
    10861 ttgacctacc gtgtggaaaa tgactccgcg accgatgcgc tatttaccgg gcatcgacgc
    10921 tattttgaag tgcattacta cctgcaaggg cagcaaaaaa ttgaatatgc gccgaaagag
    10981 acattacagg tagtggaata ttatcgtgat gaaactgacc gtgaatattt aaaaggctgc
    11041 ggagaaaccg tagaggtcca cgaagggcaa atcgttattt gcgatatcca tgaagcgtat
    11101 cggtttatct gcaataacgc ggtcaaaaaa gtggttctca aagtcaccat cgaagatggt
    11161 tatttccata acaaataaca actacggcgg caaaaggagt ttgccgccac cgctacccta
    11221 cacattttcg gagatgtgtt atgtctgata ccaaacgtaa tacaatcggc aaattcggct
    11281 tgctctcgct gacttttgcc gccgtttaca gctttaacaa cgttatcaat aataatattg
    11341 agcttggact ggcctcggca ccgatgtttt tcctcgcgac gattttttat tttattccct
    11401 tctgtctgat catcgcagaa tttgtttcgt taaataaaaa ctcagaagcc ggtgtctacg
    11461 cgtgggtaaa aagttcgctg ggcggacgtt gggcatttat tactgcctac acttactggt
    11521 tcgtaaatct gttctttttc acctcgctgt tgccgcgcgt tattgcttat gcttcgtatg
    11581 ccttcctcgg ctacgaatat attatgacgc cggttgccac caccgttatc agtatggtgc
    11641 tgttcgcctt ctccacctgg gtttccacca acggggcgaa aatgctgggg ccaattacct
    11701 ccgtcacttc aacgctgatg ctgctgttaa cgctctccta cattttactg gcaggtacgg
    11761 cactggttgg cggcgtacag cctgccgatc cgatcaccgt tgacgcgatg atcccgaact
    11821 tcaactgggc gttcctcggc gttaccacct ggatctttat ggccgcaggt ggcgcggagt
    11881 ccgtcgcggt gtacgttaac gacgtcaaag gcggttcgaa atcgttcgtt aaagtgatca
    11941 tcctcgccgg gatttttatc ggcgtactgt attccgtctc ctcggtgctg attaacgtct
    12001 tcgtcagcag caaagagttg aaatttaccg gcggatcggt gcaggtattc cacggcatgg
    12061 cggcgtattt tggtctgccg gaagcgttga tgaatcgctt tgtcggtctg gtgtccttta
    12121 ccgcaatgtt cggttccctg ctgatgtgga ccgcaacgcc ggtgaaaatt ttcttctccg
    12181 aaatcccgga aggcatcttt ggtaagaaaa ccgtcgaact taacgaaaac ggcgttccgg
    12241 cgcgcgcagc atggatccag tttctgatcg tcatcccgct gatgattatc ccgatgctcg
    12301 gttccaatac cgtgcaggat ctgatgaata ccattattaa tatgaccgcc gcagcgtcca
    12361 tgcttccgcc gttattcatc atgctggctt acctgaattt acgcgccaaa ttagatcacc
    12421 tgccacgcga tttccgtatg ggctcccgcc gcaccggtat tatcgttgtt tcaatgctga
    12481 ttgcgatatt tgccgtaggg tttgtcgctt cgacattccc gactggcgcg aatattctga
    12541 ccatcatttt ttataacgtc ggcggtattg ttatcttcct cggctttgcg tggtggaaat
    12601 acagcaaata tataaaggga ttaacggctg aagagcgcca tattgaagcg acgccagcca
    12661 gcaatgttga ttaagcagac taataaaaaa tgagcaggca ttttccctct tcctggatga
    12721 ggagagggaa aatagtttct gcctttatat ttttataatt acaacgataa aaggctgtac
    12781 tttttcttta gctcatggat taacacaatg aaattaatca ctgcaccatg cagagcatta
    12841 cttgctctgc cgttttgcta cgccttttct gcggcaggag aagaagcacg tccggcagaa
    12901 catgacgaca caaaaacacc cgcaattacc tcgacatctt ctccttcatt tcgtttttac
    12961 ggcgaattag ggcttggtgg atatatggat ttagagggtg agaataaaca taaatacagc
    13021 gacggcacct atattgaagg tggcctggag atgaagtacg gctcctggtt cggcctgatt
    13081 tacggcgaag gctggaccgt gcaggccgac cacgacggta atgcctaggt gcctgaccat
    13141 agctggggtg gtttcgaagg tgggattaac cgtttctatg gcggttatcg taccaatgat
    13201 ggcaccgaaa tcatgctcag tctgcgtcag gattcctcgc tggatgacct gcaatggtgg
    13261 ggcgacttca cacccgattt gggctacgtc attcccaata cccgcgacat tatgaccgcg
    13321 ctgaaggtac agaacttaag cggcaacttt cgttatagcg tcaccgccac acctgccgga
    13381 catcatgacg aaagcaaagc ctggctacat tttggcaagt acgatcgcta cgacgacaaa
    13441 tacacctatc cggcaatgga tgaacggtta catccagtat gaccttgccg aaggcatcac
    13501 ctggatgaat ggtctggaaa ttaccgacgg cacaggacaa ctctatctca cgggcctgtt
    13561 tacccctaac tttgccgctc gcgcctggca ccataccgga cgtgccgacg ggctggacgt
    13621 accgggaagt gaaagcggga tgatggtgag cgccatgtat gaagcgttaa aaggcgttta
    13681 tctctccacc gcttacacct acgccaaaca tcgccctgac cacgctgacg atgaaaccac
    13741 ctctttcatg cagtttggta tctggtacga atatggcggc ggacgtttcg ccacggcttt
    13801 tgatagccgc ttctacatga aaaatgcctc taacgatccc agcgaccaaa tcttcctgat
    13861 gcaatatttc tactggtaat aaggactgta atctcatgaa aatcaaaact attttaacgc
    13921 cagtaacctg cgctctgctg ataagttttt ccgctcatgc cgctaacgcc gacaattata
    13981 aaaacgtgat taaccgtact ggtgcgccgc agtacatgaa ggattacgat tacgacgatc
    14041 accagcgttt taatccgttt ttcgatctcg gagcctggca tggtcatctg ttgccagacg
    14101 gcccgaacac catgggcggc tttccgggcg ttgcgctgct gacggaagaa tacatcaact
    14161 ttatggccag caatttcgac cgcctgactg tctggcaaga cggcaagaaa gtcaacttca
    14221 cgctggaggc atacagtatt cccggcgcgc tggtgcaaaa actgacagca aaagatgtgc
    14281 aggtcgaaat gactctgcgc ttcgccacgc cgcgcacgtc actactggaa actaaaatca
    14341 tcagcaataa accgctggat ctggtgtggg atggcgaact gctggaaaaa ctggaagcga
    14401 aagaagggaa accgctttcc gataaaacca ttgctggcga ataccctgac tatcagcgca
    14461 aaatcagcgc cacccgtgat ggcctgaaag tcacctttgg caaagtgcgc gccacctggg
    14521 atctgctgac ctccggcgaa tcggaatatc aggtgcataa atccctgccg gtgcagactg
    14581 aaatcaacgg taatcgcttt accagtaagg cgcatatcaa cggttcgacc acgctctaca
    14641 ccacctattc ccatctgctg accgctcagg aagttagcaa agagcaaatg cagatccgcg
    14701 atattctggc gcgtccggcg ttttatctca ccgcctcgca gcaacgctgg gaagaatacc
    14761 tgaagaaagg gttaaccaat ccggatgcga cgcaggaaca gacgcgcgtc gcggtgaaag
    14821 cgatcgaaac gctcaacggt aactggcgct caccgggcgg tgcggtgaat tataacaccg
    14881 tcacgccgtc ggtgaccgga cgctggttct ccggcaatca gacctggccg tgggatacct
    14941 ggaagcaggc gtttgcgatg gcgcatttca atccggatat cgccaaagag aatatccgcg
    15001 cggtcttctc ctggcagatc cagcctggcg acagcgtgcg tccgcaggat gtgggctttg
    15061 tccccgacct gatagcgtgg aaccttagcc ccgagcgtgg tggcgatggc ggcaactgga
    15121 acgaacgtaa taccaaaccc agccttgccg cctggtcggt gatggaagta tacaacgtca
    15181 cccaggataa agcctggttg gcagagatgt acccgaaact ggtggcctat cacgactggt
    15241 ggttacgtaa ccgcgatcat aacggcaacg gggtgccgga atatggcgcg acccgcgaca
    15301 aagcccacaa caccgagagc ggcgagatgc tgtttacggt gaaaaaaggc gataaagaag
    15361 agacgcagtc tgggctgaac aactacgccc gcgtggtgga gaaaggccag tacgacagtc
    15421 tggaaattcc ggcacaggtc gctgcgtcgt gggaatcggg gcgtgatgat gccgccgtct
    15481 ttggttttat cgacaaagaa cagttggata aatatgtcgc taatggcggc aaacgtagcg
    15541 actggacggt gaaattcgcc gaaaaccgca gtcaggacgg aacgttgctg ggctactcgc
    15601 tattgcagga gtcggtggat caggccagct atatgtacag cgataaccat tatctggcgg
    15661 agatggcaac catcctcggt aagccagaag aggccaaacg ctatcgccag ttggcacagc
    15721 agctcgcgga ctacatcaac acctgtatgt tcgacccgac tacccagttc tactatgacg
    15781 tacgtattga agataaacca ctggcgaacg gctgcgcggg caaaccgatt gttgagcgcg
    15841 gtaaagggcc ggaaggctgg tcgccgctgt ttaacggtgc ggcaacgcag gccaatgccg
    15901 acgcggtggt gaaggtgatg ctcgatccta aagagttcaa cacatttgtt ccgctgggaa
    15961 cggcggcgtt aaccaatccg gcttttggcg ctgatatcta ctggcgcggg cgcgtatggg
    16021 tggatcagtt ctggtttggt ctgaaaggga tggagcgtta cggttatcgt gatgatgccc
    16081 tgaagctggc ggatacgttc ttcaggcacg ccaaaggatt aaccgccgat ggcccaattc
    16141 aggagaatta caacccgctg acaggcgcgc agcaaggcgc accaaatttc tcctggagtg
    16201 ccgctcattt gtatatgttg tataacgatt ttttccgtaa gcagtaattt gcaacggctg
    16261 gcggatgcgg cgtgaacgcc ttatccgccc tacatgtgca atcccccaag ccggataaaa
    16321 cacggcaagc gtcgcatccg gcgttaccac cgggcgtatt ctttttgaat cccatcacaa
    16381 accccgcact ccccttttcc cttttctcca tcgacggcta aattagaact catccgacta
    16441 cataacaatt attttacata ctggacaatt ttatgagcta cccgtcgctg ttcgccccgc
    16501 tggatttagg ttttaccacg ttaaaaaacc gcgtgttgat gggttcaatg cacaccgggc
    16561 tggaggaata cccggacggt gctgaacggc tggctgcgtt ttatgccgaa cgcgcccgtc
    16621 acggcgtggc gctgattgtc agcggcggca ttgcaccaga tttaacaggc gttggcatgg
    16681 aaggtggcgc aatgctcaac gacgccagcc agatcccaca ccatcgcacc attaccgaag
    16741 ccgtacatca ggaaggcggc aaaatcgccc tgcaaatttt gcataccggg cgctacagct
    16801 accaaccgca tctggtcgca ccgtccgcat tgcaggcccc catcaaccgt ttcgttcctc
    16861 acgaactcac ccatgaagag atcctgcaac tgatcgacga tttcgcccgc tgcgcgcaac
    16921 tggcgcggga ggccggatac gatggcgtcg aagtgatggg ctctgaaggg tatttgatca
    16981 acgaatttct gacgctgcgc accaatcagc gtagtgacca gtggggcggc gattaccgca
    17041 accggatgcg atttgccgta gaagtagtgc gtacggtgcg cgaacgcgtc ggcaacgact
    17101 tcattattat ttaccggttg tcgatgctcg acctagtcga aggcggcggc acttttgccg
    17161 aaacggtaga actggcgcag gccattgaag cggcgggcgc gaccattatc aacaccggaa
    17221 taggctggca cgaagcgcgg attccgacca tcgccacgcc cgtgccgcgc ggcgcattta
    17281 gctgggtcac gcgcaaactg aaaggccacg tctcgctgcc gctggtaacc accaaccgga
    17341 ttaacgatcc gcaggttgcc gacgatattc tctcgcgcgg cgatgccgat atagtatcga
    17401 tggcgcgacc gtttctcgcg gatgcggaac tgctgtcaaa agcgcaatca gggcgagccg
    17461 atgagatcaa cacttgtatt ggctgcaacc agggctgcct cgatcagatc ttcgttggca
    17521 aagtcacctc gtgcctggtg aatcctcgcg cctgccacga aaccaaaatg ccaatccttc
    17581 ccgccgtgca gaaaaaaaat ctggcggtgg tcggcgctgg acctgctggg ctggcgtttg
    17641 ccattaacgc ggcggcgcgt gggcatcagg taacattgtt tgatgcgcac agcgagatcg
    17701 gcgggcagtt taatatcgcc aaacagatcc ccggcaaaga ggagttttac gaaacgctgc
    17761 gctactaccg ccggatgatc gaagtgacgg gcgtgacgct gaaactcaat cacaccgtga
    17821 cggcggatca gttacaggcg ttcgatgaaa cgatcctcgc cagtgggatc gtgccgcgtg
    17881 ttccgcctat cgacgggatc gatcatccga aggtattgag ttatctcgat gtactgcgcg
    17941 acaaagcgcc ggtaggcaac aaggttgcca tcatcggttg tggcgggatt ggttttgata
    18001 cggcgatgta tttaagtcag ccgggcgaat ccaccagcca gaatatcgcc aggttctgta
    18061 atgagtgggg gatcgacagc agcctacaac aggctggtgg cttaagcccg cagggaatgc
    18121 agatcccgcg tagcccacgg caaattgtga tgctccagcg caaagccagc aaaccgggac
    18181 aggggttagg caaaaccacc ggctggatcc atcgcaccac cctgctctcg cggggtgtga
    18241 aaatgatccc aggcgtaagt tatcagaaga ttgacgatga cgggctgcat gtggtgatca
    18301 acggcgaaac gcaggtatta gcagtggaca atgtggtgat ctgcgcaggg caagagccaa
    18361 accgcgcgct ggcgcaaccg ctgattgata gcgggaaaac tgtgcattta attggcggct
    18421 gcgatgtggc tatggagctg gacgcacgac gggcaattgc ccagggaaca cggctggcgc
    18481 tggagattta aatcgttatt tgaagcgccg gatgcaacgc attcggcacg tcgcgttaat
    18541 caataaacaa ggcgggagaa atgccgaatc gcgttgccaa ttttttagcg tgttccagcg
    18601 ttaatttcct cttcccgctc aaaacgcgtg acaccataga tttactgcca atttccggca
    18661 gatcggaaag ggttaaacca tattgatcca taagggtacg aatcacggct ataccgccag
    18721 gcatggcttg agccatggca ttaaattccg caaattcggg cgctgattct tcccacgcgg
    18781 ttattttggc acacaccaga tccagcaagg ggttttcagg atcgttgagc agcagatgat
    18841 ctaccagttc cagcgcctgg gtgtattgtt cctcgttctg aatacccgcc agaaaaggtg
    18901 ccacagcagt tagcttttct cctgcttgca agatgtcggc aatcgcaatc attttttccc
    18961 cttagtacga tgaacagcgg taaagaaatc gtattcttta tgcgtcataa cttcacgtat
    19021 gtagcacttt tgcgattcaa aaaagaccat tgctacaaca cgtaattcat tgcccccaac
    19081 attgaaaaca taatgcttat ccagatattt gaagttatcc agagatggga atactgcttt
    19141 taatgactca ggttttttga aatatccctt agcaatcgtg ttccccagag ccaccaactc
    19201 cgttttatgt tgcgggtatt tttccgcagc atctttcaat gctttttgag ttatcaggtg
    19261 cattcttcat cacgtccgtt gacaaattgg caatatgata acatccgttg acagattggc
    19321 aacggatgaa tttatctgtg tgacggaaga ataaacgtag aaaaacgcta aacaagagaa
    19381 tgtagaggat ggtgacaggg attttttgtt ttatggaagc gcgattccag aactgagcag
    19441 acattgccgg atgcgacgct tgccgcgtct tatccggcct gggggtaacg tgctgaacgt
    19501 aggccggata aggcgtttac gccgcatccg gcaggtaact cccaatctta ccgacgacgt
    19561 cccagcttca ccgctttcag caccacaaat ttattattcg tcgcaatagt ggtgcagttg
    19621 ccgaaaattt tcttcagttt atggaagtaa tccagatggc ggttggcaac gatatacagc
    19681 tcgccgttga ttttcaggca gcggcgggcg tagtggaaca tctcccaggc gacgttatcg
    19741 gtcagcgcat gttgttggtg aaacggaggg ttgcagagca cagcattaaa gcggaaaggc
    19801 tccacgccgg agagcgcgtt gttgatcata aactcgctgc gatccaacgc ctctggcatg
    19861 ttggtttcaa cgttcaaacg gctggaagca accgccatcg gcgattcatc gacaaacacc
    19921 actttcgcct gcgggttttt atcaagcagc gtcagaccaa taacgccatt accgcaaccg
    19981 agatcgacaa tctccccttc gagattctct ggcagatgtt gcataaagaa gcgcgcgccg
    20041 atatcaagcc cggtgcggga gaagacattc gcatggttat ggatagtcca gtcagtacct
    20101 tccagcttcc agctaacggt ctgcggcgca tcggccagcg gcggttcatt gaacgtgcaa
    20161 ttaatcaggc gcgctttttt ccatgccagc gtggtggtgg tcgggccgag cactttttcg
    20221 aacagttcca gcgtggaagt gtgaatgtca cgggctttgg caccggcaat aatacgtgta
    20281 tccgacgtga ccactttgcg cagcgcacgc agttgctgtt ccagcaatgc cagtgttttc
    20341 ggcactttga tcagcaccac acccggctgt tgcgggtagt cggcggtgct gtcgagaaac
    20401 ttcacgctcg actcatcaat cccgtttagg cgtaaattct cgcgcgtcgc cagttcactg
    20461 atgtatgagt cgccaatgct gtacggctta tgttccgcca gcgcgcagct taacgcacca
    20521 aaggcatcat tcaggatcaa caccgggccg aggatttctg tgtcgtccaa ctgttgcagc
    20581 aaatattcat ctgccgcttc ccacgcctgt agcgggttaa cgtcatccgt cgccggaaaa
    20641 cgttgtagtg tcagtgaacg gaaaccgttg tctaagtggc tcatcggccc tcctgaatga
    20701 taaaatttcg acgttgcccc tgaaaagggt gcgtgagtat accctttttc tctttttcgt
    20761 gggcggttat gagcaatctt acttatcttc agggttatcc cgagcagctg ctttcgcagg
    20821 tgcgaacact gattaacgaa cagcgtctgg gcgatgtgct ggcaaaacgc tatccgggaa
    20881 cacacgacta cgccaccgat aaagctctct ggcaatatac tcaggatctg aaaaatcagt
    20941 ttctgcgtaa tgccccgccg atcaataaag tgatgtatga caataagatc catgtgctaa
    21001 aaaacgcgct cgggttacat accgctgttt ctcgtgtgca gggcagcaag ctgaaagcaa
    21061 aagcagagat ccgcgtcgct accgtgtttc gcaacgcgcc ggaaccgttt ttgcgcatga
    21121 tcgtggtgca cgagctggcg cacctgaagg agaaagagca taacaaagcg ttctatcaat
    21181 tgtgctgcca tatggagccg cagtaccacc agcttgagtt cgacacccgg ctgtggctaa
    21241 cgcagttgtc gcttgggcag gacaaaatct gaaaatgatg tcaggcgtta tcttttcttt
    21301 ctttagcgcc taaaatcgac ctcccccctt tcgttaagga taacgaccat gctgcgcgca
    21361 tttgcccgcc ttcttctccg tatttgtttt tctcgccgga ctctaaagat tgcctgctta
    21421 ctgttacttg tggcaggagc gaccattttt atcgccgatc gcgttatggt caacgccagc
    21481 aagcagctga cctggggcga tgtcaacgcc gtcccggcgc gtaacgtggg tttattgctg
    21541 ggggcgaggc cgggtaatcg ctactttacc cgacgtattg ataccgcagc agcgttgtac
    21601 cacgccggaa aagtgaaatg gctactggtg agtggcgata acggacgtaa aaattacgat
    21661 gaagcatcag gtatgcagca ggctttgatc gctaaaggcg taccggcgaa agtgatcttt
    21721 tgcgactatg ccggattctc aacgctggat tcggtagtgc gtgccaaaaa ggtgtttggg
    21781 gaaaaccata tcaccattat ctcgcaagaa tttcataacc aacgcaccat ctggctggca
    21841 aaacagtacg gcatcgacgc tatcgggttt aacgcgccag acctcaatat gaagcacggt
    21901 ttttatactc aactgcggga gaaactcgct cgtgtcagcg cggtgattga cgcaaaaatc
    21961 ctccatcgcc agccgaaata tctcgggcca tcggtcatga ttgggccgtt tagtgagcat
    22021 ggctgtccgg cgaaagaata atgcgtatct gcgcacgtcg aagatgaaaa aggcgtgcta
    22081 cattgacgac agaatccctt tatggagtat ccacgcgtta tgatacgttt cgctgtgatt
    22141 ggtacgaact ggatcactcg ccagttcgtc gaggccgccc atgagagcgg taaatacaag
    22201 ttaaccgccg tatattcccg cagccttgag caggctcagc acttcgccaa tgatttttct
    22261 gtcgagcatc tgtttacgtc gctggaagcg atggcggaaa gcgatgccat tgacgcggtg
    22321 tatattgcca gcccgaattc cctgcacttt tcccagacac aacttttcct tagccataaa
    22381 attcatgtga tttgcgaaaa accgctggcg tcgaatctgg cggaagtgga tgccgccatt
    22441 gcctgtgcgc gcgaaaatca ggtggtgctg tttgaggcat ttaaaaccgc ctgcctgccg
    22501 aactttcatt tgttgcgcca ggcgctgccg aaagcaggca aactgcgtaa agtctttttc
    22561 aactattgcc agtattcctc gcggtatcaa cgttacctgg acggtgagaa tcccaacacc
    22621 tttaatccgg cattctctaa cggttcaatt atggatatcg gcttttactg cctggcgtcg
    22681 gcggtggcgt tatttggtga gccgaaaagc gtgcaggcaa cggcgagttt gctggcaagc
    22741 ggcgttgacg cccacggcgt ggtggtgatg gattacggtg atttcagcgt caccttgcag
    22801 cactcaaagg tcagtgattc tgtcctggga agcgagattc agggcgaagc gggatcgctg
    22861 gtgattgaaa aactgtctga atgccagaaa gtctgctttg tgccgcgtgg cagccagatg
    22921 caggatctca cccagccgca gcatattaat accatgctct acgaagcaga gctgttcgct
    22981 accctggtgg atgagcatct ggtggatcat ccagggctgg cggtcagtcg catcacagcc
    23041 aaactgctga ccgagatccg ccgccaaact ggggtgattt ttccggcaga tagcgtaaaa
    23101 ctatacttgc caaagtaaaa cagtgtaaaa ggtatgtaac agaccattga ctgactgaat
    23161 ggtctgtcat actttgttac ctgcaaaggg gagtaacttc attgccggtc gatcgtcatt
    23221 acgatgagtg aaaaaccaca tccggtcacc gggcaacccg aaaggaatac gcagacgtat
    23281 tccttttttg ttgtaagtga gaccttgccg gaaggcgagg tctatgcata aaaagcagcg
    23341 gctgacgtct tccgacgttg gccgtttttt tatgtgtaag gaacttctat gaatactgtc
    23401 ggcacgccgt tgctatgggg cggattcgct gttgttgtcg ccattatgct ggctatcgac
    23461 ctgttgttgc aggggcgtcg tggggcacat gccatgacca tgaaacaggc tgcggcctgg
    23521 tcgctggtct gggtgacgct gtcgttactg tttaacgccg ctttctggtg gtatctggtg
    23581 caaaccgaag gtcgcgccgt tgccgatcca caggccctgg cctttctcac gggttatctg
    23641 attgagaaat cgctggcggt cgataacgtc tttgtctggc tgatgttgtt cagctatttc
    23701 tccgttccgg cggcattaca acgtcgcgtg ctggtgtatg gcgtgctcgg ggcgattgtt
    23761 ctgcgtacca tcatgatctt cacaggcagc tggctgattt cgcagttcga ctggatcctg
    23821 tatatcttcg gtgccttcct gctgtttacc ggcgtgaaga tggcgctcgc ccatgaagat
    23881 gaatcaggca ttggcgacaa gccgctggtg cgctggctac gcggtcattt gcgcatgacc
    23941 gacaccatcg acaacgagca tttctttgtg cgtaagaatg gattgctgta cgccacaccg
    24001 ctgatgctgg tactgattct ggtggaattg agcgacgtga tttttgccgt ggatagcatt
    24061 ccggctatct tcgccgtgac cactgacccg ttcattgtgc tgacctcaaa cctgtttgca
    24121 atcctcggcc tgcgtgcgat gtatttcctg ctggcgggcg tagcagagcg tttctcgatg
    24181 ctcaaatatg gcctggcggt gattctggtg tttatcggta tcaagatgct gattgtcgac
    24241 ttctaccata ttccaatcgc cgtctcgctg ggcgtggtgt ttggcattct ggtgatgacg
    24301 tttattatca acgcctgggt gaattatcgg catgataagc agcggggaga atgattcgct
    24361 gtatattaag ccattcctag agataactca ttccagacaa aggtgagcta ttaattatgt
    24421 aatcggctta tagctcttta caaaaagacc gcacgggtgt tggtggcgtc aaaatcgccg
    24481 aagcgttccc tggaggccgg ggcgagggcg catcttacag ggacgttacc ttgcgcccga
    24541 cccggtagcc gcaagggata ggctgaggga acc
//
LOCUS       DAZOYF010000049        24181 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZOYF010000049 DAZOYF010000000
VERSION     DAZOYF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.049"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(22..594)
                     /locus_tag="ACHUOF_001836"
     CDS             complement(22..594)
                     /locus_tag="ACHUOF_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR0611032.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="ACHUOF_001837"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="ACHUOF_001837"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR0611033.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="ACHUOF_001838"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="ACHUOF_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR0611034.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="ACHUOF_001839"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="ACHUOF_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR0611035.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="ACHUOF_001840"
     CDS             complement(3556..5337)
                     /locus_tag="ACHUOF_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0611036.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="ACHUOF_001841"
     CDS             complement(5330..7102)
                     /locus_tag="ACHUOF_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0611037.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="ACHUOF_001842"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="ACHUOF_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR0611038.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="ACHUOF_001843"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="ACHUOF_001843"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR0611039.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10354
                     /locus_tag="ACHUOF_001844"
                     /pseudo
     CDS             8662..10354
                     /locus_tag="ACHUOF_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10419..11114
                     /gene="queC"
                     /locus_tag="ACHUOF_001845"
     CDS             10419..11114
                     /gene="queC"
                     /locus_tag="ACHUOF_001845"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR0611040.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="ACHUOF_001846"
     CDS             complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="ACHUOF_001846"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR0611041.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11658..12029)
                     /locus_tag="ACHUOF_001847"
     CDS             complement(11658..12029)
                     /locus_tag="ACHUOF_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR0611042.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="ACHUOF_001848"
     CDS             complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="ACHUOF_001848"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0611043.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DVASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14243..14533)
                     /gene="hupB"
                     /locus_tag="ACHUOF_001849"
     CDS             complement(14243..14533)
                     /gene="hupB"
                     /locus_tag="ACHUOF_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR0611044.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14724..17078)
                     /gene="lon"
                     /locus_tag="ACHUOF_001850"
     CDS             complement(14724..17078)
                     /gene="lon"
                     /locus_tag="ACHUOF_001850"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR0611045.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="ACHUOF_001851"
     CDS             complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="ACHUOF_001851"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR0611046.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="ACHUOF_001852"
     CDS             complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="ACHUOF_001852"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR0611047.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19536..20834)
                     /gene="tig"
                     /locus_tag="ACHUOF_001853"
     CDS             complement(19536..20834)
                     /gene="tig"
                     /locus_tag="ACHUOF_001853"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR0611048.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="ACHUOF_001854"
     CDS             complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="ACHUOF_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR0611049.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21800..22378
                     /locus_tag="ACHUOF_001855"
     CDS             21800..22378
                     /locus_tag="ACHUOF_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0611050.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22422..23897
                     /gene="ampG"
                     /locus_tag="ACHUOF_001856"
     CDS             22422..23897
                     /gene="ampG"
                     /locus_tag="ACHUOF_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR0611051.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5667 a         6664 c         5826 g         6024 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacagtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catggcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccgcaaa ccgtcaccgt cagcgaactg gccgaacgct gtttttgcag
     8761 cgaacgccat gttcgtacgc tgttgcgtca ggcacaggag gcgggatggc tggagtggca
     8821 ggcgcagtca ggacgcggaa agcgcggaca attacgcttt ctggtcacgc cggaatcgct
     8881 acgcaatgcg atgatggaac aggcactgga aaccggaaag cagcaagatg tgctggagct
     8941 ggcgcaactg gccccaggtg agctgcgcac tctgttacag ccatttatgg gcggacaatg
     9001 gcaaaacgat acacccacgt tgcgtattcc ctactatcgc ccgctcgaac cgctacaacc
     9061 aggctttttg cccggccgtg ccgagcagca tctcgccggg cagatatttt ccggcctgac
     9121 ccgcttcgat aataatactc agcgcccgat tggcgattta gcgcatcact gggaaacctc
     9181 tactgacagg ttacgctggg acttttatct tcgttcaacc ctacactggc ataacggcga
     9241 tgcagtaaaa gcctcacact tacaccagcg attattgatg ctgttacaac tgccagcact
     9301 ggatcaatta tttattagcg tgaagcgtat tgaagtcacc catccgcagt gtctgacctt
     9361 ctttttacat cgccccgatt actggcttgc gcaccggctg gcgagctatt gcagccatct
     9421 ggcgcatccg caattcccac tgatcggcac tggtcctttt cgcttaacac aattcacagc
     9481 ggagctggtg cgcctggaga gccatgatta ttaccatttg cgtcatccgc tgcttaaagc
     9541 ggttgagtac tggataactc cgccgctttt cgaaaaagat ttgggaacca gttgtcggca
     9601 tcccgtgcaa atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc aagtcagtag
     9661 tggcatcagt ttaggttttt gctatttaac gttgcgcaaa agtccccgac tctccctctg
     9721 gcaggcgcga aaagtgatct ccattattca tcaatccggt ttattacaaa cgttagaagt
     9781 cggagaaaac ctgatcaccg ccagtcatgc attactgcca ggctggacta ttccgcactg
     9841 gcaagtaccg gatgaagtca aactaccgaa aaccttgacg ctggtttatc acctaccgat
     9901 agaacttcat accatggcag aacgcctaca ggcgacactg gcagcggaag gctgtgaact
     9961 cacaattatt tttcataacg caaaaaactg ggacgacacg accctactgg cacacgcaga
    10021 cctcatgatg ggcgacagat taattggcga agcaccggaa tatactctgg agcaatggct
    10081 acgttgcgat ccactatggc cacatgtttt cgacgctcca gcatatgcac atctgcaatc
    10141 gacgctggac gcggtgcaag taatgcctga tgaggaaaac cgatttaatg ccctgaaagc
    10201 ggtttttagc cagttaatgg cagacgcgac gctgacgccg ctgttcaact atcactatcg
    10261 cattagtgcc cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct ggtttgaatt
    10321 taccgaagcc tggcttcccg cgccgtcgca atgaatagct ggtccgggtt aatcgcaggc
    10381 gttaccataa cgcttttatt gttcaagcag gattatctat gaaacgtgct gtcgttgtgt
    10441 tcagtggagg ccaggattcc accacctgtc tggtgcaggc attacaacaa tatgatgaag
    10501 tccattgcgt gacgttcgat tacggtcagc gacatcgcgc agaaatcgac gtggcacgcg
    10561 aactggcgct gaaactggga gcacgcgcgc ataaggttct tgatgtaacg ctgctcaacg
    10621 agctggcggt cagcagcctg acacgtgaca gcattccggt gcctgattat gaacctgaag
    10681 ccgatggcat cccgaatacg tttgtcccag ggcgtaatat tttgttcctg acgctggcgg
    10741 caatatatgc gtatcaggta aaagcagaag ccgtaattac cggcgtctgt gaaacggatt
    10801 tctccggcta cccggattgc cgcgatgagt ttgtgaaagc actaaaccat gccgtcagtt
    10861 tgggcatggc gaaagatatt cgttttgaaa cgccgctgat gtggattgat aaagcggaaa
    10921 cctgggcgct ggcagattat tacggcaaac tggatttagt ccgtaacgaa acgttgacct
    10981 gctataacgg cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat ttacgcgcca
    11041 acggtttgaa tcattatctg gccgataaac cgacggtgat ggcagcgatg aagcagaaaa
    11101 ccgggttgag gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc ggcaccacaa
    11161 aacgtttact taaccatctg ctccagcttt tcgcgcaatt ccccttccag agctaatgct
    11221 ttctgcgttt taagatcaat acaaacaaac gtaataagcg catccgctac cacctgccct
    11281 tccggctcca gtgtaatgac ctggtttaag atgccgcttt taccgtttaa ttgctgcaac
    11341 tgactggtaa tagttaacag gtcgcttaat accgctggac gacgatagtt aatattgata
    11401 ttgaccacga cgaaggcgat gttatgggcc gtcatccact gaaaactgtc gctattttcc
    11461 accccatccc agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt gacgtgctgg
    11521 taaacgtcga gatgatatcc acgaactttg atttgtgttt gcatagcgca ataaccttac
    11581 gttgttgtta taagtacaga ggtcataact ggtatgacct cttcagtctg gcaaaaaatt
    11641 gccactatgc aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg
    11701 cccatccccg gcacctgctt tagatcctcc accgttttaa acggaccgta ctcttcgcga
    11761 taactgacaa tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct
    11821 tccgcgctgg cattattaat gctgacccgg gtgccttctt cgtcactggc tttcgccggt
    11881 actgctgctt tactttgtgc tgcaggagct tccgctttgg tttctaccgc cgtcggtttc
    11941 gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag ggacagtgta
    12001 atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa
    12061 tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg
    12121 cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg gcttcaggat
    12181 tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc
    12241 agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc
    12301 tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct
    12361 tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac
    12421 gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc
    12481 agaccggcag cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc
    12541 tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt
    12601 tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgac gcgcagcacg
    12661 aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccgacgc gccgttttca
    12721 cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc
    12781 aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc ttgctctgca
    12841 ccggccagag actcggtgtc gttgcttgcc acatcgctca ctttctgctg cagcgcatag
    12901 tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct
    12961 aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa
    13021 gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt cagttcgtcg
    13081 gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg
    13141 gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc
    13201 gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg
    13261 gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc
    13321 tgcatcgttg cggcaaccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa
    13381 ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg ctgcttcgcc
    13441 gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc
    13501 gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacgcc gttaatcagc
    13561 tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc
    13621 tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg
    13681 gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag ctcacgagcg
    13741 tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc
    13801 atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg ctgcatacga
    13861 ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg gtcattcact
    13921 tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac
    13981 gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag
    14041 ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat
    14101 cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacac
    14161 agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc
    14221 acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga
    14281 agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag
    14341 tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc
    14401 cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag
    14461 ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc
    14521 tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt
    14581 atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgctatc
    14641 taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta
    14701 ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca
    14761 ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat gtccagatcg
    14821 gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattagcact
    14881 gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc gatcggcagt
    14941 acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg
    15001 gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc
    15061 gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc agggttgatc
    15121 cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga ctcctgcatc
    15181 acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca tgcggtttca
    15241 atggtcagca agtcaccgcc cacttccgtc cacgccagac cgattacctg accgacacgg
    15301 ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta
    15361 tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg
    15421 cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa
    15481 cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta
    15541 cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta
    15601 taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag
    15661 ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg
    15721 ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc cggatcgcct
    15781 cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcggatt tttcacgccc
    15841 actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga
    15901 ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg
    15961 gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg ccctaccagg
    16021 cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg
    16081 cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc
    16141 aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca gtcgatataa
    16201 ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct
    16261 gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg
    16321 gcttcgtttt cgtccggcac gtcgtccatt tcaccgagtt ctttctgaat agctttcatt
    16381 tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga
    16441 atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc
    16501 agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc
    16561 atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt cagcgacgtc
    16621 agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca
    16681 gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc
    16741 ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc
    16801 tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg
    16861 gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg
    16921 accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc
    16981 cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat
    17041 acggggattt caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt
    17101 tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag
    17161 tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga
    17221 taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc
    17281 ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat caattaccga
    17341 ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag
    17401 cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt
    17461 cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa
    17521 cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc
    17581 ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga actcagggat
    17641 aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc
    17701 tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg
    17761 gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga
    17821 ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc
    17881 agctaccgta ccttcgatca gtttcaacag tgcctgctgt actccttcac cggaaacgtc
    17941 tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac
    18001 aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct tctgaatgat
    18061 gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt
    18121 gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac cggaaccggt
    18181 cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt
    18241 gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg cctgttcctg
    18301 gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg
    18361 ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac
    18421 acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct
    18481 tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat
    18541 gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag
    18601 gcgcaagccg cttacagcgt tgtgccgccc tagataagta tagcggcaca gttgcgcctc
    18661 tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg
    18721 gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta
    18781 tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg ggcatgaatt
    18841 tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa
    18901 ttcggcaggc aaaaacgttt accttttgcc cctgcggtca acaagaaagc gcccatcgag
    18961 gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag
    19021 atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata gatatctttt
    19081 tctgggtttt ccgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct
    19141 tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa
    19201 gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca
    19261 aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaaacc
    19321 tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt tatgggtacg
    19381 gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg aaagaggctt
    19441 ttcgcacggc tccatgcaaa attcatgaca aaaaaaagcc caccacctgg cggtgacggg
    19501 cctttgtgcg aatttagcgc gttatgctgc gtaaattacg cctgctggtt catcagctcg
    19561 ttgaaagtgg tttctttttc agtcactttc gctttcgcca gtacagcttc aacagcctgt
    19621 tcttccagag caacattgcg catgttgtcc atcagttctt tgtttttgct gtagaactcg
    19681 ataacttctt tcggatcttc gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc
    19741 tcttcgtcag ctttcagctc gttggtgcgg ataacttcgc ccagcagcag gccaacaact
    19801 acgcggcgtt tagcctgttc ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg
    19861 ttgccaccga aacgctgtgc agcctggcga cgcagaacgt cgatttcgct gtcgatcagc
    19921 gcagccggta cgtcgatgtc gttagctttt accagacctt cgatcgcctg agacttaacg
    19981 cggttacgga tggcgctctt cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga
    20041 ccttctacgg aaccatcttc aacgccgaaa cgtttgatga attctgcagt cagttccggc
    20101 agttcacgct cttcaacttt cttcaggttg atagcgaatt tcgctgcttt acctttcagg
    20161 ttttctgcgt ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc gccagctttg
    20221 tggcctttga taccgtcttc aaagcccggg atcatacgac cctggcccat cgccagtacg
    20281 aaatcagacg ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg
    20341 gttacgcggt cttctgcttc aacagcgccg tctttttctt tccaggtcgc ctgctgttta
    20401 cgcagagtat ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat cggtttttca
    20461 acttcgatcg cttccagacc ctgcagttca acttccggat aaacttcaaa ctctacagag
    20521 taagtgaagt cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga
    20581 ttgatttttt ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg
    20641 tcctggcgta cagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg
    20701 cggaagccgt caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc
    20761 tcgatgctgt cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca
    20821 actgaaactt gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc
    20881 aagcacaggt tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt
    20941 taccagaatc atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta
    21001 tgagtcgaga acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt
    21061 cgtctgaatg ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt
    21121 ttttcataaa atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta
    21181 cgcgatgctt cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc
    21241 ccactcctta atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc
    21301 cgctaaagta ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc
    21361 gctgaccagc acaactttaa aatggctttc agagccggct gggacattgt gacgatagct
    21421 ttcatccact acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg
    21481 ctcacgtatc atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa
    21541 atactagccg cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct
    21601 ggtttttccg tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat
    21661 cagcgtttct tttaccaggg gtgattattt cgtcaacatg atgaatttac atgccttacc
    21721 cacttcccct cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag
    21781 agtctctgag agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat
    21841 gcttgcagga tgcgcaaaac cgccaacaac tattgaagtt tccccgacga ttacactgcc
    21901 acagcaggat ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac
    21961 cgatcaggcg ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg
    22021 cgctctgcgt ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat
    22081 ggttggtccg aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgacgt
    22141 gtcccagggc aacgtgcgct acaacatcgc taccaaagcg gatatcgcca tcatcgctac
    22201 cgcgcagaat ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg ttgaaggtgc
    22261 gttccaggcc tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat
    22321 cgctgatatg tctcaggaca ccagcattca cgaattcatc aagcagaacg cgcgttaatt
    22381 tctgccctct ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac
    22441 gtatttttca acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac
    22501 cgctcgccct gacatccggc accttacagg cctggatgac ggtcgaaaat atcgatctca
    22561 aaaccattgg tttcttctct ctggtaggcc aggcttacgt ttttaaattc ctctggtcac
    22621 cgctgatgga ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca
    22681 cgcaaatcct gttattagtc gccattgcgg cgatgggttt tctcgaacca ggcacccaac
    22741 tccgctggat ggcggcgctg gcggtggtga tcgctttttg ctctgcctcc caggatattg
    22801 tcttcgatgc gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg ggcgcggcaa
    22861 tcagcgtgct gggttaccgt ttagggatgc tggtttccgg cggcctagcc ctgtggctgg
    22921 cagataaatg gttgggctgg cagggcatgt actggttgat ggcggcgctg ttgatcccct
    22981 gtattatcgc gacgttgctt gcaccagaac caaccgacac cattcctgtg cccaaaacgc
    23041 tggaacaagc ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta
    23101 ttttgcttct tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca
    23161 cgtttttgat tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggta aacaaaacgc
    23221 ttggcttact ggcgaccatt gttggcgcat tgtacggtgg gattttgatg cagcgcctgt
    23281 cgctgttccg ggcgctgctg attttcggca ttttacaagg tgcgtctaac gctggttact
    23341 ggctgctgtc gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa
    23401 acctctgtgg cgggatgggc acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata
    23461 agtcattttc cgctactcaa tttgccctgc tctcagcgct ttccgctgta gggcgagttt
    23521 atgtcggccc cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctat
    23581 tctccgtcgc cgctgccgta ccggggctta ttttgctgct ggtttgccgc cagacgcttg
    23641 aatatacacg agtaaatgac aactttatct cccgtaccga atatccggca ggttatgcct
    23701 ttgccatgtg gacactggcg gcgggcgtca gcctgttggc cgtgtggtta cttctgttga
    23761 cgatggacgc gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg
    23821 ttttagtcgc cctttctggc gtcgtgcttg gtggtttgct ggattatctg gcgctacgaa
    23881 aaacgcatct gacgtaatct gtaaatatta tttagctgta gtaatcactc gccgtaatta
    23941 ttacggctaa ataaacatca gcatcgctta ttaatatttg ttcttttgtg cggcttagcg
    24001 tttggaaatt attgacgcca tttataaatt ctatttttct tacacgattc agctaatgag
    24061 tctttatttt ctcaccaccc agttgtcact ttaatgataa ttatttgtta aataattgtt
    24121 ttatttcgca ttggttatac caattgccca ccaagactcc ttctgcactc cccttttgtt
    24181 a
//
LOCUS       DAZOYF010000050        23497 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZOYF010000050 DAZOYF010000000
VERSION     DAZOYF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.050"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..344)
                     /locus_tag="ACHUOF_001857"
     CDS             complement(<1..344)
                     /locus_tag="ACHUOF_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611052.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
     gene            694..1716
                     /locus_tag="ACHUOF_001858"
     CDS             694..1716
                     /locus_tag="ACHUOF_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFR0611053.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1716..2696
                     /locus_tag="ACHUOF_001859"
     CDS             1716..2696
                     /locus_tag="ACHUOF_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFR0611054.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2693..3451
                     /locus_tag="ACHUOF_001860"
     CDS             2693..3451
                     /locus_tag="ACHUOF_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0611055.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            3461..4270
                     /locus_tag="ACHUOF_001861"
     CDS             3461..4270
                     /locus_tag="ACHUOF_001861"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0611056.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            4270..5124
                     /gene="modD"
                     /locus_tag="ACHUOF_001862"
     CDS             4270..5124
                     /gene="modD"
                     /locus_tag="ACHUOF_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFR0611057.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            5150..7104
                     /locus_tag="ACHUOF_001863"
                     /pseudo
     CDS             5150..7104
                     /locus_tag="ACHUOF_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(7154..7408)
                     /locus_tag="ACHUOF_001864"
     CDS             complement(7154..7408)
                     /locus_tag="ACHUOF_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR0611058.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7609..8343
                     /locus_tag="ACHUOF_001865"
     CDS             7609..8343
                     /locus_tag="ACHUOF_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR0611059.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(8345..8956)
                     /gene="emtA"
                     /locus_tag="ACHUOF_001866"
     CDS             complement(8345..8956)
                     /gene="emtA"
                     /locus_tag="ACHUOF_001866"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR0611060.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            9056..9970
                     /gene="ldcA"
                     /locus_tag="ACHUOF_001867"
     CDS             9056..9970
                     /gene="ldcA"
                     /locus_tag="ACHUOF_001867"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR0611061.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            10065..11801
                     /locus_tag="ACHUOF_001868"
     CDS             10065..11801
                     /locus_tag="ACHUOF_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR0611062.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            12412..13826
                     /locus_tag="ACHUOF_001869"
                     /pseudo
     CDS             12412..13826
                     /locus_tag="ACHUOF_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13994..15064)
                     /gene="dadX"
                     /locus_tag="ACHUOF_001870"
     CDS             complement(13994..15064)
                     /gene="dadX"
                     /locus_tag="ACHUOF_001870"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR0611063.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(15074..16372)
                     /gene="dadA"
                     /locus_tag="ACHUOF_001871"
     CDS             complement(15074..16372)
                     /gene="dadA"
                     /locus_tag="ACHUOF_001871"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR0611064.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16702..18234
                     /locus_tag="ACHUOF_001872"
     CDS             16702..18234
                     /locus_tag="ACHUOF_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR0611065.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(18286..19005)
                     /gene="fadR"
                     /locus_tag="ACHUOF_001873"
     CDS             complement(18286..19005)
                     /gene="fadR"
                     /locus_tag="ACHUOF_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR0611066.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            19226..20767
                     /gene="nhaB"
                     /locus_tag="ACHUOF_001874"
     CDS             19226..20767
                     /gene="nhaB"
                     /locus_tag="ACHUOF_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR0611067.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            20913..21443
                     /gene="dsbB"
                     /locus_tag="ACHUOF_001875"
     CDS             20913..21443
                     /gene="dsbB"
                     /locus_tag="ACHUOF_001875"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR0611068.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(21489..22757)
                     /gene="umuC"
                     /locus_tag="ACHUOF_001876"
     CDS             complement(21489..22757)
                     /gene="umuC"
                     /locus_tag="ACHUOF_001876"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR0611069.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22757..23176)
                     /locus_tag="ACHUOF_001877"
     CDS             complement(22757..23176)
                     /locus_tag="ACHUOF_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR0611070.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         5685 a         6130 c         5812 g         5870 t
ORIGIN      
        1 tggagaatgt tatcattgct acaccctgtg cttaataact gcaactctcc gccgttcagc
       61 gatgctttga taattctgtt cccatagata tcgttatcaa aataaagcgt aaatatcata
      121 tttccgtaac tgttatcatc tctttttttg cacgtccgaa taaacgcatt cagtaaatta
      181 ctctctctaa gaaaataaat atcttcattg ttgttaatga cattaataac gtcgttctta
      241 aaatcctcaa agtcttgttg cggtaacttc tggttacgct gcttactatc agcaagcggt
      301 gaatccggac gacaacaaca taggttttgt ataaccccca gcattctttt cacctccgtt
      361 aacagtatat tcacgacact gaaacgagag aatgctgata attttctcgg cgagatatca
      421 aaaatcgctc attataaaac aagtaacttc aacgtcgcgt aaccgtggac gataaaaaaa
      481 caataatgtc attcatattt aaataattta gccttaaatg atttaaataa tattagccac
      541 ctgataaata catttttaat gatagagagg gtcaacaggc ttgcggaagt cgtcacatct
      601 tcatgttttc aatagctaaa ctttgatatt gaacatgatt tttatcgata tatatgaaag
      661 tatatatcga ttttactttt caggatgttc accatgccca taaccagacg gacgtttgcg
      721 caggcacttg catctaccct gctcctgcag tctctaccgt ccttttcaca aacagttaat
      781 cgttttgctt cgcaatctct tcccgaagcc caaaacatta cacgcatcgt cagcgcaggc
      841 gcgcccgccg atttgctctt gctggctgtc gcgccagaaa aaatggtcgg tttttcctct
      901 tttgattttg cccgtcaggc attaattcca ttgccagagc acattcgcca gttacccagg
      961 ctgggacgac tcgccgggcg cgccagcaca ctatcgctgg aagggcttat ggcgttacat
     1021 cccgatttgg tcgttgattg cggcaatacg gatgaaacct tgatctccca ggcacggcag
     1081 gttagcgaac agacacaaat cccctggtta ttgcttaacg gaaaactggc gcaatcagca
     1141 gaacagttaa caacgcttgg caaaacgtta ggcgaagagc accgcgccgc agaacaagcc
     1201 aatctcgcca gccacttcgt tggtgaagct caggcattcg ccacctcacc cgccgctaac
     1261 ctccgctttt atgctgcgcg cggtccccga gggctggaaa cgggcttaca gggttcgttg
     1321 cataccgagg cggcggaatt attaggtttg cacaacgtcg cgcaaatagc cgatcgccac
     1381 ggcctgacac aggtttccat ggaaaatctc ctgcgctggc agccggatat tattctggtt
     1441 caggaggccg ttactgcaga ttttattcgt cgtgatcctc tctggcaggg cgtgaaagcc
     1501 gttgcggaac aacgcatctt atttttaagt ggcttgccct ttggctggct cgatgccccg
     1561 ccgggaatca accgtcttct gggattacgc agacttcacg cctggctgga tcccgccatc
     1621 aatcgccagt ttaaaagtga catgcagcat tacgcccaac tgttctggca ttgttcactc
     1681 agtgatgccg actatcaaaa attggtggcg agctaatgag aatcgtaaac ggatgtattt
     1741 tgctgctggc agctatcagt atcacttttg ccgccgtaag tggggcttat catctcgata
     1801 tgcagcaact gctggcgcta attcttcgtc aagaaaatga gtctgtgcag gaacaaattg
     1861 tcttctggca aatccgcgtg ccgcgtattc ttgctgcgct gttactgggc gcagcgctgg
     1921 caggagccgg aaccacctat cagggaatgt tgcgtaatcc cttagtttca ccggatattt
     1981 taggcgtctc cgccggtgcc ggacttggtg cctgcgcggc aatcctgtgg ggattaccca
     2041 ttgtttacat ccagctgtac gcattttgcg gcgggctgat ggtggtcgcg ggcgtctggt
     2101 tgattacgcg tcgggtgacc cgccacgatc cgatcttaac cctggtgctg gtaggcattg
     2161 cgttaggaac gttgtgcgga gcggggatat cgctaattaa aacgctggcc gatccttaca
     2221 cccagttacc gtcaattacc ttctggttgc tggggggact gtccaccgtg accttacgcg
     2281 atctctgcta tgctgcaccg atcattctca taggctcctt gcccctgttt tttctccgct
     2341 ggcgcatgaa tttactcacg ctttccgacg acgaagcccg ttcgctggga ctgaacgtga
     2401 cgcgtctgcg ctttggattg attgtctgcg ccacactaat caccgccagc acggtagcaa
     2461 tagcgggcat aattggttgg atcggtctgg tcgttccgca cattgcccgc ttgttgaccg
     2521 gtcataacca tcagcagctg ttgcctatgg caatgtgtac tggcgctatt ctgctcctgc
     2581 ttaccgatac gctggcgcgc agcattggaa caacagaaat cccacttggc attctgaccg
     2641 cttttgttgg cgcaccgttt ttcctgcttt tgttgttacg cggaggtcgc cagtgacttt
     2701 gcttgccgtt cgtcatgcgt ctctgggtta ctcccgccat ccagtgctac gcgacgtgtc
     2761 cttcaccctg tcgcaaggaa caatttgttg tctgcttggc gcgaatggtt gcggcaaaac
     2821 cacactcatg cgctccattc ttggcgttat accgttatta aaaggcgaga tcctactgga
     2881 tagcctctct gtgcagacac tcagccatcg ccagcgcgca caggctattg cctgggtgcc
     2941 gcaggcgcac gacggtattt ttgctttcag tgtgctggat atggtgttga tgggcctggc
     3001 ccccaccata ggcgcttttt ccgttcccgg taagcaagaa cgcctgaaag ccacggagca
     3061 actggaaaag ctgggcattt tgcatctggc gacacgacgc tggaatacgc ttagcggcgg
     3121 tgaacgtcag ctggctttaa tcgcccgtgc actcgtccaa caaccgcgct tgttgttact
     3181 ggatgagccc gcctccagcc tcgatttcgg tcatcagatc caacttctgg acacgctggc
     3241 gcagctcaaa aataacggca tgacaatgtt aatgtcgact caccatacgc tccatgccaa
     3301 cgccattgcc gacagcatta tccaagtaga gccagatgga cgagtaacac aaggcttacc
     3361 gaccgaacag ttaacaacga acaaactggc ggccctgtat cgggtttctg ccgaccaaat
     3421 tcatcatcac ctctctgcaa ttagccgcta aggaacaatc atgctcattg atgatattga
     3481 ttttgcagac ctttatctgc agcaactcaa actggctcac cgcacagaaa aaacgcccga
     3541 tcactgggat caacgggcgg agaaaatggc ggaaaattgc gcctcgccaa cggacagcta
     3601 cctgcaacaa ctgatcgcta aaattgacct gcaaggcgct cagaccttat tcgatatggg
     3661 ctgcggacca ggcaccgtat cgctggcgct ggctgacaaa ctcactacgg tttatggcgt
     3721 tgattacagt caggggatgc tgaacgttgc cgcccgccgc gccgccgcac tgaaggctga
     3781 caatgtccac tggatccagc gcgcctggga agaggactgg agtgacctgc cacgctgcga
     3841 tattgccgtg gcttcccgct ccactctggt ggctgatatg cgccatgcaa tgagcaaact
     3901 gaataatcag gcacggcttc gcgtctatac aactcatctg gtcagcacct cgttcgtctc
     3961 tccagccatt cagcgggcgg ctggtcggga agtgatcgaa ttacccaact atatctttgc
     4021 gctcaacgtc ctgtatcaga tgggaattta tgcccacgtt gattttatcc gtggacaaaa
     4081 ttgccaacag gataacagca cctgggaacg ttttgagcag aacgtcagct ggagtcttgg
     4141 cgcgcttaac gacgatgagc gtgagcggtt atatcactgg tatcaacaac aagatgctcg
     4201 tgccctcgcc cctgccagcc gcgactgggc gctcatctgg tgggatagcg taccgcagga
     4261 ggcactgtga tgattttcct ttctcaggca caaatcgatg cgttactgct ggaagatatc
     4321 cagggaggtg acctgaccac gcgagcatta aatattggac accagcctgg ctatatagcg
     4381 ttttttcacc gtcagggcgg ctgcgtcagc ggcgtttctg tcgcgtgtaa gatgttaact
     4441 acgctgggat taactattga tgacgcggtc agcgacggtt cacaagcgaa cgcgggtcaa
     4501 cggctaatcc gtgcgcaggg taatgccgca gcacttcatc agggatggaa ggcagtccag
     4561 aatgtgctgg agtggagttg cggtgtttct gattatctcg ctcaaatgct ggcattgctt
     4621 cgtgaacgtt atcctgacgg caatatcgcc tgcacccgaa aagcaattcc gggcacccgc
     4681 ttactggctt cgcaggcaat tctggctgcc ggaggactaa ttcatcgtgc cggatgtgcg
     4741 gaaaccatat tattgtttgc caaccaccgc cattttcttc atgacaatca gggctggtca
     4801 ggcgcaatcg ctcagttacg tcgccacgca cccgagaaga aaattgttgt tgaagccgac
     4861 acgccgaaag aggctatcgc cgcgttacgc gcacaaccag acgtacttca gctcgacaaa
     4921 tttagtccgc aacaggcaac tgaaattgcg caaatagcgc cgtcgctggc tcctcactgc
     4981 acgctggctc tgaccggcgg aattaatctg acgacactca aaaattacct cgactgcggc
     5041 attcgccttt ttattacctc cgcgccttat tacgcggcac cagctgacat taaagtcagt
     5101 ctgcaaccca cagcctctat ttaattcttt gttaaacgga atttattcta tgagactcaa
     5161 aaaacgttat ctctgcacag cattatcact tgcctttacc cagcaggccg tagcggctca
     5221 ggagagcgac acgctgaccg tatggtccag tccggtatca tcgacgacga ccaccgttct
     5281 cgatcaaccc accatgaagg ccttggataa acagaatgtc gctcaggcat taagtgtcgt
     5341 ccccggcgtg gtgctgcaaa agtcaggcag ccgcaacgaa gaacaggtaa aagttcgtgg
     5401 ctttgatagt cggcaggtgc cggtctattt cgacggtgtg cccatttatg tcccctatga
     5461 cggcaacctc gatctggcgc ggattctgac caacaatctg ggggcagtcg aagtttccaa
     5521 agggtattcg tcgctgcttc atgggcctaa tcagatgggc ggagccatta atatcaccac
     5581 cctgaagaag caagtctggg atatcgccag ggatggagcc gtagtcagga caatgcctac
     5641 gatatgcatg cttcatttgc cgccagcagc gatcttgggt atttgcaagt cagcggtagc
     5701 cagctaaagc aggattttct cggcctgccg catggtgtaa ataatgatat tgcaggcaaa
     5761 cacggcaaga tgattaattc atcggctgat gataaacgcg gcattgtgaa gctcggtttt
     5821 acaccgcgcg aaaacgatga atacacgctg acttacatta agcaggatgg tgaaaaagat
     5881 aacccgccat acagcggaaa ttgtggtcaa aaatcacgct actggcagtg gccagagtat
     5941 gacaaagaaa gtttttatta tcagggagcg acccaactaa acgaccgttt taccctgaaa
     6001 agtcggctgt atcgcgacac ctttgaaaat acgctgatga tgtacaactc gctggctgat
     6061 ttgaaaaata aaaaaggcag ctacagccat tattccgatt acagcgacgg tgccggatta
     6121 caactggcag ccgatgtgcg tgaaaacgat ctgctgtcgt ttgccgttaa ctggaaagat
     6181 gacgtacacc gggaaaaagg tgcgccgcac gccgcttacg atcgctatga agatcgtacc
     6241 tggtcgctcg ccagtgaata tcaatgggct gctgctgata atgtcgatgt cgtggcggga
     6301 atcagctatg actggcgcga tagcgtagaa gcgaaaaaac acgagaaaga tggcagcatc
     6361 acccattatg acgacaacaa tcagtcagct tttaactggc aggtgatggg gaaataccac
     6421 tttgccaatg aagacactct ggcgctttcg tactatgacc gcacacgctt tccgacgctg
     6481 aaagaacgct ataccacatc caaacctgcg tataaccaga tagcgattgt taacccgcag
     6541 ctcaagccgg aacgcgctcg cggggtggat ttaacctgga atggtgcctt cacgcgcgac
     6601 tggggatttg aggtcagcgt ttactataac cgggtgagtg atgccatcct ctcgcacaat
     6661 atcaatgccg ataccattca aaatcagaac agcggcacgg tggattacag cggtctggat
     6721 gccggtatta aggggaaaat cagcaatata ctggatgtag gattgagcta cgctctgatc
     6781 cacgctgacg ccaaacgcaa agacatcggc aagataaccg atcttccaac gcagacaatg
     6841 accgcatgga tgacactcaa accatgggag ccgttaagcg taacgctgtc ggaagaggcg
     6901 cgttcctcca gctacagcaa cagtgacggt tcacaaaaag ccgccggttt tgcggtgacc
     6961 cacattcgag ccgattacac cttaggtcat ggcttcagcg tcaatgcgtc ggtcaataac
     7021 ctgtttgata cccaatatgc ctacagtgaa gggtttattg aagaaggtcg caatttctgg
     7081 gcaggtgtcg agtatacgtt ctgatgtgaa agcggatagc ggcatctgta tcgctatccg
     7141 ccttatgaaa atcttattct cttcgcagga ggcggaatac gcccagcacc aggatagcgc
     7201 ccaccaccgc caccaggaag ctgtgcaaat taaaaccgct gatggagccg tcaatgccaa
     7261 acatggtcgc cagccagccg ccgaccaccg caccgactat cccgagaata caggtcagga
     7321 aaaatccacc accatcacgc cccggcatga ttagcttgcc gataatgccg gctatcaggc
     7381 caaaaataat ccaggcaata attcccatgt tcaggccctc gtttcgatga atcggtcgca
     7441 agaaatgctt acgtcaacgc tctaagtata ggcaagggcg ggatcgatgc tgggcgtttg
     7501 tcggctgatt taagaacaac atgtaaaaaa tattcacgca tttgtattaa gttttgttaa
     7561 ctatgaccga taaaccaaag acagtttgtc agtcaggagt ttttccgcgt gagtcattac
     7621 catgagcagt tcctgaaaca aaatccgtta gccgtcctgg gcgtgttacg cgatttgcac
     7681 aaagccgcaa ttcctttgcg tctcagttgg aatggcgggc agctgatcag caaaatactg
     7741 gcaataaccc cggataaact ggtgctggat ttcggcagtc aagccgaaga caacatcgcc
     7801 gtgctaaagg cacagcacat taccattacc gccgaaactc agggtgcgaa agtcgagttt
     7861 actgttgaac aactacagca gagtgaatac ttgcagcttc cggcatttat taccgtaccg
     7921 cctcccacct tatggtttgt acaacgacgc cgatatttcc gcatctccgc cccactccat
     7981 ccgccttatt tttgccagac caaactggcg gataacagta cgttacgttt ccgcttgtat
     8041 gatttgtcgt taggcggcat gggcgcatta ctggaaacag caaagcctgc cgaattacaa
     8101 gaaggtatgc gcttcgctca aattgaagtc aacatggggc aatggggtgt ttttcacttt
     8161 gacgcccagt taatctccac cagcgagcgc aaagtgattg atggcaagaa tgaaaccatc
     8221 accactcccc gtctgagctt ccgttttctt aacgtcagcc cgacggtgga gcggcaatta
     8281 cagcggatta ttttctctct cgagcgagaa gcccgggaaa aagcggacaa agtgcgcgac
     8341 tgatttacat cgcgtccagt gcctgctcaa gtttgtagat atagcgcgga gcctgcggcg
     8401 caggatgatt tcgcgctacg tgatcgagga actcgtcagc atccaaatcg ttgattttgc
     8461 tgatcgcctt tttccgatct gacgagaaag tccgtagcag cgcacctgcc ccgttagcgt
     8521 atgacaccac cagcgcatat tgcagtacct tcggatcttc aatgcctgcc agcgggccgg
     8581 tttccagaat attcaggtaa gccgccccca ttgaaatatt acgctctgga tttttcagct
     8641 cgctggtcgt cggctcacca ctccagccca tacggcgata aacatcacgt ccggaggttg
     8701 aagcttttaa ctgcatcaaa ccaatggcat tcgatttact caccgcgttc ggattaccac
     8761 ccgattcgat agcgataatc gccgtgatca attgtggatc gacgccccag gctgcaccgg
     8821 ctttctggct tattggcatc cactgcatcg cacgttgcac cggaactttc gcgttccacg
     8881 gcgggttcgt atagtcatgc tttgatgaac aacccgctaa taacacaatc aaaaaggcaa
     8941 accatctcaa tttcacttct tctatcctta tagcctggaa tcgccgttga ggtgacaacg
     9001 gcacacctaa gcggcatgat atacatttcg tttctgttgt cagcaaggaa ttgccatgtc
     9061 tctgtttcac ttaattgccc catcgggtta cagcattaaa cagcacgccg cgctgcgtgg
     9121 tatccagcgc cttaccgacg cggggcatca agtcaataac gtcgaggtta ttgcccgtcg
     9181 ttgtgagcgt tttgcgggta cggaaacgga gcgtctggaa gatctcaatt cccttgccag
     9241 actgactacc cccaacacca tcgtgctggc tgtacgcggc ggttacggtg ccagtcgttt
     9301 actggcagat attgactggc aggcactggt ggctcgccaa caacatgacc cgttactcat
     9361 ttgcggacat agcgatttta ccgccattca gtgcggtctt ctggcgcaag gcaatgtcat
     9421 cacctttagc ggccccatgc tggtagcaaa ctttggtgcg gatgaactga acgcctttac
     9481 cgagcaccat ttctggctgg cgttacgcaa tgaaaccttc accattgaat ggcaagggga
     9541 aggcccgaca tgtcagaccg aaggcacgtt gtggggaggc aatcttgcga tgctgatttc
     9601 actgattggt acaccgtgga tgccaaaaat tgagaacggt attctggtgc tggaagatat
     9661 taacgtgcac cctttccgcg tcgaacgtat gctgttgcag ctctatcatg cgggaatttt
     9721 gccacgtcag aaggcgatta ttctcggtag ctttagcggc agcacgccca atgattatga
     9781 cgcaggttac aacctggagt cagtgtacgc gtttttgcgt tcccgcctgt cgattccact
     9841 cattaccggc ctcgattttg gtcatgaaca acgcacggtt acgctgccgt tgggcgcaca
     9901 tgccattctg aataacaccc gggaaggcgc tcagttgacg attagcggtc atcctgttct
     9961 taaaatgtaa aaaaatcacg cttcagggct aagtaaaacg ctgtctctgc cgctaatatg
    10021 gtagggttta tataagagac agcgtaatca ggagtaaccg accattggat gccacaacaa
    10081 taattagcct tttcatctta ggatccatcc ttgtcaccag cagtatatta cttagttcat
    10141 tttcttcccg tcttggcatt cctattctgg ttatttttct ggcgatcggc atgctggcgg
    10201 gagtcgatgg cgtcggcggt atcccgtttg ataattaccc cttcgcctac atggtaagta
    10261 acctggcgct ggcgattatt ttgctcgacg gcgggatgcg cactcaggcc agttcctttc
    10321 gtgtggcgtt aggaccggcg ctgtcgctgg cgacgctggg cgtacttatc acctctggtt
    10381 taaccggcat gatggcggcg tggctgttta atcttgattt gattgaaggc ttattaatcg
    10441 gcgctatcgt cggctccacc gatgctgcag cggtcttttc tctgctgggt ggtaaggggc
    10501 ttaacgaacg tgttggctcg acgctggaaa ttgaatccgg cagtaatgat ccaatggcgg
    10561 tctttctgac gattaccctg attgcaatga tccagcagca tgaaagcagt gtcagttgga
    10621 tgttcgtggt cgatattctg cagcaatttg gcctcgggat tgtcatcggt cttggcggtg
    10681 gttatttact gctgcaaatg attaatcgca tcgccctgcc tgccggatta tatccattgc
    10741 tggcattaag cggcggtatt ttaatttttg ctttaaccac ggcgctggaa ggcagcggta
    10801 ttctggctgt ttatctgtgc ggttttctgc tgggtaatcg gccgattcgc aaccgctacg
    10861 gcatcctgca aaatttcgac ggcctcgcct ggctggcgca aatcgccatg ttcctggtgc
    10921 tggggctatt ggttaaccca agcgatctgc tgcctattgc cattccggcg ctcattttgt
    10981 cggcatggat gatattcttc gcccgtcccc tttcggtatt tgctggattg ctccctttcc
    11041 gcggtttcaa tctgcgtgag cgcgtgttta tcagctgggt gggattacgc ggcgcggtgc
    11101 caatcatcct agccgtgttc ccgatgatgg cggggctgga gaatgcgcga ctgttcttta
    11161 atgtcgcctt ctttgtggtt ctggtttcac tgctcttgca gggaacatca ctctcgtggg
    11221 cggcgaaaaa agccaaagtg gtggttccgc ccgtcggacg tccggtgtca cgcgttggcc
    11281 tggatattca tccggaaaat ccgtgggagc agtttgttta tcaattgagt gctgataaat
    11341 ggtgtgtagg tgcggcactg cgcgatttgc atatgccaaa agagacgcgt attgcagccc
    11401 tgtttcgtga taaccagttg cttcatccta ccggcagcac ccgactgcgc gaaggcgatg
    11461 tattgtgtgt aattggtcgg gaacgcgatc tcccggcgct cggtaaactg ttcagccagt
    11521 cgccaccggt cgcgctggat caacgcttct ttggtgactt cattctcgaa gccagcgcca
    11581 aatatgctga tgttgcgctg atatatggcc tggaagacgg gcgagaatat cgtgataaac
    11641 agcaaacgct gggtgaaatt gtccagcagt tgctaggcgc ggcaccggtt gtcggtgacc
    11701 aggtagagtt tgccgggatg atctggacgg tggccgagaa agaagataat gaagtgttga
    11761 agattggcgt tcgggtggct gaagaggaag ctgaatctta aatttaatgc agcatctatc
    11821 gccggatgcg atgctttact gaacgcagcc tgagcctgta ataaacttgt catttcctga
    11881 ttttgatatg ctcatcccaa catcagaaga aggttaaatt atggatgaca aaccattgca
    11941 ggctctggct aatgaactca aaacaccggc tcaagaaagt cagcgtaagg gctttttccg
    12001 actgtaacag gtaataaaaa ccagccccgc attaccggtc aaccatcagg ggcccctgcc
    12061 tgacatcgcc tgaacatatt cccccacgca acctgccgca ctgacatagc ccacacaata
    12121 ggcgtttcat ccgcctgtag caccggatgc atgagcgatt tcttatagtt attcctttgc
    12181 ttaataacga ttatttccgg gtcgttcact ctcgtatttt ctttaatacg tcagagctac
    12241 ctaggccgta atacagcatg taccagaccc gacacggcat ttctgaccag gaaaaaagtc
    12301 aacgatgagt agtgatatca gcgcaaatga cgtcctgtgg ctcctttgcg gtgaccacat
    12361 tcacgtctcc ggcgctgcca gaggatattt atttcaagga aacgtagcat tatggctagc
    12421 tttaaagaca tctgcccatt cgcccccgcc attcccccat cttccgttaa cccaggcgta
    12481 cgacgcccgc cagggcggct cttctcaaga atcaaatttt gcagaacatg aagtatctca
    12541 gcccgcaaaa attcagctgg ggtgatgccc cctggcagat cattgacctc agtatcgcgg
    12601 gcaaagtgaa catacaggtg gacaacaata ccatcatcac gttggggact cgtctgaatc
    12661 agcaacataa cgagttcatg atggtcgcca aatggtgcga atgggctata cagcaggatg
    12721 gtttgcaaga aaacctgcaa aaaaacctgt acgaaatcct ggaagaaaac cagcaaaaca
    12781 aacagtcaga aattccccaa gaagacctga aagaaagtct ggaagaaatc aaggaaaata
    12841 tcctggaaga aaacctgcca gcaagccgga ttgaaaaccg ggcagaagca ttgcgcagaa
    12901 tgaaggaatg tctcattaca agacggagta tgctcaatct tagcaacctg ggattgactt
    12961 cactccctga gaatttgcct ccacatctga ttgaatttta ctgcagtaaa aacgtgttga
    13021 ccgcgttacc gaaggtgatg ccaaagtggc tgctggtgct tgactgtaca gataacgtct
    13081 tgatcttgtt accgaaggtg cagccctcaa aactgatggt actgaagtgc tatgaaaaca
    13141 gtattatctg gctgcctgag ctgtcgacta acctgagagt gattaactgt tctgaaaact
    13201 tcttgcaatt tttaccgccg tcgatgcccc agtacctgta taaactatcc tgtgccggca
    13261 acaatattaa ctcaatacct gatgagatgc tggagaactt gactcgcctg aaagtatttg
    13321 actgttctag taacgatttg atctcttcac cacggctgcc gcccaaacta ataatttact
    13381 actgtggaga aaacaagttt aaaacggtac aggtgccgca gccccagagc ctgaaggtgt
    13441 ttgactgtaa tggtaacccg tgggacaaag acaatttacc gacgctgctc aaagccgtcg
    13501 agggcctgaa aaaacagcag ggcctgaagg accttttgga ctttttgcac aaggaaggct
    13561 aggttgacct ggaaggactc gaggagctgg aggaccttat ggatctggag ttcctggatg
    13621 acccggaact cctggagcgc gtgaaggtac aggaggacct ggagctcctg gatcaacagt
    13681 tgggcctgtt gagtctggaa agacaacagg actcgcagcc tgttaatcaa caatctgaac
    13741 atgaacccga atctgcatca agggtgaagc gtgatttatc tgaagtcgac tccgagtcaa
    13801 caatgaagcg taagcgtttt atgtagaacg cctgtgcggc gagttcaggt tccggggaaa
    13861 tagtgtccat tcttttaatg gacactatca tgggcaatgg ctctgtcaca ttaacaacta
    13921 cagttgctga ccagccggat ggatgatttt ttcgcaacca gaagacgctg cctccgatcc
    13981 ggcttacaac aagttacact gtcacaaccg ggacgcgtaa cgccagtgcg cacatcaact
    14041 catagcccac cgttccggca gcggcggcga catcatcaat tttgatctcc ttgccccaca
    14101 gctcaaccgg cgtaccaatc cccgcctgcg ggcaaggcgt taaatcaacc gccagcatat
    14161 ccatcgagac ggtccccacc gtcatggtgc gcacgccgtc cactaaaaca ggagcaccgg
    14221 taggcgcatg gcgcggataa ccgtcggcgt atcctgcggc gacaatgccg attcgctgtt
    14281 catcgcgcgc agtatagcga ccgccgtagc ccacacgctc gcccgctttt agcgtctgga
    14341 caccaataat ctcactgctt agcgtcatca ccggacgtaa tccggtattg gcgatatcac
    14401 gccactgacc ggacggcgaa gcgccataca aaataatgcc aggccgaacc cagtcaaaat
    14461 gcgcttccgg gtgccacaga gtcgccgccg aattggacaa cgaacgccga cactccagcc
    14521 cctccgccgc ctgctcaata cgcgccatcg cgccggaaat tccatcagga tgttccgctt
    14581 cagcaaaatg cgacatcagg gtcatttcgc caacattcgc cattgcccgc aactgctgcc
    14641 agacggtaag cacgcgatcg gactggaagc ccaaccgatt catcccactg ttcactttaa
    14701 gataaatatc caacggtgct tttagccgcg cattttgcag tgctttgagc tgccagttgc
    14761 tgtgtacgca ggtggtcagg cggtgctggt cataaatctc cagatcctga gcatggaaaa
    14821 atccttccag catcaggatc ggccccttcc agccgcgctc acgtaacgtt attgcctctt
    14881 ccaggttaag taatgcaaag ccatcggtgg ccccgagcgc gctccagata cgctcaatac
    14941 catgcccgta agcgttcgct tttaccaccg accagacgcg cgcgtgcggc gcggcctggc
    15001 ggacaatgga cagattctgt tttaatgcct gcagatcgag gctggcctgt atcggacggg
    15061 tcatctcgtt tccttagctg tgtgcgccat gtaaatggcc cggacgtgat ggcgtaaatc
    15121 cacggctgta gcgcgctacg cttagatcct catatgggat cgctggcgtg cgaccagaga
    15181 gcagatcgct taacaactga ccggaaccgc aagccatcgt ccagccgagc gtgccgtgac
    15241 cggtattcag ccacagattt ttaaagcgtg tactcccgac aaccggcgta ccgtctggcg
    15301 tcatcgggcg caaacccgtc cagaaagtcg cctgctcgac atgaccgccg cgtggataga
    15361 gatcgcgaac caccatttcc agcgtttcac gacgcggttg caacagctcg gtgttaaaac
    15421 caacaatttc cgccattccg ccaacacgaa tgcggttatc gaaacgggta atggcgattt
    15481 tgtaggtttc atcaagaatg gtggatactg gcgcaccatc ttcctgcgca attggaatag
    15541 tcagcgagta gcctttcagc ggatacaccg gaatatcaac aatgcctttg agcatcgccg
    15601 tcgagtaaga accaaacgcc atcacatacg catcggcctt aatcacttca tcgccaaact
    15661 tcacgccgta gatttgctcg ccgtcgcaaa gcagttggtc aacgggcgta ttaaagcgga
    15721 atttaacccc cgcctgctct gccatccgcg ccagattctg ggtaaacagc tggcagtctc
    15781 cggtttcatc attgggtaac tgcaggccgc ccgtcagttt gtgcgctact tctgccagcg
    15841 cgggatccac ttccgccagg cggctggatt ccagcagctg atacggtacg ccggcatctt
    15901 ccagcacggc gatatcgcgg gtcgcatttt catactgttg ttcggtacgg aacagttgca
    15961 gcgtcccacc ctgacgccct tcatactgaa tattggtttc ggcgcgcaat gctttcaggc
    16021 aatcacggct gtattccgcc agacgcacca tccgcccttt gttttccatg tagtggctgg
    16081 tgtcgcagtt acgtaacatt tgccacatcc atttcaactg aaactgcgta ccgtcgagac
    16141 gaaccgccag cggcgcatgg cgctggaaca tccatttaat cgcttttaaa ggcacacctg
    16201 gtgctgccca cggtgcagca tatccggggg agatttgccc ggcattcgcg gcactggttt
    16261 ccagggcggc ccccggctcc cgatcaatga cggtgacctc atgtcctgcc tgatttaagt
    16321 accaggcgct ggcaacgcct accacaccac ttcccagtat gacaactcgc atagccactc
    16381 cgataacggt agatacagta aaagaataat aatctaatta catcttgata actcagttga
    16441 aaatattatt caacatacgg cttttttatg gtgagctgac tcacatctcc ctgactctgc
    16501 ggcaatatgg cgtctttagt atctcctgct gaacgccctt tttatccagc ataaaattcc
    16561 gttcagaagc ggattagtgg cactctgata ataagaaatc gcaaagaaaa aaattctccg
    16621 gcgacgcggt ttttaacagg tgttctatgc ttgaaatgag gtgctccaca cagagagcgc
    16681 cgacaacaat gaggtgcgcg tatggcgacg atcgattcta tgaataagga caccacacgt
    16741 ttgagcgatg gacccgactg gacgttcgac ctgctggatg tttatctggc agagatagac
    16801 cgggtggcga aactctaccg gctggatacc tacccgcacc agattgaagt gataacctca
    16861 gaacagatga tggatgccta ttccagcgtc ggcatgccaa ttaactatcc gcactggtca
    16921 ttcggtaaaa agtttatcga gactgaacgg ctgtataagc acggtcagca agggctggcc
    16981 tatgaaatcg tcattaactc taacccgtgt atcgcttact tgatggaaga gaacaccatt
    17041 actatgcagg cgctggtgat ggcacacgcc tgctatggac ataactcttt ctttaaaaac
    17101 aattgcttat tccgcagttg gaccgacgcc agttcgattg tcgattatct gatttttgcc
    17161 cgtaaatata ttaccgagtg cgaagaacgt tatggcgttg atgaagtaga acggcttctg
    17221 gactcgtgcc acgcgctgat gaactacggc gtggaccggt acaaacgccc gcaaaaaatc
    17281 tcgctgcaag aagagaaagc ccggcagaaa agtcgcgaag agtatctaca aagtcaggtc
    17341 aatatgctct ggcgtaccct gccgaagcgc gaggaagaga aaacggttgc tgaagcgcgc
    17401 cgctatccgt ccgaaccaca agaaaacctg ctctatttta tggagaaaaa tgcgccactg
    17461 ctggaatcat ggcagcgtga aatcctgcgt attgtgcgta aggtgagcca gtatttttat
    17521 ccgcaaaaac agactcaggt gatgaacgaa ggctgtgcga ccttctggca ctataccatc
    17581 cttaaccatc tgtatgatga agggaaagta acggaacgtt ttatgctgga gtttttgcac
    17641 agccacacca atgtggtctt ccagcccccc tataacagcc cgtggtacag cggcatcaac
    17701 ccgtatgccc tcgggttcgc catgttccag gatattaaac ggatttgtca gtcgccaacg
    17761 gaagaagaca aatactggtt cccggatatc gccggttccg actggctgga aacgctgcat
    17821 ttcgcgatgc gtgatttcaa agatgagagt tttatcagcc agtttctgtc accgaaagtg
    17881 atgcgtgatt tccgcttctt caccgtgctg gatgatgatc ggcataatta tctggagatt
    17941 tccgctattc ataatgaaga aggttatcgg gagatccgta accggttatc gtcgcaatat
    18001 aacttaagta atctggagcc gaatattcag atctggaacg tggatttgcg cggcgaccgt
    18061 tcgctgacgc tgcgttatat tccacataat cgcgcaccgc tggatcgggg gcgcaaagaa
    18121 gtcctgaagc atgtgcatcg cctgtgggga tttgatgtga tgctcgaaca gcaaaacgaa
    18181 gacggcagca tcgagttgct ggaacgttgc ccgccaagaa tgggaaatct gtaaaaacaa
    18241 caaaaaaccc ctcgtttgag ggggttgctc tttaaacgga agggattatc gcccctgaat
    18301 ggctaaatca cccggcagat ttttctgcat ccggtgccaa atctcgccac tctcatgccc
    18361 atagcgacgc actgtttcgt acacctgatc gtgcgcgcct tcactgcaca acgccgacag
    18421 tttgtggtag aagcccagcg ccagactgcg cgcttccgga ttggcgaaat agtgacgacc
    18481 aatgcgcgta tacagccctt tcatcccgtt aaggatcaga ccgtaaatcg ggttgccgga
    18541 agcaaacgcc aggccgcgga atatgttgta atccagctcg gcaaaggcat cggcgtgatc
    18601 ggccacttca ttagcggtag ccagcacttc ctgcgcttta tcgggatgct gacgaaacgc
    18661 ggtgcgaata aaaatagtgg aaatattggt acgcaccgac agcaaattat caataagctg
    18721 cggcacactt tcgtgatcca gtcgcgccag tgtttcaagg atatttaaac cggaagtttc
    18781 ccagaaatta ttcaccttcg tcggcttgcc atgttgaatg gtcaaccagc catctcgtgc
    18841 cagacgctgt aacacttcac gtaacgtagt acgcgttacg ccaattaatt ctgaaagttc
    18901 acgttctgcg ggcaaaatag tcccgggagg gaagcggtta ttccagatac tttcaataat
    18961 gtactcttcc gcgaaacccg ccgggctttg cgccttaatg accatagtga gatttccata
    19021 acacagcaaa acaaagttgg actcatcata ccagagggct aactacgctg atagcagaca
    19081 aaaagaagaa aaagggatca aggcttcatt ttccgcatct ctttcccctt gactatcaag
    19141 acttgcctca ctcctgcctg accgttaagc tttgcgttta aacataaaaa catcattgat
    19201 tatttgtaag gtaagggaaa ccatcatgga gatctcctgg ggccgcgcgc tatggcgcaa
    19261 ttttttgggc cagtcccccg actggtacaa actcgccctc atcattttct taatcgtaaa
    19321 cccgttaatt ttcctcatca gccctttcgt cgcgggctgg ttgctggtag cggaatttat
    19381 tttcactctg gcgatggccc tgaaatgcta cccgctactc cccggtggtc tgttggctat
    19441 cgaagcggta ttcatcggca tgaccagcgc ggaacacgtc cgtgaagagg tggcggcaaa
    19501 tcttgaagtc ttgctgttat tgatgtttat ggtggcgggt atctatttta tgaaacagct
    19561 gttgctgttc atatttaccc gtttgctgtt aagcattcgc tccaaaatgc tgctgtcgct
    19621 ctctttttgc gtggcggcag cgttcctctc cgcgttcctc gatgccttaa ccgtcgtggc
    19681 ggtggtgatc agcgttgcag tcggttttta tggtatttat catcgcgtag cctcttcccg
    19741 taccgaagac accgacctgc aagacgatag tcatatcgac aagcattaca acgtggttct
    19801 ggaacagttc cgtggctttc tgcgtagcct gatgatgcat gccggtgtcg gcaccgcatt
    19861 aggcggcgta atgaccatgg tgggcgaacc acagaacctg atcatcgcta aagcggctgg
    19921 ctggcatttt ggcgatttct tcctgcgcat gtcgccggtg accgttccgg ttctgatttg
    19981 tggcctgtta acctgcctgc tggtagagaa gctgcgttgg tttggctacg gtgaaacgct
    20041 gccggagaaa gtccgcgaag tgttgcaaca gtttgacgat caaagccgcc accagcgtac
    20101 ccgtcaggat aaaatccgtc tgattgtcca ggcgattatt ggcgtctggc tggtgactgc
    20161 gctggcgttg catctggcgg aagttggctt gattggtttg tcagtcatta ttctggcaac
    20221 atcattgacc ggtgtcaccg atgaacatgc tatcggtaaa gccttcaccg aatctctgcc
    20281 attcaccgca ctgttgacgg tgtttttctc ggtagtcgcg gtgattatcg accaacaact
    20341 gttttcgccg attattcagt ttgtgttgca ggcatctgaa catgctcagc tgtcgctgtt
    20401 ctatattttc aacggtctgc tgtcatccat ttcggataac gtcttcgtgg ggacgattta
    20461 tatcaacgaa gcgaaagcgg caatggaaag tggcgctatc acgttgaagc aatacgagct
    20521 gctggcggtc gccattaata ccggtaccaa tctgccctct gtcgctacgc cgaacggtca
    20581 ggctgcgttc ctgttcctgc tgacctctgc actcgcgcca ttgattcgcc tctcttatgg
    20641 ccgcatggtg tggatggccc tgccttacac cctcgtactg acactcgtcg gcttgctctg
    20701 cgtcgagttt acgcttgccc ctgtaaccga atggtttatg caaatgggct ggatagcaac
    20761 gctttgataa caacttaccg ggcatataaa tgcccggttt gccttttcgc ccgataattg
    20821 tccaattgcg tcctattttt gctgcctcct ggtggcgcag cgaatgaatt ggtttaaact
    20881 gcgcactcta tgcatattgc agggaaatga ttatgttgcg atttttgaac caatgttcac
    20941 aaggccgggg cgcgtggctg ttgatggcgt ttactgctct ggcactggaa ctgacggcgc
    21001 tgtggttcca gcatgtgatg ttactgaaac cttgcgtgct ctgtatttat gaacgctgcg
    21061 cgttattcgg cgttctgggt gctgcgctga ttggcgcgat cgccccgaaa actccgctgc
    21121 gttatgtagc gatggttatc tggttgtata gtgcgttccg cggtgtgcag ttaacttacg
    21181 agcacaccat gcttcagctc tatccttcgc catttgccac ctgtgatttt atggttcgtt
    21241 tcccggaatg gctgccgctg gataaatggg tgccgcaagt gtttgtcgcc tctggcgatt
    21301 gcgccgagcg tcagtgggat tttttaggta tggaaatgcc gcagtggctg ctcggtattt
    21361 ttatcgctta cctgattgtc gcggtgctgg tggtgatttc ccagccgttt aaagcgaaaa
    21421 aacgtgatct gttcggtcgc taatccattc ggcgctcctg cgggagcgct tttttcctgc
    21481 cgctatattt atttgacccg cagtaaatca gaacttcgcg ttgtataacg tggtgaaagc
    21541 atagctcgct tcatctgcca ttgttgttgg atcccctgcc cggcaaaata gagtgttcct
    21601 ctgccctctt tagcattcag agtatccatt accgccatca attgctcact cccggggcgc
    21661 ggtgcgttgt catcgaataa attgagctgc gcgactccct gactgaagaa atcccccagc
    21721 atcacgcccg ctttttggta gcgatggccc gcttgccaga tggcatccag agatcgcgta
    21781 gcagcgttaa tgatatccct gctgtcctga gtgggcgtca gcagttttac cgacgcgcta
    21841 ttgccgtaat aaggttcatt gagcgcaaat ggtgacgtct taataaatgt ggagataaac
    21901 cgacaatatt gatgctcact gcgaagtttt tccgccgccc gggcagcgta actacaaatg
    21961 gcctgccgca tcgacgtata atccgtgatg cgttcaccaa acgagcggga acagataatt
    22021 tcctgcttcg tcggtgcaaa ctcttccagt tgcaaacagg gttcgccgcg cagttcacgc
    22081 accgttcttt cgagcatgac attaaaatgt ttacggataa accggatatc tgtatccgcc
    22141 aaatcgagaa cggttttgat ccccatcgcg tccagttttt tgctgatccg ccgtccaatc
    22201 ccccagacgt catccacggg gagagcagac attaatttac gctggcgttc cagatttgat
    22261 aaatccacca ccccacccgt ctgccgctgc catttttttg ccgcatgatt ggcaagctta
    22321 gccaacgttt tggtctgggc gatccccaca ccaacagtaa gatgggtacg ttgtagcacc
    22381 gttgcgcgaa tttctctgcc aaaatcagtc agatcgcgac aattacgcac acctgtcaga
    22441 tcgcagaatg cttcatcaat actgtaaatc tcgacgcggg gcgatagttc ttccagcgtc
    22501 gacatcaccc gattgctcat gtctgcgtaa agctcataat tgctgctaaa gcaaaccaca
    22561 ccacagcgac gaaacagatc tttttgtttg aaccagggat cgcccatttt aacgcccagc
    22621 gcctttgcct cagcgtttcg ggcgataacg caaccgtcat tattcgatag cacaaccacc
    22681 ggtttacccc ataaatcagg gcgaaacacc gtctcacagc tggcataaaa cgcgtttaca
    22741 tcacagaggg caaacatcag cgcatcgcct taacgacgtg gatcaccaca ccaaagacat
    22801 ccagcgtatc ttcgctactg atggtaatgg gcgagtacgc actgttcatg ggaataagct
    22861 gtaccgtcgg gcgtagttgc aattttttca ccgtaaactc gccgtcaaca gcagcgatga
    22921 caatatcacc atggctggcg gtaatagcac tatcgacaat cagtaaatca ccgtcactaa
    22981 ttccaccatc aatcatagaa tcaccacttg ctttgacgaa gtaagtcgcg ctgggatgct
    23041 ggatcaacag ttgattcaga tcgatgcgct gttcaacgta atctgctgcc ggtgaaggaa
    23101 agccacactg aacaagatcg ctaaatagcg gaaaagtcac aatttcgcgg agatccgcag
    23161 gcttgataaa caacataata atctgcctga agttatactg tttttatata cagtggtctg
    23221 ttcttgccag cagatcaata ctgattcagg ctatcaatat ttgtcgctgc atagactgct
    23281 gatttttcgt tctcttatcc tgtgctcacg tggccttctg gcgacgacgc tcatccagca
    23341 gaaatgaaaa atatcacccg gcaaaaaaat agaatagaag cattcgccat aatgacatta
    23401 aacattgttt gatatttatc atattaatag aaataaagac attgacgcat cccgcccggc
    23461 taactatgaa ttagatgaag taaaatttat taatagt
//
LOCUS       DAZOYF010000051        23283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZOYF010000051 DAZOYF010000000
VERSION     DAZOYF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.051"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..246
                     /locus_tag="ACHUOF_001878"
     CDS             <1..246
                     /locus_tag="ACHUOF_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0611071.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHUOF_001879"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHUOF_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR0611072.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACHUOF_001880"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACHUOF_001880"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0611073.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="ACHUOF_001881"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="ACHUOF_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFR0611074.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACHUOF_001882"
     CDS             complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACHUOF_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR0611075.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACHUOF_001883"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACHUOF_001883"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR0611076.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="ACHUOF_001884"
     CDS             complement(4442..5650)
                     /locus_tag="ACHUOF_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0611077.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="ACHUOF_001885"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="ACHUOF_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR0611078.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="ACHUOF_001886"
     CDS             complement(6445..8130)
                     /locus_tag="ACHUOF_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR0611079.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="ACHUOF_001887"
     CDS             8400..8777
                     /locus_tag="ACHUOF_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR0611080.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACHUOF_001888"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACHUOF_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR0611081.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="ACHUOF_001889"
     CDS             9224..9511
                     /locus_tag="ACHUOF_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR0611082.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="ACHUOF_001890"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="ACHUOF_001890"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR0611083.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="ACHUOF_001891"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="ACHUOF_001891"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR0611084.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="ACHUOF_001892"
     CDS             11268..11744
                     /locus_tag="ACHUOF_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR0611085.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="ACHUOF_001893"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="ACHUOF_001893"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR0611086.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14502
                     /gene="potG"
                     /locus_tag="ACHUOF_001894"
     CDS             13303..14502
                     /gene="potG"
                     /locus_tag="ACHUOF_001894"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR0611087.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLGASGCGKSTLLGASGCGKSTLLRMLAGFEQPSAGQIMLDG
                     VDLSQVPPYLRPINMMFQSYALFPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVH
                     MQEFAKRKPHQLSGGQRQRVALARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDIL
                     ERVGVTCVMVTHDQEEAMTMAGRIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVN
                     VFEGVLKERQEDGLVLDSPGLVHPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANG
                     CNFAVGEVIHIAYLGDLSVYHVRLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVD
                     SCVVLTV"
     gene            14512..15465
                     /gene="potH"
                     /locus_tag="ACHUOF_001895"
     CDS             14512..15465
                     /gene="potH"
                     /locus_tag="ACHUOF_001895"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR0611088.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15462..16307
                     /gene="potI"
                     /locus_tag="ACHUOF_001896"
     CDS             15462..16307
                     /gene="potI"
                     /locus_tag="ACHUOF_001896"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR0611089.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16367..16855
                     /locus_tag="ACHUOF_001897"
     CDS             16367..16855
                     /locus_tag="ACHUOF_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR0611090.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16896..18023
                     /gene="rlmC"
                     /locus_tag="ACHUOF_001898"
     CDS             16896..18023
                     /gene="rlmC"
                     /locus_tag="ACHUOF_001898"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR0611091.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18198..18929)
                     /gene="artJ"
                     /locus_tag="ACHUOF_001899"
     CDS             complement(18198..18929)
                     /gene="artJ"
                     /locus_tag="ACHUOF_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR0611092.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19221..19889)
                     /gene="artM"
                     /locus_tag="ACHUOF_001900"
     CDS             complement(19221..19889)
                     /gene="artM"
                     /locus_tag="ACHUOF_001900"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR0611093.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPESQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19889..20605)
                     /gene="artQ"
                     /locus_tag="ACHUOF_001901"
     CDS             complement(19889..20605)
                     /gene="artQ"
                     /locus_tag="ACHUOF_001901"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR0611094.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20612..21343)
                     /gene="artI"
                     /locus_tag="ACHUOF_001902"
     CDS             complement(20612..21343)
                     /gene="artI"
                     /locus_tag="ACHUOF_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR0611095.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21361..22089)
                     /gene="artP"
                     /locus_tag="ACHUOF_001903"
     CDS             complement(21361..22089)
                     /gene="artP"
                     /locus_tag="ACHUOF_001903"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR0611096.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22307..22822)
                     /locus_tag="ACHUOF_001904"
     CDS             complement(22307..22822)
                     /locus_tag="ACHUOF_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0611097.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         5705 a         5758 c         6026 g         5794 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccctagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atgctaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc gccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccgtgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctggg cgcatccggc
    13501 tgtggcaagt ccacgctgct gggcgcatcc ggctgtggca agtccacgct gctgcgtatg
    13561 ctggcaggtt tcgaacaacc ttctgccgga cagataatgc ttgatggcgt cgatttgtca
    13621 caggttccgc cttacctgcg ccccatcaat atgatgtttc agtcttacgc gctgtttccg
    13681 catatgaccg tggaacagaa catcgctttt ggcctgaaac aggacaaact accgaaagcg
    13741 gaaatttcca gtcgggtcaa tgagatgctc ggcctggtgc acatgcagga gttcgccaaa
    13801 cgtaaaccgc atcagctttc tggtggtcag cgacagcgtg tggccctggc ccgaagcctt
    13861 gccaagcgcc cgaaactatt actgctcgac gagccgatgg gcgcgctgga taaaaagctg
    13921 cgtgaccgga tgcagcttga agtggtggat attctggagc gcgtcggtgt gacttgtgtg
    13981 atggtcaccc acgatcagga agaggcgatg accatggcgg ggcgcatcgc cattatgaat
    14041 cgtgggaaat ttgtccagat tggcgaaccg gaagagatct acgagcatcc gactacccgc
    14101 tatagcgccg aatttattgg ctcggtaaac gtctttgaag gcgtactcaa agagcgtcag
    14161 gaagatggct tggtgcttga ttcgccgggg ctggtgcatc cactgaaagt cgacgcggat
    14221 gcctcggtgg tcgataacgt gccggtacat gtggcgctgc gcccggaaaa aatcatgctt
    14281 tgcgaagagc cgcccgccaa tggttgtaac ttcgcggtgg gggaggtgat acacattgcc
    14341 tatctcggcg atctttcggt gtatcacgtt cgtctgaaaa gtgggcagat gattagtgcc
    14401 cagctacaaa acgcccatcg tcatcgtaaa gggttaccga cctggggcga cgaagtgcgt
    14461 ttgtgctggg aagtggacag ctgtgtggtg ctgacggttt aaggagcaaa gatgaataca
    14521 cttgaacctg ctgcccagtc gaaaccgccg ggcggattta agctgtggtt gtcgcagctg
    14581 caaatgaagc atgggcgcaa actggtcatt gcgttgccat atatctggtt gatcttgctg
    14641 tttctgctgc catttctgat tgtctttaaa ataagcctgg cggagatggc gcgcgctatt
    14701 ccaccttata ccgaactgat ggagtgggct gacgggcaac tgtccatcac tcttaatctc
    14761 ggtaattttc tgcaactgac cgacgatccg ctctatttcg atgcttatct ccagtcgcta
    14821 caggtggctg cgatttcgac attttgctgt ttactgatcg gctatccgct ggcgtgggcg
    14881 gtggcgcaca gtaagccttc gacccgtaat attttattac tactggtgat cctgccgtcg
    14941 tggacctcgt ttctgatccg cgtttatgct tggatgggaa tattaaaaaa caacggtgtg
    15001 ctgaataatt ttctgctgtg gctgggggtt atcgatcaac cgctgaccat tcttcatacc
    15061 aatctggcag tttatattgg cattgtttac gcctacgtgc cgtttatggt gctgccgatt
    15121 tataccgcgt tgattcgtat tgattattcg ctggtggaag cagcgctgga tctcggtgca
    15181 cgaccgctga aaacgttctt tactgtgatc gtgccgctga ctaaaggtgg gattattgcc
    15241 ggatcgatgc tggtgtttat cccggctgtg ggcgagtttg tgatcccgga actgctcggt
    15301 ggcccggaca gcatcatgat cgggcgcgtg ctatggcaag agtttttcaa taaccgcgac
    15361 tggccggtgg cctcggcggt agcgatcatc atgttgctgc tgctaattgt gccgataatg
    15421 tggttccaca aacaccagca aaaaagcgtg ggagaacacg gatgaataat ttaccggtag
    15481 ttcgttcgcc ctggcggatt gtgattttgc tgctgggctt tacctttctc tacgcgccaa
    15541 tgctgatgct ggttatctat tcgtttaaca gctcgaagct ggtgacggtg tggggcggct
    15601 ggtcaacgcg ctggtatggt gagttattgc gcgatgacgc gatgatgagt gcggttggtt
    15661 caagcctgac aattgctgcc tgtgcggcaa cggcggcggc gatcctcggg actattgcgg
    15721 cggtggtgct ggtgcgcttt ggcaggtttc gcggatcaaa tggctttgcc tttatgatca
    15781 ccgcgccgct agtaatgcca gatgtcatca cgggcttgtc gctgttgtta ttattcgtcg
    15841 cgcttgctca tgccattggc tggcctgcgg accgcggtat gcttaccatc tggctggcgc
    15901 atgtcacttt ctgtacggct tatgtggcgg tcgttatttc gtcgcgtctg cgggaactgg
    15961 atcgctcgat agaagaagcc gcgatggatc tcggtgcgac gccgctgaaa gtgtttttcg
    16021 tcattacgct accgatgatc atgcccgcga tcatttctgg ctggttactg gcttttactt
    16081 tgtcgcttga tgatctggtg atcgccagct ttgtttccgg gccgggagcc accacgttac
    16141 cgatgctggt cttttccagc gtgcggatgg gggtgaatcc ggaaatcaac gccctggcaa
    16201 cattgatcct cggcgcggtc ggaattgtcg gatttatcgc ctggtatctg atggcgcgcg
    16261 cagaaaaaca gcggatacgc gacatccaac gtgcaagacg tggctgaaga cactaaaatt
    16321 tgccaacctg gctacataat gccgcgcatg tcgcggcatt gttttcatgg aagacgaaac
    16381 gttgggattt tttaagaaaa catcttcatc tcatgctcgc ctgaatgtgc ctgcgctggt
    16441 gcaggtggcg gcgctcgcca ttattatgat ccgtggcctc gacgtgctga tgattttcaa
    16501 tacgctgggc gtgcgcggta ttggcgagtt cattcatcgc agcgtacaaa cctggagttt
    16561 aacgctggtc tttttaagca gtctggtgct ggttttcatt gagatctggt gtgcgttttc
    16621 actggtgaaa gggcgtcgct gggcgcgctg gctatatctg ctgacacaaa tcaccgccgc
    16681 aagttacttg tgggcggctt cgctggggta cggttatccg gagctgttca gcattcccgg
    16741 tgaatcaaaa cgtgaaatct tccatagcct gatgctgcag aagctgccgg atatgctcat
    16801 cctgatgctg ctgttcgttc cctcgaccag tcggcggttc ttccagttgc aataatgtgt
    16861 ataatcgtcg cccctgatga tgtgaaggtc aatgtatgca gtgcgcactt tacgacgcgg
    16921 gtcgctgtcg ttcctgtcag tggataacgc agccgattcc agagcaactc tccgctaaaa
    16981 ccgccgatct taaaaatctg ctcgccgatt ttccggttga ggaatggtgc gcgccggtgt
    17041 caggcccgga acaagggttt cgtaataaag ccaaaatggt ggtgagtggt agcgttgaaa
    17101 aaacactgct cggtatgctg catcgcgatg gcacgccgga agacctttgt gactgcccgc
    17161 tttatccagc ctcatttgcg cccgtttttg cggcgctaaa accgtttatc gcccgagcgg
    17221 ggttaacgcc ctacaacgtg gcgcgtaagc gtggcgaact gaaatacatt ctgctgactg
    17281 aaagccagag tgatggcggc atgatgctgc gctttgtact gcgttctgat accaagctgg
    17341 cgcaactgcg taaggcgctg ccgtggttac acgaacaact accgcagctg aaagttatta
    17401 ccgtcaatat tcagccggta catatggcga ttatggaagg ggagacggag atctacctga
    17461 ccgaacaaca ggcactggcg gagcgtttta atgacgtacc gctgtggatc cgtccgcaaa
    17521 gtttcttcca gactaatccg gcggtcgcca gccagttgta cgccaccgcg cgcgactggg
    17581 tacgacagct gccggttaaa catatgtggg atctgttctg cggtgtgggg ggctttggtt
    17641 tacactgcgc gacgcctgac atgcagttaa ccgggatcga aattgcgcca gaggccattg
    17701 cctgtgcaaa gcagtcagcc gctgaactgg gcttaactcg tttacaattt caggcgctgg
    17761 actccactca gtttgccact gctcaggggg aggtgccgga gctggtgctg gttaacccgc
    17821 cgcgccgcgg cattggtaaa ccgctgtgtg attatctttc aacgatggca ccgcgtttta
    17881 ttatctactc cagctgtaac gcccaaacca tggcgaaaga tatccgcgaa ctgccaggtt
    17941 accgcatcga acgggtacag ctttttgata tgttcccgca caccgcgcac tatgaagtgt
    18001 tgacgctgct ggtgaagcaa taaaaaagcc acaggtgcgg cttcagattg ctgatcaggt
    18061 gcgctttgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa atcatgctaa
    18121 atcaatacat tgcagagttg atgtgggccg gataagcgca gcgcatcagg caatcttact
    18181 tttgtcatca gtctcaatta ctgcggaaac cactggtcat tgattttctg atatgtaccg
    18241 tcagctttaa ttgctgccag cgcgttattc agtttttcca gcagagcttt gttatccgga
    18301 cgtacagcga tacccaggcc ggtgccgaag tattgcggat cggtcacttt ttcagtcgca
    18361 acgcccagtt gcggattggt tttcagccat tcgtttacca ccgcggtgtc accaaatacc
    18421 ccatcaatac gaccattttt cagatcgata aaggcattct gataactgtc ataggagaca
    18481 gttttcactt ccgggtgctg atcctgaata tatttctggt gcgtcgtacc gttttccatc
    18541 ccaatgcgtt tgcctttcag gtcggcaaac gttttgtagg tatctttttt ggaaatcacg
    18601 acggctgagt tttcatagta gggtgtggta aacgaaacct gcttgctacg ctccggggtg
    18661 atatccatac cggagattac ggcgtcatat tttctgaatt tcagtgacgg gatcaggctg
    18721 tcgaacgcgt gattagtaaa agtacattct gcctgcattt gtttgcacaa ggcttttgcc
    18781 agatcgatat caaagccgac aatctcatta ttagcaccta tagattcaaa gggtggatag
    18841 gtggctgaaa cgccaaaatt gattttctct gcggcagaag caccgaaagt aaaggaagca
    18901 agtaaagcgg caagaactaa ctttttcatg atggaactcc cgtctgtcaa tcttatgatt
    18961 tttggccgtg tctgcggcat gggataacaa tgccatcaag tgaatttata tgcaataaac
    19021 atgattaaat aatttaaatg aaataaaaaa agacggataa cttagtgagt tgtccgtctt
    19081 cattataaga atttatgcac tatgtaggcc ggataaggcg tccacgccgc atccggcaca
    19141 ggcaccgtgc tgatgtctga tgcgacgctg gcgcgtctta tcagacctac aaaccccccc
    19201 ggcgaatgta cgcagctaca ttaattccgc cgttcgaatg ccagtgcttt gcgctcgatc
    19261 agacgcatca tcagcgtcag caggccgttg acgaccaagt aaataatccc tgccgcaccg
    19321 aacaccatta catcgtaggt gcgtccgtac aacaactggc tgtatcccat cacttccatc
    19381 agcgtaatgg tgtaagccag agaggtactt ttgaacacca gcaccacttc gttggaataa
    19441 gaagagagcg agcgtttaaa ggcatacggc agcaggatcg ccagcgtatc ttttttgctc
    19501 attcccaggg cgctacagga ctgccactga ctttctggga tcgcacgaat tgcaccgtaa
    19561 aacagctgcg tggtatacgc cgcactgttc agcgataacg caatcagcgc acataaccac
    19621 ggttctgaca acaaatgcca cagtgccgga tactcctgca acgtcggaaa ctggcccggc
    19681 ccgtaataaa tcaggaagat ctgcaccagc agcggcgtac cggtaaacag cgtgatataa
    19741 ccccgcacca gccacaccag caccggcgtt ttcagcgtca ggatgatggt aaaaatcaat
    19801 gccagaatca gtgccacaat cagcgaggca acggttagcg tcaggctggt gtgcagccct
    19861 ttcatcagct cgggtaaata ctcaaacatt agctgggcct ccgctcaaaa cgtgtcgcgc
    19921 gcaggtcaat gcgtttgaga atgtactgac tgagcagggt gatcaccagg taaatcgccg
    19981 ccgccacaat gtaccaggta aatggttcct gggtacgagt agcaatgctt ttggtttgta
    20041 acattaaatc attcacacta atcaaactga ccagcgcggt atctttcagc agcaccagcc
    20101 actggttacc gaggccaggc agcgcatgac gccacatctg cggcatcacc agacggaaaa
    20161 agatagccga tttcgacagc cccagcgcct gaccagattc ccactggccc accggtacag
    20221 ctttcaacgc gccgcgcagc gtttgcgagg cataggcggc atacagcagt gacagagcga
    20281 tgacaccaca aaggaacgga ctcacgtcga agttctcaat gtccatctgc accgggatct
    20341 gcacgaaccc aagattgata gtgaagccat ccgaaagcgt cagcagtaac tgcgaggagc
    20401 caaaatagat aaacagcact accagaattt ctggcaggcc acgcagaatg gttaccagcg
    20461 ctgaacctgc ccacgcgaca ggacgccatt tcgctgactc ccataccgca aagaacatcg
    20521 ccagcgccag cccgacaatc aatgcacaaa cggcaaggcc gacggtcatc ccggcggcgc
    20581 ttgctaaagg aaaaaattca ttcatcagga attacttctg gaaccatttg ttgtagatgg
    20641 tttcgtaagt gccatctttc ttcacttttt ccagcgcagt gttgagtttc tgctgcagtt
    20701 cagtgtttcc ctgacgtacc gcgatgccga ggccagtacc gaaataatct ttatcggtca
    20761 ctttatcgcc cactgccgcc agttttgggt tatctttcag ccactcagtg accactgcgg
    20821 tgtcaccaaa tacgctgtcg atacgcccgt tttgcagatc cagttttgcg ttctggtagc
    20881 tgtcatacgg aacggtggtg atttccgggt gcttatccat aatgaacttc tggtgtgtcg
    20941 tcccgttctg aacgccgact tttttgcctt tcagctgatc aacactggtg tatttgcctt
    21001 gctgacccac aaacagggca gagttgtcat agtacggggt agtaaacagt acctgctttt
    21061 cacgctccgg agtgatatcc atgcccgcca tcactgcttc tacgcgacgg aatttcaggc
    21121 ttgggatcag gctgtcaaac gcctggttag agaaagtaca ggttgcatca atctctttac
    21181 acagtgcctg cgccaggtcg acgtcaaaac caacgatctg gttgtttgca tcaatcgatt
    21241 caaacggagg atagggggct tcggtagcaa aacgaatggt ttcggcagct gtggcggaaa
    21301 gactaaaacc tgcaattaac gcggcaatca gaactttttt cattgttgtt atcccgaatc
    21361 ttagtgagag agatagtttt taaatgcttc ggtttgcggc tcggtaaagc agctcgcgtc
    21421 gccttgttct acgatatgac cattttccat atacaccact cggctggcgg ttttacgcgc
    21481 cacttcaact tcgtgggtga cgatcacctg ggtaatattc gtttctgcca gctcacgaat
    21541 gatgctgacg atttgtgccg taatttccgg gtccagtgcg gcggtcggtt catcgaacag
    21601 cagtacctgc ggttccatca tcaacgcacg ggcaatagca acacgctgct gctgaccacc
    21661 agaaagatgc agcgggtaac gatcgctata aggtttgaga cgcagacgtt ccagcagttt
    21721 ttctgcacgg gccagcgcct gatctttact caaccccagt acacggcagg gcgcttcaat
    21781 caggttttgc tgcacggtca gatgcggcca caggttgtat tgctgaaaca ccatgccaac
    21841 gttacgacgc aaatcgcgaa tcgctttgtc agagggtgtt ttggtgaaat cgaaatggtt
    21901 gcctgcaatg ttgagcgtac cggagcgcgg catctcaagc agattgagta cacgcagcag
    21961 cgagctttta cccgcgccgc tggggccaag taacaccagc gtttcgccct gtgggcaatc
    22021 cagcgtgata tcgaacagcg cctgatgcgc gccgtagaag caattaatgc cgtttaattg
    22081 aatactcatt gacactcgta tactggcagt ctgatagcta ttgaggtcga agatagtacc
    22141 tttaacagaa taattatgca atatttatgc tttaaaagtt aaaagcaaag cgcattattc
    22201 aataaacata gcacaaaata acgggggcgg tggtcggtga gcataaatgt cggcattcct
    22261 cacgaaatgc cggataattt acggggttta ttggttgatc aaggcgttag cgattctcga
    22321 tggactgacg gagcgtaccc gccgtggcat gaacgctacc gcctaagtag cgcacatcgt
    22381 cgatgaccca acactggcct tcctgaatca ttaacacttc atcctgccag ccctggtcac
    22441 cctgtttgag atccacccgc aatggaatgt tacgggcatc acgattaggg atagtcgatg
    22501 cactggcaac gtgggcgctg tctggcaagg tggttcgact ggagaacgga tcgctgctta
    22561 acaattcacg atgactatta tcgcggctgg catcgctaag ttgggttgcc agtttgtcac
    22621 tcaaataggg gcgcagggcg gtaatgtcat tgctgcgatg ttgaatgcgg tagtcgtaga
    22681 attgttgagc cacactgtca ggccctccct ggacacagga accggtgcgg gggccgttat
    22741 ctttataagc tggcgtgacg gtggtacagg cactcagcag cagtgcacat ggaataagta
    22801 gagatagtga acggtagcgc ataatcattt ccttattagc ttgctgtctg cgtcacacta
    22861 cgaccaaacg ctatcagatt atttaataca ttgtttttaa tacaattctg aaacaatctg
    22921 aacacctgat tgggtttgag tccattgctg gcgacccact caaggttgct cgtagctgac
    22981 ttacttaaat tacccaaaca atagacagtg taaaccattt gtgaggttga gcgtgttata
    23041 tggctgaaat gatcatttaa ggtaaatatg atagttctga aatggtggta taaatcctcc
    23101 tgtagaggtt aagagcatct gcaagaagcc agtttcacat ttataacgcg tgataaatga
    23161 atgcggttat tgtctaatca ttacaaaatt tttcacatgc gataagagat cctgcgatta
    23221 agatgattgc caaaattacg ttgtattttt gaaaatgagc gaaatttgat agtcccagct
    23281 ttg
//
LOCUS       DAZOYF010000052        22433 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZOYF010000052 DAZOYF010000000
VERSION     DAZOYF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.052"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            26..235
                     /gene="fumD"
                     /locus_tag="ACHUOF_001905"
     CDS             26..235
                     /gene="fumD"
                     /locus_tag="ACHUOF_001905"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR0611098.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            691..1317
                     /locus_tag="ACHUOF_001906"
     CDS             691..1317
                     /locus_tag="ACHUOF_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR0611099.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1338..3439
                     /locus_tag="ACHUOF_001907"
                     /pseudo
     CDS             1338..3439
                     /locus_tag="ACHUOF_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            3452..4090
                     /locus_tag="ACHUOF_001908"
     CDS             3452..4090
                     /locus_tag="ACHUOF_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR0611100.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            4154..4822
                     /locus_tag="ACHUOF_001909"
     CDS             4154..4822
                     /locus_tag="ACHUOF_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0611101.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4819..5604
                     /gene="phsC"
                     /locus_tag="ACHUOF_001910"
     CDS             4819..5604
                     /gene="phsC"
                     /locus_tag="ACHUOF_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR0611102.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5608..6420
                     /gene="ydhT"
                     /locus_tag="ACHUOF_001911"
     CDS             5608..6420
                     /gene="ydhT"
                     /locus_tag="ACHUOF_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR0611103.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(6432..7854)
                     /locus_tag="ACHUOF_001912"
                     /pseudo
     CDS             complement(6432..7854)
                     /locus_tag="ACHUOF_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(7980..8285)
                     /locus_tag="ACHUOF_001913"
     CDS             complement(7980..8285)
                     /locus_tag="ACHUOF_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR0611104.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8393..8469)
                     /locus_tag="ACHUOF_001914"
     tRNA            complement(8393..8469)
                     /locus_tag="ACHUOF_001914"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8433..8435),aa:Val,seq:gac)
     gene            complement(8474..8550)
                     /locus_tag="ACHUOF_001915"
     tRNA            complement(8474..8550)
                     /locus_tag="ACHUOF_001915"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8514..8516),aa:Val,seq:gac)
     gene            8858..10114
                     /locus_tag="ACHUOF_001916"
     CDS             8858..10114
                     /locus_tag="ACHUOF_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR0611105.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(10155..11528)
                     /gene="mdtK"
                     /locus_tag="ACHUOF_001917"
     CDS             complement(10155..11528)
                     /gene="mdtK"
                     /locus_tag="ACHUOF_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR0611106.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11743..12384
                     /gene="ribE"
                     /locus_tag="ACHUOF_001918"
     CDS             11743..12384
                     /gene="ribE"
                     /locus_tag="ACHUOF_001918"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR0611107.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12424..13572)
                     /gene="cfa"
                     /locus_tag="ACHUOF_001919"
     CDS             complement(12424..13572)
                     /gene="cfa"
                     /locus_tag="ACHUOF_001919"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR0611108.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13863..15074)
                     /gene="punC"
                     /locus_tag="ACHUOF_001920"
     CDS             complement(13863..15074)
                     /gene="punC"
                     /locus_tag="ACHUOF_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR0611109.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            15187..16119
                     /gene="punR"
                     /locus_tag="ACHUOF_001921"
     CDS             15187..16119
                     /gene="punR"
                     /locus_tag="ACHUOF_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR0611110.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            complement(16116..17141)
                     /gene="purR"
                     /locus_tag="ACHUOF_001922"
     CDS             complement(16116..17141)
                     /gene="purR"
                     /locus_tag="ACHUOF_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR0611111.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17440..17529
                     /gene="cydH"
                     /locus_tag="ACHUOF_001923"
                     /gene_synonym="ynhF"
     CDS             17440..17529
                     /gene="cydH"
                     /locus_tag="ACHUOF_001923"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR0611112.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            17695..18864
                     /locus_tag="ACHUOF_001924"
     CDS             17695..18864
                     /locus_tag="ACHUOF_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0611113.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(19010..19591)
                     /gene="sodB"
                     /locus_tag="ACHUOF_001925"
     CDS             complement(19010..19591)
                     /gene="sodB"
                     /locus_tag="ACHUOF_001925"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR0611114.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19719..20546)
                     /gene="mepH"
                     /locus_tag="ACHUOF_001926"
     CDS             complement(19719..20546)
                     /gene="mepH"
                     /locus_tag="ACHUOF_001926"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR0611115.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20881..21228
                     /gene="grxD"
                     /locus_tag="ACHUOF_001927"
     CDS             20881..21228
                     /gene="grxD"
                     /locus_tag="ACHUOF_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR0611116.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21342..21752)
                     /gene="tnpA"
                     /locus_tag="ACHUOF_001928"
                     /pseudo
     CDS             complement(21342..21752)
                     /gene="tnpA"
                     /locus_tag="ACHUOF_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            21775..>22433
                     /locus_tag="ACHUOF_001929"
     CDS             21775..>22433
                     /locus_tag="ACHUOF_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0611117.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SR"
BASE COUNT         5813 a         5215 c         5808 g         5597 t
ORIGIN      
        1 ttggcatgat aaataggtat aaagaatggg aaatcggaca aaagaggatg aactgtaccg
       61 agaaatgtgc agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg ggcaggaacc
      121 aaagcatatt gttatcgcag gcgtattacg tacagcatta gccaataaac gcattcagcg
      181 cagtgagctg gaaaagcagg cgatggaaac cgtcattaac gcactggtaa aatgaccatt
      241 caggatgagg cggtaagctt catcctgata ttcttgcttc agttgcggtc cagttaatca
      301 ttctaaataa caactgctga aattatacga atttcggtat gtattttaat ttatagcgat
      361 aatgttattt ttattttctg caatgaacgt cacaaatgat tgtcgcttaa gttaaattta
      421 tttcaaatga taataaatat cagttcgctt ataatttctt gctttgtaat tttaattgat
      481 aacgatcaat gttaagggct cagaataatc acattatttt ttctcctaac cataaaggat
      541 tagtttatcg gcgttaaatc ttttctcata cactatcggg ttagggaaaa tatctggtgt
      601 tggggacaga gtcagcagcg tattagttga atgcttaata tcaggctgct gaattgtcag
      661 cactgtgttt gcgaagagga tgcgaaataa atgaacccgg ttgatcgtcc actattagat
      721 attggcttaa cgcgattaga attcttgcgt atatcaggaa aaggccttgc tggtctaaca
      781 attgcccctg cgttactgtc acttttgggt tgcaaacagg aagatattga tagcggcaca
      841 gtagggttga taaatacacc caaaggggtg ctggtgaccc agcgagcgcg atgcacgggt
      901 tgccaccgct gtgaaatctc ttgtaccaac ttcaacgatg gctcagtagg gacattcttc
      961 tcccgtatca aaatccatcg caattatttc tttggcgaca acggggttgg ctctggcggc
     1021 ggcctgtatg gcgatctcaa ctataccgcg gacacctgcc gtcaatgcaa agagccgcaa
     1081 tgcatgaacg tctgcccgat tggtgcgatt acctggcagc agaaagaagg ctgtattacc
     1141 gtcgatcata aacgttgtat tggctgtagc gcctgtacta cggcatgccc gtggatgatg
     1201 gccaccgtaa ataccgaaag taaaaaatcc tcgaaatgtg tgttatgcgg tgaatgcgca
     1261 aacgcctgcc caacaggggc gttaaaaatt atcgagtgga aagatattac tgtgtgaatc
     1321 ttgcaaagga aaaaatcatg gctaacggtt ggacaggtaa tatattaaga gtcaatctca
     1381 cgacaggaaa tattaccctc gaagattcca gtaagtttaa aagttttgtc ggtggcatgg
     1441 gcttcggcta caaaattatg tatgacgaag taccgctagg cacgaaacct ttcgatgaag
     1501 cgaataaatt agtctttgct accggcccat tacctggatc tggtgccccc tgtagttctc
     1561 gcgtaaatat cacctcactt tctactttta ccaaaggaaa tttagtcgcg atgcccatat
     1621 gggtggcttt tttgcagcgc aaatgaaatt cgctggatac gacgtcatta ttatcgaagg
     1681 gaaggcgaaa tcaccggtat ggctgaagat taaagatgac aaagttagcc tggaaaaagc
     1741 cgatttctta tggggaaaag ggacgcgcgc aacgacggaa gaaatttgtc gattgaccag
     1801 cccggaaacc tgtgtggcgg ctattggtca ggctggggaa aaccttattc ctctctctgg
     1861 catgttgaat agccgtaacc acagcggcgg tgcgggaact ggcgcaataa tgggttcgaa
     1921 aaacctgaaa gcgattgcgg ttgaagggac gaaaggggtc aacattgccg atcgtcagga
     1981 gatgaagcgt ctcaatgatt acatgatgac tgaacttatt ggtgcgaata acaaccatgt
     2041 cgtgccaagt acgccacaat cgtgggcaga gtattcagat cccaagtcac gctggacagc
     2101 acgtaaaggg ctgttttggg gcgcggctga aggtggtccg attgaaacgg gtgaaattcc
     2161 gccaggcaat cagaatacgg tcggctttcg tacctataaa tccgtttttg acttaggacc
     2221 ggcggcagag aaatacacag taaaaatgag cggctgccac tcttgcccga tccgttgtat
     2281 gacccaaatg aatattcctc gggtgaaaga gtttggcgtg cccagcacag gtggtaacac
     2341 ttgtgtagca aactttgtcc ataccaccat cttcccgaac gggccgaaag attttgaaga
     2401 taaagacgat ggtcgtgtga ttggtaacct ggttggtctg aatttgttcg atgactacgg
     2461 cctatggtgt aactacgggc agttgcatcg cgactttact tattgttaca gcaaaggtgt
     2521 gttcaagcgt gttctgccag ctgaagagta tgcagaaatt cgctgggatc aactggaagc
     2581 gggtgacgtt aacttcatta aagattttta ctaccgtctg gcgcatcgtg tgggtgagct
     2641 gagtcacctg gctgatggtt catatgccat cgcagaacgc tggaatttgg gtaaagagta
     2701 ctggggctac gcgaaaaata aactctggtc gccgtttggc tatccggttc accatgccaa
     2761 tgaagcgtca gcgcaggtcg gttccattgt taagtgtatg ttcaaccgtg actgcatgac
     2821 gcatacccat atcaacttta ttggttccgg cttgccattg aaactgcaac gtgaagtggc
     2881 gaaagaactt tttggttctg aagatgctta cgatgaaacc aaaaactaca cgccaatcaa
     2941 tgacgcaaaa attaaatatg caaagtggtc gctgttgcgg gtctgtttgc ataacgccgt
     3001 cactctgtgc aactgggtct ggccaatgac cgtttcgccg ctgaaaagcc gtaattaccg
     3061 gggcgatctg gcgcttgaag ccaaattctt caaagcgatc accggcgaag agatgactca
     3121 ggaaaaatta gatttagctg cagagcgtat ctttacgttg catcgtgcct acacggtaaa
     3181 accgatgcaa accaaagata tgcgtaatga acacgatctt atctgttcct gggttttcga
     3241 caaggatccg cagatcccgg tctttactga aggtactgac aaaatggatc gtgacgatat
     3301 gcatgcttcg ctcacgatgt tctacaaaga aatgggctgg gacccacagc ttggttgtcc
     3361 aacccgcgaa acattgcagc gtctggggct ggaagatatt gccgccgatc tggcagcaca
     3421 caatctactg cctgcgtaag gaatgggtaa gatgaaccat caggatgaat tgccgttagc
     3481 gaaagtcagc gaagttgacg aggcgaagcg gcaatggttg caggggatgc gtcaccccgt
     3541 tgatacggta accgaaccag aacctgctga aatcctggct gagttcattc gccagcattc
     3601 tgcggctggg cagctggtgg cgcgcaccgt gtttctctcg ccaccctatt cggttgcaga
     3661 agaggaactg tcagttttgc tggaaagcat taagcagaac ggagactacg cggatatcgc
     3721 ttgtatgacg ggaagtcagg atgattacta ttactcaacg caggccatga gtgaaaacta
     3781 cgcggcgatg tctttgcaag ttgtggagca ggacatttgt cgcgccattg cacacgccgt
     3841 tcgttttgag tgccagacgt atcctcgtcc ttacaaagtc gccatgctga tgcaagcgcc
     3901 atattacttc caggaagcgc agattgaagc cgcgatcgcc gcgatggatg ttgcacctga
     3961 atatgccgat atacggcagg tggagtcatc gacggcggtg ttgtacttgt ttagcgagcg
     4021 atttatgacc tatggcaagg cttatggctt atgcgaatgg ttcgaggtag agcagtttca
     4081 gaacccctga aatttcaggg agatgtcatg gatagggtgg acagttgcag ccactgcatt
     4141 aggggataat cagatgtcct tcactcgacg caaatttgtt ctggggatgg gaacagtaat
     4201 cttttttact ggttctgcgt cttctctgtt agcgaacacg aggcaagaaa aggaagttcg
     4261 gtatgccatg attcatgacg agtcacggtg caatggctgt aatatttgcg cccgcgcatg
     4321 ccgtaaaact aaccacgtcc ccgctcaggg aagccgttta tcgatagcac acattccagt
     4381 caccgataac gacaacgaaa cgcagtatca cttttttcgc cagtcatgtc agcactgcga
     4441 agatgctccg tgcatcgacg tatgcccgac gggggcatcg tggcgtgatg agcaggggat
     4501 cgtgcgggta gaaaaatcgc agtgcatcgg ctgcagttat tgcattggcg cttgtccgta
     4561 cctggttcgc taccttaacc ctgtcactaa agtggcagat aaatgtgatt tctgcgctga
     4621 gtcccgactg gcaaaaggct ttccgcccat ttgcgtcagc gcctgtccgg aacacgcatt
     4681 gatctttggt cgcgaagata gcccagaaat tcaggcgtgg ttgcaggata ataaatacta
     4741 tcaatatcag ctacctggtg ccggaaaacc gcatctgtat cgccggttcg gtcaacattt
     4801 gattaaaaag gaaaatgtat gaacccgtcg caacatgctg aacagtttca gagccagtta
     4861 gcgaactatg tgccacagtt cactcccgaa ttttggccgg tgtggttgat cattgccgga
     4921 gtattgctgg ttgggatgtg gttggtgctg gggctgcacg ccttgcttcg tgctcgtggc
     4981 gtgaagaaat cagccaccga tcatggtgag aaggtttatc tttacagcaa agcggtcaga
     5041 ttatggcact ggtcgaatgc gttactcttt gtattgttgc tggcaagtgg gctgataaat
     5101 cactttgcga tggtgggcgc aactgcggtt aaaagtctgg ttgcggtgca tgaagtttgc
     5161 ggatttttgt tactggcatg ctggctcggc tttgtgctga tcaatgccgt tggggataat
     5221 ggtcaccact atcgcattcg tcgtcagggg tggctggaac gagcggcaaa acaaacgcga
     5281 ttttatttgt ttggcattat gcagggggaa gaacatcctt tcccggcaac aacacagtct
     5341 aaatttaatc ccttacagca ggtcgcctat gttggtgtca tgtatggatt gctgccgttg
     5401 ttactattga cggggctgtt gtgtctctat ccgcaagccg tgggagatgt gtttcctggc
     5461 gtaagatact ggttattgca ggcgcatttt gctctggcat ttataagcct cttttttatc
     5521 ttcggtcatc tttatctttg caccacgggg cgtacgccac acgaaacctt taaaagcatg
     5581 gtcgatggct atcaccggca ctaacgaatg atcattacac gagcagacct gcgagaatgg
     5641 cgtatcggag cggtgatgta tcgctggttc ctgcgccatt tcccacgcgg cggtagttat
     5701 gccgatattc gtcatgcact gattgaggaa ggatatacgg actgggcgga gagtctggtt
     5761 gagtatgcgt ggaaaaaatg gctggcggat gaaaacttcg cgcatcagga agtttcctct
     5821 atgcaaaaac tggccacaga ccccggagag ataccatttt gcagtcagtt tgcccgcagc
     5881 gacgatcatg ctcgcatcgg ctgttgtgag gataacgccc gaatcgcaac cgctgggtat
     5941 gccgcgcaaa ttgccagtat gggttatagc gtgcggattg gaagtgtcgg ctttaacagt
     6001 cacattggca gctccggtga acgcgcacgg gttgctgtaa ccggtaattc ttcgcggata
     6061 agcagcgctg gagatagtag tcggattgcg aataccggaa tgcgagtacg cgtttgtaca
     6121 ttgggtgaac ggtgtcatgt cgccagtaat ggtgacctgg tgcaaatcgc cagctttggc
     6181 gcaaacgcca gaattgccaa cagcggcgac aatgtacaca tcattgccag cggtgaaaat
     6241 tcaacggtag tgagcacggg cgtggtcgac tcaattattc tcgggctagg gggaagcgcc
     6301 gcactggcat atcatgacgg cgaaagagtt cgcttcgccg tcgccattga aggtgaaaac
     6361 aatattcgaa ccggagtccg ctatcgtctt aatgaacagc atcagtttgt tgagtgctga
     6421 tagctttacc tttaatcaaa cgaaagacgc cgacgagcac gggatgcagg ctttacgacc
     6481 gcccgagcca tcgcctcacc aatttctgca catgccgtaa gtccctgaac gaaaggctgg
     6541 tcgtgcatca accagggcaa cgcgccgaac gctacgcgtc cacgaatatc ttcgggttgc
     6601 tgtaacgtat aatcttcgcc aacatcaggg atttcagatc cccgcttcac gtaaggccag
     6661 tagtcgacga atagactctg aagggattgc cgcataatta tcgatgaaaa ccctggcaag
     6721 gcctttactg aaccgtttat ggtcgtgttc attcagatga ggaacaattt cctgtacggc
     6781 ttcatgcagg cgcagaatga cataacgcca gggaacagta tgtttttctc gtttattgcg
     6841 ttcgacttcc tggagatttt tttctgccca gtcaaatggg tcgcgttgtt tgcgctcggc
     6901 aaaccaggct tgagcaaagg aatcgacatt caggctctct aaggctatgc gttggctcca
     6961 gtctggatca gcaaacttga tttcctctac tatcaatcta aatacccgat ccaaaaggcc
     7021 ctcttcacct ttttgaatct cagcatttaa tgcctgatcg gtgacgatgt gtaagggctc
     7081 gtagggaata gggcaataga aatcggcttc gggtaaaata cccgtgcgcg acatcaacgt
     7141 aatatttagc ttttcacttg cgttatcgcg gtgaaaaacg acgtgttgtt tatcatcttc
     7201 aatgaacgaa ccatgctgaa tagccactgc cattgccgca tccagtccgc tcaaggatgt
     7261 tcccataata cccacgttac acgcatcgac ctttgcttcc atcaggcctg accacgggct
     7321 gggaaaatac gttctggttg cttcttcttc atcaggccag acgtgacccg tggcaatcac
     7381 cgctaaatca aacgtctcgc tgggtaaatc ctgatttgta gcgagcatga cgccagcatt
     7441 tgtaatttgc agatcggtaa cctggcatga ttcataaaca gccactgcaa atttttgctg
     7501 tcgtgcctgg tctactaatc gtaaaaattg atcgcggaaa tattcgccca gcagaattcg
     7561 cggtaaaaac tgacgatcgt gcaaggtttc ttttttaacg ccataacgct ggagatggct
     7621 ggcttcttgc ttttgtagcc attcgagata cgtacaatta atcggcggta tttcaatact
     7681 ggcaatattt gccagcatca tttttgagtt ttcctcatca ctgtatggca tcccgacacc
     7741 ggcctcgtca gcctgctcga agatagaaat agaaagtgga gtttgttgct gtagaagcga
     7801 gaataaggtg tagatccccg taggcccggc acccacaata gcaatttttt tcattgttac
     7861 ttacctgtac gcattctttc gaatttagtg ttatcagata agtttggttg agatttttag
     7921 aatttgagaa atttcagaca gggatgaaaa gggcgctaac cgcccttgat taaattctgt
     7981 caggcgagtt ttgcctgatt gatttgacta agtggctcat tgacatcgaa aactttggcg
     8041 acaacttcct caacgccgag atttttcagt cgggcagtat gtttttccag ataggcaagg
     8101 gcgctttttt catcagtgaa aaggtagatc ccaccggctt cgtggttctt ttcactttct
     8161 gtccatacct tccacagaaa accaggttcc tgattaatcg actcagcaag tggtttaagc
     8221 tgctcagcca ttgcgtcgcc aaatgggcca ttaaaagcaa aatgaagttg taacaaagtt
     8281 gccatagcga ttctccttct gataacgcat acgaagacag tgtaaatctt ttaacgtatt
     8341 ggatacagag aagaaacaga gggaaaaagg aaaagaccag attaagaaaa tctggtgcgt
     8401 ccgagtggac tcgaaccacc gacccccacc atgtcaaggt ggtgctctaa ccaactgagc
     8461 tacggacgca ggatggtgcg ttcaattgga ctcgaaccaa cgacccccac catgtcaagg
     8521 tggtgctcta accaactgag ctatgaacgc aacgttgtag gtgacaacgg ggacgaatat
     8581 tagcggcaga gtggaaaggt ggcaagaggc aaaacgtaat tttctgcgct atttcgaccg
     8641 tttgcagagc ttttaagcaa attggctata ttttgttgat ttgcaagggc gatttttatt
     8701 caggatcgca tttacatctg atacaaccca ataattcgtg tatcagagtt taaacagagt
     8761 tgtttgccac catctcaaat ctgtcgtgga taaatcctcc gtagccttta cacttaggta
     8821 gcaataaagg cacacagatg cagcaaagga ataaatcatg ggatctgatg cgaaaaactt
     8881 gatgagcgac gggaatgtgc aaattgttaa gaccggcgag gtccttggcg cgacgcaact
     8941 tactgaaggc gagttgattg ttgaagctgg cggaagagcc gaaaataccg tggtcacggg
     9001 ggctggctgg ttgaaagtgg caaccggtgg gatcgccaaa tgcacacagt acggtaacaa
     9061 tggcacgcta tcggtcagcg acggtgccat tgccacagat attgttcagt ccgagggagg
     9121 cgcaattagt ctctctacgc tcgctacggt taatggccgc catcccgaag gtgaattcag
     9181 cgttgatcag ggttatgcct gcggtttgtt gctggaaaat ggcggtaacc tgcgtgtact
     9241 ggaaggacat cgcgcggaaa aaatcattct cgatcaagag ggcggcctgt tggttaatgg
     9301 gacaacctca gcggtcgtgg tagatgaagg tggtgaattg ttggtgtatc caagtggaga
     9361 agccagcaat tgtgagatta atcagggcgg cgtttttatg ctggccggga aagccagtga
     9421 tacgttgctt gctggtggca ccatgaataa tctcggtggt gaagactctg acactattgt
     9481 tgagaatgga tccatttatc gtctggggac ggatggcctt cagctttaca gttccggtaa
     9541 gacgcaaaac ctgtcggtga atgtgggggg gcgggctgaa gtgcatgccg gtacgctgga
     9601 aaatgcggta atacaaggtg gaacagtgat cctgttgtca cccaccagcg cggacgaaaa
     9661 ttttgtcgta gaggaagatc gcgcaccggt tgaactgacc gggagtgttg cattactgga
     9721 cggcgcttca atgattattg gttatggcgc agagctgcaa caatcaacga ttactgtaca
     9781 gcagggcggt gtgttgattc tcgacggcag tacggtaaaa ggtgacagcg tcactttcat
     9841 tgttggtaac atcaatctga atggcggaaa actgtggctg atcactggtg cggcaacgca
     9901 tgtgcaactg aaagtgaaac gcctgcgcgg agagggagcg atttgcctgc aaaccagtgc
     9961 gaaagaaatt tcacctgact tcatcaatgt gaaaggggaa gttaacgggg atatacgcgt
    10021 tgagataaca gatgccagtc ggcaaactct gtgtaacgct ctgaaattac agccagacga
    10081 agacgggatt ggcgcaacgc tccagcctgc gtaaaaacac aggcgcaacc cgtgaaggtt
    10141 gcgccttgtc tttattagcg ggacgctcgt tgcagaatga tggctgacgg catacgttgc
    10201 aggaaccgca tacgcagcat catcataatg gctgccgatg tcaggccgat aataaagcct
    10261 atccagaagc ctgctggccc cataggttca acgaccagat cggtcagtgc cagaatatag
    10321 ccgcttggca agcccagcac ccagtaagct gtaaaggtga tatagaaaat ggaacgcgta
    10381 tctttataac cacgcaaaat cccactgcca atcacctgga ttgagtcaga aatctgatat
    10441 accgccgcca gcaacatcaa atgcgcagcc agcgttacaa cctcgggatt gtcgttgtac
    10501 aacagggcga tttgctcccg cagtgaaacc gtgaaaatgg ccgtcagggt tgccatacag
    10561 acacccacca taagcccggt ccgcgcagcg gtttgcgcat ccagcgttga gccctgaccc
    10621 agacgataac ctacgcggat agttaccgct gccgccagcg acattggaag cacaaacatc
    10681 agtgaactaa agttcagggc aatctggtgt cctgcgacat caacaatacc gagcggagac
    10741 actaacagag ccacgacggc aaacagtgtc acttcaaaga acagtgccag cgcaatcggc
    10801 aaaccgagtt gaatcagtcg tttcataacc gcaggctcgg gcttagcggt gcctttttcg
    10861 ttacgaatat cgcgcatgga gcgggcgcgt ttaatgtaag aaaccatggc aaggaacatg
    10921 acccaataca ccgccgcagt agccacgcca caaccaacgc caccgagctc aggcataccg
    10981 aaatgaccat aaataaagat atagttcacc gggatgttca ccagcaggcc gataaagccc
    11041 attaccatac ccggcttggt ttttgccaga ccttcacatt ggttacgggc aacctggaag
    11101 aacagatatc ccggcgcgcc ccacaacaac gcacgcagat aacccacggc tttgtccgcc
    11161 agagccggat cgatgttttc catgtagcgg ataatgtaac ctgcattcca cagcaccagc
    11221 ataatgagaa cggaaacaaa acctgccagc cagaaacctt gtcgcacctg atgcgcaatg
    11281 cgctcacgtc gaccggaacc atttaattgc gcgataaccg gcgttaatgc cagcagcagt
    11341 ccgtgaccaa agaggatcgc cggaagccag atagaagtac cgatagcgac cgccgccatg
    11401 tcggtggcac tatagccgcc cgccatcacg gtatcgacaa aacccatcgc agtttgggcg
    11461 atttgcgcga gaatcaccgg gattgctaat gctaataaca gacgcgcttc actgatatac
    11521 ttctgcacgt gaacaccttt tatttgtagt tatatgagag actaaaaagc cgcctgaatg
    11581 ggcagcaaaa agaagaagca ggggaaattc agtctattgt agcgaggatt tattaattct
    11641 ccagtgaaaa aatagccatt cttacccctt cactggtaag ggcgttttcc acctgctatt
    11701 gtgctgaaca gaatgtctta actgatttca ggagttgtaa gtatgtttac ggggattgta
    11761 cagggcaccg caaaactggt gtcgattgac gagaaaccaa attttcgtac gcatgtggtg
    11821 gagttacccg accacatgct ggacggcctg gaaaccggtg cttccgtggc gcataacggt
    11881 tgctgcctga ccgtgacgga aattaacggc aaccatgtca gttttgacct gatgaaagaa
    11941 acgttacgca ttaccaatct tggcgattta aaagtggggg attgggtaaa cgttgagcgt
    12001 gcggcgaaat tcagtgatga aattggcgga cacttaatgt caggtcatat tatgaccacc
    12061 gctgaagtgg cgaaaatatt aacctcggaa aataatcgcc agatctggtt taaagtccag
    12121 gatagtcagt tgatgaaata tatcctgtac aaaggattta ttggcatcga cggtattagc
    12181 ctgaccgtcg gcgaagtcac gccaacgcgt ttttgcgtcc atttaattcc ggaaacactg
    12241 gaacgcacga ctcttgggaa gaaaaaactt ggcgcacgcg tcaacattga aatcgatcca
    12301 caaactcagg cagtggtaga tacggtagaa cgtgtgctgg cggcacgaga aaatgccatg
    12361 aatcaaccag gtacagaagc ctgatctgaa ctcgcccccg aagagggggc gatagaatag
    12421 cctttagcga gcgactcgaa ggccgttttc aacaccgcgt gagaacacca cctgccagag
    12481 ctgaatatca cgggcgcgga aagcacctgc gcaggcattc agataatagg taaacatccg
    12541 tttaaagcgt tcactgtagt tatccgcaat ttctggccat gcggcgagga atcgttcata
    12601 ccacgccatc aacgtagtat cgtaatcagc accgaagtta tgccagtctt ccatcacaaa
    12661 gtggggttcg ctggattgag caatctggcg tacagagggc aggcaaccgt tcggaaaaat
    12721 atatttatta atccagggat caacattcag atcggttttt ttcgaaccga tagtatggag
    12781 caggaatata ccttccggtt tcaaattacg atccaccacc gcaaaatagg tatcgtaatt
    12841 tttcggtccg acgtgctcga acatccccac agaaacaata cgatcaaact ggtcgttcag
    12901 gtcacgataa tcttgcagca aaatggtgac atccaggcct ttacagcgtt cctgagccat
    12961 tttttgctgt tcggcagaaa tggtgacgcc gaccacgctt acgtcatagt tagatgccat
    13021 gtagtgcgct agtccgcccc agccgcagcc aatatccagt acgcgcatcc ctggttttaa
    13081 ctgcaatttt tcacaaatca ttttgagctt cgcctgctgg gcagattcca gattatcggc
    13141 atctttccag taagcgcagg aatattgcat gaagggatca agcatgcggc tgaacaagtc
    13201 attacccaaa tcgtaatgct ccttgccgac tatccaggca cgttttttac tctgcagatt
    13261 gaagagacga gcgccggcaa tacgcagcgt gtctttgaaa tgatggggga gttggttctc
    13321 gagacctgcg cgtaagactt tgctaaaaaa catatccagt cggtcacatt cccaccagcc
    13381 atccatataa ctttcgccta accccaaaga gccttcctgc agaacgcgtt taaaaaaatc
    13441 ggggtttttc acacgaatat ccgccggggc agaaccgtta atggctatac cggcacggct
    13501 aagtaattcg ttggcgatac ggtaccagtt gtcatccggt acactgactt cttctataca
    13561 cgatgaactc atagtttctc catcactggt cggtgatcag aaccttaaaa cagcgtagac
    13621 gctttttggg ctttgtgaga aatctcgcag aaaaaaccgc gagtctataa tccgacgtag
    13681 aacagaggga aaggaggaac ccttgccgaa atgaccatcc atagtgagtc gggaacgatc
    13741 ctgtccccgt aaaaacataa tatttatcgt agctttaaca actcgaaatt agtataggcc
    13801 gctaatttca atcatacaac aaaattttgt tagcagccta agtataagta tatcgatata
    13861 gatcagtgtg attcgctatg agcgacttcg gcattgccat gattcgggca gtcaacttct
    13921 tcacaacgtt gcatcatgta acccagcgcg accagcacta ctgttgataa catcacgctg
    13981 gtggtggtga gcaatggcgt gctgatacta ataagccagg aaactaccag acttgcgagg
    14041 aagcacagac ccagttgcag agtgttctgc aacgctgcgg cgcgaccggt tgcatgtggg
    14101 aagggacgca gcgcctgggc gacaacaata gggtagatcg cgccattggc aatcgccatc
    14161 acacagaatg ggatcaggat ttcgaccagc gacacatggc taatgaagcc cgcagcccag
    14221 gtcgcaatga cgctgacagc aaacagcacc agcaaccacg gtaataactg cttgccttgc
    14281 catttctgca gtgcggcgcg acagccataa ccaccaatca gaaacgcgat agtttgcggg
    14341 acataactta aaccaataac tgccgggctg tagcccattt cactaaggat gaacggtgaa
    14401 ccggtcagcc atgcaaaaaa actggctgaa caggctgcgt atatcagcac gttgccgcga
    14461 taggttttag aacgtagcag gtcggtaaag gtcagaccat cctgactatt gttacgggcc
    14521 ttcgtcgtgg gtttgagcca gaaaataggc agaatcagca ccacggtaat ggcaaacagg
    14581 gtggcgaaaa tcgcctgcca ggaaaaatgg accagcagcc agcttcctaa caggggagcc
    14641 agtgccggag acagacccac cagcggcatg atggtcgcaa aaatacggtt gactttctgt
    14701 gaaggataat aatctgtcac taatgcttgc cagataaccg ccgcggcgca gacccccaca
    14761 gcctgtacaa aacgcaatac cagcagcgtg gcggcgtttt ctacccacag catccccaga
    14821 ctacctaacg caaaaattgt caggccgatt aataacaccg gtttacgacc ataacggtcg
    14881 gagagtggcc cccacagaag ctgggctgcg gcaaaaccgg caaggaacag actaaggctg
    14941 gcactgacag cagacgcagg cgtttgcagg tcggcctgta tggcggcgaa agcaggcaga
    15001 tacatatcgg ttgccagaaa accgagtacg ctcaaacccg ccagccagac taaaaatctt
    15061 ttcccaggtt gcatgtatat tctctaaatc tctcaattaa acaccgccgc aaagtgtagg
    15121 gagtgaaatt agtgttgtga aacggtaata tttgatgact ggtttcaaaa attttgcagg
    15181 cagactatgt ggtcagaata ttcactcgaa gttgttgatg cggtagcgcg taatggtagt
    15241 tttagcgctg cggcacagga gctgcatcgt gttccttctg cggtcagcta taccgtgcgt
    15301 cagctggaag agtggctggc ggtaccgctc tttgaacgac ggcaccgtga tgtggagctg
    15361 accgctgctg gagcgtggtt tctcaaagaa gggcgctctg ttgtcaaaaa aatgcagatc
    15421 acccgccagc aatgtcagca gatagcgaac ggctggcgcg gtcagttagc tatcgcagtg
    15481 gataatattg tcaggccaga acgtacacgg cagatgatcg ttgattttta tcgccatttt
    15541 gatgatgtcg aacttcttgt ctttcaggaa gtgttcaacg gtgtctggga tgcgctttcc
    15601 gacgggcgcg tggaactggc aattggcgca acacgcgcga ttccggtagg cggtcgttat
    15661 gccttccggg atatggggat gctaagctgg agttgcgttg ttgccagtca ccacccgctg
    15721 gcgttgatgg atggcccgtt cagcgatgat acgttgcgca actggccgtc gttggtgcgc
    15781 gaagacacct cgcgaacgct acctaaacgt attacctggt tgctggataa ccaaaaaaga
    15841 cttgtcgtac ctgactggga atcatcggca acctgtatct cggcaggatt atgcataggg
    15901 atggtgccaa cacatttcgc caaaccgtgg cttaatgagg ggaagtgggt agcacttgaa
    15961 ctggaaaacc ccttcccgga ttcggcatgt tgtctgacat ggcagcaaaa tgatatgtcg
    16021 cctgcgttaa cctggctgct tgagtatctc agcgatagtg agacgctgaa taaggagtgg
    16081 ctgcgggagc cggaagagac tcccgcaacg ggtgattaac gacgatagtc gcggaacggg
    16141 ccgtcagcca cggagcggcg ttcaatcaaa cgcggatgca cttcaatgga ctgcggttct
    16201 tcacgtttgt tgacgatacg atccaacagc atgttgaacg ctgtttcacc cagcgaatct
    16261 tttggctgat ggatcgtggt cagcgccggc gtaaaatagc gcgcgttgcg cacgttatca
    16321 taaccgatca gcgaaacatc ctgcgggacg cgcaggccca tttcatcagc agcacaaagt
    16381 gcgcccattg ccatgatatc gccaccacag aagacggcag tagggcgatg cggctgcgac
    16441 aggatttgct gcatggcgcg ataaccggat tcaggttcaa agtcaccctg cacaatccag
    16501 ctttccggca ccttgatcat cgcttcttcc atcgccttca taaaaccggc aaggcggcct
    16561 gcgccggtgt tacgttccag cgggccgggg atgacgccga tttcgcggtg accgcgttca
    16621 atcagataac gcccggccat gtagccgcct tcgaacgcgt tatcaatgac cgcatcggtg
    16681 aagtcagctt ttgcttcacc ccagtccatc accaccattg ggatatggcg atactcttcc
    16741 agcatcgcca gcaacggctc tgggtactca gaacacatca ccagcagacc atcgacgcgt
    16801 ttttgcgcca tcatcgacag ataagcccgc tgtttctcaa gattgttcca cgcattaccc
    16861 agaatcaggg tgtaaccttt ctggaagcaa tttttttcaa ctgcttcaat gatctcggca
    16921 aaataggccg cttcgctgct ggtcgccagc aaaccgatag acttggtgtg gttaaccttc
    16981 aggctacgcg ccaccgcgct aggggagtag tgtaattctt taatcgctgc ccacacggcg
    17041 ttgcgcgttt cttcagcgac gaaacgtgtt ttgttgatca cgtgtgacac agttgtagtg
    17101 gaaacgtttg ctcgtttcgc tacatctttt attgttgcca ttccatttca ctccagaccc
    17161 taacttcagc tcctgcaaaa tcgcaaggta aacgtttgcc tttacacacc ttacgcacaa
    17221 caaaagtgtt gcggtacgcc ggaaaaacga cacggagcgt caggaagggg tcaatggcca
    17281 gtacgcaaat agtgtaagcg tggaattttg gccgatcttg acgaaaaggg gaagtggtaa
    17341 aaacacttat cattataaat gacgggagat tttttcatca cagtgtgtaa aaatgcgtaa
    17401 tatcactttt tgtgtggtaa taaaaaagga gaaaacttga tgagcaccga ccttaaattt
    17461 tcactggtaa caacggttat cgtcctcggt ttgatcgtag ccgtgggttt gactgccgcg
    17521 ctgcactgat ttcctctcga gggagcgcgt tctccctcgc atctccttcc tgtttgttag
    17581 caaaatggca aaagtgtttt ctctttttgt tattttcatt tttttgtgat tgctgtcatg
    17641 ctttcgccac tttaatgaat atcgcgtaat gacgcgaaga ggtggagata aggcatgaaa
    17701 attaactatc cgttgctggc gctggcgatt ggcgcgtttg gtatcgggac aacggagttc
    17761 tcgccaatgg gcttgttgcc cgtcattgcg cgcggtgtgg atgtctcgat tcccgctgcc
    17821 ggaatgttaa tcagtgccta tgcagttggc gtaatggtgg gcgcgccgct gatgacgctt
    17881 ctactttctc atcgtgcccg ccgcagtgcg ttgattttcc tgatggcaat tttcacgcta
    17941 ggcaacgttc tttccgccat cgcgccggat tatatgaccc tgatgctttc acgtattttg
    18001 accagcctga atcacggagc attttttggt ttgggttcag tcgtggccgc aagcgtggtg
    18061 ccaaaacata aacaggccag cgcagttgcc actatgttta tggggttaac cctggcaaat
    18121 atcggtggcg tgccggcggc gacctggttg ggtgaaacca tcggctggcg gatgtcattt
    18181 ctggcaacgg cggggctggg agtgatttca atggtaagtc tgttcttctc attacctaaa
    18241 ggtggtacag gggcacgacc tgaagtgaaa aaagagctgg cggtattaat gcgtccgcag
    18301 gtgctgtctg cattgctgac gacggtactg ggagctggtg caatgtttac cctctacacc
    18361 tatatctctc cggtactgca aagtattacc cacgcaacac cggtgttcgt cacggcaatg
    18421 ctggtgctga ttggtgtcgg attctctatc ggtaactatc tcggcggcaa actggcagat
    18481 cgttcagtta acggcacgtt gaaaggcttt ttgttgctgc tgatggtgat tatgctggca
    18541 atcccgttcc tggcccgcaa tgagttcggc gcagctatta gcatggtggt gtggggcgca
    18601 gcaacctttg cggttgtacc tccgttacag atgcgcgtga tgcatgtcgc cagtgaagcg
    18661 ccaggtctgt cttcatcagt caatattggt gcctttaatc ttggaaatgc gctgggagca
    18721 gctgctggtg gtgcggtaat ttccgctggg ctgggataca gctttgtgcc ggtgacgggg
    18781 gcgattgtcg cgggactggc attattgctg gtgtttatgt cagccagaaa acaacctgaa
    18841 acagtttgcg ttgctaacag ctaattaaac aaaaacgcag aaggataacc ttctgcgtta
    18901 cagttgctga caacgtgcga tttatcttgc cgaatgcgct gttaacgcct catccgcgtt
    18961 ggcatggtaa gcgaagcgca tcaggcaatg ctgcatttgc catcagttat tatgcagcga
    19021 gatttttcgc tacgaattcc cagttcacca gcgcccagaa gtgctccaga tagccaggac
    19081 gtgcattgcg atagtcgatg taataagcgt gttcccagac atcaacggtc agcagcggag
    19141 tcgcatcggt ggtcagcgga gtacccgcgt tagaggttga aacgatagcc agtttgccat
    19201 cgctgttttt caccagccag gtccagccag aaccaaagtt tttgatcgct gcatcagtaa
    19261 actgcgcttt gaaatcggca aagctgccaa aagatgcggc gatagcttca gcgacttttc
    19321 cagtcggttc gccaccggcg ttcggtgcca ggcagttcca gtagaaagta tggttccaga
    19381 cctgagctgc gttgttgaat acgccacctt cagagctgcg aataatctct tccagtgatt
    19441 taccttcaaa cgcggtacct ttaatcaggt tgttcaggtt agtgacataa gtctgatgat
    19501 gcttgccgta gtgatactcg atagtttccg cagaaatgtg cggtgccaga gcatctttag
    19561 catatggtag tgcaggtaat tcgaatgaca ttgctactct cctttattat taatttgcat
    19621 acgtacaata gccttattgt gcgtatggat agggtagcaa attaaaaggt gagacaaaag
    19681 atgaacttac cctgttgccg taacaacagg gtaaagtttt agcgaagtgt ttttggggtc
    19741 attacccgac gagcgccaac atagtggcgc tgccagtagt cttcactgag agaagtgatt
    19801 tgaatttcct gacctgtgcg cggtgactga ataaatttgc cgttgccgac atacacgccg
    19861 acatgatcgg ctgtaccgcg tccctgagta cggaaaaaga ccaggtcgcc gtttttcagc
    19921 tcactacgtt cgataggtcc tgcatcacgc aggtgataca tttcattcgc cgtacgcgga
    19981 atacgaattt tcaccaaatc tttataagcg taataaacca ggccgctgca atcaaaaccg
    20041 gtacgcggtg agctgccgcc ccaacgatat ggcttaccaa tttgctgcat cagtttattc
    20101 attgccactt tcgtggcttt ttgcaccttc gctttgtgcg cgtcggcaag ggttcctgac
    20161 gcggcatttt tgactttcac acaatgcgat ttatagccct tacgtttggt acacttttcg
    20221 gtaaagctgg ctgaggccgt tttattgacg gcgtgtttgc tgcgagtttt ggcgttttta
    20281 gaagcggtaa tggaagattt tttaacagaa gaagcggttt tgcttttggt catactttta
    20341 ctggcggctt ttttcgctgt tttctggctg gctttctggg tctttttggt ggttgccgtg
    20401 cttttctttt tatctgcttt tttggtgata tgagtggtag cagaactctc cctggcttgc
    20461 tttgaagcat gggccatagg cgttaaaggc agggtggtaa aaagtaaagc acagagcgtg
    20521 atcgaaatac ggtttatccg cgccactgag cgtccctctg ttaaggcagg tctatcatgc
    20581 ctgcgtctga tttacaaaac ttgatgattc taatgcatag aagaagagaa agttaatagc
    20641 ttttattgtt aaaaaatacg tttcgttggc gagcgcaaaa aaatgcagca gtgaaagcta
    20701 aaaatggtta taaatacgcc agtttgccgc gcatcagaga agaaacgaat atttgaaatg
    20761 caacaaatag agcaaaggga taaaatagca aaagcgtgat gaaaatgtta ttttccgatg
    20821 ccgcactgac ccgctacaat gtcagattac tgccgtaaag aagtttgagg aagcaagaca
    20881 atgagcacca ctatcgaaaa aatccaacgc cagattgctg aaaacccgat cctgctgtac
    20941 atgaaaggtt caccgaaact gccgagctgc ggtttctctg cccaggcagt ccaggcgctt
    21001 gccgcatgtg gcgaacgttt tgcctatgtt gatattctgc agaatccgga cattcgtgcg
    21061 gaactgccga aatatgctaa ctggccgacc ttcccgcaac tgtgggttga cggcgagctg
    21121 gtcggcggtt gtgatatcgt gatcgaaatg tatcagcgtg gcgaactgca gcagctgatc
    21181 aaagaaactg ccgctaaata caagtctgaa gagccggacg cggaataatc cgtcctgcaa
    21241 tactgacgga tgcgaacggg catccgtcag tcctttatcc ggtgcgccgt aaaacctcgt
    21301 ccttcagggc ggggatataa ggcgcggttt tccacctgac caggtgtttg ctgttgttca
    21361 atgtactggc ggatgacgga tattggcgca ccttcgcagc tactggcaaa atagccagga
    21421 ctccacaaaa cgcctttgta gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt
    21481 aacctattcg atatgccttt gaggctgttt accagactgg atatcgccag tttgggagga
    21541 tagttaatta acaaatggac gtgatctggt tcgccatcca tttcaaccag ttcagctgca
    21601 aaatcagcac atacatttga aaagtaagtg cgtagttttt ctgtcgtgtc atggtcaaaa
    21661 atctggcgtc ggtatttggt gacaaagacc aggtgaacat gcatcaggaa aacacaatgc
    21721 cttccacgcc ggatatcggt ttcttttttc acagaccaaa gtatcatttg acctgtgaaa
    21781 cgattacagg catttaaatt ccagttaaga cccggtggtc aacaggagcg tgaaatgagg
    21841 cgcttcgcag gcgcatgtcg tttcgttttc aatcgtgctc tggcgcttca gaatgagaat
    21901 catgaggccg gtaataaata catcccttac gggaaaatgg cttcctggct ggttgagtgg
    21961 aaaaacgcca ctgaaacgca atggcttaaa gattcgccct cacagccatt gcaacagtca
    22021 ctgaaagacc ttgagcgggc ttacaaaaac ttcttccaga agcgggctgc ttttccccga
    22081 ttcaaaaagc ggggacagaa tgctgcattc cgctacccgc aggacgttaa gctcgatcag
    22141 gaaaacagcc gtatttttct gccgaaactt ggctggatgc gctaccggaa tagccgtcag
    22201 gtcacgggag ttgtgaaaaa tgtcactgtc agccagtcct gcggtacgtg gtacatcagt
    22261 attcagacag aaagtgaagt atcaactccg gttcaccctt cagcatcaat gatcgggctg
    22321 gatgctggcg tggctaaact cgccacgctg tcagatggca cagtctttga gcctgtaaac
    22381 agttttcaaa aaaaccagaa gacgctggcg agacttcagc gacagttaag ccg
//
LOCUS       DAZOYF010000053        22418 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZOYF010000053 DAZOYF010000000
VERSION     DAZOYF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.053"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            82..2682
                     /locus_tag="ACHUOF_001930"
     CDS             82..2682
                     /locus_tag="ACHUOF_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFR0611118.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3742
                     /locus_tag="ACHUOF_001931"
                     /pseudo
     CDS             2673..3742
                     /locus_tag="ACHUOF_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3754..4294
                     /locus_tag="ACHUOF_001932"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="ACHUOF_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="ACHUOF_001933"
     CDS             4302..4817
                     /locus_tag="ACHUOF_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0611119.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="ACHUOF_001934"
     CDS             4783..5520
                     /locus_tag="ACHUOF_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR0611120.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHUOF_001935"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHUOF_001935"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR0611121.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNLLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="ACHUOF_001936"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="ACHUOF_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR0611122.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHUOF_001937"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHUOF_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR0611123.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHUOF_001938"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHUOF_001938"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR0611124.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="ACHUOF_001939"
     CDS             10827..12734
                     /locus_tag="ACHUOF_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0611125.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHUOF_001940"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHUOF_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR0611126.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHUOF_001941"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHUOF_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR0611127.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHUOF_001942"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHUOF_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR0611128.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="ACHUOF_001943"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="ACHUOF_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR0611129.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHUOF_001944"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHUOF_001944"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR0611130.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="ACHUOF_001945"
     CDS             complement(17402..19162)
                     /locus_tag="ACHUOF_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0611131.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="ACHUOF_001946"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="ACHUOF_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR0611132.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHUOF_001947"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHUOF_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR0611133.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHUOF_001948"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHUOF_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR0611134.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..>22418
                     /gene="sxy"
                     /locus_tag="ACHUOF_001949"
     CDS             22005..>22418
                     /gene="sxy"
                     /locus_tag="ACHUOF_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR0611135.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGR"
BASE COUNT         5723 a         5257 c         5876 g         5562 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatct gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 tgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tgggtaga
//
LOCUS       DAZOYF010000054        22278 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZOYF010000054 DAZOYF010000000
VERSION     DAZOYF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.054"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1025)
                     /locus_tag="ACHUOF_001950"
     CDS             complement(<1..1025)
                     /locus_tag="ACHUOF_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0611136.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="ACHUOF_001951"
     CDS             complement(1127..2107)
                     /locus_tag="ACHUOF_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR0611137.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="ACHUOF_001952"
     CDS             complement(2115..2825)
                     /locus_tag="ACHUOF_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0611138.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHUOF_001953"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHUOF_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR0611139.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="ACHUOF_001954"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="ACHUOF_001954"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR0611140.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="ACHUOF_001955"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="ACHUOF_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR0611141.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="ACHUOF_001956"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="ACHUOF_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR0611142.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="ACHUOF_001957"
     CDS             9402..9512
                     /locus_tag="ACHUOF_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR0611143.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHUOF_001958"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHUOF_001958"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR0611144.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHUOF_001959"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHUOF_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR0611145.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(11931..12097)
                     /locus_tag="ACHUOF_001960"
                     /pseudo
     CDS             complement(11931..12097)
                     /locus_tag="ACHUOF_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12394..13026
                     /gene="yihA"
                     /locus_tag="ACHUOF_001961"
     CDS             12394..13026
                     /gene="yihA"
                     /locus_tag="ACHUOF_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR0611146.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHUOF_001962"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHUOF_001962"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR0611147.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16558..17490
                     /locus_tag="ACHUOF_001963"
     CDS             16558..17490
                     /locus_tag="ACHUOF_001963"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR0611148.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17531..18961)
                     /locus_tag="ACHUOF_001964"
     CDS             complement(17531..18961)
                     /locus_tag="ACHUOF_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR0611149.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLAYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19116..19742)
                     /gene="dsbA"
                     /locus_tag="ACHUOF_001965"
     CDS             complement(19116..19742)
                     /gene="dsbA"
                     /locus_tag="ACHUOF_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR0611150.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19759..20745)
                     /gene="srkA"
                     /locus_tag="ACHUOF_001966"
     CDS             complement(19759..20745)
                     /gene="srkA"
                     /locus_tag="ACHUOF_001966"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR0611151.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20822..21091)
                     /locus_tag="ACHUOF_001967"
     CDS             complement(20822..21091)
                     /locus_tag="ACHUOF_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR0611152.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21161..21745
                     /gene="mobA"
                     /locus_tag="ACHUOF_001968"
     CDS             21161..21745
                     /gene="mobA"
                     /locus_tag="ACHUOF_001968"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR0611153.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21727..22254
                     /gene="mobB"
                     /locus_tag="ACHUOF_001969"
     CDS             21727..22254
                     /gene="mobB"
                     /locus_tag="ACHUOF_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR0611154.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5515 a         5584 c         5333 g         5846 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcactggcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacactcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg aggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga
    16441 taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg
    16501 ttatttgggt cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg
    16561 gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg
    16621 agtatcgtat tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg
    16681 attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat
    16741 tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg
    16801 caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt
    16861 aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg
    16921 atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc
    16981 tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg
    17041 gagcgacgtg gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat
    17101 cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa
    17161 acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta
    17221 aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat
    17281 aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg
    17341 gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag
    17401 cgccattttc agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct
    17461 ctcatgtcat cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg
    17521 agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct
    17581 cctgtccgtt aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt
    17641 tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat
    17701 cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg
    17761 tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt
    17821 ttgtggatga tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt
    17881 cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata
    17941 acggtaaata ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg
    18001 ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt
    18061 ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt
    18121 gctgattccc ttttgttaac ttatcaagtt cgtaggccag attgacattg atataatctt
    18181 tgtctttaac gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt
    18241 ccccgtgatt gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt
    18301 tcttttcttc taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca
    18361 ttggtgataa ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg
    18421 aactaccatc accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg
    18481 caggggcaac atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt
    18541 ccacgggcga tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta
    18601 agttcacttt tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg
    18661 gcaacggacc gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga
    18721 gatcgggtgc accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt
    18781 tgtcaaagtt tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat
    18841 cgttaaaatc tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg
    18901 tgccaccacc ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca
    18961 tataaatatt ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt
    19021 ccagcggcag gatgcattat cagaatgtat ttatatttat taaataataa aaaaagcccg
    19081 tgaatattca cgggctttat gtaatttaca ttgaattatt tttcctcgga cagatatttc
    19141 accgtatcag catactgctg aacaaaaaca tccatattgc tggtatccat accctgcgga
    19201 ttcagctgat atttaccgtt aacaaacatc gccggaacac cacgcaattg cacatcagct
    19261 gcggcttttt cctgctgagc gaccagagat ttcaccacga agctgttcca cgccgcgtcg
    19321 tactcttcac ctttaatacc tgcattgata aatacatcgc ggatatcaga tgctgaacga
    19381 atggtctggg ttttctgtac gccttcaaac agcggaacag tcactttgtc ttccacgccc
    19441 agcgccatcg ccacagccca tgcctgagtc agatctttgc ccaggtcacc acccatgaag
    19501 ttgacgtggt atttagtcat cttcacgcct tccggcagtt ttttcttcac attatcagaa
    19561 atatgcagaa cttcttcaaa ctgatagcag tgcgggcaga agaaagagaa aaactccagc
    19621 acttgcggcg cgccagctac cggtttttcc agggtagtgt actgtttacc atcttcatac
    19681 tgcgccgccg atgcgctaaa cgctaaaact aaaccagcca gcgccagcca aatctttttc
    19741 atgatctact ctctccgatt aatacatagg tgttaattgc aaagggggtt cttgtagaac
    19801 ttttgcctgt tctataaaag tcgccgtctg tcgtagccag taatcttccc cggttaacca
    19861 cgggaaattt ttcgggaacg cgggatcagc ccaatgccgc attagccagg caagataata
    19921 aaccaaacgc atggcgcgta aaggttcaat cagtccgatt tcagcggtgt cgaactcgct
    19981 aaattcttca taagcttcaa taatagtttc cagttgcatc cgctgctggg ctttatcgcc
    20041 attgagcaac atccacaaat cctgaatagc tggaccatta cgtgcatcat ccagatcaac
    20101 aaacattgga ccatcgcgcc agagaatatt cccggcgtgg cagtctccat gtagccgcag
    20161 aacggtgaaa tcttcccgcc agtgtgctgt aacggcggcg atcagctcat ctgtcgcttt
    20221 cagaaatgcc gctttcaacc cggaaggtat cagtgtagcg tcctcaaaca gcttgcgagg
    20281 ctcaatgaga tattcattca gaccgatggt cgggcgatga ataaaaagct gtttgcgccc
    20341 cgtctggtgc atacgcccta aataacgccc aaccgcttcc atctgatcga tattatcagc
    20401 ttcgaactgg cgaccaccga cgcttggaaa aacagcgaaa taaaatccct gatgattcaa
    20461 taaagtctga ccgttaaagg ccacaggtgc tgcgaccgga acttcatcat ttaccagctg
    20521 caacgcaaat tgatgttctt cgaggatttg atcggctgtc caacgttcag ggcgataaaa
    20581 tttgacgaca aaacgtcgac gctcttcgtc ctgaaattga tagacacggt tttcatagct
    20641 gttaagcggg gtaagaccgg aatccacccg gatcccttgc ttaaacagag cgtccatgat
    20701 ggtatccggg tgtagtgtct ggaaagtaaa agcgctgttg ttcatcccat catccggaaa
    20761 acattacgaa tgatgaagga tatcatttca cgatgatttt ggtggcgccg cttacaagct
    20821 tttactcttt aattacgccg cgagcgcgta acagcgccgt tttgaagtct tcttcataat
    20881 ctttctgcaa acccgggatc accgcatctt tcgcggaatc acgcattttc aggtgataga
    20941 tcagaatgtc atccgtcaaa tccgccagtt cgccgtcaaa acctgactct ttcgccagtt
    21001 tctgcaaaaa ttgcaacaag ttaaagtctg gctctttttg ccaggctggc tggaggagtt
    21061 caataacttc attcagacgt ttacatttca tagtaatgct ccttactctt gagacagaca
    21121 cgttagcagg gtcaatccca caataaaaga ggcgatatcg gtgaatctaa tgacgacgat
    21181 aacaggcgtt gtgctggcag gcggtaaagc cagacgaatg ggcggcgtag ataaaggatt
    21241 gctggactta aatggcaaac cgctgtggca acatgtcgct gacgcgctta agacgcagct
    21301 ctctcacgtc gtggttaatg ctaatcgtca tcaggaaatc tatcaggcag gcggtctgaa
    21361 agtgattgaa gattcactgg cggattaccc aggccctctg gcaggaatgc tttcagtaat
    21421 gcagcaggaa gctggtgagt ggtttttgtt ttgtccgtgc gatacgcctt acattcccca
    21481 tgatttagcc gcccggctta atcatcagcg caaagatgcg cctgtcgtgt gggtccatga
    21541 cggtgaacgc gatcacccga ctattgctct ggtaaaccgc gctattgagc ctttattact
    21601 ggaatatctg caagcaggag aacgccgggt aatggcattt atgcgtctgg ctggcggtca
    21661 tgcggttgat ttcagcgatc ataaagatgc atttgttaac gtgaatacgc cagaggagct
    21721 tgcccgatgg caggaaaaac gatgataccg ttactcgcct ttgccgcgtg gagtggtacc
    21781 ggaaaaacta cgcttttgaa aaaactgatc ccggcattat gcgccagagg gatccgtccg
    21841 gggctgatta agcatacgca ccatgatatg gatgttgata agccaggcaa agatagctat
    21901 gagctgcgca aggctggcgc ggtacaaacc atcgttgcca gccagcaacg atgggccttg
    21961 atgacggaaa caccagacga agaagagctg gatctacatt ttctcgcaag tcgaatggat
    22021 acctcaaagc tggatttgat tctggtcgaa gggtttaagc atgaagagat cgcaaagatt
    22081 gtgctgtttc gcgatggagc cggacatcga cctgaagaat tagtgataga caggcatgtt
    22141 attgctgtag ccagtgatgt gccgcttaat cttgatgtcg cattactgga tattaatgat
    22201 gttgaggggc tggctgattt tgtggtggag tggatgcaaa agcagaacgg ataagcaaga
    22261 gtaggctgga tcagacgc
//
LOCUS       DAZOYF010000055        22276 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZOYF010000055 DAZOYF010000000
VERSION     DAZOYF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.055"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(2..298)
                     /locus_tag="ACHUOF_001970"
     CDS             complement(2..298)
                     /locus_tag="ACHUOF_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR0611155.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="ACHUOF_001971"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="ACHUOF_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR0611156.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACHUOF_001972"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACHUOF_001972"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR0611157.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="ACHUOF_001973"
     CDS             complement(1784..2323)
                     /locus_tag="ACHUOF_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR0611158.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="ACHUOF_001974"
     CDS             complement(2353..3096)
                     /locus_tag="ACHUOF_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR0611159.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..3213)
                     /locus_tag="ACHUOF_001975"
                     /pseudo
     CDS             complement(3124..3213)
                     /locus_tag="ACHUOF_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3453..4091
                     /gene="ompW"
                     /locus_tag="ACHUOF_001976"
     CDS             3453..4091
                     /gene="ompW"
                     /locus_tag="ACHUOF_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR0611160.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4151..4657)
                     /locus_tag="ACHUOF_001977"
     CDS             complement(4151..4657)
                     /locus_tag="ACHUOF_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR0611161.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4703..5203)
                     /locus_tag="ACHUOF_001978"
     CDS             complement(4703..5203)
                     /locus_tag="ACHUOF_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR0611162.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5289..5468)
                     /locus_tag="ACHUOF_001979"
     CDS             complement(5289..5468)
                     /locus_tag="ACHUOF_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR0611163.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="ACHUOF_001980"
     CDS             complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="ACHUOF_001980"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR0611164.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="ACHUOF_001981"
     CDS             complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="ACHUOF_001981"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR0611165.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="ACHUOF_001982"
     CDS             complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="ACHUOF_001982"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR0611166.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="ACHUOF_001983"
     CDS             complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="ACHUOF_001983"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR0611167.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="ACHUOF_001984"
     CDS             complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="ACHUOF_001984"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR0611168.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(12400..12493)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="ACHUOF_001985"
     CDS             complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="ACHUOF_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR0611169.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12652..13533
                     /gene="rnm"
                     /locus_tag="ACHUOF_001986"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             12652..13533
                     /gene="rnm"
                     /locus_tag="ACHUOF_001986"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR0611170.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13530..14150
                     /locus_tag="ACHUOF_001987"
     CDS             13530..14150
                     /locus_tag="ACHUOF_001987"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR0611171.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            14251..15123
                     /gene="rluB"
                     /locus_tag="ACHUOF_001988"
     CDS             14251..15123
                     /gene="rluB"
                     /locus_tag="ACHUOF_001988"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR0611172.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="ACHUOF_001989"
     CDS             complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="ACHUOF_001989"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR0611173.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(15750..16508)
                     /locus_tag="ACHUOF_001990"
     CDS             complement(15750..16508)
                     /locus_tag="ACHUOF_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR0611174.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16728..17777
                     /gene="sohB"
                     /locus_tag="ACHUOF_001991"
     CDS             16728..17777
                     /gene="sohB"
                     /locus_tag="ACHUOF_001991"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR0611175.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17813..18064)
                     /locus_tag="ACHUOF_001992"
     CDS             complement(17813..18064)
                     /locus_tag="ACHUOF_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR0611176.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            18444..21041
                     /gene="topA"
                     /locus_tag="ACHUOF_001993"
     CDS             18444..21041
                     /gene="topA"
                     /locus_tag="ACHUOF_001993"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR0611177.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            21251..22225
                     /gene="cysB"
                     /locus_tag="ACHUOF_001994"
     CDS             21251..22225
                     /gene="cysB"
                     /locus_tag="ACHUOF_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR0611178.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         5603 a         5519 c         5718 g         5436 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccacac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgta accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaagc
     3241 gcggcaagtg gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa
     3301 tcacattaac caggattctc aatgcaactt ctaaattaat ccagatcaat aaatggtgaa
     3361 ttatcatatg taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt
     3421 cacgttttta taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg
     3481 cagtaacaac tcttctttct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc
     3541 gtgcaggttc tgcaaccgta cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg
     3601 gtggattcag cgtgaccaat aacactcaac tgggcttgac gtttacttat atggcgaccg
     3661 acaacattgg tgtggaatta ctggcagcga cgccgttccg ccataaaatc ggcacccggg
     3721 cgaccggcga tattgcaacc gttcaccatc tgccgccaac actgatggcg cagtggtatt
     3781 ttggtgatgc cagcagcaaa ttccgtcctt acgttggggc cggtattaac tacaccacct
     3841 tctttgataa tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga
     3901 aagattcctg gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt
     3961 tggttaacat gtcagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg
     4021 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
     4081 atcgttttta attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt
     4141 ataaagggta ttatttcttc gcttctacgc catcagtttc agaacgaatt aaaaatttct
     4201 cagttgtttg cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
     4261 tcgcttcgat gatcggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg
     4321 aggtgtaaca ggtgatttca aattgctcga agacatatcc gccaatagag cctttaacta
     4381 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg
     4441 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
     4501 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
     4561 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
     4621 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
     4681 actaaaagtt aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa
     4741 gggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa
     4801 gcttcgctgc tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
     4861 caatctcata atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt
     4921 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     4981 atttcatgcg cttaattttc agatttgatt ctgattccac aacttgatca attcgttcaa
     5041 tctgtccatg agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg
     5101 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     5161 aaagcagttg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     5221 tcactgcgag ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta
     5281 acggtgaatc aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc
     5341 gcttcagatg cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta
     5401 cggcctgcgt cggatgcctt ctcacggtct tcggcgaaat ttcctgaact accacgatgt
     5461 tcggccatgt tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc
     5521 gacactcatt aaaattagtc actaatgaga attagtcaaa ttaagcgcaa cgagaagata
     5581 gagggaaata tattttgagg aacattctgg atatattaac aattacctga ggaatatgtg
     5641 acttagagaa aattattgat tttactggtg ttatgttgcg ggattaattt gtttgattat
     5701 attactgttg ggcggaaaaa tgacgtaagt tgacgttcga ccggggtaag cgaaacggta
     5761 aaaagataaa tattaaatga atttaggatt tttccggctt cattaaagaa agttaaaatg
     5821 ccgccagcgg aactggcggc tgtgggatta actgcgcgtc gccgctttca tcggttgtac
     5881 aaaaactttc agtgccgcca gcattttctc tggctcatta atatgttgct cgatgatttt
     5941 aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg cttttacctg
     6001 atccggggcg gaaataccaa acccctgcaa tggaggtgca gcgttgtact ctttcagctt
     6061 cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca cgcccgctcg
     6121 tgacagcaaa tatgtgtaac cacgaccgta agaggctatc tggcgcagca ggtcgtcatc
     6181 ggcatttggc gggcagatga agataggtgc gacattatgg cgcaacgcgg cctggcggaa
     6241 gggcgcggac tcttcaactg gcacatcggc aaccagcacc gaatcgacgc cgactttctc
     6301 gcactgggca taaaactcat caatgccttt gttaaacacc agattggcat acatcaacag
     6361 gccaatggga atggtcgggt gtttctggcg aatcagtgcc agcatttcaa aacattgtgc
     6421 cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt tgaatggtcg ggccatccgc
     6481 cagtgggtcg gagaagggga tacctaactc cagcgcgtca gcaccggctt caattagcgt
     6541 atcgataatt ttcaatgact gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa
     6601 tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt ccatcagatt
     6661 tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa
     6721 aggttaacca ccagtagctg ctctttatcc gggttttcgc gcatcatttt cagcgcatgg
     6781 gccagggcgt gggaggattc cagcgccggg atgatccctt cgtgcaggca cagcgttttg
     6841 aaggcttcaa gggcttcatc atcggtaata gacacgtaat cagcgcgtcc agtgctgtta
     6901 agatacgcgt gttgtggacc gacagacggg aaatccagtc cggcggagat ggagtaagat
     6961 tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa ataaatgccc
     7021 acgcgaccat gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg accaccaggc
     7081 tccacaccaa tcaggccgac gttggtttca ttgatgaaat cagcaaacat gccgatggca
     7141 ttcgaaccgc cgccaacaca ggcgataacg gcatccggca ggcgaccttc tctttccaga
     7201 atctgcgctt tggtttcttc gccaatcatc cgctgaaact cacggacaat ggtcggataa
     7261 ggatgcgggc cagccgcggt gcccagcata tagtgcgcgg tttcataact gccggaccag
     7321 tcgcgtagcg cctcattaca ggcatctttc agcgtggcgg aaccgctatg taccgggatc
     7381 acttccgcac ccattaagcg catacgaaaa acgttaggcg actggcgttc aacgtctttg
     7441 gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc cgacgccacg
     7501 ccatgctgac cggccccggt ttcggcgatg atttcggttt tacccatccg cttcgccagc
     7561 aacgcctgcc ccagcacctg gttggtttta tgtgcgccgc cgtgcagcaa atcttcacgc
     7621 ttgagataca gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt cagtgcggtt
     7681 ggacgcccgg catagttttt cagcaggtcg ttgaactggg cttgaaattc aggatctttt
     7741 tgcgcgctga caaaagcttc ttccagctgg cgcagagcag gcatcaggat ttgtggcacg
     7801 tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt
     7861 aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg atgcccggtt
     7921 gcgactctac agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc
     7981 agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga ccatttaata
     8041 gtgaccagtc aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca tatttatcaa
     8101 cgtgctgaaa ctcgcgggcg ggcagggttt caccgacgct taatgctttc cagatggcaa
     8161 catgtgctgg cagagcttca cgcagcgtat cgatatacag ctgatcttca ttgccatgca
     8221 gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca atatcgtgat
     8281 tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa
     8341 cgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt
     8401 cataagctgc tttagcatct tgcccacgcg tcaggccaca tactttattc tcacccagca
     8461 acacccggcg cacggcggcg tgcaaatcgt catgggccat caacgccgaa ccaatcagaa
     8521 aaccgttagc gaagtggctt aactcgcgca cctgagcgta agtattgatg ccggattcgc
     8581 tgattaccgt tacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa
     8641 tcgacaaatc acgcagatcg cggttgttga tgccaacgac ctttgcccct aatgcaatgg
     8701 cgcgctccag ttcctcttca ttactgactt cggtcagcac ccccatctcc agactgtgag
     8761 cgacggctgc aagctggcga tattggtcgt catccagtac tgaaagcatt aataagcagg
     8821 catcggcctg gtaatagcgc gccagataga tctggtaagg gtcgataatg aagtctttac
     8881 ataaaatcgg ctgcggggcg atttggctga cgatgaggag gaaatcaaag ctcccctgaa
     8941 aatatttctc atcagtcagc accgaaattg ccgaagcgta atgtttataa atggcggcaa
     9001 tgcgtgccgg atcgaaatca tcacggatca cgccttttga cggcgacgct tttttacact
     9061 ccagaataaa cgccgtgcgt gcaccctgta gcgcatcata aaaatgtcgt gtgctcggct
     9121 gaacctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggtttct acccaaatcg
     9181 ccttgtctgc gacgattttc gctaaaacgg tttgcatcat ttaccctcgt gccgccagcg
     9241 cggtaactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt tgcgcattgg
     9301 cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacgttcgca gcgacggctg
     9361 cttcatgggc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca cggttttctt
     9421 caggtgttcc gcctgccagt tgctcctggt ggtagggagt caggccaaaa tcttcagcgg
     9481 tcaattgata gctcttaatt ttgccgtcat gcagctcggc aacgattgtc ggcgcgtgta
     9541 atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac cccagcacgc
     9601 gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt aacgccagcg
     9661 gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagggtgcgg gttttcagtt
     9721 gctggcgaac cggcatcgcg tggcggaatc cggtgtgata tttcggcgca aagaggaaac
     9781 atacgcctaa ctcgtccagc gcctggcgcg atttattggc gttcatatca agattaatac
     9841 cgaatgccgc cagcagatcc gacgagccgg atttactgga gacgctgcgg ttaccgtgtt
     9901 tcgccacttt cagcccacag gccgcggcga caaacgcact ggcggtagaa atattgatac
     9961 tgttgctgcc gtcaccgcca gtaccgacga tatcagcaaa cagataatcc gggcgcggga
    10021 acggcgctgc gttttccagt agcgcggttg ctgccccggc gatctcgttc gggtgctcac
    10081 cgcgaatttt catgctcacc agcgccgccg ccagttgttc cggcttcagc tcgccacgca
    10141 ccaccgctga aaacagctgg tggctttctt gttggctaag cgtctgcgcc tgatacagtt
    10201 tttccagaat cggttgcagc gtgttggctg gctctagttt ctgctgcgcc caggccagcg
    10261 tttgttccag caggcgagcg ccctgggtgg tgagaatgga ttccggatgg aactggaatc
    10321 cacaaacgcg atccgcatcg tgacgtactg ccatcaccat gccattaaaa tgggcgttga
    10381 tggttaaacc ggccggaatg ttactgccaa ccagcgagtg ataacgcgcc accggcagcg
    10441 ggtttgttaa tccggcaaac atcgcctgac cgtcatgttc aatgctcgac gctttaccgt
    10501 gaagaatttc gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt gcctgatgtc
    10561 cgaggcaaat gccaataatg ggcagcttgc cacgcaagcg ggtgaggagt tccggcatac
    10621 aaccggcttc gctcggcaca ccggggccag gagaaagcat cagtaccgga ttgctcatcg
    10681 tcgccaggcg ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc accacgttat
    10741 gaccattgct gcgcaactga tctgccaggt tgtacgtaaa agagtcgata ttatcgagca
    10801 gcagaatgtc agccatcaga aagtctcctg tgcatgatgc gcggtggcaa tagcgcgcag
    10861 tacagcgcgg gctttgttac gggtttcgtc ggcttccgac tgcggaacag aatcaaggac
    10921 tacaccagca cccgcttgca cggtggcgat accgttttcc accagcgccg agcggatcac
    10981 aatgcaggtg tcgagatcgc catgcgcggt gaaataacct accgcgccgc cgtagctgcc
    11041 gcggcgacga ccttccgcct cggcaattaa ctgcatagcg cgtactttcg gcgcaccgct
    11101 taacgtcccc atattcatac aggcgcgata agcgtgcagg gcgtcaagat cgtgacgcag
    11161 ttcgccgact acgcgagaga cgaggtgcat cacataggaa taacggtcaa ctttggtgag
    11221 atcggcgatg tagcggctgc caggggtgca aatgcgtgcc agatcattac gggcgagatc
    11281 aaccagcatc agatgttcag acagctcttt atggtcagta cgcatttcca gttcaatacg
    11341 gctgtcgaga tctctgtcca gtgaaccatc ggcgcgacga ccgcgtgggc gtgttccggc
    11401 aatcgggtag atctcaatct ggcggctggt ggcatcatac ttgagcgagc tttccggcga
    11461 cgcgccaaat agggtgaaat cattatcctg cataaaaaac atgtacgggc tgggattact
    11521 ctttttcagc acgtaatagg ccgccagcgg tgacgggcag ggcagagaga aacggcgaga
    11581 tggcaccacc tggaaaattt ctccagcgcg aatcgctttt tgcaacaaac gcactacgcc
    11641 accgaactct tcatcgctct gattacattc acaacgcata tgcggcacgg aaaccactgg
    11701 cagcggcggc gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag cagtgagacg
    11761 ttgtttttct tcttcattcg gagcaaacag gctggcctga atacgggtgc tttttttctg
    11821 atggtcaatc accatcagcg tttcagcgag ataaaaacag aaatcagggc agttattttc
    11881 cgctgacagt tgcggtaaat cttcaaatcc cgccacaagg tcataagaga acaggccgcc
    11941 gaagaacatt gcttctcgtt cttccttcgg tacattcaac agattctgca ataaacggaa
    12001 agcgtcaaaa accgaaaggg agcataagcg ggcgtcttca tccagcagtg gactgacagg
    12061 ggggaagcgc agcacacggc agtttggtga ttgttcactt tccacacccg caggcagggc
    12121 gttatccagt agtgccagca gggcttcgcc attgccggaa agtgcctgaa ttgtgacggt
    12181 gtcacctaat gctgtaatgc gcagcgcact gtctatcagc agcaggcttt ttaaatcatc
    12241 tttgctgtcg atatctgcgg attccagcag cagcgttgcc ggacgattcc cacacaactg
    12301 gtgaaaaagc gcagtcgggt tgtcgcgata agcgccttcg caggttagca gttcgagagt
    12361 cggtttttgt gtttgcattg ttattctcta attttgttca aaaaaaagcc cgctcattag
    12421 gcgggctggg tatctgattg ctttacgcat ggtgaataca ctgcccgttt caggaagtgc
    12481 gccaccaacc tttcagtacg aaaattgctt tcattgtcga tacccttttt acgtgaactt
    12541 gcgtactagt taactggttc gatgattaat tgtcaacagc tcatttcaga atatttgcca
    12601 gacccgttat gatgtcggcg caaaaaacat tatccagaac gggagtgcgc cttgagcgac
    12661 acgaattatg cagtgattta cgacctgcac agccatacca cagcttccga tggctgcctg
    12721 acgccagaag cattggtgca ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc
    12781 gaccatgaca ccacagctgc aatcgcgcca gcaagagagg aaatttcacg ttcgggactg
    12841 gcgctgaatc ttattcccgg cgtggaaatt tccacggtct gggaaaatca tgaaattcat
    12901 attgtcgggc tgaatattga tattactcat ccgctaatgt gtgagttcct cgcgcagcag
    12961 acagaacggc gcaatcagcg ggcgcagcaa attgccgaaa gacttgaaaa agcgcaaata
    13021 cctggggcgc tggaaggcgc acaacgactg gcgcagggcg gcgcagtgac gcgcggtcat
    13081 tttgcgcgtt tccttgtgga gtgcggcaaa gccagttcaa tggcagatgt ctttaaaaag
    13141 tatctggcgc gcgggaaaac cggatacgtt ccgccacagt ggtgtacaat agaacaagct
    13201 attgatgtca ttcatcattc tggcggtaag gcggtgctag ctcatcctgg gcggtacaat
    13261 cttagtgcta aatggctgaa aagattggtt gcgcattttg ccgaacacca cggcgacgcg
    13321 atggaagtcg cgcagtgcca gcaatcgccc aatgaacgca cccagctggc cacacttgcg
    13381 cgtcagcatc atttatgggc atcacaagga tctgattttc atcagccatg cccgtggatc
    13441 gaactgggtc gtaaactctg gctgcccgca ggcgttgaag gcgtctggca gctatgggaa
    13501 cagccgcaga acaccacaga gagggaatta tgagccagtt tttttatatt catcctgata
    13561 acccgcagca acgtctgatc aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg
    13621 tctatccaac tgattccggc tatgcgctcg gctgtaaaat tgaagataaa aacgccatgg
    13681 agcgtatttg tcgtattcgc cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg
    13741 atctttctga actctcgacc tattcattcg tggataacgt tgcgtttcgt ttgatgaaaa
    13801 acaacacgcc gggcaactat accttcatcc tgaaggggac gaaggaagtg ccacgccgtc
    13861 tgttgcagga gaaacgcaaa accatcggta tgcgtgtgcc ttctaacccg attgcgcagg
    13921 cgttgcttga agcactgggc gaaccgatgc tttccacttc gctaatgctg ccaggcagcg
    13981 aatttaccga atccgatccc gaagaaatta aagaccgtct ggaaaaacag gtagatttga
    14041 ttattcacgg cggctatctc gggcagaaac caacaacggt tattgatctt accgatgata
    14101 cgccggtcgt ggtgcgtgaa ggcgtaggtg atgtgaagcc tttcttataa gtgcgtaact
    14161 ctgtatacta cgcggcctta aaatggcgct atacggcgcc attgattctc ctgttcgacg
    14221 cctgtgaagg cgacaccaaa aggaagctct atgagcgaaa aattacagaa agtgctggcc
    14281 cgcgctggcc acggctcccg ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt
    14341 gttgatggca aaattgctaa actcggcgat cgcgttgaag ttacccctgg gctgaaaatt
    14401 cgtatcgatg gtcatctgat ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg
    14461 gcctattaca aaccggaagg tgagttgtgt acccgtaacg atccggaagg gcgtccgacg
    14521 gtgtttgacc gtctgccaaa gctgcgtggc gcacgctgga ttgccgtagg tcgtctggac
    14581 gttaacacct gtggtctgct gctgttcacc accgatggtg aactggcaaa ccgtttaatg
    14641 catccaagcc gtgaagttga acgtgaatac gccgtgcgtg tatttggtca ggttgacgac
    14701 gcgaaactgc gtgatttgag tcgtggcgtg caactggaag atggtccggc agcttttaaa
    14761 accatcaagt tcagcggcgg cgaagggatc aaccagtggt acaacgtgac tctgaccgaa
    14821 ggccgtaacc gtgaagttcg tcgtctgtgg gaagcggttg gtgtgcaggt gagccgcctg
    14881 atccgtgttc gttacggtga tatcccactg ccaaaaggtc tgccacgcgg tggctggacg
    14941 gagctggacc tcgcccagac taactatctg cgcgaactgg tggagcttcc gccagaaacc
    15001 agctctaaag tcgctgtaga aaaagaccgt cgtcgcatga aggctaatca gattcgtcgt
    15061 gcggtgaaac gtcacagtca ggtgagcggc agtcgtcgtt ctggcgggcg taataacggt
    15121 taatcaaagt atgccggacg tcatatccgg catttttaca gattaataat caatccctat
    15181 ttgcgcctta acaccggcat caaacgcatg tctgatgggg cgcagctcac ttaccgtatc
    15241 cgccagttca agaatatccc gatgacagcc tcgacccgtg ataatcaccg tctgttgatg
    15301 tggacgttca tttaacgcct gcaccacttc ttccagtggc aaatagtcat acgccaccat
    15361 atacgtcagt tcatcaagca aaaccatatc cagtgaggaa tcagcaagca tccgctttgc
    15421 atgttgccag acttcgcggc aggcggcggt atcagactcg cggttttgtg tatcccaggt
    15481 aaagcccgtt gccatcacct gaaactcaac gccatgtggc tccagcagat tgcgttcgcc
    15541 attaggccag gtgcctttaa taaactgcac gacgcccact ttttttccgt gaccaactgc
    15601 gcgtgttgcc gtaccaaatg ccgcggtggt tttgcctttt ccattgccgg taaagacgat
    15661 gataataccg cgttcatcct gggcctgggc cacacgagca tctacttttt ctttcactcg
    15721 ctgctgacgc tgttggtagc gttcatcact cattgggaaa ttcctggttt acggcccggt
    15781 tgggcgtcaa aggtcatgcc ggttttacgg cggctgtcat cgcccatcag ccagaggtag
    15841 agcggcatga tatcagcggg tgttttaagt ttctgtggat cttcggtcgg gaaggcgctt
    15901 gcgcgcattg cggtgcgcgt accgcctggg ttaatgcagt tgacgcgcag gcgctgctga
    15961 tattcatcgg ccagtacctg catcatccct tcggtggcaa atttcgacgc tgcatatgca
    16021 ccccagttgg ctcgtccctg acgtccaacg cttgatgaag taaagaccag tgaaccggcg
    16081 tccgatttga gtaataaagg aagcagtgcc tgggtgagca taaaggtggc attaacgttg
    16141 atctgcatga cgtcctgcca gacctgcgga ttttgttcgc tcattgggca aacatcgccg
    16201 agcaatccgg cattatgcaa aacaccatcc agacgcggat aattaacgac aatgcgctgt
    16261 gccagttgtt ggcaattttc ggacgtgcag gtcagcaaat cgagaataaa ccactgtggc
    16321 tgacgcccag tttcttcgtt tatgtggctg gctacctgac gtaatttttc ttcattacgg
    16381 cccaacagaa tcactgtcgc accatagcgt gcatacgtca tcgcggcttc acgaccaatc
    16441 ccatcgctgg ctcccgtcac caggataatg cgatcattga gtaaatcttg ttttggctgg
    16501 taatgcatgg ctactcctca acgacgttgt ctgtcgcgct gccagtgtac tttatgactt
    16561 tggggcttta tgcctgaaac agaagggcat ttcaatcagc ctggggcgat aacaccgcaa
    16621 aatggatact ttgtcatact ttcgctgcaa taacatctct gcgagacggc ttaacatgcc
    16681 tgttgtaaac tgtgagccaa agcgttgttt aaccaaggtg gggactcgtg gaattgttgt
    16741 ctgaatatgg tttgtttttg gctaaaatcg ttaccgttgt gctagcgatt gcggcgattg
    16801 ccgccattat tgtcaatgtt gctcaacgta ataaacgcca gcgtggcgag ttacgggtca
    16861 acaatctcag cgaacagtat aaggagatga aagaagaact ggccgcggcg ctgatggact
    16921 cacatcagca aaaacagtgg cacaaagcgc agaagaaaaa gcacaagcaa gaagcgaaag
    16981 cagcaaaagc gaaagccaaa ctgggcgagg tggcaactga cagtaaacct cgcgtctggg
    17041 tgctggattt taaaggcagc atggacgccc atgaagtgaa ctcgctacgt gaagagataa
    17101 cggcggtact cgcagcattc aaaccgcagg atcaggttgt gctccgtctg gaaagccctg
    17161 gtggcatggt gcatggttac gggttggcgg cttcgcagct gcagcgtctg cgtgataaaa
    17221 acattccttt aactgttacg gtagacaaag tcgctgccag cggcggttac atgatggcct
    17281 gtgtggcgga caaaattgtt tccgcaccgt ttgctattgt gggttccatt ggggtggtgg
    17341 cgcaaatgcc caactttaac cgcttcctga aaagcaaaga tattgatatc gaactgcaca
    17401 ccgccgggca gtataagcgc acgctgacct tgctgggtga aaataccgaa gaagggcggg
    17461 agaaattccg cgaagagctg aacgaaacgc atcagttatt taaagatttt gtgaagcgta
    17521 tgcgtccgtc tctggatatt gaacaggtgg caacgggtga acactggtac ggacaacagg
    17581 cggtagagaa aggcctggtt gatgaaatca acaccagtga tgaagttatt cttagcctga
    17641 tggaaggccg tgaagtggtt aatgtacgct atatgcagcg taaacgactc attgaccgat
    17701 tcaccggcag cgcggcagag agcgccgatc gattgttgct acgctggtgg cagcggggtc
    17761 aaaagccatt gatgtaaaag acaaacgcga ggctaagacc tcgcgttttg ctttaatcaa
    17821 ccagatgata tttttctgaa agcacatggg ccaggtgttt gaacatatta aacaccgcgg
    17881 tgcttttggc tgttggcaat ccttgttcat ctaaaaagta gtcgccggta aataccagca
    17941 cgccattacg ctgcgtgacg ccggtggctt caatccctgc gagcgtatcc tcatgctcac
    18001 gaatgatttt gttggcctct ttcaacagcg tttcgcggtc gatgggttgt gtctctttgt
    18061 tcattattta ctccttaaac aaggacatta gtctacgcca ggcatggctt gcagacaaat
    18121 ataccacgct ggtggcaaga gcgccttact ggcaactttg gattttgcat gctaataaag
    18181 ttgcgtatcg gattttatca ggtacagtgt gacgctttcg tcaatctggc aatagatttg
    18241 cttgacattc gaccaaaaat ccgtcgtgct atagcgcctg taggccaaga cctgttaact
    18301 cagtcacctg aattttcgtg aacagagtca cgacaagggg ttgatatccg cagagagcga
    18361 gtccatatcg gtaactcgtt gccagtggaa ggtttatcaa cgtgcgacgc attcctggaa
    18421 gaatcaaatt aggtaaggtg aatatgggta aagctcttgt catcgttgag tccccggcaa
    18481 aagccaaaac gatcaacaag tatctgggtt gtgactacgt ggtgaaatcc agcgtcggtc
    18541 acatccgcga tttgccgacc agtggctcag ctgccaaaaa gagtgccgac tctacctcca
    18601 ccaagacggc taaaaagcct aaaaaggatg aacgtggcgc tctcgtcaac cgtatggggg
    18661 ttgacccgtg gcacaattgg gaggcgcact atgaagtgtt gcctggtaaa gagaaggtcg
    18721 tctctgaact gaaacaactg gctgaaaaag ccgaccacat ctatctcgca accgaccttg
    18781 accgcgaagg ggaagccatt gcatggcacc tgcgggaagt gattggtggt gatgatgcgc
    18841 gctatagccg agtggtgttt aacgaaatta ctaaaaacgc gatccgccag gcatttaaca
    18901 aaccgggtga gctgaatatt gatcgtgtta atgcccagca ggcgcgtcgc tttatggacc
    18961 gcgtggtggg gtatatggtt tcgccgctgc tgtggaaaaa gatcgctcgt ggtctgtctg
    19021 ccggtcgtgt gcagtcggtg gcggtccgcc tggtggtaga gcgtgagcgt gaaattaaag
    19081 cgttcgtacc ggaagagttc tgggaagtcg atgccagcac gactacgcca tctggtgaag
    19141 cgttggagtt gcaggtgact catcagaatg acaaaccgtt ccgtccggtc aacaaagaac
    19201 aaactcaggc tgcggtaagt ctgctggaaa aagcgcgcta cagcgtgctg gaacgtgaag
    19261 acaaaccgac aaccagtaaa cctggcgctc cttttattac ctctacgctg caacaagctg
    19321 ccagcacccg tcttggtttt ggcgtgaaaa aaaccatgat gatggcgcag cgtttgtatg
    19381 aagcaggcta tatcacttac atgcgtaccg actccactaa cctgagtcag gacgcggtaa
    19441 atatggttcg cggttatatc agcgataatt ttggtaagaa atatctgccg gaaagtccga
    19501 atcagtacgc cagcaaagaa aactcacagg aagcgcacga agcgattcgt ccttctgacg
    19561 tcaatgtgat ggcggaatcg ctgaaggata tggaagcaga tgcgcagaaa ctgtaccagt
    19621 taatctggcg tcagttcgtt gcctgccaga tgaccccagc gaaatatgac tccacgacgc
    19681 tgaccgttgg tgcgggcgat ttccgcctga aagcacgcgg tcgtattttg cgctttgatg
    19741 gctggacaaa agtgatgcct gcactgcgta aaggcgatga agatcgtatc ttaccggcag
    19801 tcgataaagg cgatgctctg acgctcgttg aactaacacc agcccagcac tttaccaagc
    19861 cgccagcccg tttcagtgaa gcatcgctgg ttaaagaact ggaaaaacgt ggtatcggtc
    19921 gtccgtctac ctatgcgtcg atcatttcga ccattcagga tcgtggctat gtgcgagtag
    19981 aaaatcgtcg tttctatgcg gaaaaaatgg gcgaaatcgt caccgatcgc cttgaagaaa
    20041 atttccgcga gttaatgaac tacgacttta ccgcgcagat ggaaaacagc ctcgaccagg
    20101 tggcaaatca cgaagcagag tggaaagctg tactggatca cttcttctcg gatttcaccc
    20161 agcagttaga taaagctgaa aaagatccgg aagagggtgg tatgcgcccg aaccagatgg
    20221 ttctgaccag cattgactgc ccgacttgtg gtcgcaaaat ggggattcgc acagcgagta
    20281 ccggggtatt ccttggctgt tctggctatg cgctgccgcc gaaagagcgt tgcaaaacca
    20341 ccattaacct ggtgccggaa aacgaagtgc tgaacgtgct ggaaggcgaa gatgctgaaa
    20401 ccaacgcgct gcgcgcaaaa cgtcgttgcc cgaaatgcgg cacggcgatg gacagctatc
    20461 tcatcgatcc gaaacgtaag ttgcatgtct gtggtaataa cccaacctgc gacggttacg
    20521 agatcgaaga gggcgaattc cgcattaaag gttatgacgg cccgatcgtt gagtgtgaaa
    20581 aatgtggctc tgaaatgcac ctgaaaatgg ggcgtttcgg taaatacatg gcctgcacca
    20641 acgaagagtg taaaaacaca cgtaagattt tacgtaacgg tgaagtggca ccaccgaaag
    20701 aagatccggt gccattacct gagctgccgt gcgaaaaatc agatgcttat ttcgtgctgc
    20761 gtgacggtgc tgccggtgtg ttcctggctg ccaacacttt cccgaaatcg cgtgaaacgc
    20821 gtgcgccact ggtggaagag ctttatcgct tccgcgaccg tctgccggaa aaactgcgtt
    20881 atctggccga tgcgccacag caggatccgg aaggtaataa gaccatggtt cgctttagcc
    20941 gtaaaaccaa acagcaatat gtctcttcgg aaaaagacgg aaaggcgact ggctggtcag
    21001 cattttatgt tgatggcaaa tgggttgaag gaaaaaaata acctttaatt ctgtcaggtt
    21061 tttataaaca aagggtcgcg aaagcggccc ttttttattg catattattt tttcttcaca
    21121 cctatacact aaggctataa atgatatagt ggttatagtt agcacctttt ttattattaa
    21181 atcgtattag tcacccgcca ggtgtgacga aaaaacgatg ttctgatggc gtctaagtgg
    21241 atggtttaac atgaaattac aacaacttcg ctatattgtt gaggtggtca atcataacct
    21301 gaatgtctca tcaacagcgg aaggacttta cacatcacaa cccgggatca gtaaacaagt
    21361 cagaatgctg gaagacgagc taggcattca aattttttcc cgaagcggca agcacctgac
    21421 gcaggtaacg ccagcaggac aagaaataat tcgtatcgct cgcgaagtcc tgtcgaaagt
    21481 cgatgccata aaatcggttg ccggtgagca cacctggccg gataaaggct cgctgtatat
    21541 cgccaccacg catacccagg cacgctacgc gttaccaaac gttatcaaag gctttattga
    21601 gcgttatccc cgcgtttctt tgcatatgca ccagggcccg ccgacacaaa ttgctgatgc
    21661 cgtctctaaa ggcaatgccg atttcgctat cgccacagaa gcgctgcatc tgtatgaaga
    21721 tttagtgatg ttaccgtgct accactggaa tcgggctatt gtagtcactc cggatcaccc
    21781 gctggcaggc aaaaaagcca ttaccattga agaactggcg caatatccgt tggtgacata
    21841 taccttcggc tttaccggac gttcagaact ggatactgcc tttaaccgcg cagggttaac
    21901 gccgcgtatc gttttcacgg caacggatgc tgacgtcatt aaaacttacg tccggttagg
    21961 gctgggggta ggggtcattg ccagcatggc ggtggatccg gtcgccgatc ccgaccttgt
    22021 gcgcgttgat gctcacgata tcttcagcca cagtacaacc aaaattggtt ttcgccgtag
    22081 tacttttttg cgcagttata tgtatgattt cattcagcgt tttgcaccgc atttaacgcg
    22141 tgatgtcgtt gatgcggctg tcgcattgcg ctccaatgaa gaaattgagg tcatgtttaa
    22201 agatataaaa ctgccggaaa aataatttct tgcgttattt tcggcacctt ttatgtagcg
    22261 aaggtgccgg aatata
//
LOCUS       DAZOYF010000056        22109 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZOYF010000056 DAZOYF010000000
VERSION     DAZOYF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.056"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1044)
                     /locus_tag="ACHUOF_001995"
     CDS             complement(<1..1044)
                     /locus_tag="ACHUOF_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR0611179.1"
                     /translation="MKLRGGTWHCDFVAPDGSRVRRSLETSDKRQAQELHDRLKAEAW
                     RVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLHFREAILSDITTEKIM
                     EAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKTRHLAILRAILNMAVE
                     WGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVVMFAITTGLRRSNVTD
                     LEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQGLHKRWVFVHTKPAYR
                     SDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWASWLVQSGVSLLALKEM
                     GGWETLEMVQRYAH"
     gene            complement(927..1274)
                     /locus_tag="ACHUOF_001996"
     CDS             complement(927..1274)
                     /locus_tag="ACHUOF_001996"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0611180.1"
                     /translation="MEEEIFTREEAASYLKVDKGTITQWIRSGRLQAAKINPDKPKSP
                     YRICKSDCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLK
                     HRTKGKRKNFTIV"
     gene            complement(1361..1705)
                     /locus_tag="ACHUOF_001997"
     CDS             complement(1361..1705)
                     /locus_tag="ACHUOF_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611181.1"
                     /translation="MSDLAMKVLKWQSTDDVGISSATLASIACGLKKNIYGHSFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            complement(1806..1985)
                     /locus_tag="ACHUOF_001998"
     CDS             complement(1806..1985)
                     /locus_tag="ACHUOF_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eag protein"
                     /protein_id="HFR0611182.1"
                     /translation="MSCPKCGSGNIAKEKTMRGWSGDYVCCDCGYNDSKDAFGERGKN
                     EFVKINKEREGNEKS"
     gene            complement(2082..3011)
                     /locus_tag="ACHUOF_001999"
     CDS             complement(2082..3011)
                     /locus_tag="ACHUOF_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0611183.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVAY
                     IIQDSDARSRGEKGILRYFANISDEDINEYEITVTPLYAAPPETVSVPDAMEMDDDFD
                     SAFEHGKAVGWNAYRAAMLQSGNFRENKNSSTNNFREIAETSTNYPVIPSEVLSAIQK
                     VAKIRADFDDFDGDRRGIGDCLDEAEQELIVTINKYASQLAAEPIAPNDVREQTAIPQ
                     VPVTPDGWISCSERMPVIGELNWRTSFPLLITCEIGVIPAYYGFVSVNGNKHYGFMES
                     LKYGDDNGNHPQTNEYGLISNVTHWMPLPEPPL"
     gene            complement(3014..3205)
                     /locus_tag="ACHUOF_002000"
     CDS             complement(3014..3205)
                     /locus_tag="ACHUOF_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611184.1"
                     /translation="MDGQISIVRPGACDDREIRLIIRLAMGKTITALITPENLALALT
                     GKSDLPVELKLRNIEIKVK"
     gene            complement(3207..3836)
                     /locus_tag="ACHUOF_002001"
     CDS             complement(3207..3836)
                     /locus_tag="ACHUOF_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFR0611185.1"
                     /translation="MSEINYQALREAAERAIPAMERLLMLPADDLLSEQELKDYGVDI
                     DALNAFKFLAGPETVLALLDERVRNQQYIKSRDQENEDIALTVGKLLIENGRLVADTL
                     RHLADNEIDSDYFAITSTNENGTEIDHEMAITDYALQAAGTVDELVAALESAENRIAE
                     LGERGILLPERSSMLHRTDFHDDYQTVMAYKVSEVIAAIRAAGIRIKGE"
     gene            complement(3833..4408)
                     /locus_tag="ACHUOF_002002"
     CDS             complement(3833..4408)
                     /locus_tag="ACHUOF_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hNH endonuclease"
                     /protein_id="HFR0611186.1"
                     /translation="MKQMSLIEMDGFLKGKCIPCDLKVNETNAEYLVRKFAEAEAKIS
                     ALYEDHQKAIESIKQADAAVKLAHEKFSALAAENAGMKSVIEYCINPDNQPEYHDQGM
                     GCGVEDHGYQRDGYSACYYGWESAMDRVYSEVIPDAIPETPATDTFLDEVRAQGVEMF
                     AASMKVVGGHEHPYSAMANEFAAQLRKGGGQ"
     gene            complement(4405..4569)
                     /locus_tag="ACHUOF_002003"
     CDS             complement(4405..4569)
                     /locus_tag="ACHUOF_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFR0611187.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKY
                     LNAMWRSGKK"
     gene            complement(4580..4873)
                     /locus_tag="ACHUOF_002004"
     CDS             complement(4580..4873)
                     /locus_tag="ACHUOF_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFR0611188.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEELERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(4897..5280)
                     /locus_tag="ACHUOF_002005"
     CDS             complement(4897..5280)
                     /locus_tag="ACHUOF_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611189.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     TSGNSQQQQSSNHQNHPEYDDSIPF"
     gene            complement(5280..5885)
                     /locus_tag="ACHUOF_002006"
     CDS             complement(5280..5885)
                     /locus_tag="ACHUOF_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFR0611190.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAALQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDIYNIRRDELEGAAA"
     gene            complement(5987..6145)
                     /locus_tag="ACHUOF_002007"
     CDS             complement(5987..6145)
                     /locus_tag="ACHUOF_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611191.1"
                     /translation="MSFTDNWSDEEFIRQMKELIGNEGDIHVTCNHSEGEQVTETHVH
                     AESSLVSP"
     gene            complement(6142..6294)
                     /gene="kil"
                     /locus_tag="ACHUOF_002008"
     CDS             complement(6142..6294)
                     /gene="kil"
                     /locus_tag="ACHUOF_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFR0611192.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINI
                     PWYRRK"
     gene            complement(6279..6413)
                     /locus_tag="ACHUOF_002009"
     CDS             complement(6279..6413)
                     /locus_tag="ACHUOF_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611193.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLISAAKKQLEALCAMK
                     "
     gene            complement(6608..7078)
                     /locus_tag="ACHUOF_002010"
     CDS             complement(6608..7078)
                     /locus_tag="ACHUOF_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611194.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQKRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            complement(7087..7413)
                     /locus_tag="ACHUOF_002011"
     CDS             complement(7087..7413)
                     /locus_tag="ACHUOF_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFR0611195.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPRSEMGVTCVGRQKMKL
                     GSKPLI"
     gene            complement(7894..8088)
                     /locus_tag="ACHUOF_002012"
     CDS             complement(7894..8088)
                     /locus_tag="ACHUOF_002012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611196.1"
                     /translation="MNGDRYEFTKDSTEDSVFHVTINGDKSSVYESVSGVYPEMKYTA
                     LSSNTMVGEYQSGGGITVET"
     gene            complement(8178..8810)
                     /locus_tag="ACHUOF_002013"
     CDS             complement(8178..8810)
                     /locus_tag="ACHUOF_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFR0611197.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            8914..9129
                     /locus_tag="ACHUOF_002014"
     CDS             8914..9129
                     /locus_tag="ACHUOF_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0611198.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            9246..9527
                     /locus_tag="ACHUOF_002015"
     CDS             9246..9527
                     /locus_tag="ACHUOF_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015995142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFR0611199.1"
                     /translation="MGTIATKSKKAARIESALLNKLALMGQKTFARAMGVPEYQVSRW
                     KNGFFSQVSMMLAVLEYGIEDDEMAELTKRLADYLTKEKAPKNGEFFEA"
     gene            9562..9723
                     /locus_tag="ACHUOF_002016"
     CDS             9562..9723
                     /locus_tag="ACHUOF_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611200.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            9710..10540
                     /locus_tag="ACHUOF_002017"
     CDS             9710..10540
                     /locus_tag="ACHUOF_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFR0611201.1"
                     /translation="MGVVKLADYRPQLEVVEHRVAELEDGYTRTANTLLEAAMLSGLT
                     LHQLLIVMAVWRKTYGYNKKIDWIGNEQFAELTGMAPTKCSTAKNELIRMGVLIQVGR
                     QVGMNTNISEWKTKVNGFGKTFTNSVKQTFTKSVKSNLPNQSNTKDNIQKTINTNTPL
                     PPNGGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQL
                     KTPNVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA
                     "
     gene            10537..11913
                     /locus_tag="ACHUOF_002018"
     CDS             10537..11913
                     /locus_tag="ACHUOF_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0611202.1"
                     /translation="MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIDELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            11986..12312
                     /locus_tag="ACHUOF_002019"
     CDS             11986..12312
                     /locus_tag="ACHUOF_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611203.1"
                     /translation="MADWQIPIIILAGASLVAGFILLKKHKDRDQKVEVLYGYPANST
                     TWLTIYHYRKSGRWVFEWDDLFAEKRPKSWGDISECMMFEERKSGATREEFNEAWARL
                     SERGYL"
     gene            12309..12509
                     /locus_tag="ACHUOF_002020"
     CDS             12309..12509
                     /locus_tag="ACHUOF_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611204.1"
                     /translation="MSKYEKLDQNILSMLSERPTPVFDIWLKWRSNGMYIETIDRRMQ
                     YLRKKGLVANVRGKGWVKINLS"
     gene            12521..12709
                     /locus_tag="ACHUOF_002021"
     CDS             12521..12709
                     /locus_tag="ACHUOF_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004904153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611205.1"
                     /translation="MDDSLEIFEEWCADELGVSAYFIRQMRSKNILGVIEYKRVEINK
                     RYRAWMAAVRAAGVKVKE"
     gene            12712..12876
                     /locus_tag="ACHUOF_002022"
     CDS             12712..12876
                     /locus_tag="ACHUOF_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611206.1"
                     /translation="MSAYEEIMLALRFFFGVEEDENVNEIIGQDHDPIGTIAAALDDY
                     RSVNGEETNV"
     gene            12857..13297
                     /locus_tag="ACHUOF_002023"
     CDS             12857..13297
                     /locus_tag="ACHUOF_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFR0611207.1"
                     /translation="MRKLTFELRSPIHQQNAIQAIQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGRSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDKTE"
     gene            13294..13884
                     /locus_tag="ACHUOF_002024"
     CDS             13294..13884
                     /locus_tag="ACHUOF_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFR0611208.1"
                     /translation="MNKYRLIYADPPWQYRDKANDGNRGAGHKYDVMNVQDICRLPVW
                     DLADPESCLLAMWWVPTQPAEALKVIEAWGFRLMTMKGFTWHKTNKHKGNSAIGMGHM
                     TRANSEDCLFAVRGRLPERMDASICQHFTAPRMEHSAKPPIVRDMLAKLLGDVPRCEL
                     FSRDKVTGWDMWGNQCDSDFELAPGMAIKPCKMVIA"
     gene            13881..14054
                     /gene="ninD"
                     /locus_tag="ACHUOF_002025"
     CDS             13881..14054
                     /gene="ninD"
                     /locus_tag="ACHUOF_002025"
                     /inference="COORDINATES: protein motif:HMM:NF036649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinD"
                     /protein_id="HFR0611209.1"
                     /translation="MKHCYRCGERKEDDRFRPGQPYWNRWCLRCERTPTGVLPLPQEK
                     EDVWRDSDEVSPT"
     gene            14021..14196
                     /locus_tag="ACHUOF_002026"
                     /pseudo
     CDS             14021..14196
                     /locus_tag="ACHUOF_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
     gene            14199..14600
                     /locus_tag="ACHUOF_002027"
     CDS             14199..14600
                     /locus_tag="ACHUOF_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611210.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMMSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVENHHAVRQAKRKANMTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            14560..14790
                     /locus_tag="ACHUOF_002028"
     CDS             14560..14790
                     /locus_tag="ACHUOF_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFR0611211.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDGQTSAKTM"
     gene            14762..15373
                     /locus_tag="ACHUOF_002029"
     CDS             14762..15373
                     /locus_tag="ACHUOF_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFR0611212.1"
                     /translation="MAKPARRRCKNEECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIG
                     QEAVDEIESNHNRHRWTVVECKAIKAEYQQKLKDLRNSRSEAA"
     gene            15370..15576
                     /locus_tag="ACHUOF_002030"
     CDS             15370..15576
                     /locus_tag="ACHUOF_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFR0611213.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            15554..16219
                     /locus_tag="ACHUOF_002031"
     CDS             15554..16219
                     /locus_tag="ACHUOF_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR0611214.1"
                     /translation="MRYYEKIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NIDYDKEILAKALSHKADELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            16216..16839
                     /locus_tag="ACHUOF_002032"
     CDS             16216..16839
                     /locus_tag="ACHUOF_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0611215.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            17272..17595
                     /locus_tag="ACHUOF_002033"
     CDS             17272..17595
                     /locus_tag="ACHUOF_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFR0611216.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            17579..18055
                     /locus_tag="ACHUOF_002034"
     CDS             17579..18055
                     /locus_tag="ACHUOF_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFR0611217.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            18052..18489
                     /locus_tag="ACHUOF_002035"
     CDS             18052..18489
                     /locus_tag="ACHUOF_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0611218.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKAASTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            18694..19236
                     /locus_tag="ACHUOF_002036"
     CDS             18694..19236
                     /locus_tag="ACHUOF_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0611219.1"
                     /translation="MNYPTVVNDIDFRDLIFVANNDPVTDSFMVAKAFGKLPKNVVRD
                     IERTIEACPPEFDTKLNFELCYKNNELQNGKPQKFYRLRKDGLMLLVMSDTKKEAMRI
                     KIAYINAFNWMYAMLQVGHRQFEEERNAVMLEYMKEKDVASMSGRLLNRWGKIKKPQL
                     MARIERLEQHGQTVIPGLIN"
     gene            19456..19671
                     /locus_tag="ACHUOF_002037"
     CDS             19456..19671
                     /locus_tag="ACHUOF_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0611220.1"
                     /translation="MDDNGNQWVNVEHRLPESKEGMWSKEVIALTDTGDVFKLSCMGS
                     YWQRTKAFIDSGASKVTHWMPLNYPDD"
     gene            19819..20061
                     /locus_tag="ACHUOF_002038"
     CDS             19819..20061
                     /locus_tag="ACHUOF_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFR0611221.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITIRTFEAVRKSKEALDLFTTGA"
     gene            20097..20585
                     /locus_tag="ACHUOF_002039"
     CDS             20097..20585
                     /locus_tag="ACHUOF_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR0611222.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWADDNPL
                     WEGKVFSYQGEIIKANVPKMRAMTISGLCTFLDITRQTWGTFRSMEGFSDVTSRAEDI
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            20563..22062
                     /locus_tag="ACHUOF_002040"
     CDS             20563..22062
                     /locus_tag="ACHUOF_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR0611223.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENGEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFGWDHPQAQVQLW
                     WDKDADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHDGNQHEKGGGEQLKGQY
                     ADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPIRPIARRT"
BASE COUNT         6001 a         5159 c         5304 g         5645 t
ORIGIN      
        1 gtgggcgtat ctttgaacca tttcgagagt ttcccatcct cccatctctt taagtgcaag
       61 aagagagaca ccggactgaa ccagccagct tgcccaggta tgcctcagat catggaagcg
      121 gaagttgcta atgcctgccc gctttaacgc tcccttccat gccttgttgc tgtcggttct
      181 catcttcctt accgctgctg tttttgttcc gtcgcttcgg taggcaggtt tggtgtggac
      241 aaatacccat ctcttatgga gcccctgctg ttttcttaat atctggcatg cggtttcgtt
      301 aagaggaact ccgatcgcat tgccagcttt tgtttcatca gggtgcatcc atgccatttt
      361 cttatccaga tcgacctgtg accactcaag gtctgtaacg ttggaacggc gaaggcctgt
      421 cgtgattgca aacatgacca cagggaagaa gtgaggagca atttctgcaa acaggcgctt
      481 cgattcctcc tctgtaagcc atctgatgcg tccattctta acgcgtggtg ttgatatttt
      541 gggtgccctg tcaagccatc cccattcaac agccatattg agaatggcgc gaagtattgc
      601 cagatgcctc gtcttcgttc ctttgcttgc aagctttggt ttatactccg gcaccggatt
      661 gccaagtcgc aaacacctgt cccggctcat ctcccagttc aggcgatggc ggcggttttc
      721 catcccgtct accgcctcca ttattttttc tgttgttatg tcagagagaa tggcttctct
      781 gaagtgcaac atccagaacg atataatgct cttgtcatca tcaatggact tcttatccga
      841 tttctcacgc agccaccgta tgcaggcttc cttgaatagc tttttcggtg attccccgag
      901 attttttact ctccacgctt ctgctttcag acgatcgtga agttcttgcg cttgcctttt
      961 gtccgatgtt tcaagagagc gtctaactct tgatccatct ggcgcgacga aatcgcagtg
     1021 ccacgtgcca ccgcgtagtt tgattgacat gctttaacct cctgcacatc aaccgcattc
     1081 accgcgctat tgtgtctcac agacttaagc gccgcaatgc agtctgactt gcaaatgcga
     1141 tatgggcttt taggtttatc tggatttatc tttgcggcct gaagtcgtcc acttcgtatc
     1201 cactgcgtga tagtgccttt gtctaccttc agatacgacg ctgcctcttc acgagtgaag
     1261 atttcttctt ccacctggaa tctccattta ttggattggt attattgcgg taggtctgga
     1321 tatcattgag caatgaacag gcctcatcga gtgtgaggcg ttagtccttg cgtagctcgc
     1381 tgattcttct gtaagtctct ggtgctttgt tcccgtacgt cttcatttca gacttcaaca
     1441 gagcaacgag tgaatcccat tcgttgagga ttcctttgaa tgccggaacg cgctttgcaa
     1501 ccttgtcgaa tgaatctctg atttctggaa tctgctcaac aagtgcaacg catcgccgga
     1561 aatcggctgc gtcatgtgga gcaccgaagc tatgaccata gatattcttt ttcagtccac
     1621 atgcgattga ggcaagagtt gcgctactga tgccgacatc gtcagtcgat tgccatttca
     1681 aaaccttcat agccaaatct gacatttctt gtctccaata aaaaaccgcc atcaggcggc
     1741 ttggtgttct ttcaattctt caattcgaat attggttaca ttgttttcat atatgaataa
     1801 ataaattagc ttttttcgtt gccttcgcgt tctttattaa ttttgacaaa ctcgttttta
     1861 ccacgctctc caaatgcgtc tttagagtcg ttgtatccgc aatcgcagca cacataatca
     1921 ccagaccatc cacgcattgt tttttctttt gcaatatttc cagaaccgca ttttggacaa
     1981 gacatatcac tacctccaaa gcatgagtga gatgacaacg taacattgat tggagattaa
     2041 caatagattg ctgatgtaaa agatatgtat aagcttcgct ttcaaagtgg aggctctggt
     2101 agcggcatcc agtgggttac attgctaatc agaccatatt cattagtttg aggatggttg
     2161 ccgttatcgt ctccgtattt aagactctcc ataaaaccat aatgcttatt cccattaacg
     2221 ctcacaaagc cgtaataagc tggtataacg ccgatctcac acgtaatcag caaaggaaaa
     2281 ctagttctcc aatttagctc gccgattaca ggcattctct cactacagct tatccaacca
     2341 tccggagtta ccggaacttg cggaatggct gtctgctctc gaacgtcatt aggcgcgata
     2401 ggttctgctg ccaactgact ggcatatttg ttaatggtaa cgataagctc ttgctcagcc
     2461 tcatccagac aatcaccgat acctcgcctg tcaccgtcaa aatcatcgaa atcggcacga
     2521 atcttggcaa ccttctggat tgcggacaac acctcactag gaattaccgg atagttggtt
     2581 gacgtttccg cgatttcacg aaaattattg gttgacgaat tcttgttttc ccgaaagttt
     2641 ccggactgaa gcatggctgc gcgataggcg ttccagccga cagcttttcc gtgttcaaac
     2701 gcgctgtcaa agtcatcatc catttccatc gcatcaggca cagataccgt ctctggaggg
     2761 gcggcgtaaa gcggagttac ggtaatttcg tactcgttta tatcctcgtc gctgatattt
     2821 gcgaaatagc gaaggatgcc tttctctcca cgactacgag cgtcagagtc ctgaatgata
     2881 tacgccacag gctctgcttc cagcgatgcc agcgcaattc gtgccagttc catttgttcg
     2941 ccacgagtaa gcccgttttc aagcggattt ttaatgaaca attcaatacg ttctttggta
     3001 atagtggtca tagctatttc accttaatct caatatttcg cagctttagc tctactggca
     3061 ggtctgactt tcctgttaac gctaatgcga gattttctgg agtaatgaga gcagttattg
     3121 ttttccccat cgccagacga ataatcagtc gtatctcgcg atcgtcacat gctcctggtc
     3181 gaacaattga tatttgtccg tccatctcac tctcctttga tgcgaatgcc agcggcgcgg
     3241 attgcagcga tgacctcaga aactttgtat gccattaccg tttggtaatc atcgtgaaaa
     3301 tctgttcgat gaagcatgct gctacgttcc gggagcagta ttccccgctc ccccagttct
     3361 gctatgcgat tctctgcgga ttccagcgcc gcaaccaatt cgtctacagt tccggcagct
     3421 tgcagtgcgt aatcggtaat agccatctca tgatcaattt cagtaccgtt ctcattcgtt
     3481 gaggtgatag caaaataatc agagtcgatt tcgttatcag ctaagtggcg tagcgtatcg
     3541 gcaacaagcc ggccgttttc gattagcagc ttccctaccg ttagcgcaat atcctcgttc
     3601 tcctggtcgc gggatttgat gtattgctga ttccttaccc gttcatccag tagtgccagc
     3661 acggtttctg gtccggccag aaatttgaag gcgttgagcg catcaatatc cacaccgtaa
     3721 tctttaagtt cctgttcact taacaaatca tcagctggca acattaacag gcgttccatt
     3781 gctggaattg cacgttccgc cgcctcacgc agtgcctgat agttaatttc gctcactggc
     3841 cgcctccttt gcgaagctgg gcggcgaact cgttagccat cgctgaatat gggtgctcat
     3901 gaccgccaac aaccttcatg ctggcagcaa acatctccac gccctgcgcc cgcacttcat
     3961 ccaggaaagt gtcggtggct ggggtttctg gaatggcgtc tggaataacc tctgagtaga
     4021 cgcgatccat tgccgactcc catccgtagt aacaagccga atagccgtcg cgctggtagc
     4081 cgtggtcttc aactccacat cccatgccct ggtcatggta ttcaggctga ttgtcgggat
     4141 taatgcagta ttcaatgacc gacttcatcc ccgcattctc cgccgccagc gccgaaaact
     4201 tctcgtgtgc caacttaaca gccgcatcag cctgcttaat tgactcaatc gctttctggt
     4261 ggtcttcgta cagagccgaa atcttggcct ccgcttcagc aaatttacgc accagatatt
     4321 cagcgtttgt ttcgttaacc tttaaatcac atgggatgca tttacctttc agaaaaccat
     4381 ccatctcaat tagtgacatt tgtttcattt cttcccactc cgccacatag cattcagata
     4441 tttgttgtca ttaacagaac cgaaactctt tctcttaagt aattcctctc tcgatggcat
     4501 tggctttacg cgttggcgaa taatcatttc tgccggaaga atgccgggat tgtatgcaag
     4561 tcctctcatg gtaaattcct cagtcattac tgatagcgcc atagcgtgag cggtaattac
     4621 gcaggcgcgg gtcaatttca gggaagtggg tatatgtggc tttgcggaat ggtcggattg
     4681 atgtctggta aattcgctcg agttcttctt tctctgcaag ccatatacag tggcgaaatt
     4741 ccttttcctc tttcgtttcc tgcggtagcg acattatccg atcgtagttt tttctgaatt
     4801 tatccagcac ctccgatacg gaattgccgg aacagcggcg tgggtcatcc gcaccataca
     4861 gaggcgctgg cataatggaa tccttatgtt gctactttag aagggaattg aatcgtcgta
     4921 ttcaggatga ttttgatgat tgctactttg ctgctgttgg ctgtttcctg aagttgcaaa
     4981 tccaatcttt gcattcagta attcaagagt gattgattgt ccattttgcc cctgataaac
     5041 atcaaccctg atgttttctc cggtaatttc cacaatgcca ccttcaacaa gaacactacg
     5101 gtagtaatcc gcttgcgctc ccggcttggc aaatacaacg gcgctgtagt ttgtccattc
     5161 tttctttttt gtctggcgat cgtaatactg aacgccagca cggatgttga atccgatatt
     5221 ttccccggcc tgaaactctc ttgcgggctt gtttagtctt acagtaatcg aatgtgccat
     5281 taagcagccg ctccttctaa ttcgtctcgt ctgatgttgt aaatgtcctg cgctttgtgc
     5341 tgctccggtg tgccttcgag catcttccac gctttggcga acgcctgttt aagctcttcc
     5401 acggtgtttt tctgcaatgc tgcgtcagtg aatgctttta gaacctgttc aggtgtaggt
     5461 gatggttttg attgctttgc tgctgcgttc tgctgatgtt tatgctcgtc ggtatctgca
     5521 tctttcgcat catcaatgcc gaacaaacca ttgaggcaat acttgcgtgc ataagagctt
     5581 gtggctcccg taacttgtgc agaatccatt cctttcttgc tttcttcctc tcgtgcaaga
     5641 gcggttgccg tatgactgtt ttcgccatcg gtaatagttg ccgtggcttt cacgtaatac
     5701 cgatcaccaa tcaacacaac ttcatcgctg attgataaaa acagaccatt cagtaacggc
     5761 ttaacgcctt caagaatgtc ttcgcagctt ctgtatttat atttaccgaa tgagttgtac
     5821 tggttctttg gcgcgttcaa gttctcctga atagctgcca gtcttgcgta aaattctttg
     5881 ctcatatgtt tgttctcaga atgggcatgg cccaaggaaa taacgctgat ttaatacttc
     5941 gactcgggac aaattaaggc atacccgcat tccttcgcgg tcgccattat ggcgatacca
     6001 gagagctttc tgcgtgtaca tgcgtctctg taacttgctc tccttcactg tggttgcaag
     6061 tgacatgaat atctccttcg ttaccgatta attctttcat ctgacgaatg aattcttcgt
     6121 ctgaccagtt atctgtaaaa ctcatttcct gcgataccac ggaatattga tagctgattt
     6181 catcgcttta tttgcttcaa gccacatttt tgaatcacca ataaatctgg ctattactgc
     6241 tttgttttgt gctgcacgaa gcatctggtg attgatggct atttcattgc gcataacgcc
     6301 tccagttgtt tctttgctgc actgattaat tgtttaactc ggcgtgataa ttcagattcg
     6361 tgcgggtaga aagcggacat gacgccgcta cccgcgagct gaaagtgcat catggataac
     6421 tccttctgtt tgattgcata acgaaaacgc ctcgagtgaa gcgttattgg tatgcatata
     6481 aaaaagccct catactggag ggcaaagaag atttcaaata atcagaacaa gtcggctcct
     6541 gtttagttac gagcgacatt gctccgtgta ttcactcgtt ggaatgaata cacagtgctt
     6601 actcgtacta ataaaatacc caattttctg tttcttggtt gtgcccaaag ttatattcaa
     6661 tatctggtgt tgatgtatca atattcttca tcccatcaac aagagttgat acaacagcca
     6721 aatcctgttt gattctcatt aaatggtatt tcttccggcg caataaactt tcaatggcaa
     6781 gtttcttcgt tgggaatgca aaagatcttt ctgcattttt tgctactttc ttaattgcat
     6841 atctatttct cttttgtttc cattcctgta accactgatt tggtgctggt ttaaaattaa
     6901 caatccaatg cgcaggaacc aaccatgcat aatgctctgt ctgatgaaaa gctatatatt
     6961 gaagtgcgaa tattttgatt ccatcttctt caactgtcgc ctggaatctc cagaaaacag
     7021 gcattccatc atgttcagtt tctgattcag gaaaaggtac gctccatgat tttgtcatat
     7081 ctcacctcaa ataagtggct tgctgcctaa tttcattttc tggcgaccaa cacaagtcac
     7141 acccatttca ctgcgtggct tgctgtacca tgtgcgctga ttcttgcgct caatacgttg
     7201 caggttgctt tcaatctgtt cgtggtattc agccagcacc gtaaggtcta tcggattcag
     7261 tgcgctttct actcgtgatt tcggtttgcg attcagcgag agaatagggc ggttaactgg
     7321 ttttgcgctt accccaacca acaggggatt tgctgctttc cattgagcct gtttctctgc
     7381 gcgacgttcg cggcggcgtg tttgtgcatc catcttgatt ctcctgtcag ttagctttgg
     7441 tggtgtggta ggtgggagac ccatttcgac ctgcttcggc cgacttcaat tcggcaatag
     7501 ttccgcaggc ctcgccgctt tacgtgcgac atattcccat ccatgaaccc ttcaccacac
     7561 cccaaagcct tctgctttga atgctgccct tcttcagggc ttaattttta agagcctcac
     7621 cttcatggtg gtcagtgcgt cctgctgatg gcttaaaatt acaagaaaga ttgtatgttg
     7681 taaacaagaa atattgtaaa aaggggcgta aaaacaaact ccattgtttt taaatgaaaa
     7741 atagtttgtt tttggttatc gagattgagg tggggattac tggttgcagg ttccgactac
     7801 atcaccaaca aatgatttgg ttgatgtaag ttgttgcata cctgggatat tcattacttt
     7861 ggagtaaaga gcttttttgt ctgtagtgat tgatcaagtt tcaacggtta ttcctcctcc
     7921 agactggtat tctcctacca tagtgttcga tgacaaagca gtgtatttca tctctggata
     7981 gacgccagaa actgattcat aaactgatga tttatcgcca tttattgtta cgtggaaaac
     8041 ggaatcttcc gtgctgtctt ttgtaaactc gtaacgatcg ccattcattg acccgtaccc
     8101 gtgcaggttt gtgacaatcc agcattcaga attggcgctg gtagttaaga gtattgagag
     8161 tagcgccgca atcctgatca tacgaatttt accctcgctt ccacgacaac accgataatc
     8221 ttgcagttcc cgttgatagg agtcataggc catgaagggt tcaggccttt caggtacttc
     8281 tgcccgccat ctataaccag tttcttgaat gttgcttcgt tcgcgtcagt cagtttggct
     8341 acaacaaggc ttccattcac tggctcgcgt ccagtatcta ctaacaccat atgaccttca
     8401 gggatgcttt gacctacagg tgaggtcatg gaatcacctt caaccttcag ccagaatcca
     8461 tcgcctaata agttaacgtc actgtcatac cattcatcaa tgtccttgat atcgtagggt
     8521 tcacaagctt cacaccacga accagctcta accatgctaa tcaatggata tttccctttg
     8581 ggctcaacat gcccaacaaa tctaacattc gaatcagagg tgccattgag cagccagtca
     8641 acacttacgc caagagctga cgcaagttct ggtaaaaagc gtggtcgctt agttttaccg
     8701 ttttcgagct gctctataga ctgctgggta gtccccacct tttgagcaag ttcagcctgg
     8761 ttaagtccaa gctgaattct tttgcttttt accctggaag aaatactcat aagccacctc
     8821 tgttatttac ctccaatctt cacaagaaaa actgtatttg acaaacaaga tacattgtat
     8881 gaaaatacaa gaaagtttgt tgatggaggc gatatgcaaa ctctttctga acgcctcaag
     8941 aagaggcgaa ttgcgttaaa aatgacgcaa accgaactgg caaccaaagc cggtgttaaa
     9001 cagcaatcaa ttcaactgat tgaagctgga gtaaccaagc gaccgcgctt cttgtttgag
     9061 attgctatgg cgcttaactg tgatccggtt tggttacagt acggaactaa acgcggtaaa
     9121 gccgcttaag acattcccgc tcttacacat cccagccctg aaaaagggca ttaccagaaa
     9181 caaatctcta tggttttgcg tttctttgcg aagccaactc tatctaatca ttaaggaaat
     9241 tatctatggg tactattgca actaaaagca agaaagcggc tcgcatcgag tcagccttgc
     9301 tgaacaaact ggcactgatg gggcagaaga cattcgctcg agcaatgggg gttcctgaat
     9361 atcaggtaag ccgatggaag aatggtttct tctcgcaggt aagcatgatg cttgctgttc
     9421 tggaatacgg aatcgaagac gatgaaatgg ctgaattgac taagcggctt gccgattacc
     9481 tgacaaaaga aaaagccccg aagaacggcg aattcttcga ggcctaatgt agaaagactg
     9541 gatcaatcca cgggagtaat tatgacaaaa cgtcgtaaga aataccagga aaaagaagag
     9601 attcgacacc ctgattcacc tgagggatta gtggtagccg cagcaaataa cagggcgttc
     9661 gcagagcgcc ttgttggtgt ttacagacta gccaaagcag gagtgaaaca tgggcgtcgt
     9721 taagttagct gattacaggc ctcaactgga ggtcgtggag catcgcgtgg cagaactcga
     9781 agatggctac actcggactg caaacacact gttagaagcc gccatgcttt ctggacttac
     9841 tctacatcag ttactgattg ttatggctgt gtggcgcaag acatacggtt acaacaaaaa
     9901 aatagattgg atcggaaacg aacagttcgc tgaactcact ggcatggcac caaccaaatg
     9961 ctctaccgcc aaaaacgagc ttatcagaat gggagttctc attcaggtgg ggcgtcaagt
    10021 tggtatgaat acaaacattt ccgagtggaa aacgaaagtt aacggattcg gtaaaacatt
    10081 taccaattcg gtaaaacaaa ccttcaccaa atcggtaaaa agcaatttac cgaatcagtc
    10141 aaacacaaaa gacaatatac aaaagacaat aaatacaaat acccccttac cccctaacgg
    10201 gggcggcgat gggcaggtta aacctgaacg tcgcaaggca gaacgaatcg actacgaatc
    10261 cttcctgaac gcctacaaca ccgaagtcgg tgacagactg ccacacgctg ttgcggtcaa
    10321 cgagaaacgc aaacgccgcc tgaagaaaat catcccgcaa ctgaaaacgc caaacgtgga
    10381 cggtttcaga gcgtatgtca gggcgtttgt gcatcaggcc aagccgtttt acttcggaga
    10441 caacgacacg ggctggacgg ccgattttga ttacctgctg agggaagact cgttaacggg
    10501 ggtacgggaa gggaagtttg cagacagggg gattgcatga aacaggatat cgaagcgagc
    10561 gttatcggtg gcctgctgat tggtggatta acaccaaccg ccagcgacgt tctggcaacg
    10621 ctggagccgg aagcgttttc aattccgctc taccggaaag ccttcgaggt tatccgcaag
    10681 caggcgcgaa acagaaacct aatcgacgcg ctgatggttg ccgaggcgtg cggagaggag
    10741 catttcacgt caatcctgat gaccagcaag aactgcccga gtgccgcaaa cctgaaggga
    10801 tatgctggaa tggtcgcgga taactatcac cgccgtctgg tgctggaaat catggatgaa
    10861 atgcgtgaac caatccaaag cggaaccatc gacgcatcga gtcaggcgat ggatgaactt
    10921 gtaaagcgtc tttcagccat cagaaagccc cgtgacgagg taaaacctgt acggttaggg
    10981 gaaatcatca ctgactacac tgacacgctt gacaggcgtc tgaggaacgg agaagagtca
    11041 gataccctga agaccggaat cgacgaactt gacgccatca ccggagggat gaacgcagaa
    11101 gacctggtga ttatcgccgc tcgtcctggt atgggtaaaa ccgaactggc gctgaagatt
    11161 gccgaaggcg ttgcaagccg cgttattcct ggttctgacg tccggcgcgg agtgttgatt
    11221 ttctcgatgg aaatgagcgc attgcagatt gcagagcgga gcattgccaa cgccgggagg
    11281 atgtcggtta gcgtgctgcg aaatcctgca tcgatggatg acgaaggctg ggcgcgtgtt
    11341 gctaacggca tgagtcagct tgcagatttg gatgtatggg tagtcgatgc ctcgcggtta
    11401 tcggtcgaag aaatacgctc aatcgcagaa cggcacaaac aggaaaatcc aaacctgtca
    11461 ctcatcatgg cggattatct tggcctgatt gagaagccga aagcagaccg caacgacctc
    11521 gcaattgctc acatctccgg aagcctgaag gcgatggcga aagacctgaa aacgccagtt
    11581 atctccctga gtcagctttc gcgcgatgtt gagaagcgac caaacaaacg cccgacaaac
    11641 gcagatttgc gtgattcagg aagcattgaa caggacgcag actcaatcat catgctctat
    11701 cgggaagcgg tatatgacga gaacagtagc gccgcgccat ttgctgaaat catcgtgacg
    11761 aaaaaccgtt ttggctcgct tggtacggtt taccagcggt tctgtaacgg acactttgtt
    11821 gcatgtgacc aggatgaagc cagacagatt tgcacagcat caaatgcacc tgctgcgcgt
    11881 ggcagacgat atgcacaagg ggctgacgta tgaccatcta catcactgag ataataacag
    11941 gcctgctggt aatcgcaggc ctttttattt gggggagagg gaagtgtggc tgactggcaa
    12001 attccaatca tcattcttgc cggagcttcg ctggttgctg gctttatcct gctgaaaaag
    12061 cataaagacc gtgatcaaaa agttgaagtt ctctatgggt atccagcgaa cagcacaaca
    12121 tggctgacca tttaccacta ccgaaaatca ggacgctggg tattcgaatg ggatgatctg
    12181 ttcgctgaaa agcgaccaaa gtcatgggga gacatcagcg aatgcatgat gtttgaagaa
    12241 agaaaatccg gcgcaacccg agaagagttt aacgaagcgt gggcgcgatt aagtgagaga
    12301 gggtatttgt gagcaagtac gaaaaattag atcaaaacat tctttcaatg ctgagtgaaa
    12361 gaccaacacc tgtttttgat atctggctta aatggcggag caatggaatg tatatcgaaa
    12421 ccatcgatcg ccgtatgcaa tacctgagaa agaaagggct tgttgcaaat gtgcgtggga
    12481 agggttgggt gaaaattaac ctgtcataac ggggattgat atggacgata gcttagaaat
    12541 atttgaagag tggtgcgctg atgaacttgg tgtctccgct tacttcattc gacaaatgcg
    12601 aagcaagaat attttgggcg tgattgagta taaacgagtt gaaataaaca agagatacag
    12661 ggcttggatg gccgcagttc gcgccgccgg agtcaaagtg aaggagtgag tatgagcgca
    12721 tacgaagaaa tcatgttagc cctgcgattc tttttcggtg tggaagaaga tgaaaacgta
    12781 aatgagatta tcggacaaga ccatgacccg atagggacta ttgcagctgc acttgacgat
    12841 tacaggagcg taaatggtga ggaaactaac gtttgaacta agaagcccca tccatcagca
    12901 gaacgccatt caagctatcc agcaaatcct tccagaccca accaaaccaa tcgtagtaac
    12961 cattcaggaa cgcaaccgca gcttagacca gaatcgaaag ctttgggctt gccttggtga
    13021 cgtttcgcgt caggttgaat ggcatggtcg ctggctggat gcagaaagct ggaagtgtgt
    13081 gtttaccgca gcattaaagc agcaggacgt tgttcctaac cttgccggga atggatttgt
    13141 ggtaataggc cggtcaacca gcaggatgcg tgtaggcgaa tttgcggagc tattagagct
    13201 tatacaggca ttcggtacag aacgtggcgt taaatggtca gacgaagcgc gactggctct
    13261 ggagtggaaa gcgagatggg gagataaaac tgaatgaaca aataccgact tatttacgca
    13321 gatccgcctt ggcaatatcg cgacaaagcc aacgatggca atcgcggtgc tggacataaa
    13381 tacgatgtta tgaatgttca ggacatttgc cgactgccag tatgggattt agcggatcca
    13441 gaatcttgct tgttagcgat gtggtgggtg ccgacacagc cagccgaagc gctaaaggta
    13501 attgaggcgt ggggattcag gttgatgact atgaaaggct ttacttggca caaaaccaat
    13561 aagcacaaag gcaacagtgc gatcggaatg gggcatatga ccagggcaaa tagcgaggat
    13621 tgcttgtttg ccgttcgagg gcggttacct gagagaatgg acgcttcaat atgccagcac
    13681 tttaccgcac cgagaatgga gcacagtgca aaaccaccga tcgtaagaga catgttagct
    13741 aagttgcttg gcgacgtgcc gcgctgtgag ttatttagcc gcgacaaagt gaccgggtgg
    13801 gatatgtggg gaaatcagtg cgactccgat tttgaacttg ctcccggcat ggcgattaaa
    13861 ccatgcaaaa tggtgatcgc atgaagcact gttatcgatg tggagagcga aaggaagacg
    13921 atcgctttcg accaggacag ccttactgga atcgatggtg tctccggtgt gaaagaacac
    13981 caacaggggt gttaccacta ccgcaggaaa aggaggacgt gtggcgagac agcgacgaag
    14041 tatcaccgac ataatctgtg aaactgcaaa taccttccaa cgaaacgctc cagaaataaa
    14101 cgcaagccaa tcccaaaaga atctgacgta aaaaccttca actacacggc tcacctgtgg
    14161 gatatccggt ggctaagaca tcgtgcgagg aaatgacaat gcttttaatt caacctggat
    14221 ttggacttag catcaaaaaa ggccacatgt ttggcgagaa agagtcacaa cgaaaaatga
    14281 tgtctatccg gttgccattt atcagtattt attggctaaa cagggaggca acaaattatt
    14341 ggtatacatg cgccagagca gcatttaacg accctgactg gtttgtggaa aaccaccacg
    14401 cagttcgtca ggcaaagaga aaggccaaca tgacatacat gaaggcgtat cgaaaagcat
    14461 ggaaagaaca ccgcgatcga taccagcaag atatggaaaa gcttgaatca gaaaacatgg
    14521 aattaagacg aaagcttggt gaagcaaaac gagacattga tgcttacaag cgacttttta
    14581 atggtgaaag ccatgcttag tccatcccaa tctcttcaat accagaaaga aagcgtcgag
    14641 cgggctttaa cgtgcgctaa ctgcggtcag aagctgcatg tgctggaagt tcacgtgtgt
    14701 gagcactgct gcgcagaact gatgagcgat ccgaatagtt caatgtacga ggaagaagac
    14761 gatggccaaa ccagcgcgaa gacgatgtaa aaacgaagaa tgtcgggaat ggtttcaccc
    14821 tgcattcgct aatcagtggt ggtgctctcc agagtgtgga accaagatag cactcgaacg
    14881 acgaagtaaa gaacgcgaaa aagcggaaaa agcagcagag aagaaacgac gacgagagga
    14941 gcagaaacag aaagataaac ttaagattcg aaaactcgcc ttaaagcccc gcagttactg
    15001 gattaaacaa gcccaacaag ccgtaaacgc cttcatcaga gaaagagacc gcgacttacc
    15061 atgtatctcg tgcggaacgc tcacgtctgc tcagtgggat gccggacatt accggacaac
    15121 tgctgcggca cctcaactcc gatttgatga acgcaatatt cacaagcaat gcgtggtgtg
    15181 caaccagcac aaaagcggaa atctcgttcc gtatcgcgtc gaactgatta accgcatcgg
    15241 gcaggaagca gtagacgaaa tcgaatcaaa ccataaccgc catcgctgga ctgtcgtaga
    15301 gtgcaaggcg atcaaggcag agtaccaaca gaaactcaaa gacctgcgaa atagcagaag
    15361 tgaggccgca tgacgttctc agtaaaaacc attccagaca tgctcgttga agcatacgga
    15421 aaccagacag aagtagcacg cagactgaaa tgtagtcgcg gtacggtcag aaaatacgtt
    15481 gatgataaag acgggaaaat gcacgccatc gtcaacgacg ttctcatggt tcatcgcgga
    15541 tggagtgaaa gagatgcgct attacgaaaa aattgatggc agcaaatacc gaaatatttg
    15601 ggtagttggc gatctgcacg gatgctacac gaacctgatg aaaaaactgg agacgatagg
    15661 attcgacacc aaaaaagacc tgcttatctc ggttggcgat ttggtcgatc gcggtacaga
    15721 gaacgtcgaa tgcctggaat taatcacatt cccctggttc agagctgtac gtggaaacca
    15781 tgagcaaatg atgattgatg gcttatcaga gcgtggaaac gtcaatcact ggctacttaa
    15841 tggcggcggc tggttcttta atatcgatta cgacaaagaa attctggcta aagctctttc
    15901 ccataaagca gatgaacttc cgttaatcat cgaactggtg agcaaagata aaaaatatgt
    15961 catctgccac gccgattatc cttgtgacga atacgagttt ggaaagccag ttgatcatca
    16021 gcaggtaatc tggaaccgcg aacgaatcag caactcacaa gacgggatcg tgaaagaaat
    16081 caaaggcgcg gacacgttca tctttggtca tacgccagca gtgaaaccac tcaaatttgc
    16141 caaccagatg tatatcgata ccggcgcagt gttctgcgga aacctcacat tgattcaggt
    16201 acagggagaa ggcgcatgag actcgaaagc gtagctaaat ttcattcgcc aaaaagcccg
    16261 atgatgagtg actcaccacg ggccacggct tctgactctc tttccggtac tgatgtgatg
    16321 gctgctatgg ggatggcgca atcacaagcc ggattcggaa tggctgcatt ctgcggtaag
    16381 catgaactca gccagaacga caaacaaaag gctatcaact atctgatgca atttgcacac
    16441 aaggtatcgg ggaaataccg tggtgtggca aagctcgaag gaaatactaa ggcaaaggta
    16501 ctgcaagtgc tcgcaacatt cgcttatgcg gattattgcc gtagtgccgc gacgcctggc
    16561 gcaagatgca gagattgcca cggtacaggt cgtgcggttg atatagccaa aacagagcag
    16621 tgggggagag ttgtcgagaa agagtgcgga agatgcaaag gcgtcggcta ttcaaggatg
    16681 ccagcaagcg cagcatatcg cgctgtaacg atgctaatcc caaaccttac ccaacccacc
    16741 tggtcacgca ctgttaagcc gctgtatgac gctctggtgg tgcaatgcca caaggaagag
    16801 tcaatcgcag acaacatttt gaatgcggtc acacgttagc agcatgattg ccacggatgg
    16861 caacatatta acggcatgat attgacttat tgaataaaat tgggtaaatt tgactcaacg
    16921 atggataaat gcactcgtta aataaagccc tgagttaata gctcggggct ttttgcgttt
    16981 taagcacggc ctttctgaaa gcacatcaag ccaaatacca gacagacaaa aataatcacc
    17041 ttatccgctt ggctacggtg cggtgtgctt tgcataaaag aaaaccagcg caatggctgg
    17101 cttcgtgaaa gcgggtggca agaggctgcg ctaacaacct catgccgttt tgcccgtgca
    17161 tatcggtcac gaacaaatct gattactaaa cacagtagcc tggatttgtt ctatcagtaa
    17221 tcgaccttat tcctaattaa atagagcaaa tccccttatt gggggtaaga catgaagatg
    17281 ccagaaaaac atgacctgtt agccgccatt ctcgcggcaa aggaacaagg catcggggca
    17341 atccttgcgt ttgcaatggc gtaccttcgc ggcagatata atggcggtgc gtttacaaaa
    17401 acagtaatcg acgcaacgat gtgcgccatt atcgcctggt tcattcgtga ccttctcgac
    17461 ttcgccggac taagtagcaa tctcgcttat ataacgagcg tgttcatcgg ctacatcggt
    17521 actgactcga ttggttcgct tatcaaacgc ttcgctgcta aaaaagccgg agtagaagat
    17581 ggtggaaatc aataatcaac gtaaggcgtt cctcgatatg ctggcgtggt cagagggaac
    17641 agataacgga cgacagaaaa ccagaaatca tggttatgac gtcattgtag gcggagagct
    17701 atttactgat tactccgatc accctcgcaa acttgtcacg ctaaacccaa aactcaaatc
    17761 aacagcagcc gggcgctatc agcttctttc ccgttggtgg gatgcctacc gtaagcagct
    17821 tggcctgaaa gacttctctc cgaaaagcca ggacgctgtg gcattgcaac agattaaaga
    17881 gcgtggcgct ttgccgatga ttgatcgcgg tgatatccgt caggcaatcg accgttgcag
    17941 caatatctgg gcttcgttgc ccggggctgg ttatggccag ttcgagcata aggctgacag
    18001 cctgattgca aaattcaaag aagcaggcgg aacggtcaga gagattgagg tatgagcaga
    18061 gtaaccgcga ttatctccgc tctggttatc tgcatcatcg tctgcctgtc atgggctgtt
    18121 aatcattacc gtgataacgc catcgcctac aaagagcagc gcgacaaagc cgcatccact
    18181 atcgctgata tgcagaagcg tcaacgtgac gtagcagaac tcgacgccag atatacaaag
    18241 gagcttgctg atgctaacgc gactatcgaa agcctccgtg ctgatgtttc tgctggtcgt
    18301 aagcgcctgc aagtcgccgc cacctgtgca aagtcaacga ccggagccag cagcatgggc
    18361 gatggagaaa gcccaagact tacagcagat gctgaactca attattaccg tctccgaagc
    18421 ggaatcgaca ggataaccgc gcaggttaac tacctgcagg aatacatcag gactcagtgc
    18481 ctgaaataat ttttttgcaa atcacaaagt ccatttaatg agcctcgcga tgcggggctt
    18541 ttttacatct gaatttcaca gcgcatctca cgcgcatatt acatcacccg agcctttcag
    18601 aaagttgagc ctgagaactg ccgtatatgg tggcgaccat ctcgggacgg cttttctgtg
    18661 agacaggctc actttctaaa aggtaaagac gcaatgaact acccaaccgt tgttaacgat
    18721 atagatttca gagacctaat ttttgtagca aacaacgatc cggttacaga ttcttttatg
    18781 gtggcaaaag catttggaaa gctgccgaag aacgtagttc gtgacattga gcgaaccata
    18841 gaagcttgcc ctcctgagtt tgatacaaag ctcaactttg agctttgcta taaaaacaat
    18901 gagttacaga atggtaagcc gcaaaaattc taccgtctcc gcaaggatgg gttgatgctt
    18961 ttggttatgt ccgacaccaa aaaagaagca atgcgtatca aaattgctta catcaacgca
    19021 tttaactgga tgtacgccat gcttcaggtt ggtcatcgtc aatttgaaga agagagaaat
    19081 gccgtaatgc tggagtacat gaaagagaag gatgttgcca gcatgtcagg tcgcctgcta
    19141 aatcgctggg gcaaaattaa gaagccacag ctgatggcta gaattgaacg ccttgaacag
    19201 cacgggcaaa ccgtaatccc cggactcatc aattaacggc agcaccgcga aacaacccaa
    19261 gccagtaagt ggggaaataa cactggcagc cactgaaaga tgaacctcca gccttatggc
    19321 aaaaaagatt ctttgtggtg gcggactgat ggaaagacat cggatagaat caagcagtgg
    19381 ctagggtagc tcccgaaaag cggactcgtc accgcctgcc actgaatcta tggcgaacaa
    19441 ctagacgagg ttgtgatgga tgataacgga aaccaatggg tcaatgttga acaccggcta
    19501 ccagaaagca aagaagggat gtggtctaaa gaggttatag ctcttactga tactggtgac
    19561 gtgttcaaac tatcatgtat gggctcttac tggcagagaa ccaaggcatt catcgattca
    19621 ggagcaagca aggttacaca ctggatgccg cttaattacc cagatgatta aaacggatga
    19681 aaaatgaaag gtcgctcagg cggccttttt tattgccatc acaaaagcca ttccttactg
    19741 agtggctttg ataatggctt ataccctaca cgggataact taactgatat cccttttaac
    19801 ggataaatgg agccaacaat ggcagagatt attcccatga ctgaagaaca gaaattccag
    19861 ttagagattt acaaactggt catgaaccag aacgcagccg cagaagaagc atttcaattc
    19921 attggcactg acgaactgaa gcttgagcta ttcaaaattc acttccagtc aggtggcgct
    19981 aattcagata tcacgatccg cacatttgaa gcggtgcgta aatcgaagga agcgttagac
    20041 ctgttcacca ccggagcatg atgctcaacc tgaaataacg attaagtgag atgaatatgg
    20101 cagcaccaaa gggcaaccga ttctgggagg cccgcagtag tcatgggcga aaccctaaat
    20161 tcgaatcgcc tgaggcgctg tgggctgctt gttgtgaata cttcgagtgg gctgatgata
    20221 acccgctatg ggagggtaag gtattttcat atcagggaga aataattaag gctaatgtcc
    20281 ctaagatgcg agccatgact atttcaggat tgtgtacctt ccttgatatc accaggcaaa
    20341 catggggaac cttccggtca atggaaggtt tttctgacgt cacatcacga gcggaagaca
    20401 tcatctacga ccagaaattc tctggcgcag ccgctgacct tctcaacgct aacatcatcg
    20461 cccgtgattt gggcctcaaa gagcagtcgc aagttgaaga cgtgacacct gataagggag
    20521 atcgcgataa gcggcgctct cgtatcaagg agctattcaa ccgtggaact ggacgcgatt
    20581 cttgataact tgagcgacga agagcaaatc gaattgctcg agctactcga agaagaagag
    20641 aactaccgta acacacacct gctatatgaa tttacgccat acagcaaaca gcgtgagttc
    20701 atcgacgccg ggcatgacta tccagagcgc tgttttatgg ctggtaacca gcttggtaag
    20761 tcatttactg gtgctgctga agtcgcgttt caccttaccg ggcgttatcc gggcacaaaa
    20821 ggctatcctg ctgatggtaa atatggcggt gagtggaaag gtaagcgttt ttatgagcct
    20881 gttgtctttt ggattggtgg cgagacaaac gagactgtaa ccaaaacgac tcaacgcatc
    20941 ctgtgtggtc gtatcgaaga gaatggtgag ccaggctacg gttccatacc taaagaagac
    21001 atcattagct ggaagaagtc tcctttcttt ccgaaccttg ttgatcacct tctggttaag
    21061 catcacacgg ctgatggcgt tgaagatggc atttcaatct gctacttcaa gccatactcg
    21121 caaggtcgtg ctcgctggca gggtgacaca atccacggcg tgtggtttga cgaagagcca
    21181 ccatacagca tttatggtga aggccttacc cgtaccaaca aatacgggca attctcaatt
    21241 ctgacgttta ccccgctgat ggggatgtct gacgttgtta ccaagttcct gaagaatcca
    21301 agcaagtcgc agaaagtggt caacatgacc atctacgacg ctgagcacta cacagacgaa
    21361 cagaaagagc aaatcatcgc atcttatcct gagcatgaga gagaggcgcg tgctcgcggt
    21421 attcctacga tgggtagtgg tcgaatcttc cagataccgg aagagacgat taagtgtcag
    21481 ccgttcgagt gtcctgatca cttctacgta attggcggga tggatttcgg atgggatcac
    21541 ccgcaggcgc aggttcagct ttggtgggac aaggacgcag acacaatata cgtttcacgc
    21601 gtgtggaagg cgaaagaaaa aacagccgtt caggcgtggg gagccgttaa atcatgggcg
    21661 cataaagtgc caactgcatg gcctcatgac ggaaaccagc acgagaaggg cggcggtgag
    21721 cagctcaaag ggcagtatgc agatgctggt tttatgatgt tgcaggagca tgcgacatgg
    21781 cctgatggcg gtaatgctgt ggagcctggc atcactgaat tgcgcgacat gatgctcgat
    21841 ggtcgcttca aagtattcaa cacctgtgag ccattctttg aggagttccg cctctatcac
    21901 cgtgatgaaa acgggaaaat cgtcaagctt aacgacgacg ttctctcagc cgttcgctat
    21961 gcatacatga tgcgccgctt cgccaaaatg atgcgcgaca tcaaaaaacc aaaagagaaa
    22021 aagataccag ccccaatcag gcccatcgca cggagaactt aaatggccga cgaaaacaga
    22081 ctcaattcca ttctgtgtaa gtttgacgc
//
LOCUS       DAZOYF010000057        21721 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZOYF010000057 DAZOYF010000000
VERSION     DAZOYF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.057"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..79
                     /locus_tag="ACHUOF_002041"
     CDS             <1..79
                     /locus_tag="ACHUOF_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611224.1"
                     /translation="RAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHUOF_002042"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHUOF_002042"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR0611225.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="ACHUOF_002043"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="ACHUOF_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR0611226.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHUOF_002044"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHUOF_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR0611227.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHUOF_002045"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHUOF_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR0611228.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHUOF_002046"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHUOF_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR0611229.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="ACHUOF_002047"
     CDS             5377..5835
                     /locus_tag="ACHUOF_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR0611230.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="ACHUOF_002048"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="ACHUOF_002048"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR0611231.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="ACHUOF_002049"
     tRNA            6887..6971
                     /locus_tag="ACHUOF_002049"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="ACHUOF_002050"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="ACHUOF_002050"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHUOF_002051"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHUOF_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR0611232.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHUOF_002052"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHUOF_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR0611233.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHUOF_002053"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHUOF_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0611234.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="ACHUOF_002054"
     CDS             complement(10252..13995)
                     /locus_tag="ACHUOF_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR0611235.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="ACHUOF_002055"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="ACHUOF_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR0611236.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="ACHUOF_002056"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="ACHUOF_002056"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR0611237.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="ACHUOF_002057"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="ACHUOF_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR0611238.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="ACHUOF_002058"
                     /gene_synonym="iatA"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="ACHUOF_002058"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR0611239.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="ACHUOF_002059"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="ACHUOF_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR0611240.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="ACHUOF_002060"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="ACHUOF_002060"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR0611241.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="ACHUOF_002061"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="ACHUOF_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR0611242.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
BASE COUNT         5463 a         5719 c         5240 g         5299 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 a
//
LOCUS       DAZOYF010000058        21512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZOYF010000058 DAZOYF010000000
VERSION     DAZOYF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.058"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACHUOF_002062"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACHUOF_002062"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR0611243.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="ACHUOF_002063"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="ACHUOF_002063"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR0611244.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACHUOF_002064"
     CDS             complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACHUOF_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR0611245.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="ACHUOF_002065"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="ACHUOF_002065"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR0611246.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="ACHUOF_002066"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="ACHUOF_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACHUOF_002067"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACHUOF_002067"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR0611247.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACHUOF_002068"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACHUOF_002068"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR0611248.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACHUOF_002069"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACHUOF_002069"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR0611249.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACHUOF_002070"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACHUOF_002070"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR0611250.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACHUOF_002071"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACHUOF_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR0611251.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="ACHUOF_002072"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="ACHUOF_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR0611252.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="ACHUOF_002073"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="ACHUOF_002073"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR0611253.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFRSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACHUOF_002074"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACHUOF_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR0611254.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACHUOF_002075"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACHUOF_002075"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR0611255.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACHUOF_002076"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACHUOF_002076"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR0611256.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACHUOF_002077"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACHUOF_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR0611257.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="ACHUOF_002078"
     CDS             complement(18170..18565)
                     /locus_tag="ACHUOF_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0611258.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="ACHUOF_002079"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="ACHUOF_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0611259.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="ACHUOF_002080"
     CDS             19481..20074
                     /locus_tag="ACHUOF_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0611260.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="ACHUOF_002081"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="ACHUOF_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR0611261.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21512
                     /locus_tag="ACHUOF_002082"
     CDS             20794..>21512
                     /locus_tag="ACHUOF_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0611262.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5195 a         5324 c         5669 g         5324 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccggt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag
    21421 ttaaataatt cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct
    21481 acgattacgg atttattaga aaaagcattg aa
//
LOCUS       DAZOYF010000059        21275 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZOYF010000059 DAZOYF010000000
VERSION     DAZOYF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.059"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..444)
                     /locus_tag="ACHUOF_002083"
     CDS             complement(<1..444)
                     /locus_tag="ACHUOF_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR0611263.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(499..1350)
                     /gene="manZ"
                     /locus_tag="ACHUOF_002084"
     CDS             complement(499..1350)
                     /gene="manZ"
                     /locus_tag="ACHUOF_002084"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR0611264.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1363..2163)
                     /gene="manY"
                     /locus_tag="ACHUOF_002085"
     CDS             complement(1363..2163)
                     /gene="manY"
                     /locus_tag="ACHUOF_002085"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR0611265.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2226..3197)
                     /gene="manX"
                     /locus_tag="ACHUOF_002086"
     CDS             complement(2226..3197)
                     /gene="manX"
                     /locus_tag="ACHUOF_002086"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR0611266.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3514..3672
                     /gene="yoaL"
                     /locus_tag="ACHUOF_002087"
     CDS             3514..3672
                     /gene="yoaL"
                     /locus_tag="ACHUOF_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR0611267.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            3660..5210
                     /gene="yoaE"
                     /locus_tag="ACHUOF_002088"
     CDS             3660..5210
                     /gene="yoaE"
                     /locus_tag="ACHUOF_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR0611268.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5214..6812)
                     /locus_tag="ACHUOF_002089"
     CDS             complement(5214..6812)
                     /locus_tag="ACHUOF_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR0611269.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="ACHUOF_002090"
     CDS             complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="ACHUOF_002090"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0611270.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8491..9069)
                     /locus_tag="ACHUOF_002091"
     CDS             complement(8491..9069)
                     /locus_tag="ACHUOF_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR0611271.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="ACHUOF_002092"
     CDS             complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="ACHUOF_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR0611272.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10508..10687
                     /locus_tag="ACHUOF_002093"
     CDS             10508..10687
                     /locus_tag="ACHUOF_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR0611273.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10807..11106)
                     /locus_tag="ACHUOF_002094"
     CDS             complement(10807..11106)
                     /locus_tag="ACHUOF_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR0611274.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(11528..11872)
                     /locus_tag="ACHUOF_002095"
     CDS             complement(11528..11872)
                     /locus_tag="ACHUOF_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0611275.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12004..13914
                     /locus_tag="ACHUOF_002096"
     CDS             12004..13914
                     /locus_tag="ACHUOF_002096"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR0611276.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13972..14667
                     /gene="tsaB"
                     /locus_tag="ACHUOF_002097"
     CDS             13972..14667
                     /gene="tsaB"
                     /locus_tag="ACHUOF_002097"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR0611277.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14707..15288
                     /gene="yeaY"
                     /locus_tag="ACHUOF_002098"
     CDS             14707..15288
                     /gene="yeaY"
                     /locus_tag="ACHUOF_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR0611278.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15494..17179
                     /gene="fadD"
                     /locus_tag="ACHUOF_002099"
     CDS             15494..17179
                     /gene="fadD"
                     /locus_tag="ACHUOF_002099"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR0611279.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17261..18376
                     /gene="rnd"
                     /locus_tag="ACHUOF_002100"
     CDS             17261..18376
                     /gene="rnd"
                     /locus_tag="ACHUOF_002100"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR0611280.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="ACHUOF_002101"
     CDS             complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="ACHUOF_002101"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR0611281.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="ACHUOF_002102"
                     /pseudo
     CDS             complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="ACHUOF_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
     gene            complement(20410..21018)
                     /locus_tag="ACHUOF_002103"
     CDS             complement(20410..21018)
                     /locus_tag="ACHUOF_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0611282.1"
                     /translation="MTGEKESKNAERIFMPPEIMRFKERLEEAMNGESSRAFAAKCGL
                     SDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDR
                     STLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQ
                     TAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21188..>21275
                     /locus_tag="ACHUOF_002104"
     CDS             21188..>21275
                     /locus_tag="ACHUOF_002104"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0611283.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5265 a         5273 c         5465 g         5272 t
ORIGIN      
        1 gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg
       61 ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct
      121 atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat
      181 gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat
      241 tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat
      301 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
      361 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      421 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      481 cggtagtgta cgacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      541 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      601 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      661 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      721 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      781 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      841 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      901 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      961 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
     1021 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
     1081 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
     1141 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
     1201 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
     1261 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1321 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1381 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1441 aagttggatg tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa
     1501 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1561 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1621 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1681 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1741 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1801 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1861 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1921 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1981 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     2041 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     2101 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     2161 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     2221 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     2281 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2341 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2401 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2461 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2521 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2581 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2641 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2701 aggccaataa ccatgtagtc gtttggcccc attggttttg ccggagttgg agccgctttt
     2761 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2821 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2881 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2941 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     3001 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     3061 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     3121 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     3181 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     3241 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3301 cctgcgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3361 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3421 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3481 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3541 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3601 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3661 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3721 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3781 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3841 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3901 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3961 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     4021 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     4081 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     4141 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     4201 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     4261 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4321 cgatcattat cgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4381 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4441 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4501 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4561 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4621 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4681 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4741 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4801 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4861 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4921 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4981 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     5041 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     5101 atgtgattga tgtagggcca ctgcatatca ccatctttga agccaatgat tatcgtgttg
     5161 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     5221 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     5281 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5341 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5401 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5461 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5521 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5581 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5641 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5701 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5761 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5821 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5881 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5941 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     6001 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     6061 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     6121 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     6181 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     6241 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6301 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6361 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6421 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6481 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6541 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6601 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6661 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6721 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6781 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6841 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6901 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6961 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     7021 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     7081 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     7141 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     7201 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     7261 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7321 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7381 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7441 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7501 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7561 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7621 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     7681 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7741 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7801 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7861 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7921 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7981 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     8041 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     8101 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     8161 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     8221 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     8281 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8341 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8401 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8461 agtgttttgt agatgtaaag ataagtatag tcagggggtc acaccaattt gcagcgccag
     8521 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8581 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8641 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8701 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8761 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8821 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8881 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8941 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     9001 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     9061 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     9121 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     9181 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     9241 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9301 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9361 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9421 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9481 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9541 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9601 tggtagcgtg cctttaatcg ggcgagtctg gatttcacta ttatcacaaa gaataaaccg
     9661 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9721 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9781 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9841 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9901 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9961 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
    10021 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
    10081 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
    10141 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
    10201 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
    10261 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10321 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10381 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10441 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10501 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10561 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10621 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10681 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10741 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10801 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10861 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10921 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10981 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    11041 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    11101 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    11161 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    11221 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    11281 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11341 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11401 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11461 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11521 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11581 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11641 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11701 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11761 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11821 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11881 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11941 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    12001 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    12061 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    12121 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    12181 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    12241 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12301 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12361 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12421 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12481 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12541 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12601 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12661 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12721 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12781 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12841 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12901 gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12961 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    13021 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    13081 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    13141 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    13201 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    13261 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13321 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13381 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13441 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13501 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13561 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13621 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13681 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13741 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13801 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13861 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13921 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13981 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    14041 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    14101 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    14161 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    14221 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    14281 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgatg cgcgaatggg cgaagtttac
    14341 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14401 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14461 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14521 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14581 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14641 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14701 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14761 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtac cacgccgcaa
    14821 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14881 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14941 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    15001 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    15061 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    15121 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    15181 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    15241 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15301 aaagaatgca gaaacagcgg ctggtccgct gtttctgcat tcttacggta aagataaaaa
    15361 taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg tatgatgagt
    15421 taatattatg ttaacggcgt gtatatcatt tggggttgcg atgacgacga acacgcattt
    15481 tagaggtgaa gaattgaaga aggtttggct taaccgttat cccgcggacg ttccgacgga
    15541 gatcaaccct gaccgttatc aatctctggt agatatgttt gagcagtcgg tcgcgcgcta
    15601 cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg accttccgca agctggaaga
    15661 acgcagtcgc gcgtttgccg cttatttgca acaagggttg gggctgaaga aaggcgatcg
    15721 cgttgcgttg atgatgccta acttattgca atatccggtg gcgctatttg gcattttgcg
    15781 tgccgggata atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc ttgagcatca
    15841 gcttaacgat agcggcgcat cggcgattgt tatcgtgtct aactttgccc acactctgga
    15901 aaaagtggtt gataaaaccg ccgttcagca cgtaattctg acccgtatgg gcgatcagct
    15961 atctacggca aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc gcttggtgcc
    16021 gaaataccat ctgccagatg ccatttcatt tcgtagcgca ctgcataacg gctaccggat
    16081 gcagtacgtc aaacccgaac tggtgccgga agatttagcc ttcctgcaat acaccggcgg
    16141 caccacaggt gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg cgaacctgga
    16201 acaggtcaac gccacctatg ggccgttgct gcatccaggt aaagaactgg tagtaacggc
    16261 actgccgctg tatcacattt ttgccctgac cattaactgc ctgctgttta tcgaactggg
    16321 tgggcagaac ctgcttatca ctaacccgcg tgatattcca gggttggtaa aagagttagc
    16381 gaaatatccg tttaccgcta tcactggcgt taacaccttg ttcaatgcgt tgctgaacaa
    16441 taaagagttc cagcagctgg atttctccag tctgcatctt tccgcaggcg gtgggatgcc
    16501 tgttcagcaa gtggtggcag aacgctgggt gaaactgacc ggacagtatc tgctggaagg
    16561 ttatggcctg accgagtgcg cgccgctggt gagcgttaac ccgtatgata ttgattatca
    16621 tagcggtagc atcggtttgc cggtgccatc gacggaagcc aaactggtgg atgacgacga
    16681 caacgaagta ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc aggtgatgct
    16741 gggttactgg cagcgtccgg atgctacaga tgagatcatc aaaaatggct ggttacacac
    16801 cggcgacatc gcggtgatgg atgaagaagg attcctgcgc attgtcgatc gtaaaaaaga
    16861 catgattttg gtttccggtt ttaacgttta tcccaacgag attgaagatg tcgtcatgca
    16921 gcatcctggc gtacaggaag tcgcggctgt tggcgtacct tccggctcca gtggtgaagc
    16981 ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac tggtgacttt
    17041 ttgccgccgt cagctcacgg gatacaaagt accgaagctg gtggagtttc gtgatgagtt
    17101 accgaaatct aacgtcggaa aaattttgcg acgagaatta cgtgacgaag cgcgcggcaa
    17161 agtggacaat aaagcctgag cgttaagtca gtcgtcagac gccggttaat ccggcgtttt
    17221 ttttgacgcc cactaaagag aaaacaattt gaattaccaa atgattacca cggacgatgc
    17281 gctggcttct ttgtgtgaag ccgtccgtgt ctttccggcg atagccctgg atactgaatt
    17341 tgttcgtacg cgcacttatt acccgcagct ggggttgatt caacttttcg atggcgagca
    17401 tctggcgcta attgatcctc tcgggatcac cgactggtca ccgctgaaag cgatcctgcg
    17461 cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa gatctggaag tgttcctcaa
    17521 tgtctttggc gaattaccac aacccttgat tgacacgcaa atccttgctg ccttctgcgg
    17581 acgcccgatg tcatggggtt tcgcttccat ggtggaagag tattccggcg ttacgctgga
    17641 caagagtgaa tcgcgcaccg actggctggc cagaccgctg accgaacgtc agtgtgaata
    17701 cgcagcggcg gatgtctggt atctgttacc gatcaccgcc aagcttatgg tagaaacgga
    17761 ggcctccggc tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa tgcgtcgtca
    17821 ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc aatgcctggc agttacgcac
    17881 acgccaactg gcctgtctgc aactgttagc cgactggcga ctgcgcaagg cgcgagagcg
    17941 tgatctggcg gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg cgcgttatat
    18001 gccgggaagt ttaggcgaac tggacagcct gggtttatcc ggtagcgaaa tccgctttca
    18061 cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag atgccttacc
    18121 gcagccgatg cttaacctga tggacatgcc tggttatcgt aaagcgttta aagcgattaa
    18181 gtcgctgatt actgacgtga gcgaaacgca taagatcagc gccgaattgc tggcatcgcg
    18241 tcggcaaatc aaccaactgc tgaactggca ctggaaactg aaaccgcaga acaatttgcc
    18301 ggagctgatt tccggctggc gtggtgagct gatggcggaa gcattacaca atttattgca
    18361 ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc cacatccggc
    18421 tttggcaaac tacaaatcca acaccaggcg tttacccttc gcacgcgaac aacagatcaa
    18481 catactttgc tggctggcac gctcttcatc gctaaaatac tgatcccgat ggtcagcttc
    18541 accttccagt attgccgttt cgcaggttcc gcatacccct tcacgacata aacattccac
    18601 tttcgcggct ttattatttt caataacctg caaaatagtc atctcttccg gcaccacaaa
    18661 ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg ccggttttgt cttcgatagc
    18721 aaattgctca aagtgcagtg tatcggcggc gatgtccaga cgcgcagctt cacttcttac
    18781 cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt gtgccaggtt cgatatccgc
    18841 caataatcgc gctaattcca gtcgtgttcc ggttgatgaa tgatgcaaat ggactttctc
    18901 agcctgcgga tgctggacta gctcagcacg aaatgcgcaa ctttctggat ttcgcgagca
    18961 gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct gccatgtgcg acaggaaagg
    19021 ggtgatacca ataccgccca cgataaacag atgctttctg gctgagggaa ttagcgcaaa
    19081 caggttatta ggcgttgaga tcgttaaccg atcgcccact tttacctgct gatgcaaaaa
    19141 gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca atctgataac tagaggtgtt
    19201 atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg ttatcaccat cgctcatctg
    19261 cactaggatg tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg ttgccaccag
    19321 cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg ctcacctgta cttcaaacat
    19381 ttgatagtct gacattgctg tactccatca aaaatcagcc cagcggcgga aaataccgcc
    19441 gccgatgtca ttagtcctta aacacctgcg ccagcagatt atggaaatgg gcgataccat
    19501 gttcggaaat gccactaccg ctactgtcgg ccatgatgcg cccctgacca cgatagccac
    19561 gcgatttcag ccctttctgt acgctttcaa ccagacgtaa atcttccgga tggaacacat
    19621 cgcgatacca ctcaatcagc gatttttgct cgtcggttaa ctcttcattg gtgaagtaaa
    19681 tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa ttcataaatg accgtcatca
    19741 tccctttgat cggggtgacg ttcagcatcg tgcacggcca cagccagaaa ccgtggaatg
    19801 ccgcatccgt accctcttca aatttaaacg actgttcgga cggtttggcg aaaccgtatt
    19861 gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg gagtcggaga
    19921 aacctagatg cgccggacca cagtgatagc actcgagata gttatcgaca atgttcttcc
    19981 agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt cagattgtgg acttccgggc
    20041 aggcttccag cactttcgcg cccagaccgg tgtattgatt cacttgaagt acgaaaaaaa
    20101 accgggagga cattggatta ttcgggttct gatgggatta gatttggtgg ggcttgcaag
    20161 cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga aagatagtaa aaaattgctt
    20221 ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat catttcaata acagcttcaa
    20281 aaaacgttca aaaccgataa caaccaggct gtaaccaaat gactcatatc acaaatcagc
    20341 tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg tgaagtatgt tttagctggt
    20401 tcatggttgt tatacggctt tttttacatc ttgtggttct tgtgaaggag ctacaagacc
    20461 ttcctgttca taaataccat actttgacaa aatcttttta cgagtatctg ttggtaatga
    20521 ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata ccttgcaaat cagcctggat
    20581 ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca aatcctttct ttctgaacaa
    20641 ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg acaattgcga tcagtttagt
    20701 tttcaatgtg ctgcggtcag gttgagaatt tttattctct tgattatttt tatgctcaac
    20761 cattggcatg ctctgacttt ggataaacca ctctattgga cgccctgttg cgtaggctat
    20821 ttgtgctaat cgatctaaag acgggtatgt cttgccagac aggtagtttc tgataacccc
    20881 atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat tctccattca ttgcctcttc
    20941 aaggcgttcc ttgaaacgca ttatttccgg gggcataaaa atgcgctctg catttttgct
    21001 ttctttttca cctgtcatgc ccaatctctc tgttatataa agaattttac agaattcttt
    21061 gatttgcact ggttaaaata aaaatgcgca tagcttttga attgagcatt tttctgcgct
    21121 atcgtttttc ccgatagcgg tagccggtat cagttaccga aaacgttaag agagtaagga
    21181 tcgcagaatg tcagcaaaaa accaagactg gcacgcagag cagataaagg ctgctgttcg
    21241 tatgaagggg aagacactat ctcaattatc cagag
//
LOCUS       DAZOYF010000060        20714 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZOYF010000060 DAZOYF010000000
VERSION     DAZOYF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.060"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..145)
                     /locus_tag="ACHUOF_002105"
     CDS             complement(<1..145)
                     /locus_tag="ACHUOF_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0611284.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNK"
     gene            complement(323..1972)
                     /gene="actP"
                     /locus_tag="ACHUOF_002106"
     CDS             complement(323..1972)
                     /gene="actP"
                     /locus_tag="ACHUOF_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR0611285.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1969..2283)
                     /locus_tag="ACHUOF_002107"
     CDS             complement(1969..2283)
                     /locus_tag="ACHUOF_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFR0611286.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2483..4441)
                     /gene="acs"
                     /locus_tag="ACHUOF_002108"
                     /pseudo
     CDS             complement(2483..4441)
                     /gene="acs"
                     /locus_tag="ACHUOF_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4834..6270
                     /gene="nrfA"
                     /locus_tag="ACHUOF_002109"
     CDS             4834..6270
                     /gene="nrfA"
                     /locus_tag="ACHUOF_002109"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR0611287.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6315..6881
                     /gene="nrfB"
                     /locus_tag="ACHUOF_002110"
     CDS             6315..6881
                     /gene="nrfB"
                     /locus_tag="ACHUOF_002110"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR0611288.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6878..7549
                     /gene="nrfC"
                     /locus_tag="ACHUOF_002111"
     CDS             6878..7549
                     /gene="nrfC"
                     /locus_tag="ACHUOF_002111"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR0611289.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7546..8502
                     /gene="nrfD"
                     /locus_tag="ACHUOF_002112"
     CDS             7546..8502
                     /gene="nrfD"
                     /locus_tag="ACHUOF_002112"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR0611290.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8522..10240
                     /locus_tag="ACHUOF_002113"
     CDS             8522..10240
                     /locus_tag="ACHUOF_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR0611291.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQENSLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10233..10616
                     /gene="nrfF"
                     /locus_tag="ACHUOF_002114"
     CDS             10233..10616
                     /gene="nrfF"
                     /locus_tag="ACHUOF_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR0611292.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10613..11209
                     /gene="nrfG"
                     /locus_tag="ACHUOF_002115"
     CDS             10613..11209
                     /gene="nrfG"
                     /locus_tag="ACHUOF_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR0611293.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11551..12864
                     /gene="gltP"
                     /locus_tag="ACHUOF_002116"
     CDS             11551..12864
                     /gene="gltP"
                     /locus_tag="ACHUOF_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR0611294.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12942..13631)
                     /gene="yjcO"
                     /locus_tag="ACHUOF_002117"
     CDS             complement(12942..13631)
                     /gene="yjcO"
                     /locus_tag="ACHUOF_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR0611295.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13725..15872)
                     /gene="fdhF"
                     /locus_tag="ACHUOF_002118"
                     /pseudo
     CDS             complement(13725..15872)
                     /gene="fdhF"
                     /locus_tag="ACHUOF_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(16070..17536)
                     /gene="mdtP"
                     /locus_tag="ACHUOF_002119"
     CDS             complement(16070..17536)
                     /gene="mdtP"
                     /locus_tag="ACHUOF_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR0611296.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17533..19575)
                     /gene="mdtO"
                     /locus_tag="ACHUOF_002120"
     CDS             complement(17533..19575)
                     /gene="mdtO"
                     /locus_tag="ACHUOF_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR0611297.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            complement(19575..20606)
                     /gene="mdtN"
                     /locus_tag="ACHUOF_002121"
     CDS             complement(19575..20606)
                     /gene="mdtN"
                     /locus_tag="ACHUOF_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR0611298.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20625..>20714)
                     /locus_tag="ACHUOF_002122"
     CDS             complement(20625..>20714)
                     /locus_tag="ACHUOF_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611299.1"
                     /translation="RANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4648 a         5460 c         5667 g         4939 t
ORIGIN      
        1 ctttattgtc aattaacgtg tttttatgat gtttgctgta attatctgat tttgttttct
       61 ttgtatgtga tgaacttgtg atagagtggt tatcgttaat ctggcaaaga gggaaaaaca
      121 tattatttaa accattatag cgcataaaat atccttataa ttaacaagaa agaaaaggca
      181 tattctctgc attatcattt tctacgactg tcaaaaatcg ctcatttttt aatgagttta
      241 tttgtttaat attatgggaa aaggtgatgc atgttggaga ggaagcgtat tccccggtca
      301 ggcgaccggg gaaggggaga gattaatgtg cgcggccttg ctcaacgcca aagccggtct
      361 gggagcggat aaactgcgcg cggaacagtt cacgctcgcg cgccccttcc gctgagttat
      421 cggttgccga gaagaaccag atgccgagga atgccacgct gatagagaac agcgccgggt
      481 attcatacgg gaagatggct ttttcgtgac cgaggatctg tacccaaata gtcgggccga
      541 ggatcatcag caccactgcg gtaatcagcc ccagccagcc gcccagcatc gcgccacggg
      601 tagtcagttt cgaccagtac atggaaagca gaatgatcgg gaagttacag ctcgccgcga
      661 tggcaaacgc aaggcctacc ataaaggcga tgttctgatt ctcaaacagc acgccgagga
      721 taatcgcaat cacgccgagg atcagtacgg tgatttttga tacccgcagc tcttcacgtt
      781 cggtcgcgcc ttttttgaag acgttagcgt acaagtcatg cgaaaccgcc gatgcgcccg
      841 ccagcgtcag atccgcaacc accgcgagga tagtggcgaa agcaaccgct gaaataaaac
      901 cgaggaatag gttgccgccc actgcattcg ccaggtgaac ggccgccatg ttgttaccac
      961 caatcagatg gcccgccgcg tctttatatt ccggattcgc accaacaagc atgatcgcgc
     1021 cgaagccgat aataaaggtc agaatataga agtagcccat aaatccggtg gcgtagaaca
     1081 cgctcttacg tgcttcgcgg gcatcgctga ctgtaaagaa gcgcatcaga atgtgcggca
     1141 agcccgccgt accaaacatc agtcccagcc ccagagagag cgcggagatc ggatctttca
     1201 ccagtccgcc cggtttcatg atgtcgacac ctttcgggtg taccgccatc gcttcgctga
     1261 acagattgtt gaagctaaag ccgacgtgtt tcatcaccat aaaggccata aagctggcac
     1321 cgaacagcaa cagcacagct ttgataattt gtacccaggt ggtcgccagc atgccgccga
     1381 acaggacgta catcatcatc agcacgccga ccagcaccac cgcaatgtga tagttaaggc
     1441 caaacagcag ctcgatcagt ttacctgcgc ctaccatctg agcgataagg taaagcgcca
     1501 ccactaccag agaaccacag gccgaaagaa tacgaatcgg cccttgtttc agacgataag
     1561 aggccacatc ggcagaggtg tagcgtccca ggttacgcag acgttcggca atcagaaaca
     1621 gaatgattgg ccagcccacc aggaagccca acgagtagat cagcccgtca tagccggagg
     1681 taaacaccag cgcggagatc cccaagaatg aggcggcgga catatagtcc ccggcaatcg
     1741 ccagcccgtt ctggaagcca gtgatattgc cgcctgcggt gtagtagtcg ctacgagaac
     1801 gtacgcgttt tgatgcccag taggtaatgc cgagcgtaaa cacgacgaaa atcaggaaca
     1861 taataatcgc ctgccagttc gttggctggc gctctacggc cccgctaata gcatccgcgg
     1921 cgttagctgc gaaagggagt gtggcggcaa gcgccgtcag aactcttttc atgatgcttg
     1981 tacctcatgc aggacttcat tattaagacg gtcgaattcg ccgttcgccc gccagatgta
     2041 gataccggtg agaacaaagg agatcacaat cactccaaca ccaattggaa taccgcgtgt
     2101 gacgctggtg ttcggattca gcggcgtgcc cagccagccg ggcgcgaagg cgatcagtaa
     2161 aataaagccg atataaactg ccagcataat aatcgacagg atggtggcaa accgttgccg
     2221 tttttcgact aactccctga aatgcgcatt gtcttctatc cgctgataaa tcgtaccatt
     2281 catcacagat tctccagagg taatgtaggg attgttttaa ttcccgctcc cttatgggag
     2341 aaggttaacg ctcgggtaac ccttgccgaa tgtaggccgg ataaggcgtt tacgccgcat
     2401 ccggcaatca atgcctgatg cgacgctgtc gcgtcttatc aggcctacaa accgttaccg
     2461 attcgcatcc ggcaattgtg ggttacgatg gcatcgcgat agcctgcttc tcttcaagca
     2521 gcttctcgac cacgccagga tcggcaagcg tcgaggtatc gcccaggttg ctggtatcgc
     2581 ccgccgcaat tttgcgcaga atacggcgca taattttgcc ggagcgggtt ttaggcaggg
     2641 agtcggtcca gtgcagcacg tctggcgtcg ccagcgggcc aatctcttta cgcacccagt
     2701 tgcggacttc tgcgtacagt tctggtgacg gttcctcccc gtgattaagc gtgacatagg
     2761 cgtagatcgc ctgaccttta atattgtgcg gaatacctac tacggcggct tcggcaatct
     2821 tcggatgcgc caccagcgcc gactctatct ctgccgtccc cagacggtga ccggagacgt
     2881 tcagcacgtc gtccacacgc ccggtgatcc agtaatagcc atcttcatcg cgacgcgcgc
     2941 cgtcgccgct gaaatacata tttttgaagg tggaaaaata ggtctgctca aaacgttcgt
     3001 gatcgccaaa cagcgtacgc gcctgaccag gccaggagtc ggtgatcacc aggctacctt
     3061 cggtagcccc ttccagcggg ttaccttcgt tatcgaccag cgccggttgc acgccgaaga
     3121 acggacgtgt tgccgaaccg gctttcagct cggtagcgcc aggcagcggg gtgatcatga
     3181 aaccgccggt ttcggtctgc caccaggtat cgaccaccgg acatttctcg ttgccgattt
     3241 ttttccagta ccactcccac gcttccgggt taattggctc gcccacggaa ccgagaatgc
     3301 gtagcgacga acggtcggtg ccttcaatcg ctttatcgcc ttccgccatc agcgcgcgga
     3361 tcgccgtggg tgcggtatag agaatattga cctgatgctt gtccaccacc tgcgccatac
     3421 gggcaggcgt cggccagttc ggtacgcctt caaacatcag cgtggttgcg ccgcaggcca
     3481 gcgggccgta cagcaaataa ctgtgtccgg taacccagcc cacatcggcg gtgcaccagt
     3541 agatatcacc cggatgataa tcaaagacat atttaaaggt cagcgccgcg tacaccagat
     3601 aaccgccggt agtgtgcagt acgccttttg gttttccggt agaaccggag gtatagagaa
     3661 taaacagcgg atcttcggcg ttcatctctt ccgcctagtg ctgatcgctg gcttgctcaa
     3721 ccaggtcgtg ccaccacagg tcgcgccctt cctgccagtc gatgtttccg ccagtacgct
     3781 tcagtaccac cacatgctct acgctggtga cgttcgggtt ttttagtgcg tcatcaacgt
     3841 ttttcttcag cggaatacta cgcccggcgc gcacgccttc gtcggaagtg atcaccagtc
     3901 gtgagttgga atcaataatg cgcccggcaa cggcttccgg cgagaagccg ccgaaaatca
     3961 ccgaatgcat cgcgccaatg cgggcgcagg ccagcatcgc aaccgcggct tccggcacca
     4021 tcggcatata aatcgccacc acatcacctt ttttaatgcc cagctcgagc agggtattgg
     4081 cgaagcggca gacgtcgcgg tgcagctctt tatagctgat atgtttgctc tggctggcgt
     4141 cgtcgccttc ccagatgatg gcggtgcgat cgccgttttc ttgcagatgg cggtcaaggc
     4201 agtttgccgc cagattcagc gtgccgtcct cgtaccattt aatggacaca ttaccggggg
     4261 caaaggaggt atttttcacc ttctggtacg gtttgatcca gtcgagaatt tttccctgtt
     4321 cgccccagaa ggtatcaggt acgttaatag attgttgata catcgcctcg tactgctgag
     4381 ggtttatcag gcaacggtct gcgatgttgg caggaatggt gtgtttgtga atttggctca
     4441 tgcttttgtt ctccttgtag gatgttaata atatgtggca taagcgttaa atgtaggggt
     4501 attggcagtt ttgtttagta tttgggcgac agatcacgca aaagtagaat ggtgcaaata
     4561 aacggcaggg taatttttga aggtcaccaa caaaagttga ttaattcttt gaggaacatg
     4621 cagttatgca tgctgttgaa aaagaggaag atactgacta actctaaagt ggtatttcac
     4681 atgcacttac aattgattaa agacaacatt cacagtgtgg ttatttgtta cacatagggg
     4741 tgagcaatgt catgacagtg taggtgcggt tactgtcgtg aaaaacaata aaaaccgcca
     4801 ttgcaacaat ggcgcaattc ggatgaagcc cctatgacaa ggataaaaat aaacgcacgc
     4861 cgtatcttca gcttattgat tccttttttc tttttcactt ctgttcacgc tgaacaaacg
     4921 gcagctcccg caaaacctgt aactgtggaa gcgaagaatg aaacctttgc cccgcagcat
     4981 cacgatcaat atctctcctg gaaagccacc tcggaacagt cagagcgtgt tgacgccctg
     5041 gcggaagatc cacggctggt gatcctgtgg gcggggtatc ccttctcgcg cgattacaac
     5101 aagcctcgtg gacatgcctt tgctgtgacc gatgtgcgtg aaaccctgcg taccggtgcg
     5161 ccgaaaaacg ctgaagatgg tccgctaccg atggcgtgct ggagttgtaa aagcccggat
     5221 gtggcgcgtc tgatccagaa agacggcgaa gatggctact tccacggtaa atgggcgcgc
     5281 ggcggcccgg aaatcgtcaa caacttaggt tgtgccgact gccataacac cgcctcacca
     5341 gagttcgcca aaggcaaacc ggagttaacc ctttcccgtc cgtatgcggc tcgcgcgatg
     5401 gaagccattg gtaaaccttt tgagaaagcc ggacgtttcg accagcaatc gatggtttgc
     5461 ggtcagtgcc atgtggagta ttacttcgac ggcaaaaaca aagcggttaa attcccgtgg
     5521 gatgacggca tgaaagtcga aaatatggag cagtattacg acaaaattgc cttctctgac
     5581 tggactaact ccctgtcgaa aacgccaatg ctgaaagcgc agcacccgga atatgaaacc
     5641 tggacagcgg gcattcacgg taaaaacaac gtgacctgta tcgactgcca tatgccaaaa
     5701 gtgcagaacg ccgaaggcaa actctacacc gaccataaaa ttggtaatcc gtttgataac
     5761 ttcgcccaga cttgtgcgaa ctgccatacc caggataaag ctgccttgca aaaagtggtc
     5821 gcggaacgta agcaatcgat taacgacctg aaaatcaagg ttgaagatca actggttcac
     5881 gctcacttcg aagcgaaagc ggcgctggat gcaggcgcga cggaagccga aatgaagccg
     5941 attcaggacg atatccgtca tgcccagtgg cgttgggatc tggcgattgc ttcccacggt
     6001 attcatatgc acgcaccgga agaaggtcta cggatgctcg gtacggcgat ggataaagcg
     6061 gcggatgcgc gcaccaaact ggcgcgcctg ctggcgacca aaggtatcac ccatgaaatc
     6121 cagatcccgg atatctcgac caaagagaaa gcccagcagg ccattggcct gaacatggaa
     6181 caaatcaagg ccgagaagca ggacttcatc aaaacggtga tcccgcagtg ggaagagcag
     6241 gcacgtaaaa acggtctgtt aagccaataa ccccgttccg cctcgcaagg ggcggaaaac
     6301 acaatggagt gaatatgagc gtattacgtt cgttattaac tgctggggtg ctggcgtcgg
     6361 gcctgttgtg gagcctgaac ggaattaccg ctacccctgc ggtgcaggca tctggtgatc
     6421 gctacgaagt tacccagcag cgtaacccgg atgccgcctg tctggactgt cataaaccag
     6481 ataccgaagg tatgcatggc aaacatgctt ccgttatcaa cccgaataac aaattgccgg
     6541 tcacctgcac caactgtcac ggccaagcat caccgcaaca ccgcgaaggg gtgaaagatg
     6601 tgatgcgctt taacgagccg atgtataagg tcggagagca gaacagcgtc tgtatgtcct
     6661 gtcatctgcc ggaacagttg caaaaagcgt tctggccgca cgatgtccac gtaaccaaag
     6721 tggcgtgcgc cagctgccat tccctgcatc cacagcaaga tacgatgcag acgttaagtg
     6781 acaaaggacg gattaagatt tgcgtcgatt gccacagcga tcagcgcacc aatccgaact
     6841 ttaacccggc gtctgttccg ttgcttaagg agcagccatg acctggtctc gtcgccagtt
     6901 tctcaccggc gtcggcgtgc tggcagccgt cagcggcacc gcagggcgcg tggtggcgaa
     6961 aacgttgaat atcaatgggg tgcgttacgg catggtgcat gacgagtcgt tatgcatcgg
     7021 ctgtaccgcc tgtatggatg cttgtcggga agtgaacaag gtgccggaag gcgtctcgcg
     7081 cctgacgatt attcgtagcg agccgcaggg cgaatttcct gatgtgaaat atcgcttctt
     7141 ccgtaagtct tgccagcact gcgatcatgc gccgtgcgtt gacgtctgcc cgaccggtgc
     7201 gtcttttcgt gatgccgcta gcgggattgt cgatgttaat cctgatctct gcgtcggttg
     7261 tcagtactgc atcgccgcct gtccgtaccg cgtgcgcttt atccatccgg tcacgaagac
     7321 ggcggacaaa tgcgatttct gtcgtaagac caatttgcag gccggtaagc ttcccgcgtg
     7381 cgttgaagct tgcccgacga aggcgctgac gtttggcaat ctggacgatc ctaacagtga
     7441 gatttcgcaa ctgctgcgcc agaagcctac ttaccgctac aagctggcgc tgggaaccaa
     7501 accgaagctg taccgcgtac cgtttaaata cggggaggtg agccaatgac gcagacttcc
     7561 gcatttcatt ttgaatcgct ggtgtgggac tggccgattg ccatctacct gtttttgatt
     7621 ggtatttctg ccggtctggt gacgctggcc gtgctgttac gtcgcttcta cccgcaggcg
     7681 ggcggtgcag acagtacgtt gctgcgcacc acgctgattg tcgggccggg cgcggtgatc
     7741 ctcggtctgt tgatcctcgt cttccacctg acaagaccgt ggaccttctg gaagctgatg
     7801 ttccactaca gttttacctc ggtgatgtcg atgggggtga tgctgtttca gctctacatg
     7861 gtggtgctgg tgctgtggct ggcgaaaatc tttgaacatg atttgcttgc cctgcaacaa
     7921 cgctggttgc cgaagctggg gatcgtgcaa aaggttctga gcctgctgac gcccgttcat
     7981 cgcggactgg aaacgctgat gctggtgttg gcggtgctgc tgggggctta taccggcttt
     8041 ctgctgtcgg cgctgaaatc gtatccgttc ctcaataacc cgagcctgcc ggtgctgctc
     8101 ctcttctccg gcatctcgtc cggtgcggcg gtggcgctga tcgccatggc gatacgccaa
     8161 cgcagtaacc cgcattccac ggaagcgcac tttgttcacc ggatggaaat ccccgtggta
     8221 tggggtgaaa tcttcctgct ggtggcgttt tttgtcggtc tggcgctggg cgatgacggt
     8281 aaagttcgtg cgctggtggc ggcattaggt ggcggtttct ggacgtggtg gttctggctt
     8341 ggtgtcgccg ggctggggct gattgtgcca atgttgctca aaccgtgggt caatcgcagt
     8401 tccggcattc ctgccgtgct ggcggcgtgt ggggccagtc tggtcggcgt gttgatgctg
     8461 cgctttttca ttctctacgc cggacagttg acggtggcgt aagccagaaa agaggtggtt
     8521 tctggacgta ttccttcctg aagtcggttt tctggcgttg ttgttaagtc tcggggtcaa
     8581 cgtgttgacc ccgttgacgg cctttgcggg agtgcggttg cgctggcctg ccatgatgcg
     8641 actcacttgc atcggcattc tggcgcagtt cgcgctcctg ctgctcgcct ttggcgtact
     8701 gacgtattgt tttctcatca gcgatttctc ggtcatttat gtcgcccaac atagctacag
     8761 cctgctgtcg tgggaactca aactggcggc ggtgtggggc ggtcatgaag gttcgctgct
     8821 gctttgggtg ctgctgcttt ccgcctggag cgcgctgttt gcctggcatt atcggcagca
     8881 aagcgatccg ctatttccgc tgacgctagc cgttttatct ctcatgctcg ccgcactgct
     8941 actgtttgtg gtgctgtggt ccgatccctt cgtgcggata tttccaccag caatcgaagg
     9001 ccgcgatctc aatccgatgc tgcaacatcc cggtcttatc tttcatccac cgctgcttta
     9061 ccttggctat ggcggtttga tggtagcggc gagcgtggcg ctggcgagtc tactgcgcgg
     9121 cgagtttgat gcggccagcg cccgaatttg ctggcgctgg gcgttacctg gctggagtgc
     9181 attaacggcg gggatcatcc tcggttcctg gtgggcctac tgcgaactcg gctggggcgg
     9241 ctggtggttc tgggatccgg tggaaaacgc ctctttatta ccctggcttt ctgccactgc
     9301 gctgctgcac agtttgtccc tgacacgcca gcgggggatt ttccgccact ggtcgctgtt
     9361 gctggcgata gttactctga tgctgtcgct gctgggcacc ttaattgtcc gttctggcat
     9421 tctggtttcg gttcatgcgt tcgcactgga taacgtccgc gccgtgccgt tgttcagcct
     9481 gtttgcactg attagccttg cgtctttggc tctgtatggc tggcgagcgc gggacggtgg
     9541 cccggcggtg cgtttttcgg ggttatcgcg ggaaatgtta atcctcgcta cgctgttgct
     9601 gttttgcgca gtgctactga tcgtgctggt gggaacgctt tatccgatga tttacggcct
     9661 gctgggctgg ggacgcctct ccgttggcgc gccgtatttt aaccgcgcga cgttaccgtt
     9721 tggtctgttg atgctggtgg tgattgtgct ggcgacgttt gtctctggca aacgcgtgca
     9781 gcttccggcg ctggtagctc atgctggcgt gctgttattt gccgcgggga tcgtggtttc
     9841 cagtgtcagt cgtcaggaga acagcctgaa tttacagccg ggtcagcagg tgacgctggc
     9901 aggatacacc ttccgttttg agcgactcga tctgcaagcc agaggcaatt acaccagcga
     9961 aaaagcgata gtggcgctgt ttgaccatca gcaacgcatt ggtgaattaa cgccggagcg
    10021 gcgtttttat gaagcacgcc gtcagcaaat gatggaaccg tcaattcgct ggaacggcat
    10081 ccatgactgg tatgcggtca tgggtgaaaa aaccagagcg gatcgttacg cttttcgttt
    10141 gtatgtacaa agcggtgtgc gctggatctg ggggggagga ttgttgatga ttgcgggcgc
    10201 attgttaagc ggatggcggg ggaagaagcg cgatgaataa agggcttctc acgctactgc
    10261 tgttatttac ctgttttgcg cgcgctcagg ttgtagacac ctggcaattc gccaatccgc
    10321 aacaacagca acaggcgtta aatattgcca gccagttacg ttgtccgcag tgccagaatc
    10381 aaaacttact cgaatccaat gcgccggtgg cggtcagtat gcgccatcag gtttatagca
    10441 tggtgtcgga ggggaagaac gaagtcgaaa tcattggctg gatgaccgaa cgctacggag
    10501 attttgttcg ctataaccca ccgttaacgg gtcagacgct ggtgttgtgg gcgctgccag
    10561 tggtgttgtt attgctgatg gcactgatcc tctggcgagt gagggctaag cgatgaaaca
    10621 gcctcaaata ccggtgaaaa tgctgacaac actcacgata ttgatggtat tcctctgtgt
    10681 cggcagttat ctgttaagcc caaaatggca ggcggtacgt gcggagtatc agcgtcagcg
    10741 cgatccgcta catcagtttg ccagccagca aaccccggaa gcgcagcttc aggcattgca
    10801 ggataaaatc cgcgctaatc cacaaaacag cgaacaatgg gcgttactgg gcgagtacta
    10861 tctgtggcaa aacgattaca gcaattcgct gctggcgtat cgtcaggcgt tgcaactacg
    10921 tggtgagaac gctgaactgt atgcggcgct ggcgacggtg ctttattacc aggccagcca
    10981 gcatatgacc gcccagactc gcgcaatgat cgacaaagcc ctcgcgctgg actctaacga
    11041 aatcaccgct ctgatgctgc tggcttccga tgcgtttatg caggcgaatt acgcgcaagc
    11101 catcgaacta tggcaaaaag tgatggatct caactcaccc cggatcaacc ggacacagct
    11161 ggtcgagtcg attaatatgg caaaactgtt gcagcggaga tcggattaat catctggtga
    11221 tgaagattat gtcattgagc tgcataaaaa ataatcgaat gaacatatgc caaaaataat
    11281 caccaatcag tattattgca gattaaacaa ataaaaatct ttccataaca aatggttatt
    11341 cattaatcct gccataaact cgcgttatta tgcattaatg cagcgaaaag ctctgttgtt
    11401 aaagggttgc gcaacatacc gcgcaaatga tactgatcat aagcgttaaa aaaatctaca
    11461 aaccaacgca acacaattca tgccctggca gtatgtcacg ttctcgcgtt tctgaacggg
    11521 gaacggcgct ccattgagga agttattctg atgaaaaata taaaattcag cctggcctgg
    11581 cagattctgt ttgctatggt gctgggcatt ctcctgggaa gctacctgca ctaccatagc
    11641 gacagccgcg actggctggt cgtcaatttg ctctctccgg cgggtgatat cttcatccat
    11701 ctgatcaaaa tgattgttgt gccgattgtg atctccacgc tggtggtggg tatcgcgggt
    11761 gttggtgatg ccaaacagct cgggcgtatt ggcgcgaaaa ccattattta cttcgaggtg
    11821 atcaccaccg tcgccatcat tttggggatc actctggcga acgtcttcca gcccggtgcc
    11881 ggggtggata tgtcgcagct ggcgaccgtc gatatctcga aatatcagag cactacggaa
    11941 gcggtacaaa gcagttccca cggcattatg ggcacgattt tgtcgctggt gccgacgaac
    12001 attgtggcgt cgatggcgaa aggcgaaatg ctgccgatca tctttttctc ggtgctgttt
    12061 ggtctggggc tttcttccct gcccgcgacg catcgtgaac cgctggtgac cgtgttccgc
    12121 tccatctctg aaaccatgtt taaagtgact cacatggtga tgcgttatgc accggtgggt
    12181 gtgtttgcgc tgattgcggt gacggtggct aactttggtt tctcgtctct gtggccactg
    12241 gcgaaactgg tgctgctggt gcatttcgcc attctgttct tcgcgctggt agtgctggga
    12301 attgtggcac gtctgtgcgg gttaagcgtc tggatcctga ttcgtattct gaaagatgag
    12361 ctgattctgg cgtactccac tgccagctct gaaagcgtac tgccgcgaat tattgagaag
    12421 atggaagcct acggagcacc ggcgtcgatc accagcttcg tggtgccgac cggttactct
    12481 tttaaccttg atggttcgac gctgtatcaa agtattgccg ctattttcat cgctcagctg
    12541 tatggcattg acctgtccat ctggcaggaa atcattctgg tgctgacgct gatggtgacc
    12601 tcgaaaggga ttgctggcgt ggctggcgtg tcgtttgtgg tgttgctggc aacgctgggt
    12661 agcgtaggta ttccgctgga aggtctggcg tttattgctg gtgttgaccg tatcctcgac
    12721 atggcgcgta ctgcgctgaa cgtggtgggt aatgcgctgg cggtgctggt gattgccaag
    12781 tgggaacaca aatttgaccg caaaaaggcg ctggcttatg agcgtgaagt gctgggcaaa
    12841 tttgataaaa ctgcggatca ataattgaag attgccgggg atatcccccg gcaatgtgtg
    12901 gatgcctgat gcgacgcttg ccgcgtctta tcaggcctac cttaaccgtt ggttaatttt
    12961 tcaaactctt cacacccggt atcaaaccct tccatacagc tcaggttcaa ccagtgcagc
    13021 gccttttgtt tgttcttctc gataaaacct tcctcaccgt ttaagaacat cattcccgcc
    13081 cagtactcgg aataaccggt tcgggaaatt gcagagctgc gtttgaaata ccaggttgcc
    13141 ttgtcatcgt cggccttaat gcccacgccg ttggcgtaaa tcaaaccaag cagcatttgg
    13201 gcatcgacgg cggagtcatt ctccagatct tccgaggcgt tttctaacag cgaaatcgcc
    13261 tttggataat ccggtttacc cgcttgagta tttaccagaa tatgcgccag cgttacttca
    13321 ccttctttac tgcccgcttg cgccgctttc tctgcaagaa cttttgcttg tggatagtcc
    13381 agactgaccg gattggtgat tttgatctgc gccaacagcg cgcaggcatc agcatcaccg
    13441 ctgtcggcgg ctttctgtgc ccaatattcg gctttgctca aatcgccgga gctaaaccag
    13501 ctgtcggcaa gaaaatattg tgcacgtcgg tccccggcct ctgctgcctt taaatactgg
    13561 ctgccaggct cggagtcgtt cgcgtgggca aagaatgtca aaaacaacat caatgcgata
    13621 atttttttca ttttgataat acgattgggt agggagtaac cagtataaag agttcgaatg
    13681 gataaaaaaa cagcctccga aaggaggctg tagaaaggac tgtattacgc cagtgccgct
    13741 tcgcgcaggc gagttttcaa cttgttgtac tcgtcaatca cgtactgctc ggcggcgcgc
    13801 tgatcggaga tcggctcgac gcgcacggcg cagtatttgt attccggcgt tttcgtaatc
    13861 gggcttaagc tttcggtaac cagctcgtta caggcaccaa tccaccactg gtaggtcatg
    13921 taaatcgccc ctttgttcgg acgatcgctg acctgcgcac gggtgataat tttgccttta
    13981 cgcgagtgca ctcaaaccaa tgcctcatct tcaataccca gacgtttggc gtcttcggta
    14041 ttgatttgtg cgtagccagg ttcatcagcc agcgccgcca gtgccgcaca gttaccggtc
    14101 atcgaacggc aagagtagtg accaacttca cgcaccgttg acagtaccat cgggtactcg
    14161 tcggtgagtt tgtcgattgg cgctacccag tcgcaggtga agaactgcgc cagaccgttc
    14221 ggggtatcaa acttctcttt aaacagataa gaagtcccct gatcggcatc tgaagtatcg
    14281 cggcaaggcc actgaatgaa gcccagttcg cccattttct cgtaagtcgc accgtagaaa
    14341 tccgggcaca gatgacgcaa ctcatcccag atctcctggg tgttgttgta gtgcatcgga
    14401 taacccatac gggtggcgat ttcactgatg atttgccagt ccgttttcag atcccatttc
    14461 ggttcaactg ctttgaagaa acgctggaag ccacggtcag ccgcagtaaa cacgccttca
    14521 tgctcgcccc acgacgttga cggcaaaatg acatccgccg ccgacgcggt tttggttata
    14581 aagatgtcct gaacgatgac cagttccaga tcttcaaagg ctttacgtac tgccgacagc
    14641 tccgcatcag tttgcagcgg atcttcgccc ataatgtacg cggcacgcac ttcgccatgc
    14701 gctgcgcggt gcggcagctc gctgatgcga tagccggtat gagcaggcag gctttccacg
    14761 ccccaggctt tggcgaattt ttcgcggtta gccggatctt tcacgtactg gtatcccgga
    14821 tacgtatccg gcagcgcgcc catatcgcag gcaccctgca cgttgttctg accacgaacc
    14881 gggtttacac ccgcatgcgg cttaccgagg ttactggtca gcatcgcgag gctggtcaga
    14941 gaacgcacgg tttccacgcc ctggtagaac tgggttacac ccatgcccca caggatggcg
    15001 gcgcttttcg cctgggcata catccgtgcc gcctgacgaa tctcactggc gctgacaccg
    15061 gtgatatctt caaccgactc cggcgtgtag ccttcaacga ttttacgata ctcttcaaag
    15121 ccttctgtac gggaagcgac gaacgctttg tcgtacagat tttcttcaat aatgacatgg
    15181 cccatcgcat tcaacagcgc gatgttcgag ccgtttttca gtgcaatgtg catgtcagca
    15241 atgcgcgcgg tttcaatttt gcgcggatcg cagacgataa ttttcgcccc gttacgttta
    15301 gcgttaatta cgtgattcgc cacgattggg tgggaatccg ccgggttgta cccgaaaacg
    15361 aacactaaat cggtattatc aatttcgtta atagcattgc tcattgcgcc attaccgacc
    15421 gattggtgca gacctgcaac cgatgggccg tgtcagacac gagcgcagca gtcaacgtta
    15481 ttggtaccaa taacggcgcg cgcaaatttt tgcattacat agttggtttc gttacccgta
    15541 ccacgcgagg agccggtcgt ctggatggca tccggaccgt acttctcttt gatggcgctc
    15601 aggcgctcgg caacgtaatt cagtgcctca tctcaggaaa caggttcgag tttgccgcca
    15661 cgctggcgac ggatcatggg ggttttcagg cgcggggtca ggatctgggt atcgttaatg
    15721 aaatcccagc cataataacc cttcagacac agggtaccct ggttggtttt cccctgcgct
    15781 gcctccgccc ggacgatttt gccgttatcg acgaccaggt tgattttgca acctgatgcg
    15841 caataggggc aaaccgtgac gacttttttc atcggtctcg ctccagttaa tcaaatcacg
    15901 catacgcgct ctcgactaca gtatgcatct tttatgccac attttatttg gggtcattcc
    15961 ctgatattac gggcactatt tattcaaaac tctgacgaaa aacaggctgt cgtcagtttt
    16021 gacgtgacga aacgaaatac cgcgtgacag ccatcacgcg gcagacattt tattttttct
    16081 cgacgacggg agccgcctga tacccgccgc ccagcgattt catcaactga atgctttgga
    16141 tcacccggcg gctgtccagc atcagtaatg acatctcttc ggcaagtact ggcaaccggg
    16201 cttcagtggc ctgtaagcgg ctggttaagc cgcgctgata ggcggcctcg gcagcgcgct
    16261 gggtgaagcg cgttgcttcc acgtgttcag cctgcatttc tcgctcgtcg ttgagcgttt
    16321 gcagacgcgt gccgttgacg gcaacgtcac gcaccgcgtt cagtactgac tggttgtaac
    16381 gttcaatcat catgttgctg gcggcgcgcg tgccttcgag attggcattc aaccgtccac
    16441 cgtcaaacag cggcaatttc agacccggga tgaagttgaa ctggcgactg gtttttttga
    16501 ataaggtatc caggtggatg gcatcaaggc cgaaaaacgc tttgatatca aagctcggat
    16561 agaacaacgc ccgcgcggaa tccacctgat ctaatgacgc ctgaacatac cagcgcatgg
    16621 cttgcagatc cgggcgtctg gcgagcaact cataagagag tgtcgccgga atgccggtct
    16681 ggactcgtgg taatgccaca ggtttgatct ccggcatatc gctggctccc gcgccaatca
    16741 atgcgcgcag agattctctc gtttcggtga tttgcccttt gacggcagca atttgtttat
    16801 cgaccgccag aatctgtgcc cgcgcgccgt ggaaaggcac ttgtgcttcc agaccgtgcg
    16861 ccactttact ttggtgcgct ttcaccgcgt aatcaatcac atcgcgagtt tgttctaaca
    16921 gatcgagcat ctgatagctg gcctgcatac tgtaataaag ctgcgctacg cccgtggtca
    16981 gcgatagctc tactgctgcg gtttctgcca gcgcggcatt atgcgcgcca atggcggcgg
    17041 caaccgctga gcgatgcaca ccccacaaat caagatccag tccggcaaac aaacctactg
    17101 tggcttccgt atagtacggc ccgtccatcc ccagtgcggg cgcatccatc gcataagggc
    17161 ttaaaaagcc gttcgccgag acacgttggc ggttaagcat ccctaacgct gccacctgca
    17221 actgtgaacc ggcatctaac aaatcggcct gcgattgcgc tttttcttcc cgcagtttcg
    17281 cttcggcgag ggtgtgtgaa ccacttagcg tccgttggat cagggcatcc aactgcgggt
    17341 cattgagttg tttccaccac tgcgcctgcg gccagccgga gctggcaaga tgaatatcat
    17401 cggccagttt gatttgttcc ggtttgagct gttgatgagg cgcagaatcc ttacgtacca
    17461 gggcacagcc ggaaatcagc gtcgtgctgc cgagaatgct gcacaacagc agacgtgaaa
    17521 gttgacgatt gatcattgcg tggctccttg tgcctgttcc gtggcgggcg ttaatgccgg
    17581 ggctgtccag tctggcaagc gggcgaaaag ctggcagacg tgttgctcct gttttaataa
    17641 ggtgggcagg atggcctgcg aggtgggtgt ctcttcaagc gttatttgag gagaacggct
    17701 gagtgcggtt gccagaccgg cagcgtattt ctccagggcg tcggcaaact gacctgctct
    17761 gtccggctgt agtgcgttat ccagcgcctg cgccgaattc caggtggcat cccaggcgtg
    17821 aaggatatcg cggccctgac gaataaccgt ttgcgaacgt tcaatcagta aggcgcgttc
    17881 ttcgctgtcc agttgacgct ccagcgccac gcgttggcac atctcttcac aggcattaaa
    17941 cgccgcatgc agaccgatac gaatttgcag ataagtgcgc agagccgtga cttcctgctg
    18001 gcgtgggatc cgcattactt tacttaacat acccagcgcg ccagccagtt tttgcggcag
    18061 cgtgcgcgct tcactttcag gccagacaaa ggtgtaaatc atcgcggaca ccacggtacc
    18121 aatgatgata cccagggcgc gatcgcgaat ttccaccagg tcgtacactg ggccaaaaac
    18181 gttttcgagc gtggcgagcg cgaaggtgac caccatctgt gtgccgatat aagaagagcg
    18241 ttcagagctg gtggcaatcc atgcgcccaa caggaaaatc ggtgccagca caaacagcaa
    18301 ttcgacaata ttgtccagcc agggcatgac cagtagcgtg aataacagcg ccagaatcgc
    18361 gccgcaaaag gccccgccaa aacgcagcac catcttctgg tacgacgaac cgacatttgg
    18421 attagcgacg atcacgcagg tcagcataca ggtgtgaatg ccttcccagt ccacgccgct
    18481 gtaaaaggtg taacagatca aacaggcgag caatgttttt accgcgtagc gtatatagtc
    18541 tggattggta aaagcatcgg cgaccattga tggcggtttg gcggcgggcg ttggcggcgt
    18601 attcgggttc atctggccca gttgtaacaa cgtctggcag atattctcca ggttacattc
    18661 ccgtgccgcc accgcttcac tttcactgag ccgccagtcg ctttgccagc actgaccttc
    18721 agcaacggca tgctgcagct tgttgatttc tgaggctaat ttttgccgaa attcaataat
    18781 tgcctgagaa tcagcaaaag aggtgggatc gtagcgattc agcgtcgagt aaatgtaggt
    18841 taccgttgcc acgcagcttt gccaccatgc gctttgagtt cgccagttgg catcgtccgc
    18901 gagacaaaag acattcagtt tttgcagcgc cagcgcctct ctttcaatcc gcgtttcggg
    18961 tagcggtgcg aggctgtccg tcaggtggct aatggcatca tcaagccgat cattaagcgc
    19021 ttgatgcatt tgcgtaatgg cacgattggg aaaccacagc acgccgatta acgtcatcag
    19081 caaggttgga tagaggccaa caacgataca ccacagcgtt aagcgcacga ctacttccgg
    19141 atagtcgagc atggcgggga aggtttgccc gtaaatggcg acagcgaaaa agaccagtcc
    19201 cagccgatgg gtgcgcatca aaaacatgca gcccatcagg atcggcccgg cgatgatcaa
    19261 tcggatcaac ggttcgccgt atgaccattt gtagatcaaa aacaggctgc cgatctccag
    19321 caccgtggca accacaaaca agatcgccac aaatttggtg taaaacgcgt tcgactgaat
    19381 accgtaaaac agcactgcca gcgataacgc cacaaaaggg atctcaaaag tcatcgagat
    19441 cagaatcacc agcaggcagc ctacccagag ttgcacgatc tgcggcacgc ggccgggtcg
    19501 ccgctcgctt aactcttcat gaaagaacgc cagcagcctg accaccggta atggcaggga
    19561 gttgagcgcg ctcattattg cggctcaaga ttagcgactg ccgaagcgcc gatgcggaac
    19621 atttccgggt caggtttatc gaccatgatt ttgaccggaa aacgctgggc aacgcggacc
    19681 cagttaatag aacgagacac ttttggcagg ccgcccagca ccaggccgcc gtcatccggt
    19741 agcacgccgt agccaatcga atccacttta ccctcgaagg ttttgccgct gtcactcatc
    19801 aggcgaatcg ttgcgggtgt acctgagcga atatttttca gatcggtttc gcggaagttg
    19861 gcgatcacgt accagtgacg agtgtcgatg agggtaaaaa tagggcgcat ggcagaagca
    19921 aattgcccga cggaggtttt gagggaaatg atccggccat caaatggcgc gcgaacggtc
    19981 gtcatttcca gatgtagttt ggtcagggca atatccgctt cgaccgccgc acgttgcgcc
    20041 accagcgcat cgacgccgct gacggcgctg gcggctgact gcgcctgtaa caatacggca
    20101 ttaagatccg cttctgcggc gcgctgcgcc gttcttgcac ggtcaacgtc ttctgctgag
    20161 acaaaaccct ctttcagtaa tggctcggtg cggcgtaatg tatctgtggc ctgtttcgcg
    20221 gcggcacggg ctttttctac cgtggcatta accgagtcgg caccaaactg ttgggcgtca
    20281 acgctacgct gggtgagcat aatttgctta tccagcgccg cgagggaggc ttcggctttc
    20341 gccaggttgg cttcgtacgg gcgcgggtcg atgcggaaca gcaaatcgcc ctgtttcacc
    20401 gcctggttgt cggtgaccgc cagttctaca atgcgaccgc tgacttccgg caccacatca
    20461 atggtatctg ctgacgcgta agcgtcatta gttgatggcg cactgtctac gcgccagata
    20521 acgaaaacaa gggcaaccag cgccaacgca accactaaca gagcagggaa tttactgcga
    20581 ggtgcttttt tcggcgtact ttccatgagc gcgtctcaaa tagattagaa aaatgccagc
    20641 cagaacagca tggcgtagag agcaaaaagg gcggtataaa taattccgac aaatgccaga
    20701 ttgatatttg cccg
//
LOCUS       DAZOYF010000061        20708 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZOYF010000061 DAZOYF010000000
VERSION     DAZOYF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.061"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACHUOF_002123"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACHUOF_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR0611300.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACHUOF_002124"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACHUOF_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR0611301.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACHUOF_002125"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACHUOF_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR0611302.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACHUOF_002126"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACHUOF_002126"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR0611303.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="ACHUOF_002127"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="ACHUOF_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR0611304.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="ACHUOF_002128"
     CDS             complement(8217..9110)
                     /locus_tag="ACHUOF_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR0611305.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACHUOF_002129"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACHUOF_002129"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR0611306.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACHUOF_002130"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACHUOF_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR0611307.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACHUOF_002131"
     CDS             complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACHUOF_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR0611308.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACHUOF_002132"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACHUOF_002132"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR0611309.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACHUOF_002133"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACHUOF_002133"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR0611310.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="ACHUOF_002134"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="ACHUOF_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACHUOF_002135"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACHUOF_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR0611311.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACHUOF_002136"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACHUOF_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR0611312.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACHUOF_002137"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACHUOF_002137"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR0611313.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACHUOF_002138"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACHUOF_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR0611314.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4981 a         5726 c         5029 g         4972 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatcctca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaat
//
LOCUS       DAZOYF010000062        20691 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZOYF010000062 DAZOYF010000000
VERSION     DAZOYF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.062"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            81..350
                     /locus_tag="ACHUOF_002139"
     CDS             81..350
                     /locus_tag="ACHUOF_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFR0611315.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="ACHUOF_002140"
     CDS             344..709
                     /locus_tag="ACHUOF_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR0611316.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHUOF_002141"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHUOF_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR0611317.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="ACHUOF_002142"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="ACHUOF_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR0611318.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHUOF_002143"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHUOF_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR0611319.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="ACHUOF_002144"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="ACHUOF_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0611320.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHUOF_002145"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHUOF_002145"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR0611321.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHUOF_002146"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHUOF_002146"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR0611322.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="ACHUOF_002147"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="ACHUOF_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACHUOF_002148"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACHUOF_002148"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR0611323.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACHUOF_002149"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACHUOF_002149"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR0611324.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14849..16447
                     /gene="rtcR"
                     /locus_tag="ACHUOF_002150"
     CDS             14849..16447
                     /gene="rtcR"
                     /locus_tag="ACHUOF_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFR0611325.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16429..17187)
                     /locus_tag="ACHUOF_002151"
     CDS             complement(16429..17187)
                     /locus_tag="ACHUOF_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR0611326.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="ACHUOF_002152"
     CDS             complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="ACHUOF_002152"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR0611327.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="ACHUOF_002153"
     CDS             complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="ACHUOF_002153"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR0611328.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18595..20100
                     /gene="glpD"
                     /locus_tag="ACHUOF_002154"
     CDS             18595..20100
                     /gene="glpD"
                     /locus_tag="ACHUOF_002154"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR0611329.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANALMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20306..20587)
                     /locus_tag="ACHUOF_002155"
     CDS             complement(20306..20587)
                     /locus_tag="ACHUOF_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611330.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIS"
BASE COUNT         4888 a         5692 c         5217 g         4894 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggtt acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttatt gtttctaaaa
    14761 agattatatc tttatatccg taaacggaga tttcccgcaa agccgattta ccgataatga
    14821 aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg gctttgtcgg
    14881 taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc gtccgtcact
    14941 ctgtatatac cagcaagaat cgttggtcat cgatcgactg gaattattgc acgatgcccg
    15001 ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaacaga
    15061 agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    15121 cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact atttaattca
    15181 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg aagcgcgtta
    15241 tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc cgcgcggccc
    15301 aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg ccagccgctt
    15361 tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca cgcgtaaccc
    15421 ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc gcgcgccgat
    15481 tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca tcttcgagtt
    15541 aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg ccaccctgcg
    15601 cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcgcg
    15661 ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc ttgatgagat
    15721 tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag agaaaacctt
    15781 ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    15841 gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt acgcgcggat
    15901 caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata ttgaaccgaa
    15961 cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc gttttaacac
    16021 cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggcaacat ggcgcggtaa
    16081 ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta gcggacgcat
    16141 cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    16201 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    16261 tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt ctgctgccgg
    16321 acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg cggatcggct
    16381 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    16441 cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg gggcgtcggt
    16501 gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac ggccaaattt
    16561 tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc gcttggtacg
    16621 aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc tgatccccag
    16681 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    16741 gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt cttttaccat
    16801 caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt gattgagcag
    16861 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg agccattggg
    16921 gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg tggctttacg
    16981 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat gatggcgcag
    17041 gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg ggctgacgga
    17101 gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca gttcgataat
    17161 accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt cgttttcgcg
    17221 cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg atgtgtgctc
    17281 cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag atcagcgcaa
    17341 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc
    17401 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    17461 atttttgctg catatagccg cttaacaggg cgctgataag cgtaatgaca attagcttac
    17521 cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc aggttaaaga
    17581 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    17641 tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg ccgagaattt
    17701 gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc ggacctgcgc
    17761 gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg ccggtatggc
    17821 ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    17881 gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    17941 gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    18001 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    18061 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gtcgctgcca
    18121 ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    18181 cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    18241 gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    18301 ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    18361 cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc tctctttctt
    18421 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    18481 atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg attctttgct
    18541 aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg cagcatggaa
    18601 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    18661 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    18721 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    18781 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc gcatatcgcc
    18841 ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgc
    18901 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaactggt
    18961 ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt cgaatattct
    19021 gactgttggg tagacgacgc ccgtctggta ctcgccaacg ccctgatggt ggtgcgtaaa
    19081 ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa cggcctgtgg
    19141 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    19201 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca tctgccttcg
    19261 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt gcatacccaa
    19321 aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat cccgtggatg
    19381 gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc gaaagcagtg
    19441 aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca ctttaaaaag
    19501 cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    19561 gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca tgatgaaaac
    19621 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggca
    19681 gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc gtggacgaaa
    19741 gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc cgctcgcctg
    19801 cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacacgcg cacttacggc
    19861 agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg ggaagatttc
    19921 ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg ggtccgccgc
    19981 accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    20041 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    20101 attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga caaacacaaa
    20161 actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat tgaatttgca
    20221 tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa gcgcactttg
    20281 tcaaaaatct aacctacttt ttaattcagg aaattaccgc aaagcccacg ccatcatacc
    20341 caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc attaatgcgc
    20401 ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag agtaaattgc
    20461 aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct gcaaaaaatt
    20521 gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc cattttctat
    20581 tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc
    20641 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg g
//
LOCUS       DAZOYF010000063        20523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZOYF010000063 DAZOYF010000000
VERSION     DAZOYF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.063"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="ACHUOF_002156"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="ACHUOF_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR0611331.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    689..814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            690..737
                     /gene="pheL"
                     /locus_tag="ACHUOF_002157"
     CDS             690..737
                     /gene="pheL"
                     /locus_tag="ACHUOF_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HFR0611332.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            836..1996
                     /gene="pheA"
                     /locus_tag="ACHUOF_002158"
     CDS             836..1996
                     /gene="pheA"
                     /locus_tag="ACHUOF_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR0611333.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="ACHUOF_002159"
     CDS             complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="ACHUOF_002159"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR0611334.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="ACHUOF_002160"
     CDS             complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="ACHUOF_002160"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR0611335.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4451..4816
                     /locus_tag="ACHUOF_002161"
     CDS             4451..4816
                     /locus_tag="ACHUOF_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR0611336.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4966..5484
                     /locus_tag="ACHUOF_002162"
     CDS             4966..5484
                     /locus_tag="ACHUOF_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR0611337.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5474..6699
                     /gene="dgcN"
                     /locus_tag="ACHUOF_002163"
                     /pseudo
     CDS             5474..6699
                     /gene="dgcN"
                     /locus_tag="ACHUOF_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6715..7197
                     /locus_tag="ACHUOF_002164"
     CDS             6715..7197
                     /locus_tag="ACHUOF_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR0611338.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="ACHUOF_002165"
     CDS             complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="ACHUOF_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR0611339.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="ACHUOF_002166"
     CDS             complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="ACHUOF_002166"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR0611340.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="ACHUOF_002167"
     CDS             complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="ACHUOF_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR0611341.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="ACHUOF_002168"
     CDS             complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="ACHUOF_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR0611342.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="ACHUOF_002169"
     CDS             complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="ACHUOF_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR0611343.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10941..11732
                     /locus_tag="ACHUOF_002170"
     CDS             10941..11732
                     /locus_tag="ACHUOF_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR0611344.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11753..13039
                     /locus_tag="ACHUOF_002171"
     CDS             11753..13039
                     /locus_tag="ACHUOF_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR0611345.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="ACHUOF_002172"
     CDS             complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="ACHUOF_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR0611346.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13810..14688
                     /gene="nadK"
                     /locus_tag="ACHUOF_002173"
     CDS             13810..14688
                     /gene="nadK"
                     /locus_tag="ACHUOF_002173"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR0611347.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14774..16435
                     /gene="recN"
                     /locus_tag="ACHUOF_002174"
     CDS             14774..16435
                     /gene="recN"
                     /locus_tag="ACHUOF_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR0611348.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16584..16925
                     /gene="bamE"
                     /locus_tag="ACHUOF_002175"
     CDS             16584..16925
                     /gene="bamE"
                     /locus_tag="ACHUOF_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR0611349.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16987..17277)
                     /locus_tag="ACHUOF_002176"
     CDS             complement(16987..17277)
                     /locus_tag="ACHUOF_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR0611350.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="ACHUOF_002177"
     CDS             complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="ACHUOF_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR0611351.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17875..18357
                     /gene="smpB"
                     /locus_tag="ACHUOF_002178"
     CDS             17875..18357
                     /gene="smpB"
                     /locus_tag="ACHUOF_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR0611352.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18572..18934
                     /gene="ssrA"
                     /locus_tag="ACHUOF_002179"
     tmRNA           18572..18934
                     /gene="ssrA"
                     /locus_tag="ACHUOF_002179"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19139..20380
                     /locus_tag="ACHUOF_002180"
     CDS             19139..20380
                     /locus_tag="ACHUOF_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR0611353.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5300 a         5359 c         4815 g         5049 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
      661 ctgtactaaa gtcacttaag gaaacaaaca tgaaacacat accgtttttc ttcgcattct
      721 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
      781 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
      841 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
      901 agcgttactg gcagaacggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
      961 gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat taattacgct
     1021 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     1081 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     1141 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     1201 gtatgctgcc cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     1261 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     1321 ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     1381 cgagatgacg ttaactatcg accattgttt gttggtctcc ggcactactg atttatccac
     1441 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     1501 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     1561 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     1621 tttgcaggta ctggagcgta ttgaagcaaa tcagcgacaa aacttcaccc gatttgtggt
     1681 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacca cgttgttaat
     1741 ggcgaccggg caacaagccg gtgcgttggt tgaagcgttg ctggtactgc gcaaccacaa
     1801 tctgattatg acccgtctgg aatcacgccc gattcacggt aatccatggg aagagatgtt
     1861 ctatctggat attcaggcta atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     1921 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     1981 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggca ctggattatt
     2041 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     2101 cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc tgcttatcgc
     2161 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     2221 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     2281 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     2341 gctgaacatt ttcttctgcc aggtgcagcc cgtaagcaaa agtagcaaag tggcgcagag
     2401 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     2461 cccagacctg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     2521 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     2581 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     2641 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     2701 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     2761 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     2821 acaggcgtcc catctgaccg ccaccgccga cgataaccac cggacgcagt gacggacaaa
     2881 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     2941 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     3001 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcaccttgc
     3061 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     3121 catcaatttg atcgcgtaat gcggtcaatt cagcaaccat aataaacctc ttaagccacg
     3181 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     3241 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     3301 tcagaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     3361 ttaccatctt tgatttgagc aaccacggat tctgccaccg caggctgacg gcgataatct
     3421 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     3481 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     3541 aggatcacat gcccgtccgg attcccctga gtttgtagca acgcaacctg ccctgcctgg
     3601 ttaatgccaa caaaacagtg cggctgggcg gcggcgcgca tagcgttaat tgctgttgcc
     3661 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     3721 cggtgagttt gcgattccgt tgtacgagca ccaattgctg accagctaaa cagatcgccc
     3781 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     3841 agctcaagca acaatttacg cgcgatttgc agcccggctt ctacatcaaa agagccatcc
     3901 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     3961 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagagcttt aaatcgacga
     4021 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     4081 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     4141 tcttgttgca ggctcaatgg aaaagcggcc ttcagttgtt ccggagtcat taaaacctgt
     4201 tcgtcggtaa tatgtacgtt attcagcgcg tctttatgca tgatggcgat cctgtttatg
     4261 ctcgtttgcg atagttgctc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     4321 tttcaatcca tatttcgtac atttttattt acacaggcaa tttagtcgcg ctttcaaccc
     4381 ttgcttctgt atagataaat ttacatcccc tttaaaaaca atccgctatg ctttgaaaaa
     4441 ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg ttagcggatg
     4501 tcagattgat ccttatactc acgcgccaac cttgaccagc accgactggt atgatgtcgg
     4561 tatggaagat gcgatatcgg gcagcgccat aaaagatgac gatgcattta gcgattcaca
     4621 ggcggatcgc ggtctatacc ttaaaggata tgccgaaggg caaaagaaaa cttgccagac
     4681 cgattttact tatgcccggg gactttccgg taaaagcttt cctgccagct gcaacaatgt
     4741 tgaaaatgcc agccaactgc atgaagtatg gcaaaaaagg gctgatgaaa atgccagcac
     4801 aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt tactcattgt
     4861 gttttatttc tcacattgat gacggtcgca tgaaaaaatg atattttaat gagcatcctg
     4921 aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg tttttctcac
     4981 cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc tcaagagcct
     5041 tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta cacccgctgg
     5101 cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt tagtaccgcc
     5161 ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca cactcaacaa
     5221 gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc gacattccag
     5281 atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga acaaaatacc
     5341 gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt cagatttgcc
     5401 gtaaacctgg atgcctcatc gcgtagcggt gtaaaggtca acccggatgt cttaatgctc
     5461 gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca cgtttaaaag
     5521 agcattacgc aacatcagta tgaccagcat atttatcact atgatgctga tctggttgct
     5581 gctttccgtg acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc tggcactgac
     5641 agcagcaaca atgacttaca gtctggaagc agctgtcgtt tttgccgatg gccctgcagc
     5701 aactgaaaca ctggcagcgc tgggccagca agggcatttt caactgcaga agtacgtgat
     5761 aagcagcaaa atattctggc atcctggcat tacccccgta aggatccagg cgatactttc
     5821 agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat tcgtcacaat
     5881 ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag ccattttatc
     5941 tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc
     6001 ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac cgatgtcgta
     6061 catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag
     6121 tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt
     6181 cgtttacagg ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc attaaccggg
     6241 ctggctaacc gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa ttccgatgcc
     6301 cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat caatgatacc
     6361 tggggtcatg cgacgggcga tagggtcttg attgaaatcg caaaacggtt agctgaattt
     6421 ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat
     6481 gatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca aatctttaat
     6541 ctcccgtttg atcttcataa tggccatcag accaccatga cattaagcat tggttacgcg
     6601 atgaccattg agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat
     6661 caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata
     6721 aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg ccagtcccct
     6781 cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc
     6841 gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa
     6901 ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc ctcgacaggg
     6961 ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa
     7021 ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctattgg tggacaaatt
     7081 ccacgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag
     7141 accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattgcaac cccttaaaac
     7201 acgagcaaac agacaaaaaa agggccagcc aattggccag cccttcttaa caggatgtcg
     7261 cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca gtacgctcac
     7321 gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa atgctgtcaa
     7381 ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa attttacgaa
     7441 cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc tcgaatgcct
     7501 gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta tcacccggac
     7561 ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata atgttgctca
     7621 taatttaatc tcttatcctg ggtaaactga tctctcgggg gcttacgcca tcccatcatg
     7681 tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg cttgctcttc
     7741 agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc ccagcgactg
     7801 tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg gcggaacttc
     7861 catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc cttcagcaaa
     7921 ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc gggaaacgga
     7981 gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc cgattgacca
     8041 ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc gaccgcacac
     8101 cagaatcaat ttttgattcg ttgccagttc gctgacgccc gcttgatcaa gcttgcgtcc
     8161 ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg ctgcatgaat
     8221 ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt aaggacgatc
     8281 gtccacggta cggtgccggt catgcgtgaa gtcgcgagga ctccaactct ggatgctcag
     8341 caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc ggaacatttc
     8401 aggaaacagg ctaattatgc caatccacat agcgccgcct tttaccgttt atccggtggt
     8461 ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga ctttcttgat
     8521 aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat ctttcaggtt
     8581 tgccttaatg acgagaacgt cattagatcc ggtttccatc atatcaacga ctttaccgag
     8641 atcgtagcct tcagtggtta ctacctggca gcccatcagg tctttccagt agtagtcccc
     8701 ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt tcacaattcg tcagcaggtt
     8761 cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat tgtggtgctt
     8821 ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc agggctgata
     8881 gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc cacgaatacc
     8941 gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg cggtgagttg
     9001 tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt tgatcagcgc
     9061 agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc gatccagatc
     9121 caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa cgcgctcgat
     9181 gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga acggacgctt
     9241 tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg tgaataaaac
     9301 aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa aattttactg
     9361 atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc atttagcgac
     9421 caggaaatcc cggcggcatc attcccttca tgcttctcat catcttcgcc attccgcctt
     9481 tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg cggttaacgt
     9541 cctgtacctg catcccgcaa ccggcggcga tacgacgctt acgcgaacct ttgatgattt
     9601 ctggcttagc gcgctctttc atcgtcattg agttgatgat ggcttccata cgcaccagca
     9661 ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc ggcagtttgc
     9721 ccatcaagct ggccatgccg cccatatttt tcatctggcg cagttgctca agaaagtcgt
     9781 tgagatcgaa gccgtcgcct ttttttagct tgctggctaa tttctctgcc tgcgcgcggt
     9841 caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg agaatacgcg
     9901 acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca acaccgagga
     9961 acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg cgggcatcgc
    10021 cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat gcttttgccg
    10081 tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca accgggttaa
    10141 tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc agacgaccag
    10201 cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc gccgcgttaa
    10261 caatatcaac cggcttctga ccaacatcag aagggaagaa atcaacgccc acctgctctg
    10321 ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcagcagaa accaccagca
    10381 ctttcttctt gtgcttctcg cgcaggaaat taccgagttt accaacgctg gttgttttac
    10441 cggcaccttg caggcctgcc atcagcacga ccgcaggcgg ttgcgcagcc agattcaggg
    10501 tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacgattttg acgaactcct
    10561 gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt acgcgattga
    10621 taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg cgcacttcgc
    10681 gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata ttgcgcagcg
    10741 tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg tggaaacggt
    10801 tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa ggcgtctttg
    10861 ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc tttcttattg
    10921 ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc gcctactccg
    10981 tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg cggcgcatgg
    11041 ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa gcccgcatcc
    11101 tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca ttggtcagtt
    11161 tggtgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg ttgctgctac
    11221 ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg cccaatgaat
    11281 acatcaccca tctggaagca acgcctggga tgctgatgca cattggctta tcgctctttt
    11341 cctatgccac gctaattatc gccgccctgt acgcgctgca actggcgtgg attgattacc
    11401 aactgaagaa caagaagctg gcatttaacc aagaaatgcc gccattgatg agtatcgagc
    11461 gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg ctttgcactg
    11521 gcctgttcta catgcacaac ctatttagca tggaaaatat cgacaaggct gtgctctcta
    11581 tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttaccatgaa ggatggcgtg
    11641 gacgccgcgt cgtctggttt aacgttgcgg gcgcggtaat tctgacactg gcctacttcg
    11701 gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc ccctggaaca
    11761 catttctact actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt
    11821 ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtcttcgac atatggcgaa
    11881 acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat
    11941 aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat
    12001 tgtcgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt
    12061 tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa
    12121 agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga tgccgctggt
    12181 ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat
    12241 cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcatg aatcgcgctc
    12301 acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg aaaaaatgac
    12361 cgttgatgac atcatggtac cgcgcagtga aattatcggt attgatatca acgatgactg
    12421 gaaatctatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tttaccgtga
    12481 ttcgctggac gacgccatca gtatgctgcg agtacgtgaa gcctggcggt tgatgtcgga
    12541 gaaaaaagag ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc
    12601 tgaaggtacg ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa agaaagtcgg
    12661 cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga
    12721 agagattgtt ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aggtcacgcc
    12781 gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa tcaacaaagc
    12841 ctttaactgg catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc
    12901 actggaagag atccctgtcg caggcacccg cgtgcgtatt ggcaagtacg atatcgatat
    12961 tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg
    13021 cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc gtgaattatt
    13081 acgaaaacag aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac
    13141 gaccattcag cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat
    13201 cagattccac cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg
    13261 cgatcacttc aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa
    13321 tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat
    13381 ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg
    13441 ctttttcaat atccagttca gtacgacgac gcaggttttc catttcggct tttacgcgca
    13501 aaataccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt
    13561 tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt
    13621 cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac
    13681 tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg
    13741 gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac
    13801 ctcggaaaaa tgaataatca tttcaagtgt attggcattg tgggacaccc acggcaccct
    13861 actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc
    13921 atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc
    13981 gcggagattg ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg
    14041 ggcgcggcgc gcacgctcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac
    14101 ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacaattagc cgatgtgctg
    14161 gaaggtcact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat
    14221 tgccaaaaac gcatcagcac cgcgattaac gaagtggtac ttcatcctgg caaagtggcg
    14281 catatgatag agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat
    14341 gggctaatta tttcgacgcc aacaggctcc accgcctatt ccctctctgc gggcggtcct
    14401 attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca
    14461 gcacgaccac tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt
    14521 aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga aggtgaagat
    14581 gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat
    14641 ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc
    14701 ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata
    14761 caggaaaacg actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct
    14821 tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggtg cgggtaaatc
    14881 tattgcaata gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg
    14941 taccggcgct gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc
    15001 cctgcgctgg ctggaagaaa accagcttga agacgggcat gaatgtttgc ttcgtcgcgt
    15061 gatcagcagt gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca
    15121 actgcgcgaa ctgggtcagt tgctgattca gatccatggt cagcacgctc atcaattact
    15181 caccaaacct gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact
    15241 tcaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca
    15301 tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc aattaaaaga
    15361 acttaacgaa tttaatccgc agcccggaga gtttgagcaa atcgacgaag agtacaaacg
    15421 tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga
    15481 cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt
    15541 gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca
    15601 gattgctgaa gccagcgacg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa
    15661 ccgactgttt gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca
    15721 cgtcagccct gaggcattac cacagtatta ccagtcgcta ctggaagaac agcagcaact
    15781 ggacgatcag gccgactcac aagaaacgct cgcgctggcg gtaacgaaac atcatcagca
    15841 ggcactggaa accgcgcgcg cattacacca acaacgccag caatatgcag aagaacttgc
    15901 acagctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgattga
    15961 tgtcaaatat gacgagcatc acctgggcgc tgacggtgcc gatcgtattg agtttcgggt
    16021 aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt
    16081 gtcccgcatc gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa ccccggcact
    16141 gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact
    16201 gctgcgtcaa ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc
    16261 gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac
    16321 acatatgcaa tctctggata aaaaagcgcg gttacaagag ctggcgcgcc tgcttggtgg
    16381 cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcag cgtaaacttt
    16441 tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt
    16501 ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg
    16561 gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca gcagcagtac
    16621 tattgatgtt aaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca
    16681 accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac
    16741 aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct
    16801 ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc
    16861 tgacctttaa cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta
    16921 actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc
    16981 tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc
    17041 aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc ttagttttgc
    17101 cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac gcaattccag
    17161 caagccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcactcgctg
    17221 caggtattgc ttctcaggta gcgcataagc cacctcaacg gcaattttac ctagcactgt
    17281 aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agccgccagc tctttaaaca
    17341 cgcgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga
    17401 tacgacacgc ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg
    17461 gcccatccac cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa
    17521 acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg
    17581 actccagaat ccgacttccg gtacaacctg gcaaaaactg aggataagac tgaacgtcat
    17641 tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag
    17701 gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct gctgtaaaaa
    17761 aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc ttgtcccccg
    17821 caggattgat atggggtatt ttcgatttca gattaccgat gattcacgac gcttatgacg
    17881 aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac
    17941 gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaagttaaa
    18001 tccctgcgcg caggaaaagc caatatcagc gacagctacg tccttctgcg tgacggagag
    18061 gcatttctgt ttggcgctaa catcacgcca atggccgtgg cctccacgca tgtggtgtgc
    18121 gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc attgtacggt
    18181 cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg
    18241 tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg ttcagatatc
    18301 aaagaacgcg aatggcaggt ggataaagca cgcatcatga aaaacgccca ccgttaaacc
    18361 tgcactccaa ttattgacca gttcctcacc gcgcctccct ctccggcggc gcgaatgaac
    18421 atcttattgg ctatcacatc cgacacaaat gttgccatcc cattgcttaa tcgaataaaa
    18481 atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatggcgct
    18541 cataaatctg gtatacttac ctttacacat tggggctgat tctggattcg acgggatttg
    18601 cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc
    18661 gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta
    18721 gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc
    18781 cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc
    18841 cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga tgtaggaatt
    18901 tcggacgcgg gttcaactcc cgccagctcc accaaaattc tccatcggtg attaccagag
    18961 tcatccgatg aagtcctaag agcccgcacg gcgcaagccc tgcgggcttt tttgtgctcc
    19021 gaatttgtcc cgcgaagtct gatgccaact aattaaatcc gatcctttta ggcaccttgt
    19081 taggcacttc ataaagctat attgtctttg aggtgcctaa aactatggaa acccggcaat
    19141 ggcaagacaa accaaacctc tatccgttaa ggaaatcgaa tctgctaaac ccaaggaagc
    19201 ggactacgtt ctctatgatg gcgatggcct tgagctactc attaaatcca gcgggagtaa
    19261 aatctggcag tttcgctaca ttcgccctgt caccaagaca cgagcaaaga aaagcatagg
    19321 cccttacccg tcagttaccc ttgccgatgc cagaaactac cgtgcagagt cccgctcact
    19381 cctagctaaa cagattgatc cgcaggaaca tcagcaagaa caattgcgta gttcgctgga
    19441 agccaaaacc aatactttcc agctcgtagc tgaacgttgg tggaatgtga agaaagccag
    19501 tgtgacagag gactatgcgg aagatatctg gcgctctctt gaaagagatg tctttcctgc
    19561 gattggcgac gttagcgtta cagatattaa agctcataca ctggttcagg ccgtacaacc
    19621 ggttcaggcc agaggagcac tggaaacggt tcgtcgcctg tgtcaacgta ttaatgaggt
    19681 catgatctat gcccagaaca cagggctgat tgatgctgtt cccagcgtta atatcggtaa
    19741 agccttcgaa aagcctcaga aaaagaacat gcccagcatt cgaccggatc agctatctca
    19801 actgatgcag acaatgcgaa cagccagcat tagcctttcc acacgctgcc tgttcatgtg
    19861 gcaacttctt actattaccc gccctgccga agcggctgaa gctcgctggg aagaagtaga
    19921 catagaagcg caagagtgga agattcccgc agcacgcatg aaaatgaacc gcgaccatac
    19981 tgttccattg tcagatgaag caatagctgt gctggagatg atgaaaccgc taagcggcaa
    20041 ccgagaattt atctttccta gccgtatcaa gcccaaccag cctatgaaca gtcagactgt
    20101 taacgcctca ctaaaacgcg caggttttgg tggtgtgctt gtttcgcatg gtctgagatc
    20161 aattgccagt acggctctta atgaacaagg ctttccgcct gatgccattg aggcagcatt
    20221 ggcccatgtg gataagaatg aggttcgtcg tgcttataac cgcagcgatt acctggaaca
    20281 gcgtcgcccg atgatgcaat ggtgggcaaa ttttgtgatg gcagcggatc gtggaagcat
    20341 gattgaaggt gggataaaag gaatgaagct agttgggtga ctaaatagct gcgcggcata
    20401 gtgtcccact ttcagccagc ccttgcaggt taagccacca gacagtaaac ggcgggtgtc
    20461 gcgttattag gcgcagctat ttagtagtaa tattaggatt ggtgattctg cactccagaa
    20521 gtt
//
LOCUS       DAZOYF010000064        20403 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZOYF010000064 DAZOYF010000000
VERSION     DAZOYF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.064"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="ACHUOF_002181"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="ACHUOF_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR0611354.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACHUOF_002182"
     CDS             complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACHUOF_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HFR0611355.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFVG"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHUOF_002183"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHUOF_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR0611356.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="ACHUOF_002184"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="ACHUOF_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="ACHUOF_002185"
     CDS             1631..2341
                     /locus_tag="ACHUOF_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR0611357.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHUOF_002186"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHUOF_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR0611358.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4129)
                     /locus_tag="ACHUOF_002187"
                     /pseudo
     CDS             complement(3469..4129)
                     /locus_tag="ACHUOF_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
     gene            4282..6480
                     /locus_tag="ACHUOF_002188"
     CDS             4282..6480
                     /locus_tag="ACHUOF_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFR0611359.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6449..6889)
                     /locus_tag="ACHUOF_002189"
     CDS             complement(6449..6889)
                     /locus_tag="ACHUOF_002189"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0611360.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACHUOF_002190"
     CDS             complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACHUOF_002190"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR0611361.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7607..8305
                     /locus_tag="ACHUOF_002191"
     CDS             7607..8305
                     /locus_tag="ACHUOF_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR0611362.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8534..9742
                     /locus_tag="ACHUOF_002192"
     CDS             8534..9742
                     /locus_tag="ACHUOF_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0611363.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10066..11757
                     /gene="eptB"
                     /locus_tag="ACHUOF_002193"
     CDS             10066..11757
                     /gene="eptB"
                     /locus_tag="ACHUOF_002193"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR0611364.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11849..11925
                     /locus_tag="ACHUOF_002194"
     tRNA            11849..11925
                     /locus_tag="ACHUOF_002194"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11883..11885,aa:Pro,seq:cgg)
     gene            12669..14276
                     /gene="dppA"
                     /locus_tag="ACHUOF_002195"
     CDS             12669..14276
                     /gene="dppA"
                     /locus_tag="ACHUOF_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR0611365.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14584..15603
                     /gene="dppB"
                     /locus_tag="ACHUOF_002196"
     CDS             14584..15603
                     /gene="dppB"
                     /locus_tag="ACHUOF_002196"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR0611366.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15613..16515
                     /gene="dppC"
                     /locus_tag="ACHUOF_002197"
     CDS             15613..16515
                     /gene="dppC"
                     /locus_tag="ACHUOF_002197"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR0611367.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16526..17509
                     /gene="dppD"
                     /locus_tag="ACHUOF_002198"
     CDS             16526..17509
                     /gene="dppD"
                     /locus_tag="ACHUOF_002198"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR0611368.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17506..18510
                     /gene="dppF"
                     /locus_tag="ACHUOF_002199"
     CDS             17506..18510
                     /gene="dppF"
                     /locus_tag="ACHUOF_002199"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR0611369.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18540..19811)
                     /locus_tag="ACHUOF_002200"
     CDS             complement(18540..19811)
                     /locus_tag="ACHUOF_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR0611370.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20287..20394
                     /locus_tag="ACHUOF_002201"
     CDS             20287..20394
                     /locus_tag="ACHUOF_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0611371.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4890 a         5049 c         5370 g         5094 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggcaggc
     4561 ggttataccg ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat
     4621 gtcgtaggtg gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat
     4681 agcgatgtcg tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat
     4741 gcatccgatg agcaggggct ttcttacttt tccgcactgc gtgacagtgg gaaaaagctg
     4801 atctgcattg atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg
     4861 gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg
     4921 gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc
     4981 gcctcttatt tgctgggcga gagtgacgga atagcgaaaa acgccgaatg ggcggcagag
     5041 atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc
     5101 accatgctga tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg
     5161 atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt
     5221 ctttcttacc attttgccaa tggtggtgac cccacgcggc gttctgcggt gctctcttcc
     5281 atgcagggca gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt
     5341 gttgaagcac tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc
     5401 ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc
     5461 aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg
     5521 acggcggcgg cgaaacacgc ggatattgtt ctgcctgcga ctacctcttt tgagcgtaat
     5581 gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg
     5641 ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttgag tgaacgttgg
     5701 gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg
     5761 ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct
     5821 gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt
     5881 attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc
     5941 aagattgaaa tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat
     6001 ccaatgtggc tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta
     6061 ctttctgccc atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa
     6121 ttgtacgcgg tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc
     6181 ggcataaccg aaggggatat ggtgcgggtg tggaactccc gtgggcagat ccttgccgga
     6241 gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg
     6301 gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa
     6361 gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacgacgct ggcatggctg
     6421 gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa
     6481 tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg
     6541 cacggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat
     6601 agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg
     6661 cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac
     6721 cgtcctcttc ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa
     6781 tgcagtcacg ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc
     6841 acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc
     6901 cagatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga
     6961 gtaacagatt gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc
     7021 gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg
     7081 ctgatgattt acaaacgacc agacaaagtc gggaaacggt tcgccgttct gttccatttg
     7141 caggtacgcc cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc
     7201 ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc
     7261 gaactgatga aagtaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa
     7321 cccagcctgc tgcccttcaa agcagatcat ttcgaacagt tttttactgt cagtttcagg
     7381 cacgccccac tcattatcat ggtaggcaat ataaagcggg ccctgactca cccagccgca
     7441 acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga
     7501 cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca
     7561 atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa
     7621 aagcggtggt cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct
     7681 caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac
     7741 agagcgttac acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg
     7801 tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt
     7861 ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac
     7921 cggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg
     7981 cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg
     8041 gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca
     8101 atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca
     8161 gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc
     8221 cgcctatgct gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac
     8281 gatgggggtt agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac
     8341 agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg
     8401 tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc
     8461 cccaagcata aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg
     8521 caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg
     8581 tactatcatt acccagtttg cgctggggtc ggtttatacc cggagcctgt ttaatggcgc
     8641 gctttctgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag
     8701 tctggggctg gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa
     8761 acgcgtcacc gtggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc
     8821 caacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc
     8881 gggttacctg ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat
     8941 ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac
     9001 gcagctgctc gaaacggtgg gtctggaaaa aacctttgtg atttggggag cgattgcgct
     9061 ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac
     9121 cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta
     9181 ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt
     9241 agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt
     9301 aacggttatt tccatcgcta acctttcagg tcgtctggtg ctgggcattc tgtctgacaa
     9361 aattgcccgt atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc
     9421 cctgctgttt gcaccattaa atgcagtgac gttctttgca gcgattgcct gcgtggcatt
     9481 taactttggc ggcactatta ccgtctttcc atcactggtc agtgagttct tcggcctcaa
     9541 taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttttgg
     9601 ttcgattatc gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct
     9661 gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg
     9721 tgaggcgcat ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg
     9781 catcaaacgc atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaactg
     9841 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
     9901 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
     9961 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
    10021 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
    10081 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
    10141 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
    10201 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
    10261 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
    10321 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
    10381 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
    10441 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
    10501 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggt ggtcgtcgta
    10561 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
    10621 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
    10681 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
    10741 gataataatt cattgcttaa tccggcgaag aagtttacat accaggctcc acaaaacgtt
    10801 gatgatactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
    10861 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
    10921 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
    10981 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg
    11041 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
    11101 aacacgatgg cagacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
    11161 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
    11221 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
    11281 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
    11341 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
    11401 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
    11461 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
    11521 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
    11581 aatgcgcagg cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
    11641 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
    11701 aacgaaaaca acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagcg
    11761 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
    11821 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
    11881 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
    11941 ctcaacgagc aggttttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
    12001 gaggctcgac tcgacgtagg cctgataaga cgcgccagcg tcgcatcagg caacggcacc
    12061 gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc aacacaccac acattttcct
    12121 gccaactttg cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc
    12181 tttttttata tttgcttaaa tcttttttgc gttacttatg gctggcgctg gcattttccg
    12241 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
    12301 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
    12361 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
    12421 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
    12481 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
    12541 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    12601 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    12661 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    12721 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    12781 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
    12841 tcccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg atcccgggcc
    12901 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
    12961 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg cgaaatgaac gccgatgacg
    13021 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg
    13081 gcagctacga atacttcgaa ggtatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13141 tggacgataa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13201 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    13261 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
    13321 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    13381 agatcgatac gctggtcttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    13441 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
    13501 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcgtata
    13561 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca agcactaacc tacgcggtga
    13621 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
    13681 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
    13741 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
    13801 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    13861 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    13921 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    13981 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    14041 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14101 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14161 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    14221 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    14281 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    14341 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    14401 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    14461 cctctgcgtg cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa
    14521 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg agtcactata gagaatccgg
    14581 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    14641 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    14701 ggcgaacgtg ggatctcccc tgagcgtcac gtgcagctgc tggctgaact cggcttagat
    14761 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    14821 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    14881 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    14941 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    15001 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    15061 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    15121 ccgttaaccg gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc
    15181 gatgccgtcg cccatatgat cttgcccgcc attgtgctgg gtactattcc gctagcggtc
    15241 attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc
    15301 gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    15361 atgctgccgg tagtgaccgt tatcggcctc caggtgggaa cattgctggc gggggcgatt
    15421 ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcactgcaa
    15481 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    15541 gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    15601 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    15661 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg
    15721 gtttacgtcg ccatcgtgct gttcatcgcg atctttgcca actggattgc gccttataac
    15781 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagt
    15841 atggcacact tgctgggcac cgatgacgtc ggtcgtgatg tgctgtcgcg gctgatgtac
    15901 ggcgcgcgtt tatcgctgct ggttggctgt ctggtggtgg tgttgtcgct gattatgggc
    15961 gttattctcg gcctgatcgc cggttacttc ggcggcctgg ttgataacat tatcatgcgc
    16021 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    16081 ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct tcgttgcctt gccgcactat
    16141 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt caccgcctct
    16201 cgcgtggcag gtgccggggc gatgcgccag atgtttatta acatcttccc gaactgcctt
    16261 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct
    16321 cttggtttcc tcggcatggg ggcacagccg ccaacgcctg agtggggcac catgctctcc
    16381 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttccctgg tctggcgatc
    16441 ctgttgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    16501 aaactaaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    16561 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    16621 gggcgaagtg gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc
    16681 gattatgggg ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg
    16741 ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    16801 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    16861 tatggaagcg attaaagtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat
    16921 tgacctgctg aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca
    16981 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    17041 aaaactgctg attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat
    17101 catcgaacta ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    17161 tgacctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggtcaggt
    17221 agtagaaacg ggcgatgcac acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    17281 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    17341 tgttgttccc ggcaagtatg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    17401 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca
    17461 gtccaaatgc cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag
    17521 gccacctcgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    17581 aaaggcatgt tcgtgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt
    17641 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    17701 cggttgctga cgatgatcga aacgcccacc ggcggcgagc tgtattatca ggggcaggat
    17761 ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    17821 cagaatccct acggttcgct gaatccgcgt aagaaagttg ggcaaattct cgaagagccg
    17881 ctgcttatta acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    17941 gcaaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcacatgtt ttccggcgga
    18001 caacgtcagc gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    18061 gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg
    18121 gatttgcagc aggagttggg gctttcttat gtctttatct cccacgacct gtcggttgtg
    18181 gagcacattg ctgatgaagt gatggtgatg tacctaggcc gctgcgtgga gaaaggaacg
    18241 aaggaccaaa tcttcaataa cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg
    18301 ccacgcctga acccggacga tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc
    18361 ccgctgaatc ctccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    18421 tgcacccagt tgcagccgca gctaaaagac tacggaggtc aactggtagc ttgttttgct
    18481 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    18541 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaatcagc
    18601 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    18661 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagcactgat
    18721 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    18781 agcatgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    18841 cctttaatag cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    18901 atattcagca ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagt
    18961 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    19021 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    19081 gtacgcagcg cgagccgggt tgccagtact ttatctgctt cccgtttacg ataggcaata
    19141 ttcattgggt taagtacctg aataaatacc gcagaaaaga agcaaaatgg aatggtaagc
    19201 aggacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    19261 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    19321 ccagaaatct taaacagtaa tcgttcacca ccagacgcaa tcgctatcag tacggcgaaa
    19381 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    19441 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    19501 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    19561 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    19621 actacccagg tgacaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    19681 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    19741 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    19801 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    19861 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    19921 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    19981 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    20041 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta gaatcagcgt
    20101 caagccggaa ggattccggt gaggcgcaat gttgcggggg cttaatccct ggtggcattg
    20161 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    20221 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    20281 ggctatatga cgctcgcaga gctgggcatg gccttctggc atgatttagc ggctccggtc
    20341 attgctggca ttcttgccag tatgatcgtg aactggctga acaagcggaa gtagcgtgtc
    20401 atg
//
LOCUS       DAZOYF010000065        20198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZOYF010000065 DAZOYF010000000
VERSION     DAZOYF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.065"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(37..2106)
                     /gene="glyS"
                     /locus_tag="ACHUOF_002202"
     CDS             complement(37..2106)
                     /gene="glyS"
                     /locus_tag="ACHUOF_002202"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR0611372.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="ACHUOF_002203"
     CDS             complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="ACHUOF_002203"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR0611373.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3122..3421)
                     /locus_tag="ACHUOF_002204"
     CDS             complement(3122..3421)
                     /locus_tag="ACHUOF_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR0611374.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3596..4591
                     /gene="wecH"
                     /locus_tag="ACHUOF_002205"
     CDS             3596..4591
                     /gene="wecH"
                     /locus_tag="ACHUOF_002205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR0611375.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4633..5070)
                     /gene="yiaA"
                     /locus_tag="ACHUOF_002206"
     CDS             complement(4633..5070)
                     /gene="yiaA"
                     /locus_tag="ACHUOF_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFR0611376.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5116..5457)
                     /gene="yiaB"
                     /locus_tag="ACHUOF_002207"
     CDS             complement(5116..5457)
                     /gene="yiaB"
                     /locus_tag="ACHUOF_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR0611377.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="ACHUOF_002208"
     CDS             complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="ACHUOF_002208"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR0611378.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="ACHUOF_002209"
                     /pseudo
     CDS             complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="ACHUOF_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8840..9832
                     /gene="xylF"
                     /locus_tag="ACHUOF_002210"
     CDS             8840..9832
                     /gene="xylF"
                     /locus_tag="ACHUOF_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0611379.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9910..11451
                     /gene="xylG"
                     /locus_tag="ACHUOF_002211"
     CDS             9910..11451
                     /gene="xylG"
                     /locus_tag="ACHUOF_002211"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFR0611380.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11429..12610
                     /gene="xylH"
                     /locus_tag="ACHUOF_002212"
     CDS             11429..12610
                     /gene="xylH"
                     /locus_tag="ACHUOF_002212"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFR0611381.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12688..13866
                     /gene="xylR"
                     /locus_tag="ACHUOF_002213"
                     /pseudo
     CDS             12688..13866
                     /gene="xylR"
                     /locus_tag="ACHUOF_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
     gene            complement(13974..14798)
                     /locus_tag="ACHUOF_002214"
     CDS             complement(13974..14798)
                     /locus_tag="ACHUOF_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFR0611382.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15118..17148
                     /gene="malS"
                     /locus_tag="ACHUOF_002215"
     CDS             15118..17148
                     /gene="malS"
                     /locus_tag="ACHUOF_002215"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR0611383.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17326..18579
                     /gene="avtA"
                     /locus_tag="ACHUOF_002216"
     CDS             17326..18579
                     /gene="avtA"
                     /locus_tag="ACHUOF_002216"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFR0611384.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18730..19203)
                     /locus_tag="ACHUOF_002217"
     CDS             complement(18730..19203)
                     /locus_tag="ACHUOF_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0611385.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19305..20153)
                     /gene="yiaJ"
                     /locus_tag="ACHUOF_002218"
     CDS             complement(19305..20153)
                     /gene="yiaJ"
                     /locus_tag="ACHUOF_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFR0611386.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
BASE COUNT         5075 a         5151 c         4951 g         5021 t
ORIGIN      
        1 gcctgccaga tggcaggctt tttaataacg gcgttattac tgcaacagcg aaatatccgc
       61 aacgcgcagg aacagttcgc gcagtttctc cagcatggtc agacggttga gacgcaattc
      121 tttgtcatca accatgacca tcactttatc gaagaaagca tcaaccggct cacgcagctc
      181 tgccagttcg accagcgcat cctggtaacg accttccgca aagtacggct ccagcttgtc
      241 acgtagcacc acaacctgca tcgccagttt aatttcttcc ggctctttca gggtagaggc
      301 attcacgcga tcgctcagca cttcgtcaga tttcgccagg atgttagata cacgtttgtt
      361 cgccgccgcc agtgctgcag ctgcttccag ggtacggaag tgcgataccg ctttcatgcg
      421 ggcatcgaaa tcagccggac gagtcggacg acgcgccagt accgcctgga tggtgtcaac
      481 ggtataacct tcgtcctgat accaggcgcg gaagcgaccg agcataaagt cgataacatc
      541 atcaactacg ttggcattag tcagcttatc gccatacaga cgcaccgctt cttcggtcag
      601 cgtttgcaga tcaaggttga ggttcttctc aacgataatt cgcagcacgc caagtgcggc
      661 acgacgcagc gcaaacgggt ctttgtcgcc tttcggatgc tgaccgatac cgaagatacc
      721 cgccagagta tccatcttat cagcaatcgc cagcgcacaa gctaccgggt tggacggcag
      781 gtcatcaccg gcaaagcgcg gctgatactg ctcattcagc gcgacggcta cgtcttccgc
      841 ttcgccatcg tgacgcgcgt agtgcatccc cataacgcct tgggtgtcgg tgaactcgaa
      901 gaccatgttg gtcatcaggt cacacttaga cagcaggccc gcacgggttg cgtggttaac
      961 gtcagcgcca atctgttcgg cgatccagcc agccagcgcc tggatgcggt cagtcttgtc
     1021 acgcagcgta cccagctgtt gctggaacag cacggtttgc aggcgcggca ggttatcttc
     1081 cagacgtttt ttgcggtcgg tgttgaagaa gaactcggca tccgccagac gtggacgaac
     1141 gactttctcg ttaccagaga taatttgctg cggatctttc gattcgatgt tggcaacgaa
     1201 gataaagttc ggcagcagtt tgccgtcgtt cgcatacacc gggaagtact tctggtcacc
     1261 tttcatggtg taaaccaggg cttcagccgg caccgcgagg aatttctctt cgaattttgc
     1321 ggtcagaacg accggccact ccaccagcga agccacttct tccagcaggc tttcgcttaa
     1381 gtcagcgtta ccgccaatct tacgcgctgc ttcttcggca tcggctttaa tcttcgcttt
     1441 acgttcttcg taatcggcga tgactttccc gcgctcacgc agaatttccg gatactgatc
     1501 ggcgttgtcg atggtgaatt ccggttcgcc cataaagcgg tggccgcgaa tcacgcgatc
     1561 ggactgaatg cccagaatgg ttgccggaat gactttgtcg cccagcagca gggtcacggt
     1621 gtgcaccgga cgcacgaagt gcacgtcgct tgcaccccag cgcatcagtt tcgggatcgg
     1681 cagtttcgcc agcgaagtcg caaccatatt cggcagcagt gcttcggtgc tttcgccctt
     1741 cacatgggcg cgatacagca gccattcgcc tttatcggta gtcagacgct cagcctggtc
     1801 aacggtaata ccacaaccac gcgcccaacc ttctgccgct ttgctcggtt ttccttcagc
     1861 gtcgaacgcc tgggcaatcg ccgggccgcg tttttcgatt tcacgatctg gttgtgcttc
     1921 cgccaggtta gctactttca gcgccagacg acgcggagca gcaaaccatt gaacggtgcc
     1981 gtgtgcgagg cctgcgttat ccagctccgc agtaaagttc gcagcaaagg actctgccag
     2041 gctgcgcagt gcttttggtg gcagctcttc agtgccgatt tccaccagaa aagttttctc
     2101 agacatagcc gcctcttact tatctttgtt gcacatcggg aagccgaggg cttcacggga
     2161 agcgtaatat gcttctgcca ctgctttggt cagggtgcga atgcgcagaa tgtagcgctg
     2221 acgctcggtg acggagatgg ctttacgcgc atccagcagg ttgaagctgt gggcggcttt
     2281 cagaatacgc tcgtaggctg gcagcggcag cggattttcc agcgccagca gctgctgcgc
     2341 ttctttctcg tactgctcaa agcaggtgaa caggaagtcc acatccgcgt attcgaagtt
     2401 gtaagtggac tgctccactt cgttctgatg gaacacgtcg ccgtaggtgg ttttacccag
     2461 cgggccgtcg ctccagacta ggtcgtaaac gctgtctacg ccctgaatgt acatggccag
     2521 acgttccaga ccgtaggtga tctcgccggt aaccggttta cactccagac caccaacctg
     2581 ctggaagtaa gtgaactgcg tcacttccat gccgtttagc cacacttccc agcccagtcc
     2641 ccaggccccc agagtcgggt tttcccagtt atcttccacg aaacggatgt cgtgaatagt
     2701 cgggtccatg cccagctctt tcagagaacc gaggtacagc tcctgaatat tgtccggcga
     2761 tggcttaatg accacctgga actgatagta gtgctgtaaa cggttggggt tttcgccgta
     2821 gcgaccatcg gtcgggcgac gagaaggctg aacataagca gccgccatcg gttctggccc
     2881 cagcgcgcgc agacaggtca ttgggtgaga ggttcccgcg ccgacttcca tgtccaatgg
     2941 ttgaacaatg gtgcagccct ggcgagccca gtaatcctgt aaggtcagga tcaagccctg
     3001 gaaggtcctg gtatcaaact tttgcatatt atttcgtgct ggatacgtgt ggatttaaag
     3061 gaagggatca gtatacccgc tggatggaag atatacagta cgaaacggga aaaagcaggg
     3121 cttaacgcat ggaaagatgc aaaaaatggc catctgcgtc aaaagaacag acaaactgct
     3181 ctttacggaa ggtataaccg cgcatttcat agcttccctg gaactgttcg aaggcggtga
     3241 cgtcgatttt ttgctcgccg gtgttataac gttttgccgc ctgatccttg cataacgctt
     3301 ccatattcaa cgaacgcaga ggatcaaccc ttacccgttg tgctttctgc acgggtgacg
     3361 gtgtagaaca acccactagc accataagcg ccattgccgg aaagaaagcg ttcatcatca
     3421 ttaaattacc gtctggtttg caggtgagtc ttattattta tatcggtata gtggctgata
     3481 tcttacgaat ttccttaagc ctctataggc tgtagtgata aaactcagat tattccagtg
     3541 cgccaaccaa atgatttatc ggtcagactt tttttaacca gatacagaga cacagatgca
     3601 gcccaaaatt tactggattg ataacctgcg agggatagcg tgtttaatgg tggtgatgat
     3661 tcacaccact acctggtatg tgaccaatgc tcatagtgtt agccccgtca catgggatat
     3721 cgccaatgtt ctgaattctg cctctcgtgt cagcgtgccg ctatttttca tgatttccgg
     3781 ctatctcttt tttggcgaac gcagcgccca gccgcgccat ttcttgcgta tcggcttatg
     3841 tctgattttt tatagcgcaa tcgcactgct ctacattgcg ctgtttacct ccatcaatat
     3901 ggagttagcg ctgaaaaacc tgctgcaaaa gccagtgttt taccacttgt ggtttttctt
     3961 cgcgattgcg gtgatttatc tggtttcacc gctgattcag gtgaagaacg tcggcggaaa
     4021 aatgttgctg gtgctaatgg tggtgattgg cattatcgct aacccaaaca cagtgccgca
     4081 gaaaattgac ggttttgaat ggctgccaat taacttatat atcaatggcg atacttttta
     4141 ctacattctg tatggcatgt tgggccgcgc tatagggatg atggacacac agcataaagc
     4201 actgtcgtgg gtgagcgccg cgctgtttgc gacgggggtt tttattatct ctcgcgggac
     4261 attatatgaa ttgcagtggc gcggaaattt tgccgatacc tggtatcttt actgtgggcc
     4321 gatggttttt atctgcgcaa tcgcgctatt gactctggtt aaaaacacgc tggatacgcg
     4381 taccattcgc ggacttggct taatctcccg ccattcattg ggtatatacg gattccacgc
     4441 cttgattatc catgcgctgc gcacccgggg aattgagctt aaaaattggc caatactgga
     4501 tattatttgg atcttttgcg cgacgttggc agcgagtttg ttactttcta tgctggtaca
     4561 acgaatcgac agaaacagat tagtgagtta agtaaaagcc cgttcacatt ggactgaccg
     4621 ggcttacgtg aattattcgc tgtattcttc ctgggtaacc tgtgtttctt ttggcgggtt
     4681 tattccggca tcacgaatat tcttctgcac cgctactgca ccaaacaagc ttaagaagaa
     4741 agccagtcca taaaaacctt tttcactgag taataatgtc gcattccaca gacctaccat
     4801 cagtagagca acagagataa taaagacagt caggcaggtc atataataaa tggaagtggt
     4861 cggtatgcct tcatacttgt cccgaacggt cttttgataa gacgcagcag aaaacagtcc
     4921 taacaccagt acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag
     4981 ccctaacagg taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc
     5041 cggtgaatag gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta
     5101 agagctttat gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa
     5161 aaaggcgatg gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat
     5221 aaacaagaga ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc
     5281 cagttgcccg agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa
     5341 cacgccaaga aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag
     5401 ataaaccagc gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac
     5461 gatctccata tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat
     5521 gccattaaca aatgattttc agaataaatt catactaaat attaattaat tagtgagata
     5581 tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg
     5641 cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg
     5701 ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga
     5761 tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc
     5821 cccccccgta cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta
     5881 ctcactacgc gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg
     5941 cacgacatcc atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag
     6001 ttcattgggg ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt
     6061 gtgtggtgta cgctcgccgg aaagataagg cagaaaccaa actggctcgg cactttcatc
     6121 agcctgttga gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc
     6181 ccaatccaga cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag
     6241 cgcatggcaa aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc
     6301 aaaatagacc cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac
     6361 accaactgca ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc
     6421 ccacgctttc gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc
     6481 gggcatctgg tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt
     6541 tgcgacatcc agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt
     6601 catacgcaga cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc
     6661 cggctcatgc cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt
     6721 gccggtaatc actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca
     6781 gcgcccgtcg ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt
     6841 tgctccatgc atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc
     6901 gcccagagct ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga
     6961 ccaaagtgga tgcgggcgcg aaacggtcag cttttccgtt tgcgaagcaa ccacctcacc
     7021 ctgctcgttg agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat
     7081 atcgatcgtt ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc
     7141 acagttagcc gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg
     7201 cgaccactct gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc
     7261 agtgacattt ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacac
     7321 tgcgcgatgc gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc
     7381 agtgccatcg tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt
     7441 tgacgacgta ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga
     7501 atttcataca tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag
     7561 cccagttgcg catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg
     7621 gtggctattt catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc
     7681 agtttaatct ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg
     7741 taatcatatt gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctag
     7801 aagccgattt tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc
     7861 tcctgacgca agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca
     7921 tagttttcac cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc
     7981 cagctgaaga cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta
     8041 aagcagttag cggttcccca cagcagcttc acgctgctct cttcttgctt ggctgccagg
     8101 acatcaacca tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa
     8161 acatccacat cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg
     8221 acatctgctt tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta
     8281 aacgccccca caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg
     8341 gcaaaacgca agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga
     8401 cggaatgcta acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca
     8461 aaataggctt gcatattgaa ctccataatc aggtaatgcc gcgggtgatg gatgatgtcg
     8521 taatattggg cactcccttt caattgctca attatgttat ttcacactgc tattgagata
     8581 attcacaagt gtgcgctcgc tcgcaaaata aaatgggatg atgaaaatgg gtaattcctc
     8641 gaagataaaa atgcaataag tacaattgcg caacaaaagt aagatctcgg tcataaatca
     8701 agaaataaac caaaaatcgt aatcgaaaga taaaaatctg taattgtttt cccctgttta
     8761 gttgctaaaa attggttacg tttatcgcgg tgattgttac ttattaaagc tgtcctctaa
     8821 ctacagaagg ccctacacca tgaaaataaa gaacattcta ctcacccttt gcacctcact
     8881 tctgcttacc aacgttgcgg cacacgccaa agaagtcaaa ataggtatgg cgattgatga
     8941 tctccgtctt gaacgctggc aaaaagatcg agatattttt gtgaaaaagg cagaatctct
     9001 cggtgcgaaa gtatttgtac agtctgcaaa tggcaatgaa gaaacacaaa tgtcgcagat
     9061 tgaaaacatg attaaccggg gtgtcgatgt tcttgtcatt attccgtata acggtcaggt
     9121 attaagtaac gttgtaaaaa aagccaaaca agaaggtatt aaagtattag cttacgaccg
     9181 tatgattaac gatgcggata tcgattttta tatttctttc gataacgaaa aagtcggtga
     9241 actgcaggca aaagccttgg tcgatattgt tccgcaaggt aattacttcc tgatgggcgg
     9301 ctcgccggta gataacaacg ccaagctgtt ccgcgccgga caaatgaaag tgttaaaacc
     9361 ttacgttgat tctggaaaaa ttaaagtcgt tggtgaccaa tgggttgatg gctggttacc
     9421 ggaaaacgca ttgaaaatta tggaaaacgc gctaaccgcc aataataaca aaattgatgc
     9481 tgtagttgcc tcaaacgatg ccaccgcagg tggggcaatt caggcattaa gcgcgcaagg
     9541 tttatcaggg aaagtagcaa tttccggcca ggatgcggat ctcgcaggta ttaaacgtat
     9601 tgctgccggt acgcaaacta tgacggtgta taaacctatt acattgctgg caaatactgc
     9661 cgcagaaatt gccgttgaat tgggcaatgg tcaggagcca aaagcagata cctcactgaa
     9721 taatggcctg aaagatgtcc cctcccgcct cctgacaccg atcgatgtga ataaaaacaa
     9781 catcaaagat acggtaatta aagacggatt ccacaaagag agcgagctgt aagcgttacg
     9841 ccccggcgcg gagcgggggc gtgatttctc tccatgccgc gtgaatgaat tggcttaggt
     9901 ggagtcgtta tgccttatct acttgaaatg aagaacatta ccaaaacctt cggcagtgtg
     9961 aaggcgattg ataacgtcag cttgcggttg aatgctggcg aaatcgtctc actctgtggg
    10021 gaaaatgggt ctggtaaatc aacgctgatg aaagtgctgt gtggtattta tccccatggc
    10081 tcctacgaag gcgaaattat ttttgcggga gaagagattc aggcgagtca catccgcgat
    10141 accgaacgca aaggtatcgc catcattcac caggaattgg ccctggtgaa agaattgacc
    10201 gtgctggaaa atatcttcct gggtaacgaa ataacccaca atggcattat ggattatgac
    10261 ctgatgacgc tacgctatca gaagctgctc gcacaggtca gtttatccat ttcacctgat
    10321 acccgcgttg gcgatttagg gcttgggcaa caacaactgg ttgaaattgc caaggcactt
    10381 aataaacagg tgcgcttgtt aattctcgat gaaccgacag cctcattaac tgagcaggaa
    10441 acgtcggttt tactggatat tattcgcgat ctacaacagc acggtatcgc ctgtatttat
    10501 atttcgcaca aactcaacga agtcaaagcg atttccgata cgatttgcgt tattcgcgac
    10561 ggacagcaca ttggtacgcg tgatgctgcc ggaatgagtg aagacgatat tatcaccatg
    10621 atggtcgagc gagagttaac cgcgatttac cctaatgaac cacataccac cggagatgaa
    10681 atattacgta ttgaacatct gacggcatgg catccggtca atcgtcatat taaacgagtt
    10741 aatgatgtct cgttttccct gaaacgtggc gaaatactgg gtattgccgg actcgttggt
    10801 gccggacgta ccgagaccat tcagtgcctg tttggcgtgt ggcccggaca atgggaagga
    10861 aaaatttata ttgatggcaa acaggtagat attcgtaact gtcagcaagc catcacccag
    10921 gggattgcga tggtacccga agacagaaag cgcgacggca tcgttccggt aatggcggtt
    10981 ggtaaaaata ttaccctcgc cgcactcaat aaatttaccg gtggcattag ccagcttgat
    11041 gacgcggcag agcaaaaatg tattctggaa tcaattcagc aactcaaagt taaaacgtcg
    11101 tcccccgacc ttgctattgg acgtttgagc ggcggcaatc agcaaaaagc gatcctcgcc
    11161 cgctgtctgt tacttaaccc gcgcatcctc attcttgatg aacctaccag gggtattgat
    11221 attggcgcga aatatgagat ctacaaatta attaaccaac tcgtccagca gggtattgcc
    11281 gttattgtca tctcttccga attacctgaa gtgctcggcc ttagcgatcg tgtactggtg
    11341 atgcatgaag ggaaactaaa agccaacctg ataaatcata acctgactca ggagcaggtg
    11401 atggaagccg cattgaggag cgaacatcat gtcgaaaagc aatccgtctg aagtgaaatt
    11461 ggccgtaccg acatccggtg gcttttctgg gctgaaatca ctgaatttgc aggtcttcgt
    11521 gatgattgca gctatcatcg caatcatgct gttctttacc tggaccaccg atggtgccta
    11581 cttaagcgcc cgtaacgtct ccaacctgtt acgccagacc gcgattaccg gcatcctcgc
    11641 ggtaggaatg gtgttcgtca taatttctgc tgaaatcgac ctttccgtcg gctcaatgat
    11701 ggggctatta ggtggcgtcg cggcgatttg tgacgtctgg ttaggctggc ctttgccact
    11761 taccatcatt gtgacgttgg ttctgggact gcttctcggt gcctggaacg gatggtgggt
    11821 cgcgtaccgc aaagtccctt catttattgt caccctcgcg ggcatgttgg catttcgcgg
    11881 catactcatt ggcatcacca acggcacgac tgtatccccc accagcgccg cgatgtcaca
    11941 aattgggcaa agctatctcc ccgccagtac cggcttcatc attggcgcgc ttggcttaat
    12001 ggcttttgtt ggttggcaat ggcgcggaag aatgcgccgt caggctttgg gtttacagtc
    12061 tccggcctct accgcagtag tcggtcgcca agctttaacc gctatcatcg tattaggcgc
    12121 aatctggctg ttgaatgatt accgtggcgt tcccactcct gttctgctgc tgacgttgct
    12181 gttactcggc ggaatgttta tggcaacgcg gacggcattt ggacgacgca tttatgccat
    12241 cggcggcaat ctggaagcag cacgtctctc cgggattaac gttgaacgca ccaaacttgc
    12301 cgtgttcgcg attaacggat taatggtagc catcgccgga ttaatcctta gttctcgact
    12361 tggcgctggt tcaccttctg cgggaaatat cgccgaactg gacgcaattg cagcatgcgt
    12421 gattggcggc accagcctgg ctggcggtgt gggaagcgtt gccggagcag taatgggggc
    12481 atttatcatg gcttcactgg ataacggcat gagtatgatg gatgtaccga ccttctggca
    12541 gtatatcgtt aaaggtgcga ttctgttgct ggcagtatgg atggactccg caaccaaacg
    12601 ccgttcttga ttttgataaa aattttctca aagccggtta cgtattaccg gttttgagtt
    12661 tttgcatgat tcagcaggaa aagaaccatg tttactaaac gtcaccgcat cacattactg
    12721 ttcaatgcca ataaagccta tgaccggcag gtagtagaag gcgtagggga atatttacag
    12781 gcatcacaat cagaatggga tattttcatt gaagaagatt tccgcgcccg cattgataaa
    12841 atcaaggact ggttaggaga tgtcgtcatt gccgacttcg acgacaaaca gatcgagcaa
    12901 gcgctggctg atgtcgacgt ccccattgtt ggggttggcg gctcgtatca ccttgcagaa
    12961 agttacccac ccgttcatta cattgccacc gataactatg cgctggttga aagcgcattt
    13021 ttgcatttaa aagagaaagg cgttaaccgc tttgcttttt atggtcttcc ggaatcaagc
    13081 ggcaaacgtt gggccactga acgcgaatat gcatttcgtc agcttgtcgc cgaagaaaag
    13141 tatcgcggag tggtttatca ggggttagaa accgcgccag agaactggca acacgcgcaa
    13201 aatcggctgg cagactgact acaaacgctg ccaccgcaaa ccgggattat tgccgttact
    13261 gacgcccgag cgcggcatat tctgcaagta tgtgaacatc tacatattcc cgtaccggaa
    13321 aaattatgcg tgattggcat cgataacgaa gaactgaccc gctatctgtc gcgtgtcgcc
    13381 ctttcttcgg tcgctcaggg cgcgcggcaa atgggctatc aggcggcaaa actgttgcat
    13441 cgattattag ataaagaaga aatgccgcta cagcgaattt tggtcccacc agttcgcgtc
    13501 attgaacggc gctcaacaga ttatcgctcg ctgaccgatc ccgccgttat tcaggccatg
    13561 cattacattc gtaatcacgc ctgtaaaggg attaaagtgg atcaggtact ggatgcggtc
    13621 gggatctcgc gctccaatct tgagaagcgt tttaaagaag aggtgggtga aaccatccat
    13681 gcaatgattc atgccgagaa gctggagaaa gcgcgcagtc tgctgatttc aaccaccttg
    13741 tcgatcaatg agatatcgca aatgtgcggt tatccatcgc tgcaatattt ctactctgtt
    13801 tttaaaaaag catatgacac aacgccaaaa gagtatcgcg atgtaaatag cgaggtcatg
    13861 ttgtagcgat gatgagaatt gtcggcgtca catcagggaa ctcagcacag ccacctgatg
    13921 cgagcaaaag ccttatccga ccgacaaatc agataaggct cgaggaaatg aaattacata
    13981 tgcgccgcga ttaaccgttg gttatcctgg tacattgcga acaggtagtt gttataactc
    14041 ttccctttgg tcgagtagcc cttcagcttg tgaatcatcg ctgtggcagt cacttcctga
    14101 tccgctttac gcagctgtgc acgcgattta cggaacgaag agtaagccgg gtgcgtattc
    14161 aggttagtga cataggcgct caccgattct ttgacagaac taaactgtga gtaccctttc
    14221 actttacccg gcgcattggt acaacgtcct ttcatgcatt tcatgccgaa cagattgtta
    14281 ttgttgcgcg ccagcttcga cgttccccaa ccgctttctg ctgcagccat cgtcgccacc
    14341 atactggtag ggataatgtc tacgcgttca agcaaggtat tccacgggat ttttcgcgta
    14401 ttaccagacc acttcacctt gtagcgtttg gcgatgtctt tcagacgcgc acgctcagca
    14461 ggtgcccatt ggccctgata ctgttttgaa atcagccagt tacgttctgc agtaatggct
    14521 gcattttggc tggtaatgta aggcattacg gtccggagaa acgccttttt ccttggtgtt
    14581 ccggaagggt attttcgcaa atcaggaagt gaactactct ttgcactatt gcgagaatac
    14641 tcttgtttac tgcttacctg tttattacta gctttagtta agtgggactt ttgactcgct
    14701 gttgttgtgt gcgtcttcgc taacacctca ctcgaaaaca ccagagtgag taacataaga
    14761 atcatcgccc catatcgtcg tatgggagtc aaaatcatca ggtctcctgg tcggatttaa
    14821 tcattccaac accttatatt tttcacaaat ttgagagttg aatctcaaat catatcaaaa
    14881 atagctgtca agagcacccc aaggaatagt ccaaatctga aactatgtca cgagttaacg
    14941 attcagattg gcgctaaatc gcagaaaatg tgggggttat cgcaaaattc agccgttttt
    15001 tgcgcgcgat cgctcaccct tgcttctcat cctgtggact taccgctcag ggatgagttt
    15061 tgtctggctt atcgctggca aactgtctga aatcgcagca ataaggactc atccaccatg
    15121 aaactcgccg cctgttttct gacactcctt cctggcttcg ccgttgccgc cagctggact
    15181 tctccggggt tccctgcctt tagcgaacag ggaacgggaa catttgtcag ccacgcgcag
    15241 ttgcccaaag gtacgcgtcc actcacgcta aattttgacc aacagtgctg gcagcctgca
    15301 gatgcgataa aactcaatca gatgctttcc ctgcaacctt gtagcaacac gccgcctcaa
    15361 tggcgattgt tcagggacgg caaatatacg ctgcaaatag acacccgctc cggtacgcca
    15421 acattgatga tttccatcca gaacgccgcc gaaccggtag caaacctggt ccgtgaatgc
    15481 ccgaaatggg atggattacc gctcacgctg gatgtcagcg ccactttccc ggaaggagcc
    15541 gccgtacggg attattacag ccaacaaatt gcgatagtga agaacggtca aataacgtta
    15601 caacccgctg ctaccagcaa cggtttactc ctgctggaac gggcagaaac tgacacatcc
    15661 gcccctttcg actggcataa cgccacggtt tactttgtgc tgacagatcg tttcgaaaac
    15721 ggcgatccca gtaatgacca gagttacgga cgtcataaag acggtatggc ggaaattggc
    15781 acttttcacg gcggcgattt acgcggcctg accaacaaac tggattacct ccagcagttg
    15841 ggcgttaatg ctttatggat aagcgcccca tttgagcaaa ttcacggctg ggtcggcggt
    15901 ggtacaaaag gcgatttccc gcattatgcc taccacggtt attacacaca ggactggacg
    15961 aatcttgatg ccaatatggg caacgaagcc gatctacgga cgctggttga tagcgcacat
    16021 cagcgcggta ttcgtattct ctttgatgtc gtgatgaacc acaccagcta tgccacgctg
    16081 gcggatatgc aggagtatca gtttggcgcg ttatatcttt ctggtgacga agtgaaaaaa
    16141 acgctgggtg aacgctggag cgactggaaa cctgccgccg gacaaacctg gcatagcttt
    16201 aacgattaca ttaatttcag cgacaaaaca ggctgggata aatggtgggg aaaaaactgg
    16261 atcagaacgg atatcggcga ttacgacaat cctggattcg atgatctcac tatgtcgctg
    16321 gcctttttgc cggatatcaa aaccgaatca actaccgctt ctggtctgcc ggtgttctat
    16381 aaaaacaaaa cggatactca cgctaaagtc atcgaaggct ttacacctcg cgattactta
    16441 acccactggt taagtcagtg ggtccgcgac tatgggattg atggttttcg ggtcgatacc
    16501 gccaaacatg ttgagttgcc cgcctggcag caactgaaaa ccgaagccag ctccgcgctt
    16561 cgcgaatgga aaaaaactaa ccccgacaaa gcattagatg acaaaccttt ctggatgacc
    16621 ggtgaagcct ggggccacgg cgtgatgcaa agtgactact atcgccacgg cttcgatgcg
    16681 atgatcaatt tcgattatca ggagcaggcg gcgaaagcag tcgattgtct ggcgcagatg
    16741 gatacgacct ggcagcaaat ggcggagaaa ttgcagggtt tcaacgtgtt gagctacctc
    16801 tcgtcgcatg atacccgtct gttccgtgaa gggggcgaca aagcagcaga gttattacta
    16861 ttagcgccag gcgcggtaca aatcttttat ggcgatgaat cctcgcgtcc gttcggtcct
    16921 acaggttctg atccgctgca aggtacacgt tcggatatga actggcagga tgttagcggt
    16981 aaatctgccg ccaacgtcgc gcactggcag aaaatcagcc agttccgcgc ccgccatccc
    17041 gcaattggcg cgggcaaaca aacgacactt tcgctgaagc agggctacgg ctttgttcgt
    17101 gagcatggcg acgataaagt gctggtcatc tgggctgggc aacagtgact tttccggctt
    17161 cccgttcgtc agtacctcgg gaagccgcca accaggataa aatgtcagcc ctaatcagcg
    17221 ttgcgggata aagcaccgct cactcttcaa cagaccgatt tgcaccccag caaatgtagc
    17281 gttattgtta ccttccttgc tacagagttc gacagatatc ccgctatgac attctccctt
    17341 tttggtgaca aatttacccg ccactccggc attacgctgc tgatggaaga tctgaacgac
    17401 ggtttacgca cgcctggcgc gattatgctc ggcggcggta atccggcgca gatcccggaa
    17461 atgcaggact acttccagac gctactgacc gacatgctgg aaagtggcaa agcgactgat
    17521 gcactgtgta actacgacgg tccacagggg aaaacggagc tactcacact gcttgccgga
    17581 atgctgcgcg agaagttggg ttgggatatc gaaccacaga atattgcact aacaaacggc
    17641 agccagagcg cgtttttcta cttatttaac ctgtttgccg gacgccgtgc cgatggtcgg
    17701 gtcaaaaaag tgctgttccc gctggcaccg gaatacattg gctacgctga cgccggactg
    17761 gaagaagatc tgtttgtctc tgcgcgtccg aatattgaac tgctgccgga aggccagttt
    17821 aaataccacg tcgattttga gcatctgcat attggcgaag aaaccgggat gatttgcgtc
    17881 tcccggccga cgaatccaac aggtaatgtg attactgacg aagagttgct gaagcttgac
    17941 gcgctggcga atcaacacgg cattccgctg gtgattgata acgcttatgg cgtcccgttc
    18001 ccgggtatca tcttcagtga agggcgcccg ctatggaatc cgaatatcgt actgtgcatg
    18061 agtctttcca agctggggct gcctggctcc cgctgcggca ttatcatcgc caatgaaaaa
    18121 atcatcactg ccatcaccaa tatgaacggc attatcagcc tggcacctgg cggtattggt
    18181 ccggcgatga tgtgtgaaat gattaagcgt aacgatctgc tgcgcctgtc tgaaacagtc
    18241 atcaaaccgt tttactacca gcgtgttcag gaaactatcg ccatcattcg ccgctattta
    18301 ccggaaaatc gctgcctgat tcataaaccg gaaggagcca ttttcctctg gctatggttt
    18361 aaggatttgc ccattacgac cgagcagctc tatcagcgcc tgaaagcacg cggcgtgctg
    18421 atggtgccgg ggcacaactt cttcccggga ctggataaac cgtggccgca tacgcatcaa
    18481 tgtatgcgca tgaactacgt accagagccg gagaaaattg aggcgggggt gaagattctg
    18541 gcggaagaga tagaaagagc ctgggctgaa agtcactaaa aattgcctga tgcgctacgc
    18601 ttatcaggcc tacatgattt ctgcaatata ttgaatttgg aagaatttgt aggccggata
    18661 aggcgtttac gccgcatctg gcataaacaa agcgcactat tccggcattg agaaacgccg
    18721 gaaaacgttt caaacaggct gctgctgtag ccgatgccgc tgcactttct cgacatccat
    18781 gcactgcaac gcctttgtcg ggcaggcttc aacacaggcc ggccccgtct cccgatgcca
    18841 gcacagatcg cacttaatcg ccctcgcctt tttgcgcgaa gaaacgacct ccatcgcacc
    18901 aaacgggcaa gccagcatac agcttttaca gccaatgcaa cgtgtttgtt caacgaaaat
    18961 atgcccatgt tcgcggctta tcgcgtcaac agggcagaca ttcgcgcacg gtgcatcttc
    19021 acactgatga caggctactg gcgtggtcca gctgtggtct ttaatgacac gaatacggga
    19081 aataaactcg tctggtgaca acgcagcgca atcctgattc tcctgatgcg acactgcgca
    19141 agccacttca caggtacggc aaccgataca tttcgtcgca tccgcaataa taaaccggtt
    19201 catgatcatt cctttgcact aaaacagcag agtgccagag agcgcgtccc tactgctttg
    19261 atccaacgaa ggaaatgtta aagttttggc acttgtccaa agcgttatgt gatcgcgccc
    19321 aggtcatccc tgacagtaaa tcccagttca ttagaaatag cctgcgcggt ttcacgcagt
    19381 ggtttcagga gatttttctc tcccacctgt ttcagacgtg atgtcgaaag cgaaatcgac
    19441 acggcgtacg gcacccgccc atgaatatca aacaccggaa cagcaataca ggagacgccg
    19501 agttcgtttt cttctctgtc catcgccgct ccgctttcac gaatgtgcgc cagttcgtcg
    19561 aacatcgcgg gcagctcggt aatggtattg cgggttaacg gctggatctc atgctgatgg
    19621 ttttcccagt atgacttcac gtagtccggg tgaccaaacg ccatgtagat cttgcccatt
    19681 gcggaacagt agagcggcat atgctggcca atataggcac gggttcgcag catcccggtt
    19741 gtgggttcca gcttataaat caaaatagcg tgatcgtctt cgcggctgga gaagttaatg
    19801 gtttcaccag tggcgatgtt cagtgcctca agatgcggag cggcgatatg aatgatattc
    19861 agcgaagaca gcgccttctg cccgacggca ataaatttgg tagtcaggcg gtaactccct
    19921 gcggcgggcg cggtggtcac atagccgcag gactgtaatc cctgcaataa gcgatggacg
    19981 gtactcttat ttaaaccagc cagctccgaa agatgcgcca acggacaacc gtttggatag
    20041 ttgctcaaaa tctcaatcag catcaacccg cgaaacagac tctggcttcc ggctggacgc
    20101 tctttttcct gcgccatctc gttctctttt tttcccatca cttctttccc cattttgtcg
    20161 cgtcctgatg gtagcgcaaa gtgtgccgta gttcacga
//
LOCUS       DAZOYF010000066        20133 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZOYF010000066 DAZOYF010000000
VERSION     DAZOYF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.066"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            88..864
                     /gene="exuR"
                     /locus_tag="ACHUOF_002219"
     CDS             88..864
                     /gene="exuR"
                     /locus_tag="ACHUOF_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR0611387.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1104..1187
                     /gene="yqiM"
                     /locus_tag="ACHUOF_002220"
     CDS             1104..1187
                     /gene="yqiM"
                     /locus_tag="ACHUOF_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR0611388.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1209..1871
                     /gene="yqjA"
                     /locus_tag="ACHUOF_002221"
     CDS             1209..1871
                     /gene="yqjA"
                     /locus_tag="ACHUOF_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR0611389.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1875..2258
                     /gene="mzrA"
                     /locus_tag="ACHUOF_002222"
     CDS             1875..2258
                     /gene="mzrA"
                     /locus_tag="ACHUOF_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR0611390.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2405..2759
                     /gene="yqjC"
                     /locus_tag="ACHUOF_002223"
                     /pseudo
     CDS             2405..2759
                     /gene="yqjC"
                     /locus_tag="ACHUOF_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
     gene            2797..3102
                     /locus_tag="ACHUOF_002224"
     CDS             2797..3102
                     /locus_tag="ACHUOF_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR0611391.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3105..3509
                     /locus_tag="ACHUOF_002225"
     CDS             3105..3509
                     /locus_tag="ACHUOF_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR0611392.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3499..3798
                     /locus_tag="ACHUOF_002226"
     CDS             3499..3798
                     /locus_tag="ACHUOF_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR0611393.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3984..4280
                     /locus_tag="ACHUOF_002227"
     CDS             3984..4280
                     /locus_tag="ACHUOF_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0611394.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4350..5336
                     /gene="yqjG"
                     /locus_tag="ACHUOF_002228"
     CDS             4350..5336
                     /gene="yqjG"
                     /locus_tag="ACHUOF_002228"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFR0611395.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5629..5997
                     /locus_tag="ACHUOF_002229"
     CDS             5629..5997
                     /locus_tag="ACHUOF_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0611396.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6204..7100)
                     /gene="yhaJ"
                     /locus_tag="ACHUOF_002230"
     CDS             complement(6204..7100)
                     /gene="yhaJ"
                     /locus_tag="ACHUOF_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFR0611397.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7205..7906
                     /locus_tag="ACHUOF_002231"
     CDS             7205..7906
                     /locus_tag="ACHUOF_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR0611398.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7929..8093
                     /gene="yhaL"
                     /locus_tag="ACHUOF_002232"
     CDS             7929..8093
                     /gene="yhaL"
                     /locus_tag="ACHUOF_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR0611399.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8305..9615)
                     /gene="cyuA"
                     /locus_tag="ACHUOF_002233"
     CDS             complement(8305..9615)
                     /gene="cyuA"
                     /locus_tag="ACHUOF_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR0611400.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9643..10974)
                     /locus_tag="ACHUOF_002234"
     CDS             complement(9643..10974)
                     /locus_tag="ACHUOF_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0611401.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11249..12613)
                     /gene="tdcG"
                     /locus_tag="ACHUOF_002235"
     CDS             complement(11249..12613)
                     /gene="tdcG"
                     /locus_tag="ACHUOF_002235"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0611402.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12685..13074)
                     /locus_tag="ACHUOF_002236"
     CDS             complement(12685..13074)
                     /locus_tag="ACHUOF_002236"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR0611403.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13088..15382)
                     /gene="tdcE"
                     /locus_tag="ACHUOF_002237"
     CDS             complement(13088..15382)
                     /gene="tdcE"
                     /locus_tag="ACHUOF_002237"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR0611404.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15416..16624)
                     /gene="tdcD"
                     /locus_tag="ACHUOF_002238"
     CDS             complement(15416..16624)
                     /gene="tdcD"
                     /locus_tag="ACHUOF_002238"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR0611405.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16650..17981)
                     /gene="tdcC"
                     /locus_tag="ACHUOF_002239"
     CDS             complement(16650..17981)
                     /gene="tdcC"
                     /locus_tag="ACHUOF_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR0611406.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18003..18992)
                     /gene="tdcB"
                     /locus_tag="ACHUOF_002240"
     CDS             complement(18003..18992)
                     /gene="tdcB"
                     /locus_tag="ACHUOF_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR0611407.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19091..20029)
                     /gene="tdcA"
                     /locus_tag="ACHUOF_002241"
     CDS             complement(19091..20029)
                     /gene="tdcA"
                     /locus_tag="ACHUOF_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR0611408.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         5208 a         5273 c         4814 g         4838 t
ORIGIN      
        1 aatcatcaaa tttatatatg accaatggca tacccatgaa taaagtggta tgataacttc
       61 gtccgcattc cactccggga gacgcatatg gaaatcactg aaccacgccg tttgtatcaa
      121 caacttgctg ctgacctgaa agagcgcatt gaacagggcg tctatctggt gggtgataaa
      181 ctgcctgccg aacgctttat tgccgatgaa aagaacgtca gccgaacggt agttcgtgaa
      241 gccatcatca tgctggaagt tgaaggctat gtggaggtgc gtaaaggttc aggtattcat
      301 gtggtttcca accagccgcg tcatcagcag gcggctgaca ataatatgga attcgccaat
      361 tacggtccgt ttgagttgct tcaggctcgc cagctcatcg aaagtaatat tgccgagttc
      421 gcggcaactc aggtaacgaa acaagacatc atgaaactga tggccattca ggaacaggcg
      481 cgcggcgaac aatgcttccg tgattccgag tgggatttac agttccacat tcaggtagcc
      541 ctggcgacgc agaactccgc cctggcggcc atcgttgaaa aaatgtggac ccagcgtagc
      601 cataacccgt actggaaaaa actacacgaa cacattgatt cccgtaccgt tgataactgg
      661 tgtgatgacc acgatcaaat tctcaaggcg ttaattcgca aagatcctca tgctgctaag
      721 ctggcaatgt ggcagcatct ggaaaacacc aaaatcatgt tatttaacga aaccagcgac
      781 gatttcgagt tcaatgccga ccgctatctg ttcgccgaaa acccggtagt gcatctcgat
      841 acggccacca gcggcagcaa atgaaattcc tgtccgacag gcgctgcaat gcgcctgttt
      901 gtctcgtcgg taagcgaaaa gtataaagtg tcagcctgtg taaatcctct cgccaccctc
      961 ccctgcaatc agcaaaatca gactccacgg acatgtaatt ttgataacga acaacgttga
     1021 cctttgttac aattagattc aatttgaatt tatgcttttg aatgctttct tatctcacgt
     1081 tttaacaggg aatagttcag gctgtgttga tgtatcaaac ccgcagaaca taccaaaaca
     1141 gcaataacat tgcggtagtg catcttttaa aaccagcgtg gcgttaaccg attcaccagg
     1201 aataatgaat ggaacttttg acccaattgc tgcaagccct gtgggcgcag gattttgaaa
     1261 ccctggccaa tccatcgatg attggcatgt tgtattttgt cttgtttgta attttgttcc
     1321 ttgaaaatgg cttgcttccg gcggcctttt taccgggcga cagtttactg gtattggtcg
     1381 gcgtgttgat tgcgaaaggc gcgatgggtt acccgcaaac gattctgctg ctgaccgttg
     1441 ccgccagcct cggttgctgg gtcagctata ttcaggggcg atggctgggc aatacccgca
     1501 ccgtacaaaa ctggctatct catttacccg cgcattatca tcaacgcgca caccatcttt
     1561 ttcataaaca cggtttatcg gcgctgttaa ttggtcgctt tattgcattt gtcagaacac
     1621 tgctgccgac gattgccggg ttatcagggc tgaataacgc gcgctttcag ttcttcaact
     1681 ggatgagcgg tctgctgtgg gtattgatcc tgacaactct gggttacatg ctcggcaaaa
     1741 cgccggtatt tttaaagtac gaggaccagc tgatgtcatg cctgatgctg ctcccggtgg
     1801 tgctgctggt gtttggcctg gcaggttctc tggtcgtgtt atggaaaaag aaatatggaa
     1861 atcgggggta agggatgcaa atacctcgca tgtcgcttcg ccagctagcc tggtccggca
     1921 ctgttttact tctggtcggc acgctgttgc tggcctggtc cgcggttcgc cagcaagagt
     1981 ctacgctggc gattcgtgcc gttcatcaag gcacaacgat gccagacggt ttttcgatct
     2041 ggcatcacct tgacgctcat ggcattcctt tcaaaagtat cacccccaaa aacgacactc
     2101 tgttaattac atttgactcc agcgaccaga gcgccgccgc aaaagcggtc ctcgacagaa
     2161 cattgcccca tggctacatc attgcgcagc aggacaataa cagtcaggct atgcagtggc
     2221 tgacccggtt acgggataac tctcatcgct tcggataact tcctgttatc cgaaacatat
     2281 cactcacttt ggtgatttca ccgtaactgt ctatgattaa tgaggcggtg gttaacacta
     2341 ccgcttcgtt gtctggacaa tcgttccttt gtaataggtc cgaatcacaa tggaaggttc
     2401 aagaatgaaa taccgcatcg ctttagctgt ttctctcttt gctcttagtg ccggtagtta
     2461 tgccactacc ctgtgtcagg aaaaggagca aaatatcctt aaggagatca gctatgccga
     2521 aaaacaccaa aaccagaatc gtattgacgg tctgaataaa gccctgagtg aagtccgggc
     2581 caactgttca gatagccagc tgcgtgccga tcatcacaag aaaatcgcaa agcagaaaga
     2641 tgaggtggcg gaacgccagc aagatttagc cgaggcgaag caaaaaggcg atgccgatag
     2701 aacggaaact ggcagaagcg caggaagagc tgaaaaagct ggaagcgcgc gactactaac
     2761 tcacaatagt cactacttac tcacctggag aaaactatgt cgaaagaaca cactacggaa
     2821 catctgcgtg ctgagttgaa atccctttcc gatacgctgg aagaggtgct tagctcatct
     2881 ggcgagaagt cgaaagaaga gttgagtaag attcgtagca aagcggagca ggcactgaaa
     2941 cagagccgtt atcgcctggg tgaaaccggt gatgccatcg ccaaacaaac ccgtgtcgcg
     3001 gcggcgcgtg ctgatgagta tgtgcgtgaa aatccgtgga ccggcgtggg aattggcgct
     3061 gcaatcggtg tagtgctcgg cgttctgctg tcgcgtcgtt aattatggcg gacactcatc
     3121 acgcgcaagg gcccggtaaa agcgttctgg gcatcgggca gcgaattgtt tccatcatgg
     3181 ttgaaatggt ggaaacacgt ctgcggctgg cggtggtgga gctggaagag gaaaaagcta
     3241 atctctttca acttttactg atgctgggcc tgacgatgct tttcgctgca tttggtctta
     3301 tgagcctgat ggtgctaatt atttgggcgg ttgatccgca atatcgcctg aatgcgatga
     3361 ttgccaccac cgtggtgttg ctgctactgg cactgattgg cggtatctgg acgttacgta
     3421 aatcgcgtaa atccaccttg ctgcgccata cacgccatga gctggcaaac gatcggcaac
     3481 tgctcgagga ggagtcccgt gagcagtaaa gtcgaacgtg aacgacgtaa ggcgcaactg
     3541 cttagccaga tccagcaaca acggctggat atttccgcca gtagtcgtga atggctggag
     3601 gcaacaggcg cttacgatcg tcgctggaat atgctgctaa gtctgcgctc ctgggcgctg
     3661 gttggcagta gagtgatggc gatctggacg attcgccatc ctaatatgct ggtccgctgg
     3721 gccagacgcg gttttggcgt atggagcgcc tggcgtctgg ttaaaacgac cctcaagcag
     3781 caacagcttc gcggttaact tccctctggc cggagcaatt ccggccttat ccatcaaatt
     3841 ttttgaatat ttttgacagt tttccttgct aacaatcatc attcaccacg tttatgattc
     3901 tctccatcga cagcaacgac gctaatagcg cgcccttgca caaaaaaaca atcagcagcc
     3961 tgagtggcgc agtggagagt atgatgaaaa aattagaaga tgttggtgta ctggtagcgc
     4021 acattttaat gccgattctg tttattaccg caggctgggg aaaaatcacc gcttactctg
     4081 gtacccaaca atatatggaa gcaatgggtg tcccgggttt tatgctgctg acggcatttt
     4141 tatttcacag caactttgct gaaggcgtca actcgctgat gttcatgaaa aacctgacaa
     4201 tttctggcgg attcctgctg ctggcaatta ccggtccggg cgcgtatagc atcggccgcc
     4261 tgctgaataa aaagtggtaa gcacgcgcta tacttaacga taaaaaacga ggaggaagct
     4321 cctcgttttt gctattggag gagagaaaaa tgggtcaact gattgacggc gtctggcatg
     4381 acacctggta cgataccaaa tctaccggcg gtaaatttca acgttcagct tccgcatttc
     4441 gtaactggct cactgccgat ggcgcacctg gccccactgg cacaggcggt tttatcgcag
     4501 agaaagatcg ttatcatctc tatgtttcac tcgcctgccc gtgggcgcac cgcacgctga
     4561 tcatgcgcaa actcaaagga ctggaaccgt ttatttccgt ttccgtagtg aacccgctga
     4621 tgctggaaaa cggctggacc tttgatgaca gttttccggg agcaaccgga gatacgctct
     4681 atcaacatga atttctgtat cagctttatc tccacgccga tccacactac agcggacgag
     4741 ttactgttcc cgtgctgtgg gacaaaaaga accacaccat cgtcagcaac gaatcagcag
     4801 aaatcatccg catgttcaat accgcgtttg atgcgctggg cgcaaaagcg ggtgattact
     4861 acccaccagc cctgcaaacg aaaattgacg aacttaacgg ctggatttat gacaccgtta
     4921 acaacggcgt gtataaagct ggttttgcca ccagccagca agcctacgac gaggcggtgg
     4981 cgaaagtgtt tgaatcgctg gcgcgactgg agcagatttt aggccagcat cgttacctga
     5041 ccggcaacca gctaaccgaa gccgatattc gcctgtggac tacgctggtg cgttttgatc
     5101 cagtgtatgt gacccacttc aagtgtgata agcaccgcat cagcgattac ctgaatctgt
     5161 atggcttcct gcgcgatatc taccagatgc cggggatcgc cgaaactgtc aatttcgatc
     5221 acatccgtaa tcattacttc cgcagccata agaccatcaa ccctacgggg attatttcaa
     5281 ttggtccgtg gcaggatctc gatgaaccgc atggacgaga tgttcgcttc ggttaaaaaa
     5341 aggcacctta cgaggtgcct ctgataatta gctaagtgca tgttgttcat gtcggatgcg
     5401 gcgtgaacgc cttatcaaac ctacaaagcg tttatattca atatgttaca atgaccgcgt
     5461 aggccagata agcgtagcgc atctggcgat tttgcgcttg ccatcagtct tagcgcgcct
     5521 tcacggcact ttattaattc cttgaacgaa tatttactgc catttcattc acgtctattc
     5581 ttaatttgct gctttaaaaa caagtgattg agcaaaattg aggcaaaaat ggactggtat
     5641 ctgaaagtac taaaaaatta tgtcggtttc cggggtcgtg cgcggcgcaa agagtactgg
     5701 atgtttattc tggtcaacat catctttacg ttcgtgctgg ggctgctgga taaaatgtta
     5761 ggctggcaac gcgctggtgg cgaaggcatc ctgacgataa tctacggtat tctggtgttt
     5821 ttaccgtggt gggcggttca gttccgccgc ctgcacgaca ccgaccgctc ggcgtggtgg
     5881 gcactgctat tcttaatccc gtttatcggc tggcttatta tcatcgtctt taactgccag
     5941 gcgggtacgc caggcgaaaa ccgctttgga ccagatccaa aactggaaca agaataatga
     6001 ttaagcacct tgtaaaaggt gcttcaaatt gttgacaaag tgcgctttgt ttatgccgga
     6061 tgcggcgtaa acgccttatc cagcctacaa attgccaaaa ttcaatatat tgcagtgact
     6121 gcgtaggcct gataagcgta gcgcatcagg cagttttgcg tttgtcagca gtcagaagca
     6181 ccttgtgaaa ggtgcttaat agattatttt ccagcgaaca actttggaat ttcccgcaga
     6241 caccaggatt tcgcttcccc catactgtca cgacgccagg ccataataat atcgatctcg
     6301 ctggtcgatt ccgggctgac gacacgcaac cgcccttccg caatatcttt ttcgaccatc
     6361 ggatacggca tcgtcgccac gccaagcccc gccagtaatg cctgacgttt atcttcaatc
     6421 gtgctcaccg ttaagcgcgg ctgtttgtcc agcagctgta cggtcaacac cgggcgctca
     6481 cgagcggtat ccgctaccgc aattccacga tatttcacac gcgtcacttc agataacggt
     6541 tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt agacgttcat taacgtatag
     6601 agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca tatccggcgc gataacgata
     6661 tccgcccgcc cctgctccag ccgttcccac gcccccgcca gcacttctgt gatgattgcc
     6721 agttgggtat tggcttttgc cgccagtttg tcgattaacg ggaaaaaggc aggtgtcggt
     6781 accagcgctt cggtcacaat ggtgagatgc gtttcccaac cgcgcgcgag agcttccgca
     6841 tcagtagtca gtttatctgc ggcttccagc aaaacgcgcc ctcgctccag caacatcctc
     6901 ccgacattgg tgaatttggt gcgatggccc gagcggtcaa acagcaccac atccagctct
     6961 tcttccagtt tttgcatggt gtagctaagt gcggaaggca cgcgtcccag ctcatccgct
     7021 gccgccgcaa aactgccccg gcgatcgatc gcatccataa cccgtagtgc ttccagcgtt
     7081 aatgcccttt ctttggccat ttcgttctca ttcaggaaat ttgaacatac cgggcagaat
     7141 atctggctaa caatgtagcg tccagcccct taccataaaa ggaagtaaag agaggtcaag
     7201 aattatgatt actacccgaa ctgccaggca gtgtggacaa gcagactacg gatggttgca
     7261 ggcccggtat actttttcct ttggacacta cttcgacccg aaattgttag gctatgcctc
     7321 cctgcgtgtg cttaaccagg aagtgctggc cccaggtgcc gcctttcagc cgcgaaccta
     7381 tcccaaagtc gatattttaa atgtgattct ggatggagaa gcagagtatc gcgacagcga
     7441 aggcaatcat gttcaggcca gcgccggaga ggcgttgctg ctctctaccc agccgggtgt
     7501 cagctatagc gaacacaatc tcagcaaaga caaaccgtta acgcgaatgc agctttggct
     7561 ggatacctgc ccgcagcgag agaatccgct gattcaaaag ctggcgctta atatggacaa
     7621 gcagcaatta atcgcctcgc cagagggtgc gatgggaagc ctgcaattac gccaacaagt
     7681 gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg aatttccagt tgcatgggcc
     7741 acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg cttacgcatc atgaagagaa
     7801 agcggcgcta acctgcggtg atggggcgtt tattcgtgac gaggctaaca ttacgctggt
     7861 tgccgattcc ccactgcgcg ctttgctgat agatttgcct gtctagttgt ttttacaggg
     7921 agatgatgat gagtaaaaaa tcgaccaaaa agcgccagcc agcgaagccc gtggtggcga
     7981 aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga gatgttgagc gagctggaag
     8041 ctatcgtcgc ggatgctgaa acgcgtttag ccgaggatga agctaccgcg taaagacagg
     8101 ttgccggatg cggcgtaaac gccttatccg gcctacaaaa cacaacaaca tcaacaaatt
     8161 aaacaccccg taggccggat aagatgcgcc agcatcgcat ccggcatcag gtacaaaaca
     8221 caacaacatc aacaagttga gcaccccgta ggccggataa ggtgcgccag catcgcatcc
     8281 ggcacaatac tcaaaacctg gcgtttatct ggccttgctc gccataatct cgataatctg
     8341 ccgatccgtt tgctgcatcg aatggcttgc taacgcacac aggttggcaa tcgactgctc
     8401 aacatcatgc gccacgatcc cttcattgcc ggtcacggcg gtatcatcca gcgccattaa
     8461 caccgctttc cacgcagccg aagcactggt cgaaaccttc atcgcgcagc tgttcgacgc
     8521 accatcgcaa atcatgccgc tgacatcgcc gatcatactg ctgatcgcca tcgagatagt
     8581 ttcataacgc ccatccacca gccatgccat cccggcggcg gcccccattg ctgcggtcgt
     8641 tgcggcacaa agtgcagaca aacgcggtaa ctggttatgg atgtaaattg cgctcaaatg
     8701 agaaagcatc agcgcacgcg ccagtcgttc atcatccgct ccgaagtgtt ctgctacaac
     8761 caccacaggc attattgcag tgatcccctg gttacccgag ccggagttac tcatagccgg
     8821 aagcgtagcg ccgcccatac gcgcatcgga tgccgcgctg gtacgaatca caatggatga
     8881 agagagatct ttcgccagca aaccgcgctc gcactgtttt tccagcgtcg cgccaatatg
     8941 cagcccccac ttaccgctca aaccttcctg cgataacgca caatttagct tcgcggaatc
     9001 gagaataaag cggatcgccg caaatgggac ttcattgacg aacttcagga tctcagccag
     9061 cgtcgttctg gaaagcaccg tcagcggcga ctcttgctcg ccctctgcca cacacgcctg
     9121 gtgggtaaac accacaccat tgtgcgtctc aatatgcaca atgttggtat gcccgccgac
     9181 gatggtgaca cacgcccact tctcaccgtt ccagactttg gcgcgtgaga agaggatttc
     9241 attgcaaggt tcctggatct taacagagac tttccccgcc gccagcagtg ctttggcatc
     9301 ggtaattgcc tgcgcagttg cgtctttcag cacttccagc ccggcgttgg catttccacc
     9361 taacgccccc agcgccgccg caatcggcag ccccaccatt cccgtgccgg gaacggtgac
     9421 gcccagaccg ttcttcatca gatttggcga aacccaggct tctacacgtt caaccggacc
     9481 ttccagttct gccgcagcaa ccgccgccgc cagcgccagt gaaatcggtt cagtacatcc
     9541 cagcgccggt tttacttcct cctgaacggc gaggatgtaa cgctgccata acggatttaa
     9601 agtcgaatca aacatattaa aaaccttaaa atttcaggta aatcaagaaa atgccaggaa
     9661 cagagaaaca caaagcaaca aaccagtgac gataatcagg tacagagaca tccctttgta
     9721 tttgtgcaat gccggtactt tgtaaaccag ccacgccggg atcaggcacc ctaccatgcc
     9781 gaaaatcggg ctacagatag aggtgaagct caacaccggt gcgttcagta cgatggcgct
     9841 ccaggccagc aaaatggcga aaatcatgat gccgcgctga acaagatttt cgttaatctt
     9901 ctcggcaggc atcttgcgac gcaggatgtt cattacgatc ccttgcgttg cttcgcgaaa
     9961 gcctaaatag acgccaaaga atgcagtcat gacagcaaag atattgagaa tgacgctgac
    10021 cactttcacc cacgctgcgc cgtcaccgct aataaactgt gcggcaatcg ccagcgcgga
    10081 aatattctgc tcgtaggctt ttaccgcttc gtcatgtccc atcgccagcg tgaacgacac
    10141 ggcgtagaaa aagacggtga caaacaaaat gccaaacgcg atattcattg cccgcaatgc
    10201 tttatgccgc gccacttcaa ttgatttttc ccgcgagcga taagagatca ccatcgggct
    10261 taacgtctgg ataaacagaa tcgacgtcag ggtaaacggc agcgtaataa tggcgttttt
    10321 caccagcagc cccagcggcg gtagcgaacc gacgttatac agatgccaca ttcccaccat
    10381 cgacacgccc agcgcagcga ctaccagcag cttggtcagc accatgtcgg tcgaaatttt
    10441 gaataacaat ttctcgccgc gtgaagagat cgccaccaga atgcaaatca gtaccagacc
    10501 ataaaaggga ctgtctgaca gcaacccttc cgtcacgccg aaggtatgca gataggaagc
    10561 actatcgttg gtgatggcgg tggaatagac gaacatccaa atcaccagca ttacgaaata
    10621 gagcgcacct aacaggatgc cccagttttt acctaaataa ccgctaatga cactcgggta
    10681 atctttacat tctggtgatt ctgccagcgt attaataaac aaccgctgaa acagatacat
    10741 tgccgggtaa ccaatcaccg atgagagcaa aaatacccac aatcccatca aaccgacctg
    10801 caccgggaga aaaacaatcc ccgcgccaat cgccatcccg atactcataa tcacccagcc
    10861 ggtgtcagta ctgtcgaatt tgatcgcttc tcgccactcg ctctcactca ttccagcacg
    10921 acccgccggg gtcgaagcgt ctgcaatgac gcctttattc gatgcaattt ccataatttc
    10981 tcgctcaata ttttgtaggg cttattattt ttttccgagc cgcatcaagg cgatatgcgg
    11041 tctacgtttt tgcaggcgag ttattagaat aaagaaatga tacgcgcgag gtcagagaaa
    11101 atcttcacaa tcaagacttt aaaataacgg agatggataa gaattttcta ctaaattaat
    11161 cacaagagaa tattcattct ctatttgcgg cagatcacaa aaaaaaggtg cacatttgtg
    11221 cacccaagga tgaaagctga cagcaatgtc agccgcagac cactttaatg gccagtcctc
    11281 cgcgtgatgt ttcgcggtat ttatcgttca tatctttgcc ggtttcatac atcgtctcga
    11341 tcactttatc gagtgaaaca cgcggtgccg aggtgcggcg catcgccatc cgcgcggcgt
    11401 ttactgcttt cacggcatta atggcattac gttcaatgca cgggatttgt acctgtccgg
    11461 caaccggatc gcaggtcagc ccaaggttat gctccatcgc gatttccgcc gcattgcata
    11521 cctgcgccgg actgccgccc agtagttcag ttaaccctgc cgctgccatt gaacaggcca
    11581 cgccaatctc cccctgacag ccgacctccg cgccagagat ggaggcgttc attttataca
    11641 gcgcgccaat agcccccgct gccagaaaat agcgggcaat tgaccgctcg tttaccggac
    11701 gacggaactt atcgtaataa gccagcactg ccggaataat gccgcacgca ccgttagtcg
    11761 gtgccgttac cacgcgcccg ccagctgcgt tttcttcact aaccgccagc gcgtacatgt
    11821 tgatccagtc gatgacattc atcggatcgt tagagatgtt atcgctagaa accagctgac
    11881 gacgcagcgc tacggcacga cacggcacat tgagcggacc aggcagcacg ccttcagtgt
    11941 tcatcccgcg ttcaataccg tcatgcatca cttgccagat acgggcaaac cctgcgtcaa
    12001 tttccgcttt gctgcgcagc gccagctcgt tgtgcatcat caggccagat atagacaggc
    12061 cgttgtaatc acacattttc agcagttcac ctgctgagtg gaaatcgtaa ggtacggacg
    12121 tttcgacatc gtgcgacagg ccgaagtgtt cttcttcgac aataaacccg ccgccgacag
    12181 agtaataggt tttacttaat agcgcttcct gtcccttcca ggcagtgatc cgcattccgt
    12241 tctcatggcg aggcaacatt tcgggatgga agataatgtt ctttgctaca ggaaaatcaa
    12301 caatatgcgc accagatgcc actggcagcc gcccgctgcg cgttactaac tctataaatg
    12361 cagggatctc atcaatgaca acatcctgcg gactgtttcc tgccagtccc atgataatgg
    12421 cgacatccgt ggcatggcct ttgcccgtca gtgacaacga cccgtacaga tcgaccacaa
    12481 tatggctcgt cgcggttaat aagccgctac tttccagccg atcaataaaa ctttttccgg
    12541 cattcattgg ccccacggta tgcgaactgg agggaccaat cccaattttg aaaatatcga
    12601 atgcactaat catatccaca ccctcggatt gccgttcagt gaagtggagc ggaacgacct
    12661 tgcgaccgtc ccgctcacga ggctttacgc actacgtact gcgatagctt caatttccag
    12721 cttcacatct ttcggcaaac gcgcaacctg cacacagctc cgggtcgggt aggtcgcctg
    12781 atgctcatcg aagaactgct tatagacttc attgatggtg gcaaaatcat tcagatcggt
    12841 gatgaacacg gtcatcttga tgatatcgcc cacgctcagc ccggcagcaa ccacgatcgc
    12901 tttgacgttt tcgaggctta aacgcgcctg atcttgcaca tcagccggga tctcaccggt
    12961 ctgcgggcaa accggaattt gcccggaggt gaagaccatg ctgcctaaat caacgccctg
    13021 aacataaggg ccgattgcgc ctggggcacg ttgcgtttcg ataatctttt tcatacatcc
    13081 tccggcgtca gagcgcctgg gtaaaagtac gtgaaataac atcctgttgc tgttcacggg
    13141 tcagtgcgtt gaagcgcacg gcgtagccag agacacggat tgtcaggtta ggatattttt
    13201 ccgggtgctc gatggcatcc agcagcattt cccgattcat tacgttgacg ttgaggtgtt
    13261 gaccgccttc gacatccgct tcgtggtgga aatacccatc cagcaggccg acaaggttgg
    13321 ttttacgtac tggatcttct ttgcccagcg ccgcaggaac gattgagaag gtgtacgaga
    13381 tcccatcttt ggcgtaggtg aacggcagtt tcgccaccga cgtcaatgag gccacggcac
    13441 ctttgcggtc acgaccatgc atcgggttag cgcccggcgc gaacggtgtt ccggcgcgac
    13501 gaccgtccgg cgtattaccg gttttctggc cgtacaccac gttagaagtg atagtcagaa
    13561 tcgactgggt agggacggcg ttgcgatagg ttggcagcgc tttaattttc ttcataaagc
    13621 gttcaaccag gtcgcaggca atgctgtcta cgcgctcgtc gttgttgccg tactgcggat
    13681 attcaccgtc gatttcaaag tccaccgcca ggccgttttc gtcacggatt ggtttcacgc
    13741 gggcatattt gatggcagac agggagtccg tcgccaccga caggcccgcg atgccgcatg
    13801 ccatagtgcg atagacatca cgatcgtgca gcgccatcag cgaagcttcg tagctgtact
    13861 tgtcgtgcat gtagtgaatg atattcagcg cgctgatgta ctgcaccgcc agccagtcca
    13921 tgaagtgatc gaggctgtcc atcactttgt cgtaatccag cacgtcgtcc atcagcggtg
    13981 ctgttttcgg cccgacctga atcttcagct tctcgtccac cccgccgtta attgcgtaga
    14041 gcagcgtttt cgccaggtta gcgcgtgcac caaagaactg catttgctta ccaatcacca
    14101 ttgggctgac gcagcaggca atcgcgtaat cgtcgctgtt gaagtcagta cgcatcagat
    14161 cgtcattttc atactgcaag gaagaggtga cgatcgacac ctgcgcggca tattttttga
    14221 aggcaatcgg taattcttcc gaccaaagaa tggtcaggtt aggttccggt gccggcccca
    14281 tagtgtgcag ggtgtgcaaa tagcggaagg agtttttggt caccagcgta cgaccgtcca
    14341 gccccatccc gccgatcact tccgtcgccc agattgggtc gccggagaac agcgaatcaa
    14401 attccggtgt acgcaggaag cgtaccatac ggatcttcat gatgaagtga tcgatcagtt
    14461 cctgtgcctg ctgctcattg aggacgccag ctttaaagtc gcgctcaatg tagatatcca
    14521 ggaacgatgc cgtgcgcccc agcgacatcg cgccgccatt ttgcgatttc actgccgcca
    14581 gataagcgaa gtagagccac tgcaccgctt cctgtgcatt ctgcgccggg cgagagatat
    14641 caaagccata tttcgccgcc atttcctgaa tctgcaacag cgcatgacga tgctctgcca
    14701 gctcctcacg cagacggatg gtggcttcca gatcctcgcc tttttccaga cgagactgga
    14761 gatcggcaaa ttgcagttcg cgttcacgca ccagataact gatgccatac agcgctacgc
    14821 ggcgatagtc accgataatg cgcccacgac catagccatc tggtaaaccg gtcagcacgc
    14881 cagatttacg gcagcgcagc atatccggtg agtaaacatc aaatacgccc tggttatggg
    14941 ttttacgcag atcggtaaac agatattcaa attcactgtc catttcacgg ccataggcgt
    15001 ggaatgaact tttaatcata ttaatgccac cgaacgggtg tagcgcacgt ttcaacggcg
    15061 catccgtttg caggccaaca attttttcca gcggctggtt aatatatccc gcatcatgag
    15121 cggtaattgt ggtggcaata ttggtatcga aatcaaccgg cgcgtgggtt gcgttttcga
    15181 tacggatgcc ttccattacc ttttcccaca attccgtggt ggcaggcgtc gcttcggcga
    15241 gaaaagattc atcgccttca tacggtgtat agttatgttg aataaaatcg cggacattaa
    15301 tttcgctttt ccagtccgta cctttaaagc caagccatgc gtcagcgtac agcttatcgc
    15361 tggtatcaat atctaccttc atgaaaaata atctctctac aatacttcaa ctaaattatg
    15421 caaattctgc gggcgcgtta actttaccta aatgaatggc atccaaagca atcatttttt
    15481 cttcgttagt cggaataacg acgcaaatga catgcgcatt ttcactggaa acaattcgct
    15541 cgccaaaaga gttagaacga ttattcattt ctgtatcaat ctctacgcct aatacagcca
    15601 aatgttccat gaccagacga cgaattaaac ttgaattctc tcctattccg ccggtgaata
    15661 taattccatc cagccgacgt aatgaagctg catgtccggc aatatgacgg gcaattcggt
    15721 gaacaaaggt tttaattgcc agttgcgcgc gttcgtgacc ttcatgccag gctttttcca
    15781 gaacacgtaa atccgaagaa agaccggaaa tacctaataa tcccgactct ttattcacta
    15841 cgcgttccag gtcacccagg ctctggttgg tttggctggc gacccaggac atcgcaccaa
    15901 agtcgacatc gccactgcgg gtacccatca tcaagccttc cagcggcgtc attcccattg
    15961 aggtgtcaac actctgaccg ttgcgaactg cgcagattga cgcgccattg ccaagatgcg
    16021 ccacaaccag gccggagtca tcttccgcca gattcagcag cgaatgtgcg cgctgggaaa
    16081 catagcggtg cgacgtgccg tggaaaccat aacggcgtac acctaactct tcataatatt
    16141 tccacggcag gccgtataaa taagcttccg gagccatcgt ctggtggaaa ctggtatcaa
    16201 ataccgccac ctgagttacg cccggaaata attgctgcgc cgattcaata ccacttaaat
    16261 ttgcgtaatt atgcaggggt gccagtggag aaacgcgacg gatattatca atgacttcat
    16321 cggtaataat ggcggactcg gtaaaaatac tgccgccgtg agcgatgcgg tggccaatta
    16381 aggccacgct gtcatttaaa ttccgttttt ccagttcaaa tgcaattgcc ttcaatgcac
    16441 cttcgtagct gtggtgagcc agcggtgctg gctctccccc atttacggat aagaatgcat
    16501 tttccgagtt aataccgtcg gcaatacctg acattaatac ttcacaatcg ctggcatcga
    16561 gtacggaaaa cttaatcgaa gacgaaccac agttaataac caaaacaacc ggaaattcat
    16621 tcatctcttt tctcatcctg agttacggat taaaacagtt tgtatacgat gttcaggatg
    16681 gtcagcagac caatcacggt aacaaacacg ttatccagac gaccacggta tttcgccaga
    16741 gacggcgctt tacggatagc atacatcggc aacaggcaca gcagggatgc gataatcggt
    16801 gcgcccatgg cttcaatcag gtcgaggatg ttcgggttgg cgtaggcaac aacccaggtg
    16861 gagcccatga tgaagatcat gctgagagta ttcagtttac ccagcgacac tttggttttg
    16921 tcacctttat aaccgaactt cagaatcaga ccattcaagc cttccagcgt ccccagatag
    16981 tgaccgaaga aagatttgaa gatagccacg agtgcgatga tggaagccgc atattccagt
    17041 gtaatcgcga acgttgtttt cgtaccggtc atggacgcaa agtggttagc cagataagaa
    17101 agcactggaa tattctgcgc tttggcttcc gccatgttgg ccggagacag agtaaacagg
    17161 cagctaaagg caaagaacat caccactgca accatcagca tgctggcacg agaaatgatt
    17221 tgggaacatt tacgttcggt gaagtcgcga ccgaagtctt tctcatactc ttcacgttta
    17281 gaaaccacga aggaagagac gattggcgag aagttaaagg agaaaaccat gatggaaatc
    17341 cccagccaga cagtgatcag gataccgtca tgaccggtta acgacagcga accgaggtca
    17401 acctggtcga taactgcaga gttccagtaa gggatcagcg acagagaaat cagcaccagg
    17461 ctggcgataa acggccatac caggtagctc atcactttaa ccatcagatc cttaccaaac
    17521 cagatgacga aagccatcag cagcaacagg aacagcgcca caaagccgcg attcagcggt
    17581 gcaaatccga gctggttttc ccagaacgtc ataaaggtat tggtaatagt aacgccataa
    17641 atccacagca gtgggcaaat cgcgaagaag tacaggaacg tgataaccac gccgccagtt
    17701 ttaccaaaat gctcttccac cgtttccgta atgttgccgg aagggttaga gccagaaaga
    17761 cacagacgcg ccagcgcccg gtggcaataa aatgcgatgg ggtatgccaa taccaacatc
    17821 agaagaatcg ggatcagtcc gccaaaacct gcgcggatag ggaagaacag caccccggcg
    17881 ccgattgccg taccaaacaa gcctaacgtc catgtggtat ctgatttacg ccaggacgat
    17941 tgttttgtct ggctggatac aatgctatct gaagtactca tatcctatcc tcaacgaatt
    18001 aattaagcgt caacgaaacc ggtgatttga gagacgcgag aaagatcgat attgccgccg
    18061 gaaataatac tgacggtttt tctattttga atatattggt ctaatttacc gcttaataat
    18121 gcagcacatg ccagagcgcc tgcgccttcg gtgacgactt tattgcgctg aattaaggca
    18181 atcatactgt ttctgatttc gtcttcgctg accagcacga tgtcatcgac taattcacga
    18241 acgatttcgt aagttaaatt acccgggcgg gagacatcac aaccatccgc cagggtgccg
    18301 gtagttcggt gcgtggttat ttctccggag tggaaagaag ccgccatgcc gtgaacgttt
    18361 tctgactgta cgccaataac acgaatggtc gggttaatag atttaattgc caccgcaata
    18421 ccagcaatta aaccaccacc accaattggc acaatcacgt tatcgacatc atagagatct
    18481 tccataattt ccagaccaat cgttccctgg ccagcaatca ctttcggatc atcgtaaggt
    18541 gggataaaaa tacgaccttc catttcgaca atttcgctca ctttagcgat agtgtcgttg
    18601 aagttatcac catgcagaac gacttctgcg gagtagtcgc acgttgccgc tactttggat
    18661 tttggcgcac cttttggcat caccacttta ccgtcgatac ccagcatcgc gcaggagagc
    18721 gaaacccctt gcgcatggtt gcccgcagaa caggccacca cgcctttgcg tttttccgca
    18781 tcggtcagtg aacttaattt attaaatgcg ccacgaattt taaatgaacc cgtacgctgc
    18841 atattttcaa acttcaggaa tatttcacct ttgcaacgtt cactaaaata gttggagcga
    18901 ggcatgcctg ttttataaat tcgcccagcc agtcgttgtt tcgcttcaat aatatcatca
    18961 atagcaaccg gcagatcgta tgtaatatgc attataaaac ctcttcgcct gaattaaata
    19021 tgtaggtaac cgtaaccgac acctgcaaaa cagacaggtg gattatttat gtttaaataa
    19081 aaacaaacaa ctaaccaact tctattaatt gccttcgtct acacccatta taagatgaat
    19141 actctttggc taattccacc aaaaccgatg ctgctttttt aatacgataa tttttcgacc
    19201 ataccgcggc atattgtgcc acaggtaatg tttcttcaac cggaatagta ataaattgat
    19261 tagaaccaaa aggtgacgtc atatcacaag gaattacagt taagaaatca gcattgagaa
    19321 caagattata aattgtcacg actgagtcgg ttttaacgat gttttcaata ctgatgccat
    19381 ttctttgtaa cgtagtaagc agttcgctgt agtaccccat attagtttgt ggcaacaccc
    19441 actgttcgtt cttcaacgac tccagcgtgg tggtgccggt gcatgttcgg gacttactgg
    19501 ctaccagcac aaactcggac tcgaacagcg gctcaacatg taaatcctga agcttcattt
    19561 ctgcacttaa cgtaccaatc gcaaaatcca gtcgaccgtc gcggattgcc ggtaagaacg
    19621 aagacagttg cgcttcatac atagaaacct gcgctttcgg gaacacctct ttgaacttgt
    19681 tgatcatccc tgacataaaa gtaaaaccaa tcaatgaagg aaaaccaaat gagacttcca
    19741 ccaccgcttc agaagacata ccgcttatct cattaaccat atttttcatt tcacgggtaa
    19801 tggattcgga acgggagagt aacaattgac cggcaggtgt taatgttaca ccggtatttt
    19861 tccgcaccac taattccaca ccaaaataat cttcaatatc gttaatgatt ttactgacgg
    19921 ccggttgagt taaccctaat tcttttgcag ccgagccgat agagccactt ctaatgactt
    19981 cctgaaagac taccaggtgc tgcgttttcg gaagaagaat agtgctcata acgacctacg
    20041 ttaattacct cattgacggc atgaagtgta tcaaaatgaa ataaacagga tatgtgcgac
    20101 cactcacaaa ttaactttca ttactttcca agg
//
LOCUS       DAZOYF010000067        20059 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZOYF010000067 DAZOYF010000000
VERSION     DAZOYF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.067"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            4..885
                     /gene="yieE"
                     /locus_tag="ACHUOF_002242"
     CDS             4..885
                     /gene="yieE"
                     /locus_tag="ACHUOF_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR0611409.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1091..2560
                     /gene="lysP"
                     /locus_tag="ACHUOF_002243"
     CDS             1091..2560
                     /gene="lysP"
                     /locus_tag="ACHUOF_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR0611410.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2852..4843
                     /gene="cirA"
                     /locus_tag="ACHUOF_002244"
                     /pseudo
     CDS             2852..4843
                     /gene="cirA"
                     /locus_tag="ACHUOF_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(4875..5710)
                     /gene="yeiG"
                     /locus_tag="ACHUOF_002245"
                     /pseudo
     CDS             complement(4875..5710)
                     /gene="yeiG"
                     /locus_tag="ACHUOF_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            5968..6636
                     /gene="folE"
                     /locus_tag="ACHUOF_002246"
     CDS             5968..6636
                     /gene="folE"
                     /locus_tag="ACHUOF_002246"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR0611411.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6653..7729
                     /gene="yeiB"
                     /locus_tag="ACHUOF_002247"
     CDS             6653..7729
                     /gene="yeiB"
                     /locus_tag="ACHUOF_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR0611412.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            7871..8911
                     /gene="galS"
                     /locus_tag="ACHUOF_002248"
     CDS             7871..8911
                     /gene="galS"
                     /locus_tag="ACHUOF_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR0611413.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9191..10189
                     /gene="mglB"
                     /locus_tag="ACHUOF_002249"
     CDS             9191..10189
                     /gene="mglB"
                     /locus_tag="ACHUOF_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR0611414.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10250..11770
                     /gene="mglA"
                     /locus_tag="ACHUOF_002250"
     CDS             10250..11770
                     /gene="mglA"
                     /locus_tag="ACHUOF_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR0611415.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11786..12796
                     /gene="mglC"
                     /locus_tag="ACHUOF_002251"
     CDS             11786..12796
                     /gene="mglC"
                     /locus_tag="ACHUOF_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR0611416.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12832..14045)
                     /gene="preA"
                     /locus_tag="ACHUOF_002252"
                     /pseudo
     CDS             complement(12832..14045)
                     /gene="preA"
                     /locus_tag="ACHUOF_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(14039..15277)
                     /gene="preT"
                     /locus_tag="ACHUOF_002253"
     CDS             complement(14039..15277)
                     /gene="preT"
                     /locus_tag="ACHUOF_002253"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFR0611417.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(15471..15710)
                     /locus_tag="ACHUOF_002254"
     CDS             complement(15471..15710)
                     /locus_tag="ACHUOF_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR0611418.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(15713..16432)
                     /gene="sanA"
                     /locus_tag="ACHUOF_002255"
     CDS             complement(15713..16432)
                     /gene="sanA"
                     /locus_tag="ACHUOF_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR0611419.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(16582..17466)
                     /gene="cdd"
                     /locus_tag="ACHUOF_002256"
     CDS             complement(16582..17466)
                     /gene="cdd"
                     /locus_tag="ACHUOF_002256"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR0611420.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(17596..18291)
                     /locus_tag="ACHUOF_002257"
     CDS             complement(17596..18291)
                     /locus_tag="ACHUOF_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR0611421.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(18288..18686)
                     /locus_tag="ACHUOF_002258"
     CDS             complement(18288..18686)
                     /locus_tag="ACHUOF_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR0611422.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            18925..19872
                     /gene="dusC"
                     /locus_tag="ACHUOF_002259"
     CDS             18925..19872
                     /gene="dusC"
                     /locus_tag="ACHUOF_002259"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR0611423.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         4915 a         4946 c         5260 g         4938 t
ORIGIN      
        1 actatgcaca tcaccctccg gcagttggaa gtttttgcag aagtattgaa aagtggatca
       61 accacccagg cgtcggtgat gctggcgttg tcgcaatcag cagtgagcgc agccttgact
      121 gacctggaag ggcagcttgg cgttcaactg tttgatcgcg tggggaaaag actggttgtt
      181 aatgaacacg ggcggctgct ctatccgcga gcgttggcac tgcttgaaca ggcggttgaa
      241 atcgaacaac tgtttcgcga agacaacggc gcgattcgta tctatgccag tagtaccatc
      301 ggtaactaca ttctgcctgc agttatcgcc cgttatcgcc atgattatcc gcagttgccg
      361 attgaactta gcgttgggaa cagccaggac gtgatgcaag cggtgctgga tttccgcgtt
      421 gatattggct ttattgaagg accgtgccac agcactgaaa tcatttctga accgtggctg
      481 gaagacgagc tggtggtttt cgccgcgccg acttcgccgt tggctcgtgg tccggtcacc
      541 ttagaacagc tggccgctgc gccgtggatc ctgcgtgaac gcggttccgg cacgcgggat
      601 attgtcgatt atctgttgct gtcacattta ccgaagtttg agatggcgat ggaattaggt
      661 aactccgagg caatcaaaca tgcggtgcgt catgggttgg gaattagttg cctgtcgcga
      721 cgtgtgattg aagatcaatt gcaggcaggc acattaagtg aagttgcggt ccctctgccg
      781 cgcctgatgc gtacgttgtg gcgtatacat catcggcaaa aacacctttc caacgcgcta
      841 cggcgctttc tggactattg cgatcccgca aatgtgccgc gttaagttgc tgtacaagaa
      901 catgctggtg ctgtgtcgat ttcgtgacgc agcgccttca gcatgcattc gccagaaaag
      961 agattggctg ctttacttat aatccctggg cgatcatgaa ggtgtcttat aaccgtgtat
     1021 ttctgccgga aggattgcca atcgtctgct acaatcgcgc catcattttt aagatggata
     1081 gcatttttgt atgggttccg aaactaaaac tacagaagcg ccgggcttac gccgtgaatt
     1141 aaaggcgcgt cacctgacga tgattgccat tggcggttcc atcggtacag gtctttttgt
     1201 tgcctctggc gcaacgattt ctcaggcagg tccgggcggg gcattgctct cgtatatgct
     1261 gattggcctg atggtttact tcctgatgac cagtctcggt gaattggctg catatatgcc
     1321 ggtttccggt tcgtttgcca cttacggtca gaactatgtt gaagaaggct ttggcttcgc
     1381 gctgggctgg aactactggt acaactgggc ggtgactatt gccgttgacc tggttgcagc
     1441 acagctggtc atgagctggt ggttcccgga tacaccgggc tggatctgga gtgcgttgtt
     1501 cctcggcgtt atcttcctgt tgaactacat ctctgttcgc ggctttggtg aagcggaata
     1561 ctggttctca ctgatcaaag tcacgacagt tattgtcttt atcatcgttg gtgtgctgat
     1621 gattatcggt atcttcaaag gcgcgcagcc tgcgggctgg agcaactgga caatcggcga
     1681 agcgccgttt gctggtggtt ttgcggcgat gatcggcgta gctatgattg tcggcttctc
     1741 tttccaggga accgagctga tcggtattgc tgcaggcgag tccgaagatc cggcgaaaaa
     1801 cattccacgc gcggtacgtc aggtattctg gcgaatcctg ttgttctatg tgttcgcgat
     1861 cctgattatc agcctgatta ttccgtatac cgatccgagc ctgctgcgta acgatgttaa
     1921 agacatcagc gtcagtccgt tcaccctggt gttccagcac gcgggtctgc tctctgcggc
     1981 ggcggtgatg aacgcagtta ttctgacggc ggtgctgtca gcaggtaact ccggtatgta
     2041 tgcgtctact cgtatgctgt acaccctggc gtgtgacggt aaagcgccgc gcattttcgc
     2101 taaattgtcg cgtggtggcg tgccgcgtaa tgcgctgtat gcgacgacgg tgattgccgg
     2161 tttgtgcttc ctgacctcca tgtttggcaa ccagacggta tacctgtggc tgctgaacac
     2221 ctccgggatg acgggtttta tcgcctggct ggggattgcc attagccact atcgcttccg
     2281 tcgcggttac gtattgcagg gacacgacat taacgatctg ccgtaccgtt caggtttctt
     2341 cccactgggg ccgatcttcg cattcattct gtgtctgatt atcactttgg gccagaacta
     2401 cgaagcgttc ctgaaagata ccattgactg gggcggcgta gcggcaacgt atattggtat
     2461 cccgctgttc ctgattattt ggttcggcta caagctgatt aaaggaactc acttcgtacg
     2521 ctacagcgaa atgaagttcc cgcagaacga taagaaataa gtttcctccc ttccttgcta
     2581 agccctctca accgagaggg ctttttcaat tccatttccc tgacaaatca tgcggatata
     2641 aaatttaaca tttggattga taattgttat cgtttgcatt atcgttacga cgcaatcaaa
     2701 aaaggctgac aaatcagagg ctgttccggc tttctgggat gatcacctgc atacaaataa
     2761 gtccaccgcg atgctgccgt acgcaagggg acgtaaagaa gatgtgagcg atacccattt
     2821 tattttcgta gttacctcat ggagatatgg aatgtttagg ttgaaccctt tcgtacgggt
     2881 cgggctgtgt ttgtccgcta tttcttgtgc atggcctgtg ttagcggtcg atgatgatgg
     2941 cgaaacgatg gttgtcactg catcttccgt ggaacaaaat cttaaagatg cacccgccag
     3001 tatcagcgtc attacccagg aagacctgca gcgaaaaccg gtacagaatc tgaaggatgt
     3061 cctcaaagaa gtgcctggcg tacaactgac gaacgaaggg gataaccgta agggcgttag
     3121 tattcgtggt ctggacagca gctacaccct gattcttgtc gacggtaaac gcgttaactc
     3181 ccgcaatgcc gtcttccgcc acaatgattt cgatctgaac tggatcccgg tcgattccat
     3241 cgaacgtatt gaagtggttc gtggcccgat gtcgtcgcta tacggctccg atgcgctcgg
     3301 cggtgtagtg aatatcatca ccaaaaaaat cggtcagaaa tggtcgggta ccgttaccgt
     3361 cgataccacc attcaggaac atcgcgatcg cggtgatacc tataacggtc agttctttac
     3421 cagtggacca ttaattgatg gtgtgctggg aatgaaagct tacggcagcc tggcaaaacg
     3481 tgaaaaggat gacccgcaaa actcaacgac caccgatacc ggagaaacgc cgcgtattga
     3541 aggattctcc agccgcgacg gcaatgtcga atttgcctag acaccgaatc aaaatcacga
     3601 ttttactgcc ggatacggtt tcgaccgtca ggatcgtgat tccgattcgc tggacaaaaa
     3661 ccgcctggaa cgccagaact actgcgtcag ccataatggg cgttgggatt acggcaccag
     3721 cgaactgaaa tcctacggtg agaaagtcga gaacaaaaac cctggcaaca gcagcccgat
     3781 aacttccgaa agcaatacgg tcgacggcaa atacacgttg ccgctgacgg cgattaatca
     3841 gtttctcacg gttggcggtg aatggcgtca cgacaaactt agcgatgcgg tgaacctgac
     3901 cgggggaacc agctccaaaa cgtctgccag ccagtacgcg ctgtttgtgg aagatgaatg
     3961 gcggatcttc gagccgctgg cgctgacgac cggcgtgcgt atggacgatc acgaaaccta
     4021 cggtgaacac tggagtccgc gtgcctacat ggtttataac gccaccgaca ccgtaacggt
     4081 gaaagggggc tgggcgacgg catttaaagc accttctctg ttgcaactta gccctgactg
     4141 gacgagcaat tcctgccgtg gcgcatgtaa gattgtgggt agcccggatc tgaaaccaga
     4201 aaccagcgaa agttgggagc tggggcttta ctacatgggt gaagaaggct ggctggaagg
     4261 gggtgaatcc agcgttatcg ttttccgtaa cgatgtgaaa gatcgtatca gcatcagccg
     4321 tacgtctgac gtcaacgctg caccgggcta ccaaaacttt gttggttttg agacgggcgc
     4381 taacggacgg cgcataccgg tatttagcta ctacagcgtt aacaaagctc gtattcaggg
     4441 cgtggaaacc gaactgaaaa ttccgttcaa cgatgaatgg aaactgtcga tcaactacac
     4501 ctacaacgat ggtcgtgatg tcagcaacgg cgaaaacaaa ccgctatccg atctgccgtt
     4561 ccatactgct aacggtacgc tggactggaa accgctggcg ctggaagact ggtcattcta
     4621 tgtttctggt cactataccg ggcagaaacg cgccgacagc gcgacggcta aaacaccggg
     4681 cggttatacc atctggaata ccggcgcggc ctggcaggtg actaaagacg tcaaactgcg
     4741 cgcaggcgtg ctgaaccttg gcgacaagga tctcagtcgt gacgactaca gctataacga
     4801 agacggacgt cgttacttta tggcagtgga ttatcgcttc tgatgagaag atgcccggcg
     4861 aaccgggcgg actttcactt cagtaaatac tgcgcatgga agcgcaggtg atcctctata
     4921 aaagaggcga tgaagtagta actgtgatca tatcccggct gaatacgcag cgtcatcggc
     4981 cacgctttct ggcgcgcggc ttctgccaat accgcaggtt gcaactggtc ggcaagaaac
     5041 tgatcattat cgccctgatc gataagcgtc gggatcgcat cctgcgcgtt actggcatac
     5101 atcagcgcgc aactatccca ttccagcatg catttttgtc ctcacctaaa taggtgctaa
     5161 acgctttgat tccccacggg acgctgcacg gattcacaat gggcgcaaag gccgaaacgc
     5221 tggtgtattt acccggattt ttcagcgcca taatcagcgc accgtgacca cccattgagt
     5281 gaccgctaat ggcgcagcgg tcgctgacat taaattgcga ctgaaccagc gccggtaatt
     5341 catcgcgcag ataatcatac atccggtaat gcgtcgccca cggcggttgc gtggcattaa
     5401 gataaaagcc tgcgccctgg cctaaatcgt agccatcgtc gttggcaacc ttttcgccgc
     5461 gcgggctggt gtctggcatc accagtacaa tccccagttc cgccgctacc cgctgggcac
     5521 ccgccttggt ggtgaagttc tcgtcattgc aggttaatcc ggaaagccag tacagcactg
     5581 gtggcggggt gtgatcacga ggggggggga gaaagatact gaacgtcatc gggcagttta
     5641 aggtactgga gtcgtgtcgc cagcgttgct gccagccttc aaaacagcgg tgctcttcga
     5701 gcatttccat gcgtggctcc ttgttgtgtt gtttgcaatt tcttcatcat accgtttatt
     5761 caatgtgctg tgagtaactt tcacttccgt atttgcataa cgatgtttta acatctgctg
     5821 atgaaaggca gcggcaatta caataattat cgctgtgaat actggattat gtgcgccgcc
     5881 tcacgcacaa taatcaagct gtaaatcagc ttaataactt tgcccccacg cagggtggag
     5941 gcgtcacacc tgcaggagaa atcataaatg ccatcactca gtaaagaagc ggccctggtt
     6001 catgaagcgt tagttgcgag aggactggaa acaccgctgc gcccgcccgt gcatgaaatg
     6061 gataacgaaa cgcgcaaaag ccttattgct ggtcatatga ccgaaatcat gcagctgctg
     6121 aatctcgacc tggctgatga cagtttgatg gaaacgccgc atcgcatcgc taaaatgtat
     6181 gtcgatgaaa ttttctccgg tctggattac gccaatttcc cgaaaatcac cctcattgaa
     6241 aacaaaatga aggtcgatga aatggtcacc gtgcgcgata tcactctgac cagtacctgt
     6301 gaacaccatt ttgttaccat cgatggcaaa gcgacggtgg cttatatccc gaaagattcg
     6361 gtgatcggtc tgtcaaaaat taaccgtatc gtgcagttct ttgcccagcg tccgcaggtg
     6421 caggaacgtc tgacgcagca aattcttatt gcgctacaaa cgctgctggg caccaataac
     6481 gtggctgtct cgatcgacgc ggtgcattac tgcgtgaagg cgcgtggcat ccgcgatgca
     6541 accagtgcca cgacaacgac ctctcttggt ggattgttca aatccagtca gaatacgcgc
     6601 cacgagtttc tgcgcgctgt gcgccatcac aactgattaa aaggcaggaa ccatggagcg
     6661 caacgtcacg ctcgattttg ttcgcggcgt cgccattctg gggatcctgc tattaaacat
     6721 cagcgccttt gggctaccaa aggcggctta tctcaatccc gcctggtacg atgctattac
     6781 gccgcaggat gcatggacct gggcatttct cgatctcatc ggccaggtga aattcctcac
     6841 gctttttgcg ctgctgtttg gtgctggcct gcaaatgttg ctgccccgtg gcagacgctg
     6901 gatccagtcg cggttaacgc tgttagtctt gttgggcttt attcacggtt tattgttctg
     6961 ggacggcgat attctgctgg cttacgggct ggtggggctt ggcgttttgc tgttattggg
     7021 gctgatttcc gacagccaga ccagccgcgc ctggacgccg gatgcatcgg ctattttgta
     7081 tgaaaaatac tggaagcttc acggcggcgt tgaagcgatc agtaatcgtg ccgatggtgt
     7141 tggcaacagt ttactggcac tgggcgcaca gtatggctgg caactggcag ggatgatgct
     7201 cattggtgcg gcgctgatgc gcagcggctg gctgaaaggg cagttcagct tacgtcacta
     7261 tcgtcgtact ggttttgtgc tggtggcgat tggggtgacc attaaccttc ctgccatcgc
     7321 cctgcaatgg cagctggact gggcgtatcg ctggtgtgcc ttcttacttc aaatgccgcg
     7381 ggaactgagt gcgccgtttc aggcgattgg ctatgcgtcg ctgttttatg gcttctggcc
     7441 gcaattgagc cgctttaagc tggtgcttgc gatcgcctgc gtcggacgga tggcgctgac
     7501 caactatcta ttgcaaacgc tgatttgtac cacgcttttt taccacctcg gtttgtttat
     7561 gcattttgac cgcctggagc tgctggcgtt tgttattccg gtatggctgg cgaatatcct
     7621 cttctctgtt atctggctgc gttacttccg ccaggggccg gtggaatggc tctggcgtca
     7681 gttaactttg cgtgctgccg gaccggcaat atctaaaaca tcaagataac gatctggatt
     7741 acattcgtta acaaaacggc tgtaaccgtt tccattgctg tgactcgatt cacgaagtcc
     7801 tgtattcagt gctgacaaaa tagccgccag caagcagtca tttactgcaa tctcataaca
     7861 ggtagtgaat atgatcacca ttcgtgatgt agcgcgtcag gctggcgtct ctgtggcaac
     7921 ggtttcccgg gtgctcaata acagcacgct ggtcagtgcc gacacgcgtg aagcagtaat
     7981 gaaagccgtg agtgagctgg attatcggcc aaacgccaat gcccaggcgc tggcaactca
     8041 ggttagcgac accattggcg tggtggtgat ggacgtttct gatgcgtttt tcggcgcgct
     8101 ggtaaaagcg gtggatctgg tcgcccagca gcatcagaaa tacgtgctaa tcggcaatag
     8161 ctatcatgaa gcggaaaaag agcgtcacgc cattgaggtg ttaattcgcc agcgttgtaa
     8221 tgcgttgatt gttcactcaa aagcattgag tgacgatgaa ctggcgcaat ttatggataa
     8281 cattcccggt atggtgttaa tcaaccgcgt tgtgccgggg tacgcccatc gttgcgtttg
     8341 cctggataat ctcagcggtg cccgaatggc gacgcgcatg ttgctgaata acggtcatca
     8401 acgtattggt tatctttctt ccagccacgg cattgaagat gacgccatgc gtaaagcagg
     8461 ctggatgagt gcgttgaaag agcaggatat tattccgccg gaaagctgga ttggcactgg
     8521 tacgccggac atgccgggcg gtgaggcggc gatggttgaa ctgctggggc gcaatctaca
     8581 acttaccgct gtatttgctt ataacgacaa tatggccgct ggcgcactga cagcattaaa
     8641 agataatggc attgcgattc cgttacatct ctcaatcatc ggtttcgatg atattcccat
     8701 cgcccgttac accgacccgc aattaacgac cgtgcgttat cccattgctt caatggctaa
     8761 attagccacc gaactggcct tgcagggggc agcaggcaat attgatcctc gtgccagcca
     8821 ctgttttatg ccgacgttag tgcgtcgcca ttctgtcgca acgcgccaga atgcggcggc
     8881 gatcactaac tcaacaaatc aggcgatgta accgctttca atctgtgagt gatttcacag
     8941 tatcttaaca atgtgatagc tatgattgca ccgttttaat gttgtaaccc gtatgtaaca
     9001 gtgaataatc actttcaccg aggtaatagc gtcataacaa caattaaagc cgttttctgg
     9061 agcgttaccg ggcatggaag aacgaatttt aaaaagtgag cttcggcgtt cagtaacact
     9121 tcattaactc tactgccccg ccgagcattt atctcaagca ctaccctgca taagaaaaac
     9181 cggagatacc atgaataaga aggtgttaac cctgtctgct gtgatggcca gcatgttatt
     9241 cggtgccgct gcacacgctg ctgatactcg cattggtgta acaatctata agtacgacga
     9301 taactttatg tctgtagtgc gcaaggctat tgagcaagat gcgaaagccg cgccagatgt
     9361 tcagctgctg atgaatgatt ctcagaatga ccagtccaag cagaacgatc agatcgacgt
     9421 attgctggcg aaaggggtga aggcactggc aatcaacctg gttgacccgg cagctgcggg
     9481 tacggtgatt gagaaagcgc gtgggcaaaa cgtgccggtg gttttcttca acaaagaacc
     9541 gtctcgtaag gcgctggata gctacgacaa agcctactac gttggcactg actccaaaga
     9601 gtccggcatt attcagggcg atttgattgc taaacactgg gcggcgaatc agggttggga
     9661 tctgaacaaa gacggtcaga ttcagttcgt actgctgaaa ggtgaaccgg gccatccgga
     9721 tgcagaagca cgtaccactt acgtgattaa agaattgaac gataaaggca tcaaaactga
     9781 acagttacag ttagataccg caatgtggga caccgctcag gcgaaagata agatggacgc
     9841 ctggctgtct ggcccgaacg ccaacaaaat cgaagtggtt atcgccaaca acgatgcgat
     9901 ggcaatgggc gcggtagaag cactgaaagc acacaacaag tccagcattc cggtgtttgg
     9961 cgtcgatgcg ctgccagaag cgctggcgct ggtgaaatcc ggtgcactgg cgggcacagt
    10021 actgaacgat gctaacaacc aggcgaaagc gacctttgat ctggcgaaaa acctggccga
    10081 tggcaaaggt gcggctgatg gcaccaactg gaaaatcgac aacaaagtgg tccgcgtacc
    10141 ttatgttggc gtagataaag acaacttggc tgaattcagc aagaaataag actgattggg
    10201 cgcagtcgtt tactgcgccc ttattgtatg gccaacaagg taaaattata tggtcagctc
    10261 aacgactccg tcttccgggg aatacttgtt ggaaatgagc ggtatcaaca agtcctttcc
    10321 tggtgttaag gcacttgata acgttaattt aaaagtccgg ccacattcta tccatgcatt
    10381 aatgggggaa aacggtgcag gaaaatcgac attattaaaa tgcctgtttg gtatttatca
    10441 aaaagactcc ggcaccattt tattccaggg taaagagatc gatttccatt ccgccaaaga
    10501 ggcactggaa aatggtattt cgatggtaca ccaggagtta aacctggtat tacaacgttc
    10561 ggtgatggat aacatgtggc tggggcgata tcccaccaaa ggcatgtttg tcgatcagga
    10621 caaaatgtac cgcgaaacca aagcgatttt tgatgaactg gatattgata tcgatccgcg
    10681 tgcgcgcgtc ggcacattat ccgtttcgca aatgcagatg atcgaaatcg ccaaagcgtt
    10741 ttcctataac gcgaaaattg tgattatgga tgaaccgact tcttcgttaa ccgaaaaaga
    10801 ggtcaatcat ctgttcacta ttattcgtaa attaaaagag cgcggctgcg gtattgttta
    10861 tatctcgcat aaaatggaag aaatcttcca gttatgtgat gaagttaccg tattgcgcga
    10921 cggtcagtgg atcgccaccg aaccgctggc aggactgacg atggacaaga tcatcgccat
    10981 gatggttggg cgttctctta atcagcgttt tcctgacaaa gaaaacaagc cgggcgaagt
    11041 catcctcgag gtgcgtaacc taacgtcact gcgccagccg tcgattcgcg atgtctcgtt
    11101 tgatctgcat aaaggggaga tcctcggtat tgccgggctg gtgggggcga aacgtaccga
    11161 tattgttgag acgttatttg gtattcgcga gaaatcggct ggcaccatta cgttgcacgg
    11221 caaacagatc aataaccata atgccaacga agccataaac cacggatttg cactggtaac
    11281 tgaggagcgc cgctcaacgg gaatttatgc ctatctggat attggtttta actcgttaat
    11341 ttccaatatt cgcaactaca aaaataaagt tggtttactg gataactcgc ggatgaaaag
    11401 cgatacccag tgggtgattg attcgatgcg ggtaaaaacg ccgggtcatc ggacgcaaat
    11461 tggttcgctc tccggtggta atcagcaaaa ggtgattatt ggtcgctggc tactaacgca
    11521 accagaaata ttaatgctcg atgaaccgac gcgcggtatt gatgtcgggg cgaagtttga
    11581 aatttatcag ttaattgccg aactggcgaa gaaaggcaag gggattatta ttatctcctc
    11641 tgaaatgcct gagttgttag ggataacaga ccgtattctg gtcatgagca atggtctcgt
    11701 ttccggaatt gtcgatacaa aaacaacaac gcaaaacgaa attctgcgtc ttgcgtcttt
    11761 gcacctttaa gattaggggc ttcccatgag tgcgttaaat aagaaaagtt ttcttactta
    11821 cctgaaagag ggcggtattt acgtcgttct tttagttttg ctggcgatta ttattttcca
    11881 ggacccaaca tttttaagtc tgttgaactt aagtaatatt ctcacccagt catcggtgcg
    11941 tattattatc gcgctcggtg tggcagggtt aattgtcacc caggggaccg atctttctgc
    12001 tggtcgtcag gtagggctgg cggcagtggt ggctgcgaca ttattgcagt ccatggataa
    12061 cgccaacaaa gtgttcccgg aaatggcgac gatgccgatt gcgctggtta ttctgattgt
    12121 ctgtgccatt ggtgcggtga tcggtttgat caacggtctg attatcgctt atctcaacgt
    12181 gacgccgttc attaccacgc tcggcacgat gatcatcgtc tatggcatca actcgctcta
    12241 ttacgacttt gtcggggcgt cgccaatttc tggttttgac agtggcttct ctacctttgc
    12301 tcagggcttt gtcgcgctgg ggagtttccg tctctcttac atcaccttct acgcgttgat
    12361 tgcggtggcg ttcgtctggg tgttgtggaa caaaacccgc ttcggtaaga acatttttgc
    12421 cattggcggt aacccggaag cggcaaaaat atctggtgtc aacgtcggcc tgaacctgct
    12481 gatgatctac gcgttgtctg gcgtgttcta tgcctttggc gggatgttag aagccggacg
    12541 tatcggctct gccaccaaca acctcggctt tatgtatgag ctggatgcta tcgcggcgtg
    12601 cgtggtaggc ggtgtatcgt tcagcggcgg tgtggggacg gtgattggcg tggtgaccgg
    12661 ggtaattatt tttaccgtca tcaactatgg cctgacgtat atcggcgtaa acccatactg
    12721 gcagtacatc atcaaagggg cgattattat cttcgccgta gcgctggatt cactgaaata
    12781 cgcgcgtaag aagtgatgtg attgcggata ttgccggatg cgtctctggt tttacaacgt
    12841 taccgggtgt tctttctcgc ctttcttaaa cttcacttcc ccgagctcaa tacaacccac
    12901 cgggcagacg tgaccacaaa gcagacaacc cacacatttc tcggtattac aatgcggtgt
    12961 gcgggttttc tcgctccatt ccatcgcctg gtgaccgccg tcgtaacagg aaatataaca
    13021 gcgtccacag ccaacacatt tgtcaagatt gatacgggga tagacaatat aactgcggtc
    13081 taaatcttcc gccgggacaa tattgttatt cgccagacct accatctcct gcaacgaatc
    13141 aaatccctga tcggcgagat aatgcgataa cccgctcgcc atatcttcta cgatgcgata
    13201 gccgtactgc ataatgccgg tggtgacctg taacgttgct gcgccgagca ataaaaactc
    13261 cgccgcatct tcccaggttt caatgccgcc gataccgctg attgggaaat cgcgcagttc
    13321 tggatgggtg cgcatttgct gaatgaagcg cagggcgatc ggttttaccg ctttaccgga
    13381 atatccggaa atactcgatt taccgttaac gatcggcata ccgattttct gattaagatc
    13441 gatattggtg atggatttaa cggtgttaat tgccgcaatg ccatctgcgc cgccgcgctt
    13501 cgccgccagc gccacttcgc acatatcacc gatattcggc gtcatcttcg ccagcattgg
    13561 cagcgtggaa ccccgtttca ctgcccgaca atatttttct accagctccg ggctttgccc
    13621 gacatcgcta cccatcgcat gagaagtcat ttgcggacag gagaagttac actcgatcat
    13681 atccgcgcca gctgctccca ttgctgctca ttttccccca tgatcgaagc gatcaatact
    13741 ttgtccgggt aatcttcctt cagccgacgc agggcggtca gattctcttc caacggatgt
    13801 tcagcaatct gctccatatt tttgaagccg ataaaaccgg tatcttcttt caccagatga
    13861 tcaaaacgcg gcgagacttc gttggcgata aaaaagccga tcgttttaaa caccacaccg
    13921 ccccaacctg tgtcgtaggc tttggcacac atctcatagc agttgcctac cggcgaagaa
    13981 gagaggcaga acgggttggg aaagttcacg ccgcaaaaag taatcgaaag atctttcgtt
    14041 aacatgagca agctccctct aaatagtgat gaatcgcctc ggcggcttct ttcccggttt
    14101 tcacggcata gaccacggtt ttgtcaccct caacaatatc gccagcagca aagacttgcg
    14161 gatcgcggat ctgataattt tgtgttttga tggtgttacg ctgctgctct aactccgcaa
    14221 aggcatccag tctggcatgc tgaccgacgg cgagaataat tttatctgcc gccatcgtca
    14281 gttcgcctga taaccgtaca tgcttaaacg tgactttatt gccttccacg gctactggcg
    14341 tgaatccatc gatgatcgaa acgcccagct cccgggcgct ggcaaactct ttttcgcttg
    14401 ccggaaactc atctaactct tcacgtgcta cgcaagttac cgcctgacag ccgagaactt
    14461 tcagcgtgct ggctacgtcc atcgcgacat caccgccgcc gataattaat gcgctttgtg
    14521 gaatgctgat atcgccttgc gcctgtcgta cacgttgcag gaagtcgacg gcaatctcaa
    14581 cgtcgctatg ctcaaacagc ggtagcccgg aaccgcttga taaccccacg gtgaccagta
    14641 ccgcgcggtt ttccgctttc agttgctcca gggtgagtgt tttaccgatt tcgttattgc
    14701 acttaatggt cacgcccatt ttttcaatac gggcgatctc tgcatccagc actgattgcg
    14761 gtaaccggaa ttgcggaata ccgttacgca accagccacc ggggtgcgct tctttctcat
    14821 aaatcgtgac gtcataaccc tggtttgtca gtgtcacact ggcctgcaat ccggcaggac
    14881 ctgcgccaat aatcgcgact ttgccgagcg ttttagtacc gggctgataa atttccatcc
    14941 cggtttgttg ttcaaaatca gtaacaaaac gctgtaagcg gccgatatca atgggagcat
    15001 caacaccggc acgggtacaa ccgctttggc ataatttctc cgtcgggcac actctggcac
    15061 aaacggcacc gagggcatta ttttcgcgaa ttgtctcggc agcgccttta aaattacgaa
    15121 agtagattga gcgaataaat ttccccggat cggtctgtgc cgggcaagcc tgactacagg
    15181 gagcgtcgtg acataataaa cagcgagagg cttctttaat cgccagtaac ggcgtaaaag
    15241 ccggagtgag ttcatccaga taattttgtt gcggcatgtg atcttcctta atgctatgag
    15301 taattatggc gaacgctaaa taaatattgc gtgctgatat taaggctatg cgcgtgggga
    15361 aatttaagct gtgaaaggat tcacaaatag ctgcggttca gaatgttata tccgcagctt
    15421 attgagtgaa gtaataatta ttctcaataa ttaacagaaa agagaaaata ttaacgatag
    15481 aaaataagat aaacaatcat gccgatagat aaaattccga agccaatata tgccaccata
    15541 taagcgaaga agagtccggg attttttgct cgccagacat acaccggttc cggtgcattt
    15601 ttaacccgtt tatcaatcgc gcctgcgcgc cagcctttcc atgcttcgcg gaaacaaaat
    15661 atcgggataa ggaaaaaaac ggcaacgaca aaaaactgct gtacatccat aactactttc
    15721 cttgtttctt ttgtaattca agtaactgtt cgggtgtaac ggcgggatac ccctgcgcat
    15781 cttccggcac ctggtgcata gccggaatag ggaccagcgg ccctaaaaaa cgcggttcac
    15841 gtttaaaaat ataaaggtca gccagcgcac cgaaacgggc ggcaaattcg cgaatacgta
    15901 ctgacagcat atctttcggt gacggtacgg cataacactg agcctgaatc cccatatgca
    15961 gcgcaataaa taatgctcgc tcacagtgga aacgttgggt gataataatg aaatcattag
    16021 tatcgaaaac tttgcgtgta cgcacgatgg aatccagcgt acgaaagcct gcgtaatcga
    16081 gaacaatatc tgatgggtcg acaccagcag cgattaaatc tttgcgcatg gtcatcggct
    16141 cgttataact ttgcaatgcg ttatcgccgc tcagtaatag ataatttacc ttaccgctgt
    16201 tataggcatt aatcgctcct tgaatgcggt agcgataata ctgattaata acgccggtac
    16261 gataatattt tgctgtgccg agcaccacgc cgacctggcg gtaggggaga tcctgcaatt
    16321 cgtcgtagat ataaggcgcg gttttccagc tcatccagcg atcgaggccg agcacagtca
    16381 acagcagcaa gccgatcagg actaacaggc tgaggaacac gcgctttaac atgcaggaga
    16441 ctcgagctaa ggaaaatttc catcaggcta ctttacccgc tggttttgcg caagaaacgg
    16501 caaactgttt gccgggaatt tcgacaccag gagagggctg gcaagccgga caaggcgttc
    16561 acgccgcatc cggcaccagg cttaagcgag aagcacgcgg tcgatactgt gacagccgag
    16621 agctttcagc gttgcggagg tggcatccca ctgaatcaac ggcgcatcgg ctttttctgc
    16681 cagaaccgcg cgctggatat ccgggtaatc ataacccttg agattcaaca gaattaacgc
    16741 cccctgcaac ggcggcagag tcgggttgaa tgcggcgttt tcagcgtagc tgccgctgaa
    16801 aatacgaccg tctttacatt ccagcgcgac accgcttggt gacttactgt aaggcatgtg
    16861 cgaacggttt gccgctgcaa tcgccgcctg agaaagcgca tcaccagtca gcgcatagcc
    16921 gtgatcctgc tcgtccatca gcagcgtttt aatctccaga tctttcggcc caaaggcatc
    16981 cggcagatag tcacgcagcg cgtgtgcctc gcggcccggc aaatgaatac gcagatccag
    17041 accgctgttc agttcattca taaactgacg gcagtgacca caaggcgtat agttaacggt
    17101 gatggctgca agcgcttttt caccactcaa ccaagcgtgg ctgatcgcgc tttgttcggc
    17161 atgaacggtt tgctgcattg ttgcaccaat aaattccata ttggcaccaa aataccaggt
    17221 tccgctcaca ccgcgcgcaa ttgcgccaac attaaaattc gacaatggcg tacgcgcaca
    17281 ggccgccgcc agcggaagta gtgcgaatgc cagcgcgtct tcgtccagtc ccgttgcgct
    17341 cttcagcgat gagacttgct ccccggtcaa caaagcgggg aagtacttgt ctgccagaat
    17401 aggttccagt gcagattgca agttatccgc aagttgggca aaagcggttt gaaaacgtgg
    17461 atgcatggcg ttgcctcata atcatgtaat gggataacag tttacgaacc cgttatggct
    17521 ttatatgtga tctgaatctc attaattatg caacacgtga aacttacatc aaaaatgcgc
    17581 gacgtatcgc aaattttagc ccattactgc cagaataatc gggaaaagga acggtgcgat
    17641 cagcgaagta attatcccgc ataacaccag cgctagcgaa ctaaatgcac cttcctgata
    17701 atccagctcg gcgcagcgcg ccgtaccgag ggcgtgcgag gcagtcccca ttgccagacc
    17761 gcgcgcagct ttggtacgaa tacgcatcgc attaagcaat gtatggccaa ataccgcgcc
    17821 gaggatgccg acgaaaatca cgcaaactgc gctgattgcc ggaataccgc caatactgcc
    17881 gccaaccgcc attgcaatag gcgtggtgac tgatttcggc aggattgacg cggcgatttc
    17941 cggtgaagcg cccatcaaca atgccacgga agtccccgtc accatcgcaa ccacgctgcc
    18001 gataaaacag atggtgatga tcgatttcca gcgcgcgcgg atctggtgta gctgctcata
    18061 taaaggatag gccagcgcga cgaccgccgg ttgcagcaga tcgtttagca cttcgctacc
    18121 tttaaagtaa ctgtcgtaag agatgccggt cagcattaaa aaaggaatga tcaccaccat
    18181 tgctaccagc aacgggttaa gcaggggaaa cttataccgt gccgccagtt tgcgggcggc
    18241 aaaaaagaca atcaacgtca gcggtaatga ccaccagata ttcgccatca ttcttctgat
    18301 cctttctgac ctacgacttt acgttcaccg tgtaccagtt gtgaactcca gctcaccacc
    18361 agaaaaacca ccagcgtact gacagcacag gaaaccacta ccgggccaaa ctgcgcgcgg
    18421 agcaaatcaa aatattgcat gacgcctacg ccaatcggca caaacaatag cgccatatag
    18481 cgaatcagta cgtagcaccc cggattgacc catttcgccg gaagaatttg caaggccagc
    18541 aggacaaaca ggatcagcat cccgatgatg ctgcccggaa tggttaccgg tagcagggaa
    18601 gcaatgaaaa tgcctgcata caggcaggca taaatcagga cgaaagcgcg taaatattgc
    18661 cagataatgt tcagtgtctt actcataatc aacaccccag atgagatgga ttcatcatac
    18721 aattaattcg taaaacgtgc taccgatcac attaagaaat cagcgcttat tttttcgcta
    18781 cccggtatta cgcaatgagc agcgaactca ggcatacatc aagcatagag agggattgca
    18841 gtttgcagca atccctcttt gcagaccttt aatccgctga ccataggcgc taacatagcg
    18901 cctcattttt ttgcgggtga tgatatgcgt gtgttactgg caccgatgga gggagtgctt
    18961 gactctctgg tgcgtgaatt gctgaccgaa gttaacgact acgatctgtg catcaccgag
    19021 tttgtccgcg tggtggatca actgctgccg gtaaaagtct ttcatcgcat ttgccctgag
    19081 ctacaaaacg ccagccggac accatctggt acgctggtgc gcgtgcagtt gttaggtcag
    19141 ttcccacaat ggctggcaga gaacgccgcc cgtgcggtgg agttaggttt ctggggcgtg
    19201 gatctcaatt gcggctgccc gtcgaaaacg gttaacggta gcggcggcgg ggcgacgtta
    19261 ctcaaagatc ctgaactcat ctaccagggg gcaaaagcga tgcgtgaagc tgtaccggcg
    19321 catttgcctg tcagcgtgaa agtgcgtctg ggctgggaca gcggtgagaa gaaatttgaa
    19381 atcgccgatg cggttcaaca ggctggcgct acggagctgg tggtgcatgg gcggacgaaa
    19441 gagcagggtt accgcgcgga gcatattgac tggcaggcga ttggcgagat tcgccagcgg
    19501 ctgaatattc cggtgattgc caacggtgaa atctgggact ggcagagcgc gcaacaatgc
    19561 atggcgatca gcggctgcga cgcagtgatg attggtcgcg gggcgctcaa tattcccaac
    19621 ctgagccggg tggtaaaata taacgaaccg cgaatgccgt ggccggaggt ggttgctttg
    19681 ctgcaaaaat atacccgtct ggaaaagcag ggcgataccg ggttatatca cgttgcgcgg
    19741 attaaacagt ggttgagtta tttgcgtaaa gaatacgatg aagcaacgga attatttcag
    19801 catgttcggg tgttgaataa ctcccctgat attgcaaggg ctattcaggc aattgatatc
    19861 gagaaactct aaaaaccata gtgatgtttt tgacaatgtg ttagctcttt atcaataggc
    19921 cgcacggttg tttggtggcg tcaaaatcgc cgaggcgttc cctgaaggcc ggggcagccc
    19981 acatggatgt gggctgaggg cgcgttttac agggatgtta cctcgcgccc gacccggtag
    20041 ccgtaaggga taagccgag
//
LOCUS       DAZOYF010000068        19393 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZOYF010000068 DAZOYF010000000
VERSION     DAZOYF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.068"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHUOF_002260"
     CDS             <1..122
                     /locus_tag="ACHUOF_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR0611424.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..686
                     /gene="wcaF"
                     /locus_tag="ACHUOF_002261"
     CDS             138..686
                     /gene="wcaF"
                     /locus_tag="ACHUOF_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR0611425.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            712..1833
                     /gene="gmd"
                     /locus_tag="ACHUOF_002262"
     CDS             712..1833
                     /gene="gmd"
                     /locus_tag="ACHUOF_002262"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR0611426.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1836..2804
                     /gene="fcl"
                     /locus_tag="ACHUOF_002263"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1836..2804
                     /gene="fcl"
                     /locus_tag="ACHUOF_002263"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR0611427.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            2804..3283
                     /gene="gmm"
                     /locus_tag="ACHUOF_002264"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             2804..3283
                     /gene="gmm"
                     /locus_tag="ACHUOF_002264"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR0611428.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHUOF_002265"
     CDS             3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHUOF_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR0611429.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHUOF_002266"
     CDS             4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHUOF_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR0611430.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHUOF_002267"
                     /gene_synonym="manB"
     CDS             6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHUOF_002267"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR0611431.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHUOF_002268"
     CDS             7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHUOF_002268"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFR0611432.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHUOF_002269"
     CDS             8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHUOF_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFR0611433.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHUOF_002270"
     CDS             10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHUOF_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFR0611434.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHUOF_002271"
     CDS             11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHUOF_002271"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFR0611435.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHUOF_002272"
     CDS             13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHUOF_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFR0611436.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            14691..15584
                     /gene="galF"
                     /locus_tag="ACHUOF_002273"
     CDS             14691..15584
                     /gene="galF"
                     /locus_tag="ACHUOF_002273"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFR0611437.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHUOF_002274"
     CDS             15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHUOF_002274"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR0611438.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHUOF_002275"
     CDS             17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHUOF_002275"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFR0611439.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            18000..18878
                     /gene="rfbA"
                     /locus_tag="ACHUOF_002276"
     CDS             18000..18878
                     /gene="rfbA"
                     /locus_tag="ACHUOF_002276"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR0611440.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            18883..>19393
                     /gene="rfbC"
                     /locus_tag="ACHUOF_002277"
     CDS             18883..>19393
                     /gene="rfbC"
                     /locus_tag="ACHUOF_002277"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFR0611441.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDIN"
BASE COUNT         4830 a         4690 c         5283 g         4590 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacgc gtgggttggc gatgacgtca atttatatac cctcggtgaa ataaccattg
      421 gcgcacattc ggtgatatcg caaaaaagtt atttatgcac cggtagccac gaccatgcaa
      481 gtcaacattt caccattaac gccacgccta ttgtgattgg cgagaaatgc tggctggcaa
      541 ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg caccgtcgtg ggtgcacgaa
      601 gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg ggggaatccc gcagtggtga
      661 tacgcgaacg cgttgaaact gaataaattc aaaaatacag aggaataaga catgtcaaaa
      721 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
      781 gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat cgtcattcaa caccgagcgc
      841 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
      901 gacctgagtg atacctctaa cctgacgcgc attttgcgtg aagtacagcc ggatgaagtg
      961 tacaacctag gcgcaatgag ccacgttgcg gtctcttttg agtcaccaga atataccgct
     1021 gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
     1081 aagaaaactc gtttctatca ggcttccacc tctgaactgt atggtctggt gcaggaaatt
     1141 ccgcagaaag aaaccacgcc gttctacccg cgatctccgt atgcggtcgc caaactgtac
     1201 gcctactgga tcaccgttaa ctaccgcgaa tcctacggca tgtacgcttg taacggaatt
     1261 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgcaa aatcacccgc
     1321 gcaatcgcca acatcgccca ggggctggag tcgtgcctgt acctcggcaa tatggattcc
     1381 ctgcgtgact ggggccacgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
     1441 gaacagccgg aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagtttgtg
     1501 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag
     1561 aagggcattg tggtttccgt caccgggcat gacgcgccgg gcgttaaacc gggtgatgtg
     1621 attatcgccg ttgatccgcg ttacttccgt ccggcagaag ttgaaacgct gctcggcgac
     1681 ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg
     1741 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
     1801 ggctacgacg tggcgatcgc gctggagtca taagcatgag taaacaacgc atttttatcg
     1861 ctggccattg tgggatggtc ggttctgcca tcaagcggca gctcgaacag cgcggtgatg
     1921 tggagctggt attacgtacc cgcgatgaac tgaacctgct ggacagccgc gccgtgcatg
     1981 atttctttgc cagcgaacgc attgaccagg tctatctggc ggcggcgaaa gtgggcggca
     2041 ttgttgccaa caacacctat ccggcggatt tcatctacca gaacatgatg attgagagca
     2101 acatcattca cgccgcgcat cagaacgacg tgaacaaact gatgtttctc ggatcgtcct
     2161 gcatctaccc gaaactggca aaacagccga tggcagaaag cgagttattg cagggcacgc
     2221 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
     2281 cttacaaccg ccagtatgga cgcgattacc gcactgtcat gccgaccaac ctgtacgggc
     2341 cgcatgacaa cttccacccg agtaactccc atgtgatacc agcattgctg cgtcgcttcc
     2401 acgaggcgac ggcacagaat gcgccggacg tggtggtatg gggcagcggt acaccgatgc
     2461 gtgaattcct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
     2521 atgaagtctg gctggagaat acccaaccga tgctgtcgca cattaacgtc ggcacgggcg
     2581 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc
     2641 gggtggtttt tgatgccagc aaaccggatg gtacgccgcg caaactgctg gatgtgacgc
     2701 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
     2761 accagtggtt ccttgagaat caagaccgct ttcgggggga atgatgtttt tacgtcagga
     2821 agactttgcc actgtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa
     2881 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
     2941 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
     3001 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
     3061 gcatttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
     3121 ttttcgcttc agagtagcgg aagaagagct gttactgccg gatgagcagc atgacgatta
     3181 ccgctggctg acgccggacg cgctgctcgc cagtaacgat gttcacgcta acagccgagc
     3241 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
     3301 attaactact cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg
     3361 ttggcggcac aaggtcatga ggtgcgggtc attaccgcac cgccttacta cccgcaatgg
     3421 caggtgggtg agaactattc cgcctggcgc tacaaacgag aagaaggggc cgccacggtg
     3481 tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc tgaaacgctt gttgcatctc
     3541 ggcagttttg ccgtcagcag tttctttcca ctgatggcgc aacgtcgctg gaagccggat
     3601 cgcattattg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaag
     3661 ctctctggtg cgcgtaccgt gctgcatatt caggattacg aagtggacgc catgctgggg
     3721 ctgggccttg ccggaaaagg caaaggcggc aaagtggcac agctggcaac ggcgttcgaa
     3781 cgtagcggac tgcataacgt cgataacgtt tccacgattt cgcgttcgat gatgaataaa
     3841 gccatcgaaa aaggcgtggc ggcggataac gtcatcttct tccccaactg gtcggaaatt
     3901 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
     3961 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
     4021 aacgttattg aagctgccga tctcctgcgc gatgaaccgc tgatttttgc cattgtcggg
     4081 cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg
     4141 caatttttcc cgctgcaatc gtatgacgct ttacctgcac tgctgaagat gggcgattgc
     4201 catctggtgg tgcaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat
     4261 attctggcgg taggcggtaa cgcggtgatt accgcagagg cccacacaga actggggcag
     4321 ctttgcgaaa cctttccggg tattgcggtt tgcgtagaac cggaatcggt cgaggcgctg
     4381 gtggcgggga ttcgtcaggc gctcctgctg cccaaacata acacggtggc acgtgaatat
     4441 gccgaacgca cgctcgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga
     4501 taattatggc gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgtt
     4561 tatggccgct ttcccgcgta ctttacccca agcagttttt atgcctgaaa ggcgatctca
     4621 ccatgctgca aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga
     4681 tttgcaatga gcagcaccgc tttattgtcg cggaacagct gcgtcaactg aacaaactca
     4741 ccgagaacat tattctcgaa ccggcagggc gtaacactgc acctgccatt gcactggcgg
     4801 cgctggcggc aaaacgtcat agcccggaga gcgacccgtt aatgctggta ttggcggcgg
     4861 atcatgtgat tgccgatgaa gacgcgttcc gtgccgccgt gcgtaatgcc atgccttatg
     4921 ccgaagcggg caagctggtg acctttggca ttgtgccgga tctaccagaa accggttatg
     4981 gctatattcg tcgcggtgaa gtgtctgtgg gtgagcagga tacggtggcc tttgaagtgg
     5041 cgcagtttgt cgaaaaaccg aatctggaaa ccgcccaggc ctatgtagca agcggcgaat
     5101 attactggaa cagcggcatg ttcctgttcc gcgccggacg ctatctcgaa gaactgaaaa
     5161 aatatcgccc ggatatcctc gatgcctgcg aaaaagcgat gagcgccgtc gatccggatc
     5221 tcgattttat tcgtgtggat gaagaagcgt ttctcgcctg cccggaagag tcggtggatt
     5281 acgcggtcat ggaacctacg gcagatgctg ttgtggtgcc gatggatgcg ggctggagcg
     5341 atgttggttc ctggtcttca ttatgggaga tcagcgccca caccgccgag ggcaacgttt
     5401 gccacggcga tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatcaggcc
     5461 tggtcaccac cgtcggggtg aaagatctgg tagtggtgca gaccaaagat gcggtgctga
     5521 ttgccgaccg taacgcggta caggatgtga aaaaagtggt cgagcagatc aaagccgatg
     5581 gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta
     5641 tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gagggtttgt
     5701 cggtacagat gcaccatcac cgcgcggaac actgggtagt ggtcgcggga acggcaaaag
     5761 tcaccattga tggtgatatc aaactgcttg gtgaaaacga gtccatttat attccgctgg
     5821 gggcgacgca ctgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct
     5881 ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt
     5941 aaacgtcgca tcaggcgctg tttgtcggat gcggcgtgaa cgccttatcc gacctacggt
     6001 tcggttttgt aggcctgata agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg
     6061 cggtgtaaat aacgacaaaa ataaaattgg ccgtttcggt cagggccaac tattgcctga
     6121 aaaagggtaa cgacatgaaa aaattaacct gctttaaagc ctatgatatt cgcgggaaat
     6181 taggcgaaga actgaatgaa gatatcgcct ggcgcattgg tcgcgcctat ggcgaatttc
     6241 tcaaaccgaa aaccattgtg ttaggcggtg atgtccgtct caccagcgaa accttaaaac
     6301 tggcgctggc aaaaggttta caggatgcgg gcgtcgatgt gctggatatc ggtatgtccg
     6361 gcaccgaaga gatctatttc gccacgttcc atctcggcgt ggatggcggc attgaagtta
     6421 ctgccagcca taatccgatg gattacaacg gcatgaagct ggtgcgcgag ggggctcgca
     6481 cgatcagcgg tgataccgga ctgcgcgacg tccagcgtct ggctgaagcc aacgactttc
     6541 ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat caacctgcgt gacgcttacg
     6601 ttgatcacct gttcggttat atcaatgtca aaaatctcac gccgctcaag ctggtgatca
     6661 actccgggaa tggcgcagcg ggtccggtgg tggacgccat tgaagcccgc tttaaagccc
     6721 tcggcgcgcc cgtggaatta atcaaagtac acaacacacc ggacggcaat ttccccaacg
     6781 gtattcctaa cccgctgctg ccggaatgtc gcgacgacac ccgcaatgcg gtcatcaaac
     6841 acggcgcgga tatgggcatt gcctttgatg gcgattttga ccgctgtttt ctgtttgacg
     6901 aaaaagggca gtttatcgag ggctactaca ttgtcggcct gctggcagaa gcgttcctcg
     6961 aaaaaaatcc cggcgcgaag atcatccatg atccgcgtct ctcttggaac actattgatg
     7021 tggtgactgc tgcaggcggc acgccggtaa tgtcgaaaac cggacacgcc tttattaaag
     7081 aacggatgcg caaggaagac gccatctacg gtggcgaaat gagcgcccac cattacttcc
     7141 gtgatttcgc ttactgcgac agcggcatga tcccgtggct gctggtcgcc gaactggtgt
     7201 gcctgaaagg aaaaacgctg ggcgaactgg tgcgcgaccg gatggcggcg tttccggcaa
     7261 gcggtgagat caacagcaaa ctggcgcaac ccgttgaggc gattaaccgc gtggaacagc
     7321 attttagccg tgaggcgctg gcggtggatc gcagcgatgg catcagcatg acctttgccg
     7381 actggcgctt taacctgcgc acctccaata ccgaaccggt ggtgcgcctg aatgtggaat
     7441 cgcgcggtga tgtgccgctg atggaagcgc gaacgcgaac tctgctgacg ttgctgaacg
     7501 agtaatgtcg gatcttccct taccccactg cgggtaaggg gctaataaca ggaacaacga
     7561 tgacaaatct aaaaaagcgc gagcgagcga aaaccaatgc atcgttaatc tctatggtgc
     7621 aacgcttttc agatatcacc atcatgtttg ccgggctatg gctggtttgc gaagtcagcg
     7681 gactgtcatt cctctacatg cacctgttgg tggcgctgat tacgctggtg gtgttccaga
     7741 tgctgggcgg catcaccgat ttttatcgct catggcgcgg tgttcgggca gcgacagaat
     7801 ttgccctgtt gctacaaaac tggaccttaa gcgtgatttt cagcgccgga ctggtggcgt
     7861 tcaacaatga tttcgacacg caactgaaaa tctggctggc gtggtatggg ctgaccagca
     7921 tcggactggt ggtttgccgt tcatgtattc gcattggggc gggctggctg cgtaatcatg
     7981 gctacaacaa gcggatggtc gccgtagcgg gggatttagc cgccggacaa atgctgatgg
     8041 agagtttccg taatcagccg tggttagggt ttgaagtggt gggcgtttac cacgacccga
     8101 aaccgggcgg cgtttctaac gactgggcgg gtaacctgca acagctggtc gaggatgcaa
     8161 aagcgggcaa gattcataac gtctatatcg cgatgcaaat gtgcgacggc gcacgagtga
     8221 aaaaactggt ccatcaactg gcggatacca cctgttcggt gctgctgatc cccgacgtct
     8281 ttaccttcaa cattctccat tcacgtctcg aagagatgaa cggcgttccg gtggtgccgc
     8341 tgtatgacac gccgctttcg ggggttaacc gcctgctaaa acgtgcggaa gacattgtgc
     8401 tggcgacgct tattctgctg ctgatctccc cggtgctgtg ctgtattgcg ctggcggtga
     8461 aactcagttc accagggccg gttattttcc gccagactcg ctatggtatg gatggcaagc
     8521 cgatcaaagt gtggaagttc cgttccatga aagtgatgga gaacgacaaa gtggtgaccc
     8581 aggcgacgca gaacgatccg cgcgtcacta aagtggggaa ctttctgcgc cgcacctcgc
     8641 tggacgaatt gccgcagttt atcaatgtgc tgaccggggg gatgtcgatt gtcggtccac
     8701 gtccgcacgc agtagcgcat aacgaacagt atcgccagct cattgaaggc tacatgctgc
     8761 gccataaagt gaaaccgggc attaccggct gggcgcagat taatggctgg cgcggcgaaa
     8821 ccgacacgct ggagaaaatg gaaaaacgcg tcgagttcga ccttgagtac atccgcgaat
     8881 ggagcgtctg gttcgatatc aaaatcgttt tcctgacggt attcaagggc ttcgttaaca
     8941 aagcggcata ttgatatgag cttacgtgaa aaaaccatca gcggcgcgaa gtggtcggct
     9001 attgccacgg tgatcatcat cggcctcggg ctggtgcaga tgaccgtgct ggcgcggatt
     9061 atcgacaacc accagttcgg cctgctgact gtgtcgctgg tgattatcgc gctggcagat
     9121 acgctttctg acttcggtat cgctaactcg attattcagc gaaaagaaat cagtcacctt
     9181 gaactcacca cgctgtactg gctgaacgtc gggctggggc ttgtggtatg tgtggcggtg
     9241 tttttgttga gtgatgtcat cggcgacgtg ctgaataacc cggacctggc accgttgatt
     9301 aaaacattgt cgctggcgtt tgtggtgatc ccccacgggc aacagttccg cgcgttgatg
     9361 cagaaggagc tggagttcaa caaaatcggc atgatcgaaa ccagcgcggt gctggcgggc
     9421 ttcactttta cggtagttag cgcccatttc tggccgctgg cgatgaccgc gatcctcggt
     9481 tatctggtca atagtgcggt gagaacactg ctgtttggct actttggacg caaaatttat
     9541 tgccccggtc tgcacttctc gctggcgtct gtggcaccga acttacgttt tggtgcctgg
     9601 ctgacggcgg acagcatcat caactatctc aacaccaacc tttcaacgct ggtgctggcg
     9661 cgtattcttg gcgcgggcgt ggcaggggga tacaacctgg cgtacaacgt ggccgtcgta
     9721 ccgccgatga agctgaaccc aatcatcacc cgcgtgttgt ttccggcgtt tgccaaaatt
     9781 caggacgaca ccgaaaagct gcgtgttaac ttctacaagc tgctgtcgat agtggggatt
     9841 atcaactttc cggcgctgct ggggctgatg gtggtgtcga ataacttcgt accgctggta
     9901 ttcggcgaaa agtggaacag cattattccg gtgctgcaat tgctgtgtat tgtgggtctg
     9961 ctgcgctccg tgggtaaccc gattggttcg ctgctgatgg cgaaagcgcg ggtcgatatc
    10021 agctttaaat tcaacgtatt caaaacattt ctgtttattc cggcgattgt tattggtggg
    10081 cagatggcgg gcgcaatcgg cgtcacgctc ggtttcctgc tggtgcagat tatcaacacc
    10141 attctgagtt acttcgtgat gattaaaccg gttcttggtt ccagttatcg ccagtacatc
    10201 ctgagtttgt ggctgccgtt ttatctctcg ttgccgacgc tggtggtcag ttatgcgctg
    10261 ggcattgtgc tgaaagggca actggcgctg gggatgctgc tggcggtgca aatagccgcg
    10321 ggcgtgctgg cgtttgtggt gatgattgtg ctgtcacgcc atccgctggt ggtggaagtg
    10381 aagcgtcagt tttgtcgcag cgaaaaaatg aaaatgcttt tacgggcggg gtgaattgct
    10441 gttttcggtt ccgacaacgg ttgccggatg cggcgtaaaa cgccttatcc gtcctacggt
    10501 tctgtgctcg ggacctgtag gcctgataag acgcgccagc gtcgcatcag gcaaaaaacg
    10561 gcaacgaaca caacaaacac agcctcacct ggcatcaaat ttatgaggaa aaaatgaaat
    10621 tacttattct gggcaaccac acttgcggca atcgtggcga cagcgccatc ttgcgcggct
    10681 tacttgatgc catcaacatc ctcaatccac ataccgaagt ggacgtgatg agccgctatc
    10741 cggtcagttc ttcctggctg ctcaaccgcc cggtaatggg cgatccgctg ttcctgcaga
    10801 tgacacaaca caatagcgcg gcgggcgttg tcgggcgcat taaaaaagtc ctccgtcgcc
    10861 gctaccagca tcaggtattg ctctcacgcg tcaccgacac tggcaagctg cgcaatatcg
    10921 ccatcgccca gggattcacc gacttcgtgc gcctgctgtc aggttacgac gccattattc
    10981 aggtcggcgg atcgtttttt gtcgatctct acggcgtgcc gcagtttgaa catgcgcttt
    11041 gcacatttat ggcgaaaaag ccgctgttta tgattggtca cagcgtcggc cccttccagg
    11101 atgagcaatt taaccaactg gcgaactatg tctttggtca ctgcgacgcg ctgatcctgc
    11161 gcgaatcggt cagccttgat ttgatgaaac gcagaaatat caccaccgca aaagtggaac
    11221 atggcgtcga taccgcgtgg ctggtcgatc accacaccga agacttcacc gccagctatg
    11281 ccgttcaaca ctggctggac gttgccgcac aacagaaaac agtggcaatt accctgcgcg
    11341 aactggcacc gttcgacaaa cgtctcggca ccactcaaca agcgtatgaa aaagcctttg
    11401 ccggggtggt caatcgcatt ctcgatgaag gctatcaggt gattgcgctc tccacctgta
    11461 cgggtatcga cagctataac aaagacgacc gcatggtggc gctcaatctg cgccagcaca
    11521 tcagcgatcc tgcccgttac cacgtagtga tggatgaact caacgatctg gaaatgggca
    11581 aaattctcgg agcctgtgaa ctcaccgtcg gtacgcgcct gcactccgcc attatctcaa
    11641 tgaactttgc tactccggcg attgccatca actatgaaca taaatccgcc gggattatgc
    11701 agcagctggg actgccagag atggcgattg atattcgtca tttactggac ggcagcctgc
    11761 aagcgatggt tgcggatacc ttaggccagc ttccggtgct gaacgctcga tttaacgaag
    11821 ccgtcagtcg cgaacgtcag acgggaatgc agatggtgca gtctgtgctt gagcgcatcg
    11881 gggaggtgaa atgaaggtcg gcttcttctt attgaaattc ccgctgtcgt cggaaacctt
    11941 cgtcctcaat caaattaccg cgtttattga tatgggcttt gaagtggaga ttgtcgcttt
    12001 gcaaaaaggc gatacagaga acacccacgc ggcatggacg aaatacaacc ttgtcgccag
    12061 aacccgctgg ttacaggacg aacctgcggg caaagtagcg aaactgcgcc accgcgccag
    12121 ccagacctta cgtggcattc atcgtaaaaa tacctggcat gcgctcaacc tcaaacgcta
    12181 tggtgccgag tcgcggaacc tgattttgtc tgccatttgt ggtcaggtcg caacaccgtt
    12241 tcgcgccgat gtgttcatcg ctcattttgg cccggcgggg gtaaccgcag caaaactacg
    12301 cgaactgggt atcattcgcg gcaaaattgc caccatcttc cacggtattg atatctccag
    12361 tcgggaagtg ctcaaccact acactcccga atatcaacaa ctgtttcgcc gtggcgacct
    12421 gatgttaccg ataagcgatc tgtgggccgg aaggctgcaa aaaatgggct gccccagaga
    12481 aaaaatcgcc gtatcgcgta tgggcgtgga catgacgcgc tttagcccgc gtccggtgaa
    12541 agcgcccgca acgccgctgg aaatcatatc tgtcgcacgc ttaaccgaga aaaaaggcct
    12601 gcatgtggcg attgaagcct gccgtcagtt gaaagagcag ggcgtggcat ttcgctatcg
    12661 catcctcggc attggcctgt gggaacgacg cctgcgcacc atcatcgaac aatatcaact
    12721 ggaagatgtg gtagagatgc cgggctttaa accgagccat gaagtgaagg agatgctcga
    12781 cgacgcggat gtcttcctgt tgccatcggt aacgggcgcg gatggcgata tggaaggcat
    12841 tccggtggcg ctaatggaag cgatggcggt cggtattccg gtggtttcta ctctgcatag
    12901 cggaattcct gaactggtgg aggccgataa atccggctgg ctgctgcctg agaacgatgc
    12961 tcgcgcactg gcacaacgtc tggcggcgtt tagccaactg gataccgacg aactggctcc
    13021 ggtcgtcaaa cgtgcgtgcg aaaaagtcga acacgatttt aaccagcagg tgattaatcg
    13081 agaactcgcc agcttgctgc aggctttata gaggagaacg tatgccattt aaaacactct
    13141 cccgacgcac cttcctgacg gcaagctcgg cgcttgcctt cctccatacc cctttcgctc
    13201 gcgcacttcc cgcccgacaa agcgttaaca ttaacgacta caacccacat gactggatcg
    13261 cctcatttaa acaagccttc agcgaagggc aaacggtcgt cgtgcctgcc ggattcgttt
    13321 gtgacaatat caacaccggc atcttcatcc ctcccggcaa aacgttacac atccttggaa
    13381 gcctgcgcgg caacggcaga gggcgatttg tcttacagga cggcagccag gtgacagggg
    13441 gagagggcgg cggtatgcat aacattaccc tggatgtgcg tggttctgac tgcaccatca
    13501 aagggctggc gatgagcggc tttggcccgg taatgcagat ttatattggc ggcaaaaaca
    13561 aacgggtcat gcgcaacctg accatcgata acctcactgt cagccacgct aattacgcca
    13621 tcttacgcca gggatttcat aaccagatta tcggtgccaa cattaccaac tgtaagttca
    13681 gcgacttaca gggcgacgcc attgaatgga acgtggcaat taacgacagt gatattttga
    13741 tatctgacca tgtcatcgag cgtatcaact gtaccaacgg caaaatcaac tggggcatcg
    13801 gcataggcct tgcgggaagc acttatgata acaactaccc ggaagaccag gcagtgaaaa
    13861 actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat gttgaaaatg
    13921 gtaaacattt tgttattcgt aatatcaatg cccgcaatat cacgccggat ttcagtaaga
    13981 aagcgggcat tgataacgcg acagtcgcta tttacggttg tgacaatttc gtgattgata
    14041 atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt aaaggcaaat
    14101 atctctcgat accgcaaaat ttccgagtga ataatattca actggataat acccatcttg
    14161 cttataaatt gcgcggcatc caaatctccg ccgggaatgc cgtctccttt gtgtcgctaa
    14221 ctaacattga gatgaagcgt gcctcgctgg agttacacaa caagccgcaa catcttttta
    14281 tgcgaaatat caatgtgatg caggaatcct cagttggacc agcattgagc atgaacttcg
    14341 acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaaa agaaacactg ctgtctcttg
    14401 caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac aggattaatc
    14461 accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg gagtagattt
    14521 gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca atccacaacg
    14581 aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat gctggcgaaa
    14641 aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata atgacgaatt
    14701 taaaagcagt tattcctgta gcgggtctcg ggatgcatat gttgcctgcc actaaggcga
    14761 tacccaaaga gatgctacca atcgtcgaca agccaatgat tcagtacatt gttgacgaga
    14821 ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg
    14881 aaaaccactt cgacacctct tatgagttag aatcactcct tgagcagcgc gtgaagcgtc
    14941 aactgctggc ggaagtacag tccatctgtc cgccgggcgt gatcattatg aacgtgcgtc
    15001 agggcgaacc tttaggttta ggccactcca ttttgtgtgc gcgacctgcc attggtgaca
    15061 acccatttgt cgtggtactg ccagacgttg tgatcgacga tgccagcgcc gacccgctac
    15121 gttacaacct tgctgccatg attgcacgtt tcaacgaaac gggccgcagc caggtgctgg
    15181 caaaacgtat gccgggtgac ctctctgaat actccgtcat ccagactaaa gagccgctgg
    15241 accgtgaggg taaagtcagc cgcattgttg aatttatcga aaaaccggat cagccgcaga
    15301 cgctggactc agacatcatg gccgtaggtc gctatgtgct ttctgccgat atttggccgg
    15361 aactggaacg tactcagcct ggtgcatggg gacgtattca gctgactgat gctattgccg
    15421 agctggcgaa aaaacaatcc gttgatgcaa tgctgatgac cggcgacagt tacgactgcg
    15481 gcaaaaaaat gggctatatg caggcgtttg tgaagtatgg cctacgcaac ctgaaagaag
    15541 gggcgaagtt ccgtaaaggt attgagaagc tgttaagcga ataatgaaaa tctgaccgga
    15601 tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcataa gttttgctca
    15661 ttgaattttc ctgccgttgc cgtttttata aagcctataa aaacaagaag ttacatagct
    15721 catattattc aaatttttcc aggattttcc ttgtttctac ggctgtttgg taagacaatt
    15781 aacgtttgaa tttttcggat ttggcgcgag tggctaacgc tcgccacatc gttgagatgc
    15841 atgcagtgca ctggtagctg ttaagccagg ggcggtagcg tgtattaata cctctattaa
    15901 tcaaatctgg agcagtctat ttcacagcat gctctatagc tatatggaat aaaaaagtga
    15961 agatacttgt tactggtggc gcagggttta ttggttctgc tgttgttcgt cacataataa
    16021 ataatacgca agatagtgtt gttaatgtcg ataaattaac atacgccggg aacctggaat
    16081 cactcgctga tgtttctgat tctgaacgtt atgcttttga gcatgcagat atctgcgatg
    16141 ctgtagccat gtcgcgtatt ttcgcacagc accagccaga cgcggtgatg cacctggcgg
    16201 cagagagcca cgttgaccgt tcaataacgg gccctgcggc atttattgaa accaatattg
    16261 tggggactta tgttctttta gaagcggcgc gcaattactg gtctgctctg aatgatgaaa
    16321 agaaaaaaag tttccgcttt catcatattt ctactgatga agtttatggt gacttacccc
    16381 atccggatga agcaaataat aacgaagcgt taccgctatt tacggaaacg acagcttacg
    16441 cgccaagtag cccgtattct gcgtctaaag cttccagcga tcatttggta cgtgcctgga
    16501 agcggactta tggtttacca accattgtga ctaattgctc gaacaattat ggtccttatc
    16561 acttcccgga aaaacttatt ccattggtta ttctgaatgc tctggaaggt aaggcattac
    16621 ctatttatgg taaaggggat caaattcgcg actggttgta tgtagaggat catgctcgtg
    16681 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacata
    16741 acgaaaagaa aaacatcgac gtagtgctca ctatttgtga tttgttggat gagattgtac
    16801 cgaaagagaa atcttatcgt gagcaaatta cttatgttgc cgatcgcccg gggcatgacc
    16861 gccgttacgc cattgatgcc gataaaatta gccgcgaatt gggctggaaa ccgcaagaaa
    16921 cgtttgagag cgggattcgt aaaacggtgg aatggtatct ggctaataca aattgggttg
    16981 agaatgtgaa aagcggtact tatcagtcgt ggattgaaca aaactatgag ggccgtcagt
    17041 aatgaatatc ctgcttttcg gcaaaacagg gcaggtgggt tgggaactgc agcgtgctct
    17101 ggctcctctg ggtaacttga ttgcgctcga tgttcactcc actgattatt gtggagattt
    17161 cagcaacccc gaaggtgtgg ctgaaaccgt caaaaaaatt cgccccgatg ttattgttaa
    17221 tgctgctgct cacactgcag tagacaaagc ggaatcagaa ccgaatttcg cgcaattact
    17281 taacgcgaca tgcgtcgaag cgattgcaaa agcagctaat gaagtcggag cctgggttat
    17341 acactactcc actgattatg ttttcccagg caacggtgac acgccatggc tggaaacgga
    17401 tgcaacagca ccgctaaatg tctacggtga aaccaagcta gctggggaaa aagcgttaca
    17461 agaacattgc gcaaagcatc ttattttccg taccagctgg gtatacgctg gtaaaggaaa
    17521 taactttgcc aaaacgatgt tgcgcctggc aaaagaacgc gaagagttgg ctgtaataaa
    17581 cgatcaattc ggcgcaccaa cgggggctga attactggct gattgcaccg ctcatgccat
    17641 tcgcgtggcc gcaaataaac cagaagttgc tggtttgtac catctggttg ctggtggtac
    17701 aacaacctgg cacgattatg ccgctctggt atttgaagaa gcgcgcagag cggggatcaa
    17761 ccttgctctt aacaaactta atgccgtgcc gacaacggcc tatcccacac cagcacgtcg
    17821 tccccataat tctcgcctca ataccgaaaa atttcagcag aactttgcgc ttgtcttgcc
    17881 tgactggcag gtgggcgtga aacgtatgct caacgaatta tttacgacca cggcaattta
    17941 acaaattatt gcatctcgct catgatgcta gagcgagatg aattaaaagg aatggtgaaa
    18001 tgaaaacgcg taaaggtatt attctggctg gtggttccgg cactcgtctt tatcctgtga
    18061 caatggctgt gagcaaacaa ctgctaccga tttatgataa accgatgatt tattatccgc
    18121 tttcaacgct tatgttggcg ggtattcgag atattctgat tatcagtacg ccgcaggata
    18181 caccacgttt tcaacaattg cttggtgatg gaagccagtg ggggcttaat ctgcaatata
    18241 aagtacaacc gagtccggat ggtctggcgc aagcatttat cattggtgaa gaatttatag
    18301 gtggtgatga ttgtgcgctg gtacttggtg ataatatctt ctatggtcac gacctgccga
    18361 aattaatgga taccgctgtt aacagagaga gtggcgcaac ggtttttgcc tatcacgtaa
    18421 atgatcctga gcgctacggt gtggtggaat ttgatgataa cggtacggca attagtctgg
    18481 aagaaaaacc gcaggaacca aaaagtaact atgcggttac tgggctttat ttctatgaca
    18541 atgacgttgt agaaatggcg aaaaacctta aaccatctgc acgtggcgaa ctggaaatta
    18601 ccgatattaa ccgtatttat atggaccaag gacgtttgtc tgtcgctatg atggggcgtg
    18661 gttatgcatg gctggacacg gggacacatc aaagtcttat tgaagcaagc aacttcattg
    18721 caacaattga agagcgtcag gggctgaaag tttcctgccc ggaagaaatt gctcaccgta
    18781 aaggctttat tgatgctgag caagtgaagg tattagccga gccgctgaag aaaaatgctt
    18841 atggtcagta tctgctgaaa atgattaaag gttattaata aaatgaacgt aattaaaact
    18901 gaaattcctg atgtgctaat ttttgaacca aaagtatttg gtgatgagcg gggctttttt
    18961 atggaaagct tcaatcagaa agttttcgaa gaggctgtag gacgaaaggt tgagtttgtt
    19021 caggataacc attctaaatc aactaagggt gtgttacgcg gactgcacta tcagctggaa
    19081 ccttatgctc aaggaaaact ggtacgctgt gttgtcggtg aagtttttga cgtagctgtt
    19141 gatattcgga aatcatcacc aacatttggc aagtgggttg gtgtaaattt gtcggcggag
    19201 aataagcgtc aattatggat accggaagga ttcgctcatg ggttttgcgt tcttagtgat
    19261 gaagcagaat ttgtctacaa aacaaataat ttctattcaa aaatgcaaga gcgtgggatt
    19321 ttatggagcg acaaaagtat aaatatagaa tggccggttc aaaatccatt gctttctgat
    19381 aaagatatta atg
//
LOCUS       DAZOYF010000069        19283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZOYF010000069 DAZOYF010000000
VERSION     DAZOYF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.069"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            23..1186
                     /locus_tag="ACHUOF_002278"
     CDS             23..1186
                     /locus_tag="ACHUOF_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFR0611442.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            1187..2413
                     /locus_tag="ACHUOF_002279"
     CDS             1187..2413
                     /locus_tag="ACHUOF_002279"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR0611443.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            2410..3288
                     /locus_tag="ACHUOF_002280"
     CDS             2410..3288
                     /locus_tag="ACHUOF_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR0611444.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            3299..3652
                     /locus_tag="ACHUOF_002281"
     CDS             3299..3652
                     /locus_tag="ACHUOF_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR0611445.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            3664..4968
                     /locus_tag="ACHUOF_002282"
     CDS             3664..4968
                     /locus_tag="ACHUOF_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR0611446.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            4980..6065
                     /locus_tag="ACHUOF_002283"
     CDS             4980..6065
                     /locus_tag="ACHUOF_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR0611447.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6225..6956)
                     /gene="frlR"
                     /locus_tag="ACHUOF_002284"
                     /gene_synonym="yhfR"
     CDS             complement(6225..6956)
                     /gene="frlR"
                     /locus_tag="ACHUOF_002284"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR0611448.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(7056..7841)
                     /gene="frlD"
                     /locus_tag="ACHUOF_002285"
     CDS             complement(7056..7841)
                     /gene="frlD"
                     /locus_tag="ACHUOF_002285"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR0611449.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(7838..8668)
                     /gene="frlC"
                     /locus_tag="ACHUOF_002286"
     CDS             complement(7838..8668)
                     /gene="frlC"
                     /locus_tag="ACHUOF_002286"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFR0611450.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(8718..9740)
                     /gene="frlB"
                     /locus_tag="ACHUOF_002287"
     CDS             complement(8718..9740)
                     /gene="frlB"
                     /locus_tag="ACHUOF_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR0611451.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(9761..11098)
                     /gene="frlA"
                     /locus_tag="ACHUOF_002288"
                     /pseudo
     CDS             complement(9761..11098)
                     /gene="frlA"
                     /locus_tag="ACHUOF_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            complement(11393..11560)
                     /locus_tag="ACHUOF_002289"
     CDS             complement(11393..11560)
                     /locus_tag="ACHUOF_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR0611452.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(11807..13180)
                     /gene="cysG"
                     /locus_tag="ACHUOF_002290"
     CDS             complement(11807..13180)
                     /gene="cysG"
                     /locus_tag="ACHUOF_002290"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR0611453.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(13199..14005)
                     /gene="nirC"
                     /locus_tag="ACHUOF_002291"
     CDS             complement(13199..14005)
                     /gene="nirC"
                     /locus_tag="ACHUOF_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR0611454.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            complement(14131..14457)
                     /gene="nirD"
                     /locus_tag="ACHUOF_002292"
     CDS             complement(14131..14457)
                     /gene="nirD"
                     /locus_tag="ACHUOF_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR0611455.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(14454..16997)
                     /gene="nirB"
                     /locus_tag="ACHUOF_002293"
     CDS             complement(14454..16997)
                     /gene="nirB"
                     /locus_tag="ACHUOF_002293"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR0611456.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(17259..18439)
                     /gene="tsgA"
                     /locus_tag="ACHUOF_002294"
                     /pseudo
     CDS             complement(17259..18439)
                     /gene="tsgA"
                     /locus_tag="ACHUOF_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
     gene            18710..>19283
                     /gene="ppiA"
                     /locus_tag="ACHUOF_002295"
                     /gene_synonym="rotA"
     CDS             18710..>19283
                     /gene="ppiA"
                     /locus_tag="ACHUOF_002295"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR0611457.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKV"
BASE COUNT         4558 a         4972 c         5196 g         4557 t
ORIGIN      
        1 ggaacagaaa cgaggcaaca agatgtttgt agaagcattg aaacgccaga acccggcgct
       61 gatttccgcc gcactaagtt tgtggcagca gggcaaaatc gccccggaca gctgggtgat
      121 cgacgtggat cagatactgg aaaacggtaa gcggctcatt gagacggcgc ggctttacgg
      181 cattgaactg tatctgatga ccaagcaatt tggtcgtaat ccgtggctgg cggaaaaatt
      241 gctggcatta ggctacagcg gcattgtggc ggtggattac aaagaggcgc gagtcatgcg
      301 ccgcgctggt ttgccagtgg cgcatcaggg gcatctggta caaatccctt gtcatcaggt
      361 tgctgacgcc gttgaacagg gcaccgacat catcaccgtg tttactctcg acaaagcgcg
      421 ggaagtttct gcggcagcgg ttaaggccgg acgggtccag cctgtgctgc tcaaagttta
      481 tagcgacgat gattttcttt atccggggca ggagagcggt tttgtacagc attcgctgca
      541 cgaggtgatt gctgaaatta agaaactgcc ggggctgcat ttagccggac taacccattt
      601 cccttgcctg ctttgggatg aggctgctgg gaaagttttg ccaacaccga atcttcacac
      661 gctggtacaa gcacgggatc aactggcgaa atctggtatt gcgattgagc aactgaacgt
      721 accttcagcg accagctgca cttcgctgcc attgctggcg gaatacggtg cgactcatgc
      781 cgaacccggt catgcgctga ccggtactat tccggcaaac cagcagggcg atcagcctga
      841 acgtatcgcg atgctctggt taagtgaaat ctcccatcat ttccgtggcg acagctactg
      901 ctacggcggc ggttactacc gtcgtggtca tgcgcaacat gcgctggtgt ttacgccaga
      961 aaatcaaaag attactgaaa ccaatctcaa aactgtggat gacagcagta tcgactacac
     1021 cctgccgctg gcaggcgagt atccggtgag cagtgcagtg gtgctctgtt ttcgcacgca
     1081 gatttttgtc acccgtagcg atgtggtgct ggtgtccggt attcagcgcg gcgaaccaga
     1141 aatagttggt cgttatgaca gcctcggcaa ccctctggag gcataaatgg cgcgatttgt
     1201 ggtgttagtg attgatagct ttggcgtagg ggcaatgaaa gatgtcacgc tggtgcgccc
     1261 gcaagatgcc ggggcgaata cctgtggtca catcctgagc cagttgccgc atttgcagct
     1321 accaacgctg gagaagctgg ggctaatcaa cacattgggt tatgcgccag gcgatatgca
     1381 gccgtcagat tccgcaacct ggggcgtggc agagctgcaa catgaaggtg gcgatacctt
     1441 tatggggcat caggaaattt taggcacgcg cccgttaccg ccgctgcgga tgccttttcg
     1501 cgatgtgatt gaccgtgttg agcaggcatt agtttccgct ggctggcagg tggagcgccg
     1561 tggcgatgat ctgcaatttc tgtcggtcaa tcaggcggtt gcgattggcg ataatctcga
     1621 ggcggattta ggccaagtct ataacattac cgccaatctc tctgtgatct cttttgacga
     1681 cgcaatcaaa attggtcgta tcgtgcgtga ccaggtacag gtcggtcggg tcattacatt
     1741 tggcggcctg ttaaccaaca gtcagcgtat tctcgatgcc gcagaaagca aagaagggcg
     1801 ctttattggt attaatgcgc cgcgttctgg cgcttatgac aacggcttcc aggtcgtgca
     1861 tatgggctac ggtgttgatg aaaaagtgca ggtgccgcaa aaactgtatg aagcgggcgt
     1921 gccaaccgtg ctggtgggta aggtggcaga tatcgtcagc aatccttatg gtgtgagctg
     1981 gcaaaacctg gtggatagcc agcggattat ggatatcacc ctcgacgaat ttaacaccca
     2041 tccgacggcg tttatttgca tcaatatcca ggaaaccgac ctcgctggtc atgcagaaga
     2101 cgtcgcacgt tatgccgaac gtttgcaggt cgttgaccgt aaccttgccc ggcttgttga
     2161 ggcgatgcag ccagatgatt gcctggtcgt gatggcggat cacggtaacg atccgaccat
     2221 tggtcacagc caccataccc gtgaagtggt gccagtgctg gtttatcagc aagggctggt
     2281 cgccacgcag ctcggtgtgc gcaccacgct ttctgatgtg ggagctaccg tgtgtgaatt
     2341 tttccgtgcg ccaccgccac aaaatggtcg ctcttttctt tcctccctcc ggtttgcagg
     2401 agacacccta tgagttttga tccgacgggt tacaccctgg cccatgagca tctgcatatt
     2461 gatctctccg gctttaaaaa caacgtggac tgccgccttg atcagtatgc gttcatttgc
     2521 caggagatga acgacctgat gacccggggt gtacgtaatg tgattgagat gaccaaccgt
     2581 tacatggggc gcaatgcgca atttatgctc gatgtaatgc gcgaaaccgg gatcaacgtg
     2641 gtggcctgta ctggttatta ccaggatgcg tttttcccgg aacatgtggt gacccgcagc
     2701 gtgcaggaac tggcgcagga gatggtcgat gaaattgaac agggtatcga tggcacggag
     2761 ctgaaagccg ggatcatcgc ggagatcggc tccagcgaag gaaagattac gccgctggaa
     2821 gagaaggtat ttattgccgc tgcgctggcg cataaccaga ccggacgccc gatctccacg
     2881 catacgtcgt tcagcaccat ggggctggaa caactggctc tgttgcaagc ccacggagtt
     2941 gatctttcgc gcgtcaccgt tggtcactgc gatctgaaag acaacctcga caacattttg
     3001 aagatgatcg atctcggcgc gtacgtgcag ttcgacacca ttggtaagaa cagttactac
     3061 ccggacgaaa agcgcattgc gatgctccac gcgctacgtg accgtgggtt gctgaaccgc
     3121 gtcatgctgt cgatggatat tacgcgccgc tcccatttaa aagccaatgg tggttatggc
     3181 tatgactatt tattgaccac ctttattccg caattgcgcc agtcaggatt cagtcaggcc
     3241 gatgtggatg tgatgttacg tgaaaatccc tctcaatttt tccaataagg acagactcat
     3301 gaaaaagatt ggcgttgcag gcttacagcg tgagcagatt aaaaaaacca ttgaagcgac
     3361 ggctcctggc tgttttgaag ttttcattca caacgacatg gaagcggcaa tgaaggtgaa
     3421 atccgggcaa ctagattatt acattggcgc gtgtaatacc ggtgcgggcg cggcattgtc
     3481 gattgccatc gcggtgattg gctataacaa aagttgcacc attgccaaac caggcattaa
     3541 agcgaaagac gagcatatcg ccaaaatgat cgctgaaggg aaagtggcgt ttggcctttc
     3601 cgttgagcac gtcgaacacg cgattccgat gttgattaac catctgaaat aaaaggcacg
     3661 actatggatc tgtatattca gattatcgtg gtggcgtgcc tgacgggtat gacatcgctt
     3721 ctggagcatc gctcggcggc tgtttttcat gacggcatcc acccgatcct gccgcaactg
     3781 attgaaggct atatgaaccg tcgcgaggcg gggagtatcg cttttggtct gagcattggt
     3841 tttgtggcct cggtggggat ctcttttacc ctgaaaaccg ggctgctcaa cgcatggtta
     3901 ctctttcttc ctaccgatat cctcggcgta ctggcgataa acagcctgat ggcgtttggt
     3961 cttggcgcta tctggggcgt gttgatcctt acttgcctgt tgccagttaa ccagctgctg
     4021 accgcgctgc cggtggatgt attaggtagc ctcggggaat taagctcgcc ggtggtttct
     4081 gcttttgcac tgttcccgct ggtggcgatt ttctaccagt ttggctggaa gcaaagtctg
     4141 gtcgccgccg tggttgttct gatgacccgt gtggtagtcg tgcgctattt cccacatctt
     4201 aaccctgaat ccatcgaaat ctttattggc atggtgatgc tgctggggat cgcgataact
     4261 cacgacctgc gtcatcgtga tgaaaatgac atcgatgcca gcgggctttc ggtgtttgaa
     4321 gagcgcacgt cgcggattat caaaaactta ccttatatcg ccatcgtggg agcattgatt
     4381 gccgccgttg ccagtatgaa gatttttgct ggtagcgaag tttcgatctt cacactggag
     4441 aaagcgtatt ccgcaggcgt gacgccggaa caatcgcaaa cgctgattaa tcaggcggct
     4501 ctggcagaat ttatgcgcgg actggggttt gtgccgttga ttgccaccac cgccttagca
     4561 acgggtgtgt atgcagttgc gggctttacc tttgtttatg cggtgggcta tctctcgccg
     4621 aatccgatgg ttgcagcggt attaggcgca gtggttattt cggcggaagt cttgctgctt
     4681 cgttcgatcg gcaaatggct gggacgctac ccgtcggtgc gtaatgcgtc ggataacatc
     4741 cgtaacgcca tgaatatgct gatggaagtg gcgctgctgg tcggttcgat tttcgcagca
     4801 attaagatgg cgggttatac cggattctct atcgcggttg ccatttactt cctcaacgaa
     4861 tccctgggcc gtccggtaca gaaaatggcg gcaccggtcg tggcagtaat gatcaccggt
     4921 attctgctga atgttcttta ctggcttggc ctgttcgttc cggcttaagg aaggcaccta
     4981 tgaagacgtt tcctttgcaa agcctgactc ttgctgaagc gcagcaaaag cagtttgcgc
     5041 tggtggacac tatttgccgc cattttccag gcagcgagtt tcttgctggc ggtgatttag
     5101 ggttaactcc aagtctgaat caaccgcgta ttacccaacg ggtggagcag gtgctggctg
     5161 atgcatttca cgcacaggcc gcggcgctgg tgcagggcgc ggggactggc gcgattcgcg
     5221 ccgggctggc ggctttgctc aaaccggggc agcgtcttct ggtgcatgac gcgcctgttt
     5281 acccgacgac acgggttatt attgagcaga tggggctgac gcttattact gctgatttca
     5341 atgacctgtc ggcactgaag caggtcgtcg acgagcaaca acctgatgcc gcgctggtgc
     5401 agcatacgcg ccagcagccg caggacagct atgtgctggc agatgtgctg gcaacgttgc
     5461 gcgcggcagg tgttccagcg ttaaccgatg ataactatgc ggtgatgaag gtagcgcgaa
     5521 tcggctgtga atgcggcgcg aatgtctcga cattctcctg cttcaagctg tttgggccgg
     5581 agggcgttgg tgcagtggtt ggcgatgccg atgttattag tcggattcgc gccacgcttt
     5641 actccggcgg tagccagatc cagggctcac aggcgctgga agtcttgcgt ggtctggtgt
     5701 tcgcgccagt gatgcatgcg gtgcaggcgg gagtatccga acggttgctg gctttgctta
     5761 acggcggcgc ggtggcggaa gtgaaaagcg ccgtcattgc caatgcgcag tcgaaggtgt
     5821 tgattgtcga gtttcatcag ctgattgccg ccagggtgct ggaagaagcg caaaagcgtg
     5881 gcgcgttgcc ttacccggtc ggtgcagagt cgaaatatga aatcccgccg ctcttctatc
     5941 gactttcagg aacgtttcgc caggcgaatc cacaactcga acattgtgcg attcgcatta
     6001 acccgaatcg cagcggtgaa gagacggtgc tgcggatttt gcgtgagagt attgcgagta
     6061 tttaatgctg gatagcaccg ttcggcaatc aagctaaatt ccgcactgaa cggtcccctc
     6121 gcccctttgg ggagagggtt agggtgaggg gaacgtgcgg gatgtaaaat tcagtgcgcc
     6181 agcatcgccg gatgcgatgc tggcgcatct tatccggcct acggttaatg tcttttatta
     6241 tcaatcgtta aggtgatgcg attggcgcgg cagaagagtt cggagacgtg caccggtttg
     6301 tcattctgat caaaggcgat tttgctgatg cgaaacaacg gttcgcccag ttcgcatttc
     6361 agccattttg cctgcgggcg ggtggcggca aagatatcga tggtcttttt gtcgctgacc
     6421 actcgcgtgt caaaacgctc ctgaaataac tgataggtgg agcttccttc gacgtaaatc
     6481 tcatcaaagt caggataacg cgacagcggg atccatgaac tatcaataaa cagcggctct
     6541 ttatcgagat acatcacccg gcagagatgg aacacttcgc tgtttccggg gatgttcagc
     6601 ttttcgcaaa acggcgcggc gctgacccgt tcctgttcga tcactttctc tttcgtcgac
     6661 ttgccttgtg agacgccaaa atcggtaaaa ccactgacag taagcagggc gttttcaact
     6721 ttctggcttt gtacaaaggt gccttttccc tgccagcgga tcagtacgcc gtctgccact
     6781 aagtcgctga tggctttgcg aatggtaatg cggctgacgt tatattgtgt acaaagctcg
     6841 ttttcggtag ggatctgttg cccggcctgg taaaccccct gcgcgatatc atccagcagc
     6901 cgctggcgga cggtagcgta gaggagttga tgagagtagc ggtccgtagc tgacataatc
     6961 gaccctgaaa taaagaatga attactcgga ttatagggtc gttttccggc aagtaacagc
     7021 acgttggcgt gaagatgctc acgccaacgt tatacctacc aggcaccgtg gtactgaatg
     7081 gttttcgccg cgcacgccgt tccctgcgcc atcgcctgcg gtaatgtcat ccccgcagac
     7141 cagccgcaaa ggaatccggc aatgaacgaa tctccggcac ccatggtgtc gataaccgtc
     7201 accggttcag gagcctgacg ccagaactgc gcgccatccc aggcaatgct gccgttttca
     7261 cccagcgtga caatcactgt tcctgcgcca cgggcaacaa tcgctttcat cttcagacgc
     7321 agcgcttcgt cttcttgcgg tgcggaggca aaggcaaaat cgagatgcgg caccagtgtc
     7381 tgccagagcg ggctgtccca cttgtcggag aagtcgaaag cggtaagttt gcccgcagcg
     7441 tgcagctgtg ggaatgcgtc ttccgcatgt ccccagattg ccgcgtgcac aatgtcatac
     7501 tgcgccagcc aggcgtaatc ctcttcactc agggcaaagt cggccatcac gccttcggtg
     7561 tagtcgccaa aaacgcgatc attgtcgtgc agttccacct gagtttgtgc ggtaacgccg
     7621 tgtttcgtat ggacatggct gatatcgacg cccatgcggg cgagatcctg cttcagcttt
     7681 gtgccgtagt catcgtcacc cacccaggta atgcatcccg gctgtatgcc gtagcgagtg
     7741 cagtacaccg ccacattgac cgcattaccg ccagaaaacg ctttattcag ttgcgggtag
     7801 atatcgacgc agttatcgcc gattgtcgcc agggttttca tctctcatcc tccggcagca
     7861 gcgcgcgaaa gcgttccagc gcctggcggg catagagtct gggctcgttc atatacatcg
     7921 tcaccagctc caccgtagag taaccctcat agccccgatc aataatatcg cgcatcagtt
     7981 cccgcagcgg cattttgcct tctccaggaa tgtaatgcgt gtcgctggcc ccgtcgctgt
     8041 cgacaatatg cagatgacgt aatttatcgc ccagtttgtc gaaataactc atcaccggtt
     8101 ccgcctggac atacggcgcg caaatgtcga ccatgctgaa caggcgcggc gaaggcacca
     8161 gcgccagcgc atgaagcaca tcattagcat tacacacgac gttcgattca tacggcgtta
     8221 acggttcgag gatcagatcc atgccgatgt tttccgcgta ctcacacagt tcgcttaagt
     8281 tctctgccag ccgcccccag ataacgttag gcggcgtgag atagcccgcg tgggccgcag
     8341 aaatcagcgt ataacccgcg ttcatctctt ttgccatatc tatcgccagc ttgatcatgt
     8401 ccaggctttc acgacgcata tgttcatcgc ccagcatcat gttatacgga tagccgttgg
     8461 tttctggcgt gtaaccgata atcggcatct gatacgtctg cgccagcgcc ttgatttgtt
     8521 tgatgccgcc cgcttttaag tccggcgcga acgcgtgcgg gcgaccgccc caaatttcga
     8581 tgccgtcgta acccagctcg cttgcatcac gaaatgcgtg ttcaataggc agccgctggt
     8641 ggccgcaggt aaacatacct gttttcatcg ggtaactcct tgcgtaaatc cggcccgttg
     8701 ccgggccggg agagggatta atattccacc agaccaccgt aatagcggcg ttcatccggg
     8761 ttgtgatctt tgtaaataga caggtagtag cagagccact ccattggcac gaacatcagg
     8821 aacggtgcca gccacgggtg cagcccttgc gaaatttcgg cgtaatcgat gacgatcacg
     8881 ttgtcagtac gctgtttaac aaagttaatg gcgcgttcgg tggtgtggcg actttcatca
     8941 ttgccgagca ggaacaggaa cggaacgccc ggttcgacaa tctccagcgg gccatggcgg
     9001 aactctccgc tctcaatcac gcagccgtgc gtccaggtaa attccatcag cgttacaatg
     9061 ccttctttgt aacccagcgg acgcagcgga cccgcagcaa cggtataaat catcggccac
     9121 tggctggcca gttcaccaag ctggcggcct ttttcttccc aggtgcgtac cagatgaccg
     9181 agcgcattcg gcaactgctt gagatcgttt ttgatcttgc cgatttccgc gttcggcgcg
     9241 aggcgggtga tcatctccag caccacgctg tagcagagca gcaggtgaat ttcccagata
     9301 cagtcggcct gataatcaat gctaaattcc gccgccgagg taatcgggct gtccgcgcgt
     9361 ttggtgaacg ccgcagtgag tgctccgcag gcccggccca gctccagcgc tttgattacc
     9421 tcttccgttt taccgtagtc agaaacgcca atcaccgcgc aacgatcgtc gaggcgatac
     9481 ggggtgttat cgcagaactc ccagccggaa atggcgtaga cctgaagatc ggaaaagcga
     9541 tccgccagat gtttcgccgt ttgcgccgcg ttgagtggcg agccgcaggc aacgaaataa
     9601 atacgatcaa tgtcgcgctt caccatctct tccacgatgg cgtgcaccag cggaacgtca
     9661 tggctgagaa ctttttccac ttcctgaacc atattttcgg tgaccagaaa gtccacggtg
     9721 cttttatcaa tatccaacat tttattttct tcagataaca ttacgacaaa gcgttgagct
     9781 ggcggctacg cttcgcccag aaggcgtaag caggcagacc agtagcaata acgatggcgg
     9841 cgcagataag gccgggaatc ggtgcccaga caaaggttga ggcgaccaga atgaggcttg
     9901 acgcaatggc gagggtggtc atcagcccga aagccggagt acgccacagc ggtttgtaat
     9961 cgtcgcgttt acgacaccag atgatggagc cgaaggtgag ggtatttttg aaacacatca
    10021 ccagggtgaa ataacccagc aggctggtga gatcggaaac gaagatgaag aagatcccca
    10081 gcgccccttg caggatgatg gagacatccg gcgtgttgta tttcggatgc gcatggccga
    10141 agcatttaaa gaacaggttg tctttcgcca tcgcgtattc cagccgcggc tggtacatca
    10201 cgcagctgga aagcgaacca agaatgacga tcatcgccgt gatggcaaca aagagcccag
    10261 cggtgctgcc gagtgcgggg atctaggtca gggcgtcgga aatcggcgtt tcagaattgg
    10321 cgagtttgtc gaagggcatc aggccggaaa tcaccagcgc cagcagggtg tagagcacca
    10381 gaaccagcag acaggaacca atcagcgctc gtggcatggt ttttccgggg tttttaattt
    10441 cgccggtcat ataacagata gaggccatgc cggtatacga ccaactggtg gcagagatcc
    10501 ccgccagcag cgccataaag ctgcccgttg cgccaatcgc agtggtggta agggcggcaa
    10561 aattctccgc tttaaaccag aagatcccaa ggccaatgac gatagtgaac gggataattt
    10621 tggcgatggt aattagcgtc tgaaacgctg cgccgccttc aacggagcgc aggtgtagca
    10681 acataaaggc gataattaat ccggcggcga taaatttacc gagcaacgga tcgataggcg
    10741 ttaaaaagcc aagattgctg acaatcgcca gcgccataat cgacaatgac ggcgcatcgt
    10801 tggcccagaa gctggcccag ccggagagga aagccagcgg tcggcttccg gcatttttca
    10861 gataaacata atctgcgcca ttttccggat aagcggtgga tagttccgca tagacgcaca
    10921 tttgcgggat cacaattaac ccgccaatga caaacgcgag caccgtaagc cacggcgtgc
    10981 ccgctgcttt tgccacttca cccacagata caaaaatacc ggagccgacg gttgtcccga
    11041 cggcgattgc aagaacggcc caaaatccga gcttgcgttg gagttcctgg cttcccatag
    11101 cgataccttt ttcttgtcca atacgttgta ggggagagtt tgcagccatt taaaaggtaa
    11161 catgacaatg catgatgaat ataacaatat attatgttat gtgatgtgga ggaagatcac
    11221 aaaatttcgc acaggatcgc gctgtggcta atggatgtag ttataaaatt gaatttaaag
    11281 tgaaaatatt tttacgggcg ggggcaagaa ggacatagaa acaaatacgc cctcggaaaa
    11341 tccagagggc gtcgggcaat taaaccggtg ttagccgatt tctgtcagag acttactgtg
    11401 cagtaggacc gcaaccgcca atgattttag aaatagaaat tgccgggtgc agcaggtagt
    11461 cgtaagagca gttcttatca cggttttcaa tatgaccagt acatgcagtt aacgtagcca
    11521 gtactgcacc taccagtgca actttaataa atttgttcat ttacattccc tatgtattat
    11581 atattaatta atattcattg attatatgaa tataagcaga ctgctcgtta agaacgatac
    11641 aggaatattt aattggcgtc agtgagcgaa tgacttaatt ttgtaaatcg ctgtaacggt
    11701 gaatataacc cattagcaga taatatttat taaggggtta tgttttattt gctgaatata
    11761 taaaaagagt cattaagaag gccagaattc tctggcccgg gttaaattaa tgattggaga
    11821 accagttcag tttatcgcgc aggccaacaa cccgaccaat aataattagc gatggactgt
    11881 tcatttgctg agcaagttca ccgagttgcg tgagcgtacc gtcaatcacg cgctgcgtga
    11941 ctgccgttcc gttttcgaca attgccaccg gcatttcgcc aggcataccg tgttcaatca
    12001 gcttttgctg aatagtcgcg gcctgattca accccatata gaacaccagc gtctgttttt
    12061 ctgccgccag gttttcccag tccagctcgc caccggtttt taagtgtccg gtaattaagc
    12121 gtacgctctg ggcataatcg cgatgcgtga gtggaatacc cgaataggca gagcaaccag
    12181 aagctgcggt aatacccgga accaccgaga acggaatacc cgcgttgcac agtgtttcca
    12241 gctcttcgcc accacggcca aaaataaacg gatcgccacc tttcagccgc accacgcgtt
    12301 tgcctttttg cgcttcccgc agcaggatct ggttaatctc ttcctgcggt acacagtggt
    12361 atcccgcgcg tttgccgacg aaaacgcgat cagcatcgcg gcgtaccaga ttcataatat
    12421 cgtcagaaac cagacggtcg tagaccacca catctgcctg ctgaatttgt tgcagtcctt
    12481 tcagcgtcag cagcccggca tcgcccggac ctgcaccaac cagcaccact tcaccgcgat
    12541 ggtcgagcgg ttcgttgatt aactgttcgg tcgtttcagt aatggctttc tgatcgttgt
    12601 ttgccagcga ctgcgccagg cggtcgttaa cgaacaattt ctcccagaaa cggcgacgct
    12661 cactcatcgt ggcgaactgt tgtttcactc gcccgcgtaa ttgcccggca tatttcgcca
    12721 cctggcccag atgcaacggc agcagtgatt cgagtttttc gcgcaacagg cgcgccagaa
    12781 ccggagaggt gccgccagag gagaccgcta ccattagcgg tgagcggtca ataatcgacg
    12841 gcataataaa gctggcggct ttcggcgcat caactacgtt acagaagatg cgacgagctt
    12901 cagcggcttc gctgacgcgc tggttaagcg tgtcatcatc cgtcgctgca atcgccagcc
    12961 agcaggtgtc gagaaggctt tcatcaaatg gcccttcgac gagggttaac atgcctgcat
    13021 ctgcccatgc ggtgaactgt ggaataaacg ctaatgcatt caccgttaag cgagcgcctg
    13081 cgtctaacag caaccttgct ttgcgttccg cgacatcacc accgccgaca atcagacagt
    13141 cgcgatcgcg taattggcaa aatataggca aatgatccac gtaaaaaccc cttagtaatt
    13201 aaccggcagc tgtttcagtt tgattaaatt tgtccgcaac cggacgattc gctttcggcg
    13261 tagcatacca ataacccaat cccatgaata cggcacctga taaagtatta cccagcgtca
    13321 cccacagcag gttatgacca atacccgcca gcgtgtaggc ttcgctgtgg ttgccgaacc
    13381 aggagagcgc gaacagcgtc atgttagcga tagagtgctc gtagccggac gcgataaatg
    13441 ccagcagaca ccaccagata gcgataaatt tcgccgcccc ttcagtgcgc agcgccatcc
    13501 agattgccag gcaaaccagc cagttacaca atgcaccttt gaagaacagt accattgccg
    13561 gtgcagtggt tttagccagc gcgacggagt gaacgatgct ggtatctacc ggcagcaggc
    13621 taccgccgcc ccagctatag agcatggcaa cgaagacgga accgaccagg ttacccagcc
    13681 aggtttgcgg caggattgcc cacatttgcc cgtggctgat gctgcccgct tttaccccaa
    13741 aggtgaggaa catggtgtgt ccggtgaaca gttcagaacc ggcgataatc accagcgtta
    13801 aggcgatacc aaaggtcgcg cccatcacca gcggacgtac ggacgggtcg agcaaattac
    13861 cgagcgtgaa aatcaggatg atcccaagac ccacatatgc gcccgccatg gcggagctga
    13921 cccaaaagcc gagcgggtta tttgccgaca ggcgtgcaat gcgcgcagca ttagccgcac
    13981 acttattaat agtgtctgta aacatttgat tatcctttaa aaaataaaaa caaaaaataa
    14041 aacaaaaagt ttaaaaaatg tcctgcaata taccgaaggt cattacagga ccatgcaaaa
    14101 aggggaggca ttgcgcctcc cgttaaaaca ttaaccgcgc agctgcacca cgccgtcttt
    14161 cactcgtgct tcgtaatgtt tgacggaaaa ctgttcgtct tccatgcaca agccatcact
    14221 taagcgaaaa cgctgttttt tcagcgggct ggcgacccac agctcgccct ggtgttccgc
    14281 aatcagcccg cgtgatagca cgctggactc gaagaacgga tcaatgttgc tgatcgcaaa
    14341 cacctggtcg ctgtgatacg ggcggaaaat cgctacctgc ttgtcaccta acagtgcgca
    14401 gacgccggtt tcaggcagga tgtcatcgat tttgcagatg tctttccact ggctcatgcg
    14461 ttgtcctcca ccagagttac cgggatacgt tcatacggcg ttgccggacg gtgctgttcg
    14521 cgctctggca ccatctgcac gttcgggtca cgcttgtcgc tgttgatgaa gtgtttgaag
    14581 cgagtctgcg cggacggtgt attgaccgtt tcagtccact cacacactac cgcttcacgc
    14641 aggcgcgcca tctcttcttc cagatgagcg ttcagcccca gtttgtcgtc aatgatcacc
    14701 gctttcaggt aatcgatgcc gccttcgagg ttttctaacc acggcgcggt acgcgtcagt
    14761 ttgtcggcgg tacggatgta gaacatcatg aagcggtcga gatatttgat cagcgtttcg
    14821 cgatcgatat ccgctgccag cagatccgca tgacgcggtt tcatgccgcc gttaccgcaa
    14881 acatacaggt tccagccttt ttcagtggcg ataataccca cgtctttacc ctgagcttct
    14941 gaacattcac gggtacagcc ggagacaccg aacttcattt tgtgcggcgt acggatgcct
    15001 ttgtagcggt tttccagttc cacgccgagg ccgacgctat cgccaacacc gtagcggcac
    15061 caggtgctac ccacgcaggt tttcgccata cgcagtgctt tcgcataggc atgaccggtt
    15121 tcgaagccag cttcaatcag ctgacgccag atttccggca gatcgtcttt ctgtgcgcca
    15181 aacatcgcca ggcgctggga gccggtgatt ttggtgtaga gattaaattc acgcgcgata
    15241 cgacccactg ccatcagccc ttccggggtg atttcaccgc ccggagaacg cgggatcacc
    15301 gagtaggtgc cgtctttctg gatgttagcg aggaagttgt cgttagaatc ctgcagcgga
    15361 gtatgttccg gcttcagaat gtattcgttc cagcaggagg ccagcagcga accgacggtt
    15421 ggtttacaaa cttcacaacc gtagcctttg ccgtgtttcg ccagcagttc ttcgaaggtt
    15481 ttaatgcctt caacgcggat caaatggaac agttcctgac gcgaataagc aaagtgttcg
    15541 cacaggttgt tgttaacttc gatgccctgt ttcgccagtt ccgcgttcag tacctgagtg
    15601 accagcggga tacagccacc gcagccagta cccgctttgg tttcagcttt cagcgccgca
    15661 actgtgtggc agcctttgtt gatggcagca atcagatcac ctttagtgac gtcgaagcag
    15721 gagcagattt gcgcgctgtc cggcagttta tcaacaccga tagacggctt accgctaccc
    15781 gagtgcgccg gcagaatcag agaatccggg ttttccggca gctcgatagc gttcagcacc
    15841 agttgcagca ggttgccgta gtcgctggta tcgcccacca gtaccgcacc gagcagggtt
    15901 ttgttgtctt cgctgacaat caggcgtttg tagatctctt tactttcgtc gaggtaaaca
    15961 tagctacgtg cgccaggcgt gcgaccgtgc gcatcaccaa taccacctac gtctacgccc
    16021 agcagtttca gcttggcgct aaggtcagca ccttcaaagg cgttttcgct acccagaata
    16081 tggtcaacgg cgacctgcgc cattttgtag cctggtgcta ccagaccaaa tacacggttg
    16141 ttccagcttg cgcattcacc gatggcgtag atatccggat cggaagtctg gcaggaatca
    16201 ttaatgacaa tccccccacg cggagcaacg tccagaccac actgggttgc cagcttatcg
    16261 cgcggacgga taccggtaga gaagacgata aagtcgactt ccagttcgct gccgtcggca
    16321 aaacgcatgg ttttacgcgc ttcgacacct tcctgcacaa tctcaagggt gtttttgctg
    16381 gtgtgaacgc gcacgcccat actttcgatt ttgcgacgca gctgctcgcc acccatctga
    16441 tcaagctgtt ccgccatcag cataggggca aattcgataa cgtgggtttc aatacctaag
    16501 tttttcagcg cgccagcggc ttccagacct aacaggccgc caccaacaac ggcaccgcgt
    16561 ttgctgcgac gggcgcagga ttcaatggcg ttgaggtctt caatggtgcg atagacaaag
    16621 cagtcctgag tatcagaacc tttgattggt gggatccacg ggtaggaacc ggttgccatg
    16681 atcagcttgt cataaaaaac ggtacgtccg gcgctggagt gaatcacctt ctcctgacgg
    16741 ttgatggtga tagcgcgttc gccgaccaga actttgatgc cgtgtttctc gtagaagcct
    16801 tcgcgcacca gcgacagctc ttcggcggtg tggtgagaga agtaagatga gaggtgtacg
    16861 cggtcataag cgatgcgcgg ttcttcacag aaaacggtaa tatcaaagtt ggccgcatca
    16921 gatttatcaa gaagatcttc gataaagcga tggccgacca taccgttacc gataattgcg
    16981 agtctgactt tgctcatttt tgcctcgatt tcttttctat taccgcctac cttaacgatt
    17041 cagcaacccc gcttattgat gtaaatcaaa ttcaccttta tatactcctt aaggggtata
    17101 ttgctgattt gtataaattt tcttaagtca cggaattgtc tgacatttca tgtttgtgca
    17161 aaaaatggac aaaatgaacg ggtttataga aatagatgca cgtagcgggg gaagagagga
    17221 aaaggccgga cgacgatagt gccgtccggc attagcaatt aatgagaggt cagggtgtta
    17281 tgctgacggt gacggctgac gaaacctaac aggaagcaca tcacaaagac gacagcgtac
    17341 agaccgtttg ctgtaagcag tgccgcctgc ggaccgctat gttcaacaat cgtgccggta
    17401 accacaaagg tcaacatagt accgatggtt ccgcaggtca ggacaaagtt aaccagtttt
    17461 ggcgacggta ctttggtctg ctgtgaaccc agggtgatga tggtggtata gatcgcgctg
    17521 gagaagaagc ccagagcgag aattgaccac gccatatgta ctggtgttcc ggtgttaaag
    17581 acgtacatca gaatcgcagc cagaccagcc agtacggtca gaatgcgttg caaatcaaag
    17641 aagcgaagaa taaagctgaa cgcccacatg ccgaccatgt atgacatcca gaagttactc
    17701 accagcgtgc ccgcgtcgtt caggctcatg cccaggcctt tggcatactc aggcacccag
    17761 gagataaaac ctaactgacc gaggatgtag cacagcgccg caacagagag aaacagtacg
    17821 ccgatccccc acttttcttc tctaccggag catccgtttt tggtgcacgt ttgcccagtg
    17881 ccgggaactc acagccgaag gtcagaataa aaatagcgac atacaccagc ccgatgcagg
    17941 cataaaccca gtaccactca atgctgcgcg ccagtagaaa cgcggcgatc attgggaaaa
    18001 tcatcccagc catactgaag aaggagtcag taaataacag gcgggaaccg cgctgacgcc
    18061 cttcatacat ttgtgttatc aggaatgtac caatcgacat agtaatgccg ctgaccaccc
    18121 cgagaatgaa catcgccgtc gagaacagcg ccaggctgtg gctgaacatc aaaccggcaa
    18181 ccgccagcac catcaggaga aagccaaaac gtaactgcgt tttcaacggg acgatttcca
    18241 tcagccaggc gttgaggaag atagagatta aaatgccggc gttgaggaag gtgaaggtgt
    18301 tactcatact ggaaacaggc agattgaaat aatcggcgat atttcccatc accatcccgg
    18361 tgacaataac caacgcacca gtcagtgcgt aggagagaaa gctaatccat gtgagcttga
    18421 tgcgattgct gttagtcatg attggcctgc gttcaaaaat aaaatggcat agcgggatat
    18481 gccgcgagcg ggcgatttta ggtgatttta tgatctgttt aaatgtttta ttgcaatcgg
    18541 ttgctaaatt gcattttaag aggtgatttt gatcacggaa taaaaagtga tcgtcaggtt
    18601 acatatattt cagatacgta aaattaggta aagggatggc cttgttcttg aaggctattt
    18661 agaatctctt cacttgcttt ttttctgctc tgtttgttaa ggaaatctca tgttcaaatc
    18721 gaccctggcg gcgatggctg ctgttttcgc tctttctgct ctttctgctc tttctcccgc
    18781 agcaatggca gcgaaagggg acccgcacgt attgttgaca acctcagctg gtaacatcga
    18841 actggagctg gataaacaaa aagcgccagt gtctgtgcaa aactttgtcg attatgtgaa
    18901 cagcggtttt tataacaaca ctacctttca ccgcgtcatt cctggcttta tgattcaggg
    18961 cggcggtttc accgagcaga tgcagcagaa aaaaccaaac ccgccaatca aaaatgaagc
    19021 cgataacggc ctgcgcaaca cgcgtggcac catcgcgatg gcgcgtaccg ctgacaaaga
    19081 cagcgccacc agccagttct ttatcaacgt tgccgataac gccttccttg accatggtca
    19141 gcgtgatttc ggttacgcgg tatttggtaa agtggtgaaa ggcatggacg ttgccgataa
    19201 gatttcccag gtgccgactc atgatgttgg tccgtaccag aatgtgccgt caaaaccggt
    19261 agttatcctt tccgctaaag tcc
//
LOCUS       DAZOYF010000070        18216 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZOYF010000070 DAZOYF010000000
VERSION     DAZOYF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18216
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.070"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="ACHUOF_002296"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="ACHUOF_002296"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR0611458.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="ACHUOF_002297"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="ACHUOF_002297"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR0611459.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..3000)
                     /locus_tag="ACHUOF_002298"
                     /pseudo
     CDS             complement(1728..3000)
                     /locus_tag="ACHUOF_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3183..4388)
                     /locus_tag="ACHUOF_002299"
     CDS             complement(3183..4388)
                     /locus_tag="ACHUOF_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0611460.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4488..4844)
                     /locus_tag="ACHUOF_002300"
     CDS             complement(4488..4844)
                     /locus_tag="ACHUOF_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR0611461.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4980..5087)
                     /gene="rprA"
                     /locus_tag="ACHUOF_002301"
     ncRNA           complement(4980..5087)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHUOF_002301"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="ACHUOF_002302"
     CDS             complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="ACHUOF_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR0611462.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6774..9830
                     /locus_tag="ACHUOF_002303"
     CDS             6774..9830
                     /locus_tag="ACHUOF_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR0611463.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9827..10237
                     /gene="menI"
                     /locus_tag="ACHUOF_002304"
     CDS             9827..10237
                     /gene="menI"
                     /locus_tag="ACHUOF_002304"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR0611464.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10337..10525
                     /locus_tag="ACHUOF_002305"
     CDS             10337..10525
                     /locus_tag="ACHUOF_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR0611465.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11073..11441
                     /gene="sufA"
                     /locus_tag="ACHUOF_002306"
     CDS             11073..11441
                     /gene="sufA"
                     /locus_tag="ACHUOF_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR0611466.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11450..12937
                     /gene="sufB"
                     /locus_tag="ACHUOF_002307"
     CDS             11450..12937
                     /gene="sufB"
                     /locus_tag="ACHUOF_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR0611467.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12947..13693
                     /gene="sufC"
                     /locus_tag="ACHUOF_002308"
     CDS             12947..13693
                     /gene="sufC"
                     /locus_tag="ACHUOF_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR0611468.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13668..14939
                     /gene="sufD"
                     /locus_tag="ACHUOF_002309"
     CDS             13668..14939
                     /gene="sufD"
                     /locus_tag="ACHUOF_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR0611469.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14936..16156
                     /gene="sufS"
                     /locus_tag="ACHUOF_002310"
     CDS             14936..16156
                     /gene="sufS"
                     /locus_tag="ACHUOF_002310"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR0611470.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16169..16585
                     /gene="sufE"
                     /locus_tag="ACHUOF_002311"
     CDS             16169..16585
                     /gene="sufE"
                     /locus_tag="ACHUOF_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR0611471.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16734..17738
                     /gene="ldtE"
                     /locus_tag="ACHUOF_002312"
     CDS             16734..17738
                     /gene="ldtE"
                     /locus_tag="ACHUOF_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR0611472.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="ACHUOF_002313"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="ACHUOF_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR0611473.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4789 a         4403 c         4680 g         4344 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgacag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgtactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagccatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgtcaaa aaagaaggcc
     4141 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
     4381 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta
     4441 tccattcatt attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc
     4501 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
     4561 ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg
     4621 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga
     4681 cgcatagcca acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac
     4741 tgacgccagc tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc
     4801 atggcaaaaa tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
     4861 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
     4921 agtatcttgt gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt
     4981 taaaaaaaag cccatcgtgg gagatgggaa aagactacac acagcaattc gttgtttcac
     5041 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag
     5101 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
     5161 tgcgacaggt actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa
     5221 atatcaatta gcggtgatta acaatcaggc ggcagagccg cctgatgaga aattacttat
     5281 tcggtttctc gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggtggcg
     5341 ggacttcttc cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa
     5401 taaacagacc gatcatcccg aaagcaatca aaccaccaat aacgccagag aggatcagga
     5461 tcagcggtaa atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc
     5521 caaccacacc gctccacact aacaataccg ttccccaggt ggtatcgccg gtccagtaga
     5581 gccagataat cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga
     5641 tcattaacac cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc
     5701 caagcactgc ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct
     5761 gtgccgccag cagcacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc
     5821 gaatgccttg tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca
     5881 gcgcacaatg caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg
     5941 tggtgccaat ataagggcgg actttcgcca tgatcgccgt accccccata tccagcaagt
     6001 tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat
     6061 cgggtaacgt catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga
     6121 tactgttaac cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca
     6181 ccagaacggc gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc
     6241 aggtggcgat aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc
     6301 acagacatgc cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat
     6361 ccctgggctg acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc
     6421 gacgcgacgt gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta
     6481 acaggccgat tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac
     6541 tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt
     6601 tcgttcaccc cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc
     6661 tgcaactcga aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc
     6721 tgaaagaata gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc
     6781 cacagatttc cctggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg
     6841 agcaacaagg ttttaccggc gatgcggcga caagttatgc cgatcgtctg acaatgtcga
     6901 ccgacaacag tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag
     6961 atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc
     7021 cccgcggcgg cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata
     7081 tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg
     7141 aggccggggt gataaaagac caactcaatc agtacctgaa accgttcggc tacttttttg
     7201 cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat
     7261 ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg
     7321 tgttgttggg gggcgatatt ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc
     7381 tgggtaaatc caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc
     7441 agcaacgcca gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg
     7501 atctgcgtca tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg
     7561 gttcagaagg gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta
     7621 aagtgcgccg tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc
     7681 cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc
     7741 tggcgcggga agatattgtc tggcattccg tcagcgagtt gattaccgat atgcctgaca
     7801 aagagatgct cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg
     7861 agcgagttaa tgcactctgt gtgcggcttg atgagctgat tgccagccaa caggcaggtg
     7921 tgattggctg gcaggtgtgc cgcgagctgg cgggggttga acgtatctat gcgatgcgca
     7981 aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg
     8041 aggatacctg cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc
     8101 tcgacagcca cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg
     8161 tccgtccggc actggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg
     8221 atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag
     8281 gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca
     8341 aagtgaaagc ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag
     8401 aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc
     8461 agatccccat tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt
     8521 tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc
     8581 ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg
     8641 atcgcggcgt tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc
     8701 ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct
     8761 cacacgaagt caaagaggcg atgtcgggct gtctggcctg taaagcttgt tcgacccagt
     8821 gccccatcaa aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc
     8881 gttatttacg cccgcttcgc gaccacctcg ttgctacggt cgagagctac gcaccgctga
     8941 tggcacgcgc gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct
     9001 cggaaaaaca tatcggcatg gttgatttgc cactgctgtc ggtcccctcg ctacaacaac
     9061 aaatggtggg gcatcgctcg gcaaacatga cgctggaaca gcttgaagct ctcaatgcag
     9121 agcagaaagc gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc
     9181 aagtggtggc ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc
     9241 cattttcgcc aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga
     9301 cggcgaaaaa gacggcggat ttcctcaacc gtatggctaa gcttgggatg ccaatggtgg
     9361 gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg
     9421 aacgtggcga gtttaacgtc ttacttgcga atgaatggct ggcaagcgca cttgagtcac
     9481 atccagtggc tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaggtta
     9541 ccgctttgcc gggcgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac
     9601 tggaaaatgt cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa
     9661 accatgaaaa ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc
     9721 cgcgtaaccg ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag
     9781 gcacgggggt acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg
     9841 gaaaatcacc ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga
     9901 tattcgcttt gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg
     9961 gacaaagcag cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat
    10021 cggttccgtt gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat
    10081 caatgctaat cacgtccgct cggcacgaga agggcgggtg cgcggcatat gtaaaccgtt
    10141 gcatctcggt tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt
    10201 gtgctgttcg tcacgattga cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac
    10261 gcatattgct tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc
    10321 taaaagcgag gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt
    10381 acgccgtgac cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga
    10441 acttgaattt gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt
    10501 cgcctggcgt ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc
    10561 tggattaacc tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata
    10621 ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat
    10681 tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg
    10741 gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct
    10801 gcactttttt acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac
    10861 agccttaatg ctccttagat aataaccatt atctaacaat gagataccta attcttagcg
    10921 tgcctgttaa cccacacatc aggggctatg cttattaaaa gcaggcaaaa agaatgtttt
    10981 acggtaaagc ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt
    11041 atacgctgaa agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc
    11101 cacaggattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg
    11161 agctggtggc aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct
    11221 gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt
    11281 ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaatc gatgccgttt attgatggca
    11341 cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag
    11401 cccagaatga atgtggctgt ggcgaaagct ttagggtata ggcggtacta tgtctcgtaa
    11461 tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaact ataaagaagg
    11521 attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg
    11581 cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta
    11641 tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa
    11701 ttatcaggat tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc
    11761 gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga
    11821 ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc
    11881 cattttcgac tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat
    11941 tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct
    12001 cggcaccgtg gtgccgggga atgacaactt ctttgcagca cttaatgcgg cggtagcctc
    12061 tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta
    12121 ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc acaattctgg tggccgacga
    12181 agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt
    12241 gcacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt
    12301 acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg
    12361 tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc
    12421 gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta
    12481 ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat
    12541 cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa
    12601 cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca
    12661 gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg
    12721 tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca
    12781 actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa
    12841 cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa
    12901 actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa
    12961 agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg
    13021 tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc
    13081 aacgcttgcc gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa
    13141 agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca
    13201 gtatccggtg gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc
    13261 agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga
    13321 agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg
    13381 tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga
    13441 gttatgcatt cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc
    13501 cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca
    13561 acgcattctc gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt
    13621 gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac
    13681 cgaacagcag taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac
    13741 gctcaccgca agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta
    13801 aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagtcag tttgtcagca
    13861 ttgcgggaga tatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc
    13921 ggctggtgtt tgtcgatgga cgttacgtgc ccgcgctgag cgatgcaatt gaaggcagcg
    13981 gatatgaagt gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag
    14041 tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg
    14101 gtcaacggcc ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag
    14161 aggtgaacac tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg
    14221 tgatcgaaca ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta
    14281 tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca
    14341 gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata
    14401 gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa
    14461 acagcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc
    14521 gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg
    14581 tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca
    14641 aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata
    14701 caaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg
    14761 ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg
    14821 cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg
    14881 ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac
    14941 tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt
    15001 gccgctggct tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc
    15061 cgaggccgag ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag
    15121 cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc
    15181 ccgttcggcg gaagagctgg tgtttgtccg cggcacgacg gaagggatca atctggtcgc
    15241 caatagctgg ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga
    15301 gcaccacgct aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg
    15361 tgtgatccct ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga
    15421 tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc
    15481 actggcggaa atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc
    15541 tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt
    15601 ctccgggcat aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt
    15661 gttgcaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag
    15721 tgaaggcact acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg
    15781 gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat
    15841 agccgagtat gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga
    15901 tctcactctc tatggccaac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca
    15961 ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca
    16021 tcactgcgct atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct
    16081 ggccatgtat aacacccatg aagaagtgga gcgtctggtg accggcctgc aacgtattca
    16141 ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct
    16201 gcgtaatttt ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg
    16261 ccagcgtctg ccagaattac gcgccgaaga cggaagccca caaaatagca ttcagggctg
    16321 ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg
    16381 cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca
    16441 gatgacgccg caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct
    16501 cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg
    16561 cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat
    16621 tgccggatgg tcaatgttta cactgaccgg cacaggagtg ttttggtttc aggtgaacat
    16681 aagcaccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac
    16741 gcgcgtcttt gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg
    16801 cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata
    16861 cggtgcaaga aggggataaa aaccttcagg ctattgcccg acgttttgat actgcagcaa
    16921 tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa
    16981 ctattccttc acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg
    17041 cagagctgcg cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag
    17101 gtattggatt gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc
    17161 ctaacccaac ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta
    17221 aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc
    17281 tcgcgcatgg taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt
    17341 tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca
    17401 gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac
    17461 ctaacgggat gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg
    17521 ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg
    17581 tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga
    17641 gcagtggagc aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg
    17701 aaccagagca agggaatatg ttacgcgcga cgcagtagtg gtaaatagca gacaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcg
//
LOCUS       DAZOYF010000071        18065 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZOYF010000071 DAZOYF010000000
VERSION     DAZOYF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.071"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..331)
                     /locus_tag="ACHUOF_002314"
     CDS             complement(<1..331)
                     /locus_tag="ACHUOF_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR0611474.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLF"
     gene            483..812
                     /locus_tag="ACHUOF_002315"
                     /pseudo
     CDS             483..812
                     /locus_tag="ACHUOF_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
     gene            1060..1305
                     /gene="nrdH"
                     /locus_tag="ACHUOF_002316"
     CDS             1060..1305
                     /gene="nrdH"
                     /locus_tag="ACHUOF_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR0611475.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            1302..1712
                     /gene="nrdI"
                     /locus_tag="ACHUOF_002317"
     CDS             1302..1712
                     /gene="nrdI"
                     /locus_tag="ACHUOF_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFR0611476.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1685..3829
                     /gene="nrdE"
                     /locus_tag="ACHUOF_002318"
     CDS             1685..3829
                     /gene="nrdE"
                     /locus_tag="ACHUOF_002318"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0611477.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3839..4798
                     /gene="nrdF"
                     /locus_tag="ACHUOF_002319"
     CDS             3839..4798
                     /gene="nrdF"
                     /locus_tag="ACHUOF_002319"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0611478.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5153..6355
                     /gene="proV"
                     /locus_tag="ACHUOF_002320"
     CDS             5153..6355
                     /gene="proV"
                     /locus_tag="ACHUOF_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR0611479.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6348..7412
                     /gene="proW"
                     /locus_tag="ACHUOF_002321"
     CDS             6348..7412
                     /gene="proW"
                     /locus_tag="ACHUOF_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR0611480.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTSPVGLLTRPFIK"
     gene            7470..8462
                     /gene="proX"
                     /locus_tag="ACHUOF_002322"
     CDS             7470..8462
                     /gene="proX"
                     /locus_tag="ACHUOF_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR0611481.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            8654..9833
                     /locus_tag="ACHUOF_002323"
                     /pseudo
     CDS             8654..9833
                     /locus_tag="ACHUOF_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9957..10694
                     /gene="ygaZ"
                     /locus_tag="ACHUOF_002324"
     CDS             9957..10694
                     /gene="ygaZ"
                     /locus_tag="ACHUOF_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR0611482.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            10684..11019
                     /gene="ygaH"
                     /locus_tag="ACHUOF_002325"
     CDS             10684..11019
                     /gene="ygaH"
                     /locus_tag="ACHUOF_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR0611483.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11110..11640
                     /gene="emrR"
                     /locus_tag="ACHUOF_002326"
     CDS             11110..11640
                     /gene="emrR"
                     /locus_tag="ACHUOF_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR0611484.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            11767..12939
                     /gene="emrA"
                     /locus_tag="ACHUOF_002327"
                     /pseudo
     CDS             11767..12939
                     /gene="emrA"
                     /locus_tag="ACHUOF_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            12956..14494
                     /gene="emrB"
                     /locus_tag="ACHUOF_002328"
     CDS             12956..14494
                     /gene="emrB"
                     /locus_tag="ACHUOF_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR0611485.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(14558..15073)
                     /gene="luxS"
                     /locus_tag="ACHUOF_002329"
     CDS             complement(14558..15073)
                     /gene="luxS"
                     /locus_tag="ACHUOF_002329"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR0611486.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(15223..16779)
                     /gene="gshA"
                     /locus_tag="ACHUOF_002330"
     CDS             complement(15223..16779)
                     /gene="gshA"
                     /locus_tag="ACHUOF_002330"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR0611487.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(16852..17280)
                     /locus_tag="ACHUOF_002331"
     CDS             complement(16852..17280)
                     /locus_tag="ACHUOF_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR0611488.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(17277..17843)
                     /gene="yqaB"
                     /locus_tag="ACHUOF_002332"
     CDS             complement(17277..17843)
                     /gene="yqaB"
                     /locus_tag="ACHUOF_002332"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR0611489.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4294 a         4698 c         4586 g         4487 t
ORIGIN      
        1 caaataaacc attcaaataa cgttcaagcg caacacgcga actaaagcca tgcgggatac
       61 cgtaatgctc gtgtcgctcg cctgctaaat agagcggaaa ttgctggtaa tgatcgcaag
      121 ttttaatatc ggaagcgggt tcagccagtg ttgaactgcg ttcttttaat gttggatgaa
      181 ttaccagagc ggtacgtccc attcgcgctt cgcgattaac atagacataa ttttccccac
      241 ggcggtaacc atacgctttc tgggttacca catcgacagt aaaaccgact ttttcaagta
      301 cgcgcgccac ctcgtctggt cgtaaataca tatttgatcc tcgttatttt caaccgcggg
      361 cctaccttac ctgattgcgc attcacaacg ctttcagaaa agtccataaa gccgcgatga
      421 acctcagtta agaaaattat ggtctacact gaaaattaca tcgaattcta atgggggatc
      481 atatgtttaa ccgaccgaac cgcaacgacg ttgatgatgg cgtgcaggat atccagaatg
      541 atgtcaatca attagctgac agcctcgaat ctgtattgaa gtcctggggc agcgacgcca
      601 aaggggaagc tgaagccgca cgcagtaaag ctcaggcgtt gctgaaagaa accagggcgc
      661 gaatgcatgg tcgtactcgc gtccagcaag ccgcgcgcga tgccgttggc tgagcggatt
      721 cttttgttcg tgaaagaccc tggtgtagcg tgggtacagc agctgcggta ggtattttta
      781 tcggcgcact gttaagcatg cgcaaatcgt agtgcaaaaa tgataataaa tacgcgtctt
      841 tgaccccgaa gcctgtcttc ggggtttctt tttgcctggt gaatcacaaa aatccccctc
      901 ccccgtcacg ctcatatcca gggtaatttc gatcactatt tgctatatat tgtgtagttg
      961 aatctttttt caactacatc tagtatctct gtatcaacag agagacaacc cgacgcgtat
     1021 catcgcgccg tatcttcatt ttaaacggaa atacgaatca tgcgcataac tatttatact
     1081 cgtaacgatt gcgttcagtg ccacgccacc aaacgggcga tggaaaaccg gggctttgat
     1141 tttgaaatga ttaatgtcga tcgcgttcct gaagcagcgg aagcgttgcg tgctcagggc
     1201 tttcgtcagt tgccggtagt gattgctggc gatcttagct ggtctggttt ccgtccggac
     1261 atgattaacc gtctgcatcc agcgccacac gcggccagtg catgagccag ctcgtctact
     1321 tctccagcag ctccgaaaac acgcagcgtt ttatcgaacg tttaggtctg cccgcggtgc
     1381 gcatcccgct caatgagcgg gaacggattc aggtagacga gccttacatc ctgatcgtgc
     1441 cctcttacgg cggcggcggt acggctggcg cggtgccacg acaggtaatt cgctttttaa
     1501 acgacgagca caaccgggcg ttgcttcgcg gcgttattgc ttctggtaat cgcaactttg
     1561 gtgaggcgta tggccgcgcc ggagatgtga ttgcccggaa atgcggcgtg ccgtggctgt
     1621 accgttttga actcatgggt acgcaaagcg atatcgaaaa cgttcgtaaa ggagtaaccg
     1681 aattttggca acgacaaccg cagaatgcct gacgcaggaa acgatggatt accacgcgct
     1741 gaatgcgatg cttaatcttt acgatagcgc aggtcgcatt cagttcgata aagaccgcca
     1801 ggccgttgac gcctttattg cgacgcatgt gcgtccgaac agtgtgacct tcagtagcca
     1861 gcagcagcgc ctgaactggc tggtcaacga aggttactat gatgaaagcg ttcttaatcg
     1921 ctactctcgc gactttgtca ttacgctgtt tgcccacgca cacaccagcg gttttcgttt
     1981 ccagacattc ctcggggcat ggaagtttta caccagctat acgttgaaga cattcgacgg
     2041 taaacgttat ctggaagatt ttgccgatcg agtcacgatg gtggcgctga cgctggcaca
     2101 aggcgatgag acgctggcgt tgcaactgac cgatgaaatg ctgtcaggac gctttcagcc
     2161 agccacgcca acattcctca actgcggtaa gcagcagcgc ggcgaactgg tttcctgttt
     2221 tttgctgcgt attgaagaca atatggagtc gattggtcgg gcggtaaatt ccgcactgca
     2281 gctgtcgaaa cgcggcggcg gcgtagcatt tttgctgtcg aatctgcggg aagcgggcgc
     2341 gccaattaaa cgtattgaaa atcaatcttc tggcgtaatt ccggtgatga aaatgttgga
     2401 agacgcattt tcctatgcca accaactcgg cgcgcgtcag ggggcaggtg cagtctattt
     2461 acatgctcat catcccgata ttctgcgttt tctcgacacg aaacgggaaa atgccgacga
     2521 aaaaatccgc attaaaacac tgtcgctggg cgtggtgatc ccggatatca ctttccatct
     2581 ggcaaaagag aatgcgcaga tggcgctgtt ttcgccctat gacgttgagc gagtttatgg
     2641 caagccgttt gccgatatcg ccattagcga acactatgac gaactggttg ccgatgaacg
     2701 catccgcaaa aaatacctca acgcccgtga tttcttccag cgactggcag aaatccagtt
     2761 tgagtccggc tatccctaca tcatgtatga agacacagta aaccgtgcta accctatcgc
     2821 cgggcgcata aatatgagta atctctgctc agaaattttg caggttaaca gcgcctcaga
     2881 gtatgacgag aatctcgact atgcccgcac aggccatgat atttcctgca atttaggttc
     2941 gttgaatatt gcgcacacca tggattcccc cgatttttcc cgcacggtag agactgccgt
     3001 gcgcggttta acggcggtgt cagatatgag tcatatccgc agcgtgccgt ccatcgaagc
     3061 cggaaatgcc gcctcgcacg ccatcggact ggggcagatg aatttacatg gttatctggc
     3121 gagggaaggc atcgcttatg gttcgccgga agccctggat ttcaccaatc tctatttcta
     3181 taccatcacc tggcacgcac tgcgtacctc gatgttgctg gcgcgcgaac gcggtgaaac
     3241 cttcgccggg ttcaaacagt cacgctatgc cagtggtgaa tatttcagcc aatatctgca
     3301 aggtaactgg cagccgaaaa cggcgaaagt tggcgaactg tttgcccgca gctgtattac
     3361 gttacctacc cgtgagatgt gggcgcaact gcgcgacgac gtgatgcgct acggcatata
     3421 caaccagaat ctccaggcgg tgccgccaac cgggtctatc tcttatatca accatgctac
     3481 gtcgagtatt catccgattg tggcgaaagt agagatacgc aaagagggca aaacaggacg
     3541 cgtttactac cctgccccgt ttatgactaa cgaaaatctg gcgctgtatc aggacgctta
     3601 cgaaattggc gcagaaaaga tcatcgacac ctacgcggaa gcgactcgcc atgtcgatca
     3661 ggggctgtcg ctgacgctgt ttttccccga taccgccacc actcgcgata tcaacaaagc
     3721 gcagatttac gcctggcgca aaggtatcaa aacgctctat tacatccgcc tgcgtcagat
     3781 ggcgctggaa ggcactgaaa ttgaaggctg cgtctcctgt gcactttaag gaatatctat
     3841 gaaactctca cgtatcagcg ccatcaactg gaacaagata tctgacgata aagatctgga
     3901 ggtgtggaat cgcctgacca gcaatttctg gctaccagaa aaggtgccgc tgtcgaacga
     3961 tattcccgcc tggcagacat taactgtcgt agaacaacaa ctgacgatgc gcgtttttac
     4021 tggcctgacg ctgctcgaca cgctgcaaaa tgttatcggc gcgccttcgc tgatgcccga
     4081 tgcgctcacg cctcatgaag aggcggtatt atcgaatatc agctttatgg aagcggttca
     4141 tgcccgctct tacagttcga ttttctcgac gctgtgccag accaaagatg tcgatgccgc
     4201 ctacgcctgg agtgaagaaa acgcaccgtt gcagcgaaaa gctcagatta ttcagcaaca
     4261 ttatcgcggt gatgatccgc tgaaaaagaa aatcgccagt gtgtttcttg aatctttttt
     4321 gttctattcc ggtttctggc tgccgatgta tttttccagc cgcggaaagc tgaccaatac
     4381 cgcggacctg atccgtctga ttatccgcga tgaagcagtc cacggttact acataggcta
     4441 taaatatcag aaaaacatgg aaaagatatc tctgggacaa cgtgaagagt tgaagagttt
     4501 cgccttcgat ttgttgctgg aactctacga caacgagttg caatacaccg atgagctgta
     4561 cgccgaaacc ccgtgggctg acgatgtgaa agcgtttctc tgttacaaca ccaataaggc
     4621 tttgatgaat ctgggctacg aaccgttatt tcccgcagaa atggcggaag tgaatccggc
     4681 aatcctcgcc gcgctttcgc cgaatgccga tgaaaatcac gatttctttt ccggttcagg
     4741 ctcctcttat gtgatgggga aagcggttga aacagaagat gaagactgga atttctgagg
     4801 gtgttatttt caaaaatatc actacccgca gcagggaaat aattcccgcc aaatagcttt
     4861 ttatcacgca aatattctgt agtgatctac actgatactc tgttgcatta ttcgcctgaa
     4921 actacaatat tcaggcgttt tttcgctatc tatgacaaaa aatatcaact tttctcgatt
     4981 tgctctcagc ccttatatca cggaaaattc cggcgatttg ctcgcatcaa tattcatgcc
     5041 acatttgccc tcaggggttg cctcagattc tcagtatgtt agggtagaaa aaagtgacta
     5101 ttcccattgg gtaatatatc gacataaaca aataaagaaa tcattctatt gcatggccat
     5161 taaattaaaa attaaaaatc tttataaaat atttggtgag catccacagc gagcgttcaa
     5221 atatatcgaa caaggacttt caaaagaaca aattctggaa aaaactgggc tatcgcttgg
     5281 cgtaaaagac gccagtctgg ccattgaaga aggcgagata tttgtcatca tgggattatc
     5341 cggctcgggt aaatccacaa tggtacgcct tctcaatcgc ctgattgaac ccacccgcgg
     5401 gcaagtgctg attgatggtg tggatattgc caaaatatcc gacgccgaac tccgtgaggt
     5461 gcgcagaaaa aagattgcga tggtcttcca gtcctttgcc ttaatgccgc atatgaccgt
     5521 gctggacaat actgcgttcg gtatggaatt ggccggaatt aatgccgaag aacgccggga
     5581 aaaagccctt gatgcactgc gtcaggtcgg gctggaaaat tatgcccaca gctacccgga
     5641 tgaactctct ggcgggatgc gtcaacgtgt gggattagcc cgcgcgttag cgattaatcc
     5701 ggatatatta ttaatggacg aagccttctc ggcgctcgat ccattaattc gcaccgagat
     5761 gcaggatgag ctggtaaaat tacaggcgaa acatcagcgc accattgtct ttatttccca
     5821 cgatcttgat gaagccatgc gtattggcga ccgaattgcc attatgcaaa atggtgaagt
     5881 ggtacaggtc ggcacaccgg atgaaattct caataatccg gcgaatgatt atgtccgtac
     5941 cttcttccgt ggcgttgata ttagtcaggt gttcagtgcg aaagatattg cccgccggac
     6001 accgaatggc ttaattcgta aaactcctgg cttcggccca cgttcggcac tgaaattatt
     6061 gcaggatgaa gatcgcgaat atggctacgt tatcgaacgc ggtaataagt ttgtcggcgc
     6121 agtctccatc gattcgctta aaaccgcgtt aacgcagcag caaggtcttg atgcggcgct
     6181 gattgatgcg ccgttagcag tcgatgcaca aacgcctctt agcgagttgc tctctcatgt
     6241 cggacaggca ccctgtgcgg tgcccgtggt cgacgaggac caacagtatg tcggcatcat
     6301 ttcgaaagga atgctgctgc gcgctttaga tcgtgagggg gtaaataatg gctgatcaaa
     6361 ataatccgtg ggataccacg ccagcggcgg acagtgccgc gcaatccgca gacgcctggg
     6421 gtacaccgac gactgcaccg gctgacggcg gtggtgctga ctggctgacc agtacgcctg
     6481 cgccaaacgt cgagcatttt aatattctcg atccgttcca taaaacgctg atcccgctcg
     6541 acagttgggt cactgaaggg atcgactggg ttgttaccca tttccgtccc gtcttccagg
     6601 gcgtgcgcgt tccggttgat tatatcctca acggtttcca gcaattgctg ctgggtatgc
     6661 cagcgccagt ggcgattatc gttttcgctc tcatcacctg gcagatttcc ggggtcggaa
     6721 tgggcgtggc gacgctggtc tcgctgattg ccatcggcgc aatcggtgcc tggtcgcagg
     6781 caatggtgac tctggcgctg gtgttaactg ccctgctgtt ctgtatcgtc atcggtttgc
     6841 cgttggggat atggctggcg agaagtccgc gagcggcgaa aattattcgt ccactgcttg
     6901 atgccatgca gaccacgcca gcgtttgttt atctggtgcc aatcgtcatg ctatttggta
     6961 tcggtaacgt gccgggcgtg gtggtgacga tcatctttgc gctgccgccg attatccgtc
     7021 tgaccattct ggggattaac caggttccgg cggatctgat tgaagcctcg cgctcattcg
     7081 gtgccagccc gcgccagatg ctgttcaaag ttcagttacc gctggcgatg ccgaccatta
     7141 tggcgggcgt taaccagacg ctgatgctgg ccctttctat ggtggtcatt gcctcgatga
     7201 ttgccgtcgg cgggctgggt cagatggtac ttcgcggtat cggtcgtctg gatatggggc
     7261 ttgccaccgt tggcggcgtc gggattgtga tcctcgccat tatcctcgat cgcctgacgc
     7321 aggccgttgg gcgcgactca cgcagtcgcg gcaaccgtcg ctggtacacc actagccctg
     7381 ttggtctgct gacccgccca ttcattaagt aactctgcac ttgcccggtg acgccgggca
     7441 ttatcaccct gccaaaaaaa ggaataacaa tgcgacatag cgtacttttt gcgacagcgt
     7501 ttgccacgct tatctctaca caaacttttg ctgccgatct gccgggcaaa ggcattactg
     7561 ttaatccagt tcagagcacc atcactgaag aaaccttcca gacgctgctg gtcagtcgtg
     7621 cgctggagaa attaggttat accgtcaaca aacccagcga agtggattac aacgttggct
     7681 acacctcgct tgcttccggc gatgcaacct tcaccgccgt gaactggacg ccactccatg
     7741 acaacatgta cgaagctgcc ggtggcgata agaaatttta tcgtgaaggg gtatttgtta
     7801 acggcgcagc acagggttac ctgatcgata agaaaaccgc cgaccaatac aaaatcacca
     7861 acatcgcaca actgaaagat ccgaagatcg ccaaactgtt cgataccaac ggcgacggta
     7921 aagcggattt aaccggttgt aaccctggct ggggctgcga aggtgcgatc aaccaccagc
     7981 ttgccgcgta tgaactgacc aacaccgtga cgcataatca ggggaactac gcagcgatga
     8041 tggccgacac catcagtcgc tacaaagagg gcaaaccggt gttttattac acctggacgc
     8101 cgtactgggt gagtaatgag ctgaagccag ggaaagatgt ggtctggttg caggtgccgt
     8161 tctccgcact gccgggcgat aaaaatgccg ataccaaact gccgaatggc gcgaattacg
     8221 gcttcccggt cagcaccatg catatcgttg ccaacaaagc ctggaccgag aaaaatccgg
     8281 cggcagccaa actgtttgcc attatgcagt tgccagtggc cgatattaac gcccagaacg
     8341 ccattatgca tgacggcaaa gcctcagaag gcgatattca gggccacgtt gatggctgga
     8401 tcaaagccca ccagcagcag ttcgatggct gggtgaatga ggcgctggca gcgcagaagt
     8461 aatttttatt cgggcggata aggcgttttc gcctcatccg ccgctctgta cacaatgcct
     8521 gatgcgacgc tgacgcgtcc tatcatgcct acaaaccaca tcgcacattt caacaagcta
     8581 tctttcatcc catattcatc aacatccgct attattgatt tccagcttaa tcatcacctg
     8641 atgaacaaaa ataatgacta aacctaatca tgagcttagc ccggcgctga tcgtgctgat
     8701 gtctatcgcc accggtctgg cggtagccag taactattac gcccagccat tgctcgacac
     8761 catcgcgcgt aacttttccc tttccgccag ttcggcaggc tttattgtta ccgccgcgca
     8821 gttgggctat gccgcaggtc tactgtttct tgttcccctc ggtgatatgt ttgaacgccg
     8881 ccgcctgatt gtctcgatga ccttactggc agcgggcggt atgttaatca ctgccagcag
     8941 tcagtcactg gcgatgatga tcctcggtac ggcattaacc ggtttattct cagtcgtggc
     9001 acaaattctg gttccgctgg cagcgacgct ggcttcaccg gataaacgcg gcaaagtggt
     9061 tggcaccatt atgagcggtc tgctgttggg gatattgctg gcacgaaccg ttgccgggtt
     9121 actggcaaac ctcggcggct ggcgcaccgt cttttgggtt gcttcggtgt taatggcact
     9181 gatggcgctg gcattatggc gtggtctgcc acaaatgaaa tcagaaaccc acctcaacta
     9241 cccacagttg ttgggttccg ttttcagcat gttcatcagc gataagatcc tgcgcacccg
     9301 cgcgttgctg ggctgcatga cctttgccaa cttcagcatt ctctggacct caatggcctt
     9361 tttgcttgcc gctccacctt ttaactacag cgatggtgac attggtctgt ttgggcttgc
     9421 gggagctgcc ggagcgttgg gcgctcgtcc ggcgggcggt tttgccgaca agggcaaatc
     9481 gcaccacaac tttcggtctg cttctgctgt tactttcatg gctggcaatc tggtttggtc
     9541 acacttccgt actggcgttg attatcggca ttctggtgct ggacctcacc gtacagggcg
     9601 tgcatatcac taaccagacg gtaatttatc gaatacatcc tgatgcgcgt aatcgcctga
     9661 ccgcaggtta catgaccagc tactttattg gcggtgccgc cggttcgcta atttcagcct
     9721 cagcctggca acatggcggt tgggctggcg tttgtctggc tggcgcgacg attgccctgg
     9781 ttaacttact ggtctggtgg cgaggttttc atcgtcagga agccgcaaat taagcaatca
     9841 gcaaccgttt tcgtggcttt acacttataa gggtgttaag aagcccatca gtctgataag
     9901 gttaagatat tcattcagtc tatttataat attaacaatc gttaagcgta cactctatgg
     9961 aaagccctac tccacagcct gctcctggtt cggcgacctt catggaagga tgcaaagaca
    10021 gtttaccgat tgttattagt tatattccgg tggcctttgc gttcggtctg aatgcgaccc
    10081 gtctgggatt ctctcctctc gaaagcgttt ttttctcctg catcatttat gcaggcgcga
    10141 gccagttcgt cattaccgcg atgctggcag ccgggagtag tttgtgggtt gctgcactga
    10201 gcgtcatggc aatggatgtt cgccatgttt tgtatggccc gtcactgcgt agccgtatta
    10261 ttcagcgtct gcaaaaatcg aaaaccgccc tatgggcgtt tggcctgacg gatgaggttt
    10321 ttgccgccgc caccgcaaaa ctggtacgca ataatcgccg ctggagcgag aactggatga
    10381 tcggcattgc cttcagttca tggtcatcgt gggtatttgg tacggtaata ggggcattct
    10441 ccggcagcgg cttgctgcaa ggttatcccg ccgttgaagc tgcattaggt tttatgcttc
    10501 cggcactctt tatgagtttc ctgctcgcct ctttccagcg caaacaatct ctttgcgtta
    10561 ccgcagcttt agttggtgcc cttgcaggcg taacgctatt ttctattccc gtcgccattc
    10621 tggcaggcat tgtctgtggc tgcctcactg cgttaatcca ggcattctgg caaggagcgc
    10681 ccgatgagct atgaggttct gctgcttggg ttactggttg gcgcggcgaa ttattgcttc
    10741 cgctatttgc cgctgcgcct gcgtgtgggt aatgcccgcc caaccaaacg tggcgcggta
    10801 ggtattttgc tcgacaccat tggcatcgcc tcgatatgcg ctctgctggt tgtctctacc
    10861 gcaccagaag tgatgcacga tacacgccgt ttcgtgccca cgctggtcgg cttcgcggta
    10921 ctgggtgcca gtttctataa aacacgcagc attatcatcc caacactgct tagtgcgctg
    10981 gcctatgggc tcgcctggaa agtgatggcg attatataac ccacaagaat catttttcta
    11041 aaacaataca tttactttat ttgtcactgt cgttactata tcggctgaaa ttaatgaggt
    11101 catacccaaa tggatagttc gtttacgccc attgaacaaa tgctaaaatt tcgcgccagc
    11161 cgccacgaag attttcctta tcaggagatc cttctgactc gtctttgcat gcacatgcaa
    11221 agcaagctgc tggagaaccg caataaaatg ctgaaggctc aggggattaa cgagacgttg
    11281 tttatggcgt tgattacgct ggagtctcag gaaaaccaca gtattcagcc ttctgaatta
    11341 agttgtgctc ttggatcatc ccgtaccaac gcgacgcgta ttgccgatga actggaaaaa
    11401 cgcggttgga tcgaacgtcg taaaagcgat aacgatcgcc gctgcctgca tctgcaatta
    11461 acggaaaaag gtcacgagtt tttgcgcgag gttttaccac cgcagcataa ctgcctgcat
    11521 caactctggt ccgcgctcag cacaacagaa aaagatcagc tcgagcaaat cacccgcaaa
    11581 ttgctctccc gtctcgacca gatggaacaa gacggtgtgg ttctcgaagc gatgagctaa
    11641 cgcgtcatct cgctcaaaaa tccagattta taaaagaaaa aatgaccggc cagcatcgca
    11701 acatgctggc ctttttggca agcaggtcgg ctcagccgat gagttaagaa gatcgtggag
    11761 aacaatatga gcgcaaatgc ggagactcaa accccgcagc aaccggtaaa gaagagcggc
    11821 aaacgtaagc gtctgctcct ccttctcacc ttgctcttta taattattgc cgtagcgata
    11881 gggatttatt ggtttttggt actgcgtcat ttcgaagaaa ccgatgacgc atacgtggca
    11941 gggaatcaga ttcaaattat gtctcaggtg tctggcagcg tgacgaaagt ctgggccgat
    12001 aacaccgatt ttgtaaaaga aggcgatgtt ctggtcactc tcgacccgac agatgctcgc
    12061 caggcgtttg aaaaagccaa aactgcactg gcttccagcg ttcgccaaac ccaccagctg
    12121 atgattaaca gcaaacagtt gcaggcgaat attgaggtgc aaaaaatcgc cctcgcgaaa
    12181 gcacaaagcg actacaaccg ccgtgtgccg ctgggcaatg ccaacctgat tggtcgcgaa
    12241 gagctgcaac acgcccgcga cgccgtcacc agtgcccagg cgcaactgga cgtcgctatt
    12301 caacaataca atgccaatca ggcgatgatt ctggggacta aactggaaga tcagcctgcc
    12361 gtgcaacagg ctgccaccga agtacgtaac gcctagctgg cgctggagcg tacccgtatt
    12421 gtcagtccga tgaccggtta tgtttcccgt cgtgccgtgc aaccgggtgc gcaaattagc
    12481 ccaacgacgc cgctgatggc ggtcgttcca gccaccaata tgtgggtgga tgccaacttt
    12541 aaagagacgc agattgccaa tatgcgcatc ggtcagccgg tcaccatcac caccgatatt
    12601 tacggcgatg atgtgaaata caccggtaaa gtggttggtc tggatatggg cacaggtagc
    12661 gcgttctcac tgcttccggc gcaaaatgcg accggtaact ggatcaaagt cgttcagcgt
    12721 ctacctgtgc gtatcgaact ggaccagaaa cagctggagc aatacccgct gcgcattggt
    12781 ttgtccacgc tggtgagcgt caataccact aaccgtgacg gtcaggtact ggcaaataaa
    12841 gtacgttcaa caccggtcgc ggtaagtacc gcgcgtgaaa tcagcctggc acctgtcaat
    12901 aaactgatcg acgatatcgt aaaagctaac gctggctaat ccagaggtgc gtgtgatgca
    12961 acagcaaaaa ccgctggaag gcgcgcaact ggtcattatg acgattgcgc tgtcactggc
    13021 gacattcatg caggtgctga actccaccat tgctaacgtg gcgatcccca ctatcgccgg
    13081 gaatctgggc tcatcgctca gccaaggaac gtgggtaatc acttctttcg gggtggcgaa
    13141 tgccatctcg atcccgctta ccggctggct ggcaaagcgc gtcggggaag tgaaactgtt
    13201 cctttggtcc accatcgcct ttgctattgc gtcttgggcg tgtggtgtct ccagcagcct
    13261 gaatatgctg atcttcttcc gcgtgattca ggggattgtc gccgggccgt tgatcccgct
    13321 ttcgcaaagt ctattgctga ataactaccc gccagccaaa cgctcgatcg cgctggcgtt
    13381 gtggtcgatg acggtgattg tcgcgccaat ttgcggcccg atcctcggcg gttatatcag
    13441 cgataattac cactggggct ggatattctt catcaacgtg ccgattggcg tggcggtggt
    13501 gttgatgaca ctgcaaaccc tgcgcggacg tgaaacccgc accgaacggc ggcggattga
    13561 tgccgtgggg ctggcactgc tggttattgg tatcggcagc ctgcagatta tgctcgaccg
    13621 cggtaaagag ctggactgtt tttcatcaca ggaaattatc attcttaccg tggtggcggt
    13681 ggtggctatc tgcttcctga ttgtctggga gctgaccgac gataacccga tagtcgatct
    13741 gtcgttgttt aagtcgcgca acttcaccat cggctgcttg tgtatcagcc tcgcgtatat
    13801 gctctacttc ggcgctattg ttctgctgcc gcagttgttg caggaggtct acggttacac
    13861 ggcgacctgg gcaggtttgg cctctgcgcc ggtagggatt attccggtga ttctgtcgcc
    13921 gattatcggc cgcttcgcgc ataaactgga tatgcggcgg ctggtaacct tcagctttat
    13981 tatgtatgcc gtctgcttct actggcgtgc ctataccttt gaaccaggta tggatttttg
    14041 cgcgtcggcc tggccgcagt ttatccaggg gtttgcggtg gtctgcttct ttatgccgct
    14101 gaccaccatt acgctgtctg gtttgccacc ggaacgactg gcggcggcat cgagcctctc
    14161 taactttacg cgaacgctgg cggggtctat cggcacgtcg ataaccacga ccatgtggac
    14221 caaccgcgag tcgatgcacc atgcgcagtt gactgagtcg gtaaacccgt tcaacccgaa
    14281 tgcccaggcg atgtacagtc aactggaagg gcttgggatg acgcaacagc aggcatcagg
    14341 ctggattgcc cagcagatca ccaatcaggg gctgattatt tccgccaatg agatcttctg
    14401 gatgtcagcc gggatattcc tcgtactgct ggggctggtg tggtttgcta aaccgccatt
    14461 tggcgcaggt ggcggcggag gcggtgcgca ctaagtacaa ctaagccagt tcatttgaac
    14521 tggctttttt caattaattg tgaagatagt ttactgacta gatgtgcagt tcctgcaact
    14581 tctctttcgg cagtgccagt tcttcgttgc tgttgatgcg tacctcacgt tccagaatgc
    14641 tacgcgcaat atcctgcgct tcctgcaacg agtgcatctg gtaagtgcca cactggtaga
    14701 cgttcagttc cgggatctga ttctgatcct gcactttcag cacgtcttcc attgccgctt
    14761 tccaggcatc agcaacacgc tgctcatctg gcgtaccaat cagactcata taaaaaccgg
    14821 tgcggcagcc cattggcgag atatcgataa tctctacacc attaccgtta agatggttac
    14881 gcataaaacc agcaaacagg tgctccaggg tatggatccc tctttctggc atcacttctt
    14941 tgttcggcac gcagaagcgc agatcgaaca cggtgattgc gtcgccatgc ggggtgttca
    15001 ttgttttcgc cacccgaact gcaggcgctt ccatccgggt atgatcgact gtgaagctat
    15061 ctaacaacgg catttagcca cctccggtaa tttttttaaa aattttctga actctttctt
    15121 cccaggcgag tctgagtata tgaaagacgc gcatttgtta tcatcatccc tgaattcaga
    15181 gatgaaattt tggccactcc cgagtggcct ttttcttttc tgtcaggcgt gtttttccag
    15241 ccacaccgca aacggttcgg tatcagcggt ttccatttcc tgctgacggc gttcagatgc
    15301 ctcgcgctcg gctacaaaat cctcttcgcg cagaatttcc agcggctctt cacgcagcag
    15361 attacggtag gcttctgcaa acgctttgcc tgttccgcca ataccagtat caatcataga
    15421 ccttaagata cgggcagaga aagtcagatc gggattatcg aagcaggcaa ccagttcatc
    15481 acacactttc tgatacgctt cgccgccgtt aatgctatcc agcgtttgcg cgacgcgttt
    15541 cagatcgcgg aacagatctt tacccacctg cagtaacggg aactgcgcgg tttcgcagcc
    15601 gatacccagc gtcaggcccg gtttgcgacc ttcaaggatc acccggttcc agttaacgcg
    15661 cgtacaggca agttcgctac tgctcatttc cggtgcatca gccagcgcac accagaccat
    15721 aaacaggtcg aggaatcgca cctgctgttc atctacacca atcggcgaga acgggttgat
    15781 gtccagcgaa cgcacttcaa tatattcaat gccgccacgt aacagcgcat cagaaggtga
    15841 ctcgccgctg cgggtaacgc gttttggacg aatcggcgca tacagttcgt tttcaatctg
    15901 cagcacgttg ctattgattt gcagcctctt accgtctttc tcaataccaa tcttcgcgta
    15961 ctcttccgat ggcgttttga ttgcctgttt aaggcccgct acgtactcgt aaagatcgtt
    16021 gaaggtaata ccaagattgc tttgcgattt attggtatag ccgagatcgc tcaaacgaag
    16081 agaggtcgca tacggcaggt aatacatacc gcactcggtt ttctcaaacg gcagcgacgt
    16141 tggttttcct tgcaggaaag aagaacaaat cgccggagat gcaccaaaca gataaggaat
    16201 aacccaaccg aaacgatagt aattgcggat aacgcggaaa tagcccgcag aaattttctc
    16261 tttggcatca gcgcccgaga tatcaccgca cttcgcttgc cagaatgcca ttggcaaaga
    16321 gaaattgtag tgcacgccgg aaatggtttg catcagcgcg ccgtagcgat ttttcagccc
    16381 ttcacgatac agcgttttaa agcgtccggt gttagaagtg ccgtactgtg ccagttcgat
    16441 gtcctgacct tctgcgatgt agcatggcat acttaacggc cacatccgct catcgcccat
    16501 attgcgcgcc gtataacgat gcagatcgcg cataaaggtc agcatatgtt caatatcacc
    16561 atccactggt gtaatgaatt ccagcaatgc ttccgcaaaa tcggtagtaa tccatttgtg
    16621 cgtcagtgcg gaacctaatg cttcaggatg acctgttgtt gccagtgtgc catcagcatt
    16681 aacacgcaaa gtttcgcgct ccagcccacg ctgtatcccc tttaacgcct gaggatgttt
    16741 ttccagccag gccagcgcct gtgatacgtc cgggatcaaa ttgacctccc gcctgtcaaa
    16801 atcgttttaa ttagcataac tgtaatggtg accatatgtg caggcctaca attagtgcca
    16861 ccacatcatg ccctgaacgg tcgctgctgc aactgcaaca tagcgtaacg ctttaccaag
    16921 gcacaaaaaa aagattactg gcccccagga gatgcgcatc catcccgcta acagacacag
    16981 taaatcgcca accacgggca tccagcttaa taatagcgtg actgcaccat agcgtttcag
    17041 ccagccggta gctttctctt gccagcgcga tgttttacgc aatggaaaga aacgcccaag
    17101 gataacgtta gttaaccctc caaggctatt acccattgtt gctgttaaaa ctaaaaccca
    17161 gggatgactg atcccggaaa gcaacattgc caccagcacg acttcggagt tgccgggtaa
    17221 tagtgtagcg ctgagaaaac tactggcaaa caacgagaaa agcgataacg cttcactcac
    17281 agcaagcgaa catccacagc gtccatgcct gctgcacggg ccgcctgaat accgaaatcg
    17341 gcatcttcaa agaccacaca ctgcgtcggt tgcacgccca tacgctgcgc gcacaacaaa
    17401 aatgtgtctg gcgcgggttt atggtgtttg acgtgatcag cagcgacgac ggcgtcaaaa
    17461 taacggcgta atcccaggtg cgccagcaat gcctcagcga tggcgctttc actccccgtt
    17521 cctacagcca ttgggcgacg accatgccaa cttttcacca cttcaacaag aggaagcggc
    17581 tcgacactat ccagcagcat acttcttact gcttctgttt tttcacgcgc taacgcatgc
    17641 gggtcgagat cggcctgatt cagctcaata attgcctgag caatacgcca ggtgggcgat
    17701 ccattaagcg caatcatcgc ctgaatatcg tactgaagac cgtagtgccc taatacttcg
    17761 cgccacgctt tacggtgcgt aggcgccgta tccaggattg tgccatccat atcaaaaatt
    17821 aaacctgcat aacgctcgta catggtcttc tcgcaagtcg aaaaatatga cgctacttta
    17881 gcgtaattgc ttgattttgt cgctgatggc gaaggagaag aaaattcagg aaatggataa
    17941 agtagttatt tagaaatgag atatttttga aggaaatttt ttggagaata tggtgcatcc
    18001 gggaggattc gaacctccga ccgctcggtt cgtagccgag tactctatcc agctgagcta
    18061 cggat
//
LOCUS       DAZOYF010000072        18006 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZOYF010000072 DAZOYF010000000
VERSION     DAZOYF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.072"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..2264
                     /locus_tag="ACHUOF_002333"
                     /pseudo
     CDS             1..2264
                     /locus_tag="ACHUOF_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2265..3914
                     /locus_tag="ACHUOF_002334"
     CDS             2265..3914
                     /locus_tag="ACHUOF_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR0611490.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3919..4695
                     /locus_tag="ACHUOF_002335"
     CDS             3919..4695
                     /locus_tag="ACHUOF_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR0611491.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4970..7819
                     /gene="rcsC"
                     /locus_tag="ACHUOF_002336"
     CDS             4970..7819
                     /gene="rcsC"
                     /locus_tag="ACHUOF_002336"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR0611492.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="ACHUOF_002337"
     CDS             complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="ACHUOF_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR0611493.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="ACHUOF_002338"
     CDS             complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="ACHUOF_002338"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR0611494.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            12096..13217
                     /gene="ompC"
                     /locus_tag="ACHUOF_002339"
     CDS             12096..13217
                     /gene="ompC"
                     /locus_tag="ACHUOF_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR0611495.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13329..14384
                     /gene="apbE"
                     /locus_tag="ACHUOF_002340"
     CDS             13329..14384
                     /gene="apbE"
                     /locus_tag="ACHUOF_002340"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR0611496.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14458..15522
                     /gene="ada"
                     /locus_tag="ACHUOF_002341"
     CDS             14458..15522
                     /gene="ada"
                     /locus_tag="ACHUOF_002341"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR0611497.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15522..16172
                     /gene="alkB"
                     /locus_tag="ACHUOF_002342"
     CDS             15522..16172
                     /gene="alkB"
                     /locus_tag="ACHUOF_002342"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR0611498.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            16248..17891
                     /gene="yojI"
                     /locus_tag="ACHUOF_002343"
     CDS             16248..17891
                     /gene="yojI"
                     /locus_tag="ACHUOF_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR0611499.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         4353 a         4382 c         4841 g         4430 t
ORIGIN      
        1 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
       61 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
      121 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct atgaccaggc
      181 gctctccagc gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat
      241 gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
      301 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
      361 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
      421 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgacaa
      481 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
      541 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
      601 ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
      661 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
      721 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
      781 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
      841 tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc tgatcccgct
      901 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
      961 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     1021 ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga
     1081 ctggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct
     1141 cgacagttat gccgagcagg cggataatat gccgttattg catcgggcgc tggtgctggc
     1201 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaaggggggg atgaagctat
     1261 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     1321 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     1381 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     1441 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     1501 tagcggtggt gacgctaccc agacggccgc tattttaatg gtttgaccgc tgagcaatcc
     1561 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     1621 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     1681 gactggcgtt gggttggtta gggtgtgccg gacattctct cttttggtga cgaattatcg
     1741 ccgcaaatgt gcaggtccgc tggcgtgaac cggcaaaaac ggctcaacaa agtaacattc
     1801 cggtgaccgt tgaacgccag ttgtatcggc ttatccccgg tgaagaagag atgagcttta
     1861 ctctgcagcc ggtgaccagc aatgagattg acagcgatgc gctgtatctc gatgaaatta
     1921 cgcttaccag cgagcaggat gcagttctgc gctatggtca ggtggaagta ccgctcccgc
     1981 cgggagctga cgttgagcgc acaacatggg gcatttcagt caataaaccc aacgccgcga
     2041 aacagcaggg gcaattgctg gaaaaagcgc gtaatgaaat gggcgaactg gcctatatgg
     2101 tgccggtgaa agaactgacg ggaacggtca ctttccgcca tttgctgcgc ttctcgcaaa
     2161 aagggcaatt cgttctgcct cctgctcgtt atgtgcgttc ctatgcacct gcgcagcaaa
     2221 gtgtcgcggc agggagtgaa tggaccggga tgcaggtgaa ataagtgaac tggcgcagaa
     2281 tcgtctggct attggcgttg gttactctgc caacgcttgc ggaggagacg ccgttacaac
     2341 tggtgctgcg cggtgcgcag cacgatcaac tgtatcagct gtcgtcatca ggcgtgacca
     2401 aagtctcagc attacctgac tcgctcacca cgccgttagg gagtttgtgg aaactgtatg
     2461 tttacgcctg gctggaggat acccatcaac cggagcagcc ctaccagtgt cgcggaaact
     2521 ccccggaaga ggtttattgt tgtcaggctg gggagagcat aacgcgcgat accgcgctgg
     2581 tacgttcctg tgggctctat tttgcgccgc agcggttgca tattggcgca gatgtgtggg
     2641 ggcaatactg gcaacagcgt caggcacctg cgtggttagc gtctcttaca acgctgaagc
     2701 cagaaacctc ggtaacggtt aaatcgctgc ttgattcatt agcgacgtta cctgcgcaaa
     2761 acaaagctca ggaagtctta ctggatgtgg tgctggacga ggcgaaaatc ggtgtcgcat
     2821 ccatgctcgg tagtcgggta cgggtgaaaa cctggagctg gtttgccgat gataaacagg
     2881 aaatccgtca gggtggtttt gctggctggt taaccgatgg tacaccgctg tgggttactg
     2941 gcagtggcac cagcaaaacg gtactaaccc gttatgcgac ggtgcttaat cgggttttgc
     3001 cagtgccgac gcaggttgcc agtgggcaat gtgtagaggt ggagctgttt gcccgctatc
     3061 cgctgaagaa aatcacagcg gaaaagagta cgacggcagt aaaacccagc gtacttaatg
     3121 ggcgctatcg tgtgacgttt actaatggta accacattac gtttgtcagc cacggtgaga
     3181 cgaccttact gagcgaaaag ggcaaactga aattgcagtc gcatctcgat cgtgaagagt
     3241 acgttgccag agttctggat cgcgaggcga aaagtacgcc acctgaggca gcgaaagcca
     3301 tgactgtggc gatacgtact tttttacagc aaaacgccaa tcgggaaggt gattgcctga
     3361 ccatccccga cagcagcgcc acgcagcgtg tttctgcttc gccagcgacc acgggggcgc
     3421 gaacgatgac cgcctggacg caggagctca tctatgcagg cgatccggtt cattatcatg
     3481 gcagtcgcgc taccgaaggg acactttcct ggcggcaagc cacggcccag gcggggcagg
     3541 gggagcgtta cgaccagata ctcgcgtttg cttatcccga caacagcctt agccgctggg
     3601 gagctccacg ctcaacctgc cagttattac ccaaagcgaa agcctggctg gcgaaaaaaa
     3661 tgccgcagtg gcggcgtata ttacaaggcg agacggggta caacgaacca gacgtgtttg
     3721 cggtctgtcg tttagtctca ggtttcccct ataccgatcg gcagcagaaa cggctgttta
     3781 ttcgtaactt ctttacgctt caggatcggc tggatttaac ccatgagtat ctgcacttgg
     3841 cctttgatgg ttatcccacc gggcttgatg agaactatat cgaaacgctg acccgacaat
     3901 tattaatgga ctaatgctat gcgaaagatt tttcttccgt tgttactggt ggcactttcg
     3961 cctgtcgctc acagtgaagg tgtgcaggaa gtcgagattg acgcaccgct ctctggctgg
     4021 catccggcag agggcgaaga tgccagtttt tcacaatcga tcaattaccc ggcgtcgtcc
     4081 gtcaacatgg cagatgatca aaatatctca gcgcagatcc gcggcaaaat aaagaattat
     4141 gctgcggcgg gtaaagttca gcagggccgg ctggtggtca acggtgccag tatgccgcag
     4201 agaattgaat ccgatggttc atttgcacgc ccttatattt tcactgaagg cagcaacagc
     4261 gtgcaggtca tcagcccgga tgggcaaagc cggcaaaaaa tgcagtttta ctcaacgccg
     4321 ggtacaggaa ctattcgtgc acgtttacgg ctggttctct cgtgggatac ggacaatacc
     4381 gatctcgatc tccatgttgt cacgcccgat ggcgaacacg cctggtacgg taacaccgtg
     4441 ctgaaaaaca gtggtgcact ggatatggat gtcacgacgg ggtacggacc cgagattttc
     4501 gccatgccag caccagttca cgggcgctat caggtgtata tcaactacta tggtgggcgc
     4561 agcgaaacgg aattgacgac cgcccaactg acgctgatca ccgatgaagg atcggtcaat
     4621 gagaaacagg aaacatttat tgtaccgatg cgtaatgctg gcgagctgac gctggtgaaa
     4681 agttttgact ggtgatccat cccttcacac aacctggctc tctctttttt cagataagac
     4741 tgctgggcga tctgccgacg ccaggatata accattaaca agcgtcggca gattggtcat
     4801 ttcttatttt tcgtactgag aactgtccat ctactccggc tttcctggtt aaggcaatgg
     4861 tttctaagcg atacgcatta ttgcaatggt gacagaacat tgcggtaaca cgcttttacc
     4921 gctaccttaa ccacactcca tcggtcacct gaggcggagc ttctcccctt tgaaatacct
     4981 tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg ttcagagcat tggcgttagt
     5041 gctctggctg ttgattgctt tttcatccgt tttttacatc gttaatgcgt tacatcagcg
     5101 agaatcggaa attcgtcagg aatttaatct gagttccgat caggctcagc gctttattca
     5161 acgcacctct gatgtgatga aagagcttaa gtacatcgcc gaaaatcgct tatcggcaga
     5221 aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg gatgtgcctg cgtttgaacc
     5281 gctgtttgcc gactccgatt gttccgcaat gagtaacacc tggcgaggtt ctctggagtc
     5341 attggcgtgg tttatgcgct actggcgcga taatttttct gcggcttacg atctcaaccg
     5401 ggtattttta atcggcagtg ataacctctg catggccaat ttcggtctgc gtgatatgcc
     5461 agtggaacgc gataccgcgt tgaaagcgtt gcatgaacgc atcaataaat atcgaaatgc
     5521 accacaagat gatagcggca gtaacctcta ctggatcagc gaaggtccgc gcccttgcgt
     5581 cgggtatttt tacgcgttga cgccagttta tctggcgaac cggttgcagg cgcttttggg
     5641 tgtcgagcag accatccgga tggagaactt tttcttaccg ggtacgttgc cgatgggggt
     5701 taccattctt gatgaaaatg gtcataccct gatttcgctt accggaccag aaagtaaaat
     5761 taagggcgat cctcgctgga tgcaggaacg ctcctggttt ggctatacgg aagggttccg
     5821 ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta agcatcgtgt attcggtgcc
     5881 ggttgataag gtgctggaac gcattcgcat gttgatcctt aacgcaattt tgctgaatgt
     5941 gcttgccgga gctgcattgt ttactctcgc gcggatgtac gagcgacgta ttttcattcc
     6001 ggcggaaagc gacgccctgc gactggaaga acatgagcag ttcaatcgca agattgtcgc
     6061 ctccgcgcca gtgggtatct gcattttgcg taccgctgat ggcgtcaata ttttaagtaa
     6121 cgaactggcg catacctatc tcaatatgct tacgcatgag gaccgccaac gactgacgca
     6181 aattatctgt gggcagcagg tcaattttgt tgatgtcctg accagcaaca ataccaatct
     6241 gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac gtggccattt gtgtgctggt
     6301 ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag gagatggcac aagcagcgga
     6361 acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc agtcatgagc tgcgaacgcc
     6421 gctgtatggc attatcggta acctggatct gttgcaaacc aaagagttac cgaaaggcgt
     6481 cgatcgtctg gtgacggcaa tgaacaactc ttccagcctg ttgttgaaaa ttatcagcga
     6541 tattctcgat ttctcgaaga ttgaatcgga acagttgaag atcgaaccgc gtgagttttc
     6601 accgcgtgaa gtgatgaacc acatcaccgc caactattta ccgctggtgg tacgcaagca
     6661 gttaggcttg tactgcttta ttgaaccgga tgtgccagtg gccttaaatg gcgacccgat
     6721 gcgtttacag caggtcatct ccaacctgtt gagtaacgcc ataaaattca ccgataccgg
     6781 ctgtatagtt ttgcatgttc gcgctgatgg cgattatctc tctatccgtg ttcgcgatac
     6841 cggcgtgggg attccggcga aagaagtggt gcgcttgttt gatcccttct tccaggttgg
     6901 aacgggcgta cagcgaaact tccaggggac cggtctgggt ctggcgattt gtgaaaaact
     6961 gatcagcatg atggacggcg atatctcggt agattcagaa ccgggaatgg gcagccagtt
     7021 taccgtgcgt attccgttgt acggcgctca gtacccgcag aaaaaaggcg tggaagggtt
     7081 gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg ctctgtcagt tcctggaaac
     7141 cagtttgcag cgcagcggca tcgtcgttac aacatacgaa gggcaggaac cgactcccga
     7201 agatgtgtta atcactgacg aggtagtgag taaaaaatgg cagggcagag cggtagtgac
     7261 cttctgtcgt cgccatattg gtattccgct ggagaaagcg ccaggggagt gggtacacag
     7321 tgtggctgct ccgcatgagc taccggcatt gttggcgcgt atttatttga tcgagatgga
     7381 gagcgacgat cctgctaacg ctctgccgtc gacggacaaa gcggtcagcg ataacgacga
     7441 tatgatgatt ctggtcgtgg atgatcatcc gattaaccga cgtctgctgg cagatcagtt
     7501 gggatcgttg ggctatcaat gtaaaaccgc gaatgatggc gtcgatgcgc ttaatgtact
     7561 tagtaagaat catattgata tcgtgcttag cgacgtcaac atgccaaata tggatggtta
     7621 ccgcttgacg caacgcattc gtcagttggg actgacgttg ccggtaatcg gagtaactgc
     7681 taatgcgttg gctgaagaga agcagcggtg tctggagtcc ggtatggaca gctgcctctc
     7741 gaagccggta acgctggatg tgataaaaca gacgctgacg gtatatgccg agagggtcag
     7801 gaaatcgcgg gattcgtagg acggataagg cgtttacgcc gcatccggca ttcagtgcaa
     7861 atgccagata agacactgac gcgtcttatc tggcctactt taatgtaatc tgttgaaata
     7921 atgggaatcg taggccggat aaggcgttta cgccgcatcc ggcaatcggt gcaaatgcca
     7981 gataagacac taacgcgtct tatctggcct acagatgatt agtctttatc tgccggactt
     8041 aaggtcacag aagagagata attcagcagg gcgatatcgt tctcgacgcc cagcttcatc
     8101 atcgcagatt tcttctggct actgatggtt ttaatactgc ggttcagctt tttagcgatc
     8161 tcggtcacta ggaagccttc cgcaaacagg cgcagaactt cactctcttt tggcgagaga
     8221 cgcttgtcac cgtaaccacc agcactgatt ttttccaaca ggcgagaaac gctttccggg
     8281 gtaaatttct tccctttctg cagcgcggcg agagctttcg gcagatcggt cggtgcacct
     8341 tgtttcagca cgatcccttc gatatccaga tccaataccg cactaagaat cgccgggttg
     8401 ttgttcatag tcagaacaat gatcgacagg cttgggaaat ggcgcttgat gtacttgatt
     8461 aaggtaatgc catcgccgta cttatcgcca ggcatggaga gatcggtaat caacacatgc
     8521 gcatccagtt tcggcaggtt gttgatcagt gctgtagagt cttcaaattc gccgacaaca
     8581 ttcacccact caatttgctc aagtgatttg cgaataccga acaagactat cggatggtca
     8641 tcggcaataa ttacgttcat attgttcatg taataggcta ccttgctaca gcaagctctt
     8701 gacataactg tcaatgtcgc tgatgtattt ttctattcct ggaacatcct tctcacgaat
     8761 cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca tggcaaatac
     8821 gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggttg ctgcttcagt
     8881 atacagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat agccgctggc
     8941 atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt cctgtgccag
     9001 ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat tgaagttgac
     9061 gcacaattga ccaggcccaa tttctcgtac gccagactca tcatcgctta aaagcaagcc
     9121 agaggcagta agattagacg gattatccgt taaaaagata tcataatctt gactaattaa
     9181 tctttcatcg ggtgtgatac aggttgcacc ccaattttct aacaggcgag tgacaatatt
     9241 ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta aacgctcttc
     9301 ttcctcttca acttccgggt cagcggcgag cattttgata tgcacagagt agcgtgtacc
     9361 aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgtgcca gttgatcgct
     9421 cagccagaat gccagcgggt ccgcctttcc ataacgatcg ttttgggtct ggttgataaa
     9481 cgggaagtgc aaattatcca tttcatgaat acttacgcct tctcccgtgt ccagaatgcg
     9541 gaatgtcagg cggtcttcag aggactcatc ctgatcaacc tcaagggtga tttttcccaa
     9601 ttgcgttgag gtcacggcat attgcatcag tagcagcaaa atacgtcgta aggcatcgcg
     9661 atcgccgcgg cgcatatcgt gtgctttcag atgattgtta atcagcagtt gcagaccttt
     9721 acgcttgatg gcaggcaaca ctgaaggcac aacttcatca attaaatcct gcacggagaa
     9781 cagcaccgtc tcacttttcc agctatcatc cgcaagcatg ttcgctaact gtatttcttc
     9841 gaccagccgc accagcacat ctgcctgatt cgccagttgt ttgctttccg gggcgttgag
     9901 tttagccgca ctctccgcca gggactgtgc gggttctttc agcgcatcgc caatgttttt
     9961 cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct gcttgagttt
    10021 cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg tgcgcggcgc
    10081 gacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg cctgaataat
    10141 cccctgatgc tgttccgcca tggtggtgat gttttgcaga ttcagatgcg gcagcaaatg
    10201 atcggcaatt ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa ccagcaggcc
    10261 gagcggcaac agtgagacta tctcttcatt gattgcccgt aaaatgcgca attcgttatt
    10321 gaccgccgtg ctggggacgc tttctgtact gcggctggag aaatggcgga atgtggtata
    10381 gccaaataac gccagcgcca gcaaaccgat gttcagcagc agtggcagca gaatgttttg
    10441 caacgtatcc agcaataagg tgccataagg aacctgccag accaggcgca tatcggtaga
    10501 gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga cgctatccgt
    10561 cccttctttc tcattatcat tgtttcccgt tgccgtcgcg tctggctcaa ggcggaaact
    10621 gtccagcggc atacccggtg ggatcaaatc attaatcggc agatcaaaag ccacgaccgt
    10681 tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac cgttctgcca
    10741 ggctaggcgg cgcatgttag aaaaactttc gcgttcatcg agggcgttgg cctgttgtaa
    10801 catttctgca cgacgtgaat caacaatgtc actgacggtc gattctttaa atccggaggt
    10861 gagatcttta aggggtaggg ttgagatcag caccagactg ttatcctgac cattcaggta
    10921 atacatcgac cacggtacat tttctgcacc ccacaatgta tccagataag tggacatccg
    10981 ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag attaacgctt ccgttttgcg
    11041 gcgtggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta aaccttcgcc
    11101 ggaggagggg gaggtcgtcg cggcaatgtt gtcgtagatc tgcctggtca cgtaacgcca
    11161 ggtatcgacg cgcttttgga ttgcgtgggt aatgtcgaca atctggtaac ttttatcttt
    11221 cagccaagcg ttaacggcac tttgtaccat tacccccatc gtcaccagta acacaatgat
    11281 caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg tcgtggccgt
    11341 tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag tgatagggca
    11401 gtataaaggg tacagtgtga aattccagac tcttacctga ttgatgattc tctattctcc
    11461 cgtgatattt atcatcctgt atggggggaa atagatgtac aagcgccatt ttgctgtaca
    11521 aataacctac aaaaagccca acgaaattaa ttgcaaataa acaaagattg ctggaaatta
    11581 tgctgatgtt aattatttgt gaaatagtta acaagcgtta tagtttttct gtggtagcac
    11641 agaataatga aaagtgtgta aagaagggta aaaaaaccga atgcgaggca tccggttgaa
    11701 ataggggtaa acagacattc agaaatgaat gacggtaata aataaagtta atgatgatag
    11761 cgggagttat tctagttgcg agtgaaggtt ttgttttgac attcagtgct gtcaaatact
    11821 taagaataag ttattgattt taaccttgaa ttattattgc ttgatgttag gtgcttattt
    11881 cgccattccg caataatctt aaaaagttcc cttgtattta cattttgaaa catctatagc
    11941 gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt
    12001 tggggagaat ggacttgccg actgattaat gagggttaat cagtatgcag tggcataaaa
    12061 aagcaaataa aggcatataa cagagggtta ataacatgaa agttaaagta ctgtccctcc
    12121 tggtcccagc tctgctggta gcaggcgcag caaacgctgc tgaagtttac aacaaagacg
    12181 gcaacaaatt agatctgtac ggtaaagtag acggcctgca ctatttctct gacgacaagt
    12241 ctgtagatgg cgaccagacc tacatgcgtc ttggcttcaa aggtgaaact caggttactg
    12301 accagctgac cggttacggc cagtgggaat atcagatcca gggcaacagc gctgaaaacg
    12361 aaaacaactc ctggacccgt gtggcattcg caggtctgaa attccaggat gtaggttctt
    12421 tcgactacgg tcgtaactac ggcgtagttt acgacgtaac ttcctggacc gacgtactgc
    12481 cagaattcgg tggtgacact tacggttctg acaacttcat gcagcagcgt ggtaacggct
    12541 tcgcaaccta ccgtagcacc gacttctttg gtctggttga cggtctgaac ttcgctgttc
    12601 agtatcaggg caaaaacggt agcccggaag gtgaaggcat gaccaacaac ggtcgtgaag
    12661 ctctgcgtca gaacggtgac ggcgttggcg gttctattac ttatgattac gaaggcttcg
    12721 gtatcggtgc tgcagtttcc agctccaaac gtactgatga ccagaacttc ggtctgaacc
    12781 gttatgatga gcgttacatc ggtaatggcg accgcgctga aacctacacc ggtggtctga
    12841 aatacgacgc gaacaacatc tacctagctg ctcagtacac ccagacctac aacgcaactc
    12901 gcgtaggcaa cttgggttgg gcgaacaaag cacagaactt cgaagctgtt gctcagtacc
    12961 agttcgactt cggtctgcgt ccgtccctgg cttacctgca gtctaaaggt aaaaacctgg
    13021 gtgtcatcaa tggtcgtaac tacgacgacg aagatatcct gaaatatgtt gatgttggtg
    13081 cgacctacta cttcaacaaa aacatgtcca cctacgttga ctacaaaatc aacctgctgg
    13141 acgacaacca gttcactcgt gacgctggca tcaacactga taacatcgta gctctgggtc
    13201 tggtttacca gttctaatct cgatggatat cgaacaaagg gcctgcgggc ccttttttca
    13261 ttgttttcag cgtacaaaca cagttttttg gtgtactctt gcgaccgttc gcatgaggat
    13321 aatcacgtat ggaaataagc tttacccgcg tggcactgct ggttgccgcg ctcttctttg
    13381 ttggttgcga tcaaaaacca caacccgcca aaacccacgc tactgaagtt accgttcttg
    13441 aaggcaaaac catgggtacc ttctggcgtg ccagcatccc gggcattgat gccaaacgca
    13501 gtgccgaact taaagaaaag attcagaccc agctggacgc cgacgatcag ctgctttcga
    13561 cctataaaaa agattccgcg ctgatgcgct ttaacgactc gcaaagtttg tcgccgtggc
    13621 cggtaagtga agcgatggcc gatatcgtca ccacctcgct gcgcattggg gcaaggaccg
    13681 atggtgcgat ggatattacc gtcgggccgc tggtgaatct gtggggcttt ggcccggaac
    13741 aacagccggt tcaaattccg agccaggaac agatcgatgc gatgaaagcc aaaaccggct
    13801 tacagcacct gacggtcatc aatcagtcgc atcagcaata tctgcaaaaa gacctgccgg
    13861 atttatatgt cgatctctcc accgtcggtg aaggttatgc ggcggatcac ctggcacgct
    13921 tgatggagca ggaagggatt tcccgctatc tggtgtcggt gggcggcgcg ctgaacagcc
    13981 gtggtatgaa cggtgaaggc cagccgtggc gggtagcgat tcaaaaacca accgataaag
    14041 aaaacgcggt tcaggctgtg gtggatatca acggtcatgg gattagcacc tctggcagct
    14101 accgtaacta ttacgaactg gacggcaaac gtctttccca tgttatcgat ccgcaaaccg
    14161 ggcgtcccat cgaacacaat ctggtatccg tgacggtgat tgctccgacg gcgctggaag
    14221 ccgatgcctg ggatactggc ttgatggtac tcgggccgga gaaagccaaa gaggttgttc
    14281 gccgggaagg gctggcggtt tatatgatca ccaaagaagg cgatagcttt aaaacctgga
    14341 tgtcaccaca gtttaaaagc ttccttgtca gcgaaaaaaa ttaaagcgca agattgttgg
    14401 tttttgcgtg atggtgaccg ggcagactaa aggctatcct taaccaggga gctgattatg
    14461 aaaaacgcca catgcttaac tgacgatcaa cgctggcaat ctgtcttagc ccgcgacccg
    14521 aatgccgacg gcgaattcgt tttcgccgtg cgcaccacgg gtatcttttg ccgtccttct
    14581 tgccgcgcca gacatgcctt gcgggaaaat gtctccttct acgcaaatgc cagcgaggca
    14641 ctcgccgccg gatttcgccc ctgcaaacgt tgtcagccag acaaagccaa tccccggcaa
    14701 catcggttgg ataaaatcac ccacgcgtgt cgactgctgg aacaggaaac gcctgtaacg
    14761 ctggaagcct tagccgacca ggtggcgatg agtccatttc atctacatcg gttgtttaaa
    14821 gctactaccg gaatgacgcc taaagcctgg caacaggcct ggcgcgctcg ccgtttgcgc
    14881 gaatcgctgg cgaaagggga gagcgtgacg acgtctattc ttaacgccgg attccccgac
    14941 agcagcagtt actaccgcaa agccgatgaa acgctgggca tgacggctaa acaattccgt
    15001 cacggtggcg aaaatctggc ggtgcgttac gcgctggctg attgtgagct gggtcgttgc
    15061 ctggtggcag aaagcgagcg ggggatttgc gcgatattgc tgggcgatga tgacgcgaca
    15121 ttaatcagcg agttgcagca gatgtttccc gctgccgaca acgcgcctgc cgatctgacg
    15181 tttcagcaac atgtgcgtga agtgatcgcc agcctcaatc aacgcgatac gccgctgacg
    15241 ttaccgctgg atattcgcgg cactgctttt cagcaacaag tctggcaggc actgcgcacg
    15301 ataccttgtg gtgaaaccgt cagttatcag caactggcta acgccatcgg caaaccgaaa
    15361 gcggtacggg ccgttgccag cgcctgtgcc gccaacaagc tggctatcgt aataccctgt
    15421 catcgggtgg tccgtggtga tggcacactt tccggttacc gctggggcgt gtcgcgtaaa
    15481 gcgcaactgc tgcgccgcga agctgaaaat gaggagaggt aatgttggat ctgtttgccg
    15541 atgctgaacc gtggcaagag ccactggcgg ctggtgcggt aattttacgg cgttttgctt
    15601 ttaatgctgc ggagcaacta atccgcgata ttaatgacgt tgccagccag tcgccgtttc
    15661 gccagatggt cgctccaggc ggatatacca tgtcggtggc gatgaccaac tgtgggcatc
    15721 tgggctggac gacccatcgg caaggttatc tctattcgcc cattgatccg caaacaaata
    15781 aaccgtggcc cgctatgcca caaagctttc atgatttatg tcaacgtgcg gctacggcgg
    15841 cgggctatcc agatttccag ccagatgctt gtcttatcaa ccgctacgct cctggcgcga
    15901 aactgtcgct gcatcaggat aaagacgaac cggatctgcg cgcgccaatt gtttctgttt
    15961 ctctgggctt acccgcgatt tttcaatttg gtggcctgaa acgtaatgat ccgctcaaac
    16021 gtttgttgtt ggaacatggc gatgtggtgg tatggggcgg tgaatcgcgg ctgttttatc
    16081 acggtattca accgttgaaa gcggggtttc atccactcac caccgactgc cgctacaacc
    16141 tgacattccg tcaggcaggt aaaaaagaat aaaaataaga attattattg ctgtgcgcgc
    16201 gaagatgttt aaactgcggg ctgtaattca ttgtccgggt tttctgcatg gaacttcttg
    16261 tacttgtctg gcggcagtat cgctggccat ttatcagtgt gatggcgcta agcctcgcca
    16321 gtgcggcatt aggcattggc ttaattgctt ttatcaatca gcgccttatc gaaacggcgg
    16381 ataccagtct gctggtgtta ccggagtttc tgggattatt gctgctgttg atggcagtca
    16441 ctctcggatc gcaactggcg ctcaccactt tggggcatca cttcgtttac cgactgcgta
    16501 gtgaatttat caagcggatt ctggataccc acgtcgagcg cattgaacaa ctcggtagcg
    16561 cctcgttgct ggcgggatta accagcgatg tgcgcaatat caccattgct tttgtgcgtc
    16621 tgccggaact ggggcagggg atcattctca ctatcggttc atcggcgtat ctgtggatgc
    16681 tgtcgggcaa aatgttgctg gtaacggcta tctggatggc gatcaccatc tggggcggtt
    16741 ttgtgctggt ggcgcgggtg tacaaacata tggcgaccct acgtgaaacc gaagacaagc
    16801 tgtacacgga ttttcaaact gtacttgaag ggcgcaaaga gctgactctg aaccgggaac
    16861 gcgccgagta tgtgtttaac aacctctaca tccccgatgc gcaagagtat cgccatcata
    16921 ttattcgcgc agacaccttc catcttagtg ccgtgaactg gtcaaacatc atgatgctgg
    16981 gcgctatcgg cctggtgttc tggatggcga acagtctcgg ttgggctgat accaacgttg
    17041 ctgcgaccta ttcgttgacg ctgttattcc tgcgtacgcc gctgctttcg gcggttggcg
    17101 cattgccgac gctgctgacg gcgcaggtgg cgtttaacaa gctgaacaaa ttcgcgctcg
    17161 cgcctttcaa agctgagttt ccgcgcccgc aggcgtttcc caactggcaa acgctggagc
    17221 tgcgtaacgt gacgtttgct tatcaggata acgcgttttc cgttggtccg attaatctca
    17281 ccatcaaacg tggcgagctg ctgtttctga ttggcggtaa cggtagcgga aaatcgacgc
    17341 tggcgatgtt gttgacgggc ttgtatcagc cacaaagcgg cgaaatcttg ctggatggca
    17401 aacctgtcag cggcgaacaa ccggaagatt atcgcaaact gttttcggca gtgtttaccg
    17461 atgtctggct gtttgatcaa ctgctggggc cggaggggca acccgctaac ccgcaactgg
    17521 ttgagaagtg gctggcgcag ctgaaaatgg ctcataagct tgagttaagc aacgggcgta
    17581 ttgttaacct gaagttatca aaagggcaga aaaaacgcgt ggcgctgttg ctggcgctgg
    17641 cagaagaacg cgatattatc ctgctggatg aatgggcggt ggatcaggat ccacacttcc
    17701 gtcgtgagtt ttatcaggtg ttgctgccgc tgatgcagga gatgggtaaa actattttcg
    17761 ctatcagtca tgatgatcat tactttatcc acgccgaccg cctgctggaa atgcgcaatg
    17821 ggcaacttag cgagctgacg ggcgaagagc gcgatgccgc ttcgcgtgat gccgttgccc
    17881 ggacggcata atattacgac gctgacctgg cggactgctg ccgtcaggtc aatatgctaa
    17941 aaatcccttc atttctaata accctataat taatcaacga aattataatg tttctaaaat
    18001 tagaat
//
LOCUS       DAZOYF010000073        17888 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZOYF010000073 DAZOYF010000000
VERSION     DAZOYF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.073"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            76..600
                     /locus_tag="ACHUOF_002344"
     CDS             76..600
                     /locus_tag="ACHUOF_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR0611500.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            681..1355
                     /locus_tag="ACHUOF_002345"
     CDS             681..1355
                     /locus_tag="ACHUOF_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0611501.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1371..3833
                     /locus_tag="ACHUOF_002346"
     CDS             1371..3833
                     /locus_tag="ACHUOF_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR0611502.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            3849..4882
                     /locus_tag="ACHUOF_002347"
                     /pseudo
     CDS             3849..4882
                     /locus_tag="ACHUOF_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4964..5302)
                     /gene="rcnB"
                     /locus_tag="ACHUOF_002348"
     CDS             complement(4964..5302)
                     /gene="rcnB"
                     /locus_tag="ACHUOF_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR0611503.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5521..6351)
                     /gene="rcnA"
                     /locus_tag="ACHUOF_002349"
                     /pseudo
     CDS             complement(5521..6351)
                     /gene="rcnA"
                     /locus_tag="ACHUOF_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6473..6745
                     /gene="rcnR"
                     /locus_tag="ACHUOF_002350"
     CDS             6473..6745
                     /gene="rcnR"
                     /locus_tag="ACHUOF_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR0611504.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6837..6933
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6968..7756
                     /gene="thiM"
                     /locus_tag="ACHUOF_002351"
     CDS             6968..7756
                     /gene="thiM"
                     /locus_tag="ACHUOF_002351"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR0611505.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7753..8553
                     /gene="thiD"
                     /locus_tag="ACHUOF_002352"
     CDS             7753..8553
                     /gene="thiD"
                     /locus_tag="ACHUOF_002352"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR0611506.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8618..9435
                     /locus_tag="ACHUOF_002353"
                     /pseudo
     CDS             8618..9435
                     /locus_tag="ACHUOF_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            9487..10233
                     /locus_tag="ACHUOF_002354"
     CDS             9487..10233
                     /locus_tag="ACHUOF_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0611507.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10207..11172)
                     /locus_tag="ACHUOF_002355"
     CDS             complement(10207..11172)
                     /locus_tag="ACHUOF_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR0611508.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11169..12172)
                     /locus_tag="ACHUOF_002356"
                     /pseudo
     CDS             complement(11169..12172)
                     /locus_tag="ACHUOF_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12212..13264
                     /gene="fbaB"
                     /locus_tag="ACHUOF_002357"
     CDS             12212..13264
                     /gene="fbaB"
                     /locus_tag="ACHUOF_002357"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR0611509.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13572..14426
                     /gene="gatY"
                     /locus_tag="ACHUOF_002358"
     CDS             13572..14426
                     /gene="gatY"
                     /locus_tag="ACHUOF_002358"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR0611510.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14455..15717
                     /gene="gatZ"
                     /locus_tag="ACHUOF_002359"
     CDS             14455..15717
                     /gene="gatZ"
                     /locus_tag="ACHUOF_002359"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFR0611511.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15727..16179
                     /gene="gatA"
                     /locus_tag="ACHUOF_002360"
     CDS             15727..16179
                     /gene="gatA"
                     /locus_tag="ACHUOF_002360"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR0611512.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16210..16494
                     /gene="gatB"
                     /locus_tag="ACHUOF_002361"
     CDS             16210..16494
                     /gene="gatB"
                     /locus_tag="ACHUOF_002361"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR0611513.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16498..17853
                     /locus_tag="ACHUOF_002362"
     CDS             16498..17853
                     /locus_tag="ACHUOF_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR0611514.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4663 a         4094 c         4553 g         4578 t
ORIGIN      
        1 tttgatgtga gaaatttgtt tccaagagat acagactctt aacaaccaaa aataaatttt
       61 attaaggaac atattatgaa acgttcaatt attgctgccg ctgtcttttc ttcttttttt
      121 atgagcgctg gagtatttgc tgcagacgtt gataccggaa cattaaccat taaaggaaat
      181 attgcagaat ctccgtgtaa attcgaagcg ggtggtgatt cagtaagtat taatatgccg
      241 actgtaccaa ccactgtctt tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc
      301 ggtgtaacca gcagcatgtt aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc
      361 tcgttgatca caaacgacaa aataaccggt aacgataagg cgatagccag tagcaacgat
      421 accgtaggtg ataacagcga tgtcctagat gtttctgcac cttttaacat tgagagttat
      481 aaaacagcgg aaggtcaata tgctattccg tttaaagcaa aatacctgaa actgacagat
      541 aactcagtgc aatcaggtga tgtgttatct tctctggtta tgcgtgtggc gcaggattaa
      601 taaataaaat tgcaagtttc tccaggtaat aaacatggcc gctatcccca tggcggcttt
      661 ttaatttaag aggcattaag atgaaaggat tattatcttt actcattttt tctatggtcc
      721 ttcctgcaca tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata
      781 aagaagttat ggtgcagttg atgaaccagg gaaatcgttc ttcgctgctg caggcgtgga
      841 ttgatgatgg cgatacgtcg ttaccaccag aaaaaattca ggttcctttc atgttaacgc
      901 caccagtggc aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga
      961 ataaactgcc cactaataaa gaaagcattt tttatctgaa tgttctgtat attccaccaa
     1021 atagtccaga gcaagaaggc aagaatgcac tgaagtttgc gatgcaaaac agaattaagt
     1081 tgttttaccg gccagcgggt attgctccgg taaataaagc gacctttaaa aagttactgg
     1141 taaatcgcag tggcaatgga ttggtgataa aaaatgactc agctaattgg gtgacgattt
     1201 cggatgtcaa agctaataat gttaaagtca attatgaaac tattatgatt gcccccttag
     1261 aaagtcagag tgttaatatc aaaagtaata atgcaaataa ctggtatctg accattatca
     1321 atgaccacgg caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa
     1381 tgaccccact tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg
     1441 aagaaacctt tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata
     1501 ttcgtcttga tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata
     1561 agcaatggcg cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat
     1621 caagagaagt tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat
     1681 gtttaacatt tgagcaactt gttcagggtg ggagctatag ctgggatatc ggggtttttc
     1741 gtctcgattt cagtgtcccg caggcttggg tggaagaact ggaaagtggc tatgttccac
     1801 cggaaaactg ggagcggggt attaatgcgt tttatacttc ttattatgtg agtcagtatt
     1861 acagcgacta taaagcgtcg ggtaacaaca agagtacata tgtacgtttt aacagcgggt
     1921 taaatttact ggagtggcaa ctgcattctg atgccagttt cagtaaaaca aataacaatc
     1981 caggggtgtg gaaaagcaat accctgtatc tggaacgtgg atttgcccaa tttctcggca
     2041 cgcttcgcgt gggtgatatg tacacatcaa gcgatatttt tgattctgtt cgcttcagcg
     2101 gtgtgcggtt gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat tttacgccac
     2161 gggtgcaggg gattgctcag agtaacgcgc tggtaactat tgaacagaat ggttttgtgg
     2221 tttatcagaa agaggttcct cctggcccgt ttgcgattac agatttgcag ttggccggtg
     2281 gtggagcaga tcttgatgtt agcgtgaaag aggcggacgg ctcggtaacc acctatctgg
     2341 tgccttatgc agcggtgcca aatatgctgc aacccggcgt gtcgaaatat gattttgcgg
     2401 cgggggcgag caagcaaagt gattttgtcc aggcaggtta tcagtatggt tttaataact
     2461 tattgacgct gtatggcggc acgatggttg ctaataatta ctatgcgttt accctcggaa
     2521 cgggttggaa cacgcgcatt ggcgcaattt ccgtcgatgc cacgaagtcg catagtaaac
     2581 aagacaacgg cgatgtgttt gacgggcaaa gttatcaaat tgcctacaac aaatttgtga
     2641 gccaaacgtc gacgcgtttt ggtctggcgg cctggcgtta ttcgtcgcgt gattaccgga
     2701 catttaacga tcacgtttgg gcaaacaata aagataatta tcgccgtgat gaaaacgata
     2761 tctatgacat tgccgattat taccagaacg attttggccg taaaaatagc ttttctgcca
     2821 atatgagcca gtcattgcca gaaggctggg gttctgtgtc cttaagtacg ttatggcgag
     2881 attactgggg gcgtagcggc agcagtaagg attatcagtt gagttattcc aataactggc
     2941 ggcggataag ctataccctc gcggcaagcc aggcgtatgg cgagaatcat catgaagaga
     3001 aacgttttaa tatttttata tcaattccct gtgattgggg tgatgacgtt acgacgcctc
     3061 gtcggcaaat atatatgtct aactcaacga cgtttgatga tcagggtttt gcctcaaata
     3121 atacgggatt atcaggaaca gtagggagtc gggatcagtt caattatggt gtcaacctga
     3181 gtcatcaaca tcagggaaat gaaacgacag ctggggcgaa tttgacctgg aacgcgccgg
     3241 ttgcgacagt gaatggcagt tatagtcagt cgagtactta tcgacagact ggagccagtg
     3301 tttcaggggg cattgtcgcc tggtcgggtg gcgttaatct ggcgaaccgt ctttccgaaa
     3361 cgtttgctgt gatgaatgcg ccaggaatta aagatgctta tgtcaatggg caaaaatatc
     3421 gcacaacaaa ccgtaatgga gtggtggtat acgacggaat gacaccttat cgggaaaatc
     3481 acctgatgct ggatgtgtcg caaagcgata gcgaagcaga attacgtggc aaccggaaaa
     3541 ttgccgcccc ttatcgcggc gcggttgtac tggttaattt tgataccgat cagcgcaagc
     3601 cctggtttat aaaagcgtta agagcggatg gtcaaccatt aacgtttggt tatgaagtca
     3661 atgatatcca tggtcataat attggcgttg tcggccaggg aagccagtta tttattcgca
     3721 ccaatgaaat accgccatcg gttaatgtag caattgataa gcaacaagga ctttcatgca
     3781 caatcacctt cggtaaagag attgatgaaa gtagaaatta tatttgccag taatagtcag
     3841 gtggttctat ggaaattcgc ataatgctat ttatattaat gatgatggtt atgcctgtga
     3901 gctatgcggc atgttatagt gagttatctg ttcagcacaa tttggttgtt caggggaatt
     3961 ttgcacttac tcaaacacaa atagcgacat atgagcataa ttttaatgat tcgtcatgcg
     4021 tgagtacaaa tactatcacc cctatgagcc catcggatat tattgttgga ctttataacg
     4081 ataccattaa attaaattta cattttgaat ggaccaataa aaacaacatc acgttgtcaa
     4141 ataatcagag cagtttcacc agtggttatt cggtaacggt gacacctgcg gccagtaatg
     4201 caaaagtgaa tatttctgcg gggggcggtg gttcagtgat gattaatggt gttgcgacat
     4261 tatccagtgc ttcatcatcg acacgcggga gtgccgcagt acaatttcta ctgtgtttat
     4321 taggtggcaa gtcatggggt gcatgtgtaa atagctacag aaatgcattg gcacaaaatg
     4381 caggtgtcta ttccttcaat ctgacattgt catacaaccc gataaccaca acctgcaaac
     4441 aggacgattt attaattact ttagacagta ttcccgtttt acaattacca gccacaggta
     4501 acaaagcaac aataaatagt aaaaaaaggg atattattct gcgttgtaaa aatttattag
     4561 gtcaacaaaa tcaaacatca cggaaaatgc aggtgtattt atcaagttct gacttgttaa
     4621 ccaacagcaa cacaatactg aaaggtgcgg aagataatgc gtaggattta ttcttgaaag
     4681 taatggttcg ccagtcacac ttttaaatat cactaacagc agtaaaggat acacaaattt
     4741 aaaagaaatt gcggcaaagt caaaacttac agatacaacg gtttcaattc cgataacagc
     4801 cagttactac gtctacgata caaacaaagt taaatctggc gcactggagg caaccgcatt
     4861 aatcaacgtg aaatacgact aataacaagc aaaacgagca agtggctaat aataaaaaat
     4921 aacaaggtgt ctgcatttcc ccctcatgat gagggggctt tttttagcga tgataaaaaa
     4981 tctcaccgtc gtaggcttta atgattttac cgtcggtgtc gctgatcaac acgtacgcgc
     5041 cacccatata ggtccagtgc gtcccggcat caggcgcggg caggttacgt aactgccact
     5101 gcttaatgtt gtactcatcg gtgcggtaca tctctgggac ggtatcacct ggtttgaaat
     5161 gcttaaagtc agcaatgaaa ctacttaact catacttaga aattcccgag gtattggtcg
     5221 aacccgcggt ggctggtgcg gcccaggcgg cactggtaac cagcaaaagc acacccagca
     5281 acatcttatt tttaatggtc ataaactctc cactttgggg atctgtaata tcagtcgcat
     5341 atctttcctg tcatacaata taactgacaa gcgatatttt gttagtgatc ccatgatttc
     5401 gtaaaaagtg taaatgaaag gatgccctaa aagcatcctt agcgagcaga gagtcagcgg
     5461 aaatagctga tttaagaatc atcctgggct gaagataaca tgcaagccta aaggattgag
     5521 ttatcgcatt atgcccatga agccatgtac gcccatatac acaccgacta agccaatcaa
     5581 cagactggaa aaataggggg cgcgtttagc gagagtgtta aatccgctcc agcgttttgc
     5641 gacctgctga acgctgattg ctgcgccaac gcctacggtg acaagcgtta acgccaggcc
     5701 aatgctgaaa ctgacgacca gtgttgcgcc cagtgtcagg gctttcaact gaatgcatat
     5761 caacagcacg gtaattgctg ccgggcaggg gataaagcca ccggttaagc caaataacaa
     5821 aatttgccag ttgatgacct ctctaccatc aaagcgtcgt ttaatgtcat tggcatgggc
     5881 tcgtgcatgg gcatcctgat actcgccatg ttcgtgatgg tgatgatgtc cttggtcgtg
     5941 gtgatgttcg tgatcgtgat gatggtgatg atgctcataa tcatgcccgt gcatattctc
     6001 cagccagttg cgttcgccgc gctaggtacg ccagaacatc cagaacgcgg tgccaataat
     6061 gatcactgcg gaaatcagct ggagccacgg ttctgctgat tgagcagtaa agcgcttact
     6121 gatcaccatc ccgccaaagg caatcaacca gaccaccgcg gtatgcgaaa tggttgctgc
     6181 cagtccgagc atcaccgcct gtttaatggt gcctttgatg gcgatgataa acgccgccat
     6241 catcgttttt gagtgccctg gttccaggcc atgaagcgca ccaagtaaga tggcgctggg
     6301 gatgaagaac caggcgtttc cttgctgaag aagagttgta aattcggtca tgataataat
     6361 tcttagtatt aattcggcaa tctgattcta ctccccccca gtacctgata ctaccccccc
     6421 agtagattaa tagtgctatg atttttcata ttcttgttaa ccaggtgttg ccatgtctca
     6481 tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg gccaggtcgt
     6541 ggcgctcaag aaaatgctcg acgagccgca cgaatgcgct gcagttttac aacagattgc
     6601 tgctatccgt ggagcggtaa acggtctgat gcgggaagtg attaaaggtc atctgacgga
     6661 acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg tcgttctgaa
     6721 ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc cgtttttcct
     6781 cgtttacaac gggggcgctg gacattacca tcctcctctg cgatttatca tcgcaaccaa
     6841 acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc tgatctggat
     6901 aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt tatggcagga
     6961 gcaaactatg caagtcgacc tgctgagttc agcgcaatct gcgcacgcgt tacacctttt
     7021 tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa cctttaccgc
     7081 caataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg aagaggccag
     7141 tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga cgcagccacg
     7201 cgcacaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac gctggacgct
     7261 tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg aacttttatc
     7321 ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag ctggcattgc
     7381 taatggcgga cggggagtgg ataccactga cgccgcagct aacgcgatac ccgctgcaca
     7441 aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg attatgttac
     7501 cgatggacat cgtatcattg gtattcacgg tggcgatccg ttaatgacca aagtggtagg
     7561 aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag gcgatacgct
     7621 ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagaacgcg cagtcgccag
     7681 aagcgagggg ccaggcagtt ttgttccaca tttccttgat gcgctctggc aattgacgca
     7741 ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga tccgagtggt
     7801 ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta tggttgctca
     7861 gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta tcgcattgag
     7921 cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat cgataccact
     7981 aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg gttgcaacgt
     8041 tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg cgacccgctg
     8101 ctttcacctt cggcggttgc tacgctgcgc agtcgattat tgccacaggt ttcattaata
     8161 acgccaaact tgcccgaagc tgccgccttg ctcgacgcgc cacacgcgcg caccgaacag
     8221 gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt gctaatgaaa
     8281 ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga gggtgaacaa
     8341 cggtttaccg caccgcgcat tatgaccaaa aacacccacg gcactggttg tacactctct
     8401 gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt acaggaggca
     8461 aaaatctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg tcacggtatt
     8521 ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg ctttggttat
     8581 tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa ttaccagtcg
     8641 aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg caatttatcc
     8701 gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact acatccactt
     8761 ctacggttat cgcccggtta aatctttcgc tattcgcatt cctgccagtt acaccattca
     8821 cggattagat gtttcgcgct ggcaggagcg tatcgactgg cagcgggtgg caaaaatgcg
     8881 cgacaacgat atccgcttac agtttgcttt tattaaggct acggaaggcg aaaagctggt
     8941 ggacccctat ttttcgcgta actggcaatt aagccgcgaa aatggcctgc tgcgcggggc
     9001 gtatcattat ttctccccgt cggtatccgc ttcagttcag gcgcgattat ttctgcaaac
     9061 ggtggatttc tcacaaggcg atttccctgc cgtgctggac gtagaagaac ggggaaaatt
     9121 atcggcaaaa gaattacgca agcgggtaag tcagtggcta aaaatggtcg aaaaacgcac
     9181 gggaaaaaag ccgattattt actcaggagc cgttttttat cacaccaatc tggcgggcta
     9241 tttcaatgaa tatccgtggt gggtggctca ctattatcaa cgtcgtccgg acaatgacgg
     9301 catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca tcaatggtcc
     9361 ggtggatttt aatgtgttta atggcacggg gatgagttgc aggcattcgt tgatgggatt
     9421 aaagaaacgc cttaaataag aagcagtgat atactcccac cagactccct ggcaggaaaa
     9481 agacaaatgg aacaagcgca tacccagctg attgcccaac tgaacgagcg tattttagcg
     9541 gcggataaca cgccgcttta tattaagttt gccgaaacgg taaaaaatgc cgtgcgcagc
     9601 ggggtgctgg agcatggcaa tattttgccc ggtgagcgtg atttaagtca gttaaccggc
     9661 gtttcgcgca ttacggtgcg caaggcgatg caggcgctgg aagaagaggg cgtggtgacg
     9721 cgttcgcgcg gttacggcac gcagatcaac aacatcttcg aatactcgtt gaaagaagcg
     9781 agaggttttt ctcagcaggt ggtattgcgc gggaaaaagc ccgatacgct atgggttaat
     9841 aagcgtgtcg tgaaatgtcc cgaagaagtt gcgcagcagc tggcggccga agcgggaagt
     9901 gatgtctttt tgcttaagcg tattcgctat gtcgatgaag aagcggtatc gattgaggaa
     9961 tcgtgggttc cggcgcattt aattcatgat gttgatgcca tcggaatttc tctttatgac
    10021 tatttccgca gccagcacat ttacccacaa cgtacgcgtt cccgcgttag cgcccggatg
    10081 ccggatgccg agtttcagtc acatattcag ttagatagca aaataccggt gctggtgatc
    10141 aagcaagtgg cgcttgacca acagcaacgg cctattgagt acagcattag ccactgtcgc
    10201 agcgatctat acgtttttgt gtgcgaggag tagttcctcg cgcgttggcg cacaatcacc
    10261 gccccgatgc ccgacaaccc acgacgccac tgcattgccc agtaatacgg catccgccag
    10321 tggcagacca gaggccagcc cggcaagtac gccacctgca tgactgtcgc ccgccccaat
    10381 ggtgtctaca acttgcgttg gaaatgccgg aatgcagcca gaagcgtcgt tgctgaaata
    10441 ccatgcgcct tctttatcga gacgaacgat caacggcgcg gcaaatttct cctgccattg
    10501 cttgccaagt gttgttatct ctgcggataa agcaaaacgt tcggcggcaa tctcagcctc
    10561 ttgacgattg agtgacacta aaggtcgaca ggccatgatc cgcgccagta atgcgtccgg
    10621 gatatcgcca atacgtgggc aaaaatcgat aaacggcgtc acgtcttgca gcttttccag
    10681 ccattccact aacaattcgc cgcagggcga ggccagttga taaccggaaa aatagagcag
    10741 gttgccaggc gcaacggtta atcgtgccaa ccattggcga ttccattgat tttccacacc
    10801 gctgaatgac ataaaagtgc gttcgccatc cggctcaacc agtgccagac accagccgtt
    10861 atcgctttcg gcgttatcga tcaggctgat taacccctct ttcgccatcc ggttgcgaat
    10921 aatctccgcc cacacgccct gaccgagcgg caaggcatta cccgcttcga tgccgaggcg
    10981 ctttaacgcc acggcaatat tcagtgcgca gccgccaacg ttaacgctct gctgtttcag
    11041 ttcgatatcg cacccacgcc aggggagggc ataagcgtcg gcgatcacgt caatgaccgc
    11101 cgcgccgacg accatcaccg gctgacgcgt ggttaattca ggcagcagtg tgtgtaatcg
    11161 agcgccgctc ataccgcctc ccgttgttga cgatatttcg ccagcgctgt ggcatagcgg
    11221 ttgaaatcaa gctgatttac cgcatccagc tccgccttta acgcaggatc gacagcgtta
    11281 atgccatgca acgcgccgca aatcgccgtc gccatagcac ctatggtgtc tgtgtcgccg
    11341 ccaagattgg cgcacaggac ggcgcagcgg tttgggtcgg tttgtgccag ttcaactagc
    11401 gcaatggcgc acggaacgga ctcaatagtg ctggtgcctg cgccaacgac ctggtaaagc
    11461 tgttcgctgg cggattcggt gccgtcggca ttacgcacaa ttttcagcgc aatctccaga
    11521 cgtgccgcca gtgaggcgct gaacgtggtg gtgcgttttt gctgtgcatg tcgcgcaatt
    11581 gaaggcaatg aatcaacaat cgctgaccag cgttctccgt caatggcacg agaaatcgcc
    11641 catgcgatga ctaccgcgcc tgcaaccgcc agatcggatt tatgggtcgg gctggacgcc
    11701 agcgccacat catcaataaa ggaatcaaca tcacgcgctg gaagcaaaca acctaatggc
    11761 gagacgcgca tcgctgcgcc gttggttacg ccgttgtttt ccagttcagc aacgggttta
    11821 ccgtcgcgaa tggcgttaag cgcaatcttc gaggtcggac ctagtacgtt tttgttaaag
    11881 gcgtcgaaac gcaacgccca gtcgagaata ttacgcccaa tcagattcgg atcgatcttg
    11941 ccttcacatt ccagtaacgc atccgccaga cacaggccat cgaggtatcg tcggtgaatt
    12001 cggcgcggtt aaaataacag gccgcgttat tctcctttgg tccaggcaga aaacggtcaa
    12061 tccagccaaa gtgtgcttta acgcggctgc gtggccaaag ctcggagggc atccccatcg
    12121 catcccctaa cgcctgccca taaagagcac cgagaatacg ttctgttttc atttaacttc
    12181 cccttgaatc ggaatttacg ggagcatagt aatgacagat attgcgcagt tgcttggcaa
    12241 agacgccgac aaccttttac agcaccgttg tatgactatt ccttctgacc agctttatct
    12301 ccccggacat gactacgtag accgcgtgat gattgacaat aatcgcccgc cagcggtgtt
    12361 acgtaatatg cagacgttgt acaacaccgg gcgtctggct ggcacaggat atctttctat
    12421 tctgccggtt gaccagggcg ttgagcactc tgccggagct tcatttgctg ctaacccgct
    12481 ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta actgtgtggc
    12541 atcaacttac ggcgtgttgg tgtcggtatc gcggcgctat gcgcatcgca ttccatttct
    12601 cgtcaaactt aatcacaacg agacgctaag ttacccgaat acctacgatc aaacgctgta
    12661 tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg gttggtgcga ctatctattt
    12721 tggctcggaa gagtcacgtc gccagattga agaaatttct gcggcctttg aacgcgcgca
    12781 cgagctgggc atggtgacag tgctgtgggc ctatttgcga aactccgcct ttaagaaaga
    12841 tggcgttgat taccatgttt ccgccgacct gactggtcag gcgaaccatc tggcggcaac
    12901 cataggtgca gatatcgtca aacaaaaaat ggcggaaaat aacggcggct ataaagcgat
    12961 taattacggt tataccgacg atcgcgtgta cagcaagtta accagcgaaa acccgattga
    13021 tctggtgcgt tatcagttag ctaactgcta tatgggccgg gctgggttga taaactccgg
    13081 cggtgctgca ggcggtgaaa ctgacctcag cgatgcagtg cgtactgcgg ttatcaacaa
    13141 acgcgcaggc ggaatggggc tgattcttgg acgtaaggcg ttcaagaaat cgatggctga
    13201 cggcgtgaaa ctgattaatg ccgtgcagga cgtttatctc gatagcaaaa ttactattgc
    13261 ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg ataagacgtt tacactgcat
    13321 ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc atgcctacat agcactgcca
    13381 cgtatgttta caccgcatcc ggcataaaaa cacgcgcact ttgctacggc ttccctatcg
    13441 ggaggccgtt tttttgcctt tcactcctcg aacaattttc atattgttgt ttttgcgatc
    13501 attatctcga catttaaaag caaataattt cattatattt tgaaatcgaa agcaaacgac
    13561 aagatatgaa aatgtacgtg gtatcgacaa agcagatgct gaacaacgca cagcgcggcg
    13621 gttatgcggt tccggcattc aatattcaca atctcgaaac gatgcaagtg gtggtggaaa
    13681 ccgctgccaa cctgcatgcg ccggtcatca tcgccggaac gcctggcaca tttactcatg
    13741 ctggtacaga aaatctgttg gcgctggtca gcgcgatggc gaagcaatat caccatccac
    13801 tggcaattca tctcgaccat cacacgaaat ttgacgatat cgctcagaag gttcgttctg
    13861 gcgtgcgctc agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat atttcacggg
    13921 tcaaagaggt ggtggatttt tgccatcgct ttgatgtcag cgtcgaggcg gagctggggc
    13981 aacttggcgg ccaggaagat gatgtgcaag tcaatgaagt cgatgcgttg tacaccaacc
    14041 ccgcacaggc gcgtgaattt gccgaggcaa ccgggattga ttccctggcg gtcgccatcg
    14101 gcacggctca cggtatgtat gccagcgcac cggtgcttga tttttctcga ctggagaaca
    14161 ttcgccagtg ggtgaactta ccgctggtgc tgcatggcgc gtcaggatta tcgactaagg
    14221 atattcagca aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg gagctgaaaa
    14281 atgccttctt gcagtcgtta aaaaattacc tgaccgagca ccccgaagcg accgatcccc
    14341 gggattattt gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg
    14401 attgtggctg cgagggcagg gcataacgca cctgccattt aacaaggaaa aaacatgaaa
    14461 acgttaattg cccggcataa agctggtgaa catatcggca tatgttcagt ctgttctgcc
    14521 catccgttgg ttatcgaagc ggcgctggca tttgatcgca acagcacgcg caaagtgctg
    14581 attgaagcaa cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat gacaccggca
    14641 gactttcgcg aatttgtttt tacgattgcc gataaagttg ggtttgcacg cgaacgcatt
    14701 attctcggcg gcgatcatct gggaccaaac tgctggcagc aagaaaatgc ggatgcggcg
    14761 atggaaaaat ccgtcgagct ggtaaaagca tatgttcgtg ctggcttcag taaaattcat
    14821 cttgatgcgt caatgtcctg cgcgggggat accataccgt tagcaccaga aacggttgcg
    14881 gaacgagctg ctgtgctttg ctttgctgcg gaaagtgtgg cgacagattg ccagcgtgag
    14941 caactgagct atgtcattgg cactgaagtt ccggttccgg gcggtgaggc cagcgccatt
    15001 cagtcagtac acatcaccca tgttgaagat gccgccaata ctttacgtac gcatcaaaag
    15061 gcctttattg cccgtgggct gacagaggcg ttaacacgtg tgattgccat cgtggtgcag
    15121 ccgggtgtgg aatttgatca cagcaatatt atccattatc agccgcagga agcgcaggcg
    15181 ctggcgcaat ggatagaaaa cacccgaatg gtttatgaag cacattctac cgattaccag
    15241 acccggacgg cttattggga attagtccgc gatcactttg caatattgaa agtcggtccc
    15301 gcattaacct ttgctttacg cgaggcgata tttgcactgg cacaaattga gcaggaactt
    15361 atcgcccccg aaaatcgcag cggttgcctg gcggtaattg aagaagtgat gctcgacgaa
    15421 ccgcaatact ggaaaaaata ttatcgcacg ggttttaacg attcattact ggatattcgt
    15481 tacagcctgt cggatcgtat tcgttattac tggccgcata gtcggattaa aaatagcgtt
    15541 gaaacgatga tggtgaatct gcaaggcgtg gacatcccac tgggcatgat tagtcagtat
    15601 cttcccaaac aatttgaacg cattcagtcc ggggaattat cagcaatgcc gcatcagctt
    15661 attatgaata aaatttatga tgttttgcgc gcctatcgct acggctgtgc ggaataagga
    15721 cggtatatga ctaacctgtt tgttcgtagc ggaatttctt ttgtcgatcg tagcgaagtt
    15781 ttaacctata tcggtaatga gatgctcgcg aaaggtgtgg ttcatgatac ctggccacag
    15841 gcattaattg ccagagaagc agaattccct accgggataa tgcttgagca gcacgccatt
    15901 gcaataccgc attgtgaggc gattcatgct aagtcgtcag ccatttatct gttaaggcca
    15961 acaaataaag ttcattttca gcaagcggat gatgataacg acgtggcggt atcgttggtt
    16021 attgcgttga ttgtggaaaa tccgcagcag caattgaaac ttttacgctg tttatttggc
    16081 aagttacaac agcccgatat cgtcgagaca ctaattactc ttcctgaaac tcagttaaag
    16141 gaatacttca caaagtatgt tttagattca gacgaataaa tccctctgta acaataaaaa
    16201 ggactattta tgaaacgcaa gattattgtc gcttgcggag gcgcggttgc gacctctacg
    16261 atggcggcgg aagaaattaa agagttgtgt cagagtcata atattcctgt tgaattaatc
    16321 cagtgtcggg ttaatgaaat agaaacctat atggatggtg tgcatttgat atgtaccact
    16381 gccagggtgg atcgtagttt tggcgatatt ccgttagttc acggcatgcc ttttgtttct
    16441 ggtgtcggta tcgaagcatt acaaaataaa attctgacta tcttacaggg atgacctatg
    16501 ttttcagaag tcatgcgtta tattctcgac ctcggcccta cagtgatgct gccggttgtc
    16561 atcattattt tttctaaaat attaggcatg aaggcaggcg attgctttaa agcgggtctg
    16621 catatcggga ttggctttgt tggcattggc cttgtgattg gcttaatgct ggattccatt
    16681 ggtccggcgg cgaaagcgat ggcggaaaat ttcgacctga atctgcatgt ggtcgatgtc
    16741 ggctggccgg gctcttcacc aatgacctgg gcgtcgcaaa ttgcgctggt ggcgattccg
    16801 attgcgatat tggttaacgt ggcgatgtta ctgacccgta tgacgcgggt ggtaaatgtt
    16861 gatatctgga atatctggca tatgaccttc accggcgcgt tgctgcacct ggcaaccggt
    16921 tcatggatga tagggatggc gggtgtggta attcacgcgg cgtttgttta taagctcggc
    16981 gactggtttg cccgcgatac ccgaaatttc tttgagctgg aaggtattgc tattccgcac
    17041 ggtacgtcgg cgtatatggg gccgattgcg gtgctggtcg atgctatcat cgagaaaatc
    17101 ccaggcgtta accgaattaa atttagcgcc gacgatattc agcgcaaatt tggccctttt
    17161 ggcgaacctg tcaccgtggg ttttgtgatg gggctgatta tcggcatcct cgcgggttac
    17221 gatgtcaaag gtgtattgca gctggcggta aaaacggcgg cagttatgct gctgatgcca
    17281 cggatgatta aacccatcat ggatggttta acgcccatcg ctaagcaggc ccgtagtcgt
    17341 ttacaggcga agttcggcgg tcaggagttc ctgattggcc tggatcccgc attactgctg
    17401 gggcatacgg cggtggtatc ggcaagcctg atttttattc cgctcaccat tttaattgct
    17461 gtttgtgttc cgggtaatca ggtactgccg tttggcgatc ttgccactat cggcttcttc
    17521 gtggcgatgg cggtcgcggt gcatcgtgga aatctgttcc gcaccttaat ctcgggtgtc
    17581 atcattatga gcatcaccct gtggatcgcg acgcaaacta ttggtttgca cacccaaccg
    17641 gcggctaatg ctggggcgtt aaaagccggg ggtatggtgg cttcaatgga tcagggcggt
    17701 tctcccatta cctggttact gattcaggtt ttctccccgc aaaatattcc cggtttcatt
    17761 attatcggcg caatttatct gaccggtatt ttcatgacct ggcgtagagc gcgtggtttt
    17821 actaaacaag agaaagccgt tctcgcagaa taatttttac ctgaggggga ggttatcccc
    17881 cttaacaa
//
LOCUS       DAZOYF010000074        17865 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZOYF010000074 DAZOYF010000000
VERSION     DAZOYF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.074"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..233
                     /locus_tag="ACHUOF_002363"
     CDS             <1..233
                     /locus_tag="ACHUOF_002363"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611515.1"
                     /translation="CAVRLTNLRTTRRTCEDLSILSFLVFIFHFLFSSLSGSVLVLVF
                     SFLFLFFLQGYAKTPKHFSPQKHPIILPDQSP"
     gene            complement(304..1677)
                     /gene="eutA"
                     /locus_tag="ACHUOF_002364"
     CDS             complement(304..1677)
                     /gene="eutA"
                     /locus_tag="ACHUOF_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR0611516.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(1652..2131)
                     /gene="eutP"
                     /locus_tag="ACHUOF_002365"
     CDS             complement(1652..2131)
                     /gene="eutP"
                     /locus_tag="ACHUOF_002365"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR0611517.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(2144..2479)
                     /gene="eutS"
                     /locus_tag="ACHUOF_002366"
     CDS             complement(2144..2479)
                     /gene="eutS"
                     /locus_tag="ACHUOF_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR0611518.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(2772..5051)
                     /gene="maeB"
                     /locus_tag="ACHUOF_002367"
     CDS             complement(2772..5051)
                     /gene="maeB"
                     /locus_tag="ACHUOF_002367"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR0611519.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDSLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            5340..6290
                     /gene="tal"
                     /locus_tag="ACHUOF_002368"
     CDS             5340..6290
                     /gene="tal"
                     /locus_tag="ACHUOF_002368"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0611520.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            6310..8313
                     /gene="tkt"
                     /locus_tag="ACHUOF_002369"
     CDS             6310..8313
                     /gene="tkt"
                     /locus_tag="ACHUOF_002369"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0611521.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(8416..9459)
                     /locus_tag="ACHUOF_002370"
     CDS             complement(8416..9459)
                     /locus_tag="ACHUOF_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR0611522.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(9585..10160)
                     /gene="nudK"
                     /locus_tag="ACHUOF_002371"
     CDS             complement(9585..10160)
                     /gene="nudK"
                     /locus_tag="ACHUOF_002371"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR0611523.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(10228..12207)
                     /gene="aegA"
                     /locus_tag="ACHUOF_002372"
     CDS             complement(10228..12207)
                     /gene="aegA"
                     /locus_tag="ACHUOF_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR0611524.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            12413..14130
                     /gene="narQ"
                     /locus_tag="ACHUOF_002373"
                     /pseudo
     CDS             12413..14130
                     /gene="narQ"
                     /locus_tag="ACHUOF_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            14332..17445
                     /gene="acrD"
                     /locus_tag="ACHUOF_002374"
     CDS             14332..17445
                     /gene="acrD"
                     /locus_tag="ACHUOF_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR0611525.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(17544..17603)
                     /gene="ypfM"
                     /locus_tag="ACHUOF_002375"
     CDS             complement(17544..17603)
                     /gene="ypfM"
                     /locus_tag="ACHUOF_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR0611526.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         4212 a         4753 c         4605 g         4295 t
ORIGIN      
        1 attgtgcggt cagactaaca aacttgcgta ccacccgaag aacatgcgaa gatttaagta
       61 ttctttcttt tttggttttt atttttcatt ttctcttttc ttctctttcc ggttctgttc
      121 tggttttggt tttttccttt ttatttttat tctttcttca gggttatgct aaaacaccaa
      181 aacatttttc accacaaaag catccaatca tcttgccaga ccaatcacct taaccggata
      241 cactgccgga tataatccca ctaacaccca gacaccaagc accatacctg ataactaccg
      301 tattcaggaa ggaaatgcga gtgatttcac cgtcaccggc acaaccgatc cgccaaaaag
      361 aggcgtacca atgtcgatat agtcccccgc gcggacaatc acttcgtcaa tgactgccaa
      421 cgggagttgt tgtagctgtg ggcgcaacaa catgcccaga gctttaccaa agtcctgccc
      481 ggccaccacc agcaggggat gcggattcgg aaaacgcgcg acgaaatcga ccagcgcgtt
      541 gatgaccgtc agaaccgcgg cgtaacgcac aggcagcgag gcgggaagcg ccagcacgta
      601 cacgtcagtt ttgggatcaa gatccagctg aagcagcgcc tgttgccagg cactcaccag
      661 atccgtttca tcaatcggga tcgccaccgg caaattgcgc agcggcagtt gtacgccctc
      721 cagccagatt gtgctgccag agagcgaaag ggtatgtgca cccgcgccaa tcaccgtgga
      781 gcgtacggtt tgcgccggaa actgcacatt catctcacgc aggcgcggat ggtcatgcag
      841 cgccgttgcc agaagtgggc caatatcggc aaaacagaac gggttggcgg gctggtggcg
      901 ataacattcg cccacgcctc cagaaagcgt aatgatttcg ggcgtaacac ctgcgggcag
      961 caaaccggtt tgcatcaatg cctgcgcgag cggcgaaagc gttccgtcaa tcacttcgac
     1021 aatcagcgcc gccatccgcc gggtaacctg caccagctgc gcgccggtca gcgaacgggc
     1081 gtcagtgcct gcaccgaagc actcatccac aatcatctgc cccggtttat gagcgtaaac
     1141 cacgcgcccc tggctgtcgg tttccagcag gcgaccaccg acgttgagac aggcagtgcc
     1201 gctgattttt ccggcatcga acagggcgta gttcgcggtg ccaccgccga tatcgatatt
     1261 cagtacccga cacagccgtt gttcagaaag ggtttgcgcc ccggctccgt gaccggcgat
     1321 cacggattcg aggtgcggcc cggcgctggc gacgacaaaa tcgcccagcg actgtttttc
     1381 cttcatcact ttttccatca gctgtgcgac caggctttcg gtaaactggc cttccggcag
     1441 ctgggcaatg atggtttcgc ggatgcgctg gctttcggtt ttgtcggctg gcacgctggc
     1501 gggcacggac gccgttaccg ggatcgattc atcacattcg gtgatggtaa aacccagtag
     1561 atccgccact tcacgggctt ccggggtaat aatgctggcg cgcagaacca cggacattgc
     1621 ctgttcgccg cgcgcgtgtg cttcacgaat atcattcgct gtgatgagtt ttttcacctg
     1681 cttcctcctg tttggtgaga gatgccagat aatccaccag ttgctgcacg ctttgcgggt
     1741 cgtggctgtt tagctcgaaa atcggctctt cgaaacccgt ctcaagcaat aactttcgcg
     1801 ttgcggcgac gtcagcatct ggcatgtccg ttttactgat gacggcgatt tgccgtttgc
     1861 tgacgccaat atccagcaac ccggcaggca accgactttc cgggtcattt gcgccatgga
     1921 catagatcag catgtctaca tcctgtagcg tggtaattaa ggcgtgatac cagcggggat
     1981 ggctaaaata ttcacccggc gtatcaatat cgcccttatc attaaattcc acggccagtg
     2041 tttttctggc gagggtataa ttcccctgca acgcattaaa taacgttgtt tttcctgccc
     2101 cgacgctacc cacaaacgca attcgtttca tgaatgcctc gtattaactt ttggtcattt
     2161 cacaaagcgt ataatttaat aagcgaccta aaccgctgac ggtttgtgat aacgcttctt
     2221 ctactgcgcc caccgagcca taaatcacca atgcaccgct gaagcggtcg agaaaaccga
     2281 tatgcacatc agcggctttc agcgctaaat caccggcgat catcgccgtt tcgccgggag
     2341 ttagcgtcat aatgccaatt gcgcccgcat ccggaacgcc gattttcttc gccagttcct
     2401 caccgggatg cgcgatgaga tgcgccagtg tgacctgttt gccgggcaca aattcctgaa
     2461 tgatgcgttc tttatccatg agtcgccgcc tgtgaaaaac tttcgtgtat cgtgacaatt
     2521 tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg tagatcaata
     2581 agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat gaatataaat
     2641 aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac tcagtaataa
     2701 gctttgctaa tatatttcag gaaaaccgct taccctgaat attcagggta agcgtgagag
     2761 ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc caccatattg
     2821 acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc cacgcccatc
     2881 agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt gtaactaatg
     2941 cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa agggctgtcc
     3001 ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat ttcaccatca
     3061 atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat tttgctcgac
     3121 gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg cggctcaata
     3181 ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc ttctgcgtct
     3241 ggttcgtcat tgacgtaggt atcggcaata aaggtgttac cactcggcag cagcagcgca
     3301 ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt caccacacta
     3361 aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc cccacgctga
     3421 accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc ctgttcctga
     3481 gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt aaagcgcgga
     3541 tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa gcccagtttc
     3601 tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc cagtcccagc
     3661 gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg cagaactacg
     3721 cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt ggttttgtaa
     3781 acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg cgtcgccacg
     3841 cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa gcgcggatca
     3901 aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc atacgctgaa
     3961 gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac cgccgccagt
     4021 ttcatctctt cgttgatggc ggttgcgcca acgtccagcg cgccacggaa gatgaacggg
     4081 aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca gatgatggca
     4141 tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt cgccagcgcc
     4201 aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac tttcgggccg
     4261 gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg tttgccgtca
     4321 tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacggcc ctgatagata
     4381 acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc taccagcagg
     4441 ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac gtcggagatg
     4501 tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat tgccgtgccg
     4561 tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg ttcaatatag
     4621 aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg ttcgagcgcg
     4681 gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc aaatacatca
     4741 atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt gcctgccagc
     4801 gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc aaccaggtta
     4861 ccgcgcgcgg tatatttgta ggcttttagc gagtcttttt cgatttcaag acaaggtgcg
     4921 gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg cttggttgga
     4981 gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact ttgttttaac
     5041 tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt gtgggcaaca
     5101 gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga ctttgagcaa
     5161 gtccaaactc tcaccattaa tataatgttt tggtaataat cctataacac tgatgttacc
     5221 tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt tagcgaaatt
     5281 tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga gtatttccta
     5341 tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc tgcgatattg
     5401 agtccattcg ccattatcat ccccaggatg ccaccactaa tccttcgctg ttactcaagg
     5461 ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg ggtaaaaaaa
     5521 atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg gtcaatttcg
     5581 gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat gcacgcctct
     5641 cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg tatcagcaac
     5701 aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa ggaattcgcg
     5761 cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg ttttctttta
     5821 cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt gtagggcgta
     5881 tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa gaagatccgg
     5941 gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat gaaaccattg
     6001 tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc tgcgatcgac
     6061 tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca gtggtacgta
     6121 aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa gcggagttcc
     6181 gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc attcgtctgt
     6241 tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa accagccacg
     6301 gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag tatggatgct
     6361 gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga tattgccgaa
     6421 gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta tgatcgcgac
     6481 cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct acatctgacc
     6541 ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc gaaaacccca
     6601 ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc gcttggacaa
     6661 ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc gcagtttaac
     6721 cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga cggctgcctg
     6781 atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact aggcaagctg
     6841 attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg ctggtttacc
     6901 gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga aatcgacggt
     6961 cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa agataagccg
     7021 tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc aggtaaggaa
     7081 gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca aaaactgggc
     7141 tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga tgcccgtgaa
     7201 aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa aaaggctcat
     7261 ccgcaactgg cagaagagtt tccccgacgg atgagcggtg gtttaccgaa ggactgggag
     7321 aaaacgactc agaaatatat caatgagtta caggcaaatc cggcgaaaat cgctacccgt
     7381 aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt gctcggcggt
     7441 tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc tctgaaggaa
     7501 gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac cgctatcgcc
     7561 aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgttcct gatgtttgtt
     7621 gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca gattatggtt
     7681 tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc tgttgagcaa
     7741 ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga tcagatggaa
     7801 gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc actgatcctc
     7861 tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga gattgctcgt
     7921 ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat tgccaccggt
     7981 tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg tcgcaatgta
     8041 cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga atatcgggag
     8101 tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat tgccgattac
     8161 tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta cggggaatct
     8221 gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat tgtggcaaaa
     8281 gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc tgtatgttcc
     8341 cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt tttagggccg
     8401 catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg gcccatgcca
     8461 gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac cgtcaaagcg
     8521 ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg tcaccagttc
     8581 acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct cctggccgtt
     8641 gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac gcgacacaat
     8701 ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca tcagtgcttt
     8761 atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg agcagcgcag
     8821 accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga gtgagagtgg
     8881 cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg gtacgctgag
     8941 aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct tttgctgagc
     9001 atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat tgccgtcgcg
     9061 taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg ttgccgtatc
     9121 atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg ccaacggctc
     9181 accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg acttcaaccc
     9241 accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc tcagggtcat
     9301 caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat tattacaggt
     9361 gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag acgtcggcaa
     9421 caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga ccccagaagt
     9481 tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca atcggatttg
     9541 tcggattgag ccaataataa acaacgaaat attattgcta tttttcagtc cattaaatgt
     9601 gacatttgca aatagttaag caataacacc gtcttaccgt cacgtatctc gccggttttg
     9661 atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc atcttcaaca
     9721 ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa gtggattagc
     9781 tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc gcccacttca
     9841 tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc gttatccagc
     9901 agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca ggtagcgaca
     9961 cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag gatcgtcgca
    10021 ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc tttgcgcgtg
    10081 agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt gtctttaatg
    10141 agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta tattacgctg
    10201 tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta cccccagcca
    10261 gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca ccagatccgc
    10321 accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt aacggtactg
    10381 gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt gcgactccag
    10441 ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg ctggcatgac
    10501 aaattcactg ccctcaatcg gtacaggtcg ccgacgcccc tgggcatccg gctcgccaag
    10561 acgcgtgcgc aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct caagcgccac
    10621 cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca cctctttctt
    10681 cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac tcgcgccgtg
    10741 gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta ccacgacgtt
    10801 aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca tcacctgttt
    10861 agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt cattgggtaa
    10921 acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt attgttccaa
    10981 aagcaaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt gaatccccat
    11041 cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga aagaaggaat
    11101 gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca ccgccacgcc
    11161 attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga taatcgccac
    11221 ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg ccaacgcctg
    11281 atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat cgcgaatagt
    11341 acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt ccggcagggt
    11401 gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca ccagttcaat
    11461 ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt gctcgccgaa
    11521 cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca gataaatttc
    11581 atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac gcgcgggcgt
    11641 tgcctgcatt tgttcgactt tactcattac cggcatttct tgtgcagcgg tactggcatg
    11701 ccacggttga tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca tgccggagag
    11761 tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac aggcaggacc
    11821 gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt ttcctgccgc
    11881 gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc aggatttaca
    11941 gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga ttgcgccatt
    12001 agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg cactacgttg
    12061 ctgctgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca ggacatgttg
    12121 ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca gacattgctg
    12181 actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta aaaaccttat
    12241 tctttatatg agtgttgtta ttacccgact tacaggggaa ccggcaatgt tcatgtgccc
    12301 agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat cactgactaa
    12361 attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc gtgtgattgt
    12421 taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc tgctgtcgat
    12481 tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg ctgaggctat
    12541 caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact tgcaaagtgg
    12601 cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt caccggtatt
    12661 aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg cgcatctgaa
    12721 tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt ggtatcaggc
    12781 caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac agcactacgc
    12841 tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg gtattttcac
    12901 gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc tgaatcagct
    12961 ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc tggataccag
    13021 tctgccgaat gagcttggtc tgcttgcaaa aacctttaac caaatgtcga gcgagctgca
    13081 taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc tccacgagac
    13141 caagcgtcgt ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa caccagccag
    13201 attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga agcagctgaa
    13261 tatctggagc tgaacgttgg ggataactgg cggattagcg aagggaagcc aaaccctgag
    13321 ctgccaatgc agattttacc ggtgacaatg caagagacgg tttacggcga actgcactgg
    13381 caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc gatgctggga
    13441 cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt gatggaagaa
    13501 cgtgcgacca tcgcccgcga attgcacgac tcgctggctc aggtgctttc ttacttacgt
    13561 atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc acaaagtatc
    13621 atggccgatt tttcccaggc gttgaatgat gcttatcggc agttacgcga gctgttgact
    13681 actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga aatgctggat
    13741 acgttacaaa atcaaaccag cgctaaactg accctcgact gccgtctgcc aaccctggca
    13801 ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt gctgaatgcg
    13861 atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc ggacggcaat
    13921 cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga acccgaaggt
    13981 cattatggac tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac gctgactttt
    14041 tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctccgc agagggtgag
    14101 gggaagggga acacgccggc tgaggtgtaa ataattaccc tcacaccggc cctctccctt
    14161 taaaggcgag agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt cagcaatctc
    14221 atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt gccgctacag
    14281 tgaagcaagt caagcctaca acaatacgca gaaacacgag gtcctctttt aatggcgaat
    14341 ttctttattg atcgccccat ttttgcctgg gtgctgacaa tcctgttgtg tctgacaggt
    14401 accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc accgaatgtg
    14461 cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac cgtgacccag
    14521 gttatcgagc aaaatatgac cggcctcgat aatctgatgt atatgtcatc tcagagcagt
    14581 ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc ggacgaagcc
    14641 gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca ggcggtgcaa
    14701 aatcagggcg tgacggtgcg taaaaccggc gataccaaca ttctgaccat tgccttcgtc
    14761 tctaccgatg gctcgatgga taaacaggat attgctgatt atgttgccag taatattcag
    14821 gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc gcaatattcc
    14881 atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc taaagatgtc
    14941 actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg tggtacacct
    15001 tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct gcaaacacca
    15061 gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaggt aaggctgggc
    15121 gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg cttcaatggt
    15181 aagccagcct ccgggttggg ggtaaaactg gcctccagcg caaacgaaat ggcgacagcg
    15241 gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg actggaatac
    15301 aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt ggtgaaaacg
    15361 ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca aaacttccgc
    15421 gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt ctccgtactt
    15481 tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct ggcgatcggt
    15541 ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat gagtgaggaa
    15601 ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg ggcactggtc
    15661 gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg cggcaccacc
    15721 ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct gtcagtactg
    15781 gtagcgatgc tcctcactcc ggctctgtgt gccacgctac ttaagccact gaaaaaaggt
    15841 gagcatcacg ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa ccgcaacgcc
    15901 gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg gattgtgatt
    15961 tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac gtcgttctta
    16021 ccgctggaag accggggcat gtttactacc tcggtacaat tgcccagcgg ttcaacccaa
    16081 caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca tgaaaaagac
    16141 aacatcatgt cggtgtttgc caccgttggt tctggccctg ggggtaacgg gcaaaacgtg
    16201 gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac cggcacctcg
    16261 tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc tcgcgttatc
    16321 gccagcagcc caccagcaat tagcggtctt ggtagttctg caggttttga tatggagttg
    16381 caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaatca gttgctggcg
    16441 ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga tgacagtccg
    16501 cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga tatcgacgat
    16561 attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt tatggatcgc
    16621 ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct gccagatgac
    16681 atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc tgctttcgcg
    16741 acctcacgct gggaaacagg ctcgccgcgt ctggaacgct ataacggtta ttctgcggtt
    16801 gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga tattatggaa
    16861 tcgttagtga agcagctgcc aaacggcttt ggtctggagt ggacggcgat gtcgtatcag
    16921 gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct ggtggtattc
    16981 ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt tctcggtaat gctggtcgtg
    17041 ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga aaacgacgtt
    17101 tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc catcctgatc
    17161 gtcgagtttg ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc gacgctccac
    17221 gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat cttcggcgta
    17281 ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt gggtactggc
    17341 gtaatgggcg ggatgatttc agctactatt ctggctattt acttcgtgcc gctgttcttt
    17401 gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat aaaaaaggcg
    17461 acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag cgggttcttg
    17521 ctgtttatac tttgtctcag gaattactta cgaagcataa cttcgataaa gtctttccag
    17581 ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat tctacggaaa
    17641 caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc cgggggacaa
    17701 accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac agcaacattt
    17761 cgagatattc atacggcatc taatactgat ttaattctgg ttaaaataca gacagataac
    17821 aagatgaata ttcttaatgt ttacgttaaa aatgtttaat attat
//
LOCUS       DAZOYF010000075        17747 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZOYF010000075 DAZOYF010000000
VERSION     DAZOYF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.075"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            143..826
                     /locus_tag="ACHUOF_002376"
     CDS             143..826
                     /locus_tag="ACHUOF_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR0611527.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            870..5532
                     /locus_tag="ACHUOF_002377"
                     /pseudo
     CDS             870..5532
                     /locus_tag="ACHUOF_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5900..7555
                     /gene="lldP"
                     /locus_tag="ACHUOF_002378"
     CDS             5900..7555
                     /gene="lldP"
                     /locus_tag="ACHUOF_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR0611528.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7555..8331
                     /gene="lldR"
                     /locus_tag="ACHUOF_002379"
     CDS             7555..8331
                     /gene="lldR"
                     /locus_tag="ACHUOF_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR0611529.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8328..9518
                     /gene="lldD"
                     /locus_tag="ACHUOF_002380"
     CDS             8328..9518
                     /gene="lldD"
                     /locus_tag="ACHUOF_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR0611530.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9714..10187
                     /gene="trmL"
                     /locus_tag="ACHUOF_002381"
     CDS             9714..10187
                     /gene="trmL"
                     /locus_tag="ACHUOF_002381"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR0611531.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10240..11061)
                     /gene="cysE"
                     /locus_tag="ACHUOF_002382"
     CDS             complement(10240..11061)
                     /gene="cysE"
                     /locus_tag="ACHUOF_002382"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR0611532.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGCGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11141..12160)
                     /gene="gpsA"
                     /locus_tag="ACHUOF_002383"
     CDS             complement(11141..12160)
                     /gene="gpsA"
                     /locus_tag="ACHUOF_002383"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR0611533.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12160..12627)
                     /gene="secB"
                     /locus_tag="ACHUOF_002384"
     CDS             complement(12160..12627)
                     /gene="secB"
                     /locus_tag="ACHUOF_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR0611534.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12690..12941)
                     /gene="grxC"
                     /locus_tag="ACHUOF_002385"
     CDS             complement(12690..12941)
                     /gene="grxC"
                     /locus_tag="ACHUOF_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR0611535.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13083..13514)
                     /locus_tag="ACHUOF_002386"
     CDS             complement(13083..13514)
                     /locus_tag="ACHUOF_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR0611536.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13759..15303
                     /gene="gpmM"
                     /locus_tag="ACHUOF_002387"
                     /gene_synonym="pgmI"
     CDS             13759..15303
                     /gene="gpmM"
                     /locus_tag="ACHUOF_002387"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR0611537.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15337..16596
                     /gene="envC"
                     /locus_tag="ACHUOF_002388"
     CDS             15337..16596
                     /gene="envC"
                     /locus_tag="ACHUOF_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR0611538.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16600..17559
                     /locus_tag="ACHUOF_002389"
     CDS             16600..17559
                     /locus_tag="ACHUOF_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR0611539.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17546..17747)
                     /locus_tag="ACHUOF_002390"
                     /pseudo
     CDS             complement(17546..17747)
                     /locus_tag="ACHUOF_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4438 a         4613 c         4643 g         4053 t
ORIGIN      
        1 tacattttta tgatgaatgc ttaaaacaat atttaatcga cacacatcac tgtcattaat
       61 gtaaagcaac atagctcacc aataatacaa tggacaactc taccggcatt gagcgctggt
      121 cggaggaaat aaggattttt acatgaactt gaagaagatt tttttcagtg ctgtcactgt
      181 ttcagttctc tgcgcattaa cgggatgtga ctacattgaa gaaggcaagc cagaaagtag
      241 tttactgaaa caacaagaag aacataataa taaaattgtg ctacttgaaa aacaacaagc
      301 acagctaaag agtcaactcg aaactattca aaaacaacaa actggcataa taaacagtac
      361 aaagacttta actcacgtaa ttaaatccgt taaagaccaa caaaatactt ttatttttac
      421 cgagtttaac ccagcaaaaa cgaagtattt catcctgaac aacggttcgg tggctttagc
      481 gggtcgagtg ttatccattg acgccacaga gaatggtagt gttattcata tctcactggt
      541 caacttatta agtacaccta tctcaaacat tggttttaat gcgacatggg gtggtgaaaa
      601 acctgtcgat gccaaagagt ttgcccggtg gcaacaatta cttttcaaca catcaatgaa
      661 atccacattg aaattattac caggtcaatg gcaagacatt aatttgaccc tgaagggtgt
      721 atcgcccaat aacctgggat atctgaagtt agccatcaac atggaaaata ttcagttcga
      781 caatcttccc tctgctgaaa atcggcagaa aagaagcaaa aaataaatgc agttagtacg
      841 cttataacaa ggaatagcga gcatcattaa tgaacaaaat atttaaagtt atctggaacc
      901 ctgcgacagg gaattatact gttaccagcg aaacggcaaa aagccgtggc aagaaatctg
      961 ggcgcagtaa gctgttaatt tctgcgctgg ttgcgggtgg aatgttgtcg tcgtttgggg
     1021 cattggcgaa tgccgggaat gacaacggtc agggtgttga ttacggtagt ggatcagctg
     1081 gcgacggctg ggttgctata ggcaaagggg cgaaagcaaa tacttttatg aacaccagtg
     1141 gttccagtac tgctgtgggt tatgacgcta tagctgaagg ccaatatagc tctgccatcg
     1201 ggtcaaaaac ccatgcgatt ggtggtgcat caatggcctt tggggttagt gcaatatcag
     1261 aaggcgatag aagtatagca ctgggtgcct cttcgtattc attgggccaa tactcaatgg
     1321 ccctcggccg ttattcaaaa gcattgggta aattgtctat tgctatgggg gactcttcca
     1381 aagcggaagg agcaaacgcc attgccctgg ggcgtagcag tgtagctagc ggtactgaca
     1441 gcctcgcatt tggcagacaa tcacttgcca gcgcagcgaa cgctattgcg ataggtgctg
     1501 agaccgaagc cgctgaaaat gcaactgcta ttggcaataa tgcgaaggca aaagggacta
     1561 atagcatggc aatggggttc ggaagccttg ccgataaagt caatactatc gcattaggaa
     1621 atggcagcca ggctctggca gataatgcaa tcgccatagg ccagggcaac aaagctgatg
     1681 gcgtggatgc catcgctctg ggtaatggta gccagtcgag aggcttaaac accattgcct
     1741 taggcacagc cagtaatgca actggtgata agagtcttgc gcttggtagt aatagcagtg
     1801 ccaacggtat taactctgtc gcgctgggcg cagattccat tgcggattta gacaataccg
     1861 tctctgtcgg caatagttca ttaaaacgca agatcgttaa tgtgaaaaat ggcgcgatca
     1921 agtctgacag ttacgatgcc attaatggtt cacagcttta tgccattagc gactcggtag
     1981 caaaaaaggc ttggaggagg ggctgcagta gatgttgatg acggtactgt tacagcacca
     2041 acctacaatt taaaaaatgg tagcaaaaat aacgtagggg ctgcgctcgc tgtacttgat
     2101 gaaaacaccc tgcaatggga ccaaaccaaa ggcaaataca gcgctgctca tggtactagt
     2161 agcccaactg ccagcgtaat caccgatgtt gcggatggca cgatttcagc ctccagtaag
     2221 gatgcggtta acggttccca actgaaagct accaatgacg atgtcgaagc caacaccacc
     2281 aatatcacca acctgacgga ttccgttggt gaccttcagg ctgatgccct gctctggaac
     2341 gaaactaaaa aggcattcag tgcagctcac ggccaggata ccaccagcaa aatcaccaac
     2401 gttaaagatg ccgacctgac ggctgacagc actgatgctg ttaacggctc tcagctgaaa
     2461 accaccaacg atgctgtggc gacgaatacc accaatatcg ccaataacac ttccaatatt
     2521 gccactaaca ccaccaacat ctctaacctg actgagacgg tgactaatct tggtgaggat
     2581 gcgctgaaat gggataagga caatggtgta ttcacggcag ctcatggcac cgagaccacc
     2641 agcaaaataa ccaacgttaa agatggcgac ctgacgactg gcagcaccga tgccgttaac
     2701 ggctctcagc tgaaaaccac caacgatgcc gtggcgacga ataccaccaa tatcgccact
     2761 aacaccacca acatctctaa tctgactgag acggtgacta atcttggtga ggatgcgctg
     2821 aaatgggata aggacaatgg tgtcttcact gcagctcatg gcaacaatac cgccagcaaa
     2881 atcaccaata tcctggacgg cacagtcact gcaaccagtt ccgatgccat taacggtagc
     2941 cagctttatg acttaagcag caatatcgcc acctacttcg gcggcaatgc ttctgtgaat
     3001 actgacggtg tgtttaccgg tccaacctac aaaatcggtg aaacaaatta ttataacgtc
     3061 ggcgatgcac tggctgcgat taactcctca tttagcacgt ctctcggcga tgctctgctt
     3121 tgggatgcca ccgcaggtaa attcagtgcc aaacacggta ctaatggtga cgcaagcgtg
     3181 atcactgatg tcgcagatgg tgaaatttca gactccagtt ctgacgcagt aaacggctca
     3241 caactccacg gcgtgagcag ttatgttgtt gatgcgctgg ggggtggtgc cgaagtcaat
     3301 gcagacggca ccatcactgc gccgacgtac accattgcta atgctgatta cgataatgtc
     3361 ggtgatgccc tgaatgctat cgataccact cctgacgacg ctctgctctg ggatgcggac
     3421 gccggtgaaa atggtgcatt tagcgccgct cacggaaaag ataaaactgc cagtgtaatc
     3481 actaacgtcg ctaacggtgc aatctctgct gccagcagcg acgcgattaa cggctcacaa
     3541 ctctatacca ccaataagta catcgctgat gcgctgggtg gtgacgcaga agtcaacgct
     3601 gacggcacca tcaccgcacc gacttacacc attgcgaacg ccgagtacaa caacgtcggt
     3661 gacgccctgg atgcgcttga tgataacgcc ctgctgtggg ataagactgc caatggcggt
     3721 gctggagcct acaatgccag ccatgacggt aaagccagca tcatcactaa tgtcgctaat
     3781 ggcagtatta gtgaggacag taccgatgca gtgaacggtt ctcagttgaa tgcgacgaat
     3841 atgatgattg agcagaacac ccaaattatc aatcagctcg ctggtaacac cgacgcaacc
     3901 tatatcgaag aaaatggcgc tggtatcaac tacgttcgta ctaacgacaa cgatttagcc
     3961 tttaacgatg caagcgcctc tggtgtcggc gctacagctg taggttataa cgctgtcgcg
     4021 tctggtgcca gcagcgtagc cattggtcag aacagcagca gcaccgttga taccggtatt
     4081 gcgctgggta gcagctccgt ttccagccgt gtgattgcca aaggttctcg tgacactagc
     4141 gtaacggaaa atggcgtggt gattggttat gacaccactg acggcgaact gctaggtgca
     4201 ttgtcaattg gtgatgacgg taaataccgc caaatcatca acgtagctga tggttcagaa
     4261 gcccatgacg ccgttacggt tcgccagttg cagaacgcta ttggagcggt cgccactacg
     4321 ccaaccaagt actttcacgc caactcaacg gcagaagact cactggccgt tggtgaagac
     4381 tcgctggcaa tgggtgcgaa aactgtcgtt aatggtaatg caggtattgg tatcggtttg
     4441 aacactctgg ttctggctga tgcgatcaac ggcattgcta tcggcagtaa cgcacgcgca
     4501 aaccatgcaa acagtatcgc aatgggtaac ggttctcaga ccacccgtgg tgcacagact
     4561 ggctacaccg cctacaacat ggacgcaccg cagaactctg taggtgagtt ttctgtcggt
     4621 agtgaagacg gtcaacgtca gatcacaaac gtcgcagctg gttcagcgga taccgatgcg
     4681 gttaacgtgg gtcagttgaa agtcactgat gagcgcgtag cgcaaaatac ccagagcatt
     4741 actaacctga acaatcaggt cactaatctg gatactcgcg ttactaatat cgaaaacggt
     4801 attggcgaca ttgtcaccac cggtagcacc aagtacttca agaccaacac cgatggcgta
     4861 gatgccaacg cccagggtaa agatagcgtt gctattggtt ctggttccat tgctgccgct
     4921 gacaacagcg tcgcactggg taccggttcc gttgcagagg aagaaaatac aatctctgta
     4981 ggttcttcta ctaaccaacg ccgtatcacc aacgtagctg ccagtgttaa tgccaccgat
     5041 gcggttaacg tgtcgcagct gaaatcttct gaagcaggcg gagtgcgcta cgacaccaaa
     5101 gctgatggtt ctatcgacta tagcaatatc accctcggtg gtggcaacgg cagtacgact
     5161 cgtatcagca acgtctccgc tggcgtcaac aacaacgacg cggtgaacta cgcgcagttg
     5221 aagcaaagcg cgcaggaaac gaagcaatac accgatcagc ggatggttga gatggataac
     5281 aaactgtcta aaactgaaag caagttgagc ggtggtatcg cttctgcaat ggcaatgacc
     5341 ggtctgccgc aggcttatac accgggtgcc agcatggctt ctattggtgg cggtacttac
     5401 aacggtgaat cggcagttgc tttaggtgta tcgatggtga gcgccaatgg tcgttgggtc
     5461 tacaaattac aaggtagtac caatagccag ggtgaatact ccgccgcact cggtgccggt
     5521 attcagtggt aatgatccat taagttagtg tgactaaggg caaacgccaa attgcctgat
     5581 gcgttacgcg catcaggcaa gataaggcgc tccgggtcaa caatctaagg gcaattctct
     5641 gatgaggatt gcccttttct ttaccagaca tctcccccca caagaattgg ccctaccaat
     5701 tcttcgctta tctgacctct ggttcacaat tccccaatta aaactcacat caatattgcc
     5761 aatacataac atttagttaa ccattcattg tcattatccc tacacaacac aattggcagt
     5821 gccactttta cacaacgtgt gacaaggaga tgagcaacag actcattaca cgatgtgcgt
     5881 ggactctagg agacctgcaa tgaatctctg gcaacaaaac tacgatcccg ccgggaatat
     5941 ctggctttcc agtctgatag catcgcttcc catcctgttt ttcttctttg cgctgattaa
     6001 gctcaaactg aaaggatacg tcgccgcctc atggacggta gcaatcgccc ttgccgtggc
     6061 tttgctgttc tataaaatgc cggtcgctaa cgcgctggcc tcggtggttt atggcttctt
     6121 ctatggtttg tggcccatcg cgtggatcat tattgcagca gtgttcgtct ataagatctc
     6181 ggtgaaaacc gggcagtttg acatcattcg ctcgtctatt ctttcgataa cccctgacca
     6241 gcgtctgcaa atgctgatcg tcggtttctg tttcggcgct ttccttgaag gagccgcagg
     6301 ctttggcgca ccggtagcaa ttaccgccgc attgctggtc ggcctgggtt ttaaaccgct
     6361 gtacgccgcc ggactgtgcc tgattgttaa caccgcgcca gtggcatttg gcgcgatggg
     6421 cattccaatc ctggttgccg gacaagtaac aggcatcgac agctttgaga ttggccagat
     6481 ggtggggcgt cagctaccgt ttatgaccat tatcgtgctg ttctggatca tggcgattat
     6541 ggacggctgg cgcggtatca aagagacgtg gcctgcggtc gtggttgcag gcggctcgtt
     6601 tgccatcgct cagtacctta gctctaactt cattgggccg gagctgccgg acattatctc
     6661 ttcgctggta tcactgctct gcctgacgct gttcctcaaa cgctggcaac cagttcgcgt
     6721 attccgcttc ggtgacttgg gggcgtcaca ggttgatatg acgctggccc acaccggtta
     6781 caccgcgggt caggtgttac gtgcctggac accgttcctg ttcctgaccg ctaccgtaac
     6841 actgtggagc atcccgccgt ttaaagccct gttcgcatcg ggtggtgcgc tgtatgagtg
     6901 ggtgatcaac attccggtgc cgtacctcaa taaactggtt gcccgtatgc caccagtggt
     6961 cagcgaggct acagcctatg ccgccgtgtt taagtttgac tggttctctg ccaccggcac
     7021 cgccattctg tttgctgcac tgctctcgat tgtctggctg aagatgaaac cgtctgacgc
     7081 tatcagcacc ttcggcagca cgctgaaaga actggctcta cccatctact ccatcggcat
     7141 ggtgctggcg ttcgccttta tctcgaacta ttccggactg tcatcaacac tggcactggc
     7201 actggcgcac accggtcatg cattcacctt cttctcgccg ttcctcggct ggctgggggt
     7261 attcctgacc gggtcggata cctcatctaa cgccctgttc gccgcgctgc aagccaccgc
     7321 agcacaacaa attggcgtct ctgatctgtt gctggttgcc gccaatacca ccggtggcgt
     7381 caccggtaag atgatctccc cgcaatctat cgctatcgcc tgtgcggcgg taggcctggt
     7441 gggcaaagag tctgatttgt tccgctttac tgtcaaacac agcctgatct tcacctgtat
     7501 ggtgggcgtg atcaccacgc ttcaggctta tgtcttaacg tggatgattc cttaatgatt
     7561 gttttaccca gacgcctgtc agacgaggtt gccgatcgtg tgcgggcgct gattgatgaa
     7621 aaaaacctgg aagcgggcat gaagttgccc gctgagcgcc aactggcgat gcaactcggc
     7681 gtatcacgta attcactacg cgaggcgctg gcaaaactgg tgagcgaagg cgtgctgctc
     7741 agtcgacgcg gcggcgggac gtttattcgc tggcgtcatg acacatggtc ggagcaaaac
     7801 atcgtccagc cgctaaaaac gctgatggcc gatgatccgg attacagttt cgatattctg
     7861 gaagcccgct acgccattga agccagcacc gcatggcatg cggcaatgcg cgccacacct
     7921 ggcgacaaag aaaagattca gctttgcttt gaagcaacgc taagtgaaga cccggatatc
     7981 gcctcacaag cggacgttcg ttttcatctg gcgattgccg aagcctcaca taacatcgtg
     8041 ctgctgcaaa ccatgcgcgg tttcttcgat gtcctgcaat cctcagtgaa gcatagccgt
     8101 cagcggatgt atctggtgcc accggttttt tcacaactga ccgaacaaca tcaggctgtc
     8161 attgacgcca tttttgctgg tgatgccgac ggggcgcgta aagcaatgat ggcgcacctt
     8221 agttttgttc acaccaccat gaaacgattc gatgaagatc aggctcgcca cgcacggatt
     8281 acccgcctgc ccggtgagca taatgagcat tcgagggaga aaaacgcatg attatttccg
     8341 cagccagcga ttatcgcgcc gcagcgcaac gcattctgcc gccgttcctg ttccactata
     8401 tggatggtgg tgcatattct gaatacacgc tgcgccgcaa cgtggaagat ttgtcagaag
     8461 tggcactgcg ccagcgtatt ctgaaaaaca tgtccgactt aagcctggaa acgacgctgt
     8521 ttaatgagaa attgtcgatg ccggtagcac tggcaccggt gggtttgtgt ggcatgtatg
     8581 cgcgtcgtgg cgaagttcag gcagccaaag cggcggacgc gcatggcatt ccgtttactc
     8641 tctcgacggt ttccgtttgc ccgattgaag aagtcgcgcc agccatcaag cgcccaatgt
     8701 ggttccagct ttatgtactg cgcgatcgcg gctttatgcg taacgcgctg gagcgagcaa
     8761 aagcagcggg ttgttcgacg ctggttttca ccgtggatat gccgacaccg ggcgcacgct
     8821 accgtgatgc gcattcaggt atgagcggcc caaatgcggc aatgcgccgc tacttgcagg
     8881 cggtgacgca tccgcaatgg gcgtgggatg tgggcctgaa cggtcgtcca catgatttag
     8941 gtaatatctc agcttatctc ggcaaaccga ccggactgga agattacatc ggctggctgg
     9001 ggaataactt cgatccgtcc atctcatgga aagaccttga gtggatccgc gatttctggg
     9061 atggcccgat ggtgatcaaa gggatcctcg atccggaaga tgcgcgcgat gcagtacgtt
     9121 ttggtgctga tggaattgtg gtttctaacc acggtggccg ccagctggac ggtgtactct
     9181 cttccgctcg tgcgttgccc gctattgcag atgcggtgaa aggtgatatc gccattctgg
     9241 cggatagcgg aattcgtaac gggcttgatg tcgtgcgtat gattgcgctc ggtgccgaca
     9301 ccgtactgct gggtcgtgct ttcctgtatg cgctggcaac agcgggccag gcgggtgtag
     9361 ctaacctgct aaatctgatc gaaaaagaga tgaaagtggc gatgacgctg actggcgcga
     9421 aatcgatcag tgaaattacg caagattcgc tggtgcaggg gctgggtaaa gagttgccta
     9481 cggcactggc tccgatggcg aaagggaatg cggcatagtc gtttgcgccc cctcacccta
     9541 accctctccc tcagggagag gggaccgttc ggcgctgtat gtactccctc actctgaaac
     9601 gacaccgcac tctttttttc tccctcgccc ctccggggag ggccggggtg aggggaaaag
     9661 gccgcacggt tcaccaattt tctgctatcc tgcccccgca ctaagggggc agtatgctaa
     9721 acatcgtact ttacgaacca gaaattccgc caaatactgg caacatcatc cgtctttgcg
     9781 ctaataccgg ctttcgtcta catatcatcg aaccgatggg atttgcctgg gacgacaagc
     9841 gcctgcgccg cgcggggctg gactatcacg agtttaccgc cgttacgcgt catcatgact
     9901 atcgcgcgtt cctcgaagca gaaaatcccc agcgcctgtt tgccctcacc acgaaaggta
     9961 cgcctgctca cagcgccgta agctatcagg atggcgatta tctgatgttc ggcccggaaa
    10021 cacgcggcct gccagcgagc attcttgatg ccctgcccgc tgaacaaaaa attcgcattc
    10081 cgatggtgcc ggacagccgc agcatgaatc tgtccaatgc ggtgtcggta gtggtgtatg
    10141 aagcctggcg gcagctggga tatccgggag cgttgttgag agattagtta ctgtatgccg
    10201 gatagatgat tacatcgcat ccggcacgat cacaggacat tagatcccat ccccatactc
    10261 aaatgtatgg ttaataccgt tgaaatgctg gtccatatcc attgatggct tatcgctgtc
    10321 tggtttaccg acaatacgag ccggaacgcc agcggcggtt gtatgcggcg gcaccggttg
    10381 cagcaccacg gaacctgcgc caatcttcgc gccgcaacca acttcaatat tgccgaggat
    10441 tttcgcgccc gcgccaatca tcacaccttc acgaattttc gggtgacggt caccaccaga
    10501 tttacccgta ccgccaagcg tcacagattg cagaatcgat acgtcgtttt caattaccgc
    10561 cgtttcaccc acaacgatgc ccgtggcgtg gtcgagcata atgccacggc caatttttgc
    10621 tgccgggtga atatcgacct ggaacgtcac agaaacctgg ttttgcagaa agattgccaa
    10681 tgcgcgacgc ccctgattcc acaaccagtg accgatgcga taggcctgca aggcatgaaa
    10741 acccttcagg tataacaacg gggttgagta tttatcgact gccgggtcgc gggtacgcac
    10801 cgcctgaata tcacaggccg cagaggcgat catttccggg tcagcggcgt aggcttcttc
    10861 aaccacttca cggatagcaa tagcaggcat aattggcgat gacagcttgt tcgccagcat
    10921 gtagctcagt gcactgccaa ggttttcgtg cttgagtagc gtcgcgtggt aaaaactggc
    10981 cagcattggc tcacagtccg ccagcgttct ggcttcggct ttaatattgt tccagacaat
    11041 ttccagttct tcacacgaca ttgcttactc cacacgatga gataatgacc ggcccgttct
    11101 gcgcgggccg ggtcatagcg gtaacaaagg ttccctgggg ttagtgactg ctgcgctcgt
    11161 ccttgcgtgc acgacctaat aatgtcaatg ctgcctcgcg cgcgtttttt ccgcaatata
    11221 atacttgata aatttcctcg gttattggca tttcaacgcc gaagcgatgc gccagttcgc
    11281 ggacttcttt cgtattgcgg tagccttcca ccacctgacc aatcttctcc tgcgcgcttt
    11341 gtacatccat gccctgaccg agcatcatac caaaacggcg gttacgcgac tggttgtcgg
    11401 tacaggtaag caccagatcg cccagccccg ccatgcccat aaaggtggca ggatcggcac
    11461 ccagcgccgc accaagacgc gacatttcag ccagcccacg ggtgatcagc gccgtacgcg
    11521 cattcgcacc aaaaccgata ccgtcagaca tccccgcgcc aatggcaatg acgtttttca
    11581 ccgcgccgcc aagctgcacg ccaatgaaat ccgggttgct gtaaacgcgg aaacttttgc
    11641 cgcagtgcag cagctgctgg agatcatcgg caaaggtctg atcggtcgag gccagcgaaa
    11701 tagctgtcgg taaacctgcc gccagttctt tcgcaaacgt tgggccagag ataaccgcca
    11761 gcggaatttg atcgcctaag gcctcacgcg ccacgtcctg taacagacgt ccggtttccg
    11821 cttccagccc tttggtcgcc cacaccagac gcgcatcagg acgcatcagc ggtttaatct
    11881 ggcgcagcac ttcaccaaag acatggctgg gtacgacgac gagaatatta cgactggctg
    11941 ccagcgcagt ggcgagatcg ctttcaagat ggagcgtatc gggaaaaggc acatcgggga
    12001 gaaacgcggc gttacagcgg tcgcgttcaa gcgttgcgat atgttcaggg tcatggcccc
    12061 agaggacaac ctcgtggcca tttcttgcca gggtaatggc aagagcggtg ccgtacgagc
    12121 cggcaccgat cacagtcatt gaagcattac gttggttcat caggcatcct gatgttcttc
    12181 agtaccttcg ccagcctgct gctgcaaata gttcatgaac agcgcatcga agttaaccgg
    12241 cgcaaggttc agttgcggga atgtaccgcg ggataccatg ctggtgatgc actcacgagc
    12301 atacgggaac agaatgttcg ggcagtatgc gcccaggcaa tgtgccatct gggtgccttc
    12361 gatacccgcg atggagaaaa taccgccctg ctgaacttca cacaggaacg cggtttcttc
    12421 gcccaaggag gccgttacgg taacacgcag taccacttcg tatacgtcat ctgccagttg
    12481 ggaagatgcc gtatccagat caagtttaac ttctggttgc caatctttct ggaaaacgtg
    12541 cggcgcgttc ggcgcttcga aagagatatc cttggtataa atacgttgga tctggaaagt
    12601 catttcagtg ttgttttgtt ctgacatgtg tagaaaaccc ttaagtgttg tccttaaata
    12661 ctgcgcaatg taataccaac gttcacacgt tatttcagca ggggatccag tccaccacgt
    12721 gcatccaatg catacaagtc atcacagccg ccaatgtgct gtgcgtcaat aaaaatctgg
    12781 ggaaccgtgg tgcaaccgct gcgtttgatc atctcttcac gcttggcggc attgccatcg
    12841 atcggcaact cctggaaact cacgcccttg ctgctcagca gtgcttttgc acgatggcaa
    12901 tacgggcagg tttctttggt atagatttca acattggcca taatttagct cctgtttttt
    12961 taccctgaaa tttcatgttg cagggcggct gcaaaatgga gtgtccccag aagcttataa
    13021 caataagtcg ctgggggcct aattgcagct aacggcctgc atcatgaaag acgacaggta
    13081 aattatttgc cgcgcaccag aggcaagttt tcgccagccc agccagcaac gccctctttc
    13141 agcacgaata cttgcgcaaa accagctttc gtcagcgcgt ttgcaggctc ctggcactgc
    13201 atgccagaac cgtctaccac gataaccgat ttgtctttgt gcttctcaag ctcaccaaca
    13261 ttgttggctt tgatttcgct cggcaacaga ttgatagaac ctgcgatatg gcctttacgg
    13321 aagtcatcac gctgacgtaa atccacaacc acagcgtctt ctttgttgat cagacgcgta
    13381 gcttcaccac gagtaatcac cttcactttc gaggtcaggc tcttaaacgt agtcacaaga
    13441 accgccacca gtaacgcgat ccaggcgata ctcagtatgg gatgacggcc aacaaattgc
    13501 ataatttctt gcatgggggg taacaactcc cgacgtagtg attaaaaaaa ccaggaaagg
    13561 agtatacctg cgcggtgcgg caaatacagc cagcgcgtta actggaatgc aattttgcgg
    13621 ggcacgacga aaaaaaagcg caaatctggc atcggcttgc cgcgagcggt cgtatatttt
    13681 gatcttcaga ggctatttta tcgattcagc tgtagtaaaa ttacgcaaat tttgactctt
    13741 gagtatgagg ttgtcgcaat gtcggtttct aaaaaaccta tggtactggt gattctggat
    13801 ggctatggct atcgtgaaga acagcaggat aacgccattt ttagtgctaa aaccccggta
    13861 atggatgcac tgtgggccaa tcgtccgcat accctgatcg acgcttccgg tctggaagtc
    13921 ggtctgcctg accgtcagat gggtaactcc gaagtaggcc acgttaacct gggtgccggc
    13981 cgcatcgtgt atcaggacct gactcgtctg gacgttgaaa tcaaagatcg cgctttcttt
    14041 gctaatccag tgctggctgg cgcggtacat aaagcgaaaa acgcaggtaa agcagtacac
    14101 attatgggtc tgctctccgc aggcggcgta cacagccacg aagatcacat catggcgatg
    14161 gtagaactgg cagctgaacg cggcgcagaa aaaatctacc tgcacgcatt ccttgacggt
    14221 cgcgacaccc cgccgcgcag tgctgaatcc tcgctgaaaa aatttgaaga gaaatttgct
    14281 gcgctgggta aaggccgcgt agcgtccatc attggtcgtt actacgcgat ggaccgcgat
    14341 aaccgttggg atcgcgttga aaaagcctat gacctgctga ctctggcgca gggtgagttc
    14401 caggccgata ctgccgttgc tggtttgcag gcagcttatg ctcgcgacga aaacgacgag
    14461 ttcgtgaaag cgaccgttat tcgtgcagaa ggtcagccag atgcggcaat ggaagacggc
    14521 gatgcgctga ttttcatgaa cttccgtgct gaccgcgcgc gtgaaatcac tcgtgcattc
    14581 gtgaacgctg atttcgatgg cttcgcgcgt aagaaagtgg ttaacgtcga tttcgtgatg
    14641 ctgaccgaat acgccgctga catcaaaact gcggttgctt acccacccgc ttccctggtt
    14701 aacaccttcg gcgagtggat ggcgaaaaac gacaaaactc agttgcgtat ttccgaaacc
    14761 gaaaaatatg cccacgttac tttcttcttc aacggtggcg tagaagagtc gttcaaaggc
    14821 gaagatcgca ttctgatcaa ctcaccgaaa gtggctacct acgatctgca accggaaatg
    14881 agctccgcag agctgaccga aaaactggtt gcggccatca agagcggcaa atacgacacc
    14941 attatctgta actatccgaa cggcgacatg gtaggtcaca ccggggtgat ggaagcggcg
    15001 gttaaagcgg ttgaagcact ggatcactgc gtggaagaag tcgcgaaagc ggttgagtcc
    15061 gtgggtggac aactgctgat caccgctgac cacggtaacg ctgaacagat gcgcgatccg
    15121 gcaacgggtc aggcacacac ggcacacacc aacctgccag ttccgctgat ttacgttggt
    15181 gataagaacg tgaaagcggt tgcaggcggc aaactttctg acatcgcgcc gaccatgttg
    15241 tcgctgatgg gtatggaaat cccgcaagag atgactggta agccgctgtt catcgtggaa
    15301 taatccctcc ccatgagggg aaaggcgatt aataccatga cacgggccgt gaaaccgcgc
    15361 aggtttgcaa tcaggcccat catctacgcc agcgttctta gcgctggcgt attgttgtgc
    15421 gccttttccg cccacgcgga tgagcgtgac caactcaaat ctattcaggc cgatatcgcc
    15481 gcaaaagagc gcgcggtacg ccaaaagcaa caacaacgcg caagcctgct cgcacaattg
    15541 aaaaagcagg aagaagcgat ctctgaagcc acccgtaagc tgcgcgaaac gcaaaacacg
    15601 ctcaatcaac tcaataaaca gattgatgag atgaacgcgt cgattgccaa actggagcag
    15661 caaaaagccg cccaggagcg cagcctcgcc gcacaactgg atgccgcatt ccgtcagggc
    15721 gagcataccg gtattcagct aattctcagc ggtgaagaaa gccagcgtgg acagcgttta
    15781 caggcttatt tcggctatct caaccaggcg cgacaagaaa ccattgccca gttgaagcaa
    15841 acgcgtgaag aagtcaccat gcagcatgct gaactggaag agaaacaaag cgagcaacaa
    15901 acgctgttat atgaacagcg cgcccaacag gcgaaactga ctcaggctct gaacgagcgt
    15961 aaaaagacgc tggcggggct ggaatcttcc atccagcaag gtcagcagca gctaagtgaa
    16021 ctgcgtgcca acgaatcccg tctgcgtaac agcattgccc gtgctgaagc cgcggcgaaa
    16081 gcgcgtgctg aacgagaagc gcgtgaggcc caggcggttc gcgagcgcca gaaagaagcg
    16141 acgcgcaaag gcaccaccta caaaccgacc gaaagcgaaa aatcgctgat gtcccgaact
    16201 ggtggcctgg gtgcgccgcg tggtcaggca ttctggccgg ttcgcgggcc gacgctgcat
    16261 cgctatggcg agcagctaca gggtgaatta cgctggaaag ggatggttat cggtgcctct
    16321 gaaggtactg aagttaaagc aattgccgac ggtcgagtga ttctggctga ctggctgcaa
    16381 ggttacggtc tggtggtggt ggttgagcac ggtaaaggcg acatgagtct ttacggctat
    16441 aatcagagcg cactggtgag cgttggttcg caggttcgcg cgggccagcc aattgcactg
    16501 gtgggcagca gtggcggtca gggtcggcct tcactctatt tcgaaattcg ccgccagggt
    16561 caggcggtca atccacagcc gtggttggga agataagttt tgtttccatt tcgtcgtaac
    16621 gttcttgcat ttgccgctct gttggcgctc tcctcccccg tacttgctgg caaacttgcc
    16681 atcgtcattg atgattttgg gtatcgcccg cacaacgaaa accaggtgct ggcgatgcct
    16741 tccgctatct ccgtcgctgt attacccgat tcaccgcacg ccagagagat ggcgaccaaa
    16801 gcgcataaca gcggtcacga agtgttgatt catctcccga tggcaccgtt gagtaaacag
    16861 ccgctggaga aaaatacgct acgcccggag atgagcagcg acgaaattga gcgcattatt
    16921 cgtagtgcgg tcaataacgt gccctatgcc gtggggatca acaaccacat gggcagcaag
    16981 atgacctcta acctgtttgg tatgcagaaa gtgatgcagg cgctggagcg ttacaatctt
    17041 tacttcctcg acagcgtaac catcggtaat acccaggcga tgcgcgccgc gcaaggtact
    17101 ggcgtgaagg tgatcaaacg gaaggtgttc ctcgacgatt cgcagaacga agcggacatc
    17161 cgtgtgcaat ttaatcgcgc aattgatctg gcgcgtcgca acggttcaac cattgccatt
    17221 gggcatcctc acccttcaac ggtacgcgtg ctacaacaga tggtttataa cctgccgcca
    17281 gacattacgc tggtgaaagc cagcagcttg ctgaatgaac cgcaggttga tacttctaca
    17341 ccgccgaaaa acgctgtgcc tgatgcaccg cgtaatccgt tccgtggcgt gaagctgtgc
    17401 aaaccgaaga aaccgataga acctgtttat gctaatcgct tctttgaagt attaagcgaa
    17461 agcatcagcc agagcacgct gatcgtttac ttccagcatc agtggcaagg ctggggcaaa
    17521 cagcccgaag cggcgaagtt taacgctagc gcaaattaaa ggcgctacgc gtaatgcgat
    17581 ggtgcgaccg cgttttgtcg cgccattgcc ataagcggaa agaccacagt aacgcctggt
    17641 aaccgacctt cacgctgcgc acattggtaa tcaggcgctt atacataccc ggaagtaaat
    17701 atctcggcaa tcatccgttg gcgggtaaga atatccggct ctttgcg
//
LOCUS       DAZOYF010000076        17602 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZOYF010000076 DAZOYF010000000
VERSION     DAZOYF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.076"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACHUOF_002391"
                     /pseudo
     CDS             complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACHUOF_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACHUOF_002392"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACHUOF_002392"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR0611540.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="ACHUOF_002393"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="ACHUOF_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="ACHUOF_002394"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="ACHUOF_002394"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR0611541.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="ACHUOF_002395"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="ACHUOF_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR0611542.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="ACHUOF_002396"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="ACHUOF_002396"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR0611543.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="ACHUOF_002397"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="ACHUOF_002397"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR0611544.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="ACHUOF_002398"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="ACHUOF_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR0611545.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="ACHUOF_002399"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="ACHUOF_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR0611546.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..8145)
                     /gene="astE"
                     /locus_tag="ACHUOF_002400"
     CDS             complement(7177..8145)
                     /gene="astE"
                     /locus_tag="ACHUOF_002400"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR0611547.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8149..9470)
                     /gene="astB"
                     /locus_tag="ACHUOF_002401"
                     /pseudo
     CDS             complement(8149..9470)
                     /gene="astB"
                     /locus_tag="ACHUOF_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9467..10945)
                     /gene="astD"
                     /locus_tag="ACHUOF_002402"
     CDS             complement(9467..10945)
                     /gene="astD"
                     /locus_tag="ACHUOF_002402"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFR0611548.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10942..11976)
                     /gene="astA"
                     /locus_tag="ACHUOF_002403"
     CDS             complement(10942..11976)
                     /gene="astA"
                     /locus_tag="ACHUOF_002403"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR0611549.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11973..13193)
                     /gene="astC"
                     /locus_tag="ACHUOF_002404"
                     /pseudo
     CDS             complement(11973..13193)
                     /gene="astC"
                     /locus_tag="ACHUOF_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13639..14445
                     /gene="xthA"
                     /locus_tag="ACHUOF_002405"
     CDS             13639..14445
                     /gene="xthA"
                     /locus_tag="ACHUOF_002405"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR0611550.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14612..15322
                     /locus_tag="ACHUOF_002406"
     CDS             14612..15322
                     /locus_tag="ACHUOF_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0611551.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15327..16004
                     /gene="ydjY"
                     /locus_tag="ACHUOF_002407"
     CDS             15327..16004
                     /gene="ydjY"
                     /locus_tag="ACHUOF_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR0611552.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16019..16726
                     /locus_tag="ACHUOF_002408"
     CDS             16019..16726
                     /locus_tag="ACHUOF_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0611553.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16726..17274
                     /locus_tag="ACHUOF_002409"
     CDS             16726..17274
                     /locus_tag="ACHUOF_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR0611554.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17284..>17602
                     /locus_tag="ACHUOF_002410"
     CDS             17284..>17602
                     /locus_tag="ACHUOF_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0611555.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRT"
BASE COUNT         4350 a         4678 c         4299 g         4275 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatactc tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tacggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttgacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctcgcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatccgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattct acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgagt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acacgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca acacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgccg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgccgtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc ctccctcgcg
     8161 ctggaacgga taaaccgaac cgagattcag taattggctc aatacatcca gtgcttcccg
     8221 cccttcgcgc agcaattgcg ggtcggccag atcggcagca gtaagacgat cgcggtagta
     8281 acgatccacc cagtcattga gcgcattaaa caaggtatcg ttcatcatca ccgccggatt
     8341 caccgcccgg cgttcttctt ctgtcaatac cacccgcaac cgcaggcacg ccgggccgcc
     8401 gccattcgcc atgctttcac gtaagtcaaa gacttttagt tcgctaatcg ggttgtcagc
     8461 ggcaaggagt tcattgagat aaccccatac tccggcgtgt tcccgacact cctgaggcag
     8521 caccaacatc atggaaccat catcgcggct caacagttgg ctgttaaaca gataggtaga
     8581 caccgcatcc gacacggaaa cctgagttgc cggaacttct atcgccataa aaccgttcac
     8641 ccgcgtgcca gtaactgtga ctggcgagcg aacgcctgtt ggtggcaaaa cagcacctgg
     8701 cggttactca cggcaatcac gtcattatga aaaacgctct ggtcgataac gtccgggttt
     8761 tgctgggcga aaatcacctg ttggggattc acctgattaa gccttgccac cgcctcgctg
     8821 gcttcgcgag tctgtcgcgc cggataacgg gaaggccggg tatcattgcc ttcttctcgc
     8881 ccgtagacaa aaagtagcat acccggttca ccgtaatgac cgccgagacg attgtggttt
     8941 gccgccccct catcaccgag caacgctacc tgcggcaacg ccgaatggac gctaaatttc
     9001 tcttcgtcct taaaaatcgc ttttaacagc gattcagtga cgggcgcttc cagcgaacgg
     9061 tgaaatttat tgttcaggtt ggcaacggtg agatgcactt tgccatccag cgtatcggca
     9121 gatggcgcga tcgttgccgc attggctacc cacattggcg aagcggaact gacgctggaa
     9181 agccagtgcg gtgcctggcg tgcaactttt tccagtacct gctcatcgct accactgaat
     9241 cccaactgac gcagcaccgg aataaacgga cgctcgtgcg gcggaatcac ggcctggggg
     9301 aatcccgcat cggcaagggc tttcattttc agtaagccct gttttgccgc cagtcgcggg
     9361 ttagacacct gaaaacggtg acgggtagag gcttcattac caaacgacag gcccgcgtaa
     9421 tgatgcgtca gccctaccag cccgtcgaaa ttaacttccc aggcgttcat cgcaccacct
     9481 catcggaaaa atccagcccg gggttaagcg tggcgggcaa tgttaacgag tccgactcca
     9541 ggctcgccat aggccatgcg cagtaatctg cggcatacca ggcgctgggg cgatggttac
     9601 cggaagcacc aatgccgccg aatggggcgg tactggcagc accagtaagc ggtttgttcc
     9661 agttaacaat ccccgcccgc gcctccagca acagttgatc gaatttttcc cgctcggggg
     9721 aaaccagacc gcaaaagagt ccgaagcgag tgttgttcgc catccgaatc gcctcatcaa
     9781 aagtatcata acgccagacg cgcaaaaacg gcccgaacac ctcttcatcc ggcacaccgc
     9841 caacgcccgt catttcaatg atccctggtg tcagcaacga tgtccctgct tgtaataagc
     9901 gcggcgcaag cagggttcgt ccgcccatcg cttccagttg ctgccaggca gtaaccacct
     9961 gctgtgcggc ctgttcagaa atcagcccgc caataaacgg ctgcggttcg tcatcccagt
    10021 tgcccggcgt taatcgctgg ctgacggcaa ccagactagc aagaaacgca tcgccctgcg
    10081 ctccgctttt gagcaataaa cggcgggcgc aggtgcagcg ttgcccggct gtgacaaacg
    10141 ccgactgaat ggtcagatgg acagccgcgt cgatatccgc cacctcatcg ataattagcg
    10201 gattattacc gcccatctca agggcgagaa ttttctccgg ctgaccggag agctggcgat
    10261 gcagctggta gccggtattg gcgctaccgg taaacagcaa accgtcgaga tcctccagcg
    10321 cactcagcgc ctgacccgtt tcacgcccgc cctgcaccag gttcagcacg cccggcggca
    10381 agccagcctg ctgccataaa cgcattaccg cttcgccact ccacggtgtc agttcgctgg
    10441 gtttaaagat aatggtgtta cctgccagca atgccggaac gatatgtccg ttcggcaaat
    10501 gaccagggaa attatacggc ccaaacaccg ccagcacgcc gtgcgggcga tgtcgcaggc
    10561 tcgccgcacc gtccggcatt tcactacgct gctcgccggt acgaacgtga tacgccttaa
    10621 ttgatatcgc gattttattg atcatcgccg tcacttcggt tgccgcttcc cagcgcggct
    10681 tacccgtttc tctggcaata atcgcggtta attcggcttt attgctttcc agcaatccgg
    10741 caaagcgttc gacaacgacc tgacgttcag ccaatgagag ccgcgcccag cgcggaaacg
    10801 ccgcacgggc tgcccgacaa gcctgttcga cctgagcggc atcggcatca ttgccttgcc
    10861 ataacacctc gcccgatacc ggattacgct tcacacgcga tgcgccctgg cccgttatcc
    10921 agtcaccgtt aatccataaa gtcatgctgt tttctcctct gcgcacaggc gcaccagacg
    10981 aacgcgatcc cctgcgtggc atttgagggc atccagttgt gcggcggtta aaatcaaacg
    11041 ctcggttgcc ggatcggtac gcgccagcac cacgcggaaa tggtgataat tttcattggc
    11101 gaccaggcag gccggaaaat cgccctgcgc aggctgccct tctgccactt ccaccagccg
    11161 acttttacgg atggcgcgca cgcggtcgat gtcacactca agcgttggcc cgccgtcgaa
    11221 gatgtcgata tagttgcggt aacgaaaacc ttctttctcc agcaccgcgc gggcaggcgc
    11281 ggtttgcgga tgtacctgac cgatgacgtc ctgggcttcc tgggataaaa agtgggtata
    11341 gatcggatgt ttcggcatca gttctgcaat aaatgccttt tgcccggtgc cgcagagaaa
    11401 atcggcgcgg ctaaaatcca tcgaaaagaa gcgtttaccg aggctttgcc agaacggtga
    11461 atagccgtgt tcgtcaatca ccccgcgcat ttcagcaacc actttgtcat taaacttgtc
    11521 gcgaaaagcc gccataaaca taaagcgcga tttcgacagc aaatagccgt tgccctcttt
    11581 gcgccagtcc gggtcgagaa acagcgtgca cagctcgctg ctgccggtgt gatcgttact
    11641 aagaaacagc gtcggcaatg cgttatagac attcagctct tttgaggcgt gaaccaacgt
    11701 gccgacgcga tagttgtacc agggatcgtt cagcccaacc gccacctcaa tagcgcaaat
    11761 ccccgccacg gtgcctgtct cgctatcttc cagcacgaac acatagccct gctcgctttt
    11821 gggcagttca ccttgccagg ttttgattgc cctttcgata cgcgccgaaa gcgtggcttc
    11881 attggcggga agcgacgtca ggccgccgcc cgttttgctg gcaagctgca tcagcgccga
    11941 gacatctgag cgctcaacgg gacggatgac catcatgatg aacctcggct aacaaagtgt
    12001 tcgcaagcag ctgcaaagcg atccagtccg gtcgtcacct cttcttcgct gacattgagc
    12061 gcaggcgcaa aacgcaccac gttgccaccc gcaatcagta ccatcacgcc tgctttcgcc
    12121 gcttccagag agatctgttt cgcttgcccg gcgtaatcgg cattcagtac acagccaatc
    12181 aacaaaccta agccgcgaac ttcactgaac aaaccatagc ggtgattaat agtattaaga
    12241 cgctcaacaa accagtcgtg acgctgttta acgccattaa gcatctctgg tgtgttgatg
    12301 agctccagca ctttgcctgc taccgccgag gccagcggat taccgccata ggtggtgcca
    12361 tgagtgccaa cggtcatcac gcgggcgcac tcttcggttg ccaacaacgc accgaccggg
    12421 aaaccgccgc ccagcgcttt ggcggtggtt aacagatcgg gcgtcacgcc gtagtgcata
    12481 taggcataca gctcaccagt gcgcccgacg ccggtttgta cctcatcaaa aatcagcaag
    12541 gcattgtggc ggtcacacaa ttcacgcaga ccttgtaaaa acgcgttgct ggctggcacc
    12601 acaccgcctt cgccctagat gggctcgaca atcaccgcac aggtggcgtc gtcaatcagc
    12661 gcgctggcag agttaatatc gttatatgca gcatgacgaa tatccgccgg cagtggcgca
    12721 aaatcctgtg aataggctgg ctgcccaccc gcactgacag taaacagcgt gcgaccatga
    12781 aacgcatttt tgaacgccac gatgccactc ttatggctgc cgtagcggtc gtgagcgaat
    12841 ttacgcgcca gttttagcgc cgcttcgttg gcttccgcac cggagttaca aaagaagacg
    12901 cgatcggcaa acgtggcgtc gatcaatttt ttcgccagtc gcagtaccgg ctcgttggtg
    12961 taaccgttgc cggtatgcca gaacttactc gcctgttcgt tcagcgcttc acgcagttcc
    13021 ggatgcgcat ggcccagcgc gttcaccgca atgccacccg cgaagtcgat atactctttc
    13081 ccctgctgat cccacaagcg cgaaccttcg ccacgtaccg gtataaaggg tgccggagcg
    13141 taaacaggta tcatccattc atcaaagttt tcacgcgtaa ttggctgaga catagcgacc
    13201 tctacagtaa ataattcgtt atttgtatgt taacaataag taatgtttgc gctgtaaatg
    13261 tagattgcag ggttcgtgcc agccagtgat aaaagtgcat aaacggcgga ggctaactgg
    13321 aaatcaagga gttataacca aaccatatgc atttaaagtg catataaagt gaatacgttt
    13381 gcgatgtggg tgaataaaaa gaataaaaaa cgcaatgtta tgcagaagta aaatataatt
    13441 ctagaattgt gatcattgac gaaatttact ggaaattact gcgccattct gacgcagcgc
    13501 gcaccaaaag cgggcatttt ttgcgccatc gttgacatca ttaacaacca tcgatcaaat
    13561 cacttaacaa caggcggtaa gcaacgcgaa attctgctac catccacgca ctctttatct
    13621 gaataaatgg cagcgactat gaaatttgtc tcttttaata tcaacggcct gcgcgccaga
    13681 cctcaccagc ttgaagccat cgtcgaaaag caccaaccgg atgtgattgg cctgcaggag
    13741 acaaaagttc atgacgatat gtttccgctc gaagaggtgg cgaagctcgg ctacaacgtg
    13801 ttttatcacg ggcagaaagg ccattatggc gtggcgctgc tgaccaaaga gacgccgatt
    13861 gccgtgcgtc gcggctttcc cggtgacgac gaagaggcgc agcggcggat tattatggcg
    13921 gaaatcccct cactgctggg taatgtcacc gtgatcaacg gttacttccc gcagggtgaa
    13981 agccgcgacc atccgataaa attcccggca aaagcgcagt tttatcagaa tctgcaaaac
    14041 tacctggaaa ccgaactcaa acgtgataat ccggtactga ttatgggcga tatgaatatc
    14101 agccctacag atctggatat cggcattggc gaagaaaacc gtaagcgctg gctgcgtacc
    14161 ggtaaatgct ctttcctgcc ggaagagcgc gaatggatgg acaggctgat gagctggggg
    14221 gtggtcgata ccttccgcca tgctaatccg caaacagcag atcgtttctc atggtttgat
    14281 taccgctcaa aaggttttga cgataaccgt ggtctgcgca tcgacctgct gctcgccagc
    14341 caaccgctgg cagaatgttg cgtagaaacc ggcatcgact atgaaattcg cagcatggaa
    14401 aaaccgtccg atcacgcccc cgtctgggcg accttccgcc gctaatttag cagctctcct
    14461 ggctcaaact gggtcaggag aattaacctt gagaaaaatc aacaaactgt cagtaatgat
    14521 ttgttgcctg ccgtcctttg ttataccgtc actgcgtttt tagttgtctg accacttctc
    14581 tattatcaag tttgatatag gaaactccac gatgaacgct gagcgtaact ttctgtttgc
    14641 ctgtcttatt tttgcgctgg tcatttacgc tatccacgct ttcggtttat tcgatctgct
    14701 caccgattta ccccacttac agacactcat tcgccagagc ggattgttcg gttatagcct
    14761 ctatattctg ttattcatca atgcccccct ctttctgtta ccaggaagca tactggtgat
    14821 cgctggagga atagtttttg gcccgctctt agggacacaa ctctcattaa ttgccgcaac
    14881 gctggcctcc tcgtgctcat tcctgctggc gcgctggctg ggacgtgatt tactgctgaa
    14941 atacgtgggc catagccata ccttccaggc cattgaaaaa ggcattgcgc gtaacggtat
    15001 tgattttctt attctgaccc gcttaatccc gttgtttcct tacaatattc aaaattacgc
    15061 ttacggattc accaccatcg ccttctggcc ttataccctt atttcggcac tcacgaccct
    15121 gcccggtatt gttatttata ccgtgatggc aagcgatctc gccaatgaag gcattacgct
    15181 gcgctttatt ttacaactct gtctggcggg cctggcgctg tttattctca tccagctcgc
    15241 aaaactctac gcccgacaca aacatgtgga tctgtctgct tcgcgccgca gcccacttac
    15301 tcacccaaaa aatgaaggat agaacgatgt cgcaacatta ttcagtgtca tggaaaaaag
    15361 gactggctgc actctgctta ctggctgttg ccgggcttag cggctgcgat caaaaagaga
    15421 atgcggcggc aaaagtggaa tacgacggac tttcgaacag ccaaccgctg cgcgtcgatg
    15481 ccaataacca tacggtaacc atgctggtgc aaattaatgg tcgtttcctc accgacgaca
    15541 ctcgtcacgg tattgtgttt aaagatggct ccaacggaca taaatcgctg tttatggctt
    15601 atgcgccccc gaaagcattt tatgaagccc tgaaagaggc aggtggtacg ccgggcgaaa
    15661 acatgacgat ggataataaa gaaacgactc atgtcacagg cagcaaactg gatatttcgg
    15721 tcaactggca aggggcggca aaagcgtatt ccttcgatga agtgattgtt gacagtaatg
    15781 gcaagaaact ggacatgcgc tttggcggta atttaacggc agcagaagag aagaaaacgg
    15841 gttgcctggt gtgtctggat agctgcccgg tcggcatcgt cagcaatgca acatacactt
    15901 atggtgcggt ggaaaaacac ggcgaagtga aattcaaagg caatgcctca gttctcccgg
    15961 cggataacac gctggcaacg gttaccttta aaatcaccga ataaagccaa gataaaggat
    16021 gatgaagata caatcgcgta aaatctggta ctaccgtata accctcatca tcctgttgtt
    16081 cgctatgctg ctcgcatggg cgctgcttcc cggcgtccat gagtttatca atcgcagcgt
    16141 tgcggcgttt gccgccgtgg accaacaggg tatagaacgc tttattcagt cttacggcgc
    16201 actggcggca gttgtctcgt tcttgttgat gattttacag gccattgctg caccgctacc
    16261 tgcgtttttg atcacctttg ccaatgcgtc gctgtttggc gcgttctggg gcggcttgct
    16321 gtcgtggacc agttcgatgg ccggcgcggc gctgtgcttt tttatcgcca gagtgatggg
    16381 ccgcgaagtg gtggaaaaat taaccggcaa aaccgtgctt gacagtatgg acggcttttt
    16441 cactcgctac ggcaaacaca ccatcctggt ctgtcggtta ttgccttttg tccctttcga
    16501 tccaatcagc tatgctgccg gtctgacttc aatacgtttt cgctcgtttt ttatcgccac
    16561 cgggcttggt caattaccgg cgactattgt ttattcctgg gcaggcagca tgttaacagg
    16621 cggtactttc tggtttgtca ccgaactgtt tattctgttt gccctgaccg tggtgatttt
    16681 tatggcgaag aaaatatggc ttgagcgcca gaagaggaat gactgatggg tttaccgccg
    16741 cttagcaaaa ttcctttcat tttacgtccg caggcgtggc tgcatcgtcg ccattacggc
    16801 gaggtgctaa gcccgattcg ctggtggggg cggatcccgt ttatctttta tttggtgtca
    16861 atgtttgttg ggtggctgga gcgcaaacgc tcaccgctcg atccggtagt gcgatcgctt
    16921 gtcagcgcgc gcattgcgca aatgtgcctg tgtgagtttt gcgtggatat caccagtatg
    16981 aaagtcgccg agcgtaccgg cagcaccgat aaactgctgg cagtggctga ctggcggcaa
    17041 agcccgctct ttagcgatga agaacggctg gcgctggagt acgccgaagc cgcaagcgtc
    17101 acaccgccaa cagtcgatga tgccctgcgt acccgactgg ctgcgcattt tgacgctcag
    17161 gcgctcaccg aactgacggc attgatcggc ctgcaaaacc tgtcggctcg ttttaattct
    17221 gccatggaca ttcccgctca ggggctgtgc cgtattccgg aaaaacgttc ttaaggaggg
    17281 aagatgcgcc attgtgggtg gttgctggga ttgttatcgc tgttttcttt ggcaacacat
    17341 gccagtgact ggcaggaaat taaaaatgag gccaaagggc aaaccgtctg gtttaacgcc
    17401 tggggcggcg ataccgcaat taaccgctat ctcgactggg ttagcggcga gatgaaaacc
    17461 cattacgcta taaacctgaa gattgttcgt ctcgcggatg ccgcagacgc ggtgaagcgc
    17521 attcagaccg aagctgctgc cggtcgtaaa acgggcggct cggtggatct gctctgggtg
    17581 aacggcgaaa acttccgcac ct
//
LOCUS       DAZOYF010000077        17403 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZOYF010000077 DAZOYF010000000
VERSION     DAZOYF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.077"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHUOF_002411"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHUOF_002411"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR0611556.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHUOF_002412"
     CDS             complement(1672..2667)
                     /locus_tag="ACHUOF_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0611557.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHUOF_002413"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHUOF_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHUOF_002414"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHUOF_002414"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR0611558.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHUOF_002415"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHUOF_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR0611559.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHUOF_002416"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHUOF_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR0611560.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="ACHUOF_002417"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="ACHUOF_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR0611561.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="ACHUOF_002418"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="ACHUOF_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR0611562.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHUOF_002419"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHUOF_002419"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR0611563.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHUOF_002420"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHUOF_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR0611564.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHUOF_002421"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHUOF_002421"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR0611565.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHUOF_002422"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHUOF_002422"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR0611566.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         5029 a         3990 c         3742 g         4642 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
    17401 gtg
//
LOCUS       DAZOYF010000078        17374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZOYF010000078 DAZOYF010000000
VERSION     DAZOYF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.078"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHUOF_002423"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHUOF_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR0611567.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="ACHUOF_002424"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="ACHUOF_002424"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR0611568.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /locus_tag="ACHUOF_002425"
     CDS             2826..3980
                     /locus_tag="ACHUOF_002425"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR0611569.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHUOF_002426"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHUOF_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR0611570.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="ACHUOF_002427"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="ACHUOF_002427"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR0611571.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="ACHUOF_002428"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="ACHUOF_002428"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR0611572.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHUOF_002429"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHUOF_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR0611573.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="ACHUOF_002430"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="ACHUOF_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR0611574.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="ACHUOF_002431"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="ACHUOF_002431"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR0611575.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="ACHUOF_002432"
     CDS             11296..11511
                     /locus_tag="ACHUOF_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR0611576.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHUOF_002433"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHUOF_002433"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR0611577.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="ACHUOF_002434"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="ACHUOF_002434"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR0611578.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="ACHUOF_002435"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="ACHUOF_002435"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR0611579.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="ACHUOF_002436"
     CDS             complement(16247..16786)
                     /locus_tag="ACHUOF_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR0611580.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="ACHUOF_002437"
     CDS             complement(16802..17320)
                     /locus_tag="ACHUOF_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0611581.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT         4028 a         4320 c         4825 g         4201 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa agag
//
LOCUS       DAZOYF010000079        17266 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZOYF010000079 DAZOYF010000000
VERSION     DAZOYF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.079"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            326..448
                     /locus_tag="ACHUOF_002438"
     CDS             326..448
                     /locus_tag="ACHUOF_002438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611582.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     gene            445..1011
                     /gene="mntP"
                     /locus_tag="ACHUOF_002439"
     CDS             445..1011
                     /gene="mntP"
                     /locus_tag="ACHUOF_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR0611583.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="ACHUOF_002440"
     CDS             complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="ACHUOF_002440"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR0611584.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="ACHUOF_002441"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="ACHUOF_002441"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0611585.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2205..2348)
                     /locus_tag="ACHUOF_002442"
     CDS             complement(2205..2348)
                     /locus_tag="ACHUOF_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR0611586.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(3020..3307)
                     /locus_tag="ACHUOF_002443"
     CDS             complement(3020..3307)
                     /locus_tag="ACHUOF_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR0611587.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3382..3525)
                     /gene="mgrB"
                     /locus_tag="ACHUOF_002444"
     CDS             complement(3382..3525)
                     /gene="mgrB"
                     /locus_tag="ACHUOF_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR0611588.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3684..3923
                     /locus_tag="ACHUOF_002445"
     CDS             3684..3923
                     /locus_tag="ACHUOF_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR0611589.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(4066..4857)
                     /gene="kdgR"
                     /locus_tag="ACHUOF_002446"
     CDS             complement(4066..4857)
                     /gene="kdgR"
                     /locus_tag="ACHUOF_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR0611590.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            5034..6407
                     /locus_tag="ACHUOF_002447"
     CDS             5034..6407
                     /locus_tag="ACHUOF_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0611591.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6453..7334)
                     /gene="htpX"
                     /locus_tag="ACHUOF_002448"
     CDS             complement(6453..7334)
                     /gene="htpX"
                     /locus_tag="ACHUOF_002448"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR0611592.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMMMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7526..9574)
                     /gene="prc"
                     /locus_tag="ACHUOF_002449"
     CDS             complement(7526..9574)
                     /gene="prc"
                     /locus_tag="ACHUOF_002449"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR0611593.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9594..10292)
                     /gene="proQ"
                     /locus_tag="ACHUOF_002450"
     CDS             complement(9594..10292)
                     /gene="proQ"
                     /locus_tag="ACHUOF_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR0611594.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10389..10886)
                     /gene="msrC"
                     /locus_tag="ACHUOF_002451"
     CDS             complement(10389..10886)
                     /gene="msrC"
                     /locus_tag="ACHUOF_002451"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR0611595.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            11016..12299
                     /gene="yebS"
                     /locus_tag="ACHUOF_002452"
     CDS             11016..12299
                     /gene="yebS"
                     /locus_tag="ACHUOF_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR0611596.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12268..14888
                     /gene="yebT"
                     /locus_tag="ACHUOF_002453"
                     /pseudo
     CDS             12268..14888
                     /gene="yebT"
                     /locus_tag="ACHUOF_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14968..16407
                     /gene="rsmF"
                     /locus_tag="ACHUOF_002454"
                     /pseudo
     CDS             14968..16407
                     /gene="rsmF"
                     /locus_tag="ACHUOF_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16525..16761
                     /locus_tag="ACHUOF_002455"
     CDS             16525..16761
                     /locus_tag="ACHUOF_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFR0611597.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16866..17057
                     /locus_tag="ACHUOF_002456"
     CDS             16866..17057
                     /locus_tag="ACHUOF_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR0611598.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(17082..>17266)
                     /locus_tag="ACHUOF_002457"
     CDS             complement(17082..>17266)
                     /locus_tag="ACHUOF_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR0611599.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
BASE COUNT         4188 a         4355 c         4160 g         4563 t
ORIGIN      
        1 gtatcaactc aataacttat gaatttagcc aaagctatgt ttagtgtatt tttaataatc
       61 agacatagct taggctatat tatctcttcc cttatttgtt atttatttta acgtttcatt
      121 gatatataaa cataaatgaa aatcgttatc aataaagcaa tgaaataata tattccaaca
      181 gttgttttat attctcaaaa tatgttaagg ttgcgccctc atttggggag tagccgattt
      241 ccagattccg gaaatgtacg tgtcaacata ctcgttgcaa aacgtggcac gtacggactg
      301 aagactttca gtcaggcgag accatatgca catcaatcgc tatgcctgca tgaacgcgaa
      361 aacccatcgc ggcttgttgt tcatgtatgc atgtttgctg ggggcgatga tgtgttttat
      421 ggataccccg gtcaggacgt tgtcatgaat atcactgcta ctgttcttct tgcgtttggt
      481 atgtcgatgg atgcatttgc tgcatcaatc ggtaaaggtg cccccctcca taaaccgaaa
      541 ttttctgaag cattgcgaac cggccttatt tttggtgccg tcgaaaccct gacgccgctg
      601 atcggctggg gaatgggcat gttagccagc cggtttgtcc ttgaatggaa tcactggatt
      661 gcgtttgtgc tgctgatatt cctcggcggg cgaatgatta ttgagggttt tcgtggcgca
      721 gatgatgaag atgaagagcc gcgccgtcgg cacggtttct ggctactggt aaccaccgcg
      781 attgccacca gcctggatgc catggctgtg ggtgttggtc ttgctttcct tcaggtcaac
      841 attatcgcga ccgcattggc cattggttgt gcaaccttga ttatgtcgac attagggatg
      901 atggttggtc gctttatcgg ctcaattatt gggaaaaaag cggaaattct cggcgggctg
      961 gtgctgatcg gcatcggcgt ccagatcctc tggacgcact tccacggtta ataagaacgc
     1021 tgccagaggt gaatattaaa gtccgtctgg cagtcgaaca cttcttttgc tgccagtgtt
     1081 tgccagactt ctggcttcgc acgccaggca aacggcgtca tctgcaataa tgcgacggct
     1141 tcatcaccgc gaagacgcat cggataacac aactccgcac tctgctgtaa tgtaaaacct
     1201 tccagttgtt ctgcatgagg tgcatgaaga tgtacttcat tgtaaatcag ccccttcagc
     1261 tccatcaaat gtcgcggtcc cggcgtggca gtaatgaccc agccgccggg cttcactact
     1321 cgtgctaatt cttctgcttt acacggcgcg taaatacgta ttatggcgtc catactggta
     1381 tcggaaaacg gcaaacggtg gctggaagcg acacaaagag tgacctgcgg atagcgtttc
     1441 gccgccgctt ttatcgctac cttcgagaca tccaggccaa acgtggtgat ttcgggcaac
     1501 gcatcggcaa atgcgtgtgt gtaataccct tcaccacagc caatatccag caccgccgtg
     1561 gccttttcat caagccgttc ccgcagttgg gcgacaattg catcacgcag cggctgataa
     1621 tgtccggcat ctaagaatgc gcggcgtgct tgcatcattt ccgcgctgtc gcccggatca
     1681 cgagaccgtt tatgctgaac gggcagcaga ttgacatacc cttctttcgc catatcaaac
     1741 tgatgtcgtt ggggacagat atagctgttt ttttcacgcg aaagaggctg atggcaaagt
     1801 ggacaggaaa acgacatgac aactccggca ggatactaaa ggccgcaagt gtaacgcgaa
     1861 tcacgcggcg ggcaaatatc cgaacgccag gagcgaagat aaagcatagt aaaaagcctc
     1921 gcattcgacg aggctttata gcgagattga agcgtattca cacttcagat cagtggattc
     1981 gatcagatag ctgttacgtt aacagctgcc ggacctttct ggccgtcctg aatttcgaac
     2041 tcaacgttct gaccttcagc cagagttttg aagccattac cctggatagc ggagaagtgt
     2101 acgaacacat ctttgctgcc atcagccgga gtaatgaagc caaaaccttt agactcgttg
     2161 aaccacttaa cctgaccttt aatctttgcc atttgaaaaa ttccttagat tgttttcttc
     2221 gcccgcaggc ataacataga taaaactgac acattactgc atgaggcacc aatataaggc
     2281 tcggcagaga agcggtattc aacgtcaacg tgtttactca ggacttcttt actgaaaatg
     2341 ccacacataa acagaactgt acctcgttta acccgaaatc ttttatcaca tacaacgtta
     2401 attatggcaa gccattttta aacatatctc gatcagacgc acaaatctcg atacgtcttc
     2461 cacttttttt gcacacttat gcaacggaat acgcgccgat atatcattgt gcttaacctt
     2521 gccagttcag gcagatactt aaactggcgt agtttctaac atagttcaat aagttcagtt
     2581 gttccaggcc agagggagaa gttattacat agtgcgtgca atatcacatt ttctgctatg
     2641 caatgaataa aaagttatat cactttttct cataaaacag tcagttaacg gctatcaatt
     2701 accctaaaga gaagtcaatc cccaaaggga ttgtaaattt aaaataagaa aaattgatga
     2761 gtgagcaaaa aaaaatcaag agagaaacgt ttcgctaata aataatacca cgcttaatca
     2821 caaatctgat gtaaacatcc taataaatta atgaggaaaa tatctttttc tgatatcccg
     2881 ttacgcaaat aaataatatg ataattattt gaagtcagac cagagtttta atttgaagca
     2941 gttgcaccat gacagtgcgt ataatcagga tgaattgagt aacccttcaa cgacggggtt
     3001 actcaagatt gaggaaggat tatcgttcgg cgaccaggcg aataatatct tcgtcatcaa
     3061 ccgatttttg actctcaatg gtctttttat ctgctttctc tggttcgttt gcttcgcaca
     3121 aacgacgtaa cagtgcattt tgacgttttt gctgatccag caacgcctca agcagttcaa
     3181 tctgttcgtt agtccgtgaa ctggcacgat tgataaaaaa ccacaagatg agcccgataa
     3241 gaagaaccac caccgataca accaaagacg caatgttcat cacgcctgaa tttacaactt
     3301 cgttcatttc accacctctg agtagaggcg ctattctacc actgctggag aggaagaaaa
     3361 tctagtgctg aaaaaatgat atcaccacgg gataaactgg ttaatggcac aaattccgct
     3421 gaaaaattgt acatcctgat cgcacatcat gttgaatacc tgcgcccaaa gcagcaagca
     3481 agccaacacc acgacaacca gaacgaccca tcgaaacttt ttcactccaa tctccgcttc
     3541 acctacctta tgtcattaaa ctagcatgcg ttagttaaac agcgcctaac tatgtcgata
     3601 ttcgtgcttt ttcggaatga gtagcttgct tcagcgcata ttgccgctac gattaagcga
     3661 acataaaaag agaagagatt gtaatgcgat tcatcattcg cacagttatg ctgatagcac
     3721 tggtatggat aggattatta ctcagcggat atggcgtttt gattggaagc aaagagaatg
     3781 ccgcaggatt aggtttgcaa tgtacctatc tgactgccag aggcaccagc acggtacaat
     3841 acttgcacac taaaagtggt ttcttggaga taacagattg cccactgtta cgtaaaagca
     3901 atatcgtggt cgataatggt taagggtaat aaataaccag attctcactc atgagtaaaa
     3961 tattcttata gcaagcgcgt ttaatataac gcaccttcaa ctttattttt ctcatgctaa
     4021 ataaagaacc tgtctgtttg atattccgac aggttcttac tctcatcaga acggatagtc
     4081 gtgataaccc atttgggcag aaattttgcg cgctgcggta tgcaacattg cgacatattc
     4141 ctgtaaacgc tcttcagaga aacgcaacgt cgggaaggag atgctcaaac cggcaatgac
     4201 cacgccaaag cgatcaaata ccggtaccgc aatgcattgc agcccttctt cctgctcttc
     4261 gttatcttct ccatacccct gctcgcgcac ctggtccaga acgggtaata acgcttctgt
     4321 actggtgatg gtccgctcgg tactgcgttt atactccacg ccctcaagaa tttgcttcac
     4381 ttcatcgcga tcgcgccatg ccagcagtac cttaccaatc gcggtgctgt acagcggatt
     4441 acgacgccca atccgtgaat acatgcgcaa attgtacata gagtcaattt tgtgaatgta
     4501 aacaatactg tcttcgtcca gtgcgccgag gtggatagtt tctttggtca ggcgggagat
     4561 ctcacgcatc tggatatctg cgctacgaat taaatcgacg ttttgtaacg cgcgagcgcc
     4621 cagttcaaac aatttcaggg tcagggaata tttctccgac tccccttcct gcgccacata
     4681 acctaaggtt ttcatggtct gtaaaaagcg ataaacggtg ctttttgaca tcatgacgcg
     4741 ctgcgacagc tcggttatcc ctatttcgcg ctcttcaccc agcgcctgca aaatgccaaa
     4801 aacttttagc acggaagata cagaatcagg ctgtttatcc agatctgcgt tagccattta
     4861 tcacctcatt gcgagtgttt tataaaaatc agaactgttt tttattataa tttcgcacca
     4921 gggtggtcgc aatccatctt ttgccggtta gttacaattc tgcgacatcc accgtgaata
     4981 tcagtgctag aatcataccc ctgttgatta ttcaccaaag atataaaatt cctatgccaa
     5041 aagttcaggc cgacggcctg ccattgcccc agcgatacgg tgcgatatta accattgtga
     5101 ttggtatttc gatggctgtc cttgacggcg caatcgccaa cgtcgccctg ccaacaattg
     5161 ccacggacct tcatgccaca ccagccagtt ccatctgggt agtgaacgcc tatcaaatcg
     5221 ccattgtcat ctccctgctc tcgttttcgt ttctgggcga tatgtttggc tatcgacgta
     5281 tttataaatg cggtctggtc gtttttctgt tgtcttcact gttctgcgcc ctttctgatt
     5341 cgctgcaaat gctcaccctt gcgcgtgtca tacaaggttt cggcggtgca gcgttgatga
     5401 gcgttaatac cgcacttatc cgcctgatct atccacaacg ttttctgggg agagggatgg
     5461 gcataaactc gtttattgtt gccgtctctt ctgctgccgg gccgacaatt gctgcagcaa
     5521 tcctctccat cgcatcctgg aaatggttat ttttaatcaa cgtaccgtta ggtattatcg
     5581 ccctgcttct ggcgatgcgt tttctgccac ccaatggttc tcgcgccagt aaaccccgtt
     5641 tcgacctgcc cagcgccgtg atgaacgcgt taaccttcgg cctgcttatc actgcgttga
     5701 gtggtttcgc tcaggggcaa tcgctgacgt taattgctgc ggaactggtg gtaatggttg
     5761 ttgttggtat tttctttatt cgccgccagc tttctcttcc cgtaccgctg ctaccggtgg
     5821 atttactgcg tatcccgctg ttttcacttt ctatttgcac atctgtttgc tctttctgcg
     5881 cacaaatgct ggcaatggtt tccctgccct tttacctgca aaccgtgctc gggcgtagtg
     5941 aagtcgaaac aggtttactt ctgacaccgt ggccgttagc aacgatggtg atggctcctc
     6001 tggcaggcta tttgattgaa cgcgtacatg caggattgct gggtgcttta gggttgttca
     6061 tcatggctac ggggcttttt tccctggttc tgctgcccgc gtcacctgcg gatatcaata
     6121 ttatctggcc gatgatctta tgtggcgctg gatttggctt attccagtca cccaataacc
     6181 acaccattat tacctccgct ccgcgcgaac gtagcggtgg ggccagtggc atgttaggaa
     6241 cggctcgtct actgggtcag agtagtggcg cggcgctggt ggcgctgatg ctaaatcagt
     6301 ttggtgataa tggtacgcac gtctcgctga tggctgcggc tattctggca gtgattgctg
     6361 cctgtgtcag tggtttacgt atcactcagc cacgagccag ggcataaaaa aagcgcgtcg
     6421 atcaggacgc gctttttagt atttacttca tattacttca ggtattcacc cgtacgcaga
     6481 gcttcaatac gtttatccag cggcgggtga gtcatgaaca actcactgag cgatttcgac
     6541 ttaccgttaa tgcagaaagc catcatgctg gttgcttctt gcggttcata gctggttttc
     6601 aggcgctgca gcgcggcaat cattttctcg cgaccaacca gttttgccga accggcatct
     6661 gcatggaatt cacgatgacg cgagaaccac atggtgataa tgctcgccag aataccaaac
     6721 accagttcca gaaccgttgc aaccgcaaag tagatcagcg ggttgccgtt gctctcttca
     6781 ccttcatcac gatttccgcc cataaaaccc gcggcaagct gcgccagaat acgggaaata
     6841 aagataacga aggtgttcac cacgccctga atcagcgtca tggtgaccat atcaccattg
     6901 gcgatgtggc tgatttcgtg agcaattacc gcctcggctt catccgggct catgttctgc
     6961 agcaaaccgg tgctgacagc aaccagagag gcatcacggc gcgcaccggt tgcaaaagcg
     7021 ttgatgtccg gcgcatggta gatagccact tgcggcatag cgatccccgc ctgacgagcc
     7081 tgggttgcta cagtattgac cagccaacgt tccctttcgt tacgcggttg ctcgatcact
     7141 tccccgccaa cagatcgtaa tgccatccat ttggacatca gaagcgaaac gaaggaacca
     7201 ccaaaaccga acagcaaggc catcatcatc agcccctgaa cgctgctcga ctgtatccct
     7261 gtcaggctca gtaccagccc gaaaacgacc attacggcca ggttcgttag caggaagagc
     7321 gcgattcgca tcataatttt ctttttacct cagtttaaca aaacgcaata tgcgataccc
     7381 acatcgtatg ggttacgcga ctattttcaa gtctggatag tgcgtaagtc accagaaaga
     7441 cacaacttta cattttgtag catctgattt acggcatctt gccgctgtta aaaaatcagg
     7501 cacaatttct tgtgcctgat tgatattact tgacgggagc gggttgttcc gcgggtctgg
     7561 ctttttcaag cttcgccaga tcgagtgcga tattcaccgt ctcatccaga taaggatccg
     7621 gctcctggta atctttcgat agatcatcca gcttcttcaa ctccggttta ccttctcgtt
     7681 taaagcgttc gttcaaacgc gccagacgcg tcgcatcatc ttcattattc tctttctcac
     7741 gcacagcgta gttcagagaa acgatattgc gcttgtcctt catagcgttg aagcgcgcga
     7801 tatccttcat gatgttctgg aactcaggat ctttcgcgat acgcgcatta tgttccttca
     7861 gcagctccgg ttcaaaggca gttaaatctc ctgatttcac ataagtcgcg gcatcgatgc
     7921 tatcccacgg cagcgcgtta tcttcgaatt tctcacccgt ttccgtttct tcattacccg
     7981 tcggcatgat gatgtctggt gttacgcctt tacgttgcgt actgccgccg ttaacgcgat
     8041 agaatttctg gatcgtgtac tgcacagaac ccagcgctgg ccattcagga cgtaacatct
     8101 gatcgtaaat acggttcaat gaacggtatt gctgaacggt gcctttacca aacgtcggtt
     8161 cacccacaac cagcgcacga ccgtaatcct gcattgccgc ggcaaagatt tctgaagccg
     8221 aagcactgaa acggtcaacc agtaccacca gcgggccttt atagaacacc tgcccgtcgg
     8281 tatcgctgtc ttcacgaacc ttgccgttgt tatcgcggac ctgaacaatg ggacccgcag
     8341 gaataaacag acctgagagc gataccgctt cggtcaacgc cccaccgcca ttgctacgca
     8401 ggtcgatgat cacactgctg acattctgtt tttccagttt ctgcagttga actttgacat
     8461 cgtctgtcaa acccacatag aagcccggaa tatccagcac gccgactttc tctttaccga
     8521 cggtcttcac cgacatttta accgcgcggt cttcgagacg aatacgttca cgggtcaacg
     8581 ttacagtacg ggtcttggtc cctttaccag caggtaaaat ttccagacga actttactgc
     8641 ccttcggccc tttaattaag gcaaccacat catcaagacg ccagccaatc acgtcaacca
     8701 tcggcttgcc tgtttgacca acaccgacaa ttttgtcacc aacgctgata gctttactct
     8761 tcgctgccgg accacctgcc accatcgaat tgataacggt gtagtcatca tccatttgca
     8821 gcactgcgcc aataccttcc agtgacaaac tcatttcagt gttgaactgt tcggtattac
     8881 gcggggaaag atagttggta tgcggatcga tttcacgcgc aaacgccgtc attgccagcg
     8941 agaaaacatc ttcgctgttg gtttgcgcca gacgacgaat ggcaaatttg tagcggcgtg
     9001 tcagggtttc acgaatttct ttatccgttt ttcctgtcag cttcaggctt aactcgtcga
     9061 atttgacttt actgtcccac tgcgcgttca actcagcctc gtttttcggc cagggcgctt
     9121 tgctgcggtc aaggttataa gtgtcgttgc cggtgaaatc catcggcttt tccagtaccg
     9181 acaaagcgta ctggtaacgt tcaaaacggc gcttttgcgc cagattgtag agatcgtaga
     9241 aaacgtcgag tttgcctgaa cgcagttcat cgcctaactc ggttttcttt ttcgcgaact
     9301 gttcaacatc gcttgccagc agcacattgt ggctgtaatc gagcagattc aggtagcggt
     9361 caaagatttt ggccgaaaat gcctgatcga ggtcgaactg gcgataatga gaacgggtga
     9421 agcgcgacgt tacgcgctca cttaccgtcg catgctgcgt ctcttccttt aataccggaa
     9481 tttgatcagc acgcgtgata tcttctacag cgaaggtctg gcctgctatt gcaagcaggc
     9541 cagctaacgc ggtaagccta aaaaacatgt tcatgcctgg cccggcctcc gtttcagaac
     9601 accaggtgtt ctgcgcgcac aatcaaagac atacccgaat tcagctggac gcggacgccg
     9661 tctttggtga tttctaatac ggtggcatcc atcgcgtttt gacccgcttt caccttcagg
     9721 gcttgtccga cagtcagagc tgaaatgtca gaaaccgggg tgtgctgttc ttcgcgaggt
     9781 gcttttactg tttttggcgc tttctctacc ggcttttgcg cacgaggttt acgttcagcg
     9841 ccttctttgc ggcgtggcgt ggtcggacgt ggcttgcgtt cgcggcgcgg tgcgtcttct
     9901 ttctcaccag cagctgcggc agcttcgcgt tttttcgctt gctgttcagc acgctgtgcc
     9961 tgaacacgcg ctttcgcttc ttcaagctgc ttgcgagcat gctctacatg ttgctcgtcc
    10021 agctcaccgc atgggttgcc gtcaagatcg acacgcgttg cgccgggttt aacaccgtaa
    10081 agataacgcc agctcgaagt gtagagacgt aaagcggatc gtaattgcgt tttgctcagg
    10141 ttcatttccc cggcaacacg atcgaccaaa tcctgaaaaa taccaatttt cagcggacgc
    10201 gcttcacctt ccgcactgaa acagtgggga aaacgttcgg ccagaaacgc gattacttct
    10261 ttactgctat tcaacttagg ttgattttcc atgaaatttc ctgattacaa cggacgtagc
    10321 caacaagcgc aggcatgaac aggcgtcatt ataatgacgc taccagtaaa tgctacgtta
    10381 tccgttgatt atcctgcgac gctcgcaaag aattttttgt aatccgtcgt tgcaagcact
    10441 ttttcaagct gtgccacaag ctgacgtaag ccttgctcgt cctcgtctgt aaagcgagcg
    10501 aagacagtac tatcgatgtc gagaacacca ataatctgat ttttcaccac cagcggcaga
    10561 acaatttcag aattactcgc cgcatcacag gcaatatgcc cgtcaaacgc gtgaacatcc
    10621 tcgatacgct gcacttgatt gcgggcaacc gctgtgccgc acaccccgcg cccgacgggt
    10681 atccggacac aggcaatttt gccctgaaat ggtccgagta ccagtgtatc gtcctcaagc
    10741 aaataaaaac ctgcccagtt aacgtcagtg agacgctcgt ataacaacgc actggtgttc
    10801 gcaagcgttg ccagaaaact ggtttctccc gccatcagcg cgttaaagtc gcgatttaaa
    10861 tccgcgtaaa attctgtttt gttcattact taatcacttg gttgtcttac ctggaactgc
    10921 cagcctatta aaataagcat taaatgcgtt aatgctcaag atcattccca tcatgggtta
    10981 agattaatgt taattcttat tacatttggc acgtcatggc tcttaacaca ccacaaatta
    11041 cgccgacaaa aaagataaca gtgagggcaa tcggcgagga actgccgcgt ggtgattacc
    11101 aacgttgccc gcaatgtgac atgctgttta gcctgcccga gataaattct catcaaagtg
    11161 cctattgtcc gcgctgtcag gcaaaaattc gcgacgggcg cgactggtcg ctaacgcgcc
    11221 tggcggcaat ggccttcacc atgctgttgc ttatgccgtt tgcctggggc gaaccgctgt
    11281 tgcatatctg gctgttaggc attcgtattg acgccaacgt catgcaaggc atctggcaaa
    11341 tgaccaaaca gggcgatgcg ataacgggat caatggtctt tttctgcgtt attggtgccc
    11401 ccctcattct ggtgtcctcc atagcatatt tatggtttgg taaccgactg ggaatgaatt
    11461 tacgtccggt actgctgatg cttgagcgac ttaaagagtg ggtaatgctg gatatctacc
    11521 tggtcggcat tggcgttgct tctataaagg tacaggatta tgcccatatc caggcgggtg
    11581 tcggcttgtt ctcttttgtg gcgttggtga ttttaacgac ggtgacgttg tcacatctta
    11641 atgttgagga gctgtgggag cgatattatc cgcagcgccc cgctacgcgt agggacgaga
    11701 aacttcgtgt ctgtcttggg tgccatttta ccggctatcc tgatcagcgt ggtctctgcc
    11761 cgcgttgcca tatcccgcta cgcctgcgtc gtcgtcatag tttgcaaaaa tgctgggcgg
    11821 cgctgttagc gtcaatcgtt ttgttgttac cagccaacct attgcctatt tctatcattt
    11881 atctgaatgg tggccggcag gaagatacga ttctttccgg aattatgtcg ctggcaagta
    11941 gcaacattgc ggttgcggga atcgtgttta tcgccagtat tctggtaccg tttactaaag
    12001 tgatcgtcat gttcacttta ctgttgagca ttcattttaa atgccagcaa ggtttacgca
    12061 cacgcattct gttactgcgg atggtgacct ggattggacg ctggtcgata ctcgacctgt
    12121 ttgtcatatc tttaaccatg tcgctgatta atcgcgatca gatcctcgct tttactatgg
    12181 gaccggctgc gttttatttt ggcgctgccg taattttgac tattcttgct gtggaatggc
    12241 tggacagccg cttactttgg gatgcacatg agtcaggaaa cgcccgcttc gacgactgaa
    12301 gcgcagatta aaaataaacg ccgtatctca cctttctggc tgctgccttt catcgcgcta
    12361 atgattgccg gttggctgat ttgggacagt tatcaggacc ggggtaatac cgtcaccatc
    12421 gactttatgt cggcggatgg tattgttccg ggccgtacgc ctgttcgtta tcagggcgtt
    12481 gaagtcggaa cagtgcagga tatcagcctc agcgacgatc ttcgtaagat tgaagtcaag
    12541 gtcagcatca agtccgatat gaaagatgcg ctgcgcgaag agactcagtt ctggctgatg
    12601 acgccaaaag catcgttggc aggtgtctcc gggctggacg ccctcgtcgg tgggaactat
    12661 atcggcatga tgccgggtaa aggtaaagag caggatcact ttgtcgcgct cgatacccaa
    12721 ccgaaatatc ggctggacaa tggcgatctg atgatccacc tgcaagcccc cgctctcggt
    12781 tcgctgaaca gcggttcatt ggtctatttc cgcaagatcc cggtgggaaa agtctacgac
    12841 tatgccatca atcccaacaa gcaaggcgtg gtgattgatg tcctgatcga gcggcgtttt
    12901 accgatctgg tgaaaaaagg tagccgtttc tgaaacgttt ccggcgttga tgccaacgtc
    12961 agtatcagtg gcgcgaaggt gaaactggaa agtctggcgg cactggttaa cggtgcgatt
    13021 gccttcgatt cacctgaaga gtcgaaacct gccgaggcgg aagatacctt tggtctgtat
    13081 gaagatctgg cccacagcca gcgtggagta ataataaaac tggaactgcc gagtggagca
    13141 ggattaaccg ccgactcgac gccgttaatg tatcaggggc tggaagtcgg acagctgact
    13201 aaactggatt taaatcctgg tggtaaagtc actggggaaa tgaccgttga tccctgcttc
    13261 gtgaaaatac ccgcatcgaa ttacgcaacc cgaaattatc ccttagcgat gctaatctca
    13321 gcgccctgct gaccggaaaa acctttgagt tggtgcccgg cgatggcgag ccacgcaaag
    13381 agttcgttgt tgtgccgggc gaaaaagccc tgctgcaaga gccggacgtt ctgacactga
    13441 ccctgacagc accggaaagt tacggtattg atgcgggtca gccgctcatt cttcacggcg
    13501 tgcaggtagg ccaggttatc gatcgtaaac tcaccagcaa aggcgtcacc tttaccgtcg
    13561 ccatcgagcc tcagcatcga gaactggtaa aaggcgatag caaatttgtc gtcaacagcc
    13621 gtgtcgacgt gaaggtgggg ctggatggcg ttgagtttct cggtgccagc gcctcagaat
    13681 ggattaacgg cgggatacgt attctgccgg gcgataaagg cgagatgaaa gccagctatc
    13741 cactgtatgc caatctggaa aaagcgctgg agaacagcct tagcgattta cccaccacaa
    13801 ccatgagttt gagtgcagag acgctgccgg atgtgcaggc aggatcggta gtgctgtacc
    13861 gtaaatttga agttggtgaa gttattaccg tgcgtccgcg agctaacgcg ttcgatatcg
    13921 atctgcacat taaaccggaa tatcgcaacc ttttgaccag caacagcgtg ttctgggcag
    13981 aaggcggggc gaaagttcag ctgaatggta gtggcctgac cgtacaggca tccccgctct
    14041 ccagagcatt aaagggggcc attagcttcg ataacctcag tggtgccagc gccagtcagc
    14101 gtaaaggcga caagcgaatt ctgtatgctt ccgaaacagc ggcccgtgcg gttggcaggc
    14161 agattacgct tcacgctttc gatgccggaa aactggcggt cgggatgcca attcgctatc
    14221 tcggtattga tatcgggcaa atccagacgc tggatctgat taccgcacgc aatgaagtac
    14281 aggcaaaggc ggtgctctat ccggaatatg tccagacctt tgctcgcggt ggtacgcgct
    14341 tctcagtggt cacaccgcaa atctcggcag ctggcgttga gcatctagat actatcctcc
    14401 agccgtatat caacgtcgaa ccaggccgtg gcaatcctcg tcgcgacttt gaattagaag
    14461 aggccaccat tactgattcg cgttacctag atggtttaag cattattgtt gaagcgccgg
    14521 aagccggttc gttaggcatc ggtacgcctg tgctgttccg tggtctggaa gtaggtacgg
    14581 ttaccggaat gacgctgggg acattgtcag atcgcgtgat gattgcgatg cgcatcagta
    14641 aacgctatca acacctggtg cgtaacaatt ccgtcttctg gttggcatcg ggttacagtc
    14701 tggactttgg tctgacgggc ggcgtagtga aaaccggcac ctttaaccaa tttatccgtg
    14761 gcggcatcgc cttcgccacg cctccgggta cgccacttgc accgaaagcc caagaaggca
    14821 aacacttcct gttgcaggaa agtgaaccga aagagtggcg tgaatgggga actgcgcttc
    14881 ccaaataatg cccactgctc cggcgtgcct gcgccggagc gcttatgcta aactgcgcgc
    14941 ctgttttttt gccagtggta catgctcgtg gcccaacaca ccgtttattt cccggacgcg
    15001 tttctgacac aaatgcgcga agctatgcct tcgacgctct catttgatga ttttcttgcc
    15061 gcctgtcagc gcccgttgcg ccgcagcatt cgcgttaata cgctgaaaat ctccgttgct
    15121 gatttcctgc aattaaccgc tccttatggc tggacgctta cgccaattcc gtggtgtgaa
    15181 gaaggtttct ggattgaacg cgacaatgaa gatgcattgc cattgggtag taccaccgag
    15241 catttaagtg gcctgtttta tattcaggaa gccagttcaa tgttgcccgt cgccgccttg
    15301 tttgctgacg gtaatgcacc acagcgggtg atggatgtcg ctgctgcgcc aggctcaaaa
    15361 acgacgcaaa ttgccgcgcg gatgaataac gaaggggcaa tccttgccaa tgagttttcc
    15421 gccagttggg taaaagtgtt acatgccaat atcagccgct gtggcatcag taatgttgcg
    15481 ctcacacatt ttggtggccg cgtgtttggt gcggcagtgc cagaaatgtt tgatgccatt
    15541 ttgctggacg ctccctgctc cggcgaaggc gtggtgcgta aagatcccga tgcgctaaaa
    15601 aactggtcac ccgaaagcaa tcaggaaatc gcagctaccc aacgggaact gattgacagc
    15661 gcctttcatg cattacgtcc tggcggtacg ctggtttact cgacctatac cttaaaccgg
    15721 gaagaaaacg aagccgtttg ccggtggctg aaagagactt accccgacga agtagagttt
    15781 ttaccacttg gcgatctctt ccctggtgca aacaaggcgc tgaccgaaga aggttttttg
    15841 catgttttcc cacaaattta cgactgcgaa ggcttcttcg ttgctcgtct gcgtaaaact
    15901 caggcgatcc ccgccttacc cgccccaaaa tacaaagtgg gcaatttccc gttcagcccg
    15961 gtgaaagatc gcgaagccgg acaaattcgt caggcggctg caagtgttgg cttaaactgg
    16021 gatggaaacc tgcgactctg gcaacgcgac aaagaactgt ggttgttccc ggtaggcatt
    16081 gaagccctga tcggtaaagt ccgattttct cgcttaggga ttaaacttgc cgaaacgcat
    16141 aacaaaggtt atcgctggca gcatgaagcg gttattgccc atgcctcccc cgacaatgta
    16201 aacgcttttg aactgacacc gcaggaagcg taggagtggt atcgcgggcg cgatgtttac
    16261 ccgcaagccg cgccagtggc ggatgacgtg ttggttactt tccagcatca gccgattggt
    16321 ttagccaaac ggattggttc gcgactgaaa aacagctacc cgcgtgaact ggtgcgggac
    16381 gggaaacttt ttaccagtaa cgcatgacag cgcacaaaaa aagcgcactt tttgactggc
    16441 acattcgccg gcctcaacta ggctgaaaaa ttgtgcgatc ggactggtcg tactacaatc
    16501 agcagctaaa tggagagcac gaagatgaaa accagtgtgc gcataggcgc ttttgaaatc
    16561 gacgacggcg aattacacgg tgaatcgccg ggtgatcgaa cgttaaccat tccttgtaaa
    16621 tctgaccccg atttatgtat gcaactggat gcctgggatg ctgaaaccag tatccctgcc
    16681 ctgctaaatg gcgaacactc tgtcctttac cgtacccgtt acgatcaaca atctgatgcc
    16741 tggattatgc gtcttgcctg atccaaaaag aacccgtcgg catggcgggt tatttgtcct
    16801 ggttattccc ccgttgtaaa atctctccta aacttaacgg tacggcacca cacttcgggg
    16861 atgaaatgtt cgcgctggta ctttttgttt gctacctgga tggcggttgt gaagatattg
    16921 ttgtggatgt ctacaacacg gaacagcagt gtctttattc tatgagcgat caacggatcc
    16981 gccatggcag ttgttttccg attgaggatt ttatagatgg tttctggcaa ccagcacagg
    17041 agtatggtga tttttaatta ttgcagttgc acaagagtca gttagccccc aaagacagca
    17101 ccggtatcaa tataatgcag gttgccaata tccacgcgat gtcgcaacgg tgtatgacca
    17161 aaccagaaat gatcagcacc tgtaattccc tgcccttttg ggcgttcacc taatcgcgag
    17221 cggctccaca agacctgatg caaatcaacg tccttttgcc attcat
//
LOCUS       DAZOYF010000080        17041 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZOYF010000080 DAZOYF010000000
VERSION     DAZOYF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.080"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..510
                     /locus_tag="ACHUOF_002458"
     CDS             <1..510
                     /locus_tag="ACHUOF_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR0611600.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHUOF_002459"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHUOF_002459"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR0611601.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHUOF_002460"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHUOF_002460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR0611602.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="ACHUOF_002461"
     CDS             complement(2878..3075)
                     /locus_tag="ACHUOF_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0611603.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHUOF_002462"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHUOF_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR0611604.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHUOF_002463"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHUOF_002463"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR0611605.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHUOF_002464"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHUOF_002464"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR0611606.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHUOF_002465"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHUOF_002465"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR0611607.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHUOF_002466"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHUOF_002466"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR0611608.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHUOF_002467"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHUOF_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="ACHUOF_002468"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="ACHUOF_002468"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR0611609.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHUOF_002469"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHUOF_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR0611610.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="ACHUOF_002470"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="ACHUOF_002470"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR0611611.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="ACHUOF_002471"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="ACHUOF_002471"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR0611612.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="ACHUOF_002472"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="ACHUOF_002472"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0611613.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUOF_002473"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUOF_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
BASE COUNT         3967 a         4856 c         4442 g         3776 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcgtaggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc ctcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct agcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgcgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 c
//
LOCUS       DAZOYF010000081        16856 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZOYF010000081 DAZOYF010000000
VERSION     DAZOYF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.081"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..881
                     /locus_tag="ACHUOF_002474"
     CDS             <1..881
                     /locus_tag="ACHUOF_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0611614.1"
                     /translation="DLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSV
                     QLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSY
                     RMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELGE
                     QSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELI
                     SIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVSP
                     AMHIFIQCVEECFVVA"
     gene            complement(916..2250)
                     /gene="srmB"
                     /locus_tag="ACHUOF_002475"
     CDS             complement(916..2250)
                     /gene="srmB"
                     /locus_tag="ACHUOF_002475"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR0611615.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            2382..3119
                     /gene="trmN"
                     /locus_tag="ACHUOF_002476"
     CDS             2382..3119
                     /gene="trmN"
                     /locus_tag="ACHUOF_002476"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR0611616.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(3104..4726)
                     /gene="nadB"
                     /locus_tag="ACHUOF_002477"
                     /pseudo
     CDS             complement(3104..4726)
                     /gene="nadB"
                     /locus_tag="ACHUOF_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            4982..5137
                     /gene="rseD"
                     /locus_tag="ACHUOF_002478"
     CDS             4982..5137
                     /gene="rseD"
                     /locus_tag="ACHUOF_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR0611617.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            5134..5709
                     /gene="rpoE"
                     /locus_tag="ACHUOF_002479"
     CDS             5134..5709
                     /gene="rpoE"
                     /locus_tag="ACHUOF_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR0611618.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            5742..6392
                     /gene="rseA"
                     /locus_tag="ACHUOF_002480"
     CDS             5742..6392
                     /gene="rseA"
                     /locus_tag="ACHUOF_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR0611619.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            6392..7348
                     /gene="rseB"
                     /locus_tag="ACHUOF_002481"
     CDS             6392..7348
                     /gene="rseB"
                     /locus_tag="ACHUOF_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR0611620.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            7345..7824
                     /gene="rseC"
                     /locus_tag="ACHUOF_002482"
     CDS             7345..7824
                     /gene="rseC"
                     /locus_tag="ACHUOF_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR0611621.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            8022..9821
                     /gene="lepA"
                     /locus_tag="ACHUOF_002483"
     CDS             8022..9821
                     /gene="lepA"
                     /locus_tag="ACHUOF_002483"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR0611622.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            9837..10811
                     /gene="lepB"
                     /locus_tag="ACHUOF_002484"
     CDS             9837..10811
                     /gene="lepB"
                     /locus_tag="ACHUOF_002484"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR0611623.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            11083..11763
                     /gene="rnc"
                     /locus_tag="ACHUOF_002485"
     CDS             11083..11763
                     /gene="rnc"
                     /locus_tag="ACHUOF_002485"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR0611624.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            11760..12665
                     /gene="era"
                     /locus_tag="ACHUOF_002486"
     CDS             11760..12665
                     /gene="era"
                     /locus_tag="ACHUOF_002486"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR0611625.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            12677..13405
                     /gene="recO"
                     /locus_tag="ACHUOF_002487"
     CDS             12677..13405
                     /gene="recO"
                     /locus_tag="ACHUOF_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR0611626.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            13417..14148
                     /gene="pdxJ"
                     /locus_tag="ACHUOF_002488"
     CDS             13417..14148
                     /gene="pdxJ"
                     /locus_tag="ACHUOF_002488"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR0611627.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            14148..14528
                     /gene="acpS"
                     /locus_tag="ACHUOF_002489"
     CDS             14148..14528
                     /gene="acpS"
                     /locus_tag="ACHUOF_002489"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR0611628.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            14948..15028
                     /gene="shoB"
                     /locus_tag="ACHUOF_002490"
     CDS             14948..15028
                     /gene="shoB"
                     /locus_tag="ACHUOF_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR0611629.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(15222..15482)
                     /gene="yfhL"
                     /locus_tag="ACHUOF_002491"
     CDS             complement(15222..15482)
                     /gene="yfhL"
                     /locus_tag="ACHUOF_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR0611630.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            15697..16793
                     /locus_tag="ACHUOF_002492"
                     /pseudo
     CDS             15697..16793
                     /locus_tag="ACHUOF_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         4131 a         4142 c         4481 g         4102 t
ORIGIN      
        1 tggatctgcg ccgttttatt acgcttaaaa ccgtggtgga agagggttcc tttttgcgag
       61 cttcgcaaaa attgtgctgt acacaatcga cggtgacttt tcatattcag cagcttgagc
      121 aggagttttc agtccagtta tttgagaaaa ttggccgacg gatgtgcctt acccgcgaag
      181 gaaaaaagtt gctgccgcac atttatgaac ttacccgagt gatggatacg cttcgtgagg
      241 ctgccaaaaa ggagtcagat ccggacgggg aactacgcgt tgtttcgggc gaaacgttgc
      301 tctcttaccg aatgccgcag gtgttgcagc gttttcggca acgcgcacca aaagtgcgtt
      361 tatcgttgca ggcgctgaac tgttacgtga tccgtgatgc gctactgaat gatgaggccg
      421 atgtcggcgt cttttatcgc gtagggaatg atgatgccct gaaccgacga gagttgggtg
      481 aacaatcgct ggtgctggtg gcttcaccac aaattgcaga tgtcgatttt actgagccgg
      541 gaagacataa cgcctgtagc tttattatca acgaaccgca atgtgtcttc cggcagatat
      601 ttgagagcac gctgcgacag cggcggatca cggtggaaaa caccattgag cttataagta
      661 tcgaaagcat caagcgttgt gtcgcggcga atatcggtgt cagttacttg ccccactttg
      721 cggtggaaaa agagattgag agcggagaat taattgaatt gccttttggc gaacagtcgc
      781 agaccattac ggcaatgtgc gcacattatg ctggaaaagc ggttagcccg gcgatgcaca
      841 tttttattca gtgtgttgaa gagtgttttg tggttgcgta aaaaaacaat gccgggctta
      901 aacccggcat tgagattact cttctgtcgt ttgcggtggc acgcccgttc cgctcggttt
      961 acggcgctta ccaatatttt tggtgtcgcg atggcgtttt ttcacccgcg gcttctcttt
     1021 ctctttagct tttttcttct cggcgcgttt tgccagtact ttcttcgatg gcttgccggt
     1081 ctgcttctcg cttggcgcac gcgttttcgg gcgtaactca tcaataacgc gagctttaat
     1141 cggctcttca atgtaacggc caaccttgcc cagcagcaaa tggtcatggg cttccaccag
     1201 cgaaattgcg gtgcctttac gaccggcgcg cgcggtacgt ccgatacgat gcaaataagt
     1261 atcgccactg cgcggcatat cgaagttaaa gacgtggctg acgtcaggaa tgtcgatacc
     1321 gcgcgcggca acatcggtgg caaccagtac gtttacgcgg ccttcggtca ggcgtttaat
     1381 tgcttcgtta cgcttgcctt gtaccatctc accttcgaga tagcagttgt tgatgcccgc
     1441 ttcgcgcagc cagtttgcca gctcatgcac acgttcacgc ttacgcacaa acacgattga
     1501 gcgagtcgct tcaggctgtt ttaacaggtg caccagcaat gcggttttat gttccaggtc
     1561 atcggcgcgg taataccact gatgaatttt tttgcgctca cgggtggacg gattggcaga
     1621 aacttccacc ggatcttcca gcagacgctc ggcaaagtcc tgaatcgcat cgccttccag
     1681 cgttgccgaa aagagcaggg tctgtttacg ccagcgagtt tcgccagcaa tatgttcgat
     1741 atcctgagcg aagcccatat ccagcatacg gtctgcttcg tcgaggatca gcgtttcaac
     1801 cgcgcggcaa tcgaagttct cttcttttat gtattgcagc agacgtccgg tcgtggcgac
     1861 cacgatgtcc tgattttcgc tgaacacttc cgcgtggttc atataggcta cgccgccggt
     1921 gatggtggcg atatccagat gcgtatgttt cgccagttcg cgggcatgat cggccacctg
     1981 catcgccagc tcgcgagttg gggtgaggat caaaatacgc ggcggaccgg atttcttacg
     2041 tgggaaatcg agcaggtgct gcaacgctgg cagcagatac gccgccgttt taccggtgcc
     2101 tgtcggcgca gaaccgagta catcacggcc atcgagcgca ggcggaatgg cggcagcctg
     2161 aatggcggtc gggcgagtga aacctttatc ctggagggct tccagcaggc tttcgtcgag
     2221 ttcaagttcg gaaaaagtcg ttacagtcat gttctacctc tgtgtggggc gctgattata
     2281 gacgttacgg ctgcaatctt catctgtttg tatggatatc gcttctcggg tattgttctc
     2341 acccggtgct atcgccgttt ttcctgcaag gttttatttt catgtcacag tctacatccg
     2401 tgcttcgtcg taatggattt acttttaaac agttttttgt tgctcacgat cgctgtgcga
     2461 tgaaagtggg aacggatggc attttgctgg gcgcatgggc accggtggct ggggtgaaac
     2521 gttgccttga tatcggcgcg ggtagcgggt tgctggcact gatgctggcg cagcgaacca
     2581 gtgacagcgt gataattgat gccgttgagc tggaaagtga agctgccgcg caggcgcagg
     2641 aaaatatcaa ccagtccccg tgggcagagc ggattaacgt ccatacggcg gatattcagc
     2701 agtggatcac acagcagaca gtacgcttcg atttaatcat cagtaaccca ccttactatc
     2761 agcagggagt agagtgcgcg acacctcaac gggaacaggc tcgctatacc actacgcttg
     2821 atcacccatc gctgctgacc tgtgcggcgg agtgcattac cgaagaggga tttttctgcg
     2881 tggtgctgcc agagcagatt ggtaatggtt ttactgaact ggcattaagc atgggctggc
     2941 atttacgttt gcggacggat gtggcggaaa acgaagcgcg actgccgcat cgggtgctgc
     3001 tggcattctc cccgcaggcg ggagaatgct ttagcgatcg cttagtcatt cgtgggccag
     3061 accagaacta ttccgaagcg tatacggcgc tgacccaggc tttttatctg tttatgtaat
     3121 gattgccggg ggaaaggatc gatggaccgg aatgcgtgag cagttccggg taatccagcg
     3181 taaaatgcag cccccggctt tctttgcgca tcatcgcgca gcgaacaatc aactcggcaa
     3241 cctgtaccag attacgcagc tccagcaaat tatttgagac gcggaaatgg gcgtaatatt
     3301 cgtctatttc ttgttggagc atggttatcc gccgcagggc gcgttccagg cgcttcgttg
     3361 tgcgcacaat gccaacgtaa tcccacataa acagccgtag ctcgtgccag ttatgctgaa
     3421 ttactacccg ttcgtcaggg ttctcaacgc ggctttcatc ccacggcggt aacgtactga
     3481 cgccgtgggc ataaggcata cgtctggtga tatcttctgc cgccgaccag ccatagacca
     3541 gacactccag caatgaattc gaggccatgc ggttagcgcc gtgtaagccg gtataactca
     3601 cctcgccaat ggcatacaag ccctcgacgt ccgtacgccc atgatcatca accattacac
     3661 cacctcaggt atagtgtgca gcaggcacaa tcggtaccgg ttcttgtgtg agatcaatcc
     3721 ccagcccgag caatttttca taaatcatcg ggaaatgctg gcgaataaaa tcggcgggct
     3781 tatggctgat gtcgaggaac atacaatctg cgccgaggcg tttcatttca tggtcaatgg
     3841 cgcgggcgac aatatcgcgc ggggccagtt cgccgcgctc atcaaaatcg ggcataaaac
     3901 gcgtgccatc cgggcgcttg agataagcgc cttcgccgcg cagtgcttcc gttaacagga
     3961 aattgcgtgc ctgtgggtga tacagtgcgg tagggtggaa ctgattaaat tcgagattgg
     4021 caacccgaca gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat
     4081 tggtggtgta ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt
     4141 ggcaggtttc caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg
     4201 tgcccggcag gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca
     4261 cacgaatatt cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg
     4321 tggcgtcggc ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt
     4381 agctttcttc gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc
     4441 atggcacaca ggaacgagca ttgctggcga caaattcaac tgcatggtga tcgcaaatac
     4501 cagccccggc aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca
     4561 cggcggcaat accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta
     4621 gaacgatgac ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc
     4681 taccaataat caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat
     4741 ttactaaaca ttgtttggtc ggcatagcat catgttgtgc gaataaacac ctgctatttt
     4801 aatatttgtt acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact
     4861 ggctggtgga ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta
     4921 aatgggcatt tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat
     4981 aatgatccgt ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg
     5041 agacactcta accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc
     5101 gtggaaattt ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc
     5161 ctggttgaac gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat
     5221 cagcataaag tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg
     5281 gtacaagaag cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct
     5341 ttttatacat ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag
     5401 gggcgtcgtc caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc
     5461 ggcgcgttga aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag
     5521 atagttttcc gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg
     5581 gagctggatg gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg
     5641 gtgcgttcac gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc
     5701 aggcgttgac gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt
     5761 ccgctttaat ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc
     5821 cagaaatgca gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata
     5881 ctcccgaggt gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc
     5941 cagtacgtca atcggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaga
     6001 aaatgccatt ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag
     6061 ccgcatgcgt atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa
     6121 cgtcccagca gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc
     6181 cggtaagcct gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac
     6241 aggagcagcg tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc
     6301 actctgaaca gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag
     6361 gaattcaaac tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt
     6421 agtgacaggt agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt
     6481 attacagcag atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat
     6541 caataaacag ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct
     6601 tgcacaattg ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat
     6661 cagctatttt gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc
     6721 tctgccatcg cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc
     6781 cgtcgggcgc acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga
     6841 tggtacacgc tacagctaca tcgtgtggat ggacaccgaa tcgaaattac cgatgcgggt
     6901 tgatctcctt gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattg cttttaacgt
     6961 caatcaggat atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct
     7021 ttctgttcct gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg
     7081 ttttagcgaa gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga
     7141 atcacgtctc tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc
     7201 atcgagcacc gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga
     7261 taacgccgaa atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga
     7321 gaatattaag ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa
     7381 aacgggcagg cgctggtcag ttgtgatgtt aaagcttcat gcagcagctg cgcttcacgc
     7441 gccggttgcg gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc
     7501 gtaccctgtg atgagccgtt agtgccgggg caaaaagtgg agttggggat cgccgaaggc
     7561 agcctgctta gctccgcatt actggtttat atgtcgccgc tggtgggatt attcctcatc
     7621 gcttcgctat ttcagctact ctttgcttct gacgtcgcag cattatgtgg tgcggtcctc
     7681 ggcggtattg gcgggttcct gattgcgcgc ggctactcgc gtaagtttgc tgcccgggca
     7741 gaatggcaac cgatcatctt aagcgtggca ttgccgcctg gccttgtgcg atttgaaaca
     7801 tcttcggaag acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc
     7861 gaggacgttt ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt
     7921 agtgtagaat gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa
     7981 agcctggtta accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact
     8041 tttcgatcat agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga
     8101 tctgcggtgg cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg
     8161 agcgtgagcg tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg
     8221 acggcgaaac ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg
     8281 aagtttcccg ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg
     8341 gcgtagaagc gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg
     8401 tgccggtact gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa
     8461 ttgaagatat cgtcggcatc gacgccaccg atgcagtgcg ctgttcagcg aaaaccggcg
     8521 ttggcgtgca ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc
     8581 cggaaggccc gttgcaggca ctaattatcg actcctggtt cgacaactac ctgggcgttg
     8641 tttcacttat ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga
     8701 gtaccgggca gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc
     8761 gcactgaact gaaatgtggc gaagtaggct ggctcgtatg tgcgattaaa gatatccacg
     8821 gcgctccagt cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg
     8881 gctttaagaa agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact
     8941 atgaagcctt ccgtgacgcg ctgggtaaac tcagcctgaa cgatgcctca ctgttctatg
     9001 agccggaaag ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc
     9061 acatggagat catccaggag cgtctggaac gtgaatacga tctggatctg atcaccactg
     9121 cgccgaccgt agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc
     9181 catccaagct gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc
     9241 acatgctgct gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg
     9301 gcgtgcagac caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga
     9361 tggcggaagt ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt
     9421 ctctggatta caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa
     9481 tcaacggtga acgtgttgat gcgctggcgt tgatcaccca ccgtgataat tcgcaaaacc
     9541 gcggtcgcga gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg
     9601 ccattcaggc agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta
     9661 aaaacgtact ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga
     9721 agcagaaaga aggtaagaaa cgcatgaagc agatcggtaa cgttgagctg ccgcaggaag
     9781 cgttcctcgc cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg
     9841 cgaatatgtt tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg
     9901 tggataaatt ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg
     9961 ccggtgactc actggataaa gcaacgttga aaaaggttgc accgaagcct ggctggctgg
    10021 aaaccggagc ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt
    10081 atgaaccgtt ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta
    10141 ttctggtaga gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg
    10201 aaaccggtca tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc
    10261 ttgattacat caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct
    10321 caaaagagct gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc
    10381 cggtcaccta ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg
    10441 gtggggaagc gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa
    10501 ttcgtctttc cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc
    10561 cgattgcgca ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct
    10621 ggattgttcc gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca
    10681 gccgttactg gggctttgtg cctgaagcga atctggtcgg tcgggccacg gctatctgga
    10741 tgagcttcga taagcaagaa ggcgaatggc cgactggtgt gcgcttaagt cgcattggcg
    10801 gcatccatta atagccatct tcgttcacgt tgtcgccgtt atggcgacaa cgtgaattat
    10861 ttatgagata aatctcccgt ggctaacgac atcccccgtc gttgtgtata gaatattccc
    10921 ccgaagttta aggttggcac cttcaggttg ccacggcaca cgaaacagcg ttggtcccca
    10981 tataccgcta aactgaaact gcagcgaagc agttagcaga gccatgtata tcaggtctgt
    11041 ttcgtgtgct gaattgttga cgcatttatt tattggtatc gcatgaaccc catcgtaatt
    11101 aatcggcttc aacggaagct gggctacact tttaatcatc aggaactgtt gcagcaggca
    11161 ttaactcatc gtagtgccag cagcaaacat aacgagcgtt tagaattttt aggcgactct
    11221 attctgagct acgttatcgc caatgcgctt tatcaccgtt tccctcgtgt ggatgaaggc
    11281 gatatgagcc ggatgcgcgc cacgctggtc cgtggcaata cgctggcgga actggcgcgc
    11341 gaatttgagt taggcgagtg cttacgttta gggccaggtg aacttaaaag cggtggattt
    11401 cgtcgtgagt caattctcgc cgacaccgtc gaagcattaa ttggtggcgt attcctcgac
    11461 agtgatattc aaaccgtcga gaaattaatc cttaactggt atcaaactcg tctggacgaa
    11521 attagcccag gcgataaaca aaaagatccg aaaacgcgct tgcaagaata tttgcagggt
    11581 cgccatctgc cgctgccgac ttatctggta gtccaggtac gtggcgaagc gcacgatcag
    11641 gaatttacta tccactgcca ggtcagcggc ctgagtgaac cggtggttgg cacaggttca
    11701 agccgtcgta aggctgagca ggctgccgcc gaacaggcgt tgaaaaaact ggagctggaa
    11761 tgagcatcga taaaagttac tgcggattta ttgccatcgt cggacgtccg aacgttggca
    11821 aatccacatt gttgaacaaa ctgctggggc agaaaatctc catcacctcc cgcaaggcgc
    11881 agacaactcg tcaccgcatt gtggggatcc atactgaagg cgcgtatcag gcgatctacg
    11941 tcgatacacc gggcctgcat atggaagaaa aacgcgccat taaccgcctg atgaacaaag
    12001 cggcgagcag ctctattggc gatgttgagc tggtgatttt tgtcgttgaa ggcacccgct
    12061 ggacgccgga cgacgaaatg gtgctcaaca aactgcgcga aggcaaagcg ccggtaatcc
    12121 tcgcggtgaa caaagtggac aacgtgcagg agaaagccga tctgctgccg cacctgcagt
    12181 tcctggcaag ccagatgaac ttcctcgata tcgtgccaat ctctgccgaa accgggctga
    12241 atgttgacac tattgcggca atcgtgcgta agcatctgcc tgaagcgact catcacttcc
    12301 cggaagatta catcaccgat cgctcacagc gttttatggc gtctgaaatc atccgcgaaa
    12361 aactgatgcg tttccttggc gctgaactgc cgtactccgt gaccgtggag atcgaacgtt
    12421 tcgtctctaa cgaacgcggc ggttgtgaca tcaacggttt gattctcgtt gagcgtgaag
    12481 ggcagaagaa gatggttatt ggcaacaaag gggccaagat caaaaccatc gggattgaag
    12541 cgcgtaaaga catgcaggaa atgttcgaag cgcctgtcca ccttgaactg tgggtaaaag
    12601 tgaaatcggg ttgggccgac gacgaacgcg cactgcgcag tctcggttat gttgacgatc
    12661 tttaagagcc actccgatgg aaggctggca gcgcgcattt gtcctgcata gtcgcccgtg
    12721 gagcgaaacc agcctgatgc tggacgtctt cacagaggaa tcgggtcgcg tgcgtctggt
    12781 tgccaaaggc gcacgctcta aacgctctac tctgaaaggt gcattacagc ctttcacccc
    12841 tctcttgcta cgttttggcg ggcgtggcga agtcaaaacg ctgcgcagtg ctgaagctgt
    12901 ctcactggcg ctgccattaa gcggtatcac gctttacagc ggtctgtaca tcaacgaact
    12961 tctctcccgc gtactggaat acgagacgcg cttctctgaa ctctttttcg attacttgca
    13021 ctgcattcag tctcttgcag gtgacactgg tacgccagaa cccgcgctgc gtcgctttga
    13081 actggcacta ctcgggcatc tgggctatgg cgtcaatttt acccattgcg cgggtagcgg
    13141 cgagccggta gatggcacca tgacgtatcg ttatcgcgaa gaaaaagggt ttatcgcaag
    13201 cgtcgttatc gacaataaaa cgttcaccgg aaggcagtta aaagcgttaa acgcgcggga
    13261 atttcctgac gcagacacac tacgcgccgc gaaacgcttt acccgaatgg cgcttaagcc
    13321 gtatcttggc ggtaaaccat taaagagcag ggaactgttc cggcagttta tgcctaagcg
    13381 aacggtgaaa acacattatg aatgatgagg attgtcatgg ctgaattact gttaggcgtc
    13441 aacattgacc atattgcaac gctgcgtaac gcacgcggta ctgcttaccc ggatccggtg
    13501 caggcggcgt ttattgccga gcaggcggga gcggacggca ttaccgtgca tttacgtgaa
    13561 gatcgccgcc acattactga ccgcgatgtg cgcatcctgc ggcagacgct ggatacccga
    13621 atgaatctgg agatggcagt gaccgaagag atgctggcga tcgccgttga gacgaagcca
    13681 catttttgct gcttggtacc ggaaaagcgt caggaagtaa caaccgaagg cggcctggac
    13741 gttgcagggc agcgtgaaaa aatacgcgat gcctgcaaac gtctggcaga tgccgggatt
    13801 caggtttctc tgtttatcga cgccgatgaa gagcagatca aagctgcggc agaggttggc
    13861 gcaccgttta tcgagatcca caccggttgc tatgctgatg ccaaaactga cgccgaacag
    13921 gcgcaagagc tggtgcgtat cgccaaagcc gcgacctttg ccgctagtct cggtctgaaa
    13981 gttaacgccg gacacggtct gacctatcac aacgtgaaag ccattgccgc catccctgag
    14041 atgcatgaac tgaatatcgg tcatgccatt attggccgtg cagtgatgac cggactgaaa
    14101 gatgcggtgg cagaaatgaa gcgtctgatg ctggaagcgc gtggctaatg gcaatattag
    14161 gtttaggcac ggatatcgta gagatcgctc gcatagaagc agtgatcgcc cgatccggtg
    14221 agcgcctggc acgccgcgta ttaagcgata acgaatgggc aatctggaaa acgcaccacc
    14281 agccggtgcg ttttctggcg aagcgttttg ctgtgaaaga agccgcagca aaagcgtttg
    14341 gcaccgggat ccgcaatggt ctggcgttta atcaatttga agtattcaat gatgagctcg
    14401 gcaaaccacg gctacggcta tggggtgagg cattaaaact ggcggaaaag ctcggcgttg
    14461 taaatatgca tgtaacgctg gcagatgaac gccactatgc ttgtgccacg gtaattattg
    14521 aaagttaaaa taataccgtt ctctttaaaa agtaaaaccc ccgaactggt aaggaacggg
    14581 ggttttactt tttaacttta attttgtgca gcatgcaccc tcaacaacag cgattatgga
    14641 tcagtgtact tatcttttac aagcaacttg caatctttag cataaaaact cgagccttta
    14701 cgaagaaagc aatattgatg gaaagattaa cgtgaccgcc aattcgtaag tacattaaaa
    14761 ttggcttcgt tattgaagat tttgctgtgc tttacaccat gccacagaat tcccccattg
    14821 aaacgagtgg tgtcgtcaaa gctctggtgt ggagtgcagc atgcaccctc aataactcgc
    14881 acgttcagtt ttggggagat gtaagggcta atctgaatgg ctgcattcct tgtttaagga
    14941 aaaacgaatg actgattgcc gatacctgat taaacgggtc atcaaaatca tcattgctgt
    15001 tttacagctg atccttctgt tcttataaca caaggaaacg tacttaaggt gcgtccggtg
    15061 aaccaatcgg acgcgccttt aataactata aataagtgtc ttggcagata ctatataaat
    15121 taacttagtg aatgattatg ctaatgtcat caattaaata aatataatgg cgttaaggct
    15181 tcccagtaat ataattaata ctctacttcc agagtagaat attaaatttt atccgcgtgg
    15241 tgcatcagca caaatttatc ccacaactgt tcttctgtct cgacatgcgc cggatctttc
    15301 acaatagtat tggggatcgg gcacaccttc tggcaggttg gtgtctcgta gtgccctacg
    15361 cattcggtac acttatcgct gttaatttcg tagatatgat ctcccattga aatcgcctca
    15421 ttcgggcatt cgggttcaca catatcacaa ttaatacagc gtttagtaat tagtaaagac
    15481 atttcaatgg attaccgtta aatcatttta aaatcagtaa gttgtatcga gtttgtatgc
    15541 tttactgtca ttaacttact gtatgttgat ccagtgtatt taaccttgat aaactcagtc
    15601 cagcaacaca aaaccgcaac acattgcatt ttgtcccgta gaaaagactt gtatgtgtga
    15661 gcttgttttc tgcgcctacg cagataagga ttgagaatgc cgcgcactgt aacacataat
    15721 ccggatagcc ccaataatga cgatgtttta gccgcatctg aaaaatggga cgcctgtaaa
    15781 cccccctata ccagcgcaca catgaaaatc tgtgttgctg ccgccaaaat catcctcgct
    15841 gcttccggcg tggctcgctg ttccaaatac gaaaaagaga actatctccg tatcgatttc
    15901 agcaaagccg gtaaggttac attttacgcc gagtttccaa aaaagatggg cctcaaaggt
    15961 aaaaagctcg gcgagtggcc ggagctcgtt atccagctgg cgcgcgaaaa agcactaggt
    16021 atggctgacg gtggactgcg ggcagagtcc gtacatgcag cgctggaaat gtaccgggat
    16081 gacccaaagc caaagtagcc cggcagaagc tgagcccgga cagtttcaca acctacgggg
    16141 tgcgtatcga ccggattaaa gtaacgttcg gcgagcgtga ggtgttcagc gacgtaacat
    16201 acaatcggct ggtggaagtg ctggacgagt ggatcgccac tcgctcgaac aataacgccc
    16261 tggagttgtt tgccgagctc cgtcggttct ggaagttctg cgcacctact ctttgcaacg
    16321 gccgcaatgt tgccgccagt ctgccagatg attatgtttc ctcccgcgta cagaaaccta
    16381 cccccacacg gctttttacc gatattgaat caatcgcccg actctggctc aatgttgctg
    16441 cctgcacctc tgtacaccag aagaatgctg ttcgcttcat gatcatcact ggtgttcgtc
    16501 cgattaatgt ccataacctg cgctgggact acgtttacga ggaggctggt gaaattgttt
    16561 atccggaagg ggttatcggc atgcgagggg ctatgaaaac acaaaaggct ttccgcctgc
    16621 cgataacgcc tgagatccgg cggattatcg acgagcagaa agcctggcgt gattcagttc
    16681 ctgagtgcaa cagggattat gtatttttgc agccacgtga tccaatgcag ccattttcaa
    16741 aacgatcact ggataagctg gtgaaaacat acagcccgga cggggctgta aaatgaggtg
    16801 tactggcaat agcggacact accatttgtt ctttttttaa gcagccatct gatgat
//
LOCUS       DAZOYF010000082        16851 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZOYF010000082 DAZOYF010000000
VERSION     DAZOYF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.082"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..487)
                     /gene="mxiN"
                     /locus_tag="ACHUOF_002493"
     CDS             complement(<1..487)
                     /gene="mxiN"
                     /locus_tag="ACHUOF_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HFR0611631.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KI"
     gene            complement(429..956)
                     /locus_tag="ACHUOF_002494"
     CDS             complement(429..956)
                     /locus_tag="ACHUOF_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFR0611632.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            complement(953..1678)
                     /gene="mxiJ"
                     /locus_tag="ACHUOF_002495"
     CDS             complement(953..1678)
                     /gene="mxiJ"
                     /locus_tag="ACHUOF_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFR0611633.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            complement(1684..1977)
                     /gene="mxiI"
                     /locus_tag="ACHUOF_002496"
     CDS             complement(1684..1977)
                     /gene="mxiI"
                     /locus_tag="ACHUOF_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFR0611634.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(1990..2241)
                     /gene="mxiH"
                     /locus_tag="ACHUOF_002497"
     CDS             complement(1990..2241)
                     /gene="mxiH"
                     /locus_tag="ACHUOF_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HFR0611635.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(2249..3364)
                     /gene="mxiG"
                     /locus_tag="ACHUOF_002498"
     CDS             complement(2249..3364)
                     /gene="mxiG"
                     /locus_tag="ACHUOF_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HFR0611636.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            complement(3373..3831)
                     /gene="ipgF"
                     /locus_tag="ACHUOF_002499"
     CDS             complement(3373..3831)
                     /gene="ipgF"
                     /locus_tag="ACHUOF_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFR0611637.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(3831..4193)
                     /gene="ipgE"
                     /locus_tag="ACHUOF_002500"
     CDS             complement(3831..4193)
                     /gene="ipgE"
                     /locus_tag="ACHUOF_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFR0611638.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(4203..5819)
                     /locus_tag="ACHUOF_002501"
     CDS             complement(4203..5819)
                     /locus_tag="ACHUOF_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HFR0611639.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            6133..7617
                     /gene="icsB"
                     /locus_tag="ACHUOF_002502"
     CDS             6133..7617
                     /gene="icsB"
                     /locus_tag="ACHUOF_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR0611640.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            7630..8019
                     /gene="ipgA"
                     /locus_tag="ACHUOF_002503"
     CDS             7630..8019
                     /gene="ipgA"
                     /locus_tag="ACHUOF_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HFR0611641.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            8035..8661
                     /gene="ipgB1"
                     /locus_tag="ACHUOF_002504"
     CDS             8035..8661
                     /gene="ipgB1"
                     /locus_tag="ACHUOF_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFR0611642.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            8718..9185
                     /gene="ipgC"
                     /locus_tag="ACHUOF_002505"
     CDS             8718..9185
                     /gene="ipgC"
                     /locus_tag="ACHUOF_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFR0611643.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            9191..10933
                     /gene="ipaB"
                     /locus_tag="ACHUOF_002506"
     CDS             9191..10933
                     /gene="ipaB"
                     /locus_tag="ACHUOF_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFR0611644.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            10896..12044
                     /gene="ipaC"
                     /locus_tag="ACHUOF_002507"
     CDS             10896..12044
                     /gene="ipaC"
                     /locus_tag="ACHUOF_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR0611645.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKPTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            12095..13093
                     /gene="ipaD"
                     /locus_tag="ACHUOF_002508"
     CDS             12095..13093
                     /gene="ipaD"
                     /locus_tag="ACHUOF_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFR0611646.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            13102..15003
                     /gene="ipaA"
                     /locus_tag="ACHUOF_002509"
     CDS             13102..15003
                     /gene="ipaA"
                     /locus_tag="ACHUOF_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR0611647.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            15007..15243
                     /locus_tag="ACHUOF_002510"
     CDS             15007..15243
                     /locus_tag="ACHUOF_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFR0611648.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            15460..16389
                     /gene="virB"
                     /locus_tag="ACHUOF_002511"
     CDS             15460..16389
                     /gene="virB"
                     /locus_tag="ACHUOF_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFR0611649.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
BASE COUNT         5886 a         3350 c         2512 g         5103 t
ORIGIN      
        1 taatcttgac atctgagcga tatttatgga gatcaagttc taactttttc ctcagtgcta
       61 aattacactt gggcaaaacc acttttaatt ctgttactgt attacgcaac gaactaagcc
      121 actgctccaa taaagtgcta atgatcctct catcatgtaa attttctgaa agcaaactag
      181 ttatatagtc ctcaatattt cttctatttt cattttgctt taacttccat tcacaaatat
      241 agtcaataat atgctcaaaa gcagtctgta taccaatttg ataaccatca catacagcat
      301 ctatacgcag tgattcagct tttttcgtag cctcctttat aacacaaatt gctttcttct
      361 tactctcagt gtctctatct tttatggttt tacataattc cttttcaatt ctctttataa
      421 caacaccatc ataggcatga tgtctggata ccggtaaagt gcctttttgc atattgcata
      481 ccttcattta atcgggacaa atttctttct acaggcgtat aatttccctc caaagcaatt
      541 ggattaaata ataagtttat tctttcatat ataccaacag atatattttc cttccacgca
      601 ataagatgcc aaaaaccact tcgaatagcg gcctcccctt gcttactgat tttgccccca
      661 taataactaa taaccatctc actatgatta attaaccgag actcttgact accgataaaa
      721 taagcaacag ctggaagtaa attccagttg gcaacaaaca tagcggttac cccgcttaaa
      781 ggttcaatat cgcaatttag atcatattta ttaattatga gattattaat ctcacttctg
      841 attacgccat tttctaacag ttcagaaggc aaattcaacc gatttctatt tatatagaac
      901 gctggatcaa aaattattga aagatatttt ttataaattc catccattct tatcatattt
      961 tgtttctctt gaaccaccct gttttgaatg cccataccaa aagaatgaca actagtacag
     1021 ccatcccgag aaataaatat attacttcat ttgttaaaaa ttccgattta acttccttaa
     1081 caggttgtac atttgtataa acatattctt ctttcggagt taatatgaca gatatatttt
     1141 catacttaac atcagaaaag gtgtttttca aaaatcgctt aatattacta actaatagtt
     1201 cagactcttt cggtgagtca tatatagcga ttactgatat atgcatcggt tttgaagata
     1261 tatttttttc ttcaagatca tagctaacat gtatttttgc cgaaataaca ccaccaatag
     1321 aaactaaaga ctgttccagc cgttgctcaa tagcactata taaacgggcc ttttcagctc
     1381 ttggagaaga cactaatgaa tctgtaggaa acatttgtga gatatcaact ctctccggat
     1441 ttggcaaatc gtacatgcgc atcaaatcaa ctgccgatgc aaatgtcccc ttttcgactt
     1501 gtaccgagat cccctgttta cctccatcaa cttttctagc agtaatatta tggcgttcta
     1561 gcacagatat tatttcattt gcctgtcttt gagataaatt agaaattaac tcttcacgtt
     1621 gctcacatcc aatcaacatc agcaacaaga ataaaataaa acctttatac ctaatcatat
     1681 aaattaagac tttaataaag tttcaacagc cgaaactgtt tttcttgcta acgtgccagc
     1741 taatgatact cctattgaat aatttgagag cgtcgtctgt aacttggcca aagattctgg
     1801 gtttaatgat tctggattgc ttaccatctc cattatttgt gatacttgaa tatctgtatc
     1861 catcttaata tcactatatt tagccgacac gacgtcttcc agacttgata tctcttgaga
     1921 ttgaaaatca ctggctttga taatatcaac ctgattgact ggataaatgt aattcatgaa
     1981 tgctccctat tatctgaagt tttgaataat tgcagcatca acatccttaa tcactttcac
     2041 tgtattggat tgcgcgttcc tatataatgt atattcagat aatttacttt ggtattcagc
     2101 cagcaactgt ggattcgaag gatttttagc taatttatct agtgccaatg ttagttcacc
     2161 ttgtaatgtt tgagttccat catcaaaagt ttcagataat gaactcaatg tccaatcatc
     2221 attcggtact gtaacactca ttttatcctc acttattttt atctaaaaaa aaccagtgtt
     2281 tatcattcaa cattacataa ctgtctttgc tgttaagata tgatttacct ttaaaatcat
     2341 catctatcaa aagtacagaa aactcaatat actgatctcc atatatattt ttatgttcag
     2401 aaatcagtct attaatgagc tcaagctttt catctgtcag ttcttctctg acagaatatg
     2461 taactttatt attttcacaa atctctttat actggacatt tacctttgtg aaaatacctt
     2521 tagcatcccc ccttgcatta tgatcgctaa gatagttgaa tttaatattt ttgatatacg
     2581 gaaattcaac aatcagagca tttttcaatt tatcaagttc ggtatctttt gaactatttc
     2641 tttctttact taaaaacacc agtaattccg ctttatcact cactaaattc aacacataca
     2701 aattgataaa tgggaatcgt tgattgatat attgttgaat tctatttatt tctttgtttg
     2761 acaccagaat atatctgcat ttggtaaatc cggttttatt taaggctaca gacgcccata
     2821 ccgcatcatt ttgtgtttcg gcaagaatat aattgcactg tccagtgttg actattccat
     2881 atctcttatc atctataatt tcagcaattt cttttacttc atctttcttt aaagagaggg
     2941 aaaaaattat aattaacaca acaataacag caaagaaaaa aaacaccgag tggttcttat
     3001 acattccgtt tagtatatgg cctctcgatt tatcctccct catattttta aggtgaaaag
     3061 aaatcccgtc atattcaaat gaattaaatt gtgaattgat atcaatttgc tcaccttttt
     3121 catcactaaa ttgacagata ttatctttgc ttatggtcaa ataaattata ccatccgatt
     3181 tactatctgt tacaggaata atattttctg ggaaattgtc gtttccaaac tcaagtgttt
     3241 ctatcgtccg tccgagaaca attaagttat tcccataata taacggaaac tcatccccga
     3301 caccgtttgt gagtttcact aataacctga atggtgcaag atttgagttc tttgcctcag
     3361 acataaatcc tcttatatcc ttcgattatt ctgcttgctc tctgctgcta tccgcaaata
     3421 gcgtctatag atatcctctg cgtactttaa tctttctttt ttctttttcg gagacgttcc
     3481 tgcattatag gctccaacag cctcccaccc tcttccatac atcatcataa actcatttaa
     3541 taactttgct gcataatgta cagaaagaca tggatgacta attagcattt cctcagaata
     3601 tcccatttct ctaagtcttt tagaatgaaa gtcatttatt tgcattatac cataatcttt
     3661 acttccattg ttattaacat tgactgcaga tttattaaat ccggattctt tttccgcaat
     3721 cgcttttaac aaacttgatg gaatattgta cctttcacca gccttatccc aacaatcagc
     3781 tctccctaaa tatgggataa aacacagcaa aataaatacg aaacgggaca ttaatacccc
     3841 ttcattcttc gcgcaaattc atccttcagc caacgcacac gcgaaatata atattctgta
     3901 ttaacatata catcctcgaa ttcatttatc ccggtaagat ctaatcgcgc aattagattt
     3961 ccgccttcat catcagtagc taagcatatc ttttctgagt aatttaggct caacgcatat
     4021 ataagatcat ttatattttc aggcaatgca caaaaagggc ataataaatt tattgaatct
     4081 ccttcttttt caatatatat tagcacatca tcatcaagca tgattgcttg atcatcgata
     4141 tcaattaaag ggatacccaa ggctcggcaa ataacatctg ctaaatcttc catatattcc
     4201 tcttatacaa atgacgaata ccctttcacc atattccata tttttgggtc ccctattctt
     4261 tcagaataag atagctctaa cgaagatagc ggcaattttt tcatgacttt atttccgggc
     4321 acaccagtat tcatctcttg gatttccata ttaccactat tcattagaat agtagaaaat
     4381 aatcttttct cttctgagga taatttacta tttaattgag aaaactgacc tgtttcatgt
     4441 tttcttatta tttctctttt aatttcagca tcctgcatac ctgttctgtc cttcccactc
     4501 ttacaattcc agcaaggtac agcacctata gtataagcca atagagtcat tctttgtgac
     4561 aatttatacg gctctccatt atcatttttt tgtaactttt tggttacaat ctcttttatc
     4621 tggttagcca gatatatcac atcattctta catggtggat ttttttctat tgcttctgca
     4681 gcccaacctc caatcacacc atttttgagg aaattatccc ccaacaaaga acatattgat
     4741 tcatcattaa gcttgtcaac attcctccag cctaagccca tttttagcgc taattcattt
     4801 acaccaaagt taaatgtcac cacttttaga ttaacgctta cttctttcag aagaccatca
     4861 ctattccgaa ttaataattt cgttggcttc ccccttttag agtttaaccc ttttaatgcg
     4921 ttcacctgat cttttaacat actctcctct cccccggtta aactggtcgg cgtcagaaga
     4981 gaagtcgaaa caatctttaa atctactgtt ttaccagaca aagcctgtga taataactct
     5041 ggtctgctat ataatgctgc gcttactaac tcctcagctt tattacgagc agcaacagct
     5101 ctttcagatg agtttttttt caatccatag gctgatataa ctccatgtct gatacccgaa
     5161 aaaatttcct tcccctcatc atccaccact tttgaaagcc acatattcgc gatatgatca
     5221 ctctctctcg tagaagcaca acaaattcct tttccattat actctttgac aaatatattt
     5281 ttattcccta tcttcatatg agaggcagga gttaattgaa aaccataatt tttcccatga
     5341 tgactaatat tcttgtcgac cggtccccaa ttcttattat ttaaataatc agtaaatatt
     5401 tgttttattt tcgtttgcaa ttccttttta tttttaatgc ctagctgtgc tcttatctct
     5461 tcacttatta ctttattaag ctcattaata tgtcttttat taacttcacc aagtttatag
     5521 gtatctaatc gttccatcat ggctttaatt tttgacgtat caccacctgc caactttatt
     5581 gcctcgcaaa aaacttcagc tctgtcacgc gcaaacaaaa ggctttttcc tgttaagctt
     5641 gtttggttct gtaaatataa tcgattaagc gcttcgatcc ccttatttct ttctccattt
     5701 ttaactcttt gatatgaaat cacgcttaca cctttgtgct ttccggtttc ttctgcgcct
     5761 ttataggaat ctccgctttg aaatgaaacc tgatgcaatc ccaaattagt tatgtgcatt
     5821 taattatcct cagtcataaa tggggcattg attgcagttt tgtacctcgt tttacaacaa
     5881 aaaagatact tattcaacag ggttctctct ttttctataa cactccgagc ctgatacatc
     5941 aggcacttta tcttgtggga tttcatgatg aaacgagcac tacatatgct cacgaggtac
     6001 atattatgta cagcaaaaga aaatttgatt atgttaacag gctcaagttt ctaaatttat
     6061 taattgaata tattatatac caaatacatt ttacatcccc acaatcacca agtaatggag
     6121 agttaataaa gtatgatcct caaaattagc aatttcattg acgcaagcaa tacaaaaggg
     6181 cctatacgcg ttgaagatac agagcatgga cctatattga tagcacagaa attcaaccta
     6241 aaggatctct ttttcagaac attaagcact attaatgcaa agataaattc acagatatta
     6301 aatgaacaac taaaaaatta tcgactagag aatcaaaaat ccttattgct tttcctcaat
     6361 acattagcta gcgaaaaatc tgcagaaagt gcgtttgccg cctatgaagc agctaaaaac
     6421 tcaattcaac actctttcac aggtagagac atcaaattaa tgttgaatac cgcagagcgt
     6481 tttcatggca tcggtacagc caaaaatctt gaaagacatt tagtttttcg ttgctgggga
     6541 aatagaggca taacccattt agggcatact agtatcagta taaaaaacaa tttactacag
     6601 gaaccaactc atacctatct cagctggtat cccggcggga atgttacaaa agatacagaa
     6661 ataaactacc tttttgaaaa acgctcaggt tacagtgtcg atacctataa gcaagacaaa
     6721 ctaaatatga tttcagacca aacagccgaa agacttgatg caggccaaga ggttagaaac
     6781 ttattaaatt caaaacaaga tcaaaataac aacaaaaaaa tattttttcc cagagctaat
     6841 caaaaaaaag acccctatgg ttattggggg gtcagtgctg ataaagtata cattccatta
     6901 tcaggtgata ataaaacaaa ggatggtaaa atcagccata acctttttgg ccttgatgaa
     6961 acaaacatgt caaaatttat atgcaaaaag aaagcagatg ctttcagaca attggcaaat
     7021 tataaattaa tcagtaaatc tgaaaactgt gctggtatgg cacttaatgt tcttaaagca
     7081 ggaaattctg aaatatactt tccactccct gacgttaaat tggttgctac acctaacgat
     7141 gtatacgcgt atgcaaataa agttaggcaa cgcattgaaa gtctcaatca gtcttataac
     7201 gaaataatga agtatattga atctgatttt gatctttcca gattgactca attacgacgc
     7261 agctatctta aaagtttcaa taaaattaac cttatccaca caccaaaaac attcaagcct
     7321 ttatcaattt cactttacaa acatcctact gaaaatgtat cttcagaaga ctttgatgcg
     7381 gtcatcaacg cttgccactc atacttagta aaatcagcac ccagcaatat gactcgtgta
     7441 ttaaacgaat taaaaaccga agcaacagat aaaaaagaag aaatcatcga aaaatcaata
     7501 aaaattattg attattacaa ttctcttaaa tcgccagatc ttggaaccaa actttacatt
     7561 cacgacttat tacagatcaa taagctttta ttgaataact ctcattctaa tatatagaag
     7621 gccatagaaa tgtgtcgcaa actatatgat aaactttatg aaataacagg tgcaaagctt
     7681 gattttaacg ataaaaatca agcctttata ttgctcgaag aacaaatacc tgtttgtata
     7741 acagataatg atgaatacat ctttttgacc ggacttctta atgaacatga actttttaca
     7801 gaaaatatta ttaatccaga acacatactc atccttaact actctttgag tagagattat
     7861 ggttcttcta tctgtcttct ccctgatact catcaatgtg ttttgaccaa gaaacactac
     7921 aaaaaatatc tttcacctga tgaactcatt gaatctttgt atgaattttt attttgtatt
     7981 aaactaacca ttgcaaacat cacttcagaa gtgaactaac atataggggg tatcatgcaa
     8041 attctaaaca aaatacttcc acaggttgaa ttcgcgattc ctcgcccatc atttgattcg
     8101 ttaagtcgca ataagcttgt aaagaaaata ttatctgttt ttaacttaaa acaacgcttt
     8161 cctcagaaga attttggatg cccggtgaat ataaataaaa tcagggacag cgtcatagac
     8221 aaaataaaag atagtaattc ggggaatcaa ctattttgtt ggatgagcca agagcgaacc
     8281 acttatgtct cttcaatgat aaacagaagt atagatgaaa tggccataca caatggagtt
     8341 gttttgacct cagataacaa aaaaaacata tttgcagcta ttgaaaaaaa attcccggat
     8401 ataaaacttg atgaaaaatc agcacaaaca tctatcagtc atacagcact aaacgagatt
     8461 gcctcatctg gcctcagagc aaagatttta aaacgctata gcagtgacat ggatttgttt
     8521 aacacacaaa tgaaggatct tacaaatcta gtatccagtt ccgtctacga taaaatattt
     8581 aatgaatcaa caaaagtttt acaaatagaa ataagcgcag aggttctaaa ggctgtatac
     8641 cgtcaaagca atacaaatta aatcttttat attaaatctt atacttatta aagtaaagca
     8701 gacaaaaagg agaccttatg tctttaaata tcaccgaaaa tgaaagcatc tctactgcag
     8761 taattgatgc aattaactct ggcgctacac tgaaagatat taatgcaatt cctgatgata
     8821 tgatggatga catttattca tatgcttatg acttttacaa caaaggaaga atagaggaag
     8881 ctgaagtttt cttcaggttt ttatgtatat acgactttta caatgtagac tacattatgg
     8941 gactcgcagc tatttatcag ataaaagaac agttccaaca agcagcagac ctttatgctg
     9001 tcgcttttgc attaggaaaa aatgactata caccagtatt ccatactgga caatgccagc
     9061 ttcggttgaa agccccctta aaagctaaag agtgcttcga actcgtaatt caacacagca
     9121 atgatgaaaa attaaaaata aaagcacaat catacttgga cgcaattcag gatatcaagg
     9181 agtaattatt atgcataatg taagcaccac aaccactggt tttcctcttg ccaaaatatt
     9241 ggcttccact gagcttggag acaatactat ccaagctgca aatgatgcag ctaacaaatt
     9301 attttctctt acaattgctg atcttactgc taaccaaaat attaatacaa ctaatgcaca
     9361 ctcaacttca aatatattaa tccctgaact taaagcacca aagtcattaa atgcaagttc
     9421 ccaactaacg cttttaattg gaaaccttat tcaaatactc ggtgaaaaat ctttaactgc
     9481 attaacaaat aaaattactg cttggaagtc ccagcaacag gcaagacagc aaaaaaacct
     9541 agaattctcc gataaaatta acactcttct atctgaaact gaaggactaa ccagagacta
     9601 tgaaaaacaa attaataaac taaaaaacgc agattctaaa ataaaagacc tagaaaataa
     9661 aattaaccaa attcaaacaa gattatccga actcgaccca gagtcaccag aaaagaaaaa
     9721 attaagccgg gaagaaatac aactcactat caaaaaagac gcagcagtta aagacaggac
     9781 attgattgag cagaaaaccc tgtcaattca tagcaaactt acagataaat caatgcaact
     9841 cgaaaaagaa atagactctt tttctgcatt ttcaaacaca gcatctgctg aacagctatc
     9901 aacccagcag aaatcattaa ccggacttgc cagtgttact caattgatgg caacctttat
     9961 tcaactagtt ggaaaaaata atgaagaatc tttaaaaaat gatctggctc tattccagtc
    10021 tctccaagaa tcaagaaaaa ctgaaatgga gagaaaatct gatgagtatg ctgctgaagt
    10081 acgtaaagca gaagaactca acagagtaat gggttgtgtt gggaaaatac ttggggcact
    10141 tttaactatc gttagtgttg ttgcagcagc tttttctgga ggagcctctc tagcactggc
    10201 agctgttggt ttagctctta tggttacgga tgctatagta caagcagcga ccggcaattc
    10261 cttcatggaa caagccctga atccgatcat gaaagcagtc attgaaccct taatcaaact
    10321 cctttcagat gcatttacaa aaatgctcga aggcttgggc gtcgactcga aaaaagccaa
    10381 aatgattggc tctattctgg gggcaatcgc aggcgctctt gtcctagttg cagcagtcgt
    10441 tctcgtagcc actgttggta aacaggcagc agcaaaactt gcagaaaata ttggcaaaat
    10501 aataggtaaa accctcacag accttatacc aaagtttctc aagaattttt cttctcaact
    10561 ggacgattta atcactaatg ctgttgccag attaaataaa tttcttggtg cagcgggtga
    10621 tgaagtaata tccaaacaaa ttatttccac ccatttaaac caagcagttt tattaggaga
    10681 aagtgttaac tctgccacac aagcgggagg aagtgtcgct tctgctgttt tccagaacag
    10741 cgcgtcgaca aatctagcag acctgacatt atcgaaatat caagttgaac aactgtcaaa
    10801 atatatcagt gaagcaatag aaaaattcgg ccaattgcag gaagtaattg cagatctatt
    10861 agcctcaatg tccaactctc aggctaatag aactgatgtt gcaaaagcaa ttttgcaaca
    10921 aactactgct tgatacaaat aaggagaatg ttatggaaat tcaaaacaca aaaccaaccc
    10981 agattttata tacagatata tccacaaaac aaactcaaag ttcttccgaa acacaaaaat
    11041 cacaaaatta tcagcagatt gcagcgcata ttccacttaa tgtcggtaaa aatcccgtat
    11101 taacaaccac attaaatgat gatcaacttt taaagttatc agagcaggtt cagcatgatt
    11161 cagaaatcat tgctcgcctt actgacaaaa agatgaaaga tctttcagag atgagtcaca
    11221 cccttactcc agagaacact ctggatattt ccagtctttc ttctaatgct gtttctttaa
    11281 ttattagtgt agccgttcta ctttctgctc tccgcactgc agaaactaaa ttgggctctc
    11341 aattgtcatt gattgcgttc gatgctacaa aatcagctgc agagaacatt gttcggcaag
    11401 gcctggcagc cctatcatca agcattactg gagcagtcac acaagtaggt ataacgggta
    11461 tcggtgccaa aaaaacgcat tcagggatta gcgaccaaaa aggagcctta agaaagaacc
    11521 ttgccactgc tcaatctctt gaaaaagagc ttgcaggttc taaattaggg ttaaataaac
    11581 aaatagatac aaatatcacc tcaccacaaa ctaactctag cacaaaattt ttaggtaaaa
    11641 ataaactggc gccagataat atatccctgt caactgaaca taaaacttct cttagttctc
    11701 ccgatatttc tttgcaggat aaaattgaca cccagagaag aacttacgag ctcaataccc
    11761 tttctgcgca gcaaaaacaa aacattggcc gtgcaacaat ggaaacatca gccgttgctg
    11821 gtaatatatc cacatcagga gggcgttatg catctgctct tgaagaagaa gaacaactaa
    11881 tcagtcaggc cagcagtaaa caagcagagg aagcatccca agtatctaaa gaagcatccc
    11941 aagcgacaaa tcaattaata caaaaattat tgaatataat tgacagcatc aaccaatcaa
    12001 agaattcgac agccagtcag attgctggta acattcgagc ttaatatatc caagagccat
    12061 aataatatat ggctcttcct gtaaggaaat aaccatgaat ataacaactc tgactaatag
    12121 tatttccacc tcatcattca gtccaaacaa taccaacggt tcatcaaccg aaacagttaa
    12181 ttctgatata aaaacaacga ccagttctca tcctgtaagt tcccttacta tgctcaacga
    12241 cacccttcat aatatcagaa caacaaatca ggcattaaag aaagagcttt cacaaaaaac
    12301 gttgactaaa acatcgctag aagaaatagc attacattca tctcagatta gcatggatgt
    12361 aaataaatcc gctcaactat tagatattct ttccaggcac gaatatccaa ttaataaaga
    12421 cgcaagagaa ttattacatt cagctccgaa agaagccgag cttgatggag atcaaatgat
    12481 atctcataga gaactgtggg ctaaaattgc aaactccatc aatgatatta atgaacagta
    12541 tctgaaagta tatgaacatg ccgttagttc atatactcaa atgtatcaag attttagcgc
    12601 tgttctttcc agtcttgccg gctggatctc tcccggaggt aacgacggaa actccgtgaa
    12661 attacaagtc aactcgctta aaaaggcatt ggaagaactc aaggaaaaat ataaagataa
    12721 accgctatat ccagcaaata atactgttag tcaggaacaa gcaaataaat ggcttacaga
    12781 attaggtgga acaatcggca aggtatctca aaaaaacggg ggatatgttg tcagtataaa
    12841 catgacccca atagacaata tgttaaaaag cttagataat ctaggtggaa atggcgaggt
    12901 tgtgctagat aatgcaaaat atcaggcatg gaatgccgga ttctctgccg aagatgaaac
    12961 aatgaaaaat aatcttcaaa ctttagttca aaaatacagt aatgccaata gtatttttga
    13021 taatttagta aaggttttga gtagtacaat aagctcatgt acagatacag ataaactttt
    13081 tctccatttc tgaggtgcgc catgcataat gtaaataata ctcaagcgcc aacattctta
    13141 tataaggcaa cttcaccatc atcaacagaa tacagcgagt taaaaagcaa aatatccgat
    13201 atccatagtt cgcaaacttc tctaaaaaca ccagcatcag tgtctgaaaa agaaaacttt
    13261 gcaacgtctt ttaatcagaa atgtcttgat tttttatttt cttcctcagg gaaagaagat
    13321 gtgttaagaa gcatttattc caactcaatg aatgcgtatg ccaaaagcga gattctcgaa
    13381 ttttcaaatg ttttgtactc cttagtacat caaaatggtc ttaattttga aaacgaaaag
    13441 ggacttcaaa aaattgtcgc acagtattcg gaactaatta taaaagataa attatcccaa
    13501 gattctgcct ttggaccatg gtcggcaaag aataagaaac tccatcaatt acgacaaaac
    13561 attgagcaca gacttgcact attagcacaa caacacacat ctggtgaagc tttatcattg
    13621 ggacaaaaac tcctcaatac tgaagtatca tcatttatca agaataatat tcttgctgaa
    13681 ttaaagttaa gtaatgaaac tgtttcatct ctcaaactag atgatttagt tgacgcacag
    13741 gcaaaacttg cctttgatag tttgcgcaat caacgtaaaa atactattga tagtaaagga
    13801 tttggtatag gtaaactgtc aagagactta aatacagtag ccgtgtttcc tgagctgttg
    13861 agaaaagtcc ttaatgatat tttagaagat ataaaagatt cgcatcctat ccaagatggc
    13921 ctccctacac ctcccgaaga tatgccagat ggcggaccaa cccccggagc caatgagaaa
    13981 acatcccaac ctgtaattca ctatcatata aataatgata atagaactta cgataataga
    14041 gtttttgaca acagagtata tgacaatagc tatcacgaga acccagaaaa tgatgcacag
    14101 tctcctactt ctcagacaaa cgatctatta tcccgtaacg gaaactcatt actaaatcca
    14161 caaagagcac tagttcaaaa agtaacttcc gttctaccac actctatatc agatactgtc
    14221 cagacatttg caaataattc agctttagaa aaggctttca accatactcc agataattcg
    14281 gatggaatag gttcagacct gttaactacg agtagtcaag aaagatctgc aaataactct
    14341 ctttctcggg gacacaggcc tctgaacata cagaactctt caaccacccc ccctctccac
    14401 ccggaaggag tgacaagcag taatgataac tcatcagata caactaaaag tagcgcttct
    14461 ctttctcata gagtagcttc gcaaatcaat aaattcaact caaacactga ttcaaaagta
    14521 cttcagactg attttttatc aagaaatgga gacacatatt taacacggga aacgatattt
    14581 gaagcttcaa aaaaagtaac aaactcccta agtaatctta tatctctcat tggaactaaa
    14641 tcaggaacac aagaacgaga gttacaggaa aaatcaaagg acattacaaa atccacaaca
    14701 gaacatagaa taaacaacaa attaaaagtt acagatgcaa atataagaaa ctacgtaaca
    14761 gaaaccaacg cagatacaat tgataaaaat catgcgatct atgaaaaggc aaaagaagta
    14821 tctagcgccc tcagcaaggt attgtcaaaa attgacgata cctctgcaga attacttaca
    14881 gatgatatat ctgatttaaa aaataacaat gatattacag ctgaaaacaa taatatatat
    14941 aaagcagcaa aagatgtaac cacttcccta tcaaaagtat taaagaatat caataaggat
    15001 taaaatatga taaaagaaaa aatattatca atagtggcct tttgctatgg tatagcgtac
    15061 agcaaactat ctgaagaaac taaatttatt gaagatcttt ctgccgattc tctttctctg
    15121 attgaaatgc tggatatgat tagttttgag ttcaatctac gtatagatga atctacatta
    15181 gaacacatta ttactattgg agatcttatt tctgtagtca aaaatagtac aaaatcaata
    15241 tagtaattga taagcatttt ttcatctatg gagctctcac atcagagctc cacaagaata
    15301 ttattctttt atccaataaa gataaattgc atcaatccag ctattaaaat agtaattttt
    15361 aagactaccg ttgactatca tcaacagtta catacaatct tgctcacact gcatttaact
    15421 tttgtcaata taaatcaata tgaataaaca gggtgtgata tggtggattt gtgcaacgac
    15481 ttgttaagta taaaggaagg ccaaaagaaa gagtttacac tccattctgg taataaagtt
    15541 tcctttatca aagccaagat tcctcataaa aggatccaag atttaacctt cgtcaaccaa
    15601 aaaacgaatg tacgcgatca agaatcccta acagaagaat cattagccga tatcataaaa
    15661 actataaagc tacaacaatt cttccctgta ataggaaggg agattgatgg tagaattgaa
    15721 attctggatg gcactcgtag aagagcatct gcaatatatg caggagcaga tcttgaagtt
    15781 ctatattcaa aagaatatat atctactctt gatgccagaa aactagcaaa cgatatacaa
    15841 acagcaaaag agcatagcat ccgagaactt ggtattggtc ttaattttct gaaagtatca
    15901 gggatgtcct ataaagacat agccaaaaaa gagaatctgt ctcgcgcgaa agtcactcgt
    15961 gcctttcagg cagcaagcgt tccacaggaa ataatatctc tatttccaat cgcgtcagaa
    16021 cttaacttta atgactacaa gatattattc aattattata aaggacttga aaaggctaat
    16081 gaatctctta gttctacact accaatatta aaggaagaaa taaaagacct tgatacaaat
    16141 ttgcccccgg acatatataa aaaagaaatt ttaaacatca taaagaaaag caaaaacaga
    16201 aagcaaaacc cttcgctaaa agttgactcg ttatttattt ctaaagacaa acggacttac
    16261 ataaaaagaa aagagaataa aacaaacaga actctaatat ttacattatc taaaataaac
    16321 aaaacagttc agagagaaat agatgaagct attcgggata taatttctcg ccatctatcg
    16381 tcttcataaa catcgaacac tgatgtaaac tgcccaattc agataataaa aatcttcgtt
    16441 gaacttgact ttcagccggt ggggtaattc cgtcggctat ctgttattca atccccacgc
    16501 actgtgtttc cttaaaatta cggtgaaaac tcataccctg ctgaccacga gaaaattatc
    16561 tggtgaaatt agtaatattc atatcattga aaatgaattg tgcaactaag ggatgcggca
    16621 tgatggtcaa aattttatgt gaatatgagt gactggccga aaatattcag cgtattttta
    16681 atcgtttcaa taaaaacagc gaggattagc agtcaacgct tttactactc aatttttttt
    16741 acatggtgat aaacaaaaaa tccaccatta acagacttcg ttcacagtat caaaaaagct
    16801 ttaagaatat attagcagat ttctcaaata taattactgt tttattctcc t
//
LOCUS       DAZOYF010000083        16440 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZOYF010000083 DAZOYF010000000
VERSION     DAZOYF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.083"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHUOF_002512"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHUOF_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
     gene            complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHUOF_002513"
     CDS             complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHUOF_002513"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR0611650.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHUOF_002514"
     CDS             complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHUOF_002514"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR0611651.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHUOF_002515"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHUOF_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="ACHUOF_002516"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="ACHUOF_002516"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR0611652.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="ACHUOF_002517"
     CDS             complement(5877..6218)
                     /locus_tag="ACHUOF_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR0611653.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHUOF_002518"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHUOF_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR0611654.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHUOF_002519"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHUOF_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR0611655.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="ACHUOF_002520"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="ACHUOF_002520"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR0611656.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="ACHUOF_002521"
     CDS             12897..14240
                     /locus_tag="ACHUOF_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR0611657.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="ACHUOF_002522"
     CDS             complement(14302..14508)
                     /locus_tag="ACHUOF_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR0611658.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="ACHUOF_002523"
     CDS             14833..15387
                     /locus_tag="ACHUOF_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR0611659.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..16388)
                     /locus_tag="ACHUOF_002524"
     CDS             complement(15450..16388)
                     /locus_tag="ACHUOF_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611660.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
BASE COUNT         3936 a         4464 c         3997 g         4043 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg
    16381 ggtaacatat ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt
//
LOCUS       DAZOYF010000084        16415 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZOYF010000084 DAZOYF010000000
VERSION     DAZOYF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.084"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(128..3277)
                     /gene="acrB"
                     /locus_tag="ACHUOF_002525"
     CDS             complement(128..3277)
                     /gene="acrB"
                     /locus_tag="ACHUOF_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR0611661.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3300..4493)
                     /gene="acrA"
                     /locus_tag="ACHUOF_002526"
     CDS             complement(3300..4493)
                     /gene="acrA"
                     /locus_tag="ACHUOF_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR0611662.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4635..5282
                     /gene="acrR"
                     /locus_tag="ACHUOF_002527"
     CDS             4635..5282
                     /gene="acrR"
                     /locus_tag="ACHUOF_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR0611663.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5410..8772
                     /gene="mscK"
                     /locus_tag="ACHUOF_002528"
     CDS             5410..8772
                     /gene="mscK"
                     /locus_tag="ACHUOF_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR0611664.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8984..9145)
                     /gene="rsmS"
                     /locus_tag="ACHUOF_002529"
     CDS             complement(8984..9145)
                     /gene="rsmS"
                     /locus_tag="ACHUOF_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR0611665.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(9159..9686)
                     /gene="priC"
                     /locus_tag="ACHUOF_002530"
     CDS             complement(9159..9686)
                     /gene="priC"
                     /locus_tag="ACHUOF_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR0611666.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9756..10133
                     /locus_tag="ACHUOF_002531"
     CDS             9756..10133
                     /locus_tag="ACHUOF_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR0611667.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10286..10837
                     /gene="apt"
                     /locus_tag="ACHUOF_002532"
     CDS             10286..10837
                     /gene="apt"
                     /locus_tag="ACHUOF_002532"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR0611668.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10966..12891
                     /gene="dnaX"
                     /locus_tag="ACHUOF_002533"
     CDS             10966..12891
                     /gene="dnaX"
                     /locus_tag="ACHUOF_002533"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR0611669.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    12224..12288
                     /gene="dnaX"
                     /locus_tag="ACHUOF_002533"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12944..13273
                     /locus_tag="ACHUOF_002534"
     CDS             12944..13273
                     /locus_tag="ACHUOF_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR0611670.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13273..13878
                     /gene="recR"
                     /locus_tag="ACHUOF_002535"
     CDS             13273..13878
                     /gene="recR"
                     /locus_tag="ACHUOF_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR0611671.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13988..15862
                     /gene="htpG"
                     /locus_tag="ACHUOF_002536"
     CDS             13988..15862
                     /gene="htpG"
                     /locus_tag="ACHUOF_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR0611672.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16043..>16415
                     /locus_tag="ACHUOF_002537"
     CDS             16043..>16415
                     /locus_tag="ACHUOF_002537"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HFR0611673.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRR"
BASE COUNT         3930 a         4316 c         4351 g         3818 t
ORIGIN      
        1 tgaattttat acgtggttaa tactggtttt cgtatgagat cctgagttgg tggttcaatt
       61 actccttaat gttcgtaggt tatgcataaa aaggccgctt acgcggcctt agtgattaca
      121 tgttgtatca atgatgatcg acagtatggc tgtgctcgat atcttcattc ttgcggctaa
      181 agcggcggcg aaccaccaca aagaataccg gaacgaagaa gattgccagt accgttgcgg
      241 tcaccatccc gcccattaca ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag
      301 tactgataac cagcggcata acgccgagga taaacgccag cgaggtcatc aggatcggac
      361 gtaaacgcat ccgcaccgca tcaagcgtcg cttcaatcag acctttacct tctttatcca
      421 tcaagtcttt ggcgaattcg acgataagta tcgcgttctt cgccgacaac ccaatggttg
      481 tgagcagtcc tacttggaag taaacgtcat tggtcaggcc acggaaggtg gcagccagca
      541 acgcaccgat aacccccagc ggaacgacca gcataacgga gaacggagtc gaccagctct
      601 cgtacagcgc cgccagacac aggaatacga caatcaacga aatcgcgtac agtgaaggtg
      661 cctggttgcc ggagagacgt tcctgatagg acatccccgt ccagtcatag ccaacaccgg
      721 taggcagttt gctcgccagt tgttccatca gctccattgc ttcaccggta cttttacccg
      781 gtgccgcctg gcctaagatt tccatggatg gcaggccgtt gtaacgttcc agacgcggcg
      841 aaccgtactc ccaacgagaa gaggagaacg ccgagaatgg caccatctga ccatcagcag
      901 cacgaacata ccagtcgccg atatcatccg gcagcatacg gtatttcgct tctgacatga
      961 cgtaaacttt cttcacacga ccgcggtcga taaagtcgtt cacatagcta ccgccccatg
     1021 cagcgcccag agtggtgtta atgtcgttga tagaaacacc cagcgcctgc gctttttcct
     1081 ggtcgatatc aatcttaaac tgcggggtat cttccagacc gtttggacgt acgctggtca
     1141 gcatatcagg gtgcttcgct gcttctgcaa gcagctggtt acgcgcctga gtcagttttt
     1201 cgtgaccaag gccagcctgg tcaatcagct caaagtcaaa gccggttgca gtacccagtt
     1261 ccacgattgc gggcaggtta aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc
     1321 gtgttgcacg catggtaatc gcttcaactt tgttttcttc gcccggacga tcggcccagt
     1381 ccttcaagga aacgaacgca ataccggtat tctgaccacg tcccgcaaag ccgaagccgt
     1441 taacggcgaa caccgactca acgttgttct tttctttggt cagatagtaa tgcgttacct
     1501 cattgagcac tttctgcgta cgttcctgcg ttgcacctgc tggcagctga accatggtca
     1561 taaatacgcc ctggtcctca tctggcaaga aggagcttgg cagacgcacg aacagatagg
     1621 ccatgccgac cacgatgatc agatacagca ctaggtaacg ccccgtactg cgcagaatac
     1681 cgcctacgct gtcggtgtag tggtgcgtgc tcttctcgaa catgcggttc aaccagccga
     1741 agaagccttt tttaccttcc ccgtgatcgc ctttggcaat cggtttcagc atggtggcac
     1801 aaagagccgg agtcaggatc aacgctacca gtaccgacag cgccattgct gaaacaatgg
     1861 taatagagaa ctgacgatag atagcaccag tagaaccgcc aaagaaggcc atcggtacga
     1921 ataccgccga cagtaccatc gcgataccga ccagagcgcc ctgaatctgc cccatcgact
     1981 tacgggtagc ttcttttggc ggcaaacctt cttccgccat aacacgctcc acgttttcta
     2041 ccacaacgat agcgtcatcc accaacaagc cgatggcgag caccatcccg aacattgtta
     2101 gcgtgtttat cgagaagcca aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg
     2161 gtacggcaat ggtcggaatc aacgtcgcgc ggaagttctg caggaacaga tacataacca
     2221 ggaacacgag gatgatcgct tcgaccagcg ttttaaccac ttcgtgaata gagattttca
     2281 cgaacggcgt ggtgtcgtac gggtaaacaa ttttcagacc cgacgggaag aacggttcca
     2341 tcttcgccag ttcagcacgg attgccgcag cggtatccag cgcgtttgca ccggtcgcca
     2401 gcttgatccc cagaccggaa gcaggttggc cgttaaactc tgcgatgatg tcgtagttct
     2461 caccacccag ctcaatcttc gccacatcac gcagcagtac gcgggaacca tcctgattca
     2521 ctttcagcag gattttgccg aactcttcag tagaggtcag acgcgtctga gcaataatag
     2581 aggcgttaag ctgttggcct ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct
     2641 gggcgttctg cgctttgatg gcggtaatga catcaaccgg cgttagctgg aatttgttca
     2701 gctcattcgg gttcatccag atacgcatcg cgtactgtga accgaacaac tgaacatcac
     2761 ccacgcccga cgtacggctg atggcatctt tcatattcgc cgctacgtag tcagagatat
     2821 cctcctgcgt catggtgcca tcggtgttga taacgccgac aaccatcagg aagctgctgg
     2881 atgatttctc aacgctcacc ccttgctgct gaacttcttg cggcagcaac ggcatcgcca
     2941 gctgcagttt gttctgcacc tgaacctgcg cgatatccgc atcagtacca gactcaaagg
     3001 tcagggtgat ctgcacggta cccgtggagt cactgttaga ggacatgtac atcaggttat
     3061 cgataccgtt catattctgt tcgataacct gtgtcaccgt gtcctgcact gttttcgcat
     3121 cagcgccggg gtaggaggcg gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg
     3181 ccaccggcag tttgaggatc gccagccccc ctgccaacat gatgataatg gcgatcaccc
     3241 acgcaaaaat cgggcgatcg ataaagaaat taggcatgtc ttaacggctc ctgtttaagt
     3301 taagacttgg actgttcagg ctgagcaccg cttgcggctt gctggttatt atcagcggta
     3361 acttcttgtg cttttacctg gacaccagga cgcactttct gcagcccact tattactacg
     3421 cgatcgcctg ctttcagacc ttctgtcacc agccacttat cgccaatagc ctggcttgca
     3481 acgatcggac gggtttccac tttgtcatcc gcgccaacta ccagtacggt ggcatcacca
     3541 cgcggcgtac gggttacgcc ctgttgcggg actaaaatag cgtttggatt aagcccttct
     3601 tccagacgtg cgcgcacgaa catacccggc agcagagtgt gatccgggtt cgggaagata
     3661 gcgcgtaggg tgatagaccc agtggtctga tcaacggtaa cgtcagagaa ttccagcgta
     3721 ccgtcctgcg ggaacttaat gccgtcactg gtgatcagcg acactttggc tttgccgttc
     3781 tcttgtttca gcgtgccatt cgccagttcc tgtttcaggc gaaggaagtc gttgctggac
     3841 tgggtcacat caacgtagat cggatcaagt tgctgcacgg ttgccagcac agtcgcctga
     3901 ccgttctgta ccaatgcgcc ttccgtcacg ttcgacttac caatgcgacc gctaatcgga
     3961 gaggtgactt tggtgtaagc cagattgatc cgcgcagttt caacggcagc tttcgccgca
     4021 gttaccgcag cattcgcctg ttgcgcatca gccagagcct gatcgtaccc ttgcttactg
     4081 atgtactgag taccgagcag tttctgataa cgattcaccg tcaattgcgc gatattggct
     4141 gcagcctggg ctttcgccag atcacctttc gcactgtcgt atgtcgcctg ataggtcgca
     4201 ggatcaatct gatagagaga gacaccagct tcgatgtcgc taccttcttt gaaattacgc
     4261 ttcaggataa tcccgctaac ttgaggacga acttctgcga tccggtaggc actggtgcga
     4321 cctggaagct cggttgtgat ctgcagaggt tcagttttga ctgttactac gccaacggcg
     4381 ggcatctgct ggccaccttg ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg
     4441 cctgagagca tcagaacgac cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa
     4501 cctcgagtgt ccgatttcaa attggtcaat ggtcaaaagt taataaaccc attgctgcgt
     4561 ttatattatc gtcgtgctat ggtacataca ttcacaaatg tatgtaaatc taacgcctgt
     4621 aaattcacga acatatggca cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca
     4681 tcctcgatgt ggctctacgt cttttctcac agcagggggt atcatccacc tcgctgggcg
     4741 agattgcaaa agcagctggc gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt
     4801 cggatttgtt cagtgagatc tgggaactgt cagaatccaa tattggtgaa ctagagcttg
     4861 agtatcaggc aaaattccct ggcgatccac tctcagtatt aagagagata ttaattcatg
     4921 ttcttgaatc cacggtgaca gaagaacgac gtcgattatt gatggagatt atattccaca
     4981 aatgcgaatt tgtcggagaa atggctgttg tgcaacaggc acaacgtaat ctctgtctgg
     5041 aaagttatga ccgtatagaa caaacgttaa aacattgtat tgaagcgaaa atgttgcctg
     5101 cggatttaat gacgcgtcgg gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg
     5161 aaaactggct ctttgccccg caatcttttg atcttaaaaa agaagcccgc gattacgtcg
     5221 ccatcttact ggagatgtat ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat
     5281 aaccctgaat ctgactccag gatttttcct ggacattttc gtcgttgcta ttctggttca
     5341 ctgcgtcgtg atattcttgc ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct
     5401 tcattcatca tgactatgtt ccagtattac aaacgatcac ggcattttgt tttttcagca
     5461 tttattgctt ttgtttttgt cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg
     5521 aatggtgatc tgccgacaaa agcggacctg caggcgcaac ttgactcact aaataaacaa
     5581 aaagatcttt ctgctcagga caaactggtg cagcaggatc tgacagatac attagccacc
     5641 ctcgataaaa tcgatcgcat aaaagaagag acagttcagc tacggcaaaa agtcgctgaa
     5701 gcgccggaaa aaatgcgcca ggcgaccgcg gcgttaacag cacttagcga tgtcgataac
     5761 gacgaagaaa cgcgcaaaat tctgagtacg ctttcgttgc gccagctgga aactcgcgtt
     5821 gcccaggcgc tggacgattt gcaaaacgca caaaacgatc tggcgtctta taacagccag
     5881 ctggtttcgt tacagacgca gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag
     5941 cagctgcaac aaattcgcag tcgtctggat gggactgatg tcggcgagac tgccttacgt
     6001 cccagccaga aagtgttaat gcaggctcag caggcgttgc tgaatgcgga gattgaccag
     6061 cagcgtaaaa gcctggaagg gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat
     6121 tacgtgacgg cgaacagcgc ccgtctggag caccagttgc agctgttgca agaagcggta
     6181 aacagcaagc gcctgacttt aactgaaaaa acggcgcagg aagccgtctc cccggatgaa
     6241 gccgcgcgta ttcaggctaa tccgctggtg aaacaggaac tggaaattaa ccagcagtta
     6301 agtcagcgtc tgattaccgc gactgaaaac ggtaatcagt tgatgcagca aaacattaaa
     6361 gtcaaaaact ggctggagcg ggcgctgcaa tcggaacgaa atattaaaga gcagattgcc
     6421 gtcctgaagg gcagcctgct gttgtctcgt atcctttacc agcaacaaca aacgctgccc
     6481 tcggcggatg aactggaaaa catgaccaac cgcatcgcgg atttgcgtct cgaacagttt
     6541 gaagttaacc agcagcgtga tgcactcttc cagagcgatg cgttcgtcaa caaactggaa
     6601 gaaggtcaca ccaacgaagt caacagcgaa gttcacgatg cgttattgca agtggttgat
     6661 atgcgtcgcg aattgctgga tcaactcaac aaacagttgg gtaaccagct gatgatggcc
     6721 attaacctgc aaatcaacca gcagcagtta atgagcgtat cgaaaaacct gaaatccatc
     6781 ctgactcagc aaatcttttg ggtgaacagt aaccgtccaa tggactggga ctggatcaaa
     6841 gcgttcccgc aaagcctgaa agatgaattt aagtcgatga aaatcacggt gaactgggaa
     6901 aaagcctggc ctgccgtttt tatcgctttc ctcgccggtt tgccgctgct gttgattgcc
     6961 gggctgatcc actggcgtct gggctggctg aaagcgtatc aacagaaact ggcttccgct
     7021 gtgggttccc tgcgtaacga tagccagctc aacacaccaa aagcgatcct tatcgacctg
     7081 atccgcgcgc tgccggtgtg cctgattatt ctcgcggttg gcctgattct gttgaccatg
     7141 cagctcaaca tcagcgaact gctatggtcg ttcagtaaaa aactggcgat tttctggctg
     7201 gtgtttggcc tgtgctggaa ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc
     7261 atgccggagc agcagaccag ccactggcgt cggcaaattg tccgcatcag tctcgcgttg
     7321 ctgcctatcc atttctggtc tgtggtggca gaactttccc cgctgcatct gatggatgat
     7381 gtgctggggc aggcgatgat tttcttcaac ctgctgctga ttgccttcct ggtatggcca
     7441 atgtgccgcg aaagctggcg tgataaagag tcgcacacca tgcgactagt caccattacc
     7501 gtgctgtcga ttatcccgat tgcattgatg gtgctgactg ccacgggtta tttctacacc
     7561 acgctgcgcc tctccgggcg ttggattgaa accgtttatc tggtgatcat ctggaacttg
     7621 ctctaccaga cggtattgcg cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt
     7681 gcgctggcgc gtcggcagaa tctggtgaaa gagggcgcag aaggtgccga accgccggaa
     7741 gaacccacca ttgcactgga gcaagttaac cagcagacgc tgcgtattac catgttgctg
     7801 atgtttgcgc tgttcggtgt catgttctgg gcaatttggt ccgatttgat caccgtgttc
     7861 agctatctcg acagcatcac gctctggcat tacaacggca ctgaagctgg cgctgcggtg
     7921 gtgaaaaacg tcaccatggg cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg
     7981 gcgttgattc gcaacctgcc tggtttactg gaagtgctgg tgctctcgcg actgaatatg
     8041 cgccagggcg cgtcgtatgc cattactacc atccttaact acatcattat tgccgttggt
     8101 gcgatgacgg tgttcggatc gctgggcgtc tcttgggata aactccagtg gctggccgca
     8161 gcattatccg taggtcttgg ttttggttta caagaaattt tcggtaactt cgtctccggt
     8221 ttgatcattc tgttcgaacg tccggtgcgt attggcgata cggtaaccat tggtagcttc
     8281 tcggggacgg taagtaagat ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa
     8341 gaagtgatca tcccgaacaa agcgtttgtt accgagcgtc tgatcaactg gtcgttgact
     8401 gacactacta cgcgtctggt gatccgtctc ggcgtggcct atggctccga tctggaaaaa
     8461 gtgcgtaaag tgttactgaa ggcggcgact gagcacccaa gggtgatgca cgaaccaatg
     8521 ccggaagtct tctttacggc atttggtgcc agcacgttgg atcatgagct gcgtctgtat
     8581 gtgcgtgaac tgcgtgaccg tagccgtact gtcgatgagc tgaaccgtac tatcgatcag
     8641 ctgtgccgtg aaaacgacat caacattgcc tttaaccagc ttgaagtgca tctgcacaac
     8701 gagaagggcg atgaggtgac ggaagtaaaa cgcgactaca aaggcgacga tccgacacca
     8761 gcggttgggt aataaccaaa gggggcgaca tttagttgcc ccgagattac tgacaaagtg
     8821 cgcgttgttc atgccggatg cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat
     8881 tcaatatatt gcaggagcgg tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt
     8941 ttgcccgcaa tcttagggga catttagcgg ccccatttat ttctcacttt tccgcctcat
     9001 catcgtgcgt taatttcttt tcataatcac gctttacaat atccagcgcg cgcagcacgg
     9061 tactggcagg gatctgattt tcctccagca gcacaatcaa atcgacagcc agtttgacat
     9121 cgtcaggggc attttcaagt gacatactct ttccattgct agcgggttaa gcgcgctagc
     9181 ctgttttcga ttttttccag cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc
     9241 acttcacgat gcagcgtttg ctgttccacg agatcggtca cccgcgccag gcgggctctg
     9301 cgttcggcaa ccatctcacg tagccgccgc tcaaaatcct gatgctgaat acgtttacgc
     9361 tgccagcggg aaattttcgg tggcgcactg tcccactcgc gcaatgacca ggcggaggct
     9421 tcacgcgcga tggcttccag ttgtgccgcc agatgttccg ccagccaggc cacttgcggc
     9481 agctgttgct gctcaactgc atgacgaagc gcagccagat tatcgcccgc ctcgtcgaga
     9541 caagactgta gtgttgtcgc acgagtctga aaaagatgcc tgtcgaaacg agcacttagc
     9601 gtggcgaact gtgacaccgg ggcacaacgc tgacgcagcg tagcgagctg tccttccagt
     9661 ttttccagca gcagggcggt tttcacgata agacctcaat gaaaatgata attgttatgc
     9721 taaagtagcc actatgtaag ctgacaacac tcaatatgca acgaatcatt ttaatcatca
     9781 ttggctggct ggcggtagtg ctgggtacgc tgggcgtggt attaccggta ttaccgacga
     9841 cgccgtttat cctgctggcg gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct
     9901 ggttgctgta ccgctcatgg tttggcagct atctacgttt ctggcagaaa catcatgcga
     9961 tgccgcgcgg cgtcaaaccg cgggcgattt tgcttatttt gctcacgttt gccatttctc
    10021 tgtggttcgt ccagatgcca tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc
    10081 ttttctatat gtggcgaatt ccggtgattg atgaaaagca agaaaagcac tgaagcacaa
    10141 caatcgcagt tgcaattatt gcgtacagcc agtacattct ggcgttttcg agcacaggcg
    10201 caggcggtca aaggttaaac aacagttact tttgatacgt ttaaaacgcg ccgtgagtac
    10261 caccgtaaca agcaggcata cacttatgac cgcgactgca cagcagcttg agtatctcaa
    10321 aaatagcatc aaaagcattc aggactaccc aaaacccggc attcttttcc gcgatgtcac
    10381 cagcttactg gaagacccga aagcttacgc tctcagcatc gacttgctgg ttgagcgtta
    10441 caaaaatgcg ggcattacca aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc
    10501 tccggtagct ctgggtctgg gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc
    10561 gcgtgaaacc atcagtgaaa attacgacct ggaatacggc accgatcagc tggaaatcca
    10621 cgttgatgcc atcaaaccgg gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg
    10681 cggcactatc gaagcgaccg ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc
    10741 tgcgttcatt atcaacctgt ttgatctcgg cggcgaacag cgtctcgaaa aacagggcat
    10801 taccagctac agccttgtcc cgttcccggg ccattaatta tcgccagtct tgtgctgccc
    10861 acgctacgga cagcacaaga tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc
    10921 attacccctt cgtgaatcca ccttccagcg tttcagagcc tgccaatgag ttatcaggtt
    10981 ttagcccgaa aatggcgccc acaaaccttt gctgacgtcg tcggccagga acatgtgctg
    11041 accgcactgg cgaacggctt gtcgttaggg cgtattcatc atgcttatct tttttccggc
    11101 acccgtggcg tcggaaaaac ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa
    11161 accggcatta ccgcgacgcc gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg
    11221 cgctttgtcg atctgattga aatcgacgcc gcctcgcgca ccaaagttga agatacccgc
    11281 gacctgctgg ataacgtcca gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc
    11341 gacgaagtgc atatgctgtc gcgccacagc tttaacgcac tgttaaaaac ccttgaagag
    11401 ccgccggagc acgttaagtt tctgctggcg acgaccgatc cacagaaatt gccggtgacg
    11461 attttgtcac gctgtctgca atttcatctc aaggcgctgg atgtcgagca aattcgccat
    11521 cagcttgagc acatcctcaa tgaagaacat atcgctcacg agccgcgggc gctgcaattg
    11581 ttggcgcgcg ccgctgaagg cagcctgcga gatgccttaa gtctgaccga ccaggcgatt
    11641 gccagcggtg acggccaggt ttcaacccag gcggtcagtg cgatgctggg tacgcttgac
    11701 gacgatcagg cgctgtcgct ggttgaagcg atggtcgagg gcaacggcga gcgcgtaatg
    11761 gcactgatta atgaagccgc tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg
    11821 ctcggcttgt tgcatcgtat tgcgatggta caactttcgc ctgctgcact tggcaacgac
    11881 atggccgcca tcgagctgcg gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt
    11941 cagctttact atcagacgct gttgattggt cgcaaagaat taccgtatgc gccggaccgc
    12001 cgcatgggcg ttgagatgac gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg
    12061 cctgagccag aagtgccacg ccagtccttt gcacccgtcg cgccaacggc agtaatgacg
    12121 ccaaccctgg tgccgccgca atcagcgccg cagcaggcac cgactgtacc gctcccggaa
    12181 accaccagcc aggtgctggc ggcgcgccag cagttgcagc gcgtgcaggg agcaaccaaa
    12241 gcaaaaaaga gtgaaccggc agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg
    12301 gaaagactgg cttcggtcac cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa
    12361 aaagcgccag ccaaaaaaga agcgtatcgc tggaaggcga ccactccggt gatgcagcaa
    12421 aaagaagtgg tcgccacgcc gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg
    12481 gaactggcgg tgaagctagc ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg
    12541 agccaacttt cgctaccaaa actggtcgaa caggtggcgt taaatgcctg gaaagaggag
    12601 agcgacaacg cagtatgtct gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt
    12661 gcacagcaaa aactggctga agcgttgagc atgttaaaag gttcaacggt tgaactgact
    12721 atcgttgaag atgataatcc cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac
    12781 gaagaaaaac ttgcgcaggc gcgcgagtcc attattgcgg ataataatat tcagaccctg
    12841 cgtcggtttt tcgatgcgga gctggatgaa gaaagtatcc gccccatttg atcgtaagca
    12901 cagcttacgt tcgtcatcct taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg
    12961 tctgggtaac ctgatgaagc aagcccagca gatgcaagaa aaaatgcaga aaatgcagga
    13021 agagatcgcg cagctggaag ttaccggcga atctggcgca ggtctggtaa aagtgaccat
    13081 caacggtgca cacaactgcc gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa
    13141 agagatgctg gaagacctgg tggctgcagc attcaacgac gcagcacgtc gtattgaaga
    13201 aacgcagaaa gaaaaaatgg cctctgtatc ctccggaatg cagctgccgc ctggctttaa
    13261 gatgccgttc tgatgcaaac cagcccgctg ttaacacagc ttatggaagc actgcgctgt
    13321 ctgccgggcg ttggcccgaa gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat
    13381 cgtagcggcg ggatgcgtct ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac
    13441 tgcgccgatt gccgcacttt caccgaacag gaagtctgta acatctgttc gaatccgcgt
    13501 cgtcaggaaa acggtcaaat ctgtgtggtg gagagtccgg cggacatcta cgccattgag
    13561 cagacggggc agttttctgg tcgttatttt gtgttgatgg ggcatctgtc accgctggac
    13621 ggcatcggtc cggatgatat cgggcttgat cgtctggaac agcgtctggc agaggaaaaa
    13681 atcactgaag tgatcctcgc caccaatccc acggttgaag gtgaagctac cgctaactac
    13741 attgccgagc tttgcgcgca atatgacgtg gaagccagcc gaatcgctca tggcgtaccg
    13801 gttggcggcg aactggaaat ggtcgatggc accacgctgt cacactccct cgccgggcgt
    13861 cataagattc gtttttaagc aaacgagagc aggagcacct gctctcgctt gaaattattc
    13921 tcccttgtcc ccatctctcc ctcatcctgt ttttaacctt aaaatggcat tattgaggta
    13981 gacctacatg aaaggacaag aaactcgtgg ttttcagtca gaagtgaaac agcttctgca
    14041 cctgatgatc cattctctct attccaataa agaaatcttc ctgcgtgagc ttatctctaa
    14101 cgcctccgat gcggcggaca agctgcgttt ccgtgcgcta tctaacccgg acctgtacga
    14161 aggtgatggc gaactgcgcg ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat
    14221 ctccgataac ggcgtgggga tgacccgcga cgaagtgatt gaccatctgg ggactatcgc
    14281 taaatccggt accaaatcat tcctcgaatc cctgggttct gaccaagcga aagacagcca
    14341 gctgatcggt cagtttggtg ttggtttcta ctctgcgttt atcgtggccg acaaagtgac
    14401 cgtgcgtact cgcgcggcag gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg
    14461 cgaaggtgaa tataccgttg ccgatatcac caaagaagat cgtggtactg aaatcaccct
    14521 gcatctgcgt gaaggcgaag acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag
    14581 caaatactcc gaccatatcg cgctgccggt agagatcgaa aaacgcgaag agaaagatgg
    14641 cgaaaccgtt atctcctggg caaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa
    14701 gtcggaaatc accgatgaag agtataaaga gttctacaaa cacatcgccc acgactttaa
    14761 cgatccgctg acctggagcc acaaccgcgt tgaaggtaag caggagtaca ccagcctgct
    14821 gtacatcccg tcccaggctc cgtgggatat gtggaaccgt gatcataaac acggcctgaa
    14881 actgtacgtt caacgcgtgt ttatcatgga cgacgctgaa cagttcatgc cgaactatct
    14941 gcgcttcgtg cgtggtctga ttgactccag cgatctgccg ctgaacgttt cccgtgaaat
    15001 cctccaggac agcacggtaa cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca
    15061 aatgctggaa aaactggcga aagacgacgc ggaaaaatac cagaccttct ggcaacagtt
    15121 tggcctggta ctgaaagaag gtccggcgga agatttcgct aaccaggaag cgatcgccaa
    15181 actgctgcgt tttgcttcta cttataccga ttcttctgcg cagaccgtgt ctctggaaga
    15241 ctacgtttcc cgcatgaaag aagggcagga gaaaatctac tacatcactg cagacagcta
    15301 tgcggcagcg aagagcagcc cgcacctgga actgctgcgt aagaaaggca tcgaagttct
    15361 gctgctttcc gaccgcatcg atgagtggat gatgaactat ctgactgagt tcgacggtaa
    15421 accgttccag tcggtgtcta aagttgacga gtcgctggaa aaactggctg acgaagttga
    15481 tgagagcgcg aaagaagcgg agaaagcact gactccgttc atcgaccgcg tgaaagctct
    15541 gctcggcgag cgcgtgaaag atgtccgtct gactcaccgt ctgaccgata cgccagcgat
    15601 cgtttcgacc gacgcggacg aaatgagcac tcagatggcg aaactgttcg ctgcggcggg
    15661 ccagaaagtg ccagaagtga aatacatctt cgaactgaac ccggatcacg tactggtgaa
    15721 acgtgcggca gatactgaag atgaagccaa gttcagcgag tgggtagaac tgctgctgga
    15781 tcaggcgctg ctggcagaac gcggcacgct ggaagatccg aacctgttta ttcgtcgtat
    15841 gaatcagctg ctggtttcct gatgtcatgc cggatgacct ttgtgtcatc cggcattttt
    15901 cttttcatca tctgtacttt ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg
    15961 cctttcttga ggcaatcgcc tgttggtggt atcgtttatc gctttttcaa aaaaatcgac
    16021 acattttaag gggattttcg caatgcgtat cattctgctt ggcgctccgg gcgcggggaa
    16081 agggactcag gctcagttca tcatggagaa atatggtatt ccgcaaatct ccactggcga
    16141 tatgctgcgt gctgcggtca aatctggctc cgagctgggt aaacaagcaa aagacattat
    16201 ggatgctggc aaactggtca ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc
    16261 tcaggaagac tgccgtaatg gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc
    16321 agacgcgatg aaagaagcgg gcatcaatgt tgattacgtt ctggaattcg acgtaccgga
    16381 cgaactgatt gttgatcgta tcgtaggccg ccgcg
//
LOCUS       DAZOYF010000085        16089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZOYF010000085 DAZOYF010000000
VERSION     DAZOYF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.085"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(1..386)
                     /locus_tag="ACHUOF_002538"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="ACHUOF_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="ACHUOF_002539"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="ACHUOF_002539"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR0611674.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="ACHUOF_002540"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="ACHUOF_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR0611675.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHUOF_002541"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHUOF_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR0611676.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="ACHUOF_002542"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="ACHUOF_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR0611677.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="ACHUOF_002543"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="ACHUOF_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR0611678.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="ACHUOF_002544"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="ACHUOF_002544"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR0611679.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="ACHUOF_002545"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="ACHUOF_002545"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR0611680.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHUOF_002546"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHUOF_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR0611681.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="ACHUOF_002547"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="ACHUOF_002547"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR0611682.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="ACHUOF_002548"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="ACHUOF_002548"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR0611683.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="ACHUOF_002549"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="ACHUOF_002549"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR0611684.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="ACHUOF_002550"
     CDS             10748..11212
                     /locus_tag="ACHUOF_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR0611685.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHUOF_002551"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHUOF_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR0611686.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /gene="selO"
                     /locus_tag="ACHUOF_002552"
     CDS             12236..13672
                     /gene="selO"
                     /locus_tag="ACHUOF_002552"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR0611687.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHUOF_002553"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHUOF_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR0611688.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHUOF_002554"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHUOF_002554"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR0611689.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHUOF_002555"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHUOF_002555"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR0611690.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         4052 a         3759 c         4263 g         4015 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgc
//
LOCUS       DAZOYF010000086        15891 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZOYF010000086 DAZOYF010000000
VERSION     DAZOYF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.086"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..756
                     /locus_tag="ACHUOF_002556"
     CDS             <1..756
                     /locus_tag="ACHUOF_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFR0611691.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="ACHUOF_002557"
     CDS             complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="ACHUOF_002557"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR0611692.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(1792..1995)
                     /locus_tag="ACHUOF_002558"
     CDS             complement(1792..1995)
                     /locus_tag="ACHUOF_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0611693.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="ACHUOF_002559"
     CDS             complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="ACHUOF_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR0611694.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4588..5100)
                     /locus_tag="ACHUOF_002560"
     CDS             complement(4588..5100)
                     /locus_tag="ACHUOF_002560"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR0611695.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            complement(5118..6676)
                     /gene="hpaB"
                     /locus_tag="ACHUOF_002561"
                     /pseudo
     CDS             complement(5118..6676)
                     /gene="hpaB"
                     /locus_tag="ACHUOF_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            complement(6903..7840)
                     /locus_tag="ACHUOF_002562"
                     /pseudo
     CDS             complement(6903..7840)
                     /locus_tag="ACHUOF_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(7867..8757)
                     /gene="hpaA"
                     /locus_tag="ACHUOF_002563"
     CDS             complement(7867..8757)
                     /gene="hpaA"
                     /locus_tag="ACHUOF_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR0611696.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            complement(8767..10122)
                     /gene="hpaX"
                     /locus_tag="ACHUOF_002564"
     CDS             complement(8767..10122)
                     /gene="hpaX"
                     /locus_tag="ACHUOF_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR0611697.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            complement(10144..10947)
                     /gene="hpaI"
                     /locus_tag="ACHUOF_002565"
     CDS             complement(10144..10947)
                     /gene="hpaI"
                     /locus_tag="ACHUOF_002565"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR0611698.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            complement(10958..11761)
                     /gene="hpaH"
                     /locus_tag="ACHUOF_002566"
     CDS             complement(10958..11761)
                     /gene="hpaH"
                     /locus_tag="ACHUOF_002566"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR0611699.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            complement(11829..12209)
                     /locus_tag="ACHUOF_002567"
     CDS             complement(11829..12209)
                     /locus_tag="ACHUOF_002567"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR0611700.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            complement(12219..13070)
                     /gene="hpaD"
                     /locus_tag="ACHUOF_002568"
     CDS             complement(12219..13070)
                     /gene="hpaD"
                     /locus_tag="ACHUOF_002568"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR0611701.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            complement(13072..14538)
                     /gene="hpaE"
                     /locus_tag="ACHUOF_002569"
     CDS             complement(13072..14538)
                     /gene="hpaE"
                     /locus_tag="ACHUOF_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0611702.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            complement(14535..15824)
                     /gene="hpaG"
                     /locus_tag="ACHUOF_002570"
     CDS             complement(14535..15824)
                     /gene="hpaG"
                     /locus_tag="ACHUOF_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR0611703.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
BASE COUNT         3740 a         4148 c         4160 g         3843 t
ORIGIN      
        1 tatactaaga ttttagagaa tgattttgaa cacccttcaa tacgacgaaa aatagcatcg
       61 ctaagtaatt taggggtaga tgagtggatt cctccggtta tggcattcat gaatcgaatg
      121 gctcgtacag aagattttaa tttagatgat ttttctcaat ttattaccgc ctttgagaag
      181 gtatatatgc atgggtggtt aaaaaaacaa attaaaagcc aaagagaaat ggtttgttat
      241 tctgctctgg tagcaataaa taatgatatg ccttttgatt ctgtgataaa tcagataaac
      301 caacatgcag ataattcagg ttttatagct gctctggatg aagatttata tgagcctcgt
      361 ccgaatcaag tgaacttaat aaaagcaatt ttgcttcgct tagatatgga gcaacaagat
      421 gagagcgtga ttaaaaccta tacggggaga atcacaattg agcatattct tcctcaagca
      481 ttagttaatg aatactggat taatagattc caacctcaag agcatgtcta ctggctgcat
      541 aaaataggta atttaacact cataagtggt tccaaaaatt ctgaaacaca acactatgat
      601 tttatcaaaa agaaatctat ttatgagaaa cttaatagta aaagctcttt ttatttaaca
      661 aaagatgttt gtaattcttc agaatgggga ttggctgaac tgaaaatgag acatgaaaaa
      721 atgaaaacgc aattgaagaa gctttggttg gtctgacata tctaaagagt tccactcgta
      781 ataatgttgc ggtggaactc tcaaaaagtt gatctttccc gaacttactt cctcaatccc
      841 gcaaacgccg cccgaatctc ttcctccggc aactgaatcc cgataaacac cattgtgcta
      901 tgcggttttt catcgcccca cggcctgtcc cagtcggcgc tgtagaggcg ctggacgccc
      961 tggaacagca ggcggttagg ttcgccgtca atccacagca tccctttgta acgcagcagt
     1021 ttatccgccg attccagcag caggttttcc atcacgcggg aaacttcgct gatatctacc
     1081 gggtaatcca gttccaccac aattgacgaa atatcgtttt gtttatccgc gataaagtgg
     1141 aaacgcggtt tggtgctgac gacgttctct tccagcataa aaccgttggt gttgaacagc
     1201 aggcccaggt cgatgtcgcc gtgggtgacg gtgtagaccg gtgcgcgggc gttgatgcgc
     1261 gccaggcgtt cacgcagttt ctctgcttcg cctgcgacgt cggttttagt cagcagaata
     1321 cggtcggcgt agcccacctg ggactgggcg atggtgaact ggttcatctg ctcatcggca
     1381 tgtaccgcat ccaccagcgc aatcacgccg tccagcaggt agagctggca taaaatctca
     1441 tgggagaaaa aggtctgaat aatcgggccg ggatcggcca tgccggtgca ttcaatcacc
     1501 agacgatcga actgaatatt gcccttgtcg agattatcca gcaggtcgag tagtgcgtcc
     1561 tccagctcgt tggagcgcga acagcagatg cagccgttgg tcagcgtttt gatctgcgtg
     1621 gcgcggtcgc caatcagttg gtcatcaacg gagacttcgc cgaattcgtt ttcaatcacg
     1681 gcaatcttgt agccgtgttg ttcgttaaga atatggcgca gcagggtagt tttgcccgcg
     1741 cctaaaaaac cggtgagtag ggtaactgca atcgggttca tgctctctcc attaacaaca
     1801 gcgcataccg cctttaccat cgccgccgta gcgcgcgttc tggcgttcgc ggaagaattc
     1861 ttcatagctc atgtacggct tgtcgggatg gttggtcttc atatgctcga cgtagttgtc
     1921 gtagtctgga ataccaatca acatcttcgc cgcctggccg agatattttt ttgcctgtcc
     1981 taagttacca aacatagttc actctgataa gaacaaagcc ccgccgaagc ggggctaaac
     2041 acggttagtg gtgcgaagag atcttcacgc cgccttccgg gattggaacg tacggtgttt
     2101 ctttgtcagt acgtttgtcg ctgttacgca ccgcaagcca ggttttgaaa ccgtagaaga
     2161 tgatgctgta caccacaatc aggaacagaa tactcaggcc tgcgttggtg tagttgttca
     2221 caacgatgtg gttcatgttg gcaatctgct gcgccgtcag gtcagtaccg ttagcaatct
     2281 tctctttgta ctggcttgcc atgtagaaga agccttccat ctgcgggttg gtgctgaaca
     2341 gtttcagacc cagcgcccag gtggtgcaga taagcagcca tacagccgga acaacagtta
     2401 cccagatgta ttgggtgcgc ttcatcttaa tcagcacaac ggtgcccagt accagcgcta
     2461 cggctgccag catctggttg gagataccga acagcggcca caggctctta acgccgccca
     2521 gcggatcgac cacgccctga tacagcaggt agccccacag acccacacag cccgcagtac
     2581 cgatgatacc ggcaaccaga gagtcggttt ttttcaggaa cgggatgaag ttacccagca
     2641 ggtcttgcag cataaagcgg ccagaacggg tacccgcatc cagcgcggtc aggatgaaca
     2701 gggcttcgaa cagaataccg aagtgatacc agaagcccat gtcagccatc ggcagcactt
     2761 tgtggaacac gtgagcgata cctaccgcca gcgtcggcgc accacctgca cggttcagga
     2821 cagaaggctc accaatgtct ttcgcggttt gcaggatctg ctctggcgaa atcacgaagc
     2881 cccaggagct gacggtcgct gccgcgtgtg cggtaacgtc tttcagctgc gccatgatga
     2941 tcggcgcgtt ctcgccaccc atttcatgca ggttaggcat ggtgatgcca aggccagcag
     3001 gcggggtgtt catcgcgaag taaagacccg gttcgatgat ggacgctgca accagcgcca
     3061 taatcgccac gaaggactcc atcagcattg cgccgtagcc aatgaaacgc gcgtcggttt
     3121 cgttagccag cagtttcggc gtcgtaccgg aagagatcag cgcgtggaag ccagataccg
     3181 caccacaggc gatggtgatg aacaggaacg ggaacagagc gcctttccac agcgggccag
     3241 taccgtcaat gtactgggtc atcgcaggca ttttcagttc cgggttcagc accacgatac
     3301 ccagcgccag gccgacgata acgccgattt tcaggaaggt tgccagatag tcgcgcggtg
     3361 cgaggatcag ccacaccggc agcagtgcgg aaacaaacgc atagccaatc agcgcgaagg
     3421 taatggtggt gtctttaaag gtcagtgccg gaccccagta cggatcgtga gcaatcacgc
     3481 caccgaagta gatagaggca accagcagca cgataccaat aacagagact tcacccacac
     3541 gccccggacg gataaagcgc atgtagatac ccataaacag cgcaatcggt acggttgagc
     3601 aaacggtgaa gacaccccac ggactttcgg ccagggcttt aaccacaatc agcgccagaa
     3661 cggcgaggat gatgatcatg attaagaaac agccaaacag cgcgatagtc cccggtactg
     3721 gccccatctc ttctttgatc atctcaccga gagatgcgcc gttacggcga gaggagataa
     3781 acagcaccat aaagtcctgt accgcaccgg ccagcactac ccccgccagc agccacagcg
     3841 tgccaggcag gtagcccatc tgcgcggcga gaaccggtcc caccagcgga ccagcaccgg
     3901 cgatagcggc gaagtggtga ccaaacaaca cgtaacggtt ggtcggaacg tagttcagac
     3961 cgtcgttgtt aataaccgca ggcgtcgcgc gcgtggggtc gagtttcatc accttctggg
     4021 cgatgtacag actgtagtag cgatacgcca ccagatacac cgacacagag gcgaccacga
     4081 tccacagggc gctgacgtgc tccccccgac gtaatgcaac taccgcgagg cagaatgcac
     4141 cgatgattcc gagaatcacc cagggtatgt gcttgaatag ttttttcgta tccatagtaa
     4201 aacctggcat tttactcgtc gtcttttggc ttacaggtgc ggcagctgcg ttcgttcacc
     4261 tctgacactt acttttgtaa gctcccgggg attcactcac ttgctgcctt cctgcaaacc
     4321 aaaatccata gagtaatttg ttaatcatag gccgaagccg ttctaggttt gcgttgcgtt
     4381 tgaggaggta aattgaccgc tatcctgcag tgatgttgcc agagatacgt gcgcgtaaag
     4441 taaggtaaat aactgagtgg ttattttagg gatgtaagcg gtcaggaatg gcggtgagcg
     4501 gttgggcaag aattattcat cgggtagata tgtgattgag atcacaataa aaatgcccct
     4561 tcgacgaatg cgtgaagggg ctggagctta aatcgcagct tccatttcca gcatcaccgg
     4621 atggaaacgg cgtttaaagt aaataagtcc gtgaccttct gcactgagga tgatgttttt
     4681 aatctccacc agatacacca gatgtgtgcc aatcgcctgc acatcgcgga tctctccttc
     4741 aagactggcc agcgaacctt ttagcaccgg ctgcgccagc ggaccttttt gccagcatga
     4801 gaggctaaaa cgctcttcca tcgccatgcc tgtcatgccc gcgaagtggc gcgccatcag
     4861 ttcctgctca tggttgagga cgttgacgca caacttgccg ttgccctgaa aaaccgggtt
     4921 catcgcactg ttggcgttaa tgcacaccat cagcgacggt ggtgtatccg tgaccgagca
     4981 gacagccgtt gccgtaatcc cgcattgtcc ggcgtcgccc tcggtggtga taatatttac
     5041 cgctgccgac aggctggcca tcgcgtcatg aaagcgcagg cgttgttcat ctaattgcat
     5101 cttaacctcc tgctgcgtta tttcagcagc ttatccagca tgttgatatc gtcgttgttg
     5161 tgcaggtgcg gcacagtcca gccgttctgg tcgtattccg acaggcagcg atcaaccatc
     5221 gccatcatct tgtccatatt gccggagctt tgtgcctggc gcagacattg caggcgaatc
     5281 tcatcctggc taccggagta gttgatttca tacagctcgt gacgaccacc aaattcgctg
     5341 ccaatggcgt cccacatcag tttgaggatc ttgatgcgct ggacgtgatc cataccgttc
     5401 gaaccgcgca catacttcgc cagatactgg tcgatctgcg gattgttcag gtcacgggca
     5461 ctggaaggga ggtagatcag gccactggta acgttgcgtt ctataatgtt tttgatcttc
     5521 gcgtaggcca ttggtgccag tacgcgatag gtttgcagtg cggcatgatc cggtaaataa
     5581 gccccgttga cccacggcgt cgcttctgaa cacatcgagt cactcaatgc ccagaaggtg
     5641 ttgcgccacg ccaccacttc accgagatcg gcctgcacac cacggaactc cagggtgccg
     5701 gtacattcga gtgatttttt cagcagtgcc gtaatgaagt cgagtttcac tgccaggcgc
     5761 acacaggctt gcagcggata catacgggcg aaaccgcctt ccatcgtcca gcgacggcag
     5821 cgatcaaaat cgcggtagat cagcacgttt tcccatggga tcagtacgtt atccatcacc
     5881 agaatcgcat cgttctcatc gaagcggctg gagagcgggt agtcgtatgg cgagccggta
     5941 gcacccgcga ccatctcata agaggcgcgg gagattaatt tcacgccatc ggcatccatt
     6001 ggcgcaacga acatcagtgc gaagtccggg ttttcgccca tcacttgtgc cgagccgaag
     6061 ccaatcatgt tgtagtgagt cagcgccgag ttggtggcaa ccactttcgc accgctgacg
     6121 ataatcccgg cgtcagtctc tttttccagc ttgatgtaaa cgtctttcac tttatcggtc
     6181 ggcaaatgac gatcggtggg ttaacaatcg cgtggttaaa gtagaggcca gtttcctgaa
     6241 tacgggtata ccagttacgg gcgttctgct cgaactgacc gtaaaagccc ggattcgcgc
     6301 ccagtgcgca accgaaagcg gctttgtagt ctggggtacg gcccatccag ccatagctca
     6361 ggcgtgacca ttcagcgatg gcgtcgcgtt gctggcgcag gtcgtcggca cttttcgcca
     6421 cgcggaagaa tttatgggta tagccgccgc tgccggtgtc ggtgttccaa cacagagagt
     6481 cctgcatctc cggtttgtgc agcgcgtcgt acagttgggc aacagacgct gccgcattac
     6541 gaaatgccgg atgagtggtg acgtctttca ctcgctcgcc atagatatag atctcgcgac
     6601 catcctgcag gcttttcaga tactcttccc cggtgaaagg acgttgggta ctggcgcgga
     6661 aatcttctgg tttcatgtcg acctctacag tattaatcgg aatacgattg ttaattttgt
     6721 gtgttgtttt tgttatttaa ttgaagcgtt gaacgcggtt gtcgggaaga gacgtttcag
     6781 gcgaacgttg gtacttttcg acagcaaggt ggagttaaat agtaaactgc gatcgtgatc
     6841 aacttacagt aaggcaaatc ataatattcc ccgctaacaa gggctaacgg ggacggaatt
     6901 ttttatgggg cgaatggaat ggtaatcgtc atattttcat gaccaacgac gataggacct
     6961 gaatagtttt ctgtgcttct tgcttccatg tttcatccgg aacggtgatt ggtaccaggt
     7021 ggctaaggat gagttttttc acttttgctt cattggcaat tttgcctaca tcctcaatag
     7081 tcgtatggtg agaagcaatg gcttttgcca gtgttttacc attgccaata ctgtctgcca
     7141 gcttagcgac ggagggctca tgtacggcct catgcacaag tatatcagcg ccttctgaga
     7201 aagttgccag tgggggaaag tacgaggtat ctcctgagaa gacaattcgc ttgccttgtg
     7261 tgtcgaagcg ataagcaaaa gcctcaccgt cagggaaagg agaatgaggg acctttaatg
     7321 cagtcacggt cacggtatca tctttataaa ctacgccttc accaacatct tgagctgtaa
     7381 cgagtttgcg aggatcgggt tttccttctt cttccatacg cagtgtgatg tcccgatcga
     7441 agacctgcca actggcagcg gtcatggctc gggtgcctgg cggaccgaat acatgaatag
     7501 ttttgtcctg cagaccgcta gcccatgcat tcattaagac tgatggatag tccagaatat
     7561 gatcactgtg taaatgggtg agtaaaatat tatcaatctg gcgcagtgga atattatttt
     7621 ttaccagttg taagctggcc ccgtaaccag catcaattaa ccaggtttta ttgccaataa
     7681 taagtgccgt tgccatcggt aaacggtcag cttttaataa agaagggcca cctttcgttc
     7741 caagtagtac aattttcatt tattgcggtt cggaggcgta ggtgaatgcc actggcgcaa
     7801 tcaggcacgc cacaaatagt gagcgtaaag atattttcat gtcgagtctc tcaggttgat
     7861 aaatggtcag cgaccacgga attgtgttgg tgagcaaccc atcattcggt tgaaaaaacg
     7921 ggcgaaatag gcgggatctt tgtagccaag atcaagggct atctggttga tgctttgctc
     7981 gctatagagc agaagccgtc tggcttcccg gctcactcgt tcgaaaatca atctcttcgg
     8041 cgattgattg gcaaaacgtc ggcacagctc ggtaagccgt gattcactca taccgagcat
     8101 tgttgcatat tcagggacca ttaaatgttg gcgataattt tcgtcaatca gccgattaaa
     8161 cttctggaat aaacacatct cgccgcgcac gctgcatgtt gaaatatcat ttggtggtgc
     8221 ttcacgcagt aggattgtaa aaatactttg cgccagtgat tgcaatagaa tttcccgtcc
     8281 ttgcttattt tgttgaaact ccagggatat tatcggccaa taatgattta atgccgccag
     8341 tgtatgttcc attccctcca gtgacagaca gataccttgt agcattgcgg catctccact
     8401 accaggccag agcttttcta ccaggggcca aattaactcc tgacgtacgg tcagaacatg
     8461 cccatcgcta tcaggatcag taaagaaagt atgtggcacc gatggtggtg ttagtacaaa
     8521 aagcggtgcg cgcacagaat agaagtgctc atccagttgt aaagtaatgc gtccagtgac
     8581 cagataatgg agttgaaaat atctatcgtg ccagtgaggg cgcatatcgc gtccaaagaa
     8641 attcgctaac cgggcaaatg tttcataatg cacatcttca ccagcgtagc gggcatcata
     8701 ttcccggacc aaatcaatat tttcataacc atgttcaaag atagcttcac tggacatcat
     8761 gactccttat tgcggttttg gtgcgataag ttcagatgaa agagagtgtt cccgactccc
     8821 catagggatt cgggtcatga ctaacgcgcc cagtaccagc aatcctgcta caaaccacaa
     8881 acctgagcta aaactgccgg ttgcatcttt caaaataccg ataagtaatg gactgactgc
     8941 tgaacctaca ttgccgacag cattgatgac agccatcgca acggcacgcg attgcaagct
     9001 tataacttga tccggtgttg tccaaaagat agtcatcgcg gtaaatgatc ccattgaggc
     9061 cataatgatg ctgctcagtt gaatgaggct gtgatctgtc gcagaagcga gtaaccatcc
     9121 ggcagcggca aacaggtagg gaagaatcgt atgctttttg cgttctttaa gacggtcgga
     9181 acgacgactc caccagatca tgccaaaaat tgtgcagaat tgggggatgg ccgccagcaa
     9241 accaatcatg atgttgctac tgccagtatt gaaactctgt aggatttgcg gcgtccagat
     9301 attaatcgca ctcagagtat tagtcgggca aaaataagca agtgtatata gcatgatggc
     9361 tggggtcagg acttcacgta aggttgagcg tggaacagat gcatgggcaa atgcactctc
     9421 ttgttcacgg agaatcatcg tttttaaagc ctgtttttcg ttttcatcaa gccaggtcgc
     9481 ctggtctggt gtgtcgttga gataaaacca ggtgacgaca ccaagaatga ccgatggcag
     9541 accttccagc aaaaagagcc attgccagcc ttttagattc cacagtccat ccaaggccag
     9601 aatgtagcct gaaaggatca atcccagcat catagtgact ggcatcgcta tcataaataa
     9661 ggcattggct ctggcccgat gatatgccgg gaaccaccag gtcagataaa ccaaaatacc
     9721 aggtaaaaaa cccgcctcag caataccaac cagcatacgc agtagataga gtgtttcggg
     9781 gcttgttgca aacattgtgc aggtggaagc aattccccag accaccatga tcccagcgat
     9841 ccatcgacga gcgccaattt tcgctaacat catattactg gggatcccgc acatgacgta
     9901 cgtgacataa aagagtgttg cggcgaggcc aaacattgtg gacgtcaggc caagatcctt
     9961 gcccattgtc agtccggcaa atcctatatt gatgcgatca agaaacgaaa aaacaaacag
    10021 aataaaaaga aaaatgatta ggcgacgaaa taatttatta atgacacgat gttcaatttc
    10081 aacagtttta ttatctttat attgcagggt agaggtcgtc atgatgcgct ccacatcgta
    10141 cgtttagtag acagagttgt tgttttgctt cgccatcgaa atagaagaac tattaccaaa
    10201 ccgtgctgcc agcgcttcgg cggcgcgggc gagcagggtg gtgtcaacgc cgacggcgac
    10261 aaacagcgcg cccagttcca gatagcgttt tgccagttgc tcattggcga tcaggatccc
    10321 cggcgctttg cccgcttcgc ggatctgcac gatcgcctgc tcaatggcgg cctgtacttc
    10381 cgggtgctgc ggattaccgg cataacccat atcggcgctc agatccgccg ggccgataaa
    10441 cacgccgtcg accccttcca cgtccagaat ctgcggtaag ttcttcattg cctcacgcgt
    10501 ttcgatctgc accagcacgc acatttgatc gttggctttt tgcaggtaat caggaatgcg
    10561 attccagcgc gaggcgcgag ccagcgcact gcccacaccg cgaataccgg cggggggata
    10621 acgggtggcg cgtaccgctt tacgggcttc gtcggcgttt tgtaccatcg gcaccagtaa
    10681 ggtttgtgtg cctacgtcca gcagttgttt gatttgcacc ggatcgttcc acgatgggcg
    10741 taccactggc tggctggggt agggcgcaat cgcctgtagt tgggtgagca cggtttgcac
    10801 gttattcggc gcgtgctcac cgtcgatcaa taaccagtcg aatcctggtc cggccagtaa
    10861 ctccgcgctg tagctgctgc tcagccccag ccataatcca atctgcggac ggcctgcttt
    10921 cagcgccgct ttaaaactgt tttccatcgt tctctcctta aacaaagcgg cagctaatgg
    10981 agcccatgtt gccgtaatcg acgtggaagg tgtcgccctt acgcgccgga accgggaggg
    11041 tgaacgaacc accgagaatg atttgcccgg tttccagttg tacgtcatac ggtgcgagtt
    11101 tgttcgccag ccaggccacg ccgtttgccg gatgattcag cacaccagcg gcgacgccgg
    11161 tttcttcaat cacgccattg cgatacatca gggcggagat ccaacgcaga tccagttcgt
    11221 ctggtttgat cggacgacct ccgaggatca ctccggcgtt ggcggcgtta tcggaaatgg
    11281 tgtcgaatac tttgcgcggg cgctgggttt ccggatcgat gttgtggcag cgagcgtcga
    11341 tcagctccag cgccgggatc acatagtccg tggcgttgta aacgtcgaac agcgtgcagt
    11401 ttggcccacg cagcggtttt gccagcacaa aagccagctc cacttcaatg cgcggcacga
    11461 taaagcgatc ggtcgggata tcgctgccat cgtggaagaa catgtcgtcc agcagtgcac
    11521 cgtaatccgg ttcgctgatc tgcgagcttg cctgcatcgc tttcgaggtc agaccgattt
    11581 tgtggccttt cagcgtgcga ctttcggcga ttttcaggcg aacccattcg cgctgcacgg
    11641 cgtaggcgtc ttcgatggtg atctccgggt aatccagcga gatcgcgcgg atctgttcgc
    11701 gctgtttttc tgcctgatcc agacgctggg cgatcagggt gtgggtgtgt ttatcgaaca
    11761 tagcgatatc ctgtggcatt gccggatgcg gcagcgcctt atcatccggc ctggaagctg
    11821 gtgcaaattt atttaaacaa cgcgtgtacg ttgttctgtt tgaaattcaa cgtcgggtgc
    11881 agctctgcaa tctcaaatga aagcgctagg taacggctct ccatcagtga ggcgaagtgc
    11941 gttttaatca gatcaaacag catttcgccc acttcctgac ggctttccag actgcgtccg
    12001 ctgccgattt ttagcgtcat atgcacaaag gcgtaatcgt gcttgccatc agccatttgc
    12061 caggtatcga tccagtgcgc ccggctacgt attcccccaa gcggaaaaat ccccgttgcc
    12121 gccagtgcag tgttaacact ggcaaacagc tcaggtaagc gggcttcttc acgaatattt
    12181 tcagtacatt ccacgataaa gtgcggcata aaacgtcctt acgcgggcag cgggaaaaca
    12241 gcgttaacct gaccggtgcc agagcttggg aacagctcgg taataaactc caccttgccg
    12301 tcgtatttat cccagccgag catccccagc agcatcaccg tgtcgtgcat attgccttcg
    12361 ccgtagcagt agtcggcgta ctccggcagc atattgcaaa actctttgaa ctggccttcg
    12421 cgccacagct tcaccacacg ctcgtccatc tggcggtcga actcgcgggt gtagctgttc
    12481 atcccttctt ccgcacgctg atcgtcaata aagcggtgcg ataacgaacc gctggcaagg
    12541 accgccacgg tgccgtcgta ctgttcgatc gctttcagaa tcgcttcgcc cagcttgcgg
    12601 ctgtcggcaa aatcgtgaac cgtgcagaaa gccgaaatgg agaccacttt gaagtgcttg
    12661 tcttcattca tgtagcgcat cggtaccagc gtgccgtatt ccagtttcag gctgggaata
    12721 ttgtgcgctt ttgcccgcac gccgagcttt aaggcttcct cggcaattag ctgccccaac
    12781 tccgggttgc cctcgtagtt gtaggtcatg tcgcgaataa aatgcggcag ctcgttgctg
    12841 gtgtagacgc cttcaaaatg gtctgcacag ttgatgtgat aagcactgtt gaccagccag
    12901 tgagtatcga aaacgataat ggtatcgacg cccatttccc ggcaacgctt gctgatctct
    12961 ttatgcccgt cgatcgcacc ctggcgacaa ccgtggtttt ttcctggtag ttcagagaga
    13021 tacatcgacg gtacgtgcgt gatcttggcg gctaacgcta acttacccat atcagactcc
    13081 ccatttcgga attggatggt cgcccatgga aatgcagacg ttcttcattt ccgcgaacac
    13141 ttcgaagctg tactcaccgc cttcacgccc ggtgccggag gcttttacgc cgccaaatgg
    13201 ctggcgcagg tcgcgcacgt tctgggtgtt gacgaacacc atgcctgctt caatgccacg
    13261 cgccaggcgt aacactttgc tgacatcctg tgtccagatg tacgacgcga ggccgtactc
    13321 cacgtcgttt gccaggcgta agccttcggc ttcgtcttta aacggcagca ggcaggcgac
    13381 cggcccgaaa atctcttcct gggcaacgcg catacggtta tctacgtccg ccagcaccgt
    13441 tgggcgcagg aagttgccgc ctttcaggtg cgcaggcagg tcggacggtt tatccgggcc
    13501 gcccgccaac agggttgcgc cttcttcaat gccgagacgg atatagccgg agactttttc
    13561 ccagtgttgc tgactgataa gcgccctaac ctgggtattc ggatcgttcg gatcgcccac
    13621 gcgcagacgg ttggcgcgtt cggcaaagcg tttaacgaat tctggataga tgctctgctg
    13681 aataaagatg cgcgaaccgg cggtgcagcg ttcgccgttg attgagaaga tggtgaacag
    13741 ggcggcgtcc agcgcacgct caatatcggc atcttcaaaa atcagcaccg gtgatttacc
    13801 gcccagctcc atggagtatt ttttcagccc ggcgtttttc atgatgttgc gcccggtggc
    13861 ggtaccgccg gtgaacgaca cggcacgcac gtcatgatga cggaccagcg catcgcctgc
    13921 ggttgcgccg tagccctgta ccacgtttag cacgcccgcc ggaataccag cttccagcgc
    13981 cagctcaccc aggcggtcgg cggtcagcgg ggagagttcc gacatcttca gcaccgcggt
    14041 attgcccagc gccagacacg gcgcgacctt ccaggtggcg gtcataaacg gcacgttcca
    14101 cggcgacgcc agcgcacaaa cgcctaccgg ttgcaccagc gtgtagttga gcatcttgtc
    14161 gtcaaccgga taggtcttgc cgttcatctg ctggcagact tccgcgaaaa attcaaagtt
    14221 gtgagaagcg cgtgggatca acacattttt ggtctgatgg atcggcaggc ccgtgtccgc
    14281 ggtttccatc gcggcgatct caggcacgtt ctggtcgatc agatcgccca gacggcgcat
    14341 caggcgcgca cgctctttca tcggcagatc agcccatttc gggaacgcct ctttcgctgc
    14401 cgctaccgcc tgattgatct ccgcttcacc gccagaggcc acatccgcca gcacttcacc
    14461 cgttgccgga ttggtagtct ggaagtagtc gttacctgca acatttttgc cgttgatcca
    14521 atgatttact tttttcattt cgctgtttcc tcactcacaa ttcggttcac caggcggccc
    14581 acgccttcta cttccaccac tacttcatcg ccaggcacca cgtcagataa gccttttggt
    14641 gtgccggtgg cgatcatgtc gcccggattc agggtcataa attcgcttaa gtaggcgatc
    14701 aggaagggca cgctgaagat cagatcggcg gtggtgcctt gctggcgtaa ctcgccgttg
    14761 acgaaggtgc gaagggtcag attatgcggg tccgggatcg cctctttcgg cacgatggtt
    14821 gaaagcatcg gcgtcagtcc gtcgcggctt ttgacccgca ggttagggcg gtagtagttt
    14881 tccagatagt cgcgaatggc gtagtcgtta cacacggtgt agcccgcgac ataatccatg
    14941 gcatcggctt cgctgacgtt acgcgcctgc ttgccaataa ctaccaccag ctccgcttcg
    15001 tagtgcatgt attcaatatt gttcgggcgc acggaggtct ggttgtcgcc agtgagggta
    15061 ttcggcgctt tcaggaacac cagcggctct tccggtggct taaattccag ttcgctggcg
    15121 tggtcggcgt agttcaggcc gagggcaaac agcgtaccgt gcgggtgtgg cagcgttggg
    15181 aagctcttgc gcgtggtcac ttcacgttcg tccactaccg gattttccag cggcgggaaa
    15241 ccttctgcga gaacacgcac gcgatcgctt ggctgtattt ccacgcgcgc ctgtggcgtg
    15301 ccgagcagaa tagcatcgcc tggattcagc gtggcaaatt cgctcagggc gctcagcagc
    15361 tgtgcggcgt tacgttgtaa atcggcggta ttccagtgat cggcaggacg cccgttgatc
    15421 tcggtataga tagtcagatt atcgacattg ctgagagcca cggtttcgcc aatggggcag
    15481 aatccatcac gacattttgc tttgattgcc ggacggtaaa agctctcttc cggcaggctg
    15541 acgtcgttat ccagcgcata tccggcgatg tactctgccg catcttcttc acgtactttc
    15601 gtcgccgttt tccccacaat cagcgcaaca gtcgcaccgc tcagtacttt ttcaccctgt
    15661 ggaaagggaa tcggttcacc gcaaccaatc accgtattgc gcggtttaat aaaccagacc
    15721 gcagttttag gcggggcttt gtagggagat tgctggaacg cttcctgcca ggcatcaagt
    15781 tggctgcgat ggttcaacgc tacggcgaag atagtgcctt tcattaccac tcctcggatt
    15841 cgatcatctt aatcattaat atgttaatga ttacttttat tcccgattgc g
//
LOCUS       DAZOYF010000087        15526 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZOYF010000087 DAZOYF010000000
VERSION     DAZOYF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.087"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACHUOF_002571"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACHUOF_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFR0611704.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACHUOF_002572"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACHUOF_002572"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFR0611705.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="ACHUOF_002573"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="ACHUOF_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHUOF_002574"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHUOF_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR0611706.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="ACHUOF_002575"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="ACHUOF_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR0611707.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="ACHUOF_002576"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="ACHUOF_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR0611708.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="ACHUOF_002577"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="ACHUOF_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR0611709.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="ACHUOF_002578"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="ACHUOF_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR0611710.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHUOF_002579"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHUOF_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR0611711.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="ACHUOF_002580"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="ACHUOF_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR0611712.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHUOF_002581"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHUOF_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR0611713.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="ACHUOF_002582"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="ACHUOF_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR0611714.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHUOF_002583"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHUOF_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR0611715.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHUOF_002584"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHUOF_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR0611716.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="ACHUOF_002585"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="ACHUOF_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR0611717.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="ACHUOF_002586"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="ACHUOF_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR0611718.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="ACHUOF_002587"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="ACHUOF_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR0611719.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHUOF_002588"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHUOF_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR0611720.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHUOF_002589"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHUOF_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR0611721.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="ACHUOF_002590"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="ACHUOF_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR0611722.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="ACHUOF_002591"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="ACHUOF_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR0611723.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHUOF_002592"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHUOF_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR0611724.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHUOF_002593"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHUOF_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR0611725.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="ACHUOF_002594"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="ACHUOF_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR0611726.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="ACHUOF_002595"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="ACHUOF_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR0611727.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHUOF_002596"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHUOF_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR0611728.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHUOF_002597"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHUOF_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR0611729.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHUOF_002598"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHUOF_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR0611730.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHUOF_002599"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHUOF_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR0611731.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHUOF_002600"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHUOF_002600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR0611732.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHUOF_002601"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHUOF_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR0611733.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4007 a         3642 c         4130 g         3747 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ttggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca atgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctgtac tctttt
//
LOCUS       DAZOYF010000088        15525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZOYF010000088 DAZOYF010000000
VERSION     DAZOYF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.088"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..75)
                     /locus_tag="ACHUOF_002602"
     CDS             complement(<1..75)
                     /locus_tag="ACHUOF_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HFR0611734.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(173..1282)
                     /gene="anmK"
                     /locus_tag="ACHUOF_002603"
     CDS             complement(173..1282)
                     /gene="anmK"
                     /locus_tag="ACHUOF_002603"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR0611735.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1556..2023
                     /gene="slyB"
                     /locus_tag="ACHUOF_002604"
     CDS             1556..2023
                     /gene="slyB"
                     /locus_tag="ACHUOF_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR0611736.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2070..2504)
                     /gene="slyA"
                     /locus_tag="ACHUOF_002605"
     CDS             complement(2070..2504)
                     /gene="slyA"
                     /locus_tag="ACHUOF_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR0611737.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2705..2941
                     /locus_tag="ACHUOF_002606"
     CDS             2705..2941
                     /locus_tag="ACHUOF_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR0611738.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2944..3801
                     /locus_tag="ACHUOF_002607"
     CDS             2944..3801
                     /locus_tag="ACHUOF_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR0611739.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3801..5813
                     /locus_tag="ACHUOF_002608"
                     /pseudo
     CDS             3801..5813
                     /locus_tag="ACHUOF_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5814..6335)
                     /gene="sodC"
                     /locus_tag="ACHUOF_002609"
     CDS             complement(5814..6335)
                     /gene="sodC"
                     /locus_tag="ACHUOF_002609"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR0611740.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6416..7312)
                     /locus_tag="ACHUOF_002610"
     CDS             complement(6416..7312)
                     /locus_tag="ACHUOF_002610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR0611741.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7361..7600)
                     /locus_tag="ACHUOF_002611"
     CDS             complement(7361..7600)
                     /locus_tag="ACHUOF_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR0611742.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7703..8302
                     /gene="nemR"
                     /locus_tag="ACHUOF_002612"
     CDS             7703..8302
                     /gene="nemR"
                     /locus_tag="ACHUOF_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR0611743.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8339..9436
                     /gene="nemA"
                     /locus_tag="ACHUOF_002613"
     CDS             8339..9436
                     /gene="nemA"
                     /locus_tag="ACHUOF_002613"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR0611744.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9517..9924
                     /gene="gloA"
                     /locus_tag="ACHUOF_002614"
     CDS             9517..9924
                     /gene="gloA"
                     /locus_tag="ACHUOF_002614"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR0611745.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10027..10674
                     /gene="rnt"
                     /locus_tag="ACHUOF_002615"
     CDS             10027..10674
                     /gene="rnt"
                     /locus_tag="ACHUOF_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR0611746.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10767..15225
                     /locus_tag="ACHUOF_002616"
                     /pseudo
     CDS             10767..15225
                     /locus_tag="ACHUOF_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15269..>15525)
                     /locus_tag="ACHUOF_002617"
     CDS             complement(15269..>15525)
                     /locus_tag="ACHUOF_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR0611747.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3561 a         4109 c         4079 g         3776 t
ORIGIN      
        1 aaccagttga ttaaaggcgc tacaaccgga gagaaggacc ggcaaaatga taattaacag
       61 ttttttcata gtcatatccc gaagactttc ctggtccgga gggcaaatac cctccttaat
      121 cttccaagtg taacggcaga cacgataaga aaaaattcag ttaattctga tatcacgagt
      181 tggcggggaa aatagccccc agtaccgtct cctggcttgc gccagtgacg gaaggcagat
      241 ttcctggtaa tcccgccagc gtccgccagg caagccaggc gaaagccaat gcttccatgt
      301 catcgccact aatgccaacg gcatcggtgg tggtgacttc tgtgcctagc agtaataccg
      361 ccagacgcgc catgagtagc ggattacgac tacctccacc acataccatc aatcgttcgc
      421 agccaccgct caacaaaact tgttcagaaa tggtcacggc ggtgagttct gccagtgtgg
      481 cctgaacatc acggggatca acacccggaa aatggcgtaa atggcgctcc agccagccgt
      541 agttaaagta ttcgcgtccg gtgcttttcg gtgcaggttg cgagaaatac gggtcgctga
      601 gcatattttg cagcagtggg agaataactt tacccgcccg tgcccactcg gcatctttat
      661 cgtaaggttt acctgcctga cgccagatcc aggcatccat cagcatgtta ccaggaccgg
      721 tatcgtagcc gccaaccagc tgcccaggaa tgagcagtga cagattggcg atgccgccaa
      781 tattgagcac cattcgtcgc tcggttgggt gagccagcaa ggcatgatgg aacgcaggta
      841 ccagcggtgc gccttgtcct cccaaggcaa tatcgcgacg gcgaaaatca ccgacaaccg
      901 taattccggt gcgtgccaca atttgattgt tatcgccaat ctgtaaagtg tgtggtgcca
      961 cgcccgtcgg ttcatgccag acggtttgac cgtgacagcc gatcgcaact atatctctcg
     1021 cctgcaggtt ttgttcctta agcaaggcat tgaccgcatc agcaaaaagt cgcccgagtt
     1081 gagtatcaag ctgtccaaac tgcgaaagtg taagctgctg gccctggcaa atatccagta
     1141 cagcctgttt cagagacacc gggatcggcc agctcaagct tgccagctgt gcgacccggt
     1201 gttcatcaat tgtcgccaac acaacatcaa caccatcaag gctggtgcct gacataacgc
     1261 caataaagcg gcccgatttc atagttcatc ctttttcaat ctgacgtttg cgcaccactc
     1321 aaacataaac ttttcgtgaa taccatgcgg aatgaccgat ttttaccgtt ggtagtaaaa
     1381 cattatcttc aaatcaataa tcatcatgaa tgttttgttt ataattggtt gatcctactt
     1441 tcattatgat ttgctcatat ttggtagaac atgtaaccat ggattcacat atgccatata
     1501 ctttgaccat gagggatgct tgcgtggcgt ttcatggtga acaggagatt tttcaatgat
     1561 taaacgcgta ttggttgttt caatggtagg tctgtctctt gtcggttgtg ttaataacga
     1621 caccctgtca ggggatgttt ataccgcttc tgaagcgaaa caagtacaga atgtcagcta
     1681 tggcaccatc gttaacgtac gtccggtaca gattcagggc ggtgatgatt ccaacgttat
     1741 cggtgcaatt ggcggtgctg ttcttggtgg tttcctgggg aatactgttg gtggcggaac
     1801 cgggcgttct ctggctactg cagcaggcgc tgttgcaggt ggcgtagctg gtcagggcgt
     1861 acagagtgca atgaacaaaa cgcagggtgt cgagctggaa attcgtaaag acgatggtaa
     1921 taccatcatg gtggtacaga aacaaggcaa cactcgtttc tctccgggcc aacgtgtcgt
     1981 actggccagc aatggcagtc aggtgaccgt ttctccgcgc taaataagtt tgcgtgtggt
     2041 caggttactg accacacgcc cccttcattt caccctttgg cctgtaactc aatgatatta
     2101 tgctcaagtt ttgcgatgag cgtaatcagt tgctccagtt cctctgcgga gatgccatgt
     2161 aatatttccg cgcgggtttt gttaataaca gcttccattt cgctgatcag tggctctgcc
     2221 ttttccgtca gtttaatacg tttagcccga cgatcgctgg cacaagtttg acgcgaaatt
     2281 aacccttttt cttccagttg gtccagagta cggaccagtg atggctgctc gatgccaatc
     2341 gcttttgcca gttgaatttg cgactggtct ggaggtaact gatggatatt gtgtaacgta
     2401 acccaatggg tttgtgttaa ctccagcggt ttcaggcgat ggtctatcag agcacgccat
     2461 atgcgcacca accgtgccag atcagaacct agtggcgatt ccaatttcat ctccttataa
     2521 ttagcttgct aagatattat gcggctttta gaatagtgtg cagcaattgt attgctaaaa
     2581 caaatgtatt gctgcatttg gttaccgtca gacatatttt tcagaaattg cgcgtaaatt
     2641 tttcgcactt aaagaatatt tattaatcta acgcaatata ttcggtcgta aaggaatcta
     2701 ctttgtgaag tttatgctca atgcaacagg attgccctta caagatctgg tgttcggtgc
     2761 gtccgtctat tttcctccgt ttttcaaagc attcgcgttt ggattcgtca tctggcttgt
     2821 cgtacaccgc ctgcttcgtg gctggatcta cgccggcgat atctggcatc ccttgttaat
     2881 ggatttatcg ctgtttgcga tttgcgtttg ccttgctctg gcaatactga ttgcgtggta
     2941 actatgtcaa ttaaaacaat taagtatttc tcaacaatca ttgtagcgat agttgcggtt
     3001 cttgccggat ggtggctgtg gaattattac atgcaatctc cgtggacacg cgacgggaaa
     3061 atacgcgcag aacaggtttc tatcacccca caggtgtcgg gacgtattgt tgagctgaat
     3121 ataaaagaca accaactggt taacgcgggg gaacttctgc tcacgatcga taaaacgccc
     3181 tttcagatcg ccgagctgaa cgcacaggct cagctggcga aagcgcaatc tgacctcgca
     3241 aaagccaata acgaagcgaa tcgtcgtcgt catctctcac aaaattttat ttttgccgaa
     3301 gaactcgata ccgctaacct caatgttaaa gcgatgcagg ccagtgttaa tgccgcacag
     3361 gcgacgctga aacagacaca atggcaactg gcgcaaacgg aaattcgcgc cccggtgagt
     3421 ggatgggtga ctaacctcac tacccgcatc ggcgattacg ctgacaccgg gaaaccgctg
     3481 tttgcccttg tcgacagcca ctcgttttac gtcattggtt attttgaaga aaccaagttg
     3541 cgccatatcc gcgaaggtgc accagcacaa attaccctct atagtgataa caaaacgtta
     3601 cagggtcacg tttccagtat cggtcgggcg atttatgatc agagcgttga aagtgactca
     3661 agccttattc cggatgtgaa acctaacgtc ccctgggttc gtctcgccca actcgttccc
     3721 gtccgctttg cgctggataa agttccaggc gatgtcacgc tggtgtcggg caccacctgt
     3781 agcattgccg taggtcaata atgaacgcat cgtcatggtc cttgcgcaat ttgccctggt
     3841 tcagggccac gctggcgcaa tggcgttatg cgttacgcaa taccattgcc atgtgtctgg
     3901 cgctgacggt tgcctattat ttgaatctgg atgaacccta ttgggcgatg acctcggctg
     3961 cagtggttag ctttcccacc gttggcggtg tcatcagcaa aagcctcgga cgcatggctg
     4021 gcagtttact gggagccatt gcggcactgc ttcttgccgg acatacgctc aatgagccgt
     4081 ggttttttct attgagcatg tcggcgtggc ttggcttttg tacctaggcc tgtgcgcact
     4141 tcacgaataa cgtcgcgtat gcatttcaac tggcgggcta cacggctgcc atcatcgcct
     4201 ttccgatggt taatattact gaggccagcc agctgtggga tatcgctcag gcgcgcgttt
     4261 gcgaggtgat agtcggtatt ttgtgcggcg gcatgatgat gatgatcctg cctagcagtt
     4321 ccgatgctac tgccctttta actgcattga aaaacatgca cgcccgatta ctggaacatg
     4381 ccagtttact ctggcagcct gaaacaaacg atgccattcg tgcagcacat gagggggtga
     4441 ttgggcagat actgaccatg aatttgctgc gtatccaggc tttctggagc cactatcgtt
     4501 ttcgccagca aaacgcgcgc cttaatgcgc tactccacca gcaattacgt atgaccagtg
     4561 tcatctccag cctgcgacgt atgttgctca actggccctc accgccaggt gccacgcgag
     4621 aaattctcga acaattgctg acggcgctcg ccagttcgca aacagatgct tacaccgtcg
     4681 cacgtattat cgccccgcta cgcccgacca acgtcgccga ctaccggcac gtcgccttct
     4741 ggcagcgact acgttatttt tgccgccttt atctgcaaag tagtcaggaa ttacatcgtc
     4801 tgcaaagcgg tgtagatgat catgccagac tcgcacggac atccgctctg gctcgtcata
     4861 ccgacaacgc cgaagctatg tggagcgggc tgcgtacatt ttgtacgttg atgatgattg
     4921 gcgcatggag tattgcatcg caatgggatg ccggtgccaa tgcattaacg ctggcagcaa
     4981 ttagctgcgt actctactcc gccgtcgcag caccgtttaa gtcgttgtca ctgctgatgc
     5041 gcacgctggt gttactttcg ctattcagct ttgtggtcaa atttggcctg atggtccaga
     5101 ttagcgatct gtggcaattt ttactgtttc tctttccact gctggcgaca atgcagcttc
     5161 ttaaattgca gatgcccaaa tttgccgcac tgtgggggca actgattgtt tttatgggtt
     5221 cgtttatcgc tgtcactaat cccccggtgt atgattttgc tgattttctt aactataatc
     5281 tggcaaaaat cgttggcgtt gcgctggctt ggctagcgtt cgccattctg cgtccaggat
     5341 cggatgctcg taaaagccgc cgccatattc gcgcgctgcg ccgggatttt gtcgatcagc
     5401 taagccgcca tccaacactg agtgaaagcg aatttgaatc gctcacttat catcacgtca
     5461 gtcagttgag taacagccag gatgcgctgg ctcgccgttg gttattacgc tggggtgtag
     5521 tgctgctgaa ctgttctcat gttgtctggc aattgcgcga ctgggaatcg cgttccgatc
     5581 cgttatcgcg agtacgggat aactgtattt cactgttgcg gggagtgatg agtgagcgtg
     5641 gcgttcagca aaaatcactg gcggccacac ttgaagaatt acagcggatt tgcgacagcc
     5701 ttgcccgtca tcatcaacct gccgcccgtg agctggcggc aattgtctgg cggctgtact
     5761 gctcgctttc gcaacttgag caagcaccac cgcaaggtac gctggcctcg taattactta
     5821 attacaccac aggcatagcg ttcaccgcca ccgcccagcg gtttaggttg atcggacata
     5881 ttatcgccgc caacgtggac catcagcgcc ttgtctttga tttcatccag tgatttcaga
     5941 cgaggcgcga tgacggcatc ggtagctttg ccgtcattat tgacgaccag tgcaggcaga
     6001 tcgcctaaat gcccggtacc ttctggccct tcatgtttac cggtattttg tggatcaaga
     6061 tgcccgcctg cggattccgc ggcgctggct ttgccatctt tggtggctgg ctggcagctt
     6121 cctttggcat gaatatggaa gccatgttca ccggggggta atgctttcag atcgggtgaa
     6181 aactccagac ctttatcggt ttcagtaatg gtgacgctac caattgactg cccaacccct
     6241 tgcgacgtga cgaggttcat ctcgactttt tcactggcag cttgtgcgcc ggtcgcaaca
     6301 accagcgcca gaatggccag actaaaacgt ttcataggac ctccgttcat tgtgcattcc
     6361 tgaataagtg taaaccagtg acacattttt gaacggcggc tattcctaaa agtgcttacg
     6421 gtacgtcgta ccccagtgca gctttacgga tacgaaacca ttgttgacgg gtcattttca
     6481 gtgtttctgc ttcgacagct gcccgtacgc gctcaatttt acctgagccg ataattggca
     6541 gcggctgcga cggtaaacgt aatacccagg cgtaaaccac ctgttcaatc gagcccgcgt
     6601 ttaactcctc cgccaccaca gccagttcat cacgcagcgg ctggaaataa tcatcattaa
     6661 acagacgacc accaccaagg caggaccacg ccatcggacg aacacgcagt tgttgtagtt
     6721 ggtcgagcgt gccatccagc agtaacggct gatgcaccgg ggatatttcc acctgattag
     6781 tggcaagggt aaacggcaga cgtgattgca acagggcaaa ttgcgcaggc gtaaagttcg
     6841 atacgccaaa atgacgcact ttgccgctct gatgcagatg tttgaacgcg tccgccactt
     6901 catcggcatc cattaacggg tctggtcggt ggattaacag cagatccaga tgatcggtcg
     6961 cgagattaat tagcgactgt tcggcgctct taatgatgtg attgcggtca gtgatgtaat
     7021 gaccaatgac gttttcttca cgcgcggtcg tcgcgatacc gcatttactg acgatttcca
     7081 tccgttcacg caggtgaggt gccagtttca gtgcctcgcc aaacgccgct tcgcactgat
     7141 agccaccata aatatcagca tggtccacgg tggtcacgcc gagatccaga tgctcttcaa
     7201 taaaactgac cagctggcgg gcggacatat tccagtccat caatcgccag tagcccatca
     7261 caaaacggga aaactccggg ccttccggcg caatagtaat acgctgaacc ataatcgctt
     7321 cctcttatca gatataagag gagtatacgc aagattaggt tcaaaagagt gatggttgct
     7381 ccggttcgtc tgatgacgct ggcttatttg cgcgtaattt gcgcattaat cgctgccgac
     7441 aaaggcgcag cacctcttgt ttttcgccat cgctcatttt attccagtta aaacgctcat
     7501 cccgactacg aaaacagccg cgacaaaacc cgcgttcgtc agactggcaa attccccggc
     7561 acgggctctg gacgggaaag aactctaatt gctccgccac ctcgccctcc tcagataaga
     7621 ttattaccat tattgaagct gttaatgtcc aaagtagcaa ctttgcttgc actagaccaa
     7681 ctggtctact acactccaac gcatgaacaa acacaccgaa catgatactc gcgaacatct
     7741 cttggcgacg ggcgagcaac tttgcctgca acgtggattc accgggatgg ggctaagcga
     7801 attactaaaa accgctgaag tgccgaaagg gtccttctat cactactttc gctctaaaga
     7861 agcgtttggc gttgccatgc ttgagcgtca ttacgccgca tatcaccagc gactggctga
     7921 gttgctgcaa tccggcgaag gtaactaccg cgaccgcata ctggcttatt accagcaaac
     7981 actgaaccag ttttgccaac atggaaccat cagtggttgc ctgacagtaa aactctctgc
     8041 cgaagtgtgc gatctgtcag aagatatgcg cagcgcgatg gataaaggtg ctcgcggcgt
     8101 gatcgccctg ctctcgcagg ctctggaaaa tggccgtgag aaccattgtt taaccttttg
     8161 tggcgaaccg ctacaacagg cacaagtgct ttacgcacta tggcttggcg cgaatctgca
     8221 ggccaaaatt tcgcgcagtt tcgagccact ggaaaacgcg ctggcccatg taaaaaacat
     8281 tattgcgacg cctgccgttt agcaggcatt ttttatcacc agacgaccgg gagcctttat
     8341 gtcatctgaa aaactgtatt ccccactgaa agtgggcgcg atcacggcgg caaaccgtat
     8401 ttttatggca ccgctgacgc gtctgcgcag tattgaaccg ggtgacattc ctaccccgtt
     8461 gatggcggaa tactatcgcc aacgtgccag tgccggtttg attattagtg aagccacgca
     8521 aatttctgcc caggcaaaag gatatgcagg tgcgcctggc atccatagtc cggagcaaat
     8581 tgccgcatgg aaaaaaatca ccgctggcgt tcatgctgaa aatggtcata tggccttgca
     8641 gctgtggcac accggacgca tttctcacgc cagcctgcaa cctggcggtc aggcaccggt
     8701 agcgccttca gcacttagcg cgggaacacg tacttctctg cgcgatgaaa atggtcaggc
     8761 gatccgcgtt gaaacatcca tgccgcgtgc gcttgaactg gaagagattc cgggtatcgt
     8821 caatgatttc cgtcaggcca ttgctaacgc gcgtgaagcc gcttttgatc tggtagagct
     8881 ccactctgct cacggttatt tgctgcatca gttcctttcc ccttcttcaa accatcgtac
     8941 cgatcagtac ggcggcagcg tggaaaatcg cgcacgtttg gtactggaag tggtcgatgc
     9001 cgggattgaa gaatggggtg ccgatcgcat tggcattcgc gtttcgccaa tcggtacttt
     9061 ccagaacaca gataacggcc cgaatgaaga agccgatgca ctgtatctga ttgaacaact
     9121 gggtaaacgc ggcattgctt atctgcatat gtcagaacca gattgggcgg ggggtgaacc
     9181 gtatactgat gcgttccgcg aaaaagtacg cgcccgtttc cacggtccga ttatcggcgc
     9241 aggtgcatac acagtagaaa aagctgaaac gctgatcggc aaagggttaa ttgatgcggt
     9301 ggcatttggt cgtgactgga ttgcgaaccc ggatctggtc gcccgcttgc agctcaaagc
     9361 tgagcttaac ccacagcgtg ccgaaagttt ctacggtggc ggcgcggaag gctataccga
     9421 ttacccgacg ttgtaatcca acattgcgag cggcgtaaag ccgccgctat actaaaacaa
     9481 cattttgaat ctgttagcca ttttgaggat aaaaagatgc gtcttcttca taccatgctg
     9541 cgcgttggcg atttgcaacg ctccatcgat ttttatacca aagtgctggg catgaaactg
     9601 ctgcgtacca gcgaaaaccc ggaatacaaa tactcactgg cgtttgttgg ctacggcccg
     9661 gaaaccgaag aagcggtgat tgaactgacc tacaactggg gcgtggataa atacgaactc
     9721 ggcactgctt atggtcacat cgcgcttagc gtagataacg ccgctgaagc gtgcgaaaaa
     9781 atccgtcaaa acgggggtaa cgtgacccgt gaagcgggtc cggtaaaagg cggtactacg
     9841 gttatcgcgt ttgtggaaga tccggacggt tacaaaattg agttaatcga agagaaagac
     9901 gccggtcgcg gtctaggcaa ctaatctcct gccgggcgtg aactcatcgc gcccgcatct
     9961 ttactgcatc gacaagtaat atttgtcata atgcgcgctg caatttatcc gtattaagag
    10021 aatcagatgt ccgataacgc tcaacttacc ggtctgtgcg accgttttcg tggtttttat
    10081 cctgttgtga tcgatgttga aacagccgga tttaacgcca aaaccgatgc gctgcttgag
    10141 attgccgcca tcaccctgaa aatggatgaa caaggctggc tgatgccgga caccacatta
    10201 catttccacg tcgaaccatt tgtcggcgca aatttgcaac cagaagccct cgccttcaac
    10261 ggcattgacc cgaacgatcc cgatcgcggc gcggtcagcg aatacgaggc gctgcacgaa
    10321 atttttaaag ttgtacgtaa aggtattaaa gcgagcggct gtaaccgcgc cattatggtg
    10381 gcgcacaacg ccaattttga tcacagcttt atgatggccg ccgcagaacg cgcctcgctg
    10441 aaacgtaacc cgttccaccc tttcgccact tttgacactg ctgcactggc cgggctggca
    10501 ctcggacaaa ccgtattgtc aaaggcttgc cagaccgctg gcatggactt cgacagcacc
    10561 caggcgcact ccgcgctgta cgacaccgaa cgcactgctg tgctgttttg tgaaatcgtc
    10621 aaccgctgga aacgtctggg aggctggccg ctacctgccg ccgaagaggt gtaatcgagt
    10681 cgatgcctga tgacatgcaa tgattcaggc atctatagtg aggctattcc acgcatcaag
    10741 catgatattc acggggaata gcgttaatgg cagataatcc agacccttca tcgctcctgc
    10801 cggacgtgtt ttcaccggcg acccgcgact ggtttcttcg cgcctttaaa cagccgaccg
    10861 ctgtccagtc gcaaacctgg catgtggcgg cgcgaggcga acatgcgctg gtgattgcac
    10921 cgactggctc cgggaaaacg ctggcagcat ttctctacgc cctcgatcgg ctcttccgcg
    10981 aaggcggcga agatacccgc gaggcgcata agcgtaaaac ctcacgcatc ctctatattt
    11041 caccgataaa agccctgggc accgacgttc agcgcaactt gcagatcccg ttgaagggta
    11101 ttgccgatga acggcggcgg cgcggcgaaa cggtaattat tgatgaagtc cacgcggtag
    11161 cgggcagtaa acgtggtgcg catctggcgt taagtctgga gcggctcgat gcgctgctcc
    11221 acacctcagc acagcgaatt ggcctttctg ccactgtgcg ctcagccagc gatgtggcag
    11281 catttcttgg tggcgatcgc ccggttacgg tagtcaaccc gcccacaatg cgccatccgc
    11341 agatacgaat tgtcgtaccg gtcgccaata tggatgatgt ctcatcggtc gccagcggca
    11401 ccggcgaaga cagccatgcc ggccgggaag gctccatctg gccatatatt gaaacgggta
    11461 tccttgatga agtgttgcgc catcgctcga ccattgtctt tactaattca cgcgggctgg
    11521 cggaaaaact gacggcacga ttaaatgagc tttacgccgc acgcttacag cgttccccgt
    11581 ctatcgccgt tgatgcggcc catttcgagt cgacctccgg cgcaacctct aaccgtgtac
    11641 aaagtagcga cgtttttatt gcccgctcac accacggctc cgtctctaaa gaacaacgag
    11701 caatcaccga acaggcgctg aaatcgggtg aattacgctg cgtggtcgca acctccagtc
    11761 ttgaactggg gattgatatg ggcgcggtgg atctggtgat tcaggtggca acgccgcttt
    11821 ctgttgccag tgggttacaa cgcattggtc gcgccggaca tcaggttggc ggtgtatcta
    11881 aagggctgtt tttcccccgt acccggcgtg atttagtcga ttccgcagtc attgtagagt
    11941 gtatgttcgc aggcaggctg gaaaacctga caccaccgca taatcctctc gacgtccttg
    12001 cgcagcaaac cgttgccgcc gcggcgatgg atgcattaca ggtagacgaa tggtactccc
    12061 gcgtacgccg taccgcaccg tggaaagatc tgccaagacg tgtttttgac gccacgctgg
    12121 atatgctttc cgggcgctat ccctctggcg atttttctgc ttttcgcccc aaactggtct
    12181 ggaacaggga gaccgggata ttgaccgccc gacctggcgc tcaattgttg gcggttacca
    12241 gcggcggcac cattccggat cgtggcatgt atagcgtgtt attacccgaa ggtgaagaaa
    12301 aggccggttc gcggcgggtg ggtgaactgg atgaggagat ggtatatgag tcgcgggtga
    12361 acgacattat cactctcggc gctacctcat ggcggatcca gcaaatcacc cgcgatcagg
    12421 tgattgtgac tcctgctccg ggtcgttctg cccagctccc cttctggcgt ggtgaaggta
    12481 acggacgtcc ggctgaatta ggcgagatga tcggcgattt tcttcattta ctggcggatg
    12541 gcgcgttctt ttccgggact attcccccgt ggctggcaga agaaaatacg aacgccaata
    12601 ttcaggggtt gattgacgag caacgcaacg cgacgggcat cgttccgggg agtcgccatc
    12661 tggtcctcga acggtgccgt gatgaaattg gcgactggcg tattattttg cactctccct
    12721 atggaagacg ggtgcatgaa ccctgggcgc tggcgattgc cgggcgaata catgccctat
    12781 ggggcgctga cgcgtctgtg gtcgccagtg atgacagcat tgttgcgcgc attcctgaca
    12841 ctgatggtaa attgcccgac gccgcgattt ttttgtttta accagaaaag ttgctgcaaa
    12901 ttgtccgcga ggcggtaggc agctcggcac ttttcgccgc ccgttttcgc gaatgcgccg
    12961 cgcgggcatt attaatgccg ggccgcacgc cgggccatcg caccccgctt tggcaacaac
    13021 ggctgcgcgc cagtcagttg ctggaaatcg ctcagggata tccggatttt ccggtcattc
    13081 tcgaaaccct acgcgaatgt ctgcaagatg tttatgatct tcccgcactg gaacgtttga
    13141 tgcgtcgcct gaacggtggc gaaattcaaa tatccgatgt aacgaccact acgccctcgc
    13201 ctttcgccac aagtttattg ttcggctatg tcgcggaatt tatgtaccag agcgacgccc
    13261 cgctggcaga gcgccgggca tccgtactgt cgctggacag cgagttactg cgcaatctac
    13321 tcggacaggt cgatccgggg gaattactcg acccgcaggt cattcgccag gtggaagaag
    13381 agttgcaacg actggctcct ggtagaagag cgaaaggtga agaaggattg ttcgacctgc
    13441 tgcgcgaact ggggccaatg accgttgaag acctggcgca acggcataca ggcagcagtg
    13501 aagaggttgc gtcgtatctg gaaaatcttc ttgcagtaaa acgaatcttc ccagcgatga
    13561 ttagcggaca ggagcgtctt gcctgtatgg atgatgccgc caggctgcgt gatgccctcg
    13621 gcgtacggct accagagtca ttgccagaga tttatttaca tagagtcagt tacccgcttc
    13681 gcgacctctt tctgcgctat ctccgggctc atgctctggt cacggctgaa caactggctc
    13741 atgagtttag tctcggtatt gccattgtcg aagagcagct tcagcaactg cgtgaacagg
    13801 gtctggtgat gaatctgcaa caagacatct gggtgagcga tgaagtattt cgtcgtctgc
    13861 gtttgcgctc gctgcaagcc gccagagaag cgacgcgtcc cgttgcagcc acgacctatg
    13921 cgcgattgct gctggaacgt cagggcgtat tacccgccac cgatggtagc ccggcgctct
    13981 ttgcctcaac atctccaggc gtttatgagg gcgtagatgg agtgatgcgg gtgatcgaac
    14041 agcttgccgg agtcggttta cccgcctcac tctgggaaag ccagatcctg cctgcccgcg
    14101 tacgcgacta ttcgccagaa atgctcgatg aattactggc aaccggagcg gttatctggt
    14161 cgggacaaaa aaaagctggg tgaagatgac ggcctggtgg cactgcatct acagaaatat
    14221 gccgcagaat cgttcactcc cgccgaagcg gatcaggcga atcgttcggc gctgcagcaa
    14281 gcgataatcg ctgttctggc tgacggagga gcctggtttg cacaacaaat cagccaacgg
    14341 atacgcgata aaatcgtcga atcggttgat tcctcggcac tgcaagaggc gttatgggcg
    14401 ctggtctggc aaggcgtcat taccagcgat atttgggcac cgttacgcgc cctcacccgc
    14461 agcagttcca acgcacgcac ctcaactcgc cgcagtcacc gggctcgtcg tgggcgtcct
    14521 gtctatgcac aacccgtctc gccgcgggta tcttacaaca caccaaatct ggctggacgc
    14581 tggtcgttat tgcaggtgga gccactaaac gataccgaaa ggatgctggc gctggcggaa
    14641 aatatgctcg accgctacgg catcatcagt cgtcaggcgg tgatagccga aaatatccct
    14701 ggcgggtttc catcgatgca aacactttgt cgaagtatgg aagactccgg gcgaattatg
    14761 cgaggtcgtt ttgtagaagg tctgggtggc gcgcaattcg ctgaacgtct gactattgac
    14821 cgattgcgcg atctggcgac acaagccacg caaacacgcc actatacacc agtggcgctc
    14881 tccgccaacg atccggctaa tgtgtgggga aatcttctgc cctgggctgc acatccggca
    14941 acgctggtac caacgcgtcg ggcgggtgcg ctggtggtcg tttctggcgg caaattgtta
    15001 ctctatctgg cgcaaggcgg caaaaaaatg ctggtctggc aggaaaagga ggaattactc
    15061 gccccagaga ttttccaggc gctgaccacc gcactgcgtc gcgaaccaag gctgcgcttt
    15121 acgctaacag aagtgaatga tcttccggtc cggcaaacgc cgatgtttac gctgctgcgt
    15181 gaggcgggat tttcaagttc gccacaaggg ctggattggg gatagttgac atcctccacg
    15241 ccctgaaaga cgtggttccc tgctacgttc aagctgtcgc ctgaatcatt tcggtgggtt
    15301 cctgcttcaa cgagcggcct gactgcacca tctctccaca ggcaagaacg gcgtgccccg
    15361 ccgctaaaat gttacgagcg ccatttacat cggcgttcgc tgtatatcca catacctggc
    15421 atctgaattt actttgtgac aggcggtttt ctttcgctgt atgaccacag cacgcgcaac
    15481 gctggcttgt gtacgctggc ggaacagcaa gcacctgacc gccac
//
LOCUS       DAZOYF010000089        15452 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZOYF010000089 DAZOYF010000000
VERSION     DAZOYF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.089"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            447..1505
                     /gene="citC"
                     /locus_tag="ACHUOF_002618"
     CDS             447..1505
                     /gene="citC"
                     /locus_tag="ACHUOF_002618"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR0611748.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1520..1816
                     /gene="citD"
                     /locus_tag="ACHUOF_002619"
     CDS             1520..1816
                     /gene="citD"
                     /locus_tag="ACHUOF_002619"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR0611749.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1813..2721
                     /gene="citE"
                     /locus_tag="ACHUOF_002620"
     CDS             1813..2721
                     /gene="citE"
                     /locus_tag="ACHUOF_002620"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR0611750.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2732..4264
                     /gene="citF"
                     /locus_tag="ACHUOF_002621"
     CDS             2732..4264
                     /gene="citF"
                     /locus_tag="ACHUOF_002621"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR0611751.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4268..4818
                     /gene="citX"
                     /locus_tag="ACHUOF_002622"
                     /pseudo
     CDS             4268..4818
                     /gene="citX"
                     /locus_tag="ACHUOF_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4793..5671
                     /gene="citG"
                     /locus_tag="ACHUOF_002623"
     CDS             4793..5671
                     /gene="citG"
                     /locus_tag="ACHUOF_002623"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR0611752.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5722..7184
                     /gene="citT"
                     /locus_tag="ACHUOF_002624"
                     /pseudo
     CDS             5722..7184
                     /gene="citT"
                     /locus_tag="ACHUOF_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7298..8104
                     /gene="rna"
                     /locus_tag="ACHUOF_002625"
     CDS             7298..8104
                     /gene="rna"
                     /locus_tag="ACHUOF_002625"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR0611753.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8333..8743
                     /gene="rnk"
                     /locus_tag="ACHUOF_002626"
     CDS             8333..8743
                     /gene="rnk"
                     /locus_tag="ACHUOF_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR0611754.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8881..8970
                     /gene="yldA"
                     /locus_tag="ACHUOF_002627"
     CDS             8881..8970
                     /gene="yldA"
                     /locus_tag="ACHUOF_002627"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR0611755.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8974..10183)
                     /locus_tag="ACHUOF_002628"
                     /pseudo
     CDS             complement(8974..10183)
                     /locus_tag="ACHUOF_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            10404..10832
                     /gene="uspG"
                     /locus_tag="ACHUOF_002629"
     CDS             10404..10832
                     /gene="uspG"
                     /locus_tag="ACHUOF_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR0611756.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10953..12518)
                     /gene="ahpF"
                     /locus_tag="ACHUOF_002630"
     CDS             complement(10953..12518)
                     /gene="ahpF"
                     /locus_tag="ACHUOF_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR0611757.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12763..13326)
                     /gene="ahpC"
                     /locus_tag="ACHUOF_002631"
     CDS             complement(12763..13326)
                     /gene="ahpC"
                     /locus_tag="ACHUOF_002631"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR0611758.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13698..14444
                     /gene="dsbG"
                     /locus_tag="ACHUOF_002632"
     CDS             13698..14444
                     /gene="dsbG"
                     /locus_tag="ACHUOF_002632"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR0611759.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14654..>15452
                     /locus_tag="ACHUOF_002633"
     CDS             14654..>15452
                     /locus_tag="ACHUOF_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0611760.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKL"
BASE COUNT         3820 a         4003 c         3777 g         3852 t
ORIGIN      
        1 agatacgcag cggaaatgcc agtctttgga aaaatgcaaa ctgtttattc tcgttaagct
       61 gcaacattgt tttccttgcg ggttgtcccg agcctctttg acggatgtaa ttaaaatgtt
      121 gaatcaaaat acatagatat taattatgat cgtggcaaat tttattcatt aaatcaatta
      181 aatcacttaa acctgcgagt catttcgtaa ttacattaat taaaaaactt aaaaccatta
      241 aataaataaa aaaccactaa ctctatgtga aataaatcaa aatttcacgc cgaaatactc
      301 cttaggatgt atagcaaaag gagaaaaaga tatacctcga tcacccccct ttctcccaag
      361 tgaaaataaa aggttatcag tttgcaacat tgaacaacat tcgttgcaaa tcgataacaa
      421 catgcacctt caggatacta tttattatgt tcggcaatga tattttcacc cgcgtaaaac
      481 gttcagaaaa taaaaaaatg gcggaaatcg cccaattcct gcatgaaaat gatttgagcg
      541 ttgacaccac agtcgaagta tttattaccg taacccgcga tgaaaagctt atcgcgtgcg
      601 gtggaattgc cggaaatatt attaaatgcg ttgctatcag tgaatccgtt cgcggtgaag
      661 gactggcgct gacattagcc actgaactga taaacctcgc ctatgagcgg cacagcacgc
      721 atctgtttat ttataccaaa accgaatacg aggcgctgtt ccgccagtgc ggtttttcca
      781 cgctgacctg cgtacccggc gtgatggtgc tgatggaaaa cagcgccacg cgactgaaac
      841 gctatgccga atcgctgaaa aaatttcgtc atccagggaa caagattggc tgcattgtga
      901 tgaacgccaa tccctttacg aatggtcacc gttatctgat tcaacaagct gcggcacagt
      961 gcgactggtt gcatctgttt ttagtcaaag aagattcttc acgcttcccc tatgaagacc
     1021 ggctggatct ggtgttaaaa ggcaccgccg atattccacg cctgactgtg catcgcggct
     1081 cagaatacat catctcccgc gctacgttcc cttgctactt cattaaagaa cagagcgtca
     1141 ttaaccattg ttacaccgaa attgatctga agattttccg tcagtacctc gctcccgcgc
     1201 ttggtgtaac tcaccgcttt gtcggtactg aacccttttg tcgcgttacc gcccagtaca
     1261 accaggatat gcgctactgg ctggaaacgc cgactatctc cgcagcgccc atcgaactgg
     1321 ttgaaattga gcggctgcgt taccaggaga tgccgatatc cgcttcccgg gtacgtcaac
     1381 tgctggcgaa aaacgatctc acggctatcg cgccgctggt ccctgcagtc acgctgcatt
     1441 atttgcagaa cctgcttgag cactcccgcc aggacgcggc agctcgtcaa aagacccccg
     1501 catgagaaac aggtgaaaaa tgaaaataaa ccagcccgcc gttgcaggca cccttgagtc
     1561 tggggatgtg atgatacgca tcgccccact cgatacgcag gatatcgacc tgcaaatcaa
     1621 tagcagcgtt gagaaacagt ttggcgatgc aattcgcacc accattctgg acgttctcgc
     1681 ccgctacaac gtgcgcggcg tacagctgaa tgtcgatgac aaaggcgcac tggactgcat
     1741 tttacatgca cgactggaag ccctgctggc acgcgccagc ggtatcccgg ctctgccatg
     1801 ggaggattgc caatgatttc cgcttcgctg caacaacgta aaactcgcac ccgccgcagc
     1861 atgttgtttg tgcctggtgc caatgccgcg atggtcagca actccttcat ctacccggct
     1921 gatgccctga tgtttgacct cgaagactcc gtggcattac gtgaaaaaga caccgcccgc
     1981 cgcatggttt atcacgcgct gcaacatccg ctgtatcgcg atattgaaac cattgtgcgt
     2041 gtcaacgcgc tggattccga atggggtgtt aacgacctgg aagccgtcgt tcgcggtggt
     2101 gcggacgttg tgcgtctgcc gaaaaccgat accgctcagg atgttctgga tattgaaaaa
     2161 gagatcctgc gtatagaaaa agcctgtggt cgtgaacccg gcagcaccgg cctgctggcg
     2221 gcgattgaat ctccgctggg cattacccgc gcagtggaaa tcgctcacgc ttccgagcgt
     2281 ttgatcggta tcgccctcgg tgcagaagac tatgtgcgca acctgcgtac agaacgctcc
     2341 ccggaaggaa ctgaactgct gttcgcacgc tgttccattt tgcaggccgc gcgctctgcg
     2401 ggtattcagg cgttcgatac cgtctattcc gacgctaaca acgaagccgg attcctgcaa
     2461 gaagccgccc acatcaaaca gctgggcttt gacggcaaat cgctgatcaa cccgcgtcag
     2521 attgatctgc tgcacaacct ctacgcaccg acccagaaag aagtggatca cgcccaccgc
     2581 gtcgtagaag ccgctgaagc cgccgctcgc gaaggcctcg gcgtggtttc cctgaacggc
     2641 aagatggtgg acggtccggt tatcgatcgc gcccgtctgg tgctctcccg tgcagaactt
     2701 tccggcatcc gcgaagaata aggcaatcaa aatgacgcag aaaattgaac aatctcaacg
     2761 acaagaacgg gtagcggcct ggaatcgtcg cactgaatgc gatcttgccg ctttccagaa
     2821 ctcaccaaag caaacctacc aggctgaaaa agcgcgcgat cgcaaactgt gcgccaacct
     2881 ggaagaagcg attcgtcgct caggtttaca ggacggcatg acggtttcct tccatcacgc
     2941 tttccgtggc ggtgacctga ccgtcaatat ggtgatggac gtcatcgcga agatgggctt
     3001 taaaaacctg accctggcgt ccagctccct gagtgattgc catgcgccgc tggtagaaca
     3061 cattcgccag ggcgtggtta cccgcattta tacctctggc ctacgcggtc cactggcgga
     3121 agagatctcc cgtggtctgc tggcggaacc ggtgcagatc cactctcacg gcggtcgtgt
     3181 gcatctggta cagagcggcg aactgaatat cgacgtggct ttcctcggcg tcccgtcctg
     3241 tgatgaattc ggtaatgcca acggctacac cggtaaagcc tgctgcggct ccctcggcta
     3301 tgcaatggtt gatgccgaca acgcaaaaca ggtcgtgatg cttaccgaag aactgctgcc
     3361 ttatccgcat aatccggcaa gcattgagca agatcaggtt gatttgatcg tcaaagttaa
     3421 tcgcgttggc gatgctgcaa aaatcggcgc tggtactacc cgtatgacca ctaacccgcg
     3481 cgaactgctt attgcccgta gcgctgcgga tgtgattgtc aactctggct acttcaaaga
     3541 aggtttctcc atgcaaaccg gcaccggcgg cgcttcgctg gcggtaaccc gtttcctgga
     3601 agacaaaatg cgtagccgcg atattcgcgc cgacttcgcc ctcggcggta ttaccgcgac
     3661 gatggttgat ctgcacgaaa aaggtctgat ccgcaaactg ctggatgtgc agagctttga
     3721 cagccatgct gcgcaatcgc tggcccgtaa ccccaatcac atcgaaatca gcgccaacca
     3781 gtacgctaac tggggttcga aaggcgcatc ggttgatcgt ctcgacgtgg tggtactgag
     3841 cgcgctggaa attgacaggc agttcaacgt taacgtgctg accggttctg acggcgtact
     3901 gcgtggcgct tccggtggtc actgcgatac cgcgattgcc tctgcgcttt ccatcatcgt
     3961 cgcgccactg gtacgcggtc gtattccgac tctggtggat aacgtgctga cctgcatcac
     4021 cccgggctcc agtgtcgata ttctggtcac agaccacggt atcgcagtta acccggcacg
     4081 tccggaacta gcagaacgtc tgcaggaagc gggcattaaa gtggtttcca ttgagtggct
     4141 gcgcgaacgt gcgcgtctgc tgaccggtga accacagccg attgaattca ccgaccgcgt
     4201 cgttgccgtt gtgcgttacc gcgatggctc ggtgatcgat gttgtgcatc aggtgaagga
     4261 ataagccatg cacctgcttc ctgaactcgc cagccaccat gcggtgtcaa ttcccgagct
     4321 gctcgtcagc cgggatgaaa ggcaagcacg gcaacacgcc tggctcaagc accatcctgt
     4381 tccactggtc tcctttaccg tggttgcgcc tgggccgatt aaagacagcg aggtcacacg
     4441 ccgaattttt aatcatcgcg tgacagcctt gcgtgcctta gccacaaagc agggctggca
     4501 aattcaggag caggctgcac tggtttccgc cagcgggccg gagggcatgt tgagcattgc
     4561 cgccccggct cgcgacctca agctcgccac cattgagctt gaacatagtc atcctctcgg
     4621 gcggttatgg gatatcgatg tcctgacgcc cgaaggcgaa attctctccc gccgcgacta
     4681 ttcactgcgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4741 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4801 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4861 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4921 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4981 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     5041 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5101 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5161 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5221 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5281 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5341 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5401 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5461 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5521 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5581 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5641 gatccttacc tgttttttag cacagattta attatttaag cacttgataa atttggaaat
     5701 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5761 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5821 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5881 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5941 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     6001 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6061 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6121 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6181 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6241 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6301 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6361 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6421 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6481 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6541 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6601 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6661 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6721 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6781 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacttggaag
     6841 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6901 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6961 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     7021 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7081 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7141 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7201 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7261 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7321 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7381 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7441 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7501 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7561 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7621 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7681 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7741 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7801 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7861 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7921 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7981 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     8041 cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8101 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8161 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8221 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8281 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8341 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8401 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8461 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8521 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8581 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8641 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8701 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8761 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8821 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8881 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8941 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     9001 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9061 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9121 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9181 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9241 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9301 aacaatgccg caacgcttta ccgctgctgc cctccagttt caggttagtc agcaccgttt
     9361 ccgtggtgct gcctttcgct tcaaaaccga cggcgtctat tactgcatcc acgccccggt
     9421 gacctgccgt ttgttcaata attgactgtg ccggatcgct atcttcatcg aaattaatcg
     9481 ggatcgcgcc gtagcggtca gcggcgaaac gcaagcggta gggatgatga tcaacaacaa
     9541 aaatctgttc cgcaccgagc aaccgtgcac aggcgattgt caacaatccc acaggaccag
     9601 caccatagac tgcaacgctt gaaccttgtt ggatctgcac attttttgct gcctgccatg
     9661 ccgttggcag aatatcagaa aggaaaaggg ctttatcatc tgaaagcaaa ggcggtactt
     9721 taaacggccc cacattccct ttcgggacgc ggacatattc cgcctgcccg ccaggaacgc
     9781 caccatacag gtgactataa ccaaacaatg ccgcgggagc aggtatctgt tttttattga
     9841 gcgcagcgcc tttacccgca ttggtatttt cgcaggcggc atattgctgt aatcgacaga
     9901 aaaaacagtc gccacaagca atgacgaacg gaattaccac ccggtcgcct ttttgcaaat
     9961 tttttacgtc cttcccggtt tcaacgactt cccccataaa ttcatgacca aaaatatcgc
    10021 catgtttaac ctggggtatt ttgcctcgat aaagatggag gtcagagcca cagatcgccg
    10081 ttaccgtaat acgcagaata atatcatctg cctgttcaat gcccggatcg ggaacatttt
    10141 ctacctgaac gtgatgtggg ccgtgataag tcaatgcttt catgcaacct ccagagacag
    10201 ttcaaaaaag agtcagtctt taagggtagc tatcacagcg ggaaaacggg cggtaccgca
    10261 ggttttggga gaattcctag tgttcagtga gaatatatta atcacagaaa ccgttcgtaa
    10321 ggaaaaagaa ccagacaaat atcagggata aatattataa tcatggagaa tgatttgttc
    10381 atgattaaca gggagaaagg gttatgtata aggcaatcat tatgccagtt gatgtatttg
    10441 aaatggaatt gagcgacaaa gctgttcgcc acgctgaatt cctcgcccag gatgacggag
    10501 ttattcatct acttcacgta ctacccgggt cagccagcct gagcctgcac cgttttgccg
    10561 ctgatgtgcg tcgttttgaa gagcatctgc aacatgaagc agaagaacgt ctgcaaacga
    10621 tggtcagcca cttcaccatc gatccttccc gcattaaaca acatgtccgt tttggtagcg
    10681 tgcgggatga agtcaatgag ttggcagaag aactgggggc tgatgttgta gttatcggtt
    10741 ctcgcaaccc atcgatttcg acccatctgt taggttctaa cgcctcgagc gtgatccgcc
    10801 acgccaatct gcctgtactg gtcgttcgct aacagataaa agtatttatg ccgccctgcc
    10861 tggaacatcc cggcagggct ttttaatgcc ataaaaaagc cgccaggttt gaccccggcg
    10921 gctaagcaat tgcaggtgaa tcttacttct tcttatgcag ttttggtgcg aatcaggtag
    10981 tcaaaagcac tcagagaggc tttggcacct tcgccagtgg cgatgatgat ctgcttgtac
    11041 ggaaccgtcg tacagtcacc cgctgcgaac acgcctttga cgttggtttc acatttcgca
    11101 tcaatgataa tctcgcccat gcggttacgt tcgactgcgc cttccagcca gttggtgttc
    11161 ggcagcagac caatctggac aaaaataccg gccagttcga tgttgtgaat attgccgctg
    11221 acacgatctc gatattccag accaacgact ttgctgccgt cgcctttcac ttccgtggtt
    11281 tgcgcattca gaataatgtc gacgtttttc aggctgcgca gtttgtcctg cagaacctgg
    11341 tcggctttca tttctggcgc aaattccagc agcgttacgt gctcaacgat acccgccagg
    11401 tcgattgccg cttccacgcc ggagttaccg ccgccgataa ccgctacgcg tttgccttta
    11461 aacagcgggc cgtcgcagtg cgggcagtag gtcacgcctt tagtgcgata ctgatcttcg
    11521 cccggaacgt tcatgttgcg ccattttgca cccgtcgcca cgataatgct gcgtgctttc
    11581 agtaccgcgc cagaagctgt ttcaatctgg tgtagaccac cttcaaccgc tgccgggatc
    11641 agtttgctgg cgctctggct gtcgatcaca tcaacgtcgt attcatcaac gtgaactttc
    11701 agtgcgcctg ccaacttctg cccttcagtc ttcggtacag aaatgtagtt ttcgatatca
    11761 acggtatcga ggatctgacc accaaaacgt tcgcccatca gaccggtacg gatgccttta
    11821 cgtgcggagt aaattgctgc cgctgcaccc gccgggccgg aaccaacgat taatacgtca
    11881 taagcatcac gcttgttcag ctcttctgcc gcacgatttt ccgcgccagt atcaattttg
    11941 gcaacgattt cagtcaacgt catgcggccc tgaccaaact ctttcccgtt tacgaacact
    12001 gccggaacgc ccatcacgtt gcgatcggtg atttcgttct ggaaggtgcc gccgtcaatt
    12061 gcagtgtgct tgatgcgcgg gttcaatacg ctcatcaggt tcagcgcctg caccacgtcc
    12121 gggcagttat ggcaagagag cgagtaatag gtttcgaatt caaaatcacc gtcaatatgg
    12181 cgaatctgct ccagcagaga ctgcgcttct ttcgacggat gaccaccggt ccacagcaac
    12241 gccagtacca gcgaggtgaa ctcgtggccc agcggggagc ctgcaaaacg tggtccctgg
    12301 ttggaacctg ggttggtgat caggaaagac ggcttacgca ctggcaagct gttatcttct
    12361 ttaaaggtga ctttgtctga cagttctgcg atttcagcca acagttcctt gatttctgcc
    12421 gatttagcgc tgtcatccag cgtggcaatt aactcaacag gcttggtcaa tttctcaagg
    12481 taagccttga gttgagtttt catatttgtg tcgagcatgt ttatctcctg ggctttaaac
    12541 atcatcatgc aagcggcctc tgcggctacc tggatgcagc ttgcatcatg gtgcggggaa
    12601 gaaaatcggg tgccgcagca cccgaagaat taggtgaatt tccgaagtat ttcgcgtcag
    12661 agcaaggcga ctagcctgtg aatccccggg agcttacaca agtaagtgac cggggtgagc
    12721 gggcgacgcc aacgccgcta tggcgtgaaa gacgaaggaa atttagattt taccaaccag
    12781 gtccagagac ggagccagag ttgcttcacc ttctttccat ttagccgggc aaacttcacc
    12841 tgggtgagaa gctacgtact gagctgcttt gattttacgc agcagatcag acgcgtcacg
    12901 gccaatgcct tcagcggtaa cttcgattgc ctggatgata ccctgcgggt caacaacgaa
    12961 ggtcgcacgg tcagccagac cttcatcttc acgcatgttg tcgaagttac gggtcagggc
    13021 gccagtcggg tcgccgatca tcgcatattt gattttagcg atggtttcag agctgctgtg
    13081 ccatgctttg tgggtgaagt gagtatcggt agatactgcg tatacgtcta cgcccagttt
    13141 ctgcagttct tcgtagtggt cagcaacgtc acccagttcg gtcgggcata cgaaagtaaa
    13201 gtcagccggg tagaagaaga agacgctcca gcggccttcg gtatcttttt cggtgatttc
    13261 gatgaattcg ccgtttttga atgcctggtt tttaaaaggt ttaattttgg tgttaatcaa
    13321 ggacatctat acttcctccg tgttttcgat gagatgtaag gtaaccaatt tttgccgatt
    13381 cggctaatgc gtttccatta tcaaatcgat aagttttacc ttacaacctt cgtaagacaa
    13441 ctttgtgaac tttgcaccgc cagcggcgat aaaaagtaaa atggccccct ttccaggtga
    13501 ccgtgtaatc tgaactaccc tttggtatct tcagcgcctg cctgcaggct gaggtgttgc
    13561 ccttacaaat gcaacaacga catggattac aacaccctca taaacaaagg gcaatcacct
    13621 gatctaagct cttacctatg acagtgatag gttatgcctt ttactcgact tttgcactga
    13681 ctgaaaagaa caaattaatg ttaaaaaaga tacttttact ggctctgctt cctgcaatcg
    13741 ccttcgcaga ggaacttcct tccccagtaa aagcgattga aaaacagggc attacaatca
    13801 tcaaaacgtt cgatgccccc ggaggaatga aaggttatct cggaaagtat caggatatgg
    13861 gcgtcaccat ctacctgact cccgatggta agcacgctat ctctggttac atgtacaacg
    13921 agaaaggtga aaacctgagt aacacactta tcgaaaaaga aatttacgca ccagccggac
    13981 gcgaaatgtg gcaacggatg gaacaatccc actggctcct cgacggtaaa aaagatgcgc
    14041 cggtcattgt ctacgtcttc tccgatccgt tctgcccata ttgtaaacag ttctggcagc
    14101 aggcgcgccc gtgggtagat tctggcaaag tgcaattaag aacattgttg gttggggtta
    14161 tcaagccaga aagcccggcg acagcagcgg caattcttgc ctccaaagat cccgcaaaaa
    14221 cctggcaaca atatgaagcc tctggttgca agcttaagct aaacgtgcct gcaaacgtaa
    14281 gtacagagca aatgaaagtg ttaagtgaca atgagaaact gatggacgat ctgggggcaa
    14341 atgtcacgcc ggctatctat tacatgagta aggaaaatac gctgcaacag gccgtggggt
    14401 tgcccgatca gaaaacgctt aatatcatta tgggaaataa ataaaaggta attcatggat
    14461 ggttgaacta tatccctgct tttcaaccat cctaaaaaag catgaatttc tcgctctacc
    14521 ctaagtggct gtgctatttt agaaaaactt aatcctgagt taagcaaaaa aacataatca
    14581 ataaaaatac atacgcaaaa aacattgatt aagtgaatat accatggaag aaaaatataa
    14641 accggagtag tgtatggcca atctctacga cttgaaaaag ttcgacttaa atcttcttgt
    14701 catatttgag tgtatttacc agcatttaag cattagcaaa gctgctgaat cactatatat
    14761 caccccatca gccgtgagcc agtcgttaca acgcttacgt gcgcaattta atgacccttt
    14821 attcattcgc tcaggaaaag gtatcgcgcc gacgacaaca gggttgaatc tgcatcacca
    14881 tcttgagaaa aacctcaaag gccttgaaca aacaattaat atagtcaata agtccgaact
    14941 aaagaaaaac tttattatct acggccctca gctcatttct tgctctaata atagtatgct
    15001 gatccgctgc ctacggcaag atgcctctgt cgagattgag tgccatgata tcctcatgtc
    15061 agcagagaat gctgaagaac tgttggttca ccgcaaagcc gatctggtga tcacccaaat
    15121 gccagtgatc agccgttccg tcatttgcat gccattgcat acaattcgca atactctaat
    15181 ttgtagtaat aagcatccgc gaattactga caatagcact tatgaacaaa ttatggcaga
    15241 agagttcacc caattaatag ccaaatcagc aggagttgat gatattcaaa tggaaataga
    15301 cgagaagttt atgaatcgca aaatttcatt tcgtggttcc tctttgctga caataattaa
    15361 tagcattgca gttacagatt tactgggaat agtcccctat gaactttata actaccaccg
    15421 tgactttctt aatttaaaag agataaaact ag
//
LOCUS       DAZOYF010000090        15412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZOYF010000090 DAZOYF010000000
VERSION     DAZOYF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.090"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1177)
                     /locus_tag="ACHUOF_002634"
     CDS             complement(<1..1177)
                     /locus_tag="ACHUOF_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0611761.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="ACHUOF_002635"
     tRNA            complement(1376..1460)
                     /locus_tag="ACHUOF_002635"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="ACHUOF_002636"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="ACHUOF_002636"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR0611762.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="ACHUOF_002637"
     CDS             2804..4306
                     /locus_tag="ACHUOF_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR0611763.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHUOF_002638"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHUOF_002638"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR0611764.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHUOF_002639"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHUOF_002639"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR0611765.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHUOF_002640"
     CDS             complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHUOF_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR0611766.1"
                     /translation="MSIIILATISTTQENK"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="ACHUOF_002641"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="ACHUOF_002641"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR0611767.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="ACHUOF_002642"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="ACHUOF_002642"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR0611768.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="ACHUOF_002643"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="ACHUOF_002643"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR0611769.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..13067)
                     /locus_tag="ACHUOF_002644"
     CDS             complement(11871..13067)
                     /locus_tag="ACHUOF_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR0611770.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13260..13763
                     /locus_tag="ACHUOF_002645"
     CDS             13260..13763
                     /locus_tag="ACHUOF_002645"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0611771.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="ACHUOF_002646"
     CDS             complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="ACHUOF_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR0611772.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14387..15400
                     /gene="argF"
                     /locus_tag="ACHUOF_002647"
     CDS             14387..15400
                     /gene="argF"
                     /locus_tag="ACHUOF_002647"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR0611773.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3978 a         3933 c         3968 g         3533 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta
    12841 acaaaaacat tcccaccggt aattccataa gaaaatcgtt gtccgttaac ttccatatta
    12901 gcataaagat aacgcttaca tttcattaat gcccatggta aatagatacc tagcgttata
    12961 atcgttaaca acacattcac cacacaaata agaaaatatc tctcacctgt accatgaaaa
    13021 acaaaagaat gggacggaac atccatttca ttaataactt gagccataaa aatcccctga
    13081 attaaaaaat ccattgatat aatttataga tacagacagt aaaatgttct gccacgttac
    13141 aatactctta tcagtaatag gctggcaaaa actttattgc tttataagac gcttgcgcac
    13201 gtaagaagag agtggtatta aacaaatgta taattacttt atttttattg ggttaaacta
    13261 tgaataacat tgcgccgcaa tcacctgtaa tgcgtcgcct gacgctgcag gataatcctg
    13321 ctatcgccag cgtcatccgt caggtttacg cagagtatgg tcttaccgcc gataaaggct
    13381 acaccgttgc cgatccgaat ctcgacgaac tgtatcaagt gtacagccag cctggccatg
    13441 cttattgggt ggttgaatac aatggcgaag tggtcggcgg cggcgggatt gcgccattaa
    13501 caggtagtga agcggacatt tgcgaactgc agaaaatgta ctttcttccc gctattcgcg
    13561 gtaaagggct agcgaaaaaa ctggccttaa tggcgatgga gcaggcacga gagatgggct
    13621 tcaaacgctg ctatctggaa acgaccgctt ttttaaagga agccattgcg ctttatgagc
    13681 atttgggctt tgagcatatc gactatgcgc ttggctgcac gggccatgtc gattgtgaag
    13741 tgcggatgct acgcgaactc taaaaaaatg cccgctagta tgtgctatcg ggcattttaa
    13801 tcaattaatt agtggcgaac cccgtcatcg tcttcatcga caaaatcttc atcgtcgcca
    13861 tcttcgccgt taggatcttc aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc
    13921 tccgccagcg tcatcagttg ttcaacctgg gcatcgatca gatcggcatt caacgcgcac
    13981 tcgctgagga tgtcgcagca aatcacgata tcaccatctt caacttccag ctcttctgga
    14041 tcggtcactt cgtaaccgag tttaaacgct tcaactgccg ctttttccag agtttccaga
    14101 tcgtctgcgg aaagatggtg ttcgatggtg tacagcgcgt ccggatcgct gccatcttcc
    14161 agtaattctt caataatcaa acgtgtttct tcacgctgtt cttccagttg ttccgggttt
    14221 gccatggctc attcctctta atgtcctgcc gatactctta ttgtcacaca ccactgacat
    14281 tgcctccacc tttgtgacaa agatttatgc tttagacttg caaatgaata ataatacaca
    14341 taaattgagt tttaattcat tgaggcgtta gccacaggag ggatctatgt ccgggtttta
    14401 tcataagcat ttcctgaaat tactcgattt cacgccagct gaactcaaca gcctgctgca
    14461 gttagccgcg aagctgaaag ctgataagaa aagcggtaaa gaagaagcca aactcactgg
    14521 taaaaacatc gcgctcatct tcgaaaaaga ctcgactcgt acccgatgct ctttcgaagt
    14581 tgccgcatat gaccaggggg ctcgcgttac ttatcttggc ccaagcggta gtcagattgg
    14641 tcataaagag tcgattaaag acactgcccg cgtgcttggt cgcatgtatg acggtattca
    14701 gtatcgcggc tatggtcagg agattgtcga aacactggcg gaatacgctg gcgtgccggt
    14761 atggaacggc ctgaccaatg agtttcaccc cacgcagttg ctggcggatc tcctcaccat
    14821 gcaggagcat ttacccggta aagcattcaa cgaaatgacg ctggtctatg caggtgacgc
    14881 acgtaacaac atgggcaatt cgatgctcga agctgcggcg cttaccggtc tggatttgcg
    14941 tctggtcgcg ccacaggcat gctggccaga agctgcgctg gttacggaat gccgcgccct
    15001 ggcacaacaa aatggcggga atattacgct gacggaagat gtagctaaag gagttgaagg
    15061 tgctgacttt atctataccg atgtatgggt gtcgatgggt gaagcaaaag aaaaatgggc
    15121 tgaacgcatt gcattactgc gtgattatca ggtgaacagc aagatgatgc agttgaccgg
    15181 taacccggag gtcaaattcc tccattgcct gcccgcgttt catgacgacc aaacgacgct
    15241 tggcaaaaaa atggcggaag agtttggcct acatggcgga atggaagtga ctgatgaggt
    15301 cttcgaatct gccgccagca ttgtatttga tcaggcggaa aaccgcatgc ataccatcaa
    15361 agcggtgatg gtcgcgacgc tcagtaaatt aaataattaa tatctctata aa
//
LOCUS       DAZOYF010000091        15380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZOYF010000091 DAZOYF010000000
VERSION     DAZOYF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.091"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            41..604
                     /locus_tag="ACHUOF_002648"
     CDS             41..604
                     /locus_tag="ACHUOF_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611774.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            complement(877..1824)
                     /locus_tag="ACHUOF_002649"
                     /pseudo
     CDS             complement(877..1824)
                     /locus_tag="ACHUOF_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(1824..2417)
                     /locus_tag="ACHUOF_002650"
     CDS             complement(1824..2417)
                     /locus_tag="ACHUOF_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFR0611775.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            2564..2971
                     /locus_tag="ACHUOF_002651"
     CDS             2564..2971
                     /locus_tag="ACHUOF_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFR0611776.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(3091..4083)
                     /gene="rpoS"
                     /locus_tag="ACHUOF_002652"
     CDS             complement(3091..4083)
                     /gene="rpoS"
                     /locus_tag="ACHUOF_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR0611777.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(4146..5240)
                     /gene="nlpD"
                     /locus_tag="ACHUOF_002653"
     CDS             complement(4146..5240)
                     /gene="nlpD"
                     /locus_tag="ACHUOF_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR0611778.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(5425..6051)
                     /gene="pcm"
                     /locus_tag="ACHUOF_002654"
     CDS             complement(5425..6051)
                     /gene="pcm"
                     /locus_tag="ACHUOF_002654"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR0611779.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(6045..6806)
                     /gene="surE"
                     /locus_tag="ACHUOF_002655"
     CDS             complement(6045..6806)
                     /gene="surE"
                     /locus_tag="ACHUOF_002655"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR0611780.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(6787..7836)
                     /gene="truD"
                     /locus_tag="ACHUOF_002656"
     CDS             complement(6787..7836)
                     /gene="truD"
                     /locus_tag="ACHUOF_002656"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR0611781.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(7833..8312)
                     /gene="ispF"
                     /locus_tag="ACHUOF_002657"
     CDS             complement(7833..8312)
                     /gene="ispF"
                     /locus_tag="ACHUOF_002657"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR0611782.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(8312..9022)
                     /gene="ispD"
                     /locus_tag="ACHUOF_002658"
     CDS             complement(8312..9022)
                     /gene="ispD"
                     /locus_tag="ACHUOF_002658"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR0611783.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(9041..9352)
                     /gene="ftsB"
                     /locus_tag="ACHUOF_002659"
     CDS             complement(9041..9352)
                     /gene="ftsB"
                     /locus_tag="ACHUOF_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR0611784.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(9546..9869)
                     /locus_tag="ACHUOF_002660"
     CDS             complement(9546..9869)
                     /locus_tag="ACHUOF_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR0611785.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(9919..10524)
                     /gene="cysC"
                     /locus_tag="ACHUOF_002661"
     CDS             complement(9919..10524)
                     /gene="cysC"
                     /locus_tag="ACHUOF_002661"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR0611786.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(10524..11951)
                     /gene="cysN"
                     /locus_tag="ACHUOF_002662"
     CDS             complement(10524..11951)
                     /gene="cysN"
                     /locus_tag="ACHUOF_002662"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR0611787.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(11953..12861)
                     /gene="cysD"
                     /locus_tag="ACHUOF_002663"
     CDS             complement(11953..12861)
                     /gene="cysD"
                     /locus_tag="ACHUOF_002663"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR0611788.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            13113..14150
                     /gene="iap"
                     /locus_tag="ACHUOF_002664"
     CDS             13113..14150
                     /gene="iap"
                     /locus_tag="ACHUOF_002664"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR0611789.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(14296..15376)
                     /locus_tag="ACHUOF_002665"
                     /pseudo
     CDS             complement(14296..15376)
                     /locus_tag="ACHUOF_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
BASE COUNT         3770 a         4107 c         3787 g         3716 t
ORIGIN      
        1 tttattattt atcaatgaat tagatagaaa ggaaccaaga atgagaaaag caccccaagg
       61 ttcttttcct gccgatggaa aagaaccgat tatcgaaaga atctttaggt tagtagaacg
      121 atacccttca agaaatgcgg ctgctcgggc atgggggatc aatgaaggca ctttaaaaaa
      181 ttattacaac aggcgggatt acgcaccgat accacgcccg tatcagttaa aaaagatagc
      241 agaaagcgaa aatgttacct tggaatggct catgacggga gagggtgatg ttgaactaaa
      301 agattccaaa gaacccaaaa aagagaatgc ggataatgtt gatgctcaaa ttctaatgtt
      361 cttgtcattt cttaaaccac aagaaaaaca acaagtagct gatgttcttg ggagaaaagg
      421 agcggagcaa ttactcattc ttctggatga gcacatccaa gagttgcacg ctctagttgg
      481 agttcgtaga gccatagcgt taagccttgg taatttgcca gacgaaaagg ttagagagat
      541 ttacgcgttg tccgaagcta agggcgatca cttaaatcta cctcagaaag cagccagcgc
      601 ataacccagt gcaagtgcct agctgtgcta aaattacatt ggttttggcg cttgcaccca
      661 tcagcgctat aataaacacc tgaatcgttt ttgaacggtc tttaaaggct attgtaagaa
      721 cctgtgagtt atcttgaatg ttttattgat atcgaaccgt ttaacatttt tggactcgct
      781 ttatcttgaa aaccttgagt tcctaacttg ttgattccaa tctattcccg tcttttttaa
      841 tctcttcccg gttatcttga ataaattacc tagtcatagg tagtcgattt cggtccacgg
      901 cataccgaga tagagtgatt cgaaaatcgg tttgctgcga taagagactt tatcgatgcg
      961 caccacggtc atgttgcgcc caccggagta gtgcccggta aactcaccga acggcccttc
     1021 gatttcgcgt ttacggcttt cgataacccc ttcgaggatc acttctgaac cccacggcac
     1081 atcaaaaccg gtcagcggag cggtggcgat cgggtacggg ctttcacgca gcgcgcctgc
     1141 catttcatac tcagactgat cgtatttcag cggcgtggcc cccatcagcg tgatgatcgg
     1201 atcgttaccg agcgtgatcg caatcggcag atcttcaccg cgctcttctg ctttatgcag
     1261 atgcagggcg atatcgtgca tcggcaccgg ttgcaggccg agcttacgct tgcccttcac
     1321 ttccatgcgg tagatgccga cgttctgctt gccgaagtta tccgggtcga gcggatcgcg
     1381 ggaaaccacg cacgctttgt cgagatagaa accgccatcg ccatcattca ggcgaaacag
     1441 cggcagaata tcgaacaggt tgatctcttc gccatcgacg gtgttctgcg cccaggctgg
     1501 attggcgcgg cgctccgggg caatcgggaa gttatcccag cggcggataa actcatcaat
     1561 ctgctttttg accggggtgt ttggtggcag gccaagggaa atcgcgtggt tctgccagga
     1621 accgatggta ttcatcgcca cgcgggcatc ggtaaagccg cgaatattat caaaccacag
     1681 cgcgggcgcg ccgtcgccga tacgcccggt ggcgttagct gctgctgcca gatccggctc
     1741 agcgttcact tcttcgctga ttttcagtaa ctggccgtgg tcatcaagcg cctgtaaaaa
     1801 gctgcgtaaa tcatcaaatg ccattattca ttctcctgag aaaaattccg ggcctgcggc
     1861 aatccttgcc agcgcctggc gtggggatgt tcgaggccaa attgatccag cacgcgggct
     1921 accacatggt ggacaatgtc atctaccgtt tcgggatggt tataaaaggc aggcatcggc
     1981 ggcaccatcg ccacgcccat gcgtgaaagt gcgagcatat tttcgagatg gatggtgcta
     2041 agcggcattt cacgcggcac cagcaccagt ttgcggcctt ctttgagcac gacgtccgcc
     2101 gcgcgcccta ccaggccatc ggcgtaacca gcgcggatac cggcgagcgt tttcatactg
     2161 cacggaataa cgatcatgcc gtcggtacga aaagaaccgg atgagatgat cgccgcctga
     2221 tccgccgggt tatggctgaa gtctgcgagg gcagcaacat cgcgagcgct gtaaggcgtt
     2281 tccagttcaa tggtggtttt cgcccacttc gacatcacca gatgagtctc gacattcggc
     2341 atctcacgca gcgcttgcag taatgccaca ccaagaggcg caccggtagc ccctgtcatc
     2401 ccgacgatca gtttcattgc ttattctctt aattattcgt atacgaacgt tattattaac
     2461 atactccttc atttccacag cggcaaggat tgaaaagtaa caacatcttc ttcgtcacta
     2521 aaaaaccgct atgatagcga cagagaatta gcccggagtt tacatggcgt tacgaaataa
     2581 agcgttccat cagttacgac agctttttca gcagcacacg gctcgctggc agcacgagtt
     2641 acctgacctg accaaaccac agtacgcggt gatgcgcgcc attgctgata aacctggtat
     2701 tgaacaggtg gcactgatag aagcagcagt cagcaccaaa gcaacgctag cagaaatgtt
     2761 ggcaagaatg gaaaatcgtg gcttagtcag acgtgaacat gacgtcgctg acaagcggcg
     2821 acgctttgtc tggctgactg ccgaaggaga aaagctactt gctgcggcga tacctattgg
     2881 tgacagcgtg gatgaggaat ttttggggcg tttgagtgga gaagagcagg agctgtttat
     2941 gcagttggtg cgcaagatga tgagcacata gaatgctgat tgccgggtag gacgctgacg
     3001 tgtcttatcc aggcgacaaa aacacaagct tcccaaacaa aagaaaaagg ccagcctcgc
     3061 ttgagactgg cctttctgac agatgcttac ttacttgcgg aacagcgctt cgatattcag
     3121 cccctgcgtt tgcaggattt cgcgcaaacg gcgcaggcct tcaacctgaa tctggcgaac
     3181 acgttcacgg gtgaggccaa tttcacgacc tacatcttcc agtgttgccg cttcataccc
     3241 cagcaaaccg aatcgacgtg ccagtacttc acgctgtttg gcgttcagct cgaacagcca
     3301 tttgacgatg ctctgcttca tatcgtcatc ttgcgtggta tcttccggac cgttctcttt
     3361 ttcatcggcc aggatgtcca gcaacgcttt ttcggaatca ccacccagcg gggtgtctac
     3421 cgaggtaatg cgctcgttaa gacgaagcat acggctgacg tcatcaactg gcttatccag
     3481 ttgctctgcg atctcttccg cacttggttc atggtccagc ttatgggaca actcacgtgc
     3541 ggttcgcagg taaacgttca gctcctttac gatgtgaatc ggcaaacgaa tagtacgggt
     3601 ttggttcata atcgcccgtt caatcgtctg gcgaatccac caggttgcgt atgttgagaa
     3661 gcggaaacca cgttccgggt caaacttctc taccgcgcgg atcagcccca agttgccctc
     3721 ttcgataagg tccagcaacg ccagaccacg attgccataa cggcgggcaa tttttaccac
     3781 cagacgcaag ttactctcga tcatccggcg gcgagaggcg acatctccac gcagtgcgcg
     3841 acgcgcaaaa taaacttctt cttcggccgt taacagtggt gaataaccaa tctcaccaag
     3901 gtaaagctga gtcgcgtcca acacacgctg tgtggctccc tgcgataaca gttcctcttc
     3961 ggccaaatcg ttatcactgg gttcctcttc tactaaggcc ttttcgtcaa aaacctcaac
     4021 tccgttctca tcaaattccg catcttcatt taaatcatga actttcagcg tattctgact
     4081 cataaggtgg ctcctacccg tgatcccttg acggaacatt caagcaaaag cctggttccg
     4141 ccgatttatc gctgcggcaa ataacgcagc gggtttacgg atttcccctt gtaacgaatt
     4201 tcaaaatgca agcgtgttga actggttccg gtgctaccca tggttgctat tttttgcccc
     4261 gccttcactt cttgttgttc ccggaccagc attgtgtcgt tatgggcgta ggcactcagg
     4321 taatcatcat tatgtttgat gataatcaga ttaccgtagc cgcgcagcgc gttaccagca
     4381 taaacaacgc ggccatctgc ggtcgcgata attgcctgtc ctttgctgcc tgcgatatca
     4441 atccccttgt tgcccccctc agaagcgcca aaggtttcga tcactttgcc ctcagtcggc
     4501 cagcgccagg tggagatagg cgtactggtt gatgtactgc tgacagtcgg ctcggttgtg
     4561 cttgctgttg gtaccgttac aggcgctgtg accgtggtcg cagttggctt gttgttcggc
     4621 aacattttgt tagcactctg ttcacccgaa gactcagaat acgtaattgt cggttgcgac
     4681 gcaacagcaa cggtggaatt ttgtgcaggc ttgatcacaa ctccttgctc tgctgcgtcg
     4741 gcctgggtaa tggcatttcc gccagtgatt ggcgtaccgg aagcattacc cacctgcaag
     4801 gtctgaccaa cgttcagcgc gtatggtgcc tgaatattgt tgcgctgagc aaggtcacgg
     4861 aaatcgttgc cagtaatcca ggcgatatag aaaagtgtgt cgcctttttt cacggtatag
     4921 gtactgccgc tataactgcc tttcggaatg ttcccatact gacggttata gacgatgcgt
     4981 ccgttttcca tctgtaccgg ctgctgagct actggctgca ctggctggat ttgcggttgt
     5041 tgagtagcct gaatttgtgg ctgctgtacc ggctgaattt gcggttgctg cgctgtagac
     5101 gtcgtcccca ttttcggcgg cggcgtaatc aacataccag aattagtatt tgcaggcgca
     5161 ttgccattaa cggagctgac cggtgccggt ggatttgaag tgtcagaaca gcctgccagc
     5221 catagcgaaa ccagtgacaa agccgcaatg cggcgaacgg tgaattttgg gcttcccgcg
     5281 ctcatttatc ccccaggaaa aattggttaa taaccagtga cataattacc gtgcaaggca
     5341 ccctactgaa cactggaaaa gatgttcacg atacgctgac ctacggcaaa ataaccagga
     5401 aaaatccagg tatttcctca cgttttaagc cagctcaccc ttcactaaag ggacaaagcg
     5461 cacggcctcc acggtatcga taataaattc gcctccccga cgacgcaccc gtttcaaata
     5521 ctggtgctcc tcccctacgg gtaagacgag aatcccgcct tcgtccagct gcgtcattag
     5581 cgcagttgga atttccggcg gtgccgccgt aacaatgata gcgtcaaacg gcgcacgtgc
     5641 ctgccaacct tgccatccat caccatgacg ggttgaaaca ttatgtaaat caagattttt
     5701 caggcggcga cgtgcctgcc actgcaagcc tttaatccgt tcaaccgagc aaacatgctg
     5761 gacaagatgc gccaggattg ccgtttgata tcccgaacca gtgccaattt ccagcacccg
     5821 cgactgcggc gtcagctcga gtaattcggt cattcgcgcc accatatacg gctgcgaaat
     5881 tgtctgcccc tgacctatcg gcaaggcgat attgtcccag gctttttgtt caaacgcttc
     5941 atcaacgaat ttttcacgcg gcacggcggc aagtgcattc agcacctgct catcctgaat
     6001 accttgcgca cgtaattgat ccagaagtgc ttgtacgcgt ctgcttacca ttgcgtgcca
     6061 actcccacgc tgtttaacca gtctgaaacc acatcttgcg cgctatgcgc agttaaatcc
     6121 acatgcagcg gcgtgatgga gacatagccc tcatctaccg cagcaaaatc ggtccccgga
     6181 ccggcatcac atttaccgcc cggcgggcca atccagtaca gcgtattgcc gcgcggatct
     6241 tgctgcggga tcacctgatc tgccggatgt cgtgtaccgc agcgcgtcac gcgaatacct
     6301 ttgatttgat ccaagggtaa atcctgaacg ttaatattaa gaatacgtcc ggtgcgcagc
     6361 ggctctttac acagtgcgcg caaaattgaa caggttaccg ccgcggcagt gtcgtaatgt
     6421 ttatgcccgt caagcgagac ggccagcgcc ggaaaaccta aatgacggcc ttccatcgcg
     6481 gcggctaccg taccggaata aataacatca tcccccagat tcggcccggc gttaattccg
     6541 gacacaacaa tgtccgggcg cggacgcatc agagcattca cgccaagata gacacaatcg
     6601 gtcggggttc ccatttgcac agcaatatca ccattttcaa aggtaaacgt gcgcagggag
     6661 gattccagtg tcagagaatt tgaagcgccg ctgcggttac gatcgggggc gaccacctga
     6721 acgtcagcaa actcacgcaa ggctttcgcc agcgtttgta taccgggtgc atgtaccccg
     6781 tcataattac tcagcaatat gcgcataatc acctgttgtg ttgataagtt ccctgacaac
     6841 gctggttgca aaactacccg ccggaagcca gaaacggatc tctacggtga cgtcatccca
     6901 ccagttccag cttaattgtt gcggatacag cagcatcgct ctgcgcgcgg cttcaacttt
     6961 ttcgcgcacc agtaaagctt gtaattcagt ttctgcggcg acagctgctt gttcgaatgc
     7021 cagcgcttca cgctgagttc cccattcgcc actgcccggc aatgcggcgg ttatcatcag
     7081 ctctttatcg ttgacgcgac gctgtaattc cgccagttct tcggtggttg ctacaaacca
     7141 gctaccacgt ccggctaatt gtagcgcatc gccgtcaaca acttgattaa cgtctgcttt
     7201 tttgaggcgc tcagcaacaa tctgattaaa caacgcactg cgggctgccg acaaccaaaa
     7261 actccgttta ttgcgatcgc gcaccggagt attggtttgc gcccagcgca gcgcgccctg
     7321 caagttgcta ccgccaatcc caaaacgttg ggcaccgaag tagttcggta caccttttac
     7381 gcaaatatcg atcagacgtt gttcaacgtc atcgcgattg ctcacttcgc gcagaaccag
     7441 ggtaaaggcg ttacctttca gcgcccctaa acgcagcttg cgcttgtgcc gcgcatactc
     7501 cagcacctgg cagccttcca gttgaaaggc gctcagatcg ggcatttcct tgcccggcac
     7561 gcgagcacat aaccactgtt ccgttacagc atgtttgtct ttttgcccag cgaagctgac
     7621 ttcacgggaa tgaattttca ggaatttcgc cagtgcatcc gccacaaaac gggtattgca
     7681 gccgtttttg aggattctaa ccagaatatg ctcaccttca ccatcaggct caaagcccaa
     7741 atcttccacc accacaaagt cttccggatt ggctttcagc agcccggtgc cttgcggttt
     7801 accgtggagg taagtgagat tatcaaactc aatcattttg ttgccttaat gagtagcgcc
     7861 accgcttcac aggcaatccc ttccccacgt ccggtaaatc caagtttttc cgtagtagtg
     7921 gctttcacgt taacatcatc catatggcag ccgagatctt cggcaataaa cacgcgcatt
     7981 tgtggaatgt gcggcaacat cttcggtgcc tgagcgatga tagtgacatc gacgttgcca
     8041 agggtataac ccttcgcctg aatacgacgc caggcttcgc gtagcagctc gcggctgtcg
     8101 gcacctttaa atgccggatc ggtatccggg aacagtttgc cgatatcccc cagcgccgcc
     8161 gcgccaagca atgcatcggt caacgcatga agcgccacgt cgccatcaga atgcgccagc
     8221 aatccttttt cgtaaggaat gcgtacgcca ccaatgataa ttgggccttc accgccaaag
     8281 gcgtgtacgt caaaaccgtg tccaattcgc attatgtatt ctcctgatgg atggttcggg
     8341 tgaggtaaaa ctcggccagt gccaaatctt ccgggcgcgt gactttaatg ttatccgcgc
     8401 ggccttcgac caactgcgga tgaaatccgc aatattccag cgccgaggct tcgtcggtaa
     8461 tggtagcgcc ttcatttaga gcgcgcgtca gacagtcatg taacagctca cgagggaaaa
     8521 attgcggcgt cagcgcgtgc cataagccgt tgcgatcaac ggtatgagca atggcatttt
     8581 tgcccggttc ggcacgtttc atagtatcgc gcactggtgc ggcgaggatc cctcccgtgc
     8641 ggctggtttc gctcaacgcc aacaatcgcg caaggtcatc ctgatgcaaa caaggacgag
     8701 cggcgtcatg caccaatacc cactgcacat cgccagcggc tttcagacct gccagcacgg
     8761 aatcggcacg ctcatcaccg ccatctacaa cggtgatttg cggatgattc gccagaggaa
     8821 gttgtgcaaa acggctatcg ccaggactta tggcaatgac gacacgtgtc acccggggat
     8881 gcgccagcag cgcatgcacc gagtgttcaa gaatggtttg attaccgatt gagagatatt
     8941 gcttaggaca ttccgtttgc attcgacggc caaatccggc cgccggaacc acggcgcaaa
     9001 catccaaatg agtggttgcc atgttaattc ccgggctgat ttatcgattg ttttgccccg
     9061 cagactgtgc gcgcttcgac gcgtcaggca ccagacgata aaaagtttcg cccggcctgg
     9121 tcatgctgag ttcattacgc gcacgctctt cgagcgcctc ctggccgcca ttgagatcgt
     9181 caatttcggc aaaaagttga tcgtttcgcg ctttaagttt cgcgtttgta gcttgcagtg
     9241 ccgccacatc atcattgacg cgggtatagt catgtatacc gttcttaccg aaccacagcg
     9301 aatactgtag ccagaccaga atagccagca acagcagcgt tagtttaccc atcctgcccc
     9361 ctgaaaaact gcatcatcat cccatgcatc cgaagacgac tctacatcct ctgttgggga
     9421 taccgcgaca acgcgggcaa atgtaccaca tttgcccatt gttacgtata cccagggcgt
     9481 gcagaacata atctcattat tagttacggt ttgaattatg aacagaggag acaagaaagt
     9541 acaaattagc ccagtagcca cataaacagt gcgccaaaca taatgcctac tgtcaccagg
     9601 gtgaaaacaa tactgtagcg tagctttccg tccatcaatg aatgcagcgc aatccccacc
     9661 actaccgcga caggcatcag tgccagaaag aaaggccagg tgtagagaaa gaagaacagc
     9721 gtgttagagc cataaatcaa catcggcatc gccagcgcaa ataaccagga gataaaaccg
     9781 accacggcac caggcagtga ccatgtagtt tcttcatcct cagtaaggct gtcgttattt
     9841 gttagtgtaa tgttatggct attacgcata tttgatcctg ttactttgac gaaccgggca
     9901 tggaaacccg gtggtgtctc aggatctgat aatatcgttc tgtctcaaca gatctaataa
     9961 ttgctgtacc aaatttgtta ctaattgttc accattgaga tgaatttctg ccgattcagg
    10021 cgcttcgtaa acggaatcta ttcccgtaaa gttgcgcagt tcaccggcac gcgctttctt
    10081 atataagcct tttggatcgc gggcttcgca aatcgccagc ggcgtatcga caaacacttc
    10141 aataaagcgc ccttctccta cgcgttcgcg aaccatctgg cgttcggcgc ggtgtggcga
    10201 gataaatgcg gtcagcacca ccagtccggc ttcaaccatc aaattcgcca cttcaccgac
    10261 gcgacggata ttctctttac gatcggcatc gctaaaaccg agatcgctgc ataatccgtg
    10321 gcgaacattg tcgccatcca gcagatacgt actgacgctg agtttatgta acgcctcctc
    10381 cagcgccccg gcgaccgttg atttaccgga cccggagagg ccggtaaacc acagcactac
    10441 accacgatga ccgtggtgta gttcgcgttg ttgcacagtg accggatgac tatgccagac
    10501 gacgttttcg tcatgcagcg ccattattta tcccccagca aatcgcgcgc gccccagtgc
    10561 ggaaagtggc gacgaaccag ggcattcaat tccagttcga atgcactgaa ttcagatgac
    10621 gcagctgttg cctggctaac tggctcgtgc accataccgg cacccacggt cacattgctc
    10681 aggcgatcga taaaaatcag cccaccggta accggatttt gctgatagcg atctaacacc
    10741 agtggctcgt caaaagtgag atccaccagg ccgatgccgt tcagcggcag gttttcaact
    10801 tcgcgttggg taaggttatt aatatcaacc tgataacgaa tgccatcaac acgagcacgc
    10861 gtcttcttac cggcaatttt gatgtcataa ctctggcccg gggaaagcgg ctgttccgcc
    10921 atccatacca catccaccga cgcactctgc accgccggta acgcttcgtc tgccgccagc
    10981 agcagatcac cacggctgat atcgatctca tccgtcagca ccagggtgat cgcttctccg
    11041 gcaaagactt cttcgcgatc accatcaaaa gtcacgatcc gcgcgacgtt tgattccaca
    11101 ccagagggca gcacttttac acgttgcccg acttccacgc gaccggatgc cagcattccg
    11161 gcgtagccac gaaaatcgag atttgggcgg ttaacgtact gcaccgggaa gcgcattggc
    11221 tgggcatcca ccactcgctg gatctccacg gtttccagca cttcgagcag tgtcagaccg
    11281 ctgtaccacg gcatactttc actttgcgaa gccacgttgt caccttccag tgcggagagt
    11341 ggcacaaagc ggatatccag attacccggc agctgcccgg caaaggtcag ataatcttca
    11401 cgaatacggg tgaacgtctc ttcactgtaa tccaccagat ccattttgtt gatcgccacg
    11461 accagatgtt tgatccccaa cagtgtggag ataaaactgt gacgacgggt ttgatcgagc
    11521 acgcctttac gggcatcaat cagtaagatc gccagttcac atgtcgatgc gccagtcgcc
    11581 atattgcgag tgtactgctc gtgccctggg gtgtcggcga taataaattt acgcttctcg
    11641 gtagagaaat agcggtaggc cacatcaata gtgatgccct gttcgcgctc agcttgcagg
    11701 ccgtccacca gcagagccag atccagcttt tcgccctggg ttccgtgacg cttactgtcg
    11761 ttatgcagcg atgagagctg atcttcgtag atttggcggg tatcgtgcag cagacgacca
    11821 atcagggtac ttttgccgtc atcgacgcta ccacaggtca gaaaacgcag caggctttta
    11881 tgttgttgcg caatcatcca ggcttcgacg ccgccttcat tggcgatttg ttgtgcaagt
    11941 acggtgttca tcttaaaaat acccctgacg ttttttcagc tccatggatc ccgcctggtc
    12001 gcggtcaatc acgcgaccct gacgttcact ggtggtggaa accagcatct cttcgatgat
    12061 ttccggcagt gtttgtgcat ttgactccac cgcaccggtc agcggccagc agcccagcgt
    12121 acggaaacgc accatccgtt ttttgatcac ttcgcccggt tgcaggtcga tacggttgtc
    12181 atcaatcatc atcaacatac cgtcgcgttc cagaaccgga cgttccgcag cgagatacag
    12241 cggcacaatg tcgatatttt ccagccagat gtattgccag atatcctgct cggtccagtt
    12301 agagagcggg aagacgcgga tgctttcgcc tttgttaatt tgcccgttgt agttgtgcca
    12361 cagctccggg cgctgatttt tcggatccca gcgatggaag cggtcacgga aagagtagat
    12421 tcgctcttta gcgcgggatt tctcttcgtc acggcgcgca ccaccgaagg cggcatcaaa
    12481 accgtatttg ttcagcgcct gtttcaggcc ttcagttttc ataatatcgg tatgtttcgc
    12541 gctgccgtgc acgaatggat taatccccat cgccacgcct tccgggtttt tatgcaccag
    12601 cagctcgcag ccgtaggctt tcgccgtacg atcgcggaac tcatacatct cgcggaattt
    12661 ccagccggta tcgacatgca gcaacgggaa aggcagcgta cctggataaa acgccttgcg
    12721 cgccagatgc agcatgacgc tggaatcttt accgatagag tagagcatca ccggatttga
    12781 gaattctgcc gccacctcgc gaataatgtg gatgctttcc gcctccagtt gccgcaggtg
    12841 agtaagtcgt ttttgagcca taaccgttcc tttgcaatac cgctattttc ttgccatcag
    12901 atgtttcgac tatagggagc gtaagagaac gaatgaaatt accaattaga atgagtagtt
    12961 ccttaacgga ataacgattt ggcaaagcta atatcaaaaa gtgcttaagg caccggattt
    13021 cgggcgttta ggaagatttg aaattgtttt agcgcagcgg cagtttcata ctatggcggt
    13081 aaaaaaattt gcatggtatt taaggactca ctatgttttc cgcattgcgc caccgtaccg
    13141 ctgccctggc gctcggcgta tgctttattc tccccgtaca cgcctcgtca cctaaacctg
    13201 gcgattttgc taatacccag gcacgacata ttgctacttt ctttccggga cgcatgaccg
    13261 gaactccagc agaaatgtta tctgccgatt atattcgcca acagtttcag caaatgggtt
    13321 atcgcagtga tattcggaca tttaatagtc ggtatattta taccgcccgc gataatcgca
    13381 agaactggca taacgtgaca ggaagtacgg tgattgccgc tcatgaaggc aaagcgccgc
    13441 agcagatcat cattatggcg catctggata cttacgcccc gctgagcgat gctgacgcag
    13501 atgccaatct cggcggactg acgttacaag gaatagatga taacgccgca ggtttaggtg
    13561 tcatgctgga actggcagaa cgcctgaaaa atacgcctac cgagtatggt attcgatttg
    13621 tggcgaccag cggcgaagag gaaggaaaat taggcgctga gaatttactc aagcggatga
    13681 gtgacaccga aaagaaaaat acgctgctgg tgattaatct cgataactta attgttggcg
    13741 ataaattgta tttcaacagc ggtgtaaaaa cccctgaagc agtaaggaaa ttaacgcgcg
    13801 acagggcgct ggcaattgca cgcagtcacg gaatagccgc aacgaccaat ccgggtttga
    13861 ataaaaatta tccgaaaggc actgggggtt gtaatgacgc agaaatattc gacaaagcgg
    13921 gcattgctgt actttcggtg gaagcgacta actggaatct tggaaataag gatggttatc
    13981 agcaacgcgc aaaaacacct gccttcccgg cgggaaatag ctggcatgac gtaagactgg
    14041 ataatcaaca acatattgat aaggcacttc ctggaagaat agaacgtcgc tgccgtgacg
    14101 ttatgcggat aatgctaccg ttggtgaagg agctggcgaa ggcgtcttga tgggtttgaa
    14161 aatgggagct gggagttcta ccgcaggggc ggggaattct aagtgatacc catcatcgca
    14221 tccagtgcgt ccggtttact tccgggaaaa ccgcaccgta ttgcgccggt ttatctccgc
    14281 tggagcgggg aacacctctg gcgaatagaa agcaagccag gttttgacca gtcgatcgta
    14341 catggcattg gccgttgcca ttgttggcag gccaaaaaac agcccctgag cgttacccgc
    14401 agccatcaac ctgtgcgcca ggataagcgc cgcttctgtt ttacctgcgc cggtcacatc
    14461 ttccagaata aacaactgtg gtcctggctg attgatatcc agatccagca ccttttgctg
    14521 taacggtgtc gtattctcaa taaaaggaaa cagcgtatta attccggtga aaggcgcggt
    14581 ttctgcttgt ggcggaaaga cggttaatgc gttttgagcc tgaactaaag ccttttgcca
    14641 gtaatcttca atatccattg cctgtgcgac gcgtggaaaa aatcgcgttg acgaccctgt
    14701 ccagtctgca agtacgacgg ttgcagagat ataccaggaa agttgtttta taagttcaac
    14761 gccctcgtca tcatcccaga atgtggggat ctctattcgc ggaaacagtg gcttgatttc
    14821 aaggagaaaa tctcgtgctg cagctttgtc ttcaggcaga aaattatcca attcatccat
    14881 acgatcaggg ggccgaccat gatgccctgt cgtcacgggc atccacattt ctatcactcg
    14941 cctaagtttg cgcggagaga gcgaagatga aggaagtaac tcctgacatt cacttaaata
    15001 atgattccac aaccagtagc ccagcgttga atgagagatt ttttctggca ccttcctgaa
    15061 tcttgaattc agggggcagg taaagttgtt gaaaagagcg ggcaaatttt ccaatatcgt
    15121 gccagcacag caaccaggcg aaaaatcgag ctgcctgttc cttgtcagaa atccctaatt
    15181 gacgaaaata atcagccagc ccgaagcaat ttcttttaac cattaaatag cccattgcgg
    15241 ccacatccag caaatgccag caaagaaggt gatagtcgtc gataccctct ttctcgccac
    15301 gtcggttttt tccccagaaa tcaaagaaag tcacaatatt tttatccttc agtaaactta
    15361 aaggatattt acgcatattt
//
LOCUS       DAZOYF010000092        15300 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZOYF010000092 DAZOYF010000000
VERSION     DAZOYF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.092"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACHUOF_002666"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACHUOF_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR0611790.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="ACHUOF_002667"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="ACHUOF_002667"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR0611791.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="ACHUOF_002668"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="ACHUOF_002668"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR0611792.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="ACHUOF_002669"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="ACHUOF_002669"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR0611793.1"
                     /translation="MKTLLIIDANLGQARAYMVKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="ACHUOF_002670"
     CDS             5718..6659
                     /locus_tag="ACHUOF_002670"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR0611794.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="ACHUOF_002671"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="ACHUOF_002671"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR0611795.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="ACHUOF_002672"
     CDS             7679..8929
                     /locus_tag="ACHUOF_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0611796.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /locus_tag="ACHUOF_002673"
                     /pseudo
     CDS             complement(9000..9694)
                     /locus_tag="ACHUOF_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="ACHUOF_002674"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="ACHUOF_002674"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR0611797.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="ACHUOF_002675"
     CDS             10866..12116
                     /locus_tag="ACHUOF_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0611798.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="ACHUOF_002676"
     CDS             complement(12172..13260)
                     /locus_tag="ACHUOF_002676"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR0611799.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACHUOF_002677"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACHUOF_002677"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR0611800.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="ACHUOF_002678"
     CDS             complement(14194..15243)
                     /locus_tag="ACHUOF_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR0611801.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
BASE COUNT         3769 a         3692 c         4091 g         3748 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggtga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttatttat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
//
LOCUS       DAZOYF010000093        15156 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZOYF010000093 DAZOYF010000000
VERSION     DAZOYF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.093"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            142..885
                     /locus_tag="ACHUOF_002679"
     CDS             142..885
                     /locus_tag="ACHUOF_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0611802.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(942..2375)
                     /gene="bglA"
                     /locus_tag="ACHUOF_002680"
     CDS             complement(942..2375)
                     /gene="bglA"
                     /locus_tag="ACHUOF_002680"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR0611803.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            2420..2730
                     /gene="yqfB"
                     /locus_tag="ACHUOF_002681"
                     /pseudo
     CDS             2420..2730
                     /gene="yqfB"
                     /locus_tag="ACHUOF_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
     gene            2894..3553
                     /locus_tag="ACHUOF_002682"
     CDS             2894..3553
                     /locus_tag="ACHUOF_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR0611804.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(3749..4729)
                     /gene="ygfZ"
                     /locus_tag="ACHUOF_002683"
     CDS             complement(3749..4729)
                     /gene="ygfZ"
                     /locus_tag="ACHUOF_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR0611805.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            4972..5238
                     /gene="sdhE"
                     /locus_tag="ACHUOF_002684"
     CDS             4972..5238
                     /gene="sdhE"
                     /locus_tag="ACHUOF_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR0611806.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            5219..5626
                     /locus_tag="ACHUOF_002685"
     CDS             5219..5626
                     /locus_tag="ACHUOF_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR0611807.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(5666..6187)
                     /gene="fldB"
                     /locus_tag="ACHUOF_002686"
     CDS             complement(5666..6187)
                     /gene="fldB"
                     /locus_tag="ACHUOF_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR0611808.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            6299..7195
                     /gene="xerD"
                     /locus_tag="ACHUOF_002687"
     CDS             6299..7195
                     /gene="xerD"
                     /locus_tag="ACHUOF_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR0611809.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            7220..7930
                     /gene="dsbC"
                     /locus_tag="ACHUOF_002688"
     CDS             7220..7930
                     /gene="dsbC"
                     /locus_tag="ACHUOF_002688"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR0611810.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            7936..9669
                     /gene="recJ"
                     /locus_tag="ACHUOF_002689"
     CDS             7936..9669
                     /gene="recJ"
                     /locus_tag="ACHUOF_002689"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR0611811.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            9760..10858
                     /gene="prfB"
                     /locus_tag="ACHUOF_002690"
     CDS             join(9760..9834,9836..10858)
                     /gene="prfB"
                     /locus_tag="ACHUOF_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR0611812.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            10868..12385
                     /gene="lysS"
                     /locus_tag="ACHUOF_002691"
     CDS             10868..12385
                     /gene="lysS"
                     /locus_tag="ACHUOF_002691"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR0611813.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(12428..12976)
                     /gene="idi"
                     /locus_tag="ACHUOF_002692"
     CDS             complement(12428..12976)
                     /gene="idi"
                     /locus_tag="ACHUOF_002692"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR0611814.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            13031..13102
                     /locus_tag="ACHUOF_002693"
     CDS             13031..13102
                     /locus_tag="ACHUOF_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611815.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(13099..13224)
                     /gene="yqfG"
                     /locus_tag="ACHUOF_002694"
     CDS             complement(13099..13224)
                     /gene="yqfG"
                     /locus_tag="ACHUOF_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR0611816.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(13226..14674)
                     /gene="uacT"
                     /locus_tag="ACHUOF_002695"
     CDS             complement(13226..14674)
                     /gene="uacT"
                     /locus_tag="ACHUOF_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR0611817.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         3802 a         3751 c         3992 g         3611 t
ORIGIN      
        1 gcactggcgc gaatatttac cctcaccccg accctctccc tgaaagggcg agggggaaaa
       61 gcgtgccaac attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat
      121 taatttttct cggaaaaaca aatggctata gcacttgtga ccggtggcag tcgcggcatc
      181 gggcgggcaa ctgcattact gttggcgcaa gaagggtata cggtggcggt taattatcag
      241 caaaacctcc acgcggcgca ggaagtgatg aacttaataa tgcaagccgg tggcaaagca
      301 ttcgtgctcc aggcggatat cagcgacgaa aaccaggtcg ttgcgatgtt tacagcaatc
      361 gatcagcacg atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag
      421 tgcaccgttg aaaaccttac cgcagagcga atcaaccgag tactttccac caacgtgacg
      481 ggatattttc tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt
      541 ggcggcgcta tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat
      601 gttgattacg cggcatcgaa aggggcgatt gattcgttaa ccaccggact atcgctggaa
      661 gtcgccgcgc aggggatccg cgttaactgc gtgcggccag ggtttattta taccgaaatg
      721 cacgccagcg gcggcgagcc tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag
      781 cgtggtggac aggcagaaga ggtcgcgcag gccattgtct ggctactaag tgataaagcc
      841 tcttacgtca cgggaagttt tatcgatttg gcgggcggga aataaaacag ggaagttgtc
      901 tgaccggatg caacaagtat tgcatccggt acttcatcga cttaaagctt ctcgccgttg
      961 ctggcaatca cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca
     1021 gtaccgtcgt catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggta
     1081 gtgaatgaca cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacaccatcg
     1141 taagtcactg cttttttcat ctcttcaata tgggcgcgca ggtagtcaat gcggtagtcg
     1201 tcgttgatgc tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata
     1261 aacagcggcc tctgataacg ctcatacagt tcgcaaaggg catagcgcag acctactgga
     1321 tcaatctgcc agccccagtc agacgcttta acatacgggt tcggtacgct gccttcaaaa
     1381 ccagagatcg catcgccggt gccgccttcg gccttcactg cgttggtcat gtaatagctg
     1441 aaaccaagat aatcgcaggt gccttcgcgc agtacatcga gatcgccgtc ttccattttg
     1501 atattaaatc cgcgacgttc ccactcgttc aatacatagg acgggtaata gccgcatagc
     1561 tgcacatcgg taaagacgta gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc
     1621 gggttacatg agtagggata gagcggcacc atcgccagca tacagccgac tttcatctcc
     1681 gggttaatgc gacgcgcggc tttcactgcc agggcgctgg cgacaaactg gtgatgcagc
     1741 acctggtaca tcgtctcttc cgggttgtca tgctcggtat acaccacgcc ggagcagcag
     1801 taaccaaaca gcggcgcacg ccagttacgc tggttgttta tttcgttgaa ggtcatccag
     1861 tatttgactt tatgcttgta gcgttcgaat accacttccg cgaaacggac aaagaaatca
     1921 acgaatttac ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag
     1981 tgggagaggg ttatcaccgg ttcgatgttg tatttcagca gttcatcgaa cagagagtcg
     2041 taaaacttca gcccttcttc gtttggctga gcttcatcac cttttggaaa aatacgcgtc
     2101 caggcgatgg aagtccgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc
     2161 ttgtagtgac cataaaaatc aacggcttca tggtttggat agtattttcc tggcaagact
     2221 tctttggtga tttcgcgcgg cacgccgtgt gcgccacagg tcagaacgtc acaaatgctc
     2281 ggtcctttgc cgcctttgtt ccagccgcct tcgacctgat gagcggcaac ggcgccaccc
     2341 cataagaaat ctttcggtaa ggtgagtttt ttcactatca taggctccag atcagtcaat
     2401 tcttacagga aatagtacca tgcagccaaa cgacatcact tttttcaacg tttccaggat
     2461 gacattctgg ctgggcgtaa aaccatcacc atccgcgacg agtctgaatc gcacttcaaa
     2521 acgggtgatg tgcttcgtgt cggacgtttt gaagatgacg gttatttttg cacgattgaa
     2581 gtcaccgcaa cctcaaccgt aacgctggat acactgacgg aaaaacatgc agagcaggaa
     2641 aatatgaccc tgcctgaact gaaaaaggtc attgctgaca tctatcccgg tcagacacaa
     2701 ttttatatga ttgaatttaa atgtctttaa attgaactca cgcctgttag ttgaaatttc
     2761 gcatttaatt tcatgatttt taggagatta gtctcaaatc agaatttatt ttagctaaca
     2821 ggtgttcact ggaactattc tcagttacgc tggagaggta tacatgtgcg tgtacgtttc
     2881 tctggagtaa gttatggttc agaagcccct cattaagcag ggatattcgc tggcagagga
     2941 aattgccaac agcgtcagtc acggcattgg gctggtgttt ggtatcgttg ggctggtgtt
     3001 gctactggtt caggcggtgg atctcaatgc cagcgccaca gcgataacca gttacagcct
     3061 ctatggcggc agtatgatcc tgctgttcct cgcttcgacg ctctatcacg ccattcctca
     3121 tcaacgggca aaaatgtggc tgaagaaatt tgaccattgc gctatttacc tgttgattgc
     3181 cggaacctac acgccgtttt tgctggtggg gctggattct ccgttagcgc gcgggttgat
     3241 gattgttatc tggagcctag cattgctggg gattctgttc aaactgacca tcgcgcaccg
     3301 attcaaaatt ttatctctgg tgacttatct ggcgatgggc tggctgtcgc tggtggtaat
     3361 ttatgaaatg gcagttaagc tcgcggcggg cagcgttacc ttactggcgg taggcggcgt
     3421 ggtttattcg ctcggggtga ttttctacgt ctgcaaacgc attccataca accatgccat
     3481 ctggcacggc ttcgtgctcg gcggtagtgt gtgccacttt ctggcgatct atttgtatat
     3541 tgggcaggcg taatgggtat gcctttgtag gcctgataag acgcgtcagc gtcgcatcag
     3601 gcatcggagc gcaaccgccg gatgcggcgt gaacgcctta tccgatctac gggcctcgtc
     3661 cctgttgtga taaaggcatc aaatcccaga catgataaga cgcgtcagcg tcgcatcagg
     3721 cgataaatgc ctgatgcgat acagagattt actcttcgag cgaatacggc agcggctcga
     3781 tatgcagtgt attcgcatcg tcgcgtacgc ggaagatgct gtccggttcc atatcgttat
     3841 tcatgacgac ctgtaccacg acctgaccat cttccagttt taccgcagcc agcaccgtac
     3901 cggtacgacg ccagttctcg cccattttca gctctaagtc ttcaccagct tccggcagtc
     3961 ggctggcact acctgccagc aaccagagcg cgcgtttgtt ggcaccacgg aattttgctc
     4021 gcgccaccat ctcttgtccg gtataacagc ctttcttaaa gctgataccg cccagcgcct
     4081 ggagattggt cgcctgtggg ataaactgcc cgctgttggc ggcatcaatc accgggaaac
     4141 cagcttcaat gtttaatgcc agccactgtt ggctattgtt cagttccgct tcaccgcgca
     4201 gtttatcggt cagcatatta gcagtagctt catcggttac gatcaggaaa cgttctgccg
     4261 ggtgttcaaa ccatagcaaa gtggtcgcgc cttctttgac tacctgtttt tctttcgaag
     4321 gcagttcgct aaagagattt gccagcgcgg cgcgcgcctg aaaaccggca acaccaagca
     4381 gcacacgctc gtcgtctggc gcgatggtca ctttagagaa taccgcatat tttttcagtt
     4441 cagtcagctg cggttcacgc acgctgcgcc gttcaatcca tgcaaagcca tcgccgtcgc
     4501 ggaacagacg taaattgctc cacattttac ctttggcgtc gcaatgggcg gcgagcaggt
     4561 gctgatcttc tgccatctgg ctgacatctg ctgtcacctg accctgcata tatttttcgc
     4621 tgtccgcgcc agtaatggtg gcaagcgccc agtcatcaag cgtcatcagc gtcagcggta
     4681 aacgggcaga agccgtaggc tgacgtggag gaaaaggtgt aaaagccata atagagtcct
     4741 gagttgctta acggcgtatt aatgactaat ggtaaaagag ctatgaggca atgcaagtgc
     4801 tttcactacg cgatctgtgc cttctgcgag gcgactgcca aaaaaatgaa gcccttgaat
     4861 tgttgtgaat taatttcgcg atcgcgatgg tgtttactga tattcccgta aaagaagtcg
     4921 aaaaacacgt tacaatagcc gggtactgga ttttcgtgag acaggaagaa gatggacatt
     4981 aacaacaaag cccgaattca ttgggcatgc cgccgtggta tgcgcgaact cgatatttca
     5041 atcatgtcgt ttttcgaaca tgagtacgac agcttaagcg atgacgaaaa acgcatcttt
     5101 attcgtctgc tggaatgtga cgatccggac ctgtttaact ggctgatgaa tcacggtaaa
     5161 ccggccgatg cagaactgga aataatggtc cgactcatcc agacacggaa ccgggaacgt
     5221 ggtcctgtgg caatctgatt tgcgcgtctc ctggcgcgca cagtggcttt ccttgctgat
     5281 tcatgggctg gttgccgctg ttattttact catgccctgg ccgctcagtt acaccccgtt
     5341 atggatggtg ttactttcgc tggtggtatt tgattgcgtt cgcagccagc ggtgtattaa
     5401 tgctcgccag ggggaaattc gcttgttgat ggacgggcgt ttgcgttggc aagggcagga
     5461 gtggagcatc gtcaaagcac cgtggatgat taagagcggc atgatgctgc gtttacgttc
     5521 tgatggcggt aaacggcaac atttatggct ggcagccgac agcatggacg aagccgaatg
     5581 gcgggattta cggcggattt tgttgcaaca agagacgcaa agataaagat gacggcaaag
     5641 catatgcgct aagccgtcag tgagatcagg cgtaatgctc tgccatttcg ttgaggattt
     5701 gctcgcacca gctctgaata cgctcgtcgc taaggtcata ctggttagtt tcatccagcg
     5761 ccagacccac gaacagttgc ccgtcagcaa tcaccggttt cgggctggta aattcatatc
     5821 cttccgttgg ccagtagccg acgaacttca cgcctttggt cgagagtttg tcatgcagca
     5881 taccgagcgc atcgaggaac cactcgccgt atcccagttg atcgccaagc ccatacagcg
     5941 caataatttt accttcaagg ttcaggtcgt cgagctgatc ccagacggct tcccagtctt
     6001 cctggatttc accaaaatcc caggtcggga tacccagaat gagcacatcg tactgctcca
     6061 ttaatttcgg ggagtcgtcc ttgaggttat gtaaggtcac cagttctggg ccgataatat
     6121 cgcggatttt ttctgccgcc atttcggtgt aacaggtgct ggaaccgtaa aaaagaccca
     6181 tattcataac gtaagcttct cgattctgct gtggcttttg cagctagtgt atcagaatcg
     6241 ctttagtcac ggcacaataa ggcataatgg atcaaatgag tgaccataag gggcgcaagt
     6301 gaaacaggag ctggcacgca tcgagcagtt tcttgatgct ctgtggctgg aaaaaaatct
     6361 ggctgaaaat acgttgaacg cttaccgtcg cgatctgtca atgatggtgg agtggttgca
     6421 tcaccgcggg ttgacgctgg cgacggcgca aagtgacgat ttgcaggcat tactggcaga
     6481 acggctggag ggcgggtata aagccaccag ctcagcgcgt ttgctgagtg cagtgcgccg
     6541 attgttccag tatctttatc gcgaaaagtt tcgtgaagac gatcccagtg cgcatctcgc
     6601 ttcaccgaaa ttgccccagc gtttgccaaa agatttaagc gaagcgcagg tcgaacgttt
     6661 attacaggcg ccattaattg atcagccact ggagctacgc gataaagcca tgcttgaagt
     6721 gttgtatgct accgggctgc gtgtctctga actggtcggg ctgacaatga gtgatatcag
     6781 cctgcgtcag ggcgtagtgc gggtcattgg taaaggcaac aaagagcgcc tggtgccgtt
     6841 aggggaagag gcggtttact ggctggaaac ctatctggaa catgggcgtc cgtggctgct
     6901 gaatggtgtg tcaattgacg tgttgtttcc cagccagcgt gcgcagcaga tgacgcgaca
     6961 gaccttctgg caccgcatta aacattatgc agtgctggcg ggtatcgaca gcgaaaagct
     7021 gtcaccgcat gtgttgcgtc acgcttttgc cactcattta ttaaatcatg gtgcggattt
     7081 acgcgtggtg cagatgctac tgggccacag cgatctttcc accacgcaaa tttatacgca
     7141 tgtcgctacc gagcgtctgc ggcaacttca tcaacagcat cacccgcggg cgtgatgtct
     7201 gaaaagaacg ggaagattta tgaagaaagg ttttatgttg tttactttgt tagcggcgtt
     7261 ttcaggcttt gctcaggctg atgacgcggc aattcaacaa acgttagcca aaatgggcat
     7321 caaaagcagc gatattcagc ccgcgcccgt agctggcatg aagacagttc tgactaacag
     7381 cggtgtgttg tacatcaccg atgatggtaa acatatcatt caggggccaa tgtatgacgt
     7441 tagtggcacg gctccggtca atgtcaccaa taagatgctg ttaaagcagt tgaatgcgct
     7501 tgaaaaagag atgatcgttt ataaagcgcc gcaggaaaaa cacgtcatca ccgtgtttac
     7561 tgatattacc tgtggttact gccacaaact gcatgagcaa atggcagact ataacgcgct
     7621 ggggatcacc gtgcgttatc ttgctttccc gcgccagggg ctggacagcg atgcagagaa
     7681 agaaatgaaa gctatctggt gtgcgaaaga taaaaacaaa gcgtttgatg atgtgatggc
     7741 aggtaaaagc gtcgcaccag ccagctgcga cgtggatatt gccgaccatt acgcacttgg
     7801 cgtccagctt ggcgttagcg gtactccggc agttgtgctg agcaatggca cacttgttcc
     7861 gggttaccag ccgccgaaag acatgaaaga attcctcgac gaacaccaaa aaatgaccag
     7921 cggtaaataa ttcgcgtgaa acaacagata caacttcgtc gccgtgaagt cgatgaaacg
     7981 gcagacttgc ccgctgaatt gcctcccttg ctgcgccgtt tatacgccag ccggggagta
     8041 cgcagtgcgc aagaactgga acgcagtgtt aaaggtatgt tgccctggca gcaactaagc
     8101 ggtgtcgaaa aggccgttga gatcctttac aacgcttttc gcgaaggaac gcggattatt
     8161 gtggtcggtg atttcgacgc cgacggcgcg accagcacgg ctctaagcgt gctggcgatg
     8221 cgctcgcttg gttgcagcaa tatcgactac ctggtaccaa accgtttcga agacggttac
     8281 ggcttaagcc cggaagtggt cgatcaggcc catgcccgtg gcgcgcagtt aattgtcacg
     8341 gtggataacg gtatttcctc ccatgcggga gttgagcacg ctcgctcgtt gggcatcccg
     8401 gttattgtta ccgatcacca tttgccgggc gacacattac ccgcagcgga agcgatcatt
     8461 aaccctaact tgcgcgactg taatttcccg tcgaaatcac tggcaggcgt gggtgtggcg
     8521 ttttatctga tgctggcgct gcgcaccttt ttgcgcgatc agggctggtt tgatgagcgc
     8581 ggcatcgcaa ttcctaacct ggcagaactg ctggatctgg tagcactggg gacagtagcg
     8641 gacgtcgtgc cgctggacgc taataatcgc attctgacct ggcaggggat gagtcgcatc
     8701 cgtgccggaa agtgccgtcc agggattaaa gcgctgctgg aagtggcaaa tcgcgatgca
     8761 caaaaactag ccgccagcga tttaggtttt gcgctgggac cgcgtctcaa tgctgccggg
     8821 cgactggatg atatgtccgt tggtgtggcg ctgttgctgt gcgacaacat cggcgaagcg
     8881 cgcgtgctgg caaatgaact cgatgcgcta aaccagacgc gaaaagagat cgaacaagga
     8941 atgcaggttg aagccctgac cctgtgcgag aaactggagc gcagccgtga cacgctaccc
     9001 ggcgggctgg caatgtatca ccccgaatgg catcagggcg ttgtcggtat tctggcttcg
     9061 cgtatcaaag agcgttttca ccgtccggtt atcgcctttg cgccagcagg tgacggtacg
     9121 ctgaaaggtt ccggtcgctc cattcagggg ctgcatatgc gtgatgcgct ggagcgatta
     9181 gacacactct accccggcat gatgctcaag tttggcggtc atgcgatggc ggcgggtttg
     9241 tcgctggaag aggataaatt cgaactcttt caacaacggt ttggcgaact ggttactgag
     9301 tggctggccc cttcgctatt gcaaggcgaa gtggtgtcag acggtccgtt aagcccggcc
     9361 gaaatgacca tggaagtggc gcagctgctg cgcgatgctg gcccgtgggg gcagatgttc
     9421 ccggagccgc tgtttgacgg tcatttccgt ctgctgcaac agcggctggt gggcgaacgt
     9481 catttgaagg tgatggtcga accggtcggc ggcggtccac tgctggatgg tattgctttt
     9541 aatgtcgata ccgctctctg gccggataac ggcgtgcgcg aagtgcaact ggcttataag
     9601 ctcgatatca acgagtttcg cggcaaccgc agcctgcaaa ttatcatcga caatatctgg
     9661 ccaatttagc gtcatcttct ctataaaaaa gagcgtggat tgggtacaat cccgctctta
     9721 tcaccgcatt ttgactagct caataaaaga aatcagacca tgtttgaaat taatccggta
     9781 aataatcgca ttcaggacct cacggaacgc tccgacgttc ttagggggta tctttgacta
     9841 cgacgccaag aaagagcgtc tggaagaagt aaacgccgag ctggaacagc cggatgtctg
     9901 gaacgaaccc gaacgcgcac aggcgctggg taaagagcgt tcctccctcg aagccgttgt
     9961 cgacaccctc gaccaaatga aacaggggct ggaagatgtt tctggtctgc tggaactggc
    10021 tgtagaagct gacgacgaag aaacctttaa cgaagccgtt gctgaactcg acgccctgga
    10081 agaaaaactg gcgcagcttg agttccgccg tatgttctct ggcgaatatg acagcgccga
    10141 ctgttacctc gatattcagg cggggtctgg cggtacggaa gcacaggact gggcgagcat
    10201 gcttgagcgt atgtatctgc gctgggcaga atcgcgtggt ttcaaaactg aaatcatcga
    10261 agagtcggaa ggtgaagtgg cgggtattaa atccgtgacg atcaaaatct ccggcgatta
    10321 cgcttacggc tggctgcgta cagaaactgg cgttcaccgc ctggtgcgta aaagcccgtt
    10381 tgactccggc ggtcgtcgcc acacgtcgtt cagctctgcg tttgtttatc cggaagttga
    10441 tgatgatatt gatatcgaaa tcaacccggc ggatctgcgc attgacgttt atcgcgcgtc
    10501 cggcgcgggc ggtcagcacg ttaaccgtac cgaatctgcg gtgcgtatta cccacatccc
    10561 gaccgggatc gtgacccagt gccagaacga ccgttcccag cacaagaaca aagatcaggc
    10621 catgaagcag atgaaagcga agctttatga actggagatg cagaagaaaa atgccgagaa
    10681 acaggcgatg gaagataaca aatctgacat cggctggggc agccagattc gttcttatgt
    10741 ccttgatgac tcccgcatta aagatctgcg caccggggta gaaacccgca acacgcaggc
    10801 cgtgctggac ggcagcctgg atcaatttat cgaagcaagt ttgaaagcag ggttatgagg
    10861 aaccaacatg tctgaacaac acgcacaggg cgctgacgcg gtagtcgatc ttaacaatga
    10921 actgaaaacg cgtcgtgaga agctggcgaa cctgcgtgag cagggaattg ccttcccgaa
    10981 cgatttccgt cgcgatcata cctctgacca attgcacgca gaattcgacg gtaaagagaa
    11041 cgaagaactg gaagcgctga acatcgaagt cgccgttgct ggccgcatga tgacccgccg
    11101 tattatgggt aaagcgtctt tcgttaccct gcaggatgtg ggtggccgca ttcagctgta
    11161 cgttgcccgt gacgatctgc cggaaggcgt ttacaacgag cagttcaaaa aatgggacct
    11221 cggcgacatc ctcggcgcga aaggtaaact gttcaaaacc aaaaccggcg aactgtctat
    11281 ccactgtacc gagctgcgtc tgctgaccaa agcactgcgt ccgctgccgg ataaattcca
    11341 cggcttgcag gatcaggaag cgcgctatcg tcagcgttat ctggatctca tctctaacga
    11401 tgaatcccgt aacaccttta aagtacgctc gcagatcctc tctggtattc gccagttcat
    11461 ggtgaaccgc ggctttatgg aagttgaaac gccgatgatg caggtgatcc caggcggtgc
    11521 cgctgcgcgt ccgtttatca cccaccataa cgcgctggat ctcgacatgt acctgcgtat
    11581 cgcgccggaa ctgtacctca agcgtctggt ggtcggtggc ttcgagcgtg tattcgaaat
    11641 caaccgtaac ttccgtaacg aaggtatttc cgtacgtcat aacccagagt tcaccatgat
    11701 ggaactctac atggcttacg cagattacaa agatctgatc gagctgaccg aatcgctgtt
    11761 ccgtactctg gcacaggata ttctcggtaa gacggaagtg acctacggcg acgtgacgct
    11821 ggacttcggt aaaccgttcg aaaaactgac catgcgtgaa gcgatcaaga aatatcgccc
    11881 ggaaaccgac atggcggatc tggataactt cgactctgcg aaagcgattg ctgaatctat
    11941 cggtatccac gttgagaaga gctggggtct gggccgtatc gttaccgaga tcttcgaaga
    12001 agtggcagaa gcacatctga ttcagccgac cttcattact gaatatccgg cagaagtttc
    12061 tccgctggcg cgtcgtaacg acattaaccc ggaaatcaca gaccgctttg agttcttcat
    12121 cggtggtcgt gaaatcggta acggctttag cgagctgaat gacgcggaag atcaggcgca
    12181 acgcttcctg gatcaggttg ccgcgaaaga cgcaggtgac gacgaagcga tgttctacga
    12241 tgaagattac gttaccgcac tggaacatgg cttaccgccg acagcaggtc tggggattgg
    12301 catcgaccgt atggtaatgc tgttcaccaa cagccatacc atccgcgacg ttattctgtt
    12361 cccggcgatg cgtccggtga aataagcatt acgttatgct cacaaccccg gcaaatgtcg
    12421 gggtttttta tttaagctgg gtaaatgcag ataatcgtat tctggcttcg cgatttgtcg
    12481 cctgcatcac catccacgga ctgaacgccc acggcgtggc atcaataccg cgtaatacat
    12541 cagctaaatc acaccattga taatccatca cttcatcatc gttgatctgt aacgcactgg
    12601 tggtgcgtgc ggcaaatacc ggacacactt cattttccac aatgcctctc ggatcggtgg
    12661 cgcggtagcg aaagtcagga tagatagatt caggaggcgt aatttccacg ccaagctcat
    12721 aacggcaacg gcggatcacc gcgtcttcgc tgctttctcc cagttgtggg tgcccacaaa
    12781 ccgagttagt ccacacgcca ggccacgctt ttttgctcag tgcgcggcgg gtaactaata
    12841 attgcccctt ggcattaaac agccaactgg agaacgcgag atgtaagagg gtgtctgccg
    12901 tgtgtgcggc atacttttcc agcgtacccg tgggaactcc ctgtgcattc aataaaatga
    12961 cgtgttccgt ttgcataatt tctcacatgt aattctgatc actcatcatt atagataatt
    13021 gaagagtgaa atgattaacc aagagagcgt ttatcgacaa ccgcccgttt tgagcggatg
    13081 ccgacaggta aaaaccattt aaaaaagtag catcatatat ttgaagcgac tttcaatggc
    13141 cattgcaaca cagtcagaaa tgacaggaat agagagagta aaaagcaaca acagactgaa
    13201 tatagcgcgc attaaaaaat tcatattatt ctccatgctc atttttcttc agcttgcgca
    13261 tcagaagcca catacgtact gtcctgactt ctaaatcttt atcagagacg gattccttca
    13321 ccaggtcagc atgatgctga tagccattaa agaagacgtt cagcacaacg gcgctaaggg
    13381 ttgcgagcat aatgccgcta tgtagcagcg gttgcagtac ggccggtaac ttagaaaaga
    13441 aatcgtgaga gagcgtcgga gtcatgccaa cgccgagact gatcgccaca atatagaggt
    13501 tgtaacggtt ggtggtgtag ttacagcgcg acagaattcg aatccctgtc gccagtacca
    13561 tgccgaacat cactagacca gcgccgccca gcacaaattg cggaatggag gctaccagca
    13621 ccgccatttt tggcaccatg ccaaacagga ttaatataat tcccgaagaa atacacaccc
    13681 agcggctatg aacgcgcatc acgctaacca ggccaacgtt ttgagaaaat gacgtgtggg
    13741 ggaagctgtt aaacgtgccg cctatcattg tccctacgcc atcgacacgc agcccgcaaa
    13801 taatatcgtg tgaagagagt ttacgaccga ctatttcacc cagtgccagg aacatcccca
    13861 tcgactcgat aaacacgatg attaacacgg cagtcatggt cagaatggaa acgggatcga
    13921 aaatcggcat accaaacgac atcggggtga caatcgcaaa ccacgaggca tcatgtagcc
    13981 cggataaatt gacttcattc atcatccacg aaagtaaaaa gccaaataca atccccagta
    14041 atacggcgac gttggacata aatcctttcg catagcgagt aatgagcaag ataaaaatta
    14101 agacggcaaa ggagatacct aaataaacgg gattaccata ttgcggattc cctttacctc
    14161 cagcggccca gtcaataccc acctgaatga tgctaagccc aatagaagta ataaccacac
    14221 cggtaaccag tggcgggaat aaaggcatca agcgaccaat aagtggcgct aataatgtgg
    14281 tgataaaacc cgcggcgata gtggcaccaa atatccccag caggccgata tccgggttca
    14341 tcccaatggc tatcattggt gttacagcag caaaggtcac cgacataatc accggcaggc
    14401 ggatccccat aaagcggccg ataccgatac attgcaataa tgtgacgatc ccgcagcaaa
    14461 agagatccga gctaatgagc atcgcaatag catcttttga gaggcccagt cggtcaccaa
    14521 tcataagagg aacagcgact gcacctgcgt acatgacaag gacgtgttgc agaccgagta
    14581 tgatcagctt tcctggtgat aatatgcggt caacctcatc agttgggcgg tcttgcccag
    14641 aagatgaggg aagttgggaa tctatggcgc tcatcagtgt ttctcctttc gcccgccaga
    14701 ataaaaagca accgtttacg ttaatgaaac tgtctaaaca catgctcgtg gagtgccagt
    14761 ttttgcgagg aaggggagtt agagacagga attgcggagg caatcacata atcgtgctta
    14821 aaccacgaaa atgacaagag aaactgacaa tggaattatc tgtaattttc tcatattgat
    14881 atttcattaa tcattctggc atgtcgcact ctcgcattta atcgttttta tctggatagc
    14941 gctcttttga tcggcaatgt tgaaataatg aaagcggcat agattgaggg gcaataattt
    15001 gaactcagtc gcaaaattat aattaaaacg gtcatgctac ctaatatatg cccgtttatt
    15061 gcatcgcgaa atttgagaag acataactca ttaaatgaaa gggatgcaaa tgaatacgtt
    15121 tatcgctgct gaagctgcgg aatgtatagg ctgtca
//
LOCUS       DAZOYF010000094        15137 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZOYF010000094 DAZOYF010000000
VERSION     DAZOYF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.094"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..579
                     /locus_tag="ACHUOF_002696"
     CDS             <1..579
                     /locus_tag="ACHUOF_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0611818.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="ACHUOF_002697"
     CDS             597..1235
                     /locus_tag="ACHUOF_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0611819.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHUOF_002698"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHUOF_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR0611820.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHUOF_002699"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHUOF_002699"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR0611821.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="ACHUOF_002700"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="ACHUOF_002700"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR0611822.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHUOF_002701"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHUOF_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR0611823.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="ACHUOF_002702"
     CDS             complement(5552..6613)
                     /locus_tag="ACHUOF_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR0611824.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="ACHUOF_002703"
     CDS             complement(6610..8007)
                     /locus_tag="ACHUOF_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR0611825.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="ACHUOF_002704"
     CDS             8163..9161
                     /locus_tag="ACHUOF_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0611826.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHUOF_002705"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHUOF_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="ACHUOF_002706"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="ACHUOF_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHUOF_002707"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHUOF_002707"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR0611827.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHUOF_002708"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHUOF_002708"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR0611828.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..15137)
                     /locus_tag="ACHUOF_002709"
                     /pseudo
     CDS             complement(14232..15137)
                     /locus_tag="ACHUOF_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
BASE COUNT         3695 a         4144 c         3638 g         3660 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagc
//
LOCUS       DAZOYF010000095        15136 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZOYF010000095 DAZOYF010000000
VERSION     DAZOYF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.095"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="ACHUOF_002710"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="ACHUOF_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR0611829.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACHUOF_002711"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACHUOF_002711"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR0611830.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="ACHUOF_002712"
     CDS             1531..2478
                     /locus_tag="ACHUOF_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFR0611831.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="ACHUOF_002713"
     CDS             2548..3354
                     /locus_tag="ACHUOF_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0611832.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="ACHUOF_002714"
     CDS             3364..3771
                     /locus_tag="ACHUOF_002714"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR0611833.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3771..4265
                     /locus_tag="ACHUOF_002715"
     CDS             3771..4265
                     /locus_tag="ACHUOF_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFR0611834.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="ACHUOF_002716"
     CDS             4331..5128
                     /locus_tag="ACHUOF_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR0611835.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="ACHUOF_002717"
     CDS             5139..5927
                     /locus_tag="ACHUOF_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IID"
                     /protein_id="HFR0611836.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="ACHUOF_002718"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="ACHUOF_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFR0611837.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="ACHUOF_002719"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="ACHUOF_002719"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR0611838.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="ACHUOF_002720"
     CDS             complement(8370..10001)
                     /locus_tag="ACHUOF_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR0611839.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="ACHUOF_002721"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="ACHUOF_002721"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR0611840.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="ACHUOF_002722"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="ACHUOF_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR0611841.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3599 a         3993 c         3945 g         3599 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggtgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc acacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gtggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcaga ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatggt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gaggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 cttaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaatc gcaagatctc attccaccga
    15121 aacttttacg gctgca
//
LOCUS       DAZOYF010000096        14933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZOYF010000096 DAZOYF010000000
VERSION     DAZOYF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.096"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            123..365
                     /gene="yjbE"
                     /locus_tag="ACHUOF_002723"
     CDS             123..365
                     /gene="yjbE"
                     /locus_tag="ACHUOF_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR0611842.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            479..1117
                     /locus_tag="ACHUOF_002724"
     CDS             479..1117
                     /locus_tag="ACHUOF_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR0611843.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1114..1851
                     /locus_tag="ACHUOF_002725"
     CDS             1114..1851
                     /locus_tag="ACHUOF_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0611844.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1851..3947
                     /locus_tag="ACHUOF_002726"
     CDS             1851..3947
                     /locus_tag="ACHUOF_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0611845.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3994..4272)
                     /locus_tag="ACHUOF_002727"
     CDS             complement(3994..4272)
                     /locus_tag="ACHUOF_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611846.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4273..4416
                     /locus_tag="ACHUOF_002728"
     CDS             4273..4416
                     /locus_tag="ACHUOF_002728"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611847.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4542..4952
                     /gene="psiE"
                     /locus_tag="ACHUOF_002729"
     CDS             4542..4952
                     /gene="psiE"
                     /locus_tag="ACHUOF_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR0611848.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4996..6471)
                     /gene="xylE"
                     /locus_tag="ACHUOF_002730"
     CDS             complement(4996..6471)
                     /gene="xylE"
                     /locus_tag="ACHUOF_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR0611849.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6842..7732)
                     /gene="malG"
                     /locus_tag="ACHUOF_002731"
     CDS             complement(6842..7732)
                     /gene="malG"
                     /locus_tag="ACHUOF_002731"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR0611850.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7747..9291)
                     /gene="malF"
                     /locus_tag="ACHUOF_002732"
     CDS             complement(7747..9291)
                     /gene="malF"
                     /locus_tag="ACHUOF_002732"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR0611851.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9445..10635)
                     /gene="malE"
                     /locus_tag="ACHUOF_002733"
     CDS             complement(9445..10635)
                     /gene="malE"
                     /locus_tag="ACHUOF_002733"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR0611852.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11000..12115
                     /gene="malK"
                     /locus_tag="ACHUOF_002734"
     CDS             11000..12115
                     /gene="malK"
                     /locus_tag="ACHUOF_002734"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR0611853.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12187..13527
                     /gene="lamB"
                     /locus_tag="ACHUOF_002735"
     CDS             12187..13527
                     /gene="lamB"
                     /locus_tag="ACHUOF_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR0611854.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13679..14599
                     /gene="malM"
                     /locus_tag="ACHUOF_002736"
     CDS             13679..14599
                     /gene="malM"
                     /locus_tag="ACHUOF_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR0611855.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14828..>14933
                     /locus_tag="ACHUOF_002737"
     CDS             14828..>14933
                     /locus_tag="ACHUOF_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611856.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFN"
BASE COUNT         3704 a         3839 c         3912 g         3478 t
ORIGIN      
        1 ttatcctaaa actccatcgc taattccccg cgccgtaatt cgcatgcttt agttgtgtat
       61 actcgatccc gcccgaaatg tttttgggta aatctccatt cattcaatga agggaaattg
      121 ttatgaaaaa agttctgtat ggcatttttg ccatatctgc gcttgcggcg acttctgcgt
      181 gggctgcacc tgtacaggtg ggcgaagcgg cagagtcggc agcaacgtcg gtttcggcgg
      241 ggagttcctc cgcgaccagc gtcagcaccg taagctcggc ggtgggtgtc gcgctcgcgg
      301 caaccggtgg cggtgatggt tctaataccg ggaccaccac aaccacgacc accagtaccc
      361 agtaataaag tatgtatccc caaaataatt tgagtcattg catctgtggc ttgaagtatg
      421 aagggattta ccataaccac actccggtgt ggttattctg cccctctgga gaagagtcgt
      481 gaagcgacct gcactcattc ttatctgcct gctattacag gcctgttcag ccacgactaa
      541 agagctgggc aattcactgt gggacagtct gttcggcacg ccaggcgtac agctgacgga
      601 cgatgatatt caaaatatgc cctacgccag ccagtacatg cagcttaatg gcgggccgca
      661 attgtttgtg gtgctggcct tcgctgaaga cggacaacaa aaatgggtca ctcaggatca
      721 agccactctt gtcacacaac atggtcgtct ggtgaagact ttgcttggcg gcgacaacct
      781 gattgaagtg aataaccttg ccgccgaccc gctgattaaa cccgcacaaa ttgttgatgg
      841 tgcaacatgg acgcgcacga tgggctggac cgagtaccag caggtacgct acgccaccgc
      901 acgctcagtc ttcaaatggg atggcaccga taccgtcaag gtcggcagcg atgaaaccct
      961 ggttcgcgtg ctggacgaag aagtctccac cgaccaggcg cgctggcata accgctactg
     1021 gatcgacagc gaagggcaaa ttcgccagtc ggaacagtat ctcggcgcgg attatttccc
     1081 ggtgaaaacc actctcatca aggcggcaaa acaatgatta aacaaactat tgtcgcgttg
     1141 cttttgagtg tgggagcgtc atcggtcttc gcggcaggaa ccgtcaaggt gttcagcaat
     1201 ggcagcagtg aggccaaaac gctaacgggc gcagagcatt taatcgatct ggtagggcaa
     1261 cctcgactgg caaacagctg gtggcctggt gcggtgatta gcgaagagct ggcaacggcg
     1321 gcagcattgc gtcagcagca ggcgttgctg acacggctgg cagaacaggg cgcagattcc
     1381 agcaccgacg atgccgccgc cattaacgcc ttacgccagc aaatccaggc attgaaagtg
     1441 acaggcaggc aaaaaatcaa tcttgatccc gatatcgtcc gcgttgccga acgcggtaac
     1501 ccgccgttgc agggcaacta cacgctgtgg gtcggaccac cgccgtccac ggtcacgttg
     1561 ttcgggctta tcagccatcc tggcaatcag ccattcactc caggtcgcga cgtggcgagc
     1621 tatctctctg accagagcct gctcagcggt gcggatcgca gctacgcgtg ggtcgtttac
     1681 ccggacggac gcacgcaaaa agcgccggtg gcttactgga acaagcgtca cgtagagccg
     1741 atgcccggca gcattattta tgttggcctc gcagattccg tctggagtga gacgcctgat
     1801 gcccttaacg ccgacattct tcagactctg acgcagcgga taccccaata atgaaaaaaa
     1861 gacatctgct tagcttactg gcgctgggca ttagcacagc ttgctacggc gaaacatatc
     1921 ctgcgcccat tggtccgtcg cagtcggatt tcggtggcgt aggattatta caaactccca
     1981 ccgcgcgtat ggcacgggaa ggggagttga gtctgaacta tcgcgataac gatcagtacc
     2041 gttattactc agcttcagtg caactcttcc cgtggctgga aacaacgctg cgctacaccg
     2101 acgtgcgcac ccggcagtac agcagcgtcg aagcgttctc tggcgatcaa acgtataaag
     2161 ataaagcctt cgatctcaaa ctgcgtttgt gggaagagag ttactggctg ccgcaagtgg
     2221 cggttggtgc gcgggatatc ggcggtacgg ggctgtttga tgcggaatat cttgttgcca
     2281 gcaaagcctg ggggccgttc gattttacgc tcggcctggg ctggggatat ctgggcacca
     2341 gcggtaatgt aaaaaatccg ctctgttcag ccagtgataa atattgctat cgcgataaca
     2401 gctacaaaca ggcgggatct atcgacggta gccagatgtt ccacggtcct gcctcactgt
     2461 ttggcggcgt ggaataccag acgccctggc aaccgctgcg tctgaaactg gagtatgaag
     2521 gtaataatta tcagcaggat tttgccggga agctggagca aaaaagtaag tttaacgtcg
     2581 gggcgattta tcgcgttacc gattgggccg acgttaacct tagctatgaa cgtggcaaca
     2641 cctttatgtt tggcgtcacg ctgcgcacca actttaacga tctgcgcccg tcctacatcg
     2701 ataacgcccg cccgcaatat caaccgcagc cgcaggatgc cattttgcag cattcggtgg
     2761 tggcgaatca gttaacgttg ctgaaataca acgccggact tgccgatccg cagatccagg
     2821 cgaaaggcga tacgctgtat gttaccggcg agcaggtgaa atatcgtgat tcgcgcgaag
     2881 ggatcatccg tgctaatcgg atcgtgatga acgatctgcc ggatgggatc aaaacgatcc
     2941 gcattacgga aaatcgcctt aacatgccgc aagtgacgac ggaaaccgat gtcgccagcc
     3001 tgaaaaatca tctcgccgga gagccgttgg gccacgaaac gacgctggcg cacaaacgcg
     3061 tcgagccagt ggttccgaag tccaccgagc agggctggta tatcgacaaa tcacgctttg
     3121 atttccatat cgatccggtg ctgaaccagt cggtcggtgg cccggaaaac ttttacatgt
     3181 atcagctggg cgtgatggga acggcagatt tgtggctgac ggaccatcta ctgaccaccg
     3241 gcagcctgtt tgcaaatctt gccaacaact acgacaagtt taactacacc aatccaccgc
     3301 aggactcgca cttaccgcgc gttcgtaccc atgtgcgcga gtatgtgcag aacgatgtct
     3361 atgtgaataa cctgcaagcc aactacttcc agcatctggg caacggcttc tacggtcagg
     3421 tctatggagg ttatctcgaa accatgtttg gcggtgcggg ggccgaagtg ttgtatcgtc
     3481 cgctggacag taactgggcg tttggtctgg atgccaacta cgttaaacag cgcgactggc
     3541 gtagtgcaaa agatatgatg aaattcaccg actacagcgt gaaaaccgga catctgaccg
     3601 cctactggac gccatctttc gcccaggacg tgttggtgaa agccagcgtc gggcagtatc
     3661 tggcagggga taaaggcggc acgctggaga tcgctaaacg ctttgacagc ggcgtggtgg
     3721 tgggtggcta tgccacgatc accaacgttt cgaaagagga gtacggcgaa ggggacttca
     3781 ccaaaggcgt gtatgtctcg gtaccgttgg atctcttctc gtctggcccg acacgcagcc
     3841 gtgcggcgat tggctggacg ccgctgacgc gtgacggtgg tcagcaactt gggcgtaagt
     3901 tccagttgta tgacatgacc agcgaccgta gcgtaaattt ccgctaagtc atgggaaagg
     3961 tgccagtttt cgcactcact ggcaccctca ttcttaatga cctgttgagc catcaggaac
     4021 gcagttcacc cccggtttac gatctggtgt gtaaaggcga aaacattctg tcttcctgtc
     4081 cgtcgatatc ggttgcaggt ctggttgcct gagcgactgg gccatcattg ggctggagac
     4141 attgctttgc ggtaggtcgg ctttatcagc agtaccgagc ggaccagcat aagcaggaag
     4201 aacagagact gataacatca aagcagcaaa ataaggcttc atttttacca cctttatcag
     4261 gttacgtttc atttgttcca gaggaacatt gtcgattttt cgcgcattgc tggtggctgg
     4321 gaatcacctg aatgggtgat ttttgaatta ccggctttgg tgcggtttgt cttgccggat
     4381 gcgccgccag gcgcggctta tccggcctac gggtaggtat atccggcttt ggtggaggcg
     4441 cgctccaaat ccaggttgaa caaaacatac acaaaaaata tagatctccg tcacattttt
     4501 gcgttataca ggaagctcgc cactgtgaag gaggtactgc tatgacgtca ctctctcgtc
     4561 cgcgcgtgga gtttatctcc accattttgc agaccgtact caatcttggc ctgctgtgcc
     4621 tcggcctgat tttggttgtc ttcctcggca aagaaacggt gcatctggct gatgtgctgt
     4681 tcgcgccaga acaaaccagc aaatatgagc tggtagaagg actggtggtt tactttctct
     4741 atttcgaatt tatcgcgctg attgtgaagt actttcagtc cggttttcac ttcccgctac
     4801 gttactttgt ctatattggg atcaccgcca ttgtgcgctt gatcatcgtc gatcataaat
     4861 cgccactgga tgtgttgatc tactcggctg cgatcctgtt actggtgatc accctgtggc
     4921 tgtgtaattc gaaacggctg aagcgggagt aaaaagacag cacgccgaaa tggcgcggcg
     4981 tgctggacag gaagattaca gcgtagcagt ttgttgtgtt ttcttcgttt ccggttccca
     5041 gagcgcttcc agctcctcaa gggttttacc tttggtttcc gggacaaatt tccacataaa
     5101 cagtgctgcc agaacgccca tacaaccgta aatccagtag gagaaaccgt tgtggaaatg
     5161 ggccaccagc caggagtttt tgtccatcat cgggaaggtc caggagacga agtagttcgc
     5221 cagccactgg gccgccaccg cgattgccag cgctttacca cgaatagcat tcgggaagat
     5281 ttccgacagc agtacccagc ataccggacc ccaggacatg gcaaaggcgg caacatagaa
     5341 cagcatcgac agtagcgcca caatacccga tgcctgagtg taaaacgcgg taccgaggct
     5401 aaacataccg attgccattc cgagtgcgcc gataatttgc agtggcttac gaccaaattt
     5461 atccacagtc ataattgcca gtacggtaaa ggtgaggttg ataactccga caataatggt
     5521 ctgcaacagc gcgatatccg tgctggcccc cagcgttttg aacacttccg gcgcgtagta
     5581 cagcaccaca ttgatgccga caaattgctg gaagatggag agcataacgc cgattacaat
     5641 cacgcccacg ccaaacatca gcagacgacc accggttttg cggccatgat ccagggagtg
     5701 tttaatttcc tgtactgcct gagttgcaag cgtgttgccc ataattttgc gcaggatact
     5761 ttccgtctgt tcttgcttgc cgcgtgacat cagccagcga ggactttctg gcacggtata
     5821 cagcagcatt aagaacagca gtgcagggat acattccgag gcaaacatat aacgcctgcc
     5881 gtcagtattc agccagctgg catcaccgga acgggcaata aaatagttta cgcagtaaac
     5941 taaaagttgc ccgaaaataa tcgcaaactg gttaaatgaa accagttttc cgcgaatatg
     6001 agctggagcc agttccgcaa tatacattgg tgaaagcatt gaggctaaac caacgccaat
     6061 accgccaata atacgataaa taacaaattc cgggacataa cctgccagat aaataggtac
     6121 tgtgttgtcc gggtttatag aggtaaaacc aagttctggc caggcagaac ctacaccaga
     6181 aataaaaaac aggacagcag aaatcttaag tgaatcacga cggccgaagc ggttactgca
     6241 ataaccaccg agggcaccgc cgatgatgca accaatcaga gcgctggcta cgcaaaatcc
     6301 taacagggag ttggcagcgg attcacttaa gttttgtgga gcaacaaaga cggtattgag
     6361 tgactcaaca gtaccggaaa taacggcggt gtcgtagcca aataataaac cacctaatgt
     6421 agcgactaag gtaatcgaaa atatataact ggaattatac tgggtattca ttcagacctg
     6481 ccttagacca ttctgatctt ttatcagtgc attgatgttg gaattgtcag gcagttaagg
     6541 caattatcat tttttacaat gcgtttacgt aatgtctttt ctgtgatctt aattgtgata
     6601 attatcaaaa attgatgaaa caatactatt gccgtgactc agagcacgaa agagaattat
     6661 cgtaagtggg aaaacaaata acgtaaaaat aataagttct atgatgggaa tattaaccgg
     6721 cgaacgattc agattgcaga cgaaagaaaa aaaggcgctc cgtggagcgc cgaataatag
     6781 tcacaagttg ggataacgta agttgagggt gcagcggcat aacattggca gaacaacatc
     6841 tttaaccttt cacaccacct gccgtcaggc cgttgaccag ccagcgttga gccagcaaga
     6901 agacgatggt gatcggtaat gcagacatca cggcagcggc ggcaaagtca ccccacaggt
     6961 agttttgcgg gttgaggtat tgctgcatcc ccacggccag ggtgtagctg tttacgtcac
     7021 gcagtaacag cgacgcgacc ggaacttcag taatggcagc gataaacgac aggatgaata
     7081 ccaccgccag aatcggtact gacagcggca acaggacaag gcggaaggcc tgccacggtg
     7141 tcgcaccatc cagcgcagcc gcttcttcca gcgaactgtc gatggtttcg aaatagcctt
     7201 tgatggtcca gacatgcagc gcaatcccac ccagatacgc gaaaattacg ccgccgtgag
     7261 tattcaggcc aatgaatgga atgtactcac ccagacgatc aaacaacgca tacaacgcga
     7321 ccagtgaaag tactgccggg aacatctgga aaatcagcat tcctttcagc agcgtcgctt
     7381 tgcctggaaa gcgcatacgg gcgaaagcgt aggcgcaggt ggtggagagc gccacaatgc
     7441 caatcgcgga aatcccagcg acctttaccg agttccacag ccacagcagt accgggaatg
     7501 gcggtggcgt aatgcgacca tcagcctgtt caacgctaaa acctaacgcc agtttccagt
     7561 gatcccagga gatttgctcc gggatcaggc taccggtcgc gaagtttccc tgacgcagcg
     7621 agatagcgac gaccatcagc agcgggaaca taatcgccgc gataaaaagt agcagtagca
     7681 ggtgagtaat aaacaaacgt gctttttgcg atttcggttg gaccattgcc atttttgtta
     7741 tctcccttaa tcaaacttca ttcgcgtggc tttcaggttc actatcgcca gcgcacccac
     7801 cagcaggaag atcagcgtgg caattgctgc cgccagaccg aagtcctgac ccccgccgcc
     7861 ttcaaaagcg atacggtagg tgtagttaac aagcaggtcg gtataaccgg ctggcgtggt
     7921 cgtgccaaga cgatccgggc cgccgttggt taacagctga atcagcacga agttgttaaa
     7981 gttaaaggcg aagctggcga tcatcagcgg cgtcagcggt ttaatcagca gcggcagcgt
     8041 aatcttaaag aagttctgga acggacctgc gccatccatt gctgaggctt catacaaatc
     8101 gtccggaatc gctttcagca agcccatgca gaggatcatc atgtacggat aacccagcca
     8161 ggtattgacg ataatcagca tcgtgcgagc ggtggtcgga tcgctgaacc aggcgggctt
     8221 cacgccaaac agcgcgctca acatcatgtt gatttcaccg aagctctggt taaacaatcc
     8281 tttgaaaatc aagattgaaa tgaacgatgg caccgcgtag ggcagaatca gcaggacgcg
     8341 atagaccgct ttgccgcgca acgcttccca ctgcaccaga catgccagaa ccatgccgac
     8401 cgccaccgtt aaaaagacag tgatcagcga gaacaccacg gtccagacga aaatggcgag
     8461 gaacggtttc tgaatgcctt cgtcggtaaa gacgcgggta aagtttttcc agccggtggt
     8521 cacggtgtaa ccggggctta gcttttcatc cccccagttg ccgtcggcgg taatggactg
     8581 gtaaaagcca atttggttat tcggacgata tttcacgcca ctctgattat tcgtcaacgt
     8641 gccgtcaccg tcgagtgtgt agagcggctg cgtgccagaa aactggcgca gggagctcat
     8701 catcactttg ttgccatccg gcagaatggc ggtaatgtca ctcagcgcct gacgattctg
     8761 ggtaatcacg cgcagattcg cgcgttcgcc ttcgggctgg gcggtcgttt ctttcagttg
     8821 cagtttttgc tcgccgccaa atttaaacgc gtcggagagg taatttttgc cggtttcgcc
     8881 gtcgctgagc gccagttgcc actcatcgcc cgccgggtaa agaccaaagt tataggtttt
     8941 gcctgcttgc caggagcgat ctaacaacac ttcctgcgca cgttcaaaag tcagctggtt
     9001 agtgctgctg tagttggtga aggcaatggc gatggtgcag accagaggga agaggacgaa
     9061 taatcccatt ccagccattc ccgggtaaac atagcgccag gcgtaggctt tacgattggc
     9121 gaaaatatac agccccgctg aactcaatat cagcgtggta atggcgaaca ggtattcccc
     9181 ttgtgcgtac attaaaacaa caaggtaacc caccagcagg ccgagcagac ctagcactga
     9241 ccatttcagc gcgtcgcttt gccaccaatg tttcttttta atgacatcca tggggttctt
     9301 cctcattcca ggacggataa ggctttcacg ccttatccga caacaactgc ctgatgcgac
     9361 actgatgcgt cttatcaggc ctacatgcgt ttcagttttg taggccggac aaggcgttca
     9421 cgccgcatcc ggcatttcac agcattactt ggtgatacga gtctgcgcgt ctttcagggc
     9481 ttcatcgaca gtctgacgac cgctggcggc gttgatcacc gcagtacgta cggcatacca
     9541 gaaagcggac atctgcggga tgttcggcat gatttcacct ttctgggcgt tttccatggt
     9601 ggcggcaata cgtggatctt tcgccaactc ttcctcgtaa gacttcagcg ctacggcacc
     9661 cagcggtttg tctttattaa ccgcttccag accttcatca gtcagcagat agttttcgag
     9721 gaactctttc gccagctctt tgttcggact ggcggcgtta ataccggtgc tcagcacgcc
     9781 aacgaacggt ttagatggtt gacccttgaa ggtcggcagt accgttacac cataattcac
     9841 tttgctggtg tcgatgttgg accatgccca cgggccgttg atggtcatcg ctgtttcgcc
     9901 tttattaaag gcagcttctg cgatggagta atcggtgtct gcattcatgt gtttgttttt
     9961 aatcaggtca accaggaagg tcagacccgc tttcgcgcca gcgttatcca cgcccacgtc
    10021 tttaatgtcg tacttgccgt tttcatactt gaacgcataa cccccgtcag cagcaatcag
    10081 cggccaggtg aagtacggtt cttgcaggtt gaacatcagc gcgctcttac ctttcgcttt
    10141 cagttcttta tccagcgccg ggatctcttc ccaggttttt ggcgggttcg gcagcaggtc
    10201 tttgttataa atcagcgata acgcttcaac agcgattggg taagcaatca gcttgccgtt
    10261 gtaacgtacg gcatcccatg taaacggata cagcttgtcc tggaacgctt tgtccggggt
    10321 gatttcagcc aacaggccag attgagcgta gccaccaaag cggtcgtgcg cccagaagat
    10381 aatgtcaggg ccatcgccag ttgccgcaac ctgagggaat ttctcttcca gtttatccgg
    10441 atgctcaacg gtgactttaa ttccggtatc tttctcgaat ttcttaccga cttcagcgag
    10501 accgttatag cctttatcgc cgttaatcca gattaccagt ttaccttctt cgattttggc
    10561 gagagccgag gcggaaaaca tcatcgtcgt taatgcggat aatgcgagga tgcgtgcacc
    10621 tgtttttatt ttcataatct atggtccttg ttggtgaagt gctcgtgaaa acacctaaac
    10681 ggactctagt ttctttatac ggcaacctct ttccatcctc cttgccccta cgccccaccg
    10741 ttgctttgtg tgatctctgt tacagaattg gcggtaatgt ggggatgcgc acataaaatc
    10801 gccacgattt ttgcaagcaa catcacgaaa ttccttacat gacctcggtt tagttcacag
    10861 aagccgcgtt ctcatcctcc cgcctcctcc cccataaaaa agccaggggg tggaggattt
    10921 aagccatctc ctgatgacgc atagtcagcc catcatgaat gttgctgtcg atgacaggtt
    10981 gttacaaagg gagaagggca tggcgagcgt acagctgcaa aatgtaacga aagcctgggg
    11041 cgaggtcgtg gtatcgaaag atatcaatct cgatatccat gaaggtgaat tcgtggtgtt
    11101 tgtcggaccg tctggctgcg gtaaatcgac tttactgcgc atgattgccg ggcttgagac
    11161 gatcaccagc ggcgacctgt tcatcggtga gaaacggatg aatgacactc cgccagcaga
    11221 acgcggcgtt ggtatggtgt ttcagtctta cgcgctctat ccccacctgt cagtagcaga
    11281 aaacatgtca tttggcctga aactggctgg cgcaaaaaaa gaggtgatta accaacgcgt
    11341 caaccaggtg gcggaagtgc tgcaactggc gcatttgctg gatcgcaaac cgaaagcgct
    11401 ctccggtggt cagcgtcagc gcgtggcgat tggccgtacg ctggtggccg agccaagcgt
    11461 atttttgctc gatgaaccgc tctccaacct cgatgctgca ctgcgtgtgc aaatgcgtat
    11521 cgaaatctcc cgtctgcata aacgcctggg ccgcacaatg atttacgtca cccacgatca
    11581 ggtcgaagcg atgacgctgg ccgacaaaat cgtggtgctg gacgccggtc gcgtggcaca
    11641 ggttgggaaa ccgctggaac tgtaccacta tccggcagac cgttttgtcg ccggatttat
    11701 cggttcgcca aagatgaact tcctgccggt aaaagtgacc gccaccgcaa tcgatcaagt
    11761 gcaggtggag ctgccgatgc caaatcgtca acaagtctgg ctgcctgttg aaagccgtga
    11821 tgtccaggtt ggagccaata tgtcgctggg tattcgcccg gaacatctac tgccgagtga
    11881 tatcgctgac gtcatccttg agggtgaagt tcaggtcgtc gagcaactcg gcaacgaaac
    11941 ccaaatctat atccagatcc cttccattcg tcaaaacctg gtgtaccgcc agaacgacgt
    12001 ggtgttggta gaagaaggtg ccacattcgc tatcggcctg ccgccagagc gttgccatct
    12061 gttccgtgag gatggcactg catgtcgtcg actgcataag gagccgggcg tttaagcacc
    12121 ccacaaaaca cacaaagcct gtcacaggtg atgtgaaaaa agaaaagcaa tgactcagga
    12181 gatagaatga tgattactct gcgcaaactt cctctggcgg ttgccgtcgc agcgggcgta
    12241 atgtctgctc aggcaatggc tgttgatttc cacggctatg cacgttccgg tattggctgg
    12301 acaggtagcg gcggtgaaca acagtgtttc cagactaccg gtgctcaaag taaataccgt
    12361 cttggcaacg aatgtgaaac ttatgctgaa ttaaaattgg gtcaggaagt gtggaaagag
    12421 ggcgataaga gcttctattt cgacactaac gtggcctatt ccgtcgcgca acagaatgac
    12481 tgggaagcta ccgatccggc cttccgtgaa gcaaacgtgc agggaaaaaa cctgatcgaa
    12541 tggctgccag gttccaccat ctgggcaggt aagcgcttct accaacgtca tgacgttcat
    12601 atgatcgact tctactactg ggatatttct ggtcctggtg ccggtctgga aaacatcgat
    12661 gttggcttcg gtaaactctc tctggcagca acccgctcct ctgaagctgg tggttcttcc
    12721 tcttttgcca gcaacaatat ttatgactat accaacgaaa ccgcgaacga cgttttcgat
    12781 gtgcgtttag cgcagatgga aatcaacccg ggcggcacat tagaactggg tgtcgactac
    12841 ggtcgtgcca acctgcgtga taactatcgt ctggttgatg gcgcatcgaa agacggctgg
    12901 ttattcactg ctgaacatac tcagagtgtc ctgaagggct ttaacaagtt tgttgttcag
    12961 tacgctactg actcgatgac ctcgcagggt aaaggtctgt cgcagggttc tggcgtcgcg
    13021 tttgataacg aaaaatttgc ctacaatatc aacaacaacg gtcacatgct gcgtatcctc
    13081 gaccacggtg cgatctccat gggcgacaac tgggacatga tgtacgtggg tatgtaccag
    13141 gatatcaact gggataacga caacggcacc aagtggtgga ccgttggtat tcgcccgatg
    13201 tacaagtgga cgccaatcat gagcaccgtg atggaaatcg gctacgacaa cgtcgaatcc
    13261 cagcgcaccg gcgacaagaa caatcagtac aaaattaccc tcgcacaaca atggcaggct
    13321 ggcgacagca tctggtcacg cccggctatt cgtgtcttcg caacctacgc caagtgggat
    13381 gagaaatggg gttacgacta caacggcgat agcaaggtta acccgaacta cggcaaagcc
    13441 gttcctgctg atttcaacgg cggcagcttc ggtcgtggcg acagcgacga gtggaccttc
    13501 ggtgcccaga tggaaatctg gtggtaatag caaaacctgg gccggataag gcgtttacgc
    13561 cgcatccggc ataaaaacag gttgtcatta tctgaaaggg gcgaaagccc ctctgattat
    13621 cgggtttagc gcgctattgc ctggctaccg ctgagctcca gattttgagg tgaaaacaat
    13681 gaaaatgaat aaaagtctca tcgccctctg tttatcagca gggttactgg caagcgcgcc
    13741 tggaattagc cttgccgatg ttaactacgt accgcaaaac accagcgacg cgccagccat
    13801 tccatctgcc gcgctgcaac aactcacctg gacaccggtc gatcaatcta aaacccagac
    13861 cacccaactg gcgaccggcg gccaacaact gaacgttccc ggcatcagtg gtccggttgc
    13921 tgcgtacagc gtcccggcaa acattggcga actgaccctg acgctgacca gcgaagtgaa
    13981 caaacaaacc agcgtttttg cgccgaacgt gctgattctt gatcagaaca tgaccccatc
    14041 agccttcttc cccagcagtt atttcaccta ccaggaacta ggcgtgatga gtgcagatcg
    14101 gctggaaggc gttatgcgcc tgacaccggc gttggggcag caaaaacttt atgttctggt
    14161 ctttaccacg gaaaaagatc tccagcagac gacccaactg ctcgacccgg ctaaagccta
    14221 tgccaaaggc gtcggtaact cgatcccgga tattcccgat ccggttgctc gtcataccag
    14281 cgatggctta ctgaaactga aagtcaaaac gaactccagc tccagcgtgt tggtaggacc
    14341 gctatttggt tcttccgctc cagctccggt tacggtaggc aacacggcgg caccagctgt
    14401 ggctgcacct gctccggtgc cggtgaagaa aagcgagccg atgctcaacg acacggaaag
    14461 ttattttaat accgcgatca aaaacgctgt cgcgaaaggt gatgttgata aggcgttaaa
    14521 actgcttgat gaagctgaac gcctgggatc gacatctgcc cgttccacct ttatcagcag
    14581 tgtaaaaggc aaggggtaat tacgccccac agtgctgatt ttgcaacaac tggtgcgtct
    14641 cctggcgcac ctttttttat gcttccttcc tgggatatga gcgatttttt atagtacttc
    14701 acttcttctt cactaagaat gtccattatt tcaatgcatt atcagagatt cttttccttt
    14761 cgccggtagt gtctggacgc tcaggctact tttccaggtt attttatttc tgtattacag
    14821 aggttttatg ataggtcata tcacaaattc tggcggcaat aactctttga tgaaacatga
    14881 tgtggtgcaa ggaaataata tagtagatct tgatttacta cgtaatttca atg
//
LOCUS       DAZOYF010000097        14700 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZOYF010000097 DAZOYF010000000
VERSION     DAZOYF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.097"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(4..1266)
                     /locus_tag="ACHUOF_002738"
     CDS             complement(4..1266)
                     /locus_tag="ACHUOF_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0611857.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(1464..1539)
                     /locus_tag="ACHUOF_002739"
     tRNA            complement(1464..1539)
                     /locus_tag="ACHUOF_002739"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1504..1506),aa:Phe,seq:gaa)
     gene            complement(1645..2352)
                     /locus_tag="ACHUOF_002740"
     CDS             complement(1645..2352)
                     /locus_tag="ACHUOF_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR0611858.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2505..2588
                     /gene="yqgH"
                     /locus_tag="ACHUOF_002741"
     CDS             2505..2588
                     /gene="yqgH"
                     /locus_tag="ACHUOF_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR0611859.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2750..4885
                     /gene="speC"
                     /locus_tag="ACHUOF_002742"
     CDS             2750..4885
                     /gene="speC"
                     /locus_tag="ACHUOF_002742"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR0611860.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4934..6190)
                     /gene="nupG"
                     /locus_tag="ACHUOF_002743"
     CDS             complement(4934..6190)
                     /gene="nupG"
                     /locus_tag="ACHUOF_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR0611861.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6392..7471)
                     /gene="mltC"
                     /locus_tag="ACHUOF_002744"
     CDS             complement(6392..7471)
                     /gene="mltC"
                     /locus_tag="ACHUOF_002744"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR0611862.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7536..7811)
                     /locus_tag="ACHUOF_002745"
     CDS             complement(7536..7811)
                     /locus_tag="ACHUOF_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR0611863.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7839..8891)
                     /gene="mutY"
                     /locus_tag="ACHUOF_002746"
     CDS             complement(7839..8891)
                     /gene="mutY"
                     /locus_tag="ACHUOF_002746"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR0611864.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9052..9771
                     /gene="trmB"
                     /locus_tag="ACHUOF_002747"
     CDS             9052..9771
                     /gene="trmB"
                     /locus_tag="ACHUOF_002747"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR0611865.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9771..10097
                     /locus_tag="ACHUOF_002748"
     CDS             9771..10097
                     /locus_tag="ACHUOF_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR0611866.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10281..11000
                     /locus_tag="ACHUOF_002749"
     CDS             10281..11000
                     /locus_tag="ACHUOF_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR0611867.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11176..12222
                     /gene="ansB"
                     /locus_tag="ACHUOF_002750"
     CDS             11176..12222
                     /gene="ansB"
                     /locus_tag="ACHUOF_002750"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR0611868.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12339..13301
                     /locus_tag="ACHUOF_002751"
     CDS             12339..13301
                     /locus_tag="ACHUOF_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR0611869.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(13402..>14700)
                     /locus_tag="ACHUOF_002752"
     CDS             complement(13402..>14700)
                     /locus_tag="ACHUOF_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFR0611870.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3835 a         3548 c         3789 g         3528 t
ORIGIN      
        1 ttactatttt aatggattgt taatctttgc atattcaaac gggctgatac ccttctcccg
       61 gttcgcatct agaaaatcgg cccaccattg caacatcagt cgccgttctt ccagatgttc
      121 tgctttatgg atatacgcag cacgtacacc attacgctcc tgatggctca tctgacgttc
      181 cacagcatcg cgggaccaca aacctgattc aatcaatgca ctgcacgcca tcgcccggaa
      241 tccatggcca cagacttcct gggttgtgtc ataccccatc accctcaggg ctttatttac
      301 agtattttca ctgattggtt tacgcgggtc atgtgcaccc gtaaagatca gaccattttc
      361 accagcccat gtctgaagtt ctgccagtat ggctacaacc tgtttgctga gtggcacaag
      421 atgtggtgtt cgcatcttgg atccccggtg tgagaatttt acgcctggca gaggttcgcg
      481 ttcggctgga atagtccaca tggaattgtc aatatcaatt tccgaccagc gggcatatct
      541 gagttcactg gaacgaataa aaaccagtaa attcagcata actgccagtc gggtgacagg
      601 ctggccttta taaccgtcaa tacgcgacag aagttcaggc aggcgtgaaa gctccagagc
      661 agggcgatgc tggcgtttta ccgtggtcaa tgcaccagcc atatccagag ccgggttata
      721 tctgatgatg ccagactgta cggcgtaacg cattacagca gatattcttt gctgcagacg
      781 actggcgatt tcataaattt gttttgcttc tgcagcacga acagggataa gcaggtccgg
      841 ggtattgagt gttgtgatat cccggttgcc gataaagggg aaaacgtgtg tctcgaggct
      901 tttgagtact ttatcgctgt gtgattgtgc ccattgttta ttactggcat gccagcgtcg
      961 tgcaatatgt tcaaaagaga gctgtaaatc agggacaact tttttaactc ttttctgttc
     1021 gcaagggtca atcccctctg aaatcagttt tcgggcctca tcccgttttg tacgagcttc
     1081 ggagagagaa acttctggat acacaccaag tgccagagtc ttctccttac ccagaatacg
     1141 ataacggaga cgccagtaac gggaaccatt aggatgaacc agcaggaaca tgccggcccc
     1201 gtcagtgagt ttataagcct tgtcagtggg ctttgcagcc cggatttttg cgtcagtcag
     1261 tgccatagtt cagccttctg aatgcgttaa agagggtaca aattctgtcg aactggggta
     1321 tacccgcaat tatacccaca caatggggtt gatgtggggg gaatctggtt gagttcggta
     1381 gaattgattt ggaggaagaa cacttaaatc atggtgttct gaaacgaaaa acggacctcc
     1441 gtggaggtcc gttgatatga atttggtgcc cggactcgga atcgaaccaa ggacacgggg
     1501 attttcaatc ccctgctcta ccgactgagc tatccgggca acggggcgca ttaaacctga
     1561 ttcgcctcgt ctcgtcaatc aaatttcttc aatttactgc agactgcaca atctatcatc
     1621 actttgccgc tattgcacgc attgtcaggc aaaccaggcg gtccaggcgg cggaaagcgg
     1681 cattgccaga aggagtgctg gaagcatgtt gaccaccggg aacattttaa tgccacagat
     1741 gcgtaatccg gttgccagca gcaataaacc gcctacggcg ctgaagtctg ccatcatcga
     1801 cggtgtgatc agcggtaata tcagcgcggc agcccacgcc agcgttaact ggatgatcag
     1861 taatgggata ctaattaccg acaccgcaat acccagcgag caggcgaaga tcatcgccgt
     1921 aaagaaatca agaaatgact tggcgattaa aatactcgga tcgccggtca tcccttcgtt
     1981 catcgccccg aagatcccgg tgccgctggc gcaaaacagg acaataatcg cgacataatt
     2041 ctgaataaaa gattcatgcg ctggcttctt acgtgagtga cgaaacagat tttgtgcttt
     2101 ggcgactgct gtattgacac ctttctccag caaacaaatt tcgccaatta gagcgccaag
     2161 tagcgtagct aaaaccatcg ctggaaggtt ggcgcatttc acaaccagta aaataccaat
     2221 ccccagcgat gccagaccaa aaatagatgt catggagacg cggatacgtt ccggtaagcg
     2281 ttggctgagc agtgcgccga gaacgccacc cagtaagact gcactagcgt tgataaaagg
     2341 gccgatgacc acaagagttc ctgttagctg tattgtttga ttttcttatt atgaactttt
     2401 tgccttgttg atggcttgtc tggtcgcaaa gtcctggctt gcacgcctta gcgaaaggtg
     2461 ccatgattgc gcgaattttc tcctctctgt acggagtttg cccgatgcac gccatctcct
     2521 tacattctct cgcttatcgc cgtttcgcgc gaaacgtttc cctttctatg ttactgctca
     2581 tgcggtgaag ttaacgcacg ctcactgcag gacaacagta aaatcagagc gtttctgctt
     2641 ttactgatgt ctggcggtcg gagctggtga ccagtttgac ccatatctca tggggcaggg
     2701 ttttccacct tgtccggtat tcttacttcc ccgaaacggg tttgcgctta tgaaatcaat
     2761 gaatattgcc gccagtagtg aactggtatc ccgactttct tctcatcgcc gcgtggtggc
     2821 gttgggagat actgatttta cggacgtcgc ggcagtcgtc attaccgctg cggatagtcg
     2881 cagtggcatt cttgcgttgc ttaagcgcac cggttttcat ctaccggtgt ttttgtattc
     2941 cgaacatgct gttgaattac ctgcgggcgt tacggcggta atcaacggca acgagcagca
     3001 gtggctggag ctggaatccg cagcctgtca gtatgaagag aatttgctgc caccgtttta
     3061 tgacacgctg acgcagtacg ttgagatggg caacagcacc tttgcttgcc ctggacatca
     3121 acatggtgcg ttctttaaaa agcatcctgc cggacgccat ttttacgatt tctttggtga
     3181 gaacgtcttt cgcgccgata tgtgtaacgc tgacgtaaaa ttgggcgatc tgcttattca
     3241 tgaaggatcg gcgaaagatg cgcagaaatt cgcagccaaa gtctttcatg ccgataaaac
     3301 ctattttgtg ctgaacggca catcggcagc gaataaagtg gtgacgaatg cgctgttaac
     3361 gcgtggcgat ctggtgctct tcgaccgtaa caaccataag tcgaatcatc acggcgcgct
     3421 gattcaggcg gggacgacgc cggtctatct ggaagcttca cgtaacccgt ttggtttcat
     3481 tggcggtatt gatgcgcact gttttaatga agagtatctg cgccagcaaa ttcgcgacgt
     3541 tgcgccagaa aaagccgacc tgccgcgccc gtttcgcctg acgattattc agctgggaac
     3601 ctatgacggc actgtctata acgcccgtca ggtgatcgat accattgggc atctgtgtga
     3661 ttacattctg tttgattccg cgtgggtcgg ttacgaacag tttatctcga tgatggcgga
     3721 tagctcgccg ctgctgttag aacttaacga aaacgatccg gggatttttg tgacccagtt
     3781 ggtgcacaaa cagcaggctg gattctcaca gacgtcgcag atccataaaa aagataacca
     3841 tatccgcgga caggcgcgtt tttgcccgca taagcggttg aataacgcct ttatgctcca
     3901 tgcttctacc agccctttct atccgctgtt tgctgcactg gatgttaacg ccaaaattca
     3961 tgaaggggag agtgggcgtc ggctgtgggc tgagtgcgtt gagttgggta ttgagtcgcg
     4021 caaggctatt cttgcgcgct gtaagctgtt ccgcccgttt atcccgcccg ttgttgatgg
     4081 caaattgtgg caggattatc cgacatcagt gttagccagc gaccgccgtt ttttcagttt
     4141 tgagccgggg gcgaagtggc acggctttga aggatatgcc gcggatcagt attttgttga
     4201 tccgtgcaag ctgttactca ctacaccagg tatcaatgcc gaaaccggcg aatatagcga
     4261 ctttggcgtt ccggcgacga ttctggcgca ctatctgcgt gagaacggca ttgtgccgga
     4321 gaagtgcgat ctcaactcca ttctgttctt attaactccg gcggaaagcc acgagaagct
     4381 ggcacaactg gtggcgatgc tggcgcaatt tgaacagcat attgaggatg actcgtcgct
     4441 ggctgaggtg ttgccgagcg tttataacaa atatccggtg cgctatcgcg actacaccct
     4501 gcgccagttg tgtcaggaga tgcacgatct gtatgtcagt ttcgacgtca aagacctaca
     4561 aaaagcgatg ttccgccagc agagtttccc gtcagtggtg atgaaccccc aggatgcgca
     4621 tagcgcttat attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat
     4681 tgcggcagaa ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt
     4741 ctggggtggg gcggttcaac gttatttcct tgcgctggaa gaaggcgtga atttgttgcc
     4801 gggattttcg ccggagttgc aaggtgtcta tagcgaaacc gatgcggatg gcatgaaacg
     4861 attgtacggt tatgtgttga agtaagaata aaaaaacggg tcaccttctg gcgacccgtt
     4921 tttctttgcg taattagtgg ctaaccgtct gtgtgcctgt cgggacacga acgtgtttat
     4981 atttgaacat cgccatgaac gcgaaggcca gaaccacgga ataaccagcg aaaatcaacc
     5041 atacggtctg ccagtcggta atgccgtttt gggtgtacat ctcaacaact ttaccgctca
     5101 cgatgccgcc gaggatacag ccgaagccgt tagtcatcat caggaacatc ccttgtgcac
     5161 tggcgcgaat tgccgggcta acttcttttt cgacaaacac cgaaccagag atgttgaaga
     5221 agtcgaatgc gcaaccgtaa acgatcatcg acagtaccag cagtacagta ccgaacggag
     5281 tcgggtcgcc gtaagcaaac agcgcaaaac gcaggatcca cgccacaata ctgatcatca
     5341 ttacgttctt aataccgtag cggcttaaga agaacgggat ggtcagaatg aacagggttt
     5401 cagagatctg cgaaatcgac atgatgattg acgcatgctg cacgatgaag ctgctggcaa
     5461 acatcggatc tttgtcgaag ctgtgcagga aggtattacc gaacatgttg gtaatctgca
     5521 gttccgcgcc cagcagcatt gagaagatga agaagattgc catacgcttg tttttaaaca
     5581 gcgcgaatgc atcgaggccc agcagggttg tccagctctg attcgcttgc tgtttagcaa
     5641 ccggaatatg cggcagagtc agggtaaaca gaaccagaat ggcggaaagt gctgcgccaa
     5701 tatacagctg catgtggctt aattcgaagc cagacaggct caccacccac attgccatga
     5761 taaagccgat ggtgccccag atacggattg gcgggaagtc agtaacgata tccatcccgg
     5821 cattttgcag gcgatagtaa gagatggtgt tgattaaccc aagcgttggc atataagcaa
     5881 acgagttaat caatatcaca aggaacatcg cttccggcgt cgtgacctgt gccgccatga
     5941 acagcgtgat agcgccaatg gtgtggcaaa tggcatatac ccatttcgca cttaaccatt
     6001 tgtcggccac aatccccagc agcgcaggca taaataccgc ggcgataccc agtgagctat
     6061 aaactgcgcc aatagaagca ccgtcaaact tcagggtaac aaacatatag gagccgaggg
     6121 tcgtcagcca acttccccac agacagaact gcagaaaaga gaggattttc agctgcagct
     6181 taagattcat gttaatttcc tcacatcgtg atgcggatga gtgtttgaaa aagcacattt
     6241 gaccgtgcga gatgatggga ttctatcaac ttcgcggact ggttttttgt tacctgtggc
     6301 aaataattgc aaacattccc aatttgcaaa acgaaattgt gatgtggata acatttttgc
     6361 ccctgagcat cgtcaggggc ggttaatgga attatcggcg gcggtaggat ttttgcgcgg
     6421 tattcacttt ataaagataa cggcgagatt ccgcagaggg atggcgggtc gtcagcgtct
     6481 gataaacatc gcccggcgtc atggtgttaa taatattggc agcctgaatc ttatcattcg
     6541 aaaagactcg cagcacgctg cctgcgccgc cgttataggc ggtgatgacg gcataacgcc
     6601 gcgatgttgg gttatcaatt ccgccgagat aaacattgtt cagcatcgcc agatacgcgg
     6661 tgccggtatc aatattgctg gcaggatcaa acaagaaact gcggctcggc gtgccggatt
     6721 tcccctgcga gcggaacaca tctttcccgg cagtattttg taccacctgc attaatccca
     6781 gcgcatcgga acggctgacc gcatacgggt taaaggaaga ttcagtctgc ataattgcca
     6841 gaatcagcga ctcatcaacg ccatatttcc gtgacgcctg gcggaccatg ccgagatatt
     6901 tgtgcgcacg tttatcaagg tggttcggca ccatgttaat ggtgacgctg tagatgatac
     6961 gtagcccgtt gctgcggctt ttcagacggt ttttcagcag ataatccgcg aagttgcttg
     7021 cgcgaccttc ccagcgaatc ggctgcccgg tgttgtccac cacctgaccg taaaggaaag
     7081 gttctttcga aattgtaata tcatcaacgt cggaatagag atcgaccgaa ctcggatcgt
     7141 cacccatcag caacgttttg ataattgccc ggcgcaaatg cgcggcaggt tctgtcccgg
     7201 cgatggtttc gatagtaatc gtaccgtcat cgaagttgat gtggctgcgg gtctgatatt
     7261 gatcggtgta tttcacgtag tccttaggac cggcgatcac cacctctttg aagccccaga
     7321 tgttctcaat attgtgggca aattgcccca tcagaatatc aaaaccgttg gtatctttga
     7381 cccaggcttc gttataggta tcgccttttt tggtcgtcga acaggagatg agcaacggcg
     7441 caatcaaagc cagcgcgaga tattttttca tcattccggg tgcgtgttgt gtttgttttt
     7501 atgcaagctg ccggaggcgt gctccggcac tgtttttatt ttttatcttc cggcgtatag
     7561 ccctcgatat gcacctcttt accctcgaac aggaagttga ccatctcctg ctccagcagc
     7621 ttgcggtgct cggcattcat catgttgagt ttcttttcat taatcagcat ggtttgtttg
     7681 tgctgccact gcgcccaggc ttctttggag atctcgttat agatgcgttt tcccagctcg
     7741 ccggggtaca gctgaaaatc ctgaccttct gcttcacgtt gcaggaaagt acaaaaaatc
     7801 gttctgctca taaatcatcc tctttatcga ctcacgcgct aaaccggcgc gccagtgcgt
     7861 aactgctgta acaaacgctc cacgggagcc gccaggccaa ctgacggcgg ttgcgctaag
     7921 ttataccaga gcgcattgcc ttcatccatg cagccggtga atgacgacac gggaagccac
     7981 ataggcacaa tatctaagtg gaaatggctg aaggtatggc gaaacgcggt cagttgcgtc
     8041 aggttatcgg cagcaatctg ccgttgtgcc agccaatgcc gcaaactttc ttcgtcggca
     8101 aactgcggga aacagtataa accgccccac aatccgctcg gcggacgctg cgccagcaat
     8161 acttcatctt cgtgctgtaa caggagaaag tagccggtgc gctccggcag cgtctgtttc
     8221 ggttttttgc ccggataaag cgaccagcta ttgttggtgg cggcaataca tccgttttgt
     8281 agcggacaga gcgaacattt cggcttcgaa cgcgtgcaaa tcatcgcgcc caaatccatc
     8341 atcgcctgat taaaccgttc cacgccgacc gcgggcgtca cctgctcgct caaactccat
     8401 agtttattct cgacctcttt tttcccaggc cagccgctta cagcatagca gcgcgccagc
     8461 acgcgtttga cgttaccgtc gagaatcgga aagtgcttac ccagagaaag cgagagaatc
     8521 gcgcctgcgg tggaacgccc gacgcccggt aacgccgcga cttcttcaaa ggtttccggg
     8581 aatttaccac cgtgtaaggt cgccacttgt tgtgccgctt tatgcaggtt gcgcgcgcgg
     8641 gcgtaatagc caagcccggt ccacaagtgg agaacttcgt cgagcggcgc attggcgaga
     8701 tcggtcaccg tcgggaagtg cgccataaag cgttcaaaat aggggataac ggtcgcaacc
     8761 tgagtttgtt gcaacatcac ttctgagagc catactttgt agggcgtctt gtcaatttgc
     8821 cagggcagcg ttttccgccc gtatttatcg taccagtcca gaacctgggc tgaaaattgc
     8881 gacgcttgca tggtcaccga cttcactgtt gttgggggca agattgcagc acagcgacgg
     8941 cagggtgtaa accggaactt tccgcagcca cggccttatc atgcttgcat ccggcaacta
     9001 actttggata atgcccgttt tcagaacact ttcacaagcg actaaatctt tatgaaaaac
     9061 gacgtcattt caccggaatt tgatgaaaac ggccgcccac tgcgccgtat ccgtagtttt
     9121 gtgcgccgcc aggggcgact gaccaaaggc caggaacatg cgctggaaaa ctactggccg
     9181 gtgatgggcg ttgagttcag tgaagatatg ctggatttcc ccgcgctttt tggccgtgaa
     9241 gcgccggtga cgcttgagat tggttttggc atgggggcgt cgctggtggc aatggctaaa
     9301 gatcgccccg agcaggactt cctcggcatt gaagtgcatt caccgggcgt tggtgcgtgc
     9361 ctggcttctg cgcatgaaga aggtttaagc aacctgcgcg tgatgtgtca cgatgcggtt
     9421 gaagtgctgc ataaaatgat tcctgacaat tcattgcgca tggtgcagct ctttttccct
     9481 gacccgtggc acaaagcgcg ccataataaa cgccgtatcg ttcaggtgcc gtttgccgaa
     9541 ctggtaaaaa gcaaactgca gctggggggc gtattccata tggcgaccga ctgggaacct
     9601 tatgcggaac atatgcttga agtgatgtct tctattgacg gttataaaaa cctgtcagag
     9661 agcaatgatt acgtaccgcg tccggcatca cgtccggtga cgaaatttga acaacgtggt
     9721 catcgtcttg gtcacggagt atgggactta atgttcgaga gggtgaaata atggcaaaga
     9781 accgtagccg tcgtctgcgt aaaaaaatgc acatcgacga attccaggaa ttaggatttt
     9841 cggtggcatg gcgattcccg gaaggtacat cggaagaaca gattgataaa atcgttgatg
     9901 actttattaa cgaggttatc gaaccgaaca aactggcctt tgacggcagc ggttatctgg
     9961 cctgggaagg tctgatctgc atgcaggaaa tcggcaaatg caccgaagaa catcaggcga
    10021 ttgtgcgtaa gtggctggaa gagtgcaaac tggatgaggt acgcaccagc gaacttttcg
    10081 acgtttggtg ggactaagaa agcatacggg cgatgacaaa tgcaaaactg cctgatgcgc
    10141 tacgcttatc aggcctacag agatgcacga tcgagtaggc gggataaggc gtttacgccg
    10201 catccggcat ggaaaacgcg tactttgtta tcaatctggg gccagcatat gctggcctga
    10261 tttgttcttg agggaagact atgatgcgca aaatgctgct ggcggcagca ctttcagtga
    10321 cggcaatgac cgctcacgcc gactaccagt gcagcgtcac gccgcgtgac gatgtgattg
    10381 tcagcccgca aaccgtgcag gtgaagggcg aaaacggcaa tctggtgatc acgccagacg
    10441 gcaacgtgat gtataacggt aagcaatatt ccctgaatgc cgcccagcgc gagcaggcga
    10501 aggattatca ggctgaacta cgtagcacgc tgccgtggat tgatgaaggc gcgaaaagcc
    10561 gcgtcgagaa agcccgtatt gcgctggata aaattatcgt tcaggagatg ggcgacagca
    10621 gcaaaatgcg cagccgtctg accaaacttg atgcgcagct taaagagcag atgaatcgca
    10681 ttatcgaaac gcgcagcgat ggcctgacgt tccactataa agccattgat caggttcgcg
    10741 ccgaaggcca gcaactagtg aatcaggcaa tgggcggaat tttacaggac agcattaatg
    10801 aaatgggcgc gaaagcggtg ctgaaaagcg gcggtaaccc attacagaat gtgctgggaa
    10861 gcttgggcgg tctgcaatcc tcaatccaaa ccgagtggaa aaagcaggaa aaagatttcc
    10921 agcagtttgg caaagatgtt tgtagccgcg ttgtgactct ggaagatagc cgcaaagccc
    10981 tggtcgggaa tttaaaataa tcctctattt taagacggca taatactttt ttatgccgtt
    11041 taattcttcg ttttgttacc tgcctctaac tttgtagatc tccaaaatat attcacgttg
    11101 taaattgttt aacgtcaaat ttcccatgca aagctaaggg ataatgcgta gcgtccacgt
    11161 aactggagga atgaaatgga gtttttcaaa aagacggcac ttgccgcact ggttatgggt
    11221 tttagtggtg cagcattggc attacccaat atcaccattt tagcaaccgg cgggaccatt
    11281 gccggtggtg gtgactccgc aaccaaatct aactacacag cgggtaaagt tggcgtagaa
    11341 aatctggtta atgcggtgcc gcaactaaaa gacattgcga acgttaaagg cgagcaggta
    11401 gtgaatatcg gctcccagga catgaacgat aatgtctggc tgacactggc gaaaaaaatt
    11461 aacgccgact gcgataaaac cgacggcttc gtcattaccc acggtaccga cacgatggaa
    11521 gaaactgctt acttcctcga cctgacggtg aaatgcgaca aaccggtggt gatggtcggc
    11581 gcaatgcgcc cgtccacgtc catgagcgca gacggtccat tcaacctgta taacgcggta
    11641 gtgaccgcag ctgataaagc ctccgctaat cgtggcgtgc tggtggtgat gaacgacacc
    11701 gtactggacg gtcgcgatgt caccaaaacc aacaccaccg acgtagcgac cttcaagtct
    11761 gttaactacg gtcctctggg atacattcac aacggtaaga ttgattacca acgtaccccg
    11821 gcacgtaagc acaccagcga tacgccattc gatgtctcta agctgaatga gctgccgaaa
    11881 gtcggcatcg tttataacta cgctaacgca tccgatcttc cggctaaagc actggtagat
    11941 gcgggctatg atggcatcgt tagcgctggt gtgggtaatg gtaacctgta taaatccgtg
    12001 ttcgacaccc tggcaaccgc cgcgaaaaac ggcactgcag tagtgcgttc ttcccgcgta
    12061 ccgacgggtg ctaccactca ggatgctgaa gtggatgatg cgaaatacgg cttcgtcgcc
    12121 tctggcacgc tgaacccgca aaaagcgcgc gtcctgctgc agctggctct gacgcaaacc
    12181 aaagatccgc agcagatcca gcagattttc aatcagtact aatcacctcg tcccggcatc
    12241 gtgccggggc tttttcactt cagacttacg tccattgcca attttcatta ccctaatgat
    12301 aatcaccgga ataaattatt ccgcgcgagg gttttcgggt gaaaaagcaa tggattgttg
    12361 gcacggcgct gcttatgttg atgactggta atgcctgggc agatggcgaa ccgccaactg
    12421 aaaatatctt aaaagatcaa ttcaaaaagc agtatcacgg cattctcaag cttgatgcca
    12481 tcagcttaaa aaatcttgat gctaagggta atcaggccac ctggtcagcg gaaggcgatg
    12541 tctcttccag tgacgatctc tatacctggg tcggtcagtt ggcagattac gaactgctcg
    12601 aacagacctg gacgaaagat aaaccggtaa aattctcggc gatgttaacc agtaaaggaa
    12661 cgcccgcgtc tggctggtcg gtgaactttt actcttttca ggcggcagcc agcgatcgtg
    12721 ggcgggtggt cgacgatatc aaaacgaata ataaatatct gatcgtgaat agcgaagatt
    12781 tcaattatcg ctttagtcag cttgaatctg cgttgaatac ccagaagaac tcgattcctg
    12841 cgctggaaaa ggaagtgaaa gcgcttgata agcaaatggt ggcggcgcag aaagcagcgg
    12901 atgcatattg gggaaaagat gcaaacggta agcaaatgac ccgcgaagaa gcctttaaaa
    12961 aaattcacca acagcgtgat gagtttaata aacagaacga tagcgaggcg ttcgcggtta
    13021 aatatgacaa agaggtttat caaccggcga ttgcggcatg tcataaacag agtgaagagt
    13081 gttatgaagt gccgattcag cagaaacgag atttcgatat caacgaacaa cggcgacaga
    13141 cttttctgca atcacaaaaa ctcagccgta aattgcagga tgactgggta acgctcgaaa
    13201 aagggcaata tccgttaacg atgaaagttt cagaaataaa cagtaaaaaa gtcgcgattt
    13261 tgatgaaaat tgacgatatc aaccaagcta atgaacgcta gaaaaaagat accgaacaac
    13321 tgcggcgcaa tggggtgatt aagtaactca tgttaagtgc ctttgccgga taatataaaa
    13381 agttatccgg caaattattt tttacattag ccctagtagc tgtggaatga acaaggatat
    13441 ttgcggaatg taagtgatca tcatcaacgc tactagcaat gcagcataaa atagcagcaa
    13501 tggtttgatt aaatgttgaa tcttcacacc cgagatagaa caaccaacaa atagcgcact
    13561 acccaccggc ggtgttaaca gcccaataca aaggttggcc accatcataa taccgaaatg
    13621 tactgggtcc attcccagct cttgtgcaat cggcaagaag atcggtgtaa agattagtac
    13681 agccggtgtc atgtccataa agatgccgac aattaataag acgatattaa tcagcagcaa
    13741 tatgatcagt gggttatcag aaatccccat caatgcgtcg ctgatcatat aaggaatatc
    13801 agcattagtc attgcccagg acatgcccac ggaaaaaccg atcaacagta atacaatcga
    13861 agtggtaaca accgactcca gaatcagttt gggtaaatgg cgccatttta cttcccggta
    13921 aatcagcacc gagagaataa atgtgtagac aacggcaatt gctgacgctt cagttgcggt
    13981 aaaaatgccg cccagaatgc cgcccatcac aatgaacacc agaagcaaac ttggtaaggc
    14041 atccagaaac gctttacagg cctgagcaaa ggttgctctt tctgaaagcg ggtagccacg
    14101 gcgtttagca ataatgccgc agactatcat taccgcaaga cccattaaga tccctggcaa
    14161 atatcccgcc ataaacagcg atgctacaga aacgccgcca gctgtcaggg aaaacacaat
    14221 taacacgttt gagggcggta tcagtaatcc agtaatacaa gatgagacgt ttacagcggt
    14281 agaaaatgcc ggatcataac cttctttggt ctgaatggga ttcagtgtgc caccaacggc
    14341 agcagctgcc gcaacggctg aaccagaaat ggaaccaaac atcatattcg ccaggacgtt
    14401 cacatgacca agcgaaccag gaacgcggcc aaccattacc tgggcgaggt taatcaaacg
    14461 tatcgcaata ccgccgctgt tcatcagtgt tccggcaaag atgaaaaagg ggattgccaa
    14521 cagggcaaag ttatccagtc cattagccag tcgttgcgaa acagtgattg ccgcgatatc
    14581 ccagggaaac atcaaaagca ttgaagctac agtggcaata ccgattgaga acgaaatggg
    14641 cacaccaata aagaccagca caaaaaaaca gccaaacagt gtaatcgcga tataactatc
//
LOCUS       DAZOYF010000098        14635 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZOYF010000098 DAZOYF010000000
VERSION     DAZOYF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.098"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1213)
                     /locus_tag="ACHUOF_002753"
     CDS             complement(<1..1213)
                     /locus_tag="ACHUOF_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HFR0611871.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRRE
                     HAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALS
                     VIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKP
                     NSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYA
                     TLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIG
                     QALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRY
                     TPALLEVLELRA"
     gene            complement(1217..1777)
                     /locus_tag="ACHUOF_002754"
     CDS             complement(1217..1777)
                     /locus_tag="ACHUOF_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0611872.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQ
                     LEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATA
                     GEPKAQLAREFNISRETLYQYLRTDD"
     gene            2087..3664
                     /locus_tag="ACHUOF_002755"
     CDS             2087..3664
                     /locus_tag="ACHUOF_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HFR0611873.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFT
                     EEELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPI
                     AKNGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSII
                     SSAMAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSL
                     YTGRTDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQ
                     AETDQIATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGE
                     IRQATQVIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVAD
                     EVRSLAARTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAATALDGI
                     GQRVNEITDMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQ
                     LTSALSELAKQFWEKRG"
     gene            complement(3815..4252)
                     /locus_tag="ACHUOF_002756"
                     /pseudo
     CDS             complement(3815..4252)
                     /locus_tag="ACHUOF_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            4843..5094
                     /locus_tag="ACHUOF_002757"
     CDS             4843..5094
                     /locus_tag="ACHUOF_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFR0611874.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(5125..5370)
                     /locus_tag="ACHUOF_002758"
     CDS             complement(5125..5370)
                     /locus_tag="ACHUOF_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611875.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKQGALEV
                     AREIACRLRDIGWPLERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(5370..6296)
                     /locus_tag="ACHUOF_002759"
     CDS             complement(5370..6296)
                     /locus_tag="ACHUOF_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR0611876.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGALEKAL
                     AIACQLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            complement(6293..6682)
                     /locus_tag="ACHUOF_002760"
     CDS             complement(6293..6682)
                     /locus_tag="ACHUOF_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFR0611877.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
     gene            complement(6859..7125)
                     /locus_tag="ACHUOF_002761"
     CDS             complement(6859..7125)
                     /locus_tag="ACHUOF_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611878.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(7218..7652)
                     /locus_tag="ACHUOF_002762"
     CDS             complement(7218..7652)
                     /locus_tag="ACHUOF_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFR0611879.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            complement(8385..8885)
                     /locus_tag="ACHUOF_002763"
     CDS             complement(8385..8885)
                     /locus_tag="ACHUOF_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFR0611880.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDETDPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(9347..9943)
                     /locus_tag="ACHUOF_002764"
     CDS             complement(9347..9943)
                     /locus_tag="ACHUOF_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611881.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            complement(9940..10659)
                     /locus_tag="ACHUOF_002765"
     CDS             complement(9940..10659)
                     /locus_tag="ACHUOF_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFR0611882.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            complement(10656..11090)
                     /gene="psiB"
                     /locus_tag="ACHUOF_002766"
     CDS             complement(10656..11090)
                     /gene="psiB"
                     /locus_tag="ACHUOF_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR0611883.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWVMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(11145..13103)
                     /locus_tag="ACHUOF_002767"
     CDS             complement(11145..13103)
                     /locus_tag="ACHUOF_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR0611884.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETALSALLAQTEEVSVPLDSLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDARAYRNLPEPEAVLTEAED
                     ERLNELMARYDSLENQCEESDLLAAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CIQRGVQLRSEDDVADDADRTEQGLESASVEEISLPLLTKLTSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSKAHSSPARIRLECEHYALTSDAPSGKEGAAFMALMAEHSRF
                     AALLPEGWERDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCAPRPQTDATEHTANLADAA"
     gene            complement(13162..13395)
                     /locus_tag="ACHUOF_002768"
     CDS             complement(13162..13395)
                     /locus_tag="ACHUOF_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFR0611885.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNRYIRDFGILK"
     gene            complement(13453..13980)
                     /locus_tag="ACHUOF_002769"
     CDS             complement(13453..13980)
                     /locus_tag="ACHUOF_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR0611886.1"
                     /translation="MSARGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            complement(14364..>14635)
                     /locus_tag="ACHUOF_002770"
     CDS             complement(14364..>14635)
                     /locus_tag="ACHUOF_002770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611887.1"
                     /translation="QKVVYGCMQGTGVIFRDTVTVSGCLLLRPLRLPAAWRHPVVRPD
                     SDRQLLAHPPVSAEPAGAVSGCRYGPMRENPACSDRSGRAAPVAP"
BASE COUNT         3183 a         4283 c         4020 g         3149 t
ORIGIN      
        1 cggcgcgcag ttccagcacc tccagcaagg ccggcgtgta acggcgcagg gtggcgaagt
       61 tctcgccgac caggtgcagg tggtcgaagc cttccggccg ggccagcagc tcggcctcgc
      121 tgacgctctc ggtgaactcg tcccagggaa tcaccgcctc gatggcggca taggggtcgc
      181 tgccgctttc cttcgcttcc agcagcgcct ggccgatcct ggagtacagg cgcaccttgt
      241 cgttgatcgc cttgccctgc ttctggaact gctgctgatg cttgtgcttc gcgccgctga
      301 acagcttgac caggatgcgg tcatgcagat cgaccagctc atcgatcacg gtcgcggtgc
      361 tctccagcac cacggcggcc agggtcgcgt agcggcgctg cggctcgaac ttaccgaggt
      421 ctttgggcgt catctgccca ccctcgcggg ccagcttgag caggcggttc tggtggatgt
      481 gccggcccag gccttcgggc aagtccacca actgaaatgt cttcagccgc tcgatgtgtt
      541 ccagcatgtg ccgagagttg ggtttcagcg gtgcctggcg cagccaggtc aaccaggtga
      601 tgctgctgcc ggccttgagc ttcaacagct cgtccagctt ggcccgatgc gagtccgtga
      661 gtggttcgac cagggcgcgg tagacccgcc gattggctcg cgcaatggct tccgagcagg
      721 cccggtcaat cacgctcagc gccggcagga tgcgtcgttt ctgccgtagg ctctccaggg
      781 cctgaccggc cagcagcaag cctttgtcgg tctgctgggc cagctcggtt agctcgcgca
      841 ccagggcgcg gaagtcggac aggccgaacg gggccagttg caggtaggtg cgcagttcct
      901 gggcatgctc gcgacgggtc acgtcgcgct cgccgtactt tgcccagctc gccggctcgg
      961 cctggacttg cttcgccacc cacaggatga ccggctcggg cagctcgctg tcggttccca
     1021 gcgcgtaacc ggggtagcgc agcaggcaga gctgcacggc gaagccgagg cggttggcgt
     1081 cgccgcgtcg ctggcggatc agcgacaggt cggagtcgtt gaaggtgtag tagcggatca
     1141 ggtcatcctg gctttccggc agcgcaagca gggtgtcccg ctccgtggcc gagaggatca
     1201 agcgacgcgg catgtgtcag tcgtccgtgc ggaggtactg gtagagggtt tcccggctga
     1261 tgttgaactc gcgggcaagc tgcgccttgg gctcgccggc cgtcgctcgc tgccgcaggg
     1321 tagcagcctg ctcatcggac agggctttct tgcggccccg gtacgcgcca cgctgcttgg
     1381 ccaaggcgat gccctcacgc tgccgctcgc ggatcagggc gcgctcgaac tcagcgaagg
     1441 cccccatcac cgacagcatc aggttggcca tcggcgagtc ctcgccagtg aacaccaggc
     1501 cctccttcag gaactcgatg cgcacgccgc gctgagtcag cttctgtacc aagcgacgca
     1561 ggtcatcgag gttgcgggcc agccgatcca tgctgtgcac caccactgta tcgccttcgc
     1621 ggacgaagct cagcagcgct tcgagctggg ggcgctgggt gtccttgccc gatgccttgt
     1681 cggtgaacac cttgctcacc tgggtttgtt ccagctggcg ttccgggttc tggtcaaagc
     1741 tgctgacccg gacgtagccg atgcggtgcc cctgcacgat gtctccttgg ttgaaggcgg
     1801 cttaagtgca ctttctgttc cgttgtgcct caaagcccat ttctgtcagg ctgaaatcta
     1861 taaccttcgc gggcatgtgt caaaaaatgg gaaagcagac tctattctga cgaagcggcg
     1921 cggccctgcc tgacatcaag ttagggtata gcctagattg acatgcgcga tgcaaccctt
     1981 aacttgcttg cacctatcgt ttccatgcta gctttatcgt aacgctcaag aaattgggct
     2041 taatgccgaa aacaataaaa taaaacagcc aacccttgag gttcttatgc gccagaattt
     2101 accagtgacg ggtcgaaact tagaactccc aaaagatgcc aatattcttt cgactacctc
     2161 ccctcaaagc catatcacgt acgttaatcc tgacttcatt aaaatcagtg gtttcactga
     2221 ggaagaacta ttaggccagc ctcacaacat cgtaagacac ccagatatgc cgcctgctgc
     2281 atttgagcat atgtggagta cattaaaatc tggccgctca tggatggggc tagtaaaaaa
     2341 tcgctgtaaa aatggcgacc actattgggt aagtgcttat gtaacgccaa tagctaagaa
     2401 tggttcgatt gttgaatacc agtctgtaag gaccaagcct gaacctgagc aggttttggc
     2461 tgcggaaaaa ttatatgctc aattgagaag cgggaaggcc gcgaggccga aattggctgc
     2521 tagcttttcc gtgaaaatac tcttgctcat atggggtagt attatatcaa gcgcaatggc
     2581 tgccggcatg cttactgata catcaataag cagcttattg ttagccactt taatgtcagg
     2641 aagcttaagc tctgttagtg ttttggctat tctctctcct cttggaagac tggttgaaag
     2701 agccaggaat atttccaata acccattaag tcaatccctc tacactgggc gcaccgatga
     2761 gtttggccaa atagagtttg ctttacgaat gatgcaagct gaaacaggcg ccatagtagg
     2821 tcgcataggt gatgcatcaa atcggcttag cgaacacacc cgaggcctac taaaggatat
     2881 tgagtcaagc aatgtactta cagttgagca gcaggcagag acagatcaaa tagcaacggc
     2941 agtaaaccaa atggtggcaa gcattcaaga ggttgcgagc aatgcacagc atgctgcaga
     3001 tgcggccgga agagcagaca ctgagacggc atctggccag cgtctggtag cccacacaag
     3061 ccagtcaatc actgcccttg aaggtgaaat taggcaagcc actcaggtta ttcatgagct
     3121 tgaaggtcaa agtaacgaga tatcaaaagt tcttgacgtt atacgaggga tcgccgagca
     3181 aacgaatttg ttggcactca acgcagcaat tgaggccgcg cgtgctggtg agcaggggcg
     3241 tggttttgct gttgtcgccg atgaggttcg cagtcttgct gctcgcacac agcaatcgac
     3301 aacggatatt caaagcatga tcagcgctct acaagagcga gcgcaatccg ctgttacagt
     3361 catggagcaa agtagtcggc aagcgcacac gagtgtagct cacgcagagg aagcagctac
     3421 agctcttgat ggaattggcc aacgcgttaa cgaaattacc gacatgaacg cgcaaatagc
     3481 gactgcggtc gagcagcagg gagcagtaag tgaagacata aaccgcagta ttatcaatat
     3541 acgcgatgct gctgatacca atgtacagac cgggcagaat aatttgcaaa gtgcgaaatc
     3601 tgtcgctcag ttaactagcg ctctgagcga actggcaaaa cagttttggg aaaaacgagg
     3661 ataacgcttt tcagtatctc gacagggata acttacttta ccatcggtta tcccttttag
     3721 ccgcacaaat tttagccatg gcttttcagg aagtcagggc tatcagaatg gccttagaaa
     3781 gcctagtcaa agagctgtca cgagaacacc gttagcttag cgtacgattt tttccgaatt
     3841 ctgcggttcc ccccagtaca ttgtccaggg tgatttcagg acaggtgtcg ctcatcagct
     3901 ggctgttcca ctcatcacgc tggcatgaac tcatgaaggt gtacatgccc gtatctgtca
     3961 tcaggcggtc ccagattttc cggtcggcaa gacgggtgat attccttttc gcttcttccg
     4021 tcttatccat gcgacgaacg gaatcagtca cgcagtcttc aaacccgtaa agaaaaatat
     4081 cacctgacgc atcgagcaga atcttgcgcg ctgtcgccag gcattccatc gcctgcatga
     4141 acagtgcaat accttcgtta cgctgcgcga tgatgcggtc gatagctaca gacggaataa
     4201 catctgtcgt gccggcagta ttcaggtcag cagtgggtaa agtctgattc atcacattgt
     4261 ctcctcatcg tcacgactgc cgggtcagcc aggtcatttc ccggaggagc gaagctcctt
     4321 ctgccgtccg ggtcttcggg gatttccccg cagtggacct ttccgacggg ggacgctcac
     4381 cacgtcacgg ggcagttgtc cgggggagag atgtaccagc cacaaaagtt tttgacgcct
     4441 gcggcaaaaa gttttgtggc tggtacatct ctcccctgga cgactgaaac gggatgtggt
     4501 catgtatgta cccccggaaa gtccgtctgc ggagaacagg ccgcagtgcg gcggcagaca
     4561 ggcagagaca gctgcgccat caggcgcagt ggccttacac accgtaaggt gtgcagggag
     4621 cgccgcatac ggcgcgactg aagaaaaacg acggcacaac aatggcatgt ggtggtcact
     4681 ggcacaccac tcccgcacag gtgaaaacag agaaaaccgc acgacattta caaccaaaaa
     4741 aaagtgaaat ttcttcactt tttcacgctt atttttcgcg ttgatttggt gattatgatg
     4801 ataaaataaa gtgaaatttt ttcacttttt caaaggtgtt ttatgaccgg ctgggaactg
     4861 cgaatctggc gaaaaagcat gctctggtca cgcgaaaaag cggccagaga atttggcgtg
     4921 acgcagagaa catggcacgc ctgggaaaat gcggaacagg tggacgtcac cgtctggagg
     4981 accacccagg cactttcagt ccgtgacctg ctgcctcaca tgcaggggat gcgtaaggca
     5041 gatatcatca ggcgactgga aaatgaactg ggggaaacgg cagggaatgt gtgagcgtga
     5101 ttcacagcct ggctgaatgc caggctactg ctcatcagga aacagctttt tcagttcttc
     5161 accggaaagt cccgtgacct cctgaatccg ttccagcggc cagcctatat cccgaagccg
     5221 gcaggctatt tcccgcgcca cttcaagagc cccttgttta aaaccctcgt cgaaaccttt
     5281 ctgtataaga cgttctgcaa tcgtcatgct gtttgctctg ctcctgctga caaaaaagcc
     5341 cggcattttg ccgggctttg tggttctcat cagtgagtga ttttcttcag ttcgtcatcg
     5401 gaaagtccgg tagcctgctt aatctgctcc ggcgtcatcc ccattttctg aagctggcac
     5461 gcaatagcca gggctttttc aagcgctcct tccatgcgtc cttccatgcg tccttccatc
     5521 ctgccttcct ggcgaccttt ttgctcaaga cgttctgcga tagtcatcag tttatcctca
     5581 tgctgcggca gacgctcagc cagcgcccgc ataaattccg gtgatacaga gtcccctgcg
     5641 ttaacgatgt aatgaatcag aacacgcacc tgctcgtctg aaatttccac catgacctgc
     5701 gggagtttgt ccagcagctc catcatgtca cgatggcgga tatgcttcat cagcagcgtc
     5761 agtgcggcca tccggcgatg gttcatgatt tcattgtcat caacgacagt gatatcaacc
     5821 agcggaaacg gctttgtgta tattttagcc gcaagtgccg gattctcaaa acagtccagc
     5881 cagttcatgc tgtacgggta cgggctgcgc tcaccgtgat agaacagcac cggaatcacc
     5941 agtggcaacg ttttgtgctg ctccagatgt ctctgcatcg ccgccacact gtagcgtaac
     6001 atgcgaaaag gcatgtacag attttctgtg ctctggtgct cgatgagcgt ataaacataa
     6061 ccatcttcac ccccggtggt tttcacgctc cagaggatat cgctggcgta ctgatgcagg
     6121 tccggctcaa caaaggtggc gggttccagc ttcagcgtgg acaggtcgca caactgccgg
     6181 tactccgcag gaagatgcag ttccagaaaa tccctggcaa cgtcgggatt cgccaggaac
     6241 gaccggaacg cggcatcatg cggcgtgggc gttgtggtgt ttttcttctt actcacggtt
     6301 cctccatcaa atcagcagca tcgcagcaat catcgcccaa atccccacaa ccgagaggac
     6361 agcaattgcg ttcaaagcca tgacaacaat agccgcgttc tgagtcacgg cgtatttctt
     6421 cagcaatctc ttctgcggaa aggtaatcaa actcctcacg ggccagttcc aggaccagta
     6481 gatgttgttc tgtcggcaat gtggccacaa aattatcaat agcagcactc aaacgtcgct
     6541 gatatagctc ataaacaaga ccacaacggg gcattagttc ttcggataat agttccagtt
     6601 ttgagcgaat tctgtcagcg ttgaaagtga cttcatcaaa agacatatga aaagctttgt
     6661 cagtatcaaa acaagacttc attgttatct ccagccctcc cgggccctga atgaaagaga
     6721 atctgtttcc cttttctgtt ttttgtggag acaccatgca acacagacgt cgtgatgttt
     6781 ttcatcttgc tcacggttcc tcctggtaaa gtacctgaac acatcctgcc acctgcacag
     6841 gcagacggca ggggttaatc agtgacagac ccgttcaatt tcaagcgcgc ggagcaggtg
     6901 gccccattcg ctgactttca tggcggggat acgtacccgc ttctgctcac tgcagcgtct
     6961 ggcgatatga ccgcccatcg ccaggacgtc cgggttaatc tcatccggtg ccagttcctg
     7021 gcgtctgaag cgggtatggc ggtgcagggg tttgttctcc cgcaggcact cttcacgcag
     7081 acgctttaca tactcggcgg ttgcgaagat aattgatggt ctcatggcat aatctccata
     7141 gttagtgaat gagatgccgg acttccgtac ggggaagcgc cagcggagaa aggcagggtt
     7201 tatcgccctg ctttttttca ttccggacag cgacgcagtg ccagcgcgag cgctttcatc
     7261 ttccagcctt ccggcgtgcg gtacatcacc ttccagtagc cgtcgatgaa cacataatgg
     7321 aagcgcacct cttcagtgct ttccgtggcg atggcctccc tgaaagaacg gtactcacgc
     7381 gcctcactgt ccatccggaa accactgtca cgtccgtaat aagccgtcac accgtccacc
     7441 gggttctgaa gcgtatgccc ggcgggtttg tcgcaacggg gaccgaggca acggatatca
     7501 ccgtgttcaa ccagggcttc ggcctgctga ccggtggcat agtgggtatg caggatgcga
     7561 ccgttccagg tcggctcacc gtgcaggtgg cagtaaacgg tacggaacac cccgtcgcgg
     7621 caaaaaacgg aaatcgtgct gctgactgtc atgaaaacgt ctccttctgc tgaatcaggt
     7681 cgccgggccg accggttgct cccccggagg ggcgaaagcc ccttctgccg tccacttctt
     7741 cggggtttct ccccgcagtg gacctttccg atggggcagc gactgccacg gcacggggca
     7801 gttgtccggg ggcagacgca aaagtttttg acgcttgcgg caaaaagttt tgtggctggt
     7861 acatctctcc cctggacggc tgaaacgtgt tgtggccgct tcagcccccg gaaagtccac
     7921 agcggggaaa caggccgcag ggggggcggc ggaagggcag acaactgcgc catcaggcgc
     7981 agtttcctta cacaccgtca ggtgtgcagg gagcgcaggt cacggcgcga cagaggcggc
     8041 gcgaccggaa cggtcgctgc aagcctggtt tttcagacat cggaccgtag cggcagcccg
     8101 aaaccgcgcc agcgggttcg gcggagactg gcgggtgcgc cagcagctgc ctgtcggagt
     8161 cgggcctgac gaccggatgc cgccaggcag ccgggagccg aagggtgtcc ccgcccccgg
     8221 gagtcagaac aacagaaacg acaaaacaga aaaatgcctc acactatgac aggcaggaag
     8281 ggtagcgccg caggcgcggc cctcctgtat ttcaggcagg cgtaagcctg ccagggtttt
     8341 ccgggggcct ggcaggccat ccccccgccg gagcggggag taaatcaccg acggaacaca
     8401 tgaccgtcaa taaaggtgaa ggagtccagg aataaatccc gcgcatacgc ctcatagtca
     8461 aaatagagcg ccaccgtctc cggcacgtca gccagcaggc cggtatcact gacgaactca
     8521 accgcaaaag cctcctcact gtcagcctcg ccccaccagg catcgcggaa gctgtcaaaa
     8581 tccgtctcac cggtatcatc cacccagaga cgataagcct cttcgcagcc ttcatcccgc
     8641 gcctggcgga agccttcaac ccaggcccag ttgatatggc attcagaggc catattcccc
     8701 gggaatccct cataatcctg aaacatcagt tccgggtcgg tttcatcctg gtgaagggca
     8761 cggcaggcgg cgaaaaagtc gcgttcatca tcaaacgttg tcaggtcaaa ccagcggcca
     8821 gcgatgcttc cacagttgta tttgtgccag gttccaacgt atacagcagg tgcaacaaca
     8881 gacattcccg aatcctccat agtcacatgt tctgcgacac cgggcggtgc cgtgcctcca
     8941 tctttgccgg gcctgcaggg atgcaagggc gcaagggaag ccgcagcccg acgcacacgg
     9001 ctggcgggag ttggagggtg agaccgcgaa atctgagcgg gtgaacgacc ggaaaatcac
     9061 ggcagacggg aaggagggac cgggtgaccg cagcggttca tcttaccttg catcccgccc
     9121 gcagaagccc ggctacgatg gaggcacggc acagcccgca cacgacacgg cggccacata
     9181 actgaaaacc cacaaggcca tgccccgcat ggccgggtaa aagcggagcc ggcggcgacg
     9241 tccttattcc ggcgagagca gggagcacac cgcatgatct ccagaccacc cctggaacga
     9301 aacgatcatg accggcatgt ccctgcgtaa aatatacatg cctggttcac cggagaatat
     9361 ctgtgatatc cacaagtttt ctgtcgcgca gccagtcctg cgcttcacgc accacacaac
     9421 tgtccggttt caccgctttc agcgatgatg ccagtcgctt gatcaacatc accagttccc
     9481 gtgtctgtat gtccggcatt gtctccggcg gagcaggggg actctcattt ccgacgctca
     9541 tcccatgcgc ttccagtgtg gcacggacaa accaggcggc atcgaccagt tgctgtccaa
     9601 tccaggccag atcggcattt ctgtcacggg ctgccattcg ctcgcccgac agcgccttgc
     9661 tgatatggct gcgcaccaga ttatccagga gtgcctgcgc ttcttccggt gaaaagccac
     9721 ctggtatctc tgtgcgcaac agccctttcc tgtacagaag ctgcagcaga tcaacctgcg
     9781 accgcacagc aactatcagt acataacgga caacattcgc actggcagtg gtttctgtat
     9841 cattcagcag ttcttccgga tcgttccccc cggtgttgca gagcaacggc aacagaggtc
     9901 gcatccacgc gggaacctgg tatttttcgt ctgatttcat catattacgt tctccagctt
     9961 gttcgggatc atccactgct caagtgcacg tacctgtacc ggtgcaccac gaccggcctc
    10021 accggcttca aaaatcagcc gccagagtgg ttcctcctgc cagaaaaacc ggtcaaatga
    10081 tgacagtgat ggaaaacgcc cgcaccaccc ccagaaaatc gtttccagat catgctcctc
    10141 aacaacacca gaccagcggg acaaccagct tccgaccgtg gcgggagtat gaaacgcaag
    10201 gtcaatctgc gcctgagcaa aacatgtctg gtattccagt tcccgtcgtt ctgcctcacg
    10261 ttcccgctgg cggctgtatt tctgctctgt cagcgtggtt ttctgacgac gacgctcacc
    10321 ttcacgaaaa ggcacggacg ggaacagacg gtgccccgca tcggatggca ggggaagaac
    10381 acagacttcc ccaaaacttt caatcagttt atccacggca gccagccaca ggagacggtt
    10441 gccatgaaat tcctccttcg tcagcgcagg ggcaaaaaag cggagatcgg tcagcgaaat
    10501 ccggcgactg ccattcaggc agcgaaagaa agcggtggca taaggccatg ctgaaagtgt
    10561 gcgtccctga cgacgacgtt gttctgtgac agccaccgcc tgcatggcgg cctgttgttc
    10621 tgcgctgagg gggatcagtg cccgtgaacg tgcgctcatg cctgacctcc ttctgccatc
    10681 aggatatgga tgatgtcgtt aacactgtat ccctgcgcat ccagtgtcgc ggcaagcgcc
    10741 agcgtgtgac tgatcgcgac aggcgtgaag atatgttgca cctgaacgac ggcgaaaggc
    10801 tgtccaccac cattcaccag aaccagcatc cagagctgag agacgtctcc cggcgaacat
    10861 aatgccaggt ggaaacgttc gtgggctggc gtgaaacgaa cctggacggg gaaaaagccc
    10921 ccgaactcac tgccatactc tccgttcatc acccagttag ccggtgcgtc cacttcacgc
    10981 atcacctcgt gagtgagatt acggcgcata tcgcttcccg cagcacggat atcttcatat
    11041 tcctgtgcgt tcatggactg cagggcattc agggtcagtt cagttttcat cacggtatct
    11101 cccttgctgc cgccccgtct ccggcggggc ggtgtggttg ttcatcaggc ggcatcagcc
    11161 aggttagcgg tgtgttcagt ggcatccgtc tgtggacgtg gtgcacacat ccagccaggc
    11221 acccagcggt tgtctttcat atggaactct gcatgttcag ccgcatcgcc tttcttcatt
    11281 ttctccgcgt cccgtgcggc accggacagt ccggcctcat tcagggctgc gataatctgc
    11341 ggctttttca ggtgtccgaa gaagtttgct tttgtcggct gccaccagtc gcgcatgtga
    11401 aagccgatgg cggtttccag cgagtccagc ggactgcgtg acgtgtgacc acactcgcgg
    11461 gtctggacac cgttaagact gcacgcggta cagaaactga gcagggataa cagcacctcc
    11521 tggctaagtg acaggaacgt cgtcatatcc cgttcccatc cttccggcag cagggcagca
    11581 aaacgagagt gttctgccat cagcgccatg aatgcggcac cttccttccc tgacggtgca
    11641 tcgctggtca gcgcataatg ctcacattcc aggcgaattc tggcgggact actatgcgct
    11701 ttgctgtcaa acacattcag gcagagcgtc catgccagca gtgccaggga tttgtccggt
    11761 tgctgcatca gtgctgcctg gactgccagc gtgcgctctg aggtcaattt agtcagcaac
    11821 ggcagactaa tttcctccac tgacgcgctc tccagcccct gttccgtgcg gtcagcgtcg
    11881 tcagccacat catcctcact gcgcaactgc acaccacgct ggatacacac attaccgtaa
    11941 cgccaggaca ccaccacgcc acttccggca cgcatctccg gcgtccacgc cctgaccttc
    12001 gccatgcagt caatcagctt catttctgct gccagcaggt cagattcctc acactggttt
    12061 tccagcgagt cgtaacgagc catcagttcg ttcagacgtt cgtcttccgc ctccgtcagc
    12121 accgcttccg gctccggcag gttgcggtat gcccgggcat cttcacggca ctcaccgaca
    12181 ggctccatgc gtccggcgca ccattcccag ccttcggctt cccgaaggaa ctctgcgaca
    12241 gcctggagtt tttccagcag ggcggcatcg agcgccacgc ggtcgacata accgtccccg
    12301 tcatcgctga acaaatcggt gcgcagttcg tctggcgaga aggcatcagc ccccacgaag
    12361 cggaatttac tgttccccgc caccgccact tcactttcgg tcaccagacg acgaatggtc
    12421 tgtacttccg gtttaccgcc ccatcccgac tggcaggcgg cttcaaacac ctgcacctga
    12481 cgcgcggtgt cgttctccag cgccagcgcc tgacagtgct cggtggtgat gcggtcttct
    12541 gccagcgcat cgaggataac cggagcaagg tcagccagtt tcagcattcg ctgaacgtgg
    12601 cggggtgaat agcccagcaa atcaccgatt tgtgcaggtg ttttgccttc ctgcgccatt
    12661 gcgcggaatc cggcaatctg ttcggcaggg tgcatatccc gacgctgacc gttctcggtc
    12721 atcgatgcgg cagtcgccag ctcctgcgga atgaccttca cgcgtacagg ccagtcagcc
    12781 ggaaggatgt tgcgctctgc cagcatgttg agtgctgcca gtcggcgacc gcctgcggcg
    12841 acaccgtaac ggtcacctgg cagggcatga acaaccagat tctgcagcag gccgactccc
    12901 ttaatggaat cagccagttc actgacggac tccgcagaat acggcaccgt gcgcacattc
    12961 agcggtgatt taatcagtga atccagcggc acgctcactt cctccgtctg cgccagcagg
    13021 gcagacagtg cagtttcctg cgtttttaca gatttacggc ttgctttgcg ggtggtttct
    13081 ggttcacact tcgttacagg cataatcatc cccttataat tgcttatgtc ctgccggacg
    13141 ttcgcgcgtc cggcacctct cttacttaag aatcccgaaa tccctgatgt accggttcat
    13201 ccagtacccg gcaccgtcct caaaattcca ggagcgccag atcaacgtgc catcctgacg
    13261 gacaaccagg cgaaaatgcg ttccctgatc atcctcgata aagacgttcc ggtactgtgc
    13321 ggcaacggct tccgcctgtt cgcgggtaaa ggagccgtcc ggcagtccct gaagtattct
    13381 gaaatattcg ctcatcctca tatctccggt gatgccccgc acagggcggg gcggttgtca
    13441 cagtcagccc gatcagaacg ggatgtcgtc tgaaaacccg taatcctccc cctccggcgg
    13501 ttgcggctga ggctctggct gcgcggcctt acgtccacgg cctttcgttt tcgcgccacc
    13561 ttttttcgtc gcgtcagcac tctgtggctg cccattctgc tgaggcttcg gttgcgcctg
    13621 agcgttctgc tgtggtgcac gtcccagcat ctgcacggtg cccgtggtct taacaagaat
    13681 ttcagtgacg taacgggtga taccgttatc ttcccagcta cgggtgcgaa gctgaccttc
    13741 gatgtagacc tgcgcaccct tgcgcagata ttcacctgcc acttccgcga gcttgccgaa
    13801 caacaccacg cgatgccatt ctgtctgctc ccgcatctcc cccgtctgtt tgtcacgcca
    13861 gctttctgac gtggccacct gcaggtttgc cactgcgccc ccgtttggga tgtaacggac
    13921 ttccggatct tttcccagac gcccgacgag gatgaccttg ttgataccac gtgcactcat
    13981 aagaacctcc tgactgaaag atgtgagata aaccggatac cggcgacggg acgttcatgt
    14041 cccccttcag aggagcgaaa gctcctcctg ccgtccactt cacagggttt tctccccgca
    14101 gtggaccttt ccgatggggg cccgtttacc gcctcacggg acagctgtcc gggggcggac
    14161 gcaaaatttt ttgccgggtt catggctaaa aattttgtgg ccggtgcatc tcaccctgga
    14221 cagatggaac gggagggggt tacgtctgcc ccccggaaag tccacagcgg gaaacaggcc
    14281 gcagaggtgg cggcaggagg gaagactgct gcgccatcag gcgcagtttc ctcacacacc
    14341 gtcaggtgtg cagggagcgc aggtcacggc gcgacagggg cggcgcgacc ggaacggtcg
    14401 ctgcaagccg ggttttcacg catcggaccg tagcggcagc ccgaaaccgc gccagcgggt
    14461 tcggcggaga ctggcgggtg cgccagcagc tgcctgtcgg agtcgggcct gacgaccgga
    14521 tgccgccagg cagccgggag ccgaagtggg cgcaataaaa gacagccaga aacggtgaca
    14581 gtgtcacgga atatcacacc tgttccctgc atacagccat acactacctt ctgac
//
LOCUS       DAZOYF010000099        14481 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZOYF010000099 DAZOYF010000000
VERSION     DAZOYF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.099"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..118
                     /locus_tag="ACHUOF_002771"
     CDS             <1..118
                     /locus_tag="ACHUOF_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611888.1"
                     /translation="NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(115..381)
                     /locus_tag="ACHUOF_002772"
     CDS             complement(115..381)
                     /locus_tag="ACHUOF_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR0611889.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            655..4179
                     /gene="nifJ"
                     /locus_tag="ACHUOF_002773"
     CDS             655..4179
                     /gene="nifJ"
                     /locus_tag="ACHUOF_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR0611890.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4546..5675
                     /gene="ompN"
                     /locus_tag="ACHUOF_002774"
                     /pseudo
     CDS             4546..5675
                     /gene="ompN"
                     /locus_tag="ACHUOF_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5816..6250
                     /gene="uspF"
                     /locus_tag="ACHUOF_002775"
     CDS             5816..6250
                     /gene="uspF"
                     /locus_tag="ACHUOF_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR0611891.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6426..7361
                     /gene="ttcA"
                     /locus_tag="ACHUOF_002776"
     CDS             6426..7361
                     /gene="ttcA"
                     /locus_tag="ACHUOF_002776"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR0611892.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="ACHUOF_002777"
     CDS             complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="ACHUOF_002777"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR0611893.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(8887..9060)
                     /gene="ynaL"
                     /locus_tag="ACHUOF_002778"
     CDS             complement(8887..9060)
                     /gene="ynaL"
                     /locus_tag="ACHUOF_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR0611894.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="ACHUOF_002779"
     CDS             complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="ACHUOF_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR0611895.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(10332..10379)
                     /gene="ydaN"
                     /locus_tag="ACHUOF_002780"
     CDS             complement(10332..10379)
                     /gene="ydaN"
                     /locus_tag="ACHUOF_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR0611896.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            10573..11805
                     /gene="dgcM"
                     /locus_tag="ACHUOF_002781"
                     /pseudo
     CDS             10573..11805
                     /gene="dgcM"
                     /locus_tag="ACHUOF_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="ACHUOF_002782"
                     /pseudo
     CDS             complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="ACHUOF_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12643..14097)
                     /locus_tag="ACHUOF_002783"
     CDS             complement(12643..14097)
                     /locus_tag="ACHUOF_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFR0611897.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14094..>14481)
                     /locus_tag="ACHUOF_002784"
     CDS             complement(14094..>14481)
                     /locus_tag="ACHUOF_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR0611898.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3861 a         3651 c         3418 g         3551 t
ORIGIN      
        1 taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt
       61 aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata
      121 gctgccacag ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc
      181 acacatccca atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat
      241 tgcacaattt gcttctccac tactggacat cgacgctttt aaacttggcg ctacgtgcgc
      301 cgcagtcgcc tgctgaacag gctcactact acacgccgac aacaataaag cggcacaccc
      361 tacccaaaac gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca
      421 taaataatag gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt
      481 ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat tactctgatg
      541 tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta
      601 ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt
      661 actattgacg gtaatggcgc ggttgcttcg gtgacgtttc gcatcagtga agttatcgcc
      721 atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac
      781 ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt
      841 gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca
      901 tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca
      961 tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactttctat ttttggcgat
     1021 cattccgacg ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc
     1081 caggaagcgc aagactttgc tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca
     1141 tttattcatt tctttgatgg ttttcgcacg tcccacgaaa tcaataaaat tgtcccgctg
     1201 gccgatgaca ctattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg
     1261 gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc
     1321 cagtctcgcg aagccaccaa tccctggtac aacgcggtct atgaccatgt tgaacaggcg
     1381 atgaatgatt tcgctgccgc gacaggtcgt cagtatcagc cgtttgagta ttacgggcat
     1441 ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg
     1501 gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg tctgtaccgt
     1561 cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtacgcag cgtggcggta
     1621 ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc
     1681 gcactggcag aagcctttaa taatggtgag cgcgaaaccc tgccccgtgt cattggtggg
     1741 cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag
     1801 ctcaacgcgg ctaaacctaa agcacgcttt acggttggta tttacgatga tgtgaccaat
     1861 ctgtcactgc cgttgccgga aaacactctg ccaaactcgg cgaaacttga agccttgttt
     1921 tatggccttg gtagtgatgg cagcgtttcc gcaaccaaaa acaatatcaa gattatcggt
     1981 aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa agcgggcggc
     2041 ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc gttccgctta tctcatttcc
     2101 caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag
     2161 cgtttaaaac ctagcggcat tttcctgctt aatacgccgt acagcgcaga tgaagtgtgg
     2221 tcgcgcttgc cgcaggaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg
     2281 attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg
     2341 cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg
     2401 cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg atctggtgga gcgcaactgg
     2461 caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg
     2521 cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta
     2581 accgcggcga tgctcgccgg gcttggtgat gccctccccg tttcggcgct gccgccagac
     2641 ggcacctggc cgatgggcac cactcgctgg gaaaaacgca atatcgccga agagatcccc
     2701 atctggaaag aggaactctg tacccaatgt aaccactgcg tcgccgcttg cccacactca
     2761 gctattcgcg caaaagtggt accgcctgaa gcgatggaaa acgcccctgc cagcctgcat
     2821 tcgctagatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg
     2881 gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat
     2941 ccagagatta aagccatcaa tatgatgtct cgccaggaac atgtcgaaga agagaaaatc
     3001 aattacgatt tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat
     3061 attcgtacat cgcagctgat tacaccgctg tttgaatatt caggtgcttg ccccggttgt
     3121 ggcgagacgc cgtatattaa attgctgact cagctctatg gtgaccggat gttgatcgct
     3181 aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc
     3241 gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt
     3301 ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat
     3361 caatttgccg ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca
     3421 gaggttcgtc gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca
     3481 catgaacttc tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc
     3541 gacggctggg cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa
     3601 aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg
     3661 aaagcgacac cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt
     3721 aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc
     3781 ggcgcgcagc tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccgggacca
     3841 tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt acgatctggc tctcagccac
     3901 gaccagatgc gccaactcac tgctaccggc ttctggccgc tatatcgctt tgatccgcgt
     3961 cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg
     4021 gaagaaacgt tacttcacga gcagcgtttc cgtcgtctga attcgcagca gccagaagtg
     4081 gcagaacagt tatggaaaga tgctgcagct aatttgcaaa aacgctatga cttcctagca
     4141 caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc
     4201 caattctaaa aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat
     4261 cagtggcaat gcaatggccc aacagaaaat aaaatctgtg acaataaagg catataaccc
     4321 gcgcagaata acgtatagga aattaaaagt ataattttta ttttgtatgt attatcttcg
     4381 atacattcac cagacttatt atcatttcag aggaattatt tgattaaggt tttacttaag
     4441 gcgtaacaaa tgatatttat cggctaactg aacttctcct ttaggatgtt ttcactcccc
     4501 gaaatgggga taaaaggcaa ataaaataac taaaggattt attcaatgaa aagaaaagta
     4561 ctggcacttt taattcctgc cctgctcgcc gcaggtgctg cacatgcagc cgaagtttat
     4621 aataaagacg gcaacaaatt agatctgtat ggcaaagttg atggcctgca ttatttttct
     4681 gataattcag cgaaagatgg cgaccagagc tatgcgcgtc tgggttttaa aggcgaaacc
     4741 caaattaacg atcaacttac tggctacggt caatgggaat acaatattca ggcaaacaac
     4801 actgaatctt caaaaaacca gtcatggacc cgtctggcat ttgccggact gaaatttgca
     4861 gattacggtt ctttcgatta cggacgtaat tatggcgtaa tgtacgacat cgaaggctgg
     4921 accgatatgc tgcctgaatt tggcggtgac tcttatacca atgcagacaa ctttatgact
     4981 ggtcgagcca atggcgtcgc gacttatcgt aatactgatt tcttcggtct ggtaaatggt
     5041 ctgaacttcg cggtgcagta tcaaggtaac aacgaaggag ccagtaatgg tcaggaaggc
     5101 accaataacg aacgcgatgt tcgtcatgaa aacggtgacg gctggggtct ttccacaaca
     5161 tatgatttag gcatgggctt tagcgctggt gcggcataca cctcttctga ccgcaccaat
     5221 gaccaggtta accatactgc ggcgggtggt gataaagcag acgcgtggac tgctgggcta
     5281 aaatacgatg ctaacaatat ttacctagca accatgtatt cagaaacgcg taatatgacc
     5341 ccgtttggcg acagcgatta tgcagttgcg aacaaaaccc agaactttga agttactagt
     5401 accagtttga tttttgtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc
     5461 ttcacgctgc tggtggtgca gacaacccgg caggtgttga tgataaagat ctggttaaat
     5521 acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacctat gttgactata
     5581 aaatcaacct gttggatgaa gatgacagtt tctacgctgc caacggcatc tctaccgatg
     5641 atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg
     5701 gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccgtcattac
     5761 acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa
     5821 cagaacgatt cttgtcccta tcgatatttc agattcagaa ttaactcaac gcgtgattag
     5881 ccacgttgag gcagaggcaa agattgatga tgcagaggtt catttcctga cggtaatacc
     5941 ttcactgccc tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga
     6001 tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc
     6061 aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaatt
     6121 ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac
     6181 ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt
     6241 tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt
     6301 gctgacattt tcccctctaa tccgtaccat acacgccaca gtttttatat cagatttctt
     6361 atgctgagcg ttccggcatg acgcatactc ttctgatgcc atataacgaa ttgagtcgct
     6421 tttaaatgtc gcaaaatcaa gaaattagta agaaagaaca atacaacctg aacaaattac
     6481 aaaaacgtct gcgtcgtaac gtgggcgaag ccattgctga cttcaatatg attgaagaag
     6541 gcgatcgcat catggtttgc ctctccgggg gtaaagacag ctataccatg ctggagattc
     6601 tgcgcaattt gcagcaaagc gcgccaatca atttttcgct ggtggctgtt aacctcgatc
     6661 aaaagcaacc gggcttcccg gaacacgttc tgcccgagta tcttgaaaag ctgggcgttg
     6721 agtacaagat tgttgaagag aacacttacg gtatcgtgaa agagaagatt ccagagggca
     6781 aaaccacttg ctcactgtgt tctcggctac gtcgcggtat cctttatcgc accgcaacgg
     6841 aactggggac gacgaagatc gcgttgggtc accatcgtga cgatatcctg caaacgttgt
     6901 tcttaaatat gttctacggc ggcaagatga aaggtatgcc tccgaaactg atgagcgatg
     6961 atggcaaaca tatcgttatt cgtccactgg cctactgccg cgagaaagat attcagcgat
     7021 ttgccgatgc aaaagcgttc ccgattattc cgtgcaacct gtgcggttca cagcctaacc
     7081 tgcaacgtca ggtgattgct gacatgttgc gtgactggga taaacgttat ccagggcgta
     7141 tcgagacgat gttcagcgcg atgcagaatg tggtgccgtc gcatctgtgc gataccaacc
     7201 tgttcgattt caaaggcatt acccacggtt ctgaagtggt taacgggggt gatctggcgt
     7261 ttgatcgcga agagatccca ctacaaccgg cgggctggca gccagaagaa gatgaaaatc
     7321 agttggatga gttacggctg aatgtggttg aagtgaaata accaggatag cgcccgatgc
     7381 gcaagcgtat cgggctactc ttatggaggc cggataagac gcggccagcg tcgcatctgg
     7441 caatcccgaa taagatgttt actcttgcac ccggcaattc aacatttcat tattttaata
     7501 accgcacccg gcacgttttt cctttaatct tcccgccctg taactgtttc catgctttat
     7561 gagcaacagc ctgacggacc gcgacataga catgcgccgg atgcacggcg attttgccaa
     7621 tatctgcgcc atcaagcccg atatctcctg tcagtgcacc taatacatca cccgggcgca
     7681 ttttggcttt tttcccgcca tcgatacaca acgttgccat ttctgcttcc agcgtcacaa
     7741 tggaactatt agctggcggc gtttgccagt taagttttat ctgcaacatg tcagaaatga
     7801 tattggcccg ctgtgcttct tccggagcac agaaactgat cgccagaccg ctatttcctg
     7861 cacgagctgt acgaccgatg cgatgtacat gaacttcagg gtcccacgcc agctcaaagt
     7921 tcaccaccag ctcaagcgat ttaatatcca gaccacgcgc agcaacatca gtcgcaacca
     7981 gtacacgggc gctaccgtta gcaaaacgta ccagggtctg atcgcgttgc tccagatcgc
     8041 cgtgtaacga caatgcactt tgctctactt cattcagcgc gtcgcagaca gcctggcaat
     8101 cttttttggt attgcaaaac accacacaag aggatggctg atgcaagctt aataaccgtt
     8161 gcaacagagg aattttgcct ttactggatg tctcataaaa ttgttgttca atgggtggca
     8221 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgattg
     8281 cggcgatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     8341 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     8401 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     8461 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg cagcgaatca cgctgcatac
     8521 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     8581 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     8641 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     8701 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     8761 cggcggcctg caccggcgtc atggttaaat aacccaactc attaaggttc gtgagttggg
     8821 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     8881 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     8941 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     9001 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     9061 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     9121 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     9181 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     9241 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     9301 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     9361 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     9421 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     9481 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     9541 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     9601 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     9661 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     9721 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     9781 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     9841 cactggaatg atcggtcaac gcatcgcaca catccaccag ccatcccccg caatcggtcg
     9901 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     9961 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
    10021 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgaaacggc
    10081 tgactcgggg ccgtgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
    10141 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
    10201 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
    10261 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccactg
    10321 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
    10381 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
    10441 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
    10501 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
    10561 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
    10621 tggattgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
    10681 atgcaagacc tcacgtttag tcagctacga accggaccct attccgtctc ctcacaaaaa
    10741 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
    10801 gttcagcgta aagaagagga aacagcatta agctgtcggc ttgttttgcg agagctgaca
    10861 gaaacagaac cggtgattat tttcgaaggt atcgaagcgc cggcaacgct gggtttaaaa
    10921 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
    10981 actaactctg caccaatgtt gttgattgac ccgtcacgag atggacaaat cgtcgatgct
    11041 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
    11101 taggaaataa atatgcttgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
    11161 cccggcggtc ataacccttt gaattttgtt cataaactgg cggatggatc tactcgtcat
    11221 gtgcagacct atgccggacc gattgaaatt tatggcgaca agctcatgtt atgtattgtg
    11281 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc tcaccatgac
    11341 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttacggaacc aggccaaatg
    11401 cagcatctcg ccatcgctca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
    11461 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
    11521 cccctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
    11581 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
    11641 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
    11701 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
    11761 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
    11821 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
    11881 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc ggtgcaatat
    11941 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
    12001 tgtgatttat cttcccgccc tttaccatga ataatcagca cattacgcaa accatccgcc
    12061 agggcttgtt gaatgaaact gaacaccatt ttgcggcact cttccaccgg ctggcgcaaa
    12121 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgc
    12181 tacaaccctt cctgccgaaa ctccagcggc tgacttagcg ggatgatgtc gagaaatccg
    12241 gtagtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
    12301 gttggatgcc agtggacatc tgtagcacgt ttcagcggct ggacatcttc catggcgtca
    12361 agaaacagcg atttgtcgtc aaggttcatg taacctccta tgcaccttca cacgtcgtga
    12421 ttcagacggg ttaattgttc atacttactg catcgttccc ttcacagatt gaaacaatac
    12481 tctacttcat acctgtgagt atctgcgcag gcggggcatt ttatactagc ctgttagcag
    12541 tatcgaacag tttagcccga gtttaattca gcacaggctc cgatcaggat gatttattca
    12601 tagcaggaac caacaaaaat gcgataaata acagaccact gattagcaga aaggtaccga
    12661 aaatgccata gtttgcgcct ccctcagcat caattgactc aataatttgg ttgaagccag
    12721 tatttccgat gtctggtcac ttctactgcg attaaaaaat aaagcacttc tggaaatgaa
    12781 gtcattcacc gtatggggaa cagaaaccat tttttcaccc attctcgaaa aatgaaaatt
    12841 agttcgatta ttgagaatag agccacacag ggcaatacca atcgcgcccc caagattacg
    12901 tgttaaatta aatacaccag aggctaactt cagccgttct tttggaattc cccccagcgt
    12961 taatgttacg atgggggcca tcgcaaactg ctgcgaaatc cctctaattg cctgtggaaa
    13021 cagtaattcc tgccatcccc attcgtgggt tataggggta aaaagataca ttgaaaacac
    13081 aaatccgccc agccccgcca tcaacagcca tcgcagattt atttttttac ttaaccagaa
    13141 gtaaaaggga acagaaaata actggaaaat acctgttgtg caaacggcaa aacctatctc
    13201 ttcggcattt aatccgcgaa tctgccccaa aaacacaggt attaaatata cagtagagaa
    13261 tatcccaacc cctccagaaa aagaaaaaaa acatcccaga gtgaaatttt tatccttaaa
    13321 agcatgaaga tccattatcg gattggatat tgtaagtgta cgcgcagcaa ataaaataaa
    13381 actcaccaat gccagaactg atgtcagcaa aatagtatta tcatccagcc atccccagcg
    13441 cgctccttct tcaagggtat attccagaca tcccagagtc atggccaaca ggataatact
    13501 cggataatca gcaactttca gtaaagagag atcaggttta tcaaaattaa ccagaaaagg
    13561 aatactcaaa actaaatata tgccaggtaa tatattgata taaaatagcc atcgccagtc
    13621 gaggttatca gtgagccagc cccccagagt tggccctagc gtcggtgaca gtgaggccag
    13681 cgcactgact accgcagcag ccaaccccaa ctctttaccc tggtaataaa taaacgccat
    13741 cgtgaaaact aaagggatca tggatgcgcc agcagctccc tgtaaagcac gaaacaaaat
    13801 catgatctga atattccatg caagtccaca cgcgatactc attaaagtaa atattcccgc
    13861 ggatagagta aaaagccaac gagtggaaaa tacccgtgac agccacccag agagcggaat
    13921 aacaataatc tcagcaatta aatagctggt ctgaagccag gccatttcgt ctttacctgc
    13981 ggacagacca ccacctattt catttaggga tgacgataca atttgaatat caagctgagc
    14041 aataaataat cccatacaca tactggcgaa agcaatatgt ttacgcatag acatcatctc
    14101 tcatcaacac tgacggtgac ggacagtccc gggcgaagaa gttgctttaa ttcctttgcg
    14161 tcgtcaaatg caatccgtac cgggacacgc tgaacaattt tggtgaaatt gccagtggca
    14221 ttttctatcg gaatcaagct gaaactggct cctgtcgctg gggaaaggct ttcaatgtga
    14281 ccgtgaaaca cttccccttt gagaatatca gcacggatct cagccttcat tcctggcttc
    14341 attcccgcta tttggttttc tttataattt gcatcaaccc acaactcact gactggtact
    14401 agtgaaacta acgatgtgcc gccttccacc caactgcctg tatgggcgct gcggtttgcg
    14461 acaatgccat caatcggcgc a
//
LOCUS       DAZOYF010000100        14430 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZOYF010000100 DAZOYF010000000
VERSION     DAZOYF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.100"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            229..336
                     /locus_tag="ACHUOF_002785"
     CDS             229..336
                     /locus_tag="ACHUOF_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0611899.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            712..819
                     /locus_tag="ACHUOF_002786"
     CDS             712..819
                     /locus_tag="ACHUOF_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0611900.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(906..2585)
                     /gene="bcsG"
                     /locus_tag="ACHUOF_002787"
     CDS             complement(906..2585)
                     /gene="bcsG"
                     /locus_tag="ACHUOF_002787"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR0611901.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2582..2773)
                     /gene="bcsF"
                     /locus_tag="ACHUOF_002788"
     CDS             complement(2582..2773)
                     /gene="bcsF"
                     /locus_tag="ACHUOF_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR0611902.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2770..4341)
                     /gene="bcsE"
                     /locus_tag="ACHUOF_002789"
     CDS             complement(2770..4341)
                     /gene="bcsE"
                     /locus_tag="ACHUOF_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR0611903.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4614..4802
                     /gene="bcsR"
                     /locus_tag="ACHUOF_002790"
     CDS             4614..4802
                     /gene="bcsR"
                     /locus_tag="ACHUOF_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR0611904.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4814..5566
                     /gene="bcsQ"
                     /locus_tag="ACHUOF_002791"
     CDS             4814..5566
                     /gene="bcsQ"
                     /locus_tag="ACHUOF_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR0611905.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            5563..8180
                     /gene="bcsA"
                     /locus_tag="ACHUOF_002792"
                     /pseudo
     CDS             5563..8180
                     /gene="bcsA"
                     /locus_tag="ACHUOF_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            8191..10530
                     /gene="bcsB"
                     /locus_tag="ACHUOF_002793"
     CDS             8191..10530
                     /gene="bcsB"
                     /locus_tag="ACHUOF_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR0611906.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGTPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            10537..11643
                     /gene="bcsZ"
                     /locus_tag="ACHUOF_002794"
     CDS             10537..11643
                     /gene="bcsZ"
                     /locus_tag="ACHUOF_002794"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR0611907.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11838..12227
                     /locus_tag="ACHUOF_002795"
     CDS             11838..12227
                     /locus_tag="ACHUOF_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HFR0611908.1"
                     /translation="MISNAACKTTVWHSRQRYWKTRANGRRRQHFSGNDWRWAPAAYG
                     LLTDWQEEAARQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQVKDYAPSHFL
                     LYVRYSAAGWQGDMDLPPQPLIPYADW"
     gene            12309..14297
                     /gene="hmsP"
                     /locus_tag="ACHUOF_002796"
     CDS             12309..14297
                     /gene="hmsP"
                     /locus_tag="ACHUOF_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR0611909.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
BASE COUNT         3401 a         3671 c         3957 g         3401 t
ORIGIN      
        1 gtgacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aagggttccg
       61 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acatcacggg ggccattctt gaacctgaac
      181 aactcaagaa tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg tcatgcgggc gtcaggctgc
      361 cgtaatggca atttgcgccc ggaccaggcc gcaggggtga aactctgcgg cctttttcgt
      421 aagaaaaatg caacgattgc acgcggataa tattctgcgg aatgcgcagc acagagtgaa
      481 gacgtgacgg attccggaac agaaaaacag tcgtagaatc agcggcaagc cggaaaggtt
      541 ccggtgaggc gcaatgttgc gggggcttta tccctggtgg cattggttgc tggagagaga
      601 aaacccccgc acgttgcagg tatgcacctg acaacatcac gggggctaat cttgaaccca
      661 aacaactcaa gaatagccgc gaaacgttgg cattacaaca caggcggcta tatgacgctc
      721 gcagaactgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc tggcattctt
      781 gccagtatga tcgtgaactg gctgaataag cggaagtaac gtgtcatgcg ggcgtcaggc
      841 tgccgtaatg gcaatttgcg cccggatcag gccgcagagg ttaaactctg cggccttttt
      901 cgtgcttact gcgggtaagg cacccagtcg ccgccgttca ggcgaacgta cggtttatct
      961 tggtattgaa taactactgc atttgagttc tcggagaccg gtgctgtttg tggcaaccca
     1021 ctggtgagtt ttttccagtc aacattgtct tcggtgaaaa tcttgccatc aagaacgcga
     1081 accaccagat cggagatagc caggaagctg ctcggttggt caatgacaat cggtgccccc
     1141 tgatgtggtg ccttcatgcc gaagaatttc accccaacgg ggacgtctgt gatagacggg
     1201 ctagggatat cacgtaggcc agatacctgc attctgtcgc ccttcagcgc gccgccgtgt
     1261 tccggcacca cgaccaccat cactttacga cccgatttct ccagttcagt aaagaaggcg
     1321 tccagttcat caaagaattt ctgcgcccgc gctttgtaat ccgctgtttt gctgaccccc
     1381 ggataatggt tgccgtcatg cagtggaagc gtgttgtaga acgtggcact acggctgttt
     1441 ttatcttttt cggtaacgtc cagccagcgg ttaagcacgg cggtatcgtc ataaaccggc
     1501 gaaccatcaa agcccagcaa aataaccggc agatttgttt gatccatcaa ttcagtctgc
     1561 atgccgccat tttcgcgaac ttctttcaaa aaaccgccga actggccgtt atgtcccatc
     1621 atcaggtgct gggtaaagcc cagtttcgaa aggttatcaa acagatagca gtcgttattt
     1681 gccggttgat acagattagt gtgcgaagtc tgcccgcagc tggcgcgcag taaacggatc
     1741 gccgccgggc cactgtagga ggtggcggag ttaaagttct tgaaatcaat atcgaaatgc
     1801 gaccacagtg gatgcgacat caacccggcg gcttctatat ccgaccagga aagcgaacag
     1861 atgttaatca ccagtagttc aaatggctga gcatcagcgg gcagcgaaga cgggaaggtc
     1921 gatttacgtt tcgcctccgc gttatagaaa ttattcagcc aggcgttaag gttcgccgtt
     1981 gttggcggtg cagtttgtgc gggcatatca cccactaccg gtgcgccacc cgtcgccgca
     2041 acggttgccg ctgcgttacc acccgtcgtt gttacagtgg tcgtcggttg tccggctggc
     2101 cacaaggaga aacttggtcc cgccagggta agtacgttca gccatagcag tatggcaacc
     2161 acaaaaacgg taatgcgaat ccattgtgac aggaataacc aggccactaa taaaacaaaa
     2221 atggccccaa tcatctgcca gttaataaag cgtgtgacaa ggtcgattaa ataatcggta
     2281 ctgaaccccg ccacctgcga accctggctc attatgcttt ccgggccagg caaccagatg
     2341 tcatgccaga acaaagcaaa gccgatcggc agggcaatcc agtggcgcaa gcgatgcagg
     2401 ctgtaacgcg gaatgggcat cagcagaaac gcggcaaaca ccaaattgag gagcgggtgg
     2461 aagttaagat atcccgccca caacaggcca aacttaacca gaaaatagaa gttccagccg
     2521 gaaaggccgc gccagtattg ccagagggaa gaaggcatgg cggtattttg cgtaaattga
     2581 gtcatttttt ggttgccctg gctttttccg tgcggcgtaa cgtcccggcc ggtttaacat
     2641 aacgaggttt agcaaagaac aaacgtaagg tgtcgcgaat gcgtcgcaaa gagtgccgcg
     2701 ccagatagcc cagcgggaaa aatatcagtg cgcaaacgat aataatttca atgatatcgc
     2761 tgatggtcat catgatgagc gctccacagc atcatctaac agtcgcatgg gttctggtat
     2821 tcgtcgccag tggcgaccat cgtgctcggc attgatgacc ggtttagaac tttgcgttaa
     2881 aggcagcggc attccccatt gttctggggc aagcaggcgc atctgcacca gctcggcact
     2941 gatttgatca tcttcaaacc agaccatacg gtttgagaaa atgtcgccag taggcaatgg
     3001 gaaaatatga ttcaacgcgg tatccagatc gttaatccga cagaatgaga gaaacagcac
     3061 cagccgatta ccgccaatgg tcatgatatc gccggtacgg ttagggcgac atagcgtcag
     3121 ggcttgctca acgcggatac ccggtaccgg acgtagggca accagaacgc ctttaccgtg
     3181 ggcaggcaag agagggttat tcatcatgtt gttgacggca ttacagaaca catcccactt
     3241 ctggaaacca cgcagtttga gcggctgggt cattgacagc aaggtagtga tatcttccgg
     3301 tacatagcga ctaaacttct gcccttgcac gctttcgatc atcgtcagac aacgggagag
     3361 tggcgcattc cacggaataa ccatatttgc accgcaggcc aataacaaac gttcatcggt
     3421 ggcgcgcagg ctagcggtat tttcccgcac gaggattttc atcgcactac cgcgctggcg
     3481 acgcagggta tgaatgctgc gggccagtgg ctcgatttgc gcattttgct gtaaagaaaa
     3541 gaccaccgtc gccgcctgag cggtacgggc ttcattgaac aggacttcgt tattgttgaa
     3601 cagttgccag tgttccgata gcggcggcgc accttccagt acagcaacat tactcagaat
     3661 gcgtttttcg tcgctgcgtg gttggatctc cgcctcttcg ctttgaacta atgtccagat
     3721 accattttgt tgctgaacgc taagctgctg acgggcgctg accccttttt cgttgcacca
     3781 gaaggcaata tccagcaaat gttggtcacc ctgaaaacgc aaactggcaa gaccaaaaag
     3841 tgaacggtat tcctcaagca acaatgaaaa ttgtttatcg ttattatttc cgggattaat
     3901 taccaaaagc gaacaatggt ttaacctgct ccatttattc attttatcca accatgagcg
     3961 aagccgctcg gcaggaatat tttgccatgc gttatttgcg caaacaagaa taaaaaggta
     4021 attatgggga tcaatagaac actgcaaatc acgggccaaa tagtatagac ctttttcatg
     4081 atttagcatt gaaaataatt ttattttttc cggcccttga gaatcatcta attgaaagat
     4141 tttcgccgga tcgctgtcca tgctaatgac cgcgacgtgt gcggtttcag cctgggatgc
     4201 aattgtttga ttcgccagac tgatagcatc ttcatggcga tcgacgttaa accaccagac
     4261 gccgcctgct ggcatatgtc gcagctcatc ccataatgat gagataccga tagagaatac
     4321 agggtccaca atgtccctca tgtcaccatt ttgtctgatt atcagtttac tagcgaaagc
     4381 taagaaataa acctaccatt gaaattaaaa cttatcagat ttagcatgtg aatcaattta
     4441 gaaggtcaca agacgtgtga ttttagtctt ttcgttttta actttttctg ttgcgtaacg
     4501 gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac aataaaccgg cgaaatgctc
     4561 acggttagaa ttgtctcatg aatggtacgg ttatttcata gggatcaagc aaaatgaata
     4621 acaatgaacc agatactctg cctgatcccg cgataggcta tatcttccag aatgatattg
     4681 tggcgttaaa gcaggcattt tcactgcctg atattgatta tgccgatatt tcccaacgcg
     4741 aacagttggc cgcggcatta aaacgctggc cgttgctggc agagtttgcg caacaaaagt
     4801 aggggattgg tgaatggccg tactgggatt gcagggggtg cggggaggcg tggggacaac
     4861 aaccatcacc gccgcattag cctggtcatt acaaatgttg ggagaaaatg tcctggtggt
     4921 cgatgcctgc ccggacaact tgttgcgcct gtcatttaat gttgatttta cccaccgtca
     4981 gggctgggcc agagcgatgc tggatggcca ggactggcgt gacgctgggt tgcgctacac
     5041 ctcgcagctc gatttgctgc cttttggtca gttatccatt gaagaacaag aaaatccaca
     5101 gcactggcaa acccggctga gcgatatttg ctccggctta cagcaactaa aagccagcgg
     5161 gcgttaccag tggattttaa tcgacttacc gcgtgatgcc tcgcagataa cccaccagct
     5221 gctgagtttg tgcgagcact cgctggcaat cgtcaatgtg gatgccaact gccatatccg
     5281 actgcatcag caagcgctgc cggatggcgc acatattttg attaatgact tccgtattgg
     5341 cagtcaggtt caggacgata tttaccagct ttggttgcaa agccagcgcc gattactgcc
     5401 gatgctcatt catcgtgatg aagcgatggc tgaatgcctg gcggctaagc aaccagtagg
     5461 tgaatatcgc agtgatgcgc tggcggctga agagatactg acgctggcga actggtgcct
     5521 gttgaactac tccgggctga aaacgccagt cgggagtgca tcatgagtat cctgacccgg
     5581 tggttgctta tcccgccggt caacgcgcgg cttatcgggc gttatcgcga ttatcgtcgt
     5641 cacggtgcgt cggctttcag cgcgacgctc ggctgtttct ggatgatcct ggcctggatt
     5701 tttattccgc tggagcaccc gcgctggcag cgtattcgcg cagaacataa aaacctgtat
     5761 acgcatatca acgcctcgcg tccgcgtccg ctggacccgg tccgttatct cattcaaaca
     5821 tgctggttat tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag cgggcatttt
     5881 caggtctgca aaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg
     5941 ttagccataa aacacagcat ctggatgaaa aaaaagagct cggtcatttg agtgccgggg
     6001 cgcggcggtt gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg
     6061 ttactcagcc gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggggtag
     6121 cgctgatcgt acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc
     6181 tgaccgtttc ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc
     6241 cggtcagcct ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc
     6301 tggtgctcgg ctacttccag gtagtatggc cgctgaatcg tcagccggtg ccattgccga
     6361 aggatatgtc gctgtggccg tcagtggata tctttgtccc gacttacaac gaagatctca
     6421 atgtggtgaa aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga
     6481 acatctggat cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg
     6541 gggtgaaata tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg
     6601 cgctgaaata tgccaaaggc gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc
     6661 gatcgttcct gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc
     6721 agactccaca ccacttcttc tcgccggacc cgtttgaacg caacctggga cgttttcgta
     6781 aaacgccgaa cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg
     6841 acgccacttt cttctgcggt tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg
     6901 gcggcattgc tgtcgaaacc gtgactgaag atgcgcatac ttctctgcgt ttgcaccgtc
     6961 gtggctatac ctccgcgtat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc
     7021 tgtcggcgca tatcggtcag cgtattcgct gggcgcgcgg gatggtataa atcttccgtc
     7081 tcgataaccc gctcaccggt aaaggactga agtttgctca gcggctgtgc tacgtcaacg
     7141 ccatgttcca cttcttgtcg ggcattccac ggctgatctt cctgactgcg ccgctggcgt
     7201 tcctgctgct tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc
     7261 tgccgcatat gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact
     7321 ctttctggag tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg
     7381 tggcgctgat taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg
     7441 aagaagagta cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctactcaacc
     7501 tggtgggcgt tgcggtaggc atctggcgct acttctatgg cccgccaacc gagatgctca
     7561 ccgtggtcgt cagtatggtg tgggtgttct acaacctgat tgttcttggc ggcgcagttg
     7621 cggtatcggt agaaagcaaa caggtacgcc gatcgaaccg cgtggagatg acgatgcccg
     7681 cggcaattgc ccgcgaagat ggtcacctct tctcgtgtac cgttcaggat ttctccgacg
     7741 gtggtttggg gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc
     7801 tgttgcttaa acgcggtcag caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg
     7861 gtaatgaagt tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc gattttgtgc
     7921 agtgtacgtt tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata
     7981 agccgctgga aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg
     8041 cggagtttgc gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct
     8101 gggttgtatc gtttattccg cgccgcccgg agcggagcga aacggcacaa ccatcggatc
     8161 aggctttggc tcaacaatga tgataacgcg atgaaaagaa aactattctg gatttgtgca
     8221 gtggctatgg ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa
     8281 ccactgatca atgctgagcc agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg
     8341 caagtgatgt caggcgagca gggagctgat gcccccatcg tggcgcagaa cggtccttcg
     8401 cgtgatgtga agctgacctt tgcgcaaatc gcgccgccgc cgggcagcat ggtgctacgt
     8461 ggtattaacc cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag
     8521 gcgatgctca acttcgaata cacgccatcg ccatcgttac tgcctgtcca gtcgcagtta
     8581 aaggtttatc tcaatgatga gctgatgggc gtgctgccag tgaccaaaga acagttgggt
     8641 aaaaaaacgc tggcgcaaat gcccattaat ccactgttta ttaccgactt caaccgtgtg
     8701 cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa acccggccag caccacgctt
     8761 tggctggatg ttgggcggag cagtggactg gatctgacct atcagaccct gaatgtgaag
     8821 aatgacctgt cacacttccc ggtgccattc tttgacccgc gcgataaccg tactaacacc
     8881 ttgccgatgg tctttgcggg tacgccggat gttgggctgc aacaagcatc tgccattgtc
     8941 gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga acttcccggt gctctataac
     9001 caattgccgg atcgcaatgc cattgtcttt gcaaccaacg acaaacggcc ggacttcctg
     9061 cgcgatcatc cggcggtaaa agccccggtg attgagatga ttaaccatcc gcagaatcct
     9121 tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag acctgttgca ggcagcgaaa
     9181 ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg tggtagtgaa tgaagtgaaa
     9241 ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg tacgtaccga tcgtccggtc
     9301 acatttggcg aactgaaaac ctatgaagaa cagttacaat ccagcggcct tgagccagca
     9361 gcgattaacg tttcgctaaa cctgccgccg gatctctacc tgatgcgcag taccggcatt
     9421 gatatggata ttaattaccg ctacaccatg ccgccggtga aagacagttc gcggatggat
     9481 atcagcctga ataaccagtt cctgcaatcc ttcaacctga gcagcaaaca ggaggcgaac
     9541 cgtctgctgc tgcgtattcc ggtattacaa ggtttgctgg atggcaaaac agatgtctct
     9601 attccggcgc tgaaactggg cgcgaccaac cagctgcgct tcgactttga gtatatgaac
     9661 ccgatgccag gcggttcggt agataactgt attaccttcc agccggtgca gaatcatgtg
     9721 gtgattggtg acgattccac catcgacttc tcgaagtatt accacttcat cccgatgccg
     9781 gatctacgcg cctttgctaa cgcgggcttc ccattcagcc ggatggcgga tctgtcgcaa
     9841 accatcaccg tgatgccgaa agcgcctaac gaagcacaga tggaaacgtt gctgaatact
     9901 gttggtttta tcggcgcaca gacgggcttc ccggcgatta atctgacggt gaccgatgat
     9961 ggcagcacca ttcagggcaa agatgccgac atcatgatca tcggtggtat cccggacaaa
    10021 ctgaaagacg ataagcagat cgacctattg gtgcaggcga ccgaaagctg ggtgaaaaca
    10081 ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg agagcgatcg cgcggcagaa
    10141 acccagtcaa cgctgacctc ttccggtgcg atggcggcgg tgattggctt ccagtcgccg
    10201 tataacgacc agcgcagcgt gattgcgctg ttggcagata gcccacgcgg ttatgaaatg
    10261 cttaacgatg cggtgaacga tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg
    10321 atccgcgagt ccggtatcaa cagcctacgt gttggcgacg tttattacgt aggtcatctg
    10381 ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg
    10441 gcgactatca gtgtgatatt gctggcatgg gtactgtggc gtctgctgcg aattattagt
    10501 cgtcgtcgtc ttaacccgga taacgagtaa ttgaagatga atgtgttgcg tagtggaatc
    10561 gtgacgatgc tgttgttggc tgcctttagt gttcaggcag cctgtacctg gcctgcctgg
    10621 gagcagttta aaaaggatta catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg
    10681 cgcaaaatca ccacctccga agggcaaagt tacggtatgt tctttgccct ggcggctaac
    10741 gaccgtgcag ctttcgataa tattctcgac tggacgcaga acaatctcgc tcagggttct
    10801 ttaaaagaac gtttgcccgc ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg
    10861 ctggacagca attcggcctc cgatggtgat gtctggatgg cctggtcgtt gctggaggcg
    10921 gggcgtttgt ggaaagagca gcgttatacc gacatcggca gcgcgttgct aaaacgtatc
    10981 gcgcgggagg aagtggtgac ggtgcctggg ctgggttcca tgttgttacc gggcaaagtg
    11041 ggttttgctg aggataacag ctggcgtttt aaccccagct acatgccgcc gacgctggcg
    11101 cagtatttca cccgctttgg cgcgccgtgg accacgctgc gcgaaaccaa tcaacattta
    11161 ttgctggaaa ccgccccgaa aggcttttcg ccagactggg tgcgctatga gaaagacaaa
    11221 ggctggcagc taaaagccga aaaaacattg atcagcagct acgacgctat ccgcgtttac
    11281 atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag cgcggatgct caaccggttt
    11341 aaaccgatgg cgacattcac tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct
    11401 acggggaaag cgcagggtaa aggaccggtc ggtttttctg ccgccatgct gcctttttta
    11461 caaaaccgcg atgcgcaggc agttcagcgc cagcgcgtgg ccgataactt tcccggcagc
    11521 gatacctatt acaactatgt gctgaccctg tttggacaag gctgggatca acaccgtttc
    11581 cgcttctcga caaaaggtga gttattacct gactggggcc aggaatgcgc aaattcacac
    11641 taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa
    11701 ccatcagcag accctgcgga tggacagcgg caacactaat gccgtgcgcg ggctggcgaa
    11761 tatttaccgc cagcagtcgc cagaaaaagc tgaagcgttt atcgcctcgc tctctgccag
    11821 ccagcggcgc agcattgatg atatcgaacg cagcctgcaa aacgaccgtc tggcacagca
    11881 ggcagaggta ctggaaaacc agggcaaatg ggcgcaggcg gcagcacttc agcggcaacg
    11941 actggcgctg ggccccggca gcgtatggat tacttaccga ctggcaggaa gaagctgcgc
    12001 ggcaatccaa cgatggcggc agcagtcagg gcttcggcta cacggcgcgg gcattacttg
    12061 aacgacgtgt tacttccaac tggtttgttg gcacggcaat tgatatccag caggtgaaag
    12121 attacgcacc cagccatttc ctgctctacg tacgttattc cgccgccgga tggcagggtg
    12181 acatggattt accgccgcag ccgctgatac cttacgccga ctggtaagtt ttcagatagc
    12241 gcctctctta atgccgctgc gatcgggtat actcgggcgg caatctggga tttccggggg
    12301 gagacaattt gcgcgtaagt cgctcgttaa caatcaagca gatggcaatg gtggcagccg
    12361 ttgtcctggt gttcgttttt attttttgca ccgttttgct gttccatctg gtccagcaga
    12421 atcgctataa cacggctacg caactggaaa gcattgctcg ctctgtccgc gaacccttat
    12481 cttcagctat tttgaaaggc gatattcccg aagcggaagc tattcttgcc agcattaaac
    12541 cggcaggcgt ggtcagccgt gccgatgtag tgctgcctaa ccagttccag gcgctgcgta
    12601 aaagttttat tccagagcgt ccggtgccgg taatggttac tcgcctgttt gagctaccgg
    12661 ttcaaatctc gctgggcgtc tactcgctcg aacgtccggc aaatccgcag ccaatagcct
    12721 atctggtgct acaggcggat tccttccgta tgtataagtt cgtgatgagc actctctcaa
    12781 cgttagtgac catttactta cttttgtcgc ttatcctgac cgtcgccatc agctggtgca
    12841 ttaaccgcct gattttgcat ccgttacgca atattgctcg cgaacttaac gccatcccag
    12901 cccaggagct tgttggtcac caactggcat taccgcgtct gcatcaggac gatgaaatcg
    12961 gtatgttggt gcgcagttac aacctcaacc agcaattgct gcagcgccat tatgaagaac
    13021 agaacgaaaa tgcgatgcgc ttcccggtgt cggatttgcc gaacaaagcc ttgctgatgg
    13081 agatgctgga gcaggttgtc gcgcgtaaac aaaccaccgc gctgatgatc atcacctgtg
    13141 aaaccctgcg tgatactgcg ggcgtgctga aagaggcgca acgagaaatt ctgctgctga
    13201 cgctggtgga aaaactcaaa tcggtactgt cgccacgtat gatcctcgcg cagattagcg
    13261 gttatgactt tgctgtcatt gccaacggtg tacaggaacc gtggcacgca atcactttgg
    13321 gtcagcaagt actcactatc atgagcgagc gcctgccgat tgaacgtatt caactccgtc
    13381 cgcactgtag cattggcgtg gcgatgttct acggcgatct caccgccgaa cagctttaca
    13441 gtcgcgctat ttctgcggca tttaccgctc gccataaagg caagaatcag attcagttct
    13501 ttgatccgca gcagatggaa gcagcccaga agcggttgac ggaagagagc gatatcctta
    13561 atgcactgga aaatcatcag tttgcaattt ggttacagcc acaggtcgag atgaccagcg
    13621 gtaaactggt cagtgcggaa gtgttactgc gtatccagca accggatggc agttgggacc
    13681 tgccggatgg cttaatcgat cgcattgagt gctgtgggct gatggttacc gtcggtcact
    13741 gggtgctgga agagtcctgt cgattgcttg cagcctggca agagcgcggc attatgctgc
    13801 ccttgtcggt aaacctctct gcgctgcaac tgatgcaccc gaatatggtg gcggatatgc
    13861 tggaactgtt aacccgctat cgcattcagc cgggaacact gattctggaa gtgacagaaa
    13921 gccgacgtat tgacgaccct catgctgcgg tggcaatcct ccgtccgctg cgcaatgccg
    13981 gagttcgggt ggcgctggat gatttcggca tgggctacgc agggctgcgt cagctgcagc
    14041 atatgaaatc gttgccaatc gacgtactga aaatcgacaa aatgtttgtt gaaggcttgc
    14101 cggaagatag cagcatgatt gctgcaatta tcatgctggc gcagagcctg aacttacaaa
    14161 tgattgccga aggcgtggag actgaagcac aacgagactg gctggcaaaa gcgggcgttg
    14221 gtattgccca gggcttcctt tttgctcgcc cactccctat tgaaatcttc gaagagagtt
    14281 acctggaaga aaagtagcta ccccaaactg attacaaaac tttaaaaagt gctggtttgt
    14341 gcgagccagc tcaaactttt taaccttttt gtttcaatta tgatccaggt acatttctgt
    14401 gatgttgtct gggtgttatt ttaaggccgc
//
LOCUS       DAZOYF010000101        14319 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZOYF010000101 DAZOYF010000000
VERSION     DAZOYF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.101"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..3052
                     /locus_tag="ACHUOF_002797"
                     /pseudo
     CDS             1..3052
                     /locus_tag="ACHUOF_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3062..6708
                     /locus_tag="ACHUOF_002798"
                     /pseudo
     CDS             3062..6708
                     /locus_tag="ACHUOF_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6769..7176
                     /locus_tag="ACHUOF_002799"
     CDS             6769..7176
                     /locus_tag="ACHUOF_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611910.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7718..8806
                     /locus_tag="ACHUOF_002800"
     CDS             7718..8806
                     /locus_tag="ACHUOF_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFR0611911.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8817..11096
                     /locus_tag="ACHUOF_002801"
     CDS             8817..11096
                     /locus_tag="ACHUOF_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFR0611912.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11089..12225
                     /locus_tag="ACHUOF_002802"
     CDS             11089..12225
                     /locus_tag="ACHUOF_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR0611913.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12222..14225
                     /locus_tag="ACHUOF_002803"
     CDS             12222..14225
                     /locus_tag="ACHUOF_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611914.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3547 a         3755 c         3654 g         3363 t
ORIGIN      
        1 tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc tgactacttg
       61 gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc ggaagagtct
      121 ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc agaatggcgg
      181 caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga aattgcccag
      241 catctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt ggcgactgat
      301 gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt tgatgattac
      361 tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc agccctgccc
      421 cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca tatcaatcat
      481 ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg tcacgatcga
      541 atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagcactggc ggaacttcta
      601 gtgcaaaaag aagagaatag ttggcgcatt atgctaatga cgatgtttat ctcgcaacca
      661 gcactggcag aacaggtcat tccctggctt tcgacacccg cagttgcagt gctgaaatca
      721 tgccagcacc aactgacaca gccctcaaac catgccagcg ccgatctact gccagccata
      781 gtagtctccc caccatggat ttcgaaaaag aaaaaatcgc cgattccggt gctggattta
      841 gcgccattag gcatcgagcc aatctgttat ctgacagaag aaatcagtaa tcaacttttg
      901 gcgaaatata tctggtattc aaaacacatc acggttagcc atgaagaaag tactaccaac
      961 ctgttggcaa ggatgggttt tcaacgacgg atcgctggta catatattaa agctcccgaa
     1021 gcggtagttg aggcatggct aaatgaagat tattcaacct tactaagtga atttaaggtg
     1081 tttcattcac ctaccgggca ttattggcag ttggggattt tgacaacatt gccgctggag
     1141 gctggagaaa gcagtaaaag catggaatgc ccttactcta tctccacata ccgataccga
     1201 atacgccatg ttacattttg gactcaaagg tttacctggt ttagttaact caattacacg
     1261 ctatccacag gaagccttgc ccatcacgaa ttacttcgca gcgagtgagc tggctcctgc
     1321 cgtcgcccgt gccttcaaca aactcaaaac gctacgcgaa aacgcccgca cctggctgtt
     1381 gaaatacccg gaacatgccc ttaccggcct gctgcctgcg gcgctcggca aagccggtga
     1441 agcacaggat aacgcccgcg ctgccttgcg tatgcttacc gaaaacggtc atcagccatt
     1501 actgcaagaa atcgcccggc gttataacca gtcggaagta acagacgcgg tgaacgctct
     1561 gcttgcgctc gatcccttag ataatcaccc gacaaaaatc cccactcttc cgacctttta
     1621 tcagccatcg atctggacgc gcccggtatt aaaagcaaac gcccaatcac tgccagataa
     1681 cgccctcctc cacctcggcg aaatgctccg cttccctcag gaagaggctc tgtatccggg
     1741 attattgcag gtgaaagacg cctgtaccgc tgactctctg gctgaatttg cctgggatct
     1801 gtttaccgcc tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt
     1861 aggcgttctc ggtaacgatg acaccgcccg caaactgacg ccattaatac gcgcctggcc
     1921 tggtgaatcc cagcataaac gcgccaccgt tgggttggat attctcgccg ctatcggtag
     1981 tgatatcgcc ctgatgcagc ttaacggcat tgctcagaaa ctgaaattca aagcattaca
     2041 ggagcgggca aaagaaaaga ttgccgacat tgccgagagc cgcgaactca cggtagcgga
     2101 gcttgaagat cggttagcac cggatctcgg tctggatgat aacggttcgt tgctgctgga
     2161 ttttggccca cgccagttca ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga
     2221 tgtttccggc agccgcctga aagacctgcc aaaaccaaac aaaagcgatt atgagtcaca
     2281 ggcgaacgat gctgttaacc gctacaaatt gctgaaaaaa gatgcgcgta ccgtcgcggc
     2341 ccagcaggta gcaaggcttg aatccgccat gtgcctgcgt cgccgctggt cgccggaaaa
     2401 cttccaactc ttcctggttg tgcatccgct ggttcgccac ttaacccgcc gtctgatttg
     2461 gggcgtttat agcgccgaca accagctact ggcctgtttc cgggtagcgg aagataacag
     2521 ctacagcacc gctgacgatg atcttttcac cctgccggaa ggcgatatct ctatcggcat
     2581 tcctcacgtt ctggaaatat caccgacgga tgctgtcgcc tttggtcagc tttttgccga
     2641 ctacgaactg ctaccaccgt tccgtcagct tgaccgtaac agttacgccc tgacagaagc
     2701 cgagcgcaac gccagcgaac tgacccgctg ggcaggcaga aaatgcccga gcggccgggt
     2761 catggggctg gcaaataaag gctggataaa gggcgaacca caggatggag gctggatgat
     2821 caaacctctc ggtcgctggt cgttaatcat ggaaatcgac gaaggttttg cggtgggtat
     2881 atcaccagcc gaacttagcg ctgagcagct cttaagcaag ctgtggctat gggaaggcaa
     2941 agcggaaagc tatggctggg ggagtaattc aacacaggaa gcgcagttct ccgtactcga
     3001 tgccatcacc gccagcgagc taattaacga tattgaagcc ctgtttgaat aaggaaagag
     3061 catggacaag gaattaccgt ggctggcgga taacgcccaa ctggaactga aatataaaaa
     3121 aggcaaaaca ccactcagtc atcgcaattg gccgggcgaa ccagtgcctg ttatcactga
     3181 aagcatcatt cagacactgg gtgatgaatt gctacaaaaa gctgagaaga aaaaaaacat
     3241 tgtctggcgt tatgaaaatt tttcaccgga gtggcagtcc gccattacgc aggccatcaa
     3301 cttgatcggc gaacacaaac cctcagtccc ggcccgcaca atggcggcac tagcctttat
     3361 cgcgcaaaat gacaaccaac agttgctcga cgaaatcgtc caacaagagg ggctggaata
     3421 tgcgactgag gtggtgattg cacgccagtt tattgcacgg tgttatgaga gtgatcctct
     3481 ggtagtcaca ttgcagtatc agaacgagga ctatggctat ggttatcgct cagaaaccta
     3541 taacgaattc gatctccgac tgcgtaaaca cctctctctg gcagaggaaa gctgctggca
     3601 gcgttgcgcc gacaaactca ttgccgcact gccaggaata accaaagttc gccgcccttt
     3661 tattgcgctg atcctccccg aaaaaccaga aatcgccaat gagttggtaa gccttgaatg
     3721 cccgcggact cattttcatt ctaaggagtg gttaaaagtt gttgctactg accccaaagc
     3781 ggtgagaaaa ctcgaacgct actggagcca ggatatattt agcgatcgag aagccagcta
     3841 catgtcgcat gaaaaccact tcggctacgc ggcctgcgct gcccttttgc gcgaacaagg
     3901 actggcagtc gttccgcgcc tcgcgatata tgcccataaa gaagattgcg gcagtctgct
     3961 ggtacaaatt aaccatccgc aagtcatccg caccttgctg ttagttgctg ataaaaacaa
     4021 acccagcctg caacgtgtgg ctaaatacag taaaaacttc ccccatgcga cactcgccgc
     4081 actggcagaa ctgctggcgt taaaagaacc accagcccgc ccaggttatc caatcatcga
     4141 agacaaaaag ctgcctgcac agcaaaaagc tcgagatgaa tactggcgta cgctgttaca
     4201 aacgctgatg gcatcgcagc cacaactggc agcagaagtg atgccgtggt taagtactca
     4261 agcccgggca gtgctgaata gttatttatc ggcaccgccc aaaccagtta ttgatagcac
     4321 cgataacagc caaatgcctg aaatcctcgt ttcaccacca tggcgtggga gaaagaaaaa
     4381 gacttccata cgtcactttt cgctgcctga tcttacgctt gcaccgcaaa cgtacaggtc
     4441 gccgaataaa cactggacgc cggaacagca aactgcaatt gaccatttta gcgccatgtc
     4501 ttttgacgaa cgtcttgcta cgagaggagc ggatatcttc ttgcgggaat taggttttga
     4561 cgttcatagc ctggaattta ataagcgtct tcttaatgga cagttggatg cctttgatgc
     4621 tcagcaactg caagaataca catcaatagg actattgcgt acttatcacg cgcatcaggc
     4681 caaatctaaa cagtaccggg aagacgccac caatgcatta ctggcacaag atagcattgc
     4741 gttgatggga gggtggcgaa cttttcagca cggattttac tatggtaatg aacagcgttg
     4801 ggggatttgg aatctgtacc ttatcgcgca gatgccgcgc gaaatggcgg tatcgtgctg
     4861 gcaacaaatt gtcgaggctg attttcatta caccggggtg gaatatttgt taagtgtgct
     4921 gggtaccgat gctttgcctg gattaaacac cgcctttgcc cgacatccaa aagagatatt
     4981 tccgctgctg attcatttcg gcgcaacaga actggccctg cccattgccc gcgtctggca
     5041 cctttttgcg ggccagcgta atctggctcg ccagtggatt ttacaatggc cggaacatac
     5101 ggctactgca cttatccctc ttgtcttcac caaatctagc gataaaagcg aagctgcatt
     5161 acttgccctg cgtttacttt acgaacatgg gcatggcgaa tggctacaaa ctgtagccaa
     5221 tcgttggcag cgtacagatc tatggcctgc cctggaacaa ttacttaaac agagtccaat
     5281 agagatttac ccggcacgca ttccaaaagc ccctgatttc tggcatccgc aaatgtggtc
     5341 caggccgcgc cttatcacta ataatcaacc tgttaccgat gacgctctgg aaattatcgg
     5401 cgaaatgctg cgctttaccc aggggggacg tttttatagc gggctggaac aactgaaaac
     5461 gttctgccag ccacaaacgc tggcagcttt tgcctgggat ctcttcactg cgtggcaaca
     5521 agctggtgcc cccgcaaaag acaactgggc atttctggcg ttaagtctct ttggtgacga
     5581 aagcacggca cgggatctaa cgacacatat cctcgcctgg ccacaagaag gcaaatctgc
     5641 ccgtgcggtc agtggcatga acatccttac cctgatgaat aatgatatgg cgctgatgca
     5701 gctgcatcat atatcgcaac gggcgaaatc ccgcccctta cgtgataacg cggcggaatt
     5761 tcttcaggta gtcgcagaaa atcgcgggct aagccaggaa gagttagcgg acagattagt
     5821 cccaacactg ggccttaatg atccgcaggc gttgagtttt gattttggtc cccggcagtt
     5881 taccgttcgc ttcgatgaaa acctcaaccc ggttatcttt gatcagcaaa acgttcgcca
     5941 gaaaagtgtt ccccggttgc gcgccgatga cgatcaactg aaagcgcccg aggcactggc
     6001 ccgactaaaa gggttaaaaa aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct
     6061 tgaagctgcc ctacgtacca cccgacgctg gtcgctggca gattttcatt ctctgtttgt
     6121 taatcatccc tttacccgtc tggttaccca gcgattaata tggggggtta tccggcaaat
     6181 gaaccgcgtc gtttactcaa cgcctttcgt gtggccgcag agggggagtt ctgcaatgcg
     6241 caagatgagc caattgacct gcctgcggac gctctgattg gcattgccca cccgttagaa
     6301 atgacagcag aaatgcgcag tgaatttgca cagctttttg ccgattacga aattatgccg
     6361 ccttttcgcc agttgtcgcg ccgcacggtg ctgctcacac ctgacgagtc aaccagtaac
     6421 agcctgactc gctgggaagg taaatccgct accgttgggc aacttatggg aatgcgatac
     6481 aaaggctggg agtcaggtta tgaggacgca tttgtctatg acctgggtga gtaccggctg
     6541 gtccttaagt tttcacccgg ttttaaccac tacaatgttg atagcaaagc gctaatgagc
     6601 ttccgttctc ttcgagtgta ccgtgacaat aaatccgtca cttttgccga acttgatgtg
     6661 tttgatttga gtgaggcgtt aagcgcacct gacgtcattt tccattaaaa caaagcgggc
     6721 agtgatgcga ctgcccgtta tctacacgac ttaccagcgg ggaaagcgat gatcgtgcaa
     6781 aaagagctgg ttgctattta cgattatgag gtccctgtac ctgaagatcc gttttccttc
     6841 agacttgaga tccataaatg ctctgaatta tttacaggtt ccgtctaccg actggaacgg
     6901 ttccggctac gtccaacatt tcatcaacgt gatcgagagg atgttgaccc gctaataaat
     6961 gatgcgttga tttatataag agatgagttt attgatgagc aaaaattacg aggcgaatca
     7021 cctgaacctg taatagtaat ttttaattgt aaactacaga atatattcaa ccaacaaata
     7081 gaattatata ctctagataa ttcaagtcgc agcacatgca acttgaagta tgacgagtat
     7141 agctttatta aaggggggaa cggtattccc ccttagatgc gtttcaattt gcccgttact
     7201 tcaacaaatc aatcaaccgt aacagagcca ctaatagctc cagtacagca atgagcacgg
     7261 ttaacttttg tgtcagcgtg tccatccgct ctccttatta aggagcacaa caccagcacc
     7321 ttacccttac actgcctgtc gatactggtt tacgtttgtt gagtcgccat gttgcactcc
     7381 ggatcagagc actacctgcg gcaccacccg tccggtagcc aggacttgaa ataggttcga
     7441 tccggcgcaa cttcccaaca gtcaggatta taaatctcat tatcttaatt gcaacactgt
     7501 ccagaggtgg ttataattcg ttcgttggaa cacgatgttg cacaggcagt ggtgttaggc
     7561 ctgaaaatag taaataaaag aacctgcctc accagcagtt ttttttattt actgtgatct
     7621 gctttccaga tatttttcgc tcaaacaact aatgcgccaa acatttattg cgcgtaaaat
     7681 atcgtttatt tcattaatac atttcaggga tgaatatatg tcaccgcaga ataatcatct
     7741 gcagcgtccg cctgctgctg tgttatacgc cgatgaactg gcaaaattaa aacaaaatga
     7801 taacgcacct tgcccgcccg gttggcagtt aagtttgcct gcggcccgtg cttttatcct
     7861 tggcgatagc gcgcaaaata ttagccgtaa agtggtgatt agtccctccg ctgtcgaacg
     7921 tatgttagtc actcttgcca ccggacgcgg tttgatgttg gtgggggaac ctggcaccgc
     7981 aaaatctctc ctgtctgaat tactggcaac ctctattagc ggcgatgccg ggttaaccat
     8041 ccagggcggc gcatctacta ccgaagatca aatcaagtac ggctggaact acgccctgct
     8101 tatcaatcat ggtccatcaa cggaagcgct ggtccctgcc ccgctttacc agggaatgcg
     8161 cgacggcaaa atcgtccgtt ttgaagagat aacgcgtaca ccgctggaag tgcaagactg
     8221 tctgttaggt atgctttctg acagggtgat gacggtgccg gaactcactg gtgaagccag
     8281 tcagctctat gcgcgagaag gctttaatat tattgccact gccaataccc gcgatcgcgg
     8341 cgtcaatgaa atgagtgctg cgctaaagcg tcgcttcgat tttgaaactg tctttccgat
     8401 tatggatttc gcccaggagc tggagcttgt cgccagtgct tcggcgcgtt tgctggccca
     8461 tagcggtatt ccgcataaag tgcctgacgc ggtactggag ttactggtcc gcaccttccg
     8521 cgatctgcgt gccaacggcg aaaagaaaac gtcaatggat acactaacag cgattatgtc
     8581 caccgctgaa gccgtgaacg tggcccacgc cgtaggcgtc cgcgcctggt ttttagcgaa
     8641 tcgcgcaggt gagccagccg acctggtgga ctgcattgcc ggaaccatcg tcaaagataa
     8701 cgaagaagat cgcgctcgtc tgcgccgtta cttcgaacag cgtgtcgcca cacataaaga
     8761 agctcactgg caggcttatt atcaagcccg ccaccgcctg ccgtgaggaa agatgcatga
     8821 gcgagccgtt aattgtcggc atccggcatc atagtccggc ctgcgcccgg ctggtgaaat
     8881 cgttaatcga aagccagcgg ccacgatacg tgttgattga aggccctgct gattttaatg
     8941 accgggtaga cgaactgttt ttagcccacc agcttccggt agctatttac agttattgcc
     9001 agtatcagga cggtgcagcc cccgggcgtg gtgcctggac gccatttgct gaattttcgc
     9061 cggagtggca ggcgctacaa gccgatcgtc gtattcaggc acaaacttac ttcatcgatt
     9121 tgccttgctg ggcgcagagt gaagaagagg acgattcgcc tgatacgcaa gaggaaagcc
     9181 agaccttgct gctgcgtgcc acccgcatgg ataacagcga caccctgtgg gatcacttgt
     9241 tcgaagatga aagccagcaa actgcattac cctctgcact ggcgcactat tttgcccaac
     9301 tgcggggcga ctcccccggc gatgctctaa atcgtcagcg cgaagccttt atggcccgct
     9361 ggattggatg ggcgatgcag caaaataatg gcgacgtgtt agttgtctgc ggtggctggc
     9421 acgctccggc actggcaaaa atgtggcgcg aatgtccgca ggtaattaac acgccagaat
     9481 tgccgtcgct ggcagatgcc gttacaggtt gttatctcac cccctacagt gaaaagcgcc
     9541 ttgatgtgct ggcaggatac ctttcaggaa tgcctgcccc ggtctggcaa aactggtgct
     9601 ggcagtgggg cttacagcag gcgggagaac aactgctaaa aacggttctc acccgtttgc
     9661 gccagcacaa gttgcctgct tcgaccgcgg atatggctgc cgctcatctg catgcgatgg
     9721 cactggcaca gttgcgcggt catacactac cgttacgcac tgactggctg gatgccatag
     9781 caggatcgct gattaaagaa gccctgaatg cgccgttgcc gtggagctat cgcggcgtta
     9841 ttcatcccga taccgatccg attctgctaa cgttgataga cacattagcg ggtgacggat
     9901 tcggtaaact tgccccttct acgccacaac cgcctctgcc aaaagatgtc acttgcgaac
     9961 tggaacgtac cgcaatctct cttccggcgg agcttacctt aaatcgcttt aaccccaatg
    10021 gactagcgca aagtcaggtg ttacatcggc tggcaatact ggagatccca gggattgtac
    10081 gccagcaggg aagcacactg acacttgcag gcaacggtga agaacgctgg aaattaaccc
    10141 gcccgcttag ccagcatgcg gcattgattg aggccgcctg ctttggtgcc acactcctgg
    10201 aagccgcacg ccataaatta gaagccgata tgctggacgc gggtggaatc ggcagcatta
    10261 ccacatgtct tagccaagcg gcgtcagcgg gtctggcgtc cttcagtcaa caattactgg
    10321 agcaactcac attattaatc gcccaggaaa atcaatttgc cgaaatgggc caggcgctgg
    10381 aagtgctata tgccttatgg cggctggatg aaattagcgg tatgcaaggc gcgcagatat
    10441 tacaaacgac gttatgcgcg gctatcgatc gcacgctgtg gctgtgtgaa tccaacggca
    10501 gaccggatga aaaggagttt cacgctcact tgcatagctg gcaagcgttg tgtcatatcc
    10561 tgcgcgatct acatagcggt gtgcaactcc ccggcgtttc gctttctgcg gcggtagcct
    10621 tgctggagcg acacagtcag gcgattcatg tcctggcact ggatcgcggc gcgactcttg
    10681 gtgcgctgat gcgtctggaa catcccaacg ctagtgccga agcggcgctg acgatgctgg
    10741 cgcagttatc cccggcacaa tccggcgagg cgctgcacgg tttgctggca ttagcccgtc
    10801 atcaactggc ctgtcagccg gcatttatcg ccggtttcag cagtcattta aatcaactaa
    10861 gtgatgccga ttttaccaat gccctgcccg atttacgcgc ggcaatggcc tggctaccgc
    10921 cacgagaacg cgggacgctg gcgcatcagg tgcttgagca ttatcaactg gcgcaacttc
    10981 ccgtttcggc actgcaaatg ccgttgcatt gtccaccgca agccattgca catcatcaac
    11041 aactcgaaca gcaggcactg gcatcgctgc aacactgggg agttttccat gtctgaactg
    11101 aacgatcttc tgaccacccg tgagctacaa cgctggcgat taattcttgg cgaagcggca
    11161 gaaacgacgc tttgtgggct ggatgacaac gcccggcaga tagatcacgc gctggagtgg
    11221 ctgtatgggc gcgatcctga acggctccag cgtggtgaac gctctagtgg attaggtggc
    11281 tcaaatctca ccactcctga gtggatcaac agtattcaca cgctgtttcc gcaacaggtg
    11341 attgagcggc tggaaagcga tgccgtactg cgctacggca ttgaagatgt ggtgacaaat
    11401 ctcgacgtgc tggaacgtat gcagccttcc gaaagcctgc tacgcgccgt tttgcacacc
    11461 aaacatctga tgaaccccga agtactggct gccgcccgca ggattgtgcg ccaggttgtg
    11521 gaagaaatta tggcgcgact ggcaaaggaa gttcgtcagg ctttttctgg tgtccgcgat
    11581 cgtcgtcgcc gctcatttat tccactggcg cgaaactttg atttcaaaag tactctgcgc
    11641 gccaacctgc aacactggca cccgcaacac ggcaagttgt atatcgaatt cccccgcttt
    11701 aacagccgca ttaaacgcca aagcgagcaa tggcaactgg tcttactggt tgatcaaagc
    11761 ggatcgatgg tcgattcggt gatccactct gctgtgatgg cggcctgttt atggcagtta
    11821 cccggcattc gtacccatct ggtggcgttt gacacaagcg tcgttgatct cacggcagac
    11881 gttgccgatc cggtagagtt attaatgaaa gtgcaattag gcggcggaac caatatcgcc
    11941 agtgccatgg agtatggtcg gcaacttatt gaacaacctg caaaaagcgt cattatcctc
    12001 gtgagcgatt tttatgaagg gggttcatca tcattgctga cgcaccaagt gaaaaagtgt
    12061 gtccagagcg gcatcaaagt gctgggactg gcagcgctcg atagcacagc aacaccttgc
    12121 tatgaccgcg atacggccca ggcgctggtt aatgtcggcg cacaaatagc cgccatgaca
    12181 ccgggcgagc tggcatcatg gcttgcggag aatcttcagt catgaattca ctacgtccgg
    12241 aattattaga actgacaccg caggcactga cggcgttaag caatgccggt tttgttaagc
    12301 gcagccttaa ggaactggaa aatggcaacg tcccggaaat cagccatgag aacagcgctt
    12361 taatcgccac cttcagtgac ggtgtccgta cccagctggc gaacggccag gcactgaaag
    12421 aggctcagtg cacctatggg gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa
    12481 gttatcaacg actttgtgct accgcccagc ctacgggaaa agaagaggaa tgggatccgg
    12541 caatctggct ggaagaactg gctacccttc ccgatgccac ccgcaaacgc gcacaggcgc
    12601 tggtcgctaa aagcatcacc attgagttgt tctgtgcgcc gggtgaaatt ccctctgccc
    12661 gcttaccgat gagcgatgtg cgtttttatt cccgcagcag tattcgtttc gcccgttgtg
    12721 attgtattga aggcacactt tgcgaacatg tcgtactggc agtacaggcc tttgtccagg
    12781 caaaagcgca gcaagcggaa ttgactcatt taatctggca gatgcgcagc gaacacgtca
    12841 catcatctga cgatccgttt gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcaac
    12901 aactaagcca ggcattatgg ctgagcggca tcagccagcc gcttattcac tacgaggccg
    12961 ctttcagccg cgcgcagcaa gcagcggaac gctgcagctg gcgatgggtg agtgaatcgc
    13021 tacggcaact gcgcgccagc gttgatgcct tccacgcccg cgccagccac tatcatgccg
    13081 gagaatgctt acgtcagctt gcggcattaa acagtcgatt aaattgcgca caagagatgg
    13141 cccggagcga cagtgttggt gaagttcctc ctgtgccgtg gcgcacggtc gttggctctg
    13201 gcattgccgg agaagcaaag cttgatcatc tgcggctggt gtctttaggt atgcgttgct
    13261 ggcaggatat tgagcattat ggtttacgca tctggtttac cgatcccgac accggcagta
    13321 ttttgcacct ttcgcgcagt tggccgcgaa gtgaacagga agactcaccg gcagctacgc
    13381 gtcggctgtt tagttttcag gctggcgcac tggcgggtgg gcaaattgtt tcacaagcag
    13441 caaaacgcag tgccgatggc gagctgctgt tagctacccg caaccgctta agcagcgttg
    13501 taccgctgtc gcctgatgcc tggcaaatgt tgagcgcgcc gttacgccag ccgggcattg
    13561 tggctttgcg ggaatattta cgccagcgtc cccccgcctg catacggcct cttaatcagg
    13621 tcgataactt atttattctg ccggtcgctg agtgtatttc gctcggttgg gatagcagcc
    13681 gccagacgct ggatgcgcag gtaatcagcg gcgaaggtga agataatctg ctgacgttat
    13741 cactgccagc ctcagccagc gcgccttatg ccattgaacg catggcggcg cttttgcaac
    13801 aaacagacga ccccgtgtgt ctggtttctg gctttgtcag ttttgttgat gggcaattga
    13861 cactggaacc acaggtaatg atgacaaaga ctcgtgcctg ggcgctggac gcagaaactg
    13921 cgcctgtggt ggtatcacta ccttctgcca gcgttctgcc tgtgccgtct actgctcatc
    13981 agttgctgat gcgctgccag gcgttactta ttcaactgct ccataacggc tggcgctatc
    14041 aggaacagag cgctattaat caggcagagt tgctggcgaa tgacctcacc gcggtgggtt
    14101 tttatcggct ggcacatgtg ttggcacaat ttcgtaatac agaaagcgag gcacgggtag
    14161 aagcaatgaa taacggtgtt ttgctttgcg aacaattatt ccccatgctt cagcaacaag
    14221 gatgaaatag tgctttttac taagagttct actccagttc cggactgctc acgccacggt
    14281 attaggcata tcctatatag cccctggtga gagtcacca
//
LOCUS       DAZOYF010000102        14102 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZOYF010000102 DAZOYF010000000
VERSION     DAZOYF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.102"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            6..407
                     /locus_tag="ACHUOF_002804"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHUOF_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..4690
                     /gene="pic"
                     /locus_tag="ACHUOF_002805"
     CDS             572..4690
                     /gene="pic"
                     /locus_tag="ACHUOF_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HFR0611915.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4841..5083)
                     /locus_tag="ACHUOF_002806"
                     /pseudo
     CDS             complement(4841..5083)
                     /locus_tag="ACHUOF_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5122..6072)
                     /locus_tag="ACHUOF_002807"
                     /pseudo
     CDS             complement(5122..6072)
                     /locus_tag="ACHUOF_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6123..6698)
                     /locus_tag="ACHUOF_002808"
                     /pseudo
     CDS             complement(6123..6698)
                     /locus_tag="ACHUOF_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6702..7102
                     /locus_tag="ACHUOF_002809"
                     /pseudo
     CDS             6702..7102
                     /locus_tag="ACHUOF_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7505..8086)
                     /locus_tag="ACHUOF_002810"
     CDS             complement(7505..8086)
                     /locus_tag="ACHUOF_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611916.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(8133..11990)
                     /gene="sigA"
                     /locus_tag="ACHUOF_002811"
     CDS             complement(8133..11990)
                     /gene="sigA"
                     /locus_tag="ACHUOF_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR0611917.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(12351..13355)
                     /locus_tag="ACHUOF_002812"
                     /pseudo
     CDS             complement(12351..13355)
                     /locus_tag="ACHUOF_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            13340..13717
                     /locus_tag="ACHUOF_002813"
                     /pseudo
     CDS             13340..13717
                     /locus_tag="ACHUOF_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13756..>14102)
                     /locus_tag="ACHUOF_002814"
     CDS             complement(13756..>14102)
                     /locus_tag="ACHUOF_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0611918.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         3540 a         3315 c         3457 g         3790 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgtccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgggta gtggtacgca gtacactaag gacaaggacg gaaatttagt
     1201 taaggttgcc ggtggatatg cttttaaaac aggaggaacc acaggagttc ctctgatatc
     1261 tgatgcaaca atagtctcta atcccgggca aacttataat cctgtaaacg gccctttacc
     1321 tgactatgga gcccctgggg atagtggttc tcctttgttt gcttatgata aacaacaaaa
     1381 aaaatgggtt attgttgctg tattaagagc atatgcaggt attaatggtg ctacgaactg
     1441 gtggaatgtc ataccaacag attatctgaa ccaggttatg caggacgatt tcgatgcccc
     1501 cgtagacttt gtttccggac tgggccccct gaactggaca tacgacaaaa catcaggcac
     1561 aggtaccctg agccagggca gtaaaaactg gaccatgcac gggcagaaag acaatgacct
     1621 caatgccggt aaaaatctgg tattcagcgg gcagaatggt gcaattatcc tgaaagacag
     1681 tgtgactcag ggtgccggtt atctcgaatt taaagacagt tacaccgtat ctgctgaatc
     1741 cggaaaaaca tggacgggtg ccggcattat tactgacaag gggacgaatg taacctggaa
     1801 ggtcaacggc gttgccggtg acaacttgca taagctgggg gaaggaaccc tgaccataaa
     1861 cggaacaggt gtaaacccgg gaggactgaa aacgggagac ggtatcgttg tacttaacca
     1921 gcaggcagac actgcaggta atatccaggc cttcagttca gtgaacctcg ccagcggacg
     1981 tccgaccgtg gtgctcgggg atgcccgtca ggtcaatccg gataacattt catggggata
     2041 ccggggaggt aagcttgacc ttaatggtaa tgccgttacc ttcacccgac tgcaggctgc
     2101 tgattacggg gcggtgatta caaataatgc acagcaaaaa tcccagcttt tactggatct
     2161 taaggctcag gatacaaatg tcagtgaacc gacgattgga aatatatccc cctttggtgg
     2221 taccggcact ccaggaaacc tgtacagcat gatactcaac agccagaccc gcttctatat
     2281 tctgaaatct gccagctatg gtaacactct gtgggggaac agcctgaatg atccggctca
     2341 gtgggagttt gttggcatga acaaaaacaa agcagttcag acagtaaaag ataggatcct
     2401 ggccgggcgg gcaaaacaac ccgttatctt tcatggtcag ctgaccggga atatggatgt
     2461 cgccattcca caggtgccgg ggggaagaaa ggtaatcttt gatggtagcg tgaacctgcc
     2521 ggaaggtacc ctgagtcagg acagtggcac cctgatattc cagggacatc cggttatcca
     2581 tgcctccatc agtggcagtg caccggtcag cctgaaccag aaagactggg aaaaccgtca
     2641 gtttacaatg aaaacactgt cgctgaaaga cgctgacttc catctttcac gtaacgcctc
     2701 gctgaacagt gacattaagt cggataacag ccatatcaca ctgggaagtg acagggcatt
     2761 tgtggataaa aatgacggaa caggaaatta tgtcattccg gaggaaggta cctctgtccc
     2821 ggacaccgtg aatgacagga gccagtatga agggaatatt acgctgaacc ataactcagc
     2881 cctggatatc ggcagcaggt tcaccggggg gattgacgct tatgacagtg ccgtcagcat
     2941 cacctctccg gacgtcctgt tgacagcccc gggtgctttt gccggcagtt cactgacagt
     3001 gcatgatggc ggtcatctta cagcactgaa cggtcttttc agcgacgggc atattcaggc
     3061 cggtaagaac ggcaaaatca ccctgagcgg tacaccggtt aaagatacgg ctaatcagta
     3121 tgcccctgct gtatatctga cggacggata tgacctgacc ggcgataacg cagcactgga
     3181 aattacccgt ggagcacatg cttccggtga tattcatgcc tctgcggcat caacagttac
     3241 catcgggtct gacacgccgg cagaactggc ttctgcggaa acggctgcat cggcgtttgc
     3301 cggcagtctt cttgagggct ataacgcagc attcaatggt gccataaccg gtggcagggc
     3361 tgatgtcagt atgcataatg cactgtggac tctgggtggg gactctgcca tccacagtct
     3421 taccgtcaga aacagccgta ttagttctga aggagaccgt acattccgta ccctgacggt
     3481 gaataaactg gatgcaacag gcagtgattt tgttttgcgt acggacctga aaaatgccga
     3541 taaaattaat gtgactgaaa aagccactgg ttcagataac agcctgaacg tcagctttat
     3601 gaataatcct gctcagggac aggccctgaa tattcctctg gtcacggcac cggcgggaac
     3661 ttcagcagag atgtttaagg ccggcacccg ggtgacaggt ttcagtcggg tgaccccaac
     3721 cctgcatgtt gataccagtg gtggcaatac gaagtggata ctggatggtt ttaaagcgga
     3781 ggctgataaa gccgctgccg cgaaggctga cagtttcatg aatgccgggt ataaaaactt
     3841 catgacggaa gttaacaatc tgaacaaacg tatgggtgac ctgcgtgaca caaacggtga
     3901 tgccggtgcc tgggcgcgca tcatgagtgg tgccggttct gcagacggtg gttacagtga
     3961 taattacacc catgttcagg tcggctttga caaaaaacat gaactggacg gtgtggacct
     4021 gtttaccggt gtcacgatga cctataccga cagcagtgca gacagccatg cattcagcgg
     4081 aaagacgaaa tcggtggggg gcggtctgta tgcttcagca ttgtttgagt ccggtgccta
     4141 tatcgatttg attggtaaat atattcacca tgacaatgat tacacaggta actttgctag
     4201 cctgggaacg aaacactaca acacccattc ctggtatgcc ggtgctgaaa cgggttaccg
     4261 ctatcacctg acagaggaca cgttcattga gccgcaggct gaactggttt acggcgccgt
     4321 gtccgggaaa acattccgct ggaaagacgg tgatatggac ctgagcatga agaacaggga
     4381 cttcagtccg ctggttggaa gaacaggggt tgaactgggc aagaccttca gtggtaagga
     4441 ctggagtgtg acggcccgtg ccggaaccag ctggcagttt gacctgctga ataatggaga
     4501 gaccgtactg cgtgatgcgt ccggggagaa acggataaaa ggagagaagg acagccggat
     4561 gctgtttaat gttggtatga atgcgcagat aaaggacaat atgcgctttg gtctggagtt
     4621 tgagaagtca gcctttggta aatataacgt ggataatgcg gtaaacgcga atttccggta
     4681 tatgttctga tgtgggggta aagggctgcc ggaaggcagc ctttctcaga gactcttaaa
     4741 atataaaact gaaggtcttc ggtaaggggg agtgtattag acgtcaacta gttttgccga
     4801 ctggcgcgag cctgtttaag attctgtgta aatgcctttc tcagaagtga ccgtccaggc
     4861 ggtcaccgaa ctcgataata aagcggctca ttgccattcg ccagtccttc aacggcatcg
     4921 tccatttctg ggacgcagac tggattgcca gccacaccac ttttttcacc gagtcgtctg
     4981 tcgggaacac tttacgcttt ttgatcgcat ggcggatcac gctgtttagc gactcgatgg
     5041 cattcgtcgt atagatcact ttgcggatgt ccgttggata agcggtaagc gtacagcgag
     5101 ggccgtattg acggggatgt gttattcagc tggcagtgct atgcgccacg gaagcagttc
     5161 gctgacccgg ttgaccggcc agtctgctat gacgccaagc acatggcgaa ggtagctttc
     5221 tggatccacg tcattcagtt tgcacgtccc gatcaggctg tacagtagcg cgccccgctc
     5281 accaccatgg tcagagccga agaacaggaa gtttttacga cccagactga ccgcccgcag
     5341 ggcattttca gcgatgttgt tgtcgatttc cacccagcca ttactgcagt acacgttcag
     5401 ggccttccac tgtttcagta ggtatgcgaa cgcttttgcc gtatccgagt gacgcgacag
     5461 tgttttcatc tgttgctgta tccggtcata cagtgactgc atcagtggcg cggctcttgc
     5521 ttttcttgcc gccagacgct gttctgctga acatccccgg acttctgcct cgatggtata
     5581 cagttcaccg atacgctgca gggcttccgt ggtgatgtcg gttggcactc ttgcatgcac
     5641 atcgtggatt tttctccggg catgggccat acacgcagct tccgttattc tgccggattc
     5701 gtataacgcc cggtaaccac cgtaagcatc tgcctgaagc acaccgctgt aaccggctag
     5761 gtgattctgt ggatggatac ctttccggtc cgggctgtac gcgaaccaga ccgccggggg
     5821 catctgtgaa ccggcgttac ggtcatcacg gacatagacc cacagccggg cagttcttgt
     5881 tttaccactg cccggctcct ggaccggaac ggggatatca tccgcatgaa ctttaccggg
     5941 catcagtaca tactggcgca ggacgtcata cagcggctcc agcagttcag caacggcacc
     6001 ggtccagcga cccagtgtgg cacggctcag ctccacgccc tgacgacggt atatttctga
     6061 ctggcggtat aactgtcaac gacggatgaa aagtggctct tccgcaacct gggtggagat
     6121 tttcatgcca gtggactcat taccctctgc ctgagtagtt caaatcctgc tcgaccatac
     6181 atctggcgtt tcagcatctt cttcaggcga tttacatgac cttcgacaac accattactc
     6241 cagcgactac tgattgcctc acatatagct gcagcatcag cttccatccc cgccgccacg
     6301 cgccgaaatt ctgctgaacc gctttcgtgg actcgagtga accaactgct tagctgtgat
     6361 ttattttggg ttttcagaat acggtagaac tcgagtgcca gttgctgcgc tattttcagc
     6421 tcaggttctt tttcacacat cagactaata aatcgggaag cataattttc ttcatccctg
     6481 gttattctcc agggcatcaa ccagcggctt acccgggaga ctgaaggaag tctgacggca
     6541 gtagtaaccg gtgggttcca gcctttacgc catttggcaa ccgcatccct gacgatggtt
     6601 tcactgcctg taaatccccg ggccaccatt tcccgccata tccggctggc gttataatta
     6661 ccactttctc gttgctcttt taaccactcc ctccatggtg tcagcgaaaa acgaaaccgc
     6721 tgttgaaatc cctggaaagc tggttgcgga aaagatgaag acccagtcgc gacactcaga
     6781 gttggcgaag gcgttcgcgt acgcacttaa ccagtggccg gcactgacgt actatgcgaa
     6841 cgatggctgg gtggaaatcg acaacaacat cgctgaaaat gccttgcggg cggtcagtct
     6901 gggtcgtaaa aacttcctgt tcttcggctc tgaccatggt ggtgagcggg gagcgctact
     6961 gtacagcctg atcgggacgt gcaaactgaa tgacgtggat ccagaaagct accttcgcca
     7021 tgtgcttggc gtcatagcag actggccggt caaccgggtc agcgaactgc ttccgtggcg
     7081 catagcactg ccagctgaat aacacatccc cgtcaatacg gccctcgctg tacgcttact
     7141 catcacctgc tattaatccc cgagtcagta cttcgcggcc tgacgcgatc accctactca
     7201 gtacttcaga gcccgcctcc ggtacctgcc tggcgatcgc ccctgtgcaa caaaataaat
     7261 caaccgttat caaaccacga tcgttcgtat gcgccgccga tgacgcgcgc ctgaaaaacg
     7321 cgtcaatatg agttccccgg aagcttacga taatacgctt atgaacatta caacacgctc
     7381 atgcggaacc tggatggggg aaggaacggt gaagctgtta gcgaatgacc ggcgtttata
     7441 caaacgccgg tcatgatgaa tgatatttca gaatatacct cgggctaaaa taccgggaca
     7501 gagatcaggc gtgattaatc tgcagcattt ttaataacag aagtactgag cttactttac
     7561 ctttgatgat gtaatagtta tttccgtcgg tactcatttt tccctgaaat aatgtcacgg
     7621 atgtttcttc ctgctgatcc tgcaggcgca gtcgtaaggt catattttta atgagctgag
     7681 atccgggtgg caaggggtat ttgctgtcag atactgggcg actgagtctg atgtggcgcg
     7741 tatacagccc gttttcgtac agggtggcct ctgttatggt gtcagtgacc agcatgtggg
     7801 agacaatgct gtattctttt ttaaagcacc caccagaaac ggtgattgta atgataatgg
     7861 tcttctcgat cgagatgtaa atacccgttt cgttatactg tccgtatcgg aaccatgaga
     7921 actcagttgc attccagcca tgctccagaa tatgtgcatt tttttgcctg atagtttctt
     7981 tttttattcg ctcatgttta aggcataaca ggcagataaa taatgtcaga aaaagaaagc
     8041 taataatgag agcaagagcg atatagtctg tcacagggta atacatattc tctccggtag
     8101 aagaagggcc gcaaacgcgg cccgggctgt tatcagaaag agtaacggaa gttggcgttg
     8161 atcgcgttat ccacgttgta tttaccaaat gccgatttct caaactcaag accgaagcga
     8221 agattatcgc gaatttcggc gttgagacca acattcatga gaatacgacc gtctttttca
     8281 cctttgatac gtttctcacc ggacgcatca cgcagtacgg tttcaccgtt ggcaaacagg
     8341 tcaaactggt agccaaggcc ggcgcgggct gtgactttcc agtccttacc ggagaaggat
     8401 ttacccacat caacaccggt acgcccaatc agcggattaa agtccttatc cttcatggtc
     8461 acgctcattc cgcgatcctg ccagtcgaac cgtttacctg atacggcccc ataaaccagt
     8521 tctgcctgcg gctcaatcca ggagtcttct gttacatgat agcggtagcc tgcttcagca
     8581 cccacataca aggaatgaga actgtagtct tttgttccga gtccagcaaa ggtcgccgag
     8641 tactcattat catggtgaac atacttacta atcaggtcga tataggcacc agagtcaaat
     8701 atagcggaag catacagacc tgccccgacg gacttcgtct tgccactgaa tgcattactg
     8761 ctggcatgac tgtcggtata cgtcatagtc agaccagtga aaaggtcaag tccatccagc
     8821 tcatgttttt tatccacacc aatctgcaca tgggtgtagt tgtcactgta tccaccagaa
     8881 gctgaacctg ctccgctcat gatgcgggcc catgcaccgg cctcgccatt ggtgtcacgc
     8941 agatccccca ttcgtttatt caggttgttg acttctgtca ggaaggcttt gtaattaccg
     9001 gacatatacg attttgctgc accggaagat gctgtatttt cagccacctt atatccggtc
     9061 agggtccacg tggtatgttc ctgatcctct ttcgtgctga tcacaggaga tatgttgctg
     9121 aaacctatgg cctgagtact ggctttaaat atctctttat tgcttccttt tggggctgtt
     9181 atcaggtcaa cgtttaatcc tgagttatta tttcccactt tctgtatatc gaccgacaga
     9241 ctgttattgc ttccggttaa ctgattttta acaatgagtt tatccgattg atttacagac
     9301 tggctcagat cagcccgcat aatcacagca gtattagtgg catccagtgt gtttactgtc
     9361 acagtatgga aacccgaatt ctctatgtta ctaaacaaca aaagactgtc gcgggttttt
     9421 aatgattgca gggctgactg tccgtttact ctccataagg tgttatacat gctggctgag
     9481 gctgcaggag catcaatacc tccggtataa gaagtatcaa agccacccag caaacctgta
     9541 gccagcggtg tgtgtgtggt tttgtccagg ttatcttctt tatactggtc aacaccaaac
     9601 agaatagatg cttttttgtc tgattttata ttaccattga cccaggcata attttgagcc
     9661 tttaacgtgg caccatcttc tgtcatatta tatccatctg acatatagat tgccgggctg
     9721 tatttattat tttctgtagg ggtgcctgtt aatgtcaggg ttccggccag ttcaatagct
     9781 ttatctacat acatttttcc ctgggcggta acagtagctc ctttgttgat aatcaggtct
     9841 gtattaatta aggataatgc accagtcata gtaacagttg atttatcacc aacagttatt
     9901 ttgctgtttt gagcactcag actgccgttg aaactacttt cgcctgctga atccccggag
     9961 cgaagctgtt gtctgaatcc aaaaccttcg ccagtaatgt ttcttcctga gaacatgtca
    10021 atgtatgcag ttttatttcc cagaacaatt tgagagtttt tagcctctat gtgtcctttt
    10081 acattagcat cccggcctat actcagggtt gcgttttcta aatttaattt ccggaaatta
    10141 aatttacgac tttcccagtc gggctgggaa aaagaggcta tctgattgtt ggacttatat
    10201 tccgtattgt aacgtttatt tacagtgctt tcctggtcct ttatggcagc agaatagtct
    10261 ttttgacata aaaaaggaat tggacagttg ttattgcctt ctttaaatat ggcatgttct
    10321 gtcgcatgtc cttgtaatgt taactgtgca ttccggacag agatatcgtt tttgatatcg
    10381 actgagccat caaagatcag attggcatta ttgttatgtt gctgggtagt attgataata
    10441 tgatttatgc ttatattacc tgatacattc ccatggtaca tatatgcatc agtattagta
    10501 agtgataatg ttgattgctt cacgtttgag ttcgttacgg ttgttcccgc atctgttgct
    10561 gcgattttct gaaatgactg gtcatagccg tttagatcca gtgttccgcc atttctggta
    10621 aaatagattt cgccattacc gctttcgctt aaagcgtctt tggcatttat ttttaccgta
    10681 cctttacctc cggccatgta aattttattg aaggcttttt cagcactaag gatgacagtc
    10741 ccattaccta ttttaaggtt atttccctgt gctattttta catccagagt accactacct
    10801 attttatgca gattatctcc ggcaacgccc ttaacattcc attcaacggt ggtattatta
    10861 tcaatatcta tcccggcacc tttataagac ttatcttttc cgtacacttt atatgtctgg
    10921 ttcttatcga atataagccc gccaatgcca agatcggtat cctgcttgag cgttaatacc
    10981 cctcctcctg agacagacag gtccttatct tttataatcg atgataactc ttgtttttta
    11041 ccatccagct gcatagtgtt tgtgaaattc ttttttaaat tgtttaccag aatattatta
    11101 aataacgagt agtttgtcca ggctatattg gtactactga taaaaccaac tgtactggta
    11161 acaccatgga aaacccattt ttgttgttct ttatcatata gataagaacc agaaccacta
    11221 tcgccaaaaa ttggtgagtt tttaaaaggg gttctgttgg tataaatgta actcttgttt
    11281 ttccagtcca gattaaaaag acttgcgctt aacaagtcgg gattatagtt ttgtccaaac
    11341 agatattgtt tgccgtttaa tatcgttgag gttgttccgg cacctgctct gaagccaatt
    11401 atttgttcct taccgttgta gtttacgcca tatcttttca tcgcatcttc gggagaaagt
    11461 gagaagtcgg cactctccgt aacccctgtt gtttccacga cgaatttatt cagacgatga
    11521 accgaaaagt cgccagatga tactctgtta cttgctttat atgctgtctg atcccatgac
    11581 tgtgttgtta ttgcatgatg ctgtgtaccg ttatgagtcg cagtaacact gtacgcacct
    11641 ccaattgatg ttgttgcacc tttgttggaa acagcagaaa aatcaggtac aggtagttct
    11701 ggaaaatgaa atatctttcc atctttcatc attaattgaa catttgttgc acccggtctg
    11761 aactctcctc tgttttgagc aaggtcaaga taatcgcggg tccatatatt atttatatcc
    11821 agcattgctg caaatgctgt ttcgccataa cttccatata ttgaggataa aataataccg
    11881 aggataactt tctttcttga tgtaccgaca ctaactttcc gggtcagttc agaaacagca
    11941 actaatccac ctgtaatatg actatatttc agtgaataaa ttttattcat acgtgaactc
    12001 cgatatttct gagttgtggt tatgggatag catttaatta caacggggaa ctatcagtca
    12061 tggtacgtaa ctgataaggg tataatgata atcactgtca gttatatttg tatgacagat
    12121 gtaacaaaat gaaaaaaaag ataagcacaa gtgattgatt ggttagatat tgatcacttg
    12181 gtgtttgtat tttgaatttt gcgatcaaaa tttgatcttt gttgtttccg gtcaatcctt
    12241 tacaggtttt ctgtattatc cgggggagtt aagtctgtcg atgtccgaca gacttttgat
    12301 gatggaaaca acatgcagcg ataatgtgca tgctgttttg caggtggaac tcagaaggtg
    12361 aactggaact gtgcgaccgc accgtaagtg ttgtccgtgc cgatcaggcc tgcaacatca
    12421 aggtgaacca catcaaacgg tgagatcccc accccggccg tgaatgcact gccgtcgtta
    12481 ccggccagat tctgacggta accggcgcgc agctgtgccc atttccaggc attaaattct
    12541 gtgccaatgg cggcaaactg acggttgctg tcggaggtga aaccgctggc cggggtcaga
    12601 tccacatcaa atgcagtggt gaacatcgcg ttgtgccagg agacaccggc cgtcacctgc
    12661 gggcggatcc tgaaggtttc cgtgataccg ttcacttctt ttgtctcaat actacgggag
    12721 atcaggttct gtgcgaccag tcccagcgtc cagttgtcat ccaggtccgt actggcaccg
    12781 atgtcggcgt tgatgccatt tttcgtgttg tggtagcggt cgcctttgaa ggcgctgctg
    12841 tcataatttc tgaccagcac gttgtagttg aacaggtcga cgcgctggaa tttggggggg
    12901 aagcccagag accatttctg cccggccgtt tccagttccg tagccagagc aatacccaca
    12961 tcagtgatca gtgccgtgcg ggcacaggcc tgtgatgtca ggcggctttt gtccacctcc
    13021 tgcccggtgc tgtttgccac ctcttccagg taagtgaggt cagcatcaga gactttaccg
    13081 tcgacactga cggtgccgtg tgatttaacc atcagtgcgg cagagaggcg atcaccggga
    13141 agggccgcca cagcagaaac gcccagctgt gcggcggcat gtgtgttacg gaagtcccgg
    13201 aggtgttctt tcaggcgtgc ggcagcctca ggaacaccgt ggttactttc gatggcgcgg
    13261 tcgaaggcct tccactcatc actgatccgg tcggcgttgt cggtgatatt atccggattg
    13321 gagagctgcg cgccgacgga cggaagcagg atgctgggct catgcgcgac gcaaaatcca
    13381 cgatgtatat atcagtacta aaagcgcgac ggcggaagaa gctctgaaac taatcggtga
    13441 actgtacgcc atagagcacg atatacgcgg gttaccggtg tctgaacgcc tggcggtcag
    13501 gcaaatgcag agtagaccgc tcctgacttc cctgtataag ctgatgcagg agaaagaaca
    13561 cacgttatcg aagaaatgtc gtctgggaga tgcgttccgg tatatcagga agcactgggc
    13621 tgcgttgtgc aacttctgtg atgatggcct ggctgaggta gataataatg cagcagaaag
    13681 agcgcttcgt gcagtctgtc tcgggaagaa aactttttcg aggatcactc attccgtgtc
    13741 tgactgcttc agggtttaat tttgcttctg ctgactgaat gatgattggg cagcaagtgc
    13801 aatatcccac ataaatccag tcaactcacg agccaccgcc gtaatggcta cttgtgattt
    13861 tttccctttt gctgtcagag ttcggtaacg agaacacaat cgttgttgag ctttccatgc
    13921 aatatctttg gaatattgtg ttacagagtc cggtcggtaa tatataagcc atgctccaac
    13981 ttttgcaggt gtacgataag accaggcggc ttcgtaaagc agacgtctca gttcgctgtt
    14041 tccaactttt gttattcctc tgggacgaat actgtttcca ctggaatatt cacctggcac
    14101 ca
//
LOCUS       DAZOYF010000103        14093 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZOYF010000103 DAZOYF010000000
VERSION     DAZOYF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.103"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..455
                     /locus_tag="ACHUOF_002815"
                     /pseudo
     CDS             1..455
                     /locus_tag="ACHUOF_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            515..622
                     /locus_tag="ACHUOF_002816"
     CDS             515..622
                     /locus_tag="ACHUOF_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR0611919.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(603..1334)
                     /gene="yehW"
                     /locus_tag="ACHUOF_002817"
     CDS             complement(603..1334)
                     /gene="yehW"
                     /locus_tag="ACHUOF_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR0611920.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1339..2265)
                     /gene="yehX"
                     /locus_tag="ACHUOF_002818"
     CDS             complement(1339..2265)
                     /gene="yehX"
                     /locus_tag="ACHUOF_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR0611921.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(2258..3415)
                     /gene="yehY"
                     /locus_tag="ACHUOF_002819"
     CDS             complement(2258..3415)
                     /gene="yehY"
                     /locus_tag="ACHUOF_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR0611922.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="ACHUOF_002820"
     CDS             complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="ACHUOF_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR0611923.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="ACHUOF_002821"
     CDS             complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="ACHUOF_002821"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR0611924.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7084..8799
                     /gene="dld"
                     /locus_tag="ACHUOF_002822"
     CDS             7084..8799
                     /gene="dld"
                     /locus_tag="ACHUOF_002822"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0611925.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="ACHUOF_002823"
     CDS             complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="ACHUOF_002823"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR0611926.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9943..10530)
                     /locus_tag="ACHUOF_002824"
     CDS             complement(9943..10530)
                     /locus_tag="ACHUOF_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR0611927.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10700..11278
                     /locus_tag="ACHUOF_002825"
     CDS             10700..11278
                     /locus_tag="ACHUOF_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0611928.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11395..12117)
                     /locus_tag="ACHUOF_002826"
                     /pseudo
     CDS             complement(11395..12117)
                     /locus_tag="ACHUOF_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12209..13645)
                     /gene="mdtQ"
                     /locus_tag="ACHUOF_002827"
                     /pseudo
     CDS             complement(12209..13645)
                     /gene="mdtQ"
                     /locus_tag="ACHUOF_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            13869..13952
                     /gene="yohP"
                     /locus_tag="ACHUOF_002828"
     CDS             13869..13952
                     /gene="yohP"
                     /locus_tag="ACHUOF_002828"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR0611929.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3354 a         3937 c         3550 g         3252 t
ORIGIN      
        1 cggcaatcta catagcctgc gaacgtggat caaagagcgc ggtcaggatt accccgccca
       61 gacactcacc acacatctgt ttattcctct gcgccgacgg cttcagtgcc aacaaccgac
      121 tctccaggcg ctgctggcga tcctcgacgg cgtactgatc aactacatcg ccatttgtct
      181 ggcttcggca cgtaaaaaac agggtaaaga tgcgctggtg gttggctgga atattcagga
      241 taccacccgt ctgtggctgg agggctggat tgccagtcaa caaggatggc gcattgatgt
      301 cctcgctcgc ccactcgctc aatcaactac gccctgaact attcgaaggc cgtacattgc
      361 tggtgtggtg cggtgaaaat cgaacctccg cccaacagca gcaactcacc agttggcaag
      421 aacaaggcca tgatattttc ccactcggta tttaatgatt cgttaacaaa tgcgctttac
      481 tgtacaatcc tttcgttaac ataaggagtg cattatgcgc atagctaaaa ttggggtcat
      541 cgccctgttc ctgtttatgg cgttaggcgg aattggtggc gtcatgctcg caggttatac
      601 ctttattttg cgtgctggct aagcgcctgc accagccttt caaacaggcg gtctgcgatg
      661 atcgccgcca gtgccaccag taacgccccc tggatcacat acgcggtatt aaatccgcta
      721 agcccgatga tgatgggcgt acccagcgta ctggccccta ccgttgaggc gatcgtcgcc
      781 gtaccaatgt tgataatcac cgaagttcgc acgcccgcca gaatcaccgg agccgccagc
      841 ggtagctcga ccttacgcag tcgctgacct cgactcattc ccataccttt cgcaacttct
      901 gtcacgctgg gatcaatcgc tcccagcccg gcaagtgtca cctgcaggat gggcagcaca
      961 ccgtaaagga tcaaggcgat aatcgctggt tgcagaccaa agccgatcac cggaacggca
     1021 atcgccagca ctgcgacagg cggaaaagtc tgtccaacgg cggcaatagt ttccaccagt
     1081 gggcgaaatt ccgcgcccca cgggcgagta acagcaattc cggcacctgt gccaatgatc
     1141 accgcaaaca aactcgaaat tcccaccagc cagaaatgag ccagtgccag agctgcaaaa
     1201 ctttcttgct gataaacggg tcgtggcagt tgtgggaaca aggcagcaaa cagcggctgg
     1261 ctgtaaggca gccagaaaat cagcgccaca aaaagagcaa tgagccagaa cagcggatcg
     1321 cgcaacatct tcatacgctt acgcctccac cagcagatcc tgaaaatgca gcgtgccgca
     1381 aggctggccc tgcgtgttca ccaccggcag cacctcgcat ccccgcgcga caaacaggga
     1441 gagcgcatcg cgtagcgtca tctcttctgc cagtgcctca ccttctgccc gttcttcgcg
     1501 acgcacgtaa tccgccacgc tacgtaacga aagcaggtgc acccccagtt cactacgtcc
     1561 aaaaaactgg cggacaaaat cattcgccgg acgagtcagc atcgtcagcg gattcccctg
     1621 ctgcaccact tcaccgtgat ccatcaatac cagatgttct gccagccgta gcgcctcatc
     1681 aatatcatga gtgaccagca caatagtgcg ccccagcaaa cggtgaatgc gcgtcatctc
     1741 ttgttgcaac gcgccgcgcg ttaccgggtc cagtgcgcca aaaggttcat ccatcagtaa
     1801 gacttgcgga tcggcagcca gtgcacgcgc cactcccaca cgttgctgct gaccaccgga
     1861 aagctgatgc ggataacgct cacgcaaatt tgactccagc cccagtagcg ccattaattc
     1921 gtcgatacga tcgtcaatcc gcgcccgcga ccatttttgt aattgcggca cggtagcgat
     1981 gttttgcgcc acgctccagt gggggaacag gccaatagat tgaatggcat agcccatccg
     2041 gcggcgcaac tccagtactg gcagcgagcg aatttcttct ccggcaaagc ggatcactcc
     2101 gctgtcatgc tccaccaggc ggttaatcat tttcagggtg gtggatttgc cggagccaga
     2161 tgtgccaatc agcaccgaaa aactcccttc ctgaaaattg agattgagat cgttaacggc
     2221 tttttgtgcg ccgaacagtt tgctgacatg gctaaattca atcattacgt ttcaccttca
     2281 gcagtgcgat aagcaaatcg aacagcgcgt cggtaagcac cgccagaaca attaccggga
     2341 tcacccccag caacactaaa tcaatggcgc tgcttagcag cccctggaaa accagcgcac
     2401 caaaaccgcc tgcgccgatt aacgccgcaa tcaccgccat acctacagtt tgcaccatca
     2461 ccacccgcag gctgcgcaga aataccggta acgccagcgg taactgaaca tgcaggaaac
     2521 gctgcgcccc gctcatcccc atcgctctgg cgctctccag cacatcgcgc gggatctggt
     2581 tcaaaccgac taccacgcct cgcaccagcg gcagcaaggc atagagcacc agcgcaatca
     2641 gtgcgggtgt cattccggtt cctgctatgc cgagcttccc cagccacgga aaggccgtaa
     2701 ccagcgcggc aagcggcgca atcaacaggc caaagagcgc caccgaaggc acggtctgaa
     2761 tgacattgag cagagaaaaa attgccccct gccgcgcagt ggaaaagtag caccagatgc
     2821 ccaacggcac accaatcact aacgcaggca gcaccgcacc aaacagcaac gtcagatgtt
     2881 gtgccagcgc gtcgtcaaac acatcctgac ggttggcgta ttctttcatt agtgagagat
     2941 cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca
     3001 accagcgcca cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg
     3061 ccagcgcagc agccagccag aaacctctgc cgaggctggt acgcgccagc gcactgccat
     3121 tttgcgccag ttgggtcgcc gcctttccag ctccccacac caacaatacg aagacgaatt
     3181 gcgccagaat gagtgcaaaa atgctgcctt ttttagcggg gacaaaacag gctgtcagcc
     3241 aggcgcaacc aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgaa
     3301 gcccttcacc cgagactaaa cgattaggcg cgtaactgat aaacggcagc gccgctgcga
     3361 ttgccgtcag caacaacagc agcgccagaa caggattaat acgtagataa gtcacgggaa
     3421 attacttcgc ccaccctttt tgtttcaggt agtcggcagc cacttttttg gcatccagtc
     3481 cttccactgc aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga
     3541 ctggctgtag ccactgtgcc atttgcggat actccttcaa caccgattca cgcaccactg
     3601 gtgcaggcgc gtagataggt tgcacgcctt gcggatcgct taaggtttgc agccccagcg
     3661 ccgcgaccgg accgtcagtg ccgtaagcca ttgcggcatt aacgccagag gtttgctggg
     3721 cagcggcttt aatcgtcacc gccgtgtcgc caccagccag tgacagcaac tgatcctgac
     3781 cgagcttaaa gccataggct ttttcaaacg tgggtaacgc atcggcgcgt tcgataaact
     3841 ctgccgaggc tgccagtttg aaggtgccgc cctcttgcag ataacgactc aggtcggcaa
     3901 gcgaagtgag tttgtttttc tctgccacat cctgacgcac ggcgatagtc caggtgttat
     3961 ttgcaggcgc gggcgtcagc cagattaact tgttgtgctc cgcatcgagc tttttgactt
     4021 tctcgtagcc ctgcccggca tttttccacg ctgcatcgtt ttcatcttta aagaagaaag
     4081 cgccattgcc ggtatattcc ggatagatat ccagttcatc ggaagtaatc gccccgcgcc
     4141 ccacaggagt cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg ctttcgagta
     4201 cctgcaagat gatgttgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa
     4261 cgggggaagc cgcctgtagc ggcaggctca cggctgccaa cataaccagt gaacctgccc
     4321 agagctttaa gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta
     4381 agagaaaagc atagttgata atgacagagt tagcctgagc ggaaggattc agttgcagaa
     4441 tcagataaat acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc
     4501 gccttatccg gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa
     4561 gggcgcaaac gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac
     4621 gcgtgcggaa tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat
     4681 ctgttgattc cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc
     4741 gcccggtttc agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga
     4801 agccgtcaca tcctgcaagt acatctgcac taccgtggcc ccctcgcgct taccggtgtt
     4861 cgtcacctgc acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt
     4921 cacatcagag acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgctcc
     4981 gttagcttca tcaaaataac gcgaagtata tttgttcggc ttgtcggcat tatacggacg
     5041 accggtgttc agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggaaat
     5101 tggcagcttg ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc
     5161 ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag
     5221 cgccagcgga cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc
     5281 cgcaatcaag tcacgttggc cttgcggaat agtgatatcg gtccggctgg aggcttcgtg
     5341 cgccatcccc tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg
     5401 caccgcttca tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata
     5461 ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag
     5521 cactttaccg ttttcaccga cggaattttt aatcccggtc agtacggtca cggattgatc
     5581 ggcaacaccg gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc
     5641 aaccaccgca atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa
     5701 caccaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt
     5761 ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaaccc
     5821 catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatggtcac
     5881 tttgccggat ttaatcaacc caggcagata cttcgagtag tactcgtcgc tcatactcat
     5941 gttgattccg gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg
     6001 tttaatcagc tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg
     6061 gtcgcgcaga acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga
     6121 gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat
     6181 ataatcatta aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc
     6241 taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg
     6301 gcttttaccc tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc
     6361 ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg
     6421 tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac gcccgaccgt
     6481 tttcaccgca tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg
     6541 ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat
     6601 cacctgatcc tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac
     6661 ctgaccgtct ttgatcatct cgcggatcgc ctctttcgga ttatccgggc ctacgctgat
     6721 taagcgcagc tgaccaattt tctcatcaac cgtcattttc ttgagcagat cggtgacgaa
     6781 cgcatcccgc gcttcaggcg ttaatggatg gttaccgaat aaatcatccg ccagtgctgg
     6841 ctgcaggacc agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct
     6901 tcctttgttg cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat
     6961 gatggagaga gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct
     7021 gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg
     7081 aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg
     7141 gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc
     7201 cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg
     7261 cgggtgctga aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca
     7321 ggcctgaccg aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc
     7381 agcaccctgc gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat
     7441 ccgggcacca cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcat
     7501 tcggtgattg gatcatcgtg tataggcgca tcggtcatcg gcggtatttg taacaactcc
     7561 ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata
     7621 gatgaagatg gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg
     7681 gagcagatcc ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat
     7741 ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg
     7801 cccgcacgtt ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag
     7861 ctggccgtct ttgcggtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt
     7921 tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac
     7981 ttcgaaaatc tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa
     8041 aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc
     8101 ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg
     8161 cattttactg accgtgcaat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg
     8221 cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat ggcaggcgat
     8281 ggcgtcggtg aagccaaatc gtggctggtg gattatttca aacaggccga gggcgatttc
     8341 tttgtctgta cgccggagga aggtagcaaa gcgtttttac accgtttcgc cgctgcgggc
     8401 gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcactggat
     8461 atcgctctgc ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc
     8521 cagctggtgc acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac
     8581 atagtgaaaa aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag
     8641 cagcgcggcg cgcagtaccc tgccgagcat aacgtcggtc atttgtataa agcaccggag
     8701 acgttgcaga agttctatcg cgagaatgat ccgaccaata gcatgaatcc ggggatcggt
     8761 aaaaccagta aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat
     8821 cgccatccga attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg
     8881 ctttttgttt tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa
     8941 tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca
     9001 ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg
     9061 cattggtaaa gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat
     9121 tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg
     9181 tagtacttaa ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt
     9241 cacgggcagt tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt
     9301 tcattccgag cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg
     9361 gataatggtg cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca
     9421 gcatatcttt acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct
     9481 ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca
     9541 caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt
     9601 tcgaatagat cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt
     9661 ccggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gcaaaaggca
     9721 cagccagcat cagggccaag ctaaataaag aaactcgaaa tttcggcatg atgagcattc
     9781 agatagtggt tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc
     9841 gacgccatca taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag taaatgctgc
     9901 attgctgaca tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc
     9961 gtagccccat agtataacgg ttagggtgag cagcacttcc agtaccagca cgccaatcgc
    10021 cagcgtcgaa ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat
    10081 accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa
    10141 ccagaccagt ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc
    10201 cgcgaagacc atgcagtgcg caagagacgg acgctgcggg taattgcgcg ccatccacca
    10261 gatgacccgc cccatcaccg caaccccagc cagcataacg ccgtaaaaca agacagccag
    10321 cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat
    10381 ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg
    10441 ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg
    10501 atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct
    10561 gacagttcaa gtataagcca gcttgtgatt attttttgtt catccatgta atttgcaccg
    10621 tctgatgtat gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca
    10681 aagggagaca aactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc
    10741 gctggttatc ggtagcctgg cggaaggtga aaccgtgact ttgctgggag gcgttgcggc
    10801 acatcagggg ctattaaagt tcccactggt ggtactttct gtggcgcttg gtggcatgat
    10861 tggcgaccag gtgctctatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt
    10921 ctcgaaacat caggataaaa ttgagcgggc acagaaactt atccaacgcc atccgtatct
    10981 gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg
    11041 tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg
    11101 ggcgttgatt tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca
    11161 caatctcgac cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt
    11221 gggcgtgcgc tggtggctga aacggcgcgg gaagaaaaag ccggataatc aggcgtaaag
    11281 cagttgccac agataagcat tcactccaca tccggcaccg ggtgttccat gcctgatgcg
    11341 acgctggtgt cttatctggc ctaacccatt gaaatatcca cgcacctcac tgcgctattc
    11401 tgggttgaac tgcggattcg ccaacataaa gccgccatcc actatcaacg actgccctgt
    11461 cgtgtaattt gcgccctccg aacaaagcca caccaccagg ctggcaatct catgcgttgc
    11521 gccaaaacgc cgtagaggaa tcgaaggctc cgcgtcgggc ttcacgtcgc tatcatccat
    11581 gctgttcatt ggcgtggcta tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct
    11641 gaccaactcc agcgccatcg ttttggttaa cccgccgagc gcatgtttag cggctgtgta
    11701 ggcgctggca tccggcagcg gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc
    11761 gccctgccct tatttcacca tctgacgagc cgcaatttgc gagcataaaa atgcaccatc
    11821 aacatcaacg gtaaaaatct tgcgccactc atcaaaagcc atatcaagaa acggcgcttt
    11881 ggtcattgca cccgcattat tcaccagcac atcaatacgc ccaaatcgct ggatgagtgt
    11941 ctccagcgcc tgcgcacctt ctggcagatt gccgagatcc agttgcacga tctccgcacg
    12001 tacgccgtgg gtaaccacct cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca
    12061 ggtaatgcca atatcaaacc cctgctgcgc cagtaataac gcgcactctt tgccgatccc
    12121 cgaatcggaa gcggtaatta tcgcgaccta tgccatcgag ttctccactt aacgctgaat
    12181 aaacgttaag tatagaaggc gcatatcatc agcgtttgta acccccgccc agcgcaccgg
    12241 tgagtcgaat ggaggcatcc agccactgcc cttgcaataa caggccattg gcccgctcac
    12301 gcagcgccgg gattctggct tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc
    12361 gggcctgcgc aagacctacc acacgcaagg catcgcgctc aatttgcgcc tgatgctggt
    12421 ttttctccgc cagtgtctga acctgactgg ctgcccgcgc cacgtcattc actgcttcaa
    12481 ccaccgcttt gttgtagctg gcgatagaca agttgctttc ggcttttgcg atatcgagat
    12541 tggcgttaag acgaccacta tcgaaaatag gtagcgtcag gcctgccgta acgcccattt
    12601 gctgcgcgga atgacggaac agatcgctta agtgcaacgc atcctgttgc aggaaggcca
    12661 tcaggttgat gtcaggataa aatgccgctt ttgccgcatc aatagtgctt aacgatgact
    12721 caacgtacca gtgcgccgcc tgcaaatctg cccgccgggc cagtaaggag taccccagtt
    12781 catcaggaag ctggcttgcc actttcggca acgcgaccgg tttaagcttc aatgacttag
    12841 tctggtgatt tgtgagtgca tttaaccgtg cctcaataat tttcattttc cccgcgacat
    12901 cgttgagctg ctgccgggtt ttgctggcat taatatcggt ttccacaccg tcaactgaag
    12961 aagtaatccc gttctgatat agctggcgat cggtcgcgat aatggtgttc tgctcttttt
    13021 ctatttgctg caagaccgtg tttaacgccg cctgggtttg ccactcccag tacaggcggg
    13081 ctacgctgcc agccagcaat tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg
    13141 tacccaggcg ggcaataacc tccgcccgat tctttcccca gatatcgaga tgccagcccg
    13201 ccgttaagcc aaaagttccg ttggtgtacc acggaccggt cgtacctgcg gccggatcgt
    13261 tcagagcaaa cggccccatt agcccttctg ccgacatttt ttgccgctcc atatccgccg
    13321 aaaagtcgat ctgcggacca tcctgagtgg caactgcctt cgcctgggct tcagctagct
    13381 aaatgcgctg ttcagccacc tgcatatccg gtgcgttctg tagtgcattg ttaattaatg
    13441 aagtgagctg attatcgtga tactccagcc accattggct gtctggccaa ccatttttca
    13501 gcgccgtggg taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc
    13561 gggtttcatg cataggcgca cacccggcca gcatcagtaa cagcggaaaa caggcgatgg
    13621 ctggataaaa ggaatcacga ttcatggggg aataatcagg taagaaacgg tgcgcggaga
    13681 ttaccgtgtg ttgcgatata ttttttagtt tcgcgtggca atacatcagt agcaataaaa
    13741 cgacataccc agaaaaatat agactaagta aatgatatct tccgatttat cttaatcgtt
    13801 tatggataac ggcaaagggc ttcgtttttt cctatactta ttcagcactc acaaataaag
    13861 gaacgccaat gaaaattata ctctgggctg tattgattat tttcctgatt gggctactgg
    13921 tggtgactgg cgtatttaag atgatatttt aaaattaatt aatgtcatca gatccgaaaa
    13981 taacgagaat atttcagtct ctcatcctgt tgcgctcctg tcatgtgcat tgcttcatat
    14041 aatcactggc gcaaggagcg cgcagggggc gaccaatcgc cgccgccccc tgc
//
LOCUS       DAZOYF010000104        13991 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZOYF010000104 DAZOYF010000000
VERSION     DAZOYF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.104"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..286)
                     /locus_tag="ACHUOF_002829"
     CDS             complement(<1..286)
                     /locus_tag="ACHUOF_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR0611930.1"
                     /translation="MLARDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFY"
     gene            complement(296..847)
                     /gene="ybcL"
                     /locus_tag="ACHUOF_002830"
     CDS             complement(296..847)
                     /gene="ybcL"
                     /locus_tag="ACHUOF_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR0611931.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1016..1348)
                     /gene="emrE"
                     /locus_tag="ACHUOF_002831"
     CDS             complement(1016..1348)
                     /gene="emrE"
                     /locus_tag="ACHUOF_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR0611932.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1693..2007)
                     /gene="fliE"
                     /locus_tag="ACHUOF_002832"
     CDS             complement(1693..2007)
                     /gene="fliE"
                     /locus_tag="ACHUOF_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR0611933.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2221..3880
                     /gene="fliF"
                     /locus_tag="ACHUOF_002833"
                     /pseudo
     CDS             2221..3880
                     /gene="fliF"
                     /locus_tag="ACHUOF_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            3873..4868
                     /gene="fliG"
                     /locus_tag="ACHUOF_002834"
     CDS             3873..4868
                     /gene="fliG"
                     /locus_tag="ACHUOF_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR0611934.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4861..5547
                     /gene="fliH"
                     /locus_tag="ACHUOF_002835"
     CDS             4861..5547
                     /gene="fliH"
                     /locus_tag="ACHUOF_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR0611935.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5547..6920
                     /gene="fliI"
                     /locus_tag="ACHUOF_002836"
     CDS             5547..6920
                     /gene="fliI"
                     /locus_tag="ACHUOF_002836"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR0611936.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6942..7283
                     /gene="fliJ"
                     /locus_tag="ACHUOF_002837"
                     /pseudo
     CDS             6942..7283
                     /gene="fliJ"
                     /locus_tag="ACHUOF_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
     gene            7280..8407
                     /locus_tag="ACHUOF_002838"
     CDS             7280..8407
                     /locus_tag="ACHUOF_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR0611937.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8512..8976
                     /gene="fliL"
                     /locus_tag="ACHUOF_002839"
     CDS             8512..8976
                     /gene="fliL"
                     /locus_tag="ACHUOF_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR0611938.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8981..9985
                     /gene="fliM"
                     /locus_tag="ACHUOF_002840"
     CDS             8981..9985
                     /gene="fliM"
                     /locus_tag="ACHUOF_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR0611939.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9982..10395
                     /gene="fliN"
                     /locus_tag="ACHUOF_002841"
     CDS             9982..10395
                     /gene="fliN"
                     /locus_tag="ACHUOF_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR0611940.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10398..10763
                     /gene="fliO"
                     /locus_tag="ACHUOF_002842"
     CDS             10398..10763
                     /gene="fliO"
                     /locus_tag="ACHUOF_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR0611941.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10763..11528
                     /gene="fliP"
                     /locus_tag="ACHUOF_002843"
                     /pseudo
     CDS             10763..11528
                     /gene="fliP"
                     /locus_tag="ACHUOF_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            11538..11807
                     /gene="fliQ"
                     /locus_tag="ACHUOF_002844"
     CDS             11538..11807
                     /gene="fliQ"
                     /locus_tag="ACHUOF_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR0611942.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11816..12601
                     /gene="fliR"
                     /locus_tag="ACHUOF_002845"
     CDS             11816..12601
                     /gene="fliR"
                     /locus_tag="ACHUOF_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR0611943.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            12886..13510
                     /gene="rcsA"
                     /locus_tag="ACHUOF_002846"
                     /pseudo
     CDS             12886..13510
                     /gene="rcsA"
                     /locus_tag="ACHUOF_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
     gene            complement(13554..13742)
                     /gene="dsrB"
                     /locus_tag="ACHUOF_002847"
     CDS             complement(13554..13742)
                     /gene="dsrB"
                     /locus_tag="ACHUOF_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR0611944.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13905..>13991
                     /gene="yodD"
                     /locus_tag="ACHUOF_002848"
     CDS             13905..>13991
                     /gene="yodD"
                     /locus_tag="ACHUOF_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR0611945.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEI"
BASE COUNT         3497 a         3593 c         3612 g         3289 t
ORIGIN      
        1 cataaaattt ttgaaacttt tcaattagat caatgggata ataaagcatt tctgcaagat
       61 aaagacctgc ttctggataa ttcacaatat aaaattcata tccacaggga aataatatta
      121 tctgattatt atcaacagtt agactatgcg tctcccaatt gataactttt tttccctgac
      181 ggatacgaca taaagctggc ataagaggct taaccctgtg aatctcatga tgtttatgca
      241 tccgtatttc ttcaatcttt aggttagact tatctcttgc cagcatactc ttaccctatt
      301 ttatctcata aactggtgtt atctcagcgg ttgcaatttt attggcatta agcatataac
      361 caactaacgc tccgctggaa ttagaatcca caggaatctt atcagttttt agagcccata
      421 ctttaaactg gtaatgatgg ggtttatctc ctttgggagg acatgctcca ccaaatcctg
      481 cataaccaaa atcatttcgg ccttgaacag caccagtcgg aagttttgta ccatcacgtc
      541 ttcctgcatc agtgggcaaa tatgttacag ttgctggaat attagcaaca gtccagtgcc
      601 accatccact gcctgtagga gcatctggat catacacagt tacggcaaag cttttggtgc
      661 cttcaggagc accagaccag gttaatgagg gcgatgtatt accaccttca cacccaaatc
      721 cagaaaatac atgagacgtt gtaagttgct ctcctgtttt tatttcatta ctagtgacct
      781 gaaatgctgc ggcctgcgca gaaaatgtta tgaatgccaa tacacttgaa acgataagtt
      841 ttttcataaa aacctcattg ttgtgaccta tcgttatttt atttgatgtg ccttgatctc
      901 attatgcata aaggcgcaat gatcgcgcaa atgcaatcat aattataaag gtgccccgaa
      961 cattagccat aatatgcaca aatcagattc ctgttatctc aaggagaatt attttttaat
     1021 gcggtgcact tcgtgacaac aagttaatga ctaacacacc ggcacaaatc aacatcatgc
     1081 ctatgatagc tggcaggtcc agtcgttgac caaaaaatcc ccaggaaagc aaactaatca
     1141 gaacgatacc gactcctgac cagatagcat aagcaatccc tgtaggtata taagccagcg
     1201 tctgagctaa taaccagaat gatgcacaat agcaaataat tgtgccaaca gagggccata
     1261 accgtgtaaa cccctctgaa aacttcatta aggttgtacc aatgacctct gcaagtattg
     1321 cgccaccaag ataaatataa gggttcatag catattcttt cctgttcata ctggagagaa
     1381 ttgtactaca gtttgaacac aactcaccgt tttcatcctg gtgtatgtgt atccaagggg
     1441 ctctggcgtg gcaagagata aaggcaggaa caataatgat cagaaggaac cccaggctaa
     1501 cgaatgagtt cactcttcct gggagcaaag cgaagacaaa acatgttatc gcgtttacgg
     1561 atcgtggatg gcagaaaaca accaggatca ggtatccatt ttaaaccaga aattgagtga
     1621 gtttgcccca tccatgcccc acgctgtggg atcggatata attaaacaag catagtcaac
     1681 aggttacaca acctacacct gcatggacat cacttcctga tacgccgcca ccagcttatt
     1741 acgcacctga atccccattt gcatagaaac tgaggctttt tgcatatcgg tcatcacatc
     1801 gtttagcgcc acgccgggtt caccgagggt gaatttttct gcctgcgtgc gggcgactgt
     1861 ttgtgtatcg cttatgcgat cgagcgcggc gtgcagctgc ccggcaaaac taatggtcgg
     1921 ttgcggcagt gattcctgcg cacgcgcact catcgccgta gcctgtaact ggctgataac
     1981 cccttcaatc ccctgtatcg ctgacatttt cgtctcccgg ataatttctg gtaacaaagc
     2041 ctaccagtaa gtcaataaga caaaggcgct aaatagcaac aaaaaaactg gtttattggc
     2101 ggatagaaaa aacgaaagca caaataatgg gagcgtcaat ttttcgagtg tgctgacccg
     2161 agagtgagtc ttgttccact ttgccaataa cgccgtccat aatcagccac gaagtgcgcg
     2221 atgaatgcga ctgcagccca gacaaaatct cttgagtggc ttaatcgcct gcgtgcgaat
     2281 ccgaaaattc cattgattgt taccggttcc gcggcagtgg cggtcatggt cgcactgatc
     2341 ctgtgggcga aagcccccga ctaccgcaca ttattcagca atctttccga tcaggatggt
     2401 ggcgcaattg tcagccaact gacgcaaatg aatattcctt accgcttcag cgaagccagc
     2461 ggcgctattg aggttccggc agataaagtt cacgaactgc gcctggcgca acaaggtttg
     2521 cccaaaggcg gcgcggtcgg tttcgaactg ctcgatcagg aaaagtttgg tatcagccag
     2581 ttcagcgaac aggtgaatta tcagcgggcg ctggaaggcg agctttctcg taccatcgaa
     2641 actatcggcc cggtaaaagg ggcgcgcgta catctggcaa tgccgaaacc gtctttattc
     2701 gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa atctgttacc cggccgcgca
     2761 ctcgatgaag ggcaaattag cgctattgta catctggttt ccagcgcggt tgctggtctg
     2821 ccgcctggaa acgtcacgct ggtggatcag ggcgggcatc tgttaaccca gtccaatacc
     2881 agcgggcgcg atcttaatga cgctcagttg aaatatgcca gcgatgtcga aggccgtatt
     2941 cagcagcgta ttgaagcgat cctgtcacct attgttggta acggtaatat tcacgcctag
     3001 gtcacggcgc aactggactt cgccagtaaa gaacaaacgg aagaacagta tcgccctaac
     3061 ggtgatgaat ctcatgcggc gcttcgttca cgccagctta atgagagcga gcaaagcggt
     3121 tccggttatc cgggcggcgt accgggggcg ttgtcgaatc aaccggcacc tgcgaataac
     3181 gcgccaatca gcacgcctcc gacaaatcaa aataaccgcc agcagcaggc gagcaccacc
     3241 agcaatagtg ggccgcgtag cacacagcgg aatgaaacca gtaactacga agtcgatcgc
     3301 accattcgtc ataccaaaat gaacgtgggc gatgtgcaac gtctgtcagt cgcggtagtg
     3361 gtgaattaca aaaccttgcc agacggtaaa ccattgcctc tcagcaacga acagatgaag
     3421 caaattgaag ctctgacccg cgaggcgatg ggcttttctg aaaaacgcgg tgattcgctc
     3481 aatgttgtta actcgccgtt caatagcagt gacgaaagcg gcggagcact gccgttttgg
     3541 caacagcaag tgtttatcga tcagttgctg gctgccggtc gctggttgct ggttgctggt
     3601 actgctggtg gcgtggttgc tgtggcggaa agcggtacgc ccgcagctaa cacgtcgcgc
     3661 cgaggcggtg aagactgtgc agcaacaggc gcaggcccgc gaggaagtgg aagatgcggt
     3721 ggaagtccgt ctgagcaaag acgaacaact ccaacaacgg cgcgctaacc aacgactggg
     3781 ggcagaagtc atgagccagc gtatccgtga aatgtctgat aacgatccgc gcgtggtggc
     3841 gctggtcatt cgccagtgga taaataacga tcatgagtaa cctgacaggc accgataaaa
     3901 gcgtcatcct gctgatgacc attggcgaag accgggcggc agaggtgttc aagcacctct
     3961 cccagcgcga agtgcaaacc ctgagcgctg caatggcgaa cgtcacgcag atctccaaca
     4021 agcagctaac cgatgtgctg gcggagtttg agcaagaagc tgaacagttt gctgcactga
     4081 atatcaacgc caacgattat ctgcgttcgg tattggtcaa agctctgggt gaagaacgtg
     4141 ccgccagcct gctggaagat attctcgaaa cccgcgatac cgccagcggt attgaaacgc
     4201 tcaactttat ggagccgcag agcgccgccg atctgattcg cgatgagcat ccgcaaatta
     4261 tcgccaccat tctggtgcat ctgaggcgcg cccaggccgc cgatattctg gcgttgttcg
     4321 atgaacgtct gcgccacgac gtgatgttgc gtatcgccac ctttggcggc gtgcagccag
     4381 ccgcgctggc cgaactgacc gaagtactga atggcttgct cgacggtcag aatctcaagc
     4441 gcagcaaaat gggcggcgtg agaacggcag ccgaaattat caacctgatg aaaacgcagc
     4501 aggaagaagc cgttattacc gccgtgcgtg aattcgacgg cgagctggcg cagaaaatca
     4561 tcgacgagat gttcctgttc gagaatctgg tggatgtcga tgatcgcagc attcagcgtc
     4621 tgttgcagga agtggattcc gaatcgctgc tgatcgcgct gaaaggggcc gagcagccac
     4681 tgcgcgagaa attcctgcgc aatatgtcgc agcgtgccgc cgatattctg cgcgacgatc
     4741 tcgccaagcg tggtccggtg cgtctgtcgc aggtcgaaaa cgaacagaaa gcgattctgc
     4801 tgattgtgcg ccgtcttgcc gaaactggcg agatggtgat tggcagcggc gaggatacct
     4861 atgtctgata atctgccgtg gaaaacctgg acgccggacg atctcgcgcc accaccggca
     4921 gagtttgtgc caatggtcga gtcggaagaa accattattg aagaggccga acccagcctt
     4981 gagcagcaac tggcgcaact gcaaatgcag gcccacgagc agggttatca ggcggggatt
     5041 gccgaaggtc gccagcaagg tcacgaacag ggctatcagg aaggactggc ccaggggctg
     5101 gaacaaggtc tggcagaggc gaaggctcaa caagcgccaa ttcatgcccg gatgcagcaa
     5161 ctggtcagcg aatttcaaac tacccttgat gcgcttgata gtgtgatagc ttcgcgcctg
     5221 atgcagatgg cgctggaggc agcacgtcag gtgatcggtc agacaccaac ggtggataac
     5281 tcggcactga tcaaacagat ccaacagttg ttgcagcaag aaccgttatt cagcgggaaa
     5341 ccacagctgc gcgtacaccc ggatgatctg caacgtgtgg atgatatgct cggcgcaact
     5401 ttaagtttgc atggctggcg cttgcggggc gatcccaccc tccaccctgg cggatgtaaa
     5461 gtctccgccg atgaaggcga tctcgacgcc agtgtcgcca ctcgctggca agaactctgc
     5521 cgtctggcag caccaggagt ggtgtaatga ccacgcgcct gactcgctgg ctaaccacgc
     5581 tggataactt tgaagccaaa atggcgcagt tgcctgcggt acgtcgctac gggcgattaa
     5641 cccgcgctac cgggctggtg ctggaagcca ccggattaca attgccgctc ggcgcaacct
     5701 gtgtcattga gcgccagaac ggcagcgaaa cgcacgaagt agaaagcgaa gtcgttggct
     5761 ttaacggtca acggctgttt ttaatgccgc tggaggaagt ggaaggtgtc ctgcccggcg
     5821 cgcgtgtata tgccaaaaac atttcggcag aagggctgca aagcggcaag cagttgccgc
     5881 tcggtccggc gttattaggt cgtgttctgg acggtagcgg taaaccgctc gatggcctgc
     5941 ccttccccga tacgacggaa accggtgcgc tgattacccc gccatttaac ccgttgcaac
     6001 gtacaccgat tgaacatgtg ctggacaccg gcgtgcgccc aatcaatgcc ctgcttaccg
     6061 tcggacgcgg gcagcgtatg gggctgtttg ccgggtcggg cgttggtaaa agtgtgctgc
     6121 tggggatgat ggcacgctac acccgcgccg atgtcattgt cgtgggtttg attggtgaac
     6181 gtgggcgcga agtaaaagat tttattgaga atatcctcgg tgccgaagga cgtgcacgct
     6241 cagtggtgat tgccgctccg gcggatgttt ctccgctcct gcgaatgcaa ggtgccgcct
     6301 atgccacgcg cattgccgaa gattttcgcg atcgtggtca gcatgtgttg ctgattatgg
     6361 actccctcac ccgctacgcg atggcccagc gtgaaattgc gctggcgatt ggcgaaccac
     6421 ctgccactaa aggttatcca ccgtcggtgt ttgccaaatt accggcactg gtcgagcgtg
     6481 ccggtaatgg cattagcggc ggcggctcga ttaccgcgtt ttataccgtg ctcaccgaag
     6541 gcgatgacca gcaggacccc attgctgact ccgcgcgggc aatcctcgac ggtcacattg
     6601 tgctgtctcg ccgactggcg gaagccgggc actatccggc tatcgatatt gaagcgtcga
     6661 tcagtcgcgc aatgacggcg ttgatcagtg agcaacatta cgcgcgagtg cgcaccttca
     6721 aacagctgtt gtcgagtttt cagcgtaacc gcgatctggt tagcgtcggc gcgtatgcca
     6781 aaggcagcga tccgatgctc gataaagcca tcgccctgtg gccgcagctg gagggctatt
     6841 tgcaacaagg catttttgaa cgcgcggact gggaagcgtc tctccagggg ctggagcgta
     6901 ttttcccgac agtgtcataa cccaggagat aacggcagat ggcggaagaa cagctcaaaa
     6961 tgttgatcga ttatcagaat gaatatcgca ataacctcaa tagcgatatg agtgccggga
     7021 tgaccagcaa ccgctggatc aactatcagc agtttatcca gacgctggaa aaagccatta
     7081 ctcagcatcg ccagcaactt aatcagtgga cgcagaaagt tgacattgcc ctgaacagct
     7141 ggcgagaaaa gaaacaacgt ttgcaggcct ggcagacact gcaggaacgg caatccacgg
     7201 cggcactgct tgcagaaaac cgcctcgatc agaaaaagat ggatgagttc gcccagcgcg
     7261 ccgccatgag gaaacctgaa tgattcgctt agcgcccttg attaccgccg acgttgacac
     7321 caccacattg cctggcggca aagccagcga tgctgcacaa gattttctca cgttgttgag
     7381 cgaagcatta gcaggcgaga caacaactga caaagcggcc ccccagttgc tggtagcaac
     7441 agataagccc acgacaaaag gcgagccgct ggtcagcgat attcttgccg atgcgcaaca
     7501 agcggattta ctgatccctg tggatgaaac acttcctgtc atcaacgacg aacaatccac
     7561 atcaacaccg ttaaccaccg ctcagacgat gacgttggct gcggtggctg acaaaaatac
     7621 gacaaaagac gaaaaagcgg atgatcttaa tgaagacgtc accgcaagtc tgagcgccct
     7681 ttttgcgatg ttgccgggtt ttgacaatac gcccaaagtg actgatgcac cgtcaaccgt
     7741 gttaccggca gagaaaccaa cgctcttcac aaaactgact tctgcgcaac tcactacagc
     7801 acagcctgat gacgcccccg gcacaccagc tcagccatta acaccgctgg tagcagaagc
     7861 ccagagtaaa gcggaagtca tcagcacgcc ttcgccggtg accgctgacg ccagcccgct
     7921 aatcactcca caccagacac agccactgcc caccgtcgcc gcgcctgttt tgagtgcacc
     7981 gctgggttct cacgaatggc aacaatcatt gagccagcat atttcgctgt tcacccgcca
     8041 ggggcaacaa agtgcagagt tgcgtctgca cccacaggat ttaggtgaag tgcaaatttc
     8101 cctcaaagtg gatgataacc aggcgcagat ccagatgatt tcaccgcatc agcacgtacg
     8161 cgccgccctg gaagcagcgc tgccggtact gcgtacgcag ctggccgaaa gtggcattca
     8221 gttagggcaa agcaacatta gtggcgaaag ctttagtggt cagcagcagg ccgcttccca
     8281 gcaacagcaa agccaacgca cagcaaacca tgaacctctg gcgggggaag acgacgatac
     8341 gcttccggtt cccgtctctt tacaagggcg tgtaacaggt aacagcggcg ttgatatttt
     8401 cgcctaacgt cagaggtagc accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag
     8461 acgcaggata attagccgat aagcagtagc gacacaggaa gaccgcaaca catgactgat
     8521 tacgcgataa gcaagaaaag caagcgatcg ctttggatcc cgattctggt attcattacc
     8581 ctcgcggcct gtgccagcgc aggttacagc tactggcatt cgcatcaggt tgccgctgac
     8641 gacaaagcgc agcaacgcgt cgtgccctcc ccggtcttct atgcgctgga tacctttacg
     8701 gtcaatttgg gcgatgcgga tcgcgtgctt tatatcggca taaccctgcg actgaaagat
     8761 gaagcaaccc gctcgcggct gagtgagtat ttgccggaag tccgtagtcg cttgctgtta
     8821 ctgttttcac gtcaggatgc tgccgtactg gcgacagaag aaggcaagaa aaacctgatt
     8881 gccgagatta aaaccacact ttccaccccg cttgttgccg ggcaaccgaa acaggatgtc
     8941 accgacgtgc tgtataccgc ttttattctg cgataacgac atgggcgata gtattctttc
     9001 tcaagctgaa attgatgcgc tgttgaatgg tgacagcgaa gtcaaagacg aaccgacagc
     9061 cagtattagc ggcgaaagtg acattcgtcc gtacgatccg aatacccaac gacgggttgt
     9121 gcgcgaacgt ttgcaggcgc tggaaatcat taatgagcgc tttgcccgcc attttcgtat
     9181 ggggctgttc aacctgctgc gccgtagccc ggacattacc gtcggggcca tccgcattca
     9241 gccgtaccat gaatttgccc gcaacctgcc ggtgccgacc aacctgaacc taatccacct
     9301 gaaaccgcta cgcggcacag ggctggtagt gttctcgccg agtctggtgt ttatcgccgt
     9361 ggataacctg tttggcggcg atggacgctt cccgaccaaa gtggaaggtc gcgagtttac
     9421 ccatactgaa cagcgcgtca tcaaccgtat gttgaagctg gcgctggaag gctatagcga
     9481 cgcctggaaa gcgattaatc cgctggaagt tgagtacgtg cgttcggaaa tgcaggtgga
     9541 atttaccaat atcaccacct cgccgaacga cattgtggtt aacacgccgt tccatgtgga
     9601 gattggcaac ctgaccggcg aatttaatat ctgcctgcca ttcagcatga tcgagccgct
     9661 acgggaattg ttggttaacc cgccgctgga aaactcgcgt aatgaagatc agaactggcg
     9721 cgataacctg gtgcgccagg tgcagcattc acagctggaa ctggtggcca actttgccga
     9781 tatctcgcta cgcctgtcgc agattttaaa actgaaaccc ggcgacgtcc tgccgataga
     9841 aaaacccgat cgcatcatcg cccatgttga cggcgtcccg gttctgacca gtcagtacgg
     9901 tactctcaac ggtcagtatg cgttacggat agaacatttg attaacccga ttttaaattc
     9961 tctgaacgag gaacagccca aatgagtgac atgaataatc cggccgatga caacaacggc
    10021 gcaatggacg atctgtgggc tgaagcgttg agcgagcaaa aatcaaccag cgaaaaaagc
    10081 gctgccgacg cggtgttcca gcaatttggc ggtggtgatg tcagcggaac gttgcaggat
    10141 atcgacctga ttatggatat tccggtcaag ctgaccgtcg agctgggccg tacgcggatg
    10201 accatcaaag agctgttgcg tctgacgcaa gggtcagtcg tggcgctgga cggtctggcg
    10261 ggcgaaccgc tggatattct gatcaacggt tatttaatcg cccagggcga agtggtggtt
    10321 gttgccgata aatatggcgt gcggatcacc gatatcatta ccccgtccga gcggatgcgc
    10381 cgcctgagcc gttagtgatg aataaccatg ctactgtgca atcttccgcg ccggtttctg
    10441 ctgagccact gctgcaggtg agcggcgcac tcatcgccat tattgccctg atcctcgctg
    10501 ctgcctggct ggtaaaacgg ctgggatttg cccctaaacg cactggcgtt aacggtctga
    10561 aaattagcgc cagtgcttcg ctgggcgcgc gtgaacgggt cgtggtggtc gatgtggaag
    10621 atgcacggct ggtgctcggc gttaccgcag ggcaaatcaa tctgctgcat aaacttcccc
    10681 cttcagcacc aacggaagag ataccgcaga ccgattttca gtcggtcatg aaaaatttgc
    10741 ttaagcgtgg cgggagatcc tgatgcgtcg tttgttttct gtcgcaccgg tccttctctg
    10801 gctgattacg cccctcgcct tcgcgcaact gccgggtatc accagccagc cgctacctgg
    10861 cgggtatcac cagccagccg ctacctggcg gtggacaaag ctggtcgctc ccggtgcaga
    10921 cgctggtgtt catcacctcg ttgacgttca ttccagcgat tttactgatg atgaccagtt
    10981 tcacccgcat cattattgtt tttggtttat tgcgtaacgc gctgggaacg ccctccgcgc
    11041 cacctaacca ggtattgctg gggctggcac tgtttttgac cttttttatt atgtcgccag
    11101 tgatcgacaa aatttatgtc gatgcgtacc agccattcag cgaagagaaa atctcaatgc
    11161 aggaggcgct ggaaaaaggg gcgcagccgc tgcgtgagtt tatgctgcgt cagacccgcg
    11221 aggcagattt agggttgttt gccagactgg cgaataccgg cccgttgcag gggcctgaag
    11281 ctgtgccgat gcgcattttg ctcccggcct acgtgaccag cgagttgaaa accgcatttc
    11341 agataggctt cacgattttc atcccttttt tgattatcga cctggtgatt gccagcgtgt
    11401 tgatggcatt ggggatgatg atggttcccc ccgccaccat tgctctgccc tttaaactga
    11461 tgctgtttgt actggtggat ggctggcaat tgctggtcgg ttcgctggcg cagagctttt
    11521 acagctagag aggcaaaatg acacctgaat cggtcatgat gatgggaact gaagcgatga
    11581 aagtcgcgct ggcactggca gccccgctgt tgctggtagc gctggtcacg ggccttatca
    11641 tcagtatttt gcaggcagcc acgcagatta acgaaatgac gctgtcgttt attccgaaaa
    11701 tcatcgccgt atttatcgcc atcattattg ccggaccgtg gatgctcaat ctgttgctgg
    11761 attacgtccg caccttgttc actaacctgc cgtatatcat cgggtagccc gtacaatgat
    11821 gcaggagaca agcgatcaat ggctttcctg gttaagcctg tacttctggc cgttactgcg
    11881 cgtgctggcg ctcatttcta ccgcgccaat tctgagcgaa cgcagcgtgc cgaaacgggt
    11941 aaaactgggt ctggcaatga tgatcacgtt cgccattgcc ccatcattac ctgccaacga
    12001 tgttcctgtt ttttcgttct ttgctctgtg gctggccgta cagcagatcc tgatcggcat
    12061 tgcgcttggt tttaccatgc aatttgcctt tgccgctgtg cgaaccgctg gcgaaattat
    12121 cggtctgcaa atggggctgt catttgcgac atttgtcgat cctggcagcc atctcaatat
    12181 gcccgtttta gcgcgcatca tggatatgct ggcattactg ctgttcctga catttaacgg
    12241 tcatttatgg ttgatttcac tgctggtcga tacctttcac accctgccga ttggtagcga
    12301 accgttgaac agcaatgcgt ttctggcacc caccaaagca gggagtttga ttttccttaa
    12361 cgggctgatg ctggcgttac cgctcattac tctgttgctg accctgaatc tggcattagg
    12421 tttactgaat cgtatggccc cgcaattatc catttttgtt attggatttc cattaactct
    12481 gactgtcggc atctctttaa tggcggcatt aatgccgtta attgcgcctt tttgcgaaca
    12541 tttattcagt gaaattttta atttgctggc tgatattatt agtgaattac cattaatata
    12601 attccgtaac gtttatcatg ctaccctaag gattatccga aaaataatat ctgcgaacat
    12661 cttccaggat actcctgtag cgaaatattt gttttaagct cactcacata tcgcaacatt
    12721 tactttaaga caattccagg caaattatac aacactttac gggatagtaa gtccgcctga
    12781 aaaattgcga gagtggcgca ttaggtgacc catgttgttc cgtttagtca tgatgaaata
    12841 ttcaggtaag gggaattatc gttacgcatt gagtgagggt atgccatgtc aacgattatt
    12901 atggatttat gtagttacac ccgactaggt ttaaccgggt atctgttgag tagaggggtt
    12961 aaaaaaagag aaatcaacga cattgaaacc gttgatgacc ttgccatagc ttgtgattca
    13021 cagcgtcctt cagtggtgtt tattaatgag gactgtttca tccacgatgc ttctaacagt
    13081 cagcgcatca agcacatcat taatcaacat cccaatacgt tatttatcgt ttttatggca
    13141 attgccaatg ttcattttga tgaatatcta ttggtcagaa aaaaatttat tgatcagttc
    13201 taaatcgatt aaaccggaat ctctcgacga tatccttggc gatattctga aaaaagagac
    13261 aacgataacc tcgtttttaa atatgccgac gttatcattg agccgaaccg aatcgagtat
    13321 gttgcgaatg tggatggcag gtcagggaac cattcaaatc tccgaccaaa tgaatatcaa
    13381 agccaagacc gtttcatcgc ataaaggtaa tattaaacgt aagatcaaaa cgcataataa
    13441 acaggttatc taccatgtcg tccgactgac ggataatgtg actaatggta tttttgtcaa
    13501 catgcgctaa cacattctga ctggtggttt cccaccagtc aggctgaata agattactct
    13561 gctttctcaa caaagatacc gtcctgatgc cctgcttcat taaagaacca gatgccgagc
    13621 gggtagtctt ccagcgcaac caggtacatt gtgccttcac taaactcctc aactgccagt
    13681 accactccag gacgacgcgg accgccatcc gttttgactg ttacccgatc attcaccttc
    13741 atcgttttcc tcctgtggct ttgtgccagt gtagaacaat ttcgtttttc tggcagcgcc
    13801 aagcgcgcgc gagtgctgat tttctcgacg gtctatactt aagagatgcc aggcggactt
    13861 aacgactggc ggcaacaaca gagtaacggt tgcgaggaaa gatgatgaaa accgcaaaag
    13921 tgtacagcga taccgcaaaa cgtgaggtca gcgtcgatgt cgatgccctg ctggcggcga
    13981 tcaatgaaat t
//
LOCUS       DAZOYF010000105        13853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZOYF010000105 DAZOYF010000000
VERSION     DAZOYF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.105"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="ACHUOF_002849"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="ACHUOF_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR0611946.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /locus_tag="ACHUOF_002850"
     CDS             321..1952
                     /locus_tag="ACHUOF_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR0611947.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="ACHUOF_002851"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="ACHUOF_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR0611948.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="ACHUOF_002852"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="ACHUOF_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR0611949.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="ACHUOF_002853"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="ACHUOF_002853"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0611950.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="ACHUOF_002854"
     CDS             6426..7055
                     /locus_tag="ACHUOF_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR0611951.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="ACHUOF_002855"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="ACHUOF_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR0611952.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="ACHUOF_002856"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="ACHUOF_002856"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR0611953.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="ACHUOF_002857"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="ACHUOF_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR0611954.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11463..13730
                     /gene="adiA"
                     /locus_tag="ACHUOF_002858"
     CDS             11463..13730
                     /gene="adiA"
                     /locus_tag="ACHUOF_002858"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR0611955.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3615 a         3588 c         3362 g         3288 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaagctg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaacaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac tggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgatg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa
    11461 tgatgaaagt attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg
    11521 ccgttgagcg tctggcagat gctttaagcc agcaaaatgt taccgtgatt aaatccacct
    11581 cctttgatga tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca
    11641 gctatcaaat ggagcatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc
    11701 atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg
    11761 caatggaccg ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg
    11821 ccgactttat cgccggacgc gccgttgccg cgatgacccg ctaccgccag cagctgttgc
    11881 cgccactgtt cagcgcgctg atgaaatata gtgacatcca tgaatattcc tgggcagcgc
    11941 caggccacca gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact
    12001 actatggtga aaatctgttc cgtaccgaca tgggcatcga acgaacttcc ctcggttctt
    12061 tgcttgacca tactggcgca tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg
    12121 ccgatcgctc ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg
    12181 cttgcatgac cgataacgat gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac
    12241 aaggtttgat gctgacaggc gcgaaaccgg tctatatggt gccaagccgc aaccgctacg
    12301 gcattatcgg gccaatctat ccgcaggaaa tgcaacctga aaccttgcaa aagaaaatca
    12361 gtgaaagccc gctgaccaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtaacca
    12421 actgcaccta tgacggcgtg tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa
    12481 cctccgatcg tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct
    12541 atgccgatca ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg
    12601 ccacccactc catccacaaa ctgctgaatg cgctgtcaca ggcttcttat attcatgtac
    12661 gtgaaggtcg tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca
    12721 ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg
    12781 gcaacagcgg cctgtcactg acacaggaag tgattgacga agcggttgat ttccgtcagg
    12841 cgatggcgcg gctatataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga
    12901 acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa
    12961 ccaaactgct gaccaccgtt caggactgct gggtaatgca tccgggcgaa agctggcacg
    13021 gcttcaaaga tattccggat aactggagta tgctcgaccc aattaaagtc agcatccttg
    13081 ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca
    13141 ctgcctggct tggtcgccac ggcattgtgc ctacccgcac cactgacttc caaattatgt
    13201 tcctgttctc tatgggcgta acccgtggga aatgggggac tctggttaac accctttgct
    13261 ccttcaaaca ccactatgac gccaacacac cgctggcgca ggtgatgccg gaacttgttg
    13321 aacaatatcc tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg
    13381 cctggctgaa agaaaacaac cctggcgcac ggttgaacga agcctattcc ggcctgccgg
    13441 tggcggaaat caccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg
    13501 tatccattga aaatctgcca ggacgcatcg cggcaaactc agttatcccg tatccgccag
    13561 gaatcccgat gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt
    13621 atttacgctc gctgcaatcc tgggaccacc atttccctag atttgaacac gaaactgaag
    13681 ggactgaaat tattgacggt atttaccacg ttatgtgcgt gaaagcgtaa ccactattcc
    13741 gctgaaggcg taattgttta aataacatta cgccgcctgg ccttaggcct tttgagtatg
    13801 gcaacgtttt cataaaaatt gctgcaaaca aaaatgtcat actttttgcg cgg
//
LOCUS       DAZOYF010000106        13462 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZOYF010000106 DAZOYF010000000
VERSION     DAZOYF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.106"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            565..1215
                     /gene="csgD"
                     /locus_tag="ACHUOF_002859"
     CDS             565..1215
                     /gene="csgD"
                     /locus_tag="ACHUOF_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR0611956.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            1220..1609
                     /gene="csgE"
                     /locus_tag="ACHUOF_002860"
     CDS             1220..1609
                     /gene="csgE"
                     /locus_tag="ACHUOF_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR0611957.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1634..2050
                     /gene="csgF"
                     /locus_tag="ACHUOF_002861"
     CDS             1634..2050
                     /gene="csgF"
                     /locus_tag="ACHUOF_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR0611958.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2077..2852
                     /locus_tag="ACHUOF_002862"
                     /pseudo
     CDS             2077..2852
                     /locus_tag="ACHUOF_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
     gene            complement(2916..3407)
                     /locus_tag="ACHUOF_002863"
     CDS             complement(2916..3407)
                     /locus_tag="ACHUOF_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR0611959.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3509..4063)
                     /gene="ycdY"
                     /locus_tag="ACHUOF_002864"
     CDS             complement(3509..4063)
                     /gene="ycdY"
                     /locus_tag="ACHUOF_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR0611960.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4087..4824)
                     /gene="ycdX"
                     /locus_tag="ACHUOF_002865"
     CDS             complement(4087..4824)
                     /gene="ycdX"
                     /locus_tag="ACHUOF_002865"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR0611961.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPCRLLNFLESRGMAPIAEFADL"
     gene            complement(4879..5817)
                     /gene="ghrA"
                     /locus_tag="ACHUOF_002866"
                     /pseudo
     CDS             complement(4879..5817)
                     /gene="ghrA"
                     /locus_tag="ACHUOF_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6052..6139
                     /locus_tag="ACHUOF_002867"
     tRNA            6052..6139
                     /locus_tag="ACHUOF_002867"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6086..6088,aa:Ser,seq:gga)
     gene            complement(6289..6570)
                     /locus_tag="ACHUOF_002868"
                     /pseudo
     CDS             complement(6289..6570)
                     /locus_tag="ACHUOF_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(6852..7856)
                     /locus_tag="ACHUOF_002869"
     CDS             complement(6852..7856)
                     /locus_tag="ACHUOF_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HFR0611962.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(7928..8488)
                     /locus_tag="ACHUOF_002870"
                     /pseudo
     CDS             complement(7928..8488)
                     /locus_tag="ACHUOF_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(8573..8770)
                     /locus_tag="ACHUOF_002871"
     CDS             complement(8573..8770)
                     /locus_tag="ACHUOF_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFR0611963.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(8782..9273)
                     /locus_tag="ACHUOF_002872"
     CDS             complement(8782..9273)
                     /locus_tag="ACHUOF_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFR0611964.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(9270..9644)
                     /locus_tag="ACHUOF_002873"
     CDS             complement(9270..9644)
                     /locus_tag="ACHUOF_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFR0611965.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(9691..10065)
                     /locus_tag="ACHUOF_002874"
     CDS             complement(9691..10065)
                     /locus_tag="ACHUOF_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFR0611966.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(10228..10449)
                     /locus_tag="ACHUOF_002875"
     CDS             complement(10228..10449)
                     /locus_tag="ACHUOF_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFR0611967.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(10512..10988)
                     /locus_tag="ACHUOF_002876"
     CDS             complement(10512..10988)
                     /locus_tag="ACHUOF_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFR0611968.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11004..11477)
                     /locus_tag="ACHUOF_002877"
     CDS             complement(11004..11477)
                     /locus_tag="ACHUOF_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR0611969.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(11819..12640)
                     /locus_tag="ACHUOF_002878"
     CDS             complement(11819..12640)
                     /locus_tag="ACHUOF_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR0611970.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(12740..12913)
                     /locus_tag="ACHUOF_002879"
     CDS             complement(12740..12913)
                     /locus_tag="ACHUOF_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFR0611971.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13033..>13462)
                     /locus_tag="ACHUOF_002880"
     CDS             complement(13033..>13462)
                     /locus_tag="ACHUOF_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0611972.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3432 a         3302 c         3269 g         3459 t
ORIGIN      
        1 acgctgttat ctacgcaaaa aatattttgt tttctcttaa atctccgttt tccgctatca
       61 aaaatcacca gacagtcatt cttcttgccc gtcgctgatt gctgcgatat gtcttgcgca
      121 caagccgtga caaacctcgt ctttttgcag taaaaaaatt gtccacggag gtgtggagaa
      181 aaaacaaaaa cgttttacat gacgaaagga ctacaccgaa atatttttta tatgtattat
      241 tagtaagtta gcaccatttg tatgattttt taaaattgtg caataaaaac caaatgtaca
      301 acttttctat catttctaaa cttaataaaa ccttaatgtt aacattttaa tataatgagt
      361 tacattttgt tacatgttta acacttgatt taagatttgt aatggctaga ttgaaatcag
      421 atgtaatcca ttagttttat attttaccca tttagggctg atttattact acacacagca
      481 gtgcaacatc tgtcagtact tctggtgctt ctattttaga ggcagctgtc aggtgtgcga
      541 tcaataaaaa agtggggttt catcatgttt aatgaagtcc atagtattca tggtcataca
      601 ttattgttga tcactaaacc ttctttgcag gcgacagctc tcttgcagca ccttaaacaa
      661 tcgctagcaa ttacaggaaa attacataat attcaacgtt ctctggacga tatctcttca
      721 ggctctatta ttcttctgga tatgatggaa gcggataaaa agcttatcca ttattggcag
      781 gatactttga gcaggaaaaa caacaatatc aaaatattgt tgctaaatac gcctgaagat
      841 tacccgtacc gcgacattga aaactggcct catatcaacg gcgtttttta tgccatggag
      901 gatcaagaac gtgttgtcaa tgggttgcaa ggcgtcctgc gtggcgaatg ctactttacg
      961 caaaagcttg ccagttacct gattacacat tctggtaact atcgttataa cagcacggaa
     1021 tcagccctcc ttactcatcg ggaaaaagag atcctgaata agctgcgtat cggcgcgtct
     1081 aataacgaga tcgctcgttc gttgttcatc agcgaaaata cggttaaaac gcatctttat
     1141 aatcttttca agaagatagc cgtaaaaaac cggacacaag cggtttcctg ggcaaacgat
     1201 aacctcaggc gataaagcca tgaaacgtta tttacgctgg attgtggcgg cagaatttct
     1261 gttcgccgca gggaatcttc acgccgttga ggtagaagtc ccgggattgt taactgacca
     1321 tactgtttca tctattggcc atgattttta ccgagccttt agtgataaat gggaaagtga
     1381 ctatacgggt aacttaacga ttaatgaaag gcccagtgca cgatggggaa gctggatcac
     1441 aataacggtc aatcaggatg ttattttcca gactttttta ttcccgttga aaagagactc
     1501 cgagaaaact gtcgtctttg cactgattca aactgaagaa gcactaaatc gtcgccagat
     1561 aaatcaggcg ttattaagta cggacgattt ggcgcatgat gaattctaaa taaaaaattg
     1621 ttcggaggct gcaatgcgtg tcaaacatgc agtagttcta ctcatgctta tttcgccatt
     1681 aagttgggct ggaaccatga ctttccagtt ccgtaatcca aactttggtg gtaacccaaa
     1741 taatggcgct tttttattaa atagcgctca ggcccaaaac tcttataaag atccgagcta
     1801 taacgatgac tttggtattg aaacaccctc agcgttagat aactttactc aggccatcca
     1861 gtcacaaatt ttaggtgggc tactgtcgaa tattaatacc ggtaaaccgg gccgcatggt
     1921 gaccaacgat tatattgttg atattgctaa ccgcgatggt caattgcagt tgaacgtgac
     1981 agatcgtaaa accggacaaa cctcgaccat ccaggtttcg ggtttacaaa ataactcaac
     2041 cgatttttaa gccccagctt cataaggaaa ataatcatgc agcgcttatt tcttttggtt
     2101 gccgtcatgt tactgagcgg atgcttaacc gccccgccta aagaagccgc cagaccgaca
     2161 ttaatgcctc gtgctcagag ctacaaagat ttgacccatc tgccagcgcc gacgggtaag
     2221 atctttgttt cggtatacaa cattcaggac gaaaccgggc aatttaaacc ctacccggca
     2281 agtaacttct ccactgctgt tccgcaaagc gccagggctt acaaaacctg cttaacgagc
     2341 gcaaggttat tcgtgcggca caagaaaacg gcacggttgc cattaataac cgaatcccgc
     2401 tgcaatcttt aacggcggca aatattatgg ttgaaggttc gattatcggt tatgaaagca
     2461 acgttaaatc gggcggtgtt ggggcaagat attttgggat cggtgcagac acgcaatacc
     2521 agctcgatca gattgccgtg aacctgcgtg tcgtcaatgt gagtaccggt gagatcctct
     2581 cctcggtgaa caccagtaag acgatacttt cctatgaagt tcaggccggg gttttccgct
     2641 ttattgacta ccagcgcttg ctggaagggg aagtgggtta cacctcaaac gaacccgtta
     2701 tgctgtgtct gatgtcggct atcgaaacag gggtcatttt cctgattaat gatggtatcg
     2761 accgtggtct gtgggatttg taaaataaag cagaacggca gaatgacatt ctggtgaaat
     2821 accgccatat gtcggttcca ccggaatcct gacagataaa aatgccggaa ttaataatcc
     2881 ggcatttact gtttaagcga aagcgtgagt gcccctcacg ctttattttt gatttcctgc
     2941 tcacggtgag ccgtctttgc actacgcgcc gccagccaca ggccgctgtt tttcattgcg
     3001 taaccaaata ccagcccaag cgccagcgaa ggtaacacca gtttccagtc accctgcccg
     3061 gcaaacgtcg cgcatgcgcc tataaacgtc cccggaacaa atgaaagcag cagctgtttg
     3121 gcctgaatac acatcagaaa cgcgacaata ccggttatga cataaccgag aatttccaga
     3181 tgtggtgcca gtgcactacc gtaaataatg accatcgccc acaccacgcc actaagcagg
     3241 gttgcagcgg agatcgccag ccctttcagc ccaccttgcg ggcaagcaaa gtaggccgta
     3301 cagcccagga agcccgccca gcttagtaag cccagggaaa cagccaccca tccccagata
     3361 ccggagagaa tgcctgttgt gattgcaata gagagaagta tgttcatgcc gcgcatgata
     3421 gcagaaaacg cggccatgaa tgagatcgag cacacatttt atgcaacaac aaattatctg
     3481 ttgcagttat gagtgacgat gatcacattt actcttcaga atcttcttcc agctcgtccc
     3541 acattgcact aatggcatca cgggttagcg gtgccatggt gcgccagaaa ggcgtggttg
     3601 catgggcctc cactttgccg aggaacgcac cacaccaggg taacagatac tcactgaaca
     3661 gtgtttccag tgcttcgctc tcatcttccg ttgactgatc ttccagccag gaagccgcaa
     3721 gcagcaatgt gccgatgtga tcggctggcg tatccgccag tagcatccct cgttcggaaa
     3781 gaaaagcgcg cacttccgct tccgttgcgt cctcaaccca tgcgctacga tatggcggca
     3841 cggcacattc atcgccgata aacaacgcat tgtaatcggc agagacttgc gtcatatcac
     3901 agcttttctg taaacgcgtc agtaactcat cctgctccag tggccagttc gcagccagtt
     3961 tcccctcacg aatcagagta aacagcggca ccagtaaagg atcttgcggt tggcggtaat
     4021 agagcgaacc cagcacacga cagaggatag aaaactcgtt cattaatata tttccattac
     4081 tagtaattaa aggtctgcaa attccgcaat cggtgccata ccgcgagatt caaggaagtt
     4141 cagtaagcgg cacggagaaa cattcaaaat gcgctctagc ggaaaatcta ccgcgtcgag
     4201 gattttaaga cactcttcaa attcccccat ggtaaacgcg gtgtgagaat ccgagcctaa
     4261 tgccacccaa cctccagcat cacgtaccgc tgcagctacc gcacgacagt tgtcttcact
     4321 gcccttacgt gagtgtaaaa atgaggaatt attgatttcc agcgccacct gatgtttcgc
     4381 ggctgcttca gcaacggctt taacatctat ttcatatttc ggatttccgg gatggcttat
     4441 tatatgcaca ttgccgctgg cgatagtgga gatcatcgct tgtgtattgg tcgctttgtc
     4501 atgtggcgca aaaaccggct catgaaaacc ggcaataatc agatccagcg agtcaaacat
     4561 tttaccgctg cagtcaattt caccatcaac atttttaatg ttagcttcaa tgccgcgcag
     4621 gatccctacc ccatcaacca ctcgcggcca gatacgcatg ttaatgaagt gccagtgatg
     4681 cggcgcatct tccatatccg ggccatgatc ggtgatcgca aaaagtttaa tgcccttttg
     4741 tttggcctgg gcaatgtaat cactcaatgt gctatatgca tgtgtgctgg caacggtatg
     4801 catatgaagg tcgacgggat acataactct ctcctgtatt caattcccgc caaggatagc
     4861 aggaatcctg atgctttatt agtagccacg tgcgcggtcg acttgcccgc agaccctctc
     4921 ccctttttcg agctgggcaa tagtgcgaga aatgtactcc acagcttcag cgggacgggt
     4981 aatcgcggcg acatgtggtg ttatcgtcac gcgtggatgt tgccagagcg gactttcagg
     5041 cggtaagggt tcacgattaa aaacatccag cattgcgcct ttaactttcc cgctatccag
     5101 cgccgcgagc aggtcatctt ccacaacatg aacaccacgc gccaggttga ggagatacgc
     5161 gccatcaggt aatttttcga gtaattgttg attaataatg ccgacggttt caggggtatt
     5221 cggtaacaaa ttaatcaata cccgacattg gctcagaaat gcagacagtt cttcccgtcc
     5281 ggcaaagctt tgcacgccag gccacgattt acgggttcga ctccagcaac gcagcggaaa
     5341 gcgctaggtt tgcagactct gagcaacttt acagcccagt acgcctgcgc ccaaaatgcc
     5401 gatggtaaaa tcttcccgat gatattcagg cagcggttgc caatgcgaac tattttgctg
     5461 gatgcgataa tcgtcaaaac gtcgaaacca atgcagcacc tgactgacag catattcctg
     5521 catttgctcg cccataccgg tatcttccag gcgaaaaagt ggaacagaag ggttcagcat
     5581 ttcagggtgt gcctgtagct tgctcaaaat agaatcaaca ccggccccga gtgcgaacac
     5641 cgctttaaga tcgcgccctg ccagcatttc aacaggagga tgccagacta aaacataatc
     5701 agcagagtca ttatctccgc ttttccatgc cctgactctt gcctgaggaa tagctttgcg
     5761 cagtgcctca atccaccatt gggtatcgaa cgttgggtga taaaagatga tatccatact
     5821 gactcccgaa aagcgttgtg cgaaatttat tcgcacttat cgttatgatc tacaaaggcc
     5881 accagcataa caaatccgtg gtcggtggca aaaaaagcag atttcgctta ttaaaaccac
     5941 acattgattg aatgtttgaa taaacgcgcg attttttcaa aaagtttgtt gacctcaggt
     6001 catgatttcc ctaaattagc gcccgttcca gcaagacagg aacgacaatt tggtgaggtg
     6061 tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat cgggggttcg
     6121 aatccccccc tcaccgccag attataagtg catgttattg tttaacatgc gcttttttat
     6181 ttttccttcc agacagtatg ccatcgggta tgccatctga aaccggttta tatgccccgc
     6241 gatcatatct tatcggacaa tttccgatct ttgagcatac tggcaaagct atactgccag
     6301 catatcagga tagtgttttg cgataatatc attcagtctg tcaaccagtt ctggttttct
     6361 gaacgtgata tgcgcggagc ccttcttaaa gtatctgatg ctgaacatct catcttcata
     6421 gctctcttta ccctgaacag actgaatgtg atcatccaga cgaagggtga tgtttccccg
     6481 gttgtcgggg attggtttac cgctgaacag gtgcaacatt ctttccagat cggcgagtcg
     6541 gtcagcctgt tgtccggtga taagatgaaa tctatggtca cccccgtttt tgcaacactg
     6601 atttttgatt aatggttggc ttgcttaaat ctatccggcg tctctgtggg caaggatgcc
     6661 cgcgccccga tgagttccgc acctgataat cctaaaaatc ccctcggctt cagtgagcca
     6721 tttcatctgt taggttttta atcaggtcga tttgccgttc atgtcatcaa taatcagtct
     6781 tcgcaaaacc agaaaggtaa cttgtatctt attgtttaaa ataaactaat tgagttatat
     6841 cagcatctat atcaggcctg atattctttt ttctgagtag tgattgccca ggcaattcga
     6901 gccagtttat ttgccagagc gcaggtaacc acattcgaat gcttcttgtt gagttgctcc
     6961 cgaacccact cagcaagttt tccatgcttg cgttcaagtc gcattatgaa tgagcgggca
     7021 cactgaacca gtaaccggcg taaattttta tcacctcgct tactgatacc caacagagtc
     7081 gattttcctc cagtgctata ctgccgggga acaagtcctg tggaagctgc aaaatccctg
     7141 ctgcatgaga actgtttccc gtcaccaagt tgcgacgata gtaagctggc agtaatcgga
     7201 ccaacaccag gaattgtcat aatgcgctga gcggtatcat cagcgttgat tgactgactt
     7261 aattctgatt ccagctctgc tatttgctca acaaggtaaa agtagtgggt atgcaacctc
     7321 accaaaaggc ggctcagata ttcggggatt tcgtgttcag ccaacacgag agacaaccgt
     7381 tttattacag catcacctat cggcaggctg attccaaatt ccagcaagaa accgtggatc
     7441 tggttggttg ttttcacctt atctctgatg agcgattctc tgacccggtg taacgccctc
     7501 atggcctgct gggtctcagt tcttggctgg acaaaacgca ttgatggtcg tgaagccgct
     7561 tcgcatatgg cttctgcatc aacaaaatca ttcttattac ttttgacaaa cgggcgaacg
     7621 aactgtggag aaatcagctt agtttcatgc ccaaaatcag agatacagcg agccataaag
     7681 tgtgcacccg cacaggcttc cattacaaca actgagggcg gacatgtcgc cagaaactgc
     7741 atcagtttag tacgggtaaa ttttttacgc agaagagcct taccagactt gtcttgacaa
     7801 tgaatgtgga aggagtgctt tccgagatcg ataccaataa gtgtgatgtt ctgcatgatg
     7861 gtctctccgt taaagggatt taccttaaca gcgtactgct gttaaggtgg gggtgaccat
     7921 atcattatcc ccaccggtcc catctcacca gattgttgac gataatttta ctgccaaatt
     7981 tacaggggca attggttttg taattccagt tcagccctct gaagacgttg atcacccctc
     8041 gctcaaacac ctcatcctta ttctggtgca gttgttcaaa tgtgctcaga atgttcgctt
     8101 cgctgatttc cggaaaatta tcgtactcca gtgaccggta ccatgtatca cgggcctgcg
     8161 tatccattaa tgacagcatc ccggaacgtt gcatcaggtc acgccagatt tcgcgatcaa
     8221 gattccgggt aatggctttc attgctgttt cgggtttctc catcagccag cagccgcagc
     8281 gaaaatcctg tttcatggcc cagtccgggg cagttttacc tccgatgctt tccgtcagca
     8341 ccgagatttc agccagttgt cggataagtg tgtcgatttg caacagtgta ctctttcgta
     8401 cagccagaat gtggcgaatg ctggttgagc agataacgtc cgtatgcccg ttgaccactt
     8461 ccggacggga gattgtgata tctgacataa tcgatattcc tttttgcaga taaatgaaaa
     8521 cgccagccct gtgcaggctg gcgtcagagg aatgaagtct ggtgagtgag cttcaccggt
     8581 tctggtgcag aagctgcaga tgacgcaggg tacggacatc ctcccgggcc tgttcgatac
     8641 agggcaacag tgctgctgaa tccgttttat cctcatcgtt gtcaaagata attcccgatc
     8701 cgcagtcgat attgtcctgc agccacgcaa tcagaatatc cagcgctgtt tccgtggtta
     8761 atgatttcat gttgttcctg gttaccagtc gaaagtgggt aaacccggca ggcattcagc
     8821 gctggcatcc agatgaatga gactgacgcc ataacgccgg atgagtgtga cgaccagacg
     8881 acggaacgtt tttgacagcc ccaggcgttt aagccgcaga accgggtatg accacgcatc
     8941 ggtacgtaac agatatccgc tacctgtaaa atgaatccat tctgattcac cgaagtcact
     9001 ggtctggtgt gacagcgagt acaaccaggc gttgtccgtt tccgtgatat gtgcggtact
     9061 gcagcgtatg ccggtaaggg tagcaaatgg tggcgtgcat tctgctgagg tctggtcaga
     9121 ttcatcaaca acacgaaggg agtagccgtt atcgcagacc atgttaatca gttcagcgag
     9181 ctcaatacca tcgacgtcaa cgacaatgcg ccctatgttc agtgcctgta cgttaacgct
     9241 gtcggcttcc agtgtcaggg ccagtttcat catttcgcct ccggatactt acccagggta
     9301 atgttattta ccgttctgta attgtcgcgg gtcatcaggc cggtcgcccg gcgagcccgg
     9361 aggatatcaa tactgtttat taactgagag cggggacagg tgctgaatcc cggctggtcg
     9421 gtacgcacca gcgcgtattt ttccacgaga aaattaactg catcacacag tgaaatgcct
     9481 gcctcaatat gctgctcaat cacacgttca tcgacaaacg gtgtgtcatt cagtgtgagg
     9541 ccataatgct ggtccagcag tcggatgagc agtgtctgcc agatggtgac gggagacggg
     9601 cagcgagata cctcccgtac atgtgtgtcg gataatgttt tcatcttaag ttctctgtaa
     9661 ctggtagtgc taaaggtgag agcttcgggg ttatgaggtg gttgcgggtg ctggtgtcgg
     9721 ataaacagcc agataaacgt aaccacagga gccgagggtg tcggcttcgc aggtcagccc
     9781 ttttgcatac agcgtgacgg tatgctgata tcggggattc agttcaccgc tggtgagcat
     9841 gagttccagt tgtttcatca gcagcggaaa ggcctggtcc agatggtatg catctgcgtc
     9901 gctgaagcgg cctctgatac cggcgcggtc ggcaaggtaa tacaggcggt taccctcttg
     9961 caccagacga gcgccaaaac agggcctcac tgtgcaggga agcccccacc aggggcggtc
    10021 gtgattgtca tcgggatgag ttatcccgga gagtttgtct gacacgataa aatcctctct
    10081 gaaaagtggt taagaatggt ttaacgatga caggtcagcc ggcgcgctga ccggtttcgg
    10141 tgaagatgta tgcattgatg agaatggcct catccacagc ggcgtctgag gtcatctggt
    10201 cgtactcgtt tcccgggtga cggaacatca gttcagggtg acactcatca gccggacata
    10261 cgggccaaaa ctgtccttac ggcgttctgc aaacacggca agaacgccgg gaatatcctg
    10321 cacttcacga ccagtatacg cttcagcact gccgtgccag cgatacttac cggtacagaa
    10381 cggaaaaaga cgggatgcag gatgctgttg gtgaatacgc atggcttcac cacgggtgat
    10441 gattctcatg atgtgatacc tctgaagaca gaagataaaa gtgaaaacag gtgtgatgtg
    10501 gttgtgacgg gttaaagcag accatgttcg gcaaaggaga aaacctggtt gccaccgact
    10561 atcagatggt ccggcacccg gatatccacc aggcccagtg cctgtaccag acgttcggtg
    10621 ataagccggt ctgccttact gggtgtgact tcaccggacg ggtgattgtg cgccagcacc
    10681 acggcagcgg cattgtggta cagggcgcgt ttaatcactt cccggggatg aacttccgta
    10741 cggttgatgg tgccggtgaa gagggtttca ccggcaatca gctgattctg gttgttcaga
    10801 tacagcaccc ggaactcttc ccgctccagt cccgccatgt tcagaatcag ccattcccgt
    10861 gccgcacggg tggaggtgaa ggccacgccg ggttcatgaa gatggcggtc cagggtttgc
    10921 agggcccgca gaatgagact gcgctcgccg ggcgtcatgt ttccgggcag aaaggaaagc
    10981 tgctgcattg tccctgcctc cattcagtcg atgatgcgca taatggcgct gcattccgga
    11041 tgctgcaggg cgtaatcccg cagccggtaa taatggaccg tcatggcata acactccgta
    11101 cgacaggcat gatgactgta cgtcatcaga caggcagcaa tgccggcggc ttccgggctc
    11161 atttcagcgc ggttaccgtt catggcattg aacagtaccc atgtttcgtc atcatcgtca
    11221 tccggttcgg gtgccataaa tgccccgccg ttgttcaggg tgtacagatt ccagatacca
    11281 ccgcagtagt cttcacacag acggtccatc cagccgaaga cacggggttc cagggtgacc
    11341 cactgtggaa tgaggccaaa atgctgcggc cagaagctga tgcgctgttc atcagggacg
    11401 ggagtggcaa ccagttgttg caggccagtc tccggtgcac aggccgaagc ggtggtgttc
    11461 tgagacaatg ttttcatgta tgaaatcctt gtaatgtgaa taaatgagag ggagtaaagg
    11521 tcgggagctg gcaacatcac gttcatcact gaccgtgatg acccggtaat atcagggatg
    11581 aatgatattc ggctgaaatt ccggacagcc gtacgcgtaa ccgttccggt cacagacgga
    11641 tggccagatg ccattgcctg tatactcagt ctgcacagac tgatttctcc gggacggtaa
    11701 agcagacggg tacaccaggc gcacaggtca tcatcagaaa tggaacgaca ccccggcagc
    11761 gcctccgggg cgtcgggtga ttctggtgcc tgaatcacgc cttcagggac atgctgcctc
    11821 agcgcaggct ctccagcagc gtttctgcca ttacccacaa tgcgcggttg agcttaatat
    11881 cggtgtcgat gttgtgaatg gcacgggtgt ggatacgttt tccttttgca ctgcggccgg
    11941 aaatcccgcc tttcagcata ttctcctgga tggtctgata tgcgctccac aggtctttac
    12001 cgtaatcctc cctgcgtcgt ggtgtcagaa tgtcagcggt ggtgactggc tgatgttcgt
    12061 caccataacg gtaagtcagt gccgcctgtg ccagcgcctg gcgtgccggt ggcggcagga
    12121 ccagcgactg catggcatca cgcttctcct ctatccggtc aaacacgccc accacctcgt
    12181 aagccccttc aataactttc tccaccacat ttccccggtg tggaacacgc aattctccca
    12241 gagactgacc gcagaccagt ccattggtac aaatggcacg aaaatatccg ggtaacatct
    12301 ggtagctgga tgaaccgtca tgagagttga gcagaataat ttcagggaca tgctgaccgg
    12361 ttatctgtcc ggcccgccgc agacgcagca tgtgttttgt gtattcccgg cgacccgggt
    12421 cacgcacacg tgtctggcag gcgaagaatg gctgaaagcc ttcccgctgc aggctttcca
    12481 gtacggtgat ggtgggagtg tacgcatagc gtttactgcg ggaagtatgc cggtcttcac
    12541 cgaaaatact gggaacatag tgcatcagtt cttcgtgtgt cagcggacgg tcacggcgta
    12601 tctggtttgc agcataacca aaacgactgg ctagtcgcat aatttgctcc ttatcggtgg
    12661 ttaagattta ctggtgtaat aaatgaaaaa gccacgtctc ccggagaaga cgcggcctga
    12721 tggacgaaat taatgaggtt tattgtctga gaagaccgtc actggcgaac cttccggcgt
    12781 cagtctctgc gttgcggata acccgacgga aatgagttcc ctggtcatcg tcgataaaca
    12841 cattgcggta agctgttgtg atgacaacag cctgctcgcg tgagaaaggg acttcgggca
    12901 ataaacgtga catacaacca gacctgtatt tggttccctg aaaatataag tccctgtcat
    12961 catggcagag ccgtatttac ttttgatatc aataaattaa taacgcgaag atgaaccgca
    13021 taacttacgc ctttaatttg ttttgtttac gtagtccctg accaggtacg cgaattcgtg
    13081 aacgccagtt tcaccaaacc aaatcaggta accagggtcg atgttgtaat agtaaccaac
    13141 gactttatgc gtttttgggt tatagagtgt tacgatatag tcccagccgc aggcgtgggc
    13201 tttacaaccg ccaccttcta cccaggtcgt tccgttaatg tttacaggat acgcaccacc
    13261 atcaggacca tccatatagc cgtcttcccc aaacattccg tttaacccaa cagggtttaa
    13321 tgctttgtcg atagcagctc tgtaggtagc gtcaccttcg tataactcgt acgttgttgg
    13381 acatgcacca ggctggtcag cccgggcttt tgagtcgcat ttaagtgtct gatttacagt
    13441 ggcggcgttt gtgtcaggac tt
//
LOCUS       DAZOYF010000107        13125 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZOYF010000107 DAZOYF010000000
VERSION     DAZOYF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.107"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(109..429)
                     /locus_tag="ACHUOF_002881"
                     /pseudo
     CDS             complement(109..429)
                     /locus_tag="ACHUOF_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            complement(635..1354)
                     /locus_tag="ACHUOF_002882"
                     /pseudo
     CDS             complement(635..1354)
                     /locus_tag="ACHUOF_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1370..1702)
                     /gene="cusF"
                     /locus_tag="ACHUOF_002883"
     CDS             complement(1370..1702)
                     /gene="cusF"
                     /locus_tag="ACHUOF_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR0611973.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1726..3108)
                     /gene="cusC"
                     /locus_tag="ACHUOF_002884"
     CDS             complement(1726..3108)
                     /gene="cusC"
                     /locus_tag="ACHUOF_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR0611974.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3265..3948
                     /gene="cusR"
                     /locus_tag="ACHUOF_002885"
     CDS             3265..3948
                     /gene="cusR"
                     /locus_tag="ACHUOF_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR0611975.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3938..5380
                     /gene="cusS"
                     /locus_tag="ACHUOF_002886"
     CDS             3938..5380
                     /gene="cusS"
                     /locus_tag="ACHUOF_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR0611976.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5530..7767
                     /gene="nrfB"
                     /locus_tag="ACHUOF_002887"
     CDS             5530..7767
                     /gene="nrfB"
                     /locus_tag="ACHUOF_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFR0611977.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7754..10722
                     /gene="nfrA"
                     /locus_tag="ACHUOF_002888"
                     /pseudo
     CDS             7754..10722
                     /gene="nfrA"
                     /locus_tag="ACHUOF_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10723..11613
                     /locus_tag="ACHUOF_002889"
     CDS             10723..11613
                     /locus_tag="ACHUOF_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR0611978.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11796..12557
                     /gene="envY"
                     /locus_tag="ACHUOF_002890"
     CDS             11796..12557
                     /gene="envY"
                     /locus_tag="ACHUOF_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR0611979.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12603..12679)
                     /locus_tag="ACHUOF_002891"
     tRNA            complement(12603..12679)
                     /locus_tag="ACHUOF_002891"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12643..12645),aa:Arg,seq:tct)
     gene            12922..>13125
                     /locus_tag="ACHUOF_002892"
     CDS             12922..>13125
                     /locus_tag="ACHUOF_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFR0611980.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3219 a         3405 c         3330 g         3171 t
ORIGIN      
        1 atccctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca
       61 gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcagt tgtcgaacca
      121 gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa aataagtgcg
      181 tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc tggcatcctt
      241 caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat gccagcgata
      301 gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc catcagcatc
      361 agccaattct tgtgggccag aatatggttt accataaata gtacgcagtt ttgtgtcgct
      421 tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac atctggtaca
      481 caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg tagacatact
      541 caggtgaaaa aaaggttgtt tttcgagtta taacagacag ttaattaact acagtggaca
      601 atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg accacgttat
      661 ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca tcaatgggcg
      721 ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga atatccgcct
      781 ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc gccgtaccgc
      841 cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga atggtcaggt
      901 cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac accttgtcga
      961 taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa ctgacattcg
     1021 tcgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt ttcaccccca
     1081 gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct tcatcggcat
     1141 atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg aaccgcgtat
     1201 tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc attttttctg
     1261 cgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata ccgcccgcga
     1321 tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt tactggctga
     1381 ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac gccactttgt
     1441 cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc atggtcatct
     1501 ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg ctttccagat
     1561 caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg ctcatggttt
     1621 catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga ctgaacattg
     1681 cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt gctgccaacc
     1741 gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat tcaggtcaag
     1801 taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca gataacttac
     1861 cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg acgccagata
     1921 acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg catctgccac
     1981 ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct gctggcgaat
     2041 ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga tctcaatttt
     2101 aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat cgctgctggc
     2161 ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg cagcaccaat
     2221 attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc gctgcaataa
     2281 gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc tgtttactgt
     2341 ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat tattcgcctg
     2401 cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca ccccgcgagc
     2461 ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga caaacgcata
     2521 tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat acgccagctg
     2581 ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg cgcgctgggc
     2641 ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta agcgaccgaa
     2701 aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg tggctgaatc
     2761 gcctttaaga ttgccgctcc agctgccgct gccctcgcca ttgagctgtg ggtagcggtc
     2821 ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg ccatgcgcaa
     2881 atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat caacaaaaaa
     2941 ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt tctggctgag
     3001 tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca gtgaacaacc
     3061 agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag gctcataatt
     3121 tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg tgacaggaaa
     3181 atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta ctctagaatt
     3241 gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga aaagaaaacc
     3301 ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt ggccgacaac
     3361 gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat cctcgatatt
     3421 atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc caataaaggg
     3481 atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa ggggctggag
     3541 ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct ggcgcgggtg
     3601 cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca ggttgccgat
     3661 ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat cactctgacc
     3721 agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt gctgccccgc
     3781 tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa cgccattgat
     3841 gtggcggtga agctgctacg cggcaaaatc gacaacgact ttgagccgaa gctgattcaa
     3901 accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc catttcagcg
     3961 cccgttttcg ctggcaaccc gcctgacctt ttttatcagc caggccacca tcgcggcgtt
     4021 tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg agcaggacat
     4081 taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc ctgacgaaac
     4141 gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca acgtgttgat
     4201 ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc ctgatatccg
     4261 cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg tgtatctcct
     4321 ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac acagcaactg
     4381 gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt atacgctcta
     4441 catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga ataaacttat
     4501 tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg cggtacataa
     4561 aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct cgaaagatct
     4621 cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg tactgtcgtt
     4681 caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt tctcagcgga
     4741 tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg aaatcgccct
     4801 cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc tcgaagagct
     4861 gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg ataacaacca
     4921 gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag tgttcgattt
     4981 tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg acaaacgtca
     5041 ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt ctaatgccct
     5101 tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg atcaccaggt
     5161 gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac cgcgattgtt
     5221 tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta gcggtattgg
     5281 gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg taacgtcaga
     5341 tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca aacggcaact
     5401 tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg gtaaaaagct
     5461 gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc gttaatgtaa
     5521 ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg cttaaaagta
     5581 atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt ttttattgat
     5641 gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg ctacccgcga
     5701 atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat ggttccggcg
     5761 tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac gctcgactac
     5821 gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca acgtgatgtt
     5881 gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg tcctggtccc
     5941 accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt tgaacgtagc
     6001 gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat ttcaccgatg
     6061 gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat cccagtgtat
     6121 ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga gttttcagaa
     6181 ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc cagcgcaggc
     6241 gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg taacggtatt
     6301 gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct gaaagaaaaa
     6361 ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga acgcgagcag
     6421 cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga atatttcccc
     6481 gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat tgttttccag
     6541 ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct ctggcgcgac
     6601 cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat gatccagctt
     6661 ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct ggcatttcct ttctattttt
     6721 agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat ggttaaccgc
     6781 atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg cctgctttcc
     6841 gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg cgtgctgaaa
     6901 caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac gcatgacttc
     6961 cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct gctggaaaat
     7021 caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga aggtctacgc
     7081 ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc acaggcgctg
     7141 gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc gtcctcgctg
     7201 attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct gcgtctggaa
     7261 aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct ggcggccctg
     7321 acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca aattgtcaca
     7381 gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat gttgtacaat
     7441 gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg gcaatatgtg
     7501 ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa tcgttcagca
     7561 attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa gtttttggtc
     7621 accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca acgcgaatta
     7681 caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca ggttgcgcaa
     7741 ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt ctggtttact
     7801 gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca gcgcagaaga
     7861 gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata aggcgctgaa
     7921 ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata tacaccaaca
     7981 ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc attttggtca
     8041 tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag gagatgcccg
     8101 acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga ctgttgaaga
     8161 actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc gcagtgaagt
     8221 cgggcagaat gccctgcggc tggcacaatt acctgtcgcc agagcgcaac tgaacgatgc
     8281 gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc aacgggcaat
     8341 ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc agcagaacac
     8401 attaagcgcg gcagaacacc gtcagtggtt tgacgtgctt cttgccgggc agctggacga
     8461 tcggatcctg gcactgcaat cacaggggat cttcaccgat cctcagtcat atattactta
     8521 cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc tcattgaaaa
     8581 taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt tatctaaata
     8641 cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg ataaccgcca
     8701 gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg acgcagcgca
     8761 aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg ctgtcagcgt
     8821 ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc agcaagaacc
     8881 gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg agcagtcacg
     8941 cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc gtgtcagcca
     9001 gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag caacgccggc
     9061 gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt ggcaaagtca
     9121 gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg atatgtcgcc
     9181 ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca cgctacccgg
     9241 tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct ggcaacatcg
     9301 tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg cggcctggca
     9361 gaaaatcagt cttcacgaca tgagcaatga tgatctgctc gccgccgcca acaccgctca
     9421 ggcagcggga aatggcgcag ctcgcgatcg ctggcttcaa caggcagaac aacgtgagct
     9481 gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag ctcagccaga
     9541 actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca actcttacgt
     9601 tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga gtgatttgcg
     9661 cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg gttacgcctt
     9721 gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc ataaagggct
     9781 tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc tggatgacat
     9841 gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc aggcataacc
     9901 ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca tgaggaggtc
     9961 ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg cgcaatgagt
    10021 accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag ctacggacaa
    10081 ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct gctctcagtt
    10141 tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt gaaaaatccg
    10201 atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt cctcgccgtc
    10261 gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct gcgcgccagc
    10321 gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg ttcaggctgg
    10381 tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat tcaggcgtgg
    10441 acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac tattgagcct
    10501 tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc gcagcttggc
    10561 ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc ctggccgcac
    10621 aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca acgtaacgga
    10681 gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa gttcattttc
    10741 gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg tattatctgg
    10801 caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct gatgagtcag
    10861 ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg cgatgcattt
    10921 acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca acagactggc
    10981 ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca gaaacagtcg
    11041 tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca gcaagccaga
    11101 ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc ggaaattgac
    11161 gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt aaacaacgcg
    11221 cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt tttcgccgga
    11281 aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac cggagttaat
    11341 gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg tgaacgttat
    11401 ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt ttatgaactg
    11461 tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc agaaattgcc
    11521 tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc tctgcgctat
    11581 ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt cgggtacgac
    11641 ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta aaaagcatgc
    11701 taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa gcatagatcc
    11761 tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc agtgaacctt
    11821 gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat gctacgttta
    11881 cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt gaactctcaa
    11941 cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat tatctgttgt
    12001 ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctagcaacc ccggttatcg
    12061 cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc aagcagcaat
    12121 ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta ttgtctaact
    12181 ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc agcgtccgcg
    12241 acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg cgaattgtcg
    12301 ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac gaaaatacca
    12361 gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg ttattgatgg
    12421 ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg tcgtacttta
    12481 tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc aaacagcgac
    12541 aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa atgaagaaga
    12601 aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc gttgctctat
    12661 ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga attatacggt
    12721 caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac gaccgcgtct
    12781 gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg cgtctgaatc
    12841 aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg aaaattgtcc
    12901 gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga tactcatcct
    12961 atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca gattgtcctg
    13021 aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt tgatttaatc
    13081 attatggata tagacttgcc cggaacagac ggttttacct tcctg
//
LOCUS       DAZOYF010000108        13098 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZOYF010000108 DAZOYF010000000
VERSION     DAZOYF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.108"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHUOF_002893"
     CDS             complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHUOF_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR0611981.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="ACHUOF_002894"
     CDS             1131..1931
                     /locus_tag="ACHUOF_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0611982.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="ACHUOF_002895"
     CDS             2009..2779
                     /locus_tag="ACHUOF_002895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0611983.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHUOF_002896"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHUOF_002896"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR0611984.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACHUOF_002897"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACHUOF_002897"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR0611985.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="ACHUOF_002898"
     CDS             5034..5756
                     /locus_tag="ACHUOF_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0611986.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACHUOF_002899"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACHUOF_002899"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR0611987.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACHUOF_002900"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACHUOF_002900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR0611988.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="ACHUOF_002901"
     tRNA            7149..7225
                     /locus_tag="ACHUOF_002901"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="ACHUOF_002902"
     CDS             complement(7356..8402)
                     /locus_tag="ACHUOF_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFR0611989.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACHUOF_002903"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACHUOF_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACHUOF_002904"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACHUOF_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR0611990.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACHUOF_002905"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACHUOF_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR0611991.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="ACHUOF_002906"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="ACHUOF_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACHUOF_002907"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACHUOF_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137593242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3393 c         3484 g         3214 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgtgt
//
LOCUS       DAZOYF010000109        12957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZOYF010000109 DAZOYF010000000
VERSION     DAZOYF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.109"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..248)
                     /gene="yefM"
                     /locus_tag="ACHUOF_002908"
     CDS             complement(<1..248)
                     /gene="yefM"
                     /locus_tag="ACHUOF_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR0611992.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     misc_feature    548..671
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            718..1617
                     /gene="hisG"
                     /locus_tag="ACHUOF_002909"
     CDS             718..1617
                     /gene="hisG"
                     /locus_tag="ACHUOF_002909"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR0611993.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1623..2927
                     /gene="hisD"
                     /locus_tag="ACHUOF_002910"
     CDS             1623..2927
                     /gene="hisD"
                     /locus_tag="ACHUOF_002910"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR0611994.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2924..3994
                     /gene="hisC"
                     /locus_tag="ACHUOF_002911"
     CDS             2924..3994
                     /gene="hisC"
                     /locus_tag="ACHUOF_002911"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR0611995.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3994..5061
                     /gene="hisB"
                     /locus_tag="ACHUOF_002912"
     CDS             3994..5061
                     /gene="hisB"
                     /locus_tag="ACHUOF_002912"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR0611996.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5061..5651
                     /gene="hisH"
                     /locus_tag="ACHUOF_002913"
     CDS             5061..5651
                     /gene="hisH"
                     /locus_tag="ACHUOF_002913"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR0611997.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5651..6388
                     /gene="hisA"
                     /locus_tag="ACHUOF_002914"
     CDS             5651..6388
                     /gene="hisA"
                     /locus_tag="ACHUOF_002914"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR0611998.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6370..7146
                     /gene="hisF"
                     /locus_tag="ACHUOF_002915"
     CDS             6370..7146
                     /gene="hisF"
                     /locus_tag="ACHUOF_002915"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR0611999.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7140..7751
                     /gene="hisIE"
                     /locus_tag="ACHUOF_002916"
     CDS             7140..7751
                     /gene="hisIE"
                     /locus_tag="ACHUOF_002916"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR0612000.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="ACHUOF_002917"
     CDS             complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="ACHUOF_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR0612001.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="ACHUOF_002918"
                     /pseudo
     CDS             complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="ACHUOF_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="ACHUOF_002919"
     CDS             complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="ACHUOF_002919"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR0612002.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11945..12259)
                     /locus_tag="ACHUOF_002920"
     CDS             complement(11945..12259)
                     /locus_tag="ACHUOF_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612003.1"
                     /translation="MKKASEYSELYGDNLIQLGLEDGIYFYKGMAIGDVFGLARYSDW
                     TISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYPKFKVLMDTPNGILIA
                     IK"
     gene            complement(12301..>12957)
                     /locus_tag="ACHUOF_002921"
     CDS             complement(12301..>12957)
                     /locus_tag="ACHUOF_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612004.1"
                     /translation="NKTQNELIFLCGFFIGIACGVKLFGAIYGAIIFVILIAKKRNIQ
                     TFLIFSLATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQTAEQARHGI
                     SPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWFSVTQGDRY
                     LSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILNSNIFILNT
                     "
BASE COUNT         3356 a         3242 c         3169 g         3190 t
ORIGIN      
        1 tcaataatgt ccttttccgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
       61 ctccgggcgt tagcggggga acgcagtaga taagccgtct cttccagcga attgtattct
      121 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taatgaggat cggggcatga
      181 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
      241 gtacgcatgt aaatctccta ttttgtacag ttcattgtac aatgataggt gttaattaac
      301 tatttattaa ttagtttgta gatcaagaca ttgccagcga gacgaaaaat cagactttgc
      361 tctttttggt gctgtcaagt tagaggacag tcctcttagc cccctccttt ccccgctcat
      421 tcattaaaca aatccatcgc cataaaatat ataaaaaagc ccttgctttc taacgtgaaa
      481 gtggtttagg ttaaaagaca tcagttgaat aaacattcac agagattttt atgacacgcg
      541 ttcaatttaa acaccaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca
      601 ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg
      661 ggctttttta ttgcgcggtt gttaacggtt cagacaggtt taaagaggaa taagaaaatg
      721 acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca
      781 cgcgaattac tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg
      841 atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgatgacat tccctgtctg
      901 gtaatggacg gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg
      961 cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccctgcgtcg tctggatttc
     1021 ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggacgg tccgctctcc
     1081 ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag
     1141 aaaggtatct cttttaaatc ctgcttactg aacggttctg tcgaagttgc gccgcgtgcc
     1201 gggctggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga agctaacggc
     1261 ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg cgatggcgaa
     1321 atggaagaat ctaaacagca actgatcgac aagctactga cccgtattca gggtgtgatc
     1381 caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgcct ggatgaagtc
     1441 atcgccctgc tgccaggtgc cgaacgccca accattctgc cgctggcggg tgatcaacag
     1501 cgcgtagcga tgcacatggt gagtagcgaa accctgttct gggaaacgat ggaaaaactg
     1561 aaagcactgg gtgccagttc aatcctggtc ctgccgattg agaagatgat ggagtgatcg
     1621 ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc
     1681 tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt aacgatattc
     1741 tcgataacgt gaaaacgcgt ggcgatgagg ccctgcgtga atacagcgcg aagtttgata
     1801 aaaccacggt taccgcgctg aaggtgtctg ctgatgagat cgccgccgcc agcgaacgcc
     1861 tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gagtattgaa accttccaca
     1921 ctgcgcaaaa actgccgccg gtagatgtag aaacgcagct aggcgtgcgt tgccagcagg
     1981 tcacgcgccc ggtggcttca gtgggtttgt atattccggg cggctccgcc ccgctcttct
     2041 caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt
     2101 gctcaccgcc gccgattgcc gatgaaattc tttatgcggc gcagctgtgc gatgtgcagg
     2161 acgtgtttaa cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt acggaatctg
     2221 tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa gcaaaacgcc
     2281 aggtaagcca gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc
     2341 tggtgattgc tgacagcggc gctacgccgg attttgtggc ttctgatttg ctttctcagg
     2401 ctgaacacgg ccctgactca caggtaattt tactgacccc cgatgctgat atggcgcgtc
     2461 gcgttgccga ggccgtcgaa cgccaactgg cagaactgcc gcgtgccgaa accacccgcc
     2521 aggcactgaa cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtggagatct
     2581 ccaaccagta cggcccggag cacctgatca ttcagacccg caacgcccgc gaactggtcg
     2641 atggcatcac cagcgccggt tcggtatttc ttggtgactg gtcaccggaa tcggcaggcg
     2701 actacgcctc cggcaccaac cacgtgctgc cgacttacgg ttacaccgcc acctgttcca
     2761 gcctcgggct ggcagatttc cagaaacgca tgaccgtaca ggaactgtcg aaagaaggtt
     2821 tctccgcgct ggcttcgacc attgaaacac tggccgccgc cgagcgcctg accgcccaca
     2881 aaaatgccgt tactttgcgt gttaacgtcc ttaaggagca agcatgagca ccgtgactat
     2941 tactgattta gcgcgtgaaa acgtccgcaa cctgacaccg tatcagtcgg cgcgtcgtct
     3001 gggcggtaac ggcgacgtct ggctgaacgc caacgaatat cccacagccg tggagtttaa
     3061 gcttactcag caaacgctca accgctaccc ggaatgccag ccaaaagcgg tgattgaaaa
     3121 ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg gcgcggacga
     3181 aggtattgaa ctgctgatcc gcgctttttg cgaaccgggt aaagacgcca tcctctactg
     3241 cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggtgtcg aatgccgcac
     3301 agtgccgacg ctggaaaact ggcaactgga cttacagggc atttccgaca agctggacgg
     3361 cgtaaaagtg gtctatgttt gcagccccaa caaccccacc gggcaactga tcaatccgca
     3421 ggattttcgc accctgctgg agctaacccg cggtaaggcg attgtggttg ccgatgaagc
     3481 ttatatcgag ttttgcccgc aggcatcgtt agctggctgg ctggcggaat atccgcacct
     3541 ggcgattttg cgtacccttt cgaaagcctt cgcgttggcg gggcttcgtt gcggatttac
     3601 gctggcaaac gaagaagtca tcaacctgct gatgaaagtg atccccccct acccgctctc
     3661 gacgccggtt gccgacattg ccgcacaggc gttaagcccg cagggaatcg tcgccatgag
     3721 cgaacgggtg gcgcaaatta ttacagaacg cgaatacctg attgccgcac tgaaagagat
     3781 cccctgcgtg gagcaggttt tcgactctga aaccaactac attctggcgc gctttaaagc
     3841 ctccagtgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa
     3901 acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg
     3961 cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat
     4021 cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa
     4081 ctcgcctttg aaccgggcgt gatcccggaa ttgctgaagc tgcaaaaagc gggctacaag
     4141 ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccgca agcggatttt
     4201 gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa
     4261 gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa
     4321 ctggtggagg gttatctggc tgagcaagcg atggatcgtg ccaacagtta tgtaattggc
     4381 gatcgcgcga ccgacattca actggcggaa aacatgggca ttaatggttt acgctacgac
     4441 cgcgaaaccc tgaactggcc aatgattggc gagcaactca ctaaacgaga ccgttacgcc
     4501 catgtagtgc gcaacaccaa agagacgcag attgacgtcc aggtgtggct ggatcgcgaa
     4561 ggtggcagca agattaacac cggcgttggc ttctttgatc acatgctgga tcagatcgcc
     4621 acccacggcg gtttccgcat ggaaatcaat gtcaaaggcg acctctatat cgacgatcac
     4681 cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc cctcggcgac
     4741 aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc
     4801 gcactggata tctctggtcg cccgcacctg gaatataaag ccgagtttac ctaccagcgt
     4861 gtgggcgatc tcagcactga aatgatcgaa cacttcttcc gttcgctctc ttacaccatg
     4921 ggcgtgacac tacacctgaa aaccaaaggt aaaaacgatc atcaccgtgt agaaagcctg
     4981 ttcaaagcct ttggtcgtac cctgcgccag gccatccgcg tcgaaggcga caccctgccc
     5041 tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga tactggctgc gccaacctga
     5101 actctgtgaa gtctgccatt gcgcgccacg gttatgaacc caaagtcagc cgtgacccgg
     5161 acattgtgtt gctggccgat aaactgtttt tacccggcgt cggcaccgca caagcagcga
     5221 tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtgc
     5281 tgggcatctg cttagggatg caactcctgg gacgacgcag cgaagagagc aatggcgtcg
     5341 acttgctggg catcatcgac gaagacgtgc cgaaaatgac cgactttggt ctgccactgc
     5401 cacatatggg ctggaaccgc gtttatccgc aggcaggcaa ccgcctgttt caggggattg
     5461 aagacggcgc gtacttttac tttgttcaca gctacgcaat gccagtcaat ccgtggacca
     5521 tcgcccagtg taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg
     5581 gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg aaaaacttcc
     5641 tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca
     5701 tcagggcgat tacggcaaac aacgcgatta cggtaacgac ccgctgccgc gcttacagga
     5761 ttacgctgcg caaggtgccg aagtgttgca cctggtggat ctgaccgggg caaaagatcc
     5821 ggctaagcgt caaatcccgc tgattaaaac cctggtcgcg ggcgttaacg tcccggtgca
     5881 ggttggcggc ggtgttcgta gtgaagaaga cgtggcggcg ttactggaag ctggcgttgc
     5941 gcgcgtggtg gtcggttcca ccgcagtgaa atcaccggac gtagtgaaag gctggtttga
     6001 acgtttcggt gccgatgcgc tggtgctggc actggatgtc cgtattgacg agcaaggcaa
     6061 caagcaagtg gcagtcagcg gctggcaaga gaactcgggc gtttcactgg aacaactggt
     6121 ggaaacctat ctgcccgtcg gcctgaaaca tgtgttgtgt accgatatct cgcgcgacgg
     6181 cacgctggcg ggatctaacg tctctttata tgaagaagtg tgtgccagat atccgcaagt
     6241 ggcgtttcag tcctccggcg gcattggcga cattaacgat gttgctgccc tacgtggcac
     6301 tggcgtgcgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca ccgtgaagga
     6361 ggccatcgca tgctggcaaa acgcataatc ccgtgtctcg acgttcgtga tggtcaggtg
     6421 gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc actggcaaaa
     6481 cgctacgccg aagaaggtgc agatgaactg gtgttctacg atatcaccgc ttccagcgat
     6541 ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga cattccgttt
     6601 tgtgtggcgg gtgggattaa gtctctggaa gatgccgcga aaattctttc ctttggcgcg
     6661 gataaaattt ccatcaactc ccctgcgctg gcagacccaa cattaattac tcgcctggcc
     6721 gatcgctttg gcgtgcagtg tattgtggtt ggtattgata cctggtacga cgccgaaacc
     6781 ggtaaatatc atgtaaatca atataccggc gatgaaagcc gcacccgcgt cactcaatgg
     6841 gaaacgctcg actgggtaga ggaggtgcaa aaacgcggcg ccggagaaat cgtcctcaac
     6901 atgatgaatc aggacggcgt gtgtaacggt tacgacctca agcagctgaa aaaagtgcgt
     6961 gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga acacttcctg
     7021 gaagccttcc gcgatgccga cgttgacggt gcgctggcag cttccgtatt ccacaaacaa
     7081 ataatcaata ttggcgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata
     7141 tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacggt ctgatgccgg
     7201 tgattgtgca acacgcggta tctggcgaag tgttaatgct gggctacatg aacccggaag
     7261 ccttagacaa aaccatcgaa agcggtaaag tcaccttctt ctcgcgcact aaacagcgac
     7321 tgtggatcaa aggcgaaacc tcgggcaatt ttctcaatgt ggtaagtatt gccccggact
     7381 gcgacaacga cacattactg gtgctggcga atcccatcgg gccaacctgc cacaaaggca
     7441 ccagtagctg cttcggcaac accgctcacc agtggctgtt cctgtatcaa ctggaacaac
     7501 tgctcgccga acgtaaatat gccgacccgg aaacctccta caccgccaaa ctgtatgcca
     7561 gcggcactaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc gcactggcag
     7621 caacggtaca tgatcgcttt gagctgacca acgaggcatc tgacttgatg tatcacctgc
     7681 tggtgctgtt gcaggatcag gatctggatt taacgacagt aattgagaac ctgcataaac
     7741 ggcatcagtg agttacgggc tgagcaggtg tgatgttgtt gtcacacccg gagccattgc
     7801 ccgatgcgca aacttatcgt gcctacacat tatctcaaca cacgtaggcc ggataagatg
     7861 cgccagcatc gcatccggca aaaaaaacgg gcatggtgtc accaccctgc ccgtttctct
     7921 taaatgcaca ataatattac ttcgcgttgt aattacgcag agcgttacgc cccaacacaa
     7981 tccccgcgcc aaccatgccg cccagaagca ctgccagaat caaggtgatt gcctttttcg
     8041 gactatcgcg acgaataggt aacgtcggtt tcatcacata gcggtaagca tgaatatcaa
     8101 gatcatcaaa ctttaatttt tcaatatcca acaggttttg acgtgtctga tagtagtttg
     8161 gtgagaacac caacggacga gtcgcttcat gcttaatcat cgactccagc gcttcgctcc
     8221 ctagaaggaa caacgtatct tgcgtcacat cttcagtctg ctgaacctgt ggctttgtca
     8281 cctgcgcctg attcgcatac tgcaacgctt cctgaatctg acggatacgc agatctttct
     8341 gctcctgcgc gaccacttcc tgggttctta aagagtcctg caagtttttc cgtcccagag
     8401 caatgttgtc cttgagatcc ttttctagct cttgattcac tttatcatca acttgctgaa
     8461 tgtattgggc caacttcatt tgtgcgccct ctgcagtttg cccaacataa gaaacagtca
     8521 atggtaattg ctggttctta acagaaggtt cgatggtaag tttttctggc tcttcctgat
     8581 tatccagcgt ttctgctaat gcagagaagg cagaactgaa gcgaccaatt aacgtctcct
     8641 gcaaatccga tactttcggt gcagcctgac catagataac attcatggca ttgttatagc
     8701 cagcaatttg ccccacgtcg ggctgagtga taattgctgt tgacgtccat ttctccttcg
     8761 ctactgccaa ataaccaata gccagggcaa tagccacaat gacggaaatg ataattgtca
     8821 tcttgccacg ccacaactgc actagtaaat caatcaaatc aatctgttcc gggtcatggt
     8881 tttgcccaga aacattatta ttttctactc tcatcattac cctaactgac ggaaatttcc
     8941 aagtccgcat agtttagcga taatccccgt gaatgctata ggataagtga taaaaataat
     9001 gaattattgc ggaggtaaca aaagacaatc agggcgtaaa tagccctgat aacaagatgt
     9061 tagtcgctgc caaagagatc gcgggtgtag accttatctg ccacatcctt aagctcttct
     9121 gccatacggt tggagataat gacgtcggct tgttgtttga aggtggcgag atcacgttcc
     9181 aggcgagagt tgaagaatga gtcttctttc atcaccggct cgtagatgat cacttcaacg
     9241 cctttcgcct tgatacgctt cataatcccc tgaatagaag acgcacggaa gttatctgac
     9301 ccgctcttca taatcagacg ataaataccc accacttgcg gtttgcatcg acaattgccg
     9361 agatcaggtt attcggcaca gactggtagt tcgccagtaa ctgcttggta tctttcggca
     9421 gacaataacc accataacca aacgacggat tgttgtaatg gttgccaata cgcggatcga
     9481 gacaaacgcc ttcgataatc tggcgagtat tcagacctaa actttctgca taactatcca
     9541 gttcattaaa gtatgctacg cgcatcgcca gataggtatt agcgaaaagt ttaatcgctt
     9601 ctgcttcagt ggagtcggta aacagggtcg ggatattttg cttaatcgcg ccttcctgta
     9661 acaacgcagc aaaacgttct gcgcgttctg aacgctcacc gatgacaata cgtgacggat
     9721 ggagattatc gtaaagggct ttaccttcgc ggaggaattc cggagagaaa ataatatttt
     9781 cagtacgata tttcttatgc atcgctgcgg taaaaccaac gggaaccgtt gatttgatga
     9841 ccataaccgc ataaggattt atctcaacta cgtctttaat tactgattct acactggatg
     9901 tattgaaata attagtttta ggatcatagt cggttggagt ggcgatgatg acataatcag
     9961 catcccggta ggcttcattt ttatctaatg tggcattaaa gtgtatttta tctgattgca
    10021 aaaactgctg aatttcctta tcaacaatag gagatatccg atcattcagc atagcaacgc
    10081 gtgacggtaa aatatctaat gccacaacct catgattttg tgcgattaga agcccgtttg
    10141 acaagcctac atagccagta ccggaaatgg tgattttcat ttcatgctct cagaattaac
    10201 ttaactgtga atcatgatgt ttttagcatc gtgataagat gctaaaagtt ttaacgctac
    10261 gttgtcagat ggtcaacgca acgaaaaaca attcagattt tctttataag aatagctgat
    10321 gtttattaag ttaatattaa gcagtgaaat ttagtctata agatatttgg caaaaaaaag
    10381 cccggtgcaa tatacgccgg gcctcaattt tattgttggt taaatcagat taatccagcc
    10441 attcagtatg gaacacacct tctttatcaa tgcgcttata agtatgcgcc ccgaaatagt
    10501 cacgctgtgc ctggatcagg ttcgcaggca gaaccgcggc acggtagcta tcgtaatagg
    10561 caaccgcagc ggcgaaggtc ggaaccggga taccgttctg tactgcataa gcgacgacat
    10621 cgcgcagcgc ctgctggtag tcatcggcaa tttgcttgaa gtacggggcc agcagcaggt
    10681 tagcgatctg cggattttcg gcataagcat cggtgatttt ctgcaggaac tgcgcacgga
    10741 tgatgcagcc agcacggaaa atcttcgcga tttcgccgta gttcagatcc caattgtatt
    10801 cttcagacgc agcacgcagc tgagagaagc cctgagcgta agaaacgatt ttgcccagat
    10861 acagcgcact gcgaactttt tcgatgaact cagccttgtc gcctgctgac tgagcttgcg
    10921 gaccagagag aactttagat gcggcaacgc gctgatcttt cagagaagag atataacgtg
    10981 caaacacaga ctcggtaatc agcgacagcg gttcgccgag atccagcgcg ctctggctgg
    11041 tccatttacc ggtgcctttg tttgctgctt catccagaat cacatcaacc aggtagttac
    11101 cgtcttcatc ttttttggtg aagatatctt tagtgatgtc gatcaggtag ctgctaagtt
    11161 caccgttatt ccactcggtg aaagtctggg ccagttcttc attagataga ttcaggccac
    11221 ctttgagcag agaatacgct tcagcaatga gctgcatatc tccgtattca ataccattgt
    11281 gaaccatctt cacataatgg cctgcgccgt cggcaccaat ataagtaacg catggctcgc
    11341 cgtcttctgc cacggcagcg attttagtca ggataggcgc aaccagctca taagcctctt
    11401 tctgcccgcc aggcataata gaaggacctt tcagcgcgcc ctcttcaccg ccggaaacac
    11461 cggtaccgat gaagttaaag ccctctgctg aaagctcacg attacgacga atggtgtcct
    11521 ggaagaaggt attaccacca tcaatgatga tgtcaccttt atcgagatat ggtttcaggg
    11581 aatcaatcgc agcatccgtg cctgcacctg ctttcaccat taacaggatg cgacgaggcg
    11641 tttccagaga ttcaacaaac tctttcaccg tatagtaagg agccagtttc ttgcctggat
    11701 tttcagcaat cacttcttcc gtcttttcgc gggagcggtt gaaaatagag acggtataac
    11761 cacgactttc gatgttgagc gcaaggttgc gccccatcac tgccataccg actacgccga
    11821 tctgttgctt tgacattgtt tactcctgtc ttctatatta ttaaccgtct attaaccgtt
    11881 atattattaa ccgtcgtgat tttcatcttc taatcaagga atacatatgg ttacagcatg
    11941 cttcttactt tatagcgata agtatgccat tgggtgtatc cataagaact ttgaattttg
    12001 gatacttttc tggattaaaa ttagcatagc ttcgtttaga tataacaata aacgaactat
    12061 taaaagattt catcttctct attaaatcat cctgtgggat tacctcacac tcgggattac
    12121 taatagtcca atcactatat cttgctaatc caaagacgtc accaattgcc atccctttat
    12181 agaagtaaat accatcctcc aagcctaatt ggataagatt atctccatat aactcactat
    12241 attcacttgc tttcttcatc agttcatagc tatagcgact ttctaagcgt gaattaaaat
    12301 tcaagtattc agaataaaaa tatttgaatt caaaatacaa cgatcgcccc gaaaaaaaat
    12361 aatacacaca aggataaaat atcgtcccaa cttgtaattt gtaatgttga tatcttttat
    12421 taaacttgca attgagatcg caacctgcaa tactgacaat gggtaaatgg gtgataaata
    12481 cctgtcacct tgcgtaacag aaaaccaaaa taagacatat gttaaaacaa tcgagaaaaa
    12541 tacccaaagt attttattat tcctaagtgt aaagacagac agtatagcat aaaaaacaac
    12601 atcactgcca actttattaa ttgcttgttg caaatctaat gtggcaggac ttataccatg
    12661 tcgagcttgt tctgctgttt gaacttgtag atcaatttca ttccataggt agtgaccaaa
    12721 tatatttcct ccagcaggac taaaaggatc accagagatc caaaaactac ggacatacca
    12781 ataaatgcca ccaagaattg ttgccaagct aaaaataaga aatgtctgta tgtttctctt
    12841 tttcgctatc aatataacaa atatgatggc tccatatatg gcaccaaata gttttacgcc
    12901 acaagcaatt ccaataaaaa aaccacataa aaaaattaat tcattttgtg ttttatt
//
LOCUS       DAZOYF010000110        12908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZOYF010000110 DAZOYF010000000
VERSION     DAZOYF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.110"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACHUOF_002922"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACHUOF_002922"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR0612005.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="ACHUOF_002923"
     CDS             complement(1806..3224)
                     /locus_tag="ACHUOF_002923"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0612006.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="ACHUOF_002924"
     CDS             complement(3378..3722)
                     /locus_tag="ACHUOF_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR0612007.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACHUOF_002925"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACHUOF_002925"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR0612008.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5653)
                     /locus_tag="ACHUOF_002926"
                     /pseudo
     CDS             complement(4712..5653)
                     /locus_tag="ACHUOF_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="ACHUOF_002927"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="ACHUOF_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR0612009.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="ACHUOF_002928"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="ACHUOF_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR0612010.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACHUOF_002929"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACHUOF_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR0612011.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="ACHUOF_002930"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="ACHUOF_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR0612012.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="ACHUOF_002931"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="ACHUOF_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR0612013.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="ACHUOF_002932"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="ACHUOF_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR0612014.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACHUOF_002933"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACHUOF_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFR0612015.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11368..12483
                     /gene="ydeE"
                     /locus_tag="ACHUOF_002934"
                     /pseudo
     CDS             11368..12483
                     /gene="ydeE"
                     /locus_tag="ACHUOF_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="ACHUOF_002935"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="ACHUOF_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFR0612016.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            12705..12808
                     /gene="mgtT"
                     /locus_tag="ACHUOF_002936"
                     /pseudo
     CDS             12705..12808
                     /gene="mgtT"
                     /locus_tag="ACHUOF_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
BASE COUNT         3401 a         3299 c         2950 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatga
//
LOCUS       DAZOYF010000111        12700 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZOYF010000111 DAZOYF010000000
VERSION     DAZOYF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.111"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..145
                     /locus_tag="ACHUOF_002937"
     CDS             <1..145
                     /locus_tag="ACHUOF_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612017.1"
                     /translation="NTLEKDENSPDNGFEVGEYNTYEAYNSENQYFTREDYTYNYDLL
                     NAI"
     gene            complement(642..1018)
                     /gene="rnpB"
                     /locus_tag="ACHUOF_002938"
     ncRNA           complement(642..1018)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHUOF_002938"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1051..2196)
                     /gene="garK"
                     /locus_tag="ACHUOF_002939"
     CDS             complement(1051..2196)
                     /gene="garK"
                     /locus_tag="ACHUOF_002939"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR0612018.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(2293..3183)
                     /gene="garR"
                     /locus_tag="ACHUOF_002940"
     CDS             complement(2293..3183)
                     /gene="garR"
                     /locus_tag="ACHUOF_002940"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR0612019.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(3213..3983)
                     /gene="garL"
                     /locus_tag="ACHUOF_002941"
                     /pseudo
     CDS             complement(3213..3983)
                     /gene="garL"
                     /locus_tag="ACHUOF_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3999..5333)
                     /gene="garP"
                     /locus_tag="ACHUOF_002942"
     CDS             complement(3999..5333)
                     /gene="garP"
                     /locus_tag="ACHUOF_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR0612020.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5708..7279
                     /gene="garD"
                     /locus_tag="ACHUOF_002943"
     CDS             5708..7279
                     /gene="garD"
                     /locus_tag="ACHUOF_002943"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR0612021.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(7331..8155)
                     /gene="agaR"
                     /locus_tag="ACHUOF_002944"
     CDS             complement(7331..8155)
                     /gene="agaR"
                     /locus_tag="ACHUOF_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR0612022.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            8404..9684
                     /gene="kbaZ"
                     /locus_tag="ACHUOF_002945"
     CDS             8404..9684
                     /gene="kbaZ"
                     /locus_tag="ACHUOF_002945"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR0612023.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9707..10180
                     /gene="agaV"
                     /locus_tag="ACHUOF_002946"
     CDS             9707..10180
                     /gene="agaV"
                     /locus_tag="ACHUOF_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR0612024.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10191..10970
                     /gene="agaW"
                     /locus_tag="ACHUOF_002947"
     CDS             10191..10970
                     /gene="agaW"
                     /locus_tag="ACHUOF_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR0612025.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10960..11769
                     /gene="agaE"
                     /locus_tag="ACHUOF_002948"
     CDS             10960..11769
                     /gene="agaE"
                     /locus_tag="ACHUOF_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR0612026.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11856..12290
                     /gene="agaF"
                     /locus_tag="ACHUOF_002949"
     CDS             11856..12290
                     /gene="agaF"
                     /locus_tag="ACHUOF_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR0612027.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12287..>12700
                     /locus_tag="ACHUOF_002950"
     CDS             12287..>12700
                     /locus_tag="ACHUOF_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR0612028.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         3073 a         3399 c         3205 g         3023 t
ORIGIN      
        1 taataccctc gagaaagacg aaaattctcc ggacaatggc tttgaggtcg gggaatataa
       61 cacatatgaa gcatataact cagagaatca atattttacc agagaggact atacgtataa
      121 ttacgacctt ttaaatgcaa tatagactta ataacttctt acaaagtcta ttattaatac
      181 cgtagatatt catcttaccc tgtgcaccaa aagcaaaaac ttatcgacgc gggtttgaaa
      241 ctgatggcaa ccgcaaaaat gcctgatgcg ctacgcttat caggcctacg ccatctctgc
      301 aatatattga atttgcgtgc ttttgtaggc aggataaggc gttcacgcag catccggcat
      361 gaataaagcg cactcaacaa tctgaaaccc gccggaacgg tttattacgt acatcaggtg
      421 aaactgaccg ataagccgct ttcttttggg tatagtgtcg tggacagtca ttcatctttc
      481 tgcccctcca aaagcaaaaa cccgccgaag cgggttttta cgtatatcag gtgaaactga
      541 ccgataagcc gctttctttt gggtatagcg tcgtggacag tcattcatct ttctgcccct
      601 ccaaaagcaa aaacccgccg aagcgggttt ttacgtaaaa caggtgaaac tgaccgataa
      661 gccgggttct gtcgtggaca gtcattcatc taggccagca atcgctcact ggctcaagca
      721 gcctacccgg gttcagtacg ggccgtacct tatgaacccc tatttggcct tgctccgggt
      781 ggagtttacc gtgccacgga ctgttaccag ccgcgcggtg cgctcttacc gcaccctttc
      841 acccttacct gatcccgctt gcacgggcca tcggcggttt gctctctgtt gcactggtcg
      901 tgggtttccc ccccaggcgt tacctggcac cctgccctat ggagcccgga ctttcctccc
      961 ctccacccgt ctcccccgaa gaggacgacg acgaagcggc gactgtctgg tcagcttcgg
     1021 cgcgcagtat agagggtttg cgcgcccttg tcaccccgtg ttgcgcattc caatcgccag
     1081 tgtcgcggca atattacgtg aagcacggca gatattgtca taagccccgc ggaatgcttc
     1141 gtccaacgta cctatgctgg tcaatacgct gaagaccgca tcaatgccat gctgatgtac
     1201 aacgccaaca tcatcggtca ggctacccgc aatgccaatc accggtttat ggtacttctt
     1261 cgccacgttt gcgacgccaa tcggtacctt gccgtgaata ctctggctgt caatacgccc
     1321 ttcaccggtg atcaccagcg tacaatcgtg aatatgttcc tccagattca gcgccgtagt
     1381 gacgatttca ataccacttt tcagttccgc accaagaaac gccatcagcg ccgcacccat
     1441 accgcccgca gctcctgcgc cggggacatc ttttacatca acatgcagcg cttttttaat
     1501 gacatcggca tagtgagaga ggttattgtc cagctcaaca atcatcgctt cactggctcc
     1561 cttttgtggg ccaaagatgc gcgatgcgcc gttatcgccc accagcggat tggtgacatc
     1621 acaagcgacg cgaatgacgc aatcttttaa gcgcggatcg aggccggaaa tatcaatatc
     1681 attcagagta ttaagactac cgccgccaaa accaatttca ttgccgttgg cgtcgcataa
     1741 tttcgccccc agcgcctgta ccatgcctgc gccgccatca tttgtagcgc taccgccaat
     1801 gccgataata atgtttgtcg cgccgctctc cagcgcctgc aggattaact cgcctgtgcc
     1861 gcgtgaagtg gtcacgagtg gatcgcgttt ttccgcaggt accagctcca gcccactggc
     1921 cgccgccatt tcaataaacg cggttttgcc atcgccggag atcccccaac tggcattcac
     1981 tttctcgccc agcggccctg taacccaggc gtgacgttca gaaccctggg tggctgcaat
     2041 catcgcttcc accgttcctt cgccaccgtc ggcaaccgga atagaaacgt actgtgcatc
     2101 aggaaaaatt tcccgaaatc ctttttctat cgcctgcgca acctcgctgg cagataaact
     2161 ttctttataa gagtctgggg cgattacgat tttcatacct atgcctgtta ccacatgacg
     2221 ccggagggcg tttctcttat tcggcctgga ttccaggccc ggattgcaat acgccatccg
     2281 ggcacgacgt cattaacgag taacttcgac tttcgccagt ttttcgtagt agcacaccag
     2341 ggcgctatga tccgccgttc ctaaaccatc tgctcgcagt gcctgcatca tctccataac
     2401 cgcagctgtg agcggcagtt gcgcgccgac gccgtgggaa gtatccagcg catttgccag
     2461 atccttaata tgcagatcaa tacggaagcc cggcttgaag ttgcggtcca tcaccatcgg
     2521 cgctttggca tccagcacgg tactgcccgc cagtccaccg cgaattgcct gatacaccag
     2581 gtccgggtta acgcccgctt tagttgccag cgttaacgct tctgacatcg cggcaatatt
     2641 cagcgccaca atgacctgat tcgccagttt ggtgacgtta cctgcaccaa ttgccccggt
     2701 atgcaccacg gaacccgcca ttgctttcat caaatcatag tatttgtcga aaatagcctt
     2761 gtcgccgccc accatcactg acagcgtacc gtcgatggct ttcggttcac cgccgctcac
     2821 cggagcatcc agcatatcaa tgcctttcgc tttcagcgct tcgctgattt cacggcttgc
     2881 cagcggtgcg atagaactca tatcgatcaa taccgtacct ggcttcgcgc cttcaataat
     2941 gccattctca cccagcgcca cctctttcac atgaggggag tttggcagca tggttatgat
     3001 gacgtcgcac tgttcagcga tcgctttagc cgtagaggct gtttctgcac ctgcagcaat
     3061 cacgtcagca atagcttctg ggttacggtc agcaaccacc agcgagtaac ctgctttcag
     3121 aaggttttta ctcattggtt tacccataat ccccaggcca ataaaaccaa ctttcatagt
     3181 catatcaatc atctctcttg ttgcggtggt ggttattttt taaaggtatc agccagttta
     3241 tgagtggcag agcggaagac gccgagatcg ctgccgacag ccacaaacgt cgcgccccat
     3301 tccagataac gacgcgcatc ggcttcgacc ggcgcgagga taccgctggg tttgccgtgc
     3361 gcgctggcac ggttaaaaat gtgctgaatt gctttttgta catccgggtg tgatgcattg
     3421 ccgagatggc ctaatgccgc ggccagatcg ctggggccga cgaagatgcc gtctacgcct
     3481 tcggtagcgg caatggcatc gacgttatct acgccctgct gactttctat ctggaccaga
     3541 atagtgatgt tcttgttcga ctgagcgaaa taatccgcca cggtgccaaa catattggcg
     3601 cggtgagaaa cggagacgcc gcgaatgcct tccggtgggt aacgggttga tgccaccgcc
     3661 tgctctgctt cctcttttgt ttctacaaaa ggaatcagga agttatagaa accgatatcc
     3721 agaagacgct taataatttc cggctcgttg gtcggcactc acactactgg cgcgctggcg
     3781 ctgcctttca aggccattaa ctgcggaata aacgtggaga tatcgtttgg cgcatgttcg
     3841 ccatccagca ccagccagtc aaacccagcc aaaccaagaa cttcagtgct aatcgggtta
     3901 gagagtgctg accagcaacc aatttgtacc tgtttcgcag ccagtgcggc tttgaattta
     3961 ttcgggaaaa catcgttatt catcgcttat acctttgctt atttctgcaa ttccatacgt
     4021 ttaatgtcgc caactacgaa gaggtagcag accatcgcca tcagcgctga acatcccacg
     4081 aaaaccagtg ctgcattgaa ggagtgcagt tcacttacca ggtagccaat caccagtgga
     4141 gtgacaatgg aggcaacatt gccaaagacg ttaaagacgc cgccacagag gccaacaatc
     4201 tctttcggcg cggtgtcaga aatcaccggc cagcccagag caccaaatcc tttgccaaaa
     4261 aaagccagcg ccatcagcat gaccaccagc gtggtgttgt tggtgtagtt acataagatg
     4321 atggtggaag ccagcaacat tcccagcaca atcggtagct tacgtgccag ggtcagggat
     4381 aaaccgcgtt tgatcagata atccgagaag acacctccca gcacgccgcc cgcaaaacca
     4441 cacagtgccg gaatcgaggc gaccagaccc actttcagaa tcgacatgcc tttttcctgc
     4501 accagataaa tcgggaacca ggtgaggaag aaccaggtga tggtgttgat aaaatattgt
     4561 ccgaaaaata cgcccaacat catgcggtta gagagcaatt gcttgatgta atgcagtttg
     4621 ggaccgcttg ctgccgcgct gcccggcttt ttgtggtcca tatcgaccac cgcgccattt
     4681 tcagagataa acttcagctc ttcctcagac atacgtgggt gatctgtcgg gttatgaatc
     4741 aacttgatcc acagcgccgt cagcacaaaa caaatcaccc ccataacggt aaagacgtgc
     4801 tcccatcccc aggcgaaagt cagccagcca agcagaggcg aaaagagcgc cagcgagaaa
     4861 tattgtgccg agttaaagat ggcggaggca gtaccacgtt ctttcgtcgg gaaccaggcg
     4921 gcgacaatac gggcgttcgc cgggaaggat ggagcttccg agaatcccag cataaagcgc
     4981 ataaagaaca tggagatccc tgcccaagcc agcgggaaca tatcaacaaa gccttgcagg
     5041 aaggtgaaca gcgaccagaa aaagaggctg taggtgtaaa cttttttcga gccaaactta
     5101 tcaagcaacc agccgccggg gatttgcatc agcaagtagg cccagccaaa agcggagaag
     5161 atgtaaccca tcgaaaccgc acttaactgc aactcttttg ccacttcggt accagcaata
     5221 gacagcgttg cacgatcggc gtagttaacg gcggtaacaa taaaaataat cagtaatatt
     5281 aaatagcggg tatgcacacc tttccttttt tcgtcaacgg tgtccagaat cattttattt
     5341 acctcgggta cttatgctga tttttattat tatggggaag gtgttattta taagtttcat
     5401 ttatgccgta acgacaatga actcgggaat tagtataagc agcgcgagaa taataatcat
     5461 tgtgcaaatg ctaatttaat taatactttt taaatattat tttgagcata tgcacataag
     5521 gttgcgcgct aaagcacaga tttgcgcttt accttgccgg gcggcactgc aatccctaaa
     5581 atgattgaca ttgatcacat ttctgcgttt aaactcctga cattcttatt tcacccaatg
     5641 aaatcattta tttttaaatg agaccaggtc ctcattttaa taacccctgg ctggagaata
     5701 ttgcacaatg gccaacatcg aaatcagaca agaaacgcca actgcgtttt atataaaagt
     5761 tcacgacaca gataatgtgg caattattgt taatgataat ggcctgaaag caggaacgcg
     5821 ttttccggat gggctggaat taattgaaca tattccccag gggcataaag tcgcattgct
     5881 ggacattccg gctaatggtg aaattattcg ttatggcgaa gtgattggtt acgccgtgcg
     5941 tgcaatccca cgcggaagct ggatcgacga atcaatggtt gtactaccgg aagcgccgcc
     6001 gttacacacg ctgccactgg caaccaaagt cccggaaccc ttaccgccgc tggaaggata
     6061 cacctttgag ggctatcgca atgccgatgg cagcgtgggc accaaaaacc tgctcggcat
     6121 caccaccagc gtccactgtg tggcaggcgt ggtggactat gtagtaaaaa tcattgaacg
     6181 cgatctgcta ccgaaatacc cgaacgtcga tggcgtggtg gggctgaatc atttgtacgg
     6241 ttgtggcgtg gcgattaacg caccggcagc agttgtacct attcgtacca ttcacaatat
     6301 ttcgctgaac cctaactttg gcggcgaagt aatggtgatt ggcctgggtt gtgaaaagtt
     6361 gcagcctgag cgcctgctga ctggaacgga tgatgtgcaa gctattccag tagaaagcgc
     6421 cagcattgtc agtttgcagg atgaaaagca tgtcggtttt cagtccatgg tcgaggatat
     6481 tttgcaggtc gccgaacgcc atctacaaaa actgaatcaa cgtcagcgag aaacctgtcc
     6541 ggcttcagaa ctggttgtcg gcatgcattg cggtggcagc gatgcgtttt ctggcgtaac
     6601 ggcaaacccg gcggttggct atgcgtctga tctactggtg cgctgcggcg caacggtgat
     6661 gttttcagaa gtcacggaag tgcgtgacgc gatccatctg ctgacaccac gcgcagtgaa
     6721 cgaagaggtc ggcaaacggc tgctggagga gatggagtgg tacgataact atctcaatat
     6781 gggaaaaacc gaccgcagcg ccaacccttc gccgggcaac aagaaaggcg gtctagcaaa
     6841 cgtggtagag aaagcgctcg gctccattgc taaatcgggc aaaagtgcaa ttgttgaagt
     6901 gctgtcgccc ggtcaacgcc cgactaaacg cggattaatt tacgccgcga cgccagccag
     6961 cgattttgtc tgtggcacgc aacaggtggc ttcgggtatc accgtgcaag tgtttacgac
     7021 cggtcgtggt acgccgtacg gcctgatggc ggtacccgtc attaaaatgg caccccgcac
     7081 cgagctggcg aaccgctggt ttgatttaat ggatattaat gcgggcacca tcgctaccgg
     7141 cgaagaaact attgaagagg agggctggaa gttgttccac tttattctcg acgtcgccag
     7201 cgggaagaag aaaaccttct cggatcaatg gggactgcat aaccagctgg cggtgtttaa
     7261 cccagcaccg gtgacctgat aattcaattc gtattatgcc cgatagcaca cgtttatcgg
     7321 gcatattgag tcacagcgaa gacgcgtgat ccccgaccag aatcacttca accccagcct
     7381 ttcgcagtcc ttccagacta tccgcaggaa tgccttcatc aacaatgatc atgtcgatac
     7441 gttgagtatc aatgatctta tgtaaactgg aacgattgaa tttactggaa tcggtgacca
     7501 cgatgatccg ttccgcaact tcgcacatcc gacggcttaa acgggcttca tcttcattat
     7561 gtgtgctgac gccgcgctcc agatcgatcg catctacacc aagaaacagc atatcgaagt
     7621 ggtaattttg cagcgattgc tcagcctgat cgccgtaaaa agattgcgac tgacggcgca
     7681 aatgcccgcc ggtcatcagc agctcaacgc cttccgcttc cagcaacgca ttagccacgt
     7741 tcataccgtt ggtcatcgca attacgtcag tgtacttgcg catcagacga gcaatctcaa
     7801 aagtggtggt cccggaatcg aggatcaccc gatgacctgg ctgaatcaac tcaacggcag
     7861 ctttcgcaac gctgcgtttc atcgcggtgt tcagtgcgct tttatcttcc actgatggct
     7921 cgactgacgg cgtcgtgcta tcgcagatca acgcgccacc ataggcacgc acagcgatcc
     7981 cctgcttttc cagaaacgcc agatcattgc ggatcgtcac agtagatacg ccatacaatg
     8041 ccgacagatc gttaacctgc acactccctt gctgtcgcag acgctgaatg atctgttctc
     8101 gtcgctcgct ggtgcctgtc actcgcttct cacctgaagc gtcggtatta ctcatagtaa
     8161 gtcctttcgt aaaactttcg tttcatttcg ttttgcctat taacgccttt ctattaagca
     8221 aatgcaagcc caccttgccc attggcgcaa gctactctcg tttcagtgac tttcattacg
     8281 tttcttttgt gaatcagatc agaaaactat tatctttcgt tttattttta tctcaccatg
     8341 acgcagtatc aattgaaaca aaacgaaaga tcaatatcgc agtaatctga actggagagg
     8401 aaagtgaaac atctgacaga aatggtgaga cagcacaaag cgggcaaaac aaatgcaatt
     8461 tatgccgttt gttccgcaca tccgctggtg ctggaggctg caatccgcta cgccagtgca
     8521 aaccaaacgc cgttactgat tgaagcaacc tccaatcagg tagaccagtt cggcggttat
     8581 accggaatga cgcccgccga ttttcgcggc tttgtttgtc agctcgccga ctcgttgaat
     8641 ttcccgcagg atgcgctgat tctgggtggt gaccatctgg ggccaaaccg ctggcaaaac
     8701 ctgccagccg ctcaggcaat ggccaatgcc gatgatttga ttaaaagcta cgttgcggca
     8761 ggattcaaaa aaatccacct tgattgcagc atgtcctgtc aggacgatcc aattccctta
     8821 actgatgaca tcgtggctga acgcgccgcc cgtctggcga aagtggcgga agaaacctgt
     8881 cgcgaacact ttggcgaagc cgatctggag tatgtcattg gtaccgaagt gccggtacct
     8941 ggcggcgcgc atgaaacctt aagcgagctg gcggtcacca cgccggatgc cgcccgcgcc
     9001 acgctggaag cccatcgtca cgcctttgaa aagcaaggtt tgaatgccat ctggccacgc
     9061 attattgccc tggtggttca acccggcgtc gaattcgatc acaccaacgt tattgattat
     9121 cagcccgcca aagcgagcgc cttaagccag atggtcgaaa actacgaaac gctgattttc
     9181 gaagcgcact ctaccgatta tcaaacgccg caatctctgc gccagctggt gattgaccac
     9241 tttgccattc tgaaagttgg cccggcgctg accttcgccc tgcgtgaagc ccttttttct
     9301 ctggcggcga ttgaagaaga actggtacca gcgaaagcct gttccggtct gcgtcaggtg
     9361 ctggaaaacg tgatgctcga ccgcccggaa tactggcaaa gccactacca cggtgacggc
     9421 aacgcgcgtc gtctggcgcg tggttacagc tactcagatc gcgtgcgcta ttactggccg
     9481 gacagccaga ttgatgacgc tttcgctcat ctggttcgta atctggcgga ttcaccaatt
     9541 ccgctaccgc tcatcagcca gtatctgccg ctgcagtacg tgaaagttcg ctccggcgag
     9601 ctgcaaccga cgccacggga actcattata aaccatattc aggacatcct ggcgcagtac
     9661 cacacagcct gtgaaggcca ataagcaaaa caaagaggaa catgctatgc caaatattgt
     9721 tttaagccgg attgatgaac gcttgattca cggtcaggtc ggcgttcagt gggtcggatt
     9781 tgcgggggca aatctggtgc tggtagccaa cgatgaggtt gccgaagatc cggtacaaca
     9841 aaacctgatg gaaatggtac tggcagaagg gatcgccgta cgtttctgga cgctgcaaaa
     9901 agttatcgac aacattcatc gcgccgccga tcgacagaaa atcctgctgg tttgtaaaac
     9961 acccgccgat ttcctgacgc tggtgaaagg tggcgttccg gtgaatcgca ttaacgttgg
    10021 caatatgcac tacgccaatg gcaaacaaca aatcgccaaa acggtttctg tggatgcggg
    10081 cgatatcgca gcatttaacg acctgaaagc cgctggggtg gaatgcttcg ttcagggcgt
    10141 cccgacagag cctgctgtgg acctctttaa attactttga gggattcatc atggaaatca
    10201 gcctgttgca ggcatttgcg ttgggcatta tcgcctttat cgctggccta gatatgttta
    10261 acggcttaac ccatatgcac cgcccggtgg tcctcggccc gttggtcggg ctggtacttg
    10321 gcgatctgca taccggaatt ttaaccggcg atacgctgga actagtgtgg atggggctgg
    10381 ccccgctggc gggcgcacag ccgcctaacg tgattatcgg tactatcgtc ggcacggcgt
    10441 ttgccattac tactggcgtg aaacccgatg tcgcggtcgg tgtcgccgta cctttcgctg
    10501 tcgcagtaca gatggggatt accttcctgt tctcggtgat gtctggcgtg atgtctcgct
    10561 gcgaccggat ggcggagaac gccgatactc gcggcattga acgcgtgaac tatctggcac
    10621 tgctggcact cggcactttc tattttctct gcgctttcct gcctatctac ttcggcgcgg
    10681 aacatgcaaa aaccatcatt gatgtcctgc cgcaacgatt aatcgacggc ctcggcgtcg
    10741 cgggcggcat catgccagca atcggttttg ccgtgctgct gaaaatcatg atgaaaaacg
    10801 tctacatacc ctacttcatc ctgggctttg ttgccgcaac ctggctcaag ttaccggtgc
    10861 tggctattgc tgccgccgcg ctggcgatgg cgctgatcga tctgctgcgt aaatctcctg
    10921 aaccgactca acctgcggca cagaaagagg aatttgaaga tggcatctaa tcaaaccacc
    10981 ctgccgaacg tcagcgaaaa tgaagaaaca ctgctgactg gcgtcaatga aaacgtgtat
    11041 gaagatcgga gcattgccgc ggagctgacg aaaaaagata tcaaccgcgt cgcctggcgt
    11101 tccatgctgt tacaagcgtc ttttaactac gaacgtatgc aggcttctgg ctggttgtac
    11161 ggtctgctgc ctgctctgaa aaagatccac actaataaac gcgacctggc gcgcgccatg
    11221 aaggggcata tgggtttctt caatacccat ccgtttctgg tgacgtttgt tatcggcatt
    11281 atccttgcga tggagcgttc taagcaggac gttaacagta ttcagagcac caaaattgcc
    11341 gtcggtgcgc cgcttggcgg gattggcgat gcgatgttct ggctaacgct actgccgatt
    11401 tgtggcggga taggcgcaag cctcgcgcta caaggctcta ttcttggcgc agtcgtcttt
    11461 attgtgctgt tcaacgtggt acacttgggg ctgcgttttg gtctggcgca ttatgcttac
    11521 cgcatgggcg tggcggcgat tccccttatt aaagcgaata ccaaaaaagt cggccatgct
    11581 gcgtcaatcg ttggaatgac ggtaatcggc gcactggtgg caacctatgt tcgtttaagc
    11641 accacgctgg aaatcacggc gggcgacgca gtagttaagt tacaggctga tgttatcgac
    11701 aaactgatgc ctgccttctt accgctggtc tacaccctga ccatgttctg gctggtacgc
    11761 cgcggctaga gtccgctgcg cctgattgcg gttaccgtgg ttctagggat cgtcggtaaa
    11821 ttctgccatt tcctttaaat acaatgaggt attagatgtt aagtattatt ttgacagggc
    11881 atggcgggtt tgccagtggc atggaaaaag caatgaagca aatcctcggc gatcaatcgc
    11941 agtttattgc catcgatttt cccgaaacct caagcgccgc gctgcttaca tcgcagcttg
    12001 aagaggccat cgcccaactg gattgtgaag acggcattgt ttttctgacg gatttgctgg
    12061 gcggcacgcc gtttcgcgta gcttcaacgc tggcgatgca aaaaccgggc tgtgaagtga
    12121 ttaccggcac caatttgcaa ctgctactgg agatggtgct ggagcgcgaa gggttaagcg
    12181 gtgaagaatt ccgtgtacag gcgctggagt gtggacaccg agggctgacc agtctggtgg
    12241 acgagttagg ccgctgccat gaagaatgtc cggtcgagga aggaatatga cacacgttct
    12301 gcgcgccaga aggctgctga ctgaagaggg atggttcgat gaccatcagt tgcgtgttgc
    12361 tgacggtgtc atcgcagcaa tcgaaccgat tccagtgggc gtgactgaac gcgatgcgga
    12421 acggctctgc cccgcttaca tcgacaccca tgtacacggt ggtgcgggcg ttgatgtaat
    12481 ggatgacgcg ccggatgtac tcgacaagct ggcaatgcac aaggcacgcg aaggtgtcgg
    12541 cagttggtta ccgaccaccg taaccgcgcc gcttaatacc attcatgcgg cgctgaaacg
    12601 tattgctcaa cgttgccaac gcggcggacc tggtgcgcaa gtgctgggga gttatctcga
    12661 aggaccgtac ttcacgccgc agaataaagg cgcgcatccg
//
LOCUS       DAZOYF010000112        12566 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZOYF010000112 DAZOYF010000000
VERSION     DAZOYF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.112"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            30..1544
                     /gene="caiT"
                     /locus_tag="ACHUOF_002951"
     CDS             30..1544
                     /gene="caiT"
                     /locus_tag="ACHUOF_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR0612029.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1575..2717
                     /gene="caiA"
                     /locus_tag="ACHUOF_002952"
     CDS             1575..2717
                     /gene="caiA"
                     /locus_tag="ACHUOF_002952"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR0612030.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2846..4063
                     /gene="caiB"
                     /locus_tag="ACHUOF_002953"
     CDS             2846..4063
                     /gene="caiB"
                     /locus_tag="ACHUOF_002953"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFR0612031.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4137..5690
                     /gene="caiC"
                     /locus_tag="ACHUOF_002954"
     CDS             4137..5690
                     /gene="caiC"
                     /locus_tag="ACHUOF_002954"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR0612032.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5799..6584
                     /gene="caiD"
                     /locus_tag="ACHUOF_002955"
     CDS             5799..6584
                     /gene="caiD"
                     /locus_tag="ACHUOF_002955"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR0612033.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6590..7180
                     /gene="caiE"
                     /locus_tag="ACHUOF_002956"
     CDS             6590..7180
                     /gene="caiE"
                     /locus_tag="ACHUOF_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR0612034.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7266..7661)
                     /gene="caiF"
                     /locus_tag="ACHUOF_002957"
     CDS             complement(7266..7661)
                     /gene="caiF"
                     /locus_tag="ACHUOF_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR0612035.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7923..11144)
                     /gene="carB"
                     /locus_tag="ACHUOF_002958"
     CDS             complement(7923..11144)
                     /gene="carB"
                     /locus_tag="ACHUOF_002958"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR0612036.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11162..12310)
                     /gene="carA"
                     /locus_tag="ACHUOF_002959"
     CDS             complement(11162..12310)
                     /gene="carA"
                     /locus_tag="ACHUOF_002959"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR0612037.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
BASE COUNT         2939 a         3273 c         3324 g         3030 t
ORIGIN      
        1 aatattcgtt ttcacagtgg aattaactca tgaagaatga aaagagaaaa acgggaatag
       61 aaccgaaggt tttctttccg ccattaataa tcgtcggcat actttgttgg cttacagtca
      121 gagatctgga tgcggcgaat gtcgttatta atgctgtatt cagttacgtc accaatgtat
      181 ggggatgggc atttgaatgg tatatggtgg tgatgctttt cggttggttc tggctggtgt
      241 ttggcccgta tgccaaaaag cgtttaggta acgaaccgcc agaatttagc accgccagtt
      301 ggatctttat gatgttcgcc tcctgtacgt ctgctgccgt actgttctgg ggatcgattg
      361 agatctacta ctacatctcc accccgccgt ttggcttaga accgaactcg acaggagcga
      421 aagagttggg gctggcttac agcttgttcc actggggacc tctgccgtgg gccacttaca
      481 gcttcctttc agtcgccttc gcttacttct tctttgtccg caaaatggaa gtgattcgcc
      541 ccagctcgac actggtgccg ctggcaggtg aaaaacacgc caaagggttg ttcggtacca
      601 tcgtcgacaa cttctatctc gtcgccttga tcttcgcgat gggtaccagt ctgggccttg
      661 ccacgccgct ggtgaccgag tgtatgcaat ggttgtttgg cattccgcat accctgcaac
      721 tggacgctat catcattacc tgctggatta tcctcaacgc catttgcgtc gcttgcggtc
      781 tgcaaaaagg ggtacgtatc gccagtgacg tgcgtagtta cctgagcttc ctgatgctgg
      841 gttgggtgtt catcgtcagc ggtgccagct tcatcatgaa ctacttcacc gattcggtgg
      901 ggatgttgct gatgtatctg ccgcgcatgt tgttctatac cgatcccatc gctaaaggcg
      961 gcttcccgca gggctggacc gtgttctact gggcatggtg ggtgatttac gccatccaga
     1021 tgagtatctt cctcgcccgc atctcccgtg gtcgtactgt gcgtgaactg tgcttcggca
     1081 tggtgatggg gctgacagca tccacctgga tcctgtggac cgtgctcggt agtaacactc
     1141 tgctgttgat ggataaaaac atcatcaaca ttccaaatct gatcgaacag tacggtgtgg
     1201 cgcgcgccat catcgaaacc tgggccgctc tgccactcag caccgccacc atgtggggct
     1261 tcttcatcct ctgctttatt gccaccgtta cgctggttaa cgcctgctct tataccctgg
     1321 cgatgtctac ttgccgcgaa gtacgcgatg gtgaagaacc accgctgctg gtgcgtatcg
     1381 gttggtcaat tctggttggc attatcggta ttgttctgct ggcgctcggc ggcctgaaac
     1441 cgattcaaac cgccattatc gccggaggat gcccgctgtt cttcgtcaac attatggtga
     1501 cgctctcctt tattaaagac gcgaaacaga actggaaaga ttaattaacc cccaaaatat
     1561 caagaggttg aaagatggat tttaatttaa atgatgagca ggaactgttt gtcgccggta
     1621 tccgcgaact gatggccagc gaaaactggg aggcctattt tgccgagtgc gaccgtgaca
     1681 gcgtctaccc ggaacgtttt gtcaaagcac tggcggatat gggtatcgac agtctgctga
     1741 tccctgaaga gcacggtggt ctggacgcgg ggtttgttac tctcgccgcc gtgtggatgg
     1801 agctgggacg tctgggggca ccaacctatg tactgtacca gctgccgggc gggttcaata
     1861 ccttcctgcg cgaaggcaca caagagcaga tcgacaagat tatggctttc cgcggcaccg
     1921 gtaagcagat gtggaactca gcgattaccg aaccgggcgc gggctccgac gtgggtagcc
     1981 tgaaaacgac ttatacccgt agaaatggta agatttatct taatggtagt aagtgtttta
     2041 ttaccagtag cgcctacacc ccgtacatcg tggtgatggc gcgcgacggg gcttctccgg
     2101 acaaacctgt ctacaccgaa tggtttgttg atatgagcaa accgggcatc aaagtgacca
     2161 aacttgagaa gctcggtctg cgtatggata gctgctgtga aatcaccttt gacgatgtgg
     2221 aactggacga gaaagacatg ttcggtcggg aaggtaacgg ctttaaccgc gtcaaagaag
     2281 agttcgacca tgaacgtttc ctggtagccc tgaccaacta cggtacggcg atgtgcgcct
     2341 ttgaagatgc tgcgcgctac gccaaccagc gcgtgcagtt tggcgaggct attggtcgtt
     2401 tccagttgat tcaggaaaaa ttcgcccaca tggcgatcaa attaaactcc atgaaaaaca
     2461 tgctgtatga agcagcgtgg aaagcagaca acggcaccat cacctctggc gatgcagcga
     2521 tgtgcaaata cttctgcgcc aatgcggcat ttgaagtggt ggatagcgca atgcaggtgc
     2581 tgggcggtgt cgggattgcg ggcaaccacc gcatcagccg cttctggcgt gacctgcgtg
     2641 tagaccgcgt ttccggagga tctgacgaaa tgcagatcct gacgctgggt cgtgcggtgc
     2701 tgaagcaata ccgctaagtc cgtgcgctgc ctgatgcgac gctgacgcgt cttatcaggc
     2761 ctacgggagc acgaatgtaa gccggataag gcgtttacgc cgcatccggc agtcatgcgc
     2821 tacataaatt tttcaggagc taataatgga tcatctaccc atgccgaaat tcggaccgct
     2881 ggccggattg cgcgttgtct tctccggtat cgaaatcgcc ggaccgtttg ccggacaaat
     2941 gttcgcagaa tggggcgcgg aagttatttg gatcgagaac gtcgcctggg ccgacaccat
     3001 tcgcgttcaa ccgaactacc cgcagctctc ccgccgcaat ttgcacgcgc tgtcgttaaa
     3061 tattttcaaa gatgaaggcc gcgaagcgtt tctgaaatta atggaaacca ccgatatctt
     3121 catcgaagcc agtaaaggtc cggcctttgc ccgtcgtggc attaccgatg aagtactgtg
     3181 gcagcacaac ccgaaactgg ttatcgctca cctgtccggt tttggtcagt acggcaccga
     3241 ggagtacacc aatcttccgg cctataacac catcgcccag gccttcagtg gttacctgat
     3301 tcagaacggt gatgttgacc agccaatgcc tgccttcccg tataccgccg attacttttc
     3361 tggcctgacc gccaccacgg cggcgctggc agcactgcat aaagtgcgtg aaaccggtaa
     3421 aggcgaaagt atcgacatcg ccatgtatga agtgatgctg cgtatgggcc agtacttcat
     3481 gatggattac ttcaacggcg gcgaaatgtg cccgcgcatg agcaaaggta aagatcccta
     3541 ctacgccggt tgcggtctgt ataaatgtgc cgacggctac atcgtgatgg aactggtggg
     3601 cattacccaa attgaagagt gctttaaaga tattggcctc gcacatctac tcggtacgcc
     3661 ggaaatcccg gaaggcactc agcttatcca ccgtatcgaa tgcccttacg gcccactggt
     3721 agaagaaaaa ctcgatgcct ggctggcggc acataccatc gcagaagtta aagaacgctt
     3781 tgccgaactg aatatcgcct gtgccaaagt actgaccgta ccggaactgg aaagcaatcc
     3841 acagtatgtt gcccgtgaat caatcactca gtggcaaacg atggatggtc gcacctgcaa
     3901 agggccgaac atcatgccga aattcaaaaa taaccccgga caaatctggc gcggaatgcc
     3961 ctcacatggc atggacacgg ctgccatttt gaaaaatatc ggctacagcg aaaacgacat
     4021 tcaggagttg gtcagcaaag gtctggccaa agttgaggac taaacgcgtg gcgtcagtcg
     4081 gcttgcctga ctagcgcccg cccaataaca aacgaacaaa aatggataga ggtgcaatgg
     4141 atatcattgg cggacaacat ctacgtcaaa tgtgggacga tctggcggac gtttacggtc
     4201 ataaaacggc gctgatttgt gaatccagcg gcggagtcgt taaccggtat agttatcttg
     4261 agttaaatca ggagattaac cgcacggcaa acctgtttta tacgctgggg attcgcaaag
     4321 gcaacaaggt tgcactacat ctcgacaact gcccggaatt tatcttttgc tggttcgggc
     4381 tggcaaaaat tggcgcgatt atggtgccga ttaacgcccg cctgttacgc gaggaaagcg
     4441 agtggatctt gcaaaatagc caggcgtgcc tgctggtgac cagtgcgcaa ttttatccta
     4501 tgtatcaaca gattcagcag gaagatgcca ctcaattgcg gcacatttgc ctgacagatg
     4561 tggcacttcc cgctgatgat ggagtgagtt catttactca actgaaaaat caacaacctg
     4621 ccaccttgtg ctatgcaccg ccgctatcga ctgacgatac ggcggaaatt ctcttcacct
     4681 ccggcaccac ctcccgaccg aaaggtgtgg tgattaccca ttacaacctg cgcttcgctg
     4741 gatattactc cgcctggcag tgtgcactgc gtgacgatga cgtctacatg acggtaatgc
     4801 ctgcgtttca tatcgattgc cagtgtactg cggcgatggc ggcgttttct gccggggcca
     4861 cctttgtgct ggtcgagaaa tacagcgccc gcgccttctg gggacaggcg cagaagtacc
     4921 gcgccaccat taccgaatgt attccgatga tgatccgtac gttgatggtg cagccgcctt
     4981 cagcgaacga tcggcaacac cgcctgcggg aagtgatgtt ttatctcaac ttgtcggagc
     5041 aggaaaaaga cgcattttgt gaacgcttcg gcgttcgctt gctgacgtct tatgggatga
     5101 cggaaaccat tgtgggcatt atcggcgatc gccctagcga taaacgacgc tggccgtcga
     5161 ttggtcgggc ggggttttgc tacgaagcgg agatccgcga cgatcacaat cgcccgctcc
     5221 cggcaggtga gatcggtgaa atctgtatta aaggcgtacc agggaaaacc atcttcaaag
     5281 agtattttct caacccgaaa gccactgcga aagtgctgga agccgacggc tggctacata
     5341 ccggcgatac cggataccgc gacgaagaag gcttttttta tttcgtcgat cgccgctgca
     5401 atatgatcaa acgcggtggc gagaatgtct cctgcgtgga gctggaaaat atcatcgcca
     5461 cccatccgaa aattcaggac atcgtggttg tgggtattaa agattcgatt cgcgatgaag
     5521 ccatcaaagc atttgtggtg ctgaatgaag gtgaaacatt gagcgaagag gaattttttc
     5581 gcttctgcga acaaaatatg gcgaaattta aagtgccctc ttatctggag atcagaaaag
     5641 atctgccacg taattgctcg gggaaaataa ttagaaagaa tctgaaataa atgaaacgtc
     5701 agggaactac cctgccagct aataatcacg ccctaaaaca atatgcattt tttgcaggga
     5761 tgttgtcgtc cctaaaaaag caaaaatgga gaaaaggaat gagtgaatca ttacatctga
     5821 cccgcaatgg atcaattctg gaaattaccc ttgatcgccc aaaagcgaat gctattgatg
     5881 caaaaaccag ctttgaaatg ggcgaagtat ttctaaattt ccgtgacgat ccgcaattac
     5941 gtgtcgccat tattaccggt gccggagaga aattcttttc cgcgggctgg gatttaaaag
     6001 cggcagcaga aggcgaagca ccggatgctg actttggccc gggtggtttt gcgggattaa
     6061 ccgaaatttt caatctcgac aaaccagtta tcgcagctgt gaacggctac gcctttggcg
     6121 gcggtttcga actggcgctg gcggcagatt ttattgtttg tgccgataac gccagcttcg
     6181 ccctgccgga agccaaactg ggcatcgttc ctgacagcgg cggtgtgctg cgtctgccga
     6241 agatcctgcc gcctaccatc gtcaatgaaa tggtgatgac cggcagacga atgggcgcag
     6301 aagaggcgct gcgttggggg atagtcaacc gcgtggttag ccaggcggaa ctgatggata
     6361 acgcccgcga actggctcag cagctggtta acagcgcccc gctggcgatt gcggcgctga
     6421 aagagatcta ccgcaccacc agcgaaatgc cggtagaaga agcgtatcgc tatattcgca
     6481 gcggcgtgtt gaaacactat ccatcggttc tgcattcgga agatgccatt gaagggccgc
     6541 tggcgtttgc cgagaagcgc gatccggtgt ggaaaggacg ttaacaaccg tgagctatta
     6601 cgcctttgag gggttaattc cggtggttca cccgacggcg tttgtccatc ccagtgccgt
     6661 cttgattggc gatgtgattg tgggagccgg tgtctacatc ggcccactcg cctcactgcg
     6721 tggtgactac gggcggttga tcgtgcaagc gggagccaat attcaggatg gctgcattat
     6781 gcatggctac tgcgacactg acaccatcgt tggggaaaac ggccatatcg ggcatggagc
     6841 gatcctgcat ggttgtgtga ttggtcgcga tgcattggtc gggatgaaca gcgtaattat
     6901 ggatggcgcg gtcattggcg aagagagcat tgttgccgca atgagctttg tcaaagcagg
     6961 ctttagtggc gagaaacgcc agttgttgat gggtacgcca gcccgcgctg tacgcagtgt
     7021 tagtgacgac gagttacact ggaaacggtt gaataccaaa gagtatcagg atcttgtcgg
     7081 gcgctgtcat gcatcgttac atgaaacgca gccactgagg caaatggagg aaaatcgccc
     7141 ccgtttgcag gggacgacgg atgtgacgcc taaacggtga tcttgccgga tgcgacctgg
     7201 ttgctggata agatgctaca acatcgtatc tggcaagcag gacgcccggt atcaacgaga
     7261 taaaattaac gacgcatact ctttgacaac atcatctgcc acttctgctc gcggttaagt
     7321 tccgtgagcg gaacggcagg aatacgcggt gtctttctta cgccgactaa tttttcccga
     7381 ctgttattgc gcaggcgcgc gtaaatttgc tcatcgatat cgaccacttt aaccagtcgc
     7441 tgacactggc atccccgccc ttccagctta ttagggatca tcttaacttc gcagcttatt
     7501 tctgtgactt ccgacagaat ataagtcagg gtattaaccg ccttgctgtg ttcaatatcg
     7561 aaatgaatag agatctctct tgctatcacc caccgatttt cagccatcat ccattcggcg
     7621 attaacaagt agagtggttt ttcaacatat ccttcacaca tacgaactcc agtttcacaa
     7681 attaaaagtc tagctatgac tcgttttgaa gcaattaaac aaggccatat taaaattgtg
     7741 gatatatcga tagtatgcaa cttcaccgtc gataagtgtg agctaaatcc tgcttatgtc
     7801 atcaaaaata tcgattcccc aaaagattta cttaatgggc aaaaaaaaac ggtcctcatc
     7861 agagaaccgt attagttatt cgatcaaatt agcggatgaa aaatatccgc cacgacacgc
     7921 tattatttga tctgtgcgtg catttcctgc accgaaatta ctttttcagt cgcatcggca
     7981 ttcagcgcca tcgcggtagc gaaaccaccg ttcagggtgg tgtcataatg cactttatat
     8041 tgcagcgcac tgcgacggat cacgcgggag tcttcaatcg cacgacggcc tgaggtggtg
     8101 ttgatgatgt aggtatattc gccattcttg atacggtcct gaatgtgcgg acggccttca
     8161 tgcaccttgt ttaccagacg cggattgata cccgcttcgc ccagcacaat cgccgtgccg
     8221 tgggtcgcat ccagctcgaa gccctgtttc agcagttttg ccgccaggtc caccacgcgt
     8281 tctttatcgc cttcgcgcac ggaaagcagc gcacgaccgt gtttcttcat ggtggagttg
     8341 ctgcccagct gcgctttggc gaacgcttca gcgaaggtgc ggcccacgcc catgacttcc
     8401 ccggtagagc gcatttctgg ccctaacagc gggtcaacgc ctgggaattt attgaacggc
     8461 agcaccactt ctttcaccga gtagtacggc ggaataactt ctttggttac gccctgctca
     8521 gccagcgatt tgccagccat cacacgcgcc gccacttttg ccagcggtac gccggtggct
     8581 ttggagacga acggaacagt acgcgccgca cgcgggttta cttcaatcag gtaaacttcg
     8641 ttgtttttca ccgcaaactg cacgttcatc agaccgcgca cctgcaattc gaaggccagt
     8701 ttctgcacct gctggcgcat cacatcctga atttcctgac ttaaggtgta cgctggcagc
     8761 gaacatgcgg agtcaccgga gtgcacaccc gcctgctcga tgtgctccat gatgccgcca
     8821 atcagcacca tttcgccgtc gcagatggca tccacgtcaa cttctactgc gtcatcaagg
     8881 aaatgatcca gcaacactgg cgcatcgtta gacacgctaa ccgccgtctg gaagtagcga
     8941 cgcaggtcag cttcgtcata gacgatttcc atcgcccgac cgccgagaac gtaagacgga
     9001 cgcaccacca gcgggtagcc aatctctttt gccttctcaa ctgccatttc aatagcagta
     9061 acggtggcgt tcgccggttg tttcagtttc agacggtcaa ccgcatgctg gaagcgttca
     9121 cggtcttccg cacggtcgat agcatccggg ctggtgccga taaccggtac gccagcggct
     9181 tccagtgcac gcgccagttt cagcggggtc tgaccgccgt actgaacgat aacgcctttc
     9241 ggcttctcaa tacgcacgat ttccagcaca tcttccagag ttaccggctc gaagtagagg
     9301 cgatcggaag tgtcgtagtc ggtagagacg gtttccgggt tacagttaac cataatggtt
     9361 tcgtaaccgt cttcacgcag cgccagcgag gcgtgtacgc agcagtagtc gaattcgata
     9421 ccctgaccga tacggtttgg accgccgcca agcaccatga ttttttcacg gtcggtagac
     9481 ggattcgctt cgcactcttc ttcataagtg gagtacatgt aagcggtgtc ggtggcaaac
     9541 tctgccgcac aggtatccac gcgcttgtag accgggtgca ggtcatattg gtcacgcagc
     9601 ttacggattt ccgcttcgcg tacgcccgcc agttttgcca agcgcgcatc ggcaaagcct
     9661 ttgcgtttca gctggcgcag gaattcagcg ttcaggccag tgatgcccac ttccgccact
     9721 ttctcttcca gacgcaccag ttcttcaatc tgtaccagga accagcggtc aatgttagtc
     9781 aggttgaaga cgccgtccac agacaggccc gcgcggaaag catcggcgat gtaccagata
     9841 cgctctgcgc ctgcgtcttt cagttcgcga cggattttgg ttaacgcttc cgggtcatcc
     9901 aggctcactt tcgggtcgaa gccagttgcg cccacttcca ggccgcgcag cgctttttgc
     9961 agggattcct gctgcgtgcg accaatcgcc atcacttcgc caaccgattt catctgagtg
    10021 gtcagacggt cgttagcacc ggcgaatttt tcgaagttga agcgaggaat tttagtaacc
    10081 acatagtcga tggacggctc gaaggaggcc ggagtacgtc cgccagtgat gtcgttcatc
    10141 agttcgtcta gggtgtaacc caccgccagt ttcgccgcca ctttagcaat cgggaaaccg
    10201 gtcgctttcg acgccagcgc cgaagaacgg gacacgcgtg ggttcatttc gataacaatc
    10261 aggcgaccgt ttttcgggtt caccgcaaac tggacattgg aaccaccggt ttcaacgccg
    10321 atttcacgca gcaccgccat cgaggcgtta cgcatgattt gatattcttt gtcggtcagc
    10381 gtttgggctg gcgcgacagt gatggagtca ccggtgtgga tgcccatcgc atcaaagttt
    10441 tcgatagagc agacgatgat gcagttgtcg tttttatcac gcaccacttc catctcgtac
    10501 tctttccagc cgatcagcga ctcatcaatc agcaactctt tggtcggaga gagatccaga
    10561 ccgcgggcgc aaatttcttc aaactcttca cggttataag cgataccgcc gccgctaccg
    10621 cccatggtaa aggatgggcg aataatgcac gggaagccca cgtcagcggc aacagccagc
    10681 gcttcttcca tcgtgtgtgc gataccggaa cgcgcggttt ccagaccaat tttcttcatc
    10741 gctacgtcga aacgacggcg gtcttctgct ttatcaatcg catcggcagt ggcaccaatc
    10801 atggtgacac cgaactcttc caacacgccc tgacgttcca gctccagcgc gcagttcagc
    10861 gccgtctgac cgcccatggt tggcagcacc gcgtccgggc gctctttttc aataatcttg
    10921 cgtaccactt cccagtgaat cggctcgatg taggtcgcat cggccatttc cgggtcggtc
    10981 atgatggtcg ccgggttgga gttcaccaga atgacgcggt aaccctcttc gcgcagggct
    11041 ttacatgctt gcgcgccaga gtagtcaaac tcacacgcct gaccgataac aatcgggccc
    11101 gcacccagaa tcaggatact ttttatatct gtacgttttg gcatggctct tttactcctg
    11161 attacttagc ggttttacgg tactgctcaa ttaacttgat aaagtggtcg aacaacggcg
    11221 cggcgtcgtg tggaccaggg ctggcttcag ggtgaccctg gaagctgaac gccggcttat
    11281 cgatgcgatg aatgccctgt aacgtaccgt cgaacaggga tttatgcgtg acacgcaggt
    11341 ttgcaggtaa tgttgcttca tccaccgcaa aaccgtggtt ctgggcggtg atcatcacca
    11401 cgtttttctc cacatcttta accggatggt tgccgccgtg gtgaccaaat ttcattttga
    11461 cagtcttcgc accgctcgcc agcgccagca gctgatgacc gagacagatg ccgaataccg
    11521 gaatatcggt ttcgaggaac ttctggatgg cggtaatggc gtaatcgcac ggggccgggt
    11581 cgccaggacc gttggagagg aagatgccgt ctggattcat tttcagcaca tcttccgcag
    11641 aagtttgcgc cggaacgatg gtcaggcgac agcctctatc caccagcatc cgcaggatgt
    11701 tgcgcttggc accaaaatca taagccacga cgtggaacgg cagctcgtct tcttttttcg
    11761 cttctggcag gccgccggtc aacgtccagc tcccttgtgt ccagctatag gcttctgcgg
    11821 tggtcacttc ttttgccaga tccatgccgt tcagacctgg gaacgcgcgg gctttttcta
    11881 acgccagcgc cgcatccggg ttatcgcctg cgataatgca gccattctgt gcgcctttct
    11941 cgcgcagtaa acgcgtcagc ttacgggtat cgatatcggc aatcgccacg atgttatggc
    12001 gcttcaggta agaagagagg tcttcggtat tacggaagtt gctggcaatc agcggcaggt
    12061 cgcgaatcac cagaccttgt gcatgtacct gagaagattc ttcatcggcg tcattagtgc
    12121 cgacattgcc aatatgggga taagtaagag taacgatttg acgagaatag gaaggatcag
    12181 tgaggatttc ttgataaccg gtcattgaag tattgaaaac gacttcccca accgccgaac
    12241 ctgttgcccc tatggcccga ccgtgaaact gggttccgtc ttccagaacc aatagcgctg
    12301 acttaatcaa aacaccctcc agagaatact cactcacttt atttgcatat taattcacag
    12361 tgatgacgta aatcaatgca aatctgctta ccgacgaatt tctggcaaac ggcggcattc
    12421 tggagatatg ggcgctaaaa gtcaacttaa tggcaggatt ttttatatta ttttgtggct
    12481 catgcatgtt ttacaggcat taagcgacaa aaacatcatc taacctgttc tggaaaacgc
    12541 ttgcgcagca agaagattgc atgaaa
//
LOCUS       DAZOYF010000113        12305 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZOYF010000113 DAZOYF010000000
VERSION     DAZOYF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.113"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            758..841
                     /gene="dinQ"
                     /locus_tag="ACHUOF_002960"
     CDS             758..841
                     /gene="dinQ"
                     /locus_tag="ACHUOF_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR0612038.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHUOF_002961"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHUOF_002961"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR0612039.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHUOF_002962"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHUOF_002962"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR0612040.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="ACHUOF_002963"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="ACHUOF_002963"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR0612041.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHUOF_002964"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHUOF_002964"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR0612042.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7683)
                     /gene="dtpB"
                     /locus_tag="ACHUOF_002965"
                     /pseudo
     CDS             complement(6215..7683)
                     /gene="dtpB"
                     /locus_tag="ACHUOF_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            7849..7902
                     /locus_tag="ACHUOF_002966"
     CDS             7849..7902
                     /locus_tag="ACHUOF_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612043.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(8001..8435)
                     /gene="uspA"
                     /locus_tag="ACHUOF_002967"
     CDS             complement(8001..8435)
                     /gene="uspA"
                     /locus_tag="ACHUOF_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR0612044.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8829..9164
                     /gene="uspB"
                     /locus_tag="ACHUOF_002968"
     CDS             8829..9164
                     /gene="uspB"
                     /locus_tag="ACHUOF_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR0612045.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9235..10734)
                     /gene="pitA"
                     /locus_tag="ACHUOF_002969"
     CDS             complement(9235..10734)
                     /gene="pitA"
                     /locus_tag="ACHUOF_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR0612046.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10966..12165
                     /locus_tag="ACHUOF_002970"
     CDS             10966..12165
                     /locus_tag="ACHUOF_002970"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR0612047.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3006 a         3123 c         3304 g         2872 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgccagcaag aacgcggcag cctgcgtcag
     6421 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     6481 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     6541 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     6601 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     6661 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     6721 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     6781 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     6841 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     6901 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     6961 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     7021 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     7081 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     7141 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     7201 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     7261 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     7321 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     7381 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     7441 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     7501 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     7561 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     7621 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     7681 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     7741 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
     7801 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
     7861 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
     7921 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
     7981 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
     8041 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
     8101 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
     8161 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
     8221 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
     8281 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
     8341 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
     8401 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
     8461 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
     8521 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
     8581 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
     8641 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
     8701 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
     8761 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
     8821 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
     8881 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
     8941 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
     9001 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
     9061 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
     9121 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
     9181 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
     9241 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
     9301 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
     9361 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
     9421 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
     9481 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
     9541 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
     9601 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
     9661 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
     9721 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
     9781 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
     9841 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
     9901 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
     9961 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    10021 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    10081 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    10141 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    10201 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    10261 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    10321 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    10381 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    10441 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    10501 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    10561 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    10621 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    10681 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    10741 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    10801 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    10861 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    10921 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    10981 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    11041 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    11101 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    11161 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    11221 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    11281 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    11341 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    11401 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    11461 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    11521 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    11581 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    11641 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    11701 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    11761 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    11821 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    11881 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    11941 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    12001 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    12061 ggtgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    12121 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    12181 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    12241 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    12301 gtgta
//
LOCUS       DAZOYF010000114        12185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZOYF010000114 DAZOYF010000000
VERSION     DAZOYF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.114"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..602)
                     /locus_tag="ACHUOF_002971"
     CDS             complement(<1..602)
                     /locus_tag="ACHUOF_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR0612048.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            1122..2021
                     /locus_tag="ACHUOF_002972"
     CDS             1122..2021
                     /locus_tag="ACHUOF_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0612049.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            2070..3083
                     /gene="yhjD"
                     /locus_tag="ACHUOF_002973"
     CDS             2070..3083
                     /gene="yhjD"
                     /locus_tag="ACHUOF_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR0612050.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            3494..4816
                     /locus_tag="ACHUOF_002974"
     CDS             3494..4816
                     /locus_tag="ACHUOF_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0612051.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(4998..7058)
                     /locus_tag="ACHUOF_002975"
     CDS             complement(4998..7058)
                     /locus_tag="ACHUOF_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0612052.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(7128..7895)
                     /gene="pdeH"
                     /locus_tag="ACHUOF_002976"
     CDS             complement(7128..7895)
                     /gene="pdeH"
                     /locus_tag="ACHUOF_002976"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR0612053.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            8127..9056
                     /gene="kdgK"
                     /locus_tag="ACHUOF_002977"
     CDS             8127..9056
                     /gene="kdgK"
                     /locus_tag="ACHUOF_002977"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR0612054.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(9152..10648)
                     /locus_tag="ACHUOF_002978"
     CDS             complement(9152..10648)
                     /locus_tag="ACHUOF_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0612055.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(10869..12155)
                     /gene="dctA"
                     /locus_tag="ACHUOF_002979"
     CDS             complement(10869..12155)
                     /gene="dctA"
                     /locus_tag="ACHUOF_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR0612056.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
BASE COUNT         2920 a         3310 c         3000 g         2955 t
ORIGIN      
        1 caggaggaga tatttaacat cattgccgcc tgggtgcgat ttttcacttc cagacggcga
       61 tacagggatt ccagatgcgc ttttaccgtt ccggtactga tattcagcgc tctgccgatc
      121 tccttatttg attcgcccgc cgctaacatg gttaaaatct cccgctggcg ggcgcttaac
      181 gatttgagat ctttaatgtc cttttccggc gtcgtccgcc agtctccagg cagaaacatc
      241 atccccatcg ccgcactatt taccgccaac gcaaatgtct cgacggttga atcgcgaggc
      301 acaatggcca gcacattaaa atggataact tcctgtaacc accgtttatt gcaatccgtc
      361 gccgtgatta acaccttaac ctcaggaaac tgcaccacgg ttttttgcag caaccagtag
      421 caaaactcac catcctgatc gccatcgagc ataactaagg cttcagggta actttccagc
      481 ttttgccata actcgtctgc ctgactgacc ccctgaatac tcactcctgg aatacgctgc
      541 tgtaaactga tttttattcc atgaataaat attgactgcc tgtcaaacat gactatttgc
      601 ataactgaat ctccacctga atacgttaaa aagacttaag tagtggaagg gtattacccg
      661 cgagaaaaaa taagaattcg ccatttggcg gtggccattc tacagagatg acgcgtagaa
      721 aatagttacc gatataaata gttacagcta aacgcctgaa attacatgtc gagggcacta
      781 tttaaaacaa ttttgaggat ttccttatat tggtggttag tacgcatgca attaaaaatg
      841 aaattccgcg actacaagcc aaaataacaa atggcaatga gacaaaaata agcacaaata
      901 gccaatacgt cctctattca ctttaaaggg aatcactgaa aaatacgctc tgtttaaggg
      961 gattcacctt tctcataaag ctattccgcc cttttcctgc tgagaaatcg ccacattcgt
     1021 catgacaaca ttgtgaaacc cggcattaga tgttagaaaa aacaataaac aatgcgatat
     1081 gcgcgttact gtttcgctgt gaacaacaat cggtcaaaga aatggataaa attcacgcaa
     1141 tgcagttgtt catcaaagtc gcggagctgg aaagtttttc ccgcgcggcg gatttctttg
     1201 ctttgccaaa gggaagtgtt tcgcgccaga tacaggcact ggaacatcaa cttggcaccc
     1261 agcttctcca gcgcactacg cgacgggtca aactcacgcc agaaggcatg acctattatc
     1321 aacgggcaaa agatgtgttg agtaatctca gcgaactgga cggtctgttt caacaggatg
     1381 ccaccagtat cagcggtaaa ttacgcatcg acatcccgtc aggaatcgcg aaaaacctgt
     1441 tactgccgcg tctgtcggaa tttctctatc agcatccggg aattgagctg gaactgagta
     1501 gccataaccg tccggtagat attcttcatg atggttttga ttgcgtgata cgcactggcg
     1561 cgttaccgga agatggcgtt atcgcccgtc ccctcggcaa actaaccatg gtcaactgtg
     1621 ccagtccgca ctatctgacg cgctttggtt atccgcaaag ccccgatgat ctgacttcac
     1681 acgcaatagt gcgttataca ccgcacctgg gtgtacatcc gttaggtttt gaggttgcca
     1741 gcgttaatgg cgtccagtgg tttaagtctg gcggcatgtt gacggtaaac agtagcgaaa
     1801 actatctcgc cgccggtctt gccggtctgg ggattattca gatcccgcgc attgccgtgc
     1861 gcgaagccct gcgtgccggg cggcttattg aagtgttgcc aggctaccgt gccgagccgc
     1921 tctccctttc gctggtttac ccgcagcgtc gggagctttc ccggcgtgta aacctgttta
     1981 tgcagtggct ggctggcgta atgaaagagc acctggactg accgactata ctttttaaga
     2041 atgaccacga cagaaggaca aaagagcgga tgacgcagga aaacgagatc aaacgtccca
     2101 cccaggatct ggagcacgag ccgattaagc agttggataa tagcgagaaa ggcggcaaag
     2161 ttagccaggc gctggaaacc gtcaccacca ctgccgaaaa agtccaacgc caaccggtta
     2221 ttgcgcacct tattcgcgcg acagaacgct ttaacgatcg gctgggtaac cagtttggtg
     2281 cggctatcac ctatttctca tttttgtcga tgatcccgat tttgatggtg tcgtttgccg
     2341 cagggggctt tgtgctggcc tcccatccga tgttgctaca ggatatcttc gacaaaattc
     2401 tgcaaaacat cagcgatccg acactagccg ccacgttgaa aaacaccatc aacaccgccg
     2461 ttcagcagcg tacgactgta gggcttgtcg gcctggcggt ggcactttat tccggcatca
     2521 actggatggg taacctgcgt gaagcgattc gtgcccagtc gcgcgatgtc tgggaacgct
     2581 cgccgcaaga tcaggagaag ttctgggtaa aatatctgcg tgattttatt tcactgattg
     2641 gtttgttgat tgcgctgatt gtgacgcttt cgatcacctc ggttgccggt tcggcgcagc
     2701 aaatgattat tagcgccctg cacctgaaca gcattgagtg gctgaaaccg acgtggcgat
     2761 tgattggcct ggcgatttcc atcttcgcca actatctgct tttcttctgg atcttctggc
     2821 ggttgccgcg ccaccgtccg cgcaaaaaag ccctgattcg cggaacattt ctggctgcta
     2881 ttggttttga agtgattaaa atcgtgatga cctacaccct gccatcgctg atgaaatccc
     2941 cctctggcgc agcatttggt tccgtgctgg ggctgatggc atttttctac ttcttcgccc
     3001 gtttgacgct gttttgcgcg gcgtggattg ccaccgccga atataaagac gacccgcgaa
     3061 tgccagggaa aacgcagcct taaaataggc cggatgcggc gtaaacgcct tatccggcat
     3121 acgattttat tcggcctggc tccccgtagg ccggataaga tgcgccagca tcgcatccgg
     3181 ctataatgcg cacataacct cttgaaactc ttccccagag cctcttcagc catctatttg
     3241 ggagcaaaca atttcactcc aactcataac cccagcatat aaatccagtt ggtaactttt
     3301 atttaacctg aaaccagttt tatccactat ttataaaatt atgtgaagca tttcatagaa
     3361 gaaaaatcac tggcctaaac attatcccct ttttgcctgg tttttgacca tttccgcgat
     3421 ttgttacaca ttgaaatatc acttttgctg tgcgtaatat ggctattcgt tagccaaaaa
     3481 ataagaaaag attatgcaag caacagccac aacactcgac cacgaacaag aatacacgcc
     3541 gatcaactcg cgtaataaag tccttgtcgc ctctctcatt ggcacggcca ttgagttctt
     3601 cgacttttac atttacgcca ctgcggccgt tattgtgttt ccgcatatct tcttcccgca
     3661 gggcgatcca acggcagcaa cgctacagtc gctcgccacc ttcgccatcg ccttcgtcgc
     3721 gcgccccatt ggctctgccg tttttggtca ttttggcgat cgcgttgggc gtaaagcgac
     3781 gctggtcgcc tcgttgctaa cgatggggat ttcgaccgtg gtgattggtc tgctgccggg
     3841 ctatgccacg attggtattt tcgccccact gctgctggcg ctggctcgat ttggtcaggg
     3901 tctgggctta ggcggtgaat ggggcggcgc ggcgctgctg gcgactgaaa acgccccacc
     3961 gcgcaaacgt gcactgtatg gctccttccc acagctaggc gcaccgattg gcttcttctt
     4021 tgccaacggc actttcttgc tgctttcctg gctactgacc gatgagcagt ttatgagctg
     4081 gggctggcgt gtgccattta tcttctcggc ggtgctggtc attatcggcc tgtatgttcg
     4141 cgtgtcgctg catgagtcgc cggtgtttga gaaagtcgct aaagcgaaaa aacaggtgaa
     4201 gatcccgctg ggtacgctgc tgaccaaaca tgttcgcgta accgtactgg gtacgttcat
     4261 tatgctggca acctatacgc tgttttacat catgacggtc tactctatga ccttcagtac
     4321 cgccgccgcg ccagttgggc ttggcctgcc gcgtaacgaa gtgttgtgga tgctgatgat
     4381 ggcagttatt ggttttggcg tgatggtgcc agtcgccgga ttactggctg atgcctttgg
     4441 tcgccgtaaa agcatggtga tcatcaccac gctgatcatc ctgttcgcgc tgttcgcctt
     4501 taacccgctg ctcggttctg gcaacccgat tctggttttt gccttcctgc tgctggggtt
     4561 aagtctgatg gggctgacct tcgggccgat gggtgcgctg ttaccagagc tgtttccgac
     4621 agaagtgcgt tacaccggag catcgttctc ttacaacgta tcgtcgattc tcggggcttc
     4681 cgttgcgccg tatatcgcag cctggttgca ggctaactac gggttaggtg cggtggggtt
     4741 atatctggcg gcgatggctg gcttgacgtt aatcgccctg ctgctgaccc atgagacgcg
     4801 acatcagtcg ttgtaataag tgtgaaaagc ctgatgcgct acgcttatca ggactgaatt
     4861 tccatacaat atattgaatt cacaaggatt tgtaggccgg ataaggcgtt cacgccgcat
     4921 ccggcatgaa caaagcacac tttgccaaaa atctaaatct gtttaattgc cggatgtcag
     4981 acatccggca attatcatca cttcttcatc tgcgacaata tcgtccgaca ctgattagcc
     5041 tccccttcgg aaggtgagat cagtgccagt aacgccgccg ccggtgttac cagcgttgcc
     5101 agtgccgcag caacagcacc acgggcaatc agcggtccgg ctttcacccc agcctgcgga
     5161 tttttaaacg tcccacgcac atacagcggc gaacgcagtg tgataatccg aatcccttta
     5221 ctctccggat caatagtcaa atccagctgt tccgaagcaa aacttgccgt gccggtaaca
     5281 ttaatcaacg cgttctcagt atcgaaagca aaaatctgcg ggcgcgccac gccgttggca
     5341 atattcagat tcgccgccgc gcagttcacc cgcacctcat cgtcaccaaa tatcgcaccg
     5401 acaatgtagt tgccgacatt cagcccaaca atctccatca ggttgcggct caccagcccg
     5461 tcattcatca acagtttcag gttgccgtta ctgttgccta aaagcgccgc caccgagtta
     5521 ccgctaccgc gtagttccgc gtcaccgttc atttccccca gcgtcttctg catcagttcc
     5581 acatcgggca tcagttcttt cagtttcagt cgacgagcct gaatatctgc ccgcccctgc
     5641 atcggctttt tatcgccttc cagatgaata ttcgccgcaa tgctgccgcc cgccatgcca
     5701 aatttcagcg gttgcaggcg caggtcagca tttttgagga tgatatgagt agaaagatcg
     5761 ctaatcggca ggctactgcc atgctcaatg cgccgccctt tgaagcgaac atcggcatcc
     5821 ataacgtccc atttatcggt ttcgaagcgg tcataaggca gcactttgcc cgcaggctga
     5881 acgctttttt cgcccttctt ctgttcagac cgtttcgact tttctgcccc tttcccggaa
     5941 tcaacgccaa tcaacggtcc caggtccgcc agccgcaatt gccgcgactc gacatcacct
     6001 tccagttttg gtcgtggctt tccggtggtg tagaccagag aaccgtggat atcgctatcg
     6061 ccaattcgcc cattaaaacc gcgataatca aagaccgacg atttttcagt gtcgattttc
     6121 gctaccagcc gaccatccgt ttcaaacggc ggggtatcgg gcagcagaac gcccgtcagt
     6181 tcatagagat cacccagtga atcgccagaa aatttaagcc gtaaatcgac accgcccatc
     6241 ttcattgggt cattcacgac gccatcaaaa gcaacacggg tattacctga acggaaatca
     6301 gcctgtaccg gaaacggcgt cccttcgcca cgcagcgcca gcataccgcc tatttttccc
     6361 gtacccgtga gcggttcacc gttataacgt ccctgcgcct tcaggccaaa aatgtaatcg
     6421 cccacctttt ctttatccgc tttacctttc gatccagtaa cttcgctgaa tggcagcggc
     6481 ttgcctaacg gatcaacaaa aatttccaga tccgctttgc ttactttgtc atcaatggcg
     6541 atccgccctt gatcgaaaag aatattatcc agccgaaacg accatgccga cggctttgca
     6601 ttcgcgtctt tgttgtcatc gttggcaaga ttaaacgtcc agttattgtt cttttcagag
     6661 aggcgaatca ggcgcgcgtc gggcttttcg agcttgatcc acggcagcca gaccgttttg
     6721 gtcagcagcg ccagcggtgc cagcgttgcc tctactcgcg gcaaatgcac catcgtgact
     6781 tcgggaatat ccggtggatt accaagaatg atgtcttctg catgtacatg aggccacggc
     6841 acccagctgc gccagccagt ttcttgtttt tgccgctccc acaccacgcc cagattgcca
     6901 cggatagcga acggacgatt caactccgca gagactttct ggttgatggt cggtttgagt
     6961 cgattccagt caaaagttgc aatcaaaatg atcgccacga caatcaacaa caagaaagcc
     7021 cctgaaatcg cagcggttat tttgcctgcc ttgctcatcg ttcgcttcct catattcttc
     7081 ctgtgccagt cctaaagata gtccagcccg gcggaaaatg aggcagctta tagcgccaga
     7141 accgccgtat tcagcgtttc catcggtgcc gggcgtgaaa gaaaccagcc ttgtgcggcg
     7201 aatgcgggcg agttctgaac atcacgccac tcttccggtg tttctacgcc ctcgacaatc
     7261 accccgcgac aatagcgatt cattagatgt aaaagctgag aaaagagtgt gcggccttcc
     7321 ggcgactgac gcagcatcac aaacagttct cgcgcgattt tgatgtagtc ataacgcact
     7381 tcacttagtg cagagaaatt tgccatcccg gtaccaaaat catccagcca cagcgggcca
     7441 aattcacaca tcgaggcaaa ggttgaatct ttcggcagac ggatatgctc caccagttcg
     7501 aaacgcagcc agggaagacg ctcaatctgg cgcaggattt ttggttgctg acgcagggcg
     7561 atgagcgtag ggccatcaat attgaccgat gccagcaggc cgtgctctac aaagaagtcg
     7621 gctttttgcg ccagcaaatc aatctgctct ttcacaacct ccatccgatg gctgacggtg
     7681 atttcagtaa aatagcgatc cggcggcagg cgttgcgaag ggttcaaggg atgcgtgacc
     7741 accgttaata gctccacggc cattaaccgc ccgcatgttt gatagatcgg ctgccaggtg
     7801 taagcacgct cacactgcaa ccaaaaacgc cgttcctgca agctctcgat gcttgcttca
     7861 gggttgctta ttcgctggat aacctgcctt atcatcaaag atgtcctgtg tttaaaagtg
     7921 atgctgcccg gactcgacaa agattatcgg cgcgtaaggg cagaacttta ccgcctggcc
     7981 ggggtaaatt tcctcactcc acaacggaat ctttcaggaa tacgttctgg ctcaaaaaaa
     8041 taatggaaca ctgttttaat atggttgacc agcaaaccac cacagcgcaa actaacgcta
     8101 attttttaca gatcaggttc acgactatgt ccaaaaagat tgccgtgatt ggcgaatgca
     8161 tgattgagct ttccgagaaa ggcgcggacg ttaagcgcgg tttcggcggc gataccctga
     8221 acacttccgt ctatatcgcc cgtcaggtcg atcctgcggc attaaccgtt cattacgtaa
     8281 cggcgctggg aacggacagt tttagccagc agatgctgga cgcctggcac ggcgagaacg
     8341 ttgatacttc cctgacccaa cggatggaaa accgtcttcc gggcctttac tacattgaaa
     8401 ccgacagcac cggcgaacgt acgttctact actggcgtaa cgaagccgcc gccaaattct
     8461 ggctggcgag cgagcagtct gcggcgattt gcgaagagct ggcgaatttc gattatctct
     8521 acctgagcgg gattagcctg gcgatcttaa gcccgaccag ccgcgaaaag ctgctttccc
     8581 tgctgcgcga atgccgcgcc aaaggcggaa aagtgatttt cgacaataac tatcgtccgc
     8641 gcctgtgggc cagcaaagaa gagacacagc aggtgtacca gcaaatgctg gaatgcacgg
     8701 atatcgcctt cctgacgctg gacgacgaag acgcgctgtg gggtcaacag ccggtggaag
     8761 acgtcattgc gcgcacccat aacgcgggcg tgaaagaagt ggtggtgaaa cgcggggcgg
     8821 attcttgcct ggtgtccatt gctggtgaag cgttagttga tgttccggcg gtgaaactgc
     8881 cgaaagaaaa agtgatcgat accaccgcag ctggcgactc tttcagcgcc ggttatctgg
     8941 cggtacgttt gacgggcggc agtgcggaaa acgcggcgaa acgcgggcac ctgaccgcaa
     9001 gtaccgttat tcagtatcgc ggcgcgatta tcccgcgtga ggcgatgcca gcgtaaacgc
     9061 agcatatgca aaaggctgga taaagtgtta cccctctatc cagctcatcc ataaatacct
     9121 gatggttgtg ctaactcatc accatcaggc attactgtgc aggtggaata tccgtcactt
     9181 caggatgtac gtcatccgtg gcgacagagg tggtcgcagc ggcggtagat ggggccatga
     9241 tttgatccca ggccgcctgc aacgctttca tgttaaattc cggctcgcct ttcggctgca
     9301 gcagtattaa cgccatatca ttcgacaact gctgacgcag atcctgattt aacatctcca
     9361 cagtcaggct gttaaggaaa tcctgccgca gtttctgata ctgctccggc gcgatatcga
     9421 cgacctgatt ttgcaacgaa cgcatccgct ggcccatcag aatatcggta tcagcccgtg
     9481 cataggcggc aaacagtttc tgcagctcca gtttcttttg cgccactaac gcattgaact
     9541 cttcttccgg cagaccttta tcgcgaacct tcgccagttc acgcgccacc agattaaggt
     9601 tgctgttcag cttgtcgttt ggtgattcga tgttgatggc acactgcgca cgcagataca
     9661 gcacacggca gtcaaatcca agaccgatgt ctttgctgtc actggcgctt aacgcttgct
     9721 gaacatgcca gaacagcgcc tcgcgggcca ggtccgcacg ccagtagcgc agcagtgcgg
     9781 ctgattcacg aatcggctgc cacggcgtat cccacatgat agataaccgg tcctgacgca
     9841 ccgcgtcagt cataatactc accgcttccg cacgcagcgg agaaagcgtc ggcaccggag
     9901 ctggcgtttc acgtttgcct ttcagttcgc caaacgtttt gttgatttgg tcgacaaccg
     9961 agcgcgcatc cacgtttccc accaccagta gcgtcattgc atccggggtg taccatttct
    10021 gatagaaatc tttaattttt tccgcttcaa cgggttgttt cagcggatcg gcaggatcgt
    10081 gacctaacaa ggttgaccct ttcagacgat agcgccacca gccctcttta gtatcggcag
    10141 gccaggttgc caccatgtcc tgactttgca gcgcgtggtt gatggtttct ggtgtgatgg
    10201 tcaatttgcc agtggcattt gccagataag agagcgcttc tttcagcaaa tcgttacggt
    10261 tattgggcaa actcagatta aacagcgtgg tgtcataaga gacaattacc ggcggcatcg
    10321 ggcgtttagg gtcgatcccc tgctgccaca atgaacgcgc ctgtgctgcg tcaaggccac
    10381 cgctttgcgt tagcgcaata cgagggatgg cgtgactgta accgctctgt tgtgtacttt
    10441 cggcgagcga accggtatta accagcaggc gaatttcaac acgatcgctg ggacgctggg
    10501 gggtggtcag cacttgccac tgtaaaccgt tggaaagcgt cccctgttgc catgctggat
    10561 caggctggag cgcatctgcc tgcacatagc cagcagtggc catcatcagc aaaccgcccg
    10621 ctaaaagtcg aatttttatg ccctgcatgt gaacccctga tcaactatcc tggtaataaa
    10681 aaaactgccc gttgtcggtc agtctaaatg acgttaaaaa cacttcgtgt tagaccgcaa
    10741 gaacatgaaa atgtcacgga agaagtgaaa taaacctgaa ctcacccagg tggtaagttc
    10801 aggcacaggg gtcaattatg cgcaaacacc cgcactcggg gaagggagtg cgggcataag
    10861 tggtgagatt aagaggataa ttcgtgcgtt ttgccatccg gcgcacgatt attcagcaca
    10921 tcgtccagtt ttttgtggtc cagttctttc acccacttag caacgacaat ggtcgctacg
    10981 ccgttaccga ccaggttagt tagcgcacga gcttctgaca taaagcggtc gataccgagg
    11041 atcagcgcca gacccgctac cggcaaatgg cccaccgcag agagcgtcgc cgccagcacg
    11101 ataaagccac tacccgttac ccctgccgcc cctttagaag aaagcagcaa cacgattaac
    11161 agcgtgattt ggtggacgat atccatctga ctgttagtgg cctgggcgat aaacaccgcc
    11221 gccattgtca ggtatatcga tgtgccatca aggttaaacg agtagcctgt cgggatgacc
    11281 agccccacca ccgatttacg gcagccgagt ttctccatct tgtcgagcat acgcggcagc
    11341 gccgactcgg aagatgaagt ccccagtaca atcagcagtt cttcacggat gtagcggata
    11401 aatttgaaga tactgaaacc agtcgcttta gcgattgaac ccaataccag caccacaaac
    11461 aggatacagg taatgtagaa acagataatc agctgcccca gttgcaccag tgtgccgacg
    11521 ccgtatttac cgatggtaaa cgccattgcc ccgagcgcac caataggtgc cagacgcatg
    11581 atcatattga tgatgccgaa gatgacctgc gagaaacttt cgatcacgtt aaaaatcagt
    11641 tggcctttgc tgcccagacg gtggagcgca aaaccaaaca gtacggcaaa cagcagcacc
    11701 tgcagaatgt taccgctggc aaatgcgcca atgacgctcg ccgggatgac atccataatg
    11761 aaggcgacaa tgccctggtc tttcgcctga tcggcgtaaa ccgctaccgc tttcgcatca
    11821 agcgttgccg gatcgacgtt cattccggca ccaggctgca cgacgttaac gatgataaga
    11881 ccaataatca gcgcgatggt actgacaatt tcaaagtaaa gcagtgcgac tgcgccggta
    11941 cgaccgaccg ccttcatgct ttccatgccc gcaatgcccg ttacgacggt acaaaagatg
    12001 acaggagcga tgatcatctt aatgagctta acgaagccgt cgccaagcgg tttcatttgc
    12061 tcgcctattt caggatagaa atggccaagg agaataccaa tggctatcgc tgtcaggacc
    12121 tgaaagtaaa ggcttttaaa cagagaggtt ttcatagggt gtcctttagt aaaaccacag
    12181 gtctt
//
LOCUS       DAZOYF010000115        12153 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZOYF010000115 DAZOYF010000000
VERSION     DAZOYF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.115"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            126..995
                     /locus_tag="ACHUOF_002980"
     CDS             126..995
                     /locus_tag="ACHUOF_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR0612057.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="ACHUOF_002981"
     CDS             complement(1198..1551)
                     /locus_tag="ACHUOF_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR0612058.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHUOF_002982"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHUOF_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR0612059.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="ACHUOF_002983"
     CDS             complement(3379..3672)
                     /locus_tag="ACHUOF_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR0612060.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHUOF_002984"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHUOF_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR0612061.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /locus_tag="ACHUOF_002985"
     CDS             complement(5220..5696)
                     /locus_tag="ACHUOF_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR0612062.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="ACHUOF_002986"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="ACHUOF_002986"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR0612063.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHUOF_002987"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHUOF_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR0612064.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="ACHUOF_002988"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="ACHUOF_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR0612065.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHUOF_002989"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHUOF_002989"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR0612066.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="ACHUOF_002990"
     CDS             11052..11627
                     /locus_tag="ACHUOF_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0612067.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="ACHUOF_002991"
     tRNA            11734..11809
                     /locus_tag="ACHUOF_002991"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2932 a         3140 c         2979 g         3102 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgt gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa caa
//
LOCUS       DAZOYF010000116        12117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZOYF010000116 DAZOYF010000000
VERSION     DAZOYF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12117
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.116"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            228..998
                     /locus_tag="ACHUOF_002992"
     CDS             228..998
                     /locus_tag="ACHUOF_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR0612068.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            989..1462
                     /locus_tag="ACHUOF_002993"
     CDS             989..1462
                     /locus_tag="ACHUOF_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR0612069.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1569..2108
                     /gene="dnaT"
                     /locus_tag="ACHUOF_002994"
     CDS             1569..2108
                     /gene="dnaT"
                     /locus_tag="ACHUOF_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR0612070.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2111..2848
                     /gene="dnaC"
                     /locus_tag="ACHUOF_002995"
     CDS             2111..2848
                     /gene="dnaC"
                     /locus_tag="ACHUOF_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR0612071.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2897..3391
                     /gene="yjjA"
                     /locus_tag="ACHUOF_002996"
     CDS             2897..3391
                     /gene="yjjA"
                     /locus_tag="ACHUOF_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR0612072.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3631..5922
                     /gene="opgB"
                     /locus_tag="ACHUOF_002997"
     CDS             3631..5922
                     /gene="opgB"
                     /locus_tag="ACHUOF_002997"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR0612073.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6060..7082)
                     /gene="lgoD"
                     /locus_tag="ACHUOF_002998"
                     /pseudo
     CDS             complement(6060..7082)
                     /gene="lgoD"
                     /locus_tag="ACHUOF_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7221..8135
                     /locus_tag="ACHUOF_002999"
     CDS             7221..8135
                     /locus_tag="ACHUOF_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR0612074.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8350..9711
                     /locus_tag="ACHUOF_003000"
     CDS             8350..9711
                     /locus_tag="ACHUOF_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0612075.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9760..11424)
                     /gene="tsr"
                     /locus_tag="ACHUOF_003001"
     CDS             complement(9760..11424)
                     /gene="tsr"
                     /locus_tag="ACHUOF_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFR0612076.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11543..11989)
                     /gene="hpaR"
                     /locus_tag="ACHUOF_003002"
     CDS             complement(11543..11989)
                     /gene="hpaR"
                     /locus_tag="ACHUOF_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR0612077.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2960 a         3194 c         2932 g         3031 t
ORIGIN      
        1 ctgacccgta aaggctaaac aataaattca ttatagtgat aatattcatg tgatagtttg
       61 tttgcggcga gagataattc gctttttatc accgagcttg tcccaataac caggattaat
      121 cagcgcaatt ttgatcttaa cctaccgcaa aacaaccgtg cttcatgata caaatggatt
      181 ctcattatct gaacaatacg cagcacgtct atgacaaagg gcgagttatg caaactgagc
      241 aacagcgagc cgtaacgcgg ctttgtatcc agtgtggatt atttcttttg caacatggtg
      301 cggaaagcgc gttggttgat gagctttcct cacgactggg tcgggcgctg ggaatggaca
      361 gcgtcgaaag ttctatctct tcgaacgcca tagtgctgac aactattaaa gatgggcaat
      421 gcctgacatc gacacgtaaa aatcacgatc gcggcattaa tatgcatgtg gtgactgaag
      481 tccagcacat tgtgattctt gcggagcatc atctgctgga ttacaaaggc gtagagaaac
      541 gatttagcca aattcaacca ttacgttacc caagatggct ggtagcctta atggttggcc
      601 tttcttgcgc ctgtttctgt aaactcaata acggtggctg ggatggtgcc gtcatcacct
      661 tctttgccag tacgaccgcg atgtatatcc gccagctgct ggcacaacgt catcttcatc
      721 cacagatcaa cttttgcctt accgctttcg ccgccaccac catttccgga ttgcttttgc
      781 aactccccac tttcagcaat acccccacca ttgcgatggc cgccagcgtt ctgctgctag
      841 tgccgggctt tccgttgatt aatgccgtcg ccgatatgtt taaaggccac atcaataccg
      901 gactggcacg ctgggcgatc gccagtctgc tgacactggc tacctgcgtc ggcgtagtga
      961 tggcactgac gatttggggg ctacgcggat gggtgtgatc gaatttctgt tagcgttggc
     1021 gcaggatatg atcctcgccg ccattcctgc ggtcggcttt gcgatggtgt tcaacgttcc
     1081 cgtgcgggcg ttacgctggt gtgcgctgct tggctcgata ggtcatggtt cccgaatgat
     1141 cttgatgacc agcgggttga atattgagtg gtcaaccttt atggcttcta tgctggtcgg
     1201 taccattggt attcaatggt cgcgctggta tctggcgcat ccgaaagtgt ttaccgtggc
     1261 ggccgttatc cctatgttcc cgggcatatc ggcttatacc gcaatgattt cgtcggtaaa
     1321 aatcagccag ttaggttaca gcgaaccgtt gatgattacc ctgttaacca actttcttac
     1381 agcttcatcg attgttggtg cgttatccat cggtctttcc attcctggat tatggttgta
     1441 ccgcaagcgc cctcgcgtat aaaattgcct cgcttaagca cacggatgag agacagcctc
     1501 ctctcctccg tgtgttacta taaaagttat ctcccttctc gttcatcgtt ccatatttga
     1561 gaaacagtat gtcttccaga gttttgaccc cggacgtcgt tggtattgac gccctggtac
     1621 acgatcacca aaccgttctg gcaaaagctg aaggcggtgt ggttgccgta tttgctaaca
     1681 atgccccggc gttttatgcc gtcacgcctg cacgcctggc tgaactgctg gcgctggaag
     1741 aaaagctagc gcgtccggga agcgatgtcg ctctggacga tcaactctat caggaaccgc
     1801 aagccgctcc cgttgctgta cccatgggga aattcgccat gtatccggac tggcaacccg
     1861 atgccgattt tatccgcctg gcggcgctat ggggcgtggc gctaagagag ccggtgacca
     1921 ccgaagaact ggcctcattc attgcctact ggcaggcgga aggtaaagtc tttcaccatg
     1981 tgcagtggca acaaaaactg gcgcgcagcc tgcaaatcgg tcgtgccagc aacggcggac
     2041 tgccgaaacg agatgtgaat acggtcagtg aacctgacag ccaaattcca ccaggattca
     2101 gagggtaacg atgaaaaacg ttggcgacct gatgcaacgc ctgcaaaaaa tgatgcctgc
     2161 ccatatcaaa cctgcattca aaacgggtga agagctactg gcctggcaga aagaacaagg
     2221 ggcgatccgc tccgccgctc tcgaacgtga aaatcgggcg atgaaaatgc agcgcacctt
     2281 taaccgctcc ggtattcgtc cactgcatca gaactgctcc tttgagaact atcgcgttga
     2341 gtgtgaaggg cagatgaatg cgttaagcaa agcgcgccag tatgtcgaag agtttgacgg
     2401 caacatcgcc agcttcatct tttctggtaa gccaggaacc ggcaaaaacc atctggcggc
     2461 ggcaatctgc aacgagctgc tgctacgcgg taaatccgta ttgatcatca ccgtggccga
     2521 tattatgtcg gcgatgaaag ataccttcag gaatagcggt accagcgaag aacaactgct
     2581 taacgatctg agcaacgttg atctgctggt gatcgatgag atcggcgtgc agaccgaatc
     2641 gaaatacgaa aaagtgatca tcaaccagat cgtcgatcgc cgctcttctt ccaaacgccc
     2701 aaccgggatg ctgaccaaca gtaatatgga agagatgacc aagttgctgg gcgaacgcgt
     2761 gatggaccgt atgcgcctgg gtaacagttt atgggtgatc ttcaactggg atagctaccg
     2821 tagccgggta acgggtaaag agtattaatc cccgtttctg gcggtatact gcgacacaat
     2881 tccaccttga gtaatgatga tgaaaactat taaacatctt ctgtgctgtg ccattgccgc
     2941 cagcgcatta atttccaccg gggtgcatgc tgcgtcctgg aaagatgcgc tctccagcgc
     3001 cgccagcgaa cttggcaacc aaaacagcac gacacaggaa ggcggttggt cgctcgcgtc
     3061 attaactaac ttgcttagca gcggaaacca agccttaagc gcagataaca tgaacaatgc
     3121 cgcaggcatt ctgcaatact gcgcgaagca aaagctggct tcggtaaccg atgccgaaaa
     3181 catcaagaac caggtgctgg aaaagctggg cctgaacagt gaagagcaaa aagaagacac
     3241 caactatctg gacggtattc agggtttgct gaaaacaaaa gatggtcagc aactcaatct
     3301 ggataacatc ggaacgactc cgctggcaga aaaggtgaaa accaaagcct gcgatctggt
     3361 gttaaagcag gggctgaact tcatttcctg aaccgcgaaa tgttacccgg ctgacgcggc
     3421 tctctcctgc ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc
     3481 gcgatcaaaa aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta
     3541 gaattgcagc aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc
     3601 taaacggccc ggtttagtga ggatcgccca ttgtcagaac tactctcttt cgcccttttt
     3661 ctcgcctctg tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc
     3721 acgttaacgg tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat
     3781 tttactggcg atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt
     3841 gctggcgtca gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg
     3901 ttcggtgcgc tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac
     3961 agcctgctgg cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag
     4021 ataacggaac tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat
     4081 aaagagccgt cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa
     4141 agtctcgagc ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc
     4201 gcgttgaaaa atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac
     4261 acgattgcgg gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc
     4321 aacgcctccg cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg
     4381 aaaaactcgg gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa
     4441 gatgtgttcc tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc
     4501 gtggtagccg acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat
     4561 gaagcgtgga aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc
     4621 ctgacagtcg atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa
     4681 tatgattttg acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat
     4741 atcgcgacgt ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc
     4801 gtctcttctg accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat
     4861 cgcaataacc tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg
     4921 aagcgtaaca cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat
     4981 ctcggacttg gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc
     5041 aaagagaaaa cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag
     5101 atgaaagagt tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc
     5161 cgtttgccgc tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa
     5221 tactcagcac cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg
     5281 gttgacagtt gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac
     5341 tggtgtgtct cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca
     5401 acatggaaga gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc
     5461 aatgtcgata cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc
     5521 tttaacgtcg ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag
     5581 tcgtggggcc gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat
     5641 ccgctgccga agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc
     5701 cgtcctattc cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg
     5761 accaccacca cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg
     5821 ccggaacctg tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg
     5881 atcggcatgg tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag
     5941 gtcgcatagg gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa
     6001 aaaaacagcg cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat
     6061 cagaaagtaa ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt
     6121 tctgccagcg tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct
     6181 tccgccatca gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc
     6241 atcgtcgttt cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga
     6301 ccgacaaata ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt
     6361 tgattacctg tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc
     6421 tgcgcgtcaa aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca
     6481 tgttcacggc gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg
     6541 attgccgccg cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga
     6601 gcaatggctg cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc
     6661 gcctgcgggt caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca
     6721 ctaaaaccgc catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc
     6781 ccgcttttac acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga
     6841 ccatttttaa gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg
     6901 cctaaaaccc gtggataact aaaaaatggt tgatttccac cctaggcatg aatatcggta
     6961 ccgcaaatcc cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc
     7021 tcgcgttgtt tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac
     7081 atgattcggc tatccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg
     7141 gaacggaaaa tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat
     7201 ggactcacaa ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc
     7261 aggtcattga tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc
     7321 tggcggaaat gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg
     7381 aatgcggcgt cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg
     7441 atgacggttt cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg
     7501 cttttttcac gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc
     7561 aactggctcg ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg
     7621 gccgttataa tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc
     7681 aatcctacgc cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc
     7741 actttctcaa cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac
     7801 gtcatcggct actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca
     7861 gagattttca ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg
     7921 agatcatctc agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc
     7981 gcaatatcat tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg
     8041 accttgatgc cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc
     8101 gatccattaa tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat
     8161 tagcgggtgc acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc
     8221 acaatcagtc gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg
     8281 ccgttaatgt caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag
     8341 gagtccggcg tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct
     8401 tcgtcggcag atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa
     8461 cgcattcaaa ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac
     8521 cgcagttcgc tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc
     8581 gaaatcggcg ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc
     8641 ggcccactat tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg
     8701 tcactgttcc aggccatgtc tggcatggtg cacagcttta cgcagttcgt gttggtgcgt
     8761 atcggtatgg ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac
     8821 tggttcaaca tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt
     8881 ggcgttgctg taagcccacc gattctggcg gcaatgatgc tggtgatggg ctggcgcggg
     8941 atgtttatta ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat
     9001 cgcaaccgcg agcacgtaga actgactgcc gttgaacaag cttatctcaa tgcaggtagc
     9061 gtcaatgccc gccgagatcc gctcaatttt gccgaatggc gcagcctgtt ccgtaaccgc
     9121 acaatgtggg gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg
     9181 gcctggcttc ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg
     9241 atggcggcta tccctttcct gtttggggct gccgggatgc tggtcaacgg ttacgttact
     9301 gactggctgg tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattacc
     9361 gggatgttct gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgatg
     9421 gcggttctgc tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgctgg
     9481 ggcttgatcc acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac
     9541 tttgccagct tcatctgcgc ctctttcgcg ccgatcatta ctggttttat tgttgataca
     9601 acccactcat tccgtctggc actaatcatt tgcggttgcg tcaccgcagc gggagcactg
     9661 gcgtacatct tcctggttcg ccagccgatc aacgacccgc gtaaagatta aactccctat
     9721 gcccgataac attttgctta tcgggcattt ttatggcgat taaaacgttt cccagttctc
     9781 cccgctatct gccactgcca ttttacgcgg cgtagctggc gtcacggttt ttaccacagc
     9841 cgatgtttca cgctgctgtt gctgctgttg ctgaatccgg aacactgcca cagcttcggt
     9901 caggcgactg gcctgctctt ccagcgcggc ggcggcagcg gcagattctt ccaccagcgc
     9961 ggcgttctgt tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc
    10021 acggctctgc tcatcagaag cagaagctat ttcgcccata atgtccgtca cgcgggtcac
    10081 agcactgaca atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc
    10141 cactttcccc accgagtctt caatcaggct tttaatttca cgaaccgcct gggcgctacg
    10201 ctgggccaga ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt gctcacccgc
    10261 acgcgctgct tcaaccgccg cgttcaaagc cagaatattg gtctggaagg taatgccgtc
    10321 aattacgctg ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg
    10381 caccacgtta tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaatgccag
    10441 atggctggcc tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca gttgctccat
    10501 gctggctgcc gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcatt
    10561 attgccggta gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc
    10621 accgacggta cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat
    10681 ctcattagag ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat
    10741 gctgtcaatc aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc
    10801 gaagatgacc gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta
    10861 ggaggcattg ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc
    10921 cacatactgc ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt
    10981 gattttgcct gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta
    11041 attacgtttg atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc
    11101 gtaatccgcc cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc
    11161 agcaacggtt gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg
    11221 gttgagggtg ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tgggttgctg
    11281 ctggcgaatg gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaaaaa
    11341 cagaccgcct gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt
    11401 cacgatcttg atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg
    11461 gcctggaaag caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg
    11521 ctggttatca gatactaaaa agttattcat tatcgcccgg gatgtcttct tgtcgggaat
    11581 tgcccagagc aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa
    11641 actgagcttc aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg
    11701 cctgcccctc tttggtcagc gagatataga gcttccgctg atcattgatc ggctttaatc
    11761 gcaacactaa accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa
    11821 tgcaggcgcg atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca
    11881 cgatgcgcca ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca
    11941 tcgccgcttc tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc
    12001 tgaaaaattc aaaatcatta atatagaaac aattaatcat cacgttaccg ggactgagct
    12061 gcccccgcaa ttcttcattt tatcaaaatt gatgatattt tattaactta ctgatat
//
LOCUS       DAZOYF010000117        12114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZOYF010000117 DAZOYF010000000
VERSION     DAZOYF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.117"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..371
                     /locus_tag="ACHUOF_003003"
     CDS             <1..371
                     /locus_tag="ACHUOF_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612078.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="ACHUOF_003004"
     CDS             complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="ACHUOF_003004"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR0612079.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="ACHUOF_003005"
     CDS             complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="ACHUOF_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR0612080.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="ACHUOF_003006"
     CDS             complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="ACHUOF_003006"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR0612081.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="ACHUOF_003007"
     CDS             complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="ACHUOF_003007"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR0612082.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3449..5050)
                     /gene="tap"
                     /locus_tag="ACHUOF_003008"
     CDS             complement(3449..5050)
                     /gene="tap"
                     /locus_tag="ACHUOF_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR0612083.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5096..6757)
                     /gene="tar"
                     /locus_tag="ACHUOF_003009"
     CDS             complement(5096..6757)
                     /gene="tar"
                     /locus_tag="ACHUOF_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR0612084.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="ACHUOF_003010"
     CDS             complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="ACHUOF_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR0612085.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="ACHUOF_003011"
     CDS             complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="ACHUOF_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR0612086.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9395..10321)
                     /gene="motB"
                     /locus_tag="ACHUOF_003012"
     CDS             complement(9395..10321)
                     /gene="motB"
                     /locus_tag="ACHUOF_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR0612087.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10318..11205)
                     /gene="motA"
                     /locus_tag="ACHUOF_003013"
     CDS             complement(10318..11205)
                     /gene="motA"
                     /locus_tag="ACHUOF_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR0612088.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="ACHUOF_003014"
     CDS             complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="ACHUOF_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR0612089.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11913..>12114)
                     /locus_tag="ACHUOF_003015"
     CDS             complement(11913..>12114)
                     /locus_tag="ACHUOF_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR0612090.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2785 a         3388 c         2933 g         3008 t
ORIGIN      
        1 acactacgct aatgagttgg gatgccgtgc tctatgatct acgtagtgaa aaaattatca
       61 gtaaagataa ttacttgacc gacttaaacc gcagacatct tgaacttgtt cttcatacta
      121 accccaatcc aagcggttcg gtaacgaaga ttttacgcac tatttttact aagcaggtaa
      181 agagtttagg gccggaactc tgtcatttct tgcaccatga actgacgaaa aaacgttatg
      241 aagtatggca tcactacgat gtactgcact accagtcatc gtcattttct cgttcacaat
      301 ttaatgcatt gataaattta ctggcgcaac aatcacgctt cgctgatcta aaattatcaa
      361 actacaaata accatcctcc acgtactccc tgaacgatac ccgccaccct gctatcggct
      421 tggcatcatc gcgcaggccg aagttcaggc aatacaaatc aaaatccaag actatccaac
      481 aaatcgtcca cctgatcctg actggctacc acgccggctt tgctggtatc gacctgaggt
      541 ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc
      601 aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaata
      661 acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta
      721 gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca
      781 cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct
      841 tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc
      901 cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg
      961 atggcttccg ccgcttcggc aatggcctga accagcccca gttcccgcaa actgtcgcgc
     1021 agcatatgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca
     1081 ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt
     1141 gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc
     1201 cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg
     1261 caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatatt
     1321 gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc
     1381 gtcgacacca tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg
     1441 cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc
     1501 catttcacac tcctgattta aatacgtatc gcctgtccgg cactaattgt tgccaacatt
     1561 tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg
     1621 cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc
     1681 tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag gatcacccca
     1741 accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga
     1801 tgacggttaa ccgccgggcc gtcgtgaatt ttgatttgat aatttgcgcc actacgcgcc
     1861 agttccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaagac acgttctccg
     1921 tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg
     1981 gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg ggctggaaag cggcaacggt
     2041 tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt
     2101 tcagaactca acaacggccc tgccttcagc gttgtcggtg ctgacaatgg cttatgtgct
     2161 gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct atacgccagc
     2221 attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctattgcccc cagctccagc
     2281 gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag aaaccatcac aacgggcatt
     2341 ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca
     2401 tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcaac cagcggatca
     2461 ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg
     2521 cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac
     2581 ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcgaggtgg ctaaagtttt
     2641 cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa
     2701 tctcctgctg ggtagtttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa
     2761 acggccccgg cacggtgtac tgtttcgcca gtaaattcag cggggcaaaa tcaacatagt
     2821 tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat
     2881 accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgatag ataccgcttc
     2941 tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggca
     3001 cggtgcccaa tgtgtcagcc agagtcatcg caatgctgta cggctcttcg ccggtcgaag
     3061 ccgccgcgct ccatacgcga tcctcgccag aacggcgacg tgcgtgatcc gcgagcagag
     3121 ggaaatgatg agcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg
     3181 cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg
     3241 tcagtcccag agaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt
     3301 cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt
     3361 cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag
     3421 atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg
     3481 ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa
     3541 gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct accagcgagg
     3601 agttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc
     3661 gccgttgttc ttccgacgcc gaagcaattt ctcccataat gtcgttcacg cgggtcaccg
     3721 aactgacaat atcggtcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga
     3781 cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc
     3841 tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc
     3901 gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga
     3961 taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag
     4021 tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt
     4081 ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac
     4141 tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag agatcgttat
     4201 tgcctgcgac aatctccgcg atcccaatgt gcatctcatg gcttccctta cgcacatcac
     4261 tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct
     4321 cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat
     4381 ggctaccgat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc
     4441 tgatgtagat cgccgcaaca ataatcatgc tgataaacac cagcgccgaa atctgatagt
     4501 tacgctggct ttgcgcactg gcggcttcca gcacatggtt gatctccagc tgccaggcct
     4561 caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg cgcagtgagg aaatccgaaa
     4621 gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac
     4681 ttttctccgt ctctttttgc aatgccctga cgtgctcgtt gcccgcagtc atcgccataa
     4741 aacttttaaa cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt
     4801 taatgtcatc cgccggatag ctaagcgctg tcagagtgcc tgctttgttc agcgcggtac
     4861 tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct
     4921 cgccgtgatt cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc
     4981 caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac
     5041 gattaaacat caacgctccc catcaggcgg caatgaccgc gttagtaaaa actcgtcaaa
     5101 atgtttccca gtttggatct tgttcagtaa ttcgcagtcg tggctgtgcc ggtggttgct
     5161 cactggcagg acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga
     5221 acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc cagcgcagct gcggcggcag
     5281 ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca
     5341 atgcgacttg atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa
     5401 tgtcagtcac gcgagtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga
     5461 ccagcaccga accggtatca atgcgcgaga cggagtcttc aatgagggct ttgatctctt
     5521 ttgccgccag ggcgctgcga ctggcaagat tacgcacttc acccgccacc acggcaaaac
     5581 cacggccctg ttcacccgca cgcgcggctt caaccgcggc attcagcgcg aggatattag
     5641 tctggaaggc aataccgtcg ataacgctga taatgtcggc aattttcttc gaactatcgg
     5701 cgatctcatg catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt
     5761 cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg
     5821 tcgcggtgag ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag
     5881 tacgggagga aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat
     5941 ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc
     6001 tctgcgccag gtcacccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac
     6061 caccggcgat ttcgcaaatg tgagcaataa tttttgccag cggagtaagc aacatacggc
     6121 gaatgccgta ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt
     6181 gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt
     6241 cactgctgag ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg
     6301 gctgagcgaa ataagctcca gtattgccat aatcaagata atcaatcagt tcagttaacg
     6361 ctgtgtaata gtttttatat ttttcatcaa aattacggct ggtagcgacc atttcaggta
     6421 acggtgccat gcttttgaat tttttataat gcgtcgcggc ctgggccaat gttttcctgg
     6481 cgctatcgag caattcaact ttggcgttac tttgttgatt ggaggaatcc atcatcatcc
     6541 gcaccgctga acgactcagg ttaatgcgcg tttgcagcat taaatcccag gttgacgtca
     6601 gctcgccctg ctgttcccgt aattgattgg aaaccacaaa gctcttctgg ctatggtgaa
     6661 gggaagaaaa aaacaaactg ccggaaataa gctgtaacag tgcgaatacc cccagcacca
     6721 ttaccaacag cgtgactacg cggatacggt taatcataag gcaccttcct gaaaacaagt
     6781 tgatctcgtt atcggcaaga aggggggaaa ctttattgct gatgccaccc gccgcgaaat
     6841 tgaaataaaa aacccgatgc gcagatcatc gggttcattt caattgagga aatcgggaga
     6901 attacgccac ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt
     6961 tttcgatgtt caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt
     7021 ctgttgaaag cgtcacggca aactctggtg ccggacgaat ttgctccgcc gtcaatgaaa
     7081 gcacgtctga aacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga
     7141 taactaccgt gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa
     7201 caatcggcac aataacgccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg
     7261 caatccgtgt tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt
     7321 actcttcatc accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct
     7381 ttgttacatt cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt
     7441 cgccatacgt tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac
     7501 gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc
     7561 aaggttttta accacaacct ggtgttgacc aattaattga tccaccagca aggcgtagcg
     7621 gcgaccgcca ctttgtaaga tcaccacaat tccctgggtg gcttcggttt tcgcgcccgc
     7681 gacgttgaac actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac
     7741 ccgctcgccg ccggccagtg gatggagatc ggcttcacgg ggttgcagtg attccataac
     7801 agcattcagc ggcagaatga aaacttcatc cgcaacgcgt acggacatgc cgtcgaggat
     7861 ggccagcgtc agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat
     7921 ttcgacatga ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg
     7981 cccggagacg tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcattgc
     8041 aacttcgtcg tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag
     8101 aattcgctca cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc
     8161 ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt
     8221 ttctggcagt tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat
     8281 aatgcgttct atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac
     8341 ctgcttgccg agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata
     8401 ttccatcggc atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg
     8461 taactgcccc atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg
     8521 ctgggcaagc atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac
     8581 cttttctacc gctacacgga tgctggtgga ttcactgctg cgcgtcgttt tttcccgctc
     8641 cacgcggccg gttggcgctt gttcggctgc cagtttaagc acaggtgggg tagctatttt
     8701 tggcgaaact tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac
     8761 cgctgtgatg tcatcttcgg cgatatcgcc cggtaatatt gccgagagag aatccgcccc
     8821 tttcaccacg tcatttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc
     8881 gccggccttc aggcgcgaaa ggataattcg tcgcggcaac tgactgcgac tttgctcatc
     8941 ttgcggttca cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt
     9001 cgcttctaat gccagttgac gcaaggcctg gcagatataa tcgtagctgg cggcatccgg
     9061 ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg atatccttcg tttccaaaaa
     9121 cagattgata atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt
     9181 ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt
     9241 gatcgagtgg gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg
     9301 caaaaccagc agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt
     9361 ctgataaaaa tcgcttatat cgatgctcac gctgtcacct cggttcggct gatggcattg
     9421 tgggaacact gacctgtggt gcaacctcag gtttttctag ggcgcttact ggctcattct
     9481 ggctttcggc gttttcatgc aaaatggcct gttcggcttg tttgttcagt accaacaggc
     9541 tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca
     9601 tgccgacgac acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg
     9661 atgcattggc ccgatcggca gaaagctccc agttgctgta acctttctca ccgctggcgt
     9721 agggaaaatc atcggtatga cctgaaaggc taatacggtt gggaataccg ttcagcacag
     9781 gcgcgatggc gcgcagaatg tcgcgcatat agggttcgac atcggcactg ccggttctaa
     9841 acatcgggcg attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga
     9901 ttttgagatg gagacgtaac gcccgcagtt tcggatcgga ctctatcaac tggtcgagat
     9961 caccccgcaa tttccgcaat cgactttgct ccatgcgttt tttcagctct tcgatgttcg
    10021 gctgcttatt cacttccccc tggctttggg tgtaatcatc accaccgccg ggaattgggc
    10081 tttcactatt ggaaatgcga tcgccgcccg taaccgctgt cgccagtgga gtccggaagt
    10141 actccgcaat ctgaatcaac tcttttgggc tggagatgga gatcagccac atcaccagaa
    10201 aaaaggccat catcgcagtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg
    10261 ccccgtggct tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca
    10321 tgcttcctcg gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag
    10381 ttcaataaac gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat
    10441 aggcggtgcg taaccgttca gattagaaag cagagtgact ttgacgcact gcatcatttt
    10501 gctggtttcg gcgcttttct gacgtaaaac actcgctagt ggggaaataa atccgtaagc
    10561 caataaaata ccgaggaaag tccccaccat cgcatgtgcg ataagcgccc ccagctcggc
    10621 ggcaggacga tcggctgaac ctaacgcgtg aacgcccccc attacagccg cgacaatacc
    10681 aaacgccgga agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct
    10741 ttcgtgcgtc tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc
    10801 gctgataatc aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgaggat
    10861 gcgtgggtag ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga
    10921 aaacatcccc atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat
    10981 atacattgct ttggtgtatt tggagcgacg aaacagcaac ggcagcgctt tcagcgtgcc
    11041 tttaatcgct ttgccattat tgccgacgat aaacgaccca atccctgcac cggcaataat
    11101 caccagttca gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc
    11161 gaaaactgta ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca
    11221 ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggaaaaaaaa
    11281 agcagcggta cgtcgttacc gctgctggaa tgttgcgcca caccgtatca gttaaacagc
    11341 ctgtactctc tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt
    11401 acgtcttttt actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg
    11461 ctggtgagcg tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa
    11521 tccactttca acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gtggtccttc
    11581 ttctgcttgt gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc
    11641 attacacaaa ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac
    11701 gttttgttcc caggtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg
    11761 gcttccgcgc agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct
    11821 ttccagcatc tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg
    11881 cgcttcctga acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct
    11941 tcaggctgat taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc
    12001 tggagatcgt caacgcggga atcttgcgtc aactgagtaa tcgtctggtg gctgtcaaaa
    12061 cggaagtgac aaaccagctg attggtttct gccagcttaa ccatttgcgg aaga
//
LOCUS       DAZOYF010000118        12072 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZOYF010000118 DAZOYF010000000
VERSION     DAZOYF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.118"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="ACHUOF_003016"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="ACHUOF_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR0612091.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="ACHUOF_003017"
     CDS             complement(2005..2928)
                     /locus_tag="ACHUOF_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0612092.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="ACHUOF_003018"
     CDS             3145..4488
                     /locus_tag="ACHUOF_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0612093.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="ACHUOF_003019"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="ACHUOF_003019"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR0612094.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="ACHUOF_003020"
     CDS             6508..6732
                     /locus_tag="ACHUOF_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR0612095.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="ACHUOF_003021"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="ACHUOF_003021"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR0612096.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /gene="ydcK"
                     /locus_tag="ACHUOF_003022"
     CDS             complement(7326..8306)
                     /gene="ydcK"
                     /locus_tag="ACHUOF_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR0612097.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="ACHUOF_003023"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="ACHUOF_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR0612098.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="ACHUOF_003024"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="ACHUOF_003024"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR0612099.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLTRKK"
     gene            10315..10971
                     /locus_tag="ACHUOF_003025"
     CDS             10315..10971
                     /locus_tag="ACHUOF_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFR0612100.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="ACHUOF_003026"
                     /pseudo
     CDS             complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="ACHUOF_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11503..12072
                     /locus_tag="ACHUOF_003027"
                     /pseudo
     CDS             11503..12072
                     /locus_tag="ACHUOF_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
BASE COUNT         3126 a         2825 c         3031 g         3090 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctgaca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatataac aattactctg atttaaaaga aacaacctca
    10441 gcaacaggta aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac
    10501 agcatcgtct ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg
    10561 cagaaagttc tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg
    10621 cagcgtaaac cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt
    10681 accggtgtag ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta
    10741 gtggttgcgg ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt
    10801 gaaggtgagc tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc
    10861 gaaggtaaag acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt
    10921 attgatgaca tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg
    10981 cgccatcgtt agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg
    11041 ggatataagg cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg
    11101 atgacggata ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg
    11161 cctttgtagt aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat
    11221 acgcctttga ggctgtttac cagactggat atcgccagtt tgggaggtta gttaattaac
    11281 aaatggacgt gatctggttc gccatccatt tcaaccagtt cagcttcaaa ataagcacat
    11341 acatttgaaa agtaagtgcg tagtttttct gtcgcgtcat ggtcaaaaat ctggcgtcgg
    11401 tatctggtga caaagactag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg
    11461 atatcggttt cttttttcac agaccaaagt atcatttgac ctgtgaaacg attacaggca
    11521 tttaaattcc agttaagacc aggtggtcaa caggagtgtg aaatgaggcg cttcgcaggc
    11581 gcatgtcgtt tcgttttcaa tcgtgctctg gcacgtcaga atgagaatca taaggccggt
    11641 aataaataca tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaatgccact
    11701 gaaacgcaat ggcttaaaga ttctccctca cagccattgc aacagtcact gaaagacctt
    11761 gagcgggctt acaaaaactt cttccggaag cgggctgctt ttccccgatt caaaaagcgg
    11821 tgacagaatg atgcattccg ctacccgcag ggcgttaagc tcgatcagga aaacagccgt
    11881 atttttctgc cgaaactggg ctggatgcgc taccggaata gccgtcaggt cacgggtgtt
    11941 gtgaaaaatg tcactgtcag ccagtcctgc ggtacgtggt acatcagtat tcagacagaa
    12001 agtgaagtat ccactccggc tcacccttca gcatcaatgg tcgggctgga tgctggcgtg
    12061 gctaaactcg cc
//
LOCUS       DAZOYF010000119        12054 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZOYF010000119 DAZOYF010000000
VERSION     DAZOYF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12054
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.119"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(5..508)
                     /locus_tag="ACHUOF_003028"
     CDS             complement(5..508)
                     /locus_tag="ACHUOF_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR0612101.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(553..1516)
                     /locus_tag="ACHUOF_003029"
                     /pseudo
     CDS             complement(553..1516)
                     /locus_tag="ACHUOF_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(1830..2966)
                     /gene="hemW"
                     /locus_tag="ACHUOF_003030"
     CDS             complement(1830..2966)
                     /gene="hemW"
                     /locus_tag="ACHUOF_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR0612102.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(2959..3552)
                     /locus_tag="ACHUOF_003031"
     CDS             complement(2959..3552)
                     /locus_tag="ACHUOF_003031"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR0612103.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(3560..3850)
                     /gene="yggU"
                     /locus_tag="ACHUOF_003032"
     CDS             complement(3560..3850)
                     /gene="yggU"
                     /locus_tag="ACHUOF_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR0612104.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(3847..4413)
                     /gene="yggT"
                     /locus_tag="ACHUOF_003033"
     CDS             complement(3847..4413)
                     /gene="yggT"
                     /locus_tag="ACHUOF_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR0612105.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(4431..5135)
                     /gene="yggS"
                     /locus_tag="ACHUOF_003034"
     CDS             complement(4431..5135)
                     /gene="yggS"
                     /locus_tag="ACHUOF_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR0612106.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            5153..6133
                     /locus_tag="ACHUOF_003035"
     CDS             5153..6133
                     /locus_tag="ACHUOF_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR0612107.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            complement(6309..6725)
                     /gene="ruvX"
                     /locus_tag="ACHUOF_003036"
     CDS             complement(6309..6725)
                     /gene="ruvX"
                     /locus_tag="ACHUOF_003036"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR0612108.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(6725..7288)
                     /locus_tag="ACHUOF_003037"
     CDS             complement(6725..7288)
                     /locus_tag="ACHUOF_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR0612109.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(7397..8347)
                     /gene="gshB"
                     /locus_tag="ACHUOF_003038"
     CDS             complement(7397..8347)
                     /gene="gshB"
                     /locus_tag="ACHUOF_003038"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR0612110.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(8360..9091)
                     /gene="rsmE"
                     /locus_tag="ACHUOF_003039"
     CDS             complement(8360..9091)
                     /gene="rsmE"
                     /locus_tag="ACHUOF_003039"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR0612111.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(9171..9878)
                     /gene="endA"
                     /locus_tag="ACHUOF_003040"
     CDS             complement(9171..9878)
                     /gene="endA"
                     /locus_tag="ACHUOF_003040"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR0612112.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(9973..10470)
                     /locus_tag="ACHUOF_003041"
     CDS             complement(9973..10470)
                     /locus_tag="ACHUOF_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR0612113.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            complement(10547..11941)
                     /gene="galP"
                     /locus_tag="ACHUOF_003042"
     CDS             complement(10547..11941)
                     /gene="galP"
                     /locus_tag="ACHUOF_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR0612114.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         2955 a         3090 c         3063 g         2946 t
ORIGIN      
        1 tccattaact ggcctcctgc gcaggttgtt tcagttgttg aatgctttga ttaataaaac
       61 acaaggcata aaacatcatt accactccac ttaatggcag aatgatatat acgtatccca
      121 tcggtatttg catagcggca gatacctgtg atgttgccag tgttttatca attaatttca
      181 ggccaccagt cacaatgacc gaaccggcaa aacctaaaat gagaacgtta atgactatac
      241 taagaagtaa ttgtttgcgc ttattcaggg cgagagcaaa taaatcaata gagagatgtc
      301 gctgcgcgcc gacggtatat gcagcaccta ataatcctac ccagatcatc aaaaagcgtg
      361 ccagctcatc ggttaatgta cttggctggt taagtacgta gcggctaaat acttgccaga
      421 ctacgcatac aaccagagca agcattacgg cgacagaaaa cgtggcaatg acgcggtcta
      481 ccgcaagttt caggcggtta aatatcattg taagcatcct ggaaaatcag ggccagaggc
      541 tggcccggaa tattattgag cagcgttgtc gaatttctct aacaaagcag cttgttttgg
      601 atcttttttg aagtcgtcaa acagaggttg cacggcttta cggaatggcg ctttatccac
      661 ttttacaatt tcacccccca tagctttcgc ctgagcacgt gtgtcagcgt caattttttc
      721 ccacaatttt tgttgataag cttcggattt cttcgccgct gtttcgagga tttgttgctg
      781 ttctggggtt aatttattcc atgttttggt ggagataacc aaaaagtcag gaattgaggc
      841 gtgttcatct tcagaaaaaa ctttggcaat ttcaatatgt cgagtctgca tccaggaagg
      901 cacgttattt tctgcgccat ctactacacc ttgctgcatc gcagtataaa cttcgccaaa
      961 tgaaattgga gtgggtgaac cacccatcag ttcaatcatt ttgatggtgg tcggacttgg
     1021 ctgaacacga attttcagtc ctttaagatc ttctggcttt gtaatcggtt ttttcgcata
     1081 gaaactgcga gtacccgcta cataagctga taaagcaaaa aagcctttgt cttttgttga
     1141 gtccataatt tctttaccaa cttcaccaaa aacaacttaa taagaacggc aaattataga
     1201 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
     1261 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1321 tacggatcac catattacct tttgacagct gttttacctc tttcgccagc tcttcaaaac
     1381 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgcc actttttctg
     1441 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1501 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt gatatctaat
     1561 tgtgcttatc agtgatttgt gcgacaaatt ggttaacctg tctgacaact tgtctatgtt
     1621 tatcacaact tgaaataaag tcagaaatta aatattaaat ttgtgcgcca ggttgtacat
     1681 atttataatc aatgaatgat tagtaaatat tcactaaata tatataataa atgtcgtcga
     1741 tatttattca gtaagaaata tatttctgcc gaggttaatt aacgggggga taaaagaagt
     1801 gattaatgcg cccgcaggcg cattaatgat tactcagcga ggaataattc aagaagtgag
     1861 tttaagaata atttaccatg ttccgttatc tgccagtaat ccgcacattc ggtgagataa
     1921 ccctgggcaa tcgcctcgtc taactgtggg cgaatcacat cttcgcaaag cccggtatac
     1981 gcaataaact ccacgcgcgg cgcggcctcc agcagacgga agcgattcat aaagaactca
     2041 aacggcttat ctgcggcttc gacatcacgc tggctttcca gataccttcc ttgcataaaa
     2101 ccacgaggat gacgcgtttt agtggtacgc agaatgcgcc catccgggaa ggtcactttg
     2161 ccgtgcgcgc cgcagccaat accgatgtag tcaccaaagc gccagtagtt gagattgtgc
     2221 tggcactgat aaccgggttt ggcgtaagcg gaagtttcat attgctgata acccgctgcg
     2281 gttaataact gatgcccctg ttcgaaaata tcccacagcg cgtcatcgtc cggcagcacc
     2341 ggcggtcgcg agccaaacag cgtattaggt tcgatagtca gttgatacca ggaaagatgc
     2401 ggcggattca gttcaatggc ctggcgtaga tcgccaagcg cctcttccag tgattgatcc
     2461 ggtagtccat gcatcaaatc aaggttaaag ctacgtaacc ctaaaccgct cgccagcttt
     2521 gccgctcgtt tcgcttcttg cgggccatga atacgcccaa gtcgtttcag cttttcttcg
     2581 ctaaaactct gcacaccaat agagatgcgg tttacaccag cacgctgata atcgacaaag
     2641 cgatccgctt ctaccgtgcc tgggttcgct tccatagtaa tttctgcatc cgctgccagc
     2701 ggcaaacgcg cacgcacgcc gtctagcagc gtttgcatcg ccgggccgga aagcaggctc
     2761 ggcgtaccac cgccaataaa gattgtcttt acttcacggc cctgagcgta agccacatca
     2821 ttgtccagat cgcaaagcag atgctgaaca taatcgtcgt gcggcacttc tcctttcaac
     2881 gcgtgagagt tgaaatcgca gtacgggcat ttctgcacgc accacgggat gtgaatgtag
     2941 agactcagcg gcggtaattt aaccattacg taaagcgtcc agcagcagtt tcaacgcctg
     3001 accacggtgg gaaatggcgc tcttttcttc gcgggtcagt tcggcggcgg ttttcccttc
     3061 ggaaggtacg aagaagattg gatcataacc aaagccacca gtgcccgctg gttcgcgagt
     3121 aatcacgccc ggccagctgc cgtggcatac cagcggagtc ggatcttccg cgtgacgcag
     3181 atataccagc acgcagtgga aacgcgcctg acgttggtcg tccggtacgt ctttcagtgt
     3241 ttccaacagt ttttgcagat tcttttgatc ggtcgcgtct tcaccggaat aacgcgcgga
     3301 gtaaataccc ggcgcgccac ccagcgcatc taccgccaga ccagagtcgt cggcaattgc
     3361 cggtaaaccg gtcacttttg ccgcatggcg cgctttcaga atcgcgtttt cgataaaggt
     3421 taggccggtt tcttcagcgg aatcaacgcc gaggtctgtt tgggccacga tatcaagacc
     3481 aaagtcgcta agcagcgacg ccagctcacg cactttaccg gcattgccgg ttgcgaggac
     3541 aactttttgc ataggatacc taattaatta acgccgcgat ttctggcggg atttgttgcg
     3601 gattaatgat tttaatttgt ttgtggcggc caagttcgcc tttttcaatc accacctggc
     3661 ttttggcaac ccggaattgc ttaccgagaa acttcaccag atgactgttg gcctggccgt
     3721 caaccggcgg cgcggtaatg gcgactttaa cttcgtcgcc atgtaaaccg acaatagaat
     3781 cacggctggc tttcggctga atatagagcc gtaaaaccag accgtcatca ttaactgtta
     3841 cggcactcat aacgccatcc acagccccgg cagcagcatg tttccggttg cctgtaatac
     3901 ttctgcgaca cccatattga tggcatacag cagcagaacg aggatcatcg gcgagaaatc
     3961 aatcccaccc attgccggta gcaagcggcg aatcgggcgc agcagcggat cggccagctg
     4021 aatcagcacg tattcaatcg ggctacgacc ctggcttacc cagctcataa tcgccatcac
     4081 cagcaggacc cagaaaatca gcaagccgat ggttttcagc agaatcagta aaccggcaat
     4141 ccagatgatt ggcaggaagg tcaccacttc aaacagcaca atggctttga taaaactgag
     4201 aatataggca accagcagcg aggcgctgtc aatgggaccc attgccggaa taacgcggcg
     4261 cagtggcccg ataattggct gcgctacctt cactacaaac tgtgagaagg gggtgtaaaa
     4321 atcacaatga gcacactgca tccagatgcg taataacagc accattgtat acagctcaat
     4381 gaccgttgaa agcaggaaag tcaacgtatt catggcgttc ctttaattcc ttatttttta
     4441 gagtaatcac gcgcaccaaa aattgcagtg ccgatacgaa ccatcgtgct acctgccgca
     4501 atggcggctt ccatatcgtc cgacattccc agagagagcg tgtcgatatg cgggtagcgc
     4561 gttttcagtc cggcaaatgc tacagccatt tggcgtgcaa cttcaaactg ccttacatat
     4621 tctgactcaa gcgcagggat tgccatcagc ccgcgcagac gtaaacgcgg tagttcagcg
     4681 accgccgctg ccagctcgtc cagttctgcc agttgaatcc cggacttact gttttcatca
     4741 ctaatattaa tttgaatcag tacgttaaga gggggaagtt ctgccgggcg ctgctcgttg
     4801 aggcgggtag caatgcgcaa acggtcgatg gtatgacacc agtcgaaatg ctctgccact
     4861 aggcggcttt tattagactg caacgggcca ataaaatgcc attctaatcc tgttacgccc
     4921 agttcctgaa agtggcgaat tttatctacc ccttcctgaa cgtagttttc accaaattga
     4981 cgctgcccgg catcaatggc ttctgcgatg gcgctcgcag gttttgtttt actgactgca
     5041 agcagcgtaa tttcttctgg agaacggccg caacgcgttg cagccgctga gattttgtcc
     5101 cggacctgtg ccaggttatg cgcaatatcg ttcattttcc gaggatctta gtatgaatat
     5161 ggaagaaatt gtggccctta gtgtaaagca taacgtctcg gatctacacc tgtgcagcgc
     5221 ctggcccgca cgatggcgca ttcgtggcag aatggaagct gcgccgtttg atgcgccgga
     5281 cgtcgaagag ctactgcggg agtggctgga tgacgatcag cgggcaatat tgctggaaaa
     5341 tggccagctg gattttgccg tgtcgctggc ggaaaaccag cggttgcgtg gcagtgcgtt
     5401 cgcgcaacgg caaggtattt ctctggcatt acggttgtta ccttcgcact gtccacagct
     5461 cgaacagctt ggtgcaccac cggtattgcc ggaattactc aagagcgaga atggcctgat
     5521 tctggtgacg ggggctacgg ggagtggcaa atctaccacg ctggcggcga tggttggcta
     5581 tcttaatcaa catgccgatg cgcatattct gacgctggaa gatcctgtgg aatatctcta
     5641 tgccagccag cgatgtttga tccagcagcg ggaaattggt ttgcactgta tgacgttcgc
     5701 atcggggttg cgggctgcat tgcgggaaga tcctgatgtg attttgctcg gagagctacg
     5761 tgacagtgag acaatccgtc tggcgctgac ggcggcagaa accgggcatc tggtgctggc
     5821 aaccttacat acacgtggtg ccgcgcaggc agttgagcga ctggtggatt catttccggc
     5881 gcaggaaaaa gatcccgtac gtaatcaact tgcgggtagt ttacgggcag tgctgtcaca
     5941 aaagctggaa gtggataaac aggaaggacg cgtggcgctg tttgaattac tgattaacac
     6001 tcccgcggtg gggaatttga ttcgcgaagg gaaaacccac cagttaccgc atgttattca
     6061 aaccgggcag aaggtgggga tgttaacgtt tcagcagagt tatcagcagc gggtggggga
     6121 agggcgtttg tgacgttgcc atgcccgatg cgcaagttta tagggcctat aaaggcaata
     6181 aatgtctgat gcgacgctaa cgcgtcttat cagacctaca gccgctgcca gatgtgtgct
     6241 ggataaggcg ttcacgccgc atccggcaag atggcggtaa cgactatccg ccaggctttt
     6301 agatcgcctt aatatccctg ctcgaaatag ctttcgagaa taataaccgc agaggcagag
     6361 tcaactttgc ctttgttgag cgcccgatag ccgccctgtt caaaaagacc ggaacgggct
     6421 tccacagtgc taagacgctc gtcatggagc tttacttcaa caccgaaacg accatgaata
     6481 cggttggcaa atttgcgcgc tctggcggtt aacggctgtt cggtgccgtc catattcagc
     6541 ggcaaaccga cgatgatttc gtcaggctgc cactctttca gtaaacgttc gatgaggttc
     6601 cagtccggcg taccgtcctg cgctttaatt gcaggcaaag ggcgagcggt gccggtaatg
     6661 cgttggccga ccgctacgcc aatgcttttg gtgccgaagt cgaaggcgag taaggttcca
     6721 ctcatcaggc gtgtcctgcc acaccaggca tggtgagaat atccacacca atcagttttg
     6781 ccgcctcgcg ccagcggtcg gcaatcggcg ttttgaacag aatattcaga tccgccgggg
     6841 ccgttagcca cgcgttatcg agaatttctt gttccagttg acctttttcc caggaggcat
     6901 agcccagagc caccaatacg tcagacggtt gtttatcggt gccgagcgtt tccagcacat
     6961 cgcgggaagt ggtcatcacc gtgttgtctg aaatgcgaat gctggaagca aaattggagg
     7021 gcggagtatg caaaataaac ccgcgatctt cagccagtgg gccgccgagc ataaccggtt
     7081 tatccagacg gattgattca tcacgcggct ccggcgtgat cttcagcttt tccagaatcc
     7141 cttcaatttt gagattttcc agcggcttgt tgacgatgat ccccattgca ccattggtat
     7201 tatgttcgca aatgtagacc acggaacgac ggaaaatcgg atcctggaga gcaggcatgg
     7261 caataagaaa gtgatgctgt aaattcattg tcagaggttc tgtttcctgg ttcaaaaaag
     7321 caacagcgcc cagtatgcgt ggaaagaagg gcgctgtcac tcagagtctc aacgagatcc
     7381 ttctcgctaa ggtgggttac tgctgctgta aacgtgcttc gatggcatcc attaacattc
     7441 cggtgatcga caccggaaac tctgcttcaa tctcacgaat acaggttggg ctggtgacgt
     7501 taatttcagt cagacggtcg ccgatgatat ccagaccaac aaaaatcagc cctttttctt
     7561 tcagcgtcgg cccgatctga cgggcgattt tccagtcact ttccgtcagc ggacgaggtt
     7621 caccgcgacc accggcagcc agattgccac gggtttcgcc cccctgcgga atacgcgcca
     7681 ggcagtacgg taccggctcg ccatccacca ccagcacgcg tttgtcgcca tctttaatgg
     7741 ctggcaggta attttgcgcc atgcagtagc aagtgccatg ctcagtcagg gtttcggcaa
     7801 tcacgccgag gtttggatcg ccttctttca cgcggaaaat cgacgcgccg cccataccgt
     7861 ccagcggctt aagaatgatg tcgctgtgtt tctcccagaa cgcttttagc tgcgctttat
     7921 tgcgcgtaac cagcgtttct ggcgttaagt cagagaacca ggcggtaaac agtttctcgt
     7981 tacagtcgcg caggctctgc ggcttgttaa cgatcagcgt ccctttctct tcggcacgtt
     8041 ccagaatata ggtcgcgtag ataaactcgg tatcaaacgg cgggtcttta cgcatcagga
     8101 tcacatcgag atcggccagc ggcagatcct gttcaccgac gaacgaaaac cactcttcgt
     8161 agttctgctt cacgttcagc gtgcgggtat gggcgcgggc ttcgccattg atcagataca
     8221 gatcgcccat ctccatatag tgaagttcgt aaccacgacg ctgtgcttcc agcaacatag
     8281 cgaaactgga atctttcttg atgttgatgt ttgcgatggg gtccatcacg atgccgagct
     8341 tgatcattat tcttctccgt tagcccaaat cgccaaatcg tacttgtagc gcggtaatgg
     8401 cggtgagcgc agttgtctct gtacgcaaaa cgcgaggtcc caacaggata tcagtaaatt
     8461 gatagcgggc agtcatggca atttcatctg ccgataaacc gccttccggg ccaatcagca
     8521 ggcggacgcg ttcaaccggt aaaggcaacg tattgatgct gttactggcg cgcgggtgaa
     8581 gattcagttt cagtccttca tcctgctctg cacaccaggc ttccagatcc atcgctggac
     8641 ggatttccgg cacccggtta cgaccacact gctcacaggc agcaattgca atcttctgcc
     8701 actgctgaag cttcttgttc agacgttcac tatccagttt aacgccgcag cgctcagaaa
     8761 aaagtggcgt aatgaggctt acaccgagtt cgatcgattt ctggatagta aattccattt
     8821 tttcaccacg cgacatcacc tgaccgaggt gaatatgcag cggagattcg cgatcgtcga
     8881 tctggccttc cagcaccttc acttccacgc tttttttgct ggcgctggta atttcggcgt
     8941 caaagacctg gttgctaccg tcaaacaatt gcaacgcctg ccccggcccc atgcgcagta
     9001 cgcgcccgat atggttggcg gcatcttcgc aaagcgcgat gtgagaatgg ctggtcagtg
     9061 gttcaggatg ataaatgcgg gggatacgca tagttaaaaa tccgcgtcgt ctccccacgc
     9121 ggttgtacgc gtggggtagg ggttaacaaa aagaatcccg ctagtgtagg ttagctcttt
     9181 cgcgcctggc aagcgcgttg cacatacggg ttatgattgc cctgcacctt cgcgatgcgt
     9241 tcatcgcgct cgcactccca gtcggtaacc ggatacatct tgttccatgc gttgaacagc
     9301 tgcgtttgct ggcgagagag tgtcaggttg tattggtcgc gcatatagaa gtaggtgcgc
     9361 gcaatggcac cgcgtgcacg cgctggtggt tcggcagctt tttctttgaa atcgaccttc
     9421 atggcgcatt gaccgtactg gccttcgccg ccattccact ggctgtacat aaaattgccg
     9481 cgatcgccat tcacctcgcc gactgacggc tgcaggttat gcatatcgct ttccatcttg
     9541 cgatagaccg gatctttagc gcagttttta cgtccaccgt cctgccagca ctggcgctgg
     9601 tgaccgaatt gccaggcggg aacgacgtgt tcccactcta cgcggctggc gcggttttca
     9661 tttttgcgca cctgatagcc gcacgattgc agatcaacaa cgcctttttt gccctgccag
     9721 ttaattttac atccgcaata aaacgtaccg ggcgcatcag cgtggacttt taccgccgcg
     9781 gctttcgcct gagaaaaact attgatacct tcggccaacg tcgggccgga aaatgctgcg
     9841 ctcagtacca ccgcagcaat agacaaataa cggtacatca cttactccgt gttaaaacga
     9901 gccagcaacg tagcgaaagt tgttttggta tgcaatcagc cagatcagga aagttcctga
     9961 tagttcaggt ggttatttcg caaccagctg ttcaccgcag tgaacacagc gatagatggc
    10021 ctcgccacga actacgcgat tatggcggcg tacggtaagc tgatgttcct ggcacttaca
    10081 gcggtagggg aaggtgttgc gacgcacgga ttgcagttcg aactgatgcg tacgacgggc
    10141 gggaacgccc agcacgcttt ccatcatcca cttccactct ttgccatgtg gcgctacgcg
    10201 gccgaaatgt ttccatacca gcaaatgtgc cagttcgtgc ggtaccactt cttcaataaa
    10261 agcttcactg ttttccaaca gcaaaacggg attgaggcgg atttcatagc tttccagcca
    10321 ggctgttccg gcggaggttc cgcgctgggt gtaagagagt tttggctccg ggtagttacg
    10381 ccctagcttc aggttggcct gggcgagttt ttcccgcagg cggcgcataa cggcctgttg
    10441 gatggcgata gggagacggg atgttttcat agcggcagag gatagagcga agaaaagatg
    10501 actgcaagag gcggcttcct ccgcgacggg aggaagcctg aggagattaa tcgtgagcgc
    10561 cgatttcgcg cagtttacga cctttcatca gattacgttc aatatgttcc agcgaaacgt
    10621 gtttggtttc cggtaccagc cacaatgtca gcaggataaa cagtacgttc agagccgcat
    10681 acacccagaa ggtgttggcg ttacccagcg tgttgagcat ggtcaggaac gttgcgccaa
    10741 cgatcatgtt ggcaatccag ttggtggcgg tggagcaggt gatgccaaaa tcgcggcctt
    10801 tcagcggctg aatttcggag cacagtaccc aaatcagcgg accggcactc atggcaaaac
    10861 cgacaataaa catcagcagc atggcgatgg cgaaatactg cgccgacgga gagtggatac
    10921 cgatatgcat cattgtaccg agtacgccca tgccagcagc catcaccagg aagcccagcg
    10981 ttagcgttgg tttacgtccc cagcggtcaa caaggccgat tgcgataaag gtggcaagta
    11041 cgttggtcag gccgacaatc actgtccccc acatttgctc ggtggtgttg gtataacccg
    11101 ccagttcgaa gattttcggc gcgtaataca tgatgacgtt catcccggtg aattgctgca
    11161 ttacctgtaa cagtacgcca aggaacaccg cgcggcggaa gttgctattc tctttaaaca
    11221 gcgcccagcc actctgttta acctgcaaac tttcacggat ttcatccagt tcgcgtttcg
    11281 cttccgcgct ggtgtcacgc aggcgtagca gcacgcgttc ggcatcaaca aaacggcgtt
    11341 tggcggcaaa ccaacgtggg ctgtctggca ggaagaagac accaatcagc agcaaaattg
    11401 ccgggatgat aatcacaccc agcatccagc gccatgcacc ggtgtagctg aaggcggtat
    11461 cagaaagata agcaccgagg atcccgatag tgatcatcaa ctgatacatc gagatcatac
    11521 tgccgcgaat tttttccggc gcaatttctg acaggtacag tggtgcggta taagaggcca
    11581 cacccaccgc cagccccagc agaacgcgcg aaagaatcag tacttcaacg tttggcgcag
    11641 ccgcagagaa cagtgaaccg gcaacaaaca ggattgcgcc gatcatcagg ctctttttgc
    11701 gcccgagttt aaaggagagc cagccgatgc ccaccgcacc gactgccgca ccgaacatca
    11761 tggagcttac gacccattct tgcgtgtgcg aagttatctg gaattcatct gcaataaacg
    11821 gcagtgcgcc agcaattaca ccgatatcca ggccaaagag taatcccgcc agagcggcaa
    11881 ggaagcagac gaaaaacgtc attgccttgt ttgaccgccc ctgtttttta gcgtcaggca
    11941 tgatgccctc caatatagtt attttttatt gtgaattaag ataggtgagt acgacgtaaa
    12001 aagatgtgaa gcaaatcaca tcagtgtaat cgcttacact aacatttatc ttag
//
LOCUS       DAZOYF010000120        12021 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZOYF010000120 DAZOYF010000000
VERSION     DAZOYF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.120"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(33..2282)
                     /locus_tag="ACHUOF_003043"
                     /pseudo
     CDS             complement(33..2282)
                     /locus_tag="ACHUOF_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
     gene            complement(2306..2929)
                     /locus_tag="ACHUOF_003044"
     CDS             complement(2306..2929)
                     /locus_tag="ACHUOF_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFR0612115.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2926..4614)
                     /locus_tag="ACHUOF_003045"
                     /pseudo
     CDS             complement(2926..4614)
                     /locus_tag="ACHUOF_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4763..7390)
                     /gene="gyrA"
                     /locus_tag="ACHUOF_003046"
     CDS             complement(4763..7390)
                     /gene="gyrA"
                     /locus_tag="ACHUOF_003046"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR0612116.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7537..8259
                     /gene="ubiG"
                     /locus_tag="ACHUOF_003047"
     CDS             7537..8259
                     /gene="ubiG"
                     /locus_tag="ACHUOF_003047"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR0612117.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8387..11950)
                     /gene="yfaL"
                     /locus_tag="ACHUOF_003048"
                     /pseudo
     CDS             complement(8387..11950)
                     /gene="yfaL"
                     /locus_tag="ACHUOF_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2784 a         3329 c         2962 g         2946 t
ORIGIN      
        1 caggtggcca aattcagtgt gccacttcat cattcgacat tgaccagttc accaggctgg
       61 taatgggttt tgtccgtttt cacccggata tcaagctgag gaacggcaac tttgatcccg
      121 gcgttctgaa aactgtactg accattacgg gtatacagca ccgaaaaagt gatgttaggc
      181 gcaaaggaat tgctcactgg tacccgggct tcatacaggg tatcgtttaa acgttgtagc
      241 gttagccagt ttgccggatg cgaaagcagc gactgctgtt ccacgcgatc gcgttccagc
      301 gtcaataacg cttcatcaat tggctccggg aaagtaatca gcatcttcgc ggtttcgcct
      361 ggctggtaca gcgttttatc cgccacgata tctaccgtac cagtatgcgc cgtgctgccc
      421 ttaccgctga cggcatgact taacccagcg agaattaagc cgtctttatc gcgtaatgtc
      481 agattgtagt tgccaggttt agcgaaattg acggtaaagg atttgccgcc tgacggtagc
      541 tctccgctat ggctcgtgcg gtcttcgaga cgcaaccatt catacgtaac aggaacctgt
      601 tttgaagatt ccagcgcggc ataacggaac acaaccgact cgccgctatt actgtattgt
      661 gcggcggtac ttaaagagta atgcgccagg ccgcgttcaa tgaggatctc tttggtggtg
      721 gtgacgcgat acgccgcgcc gtcactggcg gagacggtta acaaatagcg gctcggttta
      781 tcggcggcgg ggagatttaa cgccacatga ccgctggcgt cggacaccgt ttcgctgcct
      841 tccagcgaca cggggaaacg tccggcataa cgcaaatcgt taccgaccat tgataattgc
      901 tgagcgcgca aacttaactg cacgcgggca ttttttaccg gctcgccatc cgggtagagc
      961 agttgcagtt tgccgctgac cgcttcgcca gttttgaact cttttttgtc gagagctaaa
     1021 ccaatctcga aatgtggctt gatgtagttt gccacgcgaa aactgctgct atagacctga
     1081 ttgcggtaag caagacgtaa ctcataacct ccggcaacgg cattttctgg caggcggaaa
     1141 cttccctgcc cgccattgcg cgcatccagc gtgacatcga cggtttgcaa cagactgccg
     1201 ttagcgtcca gcaccgaaag cttcgccggg gcgctgacga tgggggatga atgcaacgga
     1261 tcgcggaact cgcggcccat cactttaaca tcgacacgat cgcctgcgcg atatagcggg
     1321 cgatcggtaa aaatatacaa gcgggtgttg tagatttcgc tttcgtagaa gaagttctcg
     1381 gagacaaaaa cgccgccttc agcatcctta cccagaatgt atgaacgttc tggcgatata
     1441 tgttgtaact gcaaagtacc gctgtcatcg gtcaccccgc gggtcatcac gccaagacct
     1501 tcagtccaca agatctctga gccgggcttc gcttcaccct gttttttacc cgcggtccat
     1561 accagaagct cgttacctga cactttgcta agcgccacgg tatcggaaac aaacaccacc
     1621 gtcgtcgccc gatacccacc taccatcgcc tcgacgaggt acagccccgg ctcttgttgg
     1681 ccgagaggaa tataaatatt acccggctgc ggcgagatga aattgctgga tgcgccttcc
     1741 agttttaccc cttgctgcgg ctcgaccggt ttagcctgcc atagtggata acggaactgt
     1801 tccaccaagg gatatttttt cagcggggaa aactggttgt tctgtacata acgggaaggt
     1861 ttaataatgg cattgccgag atgtaattcg ggtaatgcct gagtcacatt ctgccgtgac
     1921 tgagaagaga aagtacgctg catcacgcgg cgagatttgc cgtaccagtt atcccacagc
     1981 caggtcagcg tattgttcag cccgtcgccc agatattgcg gttgcaccac aatgcgatgc
     2041 aggtttttct gctggcgcaa aaatgccatc gggtcaggaa tacgatatag gcgaacgtcc
     2101 acgccgccgt actcttccat ctgatagcgc cgataatcac gccccggcgc ttccagtcgc
     2161 actttcgcct cttcactgct gctaaaactg ctgtcagcaa gcaaaaagaa tgttcccccg
     2221 gcgggcggcg catagttact ggaaggaagc gaatcatcag ctttagcaag ccctgttccc
     2281 acaagagaca gggacaagca cgctgctatc gcgccagaaa atttaaacga taaatgccaa
     2341 tgaaattggg attggaatcg ttgggtatcc atcgggtgtc cttccatgtc ataagttgtt
     2401 gcagactgac tgcgcgcatt ccgttgtcag ttttcgtggc gcttccggtg tggtagatga
     2461 cgtaacgccc catccagacc attaagtgct gggcatcgcc ctgatcgaaa aaaatcatat
     2521 cgccaggcag cgcctggttt atgtcttggc caataaactg gctgttgtac tgaatcaaat
     2581 taatcgcggt cacgtagggg ccgtttttcc cgctcccctg attccagttt tgcgccagtt
     2641 gacgctgttc aggtgttagc gtcatctctg gcggcaaata ctggctagat aaaccgttac
     2701 tttttagcca tttactgtcg tgaactttca gcgtctcgtt cgccgcaaat cgcaccaggc
     2761 ccgcacaatc ctgctgatac cagcgtggac ttggcccctg gcggagctgt tcttgtgcaa
     2821 tgcgtacaaa ccaggcgcga aacagcctgg attgttcgac attcagcatt tcgctatggg
     2881 caacaacaca acacagccag caaatcagcg ccagcagccc gtgtctcata gcggctgcca
     2941 ggttatgggt agccactgcc aggcgacacc gggttccatc tgggccagct tcatgacata
     3001 acgcggttgt tgagataaag cgtccagctt cggcattaat aaagtttgtg cggcgttata
     3061 aaaaaccggt tcgagattct tcggcagact ggtcaacgtt tcgttacgca gcagtttcgc
     3121 cacgccttgt ggattgatat agagcggaac gatgccatca gtgggtatta catccaccat
     3181 tgccgggcgg gttttattca gtgtttgcag ggcgttatta accagcgtgt catcgaggga
     3241 gaaaagcagc gttttgtttt gcatcgccag cgacacacgg aaaaaataat ccgacattaa
     3301 ttgatcgggt tgcgccgcct gcgctttcgg atactgaccg tatcgggaac tcacttcgcg
     3361 acaccagatt tgcgcttcgc cctgctgagt ctggcttacc ggcaaaacac cttctggcgc
     3421 tttgctttcg tgcgcaccaa tattttgcgt aaacagtttc cctggcaatt gcgcctgttc
     3481 ggcagtgcca tcaaactgac cgacaaacag cggggtttgc aattttgagt cttcatacca
     3541 gcacagcccc gcggcaccgt ctaacgcccc attcagcttg tcgttttcct ggctgatgtg
     3601 cgaaagcatc tcttcggcaa taccgtgtga atacggcacc gccacacaga agctggctcc
     3661 ggcaggcata ctgttccaga ccggcgtaaa atcgaaactg gcatctacgc tggcggattc
     3721 atcatttaac gccacaaaac tgtgccagcc gtcgttaccc atttcgaagc gtacgccagc
     3781 aaaagaaggc attaaccgct ggtagccaaa acccagcaac ctggcgctga ccacgatgcg
     3841 caggcgtact ggcgtttttt cggctgtacg ctcttccagg ccaaagctgg cttgccagcg
     3901 ttttttaccg ctcaacaaat cacttgcgat cgccgtggct tcggtatcct gctgatcatc
     3961 tttaaacaac atatccgtac tggaaaacac cagcatcttg tcctgatatg tcgcgaacat
     4021 cagggcgtta ttgccgttat agcgcaactg ataaacagga atagtttcac tatttatctt
     4081 gatgctactg atttccgttt tgcttaacta gctgtcgctg gtagcggcaa acaacaatgg
     4141 ctccagcagt ttgcttaaac cactgcgctg gatcagcacc atgtaatgtg aaagatggcc
     4201 ttgtttatcg tgccatagcg ccgcctgcgc gggctgatct aagagtgacg aaaagagctt
     4261 atctttcagc gtgagatcgt gttcatagac aatgcggcga atgctgcctt caatgcccag
     4321 acgatcggca tgattctgat aatagaaaac gaaatcttcg ctcagaacat cgtggagaaa
     4381 cggaatggtg aggagatctt tgggaagctg gctcagagag tcgctgtcta gaaagaggtc
     4441 cggctcattg agatcgattt gcagattgtt gtgcaccacc agcggcgaca acgttttttc
     4501 tggcccactg ccagcatatt gcaacgccca gacgccagcg gaaagcagtg ctattgcgcc
     4561 aaaacctaca agaccataga accgccagcc tttcgccttt ttttcaccac tcattgccac
     4621 attccttgtg tatagccagc cattttttac gggcacagcc aaactttacc gcgccctaat
     4681 acgacaaaag cccagacttt gcagcctgga cttttcaatt caaacaaggg agatagctcc
     4741 cttttggcat gaagaagtaa aattattctt cttctggctc gtcgtcaacg tccacttccg
     4801 gagcaatttc atcatcccct tccgcggcac tgccgtcgat ggtatccaga tcttcctcgt
     4861 caaccggttc agcaacacgc tgcagaccca ctacgttttc atcttccgca gtacggatga
     4921 ggatcacgcc ctgggtgtta cggcccacga tgctgatttc tgaaacgcga gtacgtacca
     4981 gcgtaccggc atcggtgatc atcatgatct ggtcgcagtc atctacctgc accgcgccaa
     5041 ccactaaacc gttacgttcg gtaaccttga tggagataac ccctttcgtc gcacgcgact
     5101 tggttgggta ttccgccact gcagtacgtt taccgtaacc gttttgcgtt gcggtgagga
     5161 ttgcgccatc gccacgaggc acgatcagag agacgacttt atcgccttca cctaagcgaa
     5221 taccgcgcac accagtggta ttgcagccca tcgcacggac agaagactct ttaaagcgca
     5281 ccactttacc ttcggcggag aacagcatta cttcatcttc gccactggtc agatcaacgc
     5341 cgatcagctc atcgccgtca accagtttga tcgccacttt accggcggta cgcagacggt
     5401 tgaattcggt gaggacggtt ttcttcacag taccgttagc ggtcgccatg aagactttca
     5461 cgccttcttc aaactcggtc accggcagga tcgcggtgat acgttcgtcc tgttccagcg
     5521 gtagcaggtt gacgatcgga cgaccgcgcg cgccacgagt ggcttccggc aactggtaaa
     5581 ctttcatcga atagacgcga ccacggctgg agaagcacag aatatggtcg tgcgtgttcg
     5641 ccaccagcag tcggtcgata aagtcttctt ctttaatacg tgcggcagat ttacctttcc
     5701 cgccacgacg ctgcgcttcg tattcagaaa gcggctgata tttaacgtag ccctggtgag
     5761 agagcgtcac gaccacatct tcctgggtga tcagatcttc caggttgatg tctgcgctgt
     5821 tggcggtgat ttcagtacga cgtttgtcac cgaactgttc acgaaccagc tccagctctt
     5881 cgcggatcac ttccatcaga cgatcggcgc taccaagaat acgcaacagt tccgcgatct
     5941 gatccagcag ctctttgtat tcgtcgagca gtttttcgtg ctcaaggccg gtcagtttct
     6001 gcaaacgcag atccagaatc gcctgagctt gctgttcggt caggtagtac agaccatcac
     6061 gcacgccgaa ctctggctcc agccattccg gacgcgcagc atcgtcgcca gcacgttcca
     6121 acatggcggc aacgttgccc agctgccacg gattagcaac cagcgcagtt ttcgcttctg
     6181 caggcgtcgg cgcatgacgg atcagttcga tgatcgggtc aatgttcgcc agcgccacgg
     6241 ctaatgcttc aaggatatga gcacgatcgc gagctttacg cagttcgaaa atagtacgac
     6301 gggtcaccac ttcacggcgg tgacgaacaa acgccgcgat gatgtctttc aggttcatga
     6361 tcttcggctg accatggtgc aatgccacca tgttgatacc gaaagaaacc tgcaactggg
     6421 tctgggagta gaggttgttg agcacaactt caccgaccgc atcgcgtttc acttcaatca
     6481 cgatgcgcat accgtctttg tcagactcgt cacgcagcgc gctgatgcct tccacgcgtt
     6541 tttcttttac cagttccgca atcttctcga tcaggcgcgc tttgtttacc tgatacggaa
     6601 tttcgtggac gataatggtt tcacgtccgg ttttggcgtc aacttccact tcagcacggg
     6661 cgcggatata taccttgccg cgaccggtac ggtaagcttc ttcaataccg cgacgaccgt
     6721 taatgattgc cgccgtcggg aagtccggcc ccgggatgtg ttccatcagc ccttcaatgc
     6781 tgatgtcttc atcatcgata tacgccagac aaccgttgat gacttccgtc aggttgtgcg
     6841 gcgggatgtt ggttgccata cctacggcga taccggaaga accgttcacc agcaggttag
     6901 gaattttggt tggcatgacg tcgggaattt tttccgtgcc gtcatagtta tcaacgaaat
     6961 cgaccgtctc tttttcgaga tcggccatca gttcatgggc aattttcgcc agacggattt
     7021 ccgtataacg cattgccgcc gcagagtcgc cgtcgatgga accgaagtta ccctgaccgt
     7081 ctaccagcat gtaacgcagc gagaatggct gcgccatacg gacgatcgtg tcataaaccg
     7141 ccgagtcacc atggggatgg tatttaccga ttacgtcacc aacgacacgg gcagattttt
     7201 tataggcttt gttccagtca ttgcctagta cgttcatggc gtaaagtacg cgacggtgta
     7261 ccggcttcag gccatctcgg acatctggca gcgcacggcc aacaatgacc gacatcgcat
     7321 aatccagata ggagctcttt agctcttcct caatgttgac cggtgtaatt tctctcgcaa
     7381 ggtcgctcat ctaaccgcta tccctctact gtatcccgga ttcaaaggtc gcaaattata
     7441 acacagccgc gcagtttgag gtaaacctat acgctttatt cacatccaat gcctgatata
     7501 ctcgtttgtc ttgccaatta cggagtagaa gtgccaatga atgccgaaaa atcgccggaa
     7561 aaccataacg tagaccacga agagatcgct aaatttgaag ccgtcgcctc ccgctggtgg
     7621 gatcttgaag gtgagttcaa accgttgcac cgcattaacc cgctgcgtct gggctatatt
     7681 gccgagcgtg ctggcggttt atttggcaaa aaggtgctcg atgtcggttg tggcggcggc
     7741 attctggccg agagtatggc gcgcgaaggc gcgacggtga ccggtctgga tatgggcttt
     7801 gagccattgc aggtggcaaa gctgcacgcg ctggaaagcg gcattcaggt ggattacgtg
     7861 caggaaaccg tggaagagca cgcggcaaaa catgccgggc agtatgatgt ggtgacctgc
     7921 atggagatgc tggagcacgt acccgatccg cagtcagtgg tcagagcctg tgcgcaactg
     7981 gtgaaaccag gcggcgatgt ctttttctcg acgcttaacc gcaacggcaa gtcatggctg
     8041 atggcggtgg ttggcgcgga atatattttg cgcatggtgc ccaaaggtac gcatgatgtg
     8101 aagaagttta ttaaaccggc agagctgctt ggctgggtgg atcagaccag tttaaaagag
     8161 cgacatataa ccgggctaca ttacaacccg atcactaatt cctttaagct cggccccggc
     8221 gtggatgtga actatatgct gcacacgcag aataagtgag gttgatgttt ggccgcgcca
     8281 atgcctgatg cgacgcttgc agcgtcttat caggcctaca aatgctctcc gtaggccaga
     8341 taaggcgttt acgccgcatc cggcaattgt gatggtcaga cagcgattac catttcaccg
     8401 tcatcgacaa aaaccctgcc gtctgggcaa aatcatcact ccctttctgc cacgccacgc
     8461 tgccgcgcag ggacactcgc tgactgatat tgcccgtgac tcccactttt atttcacccc
     8521 gtagcttcac cgcatcgtca ctgatagtgc tgccatcttc ttcaatttcc gtcgcatggg
     8581 gatcgtgata gtaattcaga tctaatgttg gtgtgacacc aaccgcggta cgccattcgc
     8641 tgtgtaaacc cagccgcgtc tgaatatcat ccccctgcga ttgtgacacg cgcgcatggt
     8701 tagcggcggt aaaatcatcc tgctgcacgc cctgataaat cacctgcgcc tgcggttcaa
     8761 tcaccacacc acgccccggt aaccactgat acccagcttc cagcgaggcg ataatccccg
     8821 acgaatggta acgatccacg ccatcttcat gttcggaaac atcattgtta aaccacgcgt
     8881 attgcagcca ggccccctgc ttctgattac cgtgctgata ccagcttgag gtcagcccaa
     8941 cggcataacc gtggttctgg ttatcggcgc gagttccggt catattcgag cggctgtcgc
     9001 cctgattatc actgtagcca ccaaccgccc caagcatcca ctcgccatcc gtgccccagc
     9061 gcccgctaaa caagccgccg ctaagctgca ccgtagaagt gtcttcctgc tgagccagtt
     9121 gccctgccgc cgtgtaatga taacgtccgc cgataacacg taaattcagc gtctgaccat
     9181 cgccaccagc gtgatcgcgt cgctccatca taaacgcctg atttgccgcc cgcaggttat
     9241 tgagataacc gccaactttg gcattcagca ccggctggta agcaggcgta ggggccgggt
     9301 cgggggttgg gtcaggatcc ggcgtgggat caggatcggg agtcgggtct ggctcaggtg
     9361 gcgatggcgg cgttacttct tgcgatcgca gataccagtc gttgttatct tccactagcg
     9421 tgtagtcata cgctcccatg ttgacgtagc cactgccagc cagactgaac tgtgcattgt
     9481 tttgaaactg cgtgggatca gctgcgaaat caaccacttt aatgcctgtc gatgtcggct
     9541 caccaatccc tgtaatggag ttaaccacca cagttgtgtt gccagcagta ttaccgttca
     9601 tcaccaattg atcgcttacc gagtcatcgc cgtttaattc gctgtcgaat agtagcgtac
     9661 cgccgccggt ataatcaccg tttaccgtca gcgtatcgcc agcgacgcca ttttgcaggc
     9721 taaccgcacc ggacgtattg gtcagatcac cattaaccgt cacgtcagca agataggtca
     9781 ggctgtcagt ttcaaggttg gtattaagct cgcctgtcaa cgtcacgcca tcggtagcat
     9841 ttaccagcgt cccatcgccg ttaaatagtg aggcattaag ggcgacggaa gtatcctgcc
     9901 cggtcagacg caaaaccgta ccatcgctga tgtcgatagt gccgctggaa gtagcatcaa
     9961 ttgactgaat atcctgatcc gcgccagtaa cgaacgttgc cccatcacca acccacagtg
    10021 acgaagcata aggaaagata tccgcaacat cgcctttcag ggtgccttct tcgacacgta
    10081 ccgccgactg cgttgtaccg ctggcggtca gctccagcgt acccgccccc gttttagtaa
    10141 atgtgctacc ctcatcctga tgctgcccgc tgctgtcagc catcagtgcg ccccactgcg
    10201 tgtctacccc cgcatcaact gccacttcac cgtcggcgcg catttcaata tcacgcccat
    10261 gctcagtcgt cgtagagcga gtatcgacat tggcagtaat ctccatcacg ctttcttgtt
    10321 acttatcggt aaagataacc tggcggttat agtgggtatc tccaagctgc gagttgtcgc
    10381 tcaccatcag cgtaccggag gcgatttgtg ttgtgcccaa ataactatta ttgtttgcca
    10441 gacttacctg cccgtcgctc gcatcgacca ccacgtagac gccatctttc ccgtcgccgc
    10501 cgatatgcaa attgtcgcca cctgcaagat ctaccacatc ctgactgccg ataaccgttc
    10561 cgctgctgcc actgacttca aggccatcat tgtatgatgt gccgctctgc caagtggaga
    10621 aatcagagag atcgagcaca ccgccgttta acacgatcga ctgcggatcg tcctggagag
    10681 cggtaagatc tgccgcgtcg ccttccagcg aaagcaccgc accatcatca acgactatat
    10741 cgccggttag cgccatcgac tgcgcccctg ccagcacgta gctgccgttt tgcgcaatgg
    10801 tgagctgacc agcaccttcg atgatgccag caaaactgcc ctgattaaca gtaacgttgc
    10861 caccagcatc gatatttaaa gtgccattct gaaagcccgt caatgcgtgc acaaaagttt
    10921 gttgggtcga gccaacgttt agctcagcct gattctgata ctgatcaata ctccctatcg
    10981 tcagaccgta gcagtcttgc ggatcgtcct ggcaatgcgt atcgccgaca ttcatcaggg
    11041 agttgctgcg acccagggta acttcaccgt tttcaatctg catctcacca gtaaagtcat
    11101 tgttatctgc attaagtacc agatcgccgg aacctgtttt ggtgattaac ccggtaccag
    11161 caatagagtc aacagctccg tcattctctg tattgccgat aaccagcgtt tttccgtcgg
    11221 caatatcaaa ggtaacttca cttaagccga gatacataaa gccacccgcc gcagtggagg
    11281 gaccatctcc atagcctgct gcgctattgt tctcatcgac taacacgccg ccgttctggc
    11341 tgtagctgtc atcaacagaa atatcaataa gatagggagc cgtcgcgcta ttggtatata
    11401 tcgccccgcc ataaccttcg gctgtgttat ttgtaaaggc ggtgttattt attatcgtat
    11461 aacctgaagg atgcttgctg tcgctattat tatcggtaac atcgattgcc ccgccatcgc
    11521 catcactgta acttgttgat gtatatgcct ggttattgtt aaaaacatca tcacttaaat
    11581 agacgtcatt attaatggta taaattgcgc cacctttgcc atcattagcg atattattga
    11641 tggcgttagt gacacgtaaa tcgacggcac cagtatcatt agtaccagaa gaatagattg
    11701 cgccaccata gccgcctgcg acgttaccgg aaaaaataac atcagtaaga ttcagcgttg
    11761 agttttcttt agcaaatatg gccccgccgt tattatattc tcctgtaacg gtgttattgg
    11821 caaacagagt cattccagtt tcattttctg gtaatagtga aaattccgct ccttgttgca
    11881 aaaatacggc cccaccgctg gcattattgg tcatatccga aaaaaccagc cattgcccat
    11941 cggcgataga ctggccccct gaatctccag acaaccagta tcacttattt aagtgatagt
    12001 cttaatacta gtttttagac t
//
LOCUS       DAZOYF010000121        11891 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZOYF010000121 DAZOYF010000000
VERSION     DAZOYF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.122"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(126..866)
                     /gene="cysQ"
                     /locus_tag="ACHUOF_003049"
     CDS             complement(126..866)
                     /gene="cysQ"
                     /locus_tag="ACHUOF_003049"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR0612118.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            1056..2999
                     /gene="cpdB"
                     /locus_tag="ACHUOF_003050"
     CDS             1056..2999
                     /gene="cpdB"
                     /locus_tag="ACHUOF_003050"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR0612119.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3125..3505)
                     /locus_tag="ACHUOF_003051"
     CDS             complement(3125..3505)
                     /locus_tag="ACHUOF_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR0612120.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3594..4454
                     /gene="qorB"
                     /locus_tag="ACHUOF_003052"
     CDS             3594..4454
                     /gene="qorB"
                     /locus_tag="ACHUOF_003052"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0612121.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            4563..5528
                     /locus_tag="ACHUOF_003053"
     CDS             4563..5528
                     /locus_tag="ACHUOF_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0612122.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5636..6298
                     /gene="ytfE"
                     /locus_tag="ACHUOF_003054"
     CDS             5636..6298
                     /gene="ytfE"
                     /locus_tag="ACHUOF_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR0612123.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6343..7755)
                     /gene="cycA"
                     /locus_tag="ACHUOF_003055"
                     /gene_synonym="dagA"
     CDS             complement(6343..7755)
                     /gene="cycA"
                     /locus_tag="ACHUOF_003055"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR0612124.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(8063..8683)
                     /gene="fklB"
                     /locus_tag="ACHUOF_003056"
     CDS             complement(8063..8683)
                     /gene="fklB"
                     /locus_tag="ACHUOF_003056"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0612125.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8902..9447
                     /locus_tag="ACHUOF_003057"
     CDS             8902..9447
                     /locus_tag="ACHUOF_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HFR0612126.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(9431..10078)
                     /locus_tag="ACHUOF_003058"
     CDS             complement(9431..10078)
                     /locus_tag="ACHUOF_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0612127.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            10290..11081
                     /locus_tag="ACHUOF_003059"
     CDS             10290..11081
                     /locus_tag="ACHUOF_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFR0612128.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            11091..>11891
                     /locus_tag="ACHUOF_003060"
     CDS             11091..>11891
                     /locus_tag="ACHUOF_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR0612129.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINS"
BASE COUNT         3007 a         3083 c         2992 g         2809 t
ORIGIN      
        1 aaatgtaact tactagcaat tgaatttttg cactttaaat acaatctctt tttatttata
       61 actttttagg ttgggataaa tcaggatggc agaatcagga aatacactgt ttctgccatc
      121 tgaatttagt aaatggacac tctgaacccc ggattcagga acgactcgcg cggagtgtaa
      181 tccagcggtt taccctgcca gtcgtgaacg tgcgctccgg cagctgcagc cacagcatgt
      241 ccggcggcgg tgtcccaaat attcgttggt ccgaagcgcg ggtacagctg cgcctgtcct
      301 tccgccacca ggcagaattt cagcgaagag ccgatggacg tggtctgatg ttcgccaagc
      361 tgttgcagat actctttcag ctccgcatct gcatgggaac ggctgataac caccagcggc
      421 gggcgcgcat cgcggacctg aatcagcttg cgcacaccgc actcttcttt ccaggccttg
      481 ccttctgccg cgctgtacat tacgttcatt accggcgcat acaccacacc taaaatcggt
      541 ttgccatggt caatgagcgc aatgttaacg gtgaattcgc cattacgttt aataaactct
      601 ttagtaccat ccagcgggtc taccagccag taacgctgcc agtgctgacg gacttcccaa
      661 ccgggaggat cttcttcaga aaggaccgga atatccggtg ccagcgtacg taaaccgtcc
      721 atgataacgg tgtgagcggc aatatccgct gccgttaccg gagaattgtc cgctttgctg
      781 acgacgtcca tcggtttcgt cccgtcgtag acctgcataa tggcatcgcc tgcattccgt
      841 gcaagctggc atacttgatc taacatttct ccacctcgtc tctgtgagcg gtgttaactt
      901 attgttttac ttatacccta tcgttaatga atgcgccaac tgtgatagtg tcatcatttt
      961 caaagcgtaa aattgtggca ttcttcactg ttctataagt aaggcgttta ttctcccttt
     1021 tctttcgtat tcccgatgat aaaaggatgt ccctgatgat taagtttagc gcaacgctcc
     1081 tggccacgct gattgccgcc agtgtgaatg cagcgacggt cgatctgcgt atcatggaaa
     1141 ccactgatct gcatagcaac atgatggatt tcgattatta caaagacacc gccacggaaa
     1201 aattcggact ggtacgtacg gcaagcctga ttaacgatgc ccgcaatgaa gtgaaaaaca
     1261 gcgtactggt cgataacggc gatttgattc aggggagtcc gctggccgat tacatgtcgg
     1321 cgaaaggatt aaaagcaggt gatgttcatc cggtttataa ggcgctgaat acgctggatt
     1381 atacggtcgg tacactcggc aatcatgaat ttaactacgg tctggattac ctgaaaaatg
     1441 cgctggcggg agcgaaattc ccttatgtaa atgccaacgt cattgacgcc agaaccaaac
     1501 agccaatgtt tacaccgtat ttaattaaag ataccgaagt ggtcgataaa gacggaaaaa
     1561 aacagacgct gaagattggc tatattggcg tcgtgccgcc gcaaatcatg ggctgggata
     1621 aagctaattt atccggaaaa gtgacggtga atgatattac cgaaaccgtg cgcaaatacg
     1681 tgcctgaaat gcgcgagaaa ggtgccgatg tcattgtcgt tctggcgcat tccgggctgt
     1741 ctgccgatcc gtataaagta atggcggaaa actcagttta ttacctcagt gaaattccgg
     1801 gcgttaacgc cattatgttt ggccatgctc acgccgtttt cccgggtaaa gattttgctg
     1861 atatcgaagg ggctgatatc accaaaggca cgctgaatgg tgttccggcg gtaatgccgg
     1921 gcatgtgggg cgatcatctt ggggtggtcg acttacaact cagtaatgac agcggtaaat
     1981 ggcaggtgac gcaggcgaaa gcggaagcac ggccgattta cgacatcgcc aataaaaaat
     2041 ccctcgcggc ggaagacagc aagctggtag aaacactcaa agccgatcac gatgccacac
     2101 gccagttcgt cagcaagcca atcggtaaat ctgccgacaa tatgtatagc tatctggcgc
     2161 tggtgcagga cgatccgacc gtgcaagtcg tgaacaacgc gcaaaaagcg tatgtcgaac
     2221 attacattca gggcgatccg gatctggcaa aactgccggt gctttcagct gccgcaccgt
     2281 ttaaagttgg tggtcgcaaa aatgacccgg caagctatgt ggaggtggaa aaaggccagt
     2341 tgaccttccg taatgccgcc gatctttatc tctaccccaa tacgctgatt gtagtgaaag
     2401 ccagcggtaa agaggtgaaa gagtggctgg agtgctccgc cggacagttt aaccagattg
     2461 ctcctaacag cacgaaacca cagtcactca tcaactggga tggtttccgc acttataact
     2521 ttgatgttat tgatggtgtg aattatcaga ttgatgttac ccaacccgcc cgctatgacg
     2581 gcgagtgcca gatgattaat gccaatgcag agcggattaa gaacctgacc tttaatggca
     2641 agccgattga tccgaacgct atgttcctgg ttgccaccaa taactatcgc gcttacggcg
     2701 gcaaatttgc cggtacgggc gacagccata tcgcttttgc ttcaccggat gagaaccgct
     2761 cggtgctggc agcgtggatt gctgatgagt cgaaacgtgc gggggaaatt catccggcgg
     2821 cagataacaa ctggcgttta gcaccgatag ctgccgataa gaaactggat atccgtttcg
     2881 aaacttcccc gtcagataaa gccgcagcgt ttattaaaga gaaagggcag tatccgatga
     2941 ataaagtcgc gaccgatgat atcgggtttg cgatttatca ggtggatttg agtaagtaaa
     3001 acacttcttt ttcggcctat aaatcaaccg catccggcat ttattggcgg atgcgatgct
     3061 gccgtatctt atccacccta caagccatga accgtagacc ggataagcgc agcgcatccg
     3121 gcagttatgc cgcacgttca tcccgcaccg ccaacacctc gggcaaattc aactcaatcc
     3181 agtccgccag tgcagcaacc ttttcgctca cctgctcgcc cagcggcgtg aggctatatt
     3241 ccacatgcgg cggcaccacc ggatacgcga tacggttaag aaaaccatcc tgttctaacg
     3301 cctgtaacga ctgcgcaagc atcttttcac tcaccccacc gattttgcgc cgcaggtcgc
     3361 taaagcgatg agtaccttcg cgtagcgcca ccagaatcaa caccccccaa cggctggtga
     3421 cgtgtttcaa cacctcgcgc gacgggcact gttccgcaaa gagattaccc tctttcagtt
     3481 gttgtgacag gctaacctga ctcatttcat acttaccttt ttgcacgtac ttactaaaag
     3541 taagtttagg tgttagcgta tttaaacaca agacaaaacg atggagactt cccatgatcg
     3601 ctattactgg tgccactggc caacttggtc actatattat tgaatccttg atgaaaacgg
     3661 ttcctgccag ccaaatagtg gctatcgttc gtaatccggc aaaagcccag gccctggcag
     3721 cacaaggcat taccgtgcgt caggctgact acagcgatga agccgcactg acatctgcac
     3781 ttcagggagt ggaaaaacta ctgctgatct cttccagcga agtgggtcaa cgtgccccgc
     3841 agcatcgtaa tgttattaat gccgcaaagg cggctggcgt gaaatttatc gcttatacca
     3901 gcctgctaca tgcagatacc tccccgctcg gcctcgccga tgagcacatc gagacggaga
     3961 aaatgctggc tgattctggc atcgtttaca ccctgctgcg caacggctgg tacagcgaaa
     4021 actacctcgc cagcgccccg gcagcactgg aacacggcgt atttatcggt gcggctggcg
     4081 atggcaaaat cgcctcagca acgcgggcag attatgcgac agctgcggca cgcgtgatta
     4141 gcgaagccgg tcacgaaggc aaggtttacg aactggcggg cgatagtgcc tggacgttga
     4201 cacagttagc ggcagagctg accaaacaga gcggcaaaca ggttacctat caaaatctga
     4261 gcgaagccga tttcgccgcg gcactgaaaa gcgtcggact gcccgacgga ctggcggata
     4321 tgctggcgga ttctgacgtt ggcgcatcga aaggtggtct gtttgataac agcaaaacgc
     4381 ttagcaaatt gattggctgc ccaacgacaa cgttagccga aagcgtaagc catcttttta
     4441 atgttaataa ctagttaatt aaagtggcat cctcccgcat cctctctgat aatgacggga
     4501 tgccgggagc aatcatgtct gcttcctgaa cgtttcttct gacagaccaa tggatgccag
     4561 taatgattag cggcgtgctg tacgccctgt tagcagggtt gatgtggggg cttatttttg
     4621 tcgggccgtt gatcgtgccg gaatacccgg cgatgttgca gtcgatgggg cgttatctgg
     4681 cgttagggtt aattgcgcta cccattgcct ggctgggacg cgtgcgtctg cgtcagttgg
     4741 cgcgtcggga ctggcttacc gccttgatgc tcactatgat gggcaacctc atttattact
     4801 tctgccttgc cagtgccatt caacgtactg gcgcgcctgt ttccacgatg attatcggca
     4861 ccctgccggt ggtgattcct gtctttgcca atctgcttta tagccagcgc gacggcaaac
     4921 tcgcgtgggg aaaactcgcc ccggcactga tttgtattgg catcggcctg gcgagtgtga
     4981 atattgctga gttaaaccac ggactccccg attttgactg ggcacgttat acctctggca
     5041 tcgtgctagc gttagtttcc gtggtctgct gggcatggta tgccctgcgc aacgcccgct
     5101 ggctgcggga aaatcccgac aaacatccga tgatgtgggc gacggcgcag gcgctggtca
     5161 cgctgccggt ttctctcatc ggctatctcg tcgcctgtta ctggctgaat atacaaacgc
     5221 cggacttctc cttacctttt ggcccccgtc cgctggtgtt tattagtctg atggttgcga
     5281 tagccgtgct ttgctcatgg gttggcgcac tctgctggaa cgtcgccagc cagcgattac
     5341 cgacagtgat tctcgggccg ctgattgttt tcgaaacgct ggcaggtttg ctgtacacct
     5401 ttttactccg ccagcaaatg ccgccgctaa tgacgctgag cggtatcgcg ctgttagtga
     5461 ttggcgtggt catcgcggtc agagcaaaac cagaaaaacc tttaactgaa tctgtctcag
     5521 aaagttgaca tgctggcagt gagttaaata agcctctgct acgtaagggt tatagctttt
     5581 gccttaaagg tgtatttaaa atacatctta tcttattaag aatgaggtat cagctatggc
     5641 ttatcgcgac caacctttag gtgaactggc gctctctatc cctcgcgctt cggctctgtt
     5701 tcgtaaatat gatatggatt actgctgtgg cggtaagcag acgctggcgc gcgcggcggc
     5761 acgtaaagaa ctggatgttg aggtcattga agctgaactg gcaaagcttg ctgaacaacc
     5821 gattgagaaa gactggcgta gcgccccgct ggcagaaatc attgaccata tcatcgtgcg
     5881 ctaccacgat cgtcaccgcg agcagctgcc ggagctgatc ctgcaagcga ctaaagtcga
     5941 gcgcgttcac gccgacaaac cgagcgtgcc aaaagggctg acaaaatacc tgaccatgct
     6001 gcatgaagag ctttccagcc acatgatgaa agaagagcag atcctcttcc cgatgatcaa
     6061 acaaggcatg ggcagccagg caatggggcc aatcagcgta atggaaagcg agcacgatga
     6121 agcgggcgaa ctgctggaag tgatcaaaca cactaccaat aacgtcacac cgccgccaga
     6181 agcctgcacc acctggaaag cgatgtataa cggcattaat gaactgattg atgacctgat
     6241 ggatcacatc agtctggaaa acaatgtact gttcccacgc gccctagcgg gtgagtgatc
     6301 taaaagctgg atggcattgc gccatccagc atgataatgc ggttatttcc gcagttcagc
     6361 agcccgcttc ttaccaataa acagccagcc caaccccagc gcgataaacc acagcggggt
     6421 aaccagcagc gcctggcgag tgtcatcttc cagtgtcagc aacacgacca cgaacacaaa
     6481 gaacgccata catacccagc acatcagctt gccgagcggc atcttgtaga tcgacttttc
     6541 atgcagatga ggacgctgtt tgcggtacac caggtaagag caaaggataa tcgtccagac
     6601 gaacataaac agaatcgcgg aaacggttgt aatcatcgtg aacgcgccaa tcacgctagg
     6661 attcacatac aacatcacca cgccgccgag cagacagata cacgagaagg tcaacccttt
     6721 cgcgggtact gcgcgcttag aaagtttagc gaacgctttc ggcgcaacac cttcctgcgc
     6781 cagaccaaac agcatacggc tggtagagaa gacaccgctg ttagcggaag acgccgcaga
     6841 ggtcagcacc acaaagttga tcacgctggc agccgcaggc aaacctacca acacaaacag
     6901 ctcaacgaac gggcttttct ccgggactac cgaactccac ggtgtcacgg acataatcac
     6961 aatcagcgcg aagacgtaga acataatgat acggatcgga atggagttaa tcgcgcgtgg
     7021 cagtgatttc tccggatctt tggtttccgc agctgttgta cctaccagct caatccccac
     7081 gaaagcgaaa accgctatct ggaatccggc aaagaagcca cttaaacctt tcgggaacca
     7141 gccgccgtca ttccacaaat gcgcgaatga cgcttccaca ccggtcggtg actgaaagtg
     7201 catcgccacc atgaccaggc cgacgacaat cagcgacacg atggcgacga ttttgatcat
     7261 cgcaaaccag aactccatct caccgaacat tttcacggtg gcaaggttga gcgtcagcag
     7321 cagcactatc accgccagcg aggcgaccca gtcggagaga tcggggaacc agaactgagc
     7381 ataagccgtg atcgccacca cgtctgccat accggttaca acccagcaga accagtaagt
     7441 ccagccggtg aaatatcccg cccacggccc cagtaaatcg gaagcgaagt cactaaaaga
     7501 tttgtattcc agattcgaaa gcagcaattc ccccattgcc cgcatcacga aaaagagcat
     7561 aaaaccaatg atcatataaa cgaaaatgat cgacggcccg gcaaggctaa tcgttttgcc
     7621 ggaccccata aacaacccag taccaatggc acctccaatg gcaataagct gaacatgtcg
     7681 gtttgtgaga ttgcgccgta gcgactgttc agccggagcc tgatcatcgg caacgacttt
     7741 tacctgatct accatgtttt ttcttcctgt acctgtctgt gttgttcagg ctctacggcc
     7801 tttagtcagt ctatgatacg acgaatctgt atcgggactc atcgatatta ggtaacaata
     7861 cgcgggatga atactaagat ttagatctaa tgttaatttt atgtttaaag tgagtgtcat
     7921 atcacggtga taacgcgaaa cagctcacaa aaatacacgc ttacggggtc tttgcaacac
     7981 aaaatagcgc cgtttggcaa actgaagggt ttattgctga atgcctgctc ccctctggtt
     8041 cgaggggaac agaatgcgct gcttagagga tttccagcag ttcgacttca aacaccaggg
     8101 tgctgaacgg agggatggat gcacctgcgc cgcgctcgcc atatgccagt tcctgcggga
     8161 tagtcagttc ccatttagaa cctaccggca tcagagtcag tgcttcaatc cagccaggga
     8221 tcacgccatt aaccgggaat tcagcgggtt caccacgagc aacggagctg tcaaacacgg
     8281 tgccgtcgat cagtttaccg gtgtaatgaa cacgaacgcg gtcggtacgt gccggaattg
     8341 cgccttcacc ctggttgatc acgcggaatt gcaggccaga ttcggtgcta ttcacacctt
     8401 cttttttggc gttttcttcc aggtatttca cgccttcagc agccatcgcc tggaaacgct
     8461 gacgacgaac ggcatcggcg cgttcgtgga tttcacgcag cgcgcgatgc accacatcaa
     8521 ccggcacagc cggatgtttg ccttccagcg catcggcaat acctgcaacc agtgcttctg
     8581 gcagcagccc ttccaggcca gattcactca gttgttgccc tacctgcaaa ccaatgccgt
     8641 agcttgcttg cgcttcgatg gtgtcaaaag ttggggtggt catcgtattt cctttcttag
     8701 ggtataaaag tagcgggcag catatcagcc acacttcttt gggtaaaact ttgtcgcagg
     8761 aatgatgaca aatcccgggg aaaaagaaac aatagcaata tcccgtatga atataataaa
     8821 ggcgggttta ccacggacat catagcgtta gcttattcat aggctatgat tgaggaacaa
     8881 gacgtggagc aggaggaaac catgcccggg cgctttgaac taaaaccaac cctggagaaa
     8941 gtctggcacg cgccggacaa ttttcgcttc atggacccgc tgccgccgat gcatcgtcgg
     9001 gggatcatta ttgccgccat tgtattggtg gtcggatttc tgcttccatc tgatgatacg
     9061 cccaacgcgt cggtggtgac gcgtgaagcg cagttagata ttcagtcgca atcacaaccg
     9121 ccgacagaag aacagccgca aactacccaa acgcaaccat tccagccaga tagcggaatt
     9181 gataatcaat ggcgctctta ccgtgtcgaa cccggtaaaa cgatggcgca gctgttccgc
     9241 gaccacggac tgcccgctac cgatgtctat gcaatggcgc aggtagaagg cgcgggtaag
     9301 ccgctgagta atttgcaaaa tgggcagatg gtgaaaatcc gccagaacgc cagcggcgtg
     9361 gtgacgggct taaccattga tacggggaat aatcaacagg tcctgtttac ccgccagccg
     9421 gatggcagtt ttattcgtgc gcggtagatg aagggtcaac caaaagccag ctaaggattg
     9481 tctctttgat gaccttacgt ctggcttcca gtgcctgggg ggaaaccata ctgatatgat
     9541 aaactaggcc aagcgtatgc tggttgatca aataatatcc cccaagcgca gcaatattaa
     9601 tattcacctg caatgggtcg atacctggct taaaaatatt gtatttcttc ccttcatcca
     9661 ataaggattc cattaactgc aagtgagcat ggttaatttc taagagacgc tgtgattttg
     9721 cataatgcac acctttactt tgattttcac tatgcacaat ttttaaaaac caaggattag
     9781 caatataata atcccatgta aaatcaataa ttttctcaat ggctttttgt ggcggaagat
     9841 ctgagaaatc aagggccttt tctttttgtc gaatatcgtt ccaggtatat tccagcactt
     9901 cgataaataa attttcttta ttaccaaagt ggtgataaac cgtctgttta ctacaccctg
     9961 ctgcctttac aatattatct actctggccc cttcataacc atattcggca aattcattaa
    10021 ccgcacatag cagcaatttt tccttcaggc tctgtacgtt gctggtatca atatacataa
    10081 cgcttcccca cgacctcttc gtggtgacta tcataacctg atttgatttc agcttcagaa
    10141 aaaagggcaa aaacttgtac tttcatcagg ttatcaaatg tcctgtgatc gccatcgcat
    10201 atcaaccaac tagatggttt atctttttat cctccagtcg caagctaatc atcaccaagg
    10261 aaacaaaatt caaaaaagag agctattgta tgaaatccac tcgtcctgtt gccgtgatta
    10321 ccggagccac tcgcggtatt ggaaagggct gtgcgcagga gcttgctcgt ggcgggttta
    10381 acctgctgat taacgatcgt cccgatgccg acagcgtaga aaaactgcat atcacacaac
    10441 aagaatgttt ggcggaaggc gtggaagtga tttgcttccc tgctgatgtc ggtgatctat
    10501 ctctgcatga ggagatgctc gacgcggcgc aaaaccagtg ggggcggctg gactgtctgc
    10561 tcaataacgc ggggatttca gtgaaaaaac gcggtgacct cctcgacctc gaaccagaca
    10621 gcttcgatca aaacattgcc attaacaccc gcgcgccgtt cttccttgct caggcattca
    10681 gtaagcgctt actggcacag ccgaaaccgg aagcggagct gccgcaccgc tcgattattt
    10741 tcgtcagttc catcaacgcc atcatgctgg cgatgaatcg cggcgaatac accattgcca
    10801 aaaccgccgt ttctgccgct gctcgtctgt ttgctgctcg cctttgtaat gaacaaattg
    10861 gcgtttatga agtgcgccct ggtctcatca aaactgatat gactattccg gcaacggctt
    10921 attacgacga actgattgcc aaaggtctgg tgccgtgggg acgttggggt tatccggctg
    10981 atattgcttc caccgtccgc gcgatggcgg aaggtaaact gatttacacc tgtggtcagg
    11041 cggtcgcaat cgacggcggc ttaagtatgc cgcgcttctg aggctgaatc atgagagatc
    11101 tgttacaacg cccggattta ttcagtatca ataccgcgac actgggctat aaaacgccgc
    11161 tacccgccat tattgatgcc tgcgccgcac gcggtattgg cgcaattgcc ccctggcgca
    11221 gggagttgca gggtgaagat ctccagcaaa tcacccgcca gcttgccgcc agtaacatga
    11281 gcgtttccgg tttgtgccgt agcacttact acaccgcgcc gacactggcg gaacgcaaac
    11341 tggcgattga tgataaccgc cgggcgctgg acgacgcagc ggtgcttaac gccgcctgtt
    11401 atatgcaggt ggttggcggt ctgccaacgg gcactaaaga tctatatgaa gcgcgagaac
    11461 aagtgaaaca agggattcgc cagttgctgc cacattcgaa agatgtcggc gtgccaattg
    11521 cgctggagcc actgcacccg atgacagctg ccgatcgctc ttgcctgtgc acgttacgtc
    11581 aggcgctgga ctggtgtgat gaactcgatc ccgatggtga gtttggcctc ggcgtggcgg
    11641 tggatgttta tcacgtctgg tgggatccgg atctcgccag tcagatcctg cgtgccggaa
    11701 aacgcatcct cgcgtttcat gtttccgact ggttaatgcc gaccactgat ctggtcaatg
    11761 accgtggtat gccgggagat ggcgtcatca atattccgtc aattcgtcgg ctggttgaaa
    11821 acgccggatt taatggtgct attgaactgg aaatattctc gccttactgg tggcagaaag
    11881 atattaacag c
//
LOCUS       DAZOYF010000122        11724 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZOYF010000122 DAZOYF010000000
VERSION     DAZOYF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.123"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..851
                     /locus_tag="ACHUOF_003061"
     CDS             <1..851
                     /locus_tag="ACHUOF_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR0612130.1"
                     /translation="RHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGFN
                     ILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEI
                     VARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEI
                     YESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNP
                     LNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGT
                     IYPIEK"
     gene            873..2282
                     /locus_tag="ACHUOF_003062"
     CDS             873..2282
                     /locus_tag="ACHUOF_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0612131.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="ACHUOF_003063"
     CDS             2260..3039
                     /locus_tag="ACHUOF_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR0612132.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="ACHUOF_003064"
     CDS             3036..3896
                     /locus_tag="ACHUOF_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR0612133.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="ACHUOF_003065"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="ACHUOF_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="ACHUOF_003066"
     CDS             complement(4636..4896)
                     /locus_tag="ACHUOF_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR0612134.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="ACHUOF_003067"
     CDS             complement(4887..6158)
                     /locus_tag="ACHUOF_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0612135.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHUOF_003068"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHUOF_003068"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR0612136.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHUOF_003069"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHUOF_003069"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR0612137.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="ACHUOF_003070"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="ACHUOF_003070"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR0612138.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="ACHUOF_003071"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="ACHUOF_003071"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR0612139.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="ACHUOF_003072"
     CDS             11500..11652
                     /locus_tag="ACHUOF_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0612140.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
BASE COUNT         2814 a         2989 c         3235 g         2686 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggagtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggcggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggcag ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc tgccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacattcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggtgaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc gaagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cctggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt
    11701 ctcctgcacc ctttttttaa attt
//
LOCUS       DAZOYF010000123        11703 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZOYF010000123 DAZOYF010000000
VERSION     DAZOYF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.124"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            38..778
                     /locus_tag="ACHUOF_003073"
     CDS             38..778
                     /locus_tag="ACHUOF_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR0612141.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="ACHUOF_003074"
     CDS             837..1223
                     /locus_tag="ACHUOF_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR0612142.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="ACHUOF_003075"
     CDS             1232..1561
                     /locus_tag="ACHUOF_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR0612143.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="ACHUOF_003076"
     CDS             1533..4574
                     /locus_tag="ACHUOF_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0612144.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="ACHUOF_003077"
     CDS             4574..4903
                     /locus_tag="ACHUOF_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0612145.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..5601
                     /locus_tag="ACHUOF_003078"
     CDS             4903..5601
                     /locus_tag="ACHUOF_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0612146.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5606..6349
                     /locus_tag="ACHUOF_003079"
     CDS             5606..6349
                     /locus_tag="ACHUOF_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0612147.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6313..6888
                     /locus_tag="ACHUOF_003080"
     CDS             6313..6888
                     /locus_tag="ACHUOF_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0612148.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSDGWYQVRIAG
                     RDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIM
                     ASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6949..10428
                     /locus_tag="ACHUOF_003081"
     CDS             6949..10428
                     /locus_tag="ACHUOF_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0612149.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10496..11095
                     /locus_tag="ACHUOF_003082"
     CDS             10496..11095
                     /locus_tag="ACHUOF_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR0612150.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11159..>11703
                     /locus_tag="ACHUOF_003083"
     CDS             11159..>11703
                     /locus_tag="ACHUOF_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0612151.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQ"
BASE COUNT         2750 a         2769 c         3767 g         2417 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     5161 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5221 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5281 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5341 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5401 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5461 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5521 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5581 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5641 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5701 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5761 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5821 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5881 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5941 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     6001 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     6061 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     6121 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6181 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6241 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6301 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6361 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6421 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaattgtc tgcccgtctt
     6481 aatgagccgc tggcaaatgg tgcagtgatc cacatagtac cgcgtctggt gggagctaaa
     6541 agtggcggtg tgtttcaggc ggtgctgggg gcagctgtta tggcggttgc tatatggatg
     6601 ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag tatgacgctt
     6661 ggcggtgttg cacagatgct ggcaccgaaa gccagaactc cccgtacaca gacaacggat
     6721 aacggcaaac agaacacctg tttctcctca ctggataaca tggttgccca gggcaatgtt
     6781 ctgcctgttc tgtacggtga aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc
     6841 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
     6901 catgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
     6961 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg
     7021 agtgtgattg atgccatcag cgaagggccg gttgacggtc cggtggatgg attaaaaagc
     7081 gtgctgctga acggtacgcc ggtcctggat agcgagggga agacaaactt ttccggtgtt
     7141 acggtggtgt tccgcgccgg cgagcaggag cagacaccgc cggaggggtt tgaatcttcc
     7201 ggctcagaga ctgtgctggg tacggaagtg aaatacgaca cgccgatcac ccggaccatc
     7261 acgtcggcaa acattgatcg tctgcgcctg accttcggtg tgcaggcact ggtggaaacc
     7321 acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat acagcgtaac
     7381 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacttc gcagtatctg
     7441 gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta atatccggat gcgcaggatg
     7501 acaccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa
     7561 atcatcgatg tgaaacagtg ctacccgaac acggcgctgg tcggcgtgca ggtggattca
     7621 gagcagttcg gtaaccagca ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag
     7681 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgctt
     7741 aagccagcat acagcaacaa catggcctgg tgtctgtggg acatgctcac tcatccgcgc
     7801 tacggcatgg ggaaacgtct tggtgcggca gatgtggaca aatgggcgct gtatgtcatc
     7861 ggccagtact gcgaccagtc agtgccggac ggatttggcg gcacggagcc gcgcatcacc
     7921 tgtaacgctt acctgaccac acagcgtaag gcgtgggatg ttctcagtga tttctgctcg
     7981 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgaccg
     8041 tcggataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
     8101 ttccgctaca gcttcagcgc cctgaaggac cgccataatg tcgttgaggt gaactggatt
     8161 gatccggaca acggtcatga gacggcgaca gagcttgtgg aggacacgca ggccattgtc
     8221 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg gggacaggcg
     8281 caccgcgccg ggctgtggct gattaaaacg gaactgctgg aaacgcagac cgtggacttc
     8341 agcgtcggcg cagaagggct tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat
     8401 gattatgccg gtatcagcac cggtggtcgc gtactggcgg tgaacagcca gacccggacg
     8461 ctgacgctcg accgtgaaat cacgctgccg tcctccggta ccacgctgat aagcctggtt
     8521 gacggaaatg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg
     8581 aaagtgagcc gggttcctga cggcgttgca gaatacagcg tgtgggggct gaagctgccg
     8641 acgctgcgcc agcgcctgtt ccgctgcgtg agtatccgtg agaacgacga cggcacgtat
     8701 gccatcaccg ccgtgcagca tgtgccggaa aaagaggcca tcgtggataa cggggcacac
     8761 tttgacggtg accggcgcgg cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg
     8821 accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg
     8881 aaggtggtga agggtgtgag cttcctgctt cgcctgaccg tggcagcgga cgacggcagt
     8941 gagcggctgg tcagcacagc caggacgacg gaaaccacat accgcttcag gcagctggcg
     9001 ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca gggcgatccg
     9061 gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga actgataccg
     9121 gggtattttc agataacggt ggtcccgaaa ctggctgtat atgacccgac ggtgcagttt
     9181 gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac cagcgcccgc
     9241 tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc gggccatgat
     9301 tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc
     9361 ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt gatcaataaa
     9421 acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc gccggacctg
     9481 actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa
     9541 aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt tgatacaaat
     9601 aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat
     9661 attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag tcaggtgctg
     9721 ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa gccgatgttt
     9781 gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct gacggctccg
     9841 accattacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
     9901 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
     9961 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
    10021 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
    10081 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca aattgttatt
    10141 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
    10201 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
    10261 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
    10321 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
    10381 ctggttgttg tgatgaagaa atccacagca ggtatcagta tcagctgaat tttataaccc
    10441 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
    10501 taaactttat gctgccattt tgtccgcagc catctgtctg gctgtatccg gtgcgcctgc
    10561 atgggcgtct gaacatcagt ccacgctgag cgcggggtat cttcatgcct cgacgaacgc
    10621 tcccggcagt gatgatctga acgggattaa cgtgaaatac cgttatgagt ttacggacac
    10681 gctggggctg gtgacgtcat tcagctatgc caacgctgaa gatgagcaaa aaacgcatta
    10741 cagtgatacc cgctggcatg aggattccgt gcgtaaccgc tggttcagcg tgatggcggg
    10801 gctgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggcg tggcttacag
    10861 ccgtgtgtcg accttctccg gggattatct ccgcgtaact gacaacaagg ggaaaacgca
    10921 cgatgtgctg accgggagtg atgacaaccg ccacagcaac acgtctctgg cgtggggggc
    10981 tggcgtgcag tttaacccga ccgaatccgt ggccattgac cttgcttatg aaggttccgg
    11041 cagtggcgac tggcgcactg acggtttcat cgtgggtgtc ggttataagt tctgattagc
    11101 caggtaacac agtgttatga cagcccgctg gttcaggcgg gctttttgtg gggtgaatat
    11161 ggcagtaaag atttcaggtg tactgaaaga tggggcgggt aaacctgtcg taaattgtgc
    11221 gattgaactg cgggccagaa gaaccagtcc gaccgttgtg gcacacgttg ttgccacttg
    11281 cgtgacggac aataacggtg cttatgtgat tgaggctgag ccggggtatt acgaggttgc
    11341 gcttcactgt aacggctggc agccaacccg tgtcggggat attgatgtgg caccgactga
    11401 tgcaccgggg acactgaacg cgtttctgaa tgcaccaaag gatggtgatt tacgtccgga
    11461 ggtgatgaag cgctttgagg aaatggtggc gcaggcgcag cagagtgccg gggctgcagc
    11521 cggaaacgca cagcagacgg cgcaggatgt ggcggcagcc gcaacggccc gtgatgatgc
    11581 acaacgtttt gcggagaaag cccgacagga tgcaaccgtc acagctgagg acagaaaggc
    11641 cactgcggaa gatgtgacaa gcacaggagc aaatgcagcc gcagccggac agagcgcaca
    11701 gga
//
LOCUS       DAZOYF010000124        11666 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZOYF010000124 DAZOYF010000000
VERSION     DAZOYF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.125"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(91..912)
                     /gene="bacA"
                     /locus_tag="ACHUOF_003084"
     CDS             complement(91..912)
                     /gene="bacA"
                     /locus_tag="ACHUOF_003084"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0612152.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(1003..1371)
                     /gene="folB"
                     /locus_tag="ACHUOF_003085"
     CDS             complement(1003..1371)
                     /gene="folB"
                     /locus_tag="ACHUOF_003085"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR0612153.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            1476..2093
                     /gene="plsY"
                     /locus_tag="ACHUOF_003086"
     CDS             1476..2093
                     /gene="plsY"
                     /locus_tag="ACHUOF_003086"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR0612154.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(2036..3037)
                     /locus_tag="ACHUOF_003087"
     CDS             complement(2036..3037)
                     /locus_tag="ACHUOF_003087"
                     /inference="COORDINATES: protein motif:HMM:NF007315.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0612155.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     ELLYRSMKFVSARNFWRHGASNGWASPMKAIRSCRSTRNHSGSFSRFLLNFVQIFVS"
     gene            3244..4087
                     /gene="ttdA"
                     /locus_tag="ACHUOF_003088"
                     /pseudo
     CDS             3244..4087
                     /gene="ttdA"
                     /locus_tag="ACHUOF_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            4084..4689
                     /gene="ttdB"
                     /locus_tag="ACHUOF_003089"
     CDS             4084..4689
                     /gene="ttdB"
                     /locus_tag="ACHUOF_003089"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR0612156.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            4737..6200
                     /gene="ttdT"
                     /locus_tag="ACHUOF_003090"
     CDS             4737..6200
                     /gene="ttdT"
                     /locus_tag="ACHUOF_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR0612157.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(6243..7256)
                     /gene="tsaD"
                     /locus_tag="ACHUOF_003091"
     CDS             complement(6243..7256)
                     /gene="tsaD"
                     /locus_tag="ACHUOF_003091"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR0612158.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            7494..7709
                     /gene="rpsU"
                     /locus_tag="ACHUOF_003092"
     CDS             7494..7709
                     /gene="rpsU"
                     /locus_tag="ACHUOF_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR0612159.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            7820..9565
                     /gene="dnaG"
                     /locus_tag="ACHUOF_003093"
     CDS             7820..9565
                     /gene="dnaG"
                     /locus_tag="ACHUOF_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR0612160.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            9760..11601
                     /gene="rpoD"
                     /locus_tag="ACHUOF_003094"
     CDS             9760..11601
                     /gene="rpoD"
                     /locus_tag="ACHUOF_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR0612161.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2863 a         3170 c         2981 g         2652 t
ORIGIN      
        1 ccagcaccgg tgcgaacaat cacccccacc ccggccctct ccctacagag cgagggagaa
       61 aacccggcct gctcccgaac cttttctcaa tcaaaagaac acgacataca ccgcagccgc
      121 cacaataaag cgataaatgg cgaacgggat aaacgaaatg cgcttaatca attgcaggaa
      181 ggttttaatc gctatcagcg ccaccacaaa agcggtgata aacccaacgg caaacatcgg
      241 gatatcgccg gttgtcagga agccccagct tttgtagaga tcgagcgccg ttgcgcccat
      301 catcatcggt accgccagca ggaacgagaa ctcggaagca gcgtaacggc tcacgcccat
      361 cagcatccca cctgaaatgg tcgccccgga acgggagaaa cccggccaca gcgccagaca
      421 ctggaaacag ccaatcataa acgcctgacg ataggtcata tcatcaagac ccggcgcacg
      481 cggctctttc ggcttcaggc attcggcggc aatcagcaac aaaccgccaa cgaccagcgc
      541 atacatcaca tttatcgggt taaacaatga cttaatcgtg tcgtggaata atagccccag
      601 taccaccgcc ggaatcatcc ccagcaaaat gtggatcagc gttaaacgac ctttgctttc
      661 accttcgtgc tgcaacgggc ggccaaagtg gatgccaatc aggccaaaca gacgccgcca
      721 gaacatcact actaccgcca gaattgatcc taactggatc acaacttcaa aggttttcgc
      781 cgtgtcgccc tcaaacccca acaagtgacc gacaataatc atatggcccg tgctggatac
      841 cggcagaaat tctgtcaatc cttcgaccac acccaatatt gccgctatca gcagcgagtg
      901 catatcgctc atcaataaac ccctaaatta ttaaaatgta ccgcttgtcc gaactactgc
      961 agtatgacca ggttataacc gtttggttta acagctgtaa aattaattat tttctttcag
     1021 attattgcca cgctcaatga ttacgccaac attcgccgcc cgcgccactg cgcctggctt
     1081 gctgagtttg atacgcaccc acggcgagtt gaagcgtgct aacagcagct ccgccacctc
     1141 ttcagccacg cgttccacca gcgcaaaacg cgccccctcg acgtggctga ccaccgtttc
     1201 tgcaatgtca gcgtaactga ggcaatccgc cacatcatca cttttcgccg ctttacggtt
     1261 atcccatgcc atttcgatat cgaacactaa cttctgttcg atggtctgtt cccagtcgta
     1321 aacaccaata gtggtgatta ccgaaagttg ctctataaat acaatatcca tcacgtcctg
     1381 cctgcttttt ggctaaccgg ataccacttc cggcgaaatg tgcgtattat ccacagattc
     1441 atcgttgaac acgaattttc aaaacggaac agcttatgag tgcaatcgcg cctggaatga
     1501 tcctcatcgc gtacctctgc ggctccattt ccagtgccat tctggtttgc cgcttgtgtg
     1561 ggctgcccga tccgcgaacc agcggctccg gcaatccagg cgcaaccaat gtgttacgta
     1621 tcggtggcaa gggagcagcc gtagcagtac tgattttcga cgttctgaaa ggaatgttgc
     1681 ctgtctgggg cgcgtatgaa ttaggtgtca gccccttctg gctaggctta attgccatcg
     1741 ccgcctgtct tggacacatc tggcccgttt tcttcggttt taaaggagga aaaggcgttg
     1801 ctaccgcttt tggagcaatc gcaccaatta gctgggatct caccggagta atggcgggaa
     1861 cctggttact gaccgtgcta ttgagcggat actcgtcgct gggagcgatt gtcagtgcac
     1921 tgattgctcc gttttatgtc tggtggttta agccacaatt caccttcccg gtttcgatgc
     1981 tctcttgcct gatcctgctg cgtcatcatg acaacatcca gcgtctgtgg cgtcgtcagg
     2041 agacaaaaat ctggacgaaa ttcaaaagaa agcgcgaaaa ggatcccgag tgatttctgg
     2101 tggatctaca tgacctgata gccttcatcg ggcttgccca gccgttgctg gcaccatgcc
     2161 gccagaaatt ccgcgcagac acgaatttca tgctgcgata gagcagctcc cggtaaacag
     2221 cccagatatt ggcgctttgt gcatactccg gcaatacttg caccagtttg ccactctcca
     2281 gaaacggcaa cacatcccac tcggaacgca gcataatccc tttgccctcc agcgcccatt
     2341 gcaacactat ctcgccgcta ttggaggaaa gatgcccgct aaccttcacc gattttttct
     2401 cctgaccgtt ccccaactcc catattccgt gggtcatatc gcgctctttg gtcaccagac
     2461 agtcatgacg acttaattcc tgtaatgatt gtggctgcgg atatttttgc agatactcag
     2521 gtgctgcgca taatattctt ttatttttcg ttaacagatg cgcaatataa taatcaggaa
     2581 tttcgtcatt aatacgaata tccagatcaa tattatcctg cactaaatca atttgccgat
     2641 cgaacaattc aaaatgcacc tgtaactcag gataattgcg cataagttcg gtaatggctg
     2701 gcgcaatatg gctgcgccca aaaccaaaac tgcaaccaat acgaatcatc ccctccgggc
     2761 gcgttttgat ttgcgtgacg tcatcgacca gtcgctgata ttgggtgagg atttccagcg
     2821 catgttcgta acaacgttgg ccactttcag tcagcgccac gccgcgagcg gagcggttca
     2881 gcagcgttgt tgccagcgta ttttcaagga tttggatccg cttggtgaca aatgcaggcg
     2941 tttgcccaag cgtcgccgct gcagcactaa aactcccgct gtggacgatt tctaccagta
     3001 cctgcaagtc tttggctaaa ggccagctat tcagcatcgt taattatccg cagttgtgat
     3061 aagcgcagtg tatttcgcaa aacattgcca ttccctgctc tggcgcaata ttcattctta
     3121 ttttcgcgtt gattcacgaa ctgttaatta catattaacg gtggcaaaaa atataaaacc
     3181 acatttttag tgttagtttg tggcggtgaa tttttccaga caaatacaaa aactggagtt
     3241 gccatgatga gcgaaagtaa taagcaacag gcagtgaata agttgacaga gattgtcgct
     3301 aactttaccg ccatgatttc tacccgaatg cctgatgacg tggtggataa actaaaacag
     3361 ctaaaggatg ccgaaacgtc gtcgatgggg aaaattatct accatacgat gttcgacaac
     3421 atgcaaaaag cgattgacct gaatcgtcct gcctgtcagg acaccgggga gattatgttc
     3481 ttcgttaaag tcggttcccg cttcccactg cttggcgagc tgcaaagcat actcaaacaa
     3541 gccgtggaag aggcaaccgt caaagcgcca ctacgtcaca atgcggtaga aatttttgac
     3601 gaagtaaaca ccggcaaaaa taccggtagc ggcgtaccgt gggtcacctg ggacatcatc
     3661 cccgacaatg acgatgcgga aatcgaagtt tacatggcag gcggcggctg cacgctacct
     3721 ggccgctcga aagtgttaat gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc
     3781 gaaaatatct ccaccctcgc cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc
     3841 gccacctcgg tggaaaccgc cgccgtactc tcgcgtaaag ccattttgcg cccgattggc
     3901 agccgccatc ccaatccaaa agcggcagaa ctggagctac gcctggaaga aggactcaac
     3961 cgtctgggga ttggtccaca agggctgacc ggcaacagtt cagtgatggg cgcatcgtcg
     4021 cggcacgctg ctggttcatg ccgatctcac ctttgaaaat ctgtctcaca cccggagcgc
     4081 gttatgaaaa agatcctgac aaccccgatc aaagctgaag atctgcaaga tattcgcgtc
     4141 ggcgatgtga tctacctgac cggtacgctg gtgacctgcc gcgacgtttg tcaccgccgt
     4201 ttgatcgaac tgaaacgtcc gatcccttac gatctcaacg gcaaagcgat tttccacgct
     4261 ggccccatcg tgcgcaaaaa cggcgacaaa tgggagatgg tctccgtcgg cccgacaacc
     4321 agtatgcgta tggaaagttt tgaacgtgaa tttattgagc agaccggcgt aaaactggtg
     4381 gttggcaaag gtggtatggg gccgctgacc gaagaaggct gccagaaatt caaggcgtta
     4441 catgtgattt tcccagcagg ctgcgcggtg ctggcggcaa cccaggtaga agagattgaa
     4501 gaagtgcact ggacagaact cgggatgccg gagtcactgt gggtctgccg ggtcaaagag
     4561 ttcggcccgc tgattgtctc tattgatacc cacggcaaca acctgatagc cgaaaacaaa
     4621 aagctgttcg ccgaacgccg cgatcccatc gtggaagaga tctgcgagca cgtccattac
     4681 atcaaataac cctcccggag aggctcaccc ctctcctttt tcgcaggcat aacacgatga
     4741 aaccttccac tgaatggtgg cgatatcttg cgccgctggc ggtcatcgcc attattgctc
     4801 tacttccggt tcccgcaggg ctggagaacc atacctggct ctactttgcc gtttttactg
     4861 gcgtgatcgt tggactgatc ctcgaacccg tgccgggtgc cgtggtggcg atggtgggta
     4921 tctccatcat cgccatactc tctccctggc tgctgttcag ccccgaacag ctcgcccagc
     4981 caggctttaa gttcaccgcc aaatccctct cctgggcagt ttctggtttt tctaattcgg
     5041 ttatctggct cattttcgcc gcctttatgt ttggcacagg ctatgaaaaa accgggcttg
     5101 gacgccgcat tgcgctgatt ctggtgaaaa agatggggca tcgcacgctg tttctcggct
     5161 atgcggtgat gttctccgag ctgatcctgg cacctgtaac accgtccaac tcggcgcgtg
     5221 gtgcgggtat tatctatccc atcatccgta acctgccacc gctctatcaa tcacaaccaa
     5281 acgacagcag ttcgcgcagc attggctcgt acatcatgtg gatggggatt gttgccgact
     5341 gcgtgaccag cgccattttc ctgacggcga tggcacctaa cttgctgtta attggactga
     5401 tgaaaagcgc atctcacgcc acgctgagtt ggggcgactg gttcctcggg atgttgccgc
     5461 tcagcatttt actggttctg ctggttccct ggctggctta cgtgctgtac ccgccggtac
     5521 tgaagtctgg tgatcaggtg ccgcgctggg cagagacgga actgcaggca atgggcccgc
     5581 tctgttcgcg tgaaaaacgg atgctggggc tgatggtagg cgcgctggtg ctgtggattt
     5641 tcggcggtga ttatatcgat gccgcgatgg tcggttacag cgtggtggcg ctgatgctgc
     5701 ttctgcgcat tatcagttgg gacgacattg tcagtaataa agcggcatgg aacgttttct
     5761 tctggctggc ctcgcttatc accctcgcga ccggactcaa caataccggt tttattagct
     5821 ggtttggcaa actgttagca ggcagcttaa gtggttattc gccgacgatg gtgatggtga
     5881 cgttgattgt ggtgttttat ctactgcgct actttttcgc cagcgccacg gcgtatacct
     5941 gcgccctcgc accgatgatg atcgccgccg cgctggcgat gccggaaatc ccgctgccgg
     6001 tgttctgcct gatggttggc gcggcaattg gtctggggag cattcttaca ccatacgcca
     6061 ccggacccag tccgatttac tacggtagtg gttatctgcc aacggcggat tactggcgac
     6121 tgggggcgat ttttgggctg atattcctcg tgttgctggt gattaccggc ttactgtgga
     6181 tgcccgtggt gttgctttaa gtgaggacgg tgccggaaat tgctctccgg cacccaatag
     6241 ttttacgcag ccggtaactc cgccagcggc cagcgcggac gcacgctaac gccgagatcc
     6301 gccgtcgcgc ctgctttaaa ccgcaccatt ccggcatagg cgatcatcgc gccgttatca
     6361 gtacaaaatt ccggacgcgc gtagaacact tcgccgcggc gttttttcat catttcagcc
     6421 agcttcgccc gtaacgtgcg gttagcactc acgccgcccg ccatgaccag tcgcttaaag
     6481 cccgtctgat ccaacgctcg cttacactta atcatcaacg tatcgaccac cgcatcttca
     6541 aaggcgcggg cgatatcagc acgcgtctgg tcgtcggtgc cgttgtcacg aatcgtgttc
     6601 gccgcaaagg ttttcagacc agaaaagctg aaatccagcc ccggacggtc ggtcatcgga
     6661 cgcgggaaaa caaagcgccc ggcagtaccc tgagccgcca ttttcgacag taacggtccg
     6721 ccaggataat ccagccccag cagcttcgcg gttttatcaa acgcttcacc ggcggcatca
     6781 tcgatagact cgccgagcag ctcgtactga ccaatgccag tcacgctgat taactgcgta
     6841 tgaccgccgg acaccagcag cgcgacaaac ggaaattccg gcgggttatc ttccagcatc
     6901 ggcgctaaca gatgcccttc catatggtgt accgggattg ccggaacgtt ccaggcaaac
     6961 gccagagaac gccccacggt tgcgccaacc agcagtgcgc cgactaatcc agggcctgcg
     7021 gtataggcca cagcatcaat gtcttttgcc gttaaaccag actcctttag cgccgcctgg
     7081 atcaacggta cggttttacg cacatgatcg cgggaggcca gttcaggcac gacgccgccg
     7141 tagtcagcgt gcaatttcac ctgactatac aattggttgg ctaacaaacc tttttcatcg
     7201 tcgtaaatgg cgatgccggt ttcatcgcag gaagtttcaa tacccagtac acgcatgact
     7261 tgttttacct cgctttatta ccgcgcagtg taggaccaat gcgggttgat gtaaaacttt
     7321 gttcgcccct ggagaaagcc tcgtgtatac tcctcaccct tataaaagtc cctttcaaaa
     7381 aaggccgcgg tgctttacaa agcagcagca attgcagtaa aattccgcac cattttgaaa
     7441 taagctggcg ttgatgccag cggcaaaccg aattaatcaa aggtgagagg cacatgccgg
     7501 taattaaagt acgtgaaaac gagccgttcg acgtagctct gcgtcgcttc aagcgttcct
     7561 gcgaaaaagc aggtgttctg gcggaagttc gtcgtcgtga gttctatgaa aaaccgacta
     7621 ccgaacgtaa gcgcgctaaa gcttctgcag tgaaacgtca cgcgaagaaa ctggctcgcg
     7681 aaaacgcacg ccgcactcgt ctgtactaat tccccgagag cgttgctctc cgatcagacc
     7741 gagttgtagt tgtaaggccg tgcttccgaa aggaatgcgc ggcttatttt cgtttatgaa
     7801 ttgctaaaaa tcggggccta tggctggacg aatcccacgc gtattcatta atgatctgct
     7861 ggcacgcact gacatcgtcg atctgatcga tgcccgtgtg aagctgaaaa agcagggcaa
     7921 gaatttccac gcgtgttgtc cattccacaa cgagaaaacc ccgtcattca ccgttaacgg
     7981 tgagaaacag ttttaccact gctttggatg tggcgcgcac ggcaacgcga tcgacttcct
     8041 gatgaactac gacaagctcg agttcgtcga aacggtcgaa gagctggcag caatgcacaa
     8101 tcttgaagtg ccatttgaag caggcagcgg ccccagccag atcgagcgcc atcagaggca
     8161 aacgctttat cagttgatgg acggtctgaa tacgttttac caacaatctt tacaacaacc
     8221 tgttgccacg tctgcgcgcc agtatctgga aaaacgcgga ttaagccacg aggttatcgc
     8281 tcgctttgcg attggttttg cgccccccgg ctgggacaac gtcctgaagc ggtttggcgg
     8341 caatccagaa aatcgccagt cattgattga tgcggggatg ttggtcacta acgatcaggg
     8401 acgcagttac gatcgtttcc gcgaacgggt gatgttcccc attcgcgata aacgcggtcg
     8461 ggtgattggt tttggcgggc gcgtgctggg caacgatacc cccaaatacc tgaactcgcc
     8521 ggaaacagac attttccata aaggccgcca gctttacggt ctttatgaag cgcagcagga
     8581 taacgctgaa cccaatcgtc tgcttgtggt cgaaggctat atggacgtgg tggcgctggc
     8641 gcaatacggc attaattacg ccgttgcgtc gttaggtacg tcaaccaccg ccgatcacat
     8701 acaactgttg ttccgcgcga ccaacaatgt catttgctgt tatgacggcg accgtgcagg
     8761 ccgcgatgcc gcatggcgag cgctggaaac ggcgctgcct tacatgacag acggccgtca
     8821 gctacgcttt atgtttttgc cggatggcga agaccctgac acgctggtac gaaaagaagg
     8881 taaagaagcg tttgaagcgc ggatggagca ggcgatgcca ctctccgcat ttctgtttaa
     8941 cagtctgatg ccgcaagttg atctgagtac ccctgacggg cgcgcacgtt tgagtacgct
     9001 ggcactgcca ttgatatcgc aagtgccggg cgaaacgctg cgaatatatc ttcgtcagga
     9061 attaggcaac aaattaggca tacttgatga cagccagctt gaacgattaa tgccaaaagc
     9121 ggcagagagc ggcgtttctc gccctgttcc gcagctaaaa cgcacgacca tgcgtatact
     9181 tatagggttg ctggtgcaaa atccagaatt agcgacgttg gtcccgccgc ttgagaatct
     9241 ggatgaaaat aagctccctg gacttggctt attcagagaa ctggtcaaca cttgtctctc
     9301 ccagccaggt ctgaccaccg ggcaactttt agagcactat cgtggtacaa ataatgctgc
     9361 cacccttgaa aaactgtcga tgtgggacga tatagcagat aagaatattg ctgagcaaac
     9421 cttcaccgac tcactcaacc atatgtttga ttcgctgctt gaactgcgcc aggaagagtt
     9481 aatcgctcgt gagcgcacgc atggtttaag caacgaagaa cgcctggagc tctggacatt
     9541 aaaccaggag ctggcgaaaa agtgatttaa cggcttaagt gccgaagagc gatcgggaag
     9601 cccccgacag ccgcactgag aggcagcggc aaatatataa gtacgccctc gtaattatcg
     9661 ttggcggtaa acaaccgttg gatttcagcg ttaacggctg aaggacatcg ggtcaatcgc
     9721 ccaacaccaa cctcatgaaa taagtgtgga taccgtctta tggagcaaaa cccgcagtca
     9781 cagctgaaac ttcttgtcac ccgtggtaag gagcaaggct atctgaccta tgccgaggtc
     9841 aatgaccatc tgccggaaga tatcgtcgat tcagatcaga tcgaagacat catccaaatg
     9901 atcaacgaca tgggcattca ggtgatggaa gaagcaccgg atgccgatga tctgatgctg
     9961 gctgaaaaca ccgcggacga agatgctgcc gaagccgccg cgcaggtgct ttccagcgtg
    10021 gaatctgaaa tcgggcgcac gactgacccg gtacgcatgt acatgcgtga aatgggcacc
    10081 gttgaactgt tgacccgcga aggcgaaatt gacatcgcta agcgtattga agacgggatc
    10141 aaccaggttc aatgctccgt tgctgaatat ccggaagcga tcacctatct gctggaacag
    10201 tacgatcgtg ttgaagcaga agaagcgcgt ctgtccgatc tgatcaccgg ctttgttgac
    10261 ccgaacgcag aagaagatct ggcacctacc gccactcacg tcggttctga gctttcccag
    10321 gaagatctgg acgatgacga agatgaagac gaagaagatg gcgatgacga cagcgccgat
    10381 gatgacaaca gcatcgaccc ggaactggct cgcgaaaaat ttgcggaact acgcgctcag
    10441 tacgttgtaa cgcgtgacac catcaaagcg aaaggtcgca gtcacgctgc cgctcaggaa
    10501 gagatcctga aactgtctga agtattcaaa cagttccgcc tggtgccgaa gcagtttgac
    10561 tacctggtca acagcatgcg cgtcatgatg gaccgcgttc gtacgcaaga acgtctgatc
    10621 atgaagctct gcgttgagca gtgcaaaatg ccgaagaaaa acttcattac cctgtttacc
    10681 ggcaacgaaa ccagcgatac ctggttcaac gcggcaattg cgatgaacaa gccgtggtcg
    10741 gaaaaactgc acgatgtctc tgaagaagtg caccgcgccc tgcaaaaact gcagcagatt
    10801 gaagaagaaa ccggcctgac catcgagcag gttaaagata tcaaccgtcg tatgtccatc
    10861 ggtgaagcga aagcccgccg tgcgaagaaa gagatggttg aagcgaactt acgtctggtt
    10921 atttctatcg ccaagaaata caccaaccgt ggcttgcagt tccttgacct gattcaggaa
    10981 ggtaacatcg gcctgatgaa agcggttgat aaattcgaat accgccgtgg ttacaagttc
    11041 tccacctacg caacctggtg gatccgtcag gcgatcaccc gttctatcgc ggatcaggcg
    11101 cgcaccatcc gtattccggt gcatatgatt gagactatca acaaactcaa ccgtatttct
    11161 cgccagatgc tgcaagagat gggccgtgaa ccgacgccgg aagaactggc tgaacgtatg
    11221 ctgatgccgg aagacaagat ccgcaaagtg ctgaagatcg ccaaagagcc aatctccatg
    11281 gaaacgccga tcggtgatga tgaagattcg catctggggg atttcatcga ggataccacc
    11341 ctcgagctgc cgctggattc tgcgaccacc gaaagcctgc gtgcggcaac gcacgacgtg
    11401 ctggctggcc tgaccgcgcg tgaagcaaaa gttctgcgta tgcgtttcgg tatcgatatg
    11461 aacaccgacc acacgctgga agaagtgggt aaacagttcg acgttacccg cgaacgtatc
    11521 cgtcagatcg aagcgaaggc gctgcgcaaa ctgcgtcacc cgagccgttc tgaagtgttg
    11581 cgtagcttcc tggacgatta atcggtaggc cggatcaggc gttacgccgc acccggcact
    11641 agtctctctg cacaaacgcc accttt
//
LOCUS       DAZOYF010000125        11657 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZOYF010000125 DAZOYF010000000
VERSION     DAZOYF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.126"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..794
                     /locus_tag="ACHUOF_003095"
     CDS             <1..794
                     /locus_tag="ACHUOF_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HFR0612162.1"
                     /translation="SETKAAYRCFSNDKVSANKIMTPHKENITERAQQFKRVLVLQDT
                     TELNYSGQKEKQGVGPKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKN
                     TRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFER
                     AAQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQ
                     ARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="ACHUOF_003096"
     CDS             complement(744..1937)
                     /locus_tag="ACHUOF_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0612163.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="ACHUOF_003097"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="ACHUOF_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR0612164.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="ACHUOF_003098"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="ACHUOF_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR0612165.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="ACHUOF_003099"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="ACHUOF_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR0612166.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="ACHUOF_003100"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="ACHUOF_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR0612167.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="ACHUOF_003101"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="ACHUOF_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR0612168.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="ACHUOF_003102"
     CDS             complement(10573..10716)
                     /locus_tag="ACHUOF_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612169.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..>11657
                     /locus_tag="ACHUOF_003103"
     CDS             10955..>11657
                     /locus_tag="ACHUOF_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR0612170.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWD"
BASE COUNT         2956 a         2898 c         2992 g         2811 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tgggact
//
LOCUS       DAZOYF010000126        11453 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZOYF010000126 DAZOYF010000000
VERSION     DAZOYF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.127"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(67..2823)
                     /gene="barA"
                     /locus_tag="ACHUOF_003104"
     CDS             complement(67..2823)
                     /gene="barA"
                     /locus_tag="ACHUOF_003104"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR0612171.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            2880..4181
                     /gene="rlmD"
                     /locus_tag="ACHUOF_003105"
     CDS             2880..4181
                     /gene="rlmD"
                     /locus_tag="ACHUOF_003105"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR0612172.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            4229..6463
                     /gene="relA"
                     /locus_tag="ACHUOF_003106"
     CDS             4229..6463
                     /gene="relA"
                     /locus_tag="ACHUOF_003106"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR0612173.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            6559..7350
                     /gene="mazG"
                     /locus_tag="ACHUOF_003107"
     CDS             6559..7350
                     /gene="mazG"
                     /locus_tag="ACHUOF_003107"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR0612174.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            7578..9215
                     /gene="pyrG"
                     /locus_tag="ACHUOF_003108"
     CDS             7578..9215
                     /gene="pyrG"
                     /locus_tag="ACHUOF_003108"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR0612175.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            9303..10601
                     /gene="eno"
                     /locus_tag="ACHUOF_003109"
     CDS             9303..10601
                     /gene="eno"
                     /locus_tag="ACHUOF_003109"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR0612176.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(10661..11453)
                     /locus_tag="ACHUOF_003110"
                     /pseudo
     CDS             complement(10661..11453)
                     /locus_tag="ACHUOF_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
BASE COUNT         2916 a         2756 c         2997 g         2784 t
ORIGIN      
        1 aggcactttg tcaccaatct gaaaccagcg tcataaaaag ccgattgcta ctcgacaaga
       61 cgtccgttac ccgagaattt tgctggcttc gcgcgcgaca ttatccatct cgtccaacag
      121 ttccagcagc tccggctcca aatcttcttc tttagtaccg ctacgtagct gttgttcgat
      181 cagctggcag agattcttca tacgtggtac gccgctatag ccgcaactgc catgcagttt
      241 atgaatcaaa tcaaccaggc cttccgggtt ttctccagcc agctgttcct caactttgtt
      301 gcgaacttca ggcaggaaat cgagtaacat ttgcagcata tcgcgcgcta aatcggtttt
      361 tcctgctgcc tggcgtagtg ccagttgcca gtcgagggtc gcattcgggt tcaccacaat
      421 ttcgttgact tcgggcgtca cgacgcgaga cgaaataccg ctgccaggct tgtagcgcaa
      481 caacaaatta tgcaatcgct cttcttcaat cggtttcgcc agataatcgc tcatccctgc
      541 gccaagcagc ttctcttttt gcccggccat tgcatgcgcc gttaccgcga taaccggcgt
      601 ttgtcgctga tgcgggagtt ggtggatgag ttcgcaggcc cgaatgccat ccatgtccgg
      661 catttgaata tccattaaga tcaaatcgaa cggcatctgt ttcgcccgtt caactgcctg
      721 atgcccgcta tcgcaaagtt ccacatgttg caccatatct tccagcaatg cgccgataag
      781 tttcaggtta gcggggttgt catcaaccgc catgactgtc attgccagct tactttcatc
      841 ggttacaggc aaaagcgtgt tttgtttgtg atgacaaaat tccgtcaggg caggcaacag
      901 gcgcgtaggt gttaatggtt tcagcagaca cgcgccgata ccatcttgct tgagtttttc
      961 agcattaact tgtgcatggc aaggaagtgc cagcatcagg aaatcggtca tcgataccgc
     1021 tttcgctaat cgctcatgtt gcattgttag cggctcgcgg aaggtcaccg cgatgcctaa
     1081 caacatcatg tcgtaatgcg cgggaggcag cgcggagaac gttgggctat aaaccacttc
     1141 cagcggcgtt tcacttaaaa tatccagcgt gcattgcgct gctgcggagt ttggttcgac
     1201 ataggccagg cgcttacctg cgaggcactg gatggatggc ccttcgataa taatgttcgg
     1261 gttcagatcg agattaatgt ggaaccagaa agttgaaccg cgattcggct ggctatggaa
     1321 cgaaatatcg ccgcccattt cattaaccag tttttgtgta atcaccagcc ccagcccggt
     1381 gccaccatga cggcgggaaa tactggcatc agcctgtcgg aaggcctgga ataagcgcga
     1441 ttgatcgcgt tcaggaatac caatgccggt atcccgaatc tgcacttcaa tctgcacttt
     1501 ggtattactc agcgcacgtt tttctaccag aatatcaatg ttgccattct cggtgaattt
     1561 aattgcattc cccaccaggt tagtgatgat ttgctgtaat cgcagtgggt cgccgatcac
     1621 gttatcaggc acgtcgcttt taatattgag cgtcagttcc aaccctttat cgtgagaaga
     1681 atgtgccagc agagtaacga cttcatccag cgtgctgcgt agtgggaatg gaatactttc
     1741 cagaatcagc ttacctgctt ccagtttcga gaagtcgaga acatcattaa taattgccag
     1801 caaattattt gccgaacgtt caatcgtatt caggtgatcg cgctgcgttg gtgttaattc
     1861 tgttttcagc gtcaggcggg taaagccaat aacaccattc agtggtgtac gcagctcgtg
     1921 tgacatattt gccagaaact cggatttaat acgcgccgct tcctgggcgc gctttttcgc
     1981 cagatccaac tcaacgttct gaatttccat ctgctccagc gtttcacgca gatcggacgt
     2041 cgcctggtcg atattgtgct gcatctcttc gtgataagca gccagcgaca ttgccatcga
     2101 gttgataccg tttttcagca tatccagctc gccgagcata aatccttcca cccggctgtc
     2161 gagttgcccg cgacggatgc ggtcgacggt attcaccatg ttgcgaatcg gaccggttac
     2221 atcgcgcatt aagcgccagc caaaaataag cgcaataccg atacaaaaca gcatcatcac
     2281 gcaggaaata aagatctctt tatattgctg caagcgaacc gatttaagat ccagctcaag
     2341 cgcaatatat cccaacatat tttgactatt tttggcatcg ctactgggcg attcgtcggg
     2401 ggagtaactc tcagaaataa tcggcgtgcg gaggatcata atatcgccgt cacgagtgac
     2461 agtgagctgg cgaggaaacg gcacgttgct gccgagctgc attgatgagg gatcaagatg
     2521 aaaattggag gtgacaaaga gtcggttatt ttcatcataa accgaaatcg cgcgaacaat
     2581 atcggaatgg cgacgatgca gtacgcttat taactgaccg atagattcgc gattttgcag
     2641 gctcatgcca tattcagtag aaactgcaag cggctcaata atgctagcac cggcatcttc
     2701 cagttgacgc tgcaagtcgt tatagcgatg cacgacgaaa aagatactca gcaataaacc
     2761 aataaggacg gtcggtgcca ggatcagaat catcatgcgt gcgcgcaggc tgtagttggt
     2821 catggagttc cgttatggga caattaaggt cacactgtta aattgagaaa gtctcgccaa
     2881 tggcgcaatt ctactctgca aaacgacgca cgacgacgcg tcagatcata accgtttcag
     2941 tcaacgacct cgactctttt ggtcagggcg tggcgcgaca taacggcaaa acgttattta
     3001 tccccggatt attgccgcag gaaaacgcgg aagttgctgt tactgaagat aaaaaacagt
     3061 atgcccgcgc taaagtcgta cgccggttaa gcgatagccc ggaacgcgaa acgccacgct
     3121 gtcctcattt tggcgtatgc ggcggctgtc agcaacaaca cgccagcgtg gatttacagc
     3181 agcgaagcaa aagtgcggca ctcgcccgat taatgaaaca cgatgtctct gaagtgatcg
     3241 ccgatgttcc ctggggctat cgccgccgcg cgcgtttaag tctgaactac ttaccgaaaa
     3301 cacagcaact tcagatgggg tttcgcaaag cgggctccag tgacattgtc gacgtcaaac
     3361 aatgccccat tttagcgccc caacttgaag cattgctgcc caaagtcagg gcatgtctgg
     3421 gcagcttaca agctatgcgc catcttggtc atgttgaact ggtacaggca accagcggca
     3481 cgctgatgat tttgcgccat accgcaccgc taagttcggc agatcgcgaa aaactggaac
     3541 gcttttcgca ttctgaaggc ctggatctgt atctcgcccc cgatagtgag atactcgaaa
     3601 ccgtctctgg tgagatgccc tggtatgact caaacgggtt gcgcttaact tttagcccgc
     3661 gcgattttat tcaggtcaat gcgggtgtga accaaaaaat ggtagcgcgt gcgttggaat
     3721 ggctggatgt gcaacctgaa gatcgcgtac tggatctgtt ctgcggtatg ggcaacttta
     3781 cactgccatt ggcgacacaa gctgccagtg tggtcggtgt agaaggtgtt cctgcgctgg
     3841 tggaaaaagg ccagcagaat gcgcgtctta atggcttaca gaatgtgacg ttttatcacg
     3901 aaaatcttga agaagatgtc acaaagcagc cgtgggcgaa aaacggcttc gataaagtgt
     3961 tgctggaccc ggcgcgagca ggtgccgcag gtgttatgca gcaaattata aaactggaac
     4021 ctattcgtat agtttatgta tcctgtaacc ctgcaacgct ggctcgggat agcgaagcgt
     4081 tattaaaagc aggatatacc attgcgcgac tggcgatgct ggatatgttc ccacacacgg
     4141 gacatctgga atcgatggta cttttctcgc gcgttaaata gttacgattt gccgatttcg
     4201 gcaggtctgg tccctaaagg agaggacgat ggttgcggta agaagtgcac atatcaataa
     4261 ggctggtgaa tttgatccgg aaaaatggat cgcaagtctg ggtattacca gccagaagtc
     4321 gtgtgagtgc ttagccgaaa cctgggcgta ttgtctgcaa cagacgcagg ggcatccgga
     4381 tgccagtctg ttattgtggc gtggtgttga gatggtggag atcctctcga cattaagtat
     4441 ggacattgac acgctgcggg cggcgctgct gttccctctg gctgatgcca acgtagtcag
     4501 cgaagatgtg ctgcgtgaga gcgtcggtaa gtcggttgtt aaccttattc acggcgtgcg
     4561 tgatatggcg gcgatccgcc agctgaaagc gacgcacact gattctgttt cctccgaaca
     4621 ggtcgataac gttcgccgga tgttattggc gatggtcgat gattttcgct gcgtagtcat
     4681 caaactggcg gagcgtattg ctcatctgcg cgaagtaaaa gatgcgccgg aagatgaacg
     4741 tgtactggcg gcaaaagagt gcaccaatat ctacgcgccg ttggcgaacc gtcttgggat
     4801 tgggcaactg aaatgggagc tggaagatta ctgcttccgt tatctccacc cgaccgaata
     4861 caaacgcatc gcaaaactgc tgcatgaacg ccgtctcgac cgcgaacact atatcgaaga
     4921 gtttgtcggc catctgcgcg ctgagatgaa agctgaaggc gttaaagctg aagtgtatgg
     4981 tcgtccgaaa cacatctaca gcatctggcg caaaatgcag aaaaagaacc tcgccttcga
     5041 tgagctgttt gatgtgcgtg cggtacgtat tgtcgccgag cgtttacagg attgttatgc
     5101 cgcactgggg atagtgcaca ctcactatcg ccacctgccg gatgagtttg acgattacgt
     5161 cgctaacccg aaaccaaacg gttatcagtc tattcatacc gtggttctgg ggccgggtgg
     5221 aaaaaccgtt gagatccaaa tccgcaccaa acagatgcat gaagatgcgg agttgggtgt
     5281 tgctgcgcac tggaaatata aagagggcgc ggcagctggc ggcgcacgtt cgggacatga
     5341 agaccggatt gcctggctgc gtaaactgat tgcgtggcag gaagagatgg ctgattccgg
     5401 cgaaatgctc gacgaagtac gtagtcaggt gtttgacgac cgggtgtatg tctttacgcc
     5461 gaaaggtgat gtcgttgatt tgcctgcggg atcaacgccg ctggacttcg cttaccacat
     5521 ccacagtgat gtcggacacc gctgcatcgg ggcaaaaatt ggcgggcgca ttgtgccgtt
     5581 cacctaccag ctgcaaatgg gcgaccagat tgaaattatc acccagaaac agccgaaccc
     5641 cagccgtgac tggttaaacc caaacctcgg ttacgtcaca accagccgtg ggcgttcgaa
     5701 aattcacgcc tggttccgta aacaggaccg tgacaaaaac attctggctg ggcggcaaat
     5761 ccttgacgac gagctggaac atctggggat cagcctgaaa gaagcagaaa aacatctgct
     5821 gccgcgttac aacttcaatg atgtcgacga gttgctggcg gcgattggtg gcggggatat
     5881 ccgtctcaat cagatggtga acttcctgca atcgcaattt aataagccga gtgccgaaga
     5941 gcaggacgcc gccgcgctga aacagcttca gcaaaaaagc tacacaccgc aaaaccgcag
     6001 taaagataac ggtcgtgtgg tggttgaagg tgttggtaac ctgatgcacc acatcgcgcg
     6061 ctgctgccag ccgattcctg gagatgagat tgtcggcttc attacccagg gacgcggtat
     6121 ttcagtacac cgcgccgatt gcgaacaact ggcggaactg cgctcccatg cgccagaacg
     6181 cattgttgac gcggtatggg gtgagagcta ctccgccgga tattcgctgg tggtccgcgt
     6241 ggtggctaat gatcgtagtg ggttgttacg tgatatcacg accattctcg ccaacgagaa
     6301 ggtgaacgtg cttggcgttg ccagccgtag cgacaccaaa cagcaactgg cgaccatcga
     6361 catgaccatt gagatttaca acctgcaagt gctggggcgc gtgctgggta aactcaacca
     6421 ggtgccggat gttatcgacg cgcgtcggtt gcacgggagt tagtcttaca tagctggata
     6481 tggcgtcagc cgtatccagc gatatctttt actcgaaggt attacacctg acagacccgc
     6541 aattttcagg acaattcaat gaatcaaatc gaccgtttgc tcactattat gcagcgcctg
     6601 cgcgatccgg aaaacggctg cccgtgggat aaagagcaga catttgccac cattgcgcct
     6661 tacacccttg aagaaaccta cgaagtgctg gacgccatcg cccgtgaaga ttttgacgat
     6721 cttcgcggtg aactgggcga tctgctattc caggtggtgt tttacgcgca aatggctcag
     6781 gaagaagggc gctttgactt taatgatatt tgcgctgcta ttagcgataa attagagcgt
     6841 cgccatccgc atgtttttgc tgatagttct gccgaaaaca gtagtgaagt gcttgcccgt
     6901 tgggagcaaa tcaaaaccga agagcgcgcg cagaaagcgc agcattcggc gctggacgat
     6961 attcctcgta gtttaccggc tttaatgcgg gcgcaaaaaa tccagaaacg ttgcgccaac
     7021 gttggcttcg actggacgac gcttggcccg gtggttgata aagtctacga agagatcgac
     7081 gaggtgatgt acgaggcgcg gcaggctgtt gtcgaccagg ctaaactgga ggaggaaatg
     7141 ggggacctgc tgtttgccac ggttaatctg gctcgccatt tagggacgaa agcagaaatc
     7201 gcattgcaaa aagtgaacga aaaattcgag cgtcgttttc gcgaagtgga gcgtattgtt
     7261 gccgcgcgtg gactggaaat gacaggtgtt gacctcgaaa caatggaaga agtctggcaa
     7321 caggtaaaac ggcaggaaat tgatctctaa gggaattacg cggtcaagcg ccatttgtgt
     7381 cattttttaa atgacaagcg cttgatttgc gtcaaaaaca tttaccccaa aggggctatt
     7441 ttctcactcc tgatttcaat agtgcgctgg cgaagaggag ggataatgaa agtttgtggc
     7501 acaggtcatg ttcgggtata ctgctttccc gtcttggtta ttccatcgtc ttttcaacct
     7561 aacttctcag gttcagcatg acaacgaact atatttttgt gaccggcggg gtcgtatcct
     7621 ctctgggtaa aggcattgcc gcagcctccc tcgcagccat tcttgaagcc cgtggcctca
     7681 atgtgaccat catgaaactg gatccttaca tcaacgtcga tccaggtact atgagcccaa
     7741 tccaacacgg ggaagtgttc gttactgaag acggcgctga aaccgacttg gacctggggc
     7801 actacgagcg tttcattcgt accaaaatga gccgccgcaa caacttcacc acgggtcgta
     7861 tctactctga cgttctgcgt aaagaacgcc gcggtgacta cctcggcgca accgtgcagg
     7921 ttattccgca catcactaac gcaatcaaag agcgcgtgct ggaaggtggc gaaggtcatg
     7981 acgtagtact ggtagaaatc ggcggtacag taggtgatat cgaatccttg ccgttcctcg
     8041 aagctattcg ccagatggct gttgaaattg gccgtgagca cactctgttt atgcacctga
     8101 cgctggtgcc gtacatggca gcgtctggtg aagtcaaaac caaaccgact cagcactctg
     8161 taaaagagct gctctccatc ggtatccagc ctgacatcct gatttgtcgt tcagatcgcg
     8221 ctgtaccggc gaacgaacgt gcgaagattg cattgttctg taatgttccg gaaaaagcgg
     8281 ttatttctct gaaagacgtc gattccatct ataaaattcc gggcctgttg aaatctcagg
     8341 ggctggacga ttatatttgt aaacgattca gcttaaactg cccggaagca aatctgtccg
     8401 aatgggaaca ggttatcttc gaagaagcga atccggtaag tgaagtcacc atcggtatgg
     8461 tcggcaagta cattgaactg ccggatgctt ataaatcagt gatcgaagca ctgaaacacg
     8521 gtgggctgaa gaatcgtgtc agcgtcaaca tcaaactgat cgattcacaa gatgttgaaa
     8581 cgcgcggcgt tgaaatcctt aaaggtctgg acgcaatcct cgtacctggc ggtttcggct
     8641 atcgtggcgt agaaggcatg attacgaccg cgcgttttgc gcgtgagaac aatattcctt
     8701 atctgggcat ttgcctgggt atgcaggtgg cgttaattga ttacgctcgc catgttgcca
     8761 acatggagaa cgccaactct acagaatttg tgccagactg taagtacccg gttgtggcgc
     8821 tgattaccga gtggcgcgat gaaaacggca acgttgaagt tcgtagcgag aagagcgatc
     8881 tcggcggtac catgcgtctc ggcgcacagc agtgccagtt ggttgacgat agcctggttc
     8941 gccagctata caatgcgccg acaattgttg agcgtcatcg tcaccgttac gaagtcaaca
     9001 acatgctgtt gaaacagatt gaagatgcag gtctgcgcgt tgcgggccgt tccggggatg
     9061 atcagttggt cgagatcatc gaagttccga atcacccgtg gttcgtggct tgccagttcc
     9121 atccggagtt tacttctact ccacgtgatg gtcacccgct gtttgcaggc tttgtgaaag
     9181 ccgccagcga gttccagaaa cgtcaggcga agtaagtaaa aaagttagaa cggcaacgcg
     9241 taccttgggt acgcgttgtt tgtctggagt ttcagtttaa ctagtgactt gaggaaaacc
     9301 taatgtccaa aatcgtaaaa atcatcggtc gtgaaatcat cgactcccgt ggtaacccga
     9361 ctgttgaagc cgaagtacat ctggagggtg gtttcgtcgg tatggcagct gctccgtcag
     9421 gtgcttctac tggttcccgt gaagctctgg aactgcgcga tggcgacaaa tcccgtttcc
     9481 tgggtaaagg cgtaaccaaa gctgttgctg cggtaaacgg cccgatcgct caggcgctga
     9541 ttggcaaaga tgctaaagat caggctggca ttgacaagat catgatcgac ctggacggca
     9601 ccgaaaacaa atccaaattc ggcgcgaacg caatcctggc tgtatctctg gctaacgcca
     9661 aagctgctgc agctgctaaa ggtatgccgc tgtacgagca catcgctgaa ctgaacggca
     9721 ctccgggcaa atactccatg ccggttccga tgatgaacat catcaacggt ggtgagcacg
     9781 ctgacaacaa cgttgatatc caggaattca tgattcagcc ggttggcgcg aaaactgtga
     9841 aagaagccat ccgcatgggt tctgaagttt tccatcacct ggcaaaagtt ctgaaagcga
     9901 aaggcatgaa cactgctgtt ggtgacgaag gtggctatgc gccgaacctg ggttccaacg
     9961 ctgaagctct ggctgttatc gctgaagctg ttaaagctgc tggttatgaa ctgggcaaag
    10021 acatcactct ggcgatggac tgcgcagctt ctgaattcta caaagatggt aaatacgttc
    10081 tggctggcga aggcaacaaa gcgttcacct ctgaagaatt cactcacttc ctggaagaac
    10141 tgaccaaaca gtacccgatc gtttctatcg aagacggtct ggacgaatct gactgggacg
    10201 gtttcgcata ccagaccaaa gttctgggcg acaaaatcca gctggttggt gacgacctgt
    10261 tcgtaaccaa caccaagatc ctgaaagaag gtatcgaaaa aggtatcgct aactccatcc
    10321 tgatcaaatt caaccagatc ggttctctga ccgaaactct ggctgcaatc aagatggcga
    10381 aagatgctgg ctacactgca gttatctctc accgttctgg tgaaactgaa gatgctacca
    10441 tcgctgatct ggctgttggt actgctgcag gccagatcaa aactggttct atgagccgtt
    10501 ctgaccgtgt tgctaaatac aaccagctga ttcgtatcga agaagctctg ggcgaaaaag
    10561 caccgtacaa cggtcgtaaa gagatcaaag gccaggcata agtctgactt tatctgattt
    10621 gataatgcca gccttcgggc tggcattttt tatggtggag ttaccagcgg ccggatgcgc
    10681 cgccacttga gcctccagcg gaaccgctgt tgtctgaatg ataacttcta ccagatgctc
    10741 ttttctgaag tacacataaa caggtaaaga ccataaatat ggtgaaagta aaaaagaagg
    10801 aaaatacagc aatggaaaaa gttttatcgg agaacaggaa taaatctaaa agatggattg
    10861 ctgaaataca gactccactt ttacatgcgc gacaaaaatt actgccttga tgaaaaaacc
    10921 agaaagggaa gaacatcact gcaaataccc aaaaaatagc aaagtaataa tgatcagtgg
    10981 aagacgttga ttcactttct gaaggattag ccggatattg gtgttgtgaa gtgagttggt
    11041 tattcaaagc gattatagct aactctaatc ccttagctaa tttttgttgt ttaaatgcgg
    11101 gtatcatgtt gctacggatg atatctccag ccagagcatc ggttaccttt tcttccagcc
    11161 cataacctac ctggatgcgg acagtgcgat ccgactaggc aacaacgatc agtatcccat
    11221 cattacgttt ggcatctcct aaacgccaat tgtcaaaaac tcttgtagca tattgttcaa
    11281 ttgtctcatc tttggttgtg gaaacaatta atacagcaat ttgcacttgg gtattattga
    11341 aaatagattc taacttgtgc gtcagttctt tcttttcttg tgagtttaag ctactcgtaa
    11401 gacccgtaac ttgttgctgt aattgtggga caatagttgg ttgggctgca aca
//
LOCUS       DAZOYF010000127        11382 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZOYF010000127 DAZOYF010000000
VERSION     DAZOYF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.128"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            285..866
                     /gene="mdaB"
                     /locus_tag="ACHUOF_003111"
     CDS             285..866
                     /gene="mdaB"
                     /locus_tag="ACHUOF_003111"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR0612177.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            897..1211
                     /locus_tag="ACHUOF_003112"
     CDS             897..1211
                     /locus_tag="ACHUOF_003112"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR0612178.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1259..3151)
                     /gene="parE"
                     /locus_tag="ACHUOF_003113"
     CDS             complement(1259..3151)
                     /gene="parE"
                     /locus_tag="ACHUOF_003113"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR0612179.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3180..3761)
                     /gene="yqiA"
                     /locus_tag="ACHUOF_003114"
     CDS             complement(3180..3761)
                     /gene="yqiA"
                     /locus_tag="ACHUOF_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR0612180.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3761..4588)
                     /gene="cpdA"
                     /locus_tag="ACHUOF_003115"
     CDS             complement(3761..4588)
                     /gene="cpdA"
                     /locus_tag="ACHUOF_003115"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR0612181.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4613..5035)
                     /locus_tag="ACHUOF_003116"
     CDS             complement(4613..5035)
                     /locus_tag="ACHUOF_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR0612182.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5036..5665)
                     /gene="nudF"
                     /locus_tag="ACHUOF_003117"
     CDS             complement(5036..5665)
                     /gene="nudF"
                     /locus_tag="ACHUOF_003117"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR0612183.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5870..7351
                     /gene="tolC"
                     /locus_tag="ACHUOF_003118"
     CDS             5870..7351
                     /gene="tolC"
                     /locus_tag="ACHUOF_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR0612184.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7499..8170
                     /locus_tag="ACHUOF_003119"
     CDS             7499..8170
                     /locus_tag="ACHUOF_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR0612185.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8176..9336
                     /locus_tag="ACHUOF_003120"
     CDS             8176..9336
                     /locus_tag="ACHUOF_003120"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0612186.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9374..10162)
                     /gene="ygiD"
                     /locus_tag="ACHUOF_003121"
     CDS             complement(9374..10162)
                     /gene="ygiD"
                     /locus_tag="ACHUOF_003121"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR0612187.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10305..11078
                     /gene="zupT"
                     /locus_tag="ACHUOF_003122"
     CDS             10305..11078
                     /gene="zupT"
                     /locus_tag="ACHUOF_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR0612188.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11136..11306)
                     /gene="yqiD"
                     /locus_tag="ACHUOF_003123"
     CDS             complement(11136..11306)
                     /gene="yqiD"
                     /locus_tag="ACHUOF_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR0612189.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT         2830 a         3071 c         2804 g         2677 t
ORIGIN      
        1 atcaacatga cacgttgata ttttcatctt ttacttattc aaagccatat tttcaatccc
       61 caaaaaaacc gaaccaaacg tgtaaattat tccggtgcga gtaagaatta tgaggaatgg
      121 ctatcagtat tgtcattttc agaaaatatt tatcctgcat cggtgagtca gagctagatc
      181 agacttttgc taaattcgca aaagactttg cacattttgc taatttcaca gtaccgctct
      241 gtgacgtact atagtcgaca aacgtctcac cttgagatta aaaaatgagc aacatcctga
      301 ttatcaacgg cgcgaaaaaa ttcgcccact ccaatggtca actgaacgac accctgaccg
      361 aagtcgcgga tggcacactg cgcgacctcg ggcatgatgt ccgcatcgtt cgcgccgaca
      421 gcgactacaa tgtcaaagcg gaagtacaaa actttctctg ggctgatgtg gtgatctggc
      481 agatgccagg ctggtggatg ggcgcgccgt ggacagtgaa aaaatatatt gatgatgtat
      541 tcaccgaagg tcacgggact ctgtatgcca gcgatggtcg tacccgcaaa gatccgtcga
      601 aaaaatacgg ttccggcagc ctggtacagg acaaaaaata tatgctttct ctaacctgga
      661 acgcaccaat ggaagccttc accgaaaaag atcagttctt ccacggcgtt ggcgttgacg
      721 gtgtgtatct gccgttccat aaagcaaacc aattcctcgg tatggaaccg ctgccgacat
      781 ttatcgctaa tgacgtgata aaaatgcctg atgttccccg ctatactgaa gaatatcgca
      841 agcatcttgt ggaaattttt ggttaactag agctcaggct ttagaaggag ttaaccatgc
      901 ttaccgtaat cgcagaaatc cgtactcgtc ctggtcaaca tcaccgtcag gcggtattgg
      961 atcagtttgc taaaatcgtt ccaaccgtac tgaaagaaga aggttgccac ggctatgcgc
     1021 caatggtgga ttgcgctgct ggcgtgagtt tccagtctat ggcaccggat tctatcgtga
     1081 tgattgagca gtgggaaagc atcgcgcacc ttgaagcgca tctgcaaacc ccgcacatga
     1141 aggcgtacag cgaagccgta aaaggcgacg tgctggagat gaatatccgt attctgcagc
     1201 cagggattta atcctgcctt gtttgcccgg ccatcctgac cgggcaatgt tctttccttt
     1261 aaacctcaat ctccgccatg tcgcctttct cttgcaacca gttgcggcga tcttccgagc
     1321 gtttcttcgc cagcagcata tccatcatcg cgtcagtacg ctgatcgtct tcatcatcga
     1381 tagtcaactg caccagacgg cgagtgttcg gatcaagcgt ggtttcacgc aattgcatcg
     1441 ggttcatttc ccccagacct ttaaaacgct ggacgttcgg cttgcctttc ttgcgtttta
     1501 attgctcaag tacgccctct ttctcttctt ccgtcagcgc gtaataaacc tctttcccga
     1561 gatcaatacg gtagagcggt ggcagtgcga cgtaaacgtg accgtgtttc accaacgcgc
     1621 ggaagtgttt tacgaacaaa gcgcagagca gcgtggcaat gtgcagacca tcagagtccg
     1681 catccgcgag gatacagatt ttgccataac gaagctggct cagatcgtcg ctgtcaggat
     1741 cgataccgat cgctaccgaa atatcatgca cttcctgcga ggccagcact tcgtcggaag
     1801 aaacttccca ggtgttaagg atcttacctt tcagcggcat gatcgcctga tattcacgat
     1861 cgcgcgcctg cttggcagat ccgcctgcgg agtcaccttc cacgaggaac agttcggtac
     1921 ggttaaggcc ctgcgcggta caatcagcca gtttgccagg cagcgccggg ccgctggtca
     1981 gctttttacg caccactttt ttagccgcac gcatacgacg ctgggcgctg gaaatcgcca
     2041 tctctgccag caactccgcc gcctgaacgt tctggttcag ccacaggatg aaggcatctt
     2101 tcaccacgcc cgaaacgaat gccgcacact gacgcgaaga gagacgctct ttggtctgcc
     2161 cggcaaactg cggatcctgc attttaaccg acagaacata ggcgcagcga tcccagatat
     2221 cttccgccga cagcttcacg ccgcgcggca gaatgttgcg gtattcacag aactcacgca
     2281 tcgcgtccaa caggccctga cgcagaccat taacatgggt accgccctgc atcgttggga
     2341 taaggttgac gtagctttcg gtcagcagtt caccgccttc cggcagccac agtagcgccc
     2401 agtccacagc ttcagtatcg ccagcgaaat taccgataaa cggtttttct ggcagcgtcg
     2461 gtaaaccgtt taccgcttcc gccaggtaat cattcagacc gtcctgatag caccagcgct
     2521 gttcggtgtt gttgatctca tctttaaagg taatctcaac gccggggcac agtactgctt
     2581 tggccttcag cacatgcgtc aggcgtgaaa cagaaaaacg cgggctgtca aagaaggttt
     2641 catccggcca gaagtgcacg ctggtaccgg tattgcgttt accgcaagtg ccgacaacct
     2701 gtaaatcctg caccttttcg ccattttcaa aggcgatgtt ataaacctga ccatcgcggc
     2761 gcacgttaac ttctacgcgc ttcgacaagg cgttaactac cgaaatcccc acgccatgca
     2821 ggccgccaga gaactggtaa tttttgttag agaatttacc gcctgcatgc agacggcaaa
     2881 gaatcagttc aaccgccggt actccttctt ccgggtgaat atccaccggc atcccgcgcc
     2941 catcgtcaat cacttctaac gactggtcag catgtaagat tacatccacg cgttttgcgt
     3001 gacccgccag tgcttcatcg acactgttat caataacttc ttgccccaaa tggttagggc
     3061 gagtggtatc ggtatacatc cccggacggc ggcgaaccgg ctcaagcccg gtgagtacct
     3121 caatggcatc agcgttataa gtttgcgtca tggtttaagt tagcaattcg agttgatcgt
     3181 cagagatggt gcagaccaag aaaatcgacg atcgggttga aataatcttc gaagcccgtg
     3241 aatgcgtggt tgccgccttc tatgacagtc tggcggcagg aagcatagta cgccaccgcc
     3301 tggcggtaat ccagcacttc atctcccgtc tgttgcagca gccagatcaa atccggcgct
     3361 tccagcgggt caatctgcat gactttgaga tcgtaaatat ggcgtgactc tagcacatat
     3421 tgctgcccgg tgtaggggtt ctcgttctga ccgagatagt ccgtcagcaa ttcaaacggg
     3481 cgtaccgccg ggtttaccac cactgcgggc agcataaaac attgcgacaa ccaggtggcg
     3541 taatatcccc ccagtgacga accgacaata cccagcgaat cgccgccatg ttccaggaca
     3601 atggattcca gcagttctgc cgcgtcggaa ggatacggcg gcaactgcgg aatgatcatc
     3661 tcaacgtcag ggtgatgttc cgccagccag ttttttaaca agctcgcttt tgcagagcgc
     3721 ggcgagctgt tgaaaccgtg taaataaaga agcgtagaca tcagtagcct tctgaagcgg
     3781 tatcaggttg gaaacgtgtg tccgccaggc gatgcacctc ggtggtcagc gtgccatcag
     3841 catgtaactc gagagtacgc cagccgggcg cgatagtatc cagcgtaaag ttggaacagt
     3901 gcggcttaaa ctgcacacag gtcgacggcg ttgccagcag gcggcgaccg ttccagtcga
     3961 gatccagctc ctgatgaata tgaccgcaca gcaagtattt gacgtgcgga aacttcgcca
     4021 gcacggtatc cagttcgccc gcgttacgca gactgtgttg atcgagccaa ctacaacccg
     4081 caggtagcgg atgatgatgc agcagcagca acgtatggcg ttctggcgca tcggccagtt
     4141 tacgttccag ccactcaagc tgaaactcgc tcagctcacc gtgcggcacg ccaaacacct
     4201 ggctatccag caacaggatt tgccattgct caccaataaa cacccgcttc gccggggaga
     4261 tacccgcatc ctgtaacgcg ctgtacatcg cgggctggaa atcgtggttg cccggcagcc
     4321 agacgcaggg cgcacgaaaa cttgcgatgc cttcagcgaa atgctgatag gccgcagagg
     4381 attgatcctg cgctaaatca cctgtcgcga caatcaggtc gaattcgtgc tggtgtggac
     4441 gaatcgcctc cagcaccgcc tggtagcttt cccaggtgtt tacccctaac agggcttcgt
     4501 gcttttgtgc aaacaggtga gtatcggtaa tttgtaaaat ccttcctctg gcctcaccag
     4561 ccagaggaag ggttaacagg ctttccaaat ggtgtcctta ggtttcacga cgctagtaaa
     4621 ccggaatcgc catcgctcca tgtgctaaac agtatcgcaa ccagtccgct aaaaactggt
     4681 taatttgatg cttttcgtcg cgttgatgca actttttatt aggataatca taccgcgctt
     4741 tgaagcgaaa aatctgctgg cttgaacaca cttcagccac catcgcgtca tgatacagac
     4801 gcaccgtcat tgacggaagg ctccagtaac tgatcgcggg cgcagtctgt tctattgtca
     4861 ccagggtagt gtatcgggtc gattccacaa tcgtcagccg atattgtgcg tttgccacct
     4921 gatagcttac agtttcgccg ggtgcgtcat tgcgcggtaa caaacggcgc aattgtgaaa
     4981 agttcatctc gcacaggcgc atcatttcag gaaagtcagg tgtgtaacgc ttcatttatg
     5041 cccactcatt ttttaacgct tgatgatgca gctgcagcca ttgcaaagcg atgaccgacg
     5101 ctgcgttgtc gattttcccc tcttctaccc actggtatgc ctgttcccgg cttaccacat
     5161 gaacgcgaat atcttcgttt tcatcagcca gaccatgaat accgcttgcg gtcgtggcgt
     5221 ccacttcgcc caccataatt gacgaacgct cactggtgcc ccccgggctt gccaggaaac
     5281 ttaacaccgg tttggtccgt ttgactatca gtcccgcctc ttcaatcgct tcgcgacggg
     5341 caacatcttc cacactttca ccctcttcaa tcatcccggc aaccatctcc agtagccagg
     5401 gggtttcgct ggtgtcgtac gcggcaatcc gaatctgctc aatcagcaca acttcatcac
     5461 gcactgggtc aaagggtagc aagactgcgg cgtgaccgcg ctcaaaaatt tcccgccgta
     5521 cctcatgact catttgcccg ttgaatagac gatgacgaaa tctataaaga tctaatgaaa
     5581 aaaagccgcg ataaagtgtt tctcgtgcaa taatttctac atcgtttttg ccaaatgtaa
     5641 cgggcaggtt gtctggctta agcattgtta atgtcctagc actaatagtg aattaaatgt
     5701 gaatttcagc gacgtttgac tgccgtttga gcagtcatat gttaaattga ggcacattaa
     5761 cgccctatgg cacgtaacgc caaccttttg cggtagcggc ttctgctaga atccgcaata
     5821 attttacagt ttgatcgcgc taaatactgc ttcaccacaa ggaatgcaaa tgaagaaatt
     5881 gctccccatt cttatcggcc tgagcctttc tgggttcagt tcgttgagcc aggccgagaa
     5941 cctgatgcaa gtttatcagc aagcacgcct tagtaacccg gaattgcgta agtctgccgc
     6001 cgatcgtgat gctgcctttg aaaaaattaa tgaagcgcgc agtccattac tgccacagct
     6061 aggtttaggt gcagattaca cctatagcaa cggctaccgc gacgcgaacg gcatcaactc
     6121 gaacgcgacc agtgcgtccc tgcagttaac tcaatccatt tttgatatgt cgaaatggcg
     6181 tgcgttaacg ctgcaggaaa aagcagcagg gattcaggac gtcacatatc agaccgatca
     6241 gcaaaccttg atcctcaaca ccgcgaccgc ttatttcaac gtgttgaatg ctattgacgt
     6301 tctttcctat acacaggcac aaaaagaagc gatctaccgt caattagatc aaaccaccca
     6361 acgttttaac gtgggcctgg tagcgatcac cgacgtgcag aacgcccgcg cgcagtacga
     6421 taccgtgctg gcgaacgaag tgaccgcacg taataacctt gataacgcgg tagagcagct
     6481 gcgccagatc accggtaact actatccgga actggcggcg ctgaatgtcg aaaactttaa
     6541 aaccgacaaa ccacagccgg ttaacgcgct gctgaaagaa gccgaaaaac gcaacctgtc
     6601 gctgttacag gcacgcttga gccaggacct ggagcgcgag caaattcgcc aggcgcagga
     6661 tggtcactta ccgactctgg atttaacggc ttctaccggg atttctgaca cctcttatag
     6721 cggttcgaaa acccgtggtg ccgctggtac ccagtatgac gatagcaata tgggccagaa
     6781 caaagttggc ctgagcttct cgctgccgat ttatcagggc ggaatggtta actcgcaggt
     6841 gaaacaggca cagtacaact ttgttggtgc cagtgagcaa ctggaaagcg cacatcgtag
     6901 cgtcgtgcaa accgtacgtt cctccttcaa caacattaat gcttctatca gtagtattaa
     6961 cgcctacaaa caagccgtag tttccgcgca aagctcatta gacgcgatgg aagcgggcta
     7021 ctcggtcggt acgcgtacca ttgttgatgt gttggatgcg accaccacgc tgtacaacgc
     7081 caagcaagag ctggcgaatg cgcgttataa ctacctgatt aatcagctga atattaagtc
     7141 agccctgggt acgttgaacg agcaggattt gctggcactg aacaatgcgc tgagcaaacc
     7201 ggtttccact aatccggaaa acgttgcccc gcaaacgccg gaacagaatg ctattgctga
     7261 tggttatgcg cctgatagcc cggcacccgt cgttcagcaa acatccgcac gcactaccac
     7321 cagtaacggt cataaccctt tccgtaactg atgacgacga cggggcttcg gccccgtctg
     7381 aacgtaaggc aacgtaaaga tacgggttat ctgccgcatt cttccccctt ctcgcttcaa
     7441 tttcgaccag ccatcctcta ttctgatggg tatttaccac tggtcccgga agacaaaaat
     7501 gaaacggaca aaatccatac gccacgcatc gttccgcaaa aactggagcg cacgccatct
     7561 gacaccagtc gctctcgcgg ttgccactgt ttttatgctg gctggctgtg aaaagagtga
     7621 tgaaacagtg tctctctatc aaaatgctga cgactgttca gctgcaaacc caggcaaaag
     7681 cgccgaatgt accaccgcgt acaacaatgc gctgaaagaa gccgaacgta ctgcgccgaa
     7741 atacgccacc cgtgaagact gtgttgctga atttggtgaa ggtcagtgtc agcaagcacc
     7801 agcccaggct ggcatggcac cagaaaacca ggcgcaggct cagcaatcca gcgggagttt
     7861 ctggatgccg ctgatggccg gttacatgat ggggcgtctg atgggcggcg gcgcgggatt
     7921 tgcacagcag ccgctgttct cctcgaaaaa cccggccagt ccggcttacg gtaaatatac
     7981 cgacgcgacg ggtaaaaact atggcgcagc ccagccaggc cgcaccatga ccgtaccgaa
     8041 gacggcaatg gcaccaaaac cggcgaccac cactaccgtt acccgtggcg gttttggtga
     8101 atctgttgcc aaacaaagca ctatgcagcg tagcgcaacc ggcacctctt ctcgttcaat
     8161 gggtggctga taccgatgga aagagtcagt attaccgagc gcccggactg gcgtgagaaa
     8221 gcccacgaat acggtttcaa ttttcacacc atgtacggcg agccgtactg gtgtgaagat
     8281 gcttactaca agttgaccct cgcccaggtt gaaaagctgg aagaagtcac cgccgaactg
     8341 caccagatgt gcctgaaagt ggtggaaaaa gtgatcgcca gcgatgagct gatgaccaaa
     8401 ttccgcatac caaaacacac ctggagtttt gtgcgtcaat catggctgac tcaccagcca
     8461 tcgctttatt cgcgtcttga tctggcgtgg gatggcactg gcgaacctaa acttctggaa
     8521 aataacgccg atacgccaac gtcactatac gaggcggcgt tctttcagtg gatctggctg
     8581 gaagatcagc ttaacgccgg taacttgccg gagggcagcg accagtttaa cagtctgcaa
     8641 gaaaaactga tcgatcgctt cgttgagctg cgtgaacagt atggcttcca gttgctgcat
     8701 ctcacctgct gtcgcgacac ggtggaagat cgcggaacca ttcagtattt gcaggactgc
     8761 gcaaccgaag ctgaaatcgc caccgagttc ctctacatcg acgagatagg tttaggcgaa
     8821 aaaggccagt tcacggattt acaggatcag gtgatttcta acctgttcaa gctttatccg
     8881 tgggaattta tgctgcgcga aatgttctcc accaagctgg aagatgctgg cgtacgctgg
     8941 ctggaaccgg cgtggaagag cattatctcc aacaaggcac ttctaccgct actgtgggag
     9001 atgttcccga atcacccgaa cctgctgccc gcttattttg cggaagatga tcatccgcaa
     9061 atggaaaaat atgtggttaa accgatcttc tcccgtgaag gcgcaaacgt gtcgatcatt
     9121 gagaacggca aaaccattga agcagcggaa ggtccgtatg gcgaagaagg gatgattgtt
     9181 cagcaattcc acccgttacc gaaattcggc gacagctata tgctgattgg tagctggctg
     9241 gtgaacgatc aacctgccgg aattggcatt cgtgaagacc gtgcgttgat cacccaggat
     9301 atgtctcggt tttatccaca tatttttgtt gaataagcca cgataccgga tggcactcgc
     9361 catccggtaa ttgttagcct atctgcaccg acagcatact taaactgccc atttctatac
     9421 catcgaccgg aatggtaatt ggctcctgcc catcccacac acctaacaca tacaacaacg
     9481 gcagatagtg ctctggcgtt gggttcgata acgcgccacc ttcatggtcg agataattca
     9541 ccagaggatg ctgctctacc ggcccttgcc acgtcagatt cgctttcaca tactcattaa
     9601 acgacgtcgc ccacggatac ggtgaactat caccgtgcca cttcactgtg cgcaggttat
     9661 gcaccacgtt accgctggcg accaacatta ttccttcatc acgcagcgcc gcgagtttgc
     9721 gccccatttc gaaatgccag gcggcaggtt tgctactgtc gatactcaac tgcaccatag
     9781 ggatatcggc gtcaggatac atcttaatca gcacgcccca cgagccgtgg tcaaagcccc
     9841 aggcttcttt atccagcgtc accgggatcg gcgctaacag ctcaaccaga cgctgtgcca
     9901 gcgcaggcga acccggagca ggataatgcg tatcgtacag cgcctgcggg aagccaccaa
     9961 agtcatgaat cgtgggcggc gtctccatcg cggtcactcc tgttccacgg gtaaaccagt
    10021 gagccgaaac caccacaatc gcttgcgggc gtggcaatgt catccccaac ttctgccagc
    10081 tgcgggtata caaattatct tccagcacgt tcatcggact accgtgacct aaaaacaatg
    10141 ctggcatacg tgttgaagac atgatgatat ccttaactaa aggtgtcatt ttgatatcct
    10201 cacaatacgc ttgttcggcg gagtaagaac ccggataaca atgatgatga tcatcagtta
    10261 ttttgacgat ctgcctgaag gtgaagattt ataaggagtt gtcgatgtca gtacctctca
    10321 ttctgaccat actggcgggg gcagccacgt ttattggcgc gtttctcggc gttctcggac
    10381 aaaaaccctc gaaccgctta ctggcgtttt cgctaggttt tgcggcgggg atcatgttgc
    10441 tcatctcatt aatggaaatg cttcctgccg cactagcagc tgaaggaatg tcgccggtgt
    10501 tgggttatgg aatgtttatc ttcggtctgc ttggctactt tggcctggac cgcatgttgc
    10561 cgcatgcgca tccgcaggat ttaatgcaaa aatcggtgca gctgttgcca aaatcgatca
    10621 agcgcacagc cattctgctc actctcggca tcagtctgca taacttcccg gaagggattg
    10681 ccacctttgt cacggcgagc agcaacctgg agctgggatt tggcatcgca ctggccgtcg
    10741 cgttgcacaa tatccctgaa ggtctggcag tggcaggccc ggtttatgcg gcaacggggt
    10801 ctaaacgcac cgcgattctg tgggcgggga tttctggact agcagaaatt cttggtggtg
    10861 tgctggcgtg gttaatcctc ggtagcatga tttccccggt ggtcatggcg gcaatcatgg
    10921 cggcggttgc aggaattatg gtggcgctct cggttgatga attaatgccg ctcgccaaag
    10981 agattgaccc taataataac cccagctatg gcgtactatg tggaatgtca gtgatgggat
    11041 tcagtttagt gctgctacaa acggcgggaa ttggttaaaa acaacatcag aagttgtcca
    11101 gcacatggac aacttctgcc agcttcacga aagccttatt ctaccttttc ggctgtctct
    11161 tctctcgtac tgtttaagta tttgttccgc gcttcaagca gtgcgtctct gtcctggcga
    11221 aacgcccgac aataacgttt caaatgcagg aaataaccca ccacaaccag agcaatcaat
    11281 acaatccagt accaggcgat aaacatcctt ttacccttaa tatttaacaa tactaaacat
    11341 ttagcgcata aatttcacat atcctttttc ggatatattc at
//
LOCUS       DAZOYF010000128        11327 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZOYF010000128 DAZOYF010000000
VERSION     DAZOYF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.129"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHUOF_003124"
     CDS             complement(<1..209)
                     /locus_tag="ACHUOF_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0612190.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHUOF_003125"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHUOF_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR0612191.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHUOF_003126"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHUOF_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR0612192.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHUOF_003127"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHUOF_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR0612193.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHUOF_003128"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHUOF_003128"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR0612194.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHUOF_003129"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHUOF_003129"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR0612195.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHUOF_003130"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHUOF_003130"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR0612196.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ACHUOF_003131"
     CDS             complement(4598..5320)
                     /locus_tag="ACHUOF_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0612197.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHUOF_003132"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHUOF_003132"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0612198.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="ACHUOF_003133"
     CDS             6520..6738
                     /locus_tag="ACHUOF_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR0612199.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHUOF_003134"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHUOF_003134"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0612200.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="ACHUOF_003135"
     CDS             complement(7472..7672)
                     /locus_tag="ACHUOF_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR0612201.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHUOF_003136"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHUOF_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHUOF_003137"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHUOF_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR0612202.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..>11327
                     /locus_tag="ACHUOF_003138"
     CDS             10101..>11327
                     /locus_tag="ACHUOF_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0612203.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQK"
BASE COUNT         2790 a         3099 c         2721 g         2717 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgctaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaa
//
LOCUS       DAZOYF010000129        11275 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZOYF010000129 DAZOYF010000000
VERSION     DAZOYF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.130"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            67..201
                     /locus_tag="ACHUOF_003139"
     CDS             67..201
                     /locus_tag="ACHUOF_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR0612204.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTASGKGKWKIITA
                     "
     gene            180..284
                     /gene="speFL"
                     /locus_tag="ACHUOF_003140"
     CDS             180..284
                     /gene="speFL"
                     /locus_tag="ACHUOF_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR0612205.1"
                     /translation="MENNNRLMPHIRRTTHIMKFTHRNSFDFHFFNAR"
     gene            595..2792
                     /gene="speF"
                     /locus_tag="ACHUOF_003141"
                     /pseudo
     CDS             595..2792
                     /gene="speF"
                     /locus_tag="ACHUOF_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2789..4108
                     /gene="potE"
                     /locus_tag="ACHUOF_003142"
     CDS             2789..4108
                     /gene="potE"
                     /locus_tag="ACHUOF_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR0612206.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4164..5804)
                     /gene="pgm"
                     /locus_tag="ACHUOF_003143"
     CDS             complement(4164..5804)
                     /gene="pgm"
                     /locus_tag="ACHUOF_003143"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR0612207.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5830..6375)
                     /gene="seqA"
                     /locus_tag="ACHUOF_003144"
     CDS             complement(5830..6375)
                     /gene="seqA"
                     /locus_tag="ACHUOF_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR0612208.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6560..7324
                     /gene="ybfF"
                     /locus_tag="ACHUOF_003145"
     CDS             6560..7324
                     /gene="ybfF"
                     /locus_tag="ACHUOF_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0612209.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7464..7757
                     /gene="ybfE"
                     /locus_tag="ACHUOF_003146"
     CDS             7464..7757
                     /gene="ybfE"
                     /locus_tag="ACHUOF_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR0612210.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7897..8427
                     /gene="fldA"
                     /locus_tag="ACHUOF_003147"
     CDS             7897..8427
                     /gene="fldA"
                     /locus_tag="ACHUOF_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR0612211.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8637..8723
                     /gene="uof"
                     /locus_tag="ACHUOF_003148"
     CDS             8637..8723
                     /gene="uof"
                     /locus_tag="ACHUOF_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR0612212.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8716..9162
                     /gene="fur"
                     /locus_tag="ACHUOF_003149"
     CDS             8716..9162
                     /gene="fur"
                     /locus_tag="ACHUOF_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR0612213.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9246..9572)
                     /gene="chiQ"
                     /locus_tag="ACHUOF_003150"
     CDS             complement(9246..9572)
                     /gene="chiQ"
                     /locus_tag="ACHUOF_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR0612214.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9609..11015)
                     /gene="chiP"
                     /locus_tag="ACHUOF_003151"
     CDS             complement(9609..11015)
                     /gene="chiP"
                     /locus_tag="ACHUOF_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFR0612215.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2760 a         2775 c         2802 g         2938 t
ORIGIN      
        1 tataaaacac gaaattacaa catttaagta acaaaacagc atctaatatg gcgatattca
       61 taattaatga tttattatct ggggtatgat cgttttttgt tgatcttctt cacagattat
      121 agccatttca tggatagaat aactctacct tcaactgaca cagcaagcgg taaaggtaaa
      181 tggaaaataa taaccgctta atgcctcata taaggcggac aacccatatc atgaagttta
      241 cccatcgtaa tagttttgac tttcatttct ttaatgcccg ctagtcttct gactaaaggg
      301 caccccaacg tacaggtctc cctgacttta agcattacag gttaatacct gtattcctcg
      361 gtgctcatat actgctaacc cttttaaact ctaaataatt cgagtcgcag cacttgcaac
      421 ttgaggtatg acgagtatag ccagttaccg ggctggtctg ggttattgca tctgcaaaaa
      481 gcaaactact gatttattta tcagcggtgg agctttgctt tttttccgac gtgatcgatt
      541 tctcctttga gaaattgagg acctgctatt acctgaaata aagagatgaa taaaatgtca
      601 gaattaaaaa ttgccgttag ccgttcttgc ccggattgtt tttccactca tcgtgcatgc
      661 gtgaatattg acgaaagtaa ttatattgac gttgccgcca ttattttatc agtcaatgat
      721 gttgaacgtg gaaaactcga taaaatagac gctactggct atggcattcc tgtttttatt
      781 gcaacggaaa atgaagaacg tgtaccagca gaatatctgc cacgtatttc tggtgtcttc
      841 gagcattgcg aatcacggaa agagttttat ggacgtcagt tagaaaccgc tgctagccat
      901 tatgaaacgc aactgcgccc gcccttcttc cgcgcactgg tcgattatgt caatcagggc
      961 aatagcgctt ttgactgccc gggtcatcag ggaggtgagt ttttccgtcg tcatccggca
     1021 gggaatcagt tcgtggagta cttcggagaa acgctgttcc gttccgactt atgcaacgca
     1081 gacgtagcaa tgggcgatct gctgattcac gaaggcgcgc catgcattgc acagcaacat
     1141 gcggcaaaag tgtttaatgc cgataaaaca tacttcgttc taaatggcac ttcatcttct
     1201 aacaaagtgg ttttaaacgc cctgctaaca ccgggtgatc tggtgctgtt tgatcgcaat
     1261 aaccacaaat ctaaccacca cggagcgttg ctacaggctg gtgcaacacc ggtttatctg
     1321 gaaacggcac gtaacccgta tggctttatc ggtggcattg atgcgcactg ttttgaagaa
     1381 agttacctgc gtgagctgat cgcggaagtc gcaccgcagc gggcaaaaga ggctcgtcct
     1441 ttccgcctcg ctgtgattca gttaggcacc tacgacggta cgatttataa cgcccgccag
     1501 gtggtggata aaattggtca tctgtgtgac tacattctgt ttgactccgc atgggttggc
     1561 tatgagcaat ttatcccgat gatggctgac tgttcgccgc tgttgctgga tcttaatgag
     1621 aacgatccgg gtattctagt tacgcaatcc gtacataaac agcaggctgg tttttctcaa
     1681 acttcacaaa tccataaaaa agacagccac atcaaagggc aacagcgtta tgtaccgcac
     1741 aaacgcatga acaacgcctt tatgatgcac gcctccacca gcccgttcta tccgctgttt
     1801 gccgcactgg atatcaacgc caaaatgcat gaaggtgtca gcggtcgtaa tatgtggatg
     1861 gattgtgtgg taaatggcat taatgcccgc aaactgatcc tcgataactg tcagcatatt
     1921 cgtccgttcg tacctgaact ggtggatggt aaaccctggc agtcgtatga aacagcgcaa
     1981 attgcggttg atctgcgctt cttccagttt gtaccagggg aacactggca ttcttttgaa
     2041 ggctatgcag agaatcaata ctttgtcgat ccttgcaaac tgttgctgac aaccccaggt
     2101 attgatgcac gtaacggcga atatgaagcg ttcggtgtac ccgcgacgat tcttgctaac
     2161 ttcctgcgcg aaaatggcgt agtgccggaa aaatgcgatc ttaacgccat cctcttcctg
     2221 ctgactccgg cagaagatat ggccaaactt cagcaacttg tgccctgctg gtacgcttcg
     2281 aaaaactgct tgagtccgac gcgccattag cagaagtgct gccttccatc tacaaacagc
     2341 atgaagagcg ctacgccggt tataccctgc gtcagttgtg ccaggaaatg catgatttgt
     2401 atgcccgcca caacgtgaaa caactgcaaa aagagatgtt ccgcaaagcg cacttcccac
     2461 acgtcagcat gaatccgcaa gaagccaact acgcctattt acgcggtgaa gtggagctgg
     2521 ttcgtctacc ggatgccgaa ggtcgtatcg ctgccgaagg ggcgcttcct tatcctccgg
     2581 gtgtgctgtg tgttgttccg ggtgaaatct ggggtggtgc tgttttgcgt tacttcagcg
     2641 ctctggaaga agggatcaac ctgctgccag gttttgcacc ggagctgcag ggtgtctata
     2701 tcgaagaaca tgatggtcgt aagcaagttt ggtgctatgt catcaagcct cgtgatgcgc
     2761 aaagcaccct gttgaaaggg gaaaaattat gagtcaggct aaatcgaaca aaatgggcgt
     2821 cgttcagtta accatactga cgatggtcaa catgatgggc tccggtatca tcatgctgcc
     2881 gacaaagctt gccgaagttg ggacaatttc aattatctcc tggctggtga cagccgtagg
     2941 ctcaatggca ctggcatggg cattcgcaaa atgcggtatg ttcagccgta aatcaggtgg
     3001 tatgggcggt tatgccgagt atgctttcgg taaatccggt aactttattg cgaactatac
     3061 ctacggcgtc tcactgctga ttgctaacgt cgcgattgct atttcggcgg ttggttacgg
     3121 caccgaactg ctcggcgcga gtttgtcgcc agtgcagatt ggtcttgcga ccatcggggt
     3181 gctgtggatt tgtaccgtgg ctaactttgg tggtgcgcgc attaccgggc aaatcagtag
     3241 cattaccgtg tgggggggca ttattccggt ggtaggcctg tgcattatcg gctggttctg
     3301 gtttagcccg acgctttacg ttgattcctg gaatccgcat catgcaccgt tcttcagtgc
     3361 agtaggttct tccatcgcta tgacgctgtg ggcttttctt ggtctggagt ctgcgtgtgc
     3421 gaatactgat gtagtggaaa acccggaacg taatgtgcca atcgcggtac tcggcggtac
     3481 gttaggtgcg gcggtgattt atatcgtctc caccaacgtg attgcaggga ttgtgcctaa
     3541 tatggagctg gcaaattcaa cggcaccatt tggcctggcc ttcgcacaga tgtttacacc
     3601 agaagtgggt aaagtcatta tggcgctgat ggtgatgtcc tgctgcggtt cgctgcttgg
     3661 ctggcagttc accattgccc aggtgtttaa atcttcatct gatgaaggct acttccctaa
     3721 aattttctcc cgtgtaacca aagtggatgc accggtgcag ggaatgttga ccattgtgat
     3781 tatccagagc gggttggcgc tgatgacgat tagcccgtcg ctaaacagtc agttcaacgt
     3841 gctggttaac ctggccgtgg tgaccaatat cattccgtat attctgtcaa tggcggcatt
     3901 agtcattatt cagaaggtcg ctaatgtgcc cccctcaaaa gcgaaagttg caaactttgt
     3961 tgcttttgtt ggcgcgatgt atagctttta tgcgctgtac tcatccgggg aagaagccat
     4021 gctgtacggt tccatcgtga ccttccttgg ttggacactg tatggtctgg tatcaccacg
     4081 ctttgaactg aaaaataaac acggttaaaa aagcaaagta aaaaagggcg atcttgcgac
     4141 cgctcttttt ttattaaatg tgtttacgcg tttttcagaa cttcgctaac aatctcaacc
     4201 gcttctttct caatctgctt gcgatgttct tcaccgagga agctttcgca gtagatctta
     4261 tatgcgtctt ccgtgcctga cggacgcgcg gcgaaccagc cgttgtcagt catcactttc
     4321 agaccgccaa tagaagcacc gttgcccgga gcagcagtca ggcgcgcggt gatcgggtca
     4381 cctgccaggg tgctggcgct caccatttcc ggagacagct tagacagcgc cgctttttgt
     4441 gcggaagtcg cagctgcctg caaacggttg tagctcggcg caccaaagcg ttttgccagt
     4501 tcgttgtagt gttcctgcgg gttcttaccg gtgacagcgg tgatttccgc cgccagcaga
     4561 cacataatga tgccgtcttt gtcggtggac cacggcgtgc cgtcgaaacg caggaaggaa
     4621 gcccctgcgc tctcttcgcc gccaaagccg aagctgccgt cgaacagacc atcaacaaac
     4681 catttgaaac ctaccggtac ttctaccagc ttacggccca agtcattgac cacacggtcg
     4741 atcatcgcag aagaaaccag cgttttaccg acagcaacat ctttgcccca ctgcggacga
     4801 tgctggaaca ggtagttgat cgccaccgcc aggtagtggt tcggattcat caaacctgcc
     4861 ggagtgacga taccgtgacg gtcataatcc gggtcgttag caaacgccag atcgaactta
     4921 tcacgcagtg ccagcagacc cgccatcgca cactcggagg agcagtccat acggatcgca
     4981 ccgtctttat cgaggtgcat aaagcggaag gtttgatcga cctgatcgtt aacgatggtc
     5041 aggttgaggt tgtaatactc gccaatgcgc ttccagtatt cgataccgga accgcccaac
     5101 ggatcaacgc ccagcgtcag gcccgctttc tgaatcgccg ccatatcaac gatatcggcc
     5161 agcccttcca cgaacggctg caccagatcc tgctctttca catagccgga tgccatcgct
     5221 tcgtcgaggg agatacgctt cacgcctttc aggccatcgg ccatcagtgc gttggccttg
     5281 tcttccacca ctttggtgac gttagtatca gccgggccac catttggcgg attgtacttg
     5341 ataccaccat cttccggcgg gttatgggac ggtgtaatca cgataccgtc tgccagcggg
     5401 ccaccttttt tattgtgaac caggatggca ttggaaacgg caggcgtcgg agtgaagcca
     5461 ttgttttcct gcacaatgac atcaacgccg ttcgctgcca gcacttccag tacggaaatg
     5521 aacgcaggct cggacagggc gtgagtatct ttacccacat agcaagggcc agtgatgccg
     5581 tttttcgcac gttcttcagc aattgcctga gcgatcgcca gaatgtgcgg ctcgttaaag
     5641 ctgtggcgcg ctgcactgcc acggtgaccg gaagtaccga atttcaccgc gtgctccgca
     5701 ttccctgctt ctggtttcag tacataatat tgcgccgtca gttgggcgac gttaatcaaa
     5761 tcactctgtt gtgcaggttg gcctgcacga ttgtggattg ccattgcttt gtcctttgtc
     5821 tgcaacgttt tagatagttc cgcaaacctt ctcaatcaat tccgccggga attgcatcga
     5881 ctgcatgatg tgttcgatca tgctgcattt acggccggtg ttggtgttgg tgatcaccca
     5941 atacggcgtg cctggcacat gtttcggctt ggtctgatta ccatttttca gcagcgtttg
     6001 ttcatctgcc gcaaagtaca cgcgtgtacg accgtgcaac gattccgttg cttcggcaaa
     6061 cgcctgggcg tcaagagaat atagtgtaga caacagcagc ataaagcgat tgaccgctcg
     6121 cttttgctct gcgtattcgt ccgaaagcag aagttcacgc attgcgcgaa ccttgtcttt
     6181 aatcgttttg accggcttcg cttcgacgat agcaggtgac gcaacgcgaa cctctttcgt
     6241 caccggagca gcaggctgtg atgcggcgga aaatttcaac atacgccgta aaatgtcgga
     6301 tgcgctctcg ccgatatgct tagtgtggct ggcaatatag ctgtagagtt catcatcaac
     6361 ttcaatcgtt ttcatcttaa tccagtgcag tgtcttagct gaatacaagt cgccaagagt
     6421 atatgaataa atcccggcag cggatagcgt caagcctggc ttgcagaaaa actcggaata
     6481 aaccttattt gttgcagccc tgtggcacaa tggtcaacat aataccctaa cccgacaacg
     6541 cgaaaaaaag aactttgcca tgaaattaaa tatccgcgcg caaactgcac aaaaccagca
     6601 caataattct cccatcgttc ttgtccatgg tctgtttggt agcctcgaca accttggcgt
     6661 actggctcgc gatctggtaa acgatcacaa tatcatccag gttgatatgc gtaaccacgg
     6721 tctttcaccg agagagccgg taatgaatta cccggcgatg gcgcaggatc ttgttgatac
     6781 cctggatgca ctgcagatcg acaaagcaac atttatcggt cactccatgg gcggtaaagc
     6841 ggtaatggca cttactgcac tggcccccga tcgcatcgat aaactggtgg cgatcgatat
     6901 cgcaccggtc gactatcacg tacgccgtca tgatgagatt ttcgcagcta tcaacgcggt
     6961 cagtgaatcg gacgcacaaa ctcgccagca agcagcagca ataatgcgcc agcatcttaa
     7021 tgaagaaggg gtgattcagt ttctgttgaa atcttttgtt gacggggagt ggcgctttaa
     7081 cgtgccggta ttgtgggatc agtatccgca tattgtgggt tgggagaaaa tcccggcatg
     7141 ggatcaccct gccctgttta tccctggcgg caattctccg tatgttagcg agcagtaccg
     7201 tgatgattta ctggcacaat ttccacaggc acgggcgcat gtgattgcag gcgcgggtca
     7261 ctgggtccat gctgaaaaac cggatgcggt attacgcgct atccgtcgct atctcaatga
     7321 ttaacgcaac tgattaaaaa cccagcgtcc gcgtcgcttt ccgcgcgtgg gcgttggcgt
     7381 gactcatcgc gctgatgtat tatggcgcgc tatccatccg ggcagaggcc tggctgattg
     7441 tttcccccga agtcaccaag atcatggcca aagaacaaac ggaccgtacg acattagatc
     7501 tgttcgcgca cgagcgtcga ccgggacgac cgaaaactaa tccgctttcg cgcgatgaac
     7561 agctgcgtat taataaacgc aaccagctaa aacgcgacaa agtacgtggc cttaagcgtg
     7621 tcgaactgaa gctgaacgcg gaagctgtcg aggcgctgaa cgagctggcg gaatcgcgta
     7681 atatgagccg tagcgaactg atcgaagaga tgttaatgca gcaactggcg gcgctgcgta
     7741 gtcagggcat cgtttaaatt tccactttca tgtagcacag tgtgcagtcc tgctcgtttg
     7801 ctgattccgc aagactgcct gttctgctat gattgccttt atccgtgggc aattttccac
     7861 ccccatttca ataagtttca agaggttatt tcactcatgg ctatcactgg catctttttc
     7921 ggcagcgaca ccggtaatac cgaaaatatc gcaaaaatga ttcaaaaaca gcttggtaaa
     7981 gacgttgccg atgtccatga cattgcaaaa agcagcaaag aagatctgga agcttacgac
     8041 attctgctgc tgggcatccc aacctggtat tacggcgaag cgcagtgtga ctgggatgac
     8101 ttcttcccga ctctcgaaga gattgatttc aatggtaaac tggttgcgct gtttggttgt
     8161 ggtgaccagg aagattacgc cgaatatttc tgcgacgcat tgggcaccat ccgcgacatc
     8221 attgaaccgc gcggtgcaac catcgttggt cactggccaa ctgcgggcta tcatttcgaa
     8281 gcatcaaaag gtctggcaga tgacgaccac tttgtcggtc tggctatcga cgaagaccgt
     8341 cagccggaac tgaccgctga acgtgtagaa aaatgggtta aacagatttc tgaagagttg
     8401 catctcgacg aaattcttaa tgcctgatgt gatgcggcgt agactcatgt ctacgccgta
     8461 ttaatagata atgccaatca aaataattgc tacaaatttg taacttttgc tgttgtacct
     8521 atacaatgtc ccagtgttca agtggccttg ccgttgtaaa tgtaagctgt gccacgtttt
     8581 tattaacaat atttgccagg gacttgtggt tttcatttag gcgtggcaat tctataatga
     8641 tacgcattat ctcaagagca aattctgtca cttcttctaa tgaagtgaac cgcttagtaa
     8701 caggacagat tccgcatgac tgataacaat accgccctaa agaaagctgg cctgaaagta
     8761 acgcttcctc gtttaaaaat cctggaagtt cttcaggagc cggacaacca tcacgtcagt
     8821 gcggaagatt tatacaaacg tctgatcgat atgggtgaag aaattggtct ggctacggta
     8881 tatcgcgtac tgaaccagtt tgacgacgct ggtatcgtca cccgccacaa ttttgaaggc
     8941 ggtaaatccg tatttgaact gacacagcaa catcaccacg atcacctgat ttgcctcgac
     9001 tgcggcaagg ttatcgaatt tagtgatgat tccatcgaag cgcgtcagcg tgaaattgcc
     9061 gcaaaacatg gcattcgcct gactaaccac agtctctatc tttacggtca ctgtgccgaa
     9121 ggcgattgcc gcgaagatga gcacgcgcac gaaggcaaat aagccagcct gaacgagaaa
     9181 agccaacctg cgggttggct tttttataca agggaaataa gaaaagcgag agttacagct
     9241 ctcacttatt tgttattact gcgtatttcc tgccagacct tatcacaatc ggctttcacc
     9301 gcctgatcat ttccggtttg cgttgcacgc agacactgct gataatccag tacgcgtaca
     9361 ctttcctggt cggcaaactg ctgatgttgt ttctctttct tcagcacgtt taacacgctc
     9421 tggcaggctt caattttttc tggcgaacct tgcgcggtgt tgatacaggc gctgtatgcc
     9481 tctttcagac ggctgtcttc ctgtggtgcg gttgattgcg cacaagctac cagccccgat
     9541 gccatcatgg cgatgagaat cagttttttc attgaatgcg gcaggcacaa aacctgccgc
     9601 gtcgggcatc agaagatggt gaatggtgcg attaccataa atttcacgtc acgctcatcc
     9661 tggaagatgt taccgtaacc gccgccccag cttgggatgt cggagtggtt gtcgtattcg
     9721 gtgaagtgca gtttgaacat cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca
     9781 tccaggctgt aggcagactc ttcaatagtc cggtttttgt cgtagtacgc atccggattg
     9841 ctctgccagg tcgcaggttt agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag
     9901 cctggaagat tccagttttt caggtcatac atcgcactga agaagaccgc tttttcgccg
     9961 ttagcgttga agtcggaacg gttatcccac cagatatcca ggcggccgtt ggaggaagcg
    10021 taggttgggg tcatacgttg caggaagtat ccctgctgac tgtcagcctt aatccaggtg
    10081 ccttcgaggc gcaaatccac tacgtcagcc gcccggtaac caaaggtcaa cgcttgcagc
    10141 caggcggtgc cgtcataaag atcgttgacg ctgcgatcgt caactttatc gcgggtacca
    10201 tagaactggt agctggtggt taacgggcta ccggcgatat caaatttgta gctggctttg
    10261 gcaaaatatt gatcgatata cccttccgcc tgaccaaatg ccgcttccag tacgaagtta
    10321 tttttgaagt cgtatttcgc accgatggag tgcagataat caactttggt ggttttatcg
    10381 ttctgataaa actcatccat ttccagatgc cacggcgctt tgtattcgtt ggtccacatg
    10441 taggagaaac tcaaggcacc agcatcgccg taatcaaaat tcgccccggc ttccgccccc
    10501 tgataagtac ccggcataaa gctccagtgc ggcgctaaca gcgtttgacc ggttggctga
    10561 atgtaacctg ccctcgccca aaccggaccg tatttaaatt tggccgcagc tttatacagg
    10621 cttataccgc ttttgtcgcc ggaccagtct tcgtcatagg ctttattact ttttgaaaac
    10681 gcgatttcgt tcgggtggga gctgtcgccg ttttccgcca tttcaatcgc cgtaaacgca
    10741 gcaatatcaa ggccgaacat atcagcagca taaccggact gaaaatcgag gttggcattc
    10801 caggtggagt gagaaaggtt ggttttgtat ttgtcgccgt cggtaacatc tttacggtcg
    10861 cgttcacgct gccagtaata gataccgccg gttaaggttg aatcgtcgat gaatccttcg
    10921 gcgcgagcct ccggcattgc cataaagccc gacattgctg taacaccggc aatagccagc
    10981 gccagcgtac tacgtttgcc actaaacgta cgcatgggtt aatcctcttt gacgtataaa
    11041 ttgctgcacc aaaggtgaac agcgaaaaaa aataaaaatt aaaaaagtag cgttgagaaa
    11101 agtctcgctg actacaggaa cagactattt ttcgcgacgc gaattatcaa tctctttctg
    11161 taaccagaat gtaagaacat gagcaaccgc acatattttg atgatttttg ttacaaggct
    11221 taagtttgcg gttgaaaggc ttgcagaaga ataaaaacgt gaaattccag caaga
//
LOCUS       DAZOYF010000130        11064 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZOYF010000130 DAZOYF010000000
VERSION     DAZOYF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.131"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            198..2426
                     /gene="crfC"
                     /locus_tag="ACHUOF_003152"
     CDS             198..2426
                     /gene="crfC"
                     /locus_tag="ACHUOF_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR0612216.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2423..3301
                     /locus_tag="ACHUOF_003153"
     CDS             2423..3301
                     /locus_tag="ACHUOF_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFR0612217.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            3565..5067
                     /gene="proP"
                     /locus_tag="ACHUOF_003154"
     CDS             3565..5067
                     /gene="proP"
                     /locus_tag="ACHUOF_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR0612218.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5179..5268
                     /gene="pmrR"
                     /locus_tag="ACHUOF_003155"
     CDS             5179..5268
                     /gene="pmrR"
                     /locus_tag="ACHUOF_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR0612219.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5244..6344)
                     /gene="pmrB"
                     /locus_tag="ACHUOF_003156"
     CDS             complement(5244..6344)
                     /gene="pmrB"
                     /locus_tag="ACHUOF_003156"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR0612220.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6345..7013)
                     /gene="basR"
                     /locus_tag="ACHUOF_003157"
     CDS             complement(6345..7013)
                     /gene="basR"
                     /locus_tag="ACHUOF_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR0612221.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(7010..8653)
                     /gene="eptA"
                     /locus_tag="ACHUOF_003158"
     CDS             complement(7010..8653)
                     /gene="eptA"
                     /locus_tag="ACHUOF_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR0612222.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8757..10094)
                     /gene="adiC"
                     /locus_tag="ACHUOF_003159"
     CDS             complement(8757..10094)
                     /gene="adiC"
                     /locus_tag="ACHUOF_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR0612223.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10231..10992)
                     /gene="adiY"
                     /locus_tag="ACHUOF_003160"
     CDS             complement(10231..10992)
                     /gene="adiY"
                     /locus_tag="ACHUOF_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR0612224.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
BASE COUNT         2702 a         2705 c         2874 g         2783 t
ORIGIN      
        1 tgttcttttt ttattcacat gatattttta tctttctgac gttttaaacg catcagaaga
       61 cgttatttat tctgataaaa aaacaacaca ctaaatataa aattatttct tgcagtaatt
      121 ataaaagccg aaaacagaac tcaaaaacaa tctggctaaa taaaagtaac aaaatttgct
      181 ttaaggaaga attttctatg tacacacaga ccctgtatga gttaagtcag gaggctgaac
      241 gcctgttaca gctttctcgc caacagttgc agttactgga aaaaatgcct ctctctgtac
      301 ccggagacga cgcgccacaa ctggctttac cctggagtca gcctaatatc gccgaacgtc
      361 acgcgatgct gaataatgag ttgtgtaaaa tttcccgact ggaaatggtg ctggcaattg
      421 tcggtaccat gaaagcaggg aaatcaacca ccattaatgc cattgttggt acggaggttc
      481 tgcctaatcg taatcgccca atgactgcgc tgccgacgct tattcgccat acgcccgggc
      541 aaaaggaacc ggtactgcat ttttcacatg tcgcgccaat cgattgttta attcaacaat
      601 tacaacagcg cctgcgtgat tgcgatatta agcatctgac cgatgtgctg gaaatagata
      661 aagatatgcg tgcgcttatg cagcggatcg aaaatggcgt cgctttcgaa aaatattatc
      721 tgggtgccca gcctattttt cattgtctga aaagtttgaa tgatttagtg cgactggcga
      781 aggcgctgga cgtcgatttt cctttttctg cttacgccgc cattgagcat attcccgtga
      841 ttgaagtgga gtttgtccat ctggcggggc tggaaagtta tcccggtcag ttgacgttac
      901 tggatacccc cgggccaaat gaagccgggc aaccgcatct gcaaaaaatg cttaaccagc
      961 agctggcacg cgcctcggcg gtactggcgg tgctggatta tacgcaactg aaatcgatct
     1021 ccgatgaaga ggtccgtgag gcgattttgg cggtggggca atcggtgccg ctgtatgtgc
     1081 tggtcaataa gttcgatcaa caggatcgta acagtgacga cgccgaccag gtgcgggcac
     1141 tgatttccgg gacgctgatg aaaggctgta ttacgccaca gcagatattc ccggtgtcgt
     1201 cgatgtgggg ctacctggcg aatcgggcgc gctatgagtt agccaacaac ggtaagttac
     1261 cagcgccaga gcaacaacgc tgggtggaag attttgccca tgccgcgctc ggcaggcgct
     1321 ggcgtcatgc cgatctggcg gacctcgaac atattcgtca tgctgccgat cagttgtggg
     1381 aagattcgct gttcgcccag ccaattcagg cgttgcttca tgccgcttac gctaacgcct
     1441 cattgtatgc tctgcgatct gctgcgcata aactgttgaa ttacgcgcag caggcgcggg
     1501 aatacctgga ttttcgtgcg cacgggttaa acgtcgcttg tgaacaattg cggcaaaata
     1561 tccatcaggt cgaagaaagt ttgcagctat tgcaactcaa tcaggcgcag gtgagcggcg
     1621 agattaaaca tgaaatcgag ctggccctga cctccgccaa ccactttctg cgtcaacagc
     1681 aagatgcggt gaatgcgcag ttagccgcct tgtttcagga tgattcggag ccgttaagcg
     1741 agattcgtac ccgctgtgag acactgttac agacggcgca gaacaccatc agtcgcgact
     1801 ttacgctgcg ttttgccgag atggaatcca ccctttgccg ggtgttaacc gatgttattc
     1861 gccccattga gcaacaagtc aaaatggaat tgagcgagtc agggtttcgt cctgggtttc
     1921 attttcctgt ttttcacggc gtagttcccc acttcaacac tcgccagctg ttcagtgaag
     1981 tcatttcgcg ccaggaagca acggacgagc agagcacgcg tttaggcgtt gtgcgtgaga
     2041 ctttttcgcg ctggttgaat cagcccgact ggggacgggg aaatgagaaa tccccgacag
     2101 aaacggttga ttacagtgtg ttgcaacgag cattaagcgc agaagtcgat ctctattgcc
     2161 aacaaatggc taaagttctg gcagagcagg tcgatgaatc tgttacggca ggcatgaata
     2221 cttttttcgc tgagttcgct tcatgtttga cagaattaca gacgcgttta cgcgaaagtc
     2281 tggctctgcg tcaacaaaat gaatcggtgg tcaggctgat gcagcagcaa ttgcagcaga
     2341 ctgtgatgac tcacagctgg atttacaccg acgctcagct gttacgcgat gatattcaaa
     2401 cacttttcac ggcagaacga tattgaccaa gacgttactg gacggccccg gtcgcgtgct
     2461 ggagtcggtt catccgcgct ttttagtgga tctggcgcag ggcgatgatg cccgccatcc
     2521 tcaagcccat cagcagcagt ttcgtgaacg actgatgcag gaacttcttt cgcgtgtgca
     2581 gcttcagaca tggacgaacg gcggcatgtt aaatgcgccg cttagcctgc gactgacaat
     2641 ggtggaaaaa ctggcgtcga tgctggatcc cggtcatctg gcgctgacgc agatcgcgca
     2701 gcatctggcg ctgctgcaaa aaatggatca ccgccagcac tctgctttcc cggagctccc
     2761 ccagcaaatt gccgccttgt atgagtggtt ttcagcccgt tgtcgctgga aggaaaaggc
     2821 gttaacgcaa cgaggcctac tggtgcaggc aggtgatcag agcgagcaaa tttttacccg
     2881 ctggcgtgct ggggcgtata acgcctggtc gttgcctggg cgctgtttta tcgttctgga
     2941 ggagttgcgc tggggtgcat ttggcgatgc ctgccgtctg ggaagcccgc aagcggtggt
     3001 gttgttgctg ggtgatttgc gcgagaaagc gacacaacat ctggcagaga gtatcaatgc
     3061 ggcaccgact acgcgtcact attaccatca gtggtttgcc tcttcgaccg ttccgacggg
     3121 cggggatcat gctgattttt taagttggct gggaaagtgg accacggcag ataaacaacc
     3181 cgtttgctgg tcagtgaccc aacgctggca aactgtcgcg ctggggatgc cacgactctg
     3241 ttcagcgcag cgtttggtgg gggcaatggt cgaggaaatc ttctctgtaa atttggcgta
     3301 aataatcagt tacatcaatg agtcctaaac gaaatccatg tgtgaagttg atcacaaatt
     3361 taaacactgg tagggtaaaa aggtcattaa ctgcccaatt caggcgtcaa ctggtttgat
     3421 tgtacattcc ttaaccggag ggtgtaagca aacccgctac gcttgttaca gagattgcat
     3481 cctgcaattc ccgctcccct tttgcggccg tcgcgctgat ttttctggcg tttgcggaaa
     3541 tgggccaact ctgcgaggaa agctatgctg aaaaggaaaa aagtaaaacc gattaccctt
     3601 cgtgatgtca ccattattga tgacggtaaa ctgcgtaaag ccattaccgc agcatcactg
     3661 ggtaatgcaa tggaatggtt tgattttggt gtttatggtt ttgttgctta cgcattaggt
     3721 aaagtttttt tcccgggggc tgaccccagc gtgcagatgg ttgctgcact tgccactttc
     3781 tccgttccct ttctgattcg accgcttggc ggactcttct ttggtatgtt gggcgataaa
     3841 tatggtcgcc agaagatcct cgctatcact attgtgatta tgtcgatcag tacgttctgt
     3901 attggcttaa taccgtccta cgacacgatt ggtatttggg caccgattct gctgttgatc
     3961 tgtaagatgg cacaaggttt ctcggtcggt ggtgaatata ccggggcgtc gatatttgtt
     4021 gcggaatact cccctgaccg taaacgtggc tttatgggca gctggctgga cttcggttct
     4081 attgccgggt ttgtgctggg tgcgggcgtg gtggtgttaa tttcgaccat tgtcggcgaa
     4141 gcgaacttcc tcgactgggg ctggcgtatt ccgttcttta ttgctctgcc gttagggatt
     4201 atcgggcttt acctgcgcca tgcgctggaa gaaactccgg cgttccagca gcatgttgat
     4261 aaactggaac agggcgaccg cgaaggtttg caggatggcc cgaaagtctc gtttaaagag
     4321 attgccacta aatactggcg cagcctgttg acatgtattg gtctggtaat tgccaccaac
     4381 gtgacttact acatgttgct gacctatatg ccgagttatt tgtcgcataa cctgcattac
     4441 tccgaagacc acggggtgct gattattatc gccattatga tcggtatgct gtttgtccag
     4501 ccggtgatgg gcttgctgag tgaccgtttt ggccgtcgtc cgtttgtgct acttggtagt
     4561 gttgcactgt ttgtgttggc gatcccggcg tttattctga ttaacagtaa cgtcatcggc
     4621 ctgatttttg ccgggttact gatgctggcg gtgatcctta actgctttat gggcgttatg
     4681 gcttctacct tgccagcgat gttcccgacg catatccgtt acagcgcgct ggcggcggca
     4741 tttaatattt cggtgctggt tgccggtctg acgccaacac tggcggcctg gctggtcgaa
     4801 agctcgcaga atctgatgat gcctgcctat tacctgatgg tagtggcggt gattggttta
     4861 atcaccggcg taaccatgaa agagacggca aatcgtccgt tgaaaggtgc gacaccggcg
     4921 gcgtcagata tacaggaagc gaaggaaatt ctcgtcgagc attacgataa tatcgagcag
     4981 aaaatcgata atattgacca cgagattgcc gatttgcagg cgaaacgtac ccgcctggtg
     5041 cagcaacatc cgcgaattga tgaataagct gaaacggatg gcctgatgtg acgctgtctt
     5101 atcaggccaa ttgaactctt aaggttcact taatctctga cgcgcatact ctcctccagg
     5161 ttaacggagg agagtgcaat gaaaaaccgt gtttatgaaa gtttaactac cgtgttcagc
     5221 gtgctggtgg tcagcagctt tctttatatc tggtttgcca cgttctgatc tttcttcagc
     5281 cgtacccagg cccgcgtgcc ggaagtctct tgccggtttt gcaggaaaaa ctgcccgtga
     5341 tgcaactgtg taatgcggct gacaatactt aaccccagac caatcccgcc ataacggctg
     5401 tccatacgta caaacgcttt actcaactcc ccgcatttac tctcatcaat acctggtcct
     5461 tcatcttcaa ctgccatgac cgctccgcca tcttcttgca gcttaatcat aatgttgctg
     5521 ccttgcgggc tgtaacgatg ggcgttttct accaggtttc gcaataacat ccgcagcagg
     5581 gttgcatcac cctgaacggt gatgtcggcg gcgctctctg gcaatagcag ggtttgctgt
     5641 cgctggtcga gcatggtact gagttcgtca tacgagggga gaatgacatc ttccagcagt
     5701 tttacatgtt gataattacc ggaagaaaat gactgtccgg cacgcgccag ttgcagcagc
     5761 tgggagacgc tctccatcat ctgatcaagc cgtgccacta acggtgctac atcaatgtga
     5821 tgcgttttcg ccagcagttc cagatgcaaa cgcacccccg ccagtggcgt tcgcagttcg
     5881 tgcgcgacgt cagcggtaaa caacctttcg ttatccagcg tgctggtcag gcgactgacc
     5941 agatcgttca acgccgaaac caccgcttcg atttcgaggg tggcgctgtg aatggcaatg
     6001 ggcgttaagt tgtcggcggt gcgcgcttcc agctcttttt gcagctccgc cagcgggcgg
     6061 gtgatgcggc gtaccgcctg atagcagata aatagcgtca ggctgaccat aaagacgccg
     6121 gggacaatca ggctggcgac cgcctcgcgg atctcacgca tgatgtggcg atcgttgttg
     6181 cgattgtcgc gtagcgcctg ctcaaacagc tgaatctgct cggtactttc atgccatagc
     6241 cagaagacgc tgatcagctc aaacaccaac aaaatggccc cgatggtcaa tatcagccgt
     6301 tggcgcagcg atataggtcg gcgcagaaaa cgcatcagat tcaattagtt ttcctcattc
     6361 gcgaccagca tatagccaaa gccgcgcacg gtgcggatac gggctttgcc cactttgtcg
     6421 cgcagattgt ggatatgcac ttccagggtg ttggtcgagg gttcattgtc ccagttatag
     6481 atgtcgttgt agagaatttc ccgatgtacc ggactgcctg ctttgagcat taaccgtgac
     6541 agcagagcat attctttggg cgtcagaatc aactcttcac cgcccatcca tacctgacgg
     6601 cgacccatat tcagcgtcag attgccaaca atcagctcac tttcgccctg attattatgg
     6661 cgtcgtagca gggcgcggat acgggcgtgt aactcttcca gcgcaaaagg cttcaccaga
     6721 tagtcatcgg caccgacatc cagcccggcg attttgtcgg tcagcgtatc gcgagcggtg
     6781 aggatcagta ccggcagggt gtattttttc tgccggatac gggcgagaaa atgcagtcca
     6841 tcttcgtcgg gtaaccctaa atccagtacc accaggctgt aatgaccgtc ctcaaggctt
     6901 tgttccgcca tccgcgcggt tgtcacgcca tcgcacgcgt agccttcggt ttgcgccgcc
     6961 agaatcagtc cctgcaataa cagcgtatcg tcttcaacaa tcagaatttt catttactca
     7021 ctctcctgca agtttgcaga atatcatccg cggcctggta atacttcgtc tcaacgccag
     7081 ttaatcccaa tagcgtggag aataaattgt cttgtgaata gtgttgcgtt tgcgcctgtt
     7141 tttgcaggca gttctggtca acctgatacc gcttttgata atcctccgac agccacagca
     7201 gcattggcac ctgtttttgg ctatccgggg cgatggcata aggcagaccg tgcagataga
     7261 tgccattttc acctaacgat tcaccgtggt cagaaagata aaccaggctg gtggtaaatt
     7321 tatcctgatg ttctttaagc agattaatcg ctttatcaac aatatagtcg acgtaaacca
     7381 gcgtgttgtc gtaagtgttc accagttgct ctttggtaca ggtctggatc tcattggtgt
     7441 cgcaggttgg ggtaaatttc ctgaactgcg gcggatagcg gttgtaatag gtcggaccgt
     7501 ggctgccgat ggtgtgtaag acaatcacgc catcaccctg caggttattg atgtactctt
     7561 ccagcccgtg gaacagcact tcgtcatagc attcgccgtt gatgcactga tcaggcagat
     7621 tcagcgcggt gacgttctgg tgaggcacgc ggtcgcaggc acctttacag ccgccatcgt
     7681 tgtcattcca cagcacgttg atgcccgctc gctgaatgat atccagcacg ccttcctggt
     7741 gctgtgccag ctcttctttg tagtgctcac gcggcatatc cgagaacatg cacggtactg
     7801 aaactgccgt tgccgtgccg caagatgcgg tattagggaa atagaccacg ttatctttcg
     7861 ccagccgcgg gttagtttca cgcgggtagc cgttgaggga gaagttctcc gcccgcgagg
     7921 tttcgccgac aatcaggatg gtcaaatttt tacgtttttc gttctgcatt aacgggttgc
     7981 ggtgcgcgtc ttcaccaatt cgcaccagcg gcagatttgc cagtcgctga tgggagtacc
     8041 atgaccagct ggcaacaatg ctgttagagg ggcttaagga tttcaccagc tctttgttat
     8101 tgcggaacaa cgaggcgtag tctttataaa acagtgcggc gaccagcaaa atcagcagta
     8161 cagaaaccag aatattggct ccacggaaaa gaacactgcg cagacgcgag gtggcaggtt
     8221 tgatttttag ccagcaggca atcagctcag caagcacgcc gctgaatccc agcgttaata
     8281 acatttgcgg tgtcatcagc gcataacttt ctgctggagt ggtatcaata atattggcaa
     8341 tcatcgagcg gtcgatgacg atgccgtaag tcattatgaa atattgtgca gccgcgccaa
     8401 tcagaataaa caggcaggcc agtggtcgat taagccataa gaaagagctt agtgtcagga
     8461 caatattaat cacgctgaaa gcgacaaccg gcatcgacaa gaaaaccagt acgttatgca
     8521 gcgaatccag cggcagcgcc tgcaacacct gtttaaaaaa ggcaatattc aggcagatag
     8581 agatataaaa agcggccaac aatagccagg cgagtaaatt caaagagggt ctttttagta
     8641 ggcgcttcaa catcacggtg tttccatcga acaaagtgcg catatgctcg caaagcaaaa
     8701 ttaagccaac cttaagttct taaggttggc ttttatgttt gctggattaa ggggaattaa
     8761 tctttgctta ttggtgcatc taagggatac gggtttttat gtagccggtt gtaattcagc
     8821 gcatacatgg cggtgatgac catcagggtg acaaatgacc acataacctc tttcgccccg
     8881 gaccccacca cggcccaaat gcagtagagg aaggcaatgg tagtaacagc cagatatgcc
     8941 gggcgtgctt taccaaagtg accgtggccg agcagcagta acgccgcaca ggtgtaaaga
     9001 tatggcacca gtgtaaagat gaccgagacg gaagaaacca gaccgaactc tttggtcgcg
     9061 tttggtgaaa tgctgctgag ctggaagatg gtcatcaaaa taccgacgat aatcagcccc
     9121 gccactggcg tacccgcttt atttacacgg gcaaaaatcg gtgggaacag tccgtcatcg
     9181 gcagcggctt tcgccgtttg acccgccagc aacgtccagc cgcccagtga acctaagcaa
     9241 cccgcagctg cgcagaagga aacaatggcc ccagcggtgt cacccaacgc catccgtgcg
     9301 gcatcaccga atggcgaggc agaaacgcgc agtgcggcat tagggatcat ccccataatc
     9361 gcggtggtgg agagcacata gcaaacggcg gcaatcaata ccccaccaat ggtggcgata
     9421 gggacattgc gtttcgggtt tttcaccaca cctgcggcaa cggaggcact ttccacaccg
     9481 atgaacgacc acagcgtaac gttaagggta ctttgaatcg caccgaaggt gcccaggccg
     9541 ctgacgttcc atgccgccat ataggtttca ccacggaacc agaaccagcc aaatacggca
     9601 atcccgacga tggggatcag cgccagcaca gtggcaacgg cctgcacgcg ggtgatcatt
     9661 ttcggaccga caatgttcag caggacgaag atccacagca ccacgacgca ggtgatggtt
     9721 aataccaatg gatctttcag aatcgggaag aagtaactta aatatcctac gccaatgacc
     9781 accatggcga tattgccgat ccagcaggcc agccagtaga ggacgttggt ttgataaccg
     9841 agaaacgggc caaagcagcg gcgggcgtaa gcgtaagaac caccaggact tgggtcgagg
     9901 aacgacattt tggcgtatac catcgagagc cccagcgcac cgataatcgt caccaaccat
     9961 ccataaatgg caatcccgcc agtggaggcc aggtttgcag gtaacagaaa aacacctgac
    10021 cccataatat tccccgacac catcagggtg acggggatta agcccacttt gtgagcatca
    10081 gcatccgaag acataattaa actcctgcga aggcgagctt cgtgacaata aattacgtca
    10141 tatcatacgc ctgcattcga tagttgaatt attatcgacc gggttatcgc tgatgccgtt
    10201 attaatatag ctcttcgtta aggttaaaaa tcaggcgaca gtgcgttctc tgtgctgact
    10261 aacataatgc agcggcgtca taccgtagaa gtctttaaag acagaaataa agtacgacgt
    10321 actgttgtag ccgcaggact gtgatacctg agagatattt ttaccgtcca tcattaattc
    10381 atttacggca taacgcatgc ggcaggtggt gattatttgg ctataactgg tgttttcgct
    10441 tttcaacttt ttctttaaca gacttgggct aagacataaa caactggcta ccatactaag
    10501 attccagtct ttgtgaatat cgctttcaat aatttgataa acgctgtcac ttacacaatt
    10561 acgtaacata tacatcatta atgaaaggaa ttttttactg tcgagaaaac gggatagcac
    10621 cgtgaataat aatgcgcgtg ttcgttcctt ttctgactca gtttctgcgg gcatcatgct
    10681 gtgttgcgcg gcaacgcgaa agacgtctgg cgtcaggcat ggcagggtga gtatgggcgt
    10741 agcgctacgt tgccatactg gtatttgcga gagatcttta ttcagaaacc ggaggtaatc
    10801 tttgatgatg tcgtgactaa tatgagcaac caaagtgttg ttaagagagg agacgtcgat
    10861 aatattattt tcacaattta ataacgccat atggttagct ttaaggctaa taggttcttt
    10921 cccattcacc cttatccaga catctttttc agtcaataaa acaatacaag gttggtcgct
    10981 gcaaatcctc atacatgtac tcctgagtgc gaataaaaga ccagttctcg ctaaagcaaa
    11041 gcgataccgg gaaatggtta tata
//
LOCUS       DAZOYF010000131        10927 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZOYF010000131 DAZOYF010000000
VERSION     DAZOYF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.132"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            157..504
                     /locus_tag="ACHUOF_003161"
                     /pseudo
     CDS             157..504
                     /locus_tag="ACHUOF_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            557..952
                     /locus_tag="ACHUOF_003162"
     CDS             557..952
                     /locus_tag="ACHUOF_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR0612225.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="ACHUOF_003163"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="ACHUOF_003163"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR0612226.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="ACHUOF_003164"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="ACHUOF_003164"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR0612227.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACHUOF_003165"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACHUOF_003165"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR0612228.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="ACHUOF_003166"
     CDS             complement(5323..6423)
                     /locus_tag="ACHUOF_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR0612229.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="ACHUOF_003167"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="ACHUOF_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR0612230.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="ACHUOF_003168"
     CDS             complement(7571..8137)
                     /locus_tag="ACHUOF_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0612231.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="ACHUOF_003169"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="ACHUOF_003169"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR0612232.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(10128..10172)
                     /locus_tag="ACHUOF_003170"
     CDS             complement(10128..10172)
                     /locus_tag="ACHUOF_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612233.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            10263..10751
                     /locus_tag="ACHUOF_003171"
     CDS             10263..10751
                     /locus_tag="ACHUOF_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR0612234.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2669 a         2789 c         2605 g         2864 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgcc
//
LOCUS       DAZOYF010000132        10862 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZOYF010000132 DAZOYF010000000
VERSION     DAZOYF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.133"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            109..1008
                     /gene="lysO"
                     /locus_tag="ACHUOF_003172"
     CDS             109..1008
                     /gene="lysO"
                     /locus_tag="ACHUOF_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR0612235.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1152..2804
                     /gene="hcp"
                     /locus_tag="ACHUOF_003173"
                     /gene_synonym="priS"
     CDS             1152..2804
                     /gene="hcp"
                     /locus_tag="ACHUOF_003173"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR0612236.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            2816..3784
                     /gene="hcr"
                     /locus_tag="ACHUOF_003174"
     CDS             2816..3784
                     /gene="hcr"
                     /locus_tag="ACHUOF_003174"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR0612237.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3917..5635
                     /gene="poxB"
                     /locus_tag="ACHUOF_003175"
     CDS             3917..5635
                     /gene="poxB"
                     /locus_tag="ACHUOF_003175"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR0612238.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5672..6673
                     /gene="ltaE"
                     /locus_tag="ACHUOF_003176"
     CDS             5672..6673
                     /gene="ltaE"
                     /locus_tag="ACHUOF_003176"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR0612239.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6684..8114
                     /locus_tag="ACHUOF_003177"
     CDS             6684..8114
                     /locus_tag="ACHUOF_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR0612240.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8213..9226
                     /locus_tag="ACHUOF_003178"
     CDS             8213..9226
                     /locus_tag="ACHUOF_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR0612241.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9223..10053)
                     /gene="amiD"
                     /locus_tag="ACHUOF_003179"
     CDS             complement(9223..10053)
                     /gene="amiD"
                     /locus_tag="ACHUOF_003179"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR0612242.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10050..10373)
                     /locus_tag="ACHUOF_003180"
     CDS             complement(10050..10373)
                     /locus_tag="ACHUOF_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR0612243.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10584..>10862)
                     /locus_tag="ACHUOF_003181"
     CDS             complement(10584..>10862)
                     /locus_tag="ACHUOF_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0612244.1"
                     /translation="NNVKVAADAFLEIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAY
                     INDKSIEILLLSCFAAADQKAIQRYRPQSQDGEDFRSHLQYKLPLSAH"
BASE COUNT         2476 a         2881 c         2872 g         2633 t
ORIGIN      
        1 ccctctgatg cataacattt ccttttaatt gcaactttac tcgcccttcc gctttcagta
       61 gtgtgtcatc gcgcatttcg acttcttctt tgcaaggact ggattttcat gttttctggg
      121 ctgttaatca ttctggttcc cctgattgtg ggttacctca ttccgcttcg ccaacaagct
      181 gcgttaaaag ctattaatca gctattaagc tggatggttt accttattct cttttttatg
      241 ggtatcagtc tggcgtttct cgataacctc gccagtaacc tgttggcgat tctgcattat
      301 tctgccgtca gtattaccgt tattttactg tgtaatattg ccgccctgat gtggctggag
      361 cgaggcctgc cgtggcgcaa ccaccatcag caagaaaaac tcccgtcgcg tattgcgatg
      421 gcgctggagt cgctaaaact gtgcggcgta gtagtgattg gttttgccat tggtctgagt
      481 ggactggctt tcttacaaca cgcgaccgaa gccagtgaat acacgttaat tttgctactt
      541 ttcctcgttg gtattcagtt gcgcaataat ggcatgacct taaagcagat tgtccttaat
      601 cgccggggaa tgattgtcgc cgtggtggtg gttgtcagtt cattaattgg tggtttaatt
      661 aacgccttta ttcttgatct ccccatcaat accgcgctgg caatggcctc cggtttcggc
      721 tggtattctc tttccggtat tttattgacc gaatcttttg gtccggtaat cgggagcgcg
      781 gcgtttttta atgatctggc ccgtgaactg attgctatta tgttgatccc tgggctgatt
      841 cgccgcagcc gctctactgc actgggctta tgcggtgcca catcaatgga tttcaccctg
      901 cccgttcttc aacgtactgg cgggctggat atggtcccgg cggcaattgt tcacggtttt
      961 attcttagcc tgttagtgcc gatcctcatc gcctttttct ctgcgtaata cctctctggc
     1021 ggtagttccc tgccgccaaa attgcgctaa atcaatctcc cttaaagttg catgaaaaat
     1081 cccttttatc cccgcgttaa gcgtcttacc cttaaacatg tatattaaat ataactttaa
     1141 aaggtgtgat catgttttgt gtgcaatgtg aacaaactat ccgtactccg gcagaaaacg
     1201 gctgctcata cgcgcagggg atgtgtggta aaacggcgga aacttctgac cttcaggatt
     1261 tactcatcgc ggcgctgcaa gggctttctg cctgggcggt aaaagcgcgt gaatacggca
     1321 tcatcaacca cgatgttgac agcttcgcgc cacgtgcctt tttctcaacc ctgaccaacg
     1381 ttaacttcga ttctccgcgt attgtcggct acgctcgtga agcgattgcc ttgcgcgagg
     1441 cgctgaaagc acaatgcctg gctgtagatg ccaacgcgcg cgtcgataac ccgatggctg
     1501 acctgcaact ggtgagcgat gatctcggcg agctgcaacg tcaggcagca gaatttaccc
     1561 ctaacaaaga taaagcggcg attggcgaaa acattctcgg cctgcgtctg ctgtgcctgt
     1621 atggcctgaa aggtgcggcg gcctatatgg aacacgcgca tgttctcggt caatacgaca
     1681 acgatattta tgcccagtac cataaaatca tggcgtggct ggggacctgg cctgccgata
     1741 tgaacgcgct tcttgagtgt tcaatggaaa tcggccagat gaacttcaaa gtgatgagca
     1801 ttctggatgc aggcgaaacc ggtaaatacg gtcacccgac gccaacccag gtcaacgtca
     1861 aagcgacggc gggtaaatgc attctgattt ccggtcacga tctcaaagat ctctacaacc
     1921 tgctggaaca gaccgaaggc acgggcgtta atgtctacac ccacggcgaa atgctgcctg
     1981 cgcatggcta cccggagctg cgtaaattca agcatctggt cggtaactac ggcagcggct
     2041 ggcagaatca gcaagtggag ttcgctcgtt tccctggccc catcgtgatg acctcgaact
     2101 gcatcatcga cccaaccgta ggcgcttatg acgatcgtat ctggacccgc agcatcgttg
     2161 gctggcctgg cgtgcgtcat ctggatggcg aagatttctc tgcggtcatc gcccaggcgc
     2221 aacaaatggc gggcttcccg tacagcgaaa ttcctcacct gatcaccgtg ggctttggtc
     2281 gccagacgct gcttggtgca gccgatacgc tgattgatct ggttagccgt gaaaaactgc
     2341 gtcatatctt cctgcttggt ggctgtgacg gcgcacgcgg cgagcgccac tacttcaccg
     2401 atttcgccac cagcgtgccg gatgactgcc tgatcctgac cctcgcctgt ggtaaatatc
     2461 gctttaacaa acttgagttt ggcgatatcg aaggtctgcc gcgtctggta gatgcaggtc
     2521 aatgtaacga tgcttactca gcaattattc tggctgtcac tctggcagag aaactgggct
     2581 gtggtgtgaa cgatctgccg ctgtcgctgg tgctctcctg gtttgaacag aaagcgattg
     2641 tcattctgct gacgctgctt tctctgggcg tgaaaaatat cgtcaccggc ccgactgcac
     2701 ctggtttcct gacaccagac ctgctggcgg tgctgaacga gaaattcggc ctgcgttcta
     2761 tcaccactgt tgaagaagac atgaagcaac tgttgaacgc gtaaggagtt tgtcgatgac
     2821 gatgccaacg aatcaatgcc cgtggcggat acaggttcat cacattacgc aagaaacgcc
     2881 ggatgtgtgg acgatttccc tgatttgcca cgatcactac ccatatcgcg ccgggcaata
     2941 tgcactggtc agcgtgcgaa actcagcgga aacgctgcgt gcttacacca tttcctccac
     3001 gccaggcgtg agtgaatata tcaccctgac cgtgcggcgg attgatgacg gtgtcggctc
     3061 ccagtggctg acgcgcgatg taaaacgcgg tgattatctc tggctttcgg acgcgatggg
     3121 ggaatttacc tgcgacgata aagcagaaga taaattcctg ttgctggcgg caggctgcgg
     3181 cgtcacgccg attatgtcga tgcgtcgctg gcttgcgaag aaccgtccac aggccgatgt
     3241 gcgggtgatc tacaacgtgc gtacgccgca ggatgttatt ttcgccgatg agtggcgtaa
     3301 ctatccggta acgctggtgg cagaaaataa cgttaccgaa ggctttatcg ctggtcgcct
     3361 cactcgcgaa ctgctggcag atgtacctga cttagcttca cgtaccgtga tgacctgcgg
     3421 cccggctccg tatatggatt gggtagagca ggaagtgaaa gcgctcggcg tgacgcgttt
     3481 ctttaaagag aaattcttca ccccagtagc agaagcggcg accagcggtc tgaaattcac
     3541 caaactgcaa ccggcacgag aattttacgc cccggttggc accacgctac tggaggcgct
     3601 ggaaagcaat aacgttccgg ttgtcgctgc ctgccgtgcg ggtgtttgcg gctgctgtaa
     3661 gacaaaagtg gtttccggtg aatatacggt gagcagtaca atgacgctga ccgacgccga
     3721 aatcgctgaa ggttacgtac tggcctgctc ctgccatccg cagggggatt tggttctcgc
     3781 ataatcgcct tatgcccgat gatattcctt tcatcgggct atttaaccgt tagtgcctcc
     3841 tttctctccc atcccttccc cctccgtcag atgaactaaa cttgttaccg ttatcacatt
     3901 caggagatgg agaaccatga aacaaacggt tgcagcttat atcgccaaaa cactcgaatc
     3961 ggcaggcgtg aaacgcatct ggggagtcac gggcgactct ctgaacggtc ttagcgacag
     4021 tcttaatcgc atgggcacca tcgagtggat gtccacccgc catgaagaag tggcggcgtt
     4081 tgccgctggc gctgaagcac aactttcagg agaactggcg gtctgtgccg gatcgtgcgg
     4141 cccaggcaac ctgcacttga tcaacggcct attcgattgc caccgcaata acgttccggt
     4201 actggcgatt gccgctcata ttccctccag cgaaattggc agcggctatt tccaggaaac
     4261 ccacccacaa gagctattcc gcgaatgtag tcactattgc gagctggttt ccagcccgga
     4321 gcagatccca caagtgctgg caattgctat gcgcaaagcg gtgcttaacc gtggcgtttc
     4381 cgttgttgta ttaccgggcg acgtggcgtt aaaacctgcg ccagaagggg caaccacgca
     4441 ctggtatcat gcgccacagc cagtcgtgat gccggaagaa gaagagttac gcaaactggc
     4501 gcaactgctg cgttattcca gcaatatcgc cctgatgtgt ggcagcggct gcgcgggggc
     4561 gcataaagag ttagttgagt ttgccgggaa aattaaagca cctattgttc atgccctacg
     4621 cggtaaagag catgtcgaat acgataatcc gtatgatgtt ggaatgaccg ggttaatcgg
     4681 attctcgtca ggtttccata ccatgatgaa cgccgacacg ttagtgctac tcggtactca
     4741 gttcccctac cgtgcgttct acccgaccga tgccaaaatt attcagattg atattaatcc
     4801 agccagcatc ggcgcgcata gcaaggtaga tatggcgctg gtcggcgata tcaaatcaac
     4861 cctgcgcgca ttgctgccac tggtggaaga aaaaaccgat cgcaagtttc tggataaagc
     4921 gctggaagat taccgcgatg cccgcaaagg gctggacgat ttagctaaac cgagcgagaa
     4981 agccattcac ccgcaatatc tggcgcagca aattagtcat tttgccgccg atgacgccat
     5041 ctttacctgt gacgtcggca cgccaacggt atgggcggca cgttatctga aaatgaacgg
     5101 caagcgtcgc ctgttaggtt cgtttaacca cggttcgatg gctaacgcca tgccgcaggc
     5161 gttgggtgcg caggcaaccg agccggaacg tcaggtggtc gccatgtgcg gcgatggcgg
     5221 ttttagcatg ttgatgggcg atttcctctc agtagtgcag atgaaactgc cggtgaaaat
     5281 tatcgtcttt aataacagcg tgctgggctt tgtggcgatg gagatgaagg ccggaggtta
     5341 cctgacagac ggtactgagc tgcacgacac caactttgcc cgcattgccg aagcgtgcgg
     5401 cattacgggt atccgtgtag aaaaagcctc tgaagttgat gaagccctgc aacgcgcctt
     5461 ctctatcgac ggtccggtgt tggtggatgt ggtggtcgcc aaagaagaat tagccattcc
     5521 accgcagatc aaactcgaac aggccaaagg ttttagcctg tatatgttgc gcgcaattat
     5581 cagcggacgc ggcgatgaag tgatcgaact ggcgaaaacg aactggctaa ggtaaaaagg
     5641 gtggcatttc ccgtcataat aaggacatgc catgattgat ttacgcagtg ataccgttac
     5701 ccgaccgagc cgcgccatgc tcgaagcgat gatggccgcc ccggttgggg acgacgttta
     5761 cggagacgac cctaccgtta atgctctgca ggactatgca gcagagcttt ccggtaaaga
     5821 agccgccatt tttctgccta ccggcactca ggccaacctg gtcgctctgc tcagtcactg
     5881 cgaacgtggc gaagagtata ttgtcggtca ggccgcgcat aactatctgt ttgaagccgg
     5941 tggcgcagcg gtgctgggca gtattcagcc gcaacccatc gacgcggctg ccgacggcac
     6001 gctaccgctg gataaagtgg cgatgaaaat caaacccgac gatatccatt tcgcccgcac
     6061 caaattactc agtctggaaa acacccacaa cggcaaagtg ctgccgcgtg aatacctgaa
     6121 agaagcatgg gaatttaccc gcgagcgcaa tctggcgctg catgttgacg gtgcgcgcat
     6181 ctttaatgcc gtggtggctt acggctgcga actgaaagag atcacgcaat attgtgattc
     6241 gttcaccatt tgcctgtcga aaggtcttgg gacgccagtc ggttcattac tcgtcggtaa
     6301 tcgtgattac attaaacgtg ccatccgctg gcggaaaatg gccggtggcg ggatgcgtca
     6361 gtccgggatt ctggctgccg ccgggatgta tgccctggaa aataacgtcg cacggttgca
     6421 ggaagatcac gacaacgccg cctggatggc ggagcaactg cgtgaagcag gcgcggatgt
     6481 gatgcgtcag gacactaata tgctgtttgt tcgcgtcggg gaagaaaatg ctgccgcgtt
     6541 aggcgaatac atgaaagcga gaaacgtgct gattaacgcc tcgccgattg tccgcctggt
     6601 gacgcatctt gacgtctcgc gcgaacaact ggcggaagtc gccgcccact ggcgcgcatt
     6661 cctggcgcgt taaggagaga gacgtgccgc aacgcatttt agttctcggt gccagtggct
     6721 acattggtca gcatctggtg cgcacactca gccagcaagg gcatcagatc ctagcggcgg
     6781 cacgtcatgt cgacaggctt gcaaagctgc aactggcaaa cgtcagttgc cataaagtcg
     6841 atctcagctg gccggataac cttccggccc tgttgcagga tatcgatacc gtctattttc
     6901 tggtgcacag catgggcgaa ggcggcgatt ttatcgctca ggagcgccag ctggctctca
     6961 acgtccgcga tgcgctacgt gaagtaccag ttaagcaatt aatctttctc agttcgttgc
     7021 aggccccgcc acatgagcag tcggatcatc tgcgagctcg tcaggctacg gcggacattc
     7081 ttcgtgaagc gaatgtacca gtgaccgaac ttcgggccgg aattatcgtt ggcgcaggtt
     7141 cagcggcgtt cgaagtcatg cgcgatatgg tctacaacct gccagtgtta acgccgccac
     7201 gctgggtacg ttcacgcacc acgcccatcg cgctggaaaa cttgctgcac tatctggtgg
     7261 cgctgttaga ccatccagcc aacgaacacc gcatcttcga agccgccgga ccagaggtgc
     7321 tcagttatca gcaacagttt gaacatttta tggcggtgag cggtaagcgc cgctggttga
     7381 tccccatccc cctccccacc cgctggattt cggtgtggtt tctcaatgtg attacttccg
     7441 taccgcccac caccgccagg gcgttgattc aggggctgaa acacgatctg ctggcagatg
     7501 ataccgcgct acgtgcactc atcccacaac ggctgatcgc tttcgatgac gcggtacgta
     7561 gcacgttgaa agaggaagaa aaactggtca actccagcga ctggggctac gacgctcagg
     7621 cctttgcccg ctggcgaccg gagtacggtt atttcgccaa acaggcgggg tttaccgtta
     7681 aaacgtccgc cagccttgcg gctttatggc tggtggtgaa ccaaatcggc ggtaaagagc
     7741 gttatttctt tggcaatatt ttgtggcaga cacgggcgtt gatggaccgc gcgatcggtc
     7801 ataatctggc gaaaggccgc ccggagcgcg aatatttaca gaccggcgat gcggtggata
     7861 gctggaaagt gattgtcgtt gaaccgaaaa agcaacttac gttgttattt ggcatgaaag
     7921 cgccggggct gggacgactg tgttttaccc tggaagataa aggtgactat cgtactatcg
     7981 atgtccgcgc tttctggcat ccgcacggta tgccggggct gttttactgg ttattgatga
     8041 tccccgcgca tctgtttatt tttcgcggaa tggcaaaaca aatcgccaga ctggcagaac
     8101 aaagcacaga ttaatcgaca aaacctgcgt tattctttca tgtttcccat tgcatcatgg
     8161 gggaaatcac ggaagaatgc gcacgtcatt ttctcaacaa cagtggattg atatgaaggt
     8221 actggttacc ggcgccacca gcggcttagg tcgaaacgcg gtagagtttt tatgccagaa
     8281 aggcatcagc gtgcgtgcga gcggtcgcaa cgaagcaatg ggtaaattgc tggaaaaaat
     8341 gggcgcagag tttgttccga cggatctgac cgagctggtt tcatcacaag ctaaagtgat
     8401 gctcgcgggc attgatacgc tgtggcactg ctccagcttt acctcaccct gggggacaca
     8461 acaggctttc gatctggcta acgttcgcgc cactcgccgc ctgggtgaat gggctgtcgc
     8521 ctggggtgtg cgtaacttta ttcatatctc ttccccctcc ctgtacttcg attatcacca
     8581 ccatcgcaat attaaagaag attttcgccc tcaccgcttc gccaacgagt ttgcccgcag
     8641 caaagcagcc agcgaagaag tgatcaatat gctttcgcag gcgaatccac aaacgcgctt
     8701 tactattctg cgcccacaaa gtctgttcgg accgcacgat aaagtcttta ttccccgtct
     8761 ggcgcatatg atgcaccact acggcagtat tctgttaccg catggcggca gtgcgctggt
     8821 cgatatgacc tactatgaaa atgccgtgca cgcaatgtgg ctggcaagcc aggaagcctg
     8881 cgataagcta ccttccgggc gtgtgtacaa catcaccaac ggcgagcatc gcacactgcg
     8941 cagcatcgtg cagaagctga tcgacgagtt gaatattgac tgtcgtattc gttccgtccc
     9001 ttacccgatg ctggatatga tcgcccgcag catggagcgt ttaggccgca agtcagcaaa
     9061 agagccgccg ctgacccact acggcgcctc caagttgaat tttgacttta cgctggatat
     9121 tacgcgtgcg caggaagagt taggttatca gccggtcatc accctggatg aaggcatcga
     9181 gaaaactgcc gcctggctac gcgaccacgg aaaactgccg cgctaatcct gcccgtattt
     9241 ctccagcaat gcttcggcaa tcgcctgagt ttctgcatcc gcttcgccgt tatataacgt
     9301 cgggcggaaa tgcatctgga atgccataat cacgcgccgc tgctcgcgcg gtgtcatatc
     9361 aggttttacg tcataaccgt agcgcgccaa aagctccagc aatgacgcgg tatctaccgg
     9421 tgtgtgcggc gcgcgcccgg caaggtaaaa gttcacccgc tgcgcatccg gccaggcacc
     9481 aatcccctgc tgcgccagtt gctgccaggg aaataatggc cccggatcgt ctttgcgctg
     9541 cggtgcgata tccgcatgtg ccactacgtt ttccggcttg atgtgataac gggcaataat
     9601 atctttcgcc agcggaataa gcgcctgaat ctgtgcctgt tcaaacgggg caaaatattt
     9661 cactccggat gatttttgcc agccacggtt ttccagctca atgccaatag aggtgtcgtt
     9721 aaggcgcgtt gccccgcgcc aggcgctaat ccccgcatgc caggccagtt cttgttccgg
     9781 caccagttgc cagatgcgcg gtttaccgtt gtatcgcggt ggcaccgcag ggatcagata
     9841 atgcgagctg acctgcttat cagtcagtgt cgccagcgag ctatcaaaat catctgcggt
     9901 gtagtggatc accagcactt taatgcgcgg atacgccgcc tgcgcctggt gtcgggtatc
     9961 aagctgatat ccctctttct cgacaatgcc tttttcgcct gcacaccctg ccagtaacag
    10021 agcggcagcg accagccaga agactcttct catcgacgcg ttttcaccgc cgtaccgctg
    10081 acgctaacca tcagcatact gccgttttgc ccgaccgttt cgtagtcgat atcaatacca
    10141 actacggcat cggcccccag cgcccgcgcc tgggagccta attcctcaaa ggcgatctcc
    10201 cgtgctttac gcagttcttt ttcatacgca ccggaacgtc cgccaacgat atcgcggata
    10261 ccggcaaaga aatcacggaa aatattggca cctaaaatcg cttcgccggt caccacaccg
    10321 caatattcaa cgatggtcag gccttccaga gttggggttg tagaaaattg catgatcgat
    10381 ctccttgttt gttaagcctt cgatctctaa agcattatcg gactgatatg ccatgttggg
    10441 aagaaggttt tgatcagttt aggaaatcat ctcattgtgt caaaaacacc cacttaagtc
    10501 tttacgatta tccgttgcat aacccgatat tggtttcatt atagggtacc gcttacctgt
    10561 cttattatgt tgcaccaaca acattaatga gcggaaagag gcaacttata ttgtaaatga
    10621 ctcctaaagt cttccccatc ttgtgactga ggtctatacc gctggattgc tttttggtca
    10681 gccgccgcaa aacaagaaag taataagatc tcaatgcttt tgtcattaat ataagcgaag
    10741 atcattcttg caaactcgta tataaacgcc cgatcctgag cgcttaatag tttaatacaa
    10801 tatttatttt taagagagaa aataatttca aggaaagcat ccgcagcaac cttcacgtta
    10861 tt
//
LOCUS       DAZOYF010000133        10858 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZOYF010000133 DAZOYF010000000
VERSION     DAZOYF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.134"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            220..1050
                     /locus_tag="ACHUOF_003182"
     CDS             220..1050
                     /locus_tag="ACHUOF_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR0612245.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1191..1964)
                     /gene="uxuR"
                     /locus_tag="ACHUOF_003183"
     CDS             complement(1191..1964)
                     /gene="uxuR"
                     /locus_tag="ACHUOF_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR0612246.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2179..3639)
                     /locus_tag="ACHUOF_003184"
     CDS             complement(2179..3639)
                     /locus_tag="ACHUOF_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR0612247.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3720..4904)
                     /gene="uxuA"
                     /locus_tag="ACHUOF_003185"
     CDS             complement(3720..4904)
                     /gene="uxuA"
                     /locus_tag="ACHUOF_003185"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR0612248.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5244..6587
                     /gene="gntP"
                     /locus_tag="ACHUOF_003186"
     CDS             5244..6587
                     /gene="gntP"
                     /locus_tag="ACHUOF_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR0612249.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6838..7740)
                     /gene="fimH"
                     /locus_tag="ACHUOF_003187"
     CDS             complement(6838..7740)
                     /gene="fimH"
                     /locus_tag="ACHUOF_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR0612250.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7760..8203)
                     /locus_tag="ACHUOF_003188"
     CDS             complement(7760..8203)
                     /locus_tag="ACHUOF_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0612251.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8276..8806)
                     /gene="fimF"
                     /locus_tag="ACHUOF_003189"
     CDS             complement(8276..8806)
                     /gene="fimF"
                     /locus_tag="ACHUOF_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR0612252.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8816..>10858)
                     /gene="fimD"
                     /locus_tag="ACHUOF_003190"
     CDS             complement(8816..>10858)
                     /gene="fimD"
                     /locus_tag="ACHUOF_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR0612253.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2610 a         2797 c         2661 g         2790 t
ORIGIN      
        1 tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa tgagcgtttt
       61 ttgatacttt agagtgtttc ctacccttta atatcactgt gtagagttga tatcactaca
      121 acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat ttatctattt
      181 ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc attaagcaat
      241 gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa agtcgacagt
      301 gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta tcttctgcct
      361 cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt
      421 aatattggca aacagtcctg gccatttaat tatgtgacag atacatgcag agaattcaat
      481 ggtattacaa caacaggacg tatgctctat cgaaacttga aaatcacctc agctttagac
      541 gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc agaaggttta
      601 cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa
      661 ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat aggatatatt
      721 tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc
      781 tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt cgcccttgcc
      841 gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat agaagcctat
      901 cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg tgatgaagat
      961 gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa
     1021 aatggtgaat ggaattatat tggtaaatag ccactgagaa gaatgagtac taatattcag
     1081 gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat tgcgacttct
     1141 taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg
     1201 actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc gtcaacgttc
     1261 gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc
     1321 gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct
     1381 ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg attgttttcc
     1441 cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt tgcttcagca
     1501 atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct ggaagccagt
     1561 tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc
     1621 agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa
     1681 ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta
     1741 tcaagcacat agataccggc accccggcgc acttccacca gccctttgat ctccagcatg
     1801 atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc
     1861 ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgtg gatcatcgcc
     1921 ccgacttcct ggtaaggtct ttgcgcagag gtgacagatt tcatggtgtt gtgtccgtta
     1981 ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg
     2041 gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag ttggcacgta
     2101 caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga ccagtaagtc
     2161 tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag
     2221 ctgctgatat gccgacgtca ccgcgccaac aaagtcggca ttctgcggca gatcatcggc
     2281 aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt
     2341 aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcattac cctgctcatc
     2401 cacgccctgg gtgtaaccca tccagccagc cacgcccagc gccaggtgac gccagctgcc
     2461 gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact tctggctgcc
     2521 gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag
     2581 cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc
     2641 ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat
     2701 ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat
     2761 acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgtgc ccactttatc
     2821 ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca
     2881 ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg
     2941 cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat
     3001 ccatgccgct agctgcgggt cacgcgcctg agccagcccc agtaccgcga ccttcgccac
     3061 atggccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca gccctttttc
     3121 acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg cagtcgggtt
     3181 ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg ccgcatcagc
     3241 gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc
     3301 gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca tcgaaccgat
     3361 aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca gttgctgttt
     3421 tttcaggttt tcgatcagca tgcggtcgtt gcctggcatc aggttaactt cgcagatgcc
     3481 ccagtcgctg tcggtgcttt ccagcagatg atgggtatac agcgcctggt gcgcgcggtg
     3541 aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg
     3601 gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct
     3661 tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc cggtgggtat
     3721 taacggataa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc ggccaggcct
     3781 ttcagacgac caattgcgga gtaaccaggg ttggttttct tcttcaggtc gtccagcatc
     3841 tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg
     3901 tgttcttctt caacaatcgc tttcaccact ttgtacatat caacgtcacc gttcaggtgc
     3961 gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag
     4021 taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc acgcacgccg
     4081 taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac
     4141 tgcatatctt caatggtgga aacaatgcgc ggcaagccga ggatcgggcg cggcggatcg
     4201 tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat cgctttcagg
     4261 aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag ctccaggtgc
     4321 ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc aatgatgtta
     4381 cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc tgcctgagca
     4441 atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg catttcgaat
     4501 gcagcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg
     4561 aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt gcgaatgcca
     4621 cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgctc atagttgcca
     4681 gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca caccaggcct
     4741 gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccgttc
     4801 gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa
     4861 gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca
     4921 catagtgggt tccacagcaa agagacgcgg aaccttggag ttgtttaggt atccggatga
     4981 acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt
     5041 ggtaagacat gtcaaccaga attctgaaat tggttaaccg cgtcgcaaga atttcactct
     5101 catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc agaggatgtg
     5161 acattcatcg caacaatggt tgaccaattt acataacatg tcgcccaaaa taacaacggt
     5221 tcaaccaagt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg gtattcggca
     5281 ttggcctgat gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg
     5341 tggcggcgct gtccgtcggg atgctggcag gcatggattt gatgtcgctg ctgcacacca
     5401 tgaaagcggg cttcggcaac acgctgggag aactggctat catcgtggtg ttcggtgcgg
     5461 tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat acgctgctgg
     5521 cgcgtctcgg tctgcgctat gtacagctgt cggtgattat catcggcctt attttcggtc
     5581 tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt gttattgccg
     5641 ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg
     5701 cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct tatggcgcgg
     5761 atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta atctgcgcag
     5821 gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag
     5881 cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt ctggtgccgc
     5941 tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg gtaaaagata
     6001 cccctgcctg ggaagtggtt aactttattg gttcctcgcc gattgcgatg tttattgcga
     6061 tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag tgggtgatga
     6121 acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc ggtgcgggtg
     6181 gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc atgttgatgt
     6241 cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta attcgtctgg
     6301 cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt gctgcaatcc
     6361 ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggta ctggcgacgg
     6421 ctgcgggttc caacaccctc acccacatta atgatgcatc tttctggttg ttcaaaggtt
     6481 actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag ctggtcaact
     6541 ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag aacaccagag
     6601 cctgccaatg gcaggctcag actgatgact aaagtctaaa aatgtgcccg gacagtcccc
     6661 tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagtaaagtg cgctttgttt
     6721 atgccagatg cgacgctgac gcgtcttatc tggcctacaa agggctaacg tgcaggtttt
     6781 gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgcgctgt aattttttta
     6841 ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca cctgccctcc
     6901 ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta ctgctcctaa
     6961 cgataccgtg ttattcgctg gaataatcgt accgttgcgc gtcaactgta cgccgacgcc
     7021 ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat ctgcggttgt
     7081 gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa gaggaattgg
     7141 cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa catcacaacc
     7201 gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact gaaaatcatc
     7261 gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac cagctttaat
     7321 agccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc acggcttatc
     7381 cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg gatagctact
     7441 gccactatat tttacggtcc cggaaaaatt agataacacg ccgccgtaag ccgagcctcg
     7501 ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa agatttgcgt
     7561 cgaaagatct acgaccaggt tttgccccac attcacgaca ggcgcaaggt ttacataaac
     7621 attagcgctg ccaccgccaa tagggatagc ggtaccattg gcggttttac aggcgaatga
     7681 ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaatag ctcgtttcat
     7741 tacaattatc tcttcgggtt cagctgtagg tataggtgat gctaatcact gcctgaatgg
     7801 ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg aagtgcgctg
     7861 attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat gtgttgccac
     7921 tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta taatatccgg
     7981 tgctgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc ggacaattag
     8041 tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacata agactgaaag
     8101 aataaagatc gccgagatct accgtggcat tggtggtgga aaccgtacac ggtttggcga
     8161 cgaccttacc gttcaccgtg atggtgacat cggctgcctg tatcgtcgca cttgcgagcg
     8221 ccaacattgc cgccaataca tacccacgtt tgcaccattt catgagcatc tccagttact
     8281 gatattcaag agtgaaggta gccgtagcat tgatatgccc cgcagtgaca ggcacctgtg
     8341 tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc agggtcgtcc
     8401 acgaaatcgc agacgatgga gcattaaggg gtatttgatt ttgctgctca ttcagaagct
     8461 gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg ttggcattgt
     8521 ggctatctgc aacgccggta aacccaactt ttacggcaga aacggcatta ccacagggtg
     8581 acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta aattgcttcg
     8641 ccgcgttttc catcagatca acagtaaaat tggttgattc agcggccaca ctacagccgt
     8701 tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaaacg cgacttaccg
     8761 ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg cccccttaac
     8821 gacattcagc tgatagctgg gttaataact gctgctgact ctctggtggc agttgataat
     8881 tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt cccgctaaag
     8941 gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc tggctactct
     9001 ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgagttagc gtcatgagca
     9061 gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacga gtgggaacaa
     9121 cgttagcgac cgcgttatct aaatcgacgt tatcagccaa ggtattggta tccagcgcca
     9181 ctctattttc ccgatattca gtggcataag gcagcacggc ataaccacgc cagtcggtac
     9241 gcacccccgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta acaagcacca
     9301 ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccaat accccaccgc
     9361 tgactccgta atagagctgc ttaatatcat cgctatggct gtaaccgata ttggcattgc
     9421 cgtaaccacc gcgataattc agcgtggcgt agcctgtact tccgctatta ccatcgcctc
     9481 ccccggcata gccggtttgc acgctataac tgaggttgtt gtcttccagc aacgtaccgt
     9541 atacaccagc cagattggtc atccgaccgt tgagatcgtg tgacatgctg tagctggcac
     9601 tggcatgtcg ccactgagat ttactgtcag aacgcagcca gtggctgaaa ggaatattga
     9661 cgttaagcgc taacatctga tcccgtcctt tttgccaggc gtttttcgtc aggctatagc
     9721 tgagcgtcca gttgatatct tcgaacgcag tatttaatcc agcctggaat tgctcatcga
     9781 cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt gatgtgcgcc
     9841 cgagttgctg agtaacggtg agctgtaatt tcccgcgttt gttataagcg aggttgtaat
     9901 agtcggtgaa tttcggctta acctgaataa ctccatcctg tgtttcgatg ttgtagccat
     9961 tcattcgact gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa taacggtaac
    10021 ccactaactg aatattcgtg cctgattcat tgagcgattt gttatagaga aaacgcaccg
    10081 attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc atatccacag
    10141 acagagcgcc cagtgcctcc atgtttttcc cgataccgaa attaaaagca cgataacgat
    10201 ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg agtaatgtac
    10261 tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct cctgccgtaa
    10321 tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag ggtacggtaa
    10381 aaatctgcgt gctgccgtca gcctctttga tcgttacctg caagtcacca ctattacctg
    10441 cggcatagat atcgttgatg gtaaaaggcc ccggtggcac cgtactatta taaatgccat
    10501 atccattttg tttaatagtg acctgtgcag taccacgagc aataccgtgg atcaccgggg
    10561 caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt gcgccgcgaa
    10621 agttaatacc atcgaaaata tcgccctggg tataaccatc acccaacgtc agccgggaac
    10681 gtaaaggaat tatgtctcgc tcaatccagg taatgatatg ctgccattta tttttgctac
    10741 ctgatgaact gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa cgccacgcac
    10801 caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc ccaatccg
//
LOCUS       DAZOYF010000134        10682 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZOYF010000134 DAZOYF010000000
VERSION     DAZOYF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.135"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            12..2264
                     /locus_tag="ACHUOF_003191"
     CDS             12..2264
                     /locus_tag="ACHUOF_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR0612254.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            2600..3532
                     /gene="glaH"
                     /locus_tag="ACHUOF_003192"
     CDS             2600..3532
                     /gene="glaH"
                     /locus_tag="ACHUOF_003192"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR0612255.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3552..4820
                     /gene="lhgO"
                     /locus_tag="ACHUOF_003193"
     CDS             3552..4820
                     /gene="lhgO"
                     /locus_tag="ACHUOF_003193"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR0612256.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4843..6291
                     /gene="gabD"
                     /locus_tag="ACHUOF_003194"
                     /pseudo
     CDS             4843..6291
                     /gene="gabD"
                     /locus_tag="ACHUOF_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            6299..7585
                     /gene="gabT"
                     /locus_tag="ACHUOF_003195"
                     /pseudo
     CDS             6299..7585
                     /gene="gabT"
                     /locus_tag="ACHUOF_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            7823..9223
                     /gene="gabP"
                     /locus_tag="ACHUOF_003196"
     CDS             7823..9223
                     /gene="gabP"
                     /locus_tag="ACHUOF_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR0612257.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9244..9906
                     /gene="csiR"
                     /locus_tag="ACHUOF_003197"
     CDS             9244..9906
                     /gene="csiR"
                     /locus_tag="ACHUOF_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR0612258.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9907..10356)
                     /gene="kbp"
                     /locus_tag="ACHUOF_003198"
     CDS             complement(9907..10356)
                     /gene="kbp"
                     /locus_tag="ACHUOF_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR0612259.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10440..10598)
                     /locus_tag="ACHUOF_003199"
     CDS             complement(10440..10598)
                     /locus_tag="ACHUOF_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR0612260.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2701 a         2646 c         2749 g         2586 t
ORIGIN      
        1 ggaacgttat tatgttttct ataaaaccag gacctggaaa tttacctatc gacaacccca
       61 cattgttatc atggaacatt actgatgggg atctaaattc caaatttaat acattagaat
      121 atctaaactg tataacaaat attattaatg cttgtggagt ttatcctcag gatttaaaag
      181 acagagaaat tatatcaact tttcacgcag aaaaagtcat taatgatctg ttaaaaaacg
      241 attataaaat ttccctttct ccagatacaa cttaccgaga gctgaataaa gctgcacaga
      301 gtagcattac agcgccagac aggataggag aagggaaaac atgggtttat caacgagata
      361 caatggttga aagaggtgat aacagtggtg ttcatcagta tggtccagct gaacatttca
      421 ctcacattat atctgacaaa ccgtccccaa aagataagta tgttgcatat gctattaaca
      481 ttcctgacta tgagctagca gccgatgtat ataatattaa cgtgacgtca ccttccggac
      541 agcaagaaac atttaaaata ctaatcaatc cagaacatct acggcaaaca cttgagcgta
      601 aatctcttac tgctgttcag aaatcacaat gtgaaatcat cacccccaaa aaacctggcg
      661 aagcgattct tcatgctttt aatgccacct accagcaaat cagggaaaat atgtctgagt
      721 ttgcacgttc ccattatggg tatatacaaa tccctccagt gacaactttc cgcgccgacg
      781 gaccagaaac tcccgaagaa gaaaaaggtt actggtttca cgcttatcaa cccgaagatc
      841 tttgtaccat ccataatcca atgggggatt tgcaggattt tatcgcattg gttaaagatg
      901 ctaaaaaatt tggtatcgat atcattcctg attatacctt taactttatg ggaatcgggg
      961 gaagtggtaa aaatgacctg gattatccct ctgctgatat acgagcgaag atcagtaaag
     1021 atatagaaag tggtatccct ggctattggc aaggtcaggt tttgattcca ttcattaaag
     1081 atccagtaac aaaagaacgt aaacaaatcc atccagaaga tatacatctc actgcaaaag
     1141 acttcgaagc aagtaaagat aacatctcta aggatgaatg gaaaaacctc catgcattaa
     1201 aagaaaagag tttaaatgga atgcctaaaa caacccccaa aagtgaccag gttattatgt
     1261 tgcaaaatca atacgttcgt gaaatgcgaa aatatggcgt acgaggttta cgttatgatg
     1321 cagcaaaaca ctcaaaacat gaacaaatag aaagatcaat aaccccaccg cttaaaaatt
     1381 ataatgagcg attacacaat actaacttat ttaacccaaa atatcatgaa aaaaccgtta
     1441 tgaattacat ggaatatctg gtaacttgtc agttgaatga agaacaaatg tcatcgctgc
     1501 tttatgaaag agatgattta agcgccattg atttttcatt gctcatgaag acgataaaag
     1561 ccttttcatt cgggggagat ctcctaaccc ttgcatcaaa accgggttcc acaatctcaa
     1621 gcatcccatc agaaagacgg atattgatta acattaacca cgattttcct aacaatggca
     1681 atcttttcaa tgactttcta tttaaccatc aacaagatga acaattagca atggcatata
     1741 tggccgctct cccgttcagc aggcctttag tttactggga tggccaggta ttaaaatcaa
     1801 cgactgaaat taaaaattat gatgggccca cgcgtgtcgg tggtgaggcg tggcttaata
     1861 aaggttgctc tacctatcag cagctctaca atgaattcca cgcattatat atagataaag
     1921 caggaatatg gagcgcattt gagggtgtat ttgcaactaa aaacgttctg gcctttagtc
     1981 gtggggattc tgtgaacatt aatcactctc ctcatgatgg actagttata ataaataaag
     2041 gaaacgaaga agttgaaggt acctggccta acaaattgca acctggaata tataaaaaca
     2101 tggggagtaa tagcgttaac attattatta ataatacccg aaaaattatc cccccttgta
     2161 aagtatttac gcttagaggc ggatctctaa atatcaatat tcctaggcgt agcgctcttc
     2221 ttttagggaa aacaggagaa ccgccgaact atctctattt ataatttaat gttatatctg
     2281 ccccgataaa acggggcaga taatatgttt agtttactaa cggtcatttt gtagtgaagc
     2341 catttactgt tttttatcga ccagataatc tgttctctaa tgttaactcc ccctaacctg
     2401 ttgctttagt tattcatttc ctgtctcact ttgccttaat accctacgtt aaatgttact
     2461 aatttgttgc ttttgatcac aataagaaag caatatgtcg cttttgtgcg catttttcag
     2521 aaatgtagat atttttagat tatggctacg aaatgagcat cgccatgtca ccctacatct
     2581 cataagagga tcgcttctga tgaatgcact gaccgccgta caaaataacg ctgtcgattc
     2641 aggccaggac tatagcggat tcactctcat cccgtcggcg caatccccgc gtctgctgga
     2701 actcaccttc accgaacaga cgaccaaaca gtttctcgaa caggttgccg aatggcccgt
     2761 gcaggcgctg gagtacaaat cgtttctgcg ttttcgggta ggcaaaattc ttgacgatct
     2821 gtgtgcgaat cagctgcaac cattgctgtt gaagaccctg ctaaaccgtg ctgaaggtgc
     2881 gctgttgatc aatgcggtag gtatcgatga tgtggcgcag gcagatgaga tggtgaagct
     2941 ggcaacggcg gtagcgcatc tgattggtcg ctcaaatttc gatgcgatga gcggtcagta
     3001 ttacgcgcgt ttcgtggtga aaaatgtcga taactcagac agctatctgc gtcagccgca
     3061 ccgcgtgatg gaactgcaca acgacggcac gtatgtcgaa gagatcaccg attacgtgct
     3121 gatgatgaaa atcgatgact gggaacatct ggaccactat ttccgccatc cactggcgcg
     3181 tcgcccgatg cgctttgccg cgccgccgag caaaaacgtc agcaaagatg ttttccatcc
     3241 agtgttcgac gttgatcaac agggtcgccc ggtgatgcgc tatatcgacc agttcgtcca
     3301 gccaaaagat tttgaagaag gcgtgtggtt gagcgagctt tccgatgcca ttgaaaccag
     3361 caaaggcatt ctttctgtac ccgttcccgt tggcaaattc ctgttgataa acaacctgtt
     3421 ctggctgcac ggtcgcgacc gctttactcc gcacccggat ctgcgccgtg aactgatgcg
     3481 tcagcgtggc tatttcgctt acgccactca ccactaccag acgcatcagt aagcgcaaag
     3541 gaattgagcg gatgtatgat tttgtgatta ttggcggcgg catcatcggc atgtcgaccg
     3601 ccatgcaact gattgatgtt tatccggatg cccgcattgc gttgctggaa aaagagtccg
     3661 gcccggcctg tcaccagacg ggccacaaca gcggcgtgat ccatgccggg gtctattaca
     3721 cgcccggcag cctgaaggcg cagttttgcc tggcggggaa ccgtgccacc aaagcctttt
     3781 gcgatcaaaa cggcattcgc tatgacaact gcggcaagat gctggtcgcc acgtctcagc
     3841 tcgaaatgga acggatgcgc gccttatggg aacgcacagc ggcgaacggt atcgagcgcg
     3901 agtggataaa cgccgtggaa ctgcgcgagc gcgaaccgaa tatcaccggg cttggtggca
     3961 tttttgtgcc gtccagcggc attgtcagct accgcgaagt gacggcggca atggcaaaaa
     4021 tcttccaggc cagaggcggc gagattattt ataacgccga agtcagcgcc ctcagtgagc
     4081 ataaaaacgg cgtggtgata cgcacccgtc agggcggtga atatgaagca tcgacgctga
     4141 ttagctgttc cgggctgatg gctgaccggc tggtgaaaat gctcggcctc gaaccgggct
     4201 ttattatctg cccgttccgt ggcgagtatt tccgccttgc gccggagcat aaccagattg
     4261 ttaaccacct gatttacccc attcccgacc ccgcgatgcc atttttgggc gtgcatctca
     4321 cccgcatgat cgacggcagc gtgaccgtcg ggccaaacgc ggtgctggct ttcaaacgcg
     4381 aaggctatcg caagcgcgac ttctcattta gtgacacgct ggagattttg agctcgtcgg
     4441 ggattcgccg ggtgctgcaa aaccatctac gctcaggact gggcgagatg aaaaactcgc
     4501 tgtgcaaaag cggctatctg cggctggtgc aaaagtattg tccccggctt tcgttaagcg
     4561 atctccagcc ctggcccgcc ggtgtgcggg cgcaggcagt atcgccggac ggcaagctga
     4621 ttgacgattt tctgtttgtc accaccccac gcacgatcca cacctgcaat gcgccctccc
     4681 cggcagcgac atcagcaatt cctattggtg cgcatattgt cagcaaggta caaacgctgt
     4741 tggcaagcca gagtaacccc ggacgcacgc tgcgagcggc acgtagtgtg gatgccttac
     4801 acgccgcatt taatcaataa cctttgaaaa caggacgtaa caatgaaact taacgacagt
     4861 aacttattcc gccagcaggc gttgattaac ggggaatggc tggacgccaa caatggtgaa
     4921 gtcatcgacg tcaccaatcc ggcgaacggc gacaagctgg gtagcgtgcc gaaaatgggc
     4981 gcagatgaaa cccgcgccgc tatcgacgcc gccaaccgcg ccctgcccgc ctggcgtgcg
     5041 ctcaccgcca aagaacgcgc caccattctg cgcaactggt tcaatttgat gatgcagcat
     5101 caggacgatt tagcgcgcct gatgaccctc gaacagggta aaccgctggc tgaagcgaaa
     5161 ggcgaaatca gctacgccgc ctccttcatt gagtggtttg ctgaagaagg caaacgtatt
     5221 tatggcgaca ccattcctgg tcatcaggcc gataagcgcc tgattgttat caagcagccg
     5281 attggcgtca ccgccgccat cacgccgtgg aactttccgg cggcgatgat tacccgtaaa
     5341 gccggtccgg cgctggcggc aggctgcacc atggtgctga agcccgccag tcagacgccg
     5401 ttctctgcgc tggcgctggc ggaactggcg attcgcgcgg gcattccggc tggcgtgttt
     5461 aacgtggtca ccggttcggc aggcgtggtc ggtaatgaac tgaccagcaa cccgctggta
     5521 cgcaaactgt cgtttaccgg ttagaccgag attggccgcc agttaatgga acagtgcgcg
     5581 aaagacatca aaaaagtgtc gctggagctg ggcggcaacg cgccgtttat cgtctttgac
     5641 gatgccgatc tcgataaagc cgtggaaggc gcgctggcct cgaaattccg caacgccggg
     5701 caaacctgcg tctgcgccaa ccgtttatac gtgcaggacg gcgtgtatga ccgctttgcc
     5761 gaaaaattac agcaggcggg gagcaaactg cacatcggcg acgggctgga gaaaggcgtc
     5821 accatcgggc cgctgatcga tgaaaaagcg gtagcaaaag tggaagagca tattgccgat
     5881 gcgctggaga aaggtgcgcg tgtggtttgc ggcggtaaag cgcacgaacg cggcggcaac
     5941 ttcttccagc caaccattct ggtggacgtt ccggccaacg ccaaagtgtc gaaagaagag
     6001 acgttcggcc ccctcgcccc actgttccgt tttaaagatg aagccgatgt gattgcacaa
     6061 gccaatgaca ccgagtttgg tcttgccgcc tatttctacg cccgtgattt aagccgcgtc
     6121 ttccgcgtcg gcgaggcgct ggagtacggc atcgtcggca tcaataccgg cattatttcc
     6181 aatgaagtgg ccccattcgg cggcatcaaa gcctcgggtc tgggtcgtga aggttcgaag
     6241 tatggcatcg aagattactt agaaatcaaa tatatgtgca tcggtcttta actggagaat
     6301 gcgaaggaac agcaataaag agttaatgca gcgccgcagt caggcgattc cccgtggcgt
     6361 tgggcaaatt cacccgattt tcgctgaccg cgcggaaaac tgccgggtgt gggacgttta
     6421 aggccgtgag tatcttgatt tcgcgggcgg gattgcggtg ctcaataccg ggcacctgca
     6481 tccgaaggtg gtggccgcgg tggaagcgca gttgaaaaaa ctgtcgcaca cctgcttcca
     6541 ggtgctggct tacgagccgt atctggagct gtgcgagatt atgaatcaga aggtgccggg
     6601 cgatttcgcc aagaaaacgc tgctggttac gaccggttcc gaagcggtgg aaaacgcggt
     6661 aaaaatcgcc cgcgccgcca ccaaacgtag cggcaccatc gcttttagcg gcgagtatca
     6721 cgggcgcacg cattacacgc tggcgctgac cggcaaggtg aatccgtact ctgcgggcat
     6781 ggggctgatg ccgggtcatg tttatcgcgc gctttatcct tgcccgctgc acggcataag
     6841 cgaggatgac gctatcgcca gcatccaccg gatcttcaaa aatgatgccg cgccggaaga
     6901 tatcgccgcc atcatgattg agccggttca gggcgaaggc ggtttctacg ccgcgtcgcc
     6961 agcctttatg cagcgtttac gcgctctgtg tgacgagcac gggatcatgc tgattgccga
     7021 tgaagtgcag agcggcgcgg ggcgtaccgg cacgctgttt gcgatggagc agatgggcgt
     7081 ggcgccggat cttaccacct ttgcgaaatc gatcgcaggc ggtttcccgc tggcgggcgt
     7141 caccgggcgc gcggaagtga tggacgctgt tgcaccgggc ggtctgggcg gcacctatgc
     7201 cggtaacccg attgcctgcg tggctgcgct ggaagtgttg aaggtgttcg agcaggaaaa
     7261 tctgctgcaa aaagccaacg atctggggca gaagctaaaa gacggattgt tggcgatagc
     7321 cgaaaaacac ccggagatcg gcgacgtacg cgggctggga tcgatgattg ccatcgagct
     7381 gtttgaagat ggcgatcaca gcaaaccgga cgcaaaactc accgccgaga tcgtggcacg
     7441 cgcccgcgat aagggcctga ttcttctctc ctgcggcccg tattacaacg tgctgcgcat
     7501 ccttgtacca ctcaccattg aagacgctca gatccgtcag ggtctggaga ttatcggtca
     7561 gtgttttgat gaggcgaagc agtagcaccg tccccatgca ggaggcgacg ctgcgcgtct
     7621 tgtccggcct acgagaatcc aagtcggata aggcgtttac gccgcatccg acaatctgta
     7681 cgtgaacagg aataaatcta tgtcggccgg ataaggcgga gccgctctcc ggcaaaaaga
     7741 atcaataaca attaaacgcg tgacccgacg cgggaaatgt cggggcgctc tcccaagtga
     7801 cacactttcg agaggattca ggatggggca atcatcgcaa ccacacgagt taggcggcgg
     7861 gctgaaatca cgccacgtca ccatgttgtc tattgccggt gttatcggcg caagtctgtt
     7921 tgtcggttcc agcgtcgcca tcgccgaagc ggacccggcg gtattactgg cctatctgtt
     7981 cgccggatta ctggtggtta tgattatgcg gatgctggcg gaaatggcag ttgccacgcc
     8041 cgataccggt tcgttttcca cctatgccga taaagccatt ggccgctggg cgggctatac
     8101 catcggctgg ttgtactggt ggttttgggt actggttatc ccactggaag ccaacatcgc
     8161 cgccatgatc ctgcactcgt gggttccagg cattcccatc tggttatttt ccctcgtcat
     8221 taccctcgcc ttaactggca gtaatttatt aagcgttaaa aactacggcg aatttgagtt
     8281 ctggctggcg ctgtgcaaag tcatcgctat cctggccttt attttccttg gtgcagtcgc
     8341 aattagcggt ttttaccctt atgccgaagt gagcgggatc tcaagattgt gggatagcgg
     8401 cggctttatg cccaacggtt tcggtgcggt attaagcgcg atgttgatca ccatgttctc
     8461 gtttatgggc gcagaaattg tcaccattgc cgccgcggaa tccgacatgc cggaaaaaca
     8521 tattgtccgc gccaccaact cggttatctg gcgtatttct atcttctatt tgtgctctat
     8581 ttttgtcgtc gtggcattaa taccgtggaa tatgccgggg ctgaaagccg ttggatctta
     8641 tcgctcggtt ctggaattgc tcaatattcc ccatgcgaaa ttaatcatgg actgtgtgat
     8701 attactttcc gtaaccagtt gcctgaactc ggccctgtat accgcctcaa ggatgctcta
     8761 ctccttaagc cgtcgcggtg atgctcccgc ggtaatgggt aaaatcaacc gcagtaaaac
     8821 cccgtacgtg gcggtgttac tctccaccgg cgcagcattc ttaacggtgg tggtgaacta
     8881 ttacgcgcct gcgaaggtat ttaaatttct gatcgacagc tccggcgcta tcgccctgct
     8941 ggtttattta gtcatcgccg tttcacagtt gcggatgcgc aaaattctgc gagcagaagg
     9001 aagcgaaatt cgcttgcgaa tgtggcttga tccctggctc acctggctgg tcatcggctt
     9061 tattaccttt gtgttggtag tgatgctatt ccgtccggcg caacagttag aagtgatctc
     9121 caccggctta ttagcgatag ggattatctg taccgtgcca attatggctc gctggaaaaa
     9181 gctggtattg tggcaaaaaa cacccgttca taatacgcgc tgataatgat caggagtcac
     9241 accatgacca ttacgtctct ggatggctat cgctggctga agaacgatat tattcgcggt
     9301 aattttcaac cggatgaaaa attacggatg agtttactga catcgcgtta tgcacttggc
     9361 gttgggccgt tgcaggaagc actttctcaa ctggtggcgg aacggctggt cacggtggtg
     9421 aatcaaaaag ggtatcgggt ggcgtctatg tcagagcagg agctgctcga tattttcgac
     9481 gcccgcgcca atatggaagc gatgctggtc agtctggcga ttgcccgcgg tggcgatgaa
     9541 tgggaggcag acgttctcgc aaaagcgcat ctgctcagta agctggaagc ctgtgacgcc
     9601 agcgagaaaa tgcttgatga gtgggatctg cgtcatcagg cgtttcatac agcaattgtg
     9661 gcgggctgtg gttctcacta tttgctgcaa atgcgtgaac ggttgtttga tctggcggcg
     9721 cgttatcgat ttatctggct gcggcgaacg gtgctttcgg tggaaatgct ggaggataaa
     9781 cacgatcagc accagaccct gactgcggcg gtactgccgc gagatacagc gcgcgccagt
     9841 gagttaatgc gccagcattt actgacgcca attcccatta tccagcaggc gatggctggc
     9901 aattaactac tcttccggaa tacgcaacac ttgccccgga taaattttat ccgggctttt
     9961 tagcatcggt ttattcgctt cgaagatttt attgtacaga ttagcgttac cgtagacctg
    10021 tttggaaatg gcactcagag tgtcgccaga cttaacggta taaaactggc tggcagtggc
    10081 tggtgtcgcc gttttcacct gatcatcaac actggcaata ccggaaatat tccccaccgc
    10141 aacaaggatt ttctccttcg cctcctgact caggccgtca ccagtgaccg tcgctttgcc
    10201 gtcggcaatt tgaatgttca ctttatcggc atcaggtata ccggttttgc tcagatgctc
    10261 ctgcaccttc ttcgcctgat cgtctttatc gtgctgacct gtaaccgcat cccagagttt
    10321 ttctccggca tctttaacaa aattgaacag acccatagct acctcattcg ttaacggaaa
    10381 cactcagaaa gtgtagcaga ggaacgcggc gcgttaacgc cgcgggggtg agcacaggtt
    10441 taatcgcggg tttgcaccca gaacgcgtga atcaaaccgg ggatataccc aagcagtgtc
    10501 agcagaatat taataatgaa cgcccaaccg aaccctttac cgagcagcac gccgatcggc
    10561 ggcagaatga tggtgatgac gattctccag aaacccatat gtactcccta taagaaaatt
    10621 actcattgtt taaaaagaga ttttatctct taagcgtagt aattttagcg gagcctgcca
    10681 gt
//
LOCUS       DAZOYF010000135        10673 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZOYF010000135 DAZOYF010000000
VERSION     DAZOYF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.136"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1817)
                     /gene="selB"
                     /locus_tag="ACHUOF_003200"
     CDS             complement(<1..1817)
                     /gene="selB"
                     /locus_tag="ACHUOF_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR0612261.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLR"
     gene            complement(1814..3205)
                     /gene="selA"
                     /locus_tag="ACHUOF_003201"
     CDS             complement(1814..3205)
                     /gene="selA"
                     /locus_tag="ACHUOF_003201"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR0612262.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3303..3911)
                     /locus_tag="ACHUOF_003202"
     CDS             complement(3303..3911)
                     /locus_tag="ACHUOF_003202"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR0612263.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3982..5067)
                     /locus_tag="ACHUOF_003203"
     CDS             complement(3982..5067)
                     /locus_tag="ACHUOF_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0612264.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5070..5432)
                     /locus_tag="ACHUOF_003204"
     CDS             complement(5070..5432)
                     /locus_tag="ACHUOF_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR0612265.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5969..7882
                     /gene="mtlA"
                     /locus_tag="ACHUOF_003205"
     CDS             5969..7882
                     /gene="mtlA"
                     /locus_tag="ACHUOF_003205"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR0612266.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            7977..9125
                     /gene="mtlD"
                     /locus_tag="ACHUOF_003206"
     CDS             7977..9125
                     /gene="mtlD"
                     /locus_tag="ACHUOF_003206"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0612267.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9125..9712
                     /gene="mtlR"
                     /locus_tag="ACHUOF_003207"
     CDS             9125..9712
                     /gene="mtlR"
                     /locus_tag="ACHUOF_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR0612268.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9724..9933)
                     /gene="yibT"
                     /locus_tag="ACHUOF_003208"
     CDS             complement(9724..9933)
                     /gene="yibT"
                     /locus_tag="ACHUOF_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR0612269.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10217..10579
                     /locus_tag="ACHUOF_003209"
     CDS             10217..10579
                     /locus_tag="ACHUOF_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR0612270.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         2564 a         2884 c         2752 g         2473 t
ORIGIN      
        1 cgcaataaat gatcatttcc acgacgacgc gtaaagccaa tgcggtcaaa atattccaga
       61 atctgaattg ccagctttcg gcctacgcct aagcgatcgc ggaaatccgc cgcgcaggtt
      121 gaaccacact cctgatcgag atcgcggatc atattggcaa attcgacaat ccgatcgtta
      181 cggtaataac gatctttgac gatcgcggta attattcctt gctgcgccgc ctggcgtagc
      241 gtcaggcgca ttgcctgctc gtcggtaccc gtctcttttg ccagatcacg cacccaccac
      301 ggctcgtcac caaacagcgg ctctgctttt tgccagatgg cctgctgctc ttcgctgaag
      361 cccgctttgt gatccggcag atgcagccaa ccgtgatgac tgaggatgtc gccgctttcg
      421 cgcatctttt caatcagcaa cagtactagc gcttcatctt ccattggcaa cgccatacgt
      481 cgcagacgtt cgcgcccagg cccaggttca tcgcgatgtt gctcatgata agtcgctaat
      541 gtgtcgagaa ttttccgttg ccagcgagcg gcaaccggcg cattcaacaa gctataacca
      601 gcctgaatat agccaggctg ttgcagcagt tcgcgcatcc cttcgccgtt gagctggcgc
      661 gcccaagcga aatcaggaag attaaccgcg ccgcgttgca ggtgaacggc taacgcttcg
      721 ctatcattct gtgccctggc gagggaagcc agccattgca gatactctgg tttacgttta
      781 ccgcgacgcg gcgggttaag catcacgacg cgcgctccgg ccagcgtgtt gcgggcagag
      841 atatcgcgca ataccaggcg gtcgttatct gccagccata acggggtgtc gaagaccagt
      901 tcagcaaggt tatcttccag cagtgaaacg cgtcccgtga cgtggctggc ggcgtggtga
      961 atatgcagcg gctgccactg ggtcagcggt gcatgggttt gaagctcgac aatcacccgt
     1021 gtgaacggct ctggcggcac atcggcaagc agccagtcgc cacggttaat ctgctctttt
     1081 tccgcatcac ccgcgatgtc aagcgcgata cgctgcccgg cattggcggt ttctgttggc
     1141 tggttttgcg catgcagcgc acgtacacgc atcggtttat ttacaccagt cagccagagt
     1201 gaatcgccta ccttcacttc cccgcttaac gccgtaccgg tgacgaccag cccggcacct
     1261 tttacggtaa atgcgcggtc aatcgcgagg cggaaactat gttggctggc gtgctcgcgt
     1321 tccggcaact gaagcagatg ctcgcgcagg gcatcaattc cccgaccttc ggttgctgcg
     1381 gtgataaaca gttttgcctc agcaaaaccg tattcccgca gaacctcctt tacctggcgt
     1441 tcaacctcat caacacgcgc ttcgtccacg cgatcggctt tggtcagcgc cactgtcagc
     1501 atcgggttac cggtcagctg caaaatcgcc agatgctcac gggtctgtgc catcacgccg
     1561 tcatcgcacg ccaccaccaa cagcgcgtga tcgataccac caacgcccgc cagcatgttg
     1621 gaaagaaact tttcatgacc gggaacgtcg ataaaaccag gcacgcgacc atccggctgc
     1681 ggccagtagg catagccgag atcgatggtc atgccgcgct ttttttcttc cggcagacgg
     1741 tcagcattta cgccagtaat cgcctgcaat aaggttgttt tgccgtggtc aacgtgtccg
     1801 gcagtcgcaa taatcatttc aacaacatct ccaaaaaccg ttgctcatct tcaaggcagc
     1861 gtaaatccag ccacaatcgt ccgtcataaa tacgaccaat caccggcact ggcaattcac
     1921 gccagcgggc ggctaatgac tcaaggtggc taccgcgtcc atcatggggt gtaaacgtta
     1981 atgccgcgct cggcaggcga tcaaccggca gcgaaccact gccaatctgc gaaagacatg
     2041 gcataacctg taccgcaaac tccgcgccgt aatgtgcgac aaggggagcc tgtaaacgtt
     2101 gtgcctggat ttgaatgacc tctgcgctgc gggtaagcag gcgcagggtc ggtaattttt
     2161 cactcagagc ttcagggtgt aaataaagac gcaacgtggc ttccagcgcc gcgagggtca
     2221 ttttatccgc gcgtaatgca cgcttcagcg ggtggctttg caggcgggcg atcatctctt
     2281 ttttaccaac gataatcccg gcctgcggcc cgcctaacaa cttgtcgccg gagaagctca
     2341 ccagactgac gcccgccgca atcaactcct gcggcattgg ctctttcggc aaaccgtact
     2401 ggctaagatc gaccagcgag ccactgccta aatcagtcac cactggaaca tccagctctt
     2461 tgccgagcgc caccagttca gcttcgtcta tcgctttggt gaacccctga atgctgtagt
     2521 tactggtatg tactttcatc aacagtgcgg tattttcatt caccgcctga cgataatcat
     2581 tcgcgtgcgt gcggttggtg gtccctactt cgtgtagggt gcagcctgcc tgacgcataa
     2641 catcgggaat acgaaacgcg ccgccaatct ccaccagttc gccgcgagat accaccacct
     2701 ctttcccgct ggcagtggcc gccaacatca ataacaccgc cgccgcattg ttattgacga
     2761 tacaggcatc ttccgccccc gtaatacggc acagcagctg cgccagcgcc cgatcgcgat
     2821 gtccgcgtcc ggcgtcgtcc agatcgtact cgagggtcac tggcgaacgc atagcctgcg
     2881 caacggcttc caccgcggct tccgcctgta aagctcgccc aaggttggta tgcagcacgg
     2941 ttcccgtcag gttgatcacc ggacgcagcg cgctctgcgc ctctttcgtc aaccgggcat
     3001 cgacttcttg cgcccagttt tcacaccacg caggcagcgt ctggctgcca cgaatcactt
     3061 ctcgcgcttc gtcgagcatc tgacgcaaca attccaccac gcgggtgtga ccataagtat
     3121 cacgcaaaga aaggaaggag ctatcgcgca ataagcgatc aatagccgga agttgactat
     3181 agagggaacg cgtttcggtt gtcataggaa acctggctga tcaaggccct ctcacacgga
     3241 gaagggcgtt taacataacc acggattgta acgtgagatg ggtcaggagg acatatcgcg
     3301 catcaagcct ttggcggttc ggtgcgagca aaactttcgc ggctaaacag gttttcgacc
     3361 agtttgacta agtgtgggcg gtcgacacac caacctggcg caacgcggcg gaaattgaga
     3421 tatccgacag cacaggcaat ggcgatagtc gccagattga ccgtatcggt tttgagtgtg
     3481 ccatcgacca gatatccttc cagcacatcc agactgcggt tgattttctc ccgctggcgt
     3541 aacaattcat cttcagactg ctgcgccgcc ggacgcgcct gttcacgcac cgataccagc
     3601 ccggcatcca taatgccatc cgccagcgcc tcaattttgc gcacccgcaa cgactccagc
     3661 ggatcgcgcg gcaacatcgc cggagcgaca ttcattaact caatatattc agcgatgatc
     3721 ggcgaatcaa accagcattc gccctcttcg gtcaccagca ccggcacttt ccctaacggg
     3781 ttaaattgcg ccacgccgtt atccgcgtta tagggcagtt cattaataaa ttcgaaagtt
     3841 atgccctttt ctaacaacag aataaaaagt ttgcgtacaa acgggctggt gtagctaccg
     3901 acgagtttca tgccgagtcc tttgtgcgag gaaaaatatc agtatggctc aggctcacgc
     3961 aaaacacagg tgatataagt ctcaatgatc caaataaaga taatgcatcc aactggtcat
     4021 tcttagcagc actttccgca tcaccgaaac atggctgaaa tggtcggagt aaaccgccac
     4081 ctgggcgtag atgccgtcgg gtaaggcatc gatatcatcg ttagggtcca gttcaatggt
     4141 tcccagcaca ccgtccgtgc cgggaacgac cgttaatgat tgcaataccc cctgcgcctg
     4201 ataagaaccg cctggcacga caggtaaaat actagtcaat ttgccgtgga acacctgccc
     4261 aggtagcgcg ttaaacacca cttccgcatc atcgccaggt ttcagacgta acagcgagtt
     4321 ttgccgaaat tgggcgacaa tttgccgttt ttgctcgggg atgaagacca tcaccggacg
     4381 cagcggcaag gcagctgcgt atgtacctgg gcggatcagt acctgagtga cgtagccatt
     4441 gcttggtgcg cgaatgacag tctgctcaag attatatttt gcttcagtaa gttgcgctct
     4501 taagctcaca atctgcgatt gctcgccgtt gaccatactg tcgagctgac tctggatctg
     4561 cgcctgctcc gccaccgagc ctttcactaa cgcatcctgc gcgaggaaat tttgccgcgc
     4621 atcgtcgatg tcacgttccg agaacggatt caccgccgcc tggctgcctt tcaggtaacg
     4681 ttgataattt ttaaacagac ggtcgcgctc cgctgaaacc tgggtggtgt tggcctgcgc
     4741 ttcagtgagc tgcgcacgca gcgtctttat attatgcgtc gccgtcatca ggtcagcctg
     4801 aagtctgtca actcgcgcct ggtaacgaac cgggtcgagc ttaaaaagca cctcgccctt
     4861 ttgaataagc tgattattct tgtcagtgac ttcagtaaca attcccgtca cctgtggcgt
     4921 gatagggatc gctatcactg ccttttgcgc ggtaaaagtg taagggtggt tgtagttcat
     4981 caacaaaatc aaaccactca ccagaaacac gcctcccagc gccgccgtcg ccagcgccac
     5041 gtaagttaaa acaatcaata gatccataat tagatctccg ggaacgtgga atagtcagtc
     5101 tttttctcgg cggcgagttg atgctccagc cgggaaattc ggtcagaaag tgcggcgatt
     5161 tccggttcag tcgctttctc ctgcgacgca acatgtgact gcatacccca gccacgctcc
     5221 ggttgataga gcgtcgccca gatccacaga aacggccaga taacatgcag agtaaacagg
     5281 ctcacccagc ccgccgtatg aatagcgtcg gcatgggggt gattgcgctt tttggcaatc
     5341 agataaggga tgtcatgtat cgcgatgatt ccataaaaaa tcaccagaaa gacgaagatc
     5401 agcactccca acgcgaaata gtttagaaac atatctgcct cggattcacg tttatcagtg
     5461 ttgttttttg gctggcagcc agaagggagt caggctgata ttttgacaat aatccgggtt
     5521 cgcgattctc gccataacac caaagaataa tttttagagg tgatgagttg cttagttaca
     5581 taacgattgt atgacgaagg cataacatgc tgtagatcac atcaggtgaa cgccgtaaga
     5641 aaatatcttg tgattcagat cacaaagatt caacaaacca tcaaaacaaa aatgtgacat
     5701 tactcacatt taaatgccat ttttagcgaa aatcaccgcc ttgtttcttt tttatacagg
     5761 cgttttgtga tgagcgtcac gtcaattacc tctctacccc ctatatttat gtgactgata
     5821 tcacacaaaa gcccgtcgac tggacagtta accgattcag tgccagattt cgcagtatct
     5881 acaaggtccg gctacctctg ccgccacatt aacaaaaaac ctcgggcttc cagcctgcgc
     5941 gacagcaaac ataagaaggg gtgtttttat gtcatccgat attaagatca aagtgcaaag
     6001 ctttggtcgt ttcctcagca acatggtgat gccaaatatc ggcgcgttta tcgcgtgggg
     6061 tatcatcacc gcgttattta ttccaacagg gtggttaccg aacgagacgc tggcgaagct
     6121 ggtcgggccg atgatcactt atctcctacc gctgctgatc ggttataccg gtggtaagct
     6181 ggtaggcggc aaacgtggtg gcgtagtcgg tgccattacc accatgggcg ttatcgtcgg
     6241 cgcagacatg ccgatgttcc tcggttctat gattgcaggt ccgctgggcg gctggtgcat
     6301 taagcacttc gaccgctggg tagacggtaa gatcaaatcc ggttttgaga tgctggtgaa
     6361 taacttctcc gcaggcatca tcgggatgat cctcgctatt ctggcattcc tcggcattgg
     6421 cccgattgtt gaagccctgt ccaaaatgct ggctgcgggc gttaacttca tggttgtcca
     6481 tgacatgctg ccgctggcgt ctatctttgt tgaaccggcg aaaatcctgt tcctcaacaa
     6541 cgccattaac cacggtatct tctcgccgct gggtattcag cagtcccatg aactgggtaa
     6601 atcaatcttc ttcctgattg aagctaaccc aggtccaggt atgggcgtgc tgctggcgta
     6661 catgttcttt ggtcgtggta gcgctaaaca gtctgcgggc ggtgcggcaa tcatccactt
     6721 cctgggtggt atccacgaaa tctacttccc gtatgtgctg atgaatccgc gtctgatcct
     6781 cgcagtcatc ctcggcggta tgactggcgt gttcacgctg actatcctgg gcggtggtct
     6841 ggtttctccg gcatctccgg gttctatcct tgctgtactg gcgatgacac caaaaggtgc
     6901 ttatttcgct aacatcgcgg gtgtgtgtgc ggcgatggct gtctccttcg ttgtctctgc
     6961 tattttgctg aaaaccagca aagtgaaaga agaagatgat attgaagcag caactcgtcg
     7021 tatgcaggac atgaaagctg agtctaaagg cacatctccg ctatctgcgg gcgatgtgac
     7081 taacgacctg agccacgtac gtaaaatcat cgttgcctgt gacgccggta tgggttcaag
     7141 tgcgatgggc gcaggcgtgc tgcgtaagaa aattcaggat gcaggtctgt cgcagatttc
     7201 ggtcactaac agcgcgatca acaacctgcc gccagatgtg gacctcgtca tcactcaccg
     7261 tgacctgacc gaacgcgcta tgcgccaggt tccgcaggca cagcatattt cgctgaccaa
     7321 cttcctcgac agcggcctgt acaccagcct aaccgaacgt ctcgttgctg cccagcgcca
     7381 tactgaaaac gaagtgaaag tgaaagacag tctgaaagac agctttgacg attccagtgc
     7441 taacctgttc aaactgggcg cggagaacat cttcctcggt cgcaaagcag caaccaaaga
     7501 agaagcgatt cgttttgctg gcgagcagct ggtgaaaggc ggttacgttg agccggaata
     7561 cgttcaggcg atgctggatc gtgaaaaact gaccccgact tatctgggtg agtctatcgc
     7621 ggtgccacac ggtacggttg aagcgaaaga tcgcgtactg aaaacgggcg tggtgttctg
     7681 ccagtacccg gaaggcgtgc gcttcggtga agaagaagat gacattgccc gtctggtgat
     7741 tggtattgca gcccgtaaca acgagcacat tcaggttatc accagcctga ccaatgcact
     7801 ggatgatgag tccgtcatcg agcgtctggc acacaccacc agcgtggatg aagtgctgga
     7861 actgctggca ggtcgtaagt aatccaatcc taccctctcc tcacggagag ggtttgggtg
     7921 agggaaaagc ctcaccccag ccctctcggg taaaaacatt gatgaaggtt aatactatga
     7981 aagcattaca ttttggcgca ggtaatatcg gtcgtggctt tatcggtaaa ctgctggcag
     8041 acgcgggtat ccaactgacg tttgccgatg tcaatcaggt ggtacttgat gccctgaatg
     8101 cccgtcatag ctatcaggtt catgtggtcg gtgaaaccga gcaggtagat accgtttccg
     8161 gcgtcaatgc tgtcagcagc attggtgatg atgtcgttga tctgattgct caggtcgatt
     8221 tagtcactac cgccgttggc ccggttgtgc tggaacgtat tgctccggct atcgccaaag
     8281 ggctggtgaa acgcaaagaa caaggtaatg aatccccgct gaacatcatc gcctgtgaaa
     8341 acatggtacg cggcaccacg cagctgaaag gtcatgtgat gaacgccctg ccggaagacg
     8401 ccaaagcgtg ggtagaagag cacgttggct ttgtcgattc cgccgttgac cgcatcgtac
     8461 cgccttcggc ttcggcaact aacgatccgc tggaagtgac ggtagaaact ttcagcgaat
     8521 ggattgtcga taaaacgcag ttcaaaggcg cactgccgaa catcccaggc atggagttaa
     8581 ccgacaacct gatggcattt gtcgaacgta aactcttcac cctgaacacg ggtcatgcta
     8641 taaccgcgta cctcggaaaa ctggccggtc atcagaccat tcgtgacgct attctcgacg
     8701 agaaaatccg cgcggtggta aaaggtgcga tggaagaaag tggtgcggta ctgatcaagc
     8761 gctacggctt tgacgcagac aaacatgcgg cgtacatcca gaaaatcctc ggtcgttttg
     8821 agaacccgta tctgaaagat gatgtagagc gcgtaggccg tcagccgctg cgtaaactga
     8881 gtgctggcga ccgtctgatc aagccactgc tcggtacgct ggaatacagc cttccgcaca
     8941 agaatctgat tcaggggatt gctggtgcaa tgcacttccg cagtgaagat gatccacagg
     9001 ctcaggaact ggcagcactg atcgctgaca aaggtccgca ggcggcgctg gcacagattt
     9061 ccggtcttga tgccaacagc gaggttgtat ccgaggcggt aaccgcttat aaagcaatgc
     9121 aataatggtg gaccaggcgc aggacaccct gcgcccgaat aacagattgt cagatatgca
     9181 ggcaacaatg gaacaaaccc aggccgttga aaaccgtgtg cttgagcgtc tgaatgctgg
     9241 caaaaccgtg cgaagctttc tgatcaccgc cgtcgagctt ttgaccgagg cggtaaatct
     9301 tctggtgctt caggtattcc gcaaagacga ttacgcggtg aagtatgctg tagaaccgtt
     9361 actcgacggc gacggtccgc tgggcgatct ttctgtgcgt ttaaaactca tttacgggtt
     9421 gggcgtcatt aaccgccagg aatacgaaga tgcggaactg ctgatggcat tgcgtgaaga
     9481 gctaaatcac gacggcaacg agtacgcctt taccgacgac gaaatccttg gaccctttgg
     9541 tgaactgcac tgcgtggcgg cgttaccacc gccgccacag tttgaaccag cagactccag
     9601 tttgtatgca atgcaaattc agcgctatca acaggctgtg cgatcaacaa tggtcctttc
     9661 actgactgag ctgatttcca aaatcagctt aaaaaaagcc tttcaaaagt aagcaacgtc
     9721 tgcttactgc ccctctacct gcttcggccg ataaagccga cgataatact ccagacgttg
     9781 tagatataac ggcacgcttt catcgggtat tccggacgga atcgcgttac ggggaggaag
     9841 ttttttcaga tactcccgga acgcctggct tgatgccatg aaatctacgg ctttatcgat
     9901 aagaagcgga acgttttcac ctaatttgcc catgatgata tctccgtatt accccgcgcc
     9961 gggaatgcgc ggccgccaat tttagtttag gttcccggta aagatgacat taaggaaaac
    10021 gtgctgaatc ctcaataatc atccgctgct gtttattatc tttttctata gatttcttaa
    10081 ttaatcaacg aattgcgtag tttttgagca tgttaatgat tacgttgcag aactattaca
    10141 atgtcatcag ttgtgaacaa agcacctggt cgcgcatact aggggctata aatttatctt
    10201 tatcagaagc catcacatga aagaagtcga aaaaaacgaa atcaagcgtc tcagcgatcg
    10261 cctggacgcc atccgccacc agcaggccga tctgtcgctg gttgaagccg cagacaaata
    10321 tgccgagctg gaaaaagaga aagccacgct ggaagcagaa attgctcgcc tgcgtgaggt
    10381 tcatagccaa aaactgagta aagaagcaca gaagctgatg aagatgccgt tccagcgcgc
    10441 aattaccaaa aaagagcagg ctgatatggg caagctgaag aaaagtgttc gcggactggt
    10501 ggttgtgcac ccaatgaccg cgctggggcg cgaaatgggc ctggaagaga tgactggatt
    10561 ttctaagact acgttttaag cccctcagac gactggttcg atcgtatcaa cccgcatctg
    10621 gcttgcctta gctggtcaat gcggggactc tcctgtctac tcgcctaatt acc
//
LOCUS       DAZOYF010000136        10659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZOYF010000136 DAZOYF010000000
VERSION     DAZOYF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.137"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="ACHUOF_003210"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="ACHUOF_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR0612271.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="ACHUOF_003211"
     CDS             complement(1614..1937)
                     /locus_tag="ACHUOF_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR0612272.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHUOF_003212"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHUOF_003212"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR0612273.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHUOF_003213"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHUOF_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR0612274.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHUOF_003214"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHUOF_003214"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR0612275.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHUOF_003215"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHUOF_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR0612276.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="ACHUOF_003216"
     CDS             7537..8574
                     /locus_tag="ACHUOF_003216"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0612277.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVLDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHUOF_003217"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHUOF_003217"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR0612278.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="ACHUOF_003218"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="ACHUOF_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR0612279.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHUOF_003219"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHUOF_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR0612280.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2647 a         2714 c         2739 g         2559 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 ctggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgtt
//
LOCUS       DAZOYF010000137        10503 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZOYF010000137 DAZOYF010000000
VERSION     DAZOYF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.138"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            2..1369
                     /gene="dcuC"
                     /locus_tag="ACHUOF_003220"
     CDS             2..1369
                     /gene="dcuC"
                     /locus_tag="ACHUOF_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0612281.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHUOF_003221"
     CDS             complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHUOF_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR0612282.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHUOF_003222"
     CDS             complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHUOF_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR0612283.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHUOF_003223"
     CDS             complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHUOF_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR0612284.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHUOF_003224"
     CDS             complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHUOF_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR0612285.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHUOF_003225"
     CDS             complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHUOF_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR0612286.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5324..5722
                     /gene="zapG"
                     /locus_tag="ACHUOF_003226"
     CDS             5324..5722
                     /gene="zapG"
                     /locus_tag="ACHUOF_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR0612287.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            5876..7223
                     /gene="degQ"
                     /locus_tag="ACHUOF_003227"
                     /pseudo
     CDS             5876..7223
                     /gene="degQ"
                     /locus_tag="ACHUOF_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            7313..8380
                     /gene="degS"
                     /locus_tag="ACHUOF_003228"
     CDS             7313..8380
                     /gene="degS"
                     /locus_tag="ACHUOF_003228"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR0612288.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACHUOF_003229"
     CDS             complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACHUOF_003229"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0612289.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            9815..10285
                     /gene="argR"
                     /locus_tag="ACHUOF_003230"
     CDS             9815..10285
                     /gene="argR"
                     /locus_tag="ACHUOF_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR0612290.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2583 a         2663 c         2617 g         2640 t
ORIGIN      
        1 aatgttcggc ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa
       61 aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt
      121 ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga
      181 catttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat
      241 tatggcggtg ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt
      301 gagcttgcta agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac
      361 ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggtc tgggtatgtt
      421 gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt
      481 ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaaacga actccatttt
      541 tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc ttccactacc agcttccggt
      601 cgcctcttgc gtcattatct cggtggcgat ctcccacttt ttcgtgcaac gcgcttttga
      661 caaaaaagat aaaaatatca atcacgaaca ggcagagcaa aaaactctcg ataatgtccc
      721 gccgctctat tacgccattt tacctgtgat gccgttaatc ctgatgctcg gctcgctgtt
      781 cctcgcccac gtcgggctga tgcagtcaga actgcatctg gtggtggtga tgttactaag
      841 tttgacggtg acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga
      901 cgatgtgcag gcgttttttg acggcatggg tacgcagttt gtcaacgtgg taacgctggt
      961 ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt ggcactgttg atgcggttat
     1021 caggggtgcg gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctagt
     1081 cattgccatt tgtgccattg tgatgggctc tggcaatgcg ccgtttatgt catttgccag
     1141 tcttattccg aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat
     1201 gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt actgcggtgg tggtcgttac
     1261 gtcaggaatt gcaggcgttt cgccttttgc ggtggtgaag cggacagcga tccccatggc
     1321 agtcggtttc gtggtgaata tgattgccac aatcacgcta ttttattaag tcattaaaaa
     1381 gacaaaacag gccgcctggg cctgttttgt attacttcac aacgcgtaat gccggtcgac
     1441 caccgcgtgg tggctgcgga ggttcatcgt caggatgagt gtcatcatcg tgatctggct
     1501 tgtcgccatc aataaccgac ataacggttt cgttgtctgc cgatgcctct tcatcattca
     1561 tgatgctggt atcttcatcg taggcagctt caggctcaaa catcgtgcct gcgccatttt
     1621 cacgggcgta gatagccagc acggcagcca gcggcacaga aacctgacgc ggaatgccac
     1681 caaagcgcgc gttaaagcgc acctcatcat tcgccagttc cagattgccg acagcacgcg
     1741 gcgcaatgtt gagtacgatt tgcccgtcac gcgcatattc cataggaacc tgcacgccag
     1801 ggagcgtcac atccaccacc aggtgcggcg tgagctggtt atccagcaac cactcataga
     1861 atgcacgcag cagataggga cgacgtggtg ttagctgtga caaatccata cagattaact
     1921 ccggcccaga cgcatttcac gttctgcttc agttaaagaa gcaaggaaag agtcacgctc
     1981 aaagacgcgg gtcatatagc ctttcagctc tttcgcaccc gggccgctga actcgatgcc
     2041 cagttgcggc agacgccaca gcagcggagc aaggtagcaa tcgaccaggc tgaactcatc
     2101 gctcaggaag tacggcttct gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag
     2161 ttgcttacgt gcggcatctg cttcagaagc tgaaccgttg atgatagtgt tcatcagcgt
     2221 gtaccagtct ttttcgatgc gatgcatgta cagacggctt tcaccgcgag ctaccgggta
     2281 aacaggcatc agtggcggat gcgggaaacg ctcatccaga tattccataa tgatgcgaga
     2341 ttcccacagg gtcagctcac gatccaccag ggtcggaacg ctctgattcg ggttgaggtc
     2401 aatcagatcc tgaggcggat tgtccttttc cacgtgttcg atctcgaaac ttacaccttt
     2461 ctcagccagc acaatgcgga cctgatggct atagatgtca gtaggaccgg aaaacagcgt
     2521 cattaccgaa cgtttgttgg cagcgacagc catgaaaacc tccaggtata gtcagaattt
     2581 ttactgctac cagccaccag gtggccagtc agaagttgtg ttacccaata aggaacgact
     2641 ctctttgttc gaaaatcaaa caaaaaatga gcaatacccg acatttgggc agaaaattgg
     2701 atgatagttt accagatttt gcgacctttg tggtgagtgg atgccggaaa tggggaaaaa
     2761 gagatgcgct ttagtctgaa atagttgact tagaccctta ttggcgatgt ggtttttgtt
     2821 ttacctgtct ttcagacggc agcaaaaagc aacttcccat tttttacgct gattcagaat
     2881 ttagccataa aaaaacccgc cgaagcgggt tttttcgcaa attgttttct gccgtagcag
     2941 aagccaatta acgtttggag aactgcggac gacgacgtgc tttacgcaga ccgactttct
     3001 tacgttcaac ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt tcagaacgca
     3061 gggactcgtc gtattccatc agagcgcggg tgataccgtg acggatcgca ccagcctgac
     3121 cagagatacc accaccttta acggtgatgt acaggtccag tttctcaacc atgtcgacca
     3181 gttccagcgg ctgacgaact accatgcggg cagtttcacg accgaagtac tgttccagag
     3241 aacgttggtt gattacgatt ttaccgttgc ccggtttgat gaaaacgcga gctgcggaac
     3301 ttttgcggcg accagtgccg tagtattgat tttcagccat tgcctataat cccgattaga
     3361 tgtcaagaac ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg taaactttca
     3421 gtttacggaa catagcacga cccagcgggc cttttggcaa catgccttta accgcgattt
     3481 caatcacacg ctcaggacgg cgagcaatca tctcttcaaa ggtcgcttgt ttgataccac
     3541 cgatgtggcc ggtgtggtga tagtacactt tgtcagtacg cttgttgccg gttacagcaa
     3601 ctttgtcagc gttcagaacg atgatgtaat caccggtatc tacgtgcgga gtatattccg
     3661 ctttgtgctt accgcgcagg cgacgagcca gttcagtagc cagacggccc agagttttac
     3721 cggtcgcgtc aacaacatac cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt
     3781 tcattaaaag cttacccaat aaatagttac acgttggtga acacccaaac gtcttcaatt
     3841 gttgaggttc acacgacaaa gtccggcaaa cctacccctt cgaatagcct atgccagcac
     3901 acaaaaagtt ttgggaaaaa aactttcttg taacgtgggg tcgcaggatt atagagaagt
     3961 cggggtcaaa gatcgacccc tttttgtgat ttgtggcagg ttttaacccg ccaaatgctc
     4021 gcgcttcaga tactcttcgc tttgcatctc ttgcagacgt gacaggcaac gctggaactc
     4081 aaacttcagc cgctcaccct gataaatttc atacagcggc acttctgcac tcaccactaa
     4141 tttgacatgg cgctcgtaaa actcatccac cagcgcaata aagcgccgcg cttcgctctc
     4201 catcaaccgc gtcatgactg gcacatcaaa caacattact gtgtgaaaca ggcgtgagag
     4261 cgcaatatag tcatgctgac tgcgggcgtc gacgcacagc gtagtaaaag aaaccgccag
     4321 cgtctggttc tcgacgccca ttgtcgccaa tggccgatgg ttgatttcta acgtcggtga
     4381 attttctcgt ttcacccccg ccagcgccaa ccatagttta tccatctgcg cccgggtttc
     4441 atcgttgagt ggcgaaagcc acagatgcgc ctgagtgagt gtacgcagtc gataatcaac
     4501 accagcgtcc acgttcatta catcacaatg ctgtttaatg gcatcgattg caggcagaaa
     4561 acgcgcacgt tgcaggccat ttcgataaag ttcgtccggc ggaatatttg acgtcgctac
     4621 cagggtaata ccgcgggtga acagggcttt catcagaccg ccaagtagca tggcgtcggt
     4681 aatatcagaa acaaaaaatt cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc
     4741 ggcaataatt tccagcggat cggtctgccc ctgtaaggca gttagctctt cgtgcacccg
     4801 cagcataaaa cggtgaaagt gcaggcgctg tttccgctct cccggcaggc tttgatagaa
     4861 aaggtccatc agccaggttt tcccgcgtcc tacaccgccc cacatatata agccacgcac
     4921 tggcgtatgc tttgtgtctt cgcgtttacc ccacagctta ccgacccgcg ccattagccc
     4981 actcgtcctg ggggctggtg gcctgctatt gatgagttct tgataaataa tttccaggcg
     5041 gctgacggcc tctttttgaa cgtcgtcggg ttgatggctg ccttcattga gcgccttcag
     5101 gtattgcgat gttggggtaa cgctttgcat gatcttattg ttattccttg aataatcggt
     5161 gcgccgttgt tcacggttga cgaaaaaaag gccgttctac actacgcgat atgcagtcgg
     5221 gattccactt ctgtggaatt aacggttata gtggcataat cagccgcagg catggagcct
     5281 gaagccaaca ccctacggaa acaaaagaca acgggagatg ttcatgacct gggaatatgc
     5341 gctaattggg ttagtcgtcg gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg
     5401 taaactacgc cagcaacagg cgttgcagta cgaactggaa aagaataaag ctgaactgga
     5461 cgagtatcgc gaagagctgg ttagccactt tgcccgcagc gcggaattac tggataccat
     5521 ggcgcacgac tatcgccagc tgtatcagca catggcaaaa agctccagca gcctgctgcc
     5581 ggaactgtct gctgaagcaa acccgttccg taatcgtctg gcagagtctg aagccagcaa
     5641 cgatcaggca ccggtgcagg tgcctcgcga ctattctgaa ggcgcatccg gcctgctgcg
     5701 tactggcgcg aagcgcgact aatttatttt tcgggcgcag ccattgcgcc ctcctcttct
     5761 ctccctcccc gactatcatt taatctggtg tctcattgtt agccgtctga aaattcaata
     5821 acatcaaact gttttgaatc tcttttctta tcattcaggt acgagagcag gaataatgaa
     5881 aaaacaaacc cagctgttga gtgcattagc gttaagtgcc gggttaactc tctcggcgtc
     5941 atttcaggcc gtcgcgtcga ttccaggcca ggttgccgat caggcccctc tccccagtct
     6001 ggctccaatg ctggaaaaag tgcttccggc agtggtgagc gtacgggtgg aaggaacggc
     6061 cagtcaggga cagaaaatcc cggaagaatt caaaaagttt tttggtgatg atttaccgga
     6121 tcaacctgca caacccttcg aaggtttagg ttccggtgtc atcatcaacg ccagtaaagg
     6181 ctatgtgctg accaacaacc atgtgattaa tcaggcacag aaaatcagta ttcagctcaa
     6241 tgatgggcgc gagtttgatg caaaactgat tggtagcgat gaccagagcg atatcgccct
     6301 gttacaaatt caaaacccga gcaaattaac gcaaatcgct attgccgact ccgataaatt
     6361 gcgcgtcggt gattttgccg tagcggtcgg taacccattt ggccttgggc aaaccgccac
     6421 ctctggcatt gtttccgcat taggccgcag cgggttgaat cttgaaggtc tggaaaactt
     6481 tatccagaca gatgcttcca ttaaccgcgg taactccggc ggtgcactat taaaccttaa
     6541 cggtgagtta attggcatca acactgcaat ccttgcgcct ggcggcggga gcgtcgggat
     6601 tggatttgcc atccccagta atatggcgcg aacactggcg cagcagctta tcgactttgg
     6661 tgaaatcaaa cgcggtttgt taggcatcaa aggcaccgag atgagtgccg atatcgccaa
     6721 agccttcaac cttgacgtgc agcgtggcgc gtttgtcagc gaagtgttgc caagttctgg
     6781 ctcagcgaaa gcgggcgtca aagcgggcga tattattacc agcctcaacg gcaaaccgct
     6841 gaatagcttt gctgagttgc gctctcgtat cgcgactaca gagccgggca cgaaagtgaa
     6901 gcttggcctg ctgcgtaacg gcaaaccact ggaagtagaa gtgacgcttg ataccagcac
     6961 ctcttcgtcg gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta aaagatggcg
     7021 gcaaaggtat taagatagat gaagttgtca aaggaagccc agctgctcag gctggcttgc
     7081 aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt gctgaaatgc
     7141 gtaaagtgct ggcggcaaaa ccggccatca tcgccctgca aattgtacgc ggcaatgaaa
     7201 gcatctatct gctgatgcgt taatgtcgta aaccgggcat caggctgacg tgtgatgtcc
     7261 ggttaactcg tggtatgctg ctgccgttcc cttttttaat gacgcctcca tcatgtttgt
     7321 gaagctctta cgttccgttg cgattggatt aattgtcggc gctattctgc tggttgccat
     7381 gccttcgctg cgcagcctta acccgctttc cactccgcaa tttgacagta ccgatgagac
     7441 gcctgccagc tataatctgg cggttcgccg cgccgcgcca gcggtggtta acgtttacaa
     7501 ccgtggtttg aacaccaact ctcacaacca gcttgagatc cgcaccctgg gatccggtgt
     7561 aatcatggat caacgcggtt atatcatcac caataaacac gtcatcaacg acgccgatca
     7621 gatcatcgtc gccttacagg atggacgtgt atttgaagca ttgctggtgg gatctgactc
     7681 tctaaccgat ctggcggtac ttaaaattaa tgccactggc ggtttaccta ccattccaat
     7741 taatgcacgt cgcgtaccgc acattggcga cgtagtactg gcgatcggta acccgtacaa
     7801 cctcgggcag accattaccc aagggattat tagtgccacg ggtcgaatcg gtctgaaccc
     7861 gaccgggcgg caaaacttcc tacaaaccga tgcttccatt aaccacggta actctggcgg
     7921 cgcgctggtg aactcgctgg gagaactgat gggcattaac acgctgtcgt ttgataagag
     7981 taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt tagcaaccaa
     8041 aattatggat aagctgatcc gcgatggtcg cgtgatccgc ggctacattg gtatcggcgg
     8101 acgcgagatc gcaccactgc acgcgcagag cggtggtata gatcaactgc aagggatcgt
     8161 ggttaatgaa gtgtcacctg acggcccagc ggcgaatgcg ggtattcagg tcaacgatct
     8221 gattatttcg gtggataaca aaccggccat ctctgctctg gagacgatgg atcaggtggc
     8281 ggaaattcgc cctggttcgg tgatcccggt tgtagtgatg cgtgatgata agcagttaac
     8341 gctgcaggtc accattcagg aatatccggc aaccaattaa gtcgagtgct caaaacaaaa
     8401 accggagtct gtgctccggt tttttattat tcgctaatca attacttatt aacgaactct
     8461 ttgcccaggg cgatatcttt cttgagcgta tccagcatac cttccagcgc gttctgttca
     8521 aatgcgctca gggtaccgat agatttacgc tcttccacgc cgtttttacc cagcagcagc
     8581 ggttgagaga agaaacgggc gtactgaccg tcgccttcaa cgtaggcaca ttcgacaacg
     8641 ccttgttcgc cctgcagtgc acgaaccaga gacagaccaa aacgtgcagc tgcctggccc
     8701 atagacaggg ttgcagaccc gccaccggct ttcgcttcaa ccacctcagt acccgcgttc
     8761 tggatacgtt tggtcagatc agccacttcc tgctcggtaa aactaacgcc aggaacctgt
     8821 gacagcagcg gcagaatggt aacaccagag tgaccgccaa taaccggcac ttcaacttcg
     8881 cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg aacgaatgat atccagcgtg
     8941 gtaacgccga acagtttgtt tttgtcataa acaccggctt ttttcagcac ttcagcagca
     9001 attgcaactg tggtgttaac cgggttagtg ataataccaa tgcacgcttt cgggcagttt
     9061 ttcgcaactt gctgtaccag gtttttcacg atgccggcgt taacgttaaa caggtcggaa
     9121 cgatccatac ccggtttacg cgctacgcct gcagagataa gaacgacatc tgcgccttcc
     9181 agcgccggag tcgcatcttc accagaaaaa cctttgattt tcacagcagt agggatatgg
     9241 ctcagatcga cggccacacc gggagtcact ggagcgatat catacagaga gagttctgaa
     9301 cctgaaggca gttgggtttt taacagtagt gcaagcgcct ggccaatacc gccagcagcg
     9361 ccgaggactg cgactttcat cctaaactcc ttattatatt gataaactaa gatatgttgc
     9421 tccgctgccg cgaccttaat ccacaaaatt gccgtttaca atgaccacat ctcaagaatg
     9481 tgtagtcacg caagtttagc gtttatgcat ttaattgccg taatcaggaa cctaacttac
     9541 gtaattaaca gtcgcttatg cattagcgca acattccaac aggtggtgac aatataccct
     9601 accgttcagc caaaacaaca tcaatttgat aacaattaat ttacttttaa acagaatttg
     9661 catgccgtga cgcaggcatg tttctcaata acgaaatttg ataaaatccc gctctttcat
     9721 aacattattt cagccttctt cagggctgac tgtttgcata aaaattcatc tgtatgcaca
     9781 ataatgttgt atcaaccacc atatcgggtg acttatgcga agctcggcta agcaagaaga
     9841 actagttaaa gcatttaaag cattacttaa agaagagaaa tttagctccc agggcgaaat
     9901 cgtcgccgcg ttgcaggagc aaggctttga caatattaat cagtctaaag tctcgcggat
     9961 gttgaccaag tttggtgctg tacgtacacg caatgccaaa atggaaatgg tttactgcct
    10021 gccagctgaa ctgggtgtac caaccacctc cagtccattg aagaatctgg tgctggatat
    10081 cgactacaac gatgcagttg tcgtgattca taccagccct ggcgcggcgc agttaattgc
    10141 tcgcctgctg gactcactgg gcaaagcaga aggtattctg ggcaccatcg ctggcgatga
    10201 caccatcttt accacccctg ctaacggttt caccgtcaaa gacctgtacg aagcgatttt
    10261 agagctgttc gaccaggagc tttaatctct gccccgtcgc ttctggcggc ggggaaaatg
    10321 ttgcttatcc ctctcattcc ccttctttct cctgcgatac atttaacgaa tagtgcgttt
    10381 taccgcgaca catcattcac aaaatgaata catacggtaa aaaaagcaca ttatgcaaaa
    10441 ttcattatct aattgaaaat actataatta ataataaata accgtgtttt ttatactttt
    10501 ttg
//
LOCUS       DAZOYF010000138        10464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZOYF010000138 DAZOYF010000000
VERSION     DAZOYF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.139"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            2..202
                     /gene="cspG"
                     /locus_tag="ACHUOF_003231"
     CDS             2..202
                     /gene="cspG"
                     /locus_tag="ACHUOF_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR0612291.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            213..401
                     /locus_tag="ACHUOF_003232"
     CDS             213..401
                     /locus_tag="ACHUOF_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR0612292.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            376..606
                     /gene="ymcE"
                     /locus_tag="ACHUOF_003233"
     CDS             376..606
                     /gene="ymcE"
                     /locus_tag="ACHUOF_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR0612293.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            596..769
                     /locus_tag="ACHUOF_003234"
     CDS             596..769
                     /locus_tag="ACHUOF_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR0612294.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(818..1891)
                     /locus_tag="ACHUOF_003235"
     CDS             complement(818..1891)
                     /locus_tag="ACHUOF_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFR0612295.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1993..4704)
                     /gene="torS"
                     /locus_tag="ACHUOF_003236"
                     /pseudo
     CDS             complement(1993..4704)
                     /gene="torS"
                     /locus_tag="ACHUOF_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4787..5815
                     /gene="torT"
                     /locus_tag="ACHUOF_003237"
     CDS             4787..5815
                     /gene="torT"
                     /locus_tag="ACHUOF_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR0612296.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5788..6480)
                     /gene="torR"
                     /locus_tag="ACHUOF_003238"
     CDS             complement(5788..6480)
                     /gene="torR"
                     /locus_tag="ACHUOF_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR0612297.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHELSADLLVTQ
                     HGEGYFLAADVC"
     gene            6567..7739
                     /gene="torC"
                     /locus_tag="ACHUOF_003239"
     CDS             6567..7739
                     /gene="torC"
                     /locus_tag="ACHUOF_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR0612298.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7739..10279
                     /gene="torA"
                     /locus_tag="ACHUOF_003240"
                     /pseudo
     CDS             7739..10279
                     /gene="torA"
                     /locus_tag="ACHUOF_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10276..>10464
                     /gene="torD"
                     /locus_tag="ACHUOF_003241"
     CDS             10276..>10464
                     /gene="torD"
                     /locus_tag="ACHUOF_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR0612299.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2523 a         2686 c         2789 g         2466 t
ORIGIN      
        1 aatgactggt ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga
       61 tgatggcagc aaagacgttt tcgtccattt caccgccatc cagagcaatg aattccgcac
      121 gctgaacgaa aatcagaaag ttgaattttc tattgagcag gggcaacgtg gccccgcggc
      181 agcgaacgtt gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc
      241 catgttgtca tggttcacag taccgtacat cggcattcga tgtgacggag cgaaaccctt
      301 tgggcgctaa gtgtattttt tgtaaatcga ctatgatcac ctttgataac gtcgcgctgc
      361 aaatacgcac tgaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcgtgga
      421 gcctttttta tatctgcctt atttttcttc aacgctgtat gtatagtaag cgataacctg
      481 ttgattattg aatctttcgg gaagatggct tataacattt cttacctgac cagggtaccg
      541 ggaaccaaca ccttactggc gtgttgctgt ctttcaagac cagacgaggt taacagtgaa
      601 tattgaagag ttaaaaaaac aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat
      661 cgccgagctg aacaagaata cagggaaaga agtctctgaa attcgcttca tcgcacgaga
      721 aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca
      781 tcaccccgtg cgaggtgatg ttccgcttgt tgctaattta gtgaccaatc attggcgctt
      841 gtggaattaa gcgtcggtac aattcctccg gcaccgggct ttgccatact cccgcataca
      901 ttgcgtaacc aatcaccgca aacataatcc ccagaaccag tagcgtcatt aaccagccag
      961 acaacgcaaa ggctttttta tttgctgcag gtttttgcag tgaaaaggtc aatgttgagg
     1021 ctaccggaca tgactctacg caagtcatac agccggtaca ttccactgtt cgtacctgaa
     1081 ttaatttatc taccgggatc cgtgatgggc acttttttgc gcatttgcca caatcgatac
     1141 aactttcggc attgcgacga atcttaaacg gcgacaatag cgaaaccacg cccatcagcg
     1201 cgccatatgg gcaaagataa cgacaccagg catggcgaat aaacaggctg gcaatcagca
     1261 aaacggtcac gctgattaat gtcgcggtcc ccatatgacg aaagaaatcg agcattttaa
     1321 cgtccatcac cacgctatag ggcgacaaca taaaatagtg aatcgcctga gcgggcatca
     1381 ataacgcgat atagagaaat aaactcaaca gcaaatactt cacgccgcgc agaggaatat
     1441 ccagccagcg gggaaggaca cattgccgac caaacagttt gttaccgaga tcgccgatta
     1501 attcagaaag cgtaccaacc gggcataacc atgagcaaaa ggcctttttg agtaatagac
     1561 tgatgacgat aaaagcgacc aataacagca tcgcggcggc gtggacggac ggtaactgac
     1621 ctgttgcaag gctatatttc agattcatca gaccggcaat cggtagccag ccttcgatac
     1681 ctcccggtct ggcgacaaat gtcgtactac ctgccgtttc gtaatagcgc acccaatacc
     1741 agaacgtgat ggcaatataa atattcattg ccaatagtaa gaattgcgtc gctttacgcc
     1801 aggtcgtggc attgcgccag tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc
     1861 gctgccagcg ggttcttttt ttctctgcca tgattttgcc agtccgttaa gttgcatacc
     1921 aaatgccact attctagtta ttcttaactg gctgatattg attcaaatcg cgttcaggtc
     1981 tttcttattc aatcatgctt ccagagcggc aacactgcgt gtaatttctt cgtgtggaag
     2041 gggggcagat aatggctgct gctccagttg tgcgcatagc tgactggcgc tacgcattcc
     2101 cagactcgag caactgcttt ttagctgatg cgcggcacgc tttattttct cgctgtcctg
     2161 gctggcgcgg gcaatgtcga tttcatcgag aagcggcagg gcatgttgtt taaataatgc
     2221 cagccattcg tggatcttct ccgtccccat taactgagcg tcttcattga gttgcgagac
     2281 atccagtggt agatcgttat tggcttgcag ttggagatag tgcgccagta actgaccgag
     2341 cacttcacgc ggcaccggtt tagggataat cccgcgaaat agtgaactgg tacgctggcg
     2401 cagcgtttcg tcaatgacat gggcgctaaa gccaatcaaa accagcgacg gatattgctg
     2461 tgccagttgc cgggcaaggg taatgccgtc gatatccggc agatcaaaat ccaccagtgc
     2521 ggcagcaaac ggttcgctat tttgcagtgt ctctaaagcc tgcgcggcat tgccaatagc
     2581 aacaacctgc gcaccactgg tgttcagcat ctcaacggta attcgctggg ttagcgggtt
     2641 atcttcaatt aacagcaaac gtaaaccgtc aagacgcacc gcctgattgg ctgtttttgg
     2701 cacgggtgca gtggcaacac gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg
     2761 cgtgctggtg gcgctcagtt cgccgcccat cgcctgggcc agacggctac tgatagtcag
     2821 ccccagcccg gtgccgccgc gtttgccgct tacctgcaca aatggctgga agatttctgc
     2881 cagtttcgcg ggatcaatac cgcagccgct gtcttccact tcgaccagcc attgctcgcc
     2941 atcagtgcga ctacgcagga taatgtgccc ttcgtcagta aaacgcaggg cattgctcaa
     3001 taggttggta atgacctgac gaatacgtcg tggatcgccc attaacgcgc tcggtacatc
     3061 atcggcaatt gccgttgcca ggcgaatcgg gcgacctttc acccgtccgc tcattaattg
     3121 cagggtactt tccagcagcg ggcgcggttc aaagggctca tcgctgactg aaacattttt
     3181 gccacctgct tcgatagccg aataatcgag aatatcgttg aggatggtca gcaacgattc
     3241 gccagagtcg gtaatcgcct gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc
     3301 cagcagttga gcagtgccga gaataccgta cagcggtgtg cggatctcat ggctcatcgc
     3361 cgtcagaaac gccgattttg cctggctggc tttttctgct tccgcccgtg cctgtcggtg
     3421 ttctatcacc agttcctgca attcagctgt acgcgctttg acctgcgccg ccagctgttc
     3481 gcggtggcga ttcagtgcat gaacactgct gcgaaacgca tccatcagcc gcccgatggt
     3541 atccagctcc cgtacgccag cggtttccgg gaaaggggag tcaatatcac cgtccagcag
     3601 ccgttgcagc gcctgcgttt gttcggcaag tggacgcgtg aatgagcgat aaaccacgcg
     3661 ccagaggatc ataatcagtg cgcaaagtga aaccatcccc agcaataaca ggctgtattg
     3721 cccgcgtgca ctggcttttt ccagatgtgc cagtccatgc tgattgtgca gctcaatggt
     3781 gtcgaccagc tgactgactt cgctactaaa ctgcgcgaac tgggcgatat tattttgggc
     3841 gagagtttgc aggtgattgc tgatttcact gtcctgctgg aacagcgcca acagatcgct
     3901 atattggcta acggtagtta acgttgttgc gacctgcgca cgaactcccg gatcttcaat
     3961 gcgtatttgc cgacgttgca gaattttcac cgcattattg agctgctttt ccagcgttgg
     4021 tgcatttttc tggatctgct ccagccccag attcatcacc atttgctgca cccgcagagc
     4081 gctaaggcgc agttcattca tctggttaac atactcaaga tcgatatcaa tcagccgatc
     4141 gagtgcactt tcagcagcct gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg
     4201 ggtcgctcca gcggaagttg tcgcattatt cgcctgacct tgctccaggc gtgcgatctc
     4261 atcggcggca gctatctgct gactgagttg ccgttgttgc tggcgtagtt gcagacgccg
     4321 ccccaccagt tccccttgct gacgtaatga acgggagatc tcctgctcct gttgttcaat
     4381 agcggtggta tcaaaacctt gttcccgtaa cgcttgcagc aacgcattaa tcttcaggct
     4441 ttatgcggtg agcattcgcc cctgcgcctg ccacatcttt tcgttatcgg cactggtcag
     4501 gttctgcgcg gcgaaaagtt cccaggcgct ggcttcgctc aactggcgcg ccatattcat
     4561 ggtaggaatc aatgcctgag tattgtcttt ttccacctgg ctgataaagc gcaggttgta
     4621 ccatcccacc agggtactgg tcagggttaa cagcgccatc agggcaaagc ccatccagag
     4681 tcttcgggtc agggttaaat tcacggtcgg tgcacttaag gtgaaaaagg ttgagtcgca
     4741 aagtggaatg catctagcat aaagccttat tattgatgag gctatcatgc gcgtactgct
     4801 atttttactt ctttcccttt tcatgttgtc ggcattttcg gctgataacc tgttgcgctg
     4861 gcatgatgcg cagcatttca cggtgcaagc ctctatgccg cttaaagcca aacgcgtatg
     4921 gaaactgtgc gcgctttatc ccagcctgaa agattcatac tggttatcgt tgaactatgg
     4981 tatgcaggag gctgctcgcc gctacggtgt ggatttaaaa gtgctggagg caggcggcta
     5041 cagccagttg gctacccagc aagcacaaat cgaccagtgt aaacagtggg gcgcagaggc
     5101 cattttgctc ggtagtagca cgacgtcatt tcccgacctg caaaagcagg tagcaaatct
     5161 gccggtgatc gaactggtaa atgctcttga tgatccccag gtgaaaagcc gcgttggtgt
     5221 accctggttt cagatgggct atcaacctgg gcgatatttg gtgcaatggg cgcacggtaa
     5281 accactgaac gtgctgctga tgcccggacc cgataacgcc gggggcagta aggagatggt
     5341 cgagggtttt cgcgcagcca ttgccggaag cccggtgcgt attgttgata ttgcgcttgg
     5401 tgataacgat attgaaatcc agcgcaacct gttgcaggag atgctggaac gccacccaga
     5461 aatcgacgtc gttgccggaa cggccattgc ggcagaggcg gcaatgggtg aagggcgtaa
     5521 cctgaaaacg ccgcttaccg tggtgtcgtt ttatctttca catcaggtgt atcgcgggct
     5581 gaagcgggga agagtgatta tggctgccag cgatcaaatg gtctggcagg gggaactggc
     5641 agttgagcag gccatcaggc aattacaggg gcaatcggtg tctgataatg tcagcccacc
     5701 gattttagtt ctgacgccga aaaatgccga ccgagaacat atccgccgct cgctgtcacc
     5761 ggggggattt cgtccggtct atttttatca gcacacatca gcggctaaga aataaccttc
     5821 accatgttgc gtcaccagta aatccgcgct gagttcatga cgtaaacgac gaattaacac
     5881 atcgacggtg cgcaggtcag ggttttccac ccgacgcgca gaaagcatac gcagcagacg
     5941 ctcacggctg agaatttcgc ccggattagt cacaaatgcc accaacattt catactctgc
     6001 gcgggtcagt ttaatcggct cgccatcccg ctccagcgta tggcgcgaca cattcaggca
     6061 ataacccgca aagcgatagc agtagtcctg agtgtgcggt tgagcttgtc gcgcgaggtc
     6121 gattcgccag agcagatttt tcacccgtac taccagttcg cgcagttcca gcggtttggt
     6181 gacgtaatcg tctgcgccca tttccagccc aacaatacgg tcaatccgat cgctgcgtcc
     6241 ggtaaccaga ataatcccca ccgttgagcg ttctcgcagg gcgcgggtta acatcaggcc
     6301 attttcatcg ggtaagttga tatccagcag aattaaatct accggctgat tctgcataat
     6361 ttcccgtagc ccggcaccgc tcgccgtaac ggaaacggta tacccctcct gagtgaagta
     6421 ggattgtaat cgcgcctggg taaccggctt atcttcaaca ataacaatgt gatgtggcat
     6481 cagagggttt tactcattct gttcatatct gttcatattt gctcattaag atcgcttcac
     6541 taaaccataa ttctacaggg gttattatgc ggaaactctg gaacgcgcta cgccgaccca
     6601 gtgctcgttg gtcggtactg gcgctggtcg caattgggat tgtgattggc attgcgctga
     6661 ttgtattgcc acacgttggg atcaaagtca ccagcacaac cgaattttgt gtcagttgcc
     6721 acagtatgca accggtgtat gaagaatata aacagtcggt gcatttccag aacgcctccg
     6781 gcgtgcgagc tgaatgccat gactgtcata tcccgccgga tattccaggc atggtgaagc
     6841 gcaaactgga agcgagcaac gacatttacc agacctttat tgcccactcc attgatacac
     6901 ctgaaaaatt cgaagccaaa cgcgcggaac ttgccgagcg tgaatgggcg cgaatgaaag
     6961 aaaacaactc ggcaacctgt cgctcctgcc ataactacga tgcgatggat catgccaggc
     7021 agcatcctga agcggcacgt cagatgaagg tggcagcgaa agataatcaa tcctgcatcg
     7081 actgtcataa aggtattgcc caccagttac cggatatgag tagcggcttc cgtaagcagt
     7141 tcgatgagct gcgcgccagt gctaatgaca gtggtgacac gctgtactct attgatatta
     7201 agccaattta tgcggcgaaa ggcgataaag aagcctctgg ttctctgctg cctgcttcgg
     7261 aagtgaaagt ccttaaacgt gacggcgact ggctgcaaat tgaaattacc ggctggacgg
     7321 aaagcgccgg acgtcagcgt gtactcaccc aattcccagg taaacgcatc tttgttgcct
     7381 cgattcgtgg tgatgtgcag cagcaggtga aaacgctgga gaaaaccacc gttgccgaca
     7441 ccaataccga gtggagcaag ttgcaggcca ccgcgtggat gaagaaaggc gacatggtga
     7501 acgatatcaa accgatctgg gcctatgcgg attcgttgta caacggcacc tgtaacgagt
     7561 gccacggcgc accggaaatc gcccactttg acgctaacgg ttggatcggc acgctcaacg
     7621 gcatgattgg ctttaccagt ctcgataaac gtgaagaacg cactttgttg aaatatctgc
     7681 aaatgaatgc gtctgacacc gcaggtaagg ctcacggcga taagaaggaa gaaaaataat
     7741 gaacaataac gatctctttc aggcatcacg tcggcgtttt ctggcacaac tcggcggctt
     7801 aaccgtcgcc gggatgctgg ggccgtcatt gttaacgccg cgccgtgcaa ctgcggcgca
     7861 agcggcgact gaggctgtca tctcgaaaga gggcattctt accgggtcgc actggggggc
     7921 tatccgcgcg atggtgaagg atggtcgctt tgtggcggca aaaccgttcg aactggataa
     7981 atatccgtcg aaaatgatcg ccggattgcc ggatcacgta cacaacgcgg cacgtattcg
     8041 ttatccgatg gtacgcgtgg actggctgcg taagcgccat ctgagcgaca cctcccagcg
     8101 cggtgataac cgttttgtgc gcgtgagctg ggatgaagcc ctcgacatgt tctatgaaga
     8161 actggaacgc gtacagaaaa ctcacgggcc gagtgccttg ctgaccgcca gtggttggca
     8221 atcgacgggg atgttccata acgcttcggg gatgctggcg aaagctattg ccttgcatgg
     8281 taatagcgtt ggtacgggcg gagattactc taccggtgct gcgcaggtga tcctgccgcg
     8341 cgtagtcggt tcaatggaag tgtatgaaca gcaaacctcc tggccgctgg tattgcagaa
     8401 cagcaaaacc attgtgctgt ggggctccga tttgctgaaa aaccagcaag cgaactggtg
     8461 gtgcccggat cacgatgttt atgaatatta cgagcagcta aaagcgaaag tcgccgccgg
     8521 tgaagttgag gtcatcagca tcgatccggt tgtcacatcc acccatgagt atctggggcg
     8581 cgagcatgtg aagcacattg cggttaaccc gcaaactgac gtgccgctgc aactggcgct
     8641 ggcacatacg ctgtacagtg aaaacctgta cgacaaaaac ttcctcgcta actactgtgt
     8701 gggttttgag cagttcctgc cgtatctgct gggtgagaaa gacggtcagc cgaaagatgc
     8761 cgcatgggct gaaaaactga ccggcattga tgccgaaacc attcgtgggc tggcgcggca
     8821 gatggcggcg aacagaacgc aaattattgc tggctggtgc gtgcagcgta tgcagcacgg
     8881 tgaacagtgg gcgtggatga ttgtggttct ggcggcgatg ctggggcaaa ttggcctgcc
     8941 aggtggtggt tttggctggc actacaacgg cgcaggcacg ccggggcgta aaggcgttat
     9001 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgactacaa
     9061 aggctacagc agcactattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
     9121 agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc
     9181 cggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa
     9241 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
     9301 cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
     9361 caaccgtggc attatcgcca tgaaacaggt ggtgccgccg tagttcgagg cgcgcaacga
     9421 ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgt gaagaagcct ttaccgaagg
     9481 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg
     9541 acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt
     9601 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
     9661 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta
     9721 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
     9781 cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
     9841 gcagctatgt gagtcggaaa cgctgcgtca gcaatatacg gtagcgggta aagagccagt
     9901 gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt
     9961 ctttaacgct cgcggtcagg tgttggcagg agcagtagtt tctgaccgct atgcacccgg
    10021 cgtggcgcga attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc
    10081 gctgtgcaaa tacggtaacc ccaacgtgct gaccatcgac atcgggactt cgcagctggc
    10141 gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaatg gaacagtgga
    10201 gcaggtgacg gcgtttaacg gccccgtgga gatggtggcg cagtgcgaat atgttcccgc
    10261 gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg
    10321 ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg
    10381 cagatggctg aatggttttc gttgctgaaa agcgaaccgc cgctcactgc ggcggtgaat
    10441 gatctggaaa acagtattgc cacg
//
LOCUS       DAZOYF010000139        10294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZOYF010000139 DAZOYF010000000
VERSION     DAZOYF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.140"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            312..401
                     /locus_tag="ACHUOF_003242"
                     /pseudo
     CDS             312..401
                     /locus_tag="ACHUOF_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            703..1698
                     /gene="pgl"
                     /locus_tag="ACHUOF_003243"
     CDS             703..1698
                     /gene="pgl"
                     /locus_tag="ACHUOF_003243"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR0612300.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1739..2692)
                     /locus_tag="ACHUOF_003244"
                     /pseudo
     CDS             complement(1739..2692)
                     /locus_tag="ACHUOF_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2876..3928
                     /locus_tag="ACHUOF_003245"
     CDS             2876..3928
                     /locus_tag="ACHUOF_003245"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFR0612301.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            4004..5437
                     /locus_tag="ACHUOF_003246"
     CDS             4004..5437
                     /locus_tag="ACHUOF_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFR0612302.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5620..7800
                     /locus_tag="ACHUOF_003247"
     CDS             5620..7800
                     /locus_tag="ACHUOF_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR0612303.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVEPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7941..9224)
                     /locus_tag="ACHUOF_003248"
     CDS             complement(7941..9224)
                     /locus_tag="ACHUOF_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR0612304.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9359..>10294)
                     /locus_tag="ACHUOF_003249"
     CDS             complement(9359..>10294)
                     /locus_tag="ACHUOF_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0612305.1"
                     /translation="TEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILAS
                     RGIKQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTL
                     SDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMEL
                     AVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCK
                     EILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQ
                     ISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2520 a         2390 c         2580 g         2804 t
ORIGIN      
        1 atgcagagga tttataccag aggacattag tcttgagtaa gatactacaa catctccaac
       61 tattttagcc agcctgacta cttgacttgg taatatttgt tgctaataat catgtataaa
      121 ataagcttat atacatagta acttcagcaa aggatttagt aatgatccaa atcatatctc
      181 aatgtactgt ttttttcttt aaaaaaacag tacattctat tttataatga ctgtgtattc
      241 ccatggaagg agatcaccca attctccccc ccgacttttg acgcagtgtc ttgttaacag
      301 ggggattgac catgcttatt ggctatgtac gcgtatcaac aaatgaccag aataccgatc
      361 tacaacgtaa tgcgtttatt tgtgccggaa gtgagttgat tgttgcgcga atgggaagtt
      421 ggccagaagt tgttatggca tagtgtttac ggtcgttgat acaggtaaac aaaagcgggg
      481 agagtcatac tccctgaata gtcgcgaaaa tatctgtggc attgtcaaat tcgtgtcggt
      541 gactaatatt ttagtaatat gccattcgaa atggagtgct aacttattga tttaattgat
      601 gtgcgatatt gtattttatc gtgttttgtt gtctgttttt tatccagtaa gtgttgttat
      661 atgtatgaaa gataagggat ttatcatttt tgaggtgtgt tcatgaagca aacagtttat
      721 atcgccagcc ctgagagcca gcaaattcac gtctggaacc tgaatcatga aggcgcactg
      781 acgctgacac aggttgtcga tgtgccgggg caggtgcagc cgatggtggt cagcccggac
      841 aaacgttatc tctatgttgg tgttcgccct gagtttcgcg tcctggcgta tcgtattgcc
      901 ccggacgatg gcgcactgac ctttgccgca gagtctgtgc tgccgggtag tccgacgcat
      961 atttccaccg atcaccaggg gcagtttgtc tttgtaggtt cttacaatgc gggtaacgtg
     1021 agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg tcgatgtagt cgaggggctg
     1081 gacggttgcc attccgccaa tatctcaccg gacaaccgta cgctgtgggt tccggcatta
     1141 aagcaggatc gcatttgcct gtttacggtc agcgatgatg gtcatctggt ggcgcaggac
     1201 cctgcggaag tgactaccgt tgaaggggcc ggcccgcgtc atatggtatt ccatccaaac
     1261 gaacaatatg cgtattgcgt caatgagtta aacagctcag tggatgtctg ggaactgaaa
     1321 gatccgcacg gcaatatcga atgtgtccag acgctggata tgatgccgga aaacttctcc
     1381 gacacccgtt gggcggctga tattcatatc accccggatg gtcgccattt atacgcctgc
     1441 gaccgtaccg ccagtctgat taccgttttc agcgtttcgg aagatggcag cgtgttgagt
     1501 aaagaaggct tccagccaac ggaaacccag ccgcgcggct tcaatgttga ttacagcggc
     1561 aagtatctga ttgccgctgg gcaaaaatct caccacatct cggtatacga aattgttggc
     1621 gagcaggggc tactgcatga aaaaggccgc tatgcggtcg ggcagggacc aatgtgggtg
     1681 gtggttaacg cacactaacc gctgatttaa ccggcgcagt ctctcctgcg ccggtgtatt
     1741 aacttatctc ctgtaacgca tgtctctggc gttcgacgat attggtccac aaattgtctt
     1801 tatcgtcagt ccacaaatta atcagcaagg aaaaaaagcg ttcagctgcc ggagaaagca
     1861 cggcatcttt gcggcgaata atccccaatg tccgacgtat aacgggttcc accagtggga
     1921 taccaataat cgaagaatag ggcgcatggg gcattgccag accaggaagc gccgaaatcc
     1981 ccagtcctgc ctccactagt cctaatgacg tcgaaagatg acgcacttcg taaaaccagt
     2041 ccagcttcca gggcttgtcg gctagctgtt gttctatcaa caatcggttg ccgctggagg
     2101 aacgtacgcc aatcattttg taacccacta actcctgcca ttctacaagc tactttttgg
     2161 ccaatggatg gtcacgccga caggccagca cgaatggctc gttgaccagt ggggtaaaat
     2221 caatggatga atttgtgacg ttgttcatgt ttatgccaaa gtctgattcg ttacacagca
     2281 ccgattccat gcaattattc gtgccttgtt ccagaatccg cactttaata ttgggataca
     2341 gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc ggaatacacg
     2401 ctaatgtcac cataccacga tgataagcat tcatatcacg aatattaaaa agcgtttcat
     2461 cgaatttctt tattaattcc cgcgcctccg ggagcaacct ttttcctgct tttgttaacg
     2521 taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc atctttttta
     2581 ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg ttaaatgaac
     2641 tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc attattactc
     2701 cggaaaatgg aagcgacgat tttggggggc tggtcgttaa aaattttaac tgcatttagc
     2761 caacttaaat taatgaaaaa atgttattaa tcgttgagct aaagtcatta gagatgcttt
     2821 gtccttaatg taaccatatc gcaataagtt atgtttttaa attgagggca ttattatgaa
     2881 aaaaataccc tgcgtgatga tgcgaggtgg aacctcgagg ggcgcgttcc tgttagcgga
     2941 acatttaccc gaagatcaaa cgcagcgcga taaaatattg atggcaatta tgggttccgg
     3001 taacgatctg gaaattgacg gtattggcgg cggtaatcca ctgaccagta aagtcgccat
     3061 tattagccgt tccagcgatc cgcgtgctga tgtcgattat ctgtttgctc aagttatcgt
     3121 ccatgagcaa cgtgtcgata ccacgcctaa ctgcggcaat atgctgtctg gcgtaggggc
     3181 attcgccatt gaaaatggtt tgattgcagc gacttcgcca gttacccgtg tacgtatccg
     3241 caacgtcaat acgggtacgt tcatcgaagc tgatgtgcaa acgccaaatg gtgttgtcga
     3301 gtacgagggt agcgccagaa ttgacggcgt accgggtact gccgcaccgg ttgcgctcac
     3361 tttcctgaat gccgctggaa cgaaaactgg aaaagttttc ccgactgata atcagattga
     3421 ttattttgac gatgtcccgg tgacctgtat cgatatggcg atgccagtcg tcattattcc
     3481 ggctgaatat ctgggaaaaa caggttatga attaccggcg gaactggatg ccgacaaagc
     3541 attattagcc cgcattgaat ctatccggct acaagcgggt aaagcaatgg gcttaggtga
     3601 tgtcagtaat atggttatcc ctaaacctgt gcttatttct ccagcgcaga aaggcggggc
     3661 aattaatgtg cgttatttta tgccgcattc ttgccatcgc gcgctggcga taaccggtgc
     3721 tattgctatt tccagtagtt gtgcattgga aggcaccgtc acccgacaaa tcgtcccttc
     3781 tgtaggatat ggcaatatca atattgaaca ccccagtggt gcgctcgacg ttcatttaag
     3841 taatgaaggt caggatgcca cgacgttacg cgcatctgtt attcggacaa ccagaaaaat
     3901 attttccggt gaagtttatc ttccctgaaa aaattcgttg tcaggataag gacaatcaat
     3961 aaaggacttc tgtatgagtc atacagaaag aacaggattt taaatgaata agaaatcgtt
     4021 atggaagcta attctgatat tagcgatccc atgtattatt ggttttatgc cagctccggc
     4081 aggattaagc gaactggcgt gggtgctttt tggtatttac ctggcggcca ttgtgggtct
     4141 ggttatcaag cctttcccgg aacctgtcgt actgttaatt gccgttgctg cctcaatggt
     4201 ggtggtcggt aacttatccg acggtgcgtt taagaccacc gccgtattaa gcggttactc
     4261 ttcaggtact acctggctgg tgttctcggc gtttacctta agcgccgcat ttgtgaccac
     4321 cggtttaggt aaacttattg cctatctgct gattggtaaa atcggtaaca ccatgctggg
     4381 tctgggttac gttacggtat tcctcgatct ggtactggct ccggcaacac cgtctaacac
     4441 cgcgcgtgcg ggcggcattg tgttaccgat catcaacagc gtggcggtgg ctttggggtc
     4501 cgaaccggaa aaaagtccgc gtcgtgtcgg acattacctg atgatgtcca tttacatggt
     4561 caccaaaacc accagctata tgttctttac cgcaatggcg gggaacattc tggcgctgaa
     4621 aatgatcaac gacattctgc acctgcaaat tagctggggt ggatgggcgc tggccgccgg
     4681 attgccgggc atcattatgc tgctggtcac cccgctggta atttacacca tgtatccacc
     4741 agaaattaaa aaggtggata acaaaaccat cgctaaagcg ggccttgccg aactgggacc
     4801 gatgaaaatc cgcgaaaaaa tgctgctcgg tgtctttgtg ctggcgctac tgggctggat
     4861 tttcagtaag tctctggggg ttgatgaatc cactgtggca atcgttgtta tggcgaccat
     4921 gctgctgctg ggtatcgtta cctgggaaga cgtggttaaa aataaaggcg gctggaatac
     4981 cttaatctgg tacggcggta ttatcggctt aagctcctta ttatcgaaag ttaaattttt
     5041 cgaatggtta gctgaagtct ttaaaaataa cctggcattt gatggtcacg gtaacgttgc
     5101 tttcttcgtt attattttcc tcagcatcat cgtgcgttat ttcttcgctt ccggtagtgc
     5161 ctatatcgtt gctatgttac ctgtatttgc catgctggcg aacgtctccg gcgcaccgtt
     5221 aatgttaacc gcgctggcac tgttgttctc caactcctat ggcggcatgg ttactcacta
     5281 tggcggcgcg gcaggtccgg tcatctttgg cgtgggttac aacgatatta aatcctggtg
     5341 gttggtcggt gcggtactga cgatattaac cttcttggtg catatcaccc tcggcgtgtg
     5401 gtggtggaat atgctgatcg gctggaacat gctgtaaata tactcgtcat acttcaagtt
     5461 gcatgtgctg cgtctgcgtt cgctcacccc agtcacttac ttatgtaagc tcctggggat
     5521 tcactcactt gtcgccttcc tgcaactcga attatttaga gtatatccat ttattatctt
     5581 tctgcgcact tcacggtgcg cagatatctg gagcatttga tgatcaagtt atctgaaaaa
     5641 ggcgtgtttc tcgccagtaa taacgaaata attgccgaag aacatttcac cggcgaaatt
     5701 aaaaaagaag aagcaaaaaa aggcactatt gcctggtcta ttctctcttc tcataatacg
     5761 tccggaaata tggataaact taaaattaag tttgattcat tagcctctca cgatattacc
     5821 tttgttggta ttgtacagac cgctaaagcg tccggaatgg aacgtttccc gctgccgtat
     5881 gtgctgacca actgccataa ctcactctgc gccgtcggcg gcaccattaa cggtgatgac
     5941 catgtttttg gtttatcggc agctcagcgt tatggcggta tttttgtgcc tccgcatatt
     6001 gcggtcatcc atcaatatat gcgtgagatg atggctggcg ggggcaaaat gatcctcggg
     6061 tcagacagcc acacccgtta cggtgcatta gggacaatgg cagtcggtga gggtggcggt
     6121 gagttggtaa aacagctgct taatgacacc tgggatatcg actatccggg cgtggttgcg
     6181 gtgcatctga ccggaaaacc tgcgccgtat gtggagccac aggatgtggc gctggctatc
     6241 attggcgcgg tgttcaaaaa cggttacgtc aaaaacaaag tcatggagtt cgttggaccg
     6301 ggcgttagcg cgctctctac cgatttccgt aacagcgttg acgtgatgac cacggaaacc
     6361 acctgtttaa gctccgtctg gcaaactgat gaagaagtgc ataactggct ggcgctgcac
     6421 ggtcgcggtc aggattactg ccagcttaac cctcaaccga tggcgtacta cgatggctgc
     6481 atcagcgttg atttaagcgc catcaaacca atgattgcgc tgccgttcca cccgagcaac
     6541 gtgtatgaaa tcgacacact gaaccagaac ctgaccgaca ttctgcgtga gattgaaatt
     6601 gagtccgaac gcgtggcgca cggtaaagcc aaactctcgc tgctggataa agtggaaaat
     6661 ggtcgcctga aagtgcagca ggggattatc gcgggctgtt ctggcggtaa ctacgaaaac
     6721 gtcatcgcgg cggcgaatgc actgcgcggt caatcctgtg gcaatgacac cttctcgctg
     6781 gcggtttacc cgtcatcaca gccggtgttt atggatctcg ccaaaaaagg tgtggtagca
     6841 gatttgattg gcgcaggcgc aatcatcaga accgcattct gtggcccatg ctttggcgcg
     6901 ggcgatacgc caatcaacaa cggtttgagt attcgccaca ccacgcgtaa cttcccgaac
     6961 cgcgaaggct ctaagccagc caatgggcag atgtcagcgg tggcgttgat ggacgctcgt
     7021 tctatcgctg cgactgcggc aaacggtggc tatttaacct ctgccagcga acttgattgc
     7081 tgggacaacg tgccggagta cgccttcgat gtaacgccgt ataaaaaccg tgtttatcag
     7141 ggctttgtga aaggggcaac tcagcaaccg ctgatttacg gaccgaacat taaagactgg
     7201 ccggaattgg gtgcgctgac tgacaatatc gtcctgaaag tgtgctcgaa gatcctcgac
     7261 gaagtcacca ccaccgacga actgattcct tccggtgaaa cctcttctta tcgttcaaat
     7321 ccgattggtc tggcggggaa tgtcagcgag ctgacagagg tgtttgcgcg cattaagcag
     7381 attgctggtc aggagcatat tgatccgctg caaactgaaa ttggtagcat ggtctatgcg
     7441 gtgaaaccag gcgatggttc tgcgcgtgaa caggcggcga gctgccagcg tgtgattggc
     7501 ggtctggcga atattgccga ggagtacgcg actaaacgct accgttctaa cgtcatcaac
     7561 tgggggatgt taccgctgca gatggcggaa gtgccaacct ttgaagtggg ggattacatt
     7621 tacatccctg gcattaaagc ggcgctggat aatccgggta cgacgtttaa aggttatgtg
     7681 atccatgaag atgcgccggt aacggaaatt acgctttaca tggaaagcct gacggctgaa
     7741 gagcgcgaga ttatcaaggc gggtagtttg attaacttca ataaaaaccg tcagatgtaa
     7801 aaagcgccat gtgaatgtag gtcgcattcg gcacttattg tcggatgcga tgctggcgca
     7861 tcttatccga cctacaaatc gcatcgaacc gtaggccgga taaggcgttt acgccgcatc
     7921 cggcaaatag ttaatagctc ttacttcttc tcctctgcaa ccactttgct gcccacgccg
     7981 cggttattgt attcccacat gcggttgtag ttagtgtcat tcagattgcg ctgtatttcg
     8041 tcgttatcat ctacgctgcc ggtattaccc gcaaacggac gattagagat caccgcatcg
     8101 gcccacggtt tagccgtgtt aaaaccttcg ttgatggcgc tatcacggat caccacctga
     8161 ccgttggtat tagcatcaac atccagcgag cggcccagtt gcgccacgcc atcaccggaa
     8221 gcattgaaac ggctgtttac ggcgaggaaa ccgtaataga tgttagacag cgtagccggt
     8281 gcaaacacat atgcttcttg ctgggtacgt gagttcacca cgcggaattc ggtgttatcg
     8341 aacaccactg cgccgcgacc agaaacgata tccacatccc cttcaatata gctgttggtc
     8401 accagcgtac gcggctgacg gttggtttcc agacggttct gcacaccgct gttggtgaca
     8461 aagaaggtgt tctgacgacc gagaatgtta acgttgttaa tctgtacttg gtcaccgtca
     8521 gtacgcagtg ctaccgccgg atggttacct gcatctacgc tatcgcccag cgtgttttcg
     8581 atggtcagat tttgcagttg cagtccattg ttttgtgacc agaagaccgc agaacacaga
     8641 acaccgatac tgtcgctgcg tttgctctgg cagctatcgt acatatacca cgctggttta
     8701 cctggcatat atttgccgcg cgggttgacg tcgtgacgcc agtcggcagg gctcatgccc
     8761 ccatcaaggg aaagcccaat cttcacatca atcggttttt cacctgtacc gtacagagtg
     8821 attccacctg gagcagcagg gacatacacc gttccctgat actcaccagg catcacggca
     8881 atatactggc gcttgttggt gcgcttgata attgccgcat ctaccgccgc ctgaatcgtg
     8941 gtatgcgtta caccttgagt acccgccggg ccgacaacaa agtcaggttg cgcaggcagg
     9001 gttatcgggg acggattcca cgctgcagca cctggtgtca gggatgcaaa atagtgttgc
     9061 gcatcgaaat tcagcgcttc ttttgccgac agaatcgggc gagaagaggt accaggcgca
     9121 gtttgatcag aaggacgttg atcgggtggt gttgagctac aggcggtcag cgtcacgcca
     9181 aaagccaatg ccagcgccag acgggaaact gaaaatgtgt tcacaggttg ctccgggcta
     9241 tgaaatagaa aaatgaatcc gttgaagcct gcttttttat actaagttgg cattataaaa
     9301 aagcattgct tatcaatttg ttgcaacgaa caggtcatta tcagtcaaaa taaaatcatt
     9361 atttgatttc aattttgtcc cactccctgc ctctgtcatc acgatactgt gatgccatgg
     9421 tgtccgactt gtgcccgaga agatgttgag caaacttatc gcttatctgc ttctcataga
     9481 gtcttgcaga caaactgcgc aactcgtgaa aggtaggtgg atccccttcg aaggaaagac
     9541 ctgatgcttt tcgtgcgcgc ataaaatacc ttgatactgt gccggatgaa agcggttcac
     9601 gacgagtaga tgcaattatg gtttctccgc caagaatctc tttgcattta tcaagtgttt
     9661 ccttcattga tatcccgaga gcatcaacat gcaatgttgt agggatggca atttttacgc
     9721 ctgttttgct ttgctcgaca taaagatatc catctacgat atcagaccac ttcatttcgc
     9781 ataaatcacc aactcgttgc ccggtaacaa cagccagttc cattgcaagt ctgagccaac
     9841 atggtgatga ttctgctgct tgataaattt tcaggtattc gtcagccgta agtcttgatc
     9901 tccttacctc tgattttgct gcgcgagtgg cagcgaccgg gtttgttgtt atatggcctt
     9961 cagctattgc ctctcggaat gcatcgctca gtgttgatct gattaacttg gctgatgccg
    10021 ccttgccctc gtctatgtat ccattgagca ttgccgcaat ttcttttgtg gtgatgtctt
    10081 caagtggagc atcaggcagc cccctcctta ttgctttaat tttgctcatg taatttatga
    10141 gtgtcttctg cttgattcct ctgctggcga ggattttttc gtagcgatca agccatgaat
    10201 gtaacgtaac agaattatca ctgttgattc tcgctgtcag aggcttgtgt ttgtgtcctg
    10261 aaaataactc aatgttggcc tgtatagctt cagt
//
LOCUS       DAZOYF010000140        10184 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZOYF010000140 DAZOYF010000000
VERSION     DAZOYF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.141"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            423..1289
                     /gene="folD"
                     /locus_tag="ACHUOF_003250"
     CDS             423..1289
                     /gene="folD"
                     /locus_tag="ACHUOF_003250"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR0612306.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1291..1503
                     /gene="ybcJ"
                     /locus_tag="ACHUOF_003251"
     CDS             1291..1503
                     /gene="ybcJ"
                     /locus_tag="ACHUOF_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR0612307.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1611..2120
                     /locus_tag="ACHUOF_003252"
     CDS             1611..2120
                     /locus_tag="ACHUOF_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0612308.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVLAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(2156..3541)
                     /gene="cysS"
                     /locus_tag="ACHUOF_003253"
     CDS             complement(2156..3541)
                     /gene="cysS"
                     /locus_tag="ACHUOF_003253"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR0612309.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3715..4209
                     /gene="ppiB"
                     /locus_tag="ACHUOF_003254"
     CDS             3715..4209
                     /gene="ppiB"
                     /locus_tag="ACHUOF_003254"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR0612310.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            4212..4934
                     /gene="lpxH"
                     /locus_tag="ACHUOF_003255"
     CDS             4212..4934
                     /gene="lpxH"
                     /locus_tag="ACHUOF_003255"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR0612311.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            5052..5561
                     /gene="purE"
                     /locus_tag="ACHUOF_003256"
     CDS             5052..5561
                     /gene="purE"
                     /locus_tag="ACHUOF_003256"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR0612312.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            5558..6625
                     /gene="purK"
                     /locus_tag="ACHUOF_003257"
     CDS             5558..6625
                     /gene="purK"
                     /locus_tag="ACHUOF_003257"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR0612313.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6762..7655)
                     /locus_tag="ACHUOF_003258"
     CDS             complement(6762..7655)
                     /locus_tag="ACHUOF_003258"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR0612314.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7652..8299)
                     /locus_tag="ACHUOF_003259"
                     /pseudo
     CDS             complement(7652..8299)
                     /locus_tag="ACHUOF_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            8302..9534
                     /gene="allC"
                     /locus_tag="ACHUOF_003260"
     CDS             8302..9534
                     /gene="allC"
                     /locus_tag="ACHUOF_003260"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFR0612315.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            9545..>10184
                     /gene="allE"
                     /locus_tag="ACHUOF_003261"
     CDS             9545..>10184
                     /gene="allE"
                     /locus_tag="ACHUOF_003261"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFR0612316.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2372 a         2728 c         2689 g         2395 t
ORIGIN      
        1 ttaagacact gatagtagta aaattccttt ttatcctcta agaatgtctt aattgaaaat
       61 atgcactcta ttctaaaaaa tagagagccc cgttagatga atacttccgc gcaaaatata
      121 ttcaccacaa atatagacct gaagcggtaa attaccaggc tgaaaattct ttttatattg
      181 tcaggtattt cttaaattat cttaatcctt aaacaaggaa ataaatcagt tccagattta
      241 caacgccatc atgtacgaaa aatgaagctt tcaatctcag cgacctcagc gacggtgcgc
      301 ctaaccttcg caagaggtcg cttcacgcga taaatctgaa acgaaacctg acagcgcgcc
      361 ccgcttctga caaaatagac gcatcccctt cgacctacgt aacagatgga atcctctctc
      421 tgatggcagc aaagattatt gacggtaaaa cgattgcgca gcaggtgcgc tctgaagttg
      481 ctcaaaaagt tcaggctcgt attgcagccg gactgcgggc accaggactg gccgttgtgc
      541 tggtgggtag taaccctgca tcgcaaattt atgtcgcaag caaacgcaag gcttgtgaag
      601 aagtcgggtt cgtctcccgc tcttatgacc tcccggaaac caccagcgaa gcggagctgc
      661 tggagcttat cgatgcgctg aatgccgaca acaccatcga tggcattctg gttcaactgc
      721 cgttaccggc gggtattgat aacgtcaaag tgctggaacg tattcatccg gacaaagacg
      781 tggacggttt ccatccttac aacgtcggtc gtctgtgcca gcgcgcgccg cgtctgcgtc
      841 cctgcacccc gcgcggtatc gtcacgctgc ttgagcgtta caacattgat accttcggcc
      901 tcaacgccgt ggtgattggc gcatcgaata tcgtcggtcg cccaatgagc atggaactgt
      961 tgctcgcagg ttgcaccacc accgtgactc accgcttcac taaaaatctg cgtcatcacg
     1021 tagaaaatgc cgatctgttg atcgttgccg ttggcaagcc aggctttatt cccggtgact
     1081 ggatcaaaga aggcgcaatt gtgattgatg tcggcatcaa ccgtctggaa aatggcaaag
     1141 ttgtgggcga cgtcgtgttt gaagacgcgg ctaaacgcgc ctcatacatt acgcctgttc
     1201 ccggcggcgt tggcccgatg acggttgcca cgctgattga aaacacgcta caggcgtgcg
     1261 ttgaatatca tgatccacag ggtgagtaac atggcgacat tttctttagg taaacatccg
     1321 cacgttgagc tgtgcgactt gctgaaactg gaaggctgga gcgaaagcgg cgcgcaggcg
     1381 aaaatcgcga ttgccgaagg ccaggtgaaa gtcgacggtg cggttgaaac gcgcaaacgc
     1441 tgcaaaatcg tcgccggtca gacagtgagt tttgcaggac acagcgtaca ggttgttgcc
     1501 tgatcccttt cccggcctgt atcaacaggc cggttctctt ttcagacttt tgccctctac
     1561 gccccgttca tccatgctga tagcatcgac attttgccga agcgaccatc atgccaaccg
     1621 ttattacgca cgccgccgtt cccctttgca ttggtttagg gttgggttcg aaaggtctgt
     1681 tatttgccgg aatcatcctg gcgatgctgc ccgatgccga cgtattgtcg tttaaatttg
     1741 gcgttgctta cggcaatgtt tttggtcatc gcggttttac ccattcgctg gtgcttgcgt
     1801 ttgttgtccc gctcttatgt gtgctgattg gacgtcgatg gttcagggct gggctgattc
     1861 gctgctggct gtttttaacc gtctcgttgc tatcgcacag cttactggat tcggtaacca
     1921 ctggcggtaa gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt ttcgctccct
     1981 ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta caccacagcg tacgggcatc
     2041 aggtgattat ttccgaattg atgtgggtgt ggttgccggg gatgctactg atgggaatgt
     2101 tgtggtggcg cagacgataa ccggatgcga aaactcgcat ccggcaattg cgcaattact
     2161 tacgacgcca ggtggtccct tgcgggccat cttccagcac gatccccatc tcgttaagac
     2221 ggtcacgtgc cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc agacgctgtt
     2281 gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg ctttgcagga
     2341 acgcttccgg ttcttgctcc agcaggccca atacagcgga aagtttacgc aggtgagatg
     2401 ccattgcatt cgccgctgcc atatcttctg ctttcagacg gttcacttca cgcgccatat
     2461 caaacagcac ggaataggct tccggggtgt tgaaatcgtc gtccatcgct tcaataaagc
     2521 gcgcttcaaa cgcttcgcca ccggcaggcg caacggtttt atctgtgccg cgcagcgcag
     2581 tgtagaggcg ctccagcgcc gcacgcgcct gcttcaggtt ctcttcgctg tagttcagct
     2641 ggctgcgata gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg tagtatttca
     2701 gcacatcgcg cacggtaaag aagttaccca gcgatttgga catcttctcg cggtcaacca
     2761 tcaccattcc ggagtgcatc cagtagttca catactgacc atcatgggca caggtggact
     2821 gcgcgatttc gttctcgtgg tgcgggaaca tcaggtctga accgccgccg tggatatcaa
     2881 agtggttacc cagctgcttg cagttcatgg ccgaacattc aatgtgccag ccaggacgac
     2941 ccgcgcccca cggagacggc cagctcggtt cgccctcttt cgacatcttc cacagaacga
     3001 agtccatccg gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc tgcagctggt
     3061 cgagatcctg acgcgacagc acgccataag ttggatcggt cgggacgtcg aacatcacgt
     3121 cgctgttgtc cgccacataa gcgtgacctt tggcgatcag ttgttcagtg agttcaataa
     3181 tttctgcgat atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg ttcaaagcat
     3241 caaaatcttt gtgcatttcg gcgatcatgc gatccaccag cgccacaaag ctttcgccat
     3301 tttcattggc gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc acatacttca
     3361 gtttatagcc gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca aaggtacgcc
     3421 cgtgaccgat atgacagaga tcgtaaacgg tgattccaca cacgtacatg ccgacttccc
     3481 cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag agtattgaag atttttagca
     3541 tcgaagattc cgtttagaca tgtgtgggta attgagttgc gtataatacc catatttccc
     3601 gcgcgaatca gcatacattg cgtgatgatc cgatcgtgcg gttatgctat aacaccaccc
     3661 tatatatgac ccgaactggg ttgaagcacc aatcaaacgg aacaggatgc aaaaatggtt
     3721 actttccaca ccaatcacgg cgatattgtc atcaaaactt ttgacgataa agcacctgaa
     3781 acagttaaaa acttcctgga ctactgccgc gaaggttttt acaacaacac cattttccac
     3841 cgtgttatca acggctttat gattcagggc ggcggttttg aaccgggcat gaaacaaaaa
     3901 gccaccaaag aaccgatcaa aaacgaagcc aacaacggcc tgaaaaatac ccgtggtacg
     3961 ctggcaatgg cacgtactca ggctccgcac tctgcaactg cacagttctt catcaacgtg
     4021 gttgataacg acttcctgaa cttctctggc gaaagcctgc aaggttgggg ctactgcgtg
     4081 tttgctgaag tggttgacgg catggacgtg gtagacaaaa tcaaaggtgt tgcaaccggt
     4141 cgtaacggta tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag cgtgaccgtt
     4201 agcgagtaat cgtggcgaca ctctttattg cagatcttca tctctgcgtg gaagaaccgg
     4261 cgatcaccgc cggttttctg cgttttttag cgggggaagc ccgcaaggcc gacgcgctgt
     4321 atattcttgg cgatctgttt gaagcatgga ttggcgacga cgatcccaac ccactccatc
     4381 gccagatggc ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt tatttcattc
     4441 atggcaaccg tgattttctg ctcggcaaac gctttgcccg tgaaagcggc atgacattat
     4501 tgccggaaga aaaggtgctc gaactttatg gtcgccgggt gttgattatg catggcgaca
     4561 cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc caaagtccac aaaccctggc
     4621 tgcaaatgct attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc gcgcgaatgc
     4681 gcgcgaacag caaagaagcc aacagcagta aatcgctggc gatcatggac gttaaccaaa
     4741 acgctgtggt cagtgcgatg gaaaaacatc aggtgcaatg gctaatccac gggcataccc
     4801 atcgcccggc ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc gtggtactgg
     4861 gtgcctggca tacggaaggt tcgatggtga aagtcacggc tgatgacgtt gagctgattc
     4921 attttccgtt ttaaaaaacc cacaactttg ctgatttcac agccacgcaa ccgttttcct
     4981 tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc accacaggag
     5041 ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg atggggtcca
     5101 aaagcgactg ggctaccatg cagttcgccg tcgaaatctt cgaaatcctg aatgtcccgc
     5161 accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc ttcgccgaaa
     5221 gcgccgaaga gaacggttat caggtgatta ttgcgggcgc aggcggcgca gcgcacctgc
     5281 caggcatgat tgccgccaaa acgctggtgc cggtgctggg cgtgccagta cagagcgccg
     5341 cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc attccggtgg
     5401 gtacgctggc gattggtaaa gctggcgcgg caaacgcggc attactggca gcacaaattc
     5461 tcgcgactca cgataaagag ctacaccagc gtctgaatgg ctggcgcaaa gcccagaccg
     5521 acgaagtgct ggaaaacccg gacccgcgag gtgcggcatg aaacaggttt gcgtcctcgg
     5581 taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca ttgctgtctg
     5641 gccggtcggg ctggacgctg aaccggcggc ggtgcctttt caacaaagcg tgattaccgc
     5701 tgagatcgaa cgctggccgg aaaccgcatt aacccgcgag ctggcgcgtc atccggcctt
     5761 tgtgaaccgc gatgtgttcc cgattattgc tgaccgtctg actcagaaac agcttttcga
     5821 taagctccac ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg agtggcctgc
     5881 ggtgtttgat cgtttaggtg aactggcgat tgttaagcgt cgcactggtg gctatgacgg
     5941 tcgcggtcaa tggagtttac gcgccaatga aaccgaacag ttaccggcag agtgttacgg
     6001 tgaatgtatt gtcgagcagg gcattaactt ctctggtgaa gtatcgctgg ttggcgcgcg
     6061 cggctttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc aggacggtat
     6121 tttgcgcacc agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc aagccgaaga
     6181 gatgctgtcg gcgattatgc aggagctggg ctatgtgggc gtgatggcga tggagtgttt
     6241 tgtcaccccg caaggtctgc tgatcaacga actggctccg cgtgtgcata acagcggtca
     6301 ctggacacaa aacggtgcca gcatcagcca gtttgagctg catctgcggg cgattaccga
     6361 tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc tgattggtag
     6421 cgatgtgaat tatgactggc tgaaattgcc gctggtgcat ctgcactggt acgacaaaga
     6481 agtccgtccg gggcgtaaag tggggcatct gaatttgacc gacagcgaca catcgcgtct
     6541 gactgcgacg ctggaagcct taatcccgct gctgccgcca gagtatgcca gcggcgtgat
     6601 ttgggcgcag agtaagttca gttaagcagg tgctcatttg attgccggat gcggcgtaaa
     6661 cgccttatcc ggcttaccgc tccggtaccc attgtaggcc tgataagacg cgtcaagcgt
     6721 cgcatcaggc atggtgcacc gattgccgga tgctctcatc actacagcga aatacaggtc
     6781 cccgcttcgc ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca cgcgggttta
     6841 ctacggcttc tgacataacc actcaccgcc gttaccttcg gccccatcga accatcggct
     6901 ttggcaaatg gcgctaactc atccggtgtg gcatggcgaa tggcacgttg ctgcggcgtt
     6961 ccccagtttt catataccgc atcagcatcg gtgaggatca ccagtccatc ggcattaatc
     7021 tgctcggcga gcaacgcagc ggcgagatct ttatcaatca ccgcttcact ccctgcccca
     7081 tcttccgtca caggcacacc gccgccgcca ctgcaaatca ccacatgccc ctctttgagc
     7141 aacaactcga tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc caccacccgg
     7201 cgcaaatatt taccatcacg tttcatctgc cagccgtaag ccgcttccag tgcctcttgt
     7261 tcttctggct gataaaccgg accaataaat ttctctggct gcaaaaacgc cggatcatca
     7321 ggcgaaacct caatgcgcgt cagcaccgtc gtaacaggcg gcatctgcgg ctgtgcgctc
     7381 aagctctgcg ccagcatata gccaatcatc ccctggcttt ccgccaccag tacatccagc
     7441 ggatacggtt ctacctcttt ccacgccaga ttctgtaacg ccagcagccc cacctgcggc
     7501 ccgttgccgt gaacaatcgc cagccgataa gaacgggcca ggcgtgccag cgcgggtaca
     7561 gcactggcga tattgcgata ttgattttcc gccgtcagcg cctcaccgcg ctggagtaag
     7621 gcgttgcccc caagagctac aaccagtgtt ttcatggttt tccttgtaat aattgttggc
     7681 caagccagaa ccccagcagc gtatctgccc ctgacgtatg ccccagcgcc agcagcgaat
     7741 caatcgcaac agcggtacgt ttcgggcaac tcagagcatg gacaaagtgc aggagtggcg
     7801 aggcgaaata tccttgcgcg gcataacgta aataactgac gctcaccgct gtggtaacga
     7861 gttgaagatt gtcggaacag gcaaaaaacg ggcgaccggc gcgcgcatct aaagcgccat
     7921 aataccaggc cgccagcagc attccgctca acgtgtcatc atgacttggc gttaatccgg
     7981 ggcctttacc cagccagtgc cgccagtcgg tcttaacgcc attgagcgcg gcctgaaaac
     8041 agtgacgaaa ctggcgtaat tcagcgggca gcggatcgct tgccgccatc gccagtggcc
     8101 cgaaaagtcc ggtttcctcc gcgcgttgca tccatgcagc tgcaagcggt tgaggatgcg
     8161 aaggcggcgt aatacgcagc aaacaataac gctgtggctg tttaaccgtg aaacgcccga
     8221 ggcgaatccc ttgagccaca acctgtggtc gttcattgcc gcataatcca ccgcataacg
     8281 catcgaattg cgtgcggcga aattacacat tcccgtcaag ctatagaaga aacgctgccc
     8341 tggctttcct cttttggcgc tgacccaacg ggtgggatga cccgtttact ttattcgccg
     8401 gaatggctgg aaacccagca gcaatttaaa aaaagaatgg cagcaagtgg gctggaaaca
     8461 cgtttcgatg aagtggggaa tttatacggt cgcctgagtg gcaccgaata tccacaggaa
     8521 gtggttctga gcggttcgca tatcgatacc gtggttaacg gcggtaacct cgacgggcaa
     8581 ttcggcgcgc tggcggcgtg gctggcaatt gactggttga aaacgcaata cggcgcaccg
     8641 ttacgtacgg tcgaagtggt ggcgatggca gaagaagaag gcagccgctt cccgtatgtc
     8701 ttctggggca gtaaaaatat ctttgggctg gcgaatcctg acgacgtgcg gaatatctgt
     8761 gatgccaaag gaaatagttt tgccgatgcg atgaaggctt gcggatttac tctgccgaac
     8821 gccccactaa ctccgcgtca ggctataaaa gcctttgttg aactgcatat tgaacagggc
     8881 tgtgtgctgg aaagtaatgg gcaatcaatt ggcgtggtga atgcaattgt cgggcagcgt
     8941 cgctatacgg tgacgctgaa cggcgaatca aaccatgcag gcaccacgcc gatgggttat
     9001 cgtcgtgata cggtttacgc tttcagtcgc atttgccatc agtcgatcga aaaagcgaaa
     9061 aagatgggcg atccgctggt tctgaccttt ggcaaagtag agccgcgccc gaatacggta
     9121 aatgtggtgc cgggtaaaac cacgttcacc attgattgtc gtcataccga cgctgccgtg
     9181 ctgcgcgatt tcacccaaca gttagaaaac gacatgcggg cgatttgcga tgaaatggac
     9241 attggtattg atatcgattt atggatggac gaagaacccg tgccgatgaa taaggagctg
     9301 gtcgccaccc tgacagaatt gtgtgaaaga gaaaaactga attaccgggt gatgcacagt
     9361 ggtgccgggc acgacgcgca aattttcgcg cctcgcgtac caacctgcat gatttttatc
     9421 cccagcatca acgggatcag ccataacccg gcggaacgca ccaatattac tgaccttgcc
     9481 gaaggggtca aaacgttggc actcatgctt tatcaacttg cctggcagga ataaggagtc
     9541 ataaatggga tatttaaata acgtcaccgg ttaccgcgaa gatttactgg ctaaccgtgc
     9601 gattgttaaa cacggtaatt tcgcactgtt aaccccggat ggtctgataa aaaatattat
     9661 tccggacttt gaaaattgtg acgcgacaat cctctccacg ccaaagctgg gtgcctcttt
     9721 tgttgattat ctggtcacac tgcatcaaaa cggtggcaac caacagggct tcggtggcga
     9781 aggcattgaa acgttcctgt atgtgatctc tggaaatatc actgccaaag ccgaaggcaa
     9841 aacatttgcc ttaagcgaag gtggctatct ttattgccca ccaggctcct taatgacgtt
     9901 tgttaacgcc caggccgaag acagccaaat ctttttatat aagcgccgct atgttccggt
     9961 agaaggccat gcaccgtggc tgttttctgg caatgccagc gaactggaac gcattcacta
    10021 tgaaggtatg gatgatgtga tcctgctgga ttttctgccc aaagagttag gttttgatat
    10081 gaacatgcat atcctctctt ttgcaccagg tgccagccac ggttatatcg aaacacacgt
    10141 tcaggaacat ggtgcctata ttctttccgg tcagggggtt tata
//
LOCUS       DAZOYF010000141        10032 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZOYF010000141 DAZOYF010000000
VERSION     DAZOYF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.142"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(1..406)
                     /locus_tag="ACHUOF_003262"
                     /pseudo
     CDS             complement(1..406)
                     /locus_tag="ACHUOF_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            507..1106
                     /locus_tag="ACHUOF_003263"
     CDS             507..1106
                     /locus_tag="ACHUOF_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR0612317.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            1214..1981
                     /gene="tpiA"
                     /locus_tag="ACHUOF_003264"
     CDS             1214..1981
                     /gene="tpiA"
                     /locus_tag="ACHUOF_003264"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR0612318.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(2036..2791)
                     /gene="cdh"
                     /locus_tag="ACHUOF_003265"
     CDS             complement(2036..2791)
                     /gene="cdh"
                     /locus_tag="ACHUOF_003265"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR0612319.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(2898..3887)
                     /gene="sbp"
                     /locus_tag="ACHUOF_003266"
     CDS             complement(2898..3887)
                     /gene="sbp"
                     /locus_tag="ACHUOF_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR0612320.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(4207..5169)
                     /gene="pfkA"
                     /locus_tag="ACHUOF_003267"
     CDS             complement(4207..5169)
                     /gene="pfkA"
                     /locus_tag="ACHUOF_003267"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR0612321.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(5350..6252)
                     /gene="fieF"
                     /locus_tag="ACHUOF_003268"
     CDS             complement(5350..6252)
                     /gene="fieF"
                     /locus_tag="ACHUOF_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR0612322.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(6400..6900)
                     /gene="cpxP"
                     /locus_tag="ACHUOF_003269"
     CDS             complement(6400..6900)
                     /gene="cpxP"
                     /locus_tag="ACHUOF_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR0612323.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7050..7748
                     /gene="cpxR"
                     /locus_tag="ACHUOF_003270"
     CDS             7050..7748
                     /gene="cpxR"
                     /locus_tag="ACHUOF_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR0612324.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            7745..9118
                     /gene="cpxA"
                     /locus_tag="ACHUOF_003271"
     CDS             7745..9118
                     /gene="cpxA"
                     /locus_tag="ACHUOF_003271"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR0612325.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9224..9898)
                     /gene="yiiM"
                     /locus_tag="ACHUOF_003272"
     CDS             complement(9224..9898)
                     /gene="yiiM"
                     /locus_tag="ACHUOF_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR0612326.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT         2525 a         2666 c         2452 g         2389 t
ORIGIN      
        1 catcctgagt atctttacca tatttatttt cgccttgtgt gccaacaaac gcaccaagat
       61 cgataagcat catcaccaga atcaacgtcg ggacaaaacg ccccaccgcc cattgccaga
      121 cacccggtaa aatcgctcag ttacccgcca gcagcatcca cgccacaatc atcagaaatg
      181 cccatgcgcc ggaacgcccg cgatcatgca agcgcttaac agttactgcc gctgttggcc
      241 agagcaagca cacaaggcaa aacgccgcgg tctgaatatc gagtagattc ttacccgcca
      301 gtgagaaaag caccagcatg cctgcgaacc acaggcctat ccaaatccag aaatcacggc
      361 gtccaatacg ccctttaaat gagaataacc attgctgtat ggtcatgtaa gttccttgat
      421 ggttgtcttt tccaggattc tacccttttg acaagggcga cagatatcgt tttaatcgga
      481 gccagtcata acaaaaggta ctgtcaatga agccagggtg tacgctgttt tttctcttat
      541 gttctgcatt aaccgttaca acaacggcgc atgcacaaac accagatacg gcaacgaccg
      601 cgccttatct gctggctgga gcccctactt tcgatctctc catcagccag tttcgagaag
      661 actttaacag ccagaatccc agcctgccac tgaacgaatt tcgtgccatc gacagcagtc
      721 ccgacaaagc caatctcact cgtgctgcaa gtaaaattaa cgagaacttg tatgcttcta
      781 cagcgctgga gcgcggtacc ttaaaaatca aaagcattca aatgacctgg ctacccatcc
      841 aggggccaga gcaaaaagca gcgaaagcga aagctcagga atacatggca gcagtgatcc
      901 gcacactcac cccattaatg accaaaacac aaagccagaa aaaactgcag tcgctactaa
      961 cggcggggaa aaacaaacgt tattacaccg agacagaagg tgcactgcgt tatgttgtcg
     1021 cggacaacgg cgaaaagggg ctgaccttcg ctgttgaacc gattaagctg gcgctatctg
     1081 aatcgcttga aggtttgaat aaatgacaaa aagcaaagcc tttgtgccga tgaatctcta
     1141 tactgtttca cagacctgct gccctgcggg gcggccatct tcctttattc gcttataagc
     1201 gtggagaatt aaaatgcgac atcctttagt gatgggtaac tggaaactga acggcagccg
     1261 ccacatggtt cacgagctgg tttctaacct gcgtaaagag ctggcaggtg ttgctggctg
     1321 tgcggttgca atcgcaccac cggaaatgta catcgatatg gcgaagcgcg aagctgaagg
     1381 cagccacatc atgctgggtg cgcaaaacgt ggacctgaac ctgtccggcg cattcaccgg
     1441 tgaaacttct gccgctatgc tgaaagacat cggcgctcag tacatcatca tcggccactc
     1501 tgaacgtcgt acttaccaca aagagtctga cgaactgatt gcgaaaaaat tcgcggtgct
     1561 gaaagagcag ggtctgactc cggttctgtg catcggtgaa accgaagctg aaaacgaagc
     1621 gggaaaaact gaagaagttt gcgcacgtca gatcgacgcg gtactgaaaa ctcagggtgc
     1681 tgcggcattc gaaggtgcgg ttatcgctta cgaacctgta tgggcaatcg gtactggcaa
     1741 atctgcaact ccggctcagg cacaggctgt tcacaaattc atccgtgacc acatcgctaa
     1801 agttgacgct aacatcgctg aacaagtgat cattcagtac ggcggctctg taaacgcgtc
     1861 taacgctgca gaactgtttg ctcagccaga catcgacggc gcgctggttg gcggtgcttc
     1921 tctgaaagct gacgctttcg cagtgatcgt taaagctgca gaagcggcta aacaggctta
     1981 agtctgacag gtgccggatt tcatatccgg cacttacttt ccttaactcc tcgccttaac
     2041 gcaaaatctc acactcatga tcctggattt cctcggctga agcacggttc agcgtcagta
     2101 gatttcgttg tgtcgccagc aacacaaacg aattatcact ctgccgtacc atcgccagcc
     2161 cgtagcttcc catatgttcc cgcgcctcag gcacttcttc tgcaagcatc ataaatgggc
     2221 tgcgttgtac cagttcgctt tccgttaccc gacgcgccag gtattcatgc ccgcgcaaac
     2281 cacctggcag tggcaaccag cggctgctga tgttcgccag atttttatcc agttgttcgc
     2341 gcacatcagg acgaatacaa gagatatgaa tatgaaaatg gttttgcgta tgcccggtgc
     2401 gggagttgat cgccaaagaa accgcgcgat cgggaaccgg ctggccgtat tttttgctca
     2461 taaaatcacg cgcctgccag gccaaccaaa agaagttcgg cgttgaagga tcggtcaaca
     2521 aaggactttc agtaccgtta atacgatacg ttggcatcaa cagatattgc agtggaccat
     2581 taagatcttt taaaaccacg tatccggcat tgggtttgac ttccgcacat ggcgaaggat
     2641 tttgattttg ctgctgattg ggcaaacatt cctcaaggac aatcttacgt aatgtatccg
     2701 actcttcacc ggttaatttc cagtaaccaa taccggcagc cacaacggcg ataactatca
     2761 tcaccaaaaa aagaagaccc gcttttttca tctttttttc cctgtacctc aaagagatgc
     2821 aagggtaacg caaaatcgtg acaaataaaa aacccggtcg cgagaaacca ccgggtttga
     2881 taattatcct agggaaatca gcgtttgctg atctgatcga acgtaccgcc gttagcaaaa
     2941 tgctcttttt gcgctttcgt ccagccgccg aactcttcat caatggtgaa taacttcagc
     3001 tttggaaacg cattttcgta ctttttcgct acttcagcgt cgcgcggacg gtagtagttt
     3061 ttcgcggcaa tttcctgacc ttctggcgag tagagatatt tcaggtaggc ttccgccacc
     3121 tctttggtgc cttttttctc gaccacttta tcgaccaccg acaccgttgg ctctgcgagg
     3181 atagactcac tcggcgtgac gatttcgaat ttatctttcc ccagttcatt cgctgccagc
     3241 agagcttcgt tttcccaggc gatcagtaca tcgccaatcc cgcgctcgac aaaggtgtta
     3301 gtggagccgc gcgcgccaga atccagaact tcgacgtttt tatacagtgc ccgaacaaaa
     3361 tcctgtgctt ttgcctgatc gttgttgttg tgatgcagcg cgtagcccca ggcagccagg
     3421 tagttccagc gtgcgccacc agagcttttc ggattaggcg tgatcaccga aacacccggt
     3481 ttaatcagat cgttccagtc atggatctgc ttcggattgc ccttacgcac caggaaaaca
     3541 atggtggaag tgtacggtgc ggagttatcc ggcagacgtt tgatccactc tttatcaatc
     3601 cgcccgcgtt ccgcaatcgc gtccacgtca taggccagag ccagcgtgac aacatcagct
     3661 tcaataccgt tgattaccga cgtcgcttgt ttacctgaac caccgtgtga ctgacgaatc
     3721 accacgttat caccagtttg ctgtttccag tgggcgctga atgccttgtt gtactgttcg
     3781 tacaattcgc gcgttggatc atatgaaacg ttaagaagct gaatatcctt tgccataacg
     3841 ctggttgccg ccagcaaaaa tgttaaccct acgccccact tgttcatcgc ccgactctct
     3901 tatgttgtgt tgtgatgagc aaagcgtgcc agaaggttaa ccaaacatta aagaataaaa
     3961 aaagattggc tataacttgc gggtatatgt tgagggatta aaaaggcgga agaaatccgc
     4021 ctcatattgc tgacaaagtg cgctttgtcc atgccggatg cggcgcgaac gccttatccg
     4081 gcctacaaaa gtttgcaaat tcaataaatt gcagaattca tgtaggcctg ataagcgaag
     4141 cgcatcaggc atttttgctt ctgtcatcgg tttcaggcta aaggaatctg cctttttccg
     4201 aaatcattaa tacagttttt tcgcgcagtc cagccagtca cctttgaacg gacgcttcat
     4261 gttttcgata gcgtcgatga tgtcgtggtg aaccagctgt tcgttctgga tacctacaca
     4321 acgaccgccg taacctgcca gcagcagatc gatagcgtaa gcgcccatac gggaagccag
     4381 aatacggtcg taaggcaccg gagaaccacc gcgctggatg tggcccagca cagttgcgcg
     4441 ggtttcacga ccggtttctt tctcgatgaa atgcgccagt tcgtcaacat cacacatatg
     4501 ttcggtaatc gccacgatcg cgtgtttttt acctttcgcg atacccgctt tgatttcgtt
     4561 taccaggtct tcacggctga attcaacttc cggaactaca acgaattcac agccaccggc
     4621 aatggccgca gccagggtca gatcgccaca ataacggccc atcacttcca ccacggaaat
     4681 acgctggtga gaagaagagg tgtcacgcag acggtcgatc gcttctacaa cggtgctcag
     4741 cgcagtgaag aaaccgatag tgtagtcagt gcctttgatg tcgttgtcga tagtgcccgg
     4801 cagaccgatg cacgggaagc ccatttcggt cagacgcatt gcacccatgt aggaaccgtc
     4861 accgccgata accaccagcg cgtcgatacc acgttttttc aggttttcga tagccacggc
     4921 gcggatgttc tcgtcgcgga attccgggaa acgcgcagaa ccgaggaacg taccgccacg
     4981 gttgatcatg tcagacacgc tgtaacggtc tagctgtacc atacggtctt catacagacc
     5041 cagatagccg tcataaatac ccattacttc cagaccttct gtcagcgcag aacgaacaac
     5101 cccgcgaatt gcggcgttca tgcctggcgc atcaccgccg cttgtcaaca cacctatttt
     5161 cttaatcatg actacctctg aactttggaa tgcaaaatga aatctgttgc cggaagtctt
     5221 cttgcacatc gaagtgatcc aacgaatgtg caaatagtat aacaatcact tcctgctgaa
     5281 ttgattcagg tcaggccaaa tggcggtatt ttatacacaa aatgcgggtc tggctctctt
     5341 ttatactgat tatgaaagca tagaccgttt accctccctg ggtacgacgg aacaggggtc
     5401 ctgatggata atcacatccg atcccggaaa acgccgtaaa atagcctgct ctacctgatc
     5461 cgccaccata tgtgcctgaa ccaaaggcag agagtcttcc atttccaaat gaatctgaat
     5521 aaagcgggtc ggccctgact gccgcgtgcg aagatcgtga gcgccgctaa cacccggcca
     5581 ggaagtcacg atatcaataa tttcttgccg ttcttcatca ggcaatgcgc gatccagtaa
     5641 tgactgtacc gcctcatatc ccatgcgtaa cgcgctatat aaaatataga tgccgattcc
     5701 caatgcaaac agagcatcgg cgcgatgcca gccgtaccaa gacaacccca gcgccagcag
     5761 aattgcgccg ttcatcataa catcagactg gtaatgtagc atatcagccc gcaccgcctg
     5821 gctttgcgtc cggcgcacca cccaccgctg aaacgagaca aggataatcg tacaaattag
     5881 cgccacaatt gtcacgataa ccccgacgcc tggatctgtc atcggtgttg gagataccag
     5941 atgttgaata cccgtcaaaa acaggaatag tgccgaaccg gagataaaca tactttgcgc
     6001 cagcgccgcg agggactcag ctttaccgtg accaaacgag tgattatcgt cggcaggttg
     6061 cagggaatat cgcaccacca gtaaattcgt caacgacgcg ccgatatcca ccagcgaatc
     6121 caccagcgcg gcgagaatac tcaccgaccc ggtataccac catgcaaaaa ttttaatcag
     6181 caatagcaac gaagccatcg ccgtcgcagc aatcgccgcc cgactgacca gccgtccata
     6241 agattgattc ataaatactc ccgctatcaa ctgacgctag tataacggaa gcaaatcatc
     6301 tgcaatgcat taagcagcag gcaaattgag gataaaaaaa accccacatc atgtggggga
     6361 agacagggat ggtgtctatg gcaaggaaaa cagggtttac tactgggaac gtgagttgct
     6421 actactcaat agcttcaacg atgaactttt ttgccattgc gtcacgtcac gcaactgctc
     6481 cattcgttgt tgatgtttct cgtttaaaac cgcttgctgc tccggcgtta acaggcgata
     6541 catttggttg cggacttttg ccatctcaac ctgacgagca atttgctcat tcgccatttt
     6601 ttctgcctgt gcgcgcacag cgttttcatc aaaattttct gcggtgacaa ggcgatgcat
     6661 tgtctccagt tcgctaacat taacaggagg ctgttcgtgc cgggcctgtt gcataagatc
     6721 tcgcatctgc tgacgctgat gttcggttaa acttatgccg tcgaacatat ggctctgcgt
     6781 actgcgctgc gtaagttctt cacccggatg ccagttatcg cctgaaccga cttcagcagc
     6841 gtggcttaat gaactgactg ccagcgttga ggccatgacg gcagcggtaa ctatgcgcat
     6901 catttgctcc caaaatcttt ctgtcgcgat tcaacgatag agagtttacg attcaggctg
     6961 caaacatgcg tcagggggtg taaaacaacg taaagtcatg gattagcgac gtctgatgac
     7021 gtaatttctg cctcggaggt atttaaacaa tgaataaaat cctgttagtt gatgatgacc
     7081 gagagctgac ttccctatta aaggagctgc tcgagatgga aggcttcaac gtgattgttg
     7141 cccacgatgg ggaacaggcg cttgatcttc tggacgacag cattgattta cttttgcttg
     7201 acgtaatgat gccgaagaaa aatggtatcg acacattaaa agcacttcgc cagacacacc
     7261 agacgcctgt cattatgttg acggcgcgcg gcagtgaact tgatcgcgtt ctcggccttg
     7321 agctgggcgc agatgactat ctcccgaaac cgtttaatga tcgtgagctg gtggcacgta
     7381 ttcgcgcaat cctgcgccgt tcgcactgga gcgagcaaca gcaaaacaac gacaacggtt
     7441 caccgacact ggaagttgat gccttagtgc tgaatccagg ccgtcaggaa gccagcttcg
     7501 acgggcaaac gctggagtta accggtactg agtttaccct gctctatttg ctggcacagc
     7561 atctgggtca ggtggtttcc cgtgaacatt taagccagga agtgttgggc aaacgcctga
     7621 cgcctttcga ccgcgctatt gatatgcaca tttccaacct gcgtcgtaaa ctgccggatc
     7681 gtaaagatgg tcacccgtgg tttaaaacct tgcgtggtcg cggctatctg atggtttctg
     7741 cttcatgata ggcagcttaa ccgcgcgcat cttcgccatc ttctggctga cgctggtgct
     7801 ggtgttgatg ttggttttga tgttacccaa gctcgattca cgccagatga ccgagcttct
     7861 ggatagcgaa cagcgtcagg ggctgatgat tgagcagcat gtcgaagcgg agctggcgaa
     7921 cgatccgccc aacgatttaa tgtggtggcg gcgtctgttc cgggcgattg ataagtgggc
     7981 accgccagga cagcgtttgt tattggtgac caccgaaggc cgcgtgatcg gcgctgaacg
     8041 cagcgaaatg cagatcattc gtaactttat tggtcaggcc gataacgccg atcatccgca
     8101 gaagaaaaag tatggccgcg tggaactggt cggtccgttc tccgtgcgtg atggcgaaga
     8161 taattaccaa ctttatctga ttcgtccggc cagtagttct caatccgatt tcattaactt
     8221 attgtttgac cgcccgctat tactgctgat tgtcaccatg ttggtcagta cgccgctgct
     8281 gttgtggttg gcctggagtc tggcaaaacc ggcgcgtaag ctgaaaaacg ctgccgatga
     8341 agttgcccag ggaaacttac gccagcaccc ggaactggaa gcggggccac aggaattcct
     8401 tgccgcaggt gccagtttta accagatggt caccgcgctg gagcgcatga tgacctctca
     8461 gcagcgtctg ctttctgata tctctcacga gctgcgcacc ccgctgacgc gtctgcaact
     8521 gggtacggcg ttactgcgcc gtcgtagcgg tgaaagcaag gaactcgagc gtattgaaac
     8581 cgaagcgcaa cgtctggaca gcatgatcaa cgatctgttg gtgatgtcac gtaatcagca
     8641 aaaaaacgcg ctggttagcg aaaccatcaa agccaaccag ttgtggagtg aagtgctgga
     8701 taacgcggcg ttcgaagccg agcaaatggg caagtcgttg acagttaact tcccgcctgg
     8761 gccgtggccg ctgtacggca atccgaacgc cctggaaagt gcgctggaaa acattgttcg
     8821 taatgctctg cgttattccc atacgaagat tgaagtgggc tttgcggtag ataaagacgg
     8881 tatcaccatt acggtggacg acgatggtcc tggcgttagc ccggaagatc gcgaacagat
     8941 tttccgtccg ttctatcgga ccgatgaagc acgcgatcgt gaatctggcg gtacaggatt
     9001 ggggctggcg attgttgaaa ccgccattca gcagcatcgt ggctgggtga aggcagaaga
     9061 cagcccgctg ggcggtttac ggctggtgat ttggttgccg ctgtataagc ggagttaaac
     9121 tccgcatttg taggcaggat aaggcgttta cgccgcatcc ggcatttgag caggatgcct
     9181 gatgcgacgc tgatagcgtc ttatcaggcc tacactccgg gggttatttt ccccacaacc
     9241 ggcgggaaaa atcttcgatc ttgccgctca gtcggcgctt ttgcatcgtc cgcgtccagc
     9301 ttttcgataa cccggcggcg gagagtaaac ggtgatactg atcgtcatca aacggcatat
     9361 gccatgcgat ggcggcagcc tcctgcacgg tcacatcgct gacacgggaa accaactcca
     9421 gcggcgcgtc cgcagacact ttccctggcg ctatcacgct atacaaccac cccaccttac
     9481 ctgtgttttg catcaactgc gctatatcgc tgatatcaaa atggtaattg agtttatagc
     9541 aaggcgagcg cggctggctg acctgaatta atgcctctcc ccagcggaaa atatcgccca
     9601 tatgaacatt actttccgtg agaccgtcgg ttgagagatt ttcgccaaac gcaggcgcaa
     9661 caaacaactc cgcctgttcc ggaaattccc gcacccagta gagatagtgc tcacgaggat
     9721 aatgacacag cgctctgtct ggcccgccgt gaaccttttt ctccgcctgc tcgtcacctt
     9781 ccagccccag ctccgtcagc atcaactcac catcaacctg gattttggca attgcgctgg
     9841 gtttgccttc gggataagcc tgaatcttgc ctgtgtatac atcaaccgga tatcgcatct
     9901 gttccctctc cacgagaaat ttccccatta gaccatattt attttatgtc agccgtgctg
     9961 ttttcgcggt aagtatccag gcaggaactc aatagcaaac cggacgaacg ttagttcatc
    10021 cggctgtggg ga
//
LOCUS       DAZOYF010000142         9937 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZOYF010000142 DAZOYF010000000
VERSION     DAZOYF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.143"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACHUOF_003273"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACHUOF_003273"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR0612327.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACHUOF_003274"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACHUOF_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR0612328.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACHUOF_003275"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACHUOF_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR0612329.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="ACHUOF_003276"
     CDS             complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="ACHUOF_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR0612330.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="ACHUOF_003277"
     CDS             complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="ACHUOF_003277"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR0612331.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="ACHUOF_003278"
     CDS             complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="ACHUOF_003278"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR0612332.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="ACHUOF_003279"
     CDS             complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="ACHUOF_003279"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR0612333.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="ACHUOF_003280"
     CDS             complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="ACHUOF_003280"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR0612334.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8990..>9937)
                     /gene="lpfD-O113"
                     /locus_tag="ACHUOF_003281"
     CDS             complement(8990..>9937)
                     /gene="lpfD-O113"
                     /locus_tag="ACHUOF_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR0612335.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2563 a         2587 c         2295 g         2492 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttcct
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
     3961 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
     4021 tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt
     4081 aactttgtgt aattttagga atttataaag ttatatataa caaatcccaa taattaagtt
     4141 attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag tttatccagc
     4201 tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat aaactcacaa
     4261 atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag cgcggtaagt
     4321 acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg aacaatacct
     4381 tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc
     4441 atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac
     4501 tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag
     4561 attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata
     4621 accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca
     4681 tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga
     4741 tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg
     4801 cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata
     4861 tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga
     4921 tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact
     4981 caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca gcctgctgca
     5041 tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa
     5101 tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg
     5161 ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt
     5221 aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc
     5281 actgcacgcg ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaagg
     5341 cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc atgcccactt
     5401 tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa
     5461 tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca
     5521 gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt ttggcgatat
     5581 ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct
     5641 gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtacctga
     5701 tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc gcatccggaa
     5761 aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagg
     5821 acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc cgcaaacggg
     5881 ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg
     5941 ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc ggcaatacgg
     6001 gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag cgtaatcgcg
     6061 gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc
     6121 accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa cagcgccagc
     6181 gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat ggtgtagaca
     6241 aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa gcgaatcact
     6301 tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc
     6361 ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag ggcgttcgcc
     6421 agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata
     6481 ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa caggccgaag
     6541 gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc
     6601 attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc
     6661 gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga
     6721 aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt
     6781 ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc atgtacagcg
     6841 aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga
     6901 gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa
     6961 cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca
     7021 tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga
     7081 tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg
     7141 cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga
     7201 acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca
     7261 gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga
     7321 acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca
     7381 acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc cactctttgg
     7441 tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg atcagagaga
     7501 caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga
     7561 aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca gccataagtt
     7621 actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac
     7681 cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt
     7741 cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc
     7801 gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cctctgtgcc
     7861 ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca
     7921 tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc
     7981 ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat
     8041 cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat aaccaattgc
     8101 acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat
     8161 cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc
     8221 caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat
     8281 gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt
     8341 cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga
     8401 cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa
     8461 cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac
     8521 ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt
     8581 attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt
     8641 tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca tcgataaggt
     8701 cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat
     8761 tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata
     8821 tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta ctgattttaa
     8881 atgtgaagat gaaaattcca catgccatct ttttgtcatg atgcatttaa aagtgataaa
     8941 gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat tagcgcacaa
     9001 tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga gctccagtaa
     9061 ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac actgttgatt
     9121 tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta tttttgtcgt
     9181 aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc gtattgggtt
     9241 cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg ttttcacact
     9301 caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca acagatgaaa
     9361 aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta tttatttcgc
     9421 aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc tgtgagtaat
     9481 ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt tcaggaataa
     9541 tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta gccccactgg
     9601 atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac gggttgttag
     9661 catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata gcaatcccaa
     9721 catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca gcataagtta
     9781 atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg ttaccagtat
     9841 cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta aatgtcgttc
     9901 caaccttatt ttcgttcgcg gtaataagat atcccga
//
LOCUS       DAZOYF010000143         9826 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZOYF010000143 DAZOYF010000000
VERSION     DAZOYF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.144"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            383..1501
                     /locus_tag="ACHUOF_003282"
     CDS             383..1501
                     /locus_tag="ACHUOF_003282"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR0612336.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="ACHUOF_003283"
     CDS             1567..1815
                     /locus_tag="ACHUOF_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFR0612337.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="ACHUOF_003284"
     CDS             1880..2248
                     /locus_tag="ACHUOF_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0612338.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFCPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHUOF_003285"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHUOF_003285"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR0612339.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHUOF_003286"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHUOF_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR0612340.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHUOF_003287"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHUOF_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR0612341.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHUOF_003288"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHUOF_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR0612342.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHUOF_003289"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHUOF_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2407 a         2721 c         2378 g         2320 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gcctttttgc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagctg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg caggtagact ggccccctga
     9781 atctccagac aaccagtatc acttatttaa gtgatagtct taatac
//
LOCUS       DAZOYF010000144         9774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZOYF010000144 DAZOYF010000000
VERSION     DAZOYF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.145"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="ACHUOF_003290"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="ACHUOF_003290"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR0612343.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="ACHUOF_003291"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="ACHUOF_003291"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR0612344.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="ACHUOF_003292"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="ACHUOF_003292"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR0612345.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="ACHUOF_003293"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="ACHUOF_003293"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR0612346.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="ACHUOF_003294"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="ACHUOF_003294"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR0612347.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="ACHUOF_003295"
     CDS             complement(6031..6360)
                     /locus_tag="ACHUOF_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR0612348.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHUOF_003296"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHUOF_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR0612349.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="ACHUOF_003297"
     CDS             7998..8171
                     /locus_tag="ACHUOF_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR0612350.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHUOF_003298"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHUOF_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR0612351.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2286 c         2433 g         2492 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atct
//
LOCUS       DAZOYF010000145         9753 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZOYF010000145 DAZOYF010000000
VERSION     DAZOYF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.146"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(116..1456)
                     /gene="gudD"
                     /locus_tag="ACHUOF_003299"
     CDS             complement(116..1456)
                     /gene="gudD"
                     /locus_tag="ACHUOF_003299"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR0612352.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(1477..2817)
                     /locus_tag="ACHUOF_003300"
     CDS             complement(1477..2817)
                     /locus_tag="ACHUOF_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR0612353.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(2819..4171)
                     /gene="gudP"
                     /locus_tag="ACHUOF_003301"
     CDS             complement(2819..4171)
                     /gene="gudP"
                     /locus_tag="ACHUOF_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR0612354.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(4605..5054)
                     /locus_tag="ACHUOF_003302"
     CDS             complement(4605..5054)
                     /locus_tag="ACHUOF_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR0612355.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(5072..5854)
                     /gene="truC"
                     /locus_tag="ACHUOF_003303"
     CDS             complement(5072..5854)
                     /gene="truC"
                     /locus_tag="ACHUOF_003303"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR0612356.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(5854..6183)
                     /locus_tag="ACHUOF_003304"
     CDS             complement(5854..6183)
                     /locus_tag="ACHUOF_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR0612357.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(6805..7350)
                     /gene="syd"
                     /locus_tag="ACHUOF_003305"
     CDS             complement(6805..7350)
                     /gene="syd"
                     /locus_tag="ACHUOF_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR0612358.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            7418..8266
                     /gene="queF"
                     /locus_tag="ACHUOF_003306"
     CDS             7418..8266
                     /gene="queF"
                     /locus_tag="ACHUOF_003306"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR0612359.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            8378..9742
                     /gene="ppnN"
                     /locus_tag="ACHUOF_003307"
     CDS             8378..9742
                     /gene="ppnN"
                     /locus_tag="ACHUOF_003307"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR0612360.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         2432 a         2661 c         2300 g         2360 t
ORIGIN      
        1 ttaaattcaa tgaattaaaa aatgatgtag acctgataca tagcacaaca ggctattttg
       61 cgtttagcat cagtctcaaa ccggctccag atagagccgg ttttggtttt ctgtcttaac
      121 gcaccatgca cgggcgcttg ttatcgaacg tccagccagg aatcagatac tgcattccca
      181 tcgcatcgtc acgcgcgcca agcccgtgtt tctgatacag ctcatgggct ttcatcactt
      241 gatccatatc gatttctaca cccagccccg gtttttctgg cacctgtacc agcccgcctt
      301 tgatctcaaa cggttctttg gtcaggcgct gattaccttc ctgccagatc cagtgcgtat
      361 caatagcagt aattttaccc ggtgcagcgg cggcaacatg ggtaaacatc gccagggaaa
      421 tatcgaagtg gttgtttgag tgtgaacccc aggtcaggcc aaattcatgg cacatttgcg
      481 ccacacgtac cgaaccttgc atagtccaga aatgcggatc cgccagcggg atatcaacgg
      541 attgtaacga gagcgtatgg cccatctgcc gccagtcggt ggcgatcata ttggttgcag
      601 tcggcaggcc agtcgcgcga cggaactctg ccatcacttc acgcccggag aaaccttgct
      661 ccgcaccaca cggatcttct gcataagcca gcgaaccttt caggtattta ccaattttaa
      721 ttgcttcgtt cagcgaccag gcaccgttag gatcgagcgt aatacgcgcc tgcgggaagc
      781 gttgcgccag tgccacaata gactcggctt cttcttcccc ggccagtaca ccgcctttca
      841 gtttgaaatc gttgaagcca tatttttcat acgccgcttc cgccaggcgc accaccgcat
      901 ccggcgtcat cgcttcttca tgacgcaggc gataccagtc gcatgagtca tccggctggc
      961 tttgatacgg cagaggtgtg gctttgcgat taccgacgaa gaacagataa ccgagcattt
     1021 cgacttcgct acgctgttga ccatcgccca gcagcgatgc cacgtttacc cccagatgct
     1081 gccccagcag atccagcatt gccgcttcta tcccggtaac tacatgaata gtggtacgca
     1141 aatcaaatgt ctgcaaaccg cgcccactag catcacgatc ggcaaaagta ttacgcacca
     1201 gcgtcagaac gtttttgtat tcacccagcg ttttacctac caccagcgga atcgcatctt
     1261 ccagcgtttt acggattttc tcgccgccgg gaatttcccc tacgccagtg tgaccggaat
     1321 tatctttgat aatcacaata ttacgcgtga agaacggtgc gtgtgcacca ctcagattca
     1381 tcagcatact gtcatgaccc gccaccggga tgacctgcat ttcagtaaca acaggcgtcg
     1441 taaattgaga actcatattt atgtcctttt tcagaatcaa tgacggccga aaacgggacg
     1501 tttacggtca aaggtccagc cggggatcag gtactgcatc ggacctgcgt cgttacgcgc
     1561 accgcaaggc agacgtttat aggcctcatg tgccttttgt acctgttccc agtccagttc
     1621 cacgcccaga ccgggcgcat caggaacggc aatttttccg tttttaatct ccagcggatt
     1681 tttggtcagg cgacaatcgc cctcctgcca aatccagtgg gtatcgatag cggtaggatt
     1741 acccggtgcc gccgcgccca catgggtaaa tatcgccaga gagatatcga aatggttgtt
     1801 ggaatggcag ccccaggtca gcccccagtc gtcgcaaagc tgcgccacgc ggactgcacc
     1861 ggaaagcgtc cagaagtgcg gatcggcaag tggaatatct accgcattga gcatcaccgc
     1921 atgacccatt tcgcgccagt tggtggcgat catgttagtc gcgacgggca agccggtcgc
     1981 ccgtcgaaac tccgccatca cttcacgtcc ggagaagccc tgttctgcgc cgcatggatc
     2041 ttcggcatag gtaagaacat cattcagccc tttgcataaa gaaatggctt catcaagcag
     2101 ccatgcaccg ttgggatcaa cggtaatccg cgcatccggg aagcttttct tcaatgcacg
     2161 aacagtgtcg atttcttgct cgccaggtaa caccccgccc ttaagtttga aatctttaaa
     2221 gccatagcga tcctgtgagg cttccgccag acgcacaacg gcttcgctgt tcatcgcttt
     2281 ctgatggcgc aactgatacc actcatggtt gcccggcgta ttttccagat aaggaagatc
     2341 ggttttggtc cgatcaccta tataaaacag ataaccgagg acggtaatag tctcgcgttg
     2401 cttgcctggc cccaacagtt cgcagaccgg aacattcagc gccttaccta gcaggtcaag
     2461 caaggcggct tccagcgccg ccacggcatt aacgcgcaat tcaaaagtcc aggcaccttt
     2521 gccaaaggta tcaaaatcgg ctgcctgatt acctttatgt acctgctgaa ccactttatt
     2581 caggcgcgca acttcctggc ccagaaccat cggaatagca tcgaccagtg tttgataaat
     2641 cacctctccg cccggcgctt caccaatgcc ggtatgcccg gcgttatcgg tgagtaccac
     2701 gatattgcgg gtgaagtatg cgttatgcgc accaccaata ttgagcaaca tgctgtcatg
     2761 tccggcgacc ggaatgactt tcatatcagt aataacagga ctggattgtg tcgccatctt
     2821 attgccccgc gacaggtttc agctcgatac gcttgatatc gcccaccagc accaggtagc
     2881 tcagtaccgc gattagggca tgaacaccaa cataaatcag cgccccatta aacgagccag
     2941 tcgtgccaac gatataacca attgcgattg gcgtgacgat gccagaaatg ttaccgaaca
     3001 tgttgaacag gccaccggaa agaccgctga tctcttttgg cgcggtatct gccattactg
     3061 cccaacccag cgccccgatg cccttaccga agaaggccag cgccataaag ccgatgatca
     3121 tccactcaac gttgacgtag ttgcagaaca ccatcaccat cgacagcaac atgcccatta
     3181 cgatcggtgt tttacgcgca atgttcagcg atcccgtgcg gcgcatcagc cagtcggaaa
     3241 taattccacc cagcacaccg ccgataaaac cgcaaaccgc cggaacggaa gccacaaagc
     3301 ccgctttcag aatcgacatc ccacgcgcct gcaccagata aaccggaaac caggtaatga
     3361 aaaagtaagt cagggcgttg atacagtact gaccgatata aacgccgatc atcatccgtg
     3421 acccgagcag ctgtttgatc tgcccccact tcacgctgaa cggcacttta actttggtgc
     3481 tttgctgatc catattgatc agcgcgccac ccgcggcgat atactccagc tctttctgat
     3541 ttacccccgg atgttgattt ggctcgtgga tgactttcaa ccagataaag ctgatgacaa
     3601 tgcccagacc gcccataaag aagaagacgt gtgaccagcc cacttcatgc gtcagccagc
     3661 ccataatcgg cgcgaagatc accgttgcga agtattgagc ggagttaaaa atcgacaccg
     3721 ccgttcccct ttcctgcacc ggaaaccagg ccgcaacaat gcgactgttg ccggggaaag
     3781 atggcgcttc agcaagcccg accaggaagc gcagagtaaa cagggcaacg ataatgccga
     3841 atccactaaa gatatcgacg aagccttgca gcaaggtaaa catcgaccag ataaagatcg
     3901 accagaagta gacgcgtttt gaaccaaaac gatccagcaa ccaaccacca gggatctgcc
     3961 cgataacata agcccatgag aaggcagaga acacatagcc cattcccacg ggatcaaggc
     4021 cgatatcttt ggccatttcc gaaccggcaa tagaaagcgt agcgcggtcg ccgtagttga
     4081 aggatgtgac gataaacaac atcaccacta tccagtaacg agcattcgtg cgtttttcca
     4141 cactgctcgc agcctgactt aaagaactca ttgttgcact cctgaaaatt cgcgttagcc
     4201 acgctcactc tggactgcga catcgccagg aaatcagagg tgacgtaggg tgttttttgc
     4261 cgtttttata ggtcgttcgc cgaatacggc gcgtgtttat atctggcaat agcagtataa
     4321 aaagcgcgcc atagcggctc accgtgcaac aacacaacat taatgcgttc aatgaggccc
     4381 gattttggca ttagccctgg acggtggaat ccacttcacg gaaatgaaaa caagaacaag
     4441 aaaggaaggg taaaaacgaa gaaataaaga agagtatgaa atggatcgct tgactctagg
     4501 caaacgccag taaaaatccg cgctatgaag cagtttttac tggcatttgc ctgaaaagat
     4561 tcgattcagc accgctaaaa cgacatttac cgctcgctga catatcagga caacagcgtg
     4621 ccccactgtt cgacccacgg atttgattcc gtttccggtt ccgggttctc actggcgtca
     4681 atcaacagca tttcgccaac ccgctgagcg ctctgttcct gcaacaaggc atcgaactgt
     4741 ttgccgccat tgcaaaaatt tacataactg ctgtcgccga gcgcaatcac gccataacgc
     4801 agattcggct ggaagcccag actatctttg attccctgaa agagcggcac aatgctatca
     4861 ggaaggtcgc cctgcccggt cgtggacgta accaccagaa catacttatc ctgatagggc
     4921 agccagtcgc ttaattcagg atcttcaaat accgttgctt tgtggccctg tgcggtcaga
     4981 attgcttctg cttcttccgc cactaacagc gaatttccgt acatggtgcc gacaaaaata
     5041 ccaatttccg ccataccgtt ttctccctgg attaggaact tatctctcca tcctgacccg
     5101 atggcgcact gaactcaacc ctttcatttt caggaagcag accgcgccag ccaaattgtg
     5161 ataacgcctg catccaggtg tcgtccaggc ccgcgtgaat agtcagcggc tcgccagtaa
     5221 aaggatgcgt caatgacaac tgactggcat gtagcattaa ccgctggagg ccaaaatgct
     5281 cagcaccgct gcgattctgg cgtaaatcgc catgtttgct atcgccaata atcggatgac
     5341 gcaaatgggc aagatgtcgt cgaagctgat gtttgcgtcc ggttttcggc tccagttcca
     5401 ccaggccgta gcgcgtggtc gggtaacgtc cggtcgctac cggcatttct acggtcgcca
     5461 gaccgcgata atgcgtcact gctggctgcg ggcctttatc ttcgcgggca aatttatcag
     5521 cgattttgtc cagttcttcc accagtggat aatccaacac cgcttcttcc atcaaccagc
     5581 cgcgcacaat cgcatggtaa cgtttctgga tttggtgctg ttcaaactgt tgtgccagca
     5641 gccgtccggc ctcgctggat aatcccatca acaacacacc agaagtgggt cggtccagac
     5701 gatgagcagt aaaaacatgc tgtcctatct ggtcacgcac ggtttgcatg accactactt
     5761 tctcgtcgcg atccagccag ctacggtgaa ccagccagcc ggagggttta tttaccgcaa
     5821 ccagccattc atcctgatag agtatttcca gcattagctc gcatcatccg caaaaagagc
     5881 atccagtttt tccaactcag ccagaataag cgcgcgttgc ggatggtccg tcgccagcgc
     5941 catttcataa tagggtgcaa cagcaaaagc gcccggtaac ggctggttgt tatttaacaa
     6001 atcgtgcatt cgcgggatca gcccccactg caaccactcc agtggttcca tggtgtccat
     6061 aaagaacggt tgggtactat taaattgatg cggctggggt tcatcgtttc gccagtgctg
     6121 atgttcacgc agtaacgctt caagcgcctg caactggaga cgaacgcggt catgagtggt
     6181 cataaagcaa cctcaataag aaaaactgcc gcgaaggata gcaggaataa aaaaagggag
     6241 cactgtattc acagtgctcc cggttcgttt cgcagcattc cagctacttt tgttgctccc
     6301 tgctcatcct tgacaacttt tcctctggcc ttgcggccaa tcgttcatcc tgaactattg
     6361 cttcctgctc acaccacccc gatgtgatac ttcatcctga agtgtccctg gccttcctga
     6421 cccaccgaat catcctgacc ggttctcatt ctccatcctg gaggtgtcct ttaacgcgtc
     6481 ctgcgtcatc ctcttcgctt catccagaag cctttccctg aaacaccatc ctggtgtgtc
     6541 ctgcagaagt gtcatcatcc tgatgttcac ttcgttgtct gactccctgt cgacgaagat
     6601 agaatcgtct ttttcgcgaa gtcttacaag gcgcttacag acaatgcctt aaggaaaatt
     6661 tcatatgaaa gttaagaatt aacaatcaac attttgattt taaatgaaaa taacgattct
     6721 tatcttttta agtattcgct tatttcctgg cgatctccta cagagtctgt aagagatctc
     6781 tcacaaccag atggggtatt tggattacag aagaggctta agttgattaa ggaattccgc
     6841 aagatttgag gctaaattcg tccgtttacg tgttcccagc gtctctttgc acacttcacc
     6901 ggagagatta cacaccgaga tgacctctaa ctcttcttcc agcgtcgcga taaatagcgt
     6961 gggcggcagt tttagccttt tctgagtcac cagatgacca atcagatttt cctgcacccg
     7021 acggaagtcg tcttcactcc aggtttgcag caatgtcagt ttgatatcgc caaactgcgc
     7081 gtgcatatcc ccggcaaact gagtcgtata aaaggtatga attgtgggtt gtatcacaat
     7141 atcaaaagcg cgttcaaccg cgtttacatt ttgttccccg gtaaacggct ggggttgcca
     7201 gtatacggca tcttcggtag ttgaaataat gcacggtgaa ggaacaccgt acaattcctc
     7261 acttaacggc cagcttttat gctcttcatg ccacgcatcg cagtagcgtg cagtaaaatc
     7321 tttcagggct tgtgcggtca aatcgtccac taatttctct cttcacttaa accagataca
     7381 cttgtcgttt agtttatctg gtttatgacg gtgaaacatg tcttcttatg caaaccatca
     7441 ggcacttgcg ggcctgactc ttggaaaatc aaccgattac cgggatacct atgacgccag
     7501 cctactgcaa ggcgttccac gcagcctgaa tcgcgacccg ctgggtctga aagcggataa
     7561 cctgcctttt cacggtacgg atatctggac gctgtatgaa ctttcctggc tgaatgcgaa
     7621 aggtttgccg caggtcgctg tcggtcatgt tgaacttgat tacaccagcg taaatctgat
     7681 tgagtcgaag agttttaagc tctatctcaa cagttttaac cagacgcgtt ttaataactg
     7741 ggatgaggtg cgccagacgc tggagcgcga cttaagcact tgcgctcagg gtaagattag
     7801 cgtggcgtta tatcgtcttg atgaactgga aggccagccg ataggtcatt tcaatggcac
     7861 ttgcattgat gaccaggata tcactatcga taactatgaa ttcactactg actatctgga
     7921 gaatgccacc agcggtgaaa aagtagtgga agagacgctc gtcagccacc tgctgaaatc
     7981 aaactgcctg atcacccatc aaccagattg gggttcgatc caaattcagt atcgtggacg
     8041 ccaaattgac agagaaaaac tgctgcgtta cctggtctca ttccgtcatc acaacgagtt
     8101 ccacgaacag tgcgtggaac gcatctttaa tgacctgtta cgcttctgcc agccagagaa
     8161 attgagcgtt tacgcacgtt atacccgtcg tggcggtctg gacattaacc cgtggcgcag
     8221 taatagcgat tttgtcccat cgaccacaag actggttcgg caataaattt tttctcaatt
     8281 ttgcgtgctg gattcacgca gaaggttgtg aaaggtcatc aggcagggct attgtaatca
     8341 aagggaatga cgatattcgt cccataagga gtttttcttg attacacata ttagcccgct
     8401 tggctccatg gatatgttgt cgcagctgga agtggatatg cttaaacgca ccgccagcag
     8461 cgacctctat caactgtttc gcaactgttc acttgccgta ctgaactccg gtagtttgac
     8521 cgataacagc aaagaattgc tgtctcgttt tgaaaatttc gatattaacg tcctgcgccg
     8581 tgaacgcggc gtaaagctgg aactgattaa tcccccggaa gaggcttttg tcgatgggcg
     8641 aattattcgc gctttgcagg ccaacttgtt cgcggttctg cgagacattc tcttcgttta
     8701 cgggcaaatc catcataccg ttcgttttcc caacctgaat ctcgacaact ccgtccacat
     8761 cactaacctg gtcttttcca tcttgcgtaa cgctcgcgcg ctgcatgtgg gtgaagcgcc
     8821 aaatatggtg gtctgctggg gcggtcactc aattaacgaa aacgagtatt tgtatgcccg
     8881 tcgcgtcggg aaccagctgg gcctgcgtga gctgaatatc tgcaccggct gtggtccagg
     8941 agcgatggaa gcgccgatga aaggtgctgc ggtcggacac gcgcagcagc gttacaaaga
     9001 cagtcgtttt attggtatga cagagccgtc gattatcgcc gctgaaccgc ctaacccact
     9061 ggtcaacgaa ttgatcatca tgccggatat cgaaaaacgt ctggaagcgt ttgtccgtat
     9121 cgctcacggc atcattatct tccctggcgg tgtgggtacg gcagaagagt tgctgtattt
     9181 gctgggaatt ttaatgaacc cggccaacaa agatcaggtt ttaccattga tcctcaccgg
     9241 cccgaaagag agcgccgact acttccgcgt actggacgag tttgtcgtac atacgctggg
     9301 cgaaaacgcg cgccgccatt accgcatcat cattgatgac gccgctgaag tcgctcgtca
     9361 gatgaaaaaa tcgatgccgc tggtgaaaga aaatcgccgt gatacaggcg atgcctacag
     9421 ctttaactgg tcaatgcgca ttgcgccaga tttgcaaatg ccgtttgagc cgtctcacga
     9481 gaatatggct aatctgaagc tttacccgga tcaacctgtt gaagtgctgg ctgccgacct
     9541 gcgccgtgcg ttctccggta ttgtggcggg taacgtaaaa gaagtcggta ttcgcgccat
     9601 tgaagagttt ggtccttaca aaatcaacgg cgataaagag attatgcgtc gtatggacga
     9661 cctgctacag ggttttgttg cccagcatcg tatgaagttg ccaggctcag cctacatccc
     9721 ttgctacgaa atctgcacgt aatctcccct ccc
//
LOCUS       DAZOYF010000146         9704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZOYF010000146 DAZOYF010000000
VERSION     DAZOYF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.147"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHUOF_003308"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHUOF_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR0612361.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="ACHUOF_003309"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="ACHUOF_003309"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFR0612362.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="ACHUOF_003310"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="ACHUOF_003310"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR0612363.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="ACHUOF_003311"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="ACHUOF_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR0612364.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="ACHUOF_003312"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="ACHUOF_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR0612365.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="ACHUOF_003313"
     CDS             4025..4615
                     /locus_tag="ACHUOF_003313"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR0612366.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="ACHUOF_003314"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="ACHUOF_003314"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR0612367.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="ACHUOF_003315"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="ACHUOF_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR0612368.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="ACHUOF_003316"
     CDS             complement(6551..6658)
                     /locus_tag="ACHUOF_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFR0612369.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHUOF_003317"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHUOF_003317"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0612370.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="ACHUOF_003318"
     CDS             7648..7875
                     /locus_tag="ACHUOF_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR0612371.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHUOF_003319"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHUOF_003319"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR0612372.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..>9704)
                     /locus_tag="ACHUOF_003320"
     CDS             complement(9447..>9704)
                     /locus_tag="ACHUOF_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612373.1"
                     /translation="IKAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYG
                     YLTNTGNHYDVYLPPELFSQAYKLKNKEMNAQLDYLNRYAT"
BASE COUNT         2210 a         2527 c         2561 g         2406 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taat
//
LOCUS       DAZOYF010000147         9537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZOYF010000147 DAZOYF010000000
VERSION     DAZOYF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.148"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACHUOF_003321"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACHUOF_003321"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR0612374.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACHUOF_003322"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACHUOF_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR0612375.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACHUOF_003323"
                     /pseudo
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACHUOF_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4131..4886
                     /gene="actS"
                     /locus_tag="ACHUOF_003324"
     CDS             4131..4886
                     /gene="actS"
                     /locus_tag="ACHUOF_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR0612376.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="ACHUOF_003325"
     tRNA            4965..5038
                     /locus_tag="ACHUOF_003325"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5173..6783
                     /locus_tag="ACHUOF_003326"
     CDS             5173..6783
                     /locus_tag="ACHUOF_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0612377.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6815..7234
                     /locus_tag="ACHUOF_003327"
     CDS             6815..7234
                     /locus_tag="ACHUOF_003327"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612378.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKVSWR"
     gene            8353..9099
                     /locus_tag="ACHUOF_003328"
     CDS             8353..9099
                     /locus_tag="ACHUOF_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0612379.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9397..>9537
                     /locus_tag="ACHUOF_003329"
     CDS             9397..>9537
                     /locus_tag="ACHUOF_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612380.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLP"
BASE COUNT         2432 a         2362 c         2422 g         2321 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtctagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcttc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccatg
     5041 acacttctta aagtctacta acttactgaa tcctaaggga tacggcttaa cgccttccga
     5101 tagttttact cactgtaacc aacactgtga ctaactttgt tgaagtagtg agcagaccta
     5161 aggtctcagt acatgctcaa gtcaagaacc tacctttatc agcgtaatgg tgtcttttac
     5221 atccgtctta ggatgaagac cactagtcgc ctgaccgctt cgcttccttc tcacaaccgt
     5281 tataaactag cgtcagtatc tttacggacc aaggatagac gtaccgctat ggctcactca
     5341 cggcacatca agtcagcact taaagcaatc catgcagaca accctaacgc ctcttatgag
     5401 gagctacggg agcacctgaa gaccattgtc gagtgggaac ttagtgtcag ccgtgatgac
     5461 ctgaacgacc cggagtccta ccagctttac gttgaccagt acgatgacat taagtccaac
     5521 cttcgggaag ctgtagcgac cgagcgtctg actgtagacc agcatcgcta tattaatgac
     5581 gttatcggtg tgcttaaggc ctgtcaggat agactcaatg gcgatagctc tggcctattg
     5641 tcttaccttg agcctgagac tggcagtcta agaccgtccg tctccctatc tgtattggcg
     5701 gaacctgaag tccctgaacc taaagcactg accctcgcgt ctctcattga gcagtacgag
     5761 caggagaacg cccagaactg gaagccagcg accctaagcg aaaaccgagc gtcacactct
     5821 acgctaatcg agatattcga ctatctggat attcaggatg tcggcaaggc gacccgtgca
     5881 gatatgctca gagttcgtga agtcctccag cagctaccga agaaccgcaa gcaacgcttt
     5941 aagtctatgc cgctgtctga cttgctgaac cgggagtcta agactgactg tctggacgtc
     6001 gttaccatta acaataaata tcttatcaag atggctgctg tgtttaagtg ggcggtacgc
     6061 aacgacttga tagctaagaa cctgaccgaa ggtcttgagc tgaaagtccc tcagcgtaaa
     6121 gcctccgacg ctcgtgatgc gttcagtcct gagcaggtag ggcaactact ggtcgcagct
     6181 aaagcgtact cgcagaagac ctcaggtaag ccataccatt attacgtcac cgctctggct
     6241 gcgattacag gtgcgagact taacgaggtg gcccaacttc aggttaaaga tgtcaggacc
     6301 actgaggctg ggaccgtttt tatccatatc aacgaagacg atagcagcct gccgggtaag
     6361 agtatcaaga acgcccacag tgaccgctgt gtcccgttgg tagatggtgc ttatggtttt
     6421 gtattggctg actttatgtc actcgtggaa gaccgtagaa agactgaggg tgataatgct
     6481 atggtgttca atggcctcaa gctaatgaag aacggctacg gtgagcaggt tagcaagtgg
     6541 tttaaccgaa cgttgctgcc caaagtcctc gctgaccgta gcggcttagc ttttcactcg
     6601 tttagacata cagttgcgac ccagttaaaa caacatgggg tcgagttagc gtatgctcag
     6661 gcgattatgg gtcatagctc agggtctata acttacgacc gttacgcgaa agaggtcgag
     6721 gtggaaacgc tgaaagaaaa attagcggag tcattgtcag taaaaaaaat agacggaaag
     6781 taatcgcata gggatagcgg aatgtgagga cgtaatggtc aaagaatttt tagttagcaa
     6841 agaaaataag ccttcatatg aatgtatgaa tactgctgaa acttgtttgg ggctggctgg
     6901 aatggcttcg gaagattggc aacgtgagca tcattgccgc acaggtataa tatttactgc
     6961 ttttgctatt gaggcaatgt tcatcttcta tagaaaacag gttgaccccg gctacgataa
     7021 gacgcagaaa gagtgcagaa agacaatgca caagaatacg ttaaaattgt gcggtataaa
     7081 caattatatg gggacaaaac cttaccaaat tatcaaagag tgtcttgagg taagagacgc
     7141 tattgctcac ggcgattcgt atacttcgag ctttaatttc tcggcggatc atttggataa
     7201 ccaagacgat attgcaaaag tgtcctggcg gtaaactctg aacaatttcg gggattaact
     7261 gttgagcgcc ttgcaaaata cattgaaatg gcaaagcata ttgaccatga gatttctgat
     7321 aatggttaca ggccttctga aggttacctt ccagcagccg agcgcagaag tctgatgtta
     7381 gcttttggcg tcagtggtgt gtcaatgtgg cccagtgctt gagcgataat acacctcact
     7441 atagaggaat ggacccttaa ctatcactat aggggagaga ccctaagtcc ccaccttaat
     7501 agcagtcacg acccatacac acattcatta agtctcaggc ttccggtagc ggaccgattg
     7561 gaaaccatcc gcagacgcct agtgttaaat caccttggcc tgagaccatt ccttttaacg
     7621 taagctatct tcccttttga caggcgggga gcagtagacc acatccgact ttaagttggc
     7681 tctgtgataa tcgctgcgcg gataactgaa gaaccaagac accattactg aggtctgcca
     7741 tccatttgtg gacaggcgga aactcaaaga ggttagatca gtggtgtctg aggttggagc
     7801 tttgagtctt gaccagcagg ctggaggaga ccatgagttt atcttgttat ggattgtgtt
     7861 atcacaattc caataagaag aactttaagt ccagcctcca ctcccgccaa cccgaagtaa
     7921 aattatggtc gctatgatgt ggtctccatg cttcgggtct tactggaagt actgaacact
     7981 ttcgcgtcaa gttctccgaa gttcattcct gtttgactaa actatcacta tagggaccat
     8041 atctcgcctc tgacctcctg agagacggca agcgagaaac cgtggtccac gctagcaagc
     8101 tagccaccag cctaaggcaa gacagcctct ggtcagcctg tgtgttcttt ggtctcgtga
     8161 cttaagtcta tcaccacaca cgctcggaca tgctgtcgaa ccttcatctc tatggtcatc
     8221 gcagaccact aaccccaatt acctcaagtc attctgtttg cgtcctgttg cctgctggtc
     8281 tccctttacg tgggggcttg agtaggtcag tggggtgcga cttcagggtg caaacaaaag
     8341 gagtacacgt aaatgtccca acaagaaatc agcatcatta acctcgacca gcttgtgtcc
     8401 atgacctcag ttgaaattgc tgagctgaca ggaaaagaac ataaacacgt tttacgagat
     8461 attcgtaata tggtcgaaga gcttaacggg gccaaaactg agcactgctc aactttgagc
     8521 agcgagctta acgggtccaa atttgggctc gttggcgagg aagtatacaa ggacgcgaaa
     8581 ggtgagagcc gaacaatgta tcgtctcgac cgtaagcata ctttcattct ggtcgctggc
     8641 tattccgttc acctccgcgc caagtgttac gaccatattc agacgttaga gcgtcgagtc
     8701 cttcaactgg aagaccagaa gaagcgagca gccattcagt ccgctaaccg ccgaggcgtg
     8761 acttggggcg actactgcaa aacctatggt ctgcccgctc agaagctaat gaccgcactt
     8821 ctccagcatc gtggcctctt tcgtaagaac ccaatcagta acgagtggtc tgtaaatccg
     8881 aagtattccg actgctttcg tatcattaag ccgtcagacc agaagttctc agcaggtggg
     8941 tataacttcc ggttcaacgc taaggggctg gaggtcttcg gtaagcctga gatggttgac
     9001 aagatgcgag gcatccttat tgcattcact ggcaccgacc agcagaagca agagcacctc
     9061 ctgaagctgg cacaatccgg taaggtggag ggaatctaag atgatgattg acccgcgcac
     9121 acctgagggg cgcatgactc tccgttaccg tggctaccgg actgaagtcc tcctcagaga
     9181 gcttggtcta gacccggaag atgagacccg ccagcaccaa tcccgagacg agctaatagc
     9241 ccaactggtc gctatgaaac tgccgcttaa ccgttagtag ctcacctact caatacctaa
     9301 tttaaacacc ttttgagatg accgatgcgg ggagacccgt aggccttact gtgcctgcga
     9361 taccgatagt ctcaactctc aagaggagaa ttacctatga tgttcaacaa caacaactgg
     9421 aagctgtcag tcaccgacat caacctttat gaaaacactg tgagtctgga cggtcagtcc
     9481 tatccgttgt cgctcgctat caagaccctc attccgggct acctgtcagg cttaccg
//
LOCUS       DAZOYF010000148         9514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZOYF010000148 DAZOYF010000000
VERSION     DAZOYF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.149"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(33..935)
                     /locus_tag="ACHUOF_003330"
     CDS             complement(33..935)
                     /locus_tag="ACHUOF_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFR0612381.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(1073..2314)
                     /gene="yihS"
                     /locus_tag="ACHUOF_003331"
     CDS             complement(1073..2314)
                     /gene="yihS"
                     /locus_tag="ACHUOF_003331"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFR0612382.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(2331..3209)
                     /gene="yihT"
                     /locus_tag="ACHUOF_003332"
     CDS             complement(2331..3209)
                     /gene="yihT"
                     /locus_tag="ACHUOF_003332"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFR0612383.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(3233..4129)
                     /gene="yihU"
                     /locus_tag="ACHUOF_003333"
     CDS             complement(3233..4129)
                     /gene="yihU"
                     /locus_tag="ACHUOF_003333"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR0612384.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4297..5193
                     /gene="yihV"
                     /locus_tag="ACHUOF_003334"
     CDS             4297..5193
                     /gene="yihV"
                     /locus_tag="ACHUOF_003334"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFR0612385.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5227..6012
                     /locus_tag="ACHUOF_003335"
     CDS             5227..6012
                     /locus_tag="ACHUOF_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0612386.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6111..6710
                     /gene="yihX"
                     /locus_tag="ACHUOF_003336"
     CDS             6111..6710
                     /gene="yihX"
                     /locus_tag="ACHUOF_003336"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR0612387.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            6704..7576
                     /locus_tag="ACHUOF_003337"
     CDS             6704..7576
                     /locus_tag="ACHUOF_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR0612388.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            7573..8010
                     /gene="dtd"
                     /locus_tag="ACHUOF_003338"
     CDS             7573..8010
                     /gene="dtd"
                     /locus_tag="ACHUOF_003338"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR0612389.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            8055..8996
                     /gene="fabY"
                     /locus_tag="ACHUOF_003339"
                     /gene_synonym="yiiD"
     CDS             8055..8996
                     /gene="fabY"
                     /locus_tag="ACHUOF_003339"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR0612390.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            9412..>9514
                     /locus_tag="ACHUOF_003340"
     CDS             9412..>9514
                     /locus_tag="ACHUOF_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612391.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLN"
BASE COUNT         2248 a         2375 c         2517 g         2374 t
ORIGIN      
        1 cttcaatgat tttgactgct aatcaataca agttattttc tctgtccgta aatattaaaa
       61 aacaggcggt gcggttttcc tggttccagt aataataagt cgatacctga attaaaggcg
      121 ttaggcgggc aactcatagg ttcaactgcc agtccctgac gctgtaattt ttcaccgcta
      181 taaacctgca cccagggttg atcggaacaa aggcttaccg aaagcgcctg ctgtggatgg
      241 gtaatggtca tttcccacaa atcattagcg gttttaaagg tgtggtcgat ttttgtcgcg
      301 gctatttttt tcgcctgcgt gaaatttaaa tccagctcat caacgtggtg aagcgttgtc
      361 ggatttacgt gttcatcgac ggcataaacc tggtttgccg gaagctggaa cagatattca
      421 tcaacagatg ttaagttgca ggttaaataa ggatgaatac ccacgccata aggcgctgcg
      481 acagtgccga tattctggct tgcgatctca acggaaagac ccgtgtgtgc gttgagcgaa
      541 taaacaactt gtgaagccag cataaacgga tagccgtaac tgggcggcag aaaagctgtc
      601 agggtaacgc tggtggtggt taattcacta atttgccagt ctcgccaggc aagcagcccg
      661 tgaatagcgg cttttgaact atgttcatta atgggtaact gatattcctg cccctgataa
      721 cggtaacagc cgttggcgat ccggttcggc cacggaataa gcactttacc cagatgcgcc
      781 agcggcatct cttcgggttt gtgcgggatg actaaatgac aaccctgaaa ggttaattca
      841 gccagaccag caccgacggt cacaatggtc gcgtgataat cccccgcggc aagtgaaacg
      901 gtctgtccac tgcaatgcat attggtgatt tgcatacaag gcaccttaat taacgacata
      961 cagcctcccg tgaggctgat gtcaaaccca aaaactgcac cggacggtcc cctcgcccct
     1021 ttggggagag ggttagggtg aggggaaaac ccgcacccta ttttcagatc agttatttcg
     1081 cattaatatc cagcagaccc gctgcaaccg ctggagccat gccgggcgct aacgggatac
     1141 gcgggatcac caggcaatgc agcaggtgat aaatatcctg tttgccgtcc cagactttgg
     1201 tgatgacctt attgtccgca tccagctcct gccaccagga accattttca tagtccatca
     1261 ggtacttaat gcagtactcc caccatgttt gataccaggt ttcatactgg cgatcgccag
     1321 tgacggtgta gagcgcgtag accgtaccca ttgcttcgac gataggccaa cgtacacgtt
     1381 cgcggaccac cggttttcct tcccagtcaa cggtataaac aatcccgtcc gctccatcgg
     1441 gtgcccaggc atcgcgcacg gtggcgttaa acagaccttt ggcatcttct agcagccatg
     1501 ctggtggttg ttcgcaacgg gcttccaggg cagcatggat gtgcagcatt aaacggcccc
     1561 attcgatcca gtggcctggt gtacctccga acgcgcggaa gcgatgcgcc gggttatctt
     1621 tgttgtaatc cggcagcgga ttccactggg tatcgaagtg ttcgttaacg cgataatgat
     1681 tatttctggc gacgtcgtgg ataatcacgg aagccacgcg aatcgcgcga tccagccatt
     1741 ttttgtcgtg agtgacgtca taaacaatca agaaagcttc caccgcgtgc atattggcat
     1801 tgccgccgcg gtactcttcg gttttgctga aggcttcgtc ccaggactcc aggcacatct
     1861 gctcttcttc gctccagaaa tatttctcga taatctcaat ggtgtaatcg agcagcttgc
     1921 gcgcttccgg gtgacccgtt gtgacggcgc tggcggcacc cagcagagca aagaagtgtt
     1981 gatagccctg tttggaggca tccaccacgc cctcgtcatt cacgcaggca taccagccgc
     2041 catatttttt atcacgcagt gcgccgttca tggctttaat accgtgatca accaacgcgt
     2101 aagcgccagg tcgacccatc gccgcagcaa cggaataaac gtgcaacata cgagctgtga
     2161 tccacagatg ggtgcccatc tcttctttga tttgcccttt attgcctaac cagccaaaac
     2221 cagtcggcac tacggaattt ttaccaaaat caaagatgcg gtcggtttcc tgttccagcc
     2281 aacggttgtg gcttagggtg ttaaaccatt tcattccttt tatcctcatc ttagcggcgt
     2341 ttagccatca tttcgtcgac gatatcgcca agttgttgta atttcggtgc gcaaacgtca
     2401 cggagcatca gctcgttgtc tggtaaaccg acgaccgatg cccagactgc acggcctgcc
     2461 aggaatcccg atgcgcccgc cgtcattgcc acgcgtacgg cacgcgggaa cagtttttcg
     2521 tcgacactgg aagagaggat cacccatggc atattgatat ggtcattcag acgttgtgaa
     2581 gcacagagaa gctcttgttg cggacctttg ccataaagcg gcatttcaac tttgtagagg
     2641 tcagcgccac tgtcgcccag ctctttggcg gcatcgatga tcgcttgttc gcgatcgaat
     2701 ttatcgccac gacgcggtgg gcggacgact ggctcaatga tgcttaccag accgtgtgag
     2761 tggcacagtt cgttgaactc tttcaccata tccagacgtt gctgcgcatc ttcatcacta
     2821 cgccacagca ccagcagttt taaggctttg ccaccgtctt gtttgatctg tagcggattg
     2881 attttgcggt caataaccac gctatcgacc ggaataccgt tgccaggaat gaactcatcg
     2941 gcggcgacaa tcatggcgca acttttggca atcgcgtttt gctcaaccac ctggcggtag
     3001 cagaattgtt gatctaccag aatcgccgag gcataaggcg agagggcctt tgcagcgtta
     3061 actttgaaat cagttaaaac gctatcggct accggagcgg gtgccccagc cgcggcaaac
     3121 atcatgcgca tggcttcgcg ctgatcgacc gccagcatgg caaaaccgcc cgatgcgcgc
     3181 gtaatgtcgt tgatggtgta cttattcatt catttattcc tttatccagt cgttacattt
     3241 ttactttggc agtcatcccg gcactgacac ggacctgttc cagaatggcg gaccagtcct
     3301 ggcgaccgcg acccgctgcg cgcgcctggc tataaacctc ccgtgaggcg gcccccagcg
     3361 gcattggcac atgcagctgg ttggcgacat caagggcgat gccaagatcc ttatgggcaa
     3421 gatcgatcat gaaggcggga gaaagatctc cgctgaggac tttgtttggc caggaagtgg
     3481 tgaagtgacc tttaccggcg gcggtaccgc tcatcacttt gacggcaaca tcgaagggaa
     3541 gattcagggc ttcgcacaaa acggcggctt ctgccgaaag cgcattgagc gcgatgctca
     3601 tgtagttgtt gatgagctta acgcggatcc ccatgcccgg accgccagcg ttgatcaact
     3661 cactgcccat cgccatcagg atcggtgtgg cacgttcaac ttgttcagcg gtgccgccag
     3721 ccagcagtaa cagagtaccg gtaatggcat ttgcagaagt acggcctacc ggaacatcca
     3781 tcatgctgaa gcctttggct tgcatatcgg caatcaattt atcggtttgc agcggatgga
     3841 tggtggacat atcaatgacc agcgcatcgg tagataagcc ttcgcaaacg ccgttttcac
     3901 cgaacaacac gttgcgcacc agatcgccat tcggcaacat ggtaatgata aattcggcat
     3961 ctttagcggc ctgcgccggg ttggcggcgg gagtcgcacc tttgtctacc agatgccgca
     4021 cagcttcggc gttcacatca aagacgcgaa gttgatgccc ttgctgcaat aaattgctcg
     4081 ccattggcga acccatttgt cctaaaccga taaacgcgat tgctgccata accctctcct
     4141 gaatacagtg atgtcacttt ttgtcattta tgacatgctt tgcttgtctg tttttgatcg
     4201 aatttgtaat ttatcgtcaa aaaattgaca gccgtcactt tttaaacaat tggtgaaatt
     4261 aaaatgaacg catcccaaaa tgtttaagga atgaccatga ttcgtgttgc ttgtgtaggt
     4321 ataaccgtga tggatcgcat ctattacgtg gaagggttac cgacggagag cggtaaatac
     4381 gtggcgagaa attatacgga agttggtggc gggccagcgg cgacggcagc ggttgcggcg
     4441 gcaaggctgg gggcgcaggt cgattttatt ggtcgcgtag gtgatgacga cgcgggcaac
     4501 agcctgctgg cagagctgga atcctggggc gttaacaccc gttacaccaa acggtataac
     4561 caggcgaaat cttcgcaatc cgccatcatg gtggatacca aaggcgagcg gataatcatt
     4621 aactacccca gcccggatct gctgcctgac gcagagtggt tggaggaaat tgatttctct
     4681 cagtgggatg ttgtgctggc agatgtacgc tggcacgacg gcgctaaaaa agccttcacc
     4741 ctggcccgtc aggcgggtgt gatgaccgtt ctggacgggg acattacgcc gcaggatatc
     4801 agtgagctgg tggcattaag cgatcacgcg gccttttcag aaccgggtct ggcgcgctta
     4861 acgggcgtga aagagatggc cagtgcgcta aaacaggcac aaacgctcac aaatggtcat
     4921 gtctatgtga cccagggtag cgcaggctgc gactggctgg aaaatggtgg gcgtcagcat
     4981 caaccggcct tcaaagttga tgtggtagat accacaggtg cgggagatgt ttttcacggc
     5041 gctttggcgg tggcgctggc aacaagtggg gatttagcgg agtcagtccg cttcgccagc
     5101 ggtgtagcgg cgttaaaatg cacacgtccc ggtggacgag ccgggatccc tgactgtgat
     5161 caaacccgat cttttttgtc actttttgta taaaatgcca gggtgatggt ttttcgagga
     5221 attttcatga gccttaccga actaaccggt aacccgcggc acgaccaact cctcatgctg
     5281 atcgccgagc gtgggtatat gaatattgat gagctggcaa atctgctgga tgtctccacg
     5341 cagacggtcc gccgggatat tcgtaaatta agcgagcaag gcctgattac gcgccatcac
     5401 ggtggcgcgg gtcgggcttc cagcgtcgtt aatacggcgt tcgagcagcg tgaggtttcg
     5461 caaaccgaga aaaaaaaagc gattgccgaa gcggtggcag actatattcc tgatggttca
     5521 acaatattta tcaccattgg tacgactgtt gagcatgttg cccgggcgtt acttaaccat
     5581 aatcatttgc ggataatcac caacagcctg cgtgtggcgc atattcttta ccacaacccg
     5641 cgctttgaag tgatggtgcc cggcggtacg ttgcgctctc ataatagcgg gatcattggc
     5701 ccttcagcgg cgtcctttgt ggctgatttt cgtgctgatt atctggtaac aagcgttggg
     5761 gcgattgaga gcgatggcgc gttgatggag tttgatgtaa acgaagctaa cgtggtgaaa
     5821 acgatgatgg cgcacgcgag aaatattctg ctggtcgccg atcacactaa gtatcatgct
     5881 tcggcggcgg ttgaaattgg taacgtggca caggtcactg cgctctttac cgacgagctg
     5941 ccgcccgctg cgctaaaatc acgcttacaa gacagccaaa ttgaaatcat tcttccccag
     6001 gaagacgcgt agatttttgt gaccgttaac ctggcttcat accttgccac atagccaaac
     6061 ccatcctttc ccgctacagt taatttcttg tggcgcgaaa ggaggcaaaa atgctctata
     6121 tctttgattt aggtaatgtg attgtcgata tcgactttaa ccgtgtgctg ggagcctgga
     6181 gcgatttaac gcgtattccg ctggcatcgc ttaagaagag ttttcatatg ggggaggcgt
     6241 ttcatcagca tgagcgtggg gaaattagcg acgaagcgtt cgcagaggcg ctgtgtcatg
     6301 agatggctct accgctaagc tacgagcagt tctctcacgg ctggcaggcg gtgtttgttg
     6361 cgctgcgccc ggaagtgatc gccatcatgc ataaactgcg tgagcagggg catcgcgtgg
     6421 tggtgctttc caataccaac cgcctgcata ccaccttctg gccggaagaa tacccggaaa
     6481 ttcgtgatgc tgctgaccat atctatctgt cgcaagatct ggggatgcgc aaacctgaag
     6541 cacgaattta ccagcatgtt ttgcaggcgg aaggtttttc acccagcgat acggtctttt
     6601 tcgacgataa cgccgataat atagaaggag ctaatcagct aggcattacc agtattctgg
     6661 tgaaagataa aaccaccatc ccggactatt tcgcgaaggt gttatgctaa aaaccattca
     6721 ggacaaagcc aggcaccgta cccgtccact atgggcctgg ctaaaactac tctggcaacg
     6781 tattgatgag gacaacatga caaccctggc aggtaacctt gcctatgtgt cgttgctctc
     6841 attagtgccg ctggttgccg ttgtttttgc gcttttcgcc gcttttccca tgttttccga
     6901 cgtcagcatt cagttacgtc actttatttt tgccaacttt ctgcctgcta ctggcgatgt
     6961 tattcagcgg tatatcgaac aatttgttgc taattccaac aagatgaccg ccgttggggc
     7021 gtgcgggctg atcgtcaccg cgttattgtt gatgtactcc atcgatagcg cgttgaatac
     7081 catctggcgc agtaaacgag cgcgaccaaa aatttactcg ttcgccgtgt actggatgat
     7141 tttaacgctg gggccgctgc tggcaggggc cagtctggcg atcagttcct atttgctctc
     7201 cctgcgctgg gcgagcgatc tcaatactgt catcgacaac gtgctgcgta tttttccgtt
     7261 gctgttgtcg tggatctcct tctggttgct gtacagcatt gttcctacca tccgcgtacc
     7321 taaccgcgac gcgattgtcg gcgcgtttgt cgccgcactc ctgttcgaag caggaaagaa
     7381 aggtttcgcg ctttatatca ccatgttccc gtcatatcag ctcatttacg gtgtgctggc
     7441 ggtgatcccc attctctttg tttgggtcta ctggacatgg tgtatcgtct tgcttggcgc
     7501 ggaaattact gtcactctcg gggaataccg caaactaaaa caagcagctg aacaagaaga
     7561 agacgacgaa ccatgattgc attaattcaa cgcgtaaccc gtgccagcgt caccgtggag
     7621 ggagaagtga cgggcgaaat tggcgcggga cttttggtgt tattgggtgt cgaaaaggat
     7681 gacgacgaac agaaagcaaa ccgtctgtgc gagcgtgtgc tcggctaccg catctttagc
     7741 gatgccgaag gcaagatgaa tctcaacgtg caacaggcgg gcggcagtgt actggtggtt
     7801 tcccagttta ccctcgccgc agataccgaa cgggggatgc gcccaagttt ctccaaaggc
     7861 gcatcaccgg atcgcgcaga ggcgttatat gactatttcg tcgaacgctg ccgccagcaa
     7921 gagatgaaca cgcaaacagg acgcttcgct gccgatatgc aggtatcgct ggtcaatgat
     7981 ggccccgtga cattctggtt gcaggtatga gccagcttcc agggttgtca cgggaaacaa
     8041 gagagagtat cgctatgtat caccttcagg ttccacaaac agaagaagag ttagagcgtt
     8101 actatcactt tcgctgggaa atgttgcgta agcccctgca tcaaccaaaa ggttcggaac
     8161 gcgacgcgtg ggatgcgatg gcgcatcacc agatggtcgt cgacgagcag ggtaatctgg
     8221 tggcggtagg ccgattgtat attaatgccg acaatgaagc gtccattcgc tttatggtcg
     8281 ttcacccgga cgtgcaggac aaagggttag gaacgctgat ggcgatgacc ctggagtcgg
     8341 tggcgcgtca ggaaggcgtt aagcgcgtga cctgtagcgc ccgtgaagac gcggtggagt
     8401 ttttcgccaa gctggggttt gttaatcagg gagaaatcac cacgccaacc accacgccga
     8461 ttcgccattt tttgatgatt aagcccgtcg ccactctgga tgatattttg catcgcggcg
     8521 actggtgcgc gcagctgcaa caggcgtggt acgaacatat cccgcttagt gaaaaaatgg
     8581 gcgtgcgtat tcaacaatat accgggcaaa aatttatcac caccatgccg gaaaccggca
     8641 atcagaatcc gcaccatacg ctgtttgccg ggagtttatt ctcgctggcg acgctcaccg
     8701 gttgggggct tatctggctg atgctgcgtg aacgccacct cggcggaacg attattctgg
     8761 cggatgcgca tatccgctac agcaaaccga ttagcggtaa accccatgcg gtagccgacc
     8821 ttggtgcctt aagcggcgat ctcgaccgtc tggcgcgcgg acgaaaagca cgggtgcaga
     8881 tgcaggtcga aatctttggc gacgagacgc cgggtgcagt gtttgaaggc acgtatatcg
     8941 ttctgcccgc gaagccattt ggcccgtatg aagagggcgg gaacgaagaa gagtagctta
     9001 tggagagggg cggtgctgcc tctctcattc attccggtca tttacatcag tccctgacta
     9061 ttccggttat attgaataag aactttataa atttttattc ttcgttggca ccatacgtgt
     9121 cttgctacat cactgttcag gagcatttaa atattatttg tagagctgag tggcaattat
     9181 tgcagataat gcttaactca tcatattagt tcaggtttga gcgatttttg atagtcaaaa
     9241 ccccctctgg tgccatactt ttttcataaa tatttttaaa atctatagct cacaaatatt
     9301 atgcaaagat aatgggctgg catacattgt cagtatcatt catttactgc tctgtatgtt
     9361 atgaatcatt atgttgtatg tttgctctta aaccgtggga aggaggctat aatggttgcc
     9421 aatattaatc tcattaaaca agaaagttat tctgttgtta atttagaaaa acaactaatg
     9481 agtaataaat caccgaagca aacgctatta aatc
//
LOCUS       DAZOYF010000149         9513 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZOYF010000149 DAZOYF010000000
VERSION     DAZOYF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.150"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..276
                     /locus_tag="ACHUOF_003341"
     CDS             <1..276
                     /locus_tag="ACHUOF_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR0612392.1"
                     /translation="PAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIV
                     VMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            432..1967
                     /gene="gadC"
                     /locus_tag="ACHUOF_003342"
     CDS             432..1967
                     /gene="gadC"
                     /locus_tag="ACHUOF_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR0612393.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2098..3417
                     /locus_tag="ACHUOF_003343"
     CDS             2098..3417
                     /locus_tag="ACHUOF_003343"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR0612394.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3794..4864
                     /gene="dosC"
                     /locus_tag="ACHUOF_003344"
                     /pseudo
     CDS             3794..4864
                     /gene="dosC"
                     /locus_tag="ACHUOF_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            4864..5481
                     /locus_tag="ACHUOF_003345"
                     /pseudo
     CDS             4864..5481
                     /locus_tag="ACHUOF_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5739..6071
                     /gene="ddpX"
                     /locus_tag="ACHUOF_003346"
                     /pseudo
     CDS             5739..6071
                     /gene="ddpX"
                     /locus_tag="ACHUOF_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            6085..6981
                     /gene="ddpC"
                     /locus_tag="ACHUOF_003347"
     CDS             6085..6981
                     /gene="ddpC"
                     /locus_tag="ACHUOF_003347"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR0612395.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6978..7952
                     /locus_tag="ACHUOF_003348"
     CDS             6978..7952
                     /locus_tag="ACHUOF_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0612396.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7945..8871
                     /locus_tag="ACHUOF_003349"
                     /pseudo
     CDS             7945..8871
                     /locus_tag="ACHUOF_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(8927..9358)
                     /gene="osmC"
                     /locus_tag="ACHUOF_003350"
     CDS             complement(8927..9358)
                     /gene="osmC"
                     /locus_tag="ACHUOF_003350"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR0612397.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2321 a         2331 c         2389 g         2472 t
ORIGIN      
        1 ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct gtacgacctc
       61 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
      121 accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
      181 ctgttgctgg aagactacaa agcctccctg aaatatctca gcgatcaccc gaaactgcag
      241 ggtattgccc agcagaacag ctttaaacat acctgataac gtttaacggt aacggtgtcc
      301 cgaaacgaac ccgtttcggg ataatttcca aagtctgttc actggcatta gcaacggaaa
      361 atattgttct gaatacgctt cagaacaaaa caggtgcggt tccgacagga ataccgtttt
      421 agggggataa tatggctaca tcagtacaga caggtaaagc taagcagctc acattactcg
      481 gattctttgc cataacggca tcgatggtaa tggctgttta tgaataccct accttcgcaa
      541 catcgggctt ttcattagtc ttcttcctgc tattaggcgg gattttatgg tttattcccg
      601 tgggactttg tgctgcggaa atggccaccg tcgacggctg ggaagaaggt ggtgtcttcg
      661 cctgggtatc aaatactctg ggtccgagat ggggatttgc agcgatctca tttggctatc
      721 tgcaaatcgc cattggtttt attccgatgc tctatttcgt gttaggggca ctctcctaca
      781 tcctgaaatg gccagcgctg aatgaagacc ccattaccaa aactattgca gcactcatca
      841 ttctttgggc gctggcatta acgcagtttg gtggcacgaa atacacggcg cgaattgcta
      901 aagttggctt cttcgccggt atcctgttac ctgcatttat tttgatcgca ttagcggcta
      961 tttacctgca ctccggtgcc cccgttgcta tcgaaatgga ttcgaagacc ttcttccctg
     1021 acttctctaa agtgggcaca ctggttgtat ttgttgcctt cattttgagt tatatgggcg
     1081 tagaagcttc cgcaactcac gtcaatgaaa tgagcaaccc cgggcgcgac tatccactgg
     1141 ctatgttact gctgatggtg gcggcaatct gcttaagctc tgttggcggt ttgtctattg
     1201 cgatggtcat tccgggtaat gaaatcaacc tctccgcagg ggtaatgcaa acctttaccg
     1261 ttctgatgtc ccatgtggca ccggaaattg agtggacggt tcgcgtgatc tccgcactgc
     1321 tgttgctggg tgttctggcg gaaatcgcct cctggattgt tggtccttct cgcgggatgt
     1381 atgtcacagc gcagaaaaac ctgctgccag cggcattcgc taaaatgaac aaaaatggcg
     1441 taccggtaac gctggtcatt tcgcagctgg tgattacttc tatcgcgttg atcatcctca
     1501 ccaataccgg tggcggtaac aacatgtcct tcctgatcgc actggcgctg acggtggtga
     1561 tttatctgtg tgcttatttc atgctgttta ttggctacat tgtgttggtt cttaaacatc
     1621 ctgacttaaa acgcacattt aatatccctg gtggtaaagg ggtgaaactg gtcgtggcaa
     1681 ttgtcggtct gctgacttca attatggcgt ttattgtttc cttcctgccg ccggataaca
     1741 tccagggtga ttctaccgat atgtatgttg aattactggt tgttagtttc ctggtggtac
     1801 ttgccctgcc ctttattctc tatgctgttc atgatcgtaa aggcaaagcg aataccggcg
     1861 tcactctgga gccaatcaac agtcagaacg caccaaaagg tcacttcttc ctgcacccgc
     1921 gtgcacgttc accacactat attgtgatga atgacaagaa acactaacca tgtctgaatg
     1981 gttataagac aagggagcga taattcatcg ctcccttttt cgtgcttgct gtcgtattga
     2041 cttaaccggg ataagtacga gaatgagcgc acatctgttt accggaaacc agcacatatg
     2101 gatatctgct cccgaaacaa gaaattaacg attagaagac cagcgatact ggttgcattg
     2161 gcacttttac tgtgtagttg taaaagcacg cctccagagt ccatggtgac accaccagca
     2221 ggttcaaagc caccagccac gacgcaacaa tcgtcacaac cgatgcgtgg catctggctg
     2281 gccacggttt ctcgcctcga ctggccaccg gtttcctcgg ttaacattag taatcccacc
     2341 agccgagccc gtgtacaaca acaggcgatg atcgacaaac tggatcatct ccaacgtctc
     2401 ggcataaaca cggtcttttt ccaggtcaag ccggacggta ccgccctgtg gccatcgaaa
     2461 attttgccgt ggtccgatct tatgaccggt aagattggtg aaaatccggg ttacgatccg
     2521 ctgcaattca tgctcgacga agcccacaag cgtgggatga aagtacacgc ctggtttaac
     2581 ccctatcgcg tatcggttaa tacgaagccc ggtactatca gggaactgaa tagcactctg
     2641 tctcaacaac cggcgagcgt ctatgtgcaa caccgtgact ggatcagaac gtctggcgat
     2701 cgctttgtcc tcgacccggg catccctgag gttcaggact ggatcacatc aatagtcgct
     2761 gaagtggttt cccgctatcc ggtagatggc gtgcagtttg acgactattt ctatactgaa
     2821 tcaccgggtt cacggctaaa tgataacgaa acgtaccgta aatacggagg cgcatttgcg
     2881 tcaaaagcag actggcggcg caacaatact cagcagttaa ttgcaaaggt atcacacacc
     2941 attaaaagca ttaagccggg agtcgaattt ggcgttagcc cggcaggcgt gtggcgtaac
     3001 cgatcacacg atccgctcgg ttccgatacc cgaggcgcgg cagcctatga cgaatcctac
     3061 gcagacaccc gtcgatgggt ggaacaagga ttgctggatt acattgctcc ccaaatttac
     3121 tggccgttct cacggagtgc cgcgcgttat gacgtgttgg caaaatggtg ggcggatgtc
     3181 gttaaaccaa ccaggacccg cctgtatatc ggtatcgcct tctataaagt gggtgaacct
     3241 tcaaagatag agccagactg gatgattaac ggcggcgtac cggaactgaa aaagcagctc
     3301 gatcttaacg atgcggtacc agaaattagc ggcaccatct tgttccgtga ggactatctg
     3361 aataaaccgc agactcaaca agcggtcagc tatctgcaaa gtcgttgggg cagttaaact
     3421 accatgctta ctttgtaaca agccggtgct ttacccaccg gcttgttaca ccttgtgaaa
     3481 tatcccacta gtaatcatgc ttacataagt caaattaacc acgcaattaa ccacacttag
     3541 ttaaacagct ataaatccct aataaaaatg tatgaatatg aaaaaccata cgatattcgc
     3601 tgtcaccgaa aaataattac gatgggctat actcttctga agaacagtag cgtagattga
     3661 aactgtctta agtcgcttat ttcttttcag tatatcttca tatttcagga gaatatggtg
     3721 cagttagtcg atctcgcacg ttgcgtttcg ttttgattcc gtctgatttc ccagccttat
     3781 aagggtgtga attatggaga tgtattttaa aagaatgaaa gatgagtgga ccggacttgt
     3841 cgaacaggca gatcctctca ttcgtgctaa agccgcagaa attgcccttg cgcatgctca
     3901 ttatctgagt atcgagtttt atcgaattgt ccgcatcgac ccgcatgccg aagaattctt
     3961 gagtaatgaa caagttgagc ggcagttgaa aagtgcgatg gaacgctgga ttattaacgt
     4021 gctttctgcc caggttgacg atgtcgaaag gctaatacaa atccagcata ccgtcgcgga
     4081 agtgcatgcc cgcataggaa ttccggtaga aattgtcgag atggggtttc gggtgctgaa
     4141 aaaaatcctc tatccggtaa tcttctcttc ggattattcc gccgcagaaa aacttcaggt
     4201 ctaccatttc tcgattaaca gtattgatat cgcgatggaa gtgatgaccc gcgcgtttac
     4261 ctttagtgac agtagtgcct caaaggaaga tgaaaactat cgtatcttct cgttactgga
     4321 aaacgccgaa gaagaaaaag aacggcaaat agcctcaata ctttcatggg aaatagatat
     4381 tatctataaa gtcctgctgg attctgattt aggcagcagt ttgcctttaa gccaggctga
     4441 ttttggcctg tggtttaacc ataaaggtcg acattatttt agcggcattg ccgaagtggg
     4501 ccatatctcc cgtctgattc aggatttcga cggtattttc aatcaaacca tgcgtaacac
     4561 caggattttg aataacagaa gtttgcgggt gaaatttcta ttacagataa gaaataccgt
     4621 atcgcagatt attaccttgc tgcgtgaatt gtttgaagaa gtatcgcgcc acgaagtcgg
     4681 tatggatgta ctgacgaaat tacttaaccg ccgtttcctg ccgactatct tcaaacgcga
     4741 aattgcccat gccaaccgga ccggtacacc gctgtcagtg ctgattattg acgttgataa
     4801 attcaaagag atcaacgata cgtggggcca taacactggc gatgaaattc tgcgtaaagt
     4861 ctcattcctc tcgcagaaga gattggtgaa atcgaaaata ttgggcgctg ggtcatcgcg
     4921 gaagcttgcc gtcagttagc agaatggcgt agccagaata ttcatatccc ggcgttatcc
     4981 gtgaacttgt cggcgctgca ctttcgcagt aatcagctgc ctaatcaggt gtctgatgca
     5041 atgcaagcct ggggtattga cggccaccag ctgacggtgg aaatcacgga aagcatgatg
     5101 atggaacacg ataccgaaat ctttaagcgc attcagatcc tgcgtgatat gggcgtgggt
     5161 ttatcggtag atgattttgg cacgggcttt tccggattat cccgcttagt cagtcttccg
     5221 gtaacggaaa tcaaaattga caaaagcttt gtcgatcgtt gtctgacgga aaaacgcatc
     5281 ctggccttac ttgaagccat taccagcatt gggcaaagcc tcaatttaac cgtcgtggcg
     5341 gaaggggtcg aaaccaaaga gcaatttgag atgctacgta agatccactg tcgcgttatt
     5401 cagggatatt tcttttcccg cccccttccc gccgaagaaa ttccaggctg gatgagcagc
     5461 gtgttaccgc tgaaaatctg acaaattcct ctcgcccgca ctcgcgggtt ttcctttaag
     5521 gtgacactgt cacttaaata ctgtgatttc agctacgatt ccgggagatt ccttccttaa
     5581 ctttccctct tgcaccaaaa ctgcactacg ctgcacaaat gttgatcaaa agttaaaaaa
     5641 tataacttta agccagcatt ctggcgctta tcccggcatg gcatgagatc tgcataagcg
     5701 gaaagcgcag caatttttgt cttatacagg aaccgtttat gtcggatacc accgaactgg
     5761 ttgatttagc cgtgatcttc cctgatctgg agatcgaatt gaaatatgcc tgcgttgata
     5821 acatcacagg taaagctatt tatcagcaag cgcgttgtct gctacataaa gatgcgatta
     5881 ccgcgctggc gaaaagtatc agtatcgccc agctgtcagg cttacaactg gtgatttacg
     5941 atgcgtatcg cccacaacaa gcacaggcga tgttgtggca agcctgccca gacccgcaat
     6001 atgttgttga tgtgacggtc ggttctaatc acagccgtgg cacggcgctc gacctgacgc
     6061 ttcgtgatga cgtggaggtg gtgaatgatg ctaagcgagg aaacgtccgc cgtacgccca
     6121 caaaaacaaa cgcgatttaa cggtgcaaaa ctggtatgga tgctgaaagg cagtccgctc
     6181 accgtgaccg gcgcagtcat cattgtatta atgcgactga tgatgatttt ttcaccgtgg
     6241 ctggcgacgc atgatcccaa cgccattgat ttaaccgccc gccttttgcc gccttctgcg
     6301 gcgcactggt ttggcaccga tgaagtggga cgcgatctgt ttagccgcgt actggtcggc
     6361 agtcagcaat caattctcgc cggattagtg gtggtcgcca ttgcgggtat gattggttcg
     6421 ctactcggat gtctatccgg tgtgcttggc ggacgcgcag acgccatcat catgcgcatc
     6481 atggacatta tgctgtcgat tccttcgttg gtactgacaa tggcactggc tgccgctctc
     6541 gggccgagtt tatttaacgc catgctggcg attgctattg tgcgaattcc cttttatgtg
     6601 cgcttggcgc gggggcaagc attagttgta cgccagtata cctatgttca ggcggcgaaa
     6661 acctttggcg cgtctcgttg gcatctgatc aactggcata ttttacgtaa ctccctaccg
     6721 ccgctgatcg tgcaggcatc gctggatatc ggtagcgcga ttttaatggc cgccacgttg
     6781 ggatttattg gcctgggtgc tcaacaaccg agtgctgaat ggggcgcgat ggtagcgaat
     6841 ggtcgcaact atgtactcga tcagtggtgg tattgcgcat ttccgggggc agcgattttg
     6901 cttacctccg tcgggtttaa tctctttggt gatggtattc gcgatctgct tgacccgaaa
     6961 gcaggaggaa agcagtcatg acccaacccg ttctggacat tcaacagctg catttgagtt
     7021 tccccggttt taacggtgat gttcacgcgc tcaacaatgt gtccttgcag attaaccgcg
     7081 gcgaaattgt cggtctggtg ggagaatccg gctcaggtaa atcagtcacc gcaatgctga
     7141 ttatgcgtct gctaccgacg ggcagttatt gcgtacatcg ggggcagatt tcactgctgg
     7201 gagaagatgt tcttaacgct cgggaaaaac agcttcgtca gtggcgcggc gcacgagtgg
     7261 cgatgatctt tcaggaaccg atgaccgccc ttaatccgac acgtcgaata ggtcttcaga
     7321 tgatggacgt aatccgccat catcaaccaa taagtcgtcg ggaagccaga gctaaagcga
     7381 ttgcactgct ggaagagatg caaatcccgg atgccgtgga agttatgtcg cgctatccgt
     7441 ttgagctttc aggtggtatg cgccagcggg taatgattgc gccggcattc tcctgcgagc
     7501 cgcaattgat tattgccggc gaaccgacga cggcgctgga cgtcacagta cagttgcagg
     7561 tactgcgttt gcttaaacat aaagcacgcg ccagtggaac tgcggtactg ttcatcagcc
     7621 atgatatggc cgtggtgtcg caactgtgcg atagcgttta cgtgatgtat gccggaagcg
     7681 taattgaaag cggcgggacg gcagacgtta tccatcatcc ccggcatccg tataccattg
     7741 gtttgctgca atgcgcacca gaacatggag taccacgcca gccattaccc gccattccag
     7801 ggacggtacc aaacctcacc catttgcctg acggctgcgc ttttcgcgat cgttgctatg
     7861 cggcaggtgc acagtgtgcg ctgacagcgt gtggtgacaa caaccagcac tgcacctgtt
     7921 ggtatccgca gcaggaggtc ataagtgtct gacacgttat taacgttacg cgacgtccat
     7981 atcaattttc cggcccgtaa aaactggctt ggcaaatcta cggaacatgt tcatgccatt
     8041 aatggtattg atttacagat ccgccgtggt gaaaccttag ggatcgtcgg cgagtcaggc
     8101 tgcggcaaaa gcaccctcgc acagctttta atgggcatgc tgcaaccgag ccacgggcag
     8161 tacatccgtt caggctcaca acgcattatg cagatggtgt ttcaggaccc gctctcttcg
     8221 cttaatccgc gcttaccggt gtggcgcatc atcacagaat cgctctggat agctaagcgt
     8281 agtagtgaac aacagcggcg agcgttggca gaggagttgg ctgtgcaggt gggtattcgt
     8341 ccggagtatc tcgaccgcct gcctcatgcg ttctccggcg ggcagcggca acgcatcgcc
     8401 attgccagag cactctcttc gcagcctgac gtgattgtgc ttgatgagcc aacctcggcg
     8461 ctggatatct ccgtgcaggc gcagatcctc aatttgctgg taacgctaca ggaaaatcac
     8521 gggctgactt atgtgctgat ttcacacaat gtctcggtga tacgtcatat gagcgatcgg
     8581 gtggcggtga tgtatctcgg gcagattgta gaactgggcg acgcgcaaca ggtgttgact
     8641 gcttcggcac atccatacac ccgattattg ctggattccc tccccgccat tgataaaccg
     8701 ctggaggaag aatgagcatt acgtaaaacg gatctgccgg gaaaccgcac gttgccgcaa
     8761 ggctgttttt tctacgaacg ttgccctctg gcaacccacg gatgtgaagt ccggcaatca
     8821 ttagcaataa gggaggacgg acgtgacctc cggtgctggc gggcgctgta gagatgctaa
     8881 caacgcatca ggcattcacg cgcctgacgc gtcatccggc aatgctttac gatttcaact
     8941 ggtaatccag cgtaatttcc gctttcagta cctgagagac cgggcatcct gcttttgctt
     9001 tctggatgat gccgtcaaag gtagaggcat caatacccgg caccgcaact tcactcttca
     9061 gtgcgatttt cgtaatcgca aaaccggcat ccactttatc cagcgacaca tcggcggtgg
     9121 tatcaattga tgttggcgtg aatcccgctt cccccagcat taatgaaagc gccattgaga
     9181 aacatgcggc atgcgctgcg ccaatcagtt cttcagggtt ggttcctttt tcgccttcaa
     9241 aacgcgtgtt aaatccatac ggctgttggt tcagcacgcc gctctcggtg gatactgttc
     9301 ccttcccgcg tttgatatcg ccttcccagt gtgcctgacc tttcttatgg attgtcattg
     9361 ttgctctcct gtgggcttaa aaaggaaatt atagactacg tgagaaacag cacgaggata
     9421 agcaggatat tccgaataaa atacggcgaa aatattactt ctgcacttaa attaaattcc
     9481 cacaatagat aaccgggaat atatatctca aga
//
LOCUS       DAZOYF010000150         9485 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZOYF010000150 DAZOYF010000000
VERSION     DAZOYF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.151"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..95
                     /locus_tag="ACHUOF_003351"
     CDS             <1..95
                     /locus_tag="ACHUOF_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFR0612398.1"
                     /translation="GASLPGVFARADRRPPPPALYHLFLLPCKM"
     gene            complement(65..256)
                     /locus_tag="ACHUOF_003352"
     CDS             complement(65..256)
                     /locus_tag="ACHUOF_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612399.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(253..675)
                     /locus_tag="ACHUOF_003353"
     CDS             complement(253..675)
                     /locus_tag="ACHUOF_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR0612400.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIARDWSLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREELEAMDVVLQALDGETMS"
     gene            complement(722..1147)
                     /locus_tag="ACHUOF_003354"
     CDS             complement(722..1147)
                     /locus_tag="ACHUOF_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR0612401.1"
                     /translation="MQYAKPVTLNVEECDRLFFLPYLFGQDFLYVEASVCALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            complement(1562..1705)
                     /locus_tag="ACHUOF_003355"
                     /pseudo
     CDS             complement(1562..1705)
                     /locus_tag="ACHUOF_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1806..2324)
                     /locus_tag="ACHUOF_003356"
     CDS             complement(1806..2324)
                     /locus_tag="ACHUOF_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR0612402.1"
                     /translation="MAPIACSWKRIPKFWNWIPASKIEKETRMYGTCETLCRALAAKY
                     PGDTPLMLVIWSPEEIQALADGMDISLSDHEIRTVLARLEDIPEDQRTESGISSGAAM
                     EIINNVRENRQVTVPAELLASLIQTAEQALWKREWAARDNGLAVPECVTRRQAVVNQA
                     RALLKNNTREND"
     gene            complement(2254..2475)
                     /locus_tag="ACHUOF_003357"
     CDS             complement(2254..2475)
                     /locus_tag="ACHUOF_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612403.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            complement(2476..3159)
                     /locus_tag="ACHUOF_003358"
     CDS             complement(2476..3159)
                     /locus_tag="ACHUOF_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR0612404.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLTAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(3235..3471)
                     /locus_tag="ACHUOF_003359"
     CDS             complement(3235..3471)
                     /locus_tag="ACHUOF_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612405.1"
                     /translation="MRRLTRRGINAACGQCPASGRARDGRFYRSRLASVTVYASPSPF
                     SDERPSSRFRGIALPSKRRRLRYSTVGLTRYRTR"
     gene            3363..3593
                     /locus_tag="ACHUOF_003360"
     CDS             3363..3593
                     /locus_tag="ACHUOF_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HFR0612406.1"
                     /translation="MSPTPAGSGKSARPVPGRTRDTDHRQRLCRAASAGAVKRGARRH
                     PLSEKKRAVARPSPSYFIRHNAPHCRLSTQVR"
     gene            complement(3544..4446)
                     /locus_tag="ACHUOF_003361"
     CDS             complement(3544..4446)
                     /locus_tag="ACHUOF_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR0612407.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEELPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIIDNVAHYGG"
     gene            4865..5113
                     /locus_tag="ACHUOF_003362"
     CDS             4865..5113
                     /locus_tag="ACHUOF_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0612408.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            5110..5547
                     /gene="umuD"
                     /locus_tag="ACHUOF_003363"
     CDS             5110..5547
                     /gene="umuD"
                     /locus_tag="ACHUOF_003363"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR0612409.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            5547..6299
                     /gene="umuC"
                     /locus_tag="ACHUOF_003364"
                     /pseudo
     CDS             5547..6299
                     /gene="umuC"
                     /locus_tag="ACHUOF_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
     gene            complement(6418..6918)
                     /locus_tag="ACHUOF_003365"
     CDS             complement(6418..6918)
                     /locus_tag="ACHUOF_003365"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0612410.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     gene            complement(7046..7885)
                     /gene="sul1"
                     /locus_tag="ACHUOF_003366"
     CDS             complement(7046..7885)
                     /gene="sul1"
                     /locus_tag="ACHUOF_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR0612411.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(7879..8226)
                     /locus_tag="ACHUOF_003367"
     CDS             complement(7879..8226)
                     /locus_tag="ACHUOF_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR0612412.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            complement(8410..8883)
                     /gene="dfrA5"
                     /locus_tag="ACHUOF_003368"
     CDS             complement(8410..8883)
                     /gene="dfrA5"
                     /locus_tag="ACHUOF_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA5"
                     /protein_id="HFR0612413.1"
                     /translation="MKVSLMAAKAKNGVIGCGPHIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSAWTADNDNVIVFPSIEEAMYGLAELTDHVIVSGGGEI
                     YRETLPMASTLHISTIDIEPEGDVFFPNIPNTFEVVFEQHFSSNINYCYQIWQKG"
     gene            9039..>9485
                     /gene="intI1"
                     /locus_tag="ACHUOF_003369"
     CDS             9039..>9485
                     /gene="intI1"
                     /locus_tag="ACHUOF_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR0612414.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISE"
BASE COUNT         2130 a         2747 c         2591 g         2017 t
ORIGIN      
        1 caggcgccag cctgccaggg gttttcgcgc gggctgacag gcggcctcca ccgcctgccc
       61 tgtatcacct gtttctgctg ccctgtaaga tgtagtgacc gcccaggaaa accgcctgct
      121 gattcgccac aaaatcgcag atttcatcaa caatctgcgc ggtggtgagc ggtttctgcc
      181 ccatttcacg acgccgcgca ttaatcacgg ccctgtaatt acgcagaatt tcgcgggttt
      241 ctgtgctgat attcatgaca tcgtctcccc gtccagcgcc tgcaacacaa cgtccattgc
      301 ttcgagttcc tccctgacga atgcgtcacc gtcgcccccg ttctcactcc cgacggcata
      361 caggcgcttc atttcactac ctgccagcgc catcactttt tccagcatca ccgccgggac
      421 tgtcacatga cggctggcgc gcttttcttc catcagttca ttgacaatat cggaaacaac
      481 tctgtcacat gcgccatata caaaagcatc gtccagccgc tgcatgacgg tttccagctc
      541 atcgtctgac aggctccagt cccgggctat gtgacgcaca tccccacggg tcagcactac
      601 ggcaaaaacg cattcactgt ccggcacgtc tgtatccagc acttcgcgga taatttcttt
      661 aacagtacag tacatgttct cgtctccttt acaggggcgg cattgccgcc ccgtccgtta
      721 atcagtccag cgccgcgtaa atcgcgttgc attcagggtg aaattcgatg tggctccaca
      781 actgcgcatc acgcatcctg tacaggtgtg tcaaccctgc atccccgctg tcgtggtaca
      841 gccacaactg gcggttaatc acaagggagg taaggatgat gcctgcggca tcagcactga
      901 ccgtgcggtc aaaccagttt tcaccgttca ccatgtggaa acggtcgcca tccggcatca
      961 tgtaaccgcc accgtccggc aggcggatga agtgccagaa tccgccttca tattccggca
     1021 tcattttttt cgccagcgcg cacacagacg cttcgacata cagaaaatcc tggccaaaca
     1081 ggtaaggcag aaaaaacaga cggtcgcact cttcaacatt cagagtgaca ggtttcgcat
     1141 actgcatggg atttcctccg ttgttcacat attccgcgac accgggcggt gccgtgcctc
     1201 catctttgcc gggcctgcag ggttgcaagg gcgcaaggga agccgcagcc tgacgcacac
     1261 ggctggcggg agttggaggg tgagaccgcg aaatctgagc gggtgaacga ccggaaaatc
     1321 acggcagacg ggaaggaggg accgggtgac cgcagcggtt caccttacct tgcaacccgc
     1381 ccgcagaagc ccggctacga tggaggcacg gcacagcccg cacacgacgc tgaggccaca
     1441 taactgaaaa cccacaaggc catgccccgc atggccgggt aaagcggagc ctgcggcgac
     1501 atccttttgc tctttccggg gaccggctgg aaaaggcgga gatttttccc cgccagcgtc
     1561 atcatttccg gattttcgcc agtgcatccg ccatgctgcg gatttcagtc cgggcttcca
     1621 ggaccacctc ccgcagaata tcccggtcaa accagcgacg ggacggttca tcaggtatac
     1681 gcagcacatc gcaggccagc cctgcgccag cagcaggtta cgggcgtggc gacaaatcag
     1741 tgcattcagc gtttcgttca tgattcatgg tctccctgaa tcgccgcccg caggcggcga
     1801 taacatcagt cgttttcgcg tgtgttgttt ttcagcaggg cgcgggcctg attaaccacc
     1861 gcctgtcggc gggtgacgca ttccgggacg gcgaggccgt tatcacgggc ggcccactca
     1921 cgtttccaca gtgcctgttc ggcggtctga atcagggacg ccagcagttc agcagggacg
     1981 gtcacctggc ggttttctct cacgttattg atgatctcca tcgccgcacc ggaagaaata
     2041 ccggattcag tccgctggtc ttccgggatg tcctccaggc gcgccaggac agtcctgatt
     2101 tcatgatcgg acagggaaat atccattccg tcagcgaggg cctgaatttc ttccggggac
     2161 cagataacca gcatcagcgg cgtatctccc ggatactttg ctgccagcgc ccggcagagc
     2221 gtttcgcatg ttccgtacat tctcgtctcc ttctcaatct ttgaagcagg aatccagttc
     2281 cagaatttcg gtatacgctt ccatgaacag gcgattggcg cgatacagcg tctggcgtgt
     2341 caggcgttca acaagcacat cggaatcaaa gcgacagcga tgctccatat cgttcagcct
     2401 ggcccgaagg tcacacatgg tgttggccac ctccgccacg tcggcgcgaa gtttttcgtg
     2461 tccggcatag ttcatttacg cagcctccgg ttcaaaccag ttatcattcg cggccccctg
     2521 ctgcatggcc cgttgcacgg cggtcagtcg ttgctgtccg gcacggtgat actgttcaag
     2581 cagctcgata ccgatatacc ggcgtccgga ctgaagagcg gccacgcagg ttgatccgct
     2641 gcctgcaaac gggtccagca caatcgcgtt cggatgtgtg aagctctcaa tcagtggttg
     2701 caggctggta acgggctttt ccgtcgggtg atggcgattg cccgaatatt tccagcccag
     2761 cacgtccggc agcgggtttt gtggcagacg tggacggcct tttgccagga tgtaggcaca
     2821 ttcgtggcga tagcccacat atgcggcctt cgatgtgtag tttttggtga agaccaggtg
     2881 accaacaacg ctgaatcccg catttttcca ggcagacata aaacgatcga cgcgattcca
     2941 gccatagaag ctcaccatta acgcgtcttt tttgagtacg cggtacatct cattacaggc
     3001 cggttgcagc cattcatcgg ttttatcgcc ggcaatggta cgcccctgac ggtcacgaaa
     3061 accgacgagg tacggcgggt cggtgaggat gaaatcaatg gcgttatcag ggatacgggc
     3121 cataacatcg atgcagttac cgaggacaaa acgggacata ccaaactcct tctcttacag
     3181 agggcaccgg gagggcaccc ctgtggtgcc cgttccccgc tgcctgtggg ctgttcaccg
     3241 cgtccggtaa cgggtcaggc cgaccgtgga ataccgcagc cgtcgtcttt ttgacggcaa
     3301 ggctatgccg cgaaagcggc ttgaagggcg ttcatcagaa aagggcgacg ggctggcgta
     3361 cactgtcacc gacgccagcc gggagcggta aaagcgcccg tcccgtgccc ggccggacgc
     3421 gggacactga ccacaggcag cgtttatgcc gcgccgcgtc agccggcgca gtaaaaagag
     3481 gagcacggcg acatcctttg tcggaaaaga aacgggcggt tgcccgcccg tctccgtcat
     3541 acttcatccg ccataatgcg ccacattgtc gattatccac gcaggtccgg tgacttccgg
     3601 cagctcatca tcatctgtcg gggaagacca ttccagttcc ccccacagca catcaacgag
     3661 ctctccgcgc tcatacaact gccagccaca gaaattgcag ccctgttcgg cgtaccagtg
     3721 ctccagcgtg cagccgaaac ggcggcttaa ttctgcaaca acgtcgcttt ccggctgaca
     3781 ccacggcgtg tcgaaatcta cctgaatgaa gttttccccc tgactgctga tattcaggtc
     3841 atacccacaa ggccacttca cgccataccg ttctgtatac cagtgatatg cagaaggaac
     3901 accgttcaga acaccgccgt taaagccatt cacttcaata tccaggcggg tcggcaaaac
     3961 cgtcagcagg tcaaacggca tggttttttc cggcagtacg ttgtcacaca gacggttcca
     4021 ccacaccgat acatcctcgt taccccagat gtcacaccag tcacctcttt ttgcggtgaa
     4081 atgcacagtg atagtttccc tgacatcatc cggtaactct tcccagcgac gctgtccagt
     4141 accgctctgc agccagagtt catgcagcat caggcagttt tgctcatcca gcagtacacc
     4201 gttctgcagg tgtgtcagcc agcgggtgaa cgcgatattt tccggtgata cagcaccacg
     4261 tccggcagcc gtcactccgg ggcactgctc agagcggata ttttcagtga tctgcagaag
     4321 tccggcactt ccggccagaa acagctgaat accttcattt gtggcgcggc gataaagcgg
     4381 tgtaactgca ccgctggcca gtcgtttaat ctcagcgatc tgtgccggtt caccagaaaa
     4441 atacatacga ttcgagcacc agtttggcat gggaattctc ctctcacatg cggcctccat
     4501 caggccgtgc cgcacctgtt accgggcgac cgcatacgaa tgcaggtgcg gccccgaagc
     4561 cgcagcgcgt ggaggaggaa cgacgaaacg tgaccgggtg aggaggccgg cgacagcgac
     4621 acacttgcat cggatgcagc ccggttaacg tgccggcacg gcctgggtaa ccaggtattt
     4681 tgtccacaga accgtgcaca aaatgctgtg gataagctgg acacagcagc ccacagcagg
     4741 catacagcca cacactcaga gaattgagcg ttttttagtc acaacaatga catttgactg
     4801 tgtgcggatt cagttattag aatactgtat aaacatacag cataaagaaa agggagatga
     4861 gaacatgatc cgcattgaaa ttcttttcga ccgccagagc acaaaaaacc tcaaatcagg
     4921 cacacttcag gcgctacaga atgaaatcga acaacgtctg aaaccacact acccggaaat
     4981 ctggctgcgt atagaccagg gcagcgcccc gtctgtatct gtcacaggag cccgcaacga
     5041 caaggataaa gaacgtatcc tgtctctgct ggaagaaatc tggcaggacg acagctggct
     5101 gcctgcagca tgagtaccgt ttatcaccgt ccggctgacc cttcaggcga tgacagttac
     5161 gtgcgaccgt tgtttgccga tcgttgccag gccggttttc cttcgcctgc cactgattat
     5221 gctgagcagg aactggatct gaacagctat tgcatcagca gacctgcagc caccttcttt
     5281 ctgcgcgcca gcggtgaatc gatgaaccag gctggcgtgc agaatggtga tctgctggta
     5341 gtggacaggg ccgaaaaacc acaacacggg gacatcgtta tcgctgagat cgacggtgag
     5401 ttcaccgtca aacgactgct gttgcgccca cgcccggcac tggagccggt ttcagacagc
     5461 ccggagttcc gcacactgta tccggaaaac atctgtattt ttggtgttgt cactcacgtg
     5521 atacacagga cgcgggagtt acgctgatgt ttgcactggc tgatatcaac agtttctacg
     5581 cctcatgtga aaaagttttc cgcccggacc ttcgcaacga accggtcatc gtactcagca
     5641 ataacgatgg ctgtgtgatc gcgcgcagcc cggaggcaaa agcccttggc atcagaatgg
     5701 ggcagccctg gtttcaggtg agacaaatgc gcctggagaa gaaaatacat gtattttcca
     5761 gcaattatgc gctgtaccac agcatgagcc aacgggttat ggctgttctg gagtcgcttt
     5821 ctcccgcagt tgagccctac tcaattgatg aaatgttcat tgatttgcgg gggataaatc
     5881 attgcatctc tccggagatt tttggtcatc agctcaggga acaggtaaag agctggacag
     5941 gactcaccat gggggtgggc attgcgccta caaaaacgct ggctaaaagt gcccagtggg
     6001 caacaaagca atggccacag ttttccggtg tggtcgcgct gacggcagaa aaccgtaatc
     6061 ggatcttgaa gctactgggg ttgcagccag ttggtgaggt ctggggagta ggacgcagac
     6121 tgacggaaaa gctgaatgcg ctgggtatta acacagcact gcagctggcg caggctaaca
     6181 cggcattcat ccggaaaaac ttcagcgtca ttcttgagcg tacggtacgc gaactcaacg
     6241 gcgagtcctg catatctctg gaagaagcac cgccggcaaa acagcagatt gtctgtagtg
     6301 gaagtggagg atatcgacaa cctctcgcgc aaccaagaca tcgcggtcgg actgcaagtg
     6361 atcttgaagc cacgggcccg tcccaccccg acatggacct cgatgcccga acggacgtta
     6421 gatttcgagt tctaggcgtt ctgcgatgaa ggttggatcc cagccgggat tgaaagtgtc
     6481 gacgtgggtg aatccgagcc gctcgtatag gccacgcagg ttcgggtggc agtcgagccg
     6541 cagcttggcg cacccctgcg ttcgcgcggc atggcggcaa gcctcgatca gcgcggagct
     6601 gacaccccgg cccgcatgtg tccgtcgcac cgcgagcttg tgcagatatg cggcctcccc
     6661 cttgagggcg tcgggccaga actcgggatc ctcggccgac aaggtgcaac agccgacgat
     6721 gccgtcgctg caactcgcga ctaggagctc ggatctcagg acgaaggtct ccgcgaatgt
     6781 ccggtcgatc cgcgcgacgt cccaggcggg cgttcccttg gcggacatcc acgccgcagc
     6841 gtcgtgcatc agccgcacaa cctcgtcgat atcacccgag caggcgaccc gaacgttcgg
     6901 aggctcctcg ctgtccattc gctcccctgg cgcggtatga accgccgcct catagtgcag
     6961 tttgatcctg acgagcccag catgtctgcg cccaccttcg cggaacctga ccagggtccg
     7021 ctagcgggcg gccggaaggt gaatgctagg catgatctaa ccctcggtct ctggcgtcgc
     7081 gactgcgaaa tttcgcgagg gtttccgaga aggtgattgc gcttcgcaga tctccaggcg
     7141 cgtgggtgcg gacgtagtca gcgccattgc cgatcgcgtg aagttccgcc gcaaggctcg
     7201 ctggacccag atcctttaca ggaaggccaa cggtggcgcc caagaaggat ttccgcgaca
     7261 ccgagaccaa tagcggaagc cccaacgccg acttcagctt ttgaaggttc gacagcacgt
     7321 gcagcgatgt ttccggtgcg gggctcaaga aaaatcccat ccccggatcg aggatgagcc
     7381 ggtcggcagc gaccccgctc cgtcgcaagg cggaaacccg cgcctcgaag aaccgcacaa
     7441 tctcgtcgag cgcgtcttcg ggtcgaaggt gaccggtgcg ggtggcgatg ccatcccgct
     7501 gcgctgagtg cataaccacc agcctgcagt ccgcctcagc aatatcggga tagagcgcag
     7561 ggtcaggaaa tccttggata tcgttcaggt agcccacgcc gcgcttgagc gcatagcgct
     7621 gggtttccgg ttggaagctg tcgattgaaa cacggtgcat ctgatcggac agggcgtcta
     7681 agagcggcgc aatacgtctg atctcatcgg ccggcgatac aggcctcgcg tccggatggc
     7741 tggcggccgg tccgacatcc acgacgtctg atccgactcg cagcatttcg atcgccgcgg
     7801 tgacagcgcc ggcggggtct agccgccggc tctcatcgaa gaaggagtcc tcggtgagat
     7861 tcagaatgcc gaacaccgtc accatggcgt cggcctccgc agcgacttcc acgatgggga
     7921 tcgggcgagc aaaaaggcag caattatgag ccccatacct acaaagcccc acgcatcaag
     7981 cttttgccca tgaagcaacc aggcaatggc tgtaattatg acgacgccga gtcccgacca
     8041 gactgcataa gcaacaccga cagggatgga tttcagaacc agagaaagaa aataaaatgc
     8101 gatgccataa ccgattatga caacggcgga aggggcaagc ttagtaaagc cctcgctaga
     8161 ttttaatgcg gatgttgcga ttacttcgcc aactattgcg ataacaagaa aaagccagcc
     8221 tttcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa
     8281 gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgccgt
     8341 tatcaattgc ggtaagaagc gtagcaagcg aagcgaacga agctttttac cgtcaattgc
     8401 atagctttgt taaccctttt gccaaatttg atagcaatag ttaatgtttg agctaaagtg
     8461 ttgctcaaaa acaacttcga aggtattggg aatattcgga aagaaaacat ctccttccgg
     8521 ctcaatatca atcgtcgata tatggagcgt agaggccatg ggcaatgttt ctctgtaaat
     8581 ctccccgcca ccagacacta taacgtgatc ggtgagttca gccagcccgt acatggcctc
     8641 ttcgatcgac gggaatacta ttacgttgtc attatcggcc gtccaggctg agcgagtaac
     8701 gaccgcgtat ttcctattag ggagtgctcc catagattcg aacgtcttgc ggcccaccaa
     8761 aagccactgg ttgtacgtca aggctttaaa gagtagctgc tctcctttcg cggaccaggg
     8821 tatgtgtgga ccgcaaccaa tcactccgtt tttcgctttt gcagccatta atgatacttt
     8881 cacaattttg gttccgggtt aactttgttt tagggcgact gccctgctgc gtaacatcgt
     8941 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
     9001 gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt
     9061 taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc
     9121 attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgccttc atccgtttcc
     9181 acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc
     9241 tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt
     9301 tcttctacgg caaggtgctg tgcacggatc tgccctggct tcaggagatc ggaagacctc
     9361 ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc atcctcggtt
     9421 ttctggaagg cgagcatcgt ttgttcgccc agcttctgta tggaacgggc atgcggatca
     9481 gtgag
//
LOCUS       DAZOYF010000151         9478 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZOYF010000151 DAZOYF010000000
VERSION     DAZOYF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.152"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..154
                     /locus_tag="ACHUOF_003370"
     CDS             <1..154
                     /locus_tag="ACHUOF_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HFR0612415.1"
                     /translation="EFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQIAEDLLK
                     ENPVND"
     gene            147..554
                     /gene="mxiM"
                     /locus_tag="ACHUOF_003371"
     CDS             147..554
                     /gene="mxiM"
                     /locus_tag="ACHUOF_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HFR0612416.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            538..966
                     /gene="mxiM"
                     /locus_tag="ACHUOF_003372"
     CDS             538..966
                     /gene="mxiM"
                     /locus_tag="ACHUOF_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HFR0612417.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            1096..1728
                     /gene="mxiE"
                     /locus_tag="ACHUOF_003373"
     CDS             1096..1728
                     /gene="mxiE"
                     /locus_tag="ACHUOF_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFR0612418.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            1715..3415
                     /gene="mxiD"
                     /locus_tag="ACHUOF_003374"
     CDS             1715..3415
                     /gene="mxiD"
                     /locus_tag="ACHUOF_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFR0612419.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            3434..4501
                     /gene="mxiC"
                     /locus_tag="ACHUOF_003375"
     CDS             3434..4501
                     /gene="mxiC"
                     /locus_tag="ACHUOF_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR0612420.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            4514..6574
                     /gene="mxiA"
                     /locus_tag="ACHUOF_003376"
                     /gene_synonym="virH"
     CDS             4514..6574
                     /gene="mxiA"
                     /locus_tag="ACHUOF_003376"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR0612421.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            6587..6988
                     /gene="spa15"
                     /locus_tag="ACHUOF_003377"
                     /gene_synonym="spaK"
     CDS             6587..6988
                     /gene="spa15"
                     /locus_tag="ACHUOF_003377"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFR0612422.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            6992..8284
                     /gene="spaL"
                     /locus_tag="ACHUOF_003378"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             6992..8284
                     /gene="spaL"
                     /locus_tag="ACHUOF_003378"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFR0612423.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            8365..8715
                     /gene="spa13"
                     /locus_tag="ACHUOF_003379"
                     /gene_synonym="spaM"
     CDS             8365..8715
                     /gene="spa13"
                     /locus_tag="ACHUOF_003379"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFR0612424.1"
                     /translation="MYRDVEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVK
                     KHIIQYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLI
                     LDELSQEDMKYGIR"
     gene            8702..>9478
                     /gene="spa32"
                     /locus_tag="ACHUOF_003380"
     CDS             8702..>9478
                     /gene="spa32"
                     /locus_tag="ACHUOF_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HFR0612425.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILK"
BASE COUNT         3189 a         1305 c         1889 g         3095 t
ORIGIN      
        1 ggagtttagt cctcaggatg taatatcagg tgttaaaata gaacttgcgg aaaaattaac
       61 aaaaaatgat aaaaaatatt tcaaagagtt agcacataag aaactgagac aaattgcaga
      121 agacttgtta aaagagaatc ctgttaatga ttaatcaaat aaatgcaagc aatgctctgc
      181 agcaaagatt aaatagtgaa gaagttgtta atttaaatga gagattatca tcttcgcaat
      241 catttgatga ggatataatc tatgaaataa tgcaatattt ctcacagagt gaattaaatt
      301 ccatagataa tgatgaactg cacaataaga ttgaacaatt attcaattca cgttttcctt
      361 atcttacagc tgcacaaaaa agttcacttc ttaataaact catagatgca aatcaatatg
      421 tcgatttgca tgagggattt tatgcatcat tgtccatata taacaatatt gatttctata
      481 ttaagactac aaccttcgat tcattaatta gtgtctttga agcagggaga gaggcagatg
      541 attcgacatg gtagtaataa gttgaaaata tttattttaa gtatattgct attaacactg
      601 agtgggtgtg ctttaaagtc atcatctaat tctgaaaaag aatggcatat tgttcctgta
      661 agtaaggatt atttttctat tccaaatgat ttattatggt cgtttaatac aaccaataaa
      721 agtataaatg tttactctaa atgtattagt ggtaaggcgg tttatagttt taatgcaggt
      781 aaattcatgg gcaactttaa tgttaaggaa gtagatgggt gcttcatgga tgcacaaaag
      841 atagctatag ataaactatt ttctatgctg aaagacgggg ttgttttaaa aggtaataag
      901 ataaatgata ccatccttat agagaaggat ggggaagtta aattaaaatt aattcgaggg
      961 atataattgt attgtgagta aatataaagg tctaaataca agtaatatgt tttacattta
     1021 ctctagtgga catgaaccag ttaacgttga gcttgtaaaa gataaagaac gtaacataat
     1081 tgagctggct ccagcatgga agggtttttt tttgtccgaa atcaaaatat aaaattcagt
     1141 gataatgtga actatcatta tcgctttaac attaatagtt gtgcaaaatt tcttgctttt
     1201 tgggactatt tcagtggtgc tctagtagag catagccatg ctgaaaaatg tattcatttc
     1261 tatcatgaga atgatcttcg ggatagttgt aataccgaat cgatgcttga taagttaatg
     1321 ttaagattca tcttttcatc tgaccagaat gtaagtaatg cactggctat gatacgaatg
     1381 actgagtcat atcatttggt tctttatctt ctgagaacta ttgagaaaga aaaggaggta
     1441 agaataaaga gtctgacaga gcattatggt gtgtcggagg cttattttag atctttatgt
     1501 agaaaagctt taggggcaaa agtcaaagaa caactgaaca cttggcggtt agtaaatggt
     1561 ttattagatg tatttttgca caatcaaaca atcacttcag ctgccatgaa taatgggtat
     1621 gcatcaacat cccatttttc aaatgagata aaaacacgac taggcttttc tgcaagagaa
     1681 ttatcgaata ttactttttt agtgaaaaaa ataaatgaaa aaatttaata ttaaatcttt
     1741 gactctcttg attgtattgt taccattgat agttaatgct aataatattg actctcattt
     1801 attagaacaa aatgacatag cgaaatatgt tgctcaaagt gatacggtag gatctttttt
     1861 tgaacgtttt tctgcgttat taaattatcc tattgttgtt agcaaacaag ctgcaaaaaa
     1921 gagaatatct ggggagtttg atttatctaa tccagaagaa atgcttgaaa aactgacatt
     1981 acttgtggga ctcatatggt ataaagatgg aaatgcatta tacatatatg actctggaga
     2041 attaataagc aaagttattt tgctggagaa tatatcactt aattatctta ttcagtatct
     2101 gaaggatgct aacctttatg atcacagata tcctataaga ggaaacatta gtgataaaac
     2161 attctatata tctggtcctc cggcattggt tgaactggta gctaatacag caactttgct
     2221 tgataagcag gtttcttcta ttgggacaga taaagttaat tttggggtta taaaattaaa
     2281 aaatacattt gttagtgaca gaacttataa tatgagaggg gaggatattg tcatcccagg
     2341 ggtggctact gttgtagaac gtttattaaa taatggtaag gctctgagta acagacaagc
     2401 ccaaaatgat ccgatgccac cctttaatat tacacagaaa gtatctgagg atagtaatga
     2461 cttttcattt agttcagtta ccaatagttc tatattagag gatgtatcac ttattgcata
     2521 tccagaaacc aattctatat tagtcaaagg taatgatcag caaatacaga ttattcgcga
     2581 tattatcact cagttagata tagccaaacg acatatagaa ttatcattat ggataatcga
     2641 tattgataaa tctgaattaa ataaccttgg tgttaattgg caaggaacgg cttcatttgg
     2701 tgattcgttt ggtgcgtcat ttaatatgag ctcatcagca tcgataagca ctttggacgg
     2761 caataagttt attgcttcag ttatggcatt aaatcagaaa aagaaagcaa atgtagtttc
     2821 aaggccggtg attttgacac aagagaatat tccggctata tttgataata acaggacttt
     2881 ttatgtgtca ttagttggtg agcgcaattc ttcgttggag catgttacgt atggaacatt
     2941 gataaatgtt atacctcgct tttcatctcg gggacaaatt gagatgtctt taacgataga
     3001 ggatggaaca ggaaatagtc agtcaaatta caattataat aatgaaaata catctgtcct
     3061 tccagaagtt ggaaggacca aaatcagtac aatagcgaga gttccacaag gcaaaagttt
     3121 gttgataggt ggttatacgc atgaaacgaa ctctaacgaa ataatatcta ttccttttct
     3181 ctcctctatt cctgtaatcg gtaatgtgtt caagtataaa acaagtaata tcagtaatat
     3241 tgtaagggtt tttctgattc aaccccgtga gataaaggag tcaagttatt ataatactgc
     3301 agaatataaa agcctcatat ctgaaagaga aatacaaaag actacacaaa taatcccatc
     3361 tgaaactact ttgctggaag acgaaaaatc attggtttca tacttaaatt actaactata
     3421 aagtaggtga tgtatgcttg atgttaaaaa tacaggagtt tttagctctg cattcattga
     3481 taagttgaat gcaatgacaa attcagatga tggagatgag actgctgatg cagagcttga
     3541 ttctggcttg gctaatagca agtatattga ctcatctgat gagatggctt ccgctctttc
     3601 gtcatttata aacagaagag accttgagaa actgaaagga acaaatagtg atagtcagga
     3661 acgtatttta gatggggaag aagatgaaat taatcacaag atttttgatt taaagagaac
     3721 gttaaaagat aaccttcctc tagatcggga ttttatagac agactaaaga gatattttaa
     3781 agatccaagt gatcaagtct tagcattaag ggaacttttg aatgaaaaag atcttactgc
     3841 tgaacaagtc gaattattaa ctaaaattat taatgagata atatcaggta gtgaaaaaag
     3901 tgttaatgct ggaataaatt cagctataca ggctaaatta tttggcaaca aaatgaaact
     3961 tgaaccacag cttttgcgtg catgttatcg tggttttatc atggggaaca tatcaacaac
     4021 agatcagtat atagaatggc ttggtaattt tggttttaat cacagacata caattgtgaa
     4081 ttttgtagag cagtcactga ttgtagacat ggattctgag aaaccgagct gtaatgctta
     4141 tgagtttggt tttgtgttat ctaaattaat tgcaattaag atgattagaa cttcagacgt
     4201 aatttttatg aagaaactgg aatcctcaag cttgctaaaa gatggcagtt taagtgcaga
     4261 gcagctattg ctaactttat tatatatttt tcaatatcca agtgaaagtg agcaaattct
     4321 tacttctgtt atagaagtat cacgagccag tcatgaggat tctgtagtgt atcaaacata
     4381 tctatcttct gttaatgaaa gtcctcatga tatatttaaa agtgaaagtg aaagagaaat
     4441 tgcgatcaat attctacgag agcttgtcac aagtgcatac aagaaagagc tttctagata
     4501 acaggagata aaagtgatcc agtcttttct taagcaagta agtactaagc ctgaattaat
     4561 tatattagtg cttatggtta tgatcattgc tatgttgatc ataccattac ctacatacct
     4621 tgttgatttt ttaatagggc ttaatattgt acttgctatt ctcgttttta tgggatcatt
     4681 ttatattgaa agaatattga gtttttctac attcccttcg gttttgttga taacgacatt
     4741 atttcgtctc gctttatcaa taagtactag tcgactaatt ttagttgatg cggatgcggg
     4801 taaaattatt actacatttg gccagtttgt cattggtgat agtctggctg taggttttgt
     4861 tattttttca atagtaactg ttgtgcagtt cattgttata acaaaaggat ctgaaagggt
     4921 tgcggaggta gctgctcgct tttctcttga cggtatgccg gggaaacaaa tgagcattga
     4981 tgcggattta aaggctggaa ttattgatgc cgcaggagct aaagaaagac gcagtattct
     5041 ggaacgtgag agtcaattgt atggttcatt tgatggagca atgaagttca ttaaaggtga
     5101 tgcaattgct ggcatcatta ttatttttgt caatttaata ggaggtattt ctgttggtat
     5161 gagtcaacat ggaatgtccc tatctggtgc attatctact tataccatac taactattgg
     5221 tgacggattg gtctctcaga ttcctgctct gttaatttct ataagtgcag gatttatcgt
     5281 tacaagagtt aatggtgata gcgataatat gggacgtaac ataatgtccc agatatttgg
     5341 gaatcctttt gttctcattg ttacatcagc tcttgccttg gcgataggga tgttgccagg
     5401 ttttccgttt tttgtttttt tcctgatagc agttactttg acggctttat tctattataa
     5461 aaaggtcgta gaaaaagaaa aaagtctgtc tgagtctgat tctagtggct atactggtac
     5521 atttgatatt gataatagcc atgactcatc tttggcaatg atagaaaatc tggatgctat
     5581 tagttcagaa accgttcctt taattttatt atttgccgaa aataagataa atgcaaatga
     5641 tatggaaggt cttattgaaa ggataagaag tcagttcttt attgattatg gtgtcaggct
     5701 tccaactatt ttatatagaa caagcaatga gcttaaggtc gatgatattg ttttgttaat
     5761 aaatgaggtg cgtgccgata gttttaatat atattttgac aaagtgtgta ttacagatga
     5821 aaatggagat atagatgctc taggtattcc tgttgtttca acttcatata atgaacgtgt
     5881 tatttcttgg gtagacgttt catatacaga aaatctaact aatattgatg ctaaaattaa
     5941 aagtgctcaa gatgaatttt atcaccagtt gtcacaagct ttattaaaca acataaatga
     6001 gatttttggt atacaagaaa caaaaaatat gttagatcag tttgaaaatc ggtatcctga
     6061 tctattaaag gaagtcttcc gacatgtgac tatacagaga atttctgagg tattacaaag
     6121 attgcttgga gaaaatattt ctgttcgcaa tttaaaactt attatggagt ctttggcgct
     6181 ttgggctccc agagaaaaag atgtcataac attagttgaa catgtccgtg catcactttc
     6241 taggtatatt tgtagtaaaa tagctgtttc tggtgagatt aaagttgtga tgctttccgg
     6301 atatattgag gatgcaataa gaaaagggat aaggcaaacc tctggtggct ctttcttgaa
     6361 tatggatata gaggtttcgg atgaggtaat ggaaacttta gcacatgctt tgagagaatt
     6421 gagaaatgca aaaaaaaatt tcgttctttt ggtatcagta gatatacgta ggtttgttaa
     6481 aagacttata gataacagat ttaagagtat actcgttata tcgtatgctg agattgatga
     6541 agcatatacc attaatgtat taaagactat ttagtgaggt ttaaatatga gtaacattaa
     6601 tttagttcaa ttagttagag atagtctttt cacgattggt tgtcctccat caattattac
     6661 agatttagac agccattcag cgattaccat ttctttggat agtatgcctg ctattaatat
     6721 agctcttgtc aatgagcagg ttatgttatg ggctaatttt gatgcaccaa gtgatgtaaa
     6781 acttcagtca tctgcttata acatattaaa tttaatgtta atgaacttta gttatagcat
     6841 taatgagctg gtagagcttc atcgttctga tgagtactta cagttaagag ttgttattaa
     6901 agatgactac gttcacgatg ggattgtttt tgcagagatt ctgcatgagt tttatcagag
     6961 gatggaaatc ttaaatgggg tcttataatc aatgagctat acaaaattgc tcactcaatt
     7021 atcttttcct aatagaatct cggggccaat cttggaaaca agtcttagcg atgtttcgat
     7081 tggtgagatt tgtaacattc aggctggaat tgaaagtaat gaaattgttg caagagctca
     7141 ggttgtagga tttcatgatg aaaaaacaat attaagcttg attggaaatt ctcgtggact
     7201 ttcacggcaa acgttgatta agcccactgc ccagtttctt catacgcaag tcggccgtgg
     7261 attattggga gcagtagtca atcctttagg ggaggttact gataagtttg ctgttacaga
     7321 taacagtgaa attctttatc gacctgtaga taatgctcct ccgctatata gtgaaagggc
     7381 tgcaattgag aagccttttt taacaggtat taaggttatt gattctttac tcacgtgtgg
     7441 tgaaggacag cgaatgggga tttttgcgtc agctggttgt ggcaaaactt ttctcatgaa
     7501 tatgctcatt gaacatagtg gtgctgatat atatgttatt gggttaattg gtgagcgagg
     7561 tcgagaggtt actgaaacgg ttgattattt gaaaaactct gagaaaaaaa gcaggtgtgt
     7621 tttagtatat gcaacttcgg attactcttc ggttgatcgt tgtaatgctg catatatagc
     7681 cactgctata gccgaatttt ttaggactga aggacataaa gtagcgcttt ttattgattc
     7741 attaacaagg tatgccagag cattacgtga tgtggcctta gccgctggag aatcacctgc
     7801 cagaagaggc tatccggttt cggtttttga tagcttaccc agacttcttg aaaggccagg
     7861 aaagttaaag gcaggtggct ctattactgc attttacact gttcttttgg aggatgatga
     7921 ttttgctgat ccattagctg aagaggtaag atccatttta gatggacata tatatttgag
     7981 cagaaatcta gcccaaaaag gacaatttcc tgcaattgat tccttaaaaa gtataagcag
     8041 ggtatttaca caggttgttg atgaaaaaca tcgtattatg gccgctgcat ttcgggagct
     8101 actttctgaa atagaagagc taaggacaat tattgacttt ggtgaataca aaccggggga
     8161 gaatgcctct caggataaaa tctacaacaa aatatctgtt gttgagagtt ttctgaagca
     8221 agattatcga ctcggtttta cttatgagca gacaatggag cttattggtg aaacaattag
     8281 ataaggtttt aaaaatcaaa gataaatatc aacggtctgt taaattaatt gaagcacaca
     8341 tattaacact aaaaaaaaaa ctctttgtat cgagatgtgg aggcattaga taaaaggatt
     8401 atatacttcc tgcaactgga gaatgactta gaacctgtag gcgctcagag tgttagtcag
     8461 cttttcaata caagacgtaa gatcgctata gtaaaaaagc atattatcca atatcagtct
     8521 gaacgaattc ttctaaaggg aagaattgaa gaaattcaaa aagatattga tgaggcaaat
     8581 gcttctaaaa gaaaactgct tcataaagag agtaaaatat gtaaacggat tggcttgatt
     8641 aaaagaaata actttgccaa acagttgata cttgatgagt tatcacaaga ggatatgaag
     8701 tatggcatta gataatataa acctaaattt tagtagtgac aaacagatag aaaaatgtga
     8761 gaaactatct tcaatagata atattgatag tctcgttttg aagaagaaga ggaaggttga
     8821 aattcctgag tactctttaa ttgcatctaa ctattttact attgataagc actttgaaca
     8881 taagcatgat aaaggagaaa tttatagtgg cattaaaaat gcgttcgaac ttagaaacga
     8941 acgagcgaca tattctgata ttccagaatc aatggccatt aaagaaaata ttttgatacc
     9001 agatcaagat atcaaagcaa gggaaaaaat aaatatcggc gatatgaggg ggatcttttc
     9061 atataataag agtggaaatg cagacaagaa cttcgaaaga agtcatactt cttctgtaaa
     9121 ccctgataat ctgctagaat ctgataatag aaatggtcaa attggtttaa aaaatcatag
     9181 cttgtctatt gataagaata ttgctgacat catttcttta ctaaatggaa gtgttgctaa
     9241 atcatttgag ctgcctgtaa tgaataaaaa tactgcagac ataaccccat ccatgtcatt
     9301 gcaagaaaaa tcaatagttg aaaatgataa aaatgttttt caaaaaaata gtgaaatgac
     9361 ttaccacttt aaacagtggg gggctggaca ttctgttagt atttcagtgg agtctggttc
     9421 ttttgttcta aaaccgtcag atcaatttgt aggaaataaa cttgacttaa ttttgaaa
//
LOCUS       DAZOYF010000152         9428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZOYF010000152 DAZOYF010000000
VERSION     DAZOYF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.153"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHUOF_003381"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHUOF_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHUOF_003382"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHUOF_003382"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR0612426.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHUOF_003383"
                     /pseudo
     CDS             2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHUOF_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHUOF_003384"
     CDS             complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHUOF_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR0612427.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHUOF_003385"
     CDS             complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHUOF_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR0612428.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHUOF_003386"
     CDS             5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHUOF_003386"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR0612429.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHUOF_003387"
     CDS             6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHUOF_003387"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR0612430.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHUOF_003388"
     CDS             8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHUOF_003388"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR0612431.1"
                     /translation="MKGWDERNGGNLTLRLDDADIALYHDNFHPQPRYIPLSQPMPLL
                     ANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNEAVPTSELPAHFL
                     SHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVG
                     ILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDEAFGLIDTAEKSAQVLV
                     KVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHUOF_003389"
     CDS             9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHUOF_003389"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR0612432.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2291 a         2470 c         2468 g         2199 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaataatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
     1981 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
     2041 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
     2101 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
     2161 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
     2221 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
     2281 tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg
     2341 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
     2401 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
     2461 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
     2521 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg
     2581 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
     2641 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
     2701 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
     2761 aagccgctgc cgcacttggc gtcgatccac tgtatgttgc tctgccaagc tatgttgtca
     2821 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
     2881 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
     2941 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc
     3001 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
     3061 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
     3121 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
     3181 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
     3241 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
     3301 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
     3361 cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
     3421 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
     3481 tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
     3541 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
     3601 ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct
     3661 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
     3721 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
     3781 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
     3841 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
     3901 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
     3961 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
     4021 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
     4081 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
     4141 tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
     4201 ctgtcgctga atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt
     4261 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
     4321 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
     4381 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
     4441 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
     4501 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
     4561 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
     4621 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
     4681 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
     4741 tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
     4801 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga
     4861 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac
     4921 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
     4981 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
     5041 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
     5101 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     5161 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
     5221 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
     5281 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
     5341 aatccatcgt tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga
     5401 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
     5461 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
     5521 gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc
     5581 acaacagcaa ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt
     5641 caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
     5701 tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg
     5761 ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga
     5821 gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg
     5881 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
     5941 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta
     6001 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
     6061 cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct
     6121 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga
     6181 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
     6241 caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg
     6301 ggaaacggcg caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag
     6361 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt
     6421 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc
     6481 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat
     6541 cggcatcgag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
     6601 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
     6661 tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
     6721 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
     6781 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
     6841 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
     6901 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
     6961 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
     7021 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
     7081 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
     7141 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
     7201 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
     7261 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
     7321 gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt
     7381 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
     7441 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg
     7501 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
     7561 cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc
     7621 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
     7681 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
     7741 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
     7801 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgcggcag
     7861 gcggtctggg aaatgtattg ccaacatcac gatacgccag caggtagcga atggctggag
     7921 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
     7981 gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc
     8041 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
     8101 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
     8161 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
     8221 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg
     8281 ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta
     8341 tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact
     8401 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga
     8461 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct
     8521 ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca
     8581 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
     8641 caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact
     8701 gtgggaaggc agcaccgaat gtctggtagt attcccggat ggcgttggca ttctgccgtg
     8761 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
     8821 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt
     8881 tggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
     8941 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
     9001 tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt
     9061 catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc
     9121 ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa
     9181 agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt
     9241 agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
     9301 cccagataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
     9361 caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
     9421 attacatc
//
LOCUS       DAZOYF010000153         9421 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZOYF010000153 DAZOYF010000000
VERSION     DAZOYF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.154"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="ACHUOF_003390"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="ACHUOF_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR0612433.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="ACHUOF_003391"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="ACHUOF_003391"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR0612434.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHUOF_003392"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHUOF_003392"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR0612435.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHUOF_003393"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHUOF_003393"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR0612436.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHUOF_003394"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHUOF_003394"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR0612437.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHUOF_003395"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHUOF_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR0612438.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6165..6800)
                     /gene="nth"
                     /locus_tag="ACHUOF_003396"
     CDS             complement(6165..6800)
                     /gene="nth"
                     /locus_tag="ACHUOF_003396"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR0612439.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="ACHUOF_003397"
     CDS             complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="ACHUOF_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR0612440.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="ACHUOF_003398"
     CDS             complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="ACHUOF_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR0612441.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="ACHUOF_003399"
     CDS             complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="ACHUOF_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR0612442.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9182..>9421)
                     /locus_tag="ACHUOF_003400"
     CDS             complement(9182..>9421)
                     /locus_tag="ACHUOF_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR0612443.1"
                     /translation="AKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQAN
                     AEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
BASE COUNT         2396 a         2363 c         2397 g         2265 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tctttttttt acatcccata
     5581 ttaacggggg tgctttctcg cgttatgtct aaaaaacata cgcgcaattg ttatagtggg
     5641 aggggaaacg gcaggtaatg ttcactatct tgcactttca gacaagtcct ttgtaataat
     5701 ctgttacata taactgaggc ggggaattca accattccgg gaaatgttat ccagcgttaa
     5761 atttttcaaa aaacgtaacg gcagattttt tcactgcatt gagaggggga tgatggcttg
     5821 gcggcgatat gttctgctat ttagcgtttt tcctagtgag atagtccagt ttctgaaaaa
     5881 tagccagtgt aatgttttgc aggtcaatat ttgttgcgtg tgtaaattta aatcatttta
     5941 acctgattct tgcaatgttt ttacattgcc acagcatcgt gatcttgatc acgaatgaat
     6001 agaaaatttc gctattaaaa aaacaattaa cctgcatttt tggttatccg caaacggatt
     6061 acctgatgat aacgctaagc aatggcatca ttgacgttaa aaagatgttt taaaagagga
     6121 taaagaaagg ttatcaatgg ggtaatcggt gttacccctt ttcttcagat gtcaactttc
     6181 tctttgtatt cacaaagatc ttcaataata caagagccac agcggggctt gcgggcaatg
     6241 caggtataac gcccgtgcag gatcaaccaa tggtggcagt cgactttaaa ctctgccgga
     6301 accactttca gtagcttttc ttctacctgt tcgacgtttt tccccggcgc aaattgagta
     6361 cgattacaaa cgcggaaaat gtgcgtgtcg acagcaatag tcggccagcc gaatgcagtg
     6421 tttaatacga cgttggctgt tttacgacct acgccgggca gggcttcaag cgcagcacga
     6481 tcttccggaa cctcgccatt atgttgttcc agtaatatac ggcaggtttt gatgatattt
     6541 tctgctttgc tgttataaag gccaatcgtt ttgatatagg ttttcacccc ttcaacgcct
     6601 agttcaagca tcgctgcagg cgtattcgcc accgggtaga gtttcgccgt cgccttatta
     6661 acactgatat cggtcgcctg agcggaaagc agtacggcaa tcagcaattc aaaaggcgaa
     6721 ctgaaattaa gctcggtggt gggatgagga ttgttctcac gcaggcgagt gaggatctcc
     6781 agacgttttg ctttgttcat cagacattcc ctgtttcacc gtttggcaat gcacgttcgg
     6841 cagctgcttc agcacggcgc tttttcattc tttcatcaat caggtatttt cctgccagca
     6901 tcagtcccaa gccaataaat gcacctggtg gcagcatcgc cagcaggaaa ggggagtcgg
     6961 tgtggaaaat ctccacgcgt aatacttttg cccagctacc taacagcgca tctgcaccgt
     7021 caaacaacgt gccattgccg ataatttcgc gtagtgaacc cagcacgaac atggcgcagg
     7081 ttgcgcccgt accaattgaa aagccgtcca gtgccgaaag cgccggacct tttttggcgg
     7141 cgaaggcttc agcgcggccc acaacgatac agttagtgac aatcagcgga ataaaaatcc
     7201 ctaatgattg atacaggcca aaggcgtagg cgttgatcag catctgtaca gcgctgacca
     7261 ccgaggcgat gatcatcacg taaatgggaa tgcggatctc ggctggcgtc cagtgacgca
     7321 gcgtcgaaat ggtcaggttg gtcagcgtca gtaccagcgt agtcgcaagt cctaaaccca
     7381 gagcgttagt ggcagtggat gtgaccgcca atagcggaca aaggccgagc agttggacca
     7441 gtgcagagtt gtttttccac aacccctgaa caataacgtc tttaatttcg ctcacgcttt
     7501 attctccaca ggcaggaagt tgagaaagtt gtgccggtaa cgtctgagcg tacaatccgg
     7561 cgcgttttac cgcattaacc accgcgcggg gagtaatcgt cgcgccggtg aactggtcga
     7621 aatcaccacc atctttcttc accgcccagt gcgcatcatc cgcaccactg attttttttc
     7681 ccgcaaaatg ggtgagccag tcagaaaggc gcaattcgat tttatcgcca agccctggcg
     7741 tttcgtggtg ctctgtcacg cgcgtgccaa gtaccgtgcc attaaaatcg gctcccacca
     7801 gcagctgaat cgcaccggaa tagccatctg gcgcggttgc ttccagaacg gcggctaccg
     7861 gtttgtcatc ctgtttggcg atgtaaaccc gatgctcacc tttacctaac tctggcgcag
     7921 ttaccagata gcaactctgt gcgagcgcat tgttatagcg ttcggctggc agcacctgat
     7981 caaataacgt cttttgttgc agactggcct gttcagcaat tgtcgttttg gtcatctggt
     8041 tgatggccgc agttaaccct gtagaacccg ctgcaaatag tgccagtgta atgccgtgtt
     8101 ttcggatagt tttcagcatg gtttaccctt tgcgatggcc gtagacgcgc ggacgcgtgt
     8161 agtaatcgat cagaggaacc gtgatgttcg ccagcaggac ggcaaaagcc acgccgtcag
     8221 gatagccgcc gaaactgcgg atcaaccaga ctaataagcc cgccagcgcg ccgaacatca
     8281 gccgaccacg attggtcgta gaagcagtaa ctgggtcagt caaaataaag aatgcgccga
     8341 gcatagtcgc accagacagc agatgaattt gcggtgctgc cagtgtttct ggtgagaaca
     8401 accagcccaa cgttgcgcat aacgccagcg ttactaagaa gctgagggga atatgccagc
     8461 gaatcgcttt ctgccatagc aaccacacgc caccagccag ccaggcgaga tttacccatt
     8521 gccaaccagc gcccgccaga ataccgctgt agatcggata ttgcataatc tgttcaaccg
     8581 aatgacctgc gcggacagag gttttgaatg tatccagcgg tgtcgcctga ctaatgccat
     8641 caatacctaa gcgtagcgta ttcatatcac caccactggc ggtatgaccg ctgaaaataa
     8701 cctgtatggc gtcgataaaa ccagggatgt tgaccgcaat ttcatgtggc ggtaaccagc
     8761 tggtcatctg caccgggaag gagatcagta agaccacata accaatcatt gccggattaa
     8821 acgggttttg ccccagaccg ccatacaact gtttagcgat gatcaccgca aacaccgtac
     8881 ccagtacgac catccaccat ggcgctaggg ggggaatact taccgccagc aataagcctg
     8941 tcagcaatgc tgagttatct ttcaacgttg cggctaccga ctgcttgcgt agtttgagta
     9001 cgagagcttc ggctaacaga gcactgaccg acgccaacag gatctgaacg agagtacccc
     9061 aaccaaaaaa ccgcagttgc gctgcgattc ctggcacggc tgcgagcaac accaacagca
     9121 taatgcgcga tgtctggcgc tggttatggg tataagggga gctagctatt ctgaatacca
     9181 tttagtcctc gtttacaacc ttttgctggg cggctttttt ggcctgaacg cgggcaatag
     9241 ccgcggcaac tgcggctttg cgcggatcga cctgttcttc tggttccgca ttagcctgtt
     9301 gctgttccag cttgcgtgct ttggcgcggg caatagcggc ttcgacggca gctttgcgcg
     9361 gatcaacctg ttcttctggc tccgcattgg cctgttgctg ttccagcttg cgtgctttgg
     9421 c
//
LOCUS       DAZOYF010000154         9412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZOYF010000154 DAZOYF010000000
VERSION     DAZOYF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.155"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            70..222
                     /gene="hokE"
                     /locus_tag="ACHUOF_003401"
     CDS             70..222
                     /gene="hokE"
                     /locus_tag="ACHUOF_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0612444.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            573..725
                     /gene="hokE"
                     /locus_tag="ACHUOF_003402"
     CDS             573..725
                     /gene="hokE"
                     /locus_tag="ACHUOF_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0612445.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(847..1467)
                     /gene="entD"
                     /locus_tag="ACHUOF_003403"
     CDS             complement(847..1467)
                     /gene="entD"
                     /locus_tag="ACHUOF_003403"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR0612446.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="ACHUOF_003404"
     CDS             complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="ACHUOF_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR0612447.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4125..5327
                     /gene="fes"
                     /locus_tag="ACHUOF_003405"
     CDS             4125..5327
                     /gene="fes"
                     /locus_tag="ACHUOF_003405"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR0612448.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5330..5548
                     /gene="ybdZ"
                     /locus_tag="ACHUOF_003406"
     CDS             5330..5548
                     /gene="ybdZ"
                     /locus_tag="ACHUOF_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR0612449.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5545..9390
                     /gene="entF"
                     /locus_tag="ACHUOF_003407"
     CDS             5545..9390
                     /gene="entF"
                     /locus_tag="ACHUOF_003407"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR0612450.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2049 a         2477 c         2598 g         2288 t
ORIGIN      
        1 cctggggctg ccactccaaa ccagacaatt tggatggtag tcccttcttc gcatggaggt
       61 aatataaaca tgctgacgaa atatgccctt gtggcagtca tagtgctgtg tttgacggta
      121 ctgggattta cgcttctggc cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat
      181 attgagttca gggctgttct cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa
      241 tccccgcata tccggttgtc aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg
      301 cggtcagtga tggctgcggg cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga
      361 agtccgaaac aggactcact gtataaataa acagctattt tgctaaggaa gggtaagata
      421 acggcgggtg cctgaagctt tccggtttca ggttaactct gaggtttgga aagatgaagc
      481 cccaagagat atttctatca atcctggggc tgccactccg aacctcaaca actcggatgg
      541 tagtcccttc ttcgcatgga ggcaatataa acatgctgac gaaatatgcc cttgtggcag
      601 tcatagtgct gtgtttgacg gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg
      661 agttcaccgt aaaggaacgt aatattgagt ttaaggctgt tcttgcttac gaaccgaaga
      721 agtagccgtt gtgcggggag taatccccgc atatccggtt gtcaggtcag agtggtaagg
      781 cacctgcttt acactttcgc ccgcagtcag tgatggctgc gggcgaatcg taccagatgt
      841 tgtcaattaa tcgtgttggc acagagttat gactattttt tcttttatct gccagtgcac
      901 agcaaacatc tcattctcac gatgaatgat gacctgctgt ttattccagc taattatctg
      961 atagtccaga aaacctgcat cagtttggat ctcacttgcc ttaaatgcgc tctctttggc
     1021 ggaaaatgca agtgtcagcg ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg
     1081 ttctgctggt gtaataatgt tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc
     1141 ttcgatatca acgccaatcg gttgacggga aaccacggcc aatgccgtag ccccgcagtg
     1201 gctaatactg ccgtatacct ccgcaggcca caccggttgg cgtagctcgc cgattgcggg
     1261 cacacattta tagccatatt cccgcaaggc ataaacagca gcgatccgtc cggctaaatg
     1321 ctctgtttta cgtttacgtc cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa
     1381 atcctgctca caaaaattcg ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa
     1441 ggggagggag gtatgcgtag ttttcatatc gaccatattc gagactgatg acaaacgcaa
     1501 aactgcctga tgcgctacgc ttatcaggcc tacgtgatcc tgcaatatat tgaattggca
     1561 agatctttgt aggccggata aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt
     1621 gtcaacaatc tgacgttagc atcagaagtg agtattaatg ctcatatacc acgtacgtcc
     1681 cggctcgtta taggtatacg cgccggcacc ggcgatatag ttggctcctg ccagatcgcc
     1741 cgtggtctgg gcattacctg cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc
     1801 ggtcagactg acattcttcg tcacatccca ggtcgcgctc agacccacaa tgctgtaagg
     1861 actgatttct ttggtttccg ggccaaccgc tggctgacct ttatagttgt acttcttcgg
     1921 ctgctgcttg ccgtaccagg tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca
     1981 gctcagcgtt gagttcaacg tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt
     2041 gttttcactc ttcagcatat aagtgatgtt attggtccac atcaccgttt cgctaactgg
     2101 tacgtttaac gatccttcca gaccttcaac cactgctttc ggcacgttat cccactgata
     2161 gagatcagtg ccgactgcgt tttgccctac agccacatag cccgcttcaa tcttattgcg
     2221 ataatcgtta cggaaccagg tgacgcccgc cagccagccg tcgcgtttga actccagacc
     2281 aatctctttg ttgatgctgg tttctacttt caggtcatcg ttaccttgca gatagcagcc
     2341 gcccgcgctg gcatagcagc cctgaccttt actgtagaga atgtagttcg ggttagtctg
     2401 gtataggctc ggcgctttat aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc
     2461 taaaccttgc gatatgttca gcgccgggct ccagttattg ccgacaatac tgtgatgatc
     2521 gaaacgcagc cccggcgtta cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa
     2581 cagcgagaaa atttctgctt ttgaatacgg gctacggtcg gtggcactca cgccatcaat
     2641 agcgccaccg gtattcgttc cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg
     2701 ttgctgattc cactccgtac ccagcgtcag cgtctggtta acgaggaaat caatcggcag
     2761 gttaacttcg ctgtgcagca tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc
     2821 tttttcgtta aatttccctt cggtaccgcc cgccagacct tccggaatac gcgagttacg
     2881 ggtgtgttca tactgcaccc agttgctggt ggtcacgccg ttatcccagc caccgttcca
     2941 ggtcagcgcg tagttctggc gatacaggcg gttggtttca tcgccatatt tcgagcgagt
     3001 gtaagcgtca gagttggtgt tctgggtatc gcccgcatac aggttaccct ggcggctgta
     3061 acccgcttcc agttccagcg attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat
     3121 atctttattg atcacccctt cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg
     3181 cgcggactga tggccctggt tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc
     3241 atacaagcgg aagctgaatt cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt
     3301 ggtggcacct tcctctttat gttctggcgc attgaaatat gcgtcccagg agccgtgcca
     3361 ctcgccgctg ccttttttgg taatgatgtt aaccacgccg cccgccgcgc cgttgccata
     3421 acgcgcagct gccggaccac gcagaacttc aatacgttca atcatttcag gtggcaccca
     3481 ggaggtatca ccacgggtat cgcgctcgcc acgccagccc tgacgcaccg agttacggct
     3541 gcttaccggc ttgccgtcaa tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc
     3601 aatctgtcgg ttattcccac gctgaccact ggtggagtta ccggtcaggt taacgcctgg
     3661 catggtacgg atgatctccg acacatcgcg ggcaaccggg tttttgcgga tttcatctgc
     3721 ggtgatggtc gaaacgccag gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt
     3781 atcgtcatgt gaaacaggtg tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc
     3841 cagattgacc aacaaggcca gggaatgaat cttcttgttc attgttttat tcctgcattt
     3901 ttgccacgaa ttgcaactgt cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc
     3961 gaaaaccttt gatcctgaaa gacacgcagt gcagttggtt aattaatgtc cgcgcttccc
     4021 acggcgcgcc attacgctat tgcaaatgca aatagttatt aataacatta tcaatatatt
     4081 tctgcaatca atgaaaaatt gcacagtaaa catggggtta tggtgtgacg gcgttaaaag
     4141 taggaagtga gagctggtgg cagtcgaaac atggcccgga atggcagcgt ctgaatgacg
     4201 aaatgtttga ggtcactttc tggtggcgtg atccccaagg ttctgaagaa tactcgacga
     4261 taaagcgcgt atgggtctac atcactggtg tgaccgatca tcatcagaac agccagcccc
     4321 agtcgatgca gcgaattgca ggtactgatg tctggcagtg gacgacacaa ctcaatgcca
     4381 actggcgcgg cagctactgc tttattccca ccgaacgcga tgacattttt tctgcaccat
     4441 cccccgatcg cctcgaatta cgtgaaggct ggcgaaaact attaccccag gcaatagccg
     4501 atccgctgaa tccacaaagc tggaaaggcg ggctagggca cgctgtttct gcactcgaaa
     4561 tgccgcaagc gcctctgcaa ccgggatggg attgtccgca agcgccagaa ataccagcca
     4621 aagaaattat ctggaaaagt gaacggttga aaaattcacg tcgtgtatgg atttttacca
     4681 ccggcgatgt aacagcagaa gaacgcccgc tggcagtttt gctcgatggc gaattttggg
     4741 cgcaaagtat gcccgtctgg cctgtgctga cttcgctgac ccatcgtcag caacttcctc
     4801 ccgccgtgta tgtgttgatc gacgctatcg acaccacgca ccgcgcccac gaactgccgt
     4861 gtaatgcgga tttctggctg gcagtacagc aagagttatt acccctggtg aaagttattg
     4921 ccccttttag cgatcgcgcc gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt
     4981 ccgcgctgta tgccggactg cactggcctg aacgctttgg ctgtgtatta agccagtcag
     5041 gatcgtactg gtggccgcat cggggcgggc agcaagaggg cgtgttactt gaaaagctaa
     5101 aagctggtga agttagcgcc gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc
     5161 cgatgatcat gcgggccaat caggcgctgt atgcgcaatt acaccctata aaagaatcca
     5221 ttttctggcg tcaggttgac ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc
     5281 aggggttaat cgacctctgg caaccacttt tccatgacag gagttgaata tggcattcag
     5341 taatcccttc gatgatccac agggagcgtt ttacattttg cgcaatgcgc aggggcaatt
     5401 cagtctgtgg ccgcaacaat gcgccttacc ggcaggctgg gacattgtgt gtcagccgca
     5461 gtcacaagag tcctgccagc agtggcttga agcccactgg cgtactctga caccggcgaa
     5521 tttcacccag ttgcaggagg cacaatgagc cagcatttac ctttggtcgc cgcacagccc
     5581 ggcatctgga tggcagaaaa actgtcggat ttaccctccg cctggagcgt ggcgcattac
     5641 gttgagttaa ccggagaggt tgatgcgcca ttactggccc gcgcggtggt tgccggatta
     5701 gcgcaagcag atactttgcg gatgcgtttt acggaagata acggcgaagt ctggcaatgg
     5761 gtcgatgatg cgctgatatt cgaactgcca gaaattatcg acctgcgaac caatattgat
     5821 ccgcacggta ctgcgcaggc attaatgctg gcggatttgc aacaagattt gcgcgtcgat
     5881 agcggtaaac cactggtctt tcatcaactg atacaggtgg cagataaccg ctggtactgg
     5941 tatcagcgtt atcaccattt actggtcgat ggcttcagtt ttccggccat tacccgccag
     6001 atcgccaaca tttactgcgc attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg
     6061 cctttcgctg atgtagtgga agagtaccag caataccgcg aaagcgaagc ctggcagcgt
     6121 gatgcggcat tctgggtgga acagcgtcgc caactgccac cgcccgcgtc actttctccg
     6181 gcacctttag cggggcgcag cgcctcggca gatattttgc gtctgaaaat ggaatttacc
     6241 gacggggaat tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc
     6301 cttgcgctga cggctttgtg gctggggcga ttgtgcaatc gcatggacta cgccgccgga
     6361 tttatcttta tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac
     6421 gttttgccgc tgggtattca tattgcggcg caagaaacgc tgccggaact ggcaacccga
     6481 ctggcaaccc gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc
     6541 gaacaaattg tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta
     6601 ctcaatatta aggtatttga ttaccaactg gatattcctg gtgttcaggc gcaaacccat
     6661 accctggcaa ccggtccggt taatgacctt gaactggccc tgttcccgga tgaacacggt
     6721 gatttgagta ttgagatcct cgccaataaa cagcgttacg atgagccaac gttaatccag
     6781 catgctgaac gcctgaaaat gctgattgcc cagttcgccg cggatccggc gctgttgtgc
     6841 ggcgatgtcg atattatgct gccaggtgag tatgcgcagc tggcgcagat caacgccact
     6901 caggttgaga ttccagaaac cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca
     6961 ccggatgctc cggcgctggc agatgcgcgt tacctgttca gctatcggga aatgcacgag
     7021 caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg
     7081 gcggtggcac taccgcgctc ggtctttttg accctggcac tccatgcaat agttgaagct
     7141 ggcgcggcct ggctaccgct ggataccggc tatccggacg atcgcctgaa aatgatgctg
     7201 gaagatgcgc gtccgtcgct gttaattacc accgacgatc aactgccgcg ctttagcgat
     7261 gttcccaatt taaccaacct ttgctataac gccccgctta ccccccaggg cagtgcgccg
     7321 ctgcaacttt cacaaccgca ccacacggct tatatcatct ttacctctgg ctccaccggc
     7381 aggccgaaag gggtaatggt cgggcagacg gctatcgtca accgcctgtt gtggattcaa
     7441 aatcattatc cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat
     7501 gtctcggtgt gggagttttt ctggccgttt attgccgggg ctaaactggt gatggctgaa
     7561 ccggaagcgc accgcgaccc gctcgctatg cagcaattct ttgccgaata tggcgtaacg
     7621 accacgcact ttgtgccgtc gatgctggca gcatttgttg cctcgctgac tccgcaaacc
     7681 gctcgccaga gttgcgtgac gttgaaacag gttttctgta gtggtgaggc cttaccggct
     7741 gatttatgcc gcgaatggca acagttaacc ggcgcgccgt tgcataacct gtatggcccg
     7801 acggaagcgg cggtagatgt cagctggtat ccggcttttg gcgaggaact ggcacaggtg
     7861 cgcggcagca gtgtgccgat tggttatccg gtatggaata cgggtctgcg tattcttgat
     7921 gcgatgatgc atccggtgcc gccgggtgtg gcgggtgatc tctatctcac cggtattcaa
     7981 ctggcgcagg ggtatcttgg acgacccgat ctgaccgcca gccgctttat tgccgatcct
     8041 tttgctcctg gtgaacggat gtatcgtacc ggagacgttg cccgctggct ggataacggc
     8101 gcggtggagt acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa
     8161 ctgggcgaaa tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc cgttacccac
     8221 gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat
     8281 ctggtatcgc aatcgggcct gccgttggat accagcgcat tgcaggcgca gcttcgcgaa
     8341 acattgccgc cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc
     8401 gccaacggca agctggatcg caaagcctta ccgttgcctg aactgaaggc acaagcgcca
     8461 gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg cggcattcgc gtcgttgctg
     8521 ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca ttcgctactg
     8581 gcaatgaaac tggcagcgca gttaagtcga cagtttgctc gtcaggtgac gccggggcag
     8641 gtgatggtcg cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc
     8701 tcccggcgca tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg
     8761 ttttgtttcc atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc
     8821 gatccacaat ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca
     8881 gcgacaaacc tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg
     8941 cacggcattg cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg
     9001 gatacctggc cgccagagac gcaaaactgg caggaaaaag aggctaatgg tctggacccg
     9061 gaagtgctgg cggagattaa ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt
     9121 acttcaacgg agttgtttac caccattgaa ggcaactacg ctgatgctgt gcgcctgctg
     9181 acgactgctc atagcgtacc gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg
     9241 cttcaggaag gtatgagccc cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc
     9301 tatcgtcagg attgtgcgca tgtggatatt atctctccag gggcgtttgt aaaaattggg
     9361 ccgattattc gcgcaacgct aaacaggtaa attaatatta tctataaacc ca
//
LOCUS       DAZOYF010000155         9391 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZOYF010000155 DAZOYF010000000
VERSION     DAZOYF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.156"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="ACHUOF_003408"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="ACHUOF_003408"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0612451.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="ACHUOF_003409"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="ACHUOF_003409"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFR0612452.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="ACHUOF_003410"
     CDS             3115..3399
                     /locus_tag="ACHUOF_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0612453.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACHUOF_003411"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACHUOF_003411"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR0612454.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACHUOF_003412"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACHUOF_003412"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR0612455.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACHUOF_003413"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACHUOF_003413"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0612456.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQSLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQSHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="ACHUOF_003414"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="ACHUOF_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR0612457.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2243 a         2480 c         2389 g         2279 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggctctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcga ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa t
//
LOCUS       DAZOYF010000156         9312 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZOYF010000156 DAZOYF010000000
VERSION     DAZOYF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.157"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..1248
                     /locus_tag="ACHUOF_003415"
                     /pseudo
     CDS             1..1248
                     /locus_tag="ACHUOF_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            1471..1968
                     /gene="ubiC"
                     /locus_tag="ACHUOF_003416"
     CDS             1471..1968
                     /gene="ubiC"
                     /locus_tag="ACHUOF_003416"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR0612458.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1981..2853
                     /gene="ubiA"
                     /locus_tag="ACHUOF_003417"
     CDS             1981..2853
                     /gene="ubiA"
                     /locus_tag="ACHUOF_003417"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR0612459.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(3008..5431)
                     /gene="plsB"
                     /locus_tag="ACHUOF_003418"
     CDS             complement(3008..5431)
                     /gene="plsB"
                     /locus_tag="ACHUOF_003418"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR0612460.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            5602..5970
                     /gene="dgkA"
                     /locus_tag="ACHUOF_003419"
     CDS             5602..5970
                     /gene="dgkA"
                     /locus_tag="ACHUOF_003419"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR0612461.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            6080..6688
                     /gene="lexA"
                     /locus_tag="ACHUOF_003420"
     CDS             6080..6688
                     /gene="lexA"
                     /locus_tag="ACHUOF_003420"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR0612462.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6761..8086
                     /gene="dinF"
                     /locus_tag="ACHUOF_003421"
     CDS             6761..8086
                     /gene="dinF"
                     /locus_tag="ACHUOF_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR0612463.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            8202..8411
                     /locus_tag="ACHUOF_003422"
     CDS             8202..8411
                     /locus_tag="ACHUOF_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR0612464.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(8453..8968)
                     /gene="zur"
                     /locus_tag="ACHUOF_003423"
     CDS             complement(8453..8968)
                     /gene="zur"
                     /locus_tag="ACHUOF_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR0612465.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         2244 a         2154 c         2432 g         2482 t
ORIGIN      
        1 acaaattgtc attttaaagg tgacgttatt tctaaggtgt cttttttatc atcaaatcta
       61 caacatgtaa ctttcgaatg taaagaaatt gagaattgca attttactaa ggcaacagtt
      121 gataatgtca tatttaaatg tcgacgttta cacaatgtga tttttctcaa aacgagtggc
      181 gaatgtgttg attttagcca aaatattctt gatacagttg acttctcgca gagtcaactt
      241 ggtcatagta attttcgcga atgtcagatt agaaattcaa acttcgataa ttgttatctt
      301 tacgcttcgc acttcaccag agcagagttt ctgtctgcca aagaaatatc atttattaaa
      361 tcgaatttga cagctgttat gtttgatcat gtgcgaatgt cgacagggaa tttttaagat
      421 tgcattacag aacaattgga attaactatt gattattcag atatatttgg gaatgaagat
      481 ctcgatggtt atatcaataa cattataaaa atgattgata cattgccaga taatgcaatg
      541 atattgaaat ccgttctggc agtaaaactg gtgatgcaat taaaaattct caatattgtt
      601 aataaaaact ttattgagaa tatgaagaaa acatttagcc attgtcctta tataaaagat
      661 ccaattatac gtagttatat acattctggg gaagatgaca agttcgataa ttttatgcgt
      721 caacatcgat tcagtaaggt gaatttcgat acccaacaga tgatccattt tattaacaga
      781 tttaatatga ataaagggct ggttgataaa aataacaatt tttttatcca acttatcgat
      841 caggccttac gatcaacgga tgatatgatc aaagcaaatg cctggtatct ttataaagag
      901 tggattcgta gtgatgatgt ttcacctata tttatagaaa ctgaagaaaa gttaagaacc
      961 tttaacacta ataaattaac acgaaacgat aatatattta tcctgttctc ctctgtcgat
     1021 gatgggcctg ttatggtggt aagctcccag cgcttacatg atatgttgaa tcctacaaaa
     1081 gataccaatt ggaattccac gtgtatctat aaatccagac ataagatgtt gcctattaat
     1141 cttactcagg aaacactttt cagctccaaa tctcatggta aatatgcgct tttcccaatt
     1201 tttactgcga gttggcgagc tactcgtata aagaataaag gtatttaagt aaaggaaaac
     1261 atcaccgttc ctagcatcat gggcggtgat gcccctatgg ttgccctcgc cagcacgggc
     1321 atcggcaaag cgtaaggttc aacagcgttt taccacttca tgcgattgtt gcgtttttgt
     1381 tgcgcattag atcacttaat ttgctttaca tctcccgtaa acacttttct gcgatacaat
     1441 gcctttacgt tatgtaacgg agagttcggc atgtcacacc ccgcgttaac gcaactgcgt
     1501 gcgctgcgct attttaaaga gatccctgcc ctggatccgc aactgctcga ctggctgttg
     1561 ctggaggatt ccatgacaaa acgttttgaa cagcagggaa aaacggtgag tgtgacgatg
     1621 atccgcgagg ggtttgtcga gcagaatgaa atccccgaag aactgccgct gctgccgaaa
     1681 gagtctcgtt actggttacg tgaaattttg ttatgtgccg atggtgaacc gtggcttgcc
     1741 ggtcgtaccg tcgttcctgt atcaacatta agcgggccgg aactagcgtt acaaaaattg
     1801 ggtaaaacgc cgttaggacg ctatctgttc acatcatcga cactaacccg ggattttatc
     1861 gagataggcc gtgatgccgg actgtggggg cgacgttccc gcctgcgatt aagcggtaaa
     1921 ccgctgttgc taacagaact gtttttaccg gcgtcaccgt tgtactaaga ggaaaaaaat
     1981 atggagtgga gtctgacgca gaataagctg ctggcgtttc atcgcttaat gcgtacggat
     2041 aagccaattg gcgcgttact gctgctctgg ccaacattat gggcgttgtg ggtggcgaca
     2101 ccgggcgttc ctcagctctg gatcatggcg gtgtttgtcg cgggtgtctg gctgatgcgc
     2161 gctgctggtt gcgtggtgaa tgattatgct gatcgcaagt ttgatggtca tgttaagcgc
     2221 acggcgaacc gaccacttcc cagcggcgcg gtaacagaga aagaggcgcg cgcgctgttt
     2281 gtagtgctgg tactgatttc gtttttactg gttctgacgc tgaatacgat gaccattctg
     2341 ttgtcgattg ccgctctggc attggcgtgg gtgtacccgt ttatgaagcg gtatacccat
     2401 ctcccgcaag tggtgctggg cgcggcgttt ggctggtcga ttccaatggc ttttgccgct
     2461 gtgagtgagt cggtgccatt gagttgctgg ttaatgttcc tcgccaatat tctctgggcg
     2521 gtggcttacg acacgcagta tgcgatggtt gaccgcgatg atgatgtgaa gattggcatt
     2581 aaatccacgg caatcctgtt cggccaatac gataaattga ttattggtat tttgcagatt
     2641 ggcgtactgg cactgatggc gatcatcggt gagttaaatg gcttaggctg gggatattac
     2701 tggtcaattg tggtggctgg cgcgctgttt gtttatcaac aaaaactgat tgccaaccgc
     2761 gagcgtgaag cctgctttaa agcatttatg aataataact atgttggtct ggtactgttt
     2821 ttaggactgg caatgagtta ctggcatttc tgatgatgta aaaaagccgg atgatcatcc
     2881 ggctttcttc tgggttgcct gatgcgttgc gcttctcagg cctacccggt gaatcgcaat
     2941 ttattgaatt tgcaggtcat ggtaggccgg ataaggcgtt ttcgccgcat ccggcaattc
     3001 tctctgatta cccttcgccc tgcgtcgcac tctcaatcgt caaacgcacg tctgatgtaa
     3061 tcaactccgc cagcaactga taaaccttca tcgtttctgc tggttcggca tcgccgctat
     3121 cgctgatata cccttcatca cgcagcgtca gcaccagaga actgaacacc gccttgtcga
     3181 agaactccgg cgcgttgatg ccgtgcagca cggagagacg ttgcgcaacg gtgcggctct
     3241 ctttctccag cgtaccgcgg ttgatcgacg ggttggcact caacaaccag aaggtgatgg
     3301 cataacgttg cagcgtttcg cgcgcgcctg cggccagcag ctgtagggtg cgagaatgcg
     3361 ccgggttgat atgcaactca tcatcttgca gggtaatcag cccctgacgt tgcatctcat
     3421 ttgccagcgc atcaataacg tccggcaact cgtcgcgatc ccagcgcagg aacagctccg
     3481 ctttcagcat tgggtaaagc acattgacgt gctccatcaa tacgtcgcgg gagatgtggc
     3541 gatgctgggt gacaattgcc gccatcagcg aaggcagcac caacatatgc gcaatgttgt
     3601 tgcgatagta ggtcatcagc accgcttgct cgcgtggcag aatgatgatg tcgccgatag
     3661 tgtctttctc gacttcaaac ttgttcattt gcagcgcgtg atcgataagc tcgctggcgc
     3721 tggctgaagg aacggtagag tccgtggagt aaggtacgtt gcgcatcaga tccaggtagc
     3781 agttgagttg ctcggttaac tgctcgcggg tgagtgagcg ctgacgtgat gccagtagcg
     3841 cagtacagca caggttcatg gcgtttgccg cgcctgcgtt gttaatgcgt accatcagat
     3901 cggcagcaat attattgacc gtcggcgtta accaggccgg acgcaccgct tcgatgggat
     3961 cgatagattc acgccagtct ggtacgtgct ggttaaggta ggtcatcaac ggcattggtt
     4021 caccgaagtt gacgtaaccc tgaccgagat tacgcagctt gcttaaaccg cgcagcatct
     4081 gcggcaggct ctctttctct ttcgtcgcgc cgcgcagttc tttggcgtaa gtacccactt
     4141 ccatgacgtg ctcataaccg atatagatcg gaatcagcgt aatcggacgc gtgccgccac
     4201 gcagcatcgc ctgaatggtc atcgacagcg taccggtttt cggatccagc aaacgccccg
     4261 tgcgggaacg accgccttcc acgaagtact cgacggaata accacggctg aacagttcgc
     4321 cgagatactc acggaaaacg gtggaataaa gtttattgcc tttaaaagta cggcgaataa
     4381 agaacgcccc cagacggcgg aaaatcggcc cggccggcca gaagttcagg ttgatcccgg
     4441 cggcgatatg cggcggcacc agtccctggt ggtacagcac gtaagaaagt agcaggtagt
     4501 ccatgtgact gcggtggcaa ggcacataca ccagctcatg accgtcgtgg gccagctggc
     4561 gaacgcgctc ggcgttatgg acgttgatgc cctgataaaa tcggttccag gtgaagccca
     4621 gaatgcggtc agtcaggcgg atcatctcgt aagagaaatt cgccgcaatc tcttccatca
     4681 gcgcaatcgc gttctgctgc gctttttcat gggagatttt tttgctgcgc gcttcatctt
     4741 ctaccgcttt ggcaatggcg cgggaggcga gcagcttatt aaacagatcc tgacgagcag
     4801 gcagacgtgg gcctacggca gccagacgtt gacgggcaaa gtgcatacgc gccacgcgcg
     4861 ccagtttctg agcgatagtt ttatccgtgc cgtgttcatc cgccatacgg cgcagcgaca
     4921 ctgacggcga gaaacgcaca aaactgtcgc gaccgagcca cagtacagcg aaaaatttct
     4981 gtacgccgtt aagcatacgc agcggcgggt tcacttcgcc tttttcacgc cccggcgcgc
     5041 gaccaaacat caccgatact ggcaccatct gcacatccag atttgggttg ctacggtgca
     5101 aatcgagata gtcgtggaaa agcttaatag actcttcttt cggcgtgtaa taggtgaata
     5161 cacgcggccc gccgtgaatg aacacatagc gcggcagtag cgtgccgtcg atttccagcg
     5221 gctctaacgg gtcaggcaaa tcatgtgcca gacactgggc gcgcaacgtc agcaaatctg
     5281 ctttcgagtt gtacggtaaa acgtacataa ttggacgaga ggtatccagc cccagttccg
     5341 gggcaggatc tgccggaata gacttgcttt ttaccaggat gcttaatggt aaattcagta
     5401 atttgtagta aattcgtggc cagccggaca taaacgatgt aaagcctctg gttaataatg
     5461 caaatgcgcg gcaaggatag cagaaagtca tgggaaattc tgtggtatcc gctcatgttt
     5521 cgcgcggcgt tacgcaaacc cgaatcatcg gatttaacgg tacactgata ttgacgctca
     5581 taatgtaaaa aggttctttc aatggccaat aatacaactg gattcacccg aattatcaaa
     5641 gctgctggct attcctggaa aggtttacgc gctgcatgga tcaacgaagc ggcattccgt
     5701 caggaaggcg tagcggtatt gttggcggtg gtcatcgcct gctggctgga tgtggacgca
     5761 attacccgcg tgctgcttat cagctccgtg atgctggtga tgattgtgga aatcctcaat
     5821 agcgccatcg aagcagtggt tgaccgaatt ggctctgaat accatgagct ttccggacgc
     5881 gcaaaagata tgggatccgc cgcggtgctg attgccatta tcgtcgccgt gattacctgg
     5941 tgcatcctgt tatggtcgca ttttggataa cccttccaga tttcgataaa tctctggttt
     6001 attgtgcagt ttatggttcc aaaatcgcct tttgctgtat atactcacag tataactgta
     6061 tatacaccca gggggcggaa tgaaagcgtt aacggccagg caacaagagg tgtttgatct
     6121 catccgtgat cacatcagcc agacaggtat gccgccgacg cgtgcggaaa tcgcgcagcg
     6181 tttggggttc cgttccccaa acgcggctga agaacatctg aaggcgctgg cacgcaaagg
     6241 cgttattgaa attgtttccg gcgcatcacg cgggattcgt ctgttgcagg aagaggaaga
     6301 agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg cttctggcgc aacagcatat
     6361 tgaaggtcat tatcaggtcg atccttcctt gttcaagccg aatgctgatt tcctgctgcg
     6421 cgtcagcggg atgtcgatga aagatatcgg cattatggat ggcgacttgc tggcagtgca
     6481 taaaactcag gatgtacgta acggtcaggt cgttgtcgca cgtattgatg acgaagttac
     6541 cgttaagcgc ctgaaaaaac agggcaataa agtcgaactg ttgccagaaa atagcgagtt
     6601 taaaccaatt gtcgttgacc ttcgtcagca gagcttcacc attgaagggc tggcggttgg
     6661 ggttattcgc aacggcgact ggctgtaaca tatctctgag accgcgatgc cgcttggcgt
     6721 cgcggtttgt ttttcatctc tcttcatcag gcttgtctgc atggcattcc tcacttcatc
     6781 tgataaagca ctctggcatc tcgccttacc catgattttc tccaatatca ccgttccgtt
     6841 gctgggactg gtcgatacgg cggtaattgg tcatcttgat agtccggttt atttgggcgg
     6901 cgtggcggtt ggtgcaacgg cgaccagctt tctctttatg ctgttgctgt ttttacgcat
     6961 gagcaccacc gggctgactg cgcaggctta tggtgccaaa aatcctcagg cattagcccg
     7021 tgcgctggtg caaccgttgc tgttggcgtt gggggccggg gcgttaattg cgctgttgcg
     7081 tacgccgatt atcgatctgg cgctgcatat tgttggcggt agtgaagcgg tcctggaaca
     7141 ggcgcggcgc tttcttgaaa tccgctggtt aagcgctccg gcgtcgctgg cgaatctggt
     7201 attactcggt tggttactcg gcgtgcaata tgcccgtgcg ccagtaattt tgttagtggt
     7261 cggcaatatc ctcaacattg tgctggatgt ctggctggtg atggggctgc atatgaacgt
     7321 gcagggcgcg gcgctggcga cggttattgc ggaatatgca acattgctga ttggtctgct
     7381 aatggtgcgt aaaatcctca aactacgcgg aatttccggc gaaatgctga aaactgcctg
     7441 gcgaggaaac ttccgtcgct tgctggcgct taaccgcgat atcatgctgc gttcgctgtt
     7501 gttgcaactc tgtttcggcg cgatcaccgt acttggtgcg cgactgggga gtgacattat
     7561 cgctgttaac gcggttctga tgacgctact cacctttacc gcctatgcgc ttgatggttt
     7621 tgcctacgcg gttgaagcgc actccggtca ggcatacggt gcgcgcgacg gtagccagtt
     7681 gctggatgta tggcgggcag cgtgccgcca gtcggggatt gtagcgttac tattttcggt
     7741 ggtttatttg ctggcagggg aacacatcat tgcgttgctg acgtcgttaa cccagattca
     7801 gcagctggct gaccgctatc ttatctggca ggtgattttg ccgttggttg gcgtctggtg
     7861 ttatctgctg gacggcatgt ttataggcgc aacgcgtgcc gccgaaatgc gtaacagtat
     7921 ggcggtggcc gccgcaggtt ttgcgctgac gctccttacg ctgccgtggc tgggtaatca
     7981 tggtttgtgg ctggcattaa ccgtctttct ggcgttgcgc gggctttctc tggcggctat
     8041 ctggcggcgt cactggcgca acggtacctg gtttgccgca acgtgacggt taaaaattct
     8101 gaataaatta tcctaagcca aattgctgac tacacttaat ctcacgttca gaagaaaagt
     8161 gaacgtactc tcattcacaa cctaacgatg aggtcttgat tatgaataaa gatgaagccg
     8221 gcggtaactg gaaacagttt aaaggtaaag tgaaagagca atggggcaaa ctgaccgatg
     8281 atgatatgac gatcattgaa ggtaaacgtg atcaactggt cggtaaaatc caggaacgtt
     8341 atggttatca gaaagatcag gcagaaaaag aggtcgtgga ttgggaaacc cgcaatgaat
     8401 atcgctggta attaatccct cctgcccgac gtgtacaagg atgtacgccc tcttaacgcg
     8461 gtttcttttt cacctgcaca gagtgatcat gctggcactg ttcaggatga cgacacgctt
     8521 ccacttccac gcatgccgca cagagcccat gtgcttcaat cacattatgc cgcagggcaa
     8581 accccatttt tgccgccagc gtatgcataa tgtcttccac gccttctgca cactcttctt
     8641 tcaccgcgcc gcagcgatcg caaataaaca tggctgacgt atgcgtgggc tggtcgaaca
     8701 gatgacagag cacataactg ttggtggatt ccaccttatg cacaaaacct tgctcaagca
     8761 gaaaatccag cgcgcgataa accgttggcg gcttggcttg cggttcagct tcgcgcagta
     8821 aatcaagcag atcataagcg ctgatagcgc catcctgcag actcatcagg cgcaacactt
     8881 ccaggcgctg tggggtcagg cgcacattac gctgcgcgca gattttttca gcctgcgcta
     8941 ataactcctg cgttgtggtc ttttccatcg ggcacctcaa agtgggggtt aagaatgtcc
     9001 ttactttacc atgttccagg aaaaacaccg agaaccctta ttgttgccgt aatgttgatt
     9061 ttctgttttg taggtaaggt gttgtgttgc cttatcgtac cattatcaac acgataataa
     9121 ttaataattc atttttaaat aaattgcttc aagttaactc gctatttatt taactgggtt
     9181 taaacgcata cctgtagttc agaaattatg agcgattttt gatagtgacc agatggcaaa
     9241 agaattataa ggaggacacg gtacgacaaa aaaggtagat gaatcatgtt gaagataata
     9301 cccggtgcaa ct
//
LOCUS       DAZOYF010000157         9208 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZOYF010000157 DAZOYF010000000
VERSION     DAZOYF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.158"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="ACHUOF_003424"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="ACHUOF_003424"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR0612466.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="ACHUOF_003425"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="ACHUOF_003425"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR0612467.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="ACHUOF_003426"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="ACHUOF_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFR0612468.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="ACHUOF_003427"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="ACHUOF_003427"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR0612469.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="ACHUOF_003428"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="ACHUOF_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR0612470.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="ACHUOF_003429"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="ACHUOF_003429"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR0612471.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="ACHUOF_003430"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="ACHUOF_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFR0612472.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="ACHUOF_003431"
     CDS             complement(8064..9206)
                     /locus_tag="ACHUOF_003431"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFR0612473.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT         2545 a         2148 c         2034 g         2481 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatct
//
LOCUS       DAZOYF010000158         9186 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZOYF010000158 DAZOYF010000000
VERSION     DAZOYF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.159"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..923
                     /locus_tag="ACHUOF_003432"
     CDS             <1..923
                     /locus_tag="ACHUOF_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0612474.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHUOF_003433"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHUOF_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR0612475.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHUOF_003434"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHUOF_003434"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR0612476.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSNSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="ACHUOF_003435"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="ACHUOF_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR0612477.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="ACHUOF_003436"
     CDS             4446..4805
                     /locus_tag="ACHUOF_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR0612478.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHUOF_003437"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHUOF_003437"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR0612479.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="ACHUOF_003438"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="ACHUOF_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR0612480.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="ACHUOF_003439"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="ACHUOF_003439"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR0612481.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2102 a         2220 c         2483 g         2381 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatttgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg acgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa
     9121 atgcttgact ctgtagcggg aaagcgtatt atgtacaccc cgcgcagctg agaaaaagcg
     9181 aagcgg
//
LOCUS       DAZOYF010000159         9068 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZOYF010000159 DAZOYF010000000
VERSION     DAZOYF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.160"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            56..223
                     /locus_tag="ACHUOF_003440"
     CDS             56..223
                     /locus_tag="ACHUOF_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR0612482.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            213..815
                     /locus_tag="ACHUOF_003441"
     CDS             213..815
                     /locus_tag="ACHUOF_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR0612483.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            847..1410
                     /gene="pabA"
                     /locus_tag="ACHUOF_003442"
     CDS             847..1410
                     /gene="pabA"
                     /locus_tag="ACHUOF_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR0612484.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            1496..2716
                     /gene="argD"
                     /locus_tag="ACHUOF_003443"
                     /gene_synonym="dapC"
     CDS             1496..2716
                     /gene="argD"
                     /locus_tag="ACHUOF_003443"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR0612485.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(2783..4873)
                     /locus_tag="ACHUOF_003444"
     CDS             complement(2783..4873)
                     /locus_tag="ACHUOF_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR0612486.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(4924..5556)
                     /gene="crp"
                     /locus_tag="ACHUOF_003445"
     CDS             complement(4924..5556)
                     /gene="crp"
                     /locus_tag="ACHUOF_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR0612487.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            5858..6262
                     /locus_tag="ACHUOF_003446"
     CDS             5858..6262
                     /locus_tag="ACHUOF_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR0612488.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            complement(6317..7186)
                     /locus_tag="ACHUOF_003447"
     CDS             complement(6317..7186)
                     /locus_tag="ACHUOF_003447"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR0612489.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(7240..7458)
                     /locus_tag="ACHUOF_003448"
     CDS             complement(7240..7458)
                     /locus_tag="ACHUOF_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR0612490.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            complement(7452..8474)
                     /locus_tag="ACHUOF_003449"
     CDS             complement(7452..8474)
                     /locus_tag="ACHUOF_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0612491.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            complement(8474..>9068)
                     /locus_tag="ACHUOF_003450"
     CDS             complement(8474..>9068)
                     /locus_tag="ACHUOF_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0612492.1"
                     /translation="LALIVWQRPNLLLLDEPTNHLDLDMRQALTEALIEFEGALVVVS
                     HDRHLLRSTTDDLYLVHDRKVEPFDGDLEDYQQWLSDVQKQENQTDEAPKENANSAQA
                     RKDQKRREAELRAQTQPLRKEIARLEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELT
                     ACLQQQASAKSGLEECEMAWLEAQEQLEQMLLEGQSN"
BASE COUNT         2063 a         2312 c         2465 g         2228 t
ORIGIN      
        1 acccggattt gccgcttata cttgtggcaa atggacacgt tcagggaggc atcaagtgaa
       61 gaaactcacc gataagcaaa agtcccgtct ctgggagctt cagcgtaatc gtaatttcca
      121 ggccagtcgc cgtcttgaag gcgtcgagat gcctttagtc actcttactg ccgcagaggc
      181 tttagcgcgc cttgaagagc tgaggaggca ctatgagcga taaattcggc gaagggcgcg
      241 atctgtatct ttatcctggc cttgatatca tgcgtaaccg gctgaacatc cgccagcagc
      301 agcggctgga acaggccgct tacgaaatga cggcgctgcg tgctgcgacc attgagcttg
      361 gtccgctggt gcgcggttta ccgcatttgc gcactatcca tcgccagctg taccaggata
      421 ttttcgactg ggcagggcaa ctgcgtgaag ttgatattta tcagggcgat acgccgttct
      481 gccactttgc ttatatcgaa aaagagggca atgccctgat gcaggatctg gaggaagaag
      541 gttatctggt tggcctggag aaagcgaagt tcgtcgagcg gctggcgcat tactattgtg
      601 aaatcaacgt gctgcatccc ttccgggtgg gaagtggtct ggcacagcgg atcttcttcg
      661 agcaactggc gattcatgcc ggatatcaac tgagctggca gggtatcgaa aaagaggcct
      721 ggaatcaggc aaatcagagt ggggcaatgg gggatctcac cgcactgcag atgatattta
      781 gcaaagtggt aagcgaagcc ggggaatctg agtaaaatag cgcggttctt tcgtaccgga
      841 gccgccatga tcctgcttat agataactac gattctttta cctggaacct ctaccagtac
      901 ttttgtgaac tgggggcgga tgtgctggtt aagcgcaacg atgcgttgac gctggcggat
      961 atcgacgccc ttaaaccaca aaaaattgtc atctcacctg gcccctgtac gccagatgaa
     1021 gccgggatct cccttgacgt tattcgccac tatgccgggc gcttgccgat tcttggcgtc
     1081 tgcctcggtc atcaggcaat ggcgcaggca tttggcggca aagttgtgcg cgccgcaaag
     1141 gtcatgcacg gcaaaacctc gccgattaca catagcggtg agggcgtatt tcgggggctg
     1201 gcaaatccac ttaccgtgac acgctaccat tcgctggtgg tggaacctga ctcattacca
     1261 gcgtgctttg acgtgacggc ctggagcgaa acccgcgaga ttatggggat tcgccatcgc
     1321 cagtgggatc tggaaggtgt gcagttccat ccagaaagta ttcttagcga acaaggacat
     1381 caactgctgg ctaatttcct gcatctctga tttccgattg ccattcagtg attttttatg
     1441 catattttgt gattataatt tcacatttat ttatgcgtaa cagggtgatc atgagatggc
     1501 aattgaacaa acagcaatta cacgcgcgac tttcgatgaa gtgatcctgc cgatttatgc
     1561 tccggcagag tttattccgg taaaaggtca gggcagccga atctgggatc agcaaggcaa
     1621 ggagtatgtc gatttcgcgg gtggcattgc agttacggcg ttgggccatt gccatcctgc
     1681 gctggtgaac gcgttaaaaa cccagggcga aactctgtgg catatcagta acgttttcac
     1741 caatgaaccg gcgctgcgtc ttgggcgcaa actgattgag gcgacgtttg ccgaacgcgt
     1801 ggtgtttatg aactccggca cggaagctaa cgaaaccgcc tttaaactgg cacgccatta
     1861 cgcctgtgtg cgtcatagcc cgttcaaaac caaaattatt gccttccata acgcttttca
     1921 tggtcgctcg ctgtttaccg tttcggtggg tgggcagcca aaatattccg acggctttgg
     1981 gccgaaaccg gcagacatca tccacgttcc ctttaacgat ctccatgcag tgaaagcggt
     2041 gatggatgat cacacctgtg cggtggtggt tgagccgatc cagggcgagg gcggtgtgac
     2101 ggcagcgacg ccagagtttt tgcagggctt gcgcgagctg tgcgatcaac atcaggcatt
     2161 attggtgttt gatgaagtgc agtgcgggat ggggcggact ggcgatttgt ttgcttacag
     2221 gtactacggt gtgacgccgg atattctgac ttctgcgaaa gcgttaggcg gcggcttccc
     2281 gattagcgcc atgctgacca cggcggaaat tgcttctgcg tttcatcctg gttctcacgg
     2341 ttccacctac ggcggtaatc ctctggcctg tgcagtagcg ggcgcggcgt ttgatatcat
     2401 caatacccct gaagtgctgg aaggcattca ggcgaaacgc cagcgttttg ttgaccattt
     2461 gcagaagatc gatcagcagt acgatgtgtt tagcgatatt cgcggtatgg ggctgttaat
     2521 tggcgcggag ctgaaaccac actacaaagg tcgggcgcgt gatttcctgt atgcgggtgc
     2581 agaggttggc gtaatggtgc tgaatgccgg accggatgtg atgcgtttag caccgtcgct
     2641 ggtggtggaa gatgcggata tcgatgaagg gatgcaacgt ttcgcccacg cggtggcgaa
     2701 ggtggttggg gcgtaaataa gacgtatcag gcaaaatatg tcggatgcgg cgtgaatgcc
     2761 ttatccgatg gtttagcctg tattacgcct tcgaatcccg caacttgcga ctcagccaaa
     2821 tcccgtgatg cggtcgctga cgccatgcca tcgacgaaat ggtgtgcatg gtgttcagat
     2881 ggccaataac ccgctgtaaa tgctgctcca gcgtacctgc cgcgccttcg tgcggctgca
     2941 tctccggcgc atccatgata ttggcatcgc cagaactacc cggttcgtca tactccagtc
     3001 gctgctgaca acgctgaatg gcgatttcac aagactgtaa atactcttgt gccagttcag
     3061 gtggcaatgc ccggtgttcc cgcgccagcg tggtcatggc attaatatgc tcaacaataa
     3121 actggctgtg cgttacccac agtttcatat ctgccagata atggctgttg aatgccggtt
     3181 cctgcatcgc ctgattcaat gagttataca gagtgttatg cgcctgattt acccgcattc
     3241 tctgccaggc cagtggcgtt ggttgcgggt cctcgctcag aatcaagcga atcgcttcct
     3301 gataggcttc taaagcatca tgggcgtttt tacgcaataa cccgctctgc cactgcggcc
     3361 acagccagac ggtaccaccg aaagcaatca aacaaccaat aatggtatcg ataagacgtg
     3421 gaaggatgta ctgctcaccg ttcagccaca acagttgcag ggtatacact gcggtaatag
     3481 taaaaccgac cgttgcccag ccgtagtttt tgcgcaatat caggtagctg gcgagggtgg
     3541 taatcagcat caacgtcagg gtgtaacttt cgggaatttt aaagtgcagc gctacgcccg
     3601 caatgattaa cccgaccacg gttcccacgg agcgattcac aatcctcaga cgggttgcgc
     3661 catagccatt ttgtgtcacc agcaataccg tcatcaggat ccagtacgac ttcggcagat
     3721 gcagcacggt gcccatcagg ctggcaacgc ttaacatcac actgagtcgt ccggcattgc
     3781 gtagcgccgg agactttagt gacagataac ttttcagtgc cggaagcaac ggcattcgcc
     3841 gctgtttatc ggccagtaag tcacgggcat agagcggttt ttgggtgcgc agcacgcggg
     3901 cgatgcggct gaaatgccag tagcagaatt gcccaaccgg attatccgga tgctggcggg
     3961 cgattttttc cagtgcgcca atttgctttt ccatcgtaaa acgcgttggc aggcgatggt
     4021 aaagaatgtc atcagccagc acgcgcaggc gagcggcgac ggtttgcgca ttccagcgga
     4081 taacttcttc cgcatggcta cgctcgacca gtttttgcac ctcttccggc tgatgcaaac
     4141 tgaccgaaat atgttcctgc aaatccagcg cctcctggaa aatacgcagc atccgcttgt
     4201 aatcggtatt gttttgcgcg gaaagcatat gcatttgctg atagcactga gtaattaaat
     4261 cgaccgcttt ttgctggcgc accagcagcg gtggcagcgc tttttcagga tcggtgtgct
     4321 gggtaagcag gctgtatttg gcttcacaat aatctgccag ttcacggtac agcagactta
     4381 gtgactcgcg cagcggttgt tcgcgccaga tccagaacca aaaccagtta aacaatccgt
     4441 accagagagt gcccaacgca tagatgagca atggttccca gaccggcatg tatcccgcca
     4501 gactgagggt aaaaatggcg gcgagcagcg atgcaggaag caattttgcg tgtaatggcc
     4561 ccagctcagc agtgacgcca agtaccagcg ttaatccggt cagcaaaaag ggcaggggaa
     4621 catcttttac cagtagcagc tgtgtcagta agctacaggt ggcaaacagc gacgcaccaa
     4681 tgattaagtg tttgaaaaaa cgtttatgag gcgtatcaag gcccgcaata ttgcaacagg
     4741 caggaacgag ggagaagagc agaccgaatc gtaattcgcc aagcattaac ccaacggcca
     4801 cgggcaaaca tagcaccagc gtttgtcgaa gtgcatagtt gatatcgggg tgataaatca
     4861 gtctgcgcca catcggggga aacaaaatgg cgcgctacca ggtaacgcgc cactccgacg
     4921 ggattaacga gtgccgtaaa cgacgatggt tttaccgtgt gcggagatca ggttctgatc
     4981 ttccagcatc ttcagaatgc gtcccacggt ttcacgagaa cagccgacaa tctgaccgat
     5041 ttcctgacgg gtaattttga tttgcatacc gtccgggtga gtcatagcat ctggttgttt
     5101 tgccaggttc agcagagtct gtgcaatgcg gcccgtcacg tcgaggaacg ccaggttgcc
     5161 cactttctct gaagtgactt gcagacgacg cgccatctgt gcagacagac gcatcagaat
     5221 gtccgggttt acctgaatca attggcgaaa ttttttgtac gaaatttcag ccacttcaca
     5281 ggcggttttc gcacgtaccc atgcgctacg ttcctggccc tcttcaaaca ggcccagttc
     5341 gccaataaaa tcaccctgat tcagatagga gaggatcatt tctttaccct cttcgtcttt
     5401 gatcagcact gccacagagc ctttaacgat gtagtacagc gtttccgctt tttcaccctg
     5461 gtgaataagc gtgctcttgg atgggtactt atgaatgtgg caatgagaca agaaccattc
     5521 gagagtcggg tctgtttgcg gtttgccaag caccatgcgc ggttatcctc tgttataagc
     5581 tttctccaga gccagataac gccgctgtct ctggattgcc gaaatatgct tcccgctacc
     5641 tgggaagggg ctatcaactg tactgcacgg taatgtgacg tcctttgcat acatgcagta
     5701 catcaatgta ttactgtagc atcctgactg ttttagcata gctttcgctt tgtgtctcct
     5761 ggtgtctcgc ttcagcatga cccaggtcgc cttccgttgc gcgatttggt tagtacgcgt
     5821 actctgtcag gaaaattgac gcagtggagt agcaaaaatg caagcgcgag tgaagtgggt
     5881 cgaagggtta acttttctgg gcgaatccgc ctctggtcac cagattttaa tggacggcaa
     5941 ctcaggcgat aaagcaccaa gtccgatgga aatggtgttg atggcggcgg gtggttgcag
     6001 tgccatcgat gtggtttcga tcctgcaaaa agggcgtcag gatgtggtcg attgtgaagt
     6061 aaaattgacc tctgaacgcc gcgaagaggc accacgcctg tttacgcaca ttaatctgca
     6121 ttttatcgtc accggtcgcg acctgaaaga cgcagcggtt gcgcgtgcgg ttgatctctc
     6181 tgccgagaaa tattgttcag tggcgttgat gctggaaaaa gcggtgaata ttactcactc
     6241 gtatgaagtg gttgccgcgt gacttaacta tcccgttgtg aagccggata gtattttatc
     6301 cggcataata gcaaagttac tcgattttct ttccttccat caatcgttgc accagcggca
     6361 gcataattaa ttccattgcc agccccattt tgccgccagg taccactaac gtattgatgt
     6421 gggaaatgaa tgagccttgc aacatcgcca gcagccaagg gaaatcgatc ccttccagat
     6481 tacgaaaatg gatcaccaca aagctttcat ccagcgacgg gatacctttt gccgcgaacg
     6541 ggtttgaagt gtcgacggtg ggaacgcgct ggaagttaag atgggtgcgg gaaaactgcg
     6601 gtgtgatgta gttgatatag tcttccattg aacgcactac cgagtccatc actgcttctc
     6661 gtgagtgccc gcgctcgctg gtgtcgcgga tcagtttttg aatccactca aggttaacga
     6721 taggcactac gccgaccagt aagtccacat gctgcgcaac gttatgctgt ggcgtgacta
     6781 cgccgccgtg taaaccttca taaaacagta catcagtggg ttctggtaaa ggttgccagg
     6841 gggtgaatgt tcccggtacc tgattccagg gtacggcttc gtcgtaggta tgcagatatt
     6901 tgcgagattt ccctttgccg ctctgaccgt attcaatgaa ggtttgttcc agcaggccga
     6961 agtcgttcgc ttcggggccg aagtagctga tatgccgccc ggcgtcgcgc gctttgcgga
     7021 tcgccatgtc catttccggg cgggtgtaac ggtgaaaact gtcgccttcc acctcagctg
     7081 catgcagatt taactgcgcg aatattttac gaaacgcgag gctggtggtg gtggtccccg
     7141 cgccgctgga tcctgttacc gcaatgaccg gatgtttggc agacatagta actccatgac
     7201 ttagctatca ctattatctc gtccgccgac ggttgcaggt tattcgcgaa actgactgcg
     7261 cggcataata ttgaccgttt cgtgcagctc cgaccatacc agcaccgctt ctccgcactg
     7321 tagctggcgt ttgacgtcgg cgactttctg ttcaagtgta cgctcatgtt caccataatc
     7381 ggtgccttca cgtaacacaa agctttcaat taaattttcc agcgtttcgg gggagaggtc
     7441 ttgccacgga atcaacatga tttcgcctcc agatacgttg ttaaccagtc aggaatgcgt
     7501 gactccagcc acatttgcgg atgaagtaat gtaccgccaa taaagccaac atgaccgcca
     7561 tgttcagtca gttgatactc cacctgcgag gggagacttt ccggttttgg gatcacctga
     7621 tgatccataa acggatcgtc tttggcgtga ataatcagcg tcggtttggc gatccggttc
     7681 agcattggca tggcgctaca ctgacgataa tagtcgatag cgtcggcgta gccgtgaatt
     7741 ctggcggtga ttagatcgtc aaattcacgg atgcgacgta ccgattttaa ctgcgcgaga
     7801 ttaatcggca gcgttccggg gtaggctgcc agcttgcgcg cggcattggc ttttaacagg
     7861 ttcagcaagt aacgctgata aacgcgggaa aagccctttt ccatatgata gctacaggct
     7921 tccagcataa acggcgcaga gacaatcacc gccgcatcaa ccgggagatc attgccttcc
     7981 tttgccagca aacaggccag catattaccg ccgagcgaat agccgacggc agccgttggc
     8041 gcatgaccaa attcgcgttg cagccagcgt aaaaaccaac tggcgtcttc ggtttcgccc
     8101 gaatggtaaa tgcggtgcat acggtttggt tcaccgctgc atccgcgaaa atgcatcacc
     8161 acgcccagcc agccgcgctt ttgcgccgcc tcaaccagac cgtgggcgta agggctattg
     8221 agactgcctt ccagcccgtg aaacaccact aaacgcggtt tatgtttcgc ctgtgcgggg
     8281 tcttcactcc acgcgagatc gacaaaatcg ccgtcgggca actccagccg ctgccagtac
     8341 ggggtgaatt tcacctgacg acgaaacaaa cgcggcagca tagtttgtag atgacaattg
     8401 ctaaagccgc gcataggggt gaattcagca ctgctgctaa attcattggc atcggtcgtc
     8461 gttatctgcg ccatcagttg ctttggcctt ccagcagcat ctgctcaagc tgctcctggg
     8521 cttccagcca cgccatttcg cactcttcca ggccggattt ggcgctggct tgctgttgca
     8581 ggcaggcggt caactccgct ttacggctct ggtcatacag ttcgctgtcg ccgagtttct
     8641 cttccgcctg cgccagttgc gcgttcagct tctccatctc tttttccaga cgggcaatct
     8701 ctttacgcag cggctgggtt tgcgcacgca gctccgcttc ccgacgcttc tgatctttac
     8761 gtgcctgggc gctgttcgcg ttctcttttg gcgcttcgtc ggtctggttt tcctgctttt
     8821 gtacgtcgct caaccactgt tgataatctt ccagatcgcc gtcgaacggt tcgactttac
     8881 gatcgtgaac caggtagaga tcgtcagtgg tggaacgcag caaatgacgg tcgtgcgaaa
     8941 cgacaaccag cgcgccttca aactcgatta atgcttcggt gagtgcctga cgcatgtcga
     9001 ggtcaaggtg gttagtcggt tcgtcgagca gcagcagatt cggacgctgc cagacaatta
     9061 atgccagc
//
LOCUS       DAZOYF010000160         9029 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZOYF010000160 DAZOYF010000000
VERSION     DAZOYF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.161"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHUOF_003451"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHUOF_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR0612493.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHUOF_003452"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHUOF_003452"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR0612494.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHUOF_003453"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHUOF_003453"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR0612495.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHUOF_003454"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHUOF_003454"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR0612496.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVQ
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="ACHUOF_003455"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="ACHUOF_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR0612497.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /gene="smg"
                     /locus_tag="ACHUOF_003456"
     CDS             5562..6035
                     /gene="smg"
                     /locus_tag="ACHUOF_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR0612498.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="ACHUOF_003457"
     CDS             6064..6606
                     /locus_tag="ACHUOF_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR0612499.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHUOF_003458"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHUOF_003458"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR0612500.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="ACHUOF_003459"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="ACHUOF_003459"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR0612501.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="ACHUOF_003460"
     CDS             8003..8260
                     /locus_tag="ACHUOF_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR0612502.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="ACHUOF_003461"
     CDS             complement(8236..8790)
                     /locus_tag="ACHUOF_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR0612503.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2259 a         2191 c         2251 g         2328 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gtgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgctgca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagag
//
LOCUS       DAZOYF010000161         8922 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZOYF010000161 DAZOYF010000000
VERSION     DAZOYF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.162"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            78..2262
                     /gene="espL4"
                     /locus_tag="ACHUOF_003462"
                     /pseudo
     CDS             78..2262
                     /gene="espL4"
                     /locus_tag="ACHUOF_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2231..3968)
                     /gene="aceK"
                     /locus_tag="ACHUOF_003463"
                     /pseudo
     CDS             complement(2231..3968)
                     /gene="aceK"
                     /locus_tag="ACHUOF_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4152..5456)
                     /gene="aceA"
                     /locus_tag="ACHUOF_003464"
     CDS             complement(4152..5456)
                     /gene="aceA"
                     /locus_tag="ACHUOF_003464"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFR0612504.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5486..7087)
                     /gene="aceB"
                     /locus_tag="ACHUOF_003465"
                     /pseudo
     CDS             complement(5486..7087)
                     /gene="aceB"
                     /locus_tag="ACHUOF_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7356..8285)
                     /gene="metA"
                     /locus_tag="ACHUOF_003466"
     CDS             complement(7356..8285)
                     /gene="metA"
                     /locus_tag="ACHUOF_003466"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR0612505.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8442..8885
                     /locus_tag="ACHUOF_003467"
     CDS             8442..8885
                     /locus_tag="ACHUOF_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR0612506.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2337 a         2151 c         2158 g         2276 t
ORIGIN      
        1 tagttgtaat catattaaat tttttcactg ttctgatagt taaaattcaa gacatcaata
       61 aacaatgaga tatttaaatg attactcgta ttcctcgtag ttctttctct gcaaatatta
      121 ataatacagc ccagacaaat gaacaccaaa ccctgagtga attgttttat aaagaactcg
      181 aggataaatt ttctggcaag gagctggcga cgcctctatt aaaaagcttc tcagagaact
      241 gtagacaaaa tgttcgtcat atctttagca acaaggattt tgtcattaaa ttttccacgt
      301 ccgtcttaca agctgataag aaagaaatta cgatgattaa taaaaacgaa aacacgacac
      361 tcactcaaac cattgctaca atatttgaag aatacctaat ggaaatttta cctcaacgct
      421 cagacactct tgataaacaa gaattaaacc aaaaatcaga tagaaaagaa aaagaattcc
      481 caagaattaa acttaatggt caatgttatt tcccggggcg ccccaaaacc gtatagtata
      541 ccgacacatt gctgcacaat atattaatga tatttatcag aatgttgatt acaaacccca
      601 tcaagatgat tactcttcag ctgaaaaatt tctcactcac ttcaacaaaa aatgcaaaaa
      661 ccagactttg gcgttgattt ccagccgtcc tgaggggcgt tgcgttgctg cctgcggtga
      721 tttcgggcta gttatgaaag catattttga caagatggaa tcaaatggca tcagtgttat
      781 ggcagccata ttactggtgg ataaccatgc tttgacggtc cggctaagaa taaagaacac
      841 aactgaagga tgtacccatt acgtggtttc ggtttatgat cctaatgtaa ctaacgataa
      901 aataagaatt atgagcgaaa gcaaagagga tattaaacac tattctctga tggattttat
      961 gaatgtagat tatagcctcc tgaaatggtc aaatgatcat gttattaacc aatctgttgc
     1021 aataattcca gcacttccga aagaacagct attgatgtta aaaggaactg tggatgaaat
     1081 aacccctcca ttatcaccag caacgatgaa tttgctaatg gcaattggtc agaatcacca
     1141 acttacgcaa ctgatgattc agctccagaa aatgccagaa ctacatagaa cagaaatgtt
     1201 gactgcctat aatagtatta acctccccgg tttatatttg gctataaatt atggcaatgc
     1261 ggatatcgtt gagactattt tcaattcatt gtcagaaaca ggatatgaag ggttactctc
     1321 gaaaaaaaat ctcatgcata ttctggaggc aaaagataaa aatggttttt ctggattatt
     1381 tttagcgata tcacgtaagg ataaaaatgt tgtaacctcg attctgaacg tcttacctaa
     1441 actggccgca acacatcatt tagataatga acaggtgtat aaattcctga gtgccaaaaa
     1501 tagaacgtcc agccatgttt tataccatgt tatggcgaat ggtgatgccg acatgctgaa
     1561 aatttttttg gtcgcgttac ctttgttaat tcgcacatgt catttgacta aagaacaggt
     1621 actcgatctc ctgaaggcaa aggattttta tggttgccca agactatacc tggcgatgca
     1681 aaatggacat agcgatatcg tgaaagttat tctcgaagca ttgccctgtc tggcccagga
     1741 aattaacatt tcagcttccg atattgtaga tcttctgacc gctaaaagtc ttgcgcgcga
     1801 cacgggtttg tttatggcca tgcagcgcgg acatatgaac gttattaata ctatttttaa
     1861 cgcattaccc actcttttta atacgtttaa attcgataaa aaaatatgaa gcccctcctc
     1921 ctggcaaata attctaatga atatcccggt ttgttttcag cgatacagca taaacaacaa
     1981 aacgttgtag agacggttta tcttgcttta tctgaccatg cacgcctgtt tggatttacc
     2041 gctgaagata ttatggattt ttggcaacac aaagcgccac aaaaatactc tgcctttgag
     2101 ttggcttttg aatttggtca ccgggttatt gctgaattaa tccttaatac attaaataag
     2161 atggctgaaa gctttggctt tacggataac cctcgataca ttgcggagaa aaattatatg
     2221 gaagctttac tcaaaaaagc atctccccat accgtacgct aaatctttgc ctgcgccgat
     2281 acgcataaac atcttccaca tgcccttcac gtatgcggtt ttgtagtgcg cgccagtaat
     2341 cagcgcggaa caggtcggcg tgcatctctt caaacagcgg accaatacgc gggtcggcgc
     2401 atagccagtg gcgaaactct tccgggaaaa catcgcccgg cgagacgctg taccacggtt
     2461 cgctggcaag ttcgtcttcc ggatagcgcg gcggcgggat gtcgcggaaa ttcacttccg
     2521 tcatgtagca aatttcatcg taatcataaa aaaccacacg cccgtgacgg gtgacaccga
     2581 agtttttaaa cagcatgtcg ccagggaaaa tgttagcagc ggcaagctgg cgaatagcgt
     2641 taccgtattc ttcaatggcg tcgcgcaact gctgaccttc cacttgttcc agccagatat
     2701 tgagcggcac catccgccgc tcgattatag agatggcgaa ttacaatctg ctcaccaaga
     2761 tcggtgattt tttccgctgc ttcctggagc agtaattcca ttaataccgg ggaaatatgc
     2821 cgcttctcca gcacaaagtt ttcaaactcc tgggtgtccg ccattcggcc cacacggtcg
     2881 tgctctttca ccagttgata gcaggcacga acgtgagcgg cagacatctc tttctgcggc
     2941 gcgaacttgt ctttgatgac tttgaatacc cgatcaaagc ccggcagcgt aaacaccaac
     3001 atcaccattc cacgaatacc cggcgcttca atgaactgct cattacagcc ctgtagataa
     3061 acgagatatt cgcggtagct ttcggttttg gcgtgcttct ggcagccgat agccatatac
     3121 aattcagcag tggttttacc tggcagaatt tcccgtagcc actcgaccag cgctgcgggc
     3181 agcggtgcat aaaccataaa ataagaacgc gcaaagccaa aaacaatgct cgcttcggcg
     3241 gtcgtcgtca ggcaggtatc aataaataac tcgccgtcgt ccgtctggtg gatcggcagt
     3301 aagaagggca acgtgccgga aggcgtgatc agtttgccca ccagccaggc ggctttattg
     3361 cggtaaaaca gttcgttcgc cacctgtaaa tgactttccg cgaggttgtc tgtccccagc
     3421 gtttccgtca gatggcgaat gatgtaatga atgtcacggc ttttattctg ccagcgcagg
     3481 cgcagcggca ggtcgctgat aacgcgcatc agtagagatt cccagccgtg atcggggtga
     3541 aagtctttcg ccagcgggcg gggaatggta cgaaagcggc gctctggctg agagctaaag
     3601 ataaaaagcc gctcgggagt aagcgagcgg tggtcaaata accgacagta cacggagtta
     3661 aaaaagctct ccgcaatctc gaagcgcggg taatccggca acagccgcgt gtaatgctct
     3721 ttaacgcgca gtaaaaatgc cgcgtccgta ctttggccgt tagtaatgca gcgcagttgc
     3781 tccacgacca gaccaacgtg atgatcgtaa agatggatac ggtttttcat cgcctgctgg
     3841 acagcatgcc agtcggcctg ttcgaaacgc tgctgcgccc cggaagtcac ttcgaggaat
     3901 cgaccatact gagcgtcgaa gccttgcaaa atggtttgag caatcagtaa ttccaggcca
     3961 cgcggcattt agcgccctca tcaggagcag agaattgccg gatgcggcgt aaacgcctta
     4021 tccagcctac gttcggcaac ggttgtaggc atgataagac gcgcaagcgt cgcatcaggc
     4081 atcgtgcacc gattgccgga tgcggcgtaa atgccttatc cggcctacag tcagcaacgt
     4141 ttgtaggttg cttagaactg cgattcttca gtggagccag tcagcgcggt gactgaagac
     4201 gtgccgccct gaataatagt cgtcacttta tcgaagtaac ctgtacccac ttcctgctgg
     4261 tgagatacga aggtatagcc atctttcgcg gcggcaaatt ccggctgctg cactttctca
     4321 acatagtgct tcataccttc gccctgcgca taagcgtttg ccaggtcgaa catgttaaac
     4381 cacatgctgt ggatacctgc tagggtgatg aactggaact tgtagcccat atccgacagc
     4441 tgctgctgga agctggcaat agttttgtcg tcgaggtttt tctgccagtt gaacgacggc
     4501 gagcagttat aagccagcag tttgcccgga tatttcgcgt ggatagcttg tgcaaagcga
     4561 cgcgccagtt ccagatccgg cgtggaggtt tcacaccaga ccaggtcagc atatggcgca
     4621 tacgccaggc cacggctgat cgcttgctca atgcccgcat gagtacggaa gaagccttca
     4681 ctggtacgct cgccggtaat aaattcgctg tcatacgggt cgcaatctga ggtgatcaga
     4741 tccgccgcat cagcatcggt acgtgcaacc agcagggttg gaacgcccgt cacgtcagct
     4801 gccagacgcg ccgcgaccag tttctgaata gcttcctgag ttggcactaa aactttgccg
     4861 cccatgtgac cgcatttctt cactgacgcc agctgatctt cgaagtgaac tgccgcagca
     4921 ccggcttcaa tcatcgcttt catcagttca aaggcattca ggacaccgcc aaaaccggct
     4981 tccgcatcgg caacgatcgg caggaagtaa tcgacatagc gcggatcgcc cggctcaatg
     5041 cccacggacc attggatctg atcggcacga cggaaggtgt tgttgatccg ctccaccaca
     5101 gctggcaccg agtttgccgg atagagcgac tgatccggat acatgctggc cgccaggtta
     5161 gcgtccgccg ctacctgcca tcccgacaga tagactgctt caatacccgc tttcgcctgt
     5221 tgcagcgcct gaccgccagt cagtgcaccg aggttgttga tgtagccttt tttcgactca
     5281 ccgtgcagca gacgccacat tttcgctgcg cccagttgcg ccagcgtgca ttcaggattg
     5341 actgaaccgc gtaatttcac cacatcttcc gcactgtatg ggcgagtaat gccttcccaa
     5401 cgcggttgag tccactcttt ctgtaattct tcaatttgtt gtgtacgggt tttcatgtgc
     5461 agatgctcca tagttatgtg gtggtttacg ctaacaggcg gtagcctggc agggtcagga
     5521 aatcaattaa ctcatcggaa gtggtgatct gttccatcaa gcgtgcggca tcgtcaaaac
     5581 ggccctggga gaaacgttct tcgcccagtt cgctggcaat gactttcatc tcttcgccca
     5641 gcatctggcg gaacaaggct ttggtcaccg gtttgccatt gctcaacgtt ttttgatgat
     5701 ggatccactg ccagatcgag gtacgggaaa tttcagccgt cgccgcatct tccatcaggc
     5761 cataaatcgg cacgcagccg ttgccggaga tccacgcttc gatgtactgc acagccacgc
     5821 gaatgttagc gcgcatacct tcttcggtgc gttcaccatc acaaggtgcc agcagctgat
     5881 cggcagtaat cggcgcgtct tgttcgcgca atacttcaag ctgattttta cgggagccga
     5941 gaatgtcgtt gaataccgcc attgccgtgt ccgcaaggcc tgggtgagcg atccatgtgc
     6001 catcgtgacc gttattggct tccagcgctt tatccgcttt tactttgttg agcacctggt
     6061 tattacgctc ttcatctttg ctcggaataa acgcggccat gccgcccatc gcaaaagcac
     6121 cgcgtttatg gcaggtttta atcagcaggc gtgagtaagc attcaggaat ggtttatcca
     6181 tcgtcacggc ctgtctgtct ggcaggacac gatcgggata gtttttcaac gttttgatat
     6241 agctgaagat gtaatcccag cgaccgcagt tcagaccaac aatatggtcg cgcagcgcgt
     6301 gaaggatttc gtccatctgg aacacggcgg gcagcgtttc aatcagcaac gtcgccttga
     6361 tggtgccgcg cggcagatta aagcgatctt ctgcatagct gaagacttcg ctccaccagg
     6421 ccgcttcctg ctaggactgg gttttcggca gatagaaata gggaccgctg ccctttgcca
     6481 acagtgcctg atagttgtgg aagaaataga gcgcaaaatc aaacaggcta ccggggattg
     6541 cctcaccacg ccaggtgaca tgtttttccg gcaagtgcag accgcgtacc cgacaaatca
     6601 aaaccgctgg attgggcttg agctgataaa ttttgcctgc ttcattggta tagctgatgg
     6661 taccgttaac cgcatcgcgc aggttaattt gcccgtcgat cactttgttc cagtctggtg
     6721 ccagtgaatc ttcgaaatcg gccataaaga ctttcacatt ggcgttcagt gcgttgatca
     6781 ccatcttgcg ctctaccggg ccagttatct ctacgcggcg gtcttctaag tccgcaggaa
     6841 tcccgcgaat tttccaatca gcatcgcgaa tggaagccgt ttccgaaata aaatcaggca
     6901 acgttccgtt atcaatatct tgctgctgct gaatgcgcgc tgccagaagt ttattgcgtt
     6961 gtggcgtaaa atgcgtcacc agctcagtca gaaattctac cgcttcggca gtaagaattt
     7021 gcttctcctg ctcgccatac ggccttgtga aagccagttc atcggttgtt gttgcctgtt
     7081 cagtcatcgt gcagctcctc gtcatggatc cgaaaacttc ccctctgtga acgaaggaac
     7141 gttgtgcgct tttcgttcag cacaactaag actactcatt taaaatgcaa aatcaaaaac
     7201 aatttccatt ttaattaaaa atacttgata acttattgat aacaatatga ataaaattta
     7261 ttcacttgct gaggtgcgtt tcggaaataa aaaaggcacc cgaaggtgcc tgaggtaagg
     7321 tgctgaatcg cttaacgatc gactatcaca gaagattaat ccagcgttgg attcatgtgc
     7381 cgtagatcgt atggcgtgat ctgatagacg taatagttga gccagttggt aaacagcaaa
     7441 ttaccgtgac tacgccagct cgctcgcggt ttgttttgcg gatcgttgtg cgggaaatag
     7501 ttatacggta catccgggtc tagtccggct tccacatcgc gtaaatattc ctgcgccagc
     7561 gtttgcgcat catattcggg atggcccgtc acaaaggcaa tgcgcttatc tttgctggca
     7621 aacagatatg catccccttc ttccgtctct gccagaattt ccagatcggt gtaatcacga
     7681 atcaacgctg ccggaaagtc agcatagcgc gaatgcggtg ccaggaatga atcatcaaag
     7741 ccacgcgtca gaagcgcatg aggatggaga atatgatgct cgtaaacgcc agagagtttg
     7801 tcggtgcgag tttgcttagg aatgccgtag aggatattga gcgcggcctg taccgcccag
     7861 cagacaaaca gcgtcgaggt gacgtgatct ttcgaccact ccagcacctg tttgatctgc
     7921 ggccagtaag cgacatcatt aaactccacc aggcccagcg gcgcaccggt tacaatcaga
     7981 ccgtcaaagt tctgttcctg aatatcttca aagttacagt agaagttgtt cagatgctct
     8041 gcgggcgtgt tgcgcgattc acgggaatcg atgcgcaaca gctgaatatc gacctgcaaa
     8101 ggtgagtttg aaagcaggcg cagaaactga ttttcagttt caatcttctt cggcatcagg
     8161 ttaaggatca gaaccttaag tggacgaatt tcctgaccag acgcacgaga agttgtcatc
     8221 acaaagacgt tttcttcacg caagaaattg acggcgggta gctcgtccgg cacacgaatc
     8281 ggcataacct gattacctca ctacatacgc ttatacgttt agacatcgag atagctgaag
     8341 ataaccagaa aatttgccaa tgtcgagcct gcatgttgaa ggtgagaaag tttcaaggaa
     8401 aaggctacgt tagaatataa gaatgacgaa aaggagagaa aatggttatt agtattcgcc
     8461 gctcacggca tgaggaaggg gaggaactcg ttgcgatttg gtgtcgatct gtcgatgcca
     8521 cccacgattt tttatcagca gagtatcggg ccgagctgga agagctggtg cgttcctttc
     8581 tgccggaagc accgttgtgg gtcgcggtta atgaacggga gcagccggtt gggtttatgt
     8641 tgttaagtgg gcagcatatg gatgcgctgt ttatcgatcc tgatgtgtgc ggctgcggcg
     8701 tgggtcggat gctggtggag catgcgctct cgatggcacc ggaactgaca accaacgtta
     8761 atgagcaaaa tgagcaggcg gttgggttct ataagaaggt gggttttaag gttacgggac
     8821 gctctgaggt ggacgatttg gggaaaccgt atccgttgct gaatctggcg tatgtggggg
     8881 aataagccgc ctctgccgtg cctgcccaag ctggagaaga cg
//
LOCUS       DAZOYF010000162         8778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZOYF010000162 DAZOYF010000000
VERSION     DAZOYF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.163"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            82..390
                     /locus_tag="ACHUOF_003468"
                     /pseudo
     CDS             82..390
                     /locus_tag="ACHUOF_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            362..2289
                     /locus_tag="ACHUOF_003469"
                     /pseudo
     CDS             362..2289
                     /locus_tag="ACHUOF_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2273..2476
                     /locus_tag="ACHUOF_003470"
     CDS             2273..2476
                     /locus_tag="ACHUOF_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFR0612507.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2473..4066
                     /locus_tag="ACHUOF_003471"
                     /pseudo
     CDS             2473..4066
                     /locus_tag="ACHUOF_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            4056..5561
                     /locus_tag="ACHUOF_003472"
     CDS             4056..5561
                     /locus_tag="ACHUOF_003472"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR0612508.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATDAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5598..5945
                     /locus_tag="ACHUOF_003473"
     CDS             5598..5945
                     /locus_tag="ACHUOF_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR0612509.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            6003..7031
                     /locus_tag="ACHUOF_003474"
     CDS             6003..7031
                     /locus_tag="ACHUOF_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR0612510.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7083..7469
                     /gene="gpFI"
                     /locus_tag="ACHUOF_003475"
     CDS             7083..7469
                     /gene="gpFI"
                     /locus_tag="ACHUOF_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR0612511.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7481..7858
                     /locus_tag="ACHUOF_003476"
     CDS             7481..7858
                     /locus_tag="ACHUOF_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR0612512.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7845..8429
                     /locus_tag="ACHUOF_003477"
     CDS             7845..8429
                     /locus_tag="ACHUOF_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0612513.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8426..>8778
                     /locus_tag="ACHUOF_003478"
     CDS             8426..>8778
                     /locus_tag="ACHUOF_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR0612514.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMA"
BASE COUNT         2050 a         2070 c         2690 g         1968 t
ORIGIN      
        1 gcagccttca gggaaaaata tcatcagatg gctgcttaaa aaaagaacaa atggtagtgt
       61 ccgctattgc cagtacacct caaactactc attgccagat gggaactgac cgcagaacag
      121 gctgtcgcac agcagttaaa aaatcaggtg tcaaaaggaa atcttatcga taccggattt
      181 tgtatttttg cccttagtaa gctggcgatg gctttatcca gtacgcttga ctccattcct
      241 ttgtccatgc aacgacagtt cccggattta acgccgcgtc atattgatca tctgaaaatc
      301 cttattgcta agggagcgaa tcagtgcgcg cgtgcggggg ataaattacc ggatttactc
      361 gatgaatata tcagaacaac aactgagtaa tatgatggtg gcagtcacta tcgcactgca
      421 gccactggtc agggtattgc cggtgacggc tgtagaatgg gccgatcaaa attattacct
      481 gcctaaagaa tcgtcatacg gtgaggggga atggaaaacg ctgcagtttc aggtcgccat
      541 catgaactgc atgggcaatg acgagattcg tacagttaac ctgattaaat ctgcccgtgt
      601 tggttataca aaaatggtgc ttggtgtgat cgggtatttt attgagcaca aatcccgcaa
      661 cactcttctt tttcagccga cggactcagc tgctgaagat tttatgaagt ctcacgtgga
      721 agcaacaatt cgtgatgttc cttgcctgaa aaaactttct ccctggctgg gccggaaaca
      781 ccgggataat acgctcacac tgaagcgttt ttcctctggt gtggggttct ggtgtttggg
      841 tggtgctgct gccaaaaact accgtgaaaa atctgtcgat gtggtctgct atgacgaact
      901 ctcctcgttt gaaccggatg tggaaaaaga aggttcgcca acactgcttg gcgataaacg
      961 tattgaaagt tcggtatggc caaaatccat acgcggttca acgccaaaaa ttaaaggctc
     1021 ctgtcagatt gaaaaagctg cgaatgaatc tgcgcatttc atgcggtttt atgtcccttg
     1081 ccctcattgc ggggaggagc agtatctgaa gtttggcgat gatgcgacgt cgtttggcct
     1141 gaaatgggag aagggtaaac cggaaacggt gtattacctg tgtgaacata atggctgcgt
     1201 gatccgtcag tctgaacttg atcagaccgg ggggcgctgg atttgtgaca ataccgggat
     1261 gtggacgtgt gacggtctga cgtttttcag tgcttcgggt aatgaaattc cgccgccgcg
     1321 ctccatcact ttccatatat ggacggcgta cagtccgttc accaccaggg tacagatagt
     1381 ctgtgactgg ctggatgcac tgaaagatcc caacggcgtg aaaacttttg tgaacaccac
     1441 gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg tactgatgga
     1501 taaggttgtg ccttacacgg caacggtgcc tgtccgggtg gtttatctga cggcgggcat
     1561 tgactcgcag cgaaaccgtt ttgagatgta tgtctggggg tgggctccgg gagaggaagc
     1621 ctttctggtg gataaaatca tcatcatggg acgtcctgat gaggaagaga cgctgttacg
     1681 tgtggatgcg gcgatcaata aaaaataccg ccatgcagac ggtaccgaaa tgaccatttc
     1741 ccgtgtctgc tgggatatcg gggggatcga tggcgaaatc gtgtatcaga ggtcaaaaaa
     1801 acacggtatt ttccgggtac tgccggtaaa aggtgcctct gtctatggca agccggtgat
     1861 caccatgccg aaaacccgca atcagcgggg tgtgtatctg tgtgaggtgg ggacggatac
     1921 cgcaaaagaa attctctatg cccgaatgaa ggccgatccc acccctgtga atgaggccac
     1981 atcgtatgcc atccgctttc ctgataatcc ggagattttt tcgcagacag aggcacaaca
     2041 actggtggcg gaggaactgg tggaaaagtg ggaaaaagga aagatgcgtc tgttgtggga
     2101 taacaaaaag cggcgtaacg aagcgctgga ctgcctggtg tatgcctatg cggcattacg
     2161 tgtgtccgtg caacgctggc aacttgatct ggctgtgcag ttgcatcccg caaggatgca
     2221 actgcacaac cgacccttga agaactggca gcgaagctgt ccggaggagt gaatggttac
     2281 agtcgctgaa ctgcaggcgc tgcgtcaggc gcgccttgat ttattaaccg gtaaacgggt
     2341 tgtgtcggta cagaaagacg gtcgcaggat cgaatatacg gcagcgtccc tgaatgagct
     2401 taaccgtgcg atcaacgatg cggagtcggt tctgggtacg acccgacgcc gccgaccgct
     2461 gggagtgagg ttatgaaacg aacgcctgtc ctgattgatg tgaacggcgt tccgcttcgt
     2521 gagagtctca gctacaacgg aggcggcgca ggatttggcg ggcaaatggc agagtggtta
     2581 ccaccggcgc agagtgccga tgcggccctg ttgcctgcat tgcgtctggg gaatgcccgg
     2641 gcagatgatc tggtgcgcaa taacggtatc gcggctaatg cggtggccct gcataaggat
     2701 catatcgtcg ggcacatgtt tcttatcagc taccgtctga actggcgttg gctggggatg
     2761 cgggagacca cggcaaaaag ttttgtcggg gaggtggaag cagcctggtc ggaatacgca
     2821 gaaggaatgt ttggcgagat cgacgtggaa gggaaacgca cgtttacgga atttatccgt
     2881 gaaggggtgg gcgtgcatgc ctttaacggc gaaatctttg tgcagccggt ctgggatacg
     2941 gaaaccacgc agttattccg tacgcgtttt aaagccgtga gtccgaaacg ggtggacacg
     3001 ccaggacacg gtatggggaa ccactttctg cgggccgggg ttgaggtcga tcggtatggg
     3061 cgggcgattg cgtaccatat ctgtgaggat gattttccgt tctctggtgc cggacgctgg
     3121 gagcggatcc cgcgtgagct tcccaccggg cgtccggcca tgctgcatat ttttgagccg
     3181 gtggaggacg ggcagacccg tggggctaat cagttttaca gcgtaatgga gcggatgaag
     3241 atgctggact ccctgcaggc cacgcagctt cagtcggcca ttgtgaaggc gatgtatgca
     3301 gccacaatcg aaagtgaact tgataccgaa aaggcatttg aatatatcgc gggggcaccg
     3361 caaggtcagc aggataatcc gcttattaat attctggaga agttcacccg ctggtatgac
     3421 acgaataacg tgacgctggg cggtgttaaa attccgcacc ttttttcctg gcgatgcgct
     3481 gaacctgcag acagcgcagg attcagacaa tggattttcg gcgcttgaac aggcgctgct
     3541 gcggtatatt gcagccggtc ttggcgtttc ctacgaacag ttgtcccgtg attactcgaa
     3601 ggtcagttac tcaagtgccc gcgcctccgc caatgagtcg tggcgctatt ttatggggcg
     3661 gcgaaaattt attgcggccc ggctggccac gcagatgttt tcctgctggc tggaagaggc
     3721 gcttcttcgg gggattattc gtccgccacg ggcgcgtttt gatttttacc aggcgcgttc
     3781 agcctggtca cgggcagagt ggattagctc cggaagaatg gccattgacg ggctcaagga
     3841 ggtccaggaa tcggtgatgc gcattgaggc cggactgagc acgtatgaga aagaactggc
     3901 actgatgggc gaagattatc aggacatttt ccgccagcag gtcagggaat ccgcagagcg
     3961 cgaaaaagcc ggactctcac gtccggtgtg gatagcccag gcgtatcagc agcagatagc
     4021 ggagagtcgc aggccggaag aggagacaac atcacgtgag acgtaatctt tcacatatta
     4081 tcgctgcggc attcaatgag ccgctgcttc tggagcccgc ctatgcgcgg gttttctttt
     4141 gcgcgctcgg gcgtgagatg ggcgcagcaa gcctttcggt accgcaacag caggtacagc
     4201 ttgacgctcc cgggatgctg gctgaaacgg acgagtacat ggccggaggc aaacgacctg
     4261 cccgtgttta ccgggtggtg aacggtattg ctgtactgcc ggtgaccggc acgctggtac
     4321 accggctggg tggcatgcgg ccattttccg gaatgactgg ctatgacggc attgtcgcct
     4381 gtcttcagca ggcaatggcg gacactcagg tacggggcgt actgctggac attgacagtt
     4441 ctggcgggca ggccaccggc gcgtttgact gcgctgacat gatttaccgt ctccggcagc
     4501 tgaagcctgt ctgggcactg tgcaatgaca cggcctgttc agccgccatg ttactggcat
     4561 cggcctgctc ccgacggctg gttacccaga catcccgtat cggctccatt ggggtgatga
     4621 tgggccatgt cagctatgcc ggtcatctgg cacaggccgg tgtggatatc acgctgattt
     4681 acgccggatc gcacaaggtg gatggcaatc agtttgaatc cctgccagca aaggttcgcc
     4741 aggacatgca gcagcgcatt gatgcggcgc gccggatgtt tgctgaaaaa gtggcgatgt
     4801 ataccggttt atctgtggat gcggttatgg gaacagaggc cgccgttttt gaaggtcagt
     4861 ccggtattga ggccgaactg gcggatgaat taatcaatgc gtcggacgcc atcagcgtga
     4921 tggctgcgac gctgaacaca cacgatacag gaggcactat gccgcaatta actgcaacgg
     4981 atgctgccgc gcaggagaac cagcgagtga tggggatcct gacgtgtcag gaagcgaaag
     5041 gacgtgaaca actggccacg atgctggcag ggcaacaggg tatgagcgtt gagcaggccc
     5101 gggcgattct ggccgtggcg gcaccgcaac aatctggtgt atctgcgcag agtgaagccg
     5161 atcgcattat ggcatgtgaa gaggcgaaag gacgtgaaca actggcggca acgctggcgg
     5221 cgatgccgga gatgacggtg gaaaaagccc gtccgattct ggctgcttca ccgcaggcga
     5281 atgccggacc ctcacttcgt gatcagatta tggccctgga tgaggcaaaa ggggcagagg
     5341 cgcaggctga aaaactggcg gcctgtccgg ggatgaccgt ggagaatgcc cgggctgtac
     5401 tggcggcagc ctcaggcagg gcagaaccgg tttctgcatc cacaaccgcc atgtttgaac
     5461 atttcatggc gaaccattca ccggcagcgg tacagggtgg cgtgccacag acgtcagcag
     5521 acggtgatgc ggacgtgaaa atgctcatgg ccatgccatg aagtcagtgc tgaccatcaa
     5581 tatgaggttt ttacaatatg gtaacgaaaa ccatcactga acagcgtgcg gaagtacgta
     5641 tttttgccgg taatgatccg gcccataccg ccacaggctg cagcgggatt tcctcggcaa
     5701 caccggctct gacgcccctg atgctggatg aggataccgg gaaactggtg gtctgggacg
     5761 gacagaaggc aggccatgtg gttggcatac tggtactgcc gcttgaaggt acagagacgg
     5821 tgctgacgta ttacaagtcg gggacctttg cgacggaggc catccactgg cctgagagag
     5881 tggatacaca caaaaaggcg aatgcctttg ccggcagtgc cctgagtcac gcagcgctgc
     5941 cgtaacattt tatcaggtca ctgcggtagc ctgactgatt tctgaatgaa aggaactgat
     6001 ttatgggatt gtttacgacc cgccagttac tcggttatac cgaacaaaaa gttaaatttc
     6061 gtgcgctgtt tctggagctg tttttccgcc gtacggtgaa tttccatacc gaagaggtaa
     6121 tgctggacaa aattaccgga aaaacgccgg tggcggccta tgtctccccg gttgttgaag
     6181 gaaaggtgtt gcgtcaccgt ggcggtgaaa ctcgtgtact gcgcccgggc tacgttaagc
     6241 cgaaacacga atttaattac cagcaggcgg tggagcgtct tcccggtgaa gatccggccc
     6301 agctgaacgg cccggcttac cgtcgcctgc gtatcatcac cgataacctc aaacaggaag
     6361 agcacgccat tgtccaggtg gaagagatgc aggcggtgaa tgccgtgctg tatggcaaat
     6421 acaccatgga aggagagcag tttgaaactg ttgaggtcga ttttggccgg tcggtgggaa
     6481 ataacattac gcaggcagga gggacggagt ggagcgctca ggaccgcgat acctttgatc
     6541 cgacgcatga tctggatgcg tactgtgatt ttgcttccgg caccatcaat attgccatta
     6601 tggatggtaa ggtctggcgt ctgctgaatg gctttaagct gttccgcgaa aaactggata
     6661 cccgtcgtgg ctcaaattca cagctggaaa cggcagtgaa agacctgggg gcggtggtgt
     6721 ccttcaaagg gtattatggt gatctggcaa tcgtggtggc aaaaacgtcc tatgtggcag
     6781 aggacggtac cgaaaaacgt tatctgccgg acggcacact ggtactggga aatacggccg
     6841 cagagggtat tcgttgctac ggtgccattc aggatgcgca ggcattgtct gaaggcgtgg
     6901 tggcgtcttc ccgttatccg aaacactggc tgaccgtggg tgatccggcg cgtgaattca
     6961 ccatgacgca gtccgcgccg ctgatggtgc tgccggatcc ggatgagttt gtggtggtgc
     7021 aggtgaaata atccgtgagc gggagctaaa tgctcccgtt tttcattcaa tcaggagcgg
     7081 gtatggctac gaaagaagaa aacctgaaac gtctttgtga gctggctgag aggctggggc
     7141 gtgaaccgga tgtctccggt agtgcggcag atattgcgca gcgagtggcg gaactggagg
     7201 aagagcttgg agatgcgggg gaggtcgcag agccggatac gtactcacca tcgaaagatg
     7261 atccgaccgc gcataaaggc gagtcagttc ctgaacagcc aggcattgtt accggagacg
     7321 aaacggggca ggttacggtg gtggcactgg cgacactgca tacaaaatgt aatggttcag
     7381 tgatatttgt ttcaccggga acgtcgtttc gtgtttctgc tgaggtggct gccagcatgg
     7441 cggcaggtgg tctggcaaaa aggcagtaac aggagatgtc gtggacggtt tcgataatct
     7501 gtttgatgct gcgcttgccg gtgtcaatga agtcattctc cgtgatatgg ggatcagcgc
     7561 cgtcattacg tccggagaac ttgaggggac acacctgaca ggggtttttg atgatcccga
     7621 aagtatttct tttgttgccg gggggatccg gcttgaggat tcctcaccgt gcctgtttgt
     7681 gaaaacggca gatatatcgc agttacgccg tcaggatacg ttaactatcg gggatgattc
     7741 tttctttgtg gatcgtatta ctccggatga cgggggatgc tgttatatcc ggttaaggcg
     7801 gggcagtcct gccccacaga ataatgccag aatgaggcga tatgatgaag ggacttgaga
     7861 atgccatccg caatctgaac agcctcgata cccgcatggt gccacagacc agcgcatggg
     7921 cggtaaaccg tgtggcggca aagattgttt ctgtcgccac acggcaggtt gcgcagaata
     7981 ccgttgccgg ggataaccag gtgaaaggta ttcccctgaa aacggtcagg gagcgtgtca
     8041 ggctgcttaa agccagcccc tcaggaaaaa tgtatgcccg catgcgtgtt aaccggggta
     8101 acctgcccgc cattaagctg ggtaccgcac aggtcagact ggcccgctcc cggcatggca
     8161 gcaactcacg tcatcgtggc agcgtactga aagtggggaa ataccttttc cgggatgcgt
     8221 ttattcagca actggcgaat ggtcgctggc atgtgatgcg gcgtattgac ggcaaaaatc
     8281 gttaccccat tgatgtggtg aaaatcccga tgtccggacc gctgacacag gcatttgaag
     8341 atgcccgcga ccgcatcatt gctgcggaaa tgccgaaaca gctggggtat gccctgaaac
     8401 aacaactgag gttacatctg agtaaatgaa ccgacacaca caaatccgtc agagcgtgct
     8461 ggcacgcctt cgggaacagt gtggagacag cgccacgttt tttgacgggc ttccggcatt
     8521 tattgatgcg caggaactgc ccgccgtggc ggtgtggctg agtgatgcgc agtacaccgg
     8581 aaaaatgacg gatgaggacg actggcaggc tgttctgcat attgccgttt tcgtccgtgc
     8641 acaggcgccg gattctgagc tggatacgtg gatggagaac accatatttc cggccatgaa
     8701 ggatattccg gtgctttccg gactcatcga caccatgatc ccactcggtt ttaactatca
     8761 acgtgataat gagatggc
//
LOCUS       DAZOYF010000163         8760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZOYF010000163 DAZOYF010000000
VERSION     DAZOYF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.164"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..439
                     /locus_tag="ACHUOF_003479"
     CDS             <1..439
                     /locus_tag="ACHUOF_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR0612515.1"
                     /translation="QKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQ
                     FAVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYA
                     KAKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACHUOF_003480"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACHUOF_003480"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR0612516.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACHUOF_003481"
     CDS             complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACHUOF_003481"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR0612517.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACHUOF_003482"
     CDS             complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACHUOF_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR0612518.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4441..5148)
                     /locus_tag="ACHUOF_003483"
     CDS             complement(4441..5148)
                     /locus_tag="ACHUOF_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFR0612519.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPGFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACHUOF_003484"
     CDS             complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACHUOF_003484"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR0612520.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6006..6209)
                     /locus_tag="ACHUOF_003485"
     CDS             complement(6006..6209)
                     /locus_tag="ACHUOF_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0612521.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6368..7876
                     /locus_tag="ACHUOF_003486"
                     /pseudo
     CDS             6368..7876
                     /locus_tag="ACHUOF_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7873..>8760
                     /locus_tag="ACHUOF_003487"
     CDS             7873..>8760
                     /locus_tag="ACHUOF_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR0612522.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEP"
BASE COUNT         2032 a         2389 c         2251 g         2088 t
ORIGIN      
        1 gcagaaggtg gcgttaaatc tcgcttcagg tgaagggaat ttctttatcc gtcctggcgg
       61 cgtgttttat gtcgcgggag ataaagtcgg catcgttcgt ctggatgctt ttaaagccag
      121 taaagatatt cagtttgcgg tgcagtcagg accaatgttg atggaaaacg gtgtaattaa
      181 tccgcgtatt catcccaacg tcgcctcacg taaaattcgt aacggtgttg ggattaataa
      241 acatgggaat gccgtgtttt tgttgagcca gcaggcgaca aatttttatg attttgcctg
      301 ttatgccaaa gcgaaactga acgttgagca gctgctttat cttggtggca cgatttcaca
      361 tatgtatatg aaaggcgggg caatcccgtg gcaacgctat ccttttgtca ccatgatttc
      421 cgtcgagcgg aaaggttaga atgatagctt tgtgtcagat actaaatatg ctgctgatgc
      481 cggatgcggc gtaaacgcct tatccggcct acatgacgtt gcaatttatt gaatctgaat
      541 gattttttag gccaaataag gtgcgcagca ccgcatccgg caacgttaca ccgactccag
      601 ccgggcgtaa gctgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg
      661 gctatgttca ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg
      721 tacacgctgg ccgagcttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg
      781 atggctgacc gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg
      841 caactcgcca ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt
      901 ttccgcgtag gtcagcgtca gtttctgcat cgcgcgggtt acgccaacgt aggccagacg
      961 gcgttcttct tccagacgcc cgccttcatc cagcgacatc tggcttggga acatgccctc
     1021 ttccataccg acgataaaca cctgcgggaa ctccagacct ttcgccgagt gcatggtcat
     1081 cagttgtacc gcatcctgcc aggtatccgc ctgcccttcg cctgcttcca gcgccgcatg
     1141 ggataggaac gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg
     1201 cgttgccgtc accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc
     1261 ctgctcgtac atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag
     1321 cggcatatcg gcagtttcct gcgctaaggc gtcgatcagt tccataaacc gctgtaaggc
     1381 gctggcagca cgtccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag
     1441 tgttaactgg cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc
     1501 ccgcgttggc gtattcacca cacgctcaaa ggccgcgtcg tcgttacggt tggcaatcag
     1561 gcgcagatac gagagcgcat ctttgatttc ctggcgttcg aagaagcgca tcccgccgta
     1621 aatacggtac ggcatactcg cctgcaacag tgcttcttcc agcacacgcg actgggcgtt
     1681 actgcggtag agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat
     1741 gcggttaacc acaaaacgcg cttcatcgag ttcgttaaaa gcgcaataga gggaaatagg
     1801 ctcaccgtcc gcgccatcgg tccacagttt tttacccaga cgcccgttat tgttttcaat
     1861 cagggcgtta gcggcgctca gaatattgct ggtagagcgg tagttttgct ccagacgaat
     1921 aatttcggca ccggggaaat cattaaggaa acgctgaata ttctccacct gcgccccgcg
     1981 ccagccgtag attgactggt cgtcatcacc gacgatcatc actttgccgg tgtcgcccgc
     2041 cagcaggcgg atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat
     2101 attggtaaaa cgttcgcggt agtgttgtag gatatgcggc ttgttaagcc acagttcgtg
     2161 agcgcgcagc agcagctcgg cgaagtccac caggcccgcg cgatcgcacg cttcctgata
     2221 cgcctgatac accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg
     2281 cggacgcagg ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca
     2341 ctgcttctcg tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc
     2401 gctatcgagg atctggaaat cctgcggcag attggcgtcc atatggtgcg cacgcagcag
     2461 gcggtgcgcc agcccgtgga aggtgcctac ccacatgccg ccctggctgg tgcccatcag
     2521 ttgtccgata cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat
     2581 cgagtatggc gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg
     2641 cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc
     2701 ttcgcgttgt ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc
     2761 gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac
     2821 agatttgctg attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgtggc
     2881 agtaaacggc tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc
     2941 tgcattccgc tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag
     3001 atctcgccga tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt
     3061 tttgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac
     3121 agttccggct gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt
     3181 tttaaggtgt cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta
     3241 atcattgctg cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc
     3301 gcttgttcaa tcgaacgaaa acgccaacgt gtcacgtcgt gataaatctc gggttccgct
     3361 tcccgtaccg cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga
     3421 tgataatttt gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta
     3481 tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa
     3541 aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt
     3601 gttgaaaatc aagatgagta tagatttgcg tggtggagag gttggcatga cccagtagct
     3661 cctgcacacc acgaagatcg ccgctcgact ccagcatatg cgtagcgaac gagtgacgta
     3721 atttatgtgg gtgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt
     3781 tctgcacatt acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt
     3841 cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac
     3901 caatcggcag gcggcgctct ttgctgcctt tccccatcac ccacacttcg ccggactcca
     3961 ggtcgaggtg tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca
     4021 tcacttccag cattgcacgg tcgcgtacag cgaggggatc gttgatatca atatccagca
     4081 gccgattcat atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg
     4141 aaactccttt cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc
     4201 tacgtagcgc agaaagccgt aacgccagac ttgctgctcc cagcccttta cggcgactgc
     4261 gtacagcaaa attgcgtacc atcgctgcat cgcattgttg ccagctttgc aggccgtttt
     4321 cgctggcaaa attgatgatc gcctcaagct gacgctggta gttaagtagg gttatcgggc
     4381 taagctgacg ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg
     4441 tcatacgcgt tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag
     4501 taactgcgtt ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacaac
     4561 acccaaatca gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc
     4621 cggtagcacc accagcagct ccggtccgtt aagcggccca agatagtgct gttcctgccc
     4681 caaacgctga atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt
     4741 cgacggcgca cctaagcgcc agcgatctgg aaacaggcgc agactcgcac cagctaggcc
     4801 gagatcgcgc gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt
     4861 gagactgcgc tgcaggtaga gtaggcgata aaacagccct tcgttggcga tagcctgctc
     4921 catcaacaac gccatgttct cttccagaac atgaatgtga ttacgtgcgc gggccatgtg
     4981 ccactcgacc aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc
     5041 gcgcgcattg cggataaaaa acccaggatt tttaatcaga taatcgacaa ccgcccggtc
     5101 atcaagctcc gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat
     5161 ccatcgtaga catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag
     5221 gcgatatcaa gacgaccgcc ggggagttcc acgcgtactt cttcgtccag caaaccttgc
     5281 tgaatcccta ccgccaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc
     5341 ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata
     5401 ttggcgcgct ccggaaaacg ctcgtgactt tccagaacag gaccaagcgt ttctaccgcc
     5461 gcggtatcga catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg
     5521 cataagattg tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga
     5581 aacggcacgg cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca
     5641 tcggtgacgg tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg
     5701 gtcagtcctt tcagacgcac aaaacgggca aagcagcgcg caccgttacc gcactgcgcc
     5761 acttcactgc catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc
     5821 ggctcaacca ccagcagctg gtcaaaccct acccccaggt gccgatccgc caggcgacga
     5881 atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg
     5941 ccaaggccat gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact
     6001 gacttttagt aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc
     6061 accgtggatt gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca
     6121 ggcgggaaat agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat
     6181 acagtgagtg ccttaaacac gtttttcatt atggattgcc tgtaaagttc atcgctttct
     6241 gctttctatc atcgcaggag aaggcgtaaa agcaagcgtt tagcgactct ttatgaagtc
     6301 tgaaaagtga ggaaatgctg cgagtataac gcttactcgt gaggatttat gttctgctgg
     6361 cagtttcgtg atcaacacct cgcgccagga tggctttatg cactatcgca atgtggtttg
     6421 tttgttgtct tgttcattgt ttttatcgtc ggcatgggga tgtcggcttt ccgcacctga
     6481 acataatatt taccagaaac agggaaaagg cgtggtgtac ctgcgccctt ataaagaaac
     6541 aaatctttct ctacctgacg tgaattacaa gagtttgcgt cggttaccca atttattaat
     6601 agacccgaca acactagacg aatgggataa agaaccgcct ctgactgatt tgacaaccga
     6661 ctacctctat gagggcgcac aggcctggta tcctcattat tcatggcata gcgatggtcg
     6721 caatatcctc tacgcgggtg aagttgtgca aaaccctccc ggcacacctc ccgtcgatgt
     6781 cgcctcgttc aaggcatggg gtgattttgc cgcagataaa cacagtctct attttgaagg
     6841 caagcgcacc gatgacaatg gtggtggaaa tagcttggat atcaaaacgc tgcatcaggt
     6901 ggaattccgg cctccctggg atcctgactt actggggctg atattacgtg acgctaattt
     6961 cttgtatatc aatgggcatc gcctcgctga ccccgagagt ttccgtgtgc tggcgcagaa
     7021 gtcctgggat cagcgaggaa agttttccac cacgtttaac ccctgcattg ccgtaccatt
     7081 tggcccctgg gataccctgg ctcgtacacg gaccaaaatc ctgctcaacg gcgaacagct
     7141 tgatgccgac ccggacacct tttccgtcgt gcgctggatg cccggctcac tcttgacctg
     7201 gcgtgataaa aacgggctgc agcgtaaagt cctcgacaag gaaaatctgg cgtgggatga
     7261 agatttaaca aagcactgtc tggatttttc tctgctggaa aagaaagtgt tctggcgtaa
     7321 aggacctgct tgtaaacagg aagaattacc cggactcgat ccggagcagt ttcaacccat
     7381 cagtgatgct gtcgcccagc atcaggactc gctttatacc atcatcgaaa cagagtctgg
     7441 taaccgtaag ctggagatcg tgaaacttga tgatcccaat cttattatca acaaacgttt
     7501 caacgccggg aaacgccacg gctatttact tacgcgtgcc gaagggtagc cataccattc
     7561 cggtttacac gtgtttgaat ctgacggacc gctgatctta ctggataacc gttctccgga
     7621 tgaacgcgaa gcccatctta atgaccatcc ctttttgcgc agatggtatg cccgcgataa
     7681 ccgctacatt tacagctttg atggcgcgca gctctggcga taccgcaccg ctgatccgaa
     7741 acaagttcgc ttaatctgga aggaacaaca ttcgggatat gtctatggcg taaattacaa
     7801 aacgggatat ctggacggaa aaattactga tgacggcgaa tttattcctg ccccgcgcaa
     7861 tgaggcgaca aaatgaaggg tcaaataatc acagttgcac tcatcctgtt gggacacaca
     7921 tttctgacgc ctgctgtaca ggccataggg ttcgactatt acaatgatca cggtgttatg
     7981 tcttacggta aaggttattg gggggataag aaaatcattg cccagtttcc ggagatgagc
     8041 agggccggtt tgcgccttgt gaaaaatatc tctgggaaaa cgggttatat accttccgat
     8101 gaaataagca tagaaaatga ggaataccac tgggtgacag acggccgcgt tattctctgg
     8161 cgtggcaaaa tcgttagcaa tccaccggga acactcactg tcgatattgc cagctttcag
     8221 gctatgggcc gcttcgcggt cgataaatat agcctctatt ttgacggaca gcgcaccgaa
     8281 agtaatagcg gcgcgtcacg tgttgagctg gcgacactaa aagctatcga aggtaactct
     8341 accacgctgg tggatagtaa aaatctctac ttgtccggtc gacgtcaggg gagcagcagt
     8401 gatgttactg tattagaaaa aagatggtgg ggtattaatc cacgtcttat gagtgtaaac
     8461 agaaattcat attccaatga tctgcttatc cgcagtgggc agaatattta tttaaatggc
     8521 gttcacctta cggcgaatgc agactcattt gagataattc gctggatacc tcattcactg
     8581 ctggtttttc gcgacaataa gggtctgcat cgttatccct ttggtcaatt atcaggcaaa
     8641 gcgatacccg tagatgatga cgtctctttt gaagtagggg aaagtcgcgt tcgctggcgt
     8701 aaacagctca cgcccgaccg tcagtggagc aagtggatag acctaccagg tattgaacct
//
LOCUS       DAZOYF010000164         8756 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZOYF010000164 DAZOYF010000000
VERSION     DAZOYF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.165"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(435..641)
                     /locus_tag="ACHUOF_003488"
     CDS             complement(435..641)
                     /locus_tag="ACHUOF_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR0612523.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="ACHUOF_003489"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="ACHUOF_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR0612524.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="ACHUOF_003490"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="ACHUOF_003490"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR0612525.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4489
                     /gene="kdpB"
                     /locus_tag="ACHUOF_003491"
                     /pseudo
     CDS             2739..4489
                     /gene="kdpB"
                     /locus_tag="ACHUOF_003491"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="ACHUOF_003492"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="ACHUOF_003492"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR0612526.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="ACHUOF_003493"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="ACHUOF_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8722
                     /gene="kdpE"
                     /locus_tag="ACHUOF_003494"
                     /pseudo
     CDS             8037..8722
                     /gene="kdpE"
                     /locus_tag="ACHUOF_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
BASE COUNT         1882 a         2246 c         2506 g         2122 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgttaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     8461 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     8521 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     8581 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     8641 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     8701 tgggtatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgatt
//
LOCUS       DAZOYF010000165         8694 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZOYF010000165 DAZOYF010000000
VERSION     DAZOYF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.166"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            63..305
                     /locus_tag="ACHUOF_003495"
     CDS             63..305
                     /locus_tag="ACHUOF_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFR0612527.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(337..987)
                     /gene="tetR(A)"
                     /locus_tag="ACHUOF_003496"
     CDS             complement(337..987)
                     /gene="tetR(A)"
                     /locus_tag="ACHUOF_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFR0612528.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1093..2292
                     /gene="tet(A)"
                     /locus_tag="ACHUOF_003497"
     CDS             1093..2292
                     /gene="tet(A)"
                     /locus_tag="ACHUOF_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFR0612529.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2324..2788)
                     /locus_tag="ACHUOF_003498"
                     /pseudo
     CDS             complement(2324..2788)
                     /locus_tag="ACHUOF_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            complement(2821..5826)
                     /locus_tag="ACHUOF_003499"
     CDS             complement(2821..5826)
                     /locus_tag="ACHUOF_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="HFR0612530.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSR
                     GDHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTR
                     REHAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILP
                     GATTLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGP
                     VTISGPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQK
                     RMAVLVAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALA
                     SACSYLLKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRV
                     RRFLPHLLNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVIN
                     KEKHITRRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRS
                     LGHPTDPQEAIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPD
                     SLKRLSKMISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMA
                     EACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGG
                     GEVASADGMRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDS
                     IFVLEGLLEQETGLNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDH
                     DADYGVLNDIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLT
                     QAIIEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYT
                     DGQEDQLGTLGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINML
                     GHYSFTLAELVTKGHLRPLKEASEAENVA"
     gene            5990..6547
                     /locus_tag="ACHUOF_003500"
     CDS             5990..6547
                     /locus_tag="ACHUOF_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0612531.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            6730..7590
                     /locus_tag="ACHUOF_003501"
     CDS             6730..7590
                     /locus_tag="ACHUOF_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR0612532.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(7738..8163)
                     /locus_tag="ACHUOF_003502"
                     /pseudo
     CDS             complement(7738..8163)
                     /locus_tag="ACHUOF_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            complement(8301..8693)
                     /locus_tag="ACHUOF_003503"
     CDS             complement(8301..8693)
                     /locus_tag="ACHUOF_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFR0612533.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
BASE COUNT         1957 a         2397 c         2385 g         1955 t
ORIGIN      
        1 cttagcgtac gattttttcc gaattctgcg ggctccccta tctcatctgc gcaaggcaga
       61 acgtgaagac ggccgccctg gacctcgccc gcgagcgcca ggcgcacgag gccggcgcgc
      121 ggacccgcgc cacggcccac gagcggacgc cgcagcagga gcgccagaag gccgccagag
      181 aggccgagcg cggccgtgag gcttggacgc tagggcaggg catgaaaaag cccgtagcgg
      241 gctgctacgg gcgtctgacg cggtggaaag ggggagggga tgttgtctac atggctctgc
      301 tgtagtgagt gggttgcgct ccggcagcgg tcctgatcaa tcgtcaccct ttctcggtcc
      361 ttcaacgttc ctgacaacga gcctcctttt cgccaatcca tcgacaatca ccgcgagtcc
      421 ctgctcgaac gctgcgtccg gaccggcttc gtcgaaggcg tctatcgcgg cccgcaacag
      481 cggcgagagc ggagcctgtt caacggtgcc gccgcgctcg ccggcatcgc tgtcgccggc
      541 ctgctcctca agcacggccc caacagtgaa gtagctgatt gtcatcagcg cattgacggc
      601 gtccccggcc gaaaaacccg cctcgcagag gaagcgaagc tgcgcgtcgg ccgtttccat
      661 ctgcggtgcg cccggtcgcg tgccggcatg gatgcgcgcg ccatcgcggt aggcgagcag
      721 cgcctgcctg aagctgcggg cattcccgat cagaaatgag cgccagtcgt cgtcggctct
      781 cggcaccgaa tgcgtatgat tctccgccag catggcttcg gccagtgcgt cgagcagcgc
      841 ccgcttgttc ctgaagtgcc agtaaagcgc cggctgctga acccccaacc gttccgccag
      901 tttgcgtgtc gtcagaccgt ctacgccgac ctcgttcaac aggtccaggg cggcacggat
      961 cactgtattc ggctgcaact ttgtcatgct tgacacttta tcactgataa acataatatg
     1021 tccaccaact tatcagtgat aaagaatccg cgcgttcaat cggaccagcg gaggctggtc
     1081 cggaggccag acgtgaaacc caacagaccc ctgatcgtaa ttctgagcac tgtcgcgctc
     1141 gacgctgtcg gcatcggcct gattatgccg gtgctgccgg gcctcctgcg cgatctggtt
     1201 cactcgaacg acgtcaccgc ccactatggc attctgctgg cgctgtatgc gttgatgcaa
     1261 tttgcctgcg cacctgtgct gggcgcgctg tcggatcgtt tcgggcggcg gccggtcttg
     1321 ctcgtctcgc tggccggcgc tgctgtcgac tacgccatca tggcgacggc gcctttcctt
     1381 tgggttctct atatcgggcg gatcgtggcc ggcatcaccg gggcgactgg ggcggtagcc
     1441 ggcgcttata ttgccgatat cactgatggc gatgagcgcg cgcggcactt cggcttcatg
     1501 agcgcctgtt tcgggttcgg gatggtcgcg ggacctgtgc tcggtgggct gatgggcggt
     1561 ttctcccccc acgctccgtt cttcgccgcg gcagccttga acggcctcaa tttcctgacg
     1621 ggctgtttcc ttttgccgga gtcgcacaaa ggcgaacgcc ggccgttacg ccgggaggct
     1681 ctcaacccgc tcgcttcgtt ccggtgggcc cggggcatga ccgtcgtcgc cgccctgatg
     1741 gcggtcttct tcatcatgca acttgtcgga caggtgccgg ccgcgctttg ggtcattttc
     1801 ggcgaggatc gctttcactg ggacgcgacc acgatcggca tttcgcttgc cgcatttggc
     1861 attctgcatt cactcgccca ggcaatgatc accggccctg tagccgcccg gctcggcgaa
     1921 aggcgggcac tcatgctcgg aatgattgcc gacggcacag gctacatcct gcttgccttc
     1981 gcgacacggg gatggatggc gttcccgatc atggtcctgc ttgcttcggg tggcatcgga
     2041 atgccggcgc tgcaagcaat gttgtccagg caggtggatg aggaacgtca ggggcagctg
     2101 caaggctcac tggcggcgct caccagcctg acctcgatcg tcggacccct cctcttcacg
     2161 gcgatctatg cggcttctat aacaacgtgg aacgggtggg catggattgc aggcgctgcc
     2221 ctctacttgc tctgcctgcc ggcgctgcgt cgcgggcttt ggagcggcgc agggcaacga
     2281 gccgatcgct gatcgtggaa acgataggcc tatgccatgc gggtcaaggc gacttccggc
     2341 aagctatacg cgccctagga gtgcggttgg aacgttggcc cagccagata ctcccgatca
     2401 cgagcaggac gccgatgatt tgaagcgcac tcagcgtctg atccaagaac aaccatccta
     2461 gcaacacggc ggtccccggg ctgagaaagc ccagtaagga aacaactgta ggttcgagtc
     2521 gcgagatccc ccggaaccaa aggaagtagg ttaaacccgc tccgatcagg ccgagccacg
     2581 ccaggccgag aacattggtt cctgtaggca tcgggattgg cggatcaaac actaaagcta
     2641 ctggaacgag cagaagtcct ccggccgcca gttgccaggc ggtaaaggtg agcagaggca
     2701 cgggaggttg ccacttgcgg gtcagcacgg ttccgaacgc catggaaacc gcccccgcca
     2761 ggcccgctgc gacgccgaca ggatctaggg gtctgacgct cagtggaacg aaaactcacg
     2821 ttaagcaacg ttttctgcct ctgacgcctc ttttaatggt ctcagatgtc ctttggtcac
     2881 cagttctgcc agcgtgaagg aataatggcc gagcatattg atatgtccgt ggcaaagcgg
     2941 ggagaggcgt gcgatatctt catcattcag tgtttcaccc tgcgcccgga gatgatccag
     3001 ggctgcctgc atataaatag tgttccataa cacgacggcg ttagtgacca gccccagtgt
     3061 gcccagttga tcttcctgac cgtcggtata tcgttttctt atctcacctt tttgaccgtg
     3121 acagatggct ctggcaacgg catggcgact ttctccccga ttaagctggg tcagaatgcg
     3181 ccggcggtaa tcttcatcat caatataatt aagcagatac agcgttttgt tgatgcgccc
     3241 cacttcaatg attgcctgag tcagtccgga aggacgttca cttttcagca atgaacggac
     3301 cagcactgaa acctgtactt tgcccagctt cagggagcca gcggtccgga tcatttcgtc
     3361 ccactgaagg actatttttc ggggatctga ttgccctctg gcaatatcat tcagcacgcc
     3421 atagtcggca tcatggtcca ttcgccagaa aaccgaagca ccggcatcag ccaggcgtgg
     3481 agaaaactgg tatcccagca gccagaaaag gccaaagaca agttcgctgg cacctgctgt
     3541 atcggtcata atttcggttg gattcagccc ggtctcctgt tccagaagac cttccagcac
     3601 aaagatagag tccctcagcg tccccggtat aacgatgcca tgaaagccgg aatactgatc
     3661 ggacacaaag ttgtaccagg tgatccctct gttattacca aagtatttgc ggttcggtcc
     3721 ggcattgatt gttctgactg gcgtaacaaa gcgcattcca tctgcagatg ccacttctcc
     3781 tccaccccat atctgtgcca gtggcagcgt tgcctgaaaa tcaaccagtc tggcattagc
     3841 gctggtgata gtttcagccc gcagatagtt cgcttttgtc cagttcagcc ggtgtcgggt
     3901 cagtgcagga acatttgatc tgatcagtgg ttccagaccg atattgcagg cttcagccat
     3961 cagcacggcg ctgatgctga cgggcagatc atcaactctg gcactggctt cactagcatg
     4021 gaaaaactca tcagcaaatc cggtatgggc gttaatttcg agcagcaact ccgttaaatc
     4081 caccggaggg agtagatcac tgatcatttt gctcagtcgt ttcagactgt ccggctcatc
     4141 aagactggcg aggggagaaa ttgtcaaccg gggcttcggg ccagaaacat cgagttcgac
     4201 agcctcattt tcgcaaagac gtgcagcaac ctgtctgtaa cgactatcaa gctgatgacc
     4261 cagagatttt attgcttcct gcgggtctgt cgggtgcccc aaagaacgat aaaccttaat
     4321 ccggtttgcc tgccagtcag caccctgtag taatcttgca cgaggatctc cccaccggtt
     4381 actgccggta acgtagacat ccctccgcct cagactatcc tgcagtttac tgagaaagca
     4441 gagcgtgtat cccctgcggg tgatatgttt ttccttgtta atcaccagcc gtttccatga
     4501 ccgactgata atttccgttg gtgcgtcgtc aaaaaactgc cgccgtgagc tgaactcccg
     4561 gctgaggtag tcacaggcat tcagagtggt aaccccggca ggtgcggatg aaaatttaac
     4621 ggtattcagc agatggggca ggaaacgacg aacgcgcccg tactgctcca ccatttcttc
     4681 atgaaaatta tcgtctgagg gccgggcaat ttcacggaca agcgtgatga tttcagccag
     4741 cttttgcctt gggatgtagc tgaacacctc agcacgaatc gattcgtccg gtgtttcttc
     4801 tttcagcagg tacgaacatg cgctggcgag cgccaatgca gatttatcca gatccttcag
     4861 cgagcggagc cgttttttct gcccaatctt tctggcgtca cggatgataa cggccagcat
     4921 ggcgtccaga acgtccaatg catcatccag cgccagcgtt tcccatgcaa ggacaaaggc
     4981 aaccagaacc gccatccttt tctgcggtga catcctggca atattgaaca ccgaagtcat
     5041 accagcataa cgtgcgagat ttttcaggcg cacagccggg agtgtactca ggttttcagc
     5101 atgcaggcca aaatcgttca gagttttcca gcgttcaatt gcttcattaa acgccggacc
     5161 actgatggtc acagggccct ttttcagtga ttccagtaaa gacaggcggc tgcaatcagt
     5221 tggccccagc agcatctcca gctgtgaacg ctgttcggct gacggtatca gtgccagttt
     5281 gttccacagg cgcaacgtcg ccttttccct tacctctgaa atcaaccggg tcagcgtagt
     5341 ggctccgggg agaataatac gatgttgcat aagccaccct gtcgccagat cgaaaagcag
     5401 gccaggacgt tcgttgctta tccagctccg ggtatataaa agacgggtaa ggcgaaatgt
     5461 ccagggccag gcaaattcac gatactgata gtgctgacgt atcagcgctg catgctcacg
     5521 gcgggtattt tccctctgac cgtattctgc aagaacggtg atatcacgaa tcccgagctg
     5581 tctggcggta aaatgccgga cgccggaagg aatatgattc atatcggtga ggaaggtgcc
     5641 cagaaaacgg acacatccaa tttgcagggc aatgcccaga cggttgtgat cacctctgct
     5701 ttttccgata aattccttgt ctgcttcatc aaggtgaaaa tatcgtgcca gctgaagctc
     5761 atccggttca ccggtgaatc tgccatagct ttcagtctgc tcagtggtca gaaagtcaac
     5821 gggcatatcg gcctccctgc ctgacgggca tttagtaaca tttttccaac cgtacgaaat
     5881 gttataaatt atcggacatc gtaaaactgt tacattaata tgtctattaa atcgtaaatt
     5941 tgtaataata gacatgagtt gtccgatatt cgatttaagg tacattttta tgcgactttt
     6001 tggttacgct cgggtctcaa ccagtcagca gtctcttgat cttcaggtca gagcactcaa
     6061 agacgcaggt gtgaaagcaa accgtatatt taccgataag gcatccggca gttcaacaga
     6121 ccgggaaggg ctggatttgc tgaggatgaa ggtggaggaa ggtgatgtca ttctggttaa
     6181 gaagctcgac cgtcttggcc gcgacactgc cgatatgatc caactgataa aggaatttga
     6241 cgctcagggc gtggcagtcc ggttcattga tgacgggatc agtaccgacg gtgatatggg
     6301 gcaaatggtg gtcaccatcc tgtcggctgt ggcacaggct gaacgccgga ggatcctaga
     6361 acgcacgaat gagggccgac aggaagcaaa gctgaaagga atcaaatttg gccgcaggcg
     6421 taccgtggac aggaacgtcg tgctgacgct tcatcagaag ggcactggtg caacggaaat
     6481 tgctcatcag ctcagtattg cccgctccac ggtttataaa attcttgaag acgaaagggc
     6541 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     6601 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6661 tcaaatatgt atccgctcat gagacaataa ccctggtaaa tgcttcaata atattgaaaa
     6721 aggaagagta tgagtattca acattttcgt gtcgccctta ttcccttttt tgcggcattt
     6781 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6841 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6901 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggtgcg
     6961 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7021 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7081 agagaattat gcagtgctgc cataaccatg agtgataaca ctgctgccaa cttacttctg
     7141 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7201 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7261 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7321 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7381 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     7441 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     7501 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7561 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     7621 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7681 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccatc
     7741 tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc aaatagcccc
     7801 caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga acacgaccat
     7861 cagcggctgc acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat
     7921 aaacaacaag ctccagaata gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca
     7981 ccagattccc gttggaatct gtcggacgat catcacgagc aataaacccg ccggcaacgc
     8041 ccgcagcatc gcgaccgtca tcggtgagaa gttcggcagg tattgggtgg tgacaatgta
     8101 ggtgctgccc caaatggcag gtgctatcgc tgtgaacaat aaatcgggcg tgcgtaatga
     8161 catgcgatta caagacctcc gctacggcga tgggtttttg agagttgcag gcgccgcgat
     8221 gaccgcaccc tgacttggac tggccctgcc tcgcaccaat ccacctgcgg ctatggccgc
     8281 gatcatgcga ggcccgaaaa ttacacgacc tgtacacgtc cgccgaaccc ggaacgcaac
     8341 agagcttcgt gggcatcccg atccgggtcg tagcagcagt cctggaccaa acgcacgtcg
     8401 tagtccgcat cactagccca ggcgacgctt gaaagaacaa cgccggtggt gcttatcccg
     8461 gccatgacaa gcgtgcttac gccgcgcgtc cgaaggtcgg cgtcgagcgc cgtgccataa
     8521 aagacgctgg ctcgcggaca tgcatagaaa aggtcgcccc gttcgatcgc aagcccttcg
     8581 acgggcagac cggtgcgaaa tcgtccgctc gggagatacg gtgagatttg gcgattcgtc
     8641 gccggcggcg catgttcata ctcttcgccg agcgaaaagt tggggaacag cacg
//
LOCUS       DAZOYF010000166         8680 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZOYF010000166 DAZOYF010000000
VERSION     DAZOYF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.167"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACHUOF_003504"
     CDS             complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACHUOF_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR0612534.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            696..914
                     /locus_tag="ACHUOF_003505"
     CDS             696..914
                     /locus_tag="ACHUOF_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR0612535.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            922..1863
                     /gene="ribF"
                     /locus_tag="ACHUOF_003506"
     CDS             922..1863
                     /gene="ribF"
                     /locus_tag="ACHUOF_003506"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR0612536.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1906..4722
                     /gene="ileS"
                     /locus_tag="ACHUOF_003507"
     CDS             1906..4722
                     /gene="ileS"
                     /locus_tag="ACHUOF_003507"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR0612537.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4722..5216
                     /gene="lspA"
                     /locus_tag="ACHUOF_003508"
     CDS             4722..5216
                     /gene="lspA"
                     /locus_tag="ACHUOF_003508"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR0612538.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5303..5752
                     /gene="fkpB"
                     /locus_tag="ACHUOF_003509"
     CDS             5303..5752
                     /gene="fkpB"
                     /locus_tag="ACHUOF_003509"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0612539.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5754..6704
                     /gene="ispH"
                     /locus_tag="ACHUOF_003510"
                     /gene_synonym="lytB"
     CDS             5754..6704
                     /gene="ispH"
                     /locus_tag="ACHUOF_003510"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR0612540.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6770..7684
                     /gene="rihC"
                     /locus_tag="ACHUOF_003511"
     CDS             6770..7684
                     /gene="rihC"
                     /locus_tag="ACHUOF_003511"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR0612541.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7851..8672
                     /gene="dapB"
                     /locus_tag="ACHUOF_003512"
     CDS             7851..8672
                     /gene="dapB"
                     /locus_tag="ACHUOF_003512"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR0612542.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
BASE COUNT         1988 a         2160 c         2461 g         2071 t
ORIGIN      
        1 aggtctaatg aggagatatt tatgaatggt taaagtatat aggaatttaa ttcaaaaata
       61 gagtggtctt aagtttaata cctcaatgtg tatcacaata tccatattct ttgtggggga
      121 gtctggagat tgagtagata ttcttgttca gaatgtatca gccgatggtt ctacgattct
      181 taagccacga agagttcaga tagtacaacg gcatgtctct tttgactatc tggcaaccgg
      241 cagtgtgttc tctcacgcat cacaaaagca gcaggcataa aaaaacccgc ttgcgcgggc
      301 tttttcacaa agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg
      361 ttagccttat gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc
      421 acgatcggtt gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata
      481 gctgcgtata ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta
      541 cgagcctttt cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa
      601 ctcccaaatg tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc
      661 ttttgtcaat ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg
      721 atggcgcagg attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg
      781 cagcaggcgg tcagtttctt cccgtgattt gctccatggc aatgaaaagc cacttctttc
      841 tgatttcggt actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat
      901 tttcactgtt ttgagccaga catgaagctg atacgcggca tacataatct cagccaggcc
      961 ccgcaagaag ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc
     1021 gcgctgttac agggcttgca ggaagaaggg cgcaagcgca acttaccggt gatggtgatg
     1081 ctttttgaac ctcaaccact ggaactgttt gctaccgata aagccccggc aagactgacc
     1141 cggctgcggg aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg
     1201 cgtttcgaca ggcgttttgc ggcgttaacc gcgcaaaatt tcatcagcga tcttttggtg
     1261 aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt tccgctttgg cgctggtcgt
     1321 gaaggcgatt tcttgttatt acagaaagct ggcatggaat acggcttcga tatcaccagt
     1381 acgcaaactt tttgcgaagg tggtgtgcgt atcagcagca ccgccgtgcg tcaggcgctg
     1441 gctgatgaca atctggctct ggcagaaagt ttactggggc acccgtttgc catctccggg
     1501 cgtgtagtcc acggtgatga attagggcgc actataggtt tcccgacggc gaatgtaccg
     1561 ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt
     1621 gaaaagccgt tacccggcgt ggcaaacatc ggaacacgcc caacggttgc cggtattcgc
     1681 cagcagctgg aagtgcattt gttagatgtt gcaatggacc tttacggtcg ccatatacaa
     1741 gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa
     1801 gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac aaaaccggct
     1861 taagcctgtt atgtaatcaa accgaaacac ggaaccgaga atctgatgag tgactataaa
     1921 tcaaccctga atttgccgga aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa
     1981 ccgggaatgc tggcgcgttg gactgatgat gatctgtacg gcatcatccg tgcggctaaa
     2041 aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat
     2101 attggtcact cggttaacaa gattctgaaa gacattatcg tgaagtccaa agggctttcc
     2161 ggttatgact cgccgtatgt gcctggctgg gactgccacg gtctgccgat cgagctgaaa
     2221 gtagagcaag aatacggtaa gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag
     2281 tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca aagactttat ccgtctgggc
     2341 gtgctgggcg actggtcgca cccgtatctg actatggact tcaaaactga agccaacatc
     2401 atccgcgcgc tgggcaaaat catcggcaat ggtcacctgc acaaaggcgc gaagccggtg
     2461 cactggtgcg tagactgccg ttctgcactg gcagaagcgg aagttgagta ttacgacaaa
     2521 acttctccgt ccattgacgt cgctttccag gcggtcgatc aggatgcgct gaaagcgaaa
     2581 tttggcgtaa gcaacgttaa cggcccaatc tcgctggtga tctggaccac tacgccgtgg
     2641 actctgcctg cgaaccgcgc aatctctatt gcacctgatt tcgactatgc gctggtgcag
     2701 atcgacggtc aggccgtgat tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc
     2761 ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg cagagcttga gctgctgcgc
     2821 tttacccatc cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca cgttaccctg
     2881 gatgccggta ccggtgccgt tcacaccgcg cctggccacg gcccggacga ctatgtgatc
     2941 ggtcagaaat acggcctgga aaccgctaac ccggttggcc cggacggcac ttatctgccg
     3001 ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg
     3061 ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc
     3121 tgctggcgtc acaaaacgcc gatcatcttc cgcgcgacac cgcagtggtt cgtcagcatg
     3181 gatcagaaag gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca gtggctcccg
     3241 gactggggcc aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc
     3301 cgtcagcgca cctggggcgt accgatgtca ctgttcgtgc acaaagacac ggaagagctg
     3361 catccgcgta ccctcgaact gatggaagaa gtggctaaac gcgttgaagt tgacggcatc
     3421 caggcgtggt gggatctcga tgcgaaagag atcctcggcg acgaagctga ccagtatgtg
     3481 aaagtgccgg atactctcga cgtatggttt gactccggat ctacccactc ttctgttgtt
     3541 gacgtgcgtc cggaatttgc cggtcacgca gcggacatgt atctggaagg ttctgaccag
     3601 caccgtggtt ggttcatgtc ttccctaatg atctccaccg cgatgaaagg caaagcgccg
     3661 tatcgtcagg tactgaccca cggctttacc gtggatggtc agggtcgcaa gatgtctaaa
     3721 tccatcggca acaccgtttc gccgcaggat gtgatgaaca aactgggcgc ggatattctg
     3781 cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg ccgtttctga cgagatcctg
     3841 aaacgtgctg ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac
     3901 ctgaatggtt ttgatccagc aaaagatatg gtgaaaccgg aagagatggt ggtactggat
     3961 cgctgggccg taggttgtgc gaaagcggca caggaagaca tcctcaaggc gtacgaagca
     4021 tacgatttcc acgaagtggt acagcgtctg atgcgcttct gctccgttga gatgggttcc
     4081 ttctacctcg acatcatcaa agaccgtcag tacaccgcca aagcggatag cgtggcgcgt
     4141 cgtagctgcc agactgcgct gtatcacatc gctgaagcgc tggtgcgctg gatggcacca
     4201 atcctctcct tcaccgctga tgaagtgtgg ggctatctgc cgggcgaacg tgaaaaatac
     4261 gtcttcaccg gtgagtggta cgaaggcctg tttggtctgg cagacagtga agcaatgaac
     4321 gatgcgttct gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa
     4381 gcgcgtgccg acaagaaagt gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa
     4441 ccggaactgg cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc
     4501 tccggcgcta ccgttgcaga ctataacgac gcacctgctg atgctcagca gagcgaagta
     4561 ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg
     4621 cactacaccc aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc
     4681 agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt
     4741 caacagggct acgctggctg tggctggtgg tagtcgtgct gattatcgat ctgggcagca
     4801 aatacctgat cctccagaac tttgctctgg gggatacggt cccgctgttc ccgtcgctta
     4861 atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat agcggcggct
     4921 ggcagcgttg gttctttgcc ggtattgcga ttggtattag cgtgctcctg gcagtgatga
     4981 tgtatcgctc gaaggccacg cagaagctaa acaatatcgc ttacgcgctg attattggcg
     5041 gcgcgctggg caacctgttc gaccgcctgt ggcacggctt cgttgtcgat atgatcgact
     5101 tctacgtcgg cgactggcac ttcgccacct tcaaccttgc cgatactgcc atttgtgtcg
     5161 gtgcggcact gatcgtgctg gaagggtttt taccttccaa agcgaaaaaa caataataaa
     5221 tcctgccgga tgcggcctac agattgctac gaaatcgtag gccgcatccg gcaaaaatcc
     5281 tcaaaaataa gagcaaacct gcatgtctga atctgtacag agcaatagcg ccgtcctggt
     5341 gcacttcacg ctaaaactcg acgatggcac caccgccgag tctactcgca acaacggtaa
     5401 accggcgctg ttccgcctgg gcgatgcttc tctttctgaa gggctggagc aacacctgct
     5461 ggggctgaaa gtgggcgaca aaaccacctt ctcgctggag ccagatgcgg cgtttggcgt
     5521 gccgtcaccg gacctgattc agtacttctc ccgccgtgaa tttatggatg caggcgagcc
     5581 agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc ctggtgtgat
     5641 ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg ccgggcagac
     5701 cgttcatttt gatattgaag tgctggaaat cgatccggca ctggaggcgt aacatgcaga
     5761 tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc agcattgttg
     5821 aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg gtgcataacc
     5881 gctacgtggt cgatagcttg cgcgagcgtg gggctatctt tattgagcag attagcgaag
     5941 tgccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg gttcgtaacg
     6001 aagcgaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg accaaagtgc
     6061 atatggaagt tgcccgcgcc agtcgccgtg gcgaagaatc tattctcatc ggccacgccg
     6121 ggcacccgga agtggaaggg acgatggggc agtacagcaa ccccgaaggg ggaatgtatc
     6181 tagtcgaatc gccagacgat gtgtggaaac tgacggtcaa aaacgaagag aagctctcct
     6241 ttatgaccca aaccacgctg tcggtggatg acacgtctga tgtgatcgac gcgctgcgta
     6301 aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc acgactaacc
     6361 gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg gtcggttcga
     6421 aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc aaacacgcgt
     6481 ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt aaatgcgtcg
     6541 gcgtgactgc gggcgcatcg gctccggata ttctggtgca gaatgtggtg gcacgtttgc
     6601 agcagttggg tggtggtgaa gccattccgc tggaaggccg tgaagaaaat attgttttcg
     6661 aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt agcagcctaa
     6721 gttatgcgaa aatgccggtc ttgttaccgg cattttttat gaagaaaaca tgcgtttacc
     6781 tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg ccgcgatttt
     6841 tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct cggttgagaa
     6901 aactacccgc aatgccctgc aactgctgca tttctggaat gcggagattc cgctcgccca
     6961 gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc acggcgaatc
     7021 gggaatggct ggctacgact ttgttgagca caaccgaaag ccactcggga taccggcgtt
     7081 tctggcgatt cgggatgccc tgatgcgtgc accagagccc gttaccctgg tggccatcgg
     7141 cccgttaacc aatattgcgc tgttactttt acaatgcccg gaatgtaaac cgtatattcg
     7201 tcgtctggtg atcatgggcg gttctgctgg tcgcggcaac tgtacaccaa atgccgagtt
     7261 taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggca tcgaaattgt
     7321 catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc tcgctacttt
     7381 accggagttg aatcgcaccg gaaaaatgct ccacgccctg tttagccact accgtagcgg
     7441 cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc tggtgcgccc
     7501 ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg aatttacctc
     7561 aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg tacaggtggc
     7621 attggatctg aatgtaaaag gcttccaaca gtgggtggct gaggtgctgg ctctggtgcc
     7681 gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt tcactgatat
     7741 ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg cgtagcgatg
     7801 cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct atgcatgatg
     7861 caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag ttgattcagg
     7921 cggcactggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa ggatcttctt
     7981 tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt accgtgcaaa
     8041 gcagtcttga tgcgataaaa gatgattttg atgtgtttat cgattttacc cgtccggaag
     8101 gtacgctgaa ccatctcgct ttttgtcggc agcatggcaa agggatggtg atcggcacta
     8161 cggggtttga cgaagccggt aaacaggcaa ttcgtgacgc cgccgccgat attgcgattg
     8221 tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg gagaaagcag
     8281 ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat agacataaag
     8341 ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac gcccttgata
     8401 aagatctgaa agattgcgcg gtctacagtc gtgaaggcca taccggtgaa cgtgtgccag
     8461 gtaccattgg ttttgctacc gtgcgtgcag gtgacatcgt tggcgaacat accgcgatgt
     8521 ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgc atgacatttg
     8581 ctaacggtgc ggtaagatcg gctttgtggt tgagtggtaa ggaaagtggt ctttttgata
     8641 tgcgagatgt gcttgatctc aataatttgt aaccacaaaa
//
LOCUS       DAZOYF010000167         8642 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZOYF010000167 DAZOYF010000000
VERSION     DAZOYF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.168"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..91)
                     /locus_tag="ACHUOF_003513"
     CDS             complement(<1..91)
                     /locus_tag="ACHUOF_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612543.1"
                     /translation="MEFSFSPKRLVVAVAAALPLMASAADTPST"
     gene            complement(770..1966)
                     /gene="hemY"
                     /locus_tag="ACHUOF_003514"
     CDS             complement(770..1966)
                     /gene="hemY"
                     /locus_tag="ACHUOF_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR0612544.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1969..3180)
                     /gene="hemX"
                     /locus_tag="ACHUOF_003515"
     CDS             complement(1969..3180)
                     /gene="hemX"
                     /locus_tag="ACHUOF_003515"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR0612545.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(3202..3942)
                     /gene="hemD"
                     /locus_tag="ACHUOF_003516"
     CDS             complement(3202..3942)
                     /gene="hemD"
                     /locus_tag="ACHUOF_003516"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR0612546.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3939..4880)
                     /gene="hemC"
                     /locus_tag="ACHUOF_003517"
     CDS             complement(3939..4880)
                     /gene="hemC"
                     /locus_tag="ACHUOF_003517"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR0612547.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5267..7813
                     /gene="cyaA"
                     /locus_tag="ACHUOF_003518"
     CDS             5267..7813
                     /gene="cyaA"
                     /locus_tag="ACHUOF_003518"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR0612548.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7853..8173)
                     /gene="cyaY"
                     /locus_tag="ACHUOF_003519"
     CDS             complement(7853..8173)
                     /gene="cyaY"
                     /locus_tag="ACHUOF_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR0612549.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8221..>8642)
                     /locus_tag="ACHUOF_003520"
     CDS             complement(8221..>8642)
                     /locus_tag="ACHUOF_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR0612550.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREGNVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1967 a         2236 c         2223 g         2216 t
ORIGIN      
        1 cagttgacgg ggtatctgct gcgctggcca tgagaggaag agcggcggcg acagcaacaa
       61 caagacgttt gggtgaaaac gaaaattcca tgcaaaatgc tccggtttca tgtcatcaaa
      121 atgatgacgt aattaagcat tgataattga gatccctctc cctgacagga tgattacata
      181 aataatagtg acaaaaataa attatttatt tatccagaaa atgaattgga aaatcaggag
      241 agcgttttca atcctacctc tggcgcagtt gatatgtaag gcaggtttat tatatcgcgt
      301 tgattattga tgctgttttt agttttaacg gcaattaata tatgtgttat taattgaatg
      361 aattttatca ttcataataa gtatgtgtag gatcaagctc aggttaaata ttcactcagg
      421 aagttattac tcaggaagca aagaggatta cagaattatc tcataacaag tgttaaggga
      481 tgttatttcc cgattctctg tggcataata aacgagtaga tgctcattcc atctcttatg
      541 ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt gcttatcgtc
      601 cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg agtgaagcac
      661 ccacttgttg tcatacagac ctgttttaac gcctgctccg taataagagc aggcgttttt
      721 ttatgcatca gaaaggtccc ggaggtgctt tcctccgggt gagaaggaac tactgtggcg
      781 ggttattctg caacgttaac atcaaaccgt cgcgacgcat cgctgcagct tcttccggct
      841 tgtgcagtct gtccagcgcg tcggcaagcc atgcgtaatc gtaggcgtcc ggacgttgtt
      901 tcagcgccgc gcggaaggcg agcgatgctt cctgccattc accgtgcttc atcagtgact
      961 gacccaatgt gctccacaac agcgggcgat caccgacgtt tttgatttgc tggcgtagca
     1021 ctttttccag ctgttccggg ttattggttt tcagacgcgg gatcggcagc agtaggcgat
     1081 cgtcatactg gcgtttcagg ccgtcgatga taatttgctg ggcagtatca tgatcgtcac
     1141 attcaataag atgttccgcc attgccacct gcaacgctac ctgatgacgc gttttccggc
     1201 tttggttttt ccaccagtta cgcaaacctt cactaccgtt atcggcacgc gcctgatcca
     1261 tcaggccaat ccatgcctgt tgttccagca ttgcacgatg ttcttcatca ccaacatggg
     1321 ctttcgccat tgatgggatg atatccagca gagaactcca tgcccctgtg cggatatacg
     1381 cctgttccgc cagacgtaat acttcaggat ggcgtggcgt aacttccagc agcttatcca
     1441 cgccgtggcg tgcagcatgg ttttcattac gggccagttg cagacgtaca cgggtgattt
     1501 ctaccggaat ggtgtcattg ccggccagct ccgctgcgcg ttccagatgt tggttggcgc
     1561 gtgcttcatc accacgttgt tgcgcggctt cggcagccag tagatagttc accaccggtt
     1621 gttcagcgtg atcggcattt ttcgccatca gcttttcaac ttgctgataa tcaccttccg
     1681 ccagtttcag cagcgcctgt tcggtctgct tacgcgcacg gcgacgctta cgtccgacaa
     1741 accatccacg ggtgtgcgcg ccagtgcgga agatccgccg cagtagccac tcaatggcaa
     1801 acagcactac catcgccaga atcaatatga tcgccaggcc cgtgacgctg gtttcgatat
     1861 tgtagttgtc ggtctggatc agcacataac cctgatgacc ggcaatcatc gggccaacca
     1921 ctatccccgc aatcagcaaa acaaagagta ataacacttt tagcatggtt attctccttg
     1981 cggcgcggct gccggagtat caccttgcgg tgcaggtgca ggtgcaggtg caggtgcagg
     2041 tgcaggtgca ggtttagctt ccgttgtccc cgctgccggt tgtgccagca ggttacgcac
     2101 gcgagtctgc atcagttttt ccagcatcgc ctggctttgc agggtttccg gaagatccat
     2161 cgagatattt tgctggctta actggtccac ctcgtcgagg aacgctttgg tggtggcatc
     2221 atcagtatcg tagtaagcac gtacccaggt ggagacgttc tccagcgcct ggcgataagt
     2281 ctcttcctga tgacgcggta cagcttgtgc tgcgaccagc aggcgagagc gaatattttc
     2341 gcgcagatag atatcctgat ttggcgctaa cagcggtacg gcggtgtcat cacgacggcg
     2401 aatcgtaatg aagttgtcca taaagttctg ccagcttttt tgcagattga tacgccattc
     2461 gctgatggaa ctggaaagct cttcaccgtc tgaatccatc ggcgaaccat cgctgtcatt
     2521 atcggccaga cgcaggttat ctacctgatt tgaaagctga ttaagcttaa ggatgatgcc
     2581 gtcataatcc acctgcgata ctgcagaaag gctggcgata tcatcggtaa ttgcccgacg
     2641 aacggtaatc agactcgggt cattcatatc cgccaggctg gcgtctgcac ttttcagcaa
     2701 cgctgcagag gtcgtgacgt cctgatcgct ccacagcttc cgtccggcga gtttcaccag
     2761 aaaatcggcc tgagccagca gccaggtttt agcatcgctg ccggaaatgg tggcgatctt
     2821 ttgttggact tcatccaact gttttgccag cgtttcttgc tgacgattcg cctgcttaag
     2881 ctgcgcagct tgttgcttaa taatgccttc cagctcggct ttttggctct cctgggcttt
     2941 ttgcaatgcc gtcagttggt tagccagggc atcgctggtg gcggtctgat tgacggcctg
     3001 ttgtttaccc cagccataca aaccgacacc cgccgccaga gcaatagcga tagccaccgc
     3061 gctgagaatc aatgcagtat tgttcttact ctttttttct gttgcgacag gttgtgacgt
     3121 ggtgtccacg gcctccctgg tctcttcaac cacggcggag gttttttctt gttccgtcat
     3181 tatggcttcc tgttatgaga gttattgtaa tgcccgtaaa agcgcatcgt tgtcagcgtt
     3241 atcggcgacc ttaatgtctt gccagcccag ttcccgggcg agtttcgcca aacgctcact
     3301 gacgaccaat agtcgacagt gtagtaacca gtgctcacga taccattgtg ggatcagcga
     3361 ccagagttgc tgcaacattt caccgctggt aacaacgacc atcgtcacct cgcgggattg
     3421 ccagcgcatc gcttcttctg caccatcgta atggattgcg catcgttgat aacattcaca
     3481 aaaagtgacc tcagcaccgc gcaccgtcag ggtatcccca attagctcac gaccgccatt
     3541 gccacgtaat atcagcgcac gtttgcccgc aatattttgt aattcaggta attgtagcaa
     3601 gacttcgctg atttcccgat cctgtgggta gagaatcttc tgtccgctta cggtatgtag
     3661 cgccagtgcg gtggtgcgtc caatggcgaa ataagtaggt agttggggcc atttacgatc
     3721 ttgctgatgc agctgtgatt gggcaaaagc aaccgcgtgt tgcgagaggg caaacaacag
     3781 atcgctctcc cccagcgctg ccagttgatc agcaagttgc ggtaattgtc gacccggaga
     3841 aaactcaatc agcggaaaat gccaggccac ctgccccagt gtgcgcagac ggctcactaa
     3901 ctcttctcca gcgggagacg ggcgggtgac caggatactc atgccggggc ctctccgtta
     3961 tagacttcag cgaggatctc gcgcgcgccg ttattcagta gctcttctgc cagcgaaatc
     4021 cccatttgtt cggcatcttg cggcgcaccg cggcgttcac cgcgaataat ctgcgaaccg
     4081 tccggcgcgc cgaccagcgc acatagccag atttcgccat caataagctc ggcgtagcta
     4141 ccaattggca cctgacatcc gccttcgaga cgggtattca tggcgcgttc tgcggtaacg
     4201 cgcagtgcag tttcgtggtg attcagcgcg gcaagcagct cgcgagtgcg tgtatcatca
     4261 aggcggcatt caataccgac tgcaccttgt cctaccgccg gaagagaaat ctcgggtggt
     4321 aacgcggcgc gaatgcggga ctccagacct aaacgtttta gtccggctac ggcaagaatg
     4381 atggcatcgt attcgccgtt atctagtttg ctcaggcgag tgccgacgtt gccgcgcagg
     4441 gagcggataa tcagatccgg gcggcgttca gccagttggc actggcgacg taaactggac
     4501 gtcccgacga tactgcctgc tggtaacgca tccagactgt catagttatt ggacacaaag
     4561 gcatcgcgag gatcttcacg ctcgcaaata gtgaccagcc ccagaccttg cgggaattca
     4621 accggcacat ctttcattga atgtacggcg atatcagcgc gattctccag gagcgcgact
     4681 tccagctctt ttacaaataa gccttttccg cctactttcg ccagcggcgt atcaagaatc
     4741 acatcgccgc gcgtcaccat cggtaccagt tcaacgacca ggcccggatg gctcgccatc
     4801 aacttgtctt tgacatagtg tgcctgccag agtgcaagtg ggctttggcg tgtggcaatt
     4861 cttaaaacat tgtctaacat gcttgttacc gtcattatca tccgtggtcc atcctaacat
     4921 ccttgccaga gtgatgtcag tgttgtggtg aaacgtagac gcctgcgcaa accgtaaaat
     4981 gaggtctggc agtggatcct gacaggcgtt tcacgccgtc gtaataagga atttacagag
     5041 aataaacggt gctacacttg tatgtagcgc atctttcttt acggtcaatc agcaaggtgt
     5101 taaattgatc acgttttaga ccattttttc gtcgtgaaac taaaaaaacc aggcgcgaaa
     5161 agtggtaacg gttacctttg acatacgaaa tatcccgaat gccgcgtgtt accgttgatg
     5221 ttggcggaat cacagtcatg acgggtagca aatcaggcga tacgtcttgt acctctatat
     5281 tgagactctg aaacagagac tggatgccat aaatcaattg cgtgtggatc gcgcgcttgc
     5341 tgctatgggg cctgcattcc aacaggtcta cagtctactg ccgacattgt tgcactatca
     5401 ccatccgcga atgccgggtt accttgatgg taacgttccc aaaggcattt gcctttacac
     5461 gcctgatgaa actcaacgcc actacctgaa cgagcttgaa ctgtatcgtg gaatgtcagt
     5521 acaggatcca ccgaaaggtg agcttccaat tactggtgta tacaccatgg gcagcacctc
     5581 gtccgtaggg caaagttgtt cctctgacct ggatatctgg gtctgtcatc aatcctggct
     5641 cgatagcgaa gagcgccagt tactacaacg taaatgcagc ctgctggaaa gctgggccgc
     5701 ctcgctgggt gtggaagtca gcttcttcct gattgatgaa aaccgcttcc gtcataatga
     5761 aagcggcagc ctggggggcg aagattgtgg ctccacccag catatactgc tgcttgacga
     5821 attttatcgt actgccgtgc gtctcgccgg taagcgtatt ctgtggaata tggtgccgtg
     5881 cgacgaagaa gagcattacg acgactatgt gatgacgctt tacgcgcagg gcgtgctgac
     5941 gccaaatgaa tggctggatc tcggtggctt aagctcgctt tctgctgaag agtactttgg
     6001 tgccagcctt tggcagctct acaagagtat cgattcccca tacaaagcgg tgctgaaaac
     6061 actgctgctg gaagcctatt cctgggaata cccgaaccca cgtctgctgg cgaaagatat
     6121 caaacagcgt ttgcacgacg gcgagattgt atcgtttggc ctcgatccat actgcatgat
     6181 gctggagcgt gttactgaat acctgacggc gattgaagat ttcacccgtc tggatttagt
     6241 acgtcgctgc ttctatttaa aagtgtgcga aaaactcagc cgtgaacgcg cctgtgtagg
     6301 ctggcgtcgc gcagtgttga gccagttagt gagcgagtgg ggttgggacg aagctcgtct
     6361 ggcaatgctc gataaccgtg ctaactggaa gattgatcag gtgcgtgagg cgcacaacga
     6421 gctgctcgac gcgatgatgc agagctaccg taatctgatc cgctttgcgc gtcgcaataa
     6481 ccttagcgtc tccgccagtc cgcaggatat cggcgtactg acgcgtaaac tgtatgccgc
     6541 gtttgaagca ttgccaggta aagtgacgct ggtaaacccg cagatttcac ccgatctctc
     6601 ggaaccgaat ctgaccttta tttatgtgcc gccgggccgg gctaaccgtt caggttggta
     6661 tctgtataac cgcgcgccaa atattgagtc gatcatcagc catcagccgc tggaatataa
     6721 ccgttacctg aataaactgg tggcgtgggc atggtttaac ggcctgctga cctcgcgcac
     6781 ccgtttgtat attaaaggta acggcattgt cgatttgcct aagttgcagg agatggtcgc
     6841 cgacgtgtcg caccatttcc cgctgcgctt acctgcaccg acaccgaagg cgctctacag
     6901 cccgtgtgag attcgccatc tggcgattat cgttaacctg gaatatgacc cgacagctgc
     6961 gttccgcaat caggtggtgc attttgattt ccgtaagctg gatgtcttca gctttggcga
     7021 gaatcaaaat tgcctggtag gtagcgttga cctgctgtac cgcaactcgt ggaacgaagt
     7081 gcgtacgctg cacttcaacg gcgagcaatc gatgatcgaa gccctgaaaa ctattctcgg
     7141 caaaatgcat caggacgccg caccgccaga tagcgtggaa gtcttctgtt atagccagca
     7201 tctgcgcggc ttaatccgta ctcgcgtgca gcaactggtt tctgagtgta ttgaactgcg
     7261 cctttccagc acccgccagg aaaccgggcg tttcaaggcg ctgcgcgttt ctggtcaaac
     7321 ctggggcttg ttcttcgaac gcctgaatgt atcggtacag aagctggaga acgccatcga
     7381 gttttatggc gctatttcgc ataacaaact gcacggcttg tcagtgcagg ttgaaaccaa
     7441 tcacgtcaaa ttaccggcgg tggtggacgg ctttgccagc gaagggatca tccagttctt
     7501 tttcgaagaa acgcaagacg agaatggctt taatatctac attctcgacg aaagcaaccg
     7561 ggttgaggtg tatcaccact gcgaaggcag caaagaggag ctggtacgtg acgtcagtcg
     7621 cttctactcg tcatcgcacg accgcttcac ctacggctca agcttcatca acttcaacct
     7681 gccgcagttc tatcagattg tgaaggttga tggtcgcgaa caggtgattc cgttccgaac
     7741 aaaatcgatt ggcaacctgc cgcccgccaa tcaggatcac gatacgccgc tattacagca
     7801 gtatttttcg tgatgagcgt gccggaaagc gaggcttatc cggcatgcaa tcttagcgga
     7861 aactgactgt ttcacccgcc tgttgcgtcg ccgcctgttc cagcaaatcc cagaaggttt
     7921 cgccgctgcg atcgcaaatc cattcatcgc ctttcaggtc aaaatggtag ccgccctgtt
     7981 tggttgccag ccacacctgg tgcagcggct cctggcggtt aataatgatt ttgctgccgt
     8041 tttcaaaggt aatggtcagt acgccgccgt tgatttcgca gtcgatatcg ctgtcgccat
     8101 ccctgtcgtc cagacgctct tcaatggtca gccacagttg atcagccagg cgatgaaatt
     8161 cactgtcgtt cattgttgta tcctgttttt aagtgatggc ggcagtatag cggcatgggg
     8221 tcagggcttc aaagtttgca cctctgctgc tgcgttccgg catgattcat ccgtcaccgg
     8281 aataatgatg tctctgtttt gaacttcacg tacgttcccc tctctgacac gagttttcgc
     8341 cgggcagggg gagggcgtaa tccttacctc tgtatcggtc catgtataaa cattgcgatc
     8401 gtcataagcg aagcgatcgt ttttagttag cgaaccgtta actggaataa tttgcaagct
     8461 ctttgaccgt aattggcgga taaagtaagc atgctgtttg ccgctattaa atgagcgatc
     8521 aatgaccagg tctggcgtat ccagcgggat tatctcaagc tggtcttcac caaatgtcga
     8581 taattttgtt acatacagcc gatctttata ttgcgcaata ttgccagtaa tcagatggaa
     8641 tt
//
LOCUS       DAZOYF010000168         8528 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZOYF010000168 DAZOYF010000000
VERSION     DAZOYF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.169"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..189
                     /gene="csgC"
                     /locus_tag="ACHUOF_003521"
     CDS             <1..189
                     /gene="csgC"
                     /locus_tag="ACHUOF_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR0612551.1"
                     /translation="GSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVS
                     DGQALHLSQQWTPSSEKS"
     gene            310..621
                     /gene="ymdA"
                     /locus_tag="ACHUOF_003522"
     CDS             310..621
                     /gene="ymdA"
                     /locus_tag="ACHUOF_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR0612552.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            716..1249
                     /gene="ymdB"
                     /locus_tag="ACHUOF_003523"
     CDS             716..1249
                     /gene="ymdB"
                     /locus_tag="ACHUOF_003523"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR0612553.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1191..2672
                     /gene="clsC"
                     /locus_tag="ACHUOF_003524"
     CDS             1191..2672
                     /gene="clsC"
                     /locus_tag="ACHUOF_003524"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR0612554.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2680..3837)
                     /gene="mdoC"
                     /locus_tag="ACHUOF_003525"
     CDS             complement(2680..3837)
                     /gene="mdoC"
                     /locus_tag="ACHUOF_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR0612555.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4231..5766
                     /gene="mdoG"
                     /locus_tag="ACHUOF_003526"
     CDS             4231..5766
                     /gene="mdoG"
                     /locus_tag="ACHUOF_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR0612556.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5759..8302
                     /gene="mdoH"
                     /locus_tag="ACHUOF_003527"
     CDS             5759..8302
                     /gene="mdoH"
                     /locus_tag="ACHUOF_003527"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR0612557.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2150 a         2025 c         2222 g         2131 t
ORIGIN      
        1 ggcagttcag ggcaaagtca gacgaagcaa gaaaagaccc tctcattgcc tgctaatcaa
       61 cccattgctt tgacgaagtt gagtttaaat atttccccgg aagatcgggt gaaaatagtt
      121 gttactgttt ctgatggaca ggcacttcat ttatcacaac aatggacgcc ctcttcagaa
      181 aagtcttaat ttgttgaaat atcgagcata agatgaatct ggagagaatg gtctgctgcg
      241 aatcagccaa cctgaaagta tggataacac aaccttcaag gatgactaat cattgaggaa
      301 atagaataaa tgttcagacc ttttttagac tctcttatgc tcggcagttt gttttttcct
      361 tttattgcca ttgcaggaag caccgcgcaa gggggcgtga tccattttta tggccaaatt
      421 gtggaaccgg catgtgacgt cagcacccag tcatcacccg tagaaatgaa ctgcccacag
      481 aatggttcta ttccgggcaa aacctactcc agcaaagcgt taatgagcgg caatgtcaaa
      541 aacgcgcaaa tagcctcagt aaaagtgcag tatcttgata aacagaaaaa gctggctgta
      601 atgaacatcg aatataacta agtttctgaa caactcacgt tgctgagcag aaaaatgcga
      661 tttaaccaaa aagcctgctg tacacttaag aaacaagaag gtgtaaggag gcgttatgaa
      721 aacgcgtatt catgttgtgc agggtgatat taccaaactg gccgttgatg tgattgtgaa
      781 tgcggctaat ccgtcattaa tgggaggcgg cggcgtcgat ggggccattc atcgcgcagc
      841 gggtccggcc ctgctggatg cctgtttaaa agtcaggcaa cagcagggcg attgccctac
      901 ggggcatgcc gttattacgc ttgcaggcga tcttcccgct aaagccgtag tacacaccgt
      961 cgggccagtc tggcgtggtg gggaacaaaa cgaagaccag cttttgcagg atgcctatct
     1021 gaatagccta cgactggtgg cggcaaacag ctatacgtca gtggcttttc ctgcaatcag
     1081 tactggggtt tatagttacc ctcgtgcggc agcggctgaa atcgcagtta aaaccgtttc
     1141 agaatttatt acccgtcacg ctttacccga acaggtatac tttgtctgtt atgatgaaga
     1201 aaacgcccac ctctacgaaa gactccttac ccaacaagga gatgaatgat ttgccccggc
     1261 tggcgagcgc ggtgctgcca ctgtgttcgc aacatcccgg tcagtgtggt cttttccctc
     1321 tggagaaaag tctggatgcg tttgccgccc ggtatcgtct ggccgaaatg gcagagcata
     1381 cgctggatgt tcagtattac atctggcagg acgatatgtc ggggcggtta ctgttttccg
     1441 ccctgttagc cgcagcaaag cgtggcgttc gcgtccgttt gttgctggac gacaacaata
     1501 cgcccggact tgacgacatt ttgcgcttgc ttgacagtca tccacgcatt gaagtccggc
     1561 tttttaatcc tttctcgttt cgcttgctac gtccgcttgg atatatcacc gacttttccc
     1621 gtctcaatcg ccggatgcac aataaaagtt tcactgtcga tggcgtggtt accctagtgg
     1681 gaggacgaaa tattggtgat gcctattttg gtgcagggga agagccactt ttttcggatt
     1741 tagatgtcat ggcaataggc cccgtggtag aggatgttgc cgatgatttc gcccgctact
     1801 ggtattgcaa atcggtttca cccttacagc aggtgctgga tgtcccggaa ggtgaaatgg
     1861 cggatcgcat cgagttaccc gcctcctggc ataacgatgc catgacgcat cgttatttac
     1921 gcaaaatgga atccagtcca tttataaatc atctggttga tggaacgttg ccgcttatct
     1981 gggcgaagac acgtttatta agtgatgatc cggcgaaagg ggagggcaag gcaaaacggc
     2041 attcactgtt accgcagcgc ctgttcgata tcatgggctc acccagtgaa cgcatcgata
     2101 ttatctcttc ctattttgta ccgacacgcg caggtgtggc gcaactctta cggatggtga
     2161 gaaaaggggt aaagattgcg atcctaacca attctcttgc cgctaacgat gttgctgtcg
     2221 tccatgccgg atacgcgcgc tggcgcaaaa aattgctccg ctatggcgtg gaattatatg
     2281 aactcaagcc gacgcgtgaa caaagcagta cgttacacga tcgcggcata accggtaatt
     2341 ccggtgccag cctgcatgct aaaactttta gcatcgatgg taaaacggtg tttatcggct
     2401 ctttcaattt cgatccgcgt tcaacattgc tcaatactga aatgggcttc gtgatagaga
     2461 gcgaaacgct ggcacagtta attgataaac gctttattca gagccagtac gatgcggcct
     2521 ggcagctccg tctggacagg tggggacgga tcaactgggt tgatcgtcat gcaaagaaag
     2581 aggttattct caaaaaagaa cccgccacca gtttctggaa gcgggttatg gtcagactgg
     2641 cgtcgatatt gcccgtggaa tggttattgt aaatgtggct taacgggctg gtgctttatc
     2701 gttctcacgc ttaacaaccg gtttaccaga gaacaaaaac ttcagtaacg ggatgcgtaa
     2761 atgaatttca tacagaatta tcgcaatccc tactacgaat atcaggccac agagaaaacc
     2821 aagccagttg gaggtgatgt gcggtgtaat gtatgcgccg aaaaacagcg ttaacgggtg
     2881 gtgaaccaga tagataaaca gcgatgcgtt aacaaaataa gtcacccgcg ctgactggaa
     2941 gttaagcaaa cggtggccaa aggagaagac cacattcacc atccacaggc cgaggaccat
     3001 ggtgatcacc gactcggttt cgtacatcca ggcatcgcca ctgccatagc gctggttgag
     3061 taaataagcg acaaacgcca atgctgcagc gagggtacag ccacgagacg gcgtggtaaa
     3121 caaggcttta agatgaggga aaatgaaagc cagtgcgccg aggataaaga acggtaaata
     3181 aaatagcgtt tgcatgacaa taaaattgaa catgccatta ctcagaatgg gtggatacac
     3241 aataaaaatc gttcttctta ttaccgcata gccgatgccg aggcataaaa aaatcaccga
     3301 tagttttacc atcgagaatt ttttattcgt tttatcagaa ttttctaaat tatttctgat
     3361 gcgcttaaat atccatacac acagcgtcgt catgactact aacaccagta aaaaccacag
     3421 gtgtgatatt aattcccagg ccaacgtatt atatttgtca tacaatgata gcccaggcca
     3481 actttccgct ttccctttga catattgcag cataataaat tgcggtaatg tcagtagggg
     3541 gatggctgtt aacatcggga tacctacacg ttcgacacgt actttccacc attttttcaa
     3601 gggatagcgt aaaaaaagca tgtaggaaaa gtatccggat ataacgaaaa atacctgcat
     3661 gcggaacgag tggatgaagt cattaaaaag ggttagccac aatgacggtt cggcgctatt
     3721 cacatgccat gtatggctcg aatagattaa agaaatatga aaagggatcc ctaacaacat
     3781 cagccaggcg cggatggagt cgaggaaata ttcacgttgc gcgggtactg gattcatata
     3841 tggttaacta atctcggatt tttcgtctta tccctgtcgg gttatgcctt taggcttgtt
     3901 gccatagcga caccgacctg accgcgccag gcgcaggctt caaggttttt atgcatagca
     3961 tcatcgctac cactaaccag aatggaagcg tctgtaagac ggttgataaa taaatttgct
     4021 ggcaaaccct acacgaagtc gatgcttctg tctttaggag aagcacggaa agtgaaaacg
     4081 gttgcaatca ggtgcttaat ccatgagcca gcgtgctgaa cgataccggg attcagttgt
     4141 cggaatggct ggttatccat taaaatagat cggatcgata taagcacaca aagggggaag
     4201 tgcttactaa ttatgaaaca taaactacaa atgatgaaaa tgcgttggtt gagtgctgca
     4261 gtaatgttaa ccctgtatac atcttcaagc tgggctttca gtattgatga tgtcgcaaag
     4321 caagctcaat ccttagccgg gaaaggctat gaggcgccta aaagcaactt gccctccgtt
     4381 ttccgcgaca tgaaatacgc ggactatcag cagatccagt ttaatcatga caaagcgtac
     4441 tggaacaatc tgaagacccc attcaaactc gagttctacc atcagggtat gtacttcgat
     4501 accccggtca aaataaatga agtgactgcc accgcagtca aacgaatcaa atacagcccg
     4561 gattatttca ctttcggcga tgttcagcat gacaaagaca cggtaaaaga ccttggtttt
     4621 gccggtttta aagtgcttta cccgatcaac agcaaagata aaaacgatga aatcgtcagc
     4681 atgctcgggg ccagctattt ccgcgtgatt ggtgcaggtc aggtttatgg cctttctgcc
     4741 cgcggcctag caattgatac cgctttgcca tcgggtgaag aatttccacg cttcaaagag
     4801 ttctggatcg agcgtccaaa accgactgat aaacgtttaa ccatttatgc attgcttgac
     4861 tcgccgcgtg cgacaggtgc ttacaaattc gtggttatgc cagggcgtga cacggttgtg
     4921 gatgtgcagt cgaaaatcta tctgcgcgat aaagtcggca aactgggggt tgcaccgtta
     4981 accagtatgt tcctgtttgg gccgaaccaa ccgtcgcctg caaataacta tcgtccggag
     5041 ttgcacgact ctaacggtct gtctatccat gctggtaatg gcgaatggat ctggcgtccg
     5101 ttgaataacc cgaaacattt agcggtcagc agcttctcca tggaaaaccc gcgaggcttt
     5161 ggtctgttgc aacgaggtcg tgatttctcc cgctttgaag atctcgatga tcgttacgat
     5221 ctccgtccaa gcgcatgggt gactccgaaa ggggagtggg gcaaaggcag cgttgagctg
     5281 gtggaaattc caaccaacga tgaaaccaac gataacatcg tcgcttactg gacgccggat
     5341 cagctgccgg agccgggtaa agagatgaac tttaaataca ccatcacctt cagccgtgat
     5401 gaagacaaac tgcatgcgcc agataacgca tgggtgcaac aaacgcgtcg ttcaacgggg
     5461 gatgtgaagc agtcgaacct gattcgccag cctgacggta ctatcgcctt tgtggtcgat
     5521 tttaccggcg cagagatgaa aaaactgcca gaggataccc cggtcacagc gcaaaccagc
     5581 attggtgata atggtgagat agttgaaagc acggtgcgct ataacccggt taccaaaggc
     5641 tggcgtctgg tgatgcgtgt gaaagtgaaa gatgccaaga aaatcactga aatgcgtgct
     5701 gcgctggtga atgccgatca gacgttgagt gaaacctgga gctaccagtt acctgccaat
     5761 gaataagaca actgagtaca ttgacgcaat gcccatcgcc gcaagcgaga aagcagcatt
     5821 gccgaagact gatatccgcg ccgttcatca ggcgctggat gccgaacacc gcacctgggc
     5881 gcgggaggat gattccccgc aaggctcggt aaaggcgcgt ctggaacaag cctggccaga
     5941 ttcacttgct gatggacagt taattaaaga cgacgaaggg cgcgatcagc ttaaggcgat
     6001 gccagaagca aaacgctcct cgatgtttcc cgacccgtgg cgtaccaacc cggtaggccg
     6061 tttctgggat cgcctgcgtg gacgcgatgt cacgccgcgc tatctggctc gtttgaccaa
     6121 agaagagcag gagagcgagc aaaagtggcg taccgttggt accatccgcc gttacattct
     6181 gttgatcctg acgctcgcgc aaactgttgt cgcgacctgg tatatgaaga ccattcttcc
     6241 ttatcagggt tgggcgctga ttaatcctat ggatatggtt ggtcaggatt tgtgggtttc
     6301 ctttatgcag cttctgcctt atatgctgca aaccggtatc ctgatccttt ttgcggtact
     6361 gttctgttgg gtgtccgccg gattctggac ggcgttgatg ggcttcctgc aactgcttat
     6421 tggtcgcgat aaatacagta tatctgcgtc aacagttggc gatgaaccat taaacccgga
     6481 gcatcgcacg gcgttgatca tgcctatctg taacgaagac gtgaaccgtg tttttgctgg
     6541 cttgcgtgca acgtgggaat cagtaaaagc caccgggaat gccaaacact ttgatgtcta
     6601 cattctgagt gacagttata acccggatat ctgcgtcgca gagcaaaaag cctggatgga
     6661 gcttatcgct gaagtcggtg gcgaaggtca gattttctat cgccgccgcc gtcgccgcgt
     6721 gaagcgtaaa agcggtaata tcgatgactt ctgccgtcgc tggggcagcc agtacagcta
     6781 catggtggtg ctggatgctg actcggtaat gaccggtgat tgtttatgcg gcctggtgcg
     6841 cttgatggaa gccaacccga acgccgggat cattcagtcg tcgccgaaag cgtccggtat
     6901 ggatacgctg tatgcgcgct gtcagcagtt cgcgacccgc gtgtatgggc cactgtttac
     6961 agccggtttg cacttctggc aacttggcga gtcgcactac tggggacata acgcgattat
     7021 ccgcgtgaaa ccatttatcg agcactgcgc actggctccg ctgccgggcg aaggttcctt
     7081 tgccggttca atcctgtcac atgacttcgt agaagcggcg ttgatgcgcc gtgcaggttg
     7141 gggggtctgg attgcttacg atctcccggg ttcttatgaa gaattgccgc ctaacttgct
     7201 tgatgagcta aaacgtgacc gccgctggtg ccacggtaac ctgatgaact tccgtctgtt
     7261 cctggttaag ggtatgcacc cggttcaccg tgcggtgttc ctgacgggcg tgatgtctta
     7321 tctctccgct ccgctgtggt ttatgttcct cgcgctctct actgcattgc aggtagtgca
     7381 tgcgttgacc gaaccgcaat acttcctgca accacggcag ttgttcccgg tgtggccgca
     7441 gtggcgtcct gagctggcga ttgcactttt tgcttcgacc atggtgctgt tgttcctgcc
     7501 gaagttattg agcattttgc ttatctggtg caaaggaacg aaagaatacg gcggcttctg
     7561 gcgcgttaca ttatcgttgc tgctggaagt gctgttttcc gtgctgctgg ctccggtacg
     7621 catgctgttc catacggtct tcgttgtcag cgcgttcctt ggctgggaag tggtgtggaa
     7681 ttcaccgcag cgtgatgatg actccacttc atggggcgaa gcgttcaaac gccacggctc
     7741 acagctgctg ttggggttag tgtgggctgt tgggatggcg tggctggatc tgcgtttcct
     7801 gttctggctg gcaccgattg tcttctcgtt gatcctgtca ccgtttgttt cggtgatttc
     7861 cagccgtgcc accgttggtc tgcgcaccaa acgctggaaa ctgttcctga tcccggaaga
     7921 gtattcaccg ccgcaggtgc tggttgatac cgatcggttc cttgagatga atcgccaacg
     7981 ctcccttgac gatggcttta tgcacgcagt gtttaacccg tcatttaacg ctctggcaac
     8041 cgcaatggcg acagcgcgtc accgcgccag taaggtgctg gaaatcgccc gtgaccgcca
     8101 cgttgaacag gcgcttaacg agacgccaga gaagctgaat cgcgatcgtc gcctggtgct
     8161 gctaagcgat ccggtgacga tggcccgtct gcatttccgc gtctggaatt cccctgaaag
     8221 atattcttca tgggtgagtt attacgaagg gataaagctc aatccactgg cattgcgtaa
     8281 accggatgcg gcttcgcaat aaaaacgtag ttgcctgatg cgctacgctt atcaggccta
     8341 catcgttcct gcaatttatt gattttgcaa gattttgtag gtcggataag gcgttcacgc
     8401 cacatccggc aaaaacaacg agccaataaa aataccggcg ttatgccgta atgccagtca
     8461 gttaagcaac tgactggctc tttttcgggg ctgtggggta tttccagggc ctctccttta
     8521 ccactctc
//
LOCUS       DAZOYF010000169         8521 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZOYF010000169 DAZOYF010000000
VERSION     DAZOYF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.170"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..181
                     /locus_tag="ACHUOF_003528"
     CDS             <1..181
                     /locus_tag="ACHUOF_003528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612558.1"
                     /translation="ILFFIKTAFHFYGFVYTDGLCGNCRIEQVCATLGSVLKSKKIYL
                     LERFTVFFIRLNMQR"
     gene            235..2613
                     /gene="ppsA"
                     /locus_tag="ACHUOF_003529"
     CDS             235..2613
                     /gene="ppsA"
                     /locus_tag="ACHUOF_003529"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR0612559.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2670..4316)
                     /gene="fadK"
                     /locus_tag="ACHUOF_003530"
     CDS             complement(2670..4316)
                     /gene="fadK"
                     /locus_tag="ACHUOF_003530"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFR0612560.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(4373..4666)
                     /locus_tag="ACHUOF_003531"
     CDS             complement(4373..4666)
                     /locus_tag="ACHUOF_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR0612561.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4663..5952)
                     /locus_tag="ACHUOF_003532"
     CDS             complement(4663..5952)
                     /locus_tag="ACHUOF_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0612562.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(6008..6946)
                     /locus_tag="ACHUOF_003533"
     CDS             complement(6008..6946)
                     /locus_tag="ACHUOF_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR0612563.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6966..7730)
                     /locus_tag="ACHUOF_003534"
     CDS             complement(6966..7730)
                     /locus_tag="ACHUOF_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR0612564.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            8049..>8521
                     /locus_tag="ACHUOF_003535"
     CDS             8049..>8521
                     /locus_tag="ACHUOF_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFR0612565.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         2036 a         2185 c         2211 g         2089 t
ORIGIN      
        1 cattttattt tttataaaaa cagcatttca tttttatggt ttcgtttata ccgatggttt
       61 atgtggaaat tgtcgaatag agcaggtttg cgcaacgctg ggatcagtct taaaaagtaa
      121 aaaaatatat ttgcttgaac gattcaccgt ttttttcatc cggttaaata tgcaaagata
      181 aatgcgcaga aatgtgtttc tcaaaccgtt catttatcac aaaaggattg ttcgatgtcc
      241 aacaatggct cgtcaccgct ggtgctttgg tataaccaac tcggcatgaa tgatgtagac
      301 agggttgggg gcaaaaatgc ctccctgggt gaaatgatta ctaatctttc cggaatgggt
      361 gtttccgttc cgaatggttt cgccacaacc gccgacgcgt ttaaccagtt tctggaccaa
      421 agcggcgtaa accagcgcat ttatgaactg ctggataaaa cggatattga cgatgttact
      481 cagcttgcga aagcgggcgc gcaaatccgc cagtggatta tcgacactcc cttccagcct
      541 gagctggaaa acgccatacg cgaagcctat gcacagcttt ccgccgatga cgaaaacgcc
      601 tcttttgcgg tacgctcctc cgccaccgca gaagacatgc cggacgcttc ctttgccggt
      661 cagcaggaaa ccttcctcaa cgttcagggt tttgacgccg ttctcgtggc agtgaaacat
      721 gtatttgctt ctctgtttaa cgatcgcgcc atctcttatc gtgtgcacca gggttacgat
      781 caccgtggcg tggcgctctc cgccggtgtt cagcggatgg tgcgctccga cctcgcatct
      841 tctggcgtga tgttctccat tgataccgaa tccggctttg accaggtggt gtttatcact
      901 tccgcatggg gccttgggga aatggtcgtg cagggtgcgg ttaacccgga tgagttttac
      961 gtgcataaac cgacactggc ggcgaatcgc ccggctatcg tgcgccgcac catggggtcg
     1021 aaaaaaatcc gcatggttta cgcgccgacc caggagcacg gcaagcaggt taaaatcgaa
     1081 gacgtaccgc aggaacagcg tgacatcttc tcgctgacca acgaagaagt gcaggaactg
     1141 gcaaaacagg ccgtacaaat tgagaaacac tacggtcgcc caatggatat tgagtgggcg
     1201 aaagatggcc acaccggcaa actgttcatt gtgcaggcgc gtccggaaac cgtgcgctca
     1261 cgcggtcagg tcatggagcg ttatacgctg cattcacagg gtaagattat cgccgaaggc
     1321 cgtgctatcg gtcatcgcat cggcgcgggt ccggtgaaag tcatccatga catcagcgaa
     1381 atgaaccgca tcgaacctgg cgacgtgctg gttactgaca tgaccgaccc ggactgggaa
     1441 ccgatcatga agaaagcatc tgccatcgtc accaaccgtg gcggtcgtac ctgtcacgcg
     1501 gcgatcatcg ctcgtgaact gggcattccg gcggtagtgg gctgtggaga tgcaacagaa
     1561 cggatgaaag acggtgagaa cgtcactgtt tcttgtgccg aaggtgatac cggttacgtc
     1621 tatgcggagt tgctggaatt tagcgtgaaa agctccagcg tagaaacgat gccggatctg
     1681 ccgttgaaag tgatgatgaa cgtcggtaac ccggaccgtg ctttcgactt cgcctgcctg
     1741 ccgagcgaag gcgtgggcct tgcgcgtctg gaatttatca tcaaccgtat gattggcgtc
     1801 cacccacgcg cactgcttga gtttgacgat caggaaccgc agttgcaaaa cgaaatccgc
     1861 gagatgatga aaggttttga ttctccgcgt gaattttacg ttggtcgtct gactgaaggg
     1921 atcgcgacgc tgggtgccgc gttttatccg aagcgcgtca ttgtccgtct ctctgatttt
     1981 aaatcgaacg aatatgccaa cctggtcggt ggtgagcgtt acgagccaga tgaagagaac
     2041 ccgatgctcg gcttccgtgg tgcgggccgc tatgtttccg acagcttccg cgactgcttc
     2101 gcactggagt gtgaagcagt gaaacgtgtg cgcaacgaca tggggctgac caacgttgag
     2161 atcatgatcc cgttcgtgcg taccgtagat caggcgaaag cggtggttga agaactggcg
     2221 cgtcaggggc tgaaacgtgg cgagaacggg ctgaaaatca tcatgatgtg tgaaatcccg
     2281 tccaacgcct tgctggccga gcagttcctc gaatatttcg acggcttctc aattggctca
     2341 aacgatatga cgcagctggc gctcggtctg gaccgtgact ccggcgtggt gtctgaattg
     2401 ttcgatgagc gcaacgatgc ggtgaaagca ctgctgtcga tggctatccg tgccgcgaag
     2461 aaacagggca aatatgtcgg gatttgcggt cagggtccgt ccgaccacga agactttgcc
     2521 gcatggttga tggaagaggg gatcgatagc ctgtctctga acccggacac cgtggtgcaa
     2581 acctggttaa gcctggctga actgaagaaa taaaataaat ccccggcggc gttcagtcgc
     2641 cggggttatg tgatccccga agatgaaact tattcaatct cttcacagac atcctgcgtt
     2701 aatcgccgta taatatcttt tcttaacaaa aacttttgta ttttatctga ggcagtgcgc
     2761 ggtagttttt cgattaccac gatatgttca ggatatttat attttgcgac ccgtttacgg
     2821 ctaaaaaaag ccgccatctc ttccagcgat aatgaatgat gcggcgcttt cagcacgaca
     2881 taagcgcatg atcgttcacc taaacgttca tcgggcattg caaccacaca ggcatcgtga
     2941 attttaggat gctgcaataa aatatcttcc acttcacggc tgctaatatt ttcgccgccg
     3001 cggacaataa tatctttttt gcgtccggta atttttatat agccagcctc atccatacgg
     3061 cagagatcgc cgctgtaata ccagccttct tcatccagcg cacgggcggt taattcaggt
     3121 tcatcaaaat accccataaa cacattgggg ccacgcgatg cttcttcacc ttcgcaacct
     3181 ggcggtaagg tcttgcgtgc gtcatcgacc actttaatct ctacacctgc ggcagcgtaa
     3241 ccatcggtat gcataaagcg cgacaaagaa ttatcgagat tcaccaccgc atgcggcgaa
     3301 ctttctgtgg aaccataaac acttaataat ttaatgctgc gctgctggca ttcgcgcgcc
     3361 acttttttgg ggattgtggt accgccgcaa agaaagaagc gcagcgctga aaggtctgcg
     3421 ggttgtttct ctagtaaatt caaaagatca tagacaaacg gcgttgcgcc tagcatacag
     3481 gtgcaacgct gctgctcaag cagcgcgaga cacgcatcag gagtgaaaat atctaacaac
     3541 acgctgcgag cgccaattaa gaatggtgcc gttacgccat gcagaaagcc cgttgcgtga
     3601 ccaagtggcg caggcatcat aaagacatcc agccaggtca gattcagccg cgcgcaataa
     3661 gcccgctcac tggcgagaat attgttatgc gttagcatca cgccctttgg cagaccctcg
     3721 gttccggagg taaacagcac cgcagctaat tcatcgccgt gggtcgttat cgccgtggtc
     3781 agtgggatat tgtcggcgat aatctgactt aatgagaggg aagaggtggc gggagccagt
     3841 ttgtccacgc cgacaatttg ttgtagttgt ggaagctgat tttgcagcgg caggattaaa
     3901 tctaccggac gcgtttgttt aaacaacgtc ggtgcaaaga acatttttgc ctgacactta
     3961 ttgagcaccc acaccagttc tgcttcccgc caggaaggca acagcggcac ggaaaccgca
     4021 ccgattttca ggcaggcaag atagataacg gtaaattcac accagccagg cagttgaaat
     4081 gcgatgcgat cgcctgattc aatacccttc gctaacatcc agtttgccag acagctcgcg
     4141 gcgtgatcga gcgcgctata ggtgtacgat gcaccatgat tatcgaccac ggcaattttg
     4201 tctggcatcg cacgagcggt ctgctgccag taatcggcca gtgaagcatc gccccatagc
     4261 ccttgctgac gatacgccgc acgacgttgt tcgttaaacg ttaatgtcac tttcattttg
     4321 gactctcgaa acagaacatc aggcccgagt tgcgggcctg tgggatgcat gattagccgt
     4381 agcgaaaatc aataccgaag gtgcctgcgg gatattgcca ctgttcgaga atagtgttac
     4441 cgcacagcac ccgacaggtg ccgcactcca gacaaccggc gggatcaaaa tgaatgtttc
     4501 ctacgtcatc ctgcttataa agtccggcag ggcaggcttt cattaattta cggaattcat
     4561 tgatatcggg attttccgtc agaatgatat gcgggtggcc ctcatcaaca tggaatttat
     4621 tgacgcctaa tttgatgtcg acgttaaccg tagcgttctg gctcatagcg cggttgctcc
     4681 cttaatgcca tctttcagca agttgatcag cccaattttc ttcgcgtgtc ccatgatcat
     4741 tttgcgtacc ggctggtttg gtttgccgtc aatggtgaac atctcgttca tgatgtcggc
     4801 taccattcgt gggtattggc taaacaggcg cgggttttcc atcagcgccg ggatcttgcg
     4861 aaaatgctgc atatcacgca tgacgcagct ttgttccagc tcacgtttgt attgcgccag
     4921 actgttggcg gagaaatccg cgcgttcttt ggcggcgatc accgttgtgg cggcagcctg
     4981 agccgatgca atggctaaat ccatgccgcg gaccgtaaaa cccaaattca ggcagaagcc
     5041 tgcggcgtca ccaacgatca tcacgccctc gttaaccagt tgcggcacca ttgccagacc
     5101 gccttccggc accatatgcg cggaatattc aagcagtttg ccgccgctaa tcagcgggcg
     5161 aatggcgggg tgttgtttaa aatcttccag catttgcggc acgctttttt gcgcatgggc
     5221 gatatcaccc aatccacaaa ccagccccaa ggatatggaa tccttgttgg tatagagaaa
     5281 tcccccgccc atcaggccgt cagaagggga accggcaaac agccaggcgg caccttcctc
     5341 gcccgtaata ttaaagcgat cgttgatctg ttctggtgtg aggccaataa cctctttaac
     5401 accaacagcg taatgatgcg gatcggaagc gggaaccatt cccagcgagc ggccaagcat
     5461 cgagttaacg ccatcagcca gaatcaccac attcgcttcg agaatatcat ccccagcctg
     5521 cacgccagtg accttgtttc cttcacgaac cagcgcgtcg acgcgaacgc ctgggataaa
     5581 ctgcgcgccc gcctgctcgg cttgttccat caaccacggg tccagacgat tacgcaatac
     5641 ggtataagat gcgtgttgag gaacatctgg ttgctcgcgg tgaaaatcga gggtaacggc
     5701 gctttcttcg gttaagaagg agattttctc gcgtgtgacc ttgcgttcta ccggcgctga
     5761 tactgcaaag cctggaatga ttgcttcaag tgtgtgggca taaagacgcc cgccggtcat
     5821 gtttttacat ccggcactgt cgccgcgttc tatcaccagg acatccagcc cggctcgtgc
     5881 catgaccagt gcggcaacgc taccagcaac gcccgcaccg accacaatgg catcaaattt
     5941 gtcatccgac atgcgttatg ctcccttcct gctgcgttta cagcgggaga tgtggttgaa
     6001 aggggaatta gcggctcaac tggctaatca gggcagggac gactttgtag atatcgccca
     6061 ccagaccgta gtcggcatag ttgaagattg gcgcattttt atctttattg atggcgacaa
     6121 tcacttttac gccgttgccg ccaaccatat gttggatctg cccggagatc cccagcgtca
     6181 ggtagagatc ggatttcagc aacacgccgg agacaccgat ataacgttca cgctccatcc
     6241 agttctcgcc ttcggcaatt ggacgtgaac agccgacttc agcattcagc accgccgcca
     6301 gttcgtggac cattttcagg tcatcctgcg ccgccagacc acgaccgacg ccaaccacac
     6361 gttttgcttt gctcaggtcc acgctgcttg cggctttcgc acggcgttcg cgacagagaa
     6421 tttcatgacg cggagcaaca taaggtaccg tttctgtcgg gcactgatga gaggtatcac
     6481 tggtgcacgg ttcctgaaca ccgggtgcaa gggtaatgat cgccagcggg ctgttgatct
     6541 tttcctgagc gaacgctaac ccgccataca tccggtgttc ggcgcaaatg tgaccatcga
     6601 caatatccac cgccgtggca tcgttcacca gcgccgcatt cagttgcaca cttaaccgtg
     6661 ctgccagagc tttaccacgt ttcgtcgcgg ccaacagcaa catagcgggg tgtttatctt
     6721 tcagcagggc agcaatgctt tcggcgtaat tttcagtgcg ttgcagcgcg tcgttttgct
     6781 caagaacata aatacatttt ggaccataag gcataactgc ctgcgcctgg tcggtatttt
     6841 gtacaatggc atacacctgt tggccccatt gctgagcgcc gccaaacagt tcagcataac
     6901 gttcaggatt atcgctaaag acccagacgc tgtttaattg actcatcgtt tcttctccgt
     6961 aggcttcagt tcagggcttt cttcagatgt tccgccagct cggcaatggc ctccggcgaa
     7021 tcgttatcga gaatgatgtg cttacgttct gtttgcggcg gtacgcgaat gccgaccagt
     7081 tcagcaagtg gcgcgctctg gctccagtca atatcacttg cctgccactg atttaccggt
     7141 tttttacccg cgccgagaat ggctttcatc gaaggaatgc gtggcacgtt aatatcggag
     7201 gtgacgcaga gcacggctgg aacagagagt tcaataactt caacatcatc ttcaagcgtg
     7261 cgttcaatca ccagtgtatt gccctgacgc tgaatagcac tcactgcatt aatcaccgga
     7321 agttgcagaa tttctccgac cagcaagcca acctgctggg cataaaggtc gccggaacct
     7381 tcaccaaaga tcagtaaatc gaagccgatc ttttcaattg ctgccgccag cgcttttgcg
     7441 gtatcgagag gcagtgcatg ttcaagttgc gcatcctgca ccaaatacag gctgtgcggc
     7501 ccgcgggata gcacgtcttt gcgcactttc gagttctgca acaatgagcc gccaacggtc
     7561 agcgcggcta tctcatcgtc atctgttgcg agctggcttg cagcttcaat ggcattgaga
     7621 tcgaactggc tgattttggc gtcggcattg tcgaaattca gggtgtattc tggagtgaca
     7681 acaatgtcct gttcttcagg caccagctta aagcaggtta ttattttcat gatttctcct
     7741 gtgagtcgca tacggaacca tctccgatgg aaggtaaaaa tggcctgaaa atagtgtgcg
     7801 atagcggtgc gggggggggg gcgacggagc aagctgtttt ccttgccaac aatcttgcaa
     7861 aaaaaatgcg attgctaaaa caaaagtgca atttgcggca atatagttat tccatgaccg
     7921 tacaatcttg cattcagtgt aaataatctt gtttagctct cgctaaacgc ggttgtataa
     7981 ccgcaataca cgccgtattt tatccggcat atattgtttt tgcttgctca tggaataggg
     8041 aggttgtcat gtatcaacgc tgttttgata atgccagtga aacgctgttt gtcgccggta
     8101 aaacgccacg gctttcacgt tttgcattta gcgatgatcc aaaatgggag tctggacatc
     8161 acgttcatga caatgaaacc gagctgattt acgtcaagaa aggggttgca aggtttacca
     8221 tcgattcttc gttatatgtc gcacatgcgg atgacattgt ggtgatagaa cgcggcaggc
     8281 tgcatgcggt ggcctctgac gttaacgatc cggcaacgac gtgtacctgt gcgctgtacg
     8341 gctttcagtt tcagggggct gaggaaaatc agttactgca accgcattct tgtccggtaa
     8401 ttgccgcggg gcagggaaaa gaagtcataa aaaccttatt taatgagcta agtgtgattt
     8461 tgccgcaaag taaaaatagc caaacatctt cgttatggga tgcattagcc tatacattag
     8521 c
//
LOCUS       DAZOYF010000170         8501 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZOYF010000170 DAZOYF010000000
VERSION     DAZOYF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.171"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..104)
                     /locus_tag="ACHUOF_003536"
     CDS             complement(<1..104)
                     /locus_tag="ACHUOF_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0612566.1"
                     /translation="MSRKTRRYSKEFKAEAVRTVLENQLSISEDASRL"
     gene            149..328
                     /locus_tag="ACHUOF_003537"
                     /pseudo
     CDS             149..328
                     /locus_tag="ACHUOF_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(337..735)
                     /locus_tag="ACHUOF_003538"
     CDS             complement(337..735)
                     /locus_tag="ACHUOF_003538"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR0612567.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(735..962)
                     /gene="vapB"
                     /locus_tag="ACHUOF_003539"
     CDS             complement(735..962)
                     /gene="vapB"
                     /locus_tag="ACHUOF_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR0612568.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            1044..6297
                     /gene="traI"
                     /locus_tag="ACHUOF_003540"
                     /pseudo
     CDS             1044..6297
                     /gene="traI"
                     /locus_tag="ACHUOF_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6317..7063
                     /gene="traX"
                     /locus_tag="ACHUOF_003541"
     CDS             6317..7063
                     /gene="traX"
                     /locus_tag="ACHUOF_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR0612569.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7118..7678
                     /locus_tag="ACHUOF_003542"
     CDS             7118..7678
                     /locus_tag="ACHUOF_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR0612570.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7809..8021
                     /locus_tag="ACHUOF_003543"
     CDS             7809..8021
                     /locus_tag="ACHUOF_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR0612571.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8265..>8501
                     /locus_tag="ACHUOF_003544"
     CDS             8265..>8501
                     /locus_tag="ACHUOF_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR0612572.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1953 a         2123 c         2706 g         1719 t
ORIGIN      
        1 aataatcggg aagcgtcttc actgatcgaa agttgatttt caagaaccgt tctgacagct
       61 tcggctttga actctttaga gtaacgtcgg gtttttctgc tcattattag ctccttctga
      121 tgccattcta tttcaggaag gagtgtccgt taaactcagg ctacctcaga tcagtgaatc
      181 cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc atccggacac
      241 ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg aacgcgggga
      301 ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt cagttctcag
      361 gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc cccgactgcg
      421 ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac gggcaagttc
      481 tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa catcaatgcg
      541 ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt tttctgcacc
      601 atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt tcaggttaaa
      661 acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt tggtatcgag
      721 cataaacttc agcatcagaa tgactccctt tcttgcatgc cgggctgttc cctgttatcc
      781 ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc tccggctggc
      841 gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt ttccggcaat
      901 gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac ggtggtttcc
      961 attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat tgataccggg
     1021 atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg gccggaagtg
     1081 ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg ggagaacgct
     1141 gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag gatgtcttta
     1201 cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg caggatggca
     1261 gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt atctccatga
     1321 tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc gtggattttg
     1381 ctgtccgtca ggtggaggcc agtgcctcca cctgacggac ggacagtcag aaacggtgct
     1441 gaccggtaat ctggtgatgg cactgtttaa ccacgacacc agtcgcgatc aggaaccaca
     1501 gttacacacg catgcggtgg tgactaatgt tacgcagtat aacggcgaat ggaagacact
     1561 gagcagtgac aaagtgggga aaacggggtt cattgagaat gtgtacgcta atcagattgc
     1621 ctttggcagg ctctaccggg aaaaactgaa agagcaggtt gaggcgctgg gctatgaaac
     1681 tgaagtggtc ggtaagcacg gtatgtggga aatgccgggt gtaccggtgg aggccttttc
     1741 cggacgctca cagactatcc gggaggccgt cggggaagac gcctcgctga aatcccggga
     1801 tgtggcggcg ctggatacgc gtaaatccaa acagcacgtc gatcctgaag tcagaatggc
     1861 cgagtggatg cagacgctga aggaaaccgg gttcgacatc cgggcatatc gtgacgcagc
     1921 ggaacaacgg gcatataccc gcacgcagac acccggaccc gcttcacagg acgggccgga
     1981 tgtgcagcag gcggtgacac aggccattgc cggattaagt gaacgcaaag tgcagtttat
     2041 gtatacggac ctgctggcca ggacggtcgg catactgccg ccggaaaatg gtgtgattga
     2101 acgggcacgc gccggtatcg atgaggccat cagccgtgag cagcttatcc ccctcgatcg
     2161 tgagaagggg ctgttcacgt tcgggattca tatgctcgat gagctgtcag tccgggcact
     2221 cagtcgtgac atcatgaaac agaaccgggt gaccgtacat ccggagaaaa gtgtcccccg
     2281 gacggccggt tacagcgatg ccgtcagtgt tctggcacag gaccgtccgt cgctggccat
     2341 tgtgtccggg cagggcggtg cagccgggca gcgtgagcgg gtggctgaac tggtcatgat
     2401 ggcccgggag caggggcggg aggtgcagat tatcgctgct gaccgtcgct cgcagatgaa
     2461 cctgaagcag gatgaacggc tgtccggtga actgataacc ggacgtcgtc agttgctgga
     2521 aggaatggcc ttcacgccgg gcagtaccgt tatcgtcgac cagggcgaaa aactctccct
     2581 gaaagagacg ttaaccctgc tggatggtgc cgcacgtcat aacgtacagg tcctgataac
     2641 cgacagcggg cagcgaaccg gtacaggcag tgcactgatg gccatgaagg atgccggggt
     2701 gaacacatat cgctggcagg ggggagaaca gcgaccggcc accatcatca gtgaaccgga
     2761 ccgtaatgtc ggctggccgg agattttgcg gccagcgtga aagccggaga agagagcgtg
     2821 gcacaggtca gcggagtacg ggaacaggcc atactgacac aggccattcg cagtgagctg
     2881 aaaacacagg gcgtgctcgg acacccggag gtaaccatga ccgccctgtc accggtctgg
     2941 ctggacagcc ggagccgtta tctgcgggat atgtaccgcc cgggaatggt gatggagcag
     3001 tggaacccgg agacacgcag tcatgaccgc tatgtgacag aacgtgtgac ggcgcagagt
     3061 cacagcctga ccctgcggaa tgcgcagggc gaaacgcagg tggtgcgtat ttcttccctg
     3121 gacagcagct ggtcgctgtt ccggccggaa aaaatgccgg tggcagacgg tgagcgtctg
     3181 agggtgacag ggaaaattcc cggactccgc gtctccggcg gtgaccgcct gcaggtggca
     3241 tccgtcagtg aagatgcgat gacggttgtt gtgccgggac gggctgaacc ggccaccctg
     3301 cctgtgagcg attcaccgtt cactgcactg aagctggaga acggctgggt ggaaacgccc
     3361 gggcattccg tcagcgacag tgcgacggtt tttgcctccg tcacacagat ggcaatggac
     3421 aatgccaccc tgaatggtct ggcccgcagt ggtcgtgatg tccggctgta ttcctcactg
     3481 gatgaaaccc gtactgcgga aaaacttgcc cgccatccct cctttacggt ggtttctgag
     3541 cagataaagg cgcgtgccgg tgagacattg ctggaaaccg ctatcagtct gcagaaagcc
     3601 gggcttcaca cgccggcaca gcaggccatt catctggccc ttcctgtgct ggaaagtaaa
     3661 aacctggcct tcagcatggt ggacctgctg acagaggcga agtcgtttgc tgcagaagga
     3721 accggttttg ctgacctggg aggggaaatc aatgcgcaga taaaacgggg tgatttactg
     3781 tatgtggatg tggcaaaagg ctatggcaca ggcctgctgg tttcccgtgc gtcgtatgag
     3841 gcagaaaaga gcattcttcg ccatattctc gaaggtaagg aggcggtcac gccgctgatg
     3901 gagagagtac ctggcgaact catggagaaa ctgacatcag gacagcgcgc cgccacccgt
     3961 atgatactgg aaacgtctga ccgtttcacg gtggtgcagg gctatgccgg tgtgggtaag
     4021 accacacagt tccgggcggt gatgtcagcc gtgaacatgc tgccggagag tgaacgtccc
     4081 cgtgtcgtgg ggctggtgcc cacacaccgt gcggtcgggg agatgcgcag cgccggcgtg
     4141 gatgcacaga cactggcgtc ctttctgcat gacacgcagc tgcagcagcg cagcggagaa
     4201 actccggatt tcagcaacac gctgttcctt ctcgatgaga gctctatggt gggcaatacc
     4261 gacatggcac gggcatacgc cctgattgcg gccggtggcg gtcgggctgt ggccagcggt
     4321 gacacggacc agctgcaggc catcgcgccc ggtcagcctt tccgtctcca gcagacgcgc
     4381 agtgctgccg atgtggtcat catgaaggag attgtgcgtc agacgccgga actgcgggag
     4441 gcggtataca gcctgattaa ccgggatgtg gaaagggcgc tgtccgggct tgagcgtgtg
     4501 aaaccgtctc aggtgccacg tctggagggc gcatgggcac cggagcactc cgtgacggag
     4561 ttcagtcaca gccaggaagc gaaactggca gaagcgcagc agaaggcgat gctgaaaggc
     4621 gaggcttttc cggatgtccc catgacactg tatgaagcca ttgtccgcga ctataccggc
     4681 aggacaccgg aagcacggga gcagacgctg attgtcacgc acctgaatga ggaccggcgc
     4741 gtactgaaca gcatgattca tgatgcacgg gaaaaggccg gagaactggg gaaggtgcag
     4801 gtcatggtgc ctgtcctgaa cacagcgaat atacgcgacg gggagctgcg tcgtctctcc
     4861 acctgggaga ataacccgga tgcccttgcc ctggtggata atgtgtatca ccggattgcc
     4921 ggtatcagta aggatgacgg gctgataacc ctgcaggatg cggaaggtaa cacgcggctg
     4981 atttcgcccc gggaggcggt ggctgaaggc gtcacgctgt acaccccgga caccatccgg
     5041 gtggggaccg gtgacaggat acgcttcacg aagagtgacc gggagcgcgg ttatgtggcc
     5101 aacagcgtct ggacggtgac agcggtttcc ggtgacagtg tcacactttc tgacggtcag
     5161 cagacccgtg tgattcgccc cggtcaggag cgggcagagc aacatattga cctggcctat
     5221 gccatcaccg cccacggtgc acagggggcg agtgaaacct ttgccatcgc gcttgaaggc
     5281 acggaaggta accggaaact gatggccggc tttgagtcag cctacgtggc cctgtcgcgt
     5341 atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga tgccatcaac
     5401 aatgccgtac agaaaggaac tgcccacgat gtgtttgagc cgaaaccgga ccgggaggtc
     5461 atgaatgcag agcggctgtt cagtacggcg cgggagctgc gggacgtggc ggcaggccgt
     5521 gccgttctcc gtcaggcggg gctggccggg ggagacagtc ctgcacggtt tattgctccg
     5581 ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt ttgaccgtaa cggcaagtcc
     5641 gccggtatct ggctgaaccc gctgaccacg gatgacggaa acgggctgcg gggattcagc
     5701 ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg tggccctgca gggcagccgt
     5761 aacggagaaa gcctgctggc tgataatatg caggatggcg tccggattgc ccgtgataat
     5821 cctgacagtg gtgtggtggt aagaatcgcc ggtgaaggcc gtccgtggaa tcccggtgcc
     5881 atcaccggtg gccgcgtgtg gggggatatc ccggacaaca gcgtccagcc gggagccgga
     5941 aatggtgaac cggtcacggc agaggtactg gcacagcggc aggctgaaga ggccatccgc
     6001 cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa tggcagagaa caaacctgac
     6061 ctgccggacg gcaaaacaga gcaggctgtc agggaaattg ccgggcagga gcgtgaccgg
     6121 gctgccataa ctgaacggga agccgcgctg ccggagagtg tactgcgtga accacaacgg
     6181 gtgcgggaag cggtccggga agttgcccgg gaaaatttgc tgcaggagcg tctgcagcag
     6241 atggagcggg atatggttcg tgacctgcag aaagagaaaa ccccgggcgg agactgatac
     6301 aggaagacaa acactgatga caacggataa cacgaacacg acacgtaacg attcactggc
     6361 tgcacggacc gatacctggt tgcagtcatt tctggtctgg tcacccggac agcgggatat
     6421 catcaaaacg gtggcactgg tgctgatggt actggaccac atcaatctga tattccagct
     6481 gaagcaggaa tggatgtttc tggccgggcg aggagccttt ccgctttttg ccctggtgtg
     6541 ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg gccattaacc ggctgtgggg
     6601 atggggaatt attgcccagt tcgcatatta tctggcaggc tttccttggt atgaggggaa
     6661 tatcctgttt gcctttgcag tggcggcaca ggtgctgacg tggtgtgaaa cgcgcagtgg
     6721 gtggcgtaca gcagcggcca tactcctgat ggccctgtgg gggcctttgt ccggcaccag
     6781 ttacggtatt gccgggttgc tgatgctggc agtcagcaac cggctgtacc gggcggaaga
     6841 cagagcggaa cgtctggcgc tggtcgcctg cctgctggct gttattccgg cgcttaacct
     6901 tgccaccagt gatgcagcgg cggtggccgg tcttgtgatg acggtgctga ccgttggtct
     6961 ggtgttgtgt gccggaaaat cattaccccg tttctggccc ggagactttt tcccgacgtt
     7021 ctatgcctgt catctggctg tgctgggcgt tctggcgctg tgacgggtgt ggtatctttg
     7081 gccgcaagag gatgattcgt cagaggcaga acacagcatg acagagcaga agcgaccggt
     7141 actgacactg aagcggaaga cagagggaac agcgcctgtc cgcagccgga aaaccatcat
     7201 caatgtcacc acgccaccaa agtggaaggt gaaaaagcag aagctggccg agaaagcggc
     7261 ccgtgaagca gagctggcgg caaaaaaagc gcaggccaga caggcgctgt ccatttatct
     7321 gaacctgccc acgctggatg aggctgtgaa taccctgaag ccctggtggc cgggattatt
     7381 tgacggtgac acgccccggc ttctggcctg cggtatccgg gacgtgttac tggaagacgt
     7441 ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc agggcgctga aggccatcac
     7501 ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg acacggaagg
     7561 gtatgtgacg gagcatattt ctcaggagga ggaagcgtat gcgggagcgc gtctggcgaa
     7621 aattcgtcgc cagaaccgga taaaggcaga acttcaggcc gtgcttgatg agaaataaaa
     7681 aaagcctccc ccagagagag gaggcaggga aataaggctt aaaaggaaat aacttccgct
     7741 tccgaaagga agccagtcca ctgaattatt tatcacaata atactgacaa ctaaaggggg
     7801 acaagaaaat gaatggattc agaaacagtt cacggaacgg ccaggtctgg cgttaccagc
     7861 gtgccggcgg gcgggccgtt attctggaag tcagtggacg ctggatggaa gcggcagaag
     7921 catggcgacg ggctgcctgt gttgcgccta ggacagactg gcagcagttt gcccggaaaa
     7981 gggctgaaca ctgccaccgg cgctgccggg gaagggtgta acgaaaaaag cagccttcat
     8041 tactctagga gtcagaaggc tgaaaggagt ttatgagatt tgcttcttaa tatccggtcg
     8101 ggcagtatga ccggacagtt ctgagtagca cagagtcttt tccgcattaa gtcgattttt
     8161 tatattttgt gattagtttc acaaaataag gtgttattca atgtgtgctg cactattcag
     8221 gggggctgaa atacaggtgg catttatctg atatggagaa taacatgagg aaatatattc
     8281 cactggcatt atttatcttt tcatggccgg tattgtctgc agatattcat ggacgggttg
     8341 ttcgtgtact ggacggtgat accatcgagg tcatggactc actgaaggcg gtgcggatca
     8401 ggctggtaaa tattgatgca ccggaaaaga aacaggatta tggccggtgg tcaacagaca
     8461 tgatgaaatc actggtcgca ggaaaaacgg ttaccgtcac a
//
LOCUS       DAZOYF010000171         8495 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZOYF010000171 DAZOYF010000000
VERSION     DAZOYF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.172"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            44..397
                     /locus_tag="ACHUOF_003545"
     CDS             44..397
                     /locus_tag="ACHUOF_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR0612573.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..2512)
                     /gene="flhA"
                     /locus_tag="ACHUOF_003546"
     CDS             complement(434..2512)
                     /gene="flhA"
                     /locus_tag="ACHUOF_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFR0612574.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="ACHUOF_003547"
     CDS             complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="ACHUOF_003547"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR0612575.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="ACHUOF_003548"
     CDS             complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="ACHUOF_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR0612576.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="ACHUOF_003549"
     CDS             complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="ACHUOF_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR0612577.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="ACHUOF_003550"
     CDS             complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="ACHUOF_003550"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR0612578.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="ACHUOF_003551"
     CDS             complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="ACHUOF_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0612579.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6415..6876
                     /gene="hycA"
                     /locus_tag="ACHUOF_003552"
     CDS             6415..6876
                     /gene="hycA"
                     /locus_tag="ACHUOF_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR0612580.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7001..7612
                     /gene="hycB"
                     /locus_tag="ACHUOF_003553"
     CDS             7001..7612
                     /gene="hycB"
                     /locus_tag="ACHUOF_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR0612581.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7609..8495
                     /locus_tag="ACHUOF_003554"
                     /pseudo
     CDS             7609..8495
                     /locus_tag="ACHUOF_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
BASE COUNT         1904 a         2307 c         2287 g         1997 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc agttcgcgta
      721 cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga
      781 tattgcgccc cagacgacgg gcaattttga aggtaaaggc tttcgccagc agcggaatat
      841 cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa tacgttcagg cggtaataga
      901 gatcgctacg gaactctcgg tcggcgacca tttttttcag atcgcggtta gtcgcggcaa
      961 ttaaacgtac gtccgtctga atgattttgt tgctgccgag acgttcaaac tcctgttcct
     1021 gcaatacacg cagcaacttc ggctgtaact ccagtggcat atcgcccact tcgtcgagga
     1081 acagagagct tttatccgcc agttcaaaac gaccaatgcg ctgggcgttg gcaccggtaa
     1141 aagccccacg ctcatgacca aacagatcgc tttccagcaa tccggcaggc atcgccgcgc
     1201 agttcatttt gaccatgcgg cgattattac gcccactgag attatggatc gcacgggcaa
     1261 tcagctcttt acccgagcca gtttcaccga ggatcagcac ggtactgtca ctttgcgcca
     1321 ccatttcaac ttgtttaagc acgctgtaca tggcttcgct gcggccaata atctcgccaa
     1381 attcactatc aacattgttg agctgctcgg tcagggcgag gttttcatca accagccgtt
     1441 ctttcagacg atggatttcc tgataggcga gggcgttatc gacagcgatt gccacacgtt
     1501 cggcaatctg gcgcagcaaa ttcagattgg tagtggtaaa cactttctct tcgcattgcg
     1561 ccagtttcag cacgcccagc atggtgtcgc cagacatcag cggtaacagg cacaaggttt
     1621 gaatctggtt gccccaggtg tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct
     1681 cgtagagatt gatcagcagc atctctttac ttttgaacac gcgttcggtg agggttccgg
     1741 cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt atcaagatag tgagtggagt
     1801 agatgttgag tttgttttta cggtggctgc gtaagacaat gctgatatcg tcaatgtcga
     1861 aatagtaatg gatttctttg gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa
     1921 gcaccgcgtt ggtaatggca accaggatgc ggaagttatc gcgctcccgg cataacaact
     1981 catagtcgac attgttgacg acgcggctct ggatttgttc ggtgacgaca gaaacgatct
     2041 gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca gggacgctcg tcataacgaa
     2101 taaattcaca gccaccaaaa atatgccctc ccgccgccag tggcatcagg caatagtgac
     2161 caaatttggg gtatagccca cctgcggcca gctgcggcca ggtttcacaa aattcttcgt
     2221 aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa
     2281 cagtttcgtc ttcatattta atgggggtgt ctttttcacg cgacgcgtaa taagacgcac
     2341 gttgagtctg cgcttgccac aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta
     2401 ccagttgcga aagagcctca cacagcgagg ccagatcggg ctgctgcaat agtgtccgag
     2461 tgatgtcgaa caacccttgc tgtccgagat cactcatcgg tgtatatgac attttgttct
     2521 tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac taactatagg agatttagaa
     2581 ttttattagc atatacgcgg aagcggttcg gcgtgtggta aatcgagggt tcgtttcacg
     2641 ccatacagac cggcaagacg aacaccttta cgttccacca cttcaccaat cagcgccgcg
     2701 tctttcccca gtggatggga atgtaacgct gccagcactt gctctgccgc gttgcgttca
     2761 acggcgatca ccagtttgcc ttcgttggca aagttcaggg cgtccagtcc cagcaattcg
     2821 caaacgccgc gcacggcagg tttaaccggc agcgctgatt cagaaatttc aataccacaa
     2881 ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac caccacgggt ggcatcacgc
     2941 agcgctttca cgccgggaat gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag
     3001 tcgctgacca gttcgccatc cagccccagc tgctcacgca ggttaaggat agtcgccccg
     3061 tggtcgccga gtgtaccgct aaccagcaat acatcgcctg cggttagcgt ctgtgcgccc
     3121 cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt tgataaacag tttatctgcc
     3181 gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg
     3241 gtttctgcca tgctggtcac tacggctttc agtgtctcca tcggcaatcc ttcttcgagg
     3301 ataaagccac aggagagata gcgcggaata gcgccactgc ccgcaacgtc attcgcggtg
     3361 ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga acagcgggtc aatgacgtaa
     3421 ctgtcggtgg agaacgccag acggtcgcct tccgctacca gctgcgccag atcaagacgt
     3481 gcctgatctt cctgctctgc cagccacggg ttggcaaagg cttccataaa caggctgttg
     3541 attaattgct gcatcgcctg gccgccgctg ccgtgggcga gttggatatt attcacgctt
     3601 cactctcctg ctgacgatac tgataccacg cggcgcacgc tccttcggag gagaccatca
     3661 gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa cagcgggcat tgatgcggct
     3721 tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg
     3781 gtgccgggcg gaaatgtgct tcggcatcga atcgttgata atccggcgtc aggtgcacgc
     3841 cagaagattc aatcacgcct aagccgcgcc attcgctgtc gccgttgaca cagaacacat
     3901 cggcaatcgc ctgttgcgcc agtaggttac cggcatccgg taccacccga cgatactgat
     3961 tctctacctt gctgtgggcc gctattttct gctccaccag catgaccacg ccttgcagta
     4021 gatcaagggg ttcgaaacca gcgaccacca gcggacgatg aaaatcgctg gcgataaaat
     4081 tataggcatc agtgccgata accatactaa cgtggcccgg cgcgaggaac gcgtcgatac
     4141 cgttatccgg ctgttccagc aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga
     4201 agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg
     4261 gcatggtggt ttcaaaacct aagccgaaga acaccacttt gcgggttgga ttctcctgcg
     4321 ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg
     4381 cctgcaacag cgatccctgt ttccctggta cgcgcatggc gtcgccaaag gtacagaaga
     4441 tgacttccgg atggctggca atctccacgc aggtgtcgat tctacccatc ggcagtacgc
     4501 acaccgggca acccggaccg tggataaact caacgttttc aggcagtaac tggtcgaggc
     4561 cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc cataatccgc agagggcgtt
     4621 cggcggtgta agagagatgt gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct
     4681 gttccggcgc gcgatattca tcaacaaaac gcattatttt tcctcgccat acaacagcgc
     4741 gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc
     4801 ggcttcatta attacgctca tggcaaagcc aacgtgaacc agtacccact ggcccacgcg
     4861 cggctgaccg ttttcatcgc agctgccgac taacgttaaa tcgacatcgc gctgaatgcc
     4921 gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg atctggccgg gaacgcctat
     4981 gcacatcgct gtgtctccag ccagttcagc cactggtcca tcccttcgcc gctggtggcg
     5041 gaaataagga tgatttcaat ttctggattg acttcgcggg cgcaggcgat gcacttctca
     5101 acgtcaaagt tgagatacgg caacaggtca actttgttga gcagcatcag cgaggcggcg
     5161 gcaaacatat gcggatattt cagcggtttg tcttcacctt cggtaacgga aagcaccgct
     5221 actttgtgtt tttcaccgag atcgaagctg gccgggcata cgaggttgcc aacgttttcg
     5281 ataaacagaa taccgttatc gtccagtggc agacgcggtg cggcgtcggc aatcatctgt
     5341 gcgtcaagat ggcagccttt accggtgttc acctgaatcg ctggtgtacc ggtagcgcga
     5401 atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa taaccgcgca cggaacgctg
     5461 tctttcaggc gcattaaggt ttccgtcagc agggtggttt taccggaacc agggctggaa
     5521 accaggttga gcaccagttg cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga
     5581 cggttgtttt tgtccagcac gtcaatttcg acttccagca tccgacgctg gctcatgccc
     5641 ggtgtgtgag tgcccgcttc gccatgaccg taatgcaggt cgccttcttc agtctggctg
     5701 ggggtaaatt caggcgcttt aatgccggtg attttcatct gcgggcgtgc cgccggggca
     5761 aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat caccctcgat atacaggttg
     5821 ccttcaccgc aaccgcatgt tgtacacatc gctcactcct ggtctatttc tatccgccga
     5881 atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac catgacactg tggacagcgg
     5941 cggacgcgct gggtcagtag cgtcacatac tgttggcatg tttcacacca gcattcggcc
     6001 tcttgttctt cgaggtgcag tttacaacct tccgccacgc tgccgcggca aaccagatca
     6061 aaacaaaagg caagagagct ggtttcgaca caagaaaatg cgccaatttt gagccagacc
     6121 ccagttacgc gttttgcgcc gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt
     6181 tggcagaggg ttatttcgtg catatcgcct cccattaact attgccagct acaagcaata
     6241 attgcgccag tgttgattat ccctgcggtg aataatgtcg atgatgtcga aatgacacgt
     6301 cgacacggcg acgaaattca tctttagctt aaaaatctct ttaataacaa taaattaaaa
     6361 gttggcacaa aaaatgctta aagctggcat ctctgttaaa cgagtaacct gacaatgact
     6421 atttgggaaa taagcgagaa agccgattac atcgcacagc ggcatcgtcg cctacaggac
     6481 cagtggcaca tctactgcaa ttcgctggtt caggggatca cgttatcgaa agcgcgcctg
     6541 catcacgcca tgagctgcgc gccggacaaa gaactctgtt tcgtcctttt tgaacatttt
     6601 cgtatttacg tcaccctggc ggatggcttt aacagccaca ccatcgagta ttacgtcgaa
     6661 acaaaagatg gcgaagacaa acagcggatt gcgcaggcgc aactgagcat tgacggcatg
     6721 attgatggca aggtcaacat ccgcgatcgc gaacaggttc tggaacacta tctcgaaaaa
     6781 atcgctggcg tttacgacag cttatacacc gccattgaaa acaatgtgcc ggtgaattta
     6841 agccaactgg taaagggaca aagcccggca gcatgagctg aggctttgcc cgttttgcag
     6901 gcgttacgcc tgtttgggga tgggcgtgtc gatgagtgtc gaaaatgaca tttcatcggc
     6961 atgttttcgt caaaaatgac aatcacctga ggaatgcctg gtgaatcgtt ttgtaattgc
     7021 cgactccacg ctctgtatcg gctgccacac ctgtgaggcc gcctgttcag agacgcatcg
     7081 ccagcacggc ctgcaatcaa tgccgcgcct gagagtgatg ctcaatgaaa aagaatctgc
     7141 gccgcagctc tgtcaccact gtgaagatgc accctgcgcg gtggtctgcc cggttaacgc
     7201 catcacccgc gttgatgggg ccgtgcagtt gaatgaaagt ctgtgcgtaa gctgcaaact
     7261 gtgcggcatc gcctgcccgt ttggcgcaat tgaattttcc ggcagccgtc cgctggatat
     7321 tccggcaaac gccaataccc cgaaagcgcc accggcaccg cctgctccgg cgcgtgtcag
     7381 cacattgctt gactgggtgc caggtattcg cgcgatcgcc gtcaaatgtg acctttgtag
     7441 ctttgatgaa caaggtccgg cctgtgtgcg gatgtgcccg actaaagccc tgcatctggt
     7501 ggataacacc gatatcgccc gcgtcagcaa acgtaagcgt gagctgacct ttaacacgga
     7561 ctttggcgat ctcaccttgt ttcagcaggc tcaaagtgga gaggctaaat gagcgcaatt
     7621 tccctgatca atagcggcgt ggcatggttt gtcgccgccg ctgttctggc atttctcttt
     7681 tcaaaaagcg ctaagtggct ggatagccgg aattggcggc gcggttggta gtctgtatac
     7741 ggcagccgcg ggcttcactg tactgactgg cgcggttggc gtgagcggtg cgctgtcgct
     7801 ggtaagctac gatgtgcaaa tctccccgct taacgcgatt tggctgatta ccctcggcct
     7861 ttgcggtctg tttgtcagcc tctataacat tgactggcat cgccacgcgc aggtgaagtg
     7921 caacggcttg cagatcaata tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa
     7981 cctcggcatg ttcgtggtaa tggccgaagt catggccttg tgtgcggtgt tcctcaccag
     8041 caacagcaaa gagggcaaac tgtggtttgc gctgaggcgt cttggcactc tgctgctggt
     8101 gattgcttgc tggctgctgt ggcagcgtta tggcacgctg gatctgcgcc tgctggatat
     8161 gcgtatgcaa cagctgccgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
     8221 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
     8281 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
     8341 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
     8401 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
     8461 cctgctggct taccacaccc tggaaaatat cggca
//
LOCUS       DAZOYF010000172         8083 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZOYF010000172 DAZOYF010000000
VERSION     DAZOYF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.173"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..97
                     /gene="yodD"
                     /locus_tag="ACHUOF_003555"
     CDS             <1..97
                     /gene="yodD"
                     /locus_tag="ACHUOF_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR0612582.1"
                     /translation="SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            395..1210
                     /locus_tag="ACHUOF_003556"
                     /pseudo
     CDS             395..1210
                     /locus_tag="ACHUOF_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(1207..2901)
                     /gene="dgcQ"
                     /locus_tag="ACHUOF_003557"
                     /pseudo
     CDS             complement(1207..2901)
                     /gene="dgcQ"
                     /locus_tag="ACHUOF_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(3072..3254)
                     /locus_tag="ACHUOF_003558"
     CDS             complement(3072..3254)
                     /locus_tag="ACHUOF_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR0612583.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(3333..4250)
                     /locus_tag="ACHUOF_003559"
     CDS             complement(3333..4250)
                     /locus_tag="ACHUOF_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR0612584.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4423..5343
                     /gene="yedA"
                     /locus_tag="ACHUOF_003560"
     CDS             4423..5343
                     /gene="yedA"
                     /locus_tag="ACHUOF_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFR0612585.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(5332..5802)
                     /gene="vsr"
                     /locus_tag="ACHUOF_003561"
     CDS             complement(5332..5802)
                     /gene="vsr"
                     /locus_tag="ACHUOF_003561"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR0612586.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(5783..7201)
                     /gene="dcm"
                     /locus_tag="ACHUOF_003562"
     CDS             complement(5783..7201)
                     /gene="dcm"
                     /locus_tag="ACHUOF_003562"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR0612587.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(7268..7769)
                     /locus_tag="ACHUOF_003563"
                     /pseudo
     CDS             complement(7268..7769)
                     /locus_tag="ACHUOF_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(7848..>8083)
                     /gene="drpB"
                     /locus_tag="ACHUOF_003564"
     CDS             complement(7848..>8083)
                     /gene="drpB"
                     /locus_tag="ACHUOF_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR0612588.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRSARCRRYE"
BASE COUNT         1929 a         2213 c         1982 g         1959 t
ORIGIN      
        1 tagcgtcgat ggacgtgaat atcatacatg gcgtgaattg gcggatgcct tcgaactgga
       61 tattcatgac ttcagcgtct ctgaagtgaa tcgttgaatg cacaataaaa aaatcccgac
      121 cctgagggtg tcgggatgaa acttgcttaa gcaagaagca cttaaaaaat tcgttacacc
      181 aggaaatctg atgtgttcat caccttatct gcaatttttt tcgctgacaa gaaaatattc
      241 gcgctatgaa tgattaacgc tacttatgaa tagaaatgtg acgcgcgtca ccccttggtg
      301 gctctggtgt caacgggatc gctcccgaaa aaatcatctt tgattatttg tgctgttttg
      361 agaatcatcc tggtaagggg agtactgcgc gaccatgttt tcaattcaac aaccactact
      421 ggtttttagc gatcttgatg gcaccctgct ggacagtcat agttatgact ggcaaccggc
      481 agccccctgg ctcagccgtt tacacgaagc caatattccc gtcattctct gtagcagtaa
      541 aactttagcg gaaatgctgt acttgcaaaa aatgttgggg ctacaaggct taccgctgat
      601 tgcagagaat ggcgcagtga tccagcttgc tgaacaatgg caggatatag acggttttcc
      661 acgcatcatc tcaggtatta gccatggcga aatctgccag gttttaaata cgctacgcga
      721 gaaagaacat tttaaattca cgacttttga tgatgtcgac gatgcaacca ttgccgaatg
      781 gacgggatta agccgtagct aggcggcgct gacgcagctg catgaggcgt cggtaacgct
      841 aatctggcgc gacagtgacg agcgtatggc acaatttatc gctcgtctga acgaactggg
      901 cttgcagttt atgcagggtg cgcgcttctg gcacgtactg gatgcctctg ccggaaaaga
      961 tcaggctgcc aactggatta tcgcgaccta tcaacaattg tcaggcagac gcccaaccac
     1021 acttggcctg ggcgatgggc caaacgatgc gcctttactg gaggtaatgg attacgcggt
     1081 gattgtgaaa gggctaaacc gtgaaggggt acatctgcat gatgaggatc cggcccgcgt
     1141 ctggcgaacg cagcgtgaag gaccggaagg ctggcgtgaa gggctggacc attttttctc
     1201 cgcccgttaa gcgttatcgc tcgcgaatac ccgattgcgc ccggcctgtt ttgcgaggta
     1261 aagccgacgg tcggccaatg actggagttg ttcaaaatca taatcaccgg tttcctcgct
     1321 gctacttacc cccagcgagg cactgatgcg tatcgttgta ctcttggcga tcagcatctc
     1381 tttttcattt aacttcaggc gaatacgttc tgcgacctcc gcagcctgcg tcagattcgc
     1441 gcctggcaga atcacacaaa actcctcacc accgacccgc ccggcaacgt cctgcgcacg
     1501 caaggaactg ctaattaatc cggcagcatg agaaagaaca cggtcgcccg cctgatgacc
     1561 aaagcggtca ttaatcgctt taaaatggtc aagatcgacc tgaatgacag aaaaaggatg
     1621 ttggtgcgtc tgacacaatt tagcgagcgg acgggctttt tcgaacagtg cgccacggtt
     1681 atataaacgc gttaaggtgt cgtgccacgc ctgccactgc aacgagcttt gcagaacata
     1741 catgttgctg accatccggc gaatcacata ccaggagagg agtaacatgg tggtaaagag
     1801 cgcccacagc agggttaatg caatgctgat actgccgaaa tcgccgcgca cgccttcgct
     1861 tagcgtatgg acgcgcgcca gcacaccgtc gaaatggtcc agacgttccc agctaacata
     1921 gcgactgtcc atacgaatgc cgccccgcgt gtcatgttcc attgcctgcg ccagcaaggc
     1981 cagttcacga ggatcaaaaa tattccctgt tggatggtca ggattggaag aggtcaaaaa
     2041 tctcagctta ctgtcataga gctgatactc accatcgagg tttttatcga tggcgtttct
     2101 taaaaattgc tgcatagtac gcacgggaat actcatcccc agcacgccat accagtagtt
     2161 attactgtct accggaacac tgacggtaac ctgcggttca gtattgctgg cgtgttccgg
     2221 ttgcgaagtg aaccagcgta ccgcgcggtg acgattttct cgttgcgaat ggccgataaa
     2281 ccagggttgg gtgacatagc cgtaataacg cgttggtaca ttgcgcgtaa acaaggtcgg
     2341 ctgcgtcgaa acgtaaaatc cagcacgcga gacatacatc gcctgttcaa ccatcgacga
     2401 ggagttgtgc gccagtcgca gcaagtaacc aacttccagt gcagcggtaa tttcattgtc
     2461 gaggctttca ttttcgcgag acaggaaatt cccctcgctg actaatgcat ccgatacgcc
     2521 attgactggc agggtgcgcc gtcggttgag ttcgatttgc caggcgtgct cgtcgcgatg
     2581 ctgttcgaac tcggttacgg cattacgcag agaggtgaaa tccagtggcg ccacgagagc
     2641 ttcgcgcatg ccattacgca aaaagatcag tttgtcgacg ttatactgca aatgcttatc
     2701 gagcgcgttg gctacgtttt ccagatgatt acgctagctg gagatatagg catcttccag
     2761 caccaccact tcacgccagg tgagcaaggt ggaaaaaagc aataccacga taaagcagag
     2821 attaacgaca tgaccgggcc ccaggcggcg tgcgagtttt ttcaaccagc tctggttttc
     2881 catttttgtc tcgtgctgca ccctgacgac tcccaacctg cttttttatg attctggcat
     2941 aacccggcaa tagcgtaccg caaaacagcg ataattttat aatggtggca tgataagacg
     3001 cgactatcat tcattgcagg cattgggaca ccaggccgga tgtgatccgg cctggtgtta
     3061 atgataccct ttcaaacttc ttccgcagaa cgactcccct cccccggaac aagatcggtt
     3121 tcatgaaaag cttcccgctt gaccccgtaa ccgtcatacc aacgacactc aaccataccg
     3181 ctggagtatc cagtgacaat catccgcggg ccgccctctt taaccgtaac ttcctcacta
     3241 accataaagc tcatactcgc ctcctttttt cgagtgaaac ttgttcacct tagttgaaga
     3301 tggcgaattt tgcataatag ccagtgcgag tattaatgtg cctgaccgcg cattttcgct
     3361 actgctttca ctcccagcac cacgatgccg ccgatgataa atccaagaat cagatttaaa
     3421 acagtcggta atatcatcgc caccactgca ctttgctgcc cggcgatatg ttcaatagcg
     3481 tgatgcagcg gcgcaatacc atgtaccaca atcccgccgc cgacgaggaa catcgccagc
     3541 gtgccgacaa tcgataatgc tttcatcagc caaggcgcga taatcaataa ccctttacct
     3601 aatgcctgca tcagcgcgct agatttttcc gccagccaat accctaggtc atcaatctta
     3661 acgataaccc ctaccagacc gtagacgccc acggtcacta ccagcgcgat gcctgaaagc
     3721 accagcacct gattaagcaa cggcgcctct gccacaatcc ccagcgtgat ggcgacgatt
     3781 tccgcagaca aaataaaatc ggtacgaatc gcccctttaa ttttgtcctt ttcaaacttc
     3841 agcggatcct gcgccgccag cttctccaga cgctgctggc tctgcgctgg atcttcttta
     3901 tgtttacgcg cctccagcat atgcagcact ttctctaccc cttcaaagca gagaaacgcg
     3961 ccaccaatca tcaacagtgg cgtaatcgcc cacgggataa acgcactgat gatcagcgcc
     4021 agcggcacca gaatcacttt attgatcagc gatcctttcg ccacgcccca gaccacggga
     4081 agttcccggt tggcccgcac gcctgaaact tgttgcgcat tgagcgataa atcatcccct
     4141 aatacaccgg cggttttctt cgccgccagt ttgcccatca cagagatatc gtccagcagt
     4201 gtggcgatat catcgagcaa cgtcagtaaa ctactcccgg ccaaaagcat tccctctcat
     4261 ttttattgtt gaagggtgta agtatgaagc aaaaacggca gctctgatac ttgcttcaca
     4321 ggtcaacatt tatttaacaa tcaagtgcaa ttatatctct cgccagcaga atgattttca
     4381 cgtttactat gaaccacctt tttatttccg ccgtgaggga ttatgcgttt ccgccagttg
     4441 ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt ggggctcaac ctattttgtc
     4501 attcggattg gcgtgggaag ctggcctccg ttaatgatgg cgggcgttcg attccaggca
     4561 gccggtattt tattgctggc atttttgcta ctgcgcggac acaaacttcc cccgctacgt
     4621 ccgctgctca atgccgcgct gattggcctg ttattgctgg ctgtcggtaa tggcatggtg
     4681 acggttgccg aacatcaaaa tgttccttcc ggcatcgccg ccgtagtgat tgcaaccgtg
     4741 cccctcttta ccctgtgctt cagtcgcctg tttggcatta aaacgcgcaa actggaatgg
     4801 gtgggtattg ccattgggct tgccggaatc atcatgctca atagcggtgg aaatttaagc
     4861 ggcaatccgt ggggcgcgat tctgatttta atcggctcga ttagctgggc gtttggctca
     4921 gtttatggct cgcgcattac cttacctgta gggatgatgg cgggtgcgat tgagatgctg
     4981 gcggcaggcg tggtgttaat gatcgcatcg atgattgcgg gtgaaaaact gacggcgctc
     5041 ccttccctgt caggcttcct tgcggtcggc tatctggcgc tgtttggttc gattatcgcc
     5101 attaacgctt atatgtattt aatccgtaat gtcagtccgg ctctcgccac cagctacgcc
     5161 tacgttaacc cggtggtcgc ggtcttgctg ggtacgggac tgggtggcga aacgctgtcg
     5221 aagattgaat ggctggcgct cggcgtaatt gttttcgcgg tggtactggt cacgttggga
     5281 aaatatctct tcccggccaa acccgttgtc gcgccagtta ttcaggatgc atcaagcgag
     5341 taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct tcgccgcaga tccactcttc
     5401 cagacgctcg gtaagcgccg catccgtcag cttctcgcgc ctacgtaacg cacactccca
     5461 gacaatcagt acgcgccagc cgagctcctg caagcgactg atatcgcggc gatcgcgttc
     5521 aacattttta cctatcttct ccagccagaa ctcagttcga gtcgcaggca ctttaaacag
     5581 atagcagtga tgatgatgcc agaagcagcc gtgggtaaat atcacgcagc gatattcatc
     5641 aacgacaaaa tccgggcgtc cgggcagact ggcgtcctga acgcgaaacg caaggccctg
     5701 cccggttaac aaactggcga ggcgcttctc aatcgccgta tcacgcgtgg caatcgcgcg
     5761 catatttttg ctgcgggtgg ccttatcgtg aacgtcgacc atgttgtgcc tcttgctgac
     5821 gcaacgccac cgcctgtttg atttttggct caagcagttt tgccaccgcg gcaaagaccg
     5881 gcacgaccac cgagttgccg aactggcgat aggcctgagt gtccgaaacc ggaatgcgga
     5941 atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc gcattcacga ggcgttaacc
     6001 gacgtggacg atgttgctga ttcagcggat cgtcaaagtc tttctcaccc gtggccatat
     6061 cccaaccgcg atcgattaaa atttccgcgc catctttgta ataacgagca gacagcgtac
     6121 gggtgacgct ttgcggattg ttcggataaa ccattccata accgaagccg ttaccgcgcg
     6181 cctgatgttt tttcgcatat cgatagaggt acttccacag caccggcgtc aggatatatt
     6241 tcgcctcaac catcgggtcc agcagctgcg ccagcgtcac tcgctgtgca gggaaacatt
     6301 cgctgatatc acgcagagta aaatcggctt tcagattcag atcgcgacga aaacccacca
     6361 gcacgatgcg ttcacggtgc tgcggcagga aatgtttacc atcgatgatt ttcggatcgt
     6421 ccggcccgtt atcttctgca tcggcaacgt catagcccag ttcgtccagc gtctgcatga
     6481 tgatgcggaa cgtttttccc ttatcgtgac tcttcaggtt tttgacgttt tcgagcacaa
     6541 acatcgccgg acgacgcgcg tcgataatgc gcaccacatc aaagaacagc gtgccctggg
     6601 tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt tttcgatacg ccagccagcg
     6661 aaaatggctg acaagggaaa ccggccagta aaacatcgtg ttcaggaatg tgttgacgaa
     6721 tatgttccgc cgccgcctca tcactcacgc cttcttgatg actgagggtg atgtcgcgga
     6781 tatcttcatt aaaatgatgc gtcgccgggt cgcaataatg gttggcttta taagtgcgta
     6841 ccgcatgttt gttccattcg ctggtaaaca cgcactgtcc accaatcgat tcaaaaccac
     6901 gacggatgcc gccaattccg gcaaatagat cgataaagcg aaacgcataa tgcggatgat
     6961 gttccggtgg tttgggtaac aacgtttgca gatgggcaaa ctctttctca cttaaacggt
     7021 gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc cgcgctccag tgattctcac
     7081 ccacaccatt aagctgcgcc accagcattt taacatcata aatttgcagc agtttctcca
     7141 gcattgcctg tgcggcattc ccggtgctgt atgaatcggt tactgatata ttttcctgca
     7201 tagatttaac cggccatctg agatggccga caggttaaca taattactgc cgtttagggc
     7261 cacgcgctca gccagcgggt gaaaacgcat ctatcacctt gtgatcgata ccttcattct
     7321 cccccgccag ttcggcaccg agcttcgcca tcaaacagcg ccaagctcaa tgcccctgaa
     7381 acggcaaata cgcgcgccag gccaatcgcc cccagggctt ccagccgatc cgcatcctgg
     7441 acgattttag cttccgttgt taagggggcg atttgcgcgc tgaaactgtg agcggcaatg
     7501 gcatgacaaa cggcctcaat tttctccgcc ggaaattgca cgaactcttc acggagcagg
     7561 cgacgcgtct cttctgccgc caggattgaa gaacgctgcc gttgcggatg atttttcgcc
     7621 aggctcacga tatcgtgaaa ataacaggcg gttaaaatca ccagcatatc gacatcgtca
     7681 tctgccgcca gtttctgtgc cgtagcccag acacggcgaa aatgacacag gtcatgcgcc
     7741 gcgtcctgat gttgatgatg gtttttcaac cagttctcga actatgcctg ccagtgttgt
     7801 aactccatac gcgctccgtt gccaaagagt ctcaacctta gcagttttta ttcatagcgt
     7861 ctgcaacgtg cggaacgagg tcgggtgtac catgcaatgc cgcctaacaa ggcaccaacc
     7921 agggccataa acagaaatcc gaccaatgct cctaaccact tccccgcggt agatcctaaa
     7981 tcactttcaa atcccgacac gggggcatcg gggatccggc tcatcaccca gatgtatcgc
     8041 gccattgccc agacgaaata gccacaccat gtatagaaag ccc
//
LOCUS       DAZOYF010000173         8050 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZOYF010000173 DAZOYF010000000
VERSION     DAZOYF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.174"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..105
                     /gene="yjbL"
                     /locus_tag="ACHUOF_003565"
     CDS             <1..105
                     /gene="yjbL"
                     /locus_tag="ACHUOF_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR0612589.1"
                     /translation="YAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            129..836
                     /locus_tag="ACHUOF_003566"
     CDS             129..836
                     /locus_tag="ACHUOF_003566"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR0612590.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            1199..2236
                     /gene="dusA"
                     /locus_tag="ACHUOF_003567"
     CDS             1199..2236
                     /gene="dusA"
                     /locus_tag="ACHUOF_003567"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR0612591.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2370..2612
                     /gene="pspG"
                     /locus_tag="ACHUOF_003568"
     CDS             2370..2612
                     /gene="pspG"
                     /locus_tag="ACHUOF_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR0612592.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(2778..3761)
                     /locus_tag="ACHUOF_003569"
     CDS             complement(2778..3761)
                     /locus_tag="ACHUOF_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR0612593.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3844..5259
                     /gene="dnaB"
                     /locus_tag="ACHUOF_003570"
     CDS             3844..5259
                     /gene="dnaB"
                     /locus_tag="ACHUOF_003570"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0612594.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5312..6391
                     /gene="alr"
                     /locus_tag="ACHUOF_003571"
     CDS             5312..6391
                     /gene="alr"
                     /locus_tag="ACHUOF_003571"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR0612595.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6644..7837
                     /gene="tyrB"
                     /locus_tag="ACHUOF_003572"
     CDS             6644..7837
                     /gene="tyrB"
                     /locus_tag="ACHUOF_003572"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR0612596.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1978 a         1889 c         2081 g         2102 t
ORIGIN      
        1 tatgcagcgc tgcgtcatcg ggatataatt attgcaaaaa tagatttgtt tattcaacga
       61 atcacgcata atttatggca tgcacgtaaa aaaatgtgtt tttaattgag cacataaatg
      121 atctcaaaat gtgggtcaat aagtatactg acgattgcac tgatgaggat ttaaacgatc
      181 gtgactttat tgcatcagtt gttgaccggg ctatttttca tttcgcgatt aatagtatat
      241 gtaatcctgg ggataataaa gatgcaatgc ccattgaaca atgtactttt gatgtagaaa
      301 ctaagaatgg ccttccctcc acggttcagc tattttatga ggaatctaag gataatgaac
      361 ctttagcgaa tatacatttt caagcaatag gttctggttt tttaacgttt gttaatgcct
      421 gccaggaaca tgatgacaac agcttaaaat tatttgcttc gctgttaatt tcactttcat
      481 attctagtgc ctacgcagat ttatcagaaa cagtgtatat taatgaaaat aatgagagct
      541 acctgaaagc tcagtttgaa aaattatatc aacgtgatat gaagaagtac ctgggagaga
      601 tgaagcgtct ggcagatggg ggagaaatga attttgatgg ctatctggat aagatgtcac
      661 atctggtgaa tgaaggaacg ctcgatcctg atattttaag caaaatgcga gatgctgcac
      721 cacaattaat tagcttcgcg aagtcgtttg acccaacctc aaaggaagag attaaaatac
      781 ttacagacac ttctaaatta atttatgact tgttcggggt taaatcggag aaataatatg
      841 tgaagttctt cgatagtatg gaaggcatta tataaaagga cccaatattt attgggttct
      901 tttttctcta tcaatgctat tagcagggag atatatcacc agagtttaat gtgtgatttt
      961 ttatttatcg tcgaacctgg attgtttatc attggcctta acaaagttaa cggctaataa
     1021 gattatttcc atcacttcgt gagagcttca tgccttgaga ggatctcaat tttcttttgc
     1081 aatgagacag gcgcttcctg ttgttatggt atagtacccc gctattgagc ctcctgaaca
     1141 gtgaagctga ataacataac cccatgatat atcgataaaa taatctctac atttgaaaat
     1201 gcacggtaat tctgaaatgc aaaaaatcaa ccaaaccagc gcaatgcctg aaaaaactga
     1261 cgttcactgg agtggtcggt ttagtgttgc accaatgctc gactggacgg acagacattg
     1321 ccgctatttc ttgcgtctgc tttcccgcaa tacgttgctt tataccgaaa tggtgaccac
     1381 aggggcgata attcacggta aaggtgatta tctggcgtac agtgaagaag aacatccggt
     1441 agcgttgcaa ctgggcggta gcgatccggc ggcgctggcg cagtgtgcaa agctggcaga
     1501 agcgcgcgga tatgatgaga tcaacctgaa tgtcggctgc ccgtctgacc gggtgcagaa
     1561 cggcatgttt ggtgcgtgtc tgatgggtaa tgcgcagctg gttgccgact gcgtgaaagc
     1621 gatgcgcgat gtggtgtcga ttccggtgac ggtgaaaacg cgtattggca tcgacgacca
     1681 ggacagctat gaatttctct gcgatttcat caataccgtt tccggcaaag gcgagtgtga
     1741 gatgttcatc atccacgcac gtaaagcctg gctttcgggg ttaagcccga aagaaaaccg
     1801 tgaaatcccg ccgctcgatt atccgcgtgt gtatcaactg aagcgtgact tcccgcatct
     1861 gacaatgtcg attaacggtg gtatcaagtc gctggaagaa gctaaagcgc atttgcaaca
     1921 tatggatggc gtgatggtcg ggcgcgaggc gtatcagaat ccgggtattc tggcggcggt
     1981 agaccgggag atctttggtt cctcggatac cgatgccgat ccggtggcag tagtgcgtgc
     2041 catgtatccg tacattgagc atgaactcag ccaggggacg tatctcggcc atattatccg
     2101 gcatatgctg ggtttgttcc agggtattcc tggcgcgcgg cagtggcggc gttatttaag
     2161 tgaaaatgcc cataaagcgg gtgctgacat taacgtgctg gaacacgcgc tcaaactggt
     2221 ggcggataag cgttaacttt tcaccaaaaa gtagtcaaat tcaccacgcc ctgcgcaccg
     2281 tcgcggggcg ttttgctgtt aaatcaatag attatttttg gcatgattct tgtaatgcca
     2341 gcaagagatt tcatatttgg gagagcatca tgctggaact actttttgtg attggctttt
     2401 ttgtcatgct gatggtcacc ggcgtttcgt tgctgggcat tatcgccgcg ctggttgtgg
     2461 cgacggccat tatgttcctc ggcggtatgc tggcattgat gattaagttg ctgccgtggt
     2521 tactactggc gattgcggtg gtgtgggtta ttaaggcgat taaagcacca aaaatgccga
     2581 aatatcagcg ttatgaccgc tggcgttact aagggattgt gcggatgatc acaacctaag
     2641 gttttatcct tagaacaaaa taggaattga taatcaaatc tgtcactatt gcgcctctaa
     2701 cagattcatc gtgctgtacc ctacatacag ccgaactata aaaagaaagg gcttcccagg
     2761 tggaagccct atttctttta tggaatcagc aggctggaac cttgcgtcgc ccggctttcc
     2821 agaatctcat gcgcacgctg cgcatccttc agcggatatt tctgctgctc ggcgacatcg
     2881 accttaatca caccgctggc aatcaaagag aacagttcat tactggcctc ggttaattcc
     2941 tcccgcgtgg tgatatagcc ttgcagggaa gggcgtgtca catacaacga gcctttttga
     3001 ttgagaatgc ctaagttcac accggtaacc gcacctgatg agttgccaaa actgaccatt
     3061 aagccgcggc gttgcaggca atccagcgac cgttcccagg tgtctctgcc cacggaatcg
     3121 tacactacgc gcactttctt accgccggtg atctctttca atcgctcgac cagattctct
     3181 tcacgatagt taataacctg ccacgcgccg gcttttagcg cgctctgcgc tttttgcgcg
     3241 gttcctacgg tgccgatgag tttcgcgccc agggcttttg cccactggca ggcaattaag
     3301 ccaatgccgc cagccgctgc gtggaacagg aactgttcat cgggtttaat ttcataggtt
     3361 ttgcgcagca gataataaac cgttaagcct ttcaggaagg atgccgcagc ttgctcaaaa
     3421 gaaattgccg caggcagaat cgccgcttta tccgcattaa tgttatgcac agagctgtaa
     3481 gcgcctaacg ccgactgcgc atagactaca tgatcgccag ccttaatatg ctttacacca
     3541 ctgccgactt tactcacgat gcctgccgct tcggtaccta atccgctggg tagcgatggc
     3601 ggcgggtaaa ggccgctgcg gatatacgtg tcgataaaat tgatgccgat ggctttattt
     3661 tcgacctgga tttcattctc cgccggatcg gcaggagtga actctacggc ttgaagtact
     3721 tccgggccac cgtgcttgtg aaattcaatt cgtgttgcca tgtgtcctcc agaacgtatc
     3781 gtcagggtct gcttcatatg gtaaagtttc gacccattct ttatttcggt aactccattc
     3841 actatggcag gaaataaacc cttcaacaaa cagcaggctg aaccccgcga acgcgatcca
     3901 caagttgccg ggctgaaagt gcctccgcac tcgatcgaag cggagcagtc ggtgttgggc
     3961 ggtttaatgc tggataacga acgctgggat gatgtagccg agcgtgtggt agcagacgat
     4021 ttttacaccc gcccacaccg tcatatcttt actgaaatgg cgcgtttgca ggaaagcggt
     4081 agtcctatcg atctgattac ccttgcggaa tcgttggaac gccaggggca actcgatagc
     4141 gtcggtggtt ttgcttatct ggcagagctg tcaaaaaata cgccaagtgc ggcgaacatc
     4201 agtgcttatg cggacatcgt gcgtgaacgt gccgttgtcc gtgagatgat ctcggttgcg
     4261 aatgagattg ctgaagccgg ttttgatccg caggggcgta ccagcgaaga tctgctggac
     4321 cttgctgaat cccgcgtctt taaaattgcc gaaagtcgtg caaacaaaga cgaagggccg
     4381 aagaacatcg ccgatgtgct cgacgcaacc gtggcgcgta ttgagcagtt gtttcagcag
     4441 ccacacgatg gcgttaccgg ggtaaacacc ggttatgacg atctcaacaa aaaaaccgct
     4501 ggcttgcagc cgtcggattt gatcatcgtc gccgcgcgtc cgtcgatggg taaaacaaca
     4561 tttgcgatga acctcgtcga aaacgcggca atgttgcagg ataaaccagt acttatcttc
     4621 tcgctggaga tgccatcaga acagatcatg atgcgttctc tggcgtcgct gtcgcgcgtt
     4681 gaccagacta aaatccgtac cgggcagctc gatgatgaag actgggcgcg catttccggc
     4741 accatgggta ttttgctcga aaaacgcaat atctatatcg atgactcttc cggcctgacg
     4801 ccaacggaag tgcgttcccg cgcacgccgt attgcccgtg aacacggcgg catcgggctt
     4861 atcatgatcg actacctgca actgatgcgc gtaccggcgc tttccgataa ccgtacgctg
     4921 gaaattgcag aaatctcccg ctcgctgaaa gcactggcga aagaactgaa cgtgccggtg
     4981 gtggcgctgt cccagttgaa ccgttctctg gaacaacgtg ccgacaaacg tccggtcaac
     5041 tccgacctgc gtgaatctgg ctctatcgag caggatgcgg acttgatcat gtttatctat
     5101 cgtgatgagg tgtatcacga aaacagtgat ttaaaaggca tcgcggaaat tattatcggt
     5161 aaacaacgta acggcccaat cgggacggta cgcctgacct ttaacggtca atggtcgcgc
     5221 ttcgacaact atgcggggcc gcagtacgac gacgaataat aattatttta tgaattaggt
     5281 aattaaagca aacaattatc aaggaacaca aatgcaagcg gcaactgttg tgattaaccg
     5341 ccgcgctctg cgacacaacc tgcaacgtct tcgtgaactg gcacctgcca gtaaaatggt
     5401 tgcggtggtg aaagcgaacg cttatggtca cggtcttctt gagaccgcgc gaacgctccc
     5461 cgatgctgac gcctttggcg tagcccgtct cgaagaagct ctgcgactgc gtgcgggggg
     5521 aatcaccaaa cctgtactgt tactcgaagg cttttttgat gccagagatc tgccgacgat
     5581 ttctgcgcaa cattttcata ccgccgtgca taacgaagaa cagctggctg cgctggaaga
     5641 ggctagcttg gacgagccgg ttaccgtctg gatgaaactc gataccggta tgcaccgtct
     5701 gggcgtaagg ccggaacagg ctgaggcgtt ttatcatcgc ctgacccagt gcaaaaacgt
     5761 tcgtcagccg gtgaatatcg tcagccattt tgcgcgcgcg gatgaaccaa aatgtggcgc
     5821 aaccgagaaa caactcgcta tctttaatac cttttgcgaa ggcaaacctg gtcaacgttc
     5881 cattgccgcg tcgggtggca ttctgctgtg gccacagtcg cattttgact gggtgcgccc
     5941 gggcatcatt ctttatggcg tctcgccgct ggaagatcgc tccaccggtg ccgattttgg
     6001 ctgtcagcca gtgatgtcac taacctccag cctgattgcc gtgcgtgagc ataaagccgg
     6061 agagcctgtt ggttatggtg gaacctgggt aagcgaacgt gatacccgtc ttggcgtagt
     6121 cgcgatgggc tatggcgatg gttatccgcg cgccgcgccg tccggtacgc cagtgctggt
     6181 gaacggtcgc gaagtaccga ttgtcgggcg cgtggcgatg gatatgatct gcgtagactt
     6241 aggtccacag gcgcaggaca aagccgggga tccggtcatt ttatggggcg aaggtttgcc
     6301 cgtagaacgt atcgctgaaa tgacgaaagt aagcgcttac gaacttatta cgcgcctgac
     6361 ttcaagggtc gcgatgaaat acgtggatta atcgttctgt aatatttgat tgtctgtgcc
     6421 ggatgcggcg tgaatgcctt atccggccaa taaaatccta aaaattcaat aagttgatgt
     6481 tctttcatgc tcttataaag gtcgtgcctc tggcggatgt acgtttatca ttagtctcac
     6541 tctgttgcta attgccgttc gctcctgaac atccactcga tcttcgcctt cttccggttt
     6601 attgtgtttt aaccacctgc ccgtaaacct ggagaaccat cgcgtgtttc aaaaagttga
     6661 cgcctacgct ggcgacccga ttcttacgct tatggagcgt tttaaagaag accctcgcag
     6721 cgacaaagtg aatttaagta tcggtctgta ctacaacgaa gacggaatta ttccacaatt
     6781 gaaagccgtg gcggaggcgg aagcgcgcct gaatgcgcag ccccatggcg cttcgcttta
     6841 tttaccgatg gaagggctta acagctatcg ccatgccatt gcgccgctgc tgtttggtgc
     6901 ggaccatccg gtactgcaac aacagcgcgt agcaaccatt caaacccttg gcggctcagg
     6961 ggcattgaaa gtgggcgcgg atttcctgaa acgctacttc ccggaatcag gcgtctgggt
     7021 cagcgatcct acttgggaaa accacgtagc aatattcgcc ggggctggat tcgaagtgag
     7081 tacttacccc tggtatgacg aagtgactaa cagcgtgcgc tttaatgacc tgttggcgac
     7141 gctgaaaaca ttacctgccc gcagtattgt gttgctgcat ccatgttgcc acaacccaac
     7201 gggtgccgat ctcactaatg atcagtggga tgcggtgatt gaaattctca aagcccgcga
     7261 gcttatccca ttcctcgata ttgcctatca aggatttagt gccggtatgg aagaggatgc
     7321 ctacgctatt cgcgccattg ccagtgctgg attacccgct ctggtgagca attcgttctc
     7381 gaaaattttc tccctttacg gcgagcgcgt cggcggactt tctgttctgt gtgaagatgc
     7441 cgaagccgct ggccgcgtac tggggcaatt gaaagcaaca gttcgccgca actactccag
     7501 cccgccgaat tttggtgcgc aggtggtggc tgcagtgctg aatgacgagg cattgaaagc
     7561 cagctggctg gcggaagtag aagagatgcg tactcgcatt ctggcaatgc gtcaggaatt
     7621 ggtgaaggta ttaagcacag agatgccaga acgcaatttc gattatctgc ttaatcagcg
     7681 cggcatgttc agttataccg gtttaagtgc cgctcaggtt gaccgactac gtgaagaatt
     7741 tggtgtctat ctcatcgcca gcggtcgcat gtgtgtcgcc gggttaaata cggcaaatgt
     7801 acaacgtgtg gcaaaggcgt ttgctgcggt gatgtaatgc aggaaagcag gctggagtta
     7861 cccagcctgc agtgaaatta aactgtcgtc gctttcacta tttctttgta gatgattttt
     7921 ttgatgccgt tgttctacgt gagagataat aaaacgttgt cagttctttt attgttaagt
     7981 ttatcccaat tatctggaat tcctttccct attttttcgt gtggagtaat aatttctgca
     8041 catctaacca
//
LOCUS       DAZOYF010000174         8037 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZOYF010000174 DAZOYF010000000
VERSION     DAZOYF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.175"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(437..922)
                     /gene="sixA"
                     /locus_tag="ACHUOF_003573"
     CDS             complement(437..922)
                     /gene="sixA"
                     /locus_tag="ACHUOF_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR0612597.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1125..3269)
                     /gene="fadJ"
                     /locus_tag="ACHUOF_003574"
     CDS             complement(1125..3269)
                     /gene="fadJ"
                     /locus_tag="ACHUOF_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR0612598.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3269..4579)
                     /gene="fadI"
                     /locus_tag="ACHUOF_003575"
     CDS             complement(3269..4579)
                     /gene="fadI"
                     /locus_tag="ACHUOF_003575"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR0612599.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4759..5043)
                     /locus_tag="ACHUOF_003576"
     CDS             complement(4759..5043)
                     /locus_tag="ACHUOF_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR0612600.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5415..6755
                     /gene="fadL"
                     /locus_tag="ACHUOF_003577"
     CDS             5415..6755
                     /gene="fadL"
                     /locus_tag="ACHUOF_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR0612601.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            7121..>8037
                     /gene="yfdF"
                     /locus_tag="ACHUOF_003578"
     CDS             7121..>8037
                     /gene="yfdF"
                     /locus_tag="ACHUOF_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFR0612602.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKKGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKV"
BASE COUNT         2176 a         2026 c         1814 g         2021 t
ORIGIN      
        1 acatccttaa aaaatacgga ataagtattt agaaaaaata gaaagtatat caataatgaa
       61 tatcaacagc ttaaaaaaca aacaatgaac atataatgcg attggcattt atccactttt
      121 gacaatataa tatataaaca ctatagccag aatttaatca agtttcaatt acatcctgcc
      181 tgattattca actctatttt ctctattatc tgtattaata aatgacaatg agtgtcaaac
      241 agatattatt catttcaaca tgtaactttg ggcaaaatca agatacgcgt agaagaataa
      301 tttccgaatg aatgtagaaa gtgttttatt tatagataag gagagtcttc gatagtagtg
      361 aggttgacag atatccgctg cctaatgagc aattatgccc aatgaatcta cctcattggg
      421 cataatttgg aacacatcag atagcttttg ccatcttcag attacacggg ctcatctgcc
      481 agttgaatgt accgtttccg ctttcatcaa gcgttacgct ggcaatggcc gaggtggtaa
      541 acataggcgg cgtttcgccc ggacataact cggcaaccag atacccgact aacggtaagt
      601 gagagatcac cagtaccgaa gcaacacctt cattggtcaa cgcctgcaaa taggcactga
      661 ccagaccaac atcgccgcag ggcgttaact ccggcagaac ttctgcactg gaaggcaggt
      721 tcagacaatc ccctacttct tccagtgttt gctcggctcg caggaacggg ctcaccagaa
      781 cgcgttcgat ttccactttt tgacctttca gccagttcgc catcaggcga gattcgtcac
      841 aaccattagt ggtcagagga cgaaccgaat cactggcgga atcgagggct gcgtcgccgt
      901 gacgcatgat aaaaacttgc atattgcacc gcttttgtta accagattcg cccgttttct
      961 ttcatctgaa accagagaaa gaaaacggtg gccggcattg tgccttatcc attcaccgaa
     1021 tgaaacgctg ttttttacct caatggcgta agtatagtca atatgcgttt acattttgac
     1081 caacactccg ccattcagcg cggattcata tagctttgac cttcttattg caggtcagtt
     1141 gcagttgttt tccaaaaact ttccccacgc gcgcccatct cgaccaaacg ctcgcaaggg
     1201 gtaaaacggg aaccatactg cgtggccagt cgttgcatta ttgcaaccac ttcgcccgcg
     1261 ccgagagaat cgatatagcg gaacggtcca ccgagaaatg gcggaaaacc aatgccaaat
     1321 accgcaccaa tatccccgtc acgcacgcta cggataacct gctcatcaac acaacgtact
     1381 gcttcattca gcatcaacat cacacaccgt tcagcaacct gcggtgcgga gagtcgcccc
     1441 tgcccttgtg cgccaatgag cgggtaaatg gcaggatcga cctgtttttt gcttttacgc
     1501 cctttctgac cataaagata gaaaccccga ccattttttc tgcctttgcg atcgtcgttc
     1561 aaaattgaag aaacaacatt tgcaggcgcg ctaaaacgtt ctccataagc ggcttccagt
     1621 acaggaataa ttttagtccc ggtgtcgatt cctacctcat ccaaaagttg gattgggcct
     1681 accggaaaac caaatttcac tagcgcggca tcaatgtgct ctacccgttc accttcggtc
     1741 agcatgcgga tagcttcatt aatgtaaggc gctaagatgc gattgacgta aaacccggct
     1801 ttgtcacgca cgacaatggg cgttttgccc tgttttttcg ccagttttac cgtagtggcg
     1861 atggtttgcg ccgatgtacc cgcatgagga ataatctcta ccagcggcat tttttccacc
     1921 ggactgaaga aatgcaggcc aataacttgc tcaggtcgcg cggcgtgagc ggcgatatca
     1981 ccaatcggta aagatgacgt gttcgaagca aaaatggtat gagcggcgca attttgctcc
     2041 acttccgcca ccatctgttg ttttaattcg agattttcaa acaccgcttc aataatcaga
     2101 tcgcgatggg caaagccgcg atagtccgtc gttccggaga ttaatgccag ctgtttgtca
     2161 cgttcgctgg ctttgagatg acgacggcga actttgccct ccagctgatc ccaactgtac
     2221 ttcagcgcat gatttatgcc ctgcgggttg atatctttaa ttctgaccgg aagccccgct
     2281 ttacaagcag tgacataagc aataccgccg cccatcaagc caccacctaa aattcccacg
     2341 ctgtttaatg gcgcaggcgg tgcatcacta ccgggatctt tcttcacgtc cgtactggca
     2401 aaaaagatac tacgcaaagc ttgcgattgc ggcgtcatcg ccagttcgcc aaaggcgcga
     2461 gcttcagcgt catagccgct gctggtgccc tgcgctaatc ccgtttcaac aacctccagg
     2521 atgcgttctg tcgccggata attgccttga gttttgtgtt ctgttttctt gccgaccatt
     2581 ttgaacagca gcgcacgacc taacggcccc gccagaatac gctcgcgtac aggtagaggg
     2641 cgggaagatg ggcgatcctt cttcgccagc tcaacagcgg cttccagcaa aatggagtgc
     2701 ggaacaacgt catccaccag ccccagcttt aatgcctgtt tcgcccgaag ttgttttccg
     2761 gtgagaatca tctctaatgc tgtactgacg cctatcagac gcggtaaacg ctgggtgccg
     2821 cctgaaccgg gtaacaatcc aagttgtact tcaggcaaac cgagcaccgt tttaggatcg
     2881 tcagtacaaa cgcgaccgtg gcacgccagc gccagctcca gcccaccgcc caggcaagcg
     2941 ccatgaatag ccgcgataac cggaatgggc aaagcatgaa tctccgccat caactgttgg
     3001 ccctgccgcg ccagtgcttc cgcttcttgc gccgttttgc agttgccaat catgttgatg
     3061 tctgctccgg cgataaaatt gtccggtttc gcggagataa acaccacgcc gcgcaactct
     3121 ttgttttcac ggagttgctt aataatggcg cgcacctgcg aggcaaattc cgccttcagg
     3181 gtattcattt tctcatccgg tacgtcgatg gtgatgatag caatgttgtc cagacgaaca
     3241 ttaagagtaa acgctgatgc catttccatt attccgcctc cagaaccatt gccgcgccaa
     3301 gcccaccggc agcacaggcg gtaactaaac caaatccacc gccgcgacgg cgaagttcat
     3361 gcaatgtctg ggtaatcatc cgcgcgccgg tcgccgcgaa gggatgtccg taagcaatcg
     3421 aaccgccaag cacgttaaat ttgctatcgt ccacttcgcc agtggcatgt gcacgcccca
     3481 gtacttcacg agcaaaacgt tcactaccca gcaactgaat attcgccagt gtctgagctg
     3541 caaaggcttc gtgcatatcg atcaatgtca gttcgctcat cgtcaaaccg gcacgttcca
     3601 gcgccagcgg tgttgaccag gctggaccga gcaacatgtc ctgccagaca tcaatcgcag
     3661 taaatgcgta gctgcgcaga taccccagcg gcaccagccc taattctttc gcccgggatt
     3721 cagtcatcag gatcaccgct gccgcgccat cggtcagcgg cgtactgttt gccgccgtta
     3781 ccgttccgtg tttgcgatca aacgccgggc gcagctttgc gtaatcggca agcgaggaat
     3841 taccgcgaat attgttgtct tcgacaagcg gttgtttata aggagggata aaggcagtca
     3901 tcacctcttc tttgagtttt ccgtctgacc atgcctgagc ggcacgctga tgcgaacggt
     3961 gcgctaatgc atcttgctgt tctcgggtga tgccgtaggt tttcgccatt tgttcggcgg
     4021 tgtcgcccat ccgcaagcca gtagaatatt ctgctaccgc aggcggtacg ggcattaagt
     4081 cgcgcaaacg caggcgagag aagagtttca ggcgctggct catggttcga gctttgttga
     4141 catcaaccag cacgtacgcc agttttttac tgacgccaat tggcaatacg gaagaggaat
     4201 ctgccccacc ggcaatcccc gctcggatag ttcccgccat caggctttct gcgacgtttg
     4261 caaccgcttg gaaactggta gcgcaagcgc ggctgacgct gtaagcatcg gtatgcacat
     4321 tcattccggt accgagaaca atttcacgcg caatgttggg ggcttcaggc atttgtacga
     4381 cctgaccaaa gaccagctgt tcaatcactt cagcggggat ctcgctgcgt gccagcagtt
     4441 cgcctaccac catcttccct aaatcaaccg tgggaatgcc atgaaaagcc gtcgcctggc
     4501 gggcaaaagg cgtacgtaaa ccgctaacaa tggcgatacg atcgccctgg cgggtaacca
     4561 gtggtaaaac ctgacccata acactcccct gtaaaaaaat aaataaagtg gtctgacctg
     4621 atcatagtct taaccatttt tttacattta gccaagcgga gaaaaaggaa agtgggagct
     4681 atgacacagt gaagacagaa gaaagaaaag aaaacgcccc tgccagcaac tgacaggggc
     4741 gtacgtaccc gagaggaatt aacgcagacc cagctggaag atcagcattt cagcttcgca
     4801 ggcaaaagtg aaatcgatat ccaggcgcac accgtcagac tcttcagtga aagtcgggga
     4861 gattttgcac ggttcggatt tcacgctacg ggatttttca gtcagcgccg ccagcgtttg
     4921 ttctgcttct gcgcggtttg caaacacgcg gctgtaagac gcggtgcagt cggagttgtc
     4981 cataatggtg ccaacatcca tacagcagca aaccggggtt tcatcagcac tacatttact
     5041 catcgttgat ttcctctgta tgtgcaccca aggtgccaga taaacgttgt ggatatttta
     5101 cgcttccgga aagtgctgct ccagttgtta attctgcaaa atcggataag tgaccgaaat
     5161 cacacttaaa aatgatctaa aacaaaattc acccgaatcc gcgagtgcgc aacctccaaa
     5221 ttttgccagc tggatcgcgt ttcttagatc atatttgaaa aaagatggaa acatacttgc
     5281 aacattctag ctggtccgac ctatactctc gccactggtc tgatttctaa gatgtacctc
     5341 agaccctaca cttcgcgctc ctgttacagc acgtaacata gtttgtataa aaataaatca
     5401 ttgaggttat ggtcatgagc cagaaaaccc tgtttacaaa gtctgctctc gcagtcgcag
     5461 tggcacttat ctccacccag gcctggtcgg caggctttca gttaaacgaa ttttcttcct
     5521 ctggcctggg ccgggcttat tcaggggaag gcgcaattgc cgatgatgca ggtaacgtta
     5581 gccgtaaccc cgcattgatt accatgtttg accgcccgac attttctgcg ggtgcggttt
     5641 atattgaccc ggatgtgaat atcagcggaa cgtctccgtc tggtcgtagc ctgaaagccg
     5701 ataacatcgc gcctacggca tgggttccga acatgcactt tgttgcaccg attaacgacc
     5761 aatttggttg gggcgcttct attacctcta actatggtct ggcaacagag tttaatgata
     5821 cttatgcagg cggctctgtc gggggtacaa ctgaccttga aaccatgaac ctgaacttaa
     5881 gcggtgcgta tcgcttaaat aatgcatgga gctttggtct tggtttcaac gccatctacg
     5941 ctcgcgcgaa aattgaacgt ttcgcaggcg atctggggca gttggttgct ggtcagatta
     6001 tgcaatctcc tgccgggcaa actcctcaag ggcaagcatt ggcagctacc gccaacggta
     6061 tcgacagtaa taccaaaatc gctcatctga acggtaacca gtggggcttt ggctggaacg
     6121 ccggaatcct gtatgaactg gataaaaata accgctatgc actgacctac cgttctgaag
     6181 tgaaaattga cttcaaaggt aactacagca gcgatcttaa tcgtgcgttt aataactacg
     6241 gtttgccaat tcctaccgcg acaggtggcg caacgcaatc gggttatctg acgctgaacc
     6301 tgcctgaaat gtgggaagtg tcaggttata accgtgttga tccacagtgg gcgattcact
     6361 atagcctggc ttacaccagc tggagtcagt tccagcagct gaaagcgacc tcaaccagtg
     6421 gcgacacgct gttccagaaa catgaaggct ttaaagatgc ttaccgcatc gcgttgggta
     6481 ccacttatta ctacgatgat aactggacct tccgtaccgg tatcgccttt gatgacagcc
     6541 cagttccggc tcagaatcgt tctatctcca ttccggacca ggaccgtttc tggctgagtg
     6601 caggtacgac ttacgcattt aataaagatg cttcagtcga cgttggtgtt tcttatatgc
     6661 acggtcagag cgtgaaaatt aacgaaggcc cataccagtt cgagtctgaa ggtaaagcct
     6721 ggctgttcgg tactaacttt aactacgcgt tctgataacg cgttcgcctg gataaagtca
     6781 cctgctatgc aggtgacttt aactccccca cttcaccgaa agtagtttcc tcattgcttg
     6841 cctcctgagt tttgcaaaca gcctgatggc agcttgccct ttcaaatcag taagtggtat
     6901 ccacagacaa acctgaaggg aaatgcatat tttcaagcgt tctgctcgtc cttctcaaag
     6961 agtttacttt tctgcatttc caggatactc aacccccctg gctattgtgc gcttatacac
     7021 tcaaattaaa gataggttct aaataaatga gcgttttttg atagtctatt tcattaggta
     7081 atatatattt gtaacaaatc aatcaacatg gaataaaatc atgctaccat ctatttcaat
     7141 caacaatacc agcgcagctt acccagaatc catcaatgaa aataacaatg atgaaattaa
     7201 tggattagta caagagttca aaaacctttt taatggtaag aaaggaataa gcacctgtat
     7261 taaacatcta cttgagctta taaaaaacgc catacgagta aacgacgatc cttatagatc
     7321 taatattaat aattcctcag ttacttatat tgatattggc tccaatgata cagaccatat
     7381 tactattggt atcgacaacc aagaaccaat agaattacct gcgaactata aagacaaaga
     7441 actcgtccgt actatcatta atgacaacat agttgagaag actcatgata tcaataacaa
     7501 ggaaatgatc ttcagcgcat taaaagaaat atatgatgga gatcctggtt ttatcttcga
     7561 taagatatca cacaaactca gacatacggt aacggaattt gatgagagcg ggaaaagcga
     7621 accaactgac ttatttacct ggtacggtaa agataaaaaa ggcgactctc tcgctattgt
     7681 aattaaaaat aaaaacggaa atgattactt atctctcggt tactacgatc aggacgacta
     7741 ccacattcaa agaggaattc gtattaatgg tgatagtctc acccaatatt gtagtgaaaa
     7801 cgccaggagt gcttcagcgt ggtttgaaag cagtaaagct atcatggcag aatcatttgc
     7861 aactggttcc gatcatcagg ttgtaaacga gctcaacggg gaaagactga gagaacctaa
     7921 cgaagttttt aaacgtttag gtcgagcaat aagatataat tttcaagtgg acgatgcaaa
     7981 atttagacgc gataatgtaa aagaaataat ttctacttta ttcgctaaca aagttga
//
LOCUS       DAZOYF010000175         7986 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZOYF010000175 DAZOYF010000000
VERSION     DAZOYF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.176"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHUOF_003579"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHUOF_003579"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR0612603.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHUOF_003580"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHUOF_003580"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR0612604.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHUOF_003581"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHUOF_003581"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR0612605.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="ACHUOF_003582"
     CDS             6038..6658
                     /locus_tag="ACHUOF_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR0612606.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="ACHUOF_003583"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="ACHUOF_003583"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR0612607.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
BASE COUNT         1812 a         2324 c         2031 g         1819 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaac
//
LOCUS       DAZOYF010000176         7745 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZOYF010000176 DAZOYF010000000
VERSION     DAZOYF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.177"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..1014
                     /gene="ydfJ"
                     /locus_tag="ACHUOF_003584"
     CDS             <1..1014
                     /gene="ydfJ"
                     /locus_tag="ACHUOF_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFR0612608.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1103..2563
                     /locus_tag="ACHUOF_003585"
     CDS             1103..2563
                     /locus_tag="ACHUOF_003585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0612609.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2599..2802)
                     /gene="ydfZ"
                     /locus_tag="ACHUOF_003586"
     CDS             complement(2599..2802)
                     /gene="ydfZ"
                     /locus_tag="ACHUOF_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR0612610.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2980..3666)
                     /gene="rspR"
                     /locus_tag="ACHUOF_003587"
                     /gene_synonym="ydfH"
     CDS             complement(2980..3666)
                     /gene="rspR"
                     /locus_tag="ACHUOF_003587"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR0612611.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3755..4501)
                     /gene="ydfG"
                     /locus_tag="ACHUOF_003588"
     CDS             complement(3755..4501)
                     /gene="ydfG"
                     /locus_tag="ACHUOF_003588"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR0612612.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4638..6683
                     /gene="dcp"
                     /locus_tag="ACHUOF_003589"
     CDS             4638..6683
                     /gene="dcp"
                     /locus_tag="ACHUOF_003589"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR0612613.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6728..7246)
                     /locus_tag="ACHUOF_003590"
     CDS             complement(6728..7246)
                     /locus_tag="ACHUOF_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR0612614.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7522..>7745
                     /gene="ydeI"
                     /locus_tag="ACHUOF_003591"
     CDS             7522..>7745
                     /gene="ydeI"
                     /locus_tag="ACHUOF_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR0612615.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT         1941 a         1812 c         1957 g         2035 t
ORIGIN      
        1 atcttgctgg tgacgctgcg tattattcag ggattgggtg caggtgcgga aatttccggt
       61 gccggtacga tgctggcgga atatgcgcca aaaggtaagc gcggaattat ctcctcattt
      121 gtggctatgg gaactaactg cggcacctta agcgcaacgg caatctgggc ctttatgttc
      181 ttcattctca gtaaagagga actgctggcg tggggatggc gtataccgtt cctggcgagc
      241 gttgtcgtga tggtctttgc tatctggttg cgtatgaatc tgaaagaaag cccggtcttt
      301 gagaaggtta acgacagcaa ccaaccgaca gcaaaacctg cacctgctgg tagcatgttc
      361 cagagcaaat ccttctggct ggcaacaggg ctgcgttttg gtcaggcggg taactccggt
      421 ttaattcaga ctttccttgc aggctattta gtacagacgt tattgtttaa caaagcaatt
      481 ccaacagatg cattgatgat cagttcgatt ctcggcttta tgaccattcc gttccttggt
      541 tggttatccg ataaaattgg tcgccggatc ccgtatatta ttatgaatac ctctgcgatt
      601 gtgctggcgt ggccaatgct ttctatcatc gtagataaaa gctatgcccc gagcaccatt
      661 atggttgcac tgattgtgat tcataactgt gcggtgctgg gattatttgc tctggaaaac
      721 attaccatgg cagaaatgtt cggctgtaaa aaccgcttca cccggatggc tatttcaaaa
      781 gaaattggtg gtcttatcgc ttccggtttt ggtcctatcc tggcgggtat tttctgcacc
      841 atgacggaat cctggtatcc gatcgccatt atgatcatgg catattcagt gattggttta
      901 atctctgcgc tgaaaatgcc agaggtgaaa gaccgtgatt taagtgcgct ggaagacgct
      961 gcggaagatc aaccgcgtgt tgtaagagct gcgcaacctt ccagaagtct gtaaaccctc
     1021 aatcccttct cttaccggag aggggatttt tattcatata acaaaacata tagcttgcca
     1081 tatttaaatt taaggaattg ttatgggaaa taatttgtta tcagcaaaag cgacactccc
     1141 tgtttatgat cgtaataacc tggccccaag aattgttcat ttaggctttg gtgcatttca
     1201 ccgtgcgcat cagggcgtgt atgccgatat tcttgctaca gaacatttca gtgactgggg
     1261 atattatgag gtcaacttaa tcggcggcga acagcaaatt gccgatttac aacagcaaga
     1321 taatctttat accgttgcgg aaatgtcggc cgatgcgtgg acggctcgcg tcgttggcgt
     1381 cgttaaaaaa gccttgcacg tacagatgga tggcttagaa accgtgttgg ctgcgatgtg
     1441 tgaaccgcaa atcgcgattg tctctctgac aatcaccgaa aaagggtatt tccactctcc
     1501 ggcgaccgga cagttaatgc tcgagcaccc gatggtcgct gccgacgtgc aaaatcccca
     1561 ccagccgaaa acagcaacag gggtgattgt tgaggcgctg gctcgccgta aagcggcagg
     1621 acttcccgca tttaccgtca tgtcatgtga caacatgcca gaaaacggtc atgttatgcg
     1681 tgacgttgtc acttcctacg cgcaagccgt tgatgtaaaa ctggcacaat ggatcgaaga
     1741 taacgtgact ttcccatcaa caatggtgga ccgtattgtg cccgcagtga cagaggatac
     1801 gctggcgaaa atcgaacaac ttaccggtgt gcgcgatcct gctggcgttg cctgtgaacc
     1861 tttccgccag tgggttatcg aagataactt tgtagccgga cgtccggaat gggaaaaagc
     1921 gggagccgaa ctggttagtg atgtgctgcc ttatgaagag atgaagttgc gcatgctcaa
     1981 cggcagtcat tcattcctgg catatctggg ttatcttgct ggatatcagc acattaatga
     2041 ctgtatggaa gatgaacatt atcgtcatgc ggcgtatggc atgatgttgc aggaacaagc
     2101 gccgacgctg aaagtgcagg gcgttgattt gcaagattac gctaaccgat taattgcacg
     2161 ctatagcaac ccggcgttac gtcatcgaac ctggcagatt gcgatggatg gcagccagaa
     2221 attgccacag cggatgttgg attctgttcg ctggcatctg gcgcatgaca gcaagttcga
     2281 tctgctggcg ctgggcgtcg cgggttggat gcgttatgtc ggtggtgttg atgaacaggg
     2341 aaatccgata gaaatcagtg acccactgtt acctgttatt cagaaggctg tacaaagtag
     2401 tgccgaaggg aaagcgcgcg tccagtcatt gctggcgatt aaggcaatct ttggtgatga
     2461 tttgccagac aatagcttgt ttactgcaaa agtgacggaa gcgtacttgt ctttattagc
     2521 gcatggcgcg aaagcgaccg tggcgaaata ttccgtgaag taaaaaaagc aatgccgcca
     2581 tcggcggcat tcacacactt agttcatgcc gagacgaatc aggtccagag gtgccagttt
     2641 ctcttcacaa ccttcaacaa ctacggtttt tccgcggcgg atttgctcag cggtcagacc
     2701 ttcagtatca atcgacagga tcttgccagt gtgaccggtg ccgctaacca taacacggct
     2761 gccagtggtg attgcgttac ggttacgatc gtaggtcgtc ataggtgttt tctcctttct
     2821 gatttacagt cgaagcccac gtaacgggcg caatataaat actcctgtcg agtgaaatat
     2881 tttttgtttt tgatcaagct cacatctttt ttgatgcatg catgagccgt aaggcggggg
     2941 gaaaagcctc tcatcccgga tgggatgaga gtaaatgaat tactcttcgc taaaccagtc
     3001 gctgttttcc tggcggatct ggcgcacgga ttcgctgatt tcctgcaaat gttgcgtcat
     3061 tgcacgttct accgcatcgc catcacgttt ttgcagggca gagaaaatat caagatgctg
     3121 gcgtaacagc atttctggtg gagaaacatg gtcgaaactc atatagcgca cgcgatcaac
     3181 ggtcgctttc aggttctcaa tggtatccca cgccagttga cagtcggcaa tctgcgtcag
     3241 gagttgatgg aagttgtcat caagttcaaa aaaatcatcc agttgcttgc gctcaatggc
     3301 aatgcgttgc tggtgaagat tttgttccag ttggtagcac tggctttcgg taatcatgct
     3361 cgccgcccga cgcgccaccg cgcactcaat ggcctgacgg ataaaactgc cgttgcgcac
     3421 ctgggccatg gaaattttgt tgacgtagct gccacgctgc ggacgaattt gaatcaggcc
     3481 gttttccgcc agtttaataa aggcttcacg aaccggctgg cgtgacacat tgaaacgaac
     3541 agaaacttct ttttccgaca acggtgtgcc tggtgcaatc aggcaatgga caatgtcgcg
     3601 acgaagaata cgataaatct gctgattgac aggctgtgtg ggattaagtt gcgtttcgac
     3661 ggtcattcgt tcttgcttgt gagtgagtta actgcatgag tctaccactt ttttgtgaca
     3721 agcaataacc cggcagttac gccgggtata aaaattactg acggtggaca ttcagtccgg
     3781 catagctttg ggtaaccggc atcatttcca gggtattgat attgacgtga gcaggcagcg
     3841 ttgacaccca ccagacggct tcgctgacat cttctggcgt caatgcaacg gtattttgat
     3901 aggttttttc cgctttaccg tcatcgcctt taaagcggac attggaaaac tcggtgccac
     3961 ccaccagacc cggttcgatg tcggtgacgc gcaccgccgt accatgcaga tccgtacgca
     4021 gattcaggct aaactgacga acaaacgctt tcgtcgcacc gtaaacgtta ccaccggcat
     4081 acggccagct gcctgccgtt gagccaatgt taataatatg accatgatta cgttcaacca
     4141 tacccggtaa gacggcgcgc gtcatatata ctaggccttt gttgttggta tcaatcatcg
     4201 tttcccagtc ttcaacgctg gctttatgcg caggctccat gcccaacgcc aggccggcat
     4261 tatttaccag gatatcaata ttgctccact cggcagggag cgatgccagc atctcttcaa
     4321 tagcggcgcg gttgcgaacg tccagttggg cgatatacag attatctccc agttcgtctg
     4381 ttaactcctg caaccgctcc tggcgacggc cagtggcgat aactttatgc ccttgttgaa
     4441 taaaacgacg agtaatgcat tcaccaaaac ctgccgttgc tccagttact aaaacgatca
     4501 tctcactgtt cctcaacgct tttgtgtgtc aataacatag cacgtgatgc ttaagagcgg
     4561 gtaatactgg tttgcaagat tgcactccgt tatcagtaag cctacgctat ggaacacctc
     4621 gcgttcagga gaagaaaatg acaacaatga atcctttcct tgtgcaaagc acactgccgt
     4681 atctggctcc tcattttgat caaattgcca atcatcacta tcgcccggca ttcgatgagg
     4741 gaatgcaaca aaagcgggca gaaattgctg ccatcgcgct taacccgcaa acgcctgatt
     4801 tcaacaatac tattctggca ctggaacaaa gcggagaatt aattacccgc gttaccagcg
     4861 tcttttttgc gatgactgcg gcgcatacca atgatgaatt acagcgtctt gatgaacagt
     4921 tttccgctga actggcggaa ctggctaatg atatctatct gaacggtgaa ttattcgcgc
     4981 gggtagatgc tgtctggcag cgccgtgaat ccctggggct tgatagtgaa tccatccgcc
     5041 tggtggaggt tattcatcaa cgttttgtcc ttgccggagc caaacttgca caagctgata
     5101 aagcaaaatt aaaagtactg aatacagaag ctgcgaccct gaccagccag tttaaccagc
     5161 ggttacttgc agcaaataaa tccggcggtc tggttgtgaa cgatatcgcg cagctggcag
     5221 gaatgagtga gcaagagatt gcgctggcgg cagaggcggc tcgcgagaaa ggtctggata
     5281 acaaatggct gattccgctg ctgaatacca cccaacaacc ggcgcttgcc gaaatgcgcg
     5341 atcgtgcgac gcgtgaaaaa ctgtttattg cgggctggac gcgagcggaa aaaaatgatg
     5401 ccaatgatac ccgcgctatc attcaacgtc tggtagagat tcgcgcacag caggcgaaac
     5461 tgcttggttt tcctcattat gccgcatgga aaatcgccga tcagatggca aaaacgccag
     5521 aagcagcact caactttatg cgggaaattg ttccagcggc gcgtcaacgt gcgagcgatg
     5581 aattagcctc catacaggcg gttatcgata agcagcaggg cgggtttagc gcgcagccgt
     5641 gggactgggc attttatgcg gaacaggtac ggcgggagaa atttgatctt gatgaggcgc
     5701 agctcaagcc atattttgaa ttaaacacgg tgttaaatga aggtgtattc tggaccgcga
     5761 atcagctctt cggtattaag tttgtcgaac gttttgatat tccggtctac catcctgacg
     5821 ttcgtgtgtg ggaaattttt gatcataatg gcgtgggact ggcgttattt tacggtgatt
     5881 tcttcgcccg tgattcaaaa agcggcggtg catggatggg caattttgtt gagcaatcaa
     5941 cgcttaatga aacgcatccg gtaatttata acgtctgcaa ttatcagaaa cccgctgccg
     6001 gtgagcctgc gttgttactc tgggatgatg taataacctt attccatgaa tttggtcata
     6061 cgctgcacgg cctttttgcc cgccagcgtt atgccacgct ttccggcacc aacacgccgc
     6121 gtgattttgt cgaatttccg tcgcaaatca acgaacactg ggcaacgcat ccgcaggtat
     6181 tcgctcgcta cgcccggcat tatcagagcg gggcagcaat gcctgacgaa ctgcaacaga
     6241 aaatgcgtaa tgccagcctg ttcaacaaag ggtatgagat gagcgaactg cttagcgccg
     6301 cacttctcga tatgcgctgg cattgcctgg aagaaaacga agcaatgcag gatgtcgatg
     6361 attttgaatt gcgggcgctg gtggcggaaa atatggatct tcctgctata ccgccacgct
     6421 atcgcagcag ttatttcgcc catatttttg gtggcggata tgctgcaggt tattacgctt
     6481 atctgtggac gcaaatgttg gccgatgatg gttaccagtg gtttgttgag cagggcggat
     6541 taacgcgtga aaatgggcag cgttttcgcg aggcgatcct ttccagaggt aacagcgaag
     6601 atctggaacg cctgcatcga caatggcgtg gtaaggctcc tcagattatg ccgatgctgc
     6661 aacatcgtgg cttgaacata taaacaccac caatggctgg cctggagata tcgccaggcc
     6721 agcctggtta tagcagtaat tgcagcagct gggcgagggc aaacctcacc gctaaagcta
     6781 ataccgtttc gcagtcgcca gcaaaatgca taaccgcagt gtagttctgg cctttaatat
     6841 gccacgcgaa ccagacggta cccgctggcg taccatcttc accgccctcc ggtccgccgt
     6901 agccggtaat ggcaatactg acatcagcat ccgcacgctc tatggcacct gttgccattt
     6961 ctgctgccac tttctcactc accgcagaat atcgttcaag agactgctgg cttacgctga
     7021 ggattttcat ctttgcctga tcggtgaaag taacaaagcc tgcaccgtaa aatttgggtg
     7081 tatcttcagc tgcacacagg gcgctagcca acttaccgcc ggtgcatgat tcggcggtag
     7141 ttaagcgtaa ttgatccgcg ataagtcttt gactcaacgc ggatgtcagg ttttcaatag
     7201 tgtcggtatc tgcgagttgt acaattttat ctttattaat gttcactgtt ttttgtccac
     7261 gctattatat gtaaaggtga tttgcttttt ctgtatgctc gtccaatata ataattttca
     7321 aacatataaa tccgattatt ctgaaaacat gtttaagact cttcaatctt cttaacttta
     7381 gtgagtcaaa ttctctgaat atttgacagc tagtgggctg attttaaatg ccttataaca
     7441 attggcagta ttcttatttg gcaattataa aaatagcaac cagacttaat aagtcaaacc
     7501 aaacgcaatt aaggagttag tatgaaattt cagtccattg tattagcaag ttttcttgtc
     7561 atgccttatg cgttggcaga tgaccagggc ggtttaaaac aggatgcagc gccaccgccg
     7621 cctcatgcaa tagaagatgg ctatcgcgga accgatgatg caaaaaaaat gaccgttgat
     7681 ttcgcaaaaa acatgcacga tggcgcttcg gtttcactac gcggtaattt gatttctcac
     7741 aaagg
//
LOCUS       DAZOYF010000177         7723 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZOYF010000177 DAZOYF010000000
VERSION     DAZOYF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.178"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..832)
                     /locus_tag="ACHUOF_003592"
     CDS             complement(<1..832)
                     /locus_tag="ACHUOF_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0612616.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     E"
     gene            1014..2438
                     /gene="sbcB"
                     /locus_tag="ACHUOF_003593"
     CDS             1014..2438
                     /gene="sbcB"
                     /locus_tag="ACHUOF_003593"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR0612617.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2481..2708)
                     /gene="tsuB"
                     /locus_tag="ACHUOF_003594"
     CDS             complement(2481..2708)
                     /gene="tsuB"
                     /locus_tag="ACHUOF_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR0612618.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2722..3780)
                     /gene="tsuA"
                     /locus_tag="ACHUOF_003595"
     CDS             complement(2722..3780)
                     /gene="tsuA"
                     /locus_tag="ACHUOF_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR0612619.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="ACHUOF_003596"
     CDS             complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="ACHUOF_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR0612620.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="ACHUOF_003597"
     CDS             complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="ACHUOF_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR0612621.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5530..6513)
                     /locus_tag="ACHUOF_003598"
     CDS             complement(5530..6513)
                     /locus_tag="ACHUOF_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0612622.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6559..7383)
                     /locus_tag="ACHUOF_003599"
     CDS             complement(6559..7383)
                     /locus_tag="ACHUOF_003599"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0612623.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="ACHUOF_003600"
     CDS             complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="ACHUOF_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR0612624.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2068 a         2013 c         1812 g         1830 t
ORIGIN      
        1 gttcgaagat tggacggcgg ataacattac ccatcttgct atgctctttc ggtacggtcc
       61 atacgttgtc cagcaggtca aattctgtct ttgttgccag cctaagctct gagagcctcg
      121 ccccccacag cataagcatc tgatgaagga gcttatttga cgtagacgca cggcttcttt
      181 caatagcaag ccaaatctta gccagttcgt gatacgacag tacccgatcc cctacctcag
      241 cgcgggagcc gaagtccctt ggctggatgc tcataattgc gcaactatct atcaactgac
      301 gccgcatgca ccaactaatt gctgatctta gttgacttag cacctgcctt gctcggcgtg
      361 gattatcttt ttcttcttcg gtaagcaggt caacccattg cttaaccgtg atagaagatg
      421 ccggacgatt agggaaggcg tcatgcatgc gcttcataac cgttgatcga taaagtgcct
      481 gggtcttttc tctgagagtt gtagagacgt agttgtcgaa ccagtagtcg agacactggg
      541 cgaccgtcat ggagttctcc accttctctt caaaataggt gcgtggatcc gttcctgaga
      601 aatagagctt tcgcaagtca gcagtgatct gtctggcatc cttcaaagac agggatgggt
      661 atcgaccaag cccaagtcga ttaggcttgc catgccagcg atagcggtac tggaactgga
      721 tgaccccctt cggtgaaatt cgtacgctga ggccatcggc atcagccact tcttgtgggc
      781 ccgaatatgg tttaccataa atagtacgca gttttgtgtc gcttatagcc ataaataata
      841 tttggtacgc tcagaaataa tagtttggca cacatctggt acacaatatc acatggacaa
      901 cagcgcacag taaacaacta tatttgacgt tcgtagacat actcaggtga aaaaagggtt
      961 gcttttcgag ttataacaga cagttaatta actacagtgg acaatgctag acaatgacag
     1021 acacagataa acaacctacc ttcctctttc acgattacga aacctttggc acgcaccccg
     1081 cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga tgacgaattc aatgtcatcg
     1141 gcgaacccga agtcttttac tgcaagcccg cggatgacta tttaccccgg cctggagcag
     1201 tattaattac cggtattacc ccgcaggagg cacgggcgaa aggagaaaac gaagccgcgt
     1261 ttgccgcccg tattcactcg ctttttaccg taccgaagac ctgtattctg ggctacaaca
     1321 atgtgcgttt cgacgacgaa gtcacacgca acgtttttta tcgtaatttc tacgatcctt
     1381 acgcctggag ctggcagcat gataactcgc gctgggattt actggatgtt atgcgtgcct
     1441 gttatgccct gcgcccggaa ggaataaact ggcctgaaaa tgatgacggt ctaccgagct
     1501 ttcgccttga gcatttaacc aaagcgaatg gtattgaaca tagcaacgcc cacgatgcga
     1561 tggctgatgt gtacgccact attgcgatgg cgaaactggt aaaaacgcgt cagccacgcc
     1621 tgtttgatta tctctttacc catcgtaata aacacaaact gatggcgttg attgatgttc
     1681 cgcagatgaa acccctggct cacgtttccg gaatgtttgg ggcatggcgt ggcaatacca
     1741 gctgggtggc accgctggcg tggcatcctg aaaatcgcaa tgccgtaatt atggcggatt
     1801 tggcaggaga catttcgcca ttactggagc tggatatcga cacattgcgc gagcgtttat
     1861 ataccgcaaa agccgatctt ggcgataacg ccgccgttcc ggttaagctg gtgcatatca
     1921 ataaatgtcc ggtactggcc caggcgaata cgctacgccc ggaagatgcc gaccgactgg
     1981 gaattaatcg tcagcattgc ctcgataacc taaaaattct gcgtgaaaat ccgcaagtgc
     2041 gtgaaaaagt ggtagcgata ttcgcggaag ccgaaccgtt tacaccttca gataatgtgg
     2101 atgcacagct ttataatggc tttttcagtg acgccgatcg tgcagcaatg aaaattgtgc
     2161 tggaaaccga gccgcgtaat ttaccggcac tggatatcac ttttgttgat aaacgaattg
     2221 aaaagttgtt gttcaattat cgggcacgca acttcccggg gacgctggat tatgccgagc
     2281 agcaacgctg gctggagcac cgccaccagg tcttcacgcc agagtttttg cagggttatg
     2341 ctgatgaatt gcagatgctg gtacaacaat atgccgatga caaagagaaa gtggcgctgt
     2401 taaaagcact ttggcagtac gcggaagaga tcgtctaatt tgaagccgcc gccgctggta
     2461 cggcggagga caatgttgag tcaggctttt tgaacggtga tgctccacgc tgcatcgcca
     2521 atctgctgat aatcggtgat ggcatgtcct tcttctgccg cccactgtgg gattgcttca
     2581 gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc cgctggccat ttctgccagt
     2641 gctgcttttg cttctatgag tggaaacgga cagacctgcg tcaccacatc cagcttttta
     2701 atcgccatct ttacacctta cttaatttgc cgcagcagtt gccagtcgcg ccttacgctg
     2761 cggtcgaaca tacacaagcc aggacgcagt ccagactccg agaatcataa atacaaggcc
     2821 aatccagccc tgccaggtca tcattgcagt cataaccagt ccattaccga tagagcaacc
     2881 acctgcaata ctggcaccga agcccatcag tacaccacct aacccgctac gtaatgttgt
     2941 ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct ttggctgcaa taaatgcctc
     3001 cacgaagatc cctaacacca ggaaaacgcc ccagttaatg tatttcacgt cacccgcgac
     3061 cagaaattga agaatattgg ccgttgggga agtgactcct aacccaaaca tgcgtccggt
     3121 tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg atgagtacag cagtgacaaa
     3181 gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg gcggtaaggt
     3241 cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg ttatcaccag
     3301 caaaacggta accaacggcc acacagacaa attaaaagtc tcagcaatag agttatgttc
     3361 agtagtgtag tgctgcaagg tttgatttaa accactggcg tgtggagaac gcatcaccgc
     3421 actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct cacccgcgcg
     3481 ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga aaagataccc
     3541 acctataaca gtaccaagcc acgggaacgc cccggcttcg taagtcagta ggcccgcctg
     3601 aattaacgca aaaaccccca cgctttgtgc cgaaattgca atcagcaagg cgtaaaacat
     3661 gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa aacgcccacg
     3721 ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca ttgaaaacat
     3781 aaattaatta accagatgaa tgttaatggg gaaatttttc atgactggtg gaatgcagac
     3841 caataaccaa attctctaaa ttagaacaaa tggttattaa tgaggatttc gataaaaaag
     3901 aataaaaaaa ccggagccaa atgttcattc gactccggtt tattattaga aaagatgatt
     3961 atgctacgtc ttcgtactgc ggaaccggat tacggaagct cttggtgacg caagccaggt
     4021 aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct tccaggttaa
     4081 cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac tggaagtgat
     4141 ctttcagcgt cttgttacgc ttctcacgga tccagaactg cgagatgacc gacaggttaa
     4201 caaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt accaggtcga
     4261 agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc
     4321 atttcgggtg tacataaccg aagaagcttt tcgggaacac gccgtcacga cccattacgt
     4381 acatcagacg cgctacgcct gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg
     4441 agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg atttcaggct
     4501 gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc aggaagtaag
     4561 ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca
     4621 cgcgctctgc atctttggtt tcttccgaca gattgctgat gccgtcaaag ccggtaaagg
     4681 agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg ccagaccaga
     4741 acggacgcgt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca taaacaacca
     4801 tgcccagaat caccgcgatc agcactacct gcaacacgac gattacagtg ttgacgttcg
     4861 ctacggattt cagactacgc aggttaaagg cggtcataaa ggccaccagc gccaccacaa
     4921 acatccacga tggaatggaa ggcaccagag cttcaaaata gattttcgcc agcagaatgt
     4981 tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata aagccgacag
     5041 tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa
     5101 ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac gcataggctg
     5161 tcggcacatg accatccgtg aggcctgaaa cgataccaaa tgtatcaaac agcgtcatcg
     5221 gctgcatata ggctagaccc atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt
     5281 ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt tattctcctt tacggtgata
     5341 accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac aacaactgtc
     5401 gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa tgattggttt
     5461 gaagcaaaaa ataaccgacg ctaattaaac gtcggttttt aatcattttt tgacagcggc
     5521 gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca taataatttt
     5581 tctttgaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga tcgcactatt
     5641 tgctaaaatt tcatctcgcc accagccgct ggcaagccct gcggtctgtt cattccagcc
     5701 aatccagaca ggttcaaaga gagtttgggc gaccactttc ttctcgatta acgcgccact
     5761 atcgagaaaa cgttgcgcca gataccgtgg taaatagccg cagccaaggc cgctaatttg
     5821 cagctccagt ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt cgagaagttg
     5881 tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc gatatcgttt
     5941 aatgatacga cggtttaacg gttcttcttc ctgggctaac ggatgatgag gcgcgaccgc
     6001 aaagacttgt tcaagatcgc ccaaccgcga gaaaccaaac tcactggaag atggtggctc
     6061 atgcatcgcg ccaacgataa tatccgctcg cccctgagtc agggcatccc aggaaccacc
     6121 gagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa aagcttcaat
     6181 gagaggcgca agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca gctcgttttc
     6241 ccagccttca tgcagtttga tcgcctgctt ttccagttct cgcacggtat gcagaacttc
     6301 ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt gtccgctacg
     6361 atcaagcaat tgaatattta ggtcgctttc cagcttgtga acggtgtaac tcagagcgga
     6421 cggcgtcttg tagagtttgg ctgacgccgc cgcaaaactg ccctcttttt ctatggcatc
     6481 gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt gcatgcggcg
     6541 tcctgctggc gtgatcgttt actccaacgg cattaccagc ggatcgggat actggtattc
     6601 aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc cgctgtccag
     6661 actatttctg aactgcggag gttgcagccc cagtaaacgg gccatctgcg gatagaaaac
     6721 attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt taggtgcctg
     6781 taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa caccatgttc
     6841 accatcaggg gcggtttttc cggcaaagaa gcgtcccgga tgacgtcccg gtccgaccag
     6901 gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag
     6961 ttcttctaat actcgtccac tgttggttac cggattacgc ggggtagttt ctttaaccgt
     7021 gccttgcgcg tcgccataga cagatgttga gctggtaaaa ataattcggg gaatacgatg
     7081 ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat cgccggggcc
     7141 gctacgacgt gccggaagcg taatgaccag cgcatcggca tccataaggg catccagatc
     7201 gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa tgccactcat
     7261 tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc
     7321 tgaaagtgac atcgccagcg gcatacctaa ccaccctaac ccgacaattg cgaccttttt
     7381 catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta tcaaaactga
     7441 caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca atgagcagtg
     7501 aatcatcagt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg gaccagaaac
     7561 ctgacaaatt atgtttcagg ggttctggtt tccccttacc ttcaaatggt gttctgcggg
     7621 tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc tgccagtaca
     7681 gataatcatc ccatgattcc tcagaccaga ttagtttcac tca
//
LOCUS       DAZOYF010000178         7594 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZOYF010000178 DAZOYF010000000
VERSION     DAZOYF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.179"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(117..1588)
                     /gene="yeeO"
                     /locus_tag="ACHUOF_003601"
                     /pseudo
     CDS             complement(117..1588)
                     /gene="yeeO"
                     /locus_tag="ACHUOF_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            1749..1824
                     /locus_tag="ACHUOF_003602"
     tRNA            1749..1824
                     /locus_tag="ACHUOF_003602"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1782..1784,aa:Asn,seq:gtt)
     gene            complement(1862..2812)
                     /gene="cbl"
                     /locus_tag="ACHUOF_003603"
     CDS             complement(1862..2812)
                     /gene="cbl"
                     /locus_tag="ACHUOF_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR0612625.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2914..3831)
                     /gene="nac"
                     /locus_tag="ACHUOF_003604"
     CDS             complement(2914..3831)
                     /gene="nac"
                     /locus_tag="ACHUOF_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR0612626.1"
                     /translation="MNFSRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4159..4234
                     /locus_tag="ACHUOF_003605"
     tRNA            4159..4234
                     /locus_tag="ACHUOF_003605"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4192..4194,aa:Asn,seq:gtt)
     gene            complement(4290..5225)
                     /gene="ldtA"
                     /locus_tag="ACHUOF_003606"
     CDS             complement(4290..5225)
                     /gene="ldtA"
                     /locus_tag="ACHUOF_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0612627.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5287..6366)
                     /gene="cobT"
                     /locus_tag="ACHUOF_003607"
     CDS             complement(5287..6366)
                     /gene="cobT"
                     /locus_tag="ACHUOF_003607"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR0612628.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6378..7121)
                     /gene="cobS"
                     /locus_tag="ACHUOF_003608"
     CDS             complement(6378..7121)
                     /gene="cobS"
                     /locus_tag="ACHUOF_003608"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR0612629.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7118..>7594)
                     /gene="cobU"
                     /locus_tag="ACHUOF_003609"
     CDS             complement(7118..>7594)
                     /gene="cobU"
                     /locus_tag="ACHUOF_003609"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR0612630.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2098 a         2053 c         1648 g         1795 t
ORIGIN      
        1 actgaactac agaggaatcg tgagaacgag gcgaatatta gcgatgccca cccacaatgt
       61 caaagcctgt tttttaaatt tgaaatcgtt tgctgaaata atctgcattt tgtcgtttat
      121 tccgacacaa ctggcttttt ttcacacttt tgcggctcgg atcgagggta tttccacagc
      181 caacgtccag taaccattcg ccagtaaaac agcacggccc gcacagccca gtcggcaaac
      241 attcccatcc agacaccaac cacaccccag ccaagcatga ttccctgcac ataaccgact
      301 acaacccggc aaccccacat gctcaacata gaaacccaca tggcgtaacg ggcatcacga
      361 gcacccttaa atccagcggg tagcacccat gaggcggacc aaataggcat aaataaagca
      421 tttagctaaa tcagaatcac aacgacgtgt ttaacctgtg gatcctgggt gtaaaacgat
      481 gccataaccc cggcaaaggg agccgttagc caggcgatgg ccgttaatcc aagagtggac
      541 agccagaaca catgccgcaa ctgaatctct gcttgcgcta tctgccctac ccccaacctt
      601 cggcctgtaa tgatcgtaga agcagaaccg agcgcacttc cgggtaagtt gataagagcc
      661 gcaattgaga acgcgataaa atttccggca ataacgctgg tccccatccc ggcaacgaac
      721 atttgggtta ataaccgacc actggtaaat aacactgatt cgacactcgc tggaatacca
      781 atccccatga cttcccagat aatgctaaaa ttcagcggtt taaaatagct ctttaacgaa
      841 atccttagcg caggattaaa accaatcgcc agcacccaca aaattgcaac tgcgccaata
      901 taacgagaaa tggttaaacc caacccagcc ccgaccagga gaaaaggccg taaatcaata
      961 tgccgctaat aataatatta agaatattca ggctaccgtt aatcagtagc ggtattttcg
     1021 tattccctgc accacgaagt gccccgctac caataagagt gatggcagcg gctggataac
     1081 tgagtacggt cagctccaga taagtcaacg ccagtgcttt aacttctgtc gtggcatcac
     1141 ccgcgacgaa atcaataatt tgttcgccaa aatgatgaat tagcgttgcc aacagtacgg
     1201 caaacaacgt catgatcacc aatgactgcc gcgtcgccac cctcgctcgt cgtcgatccc
     1261 gcttaccgag actaaatgct acaacgacag tagtaccaag atcgatagct gcaaaaaaag
     1321 ccataatgac catattgaag ctgtccgcca accccacgcc ggccatcgca tcttttccca
     1381 gccaactgac cagaaaagtg ctcaggaccc ccatcaacag gacacaggcg ttctccatga
     1441 agataggaac agcaagcggg gttatctcgc gctagaacaa cactttgtag ctcttgcgtt
     1501 tagagtgcca gcgagtgccg tgaacaacct ggcgtaaagc agaggagata ttcaaagccg
     1561 accttaattg cagaaagtga aaccacattt caaatgatga gggagaatca gcaaagctgc
     1621 aaagattttc gccaacaaat tgtctgcaaa tgcaacaaac tgttgataga aacggcaaac
     1681 agttgggaaa tttaaaaatc gggtttgaca aaatattttt cgccgttaag atgtgcctca
     1741 acagcgattc ctctgtagtt cagtcggtag aacggcggac tgttaatccg tatgtcactg
     1801 gttcgagtcc agtcagagga gccaaatttc tgaaaagccc gcttttatag cgggcttttt
     1861 gctatatctg ataatcaatt tcctcttcac tgttttccat cacctggcgc ttgatatcct
     1921 caactgacag tcctgcatta caaagttcca gaaagcgcca aacatagtta cgctgaagtt
     1981 gtcctcgctt cagtcccaac cagacagtat tagcatcgaa aagatgccgc gtatccaggc
     2041 ggattaaatt ctcttcctct tgttcgccgc tggattgttc ggcaactaat ccgatcccaa
     2101 gcccaagagc aacataggtt ttaatgacat cagaatcctg tgcacttaat acaatatctg
     2161 ccggcaaacc tttgcgggta aatgcgtcat cagtgcgtga gcgccccgta attccctgtc
     2221 ggtaagtaat taacggccac ttcgctattg attccagcgt caatggtgta atttgcgtta
     2281 agggatgatc gagtggaaca agcaaactat ggtgccaaca aaaccatggg aaggcgacga
     2341 gctgcgggtc attactcaaa cgctcgctgg cgataccaat atcagcttcg ccattttgca
     2401 acaacgtcgc aatttcctgt ggcgaaccct ggattagctc gagccgaacc tccgggaaaa
     2461 gttcgcgaaa agctttaatg acctctggca agctataacg tgcctgagta tgcgtcgttg
     2521 caatagtgag aacaccagac gtatcgttgg taaacaggtc tgcaagccga cgaacattac
     2581 tggcttcatt cagaatacgt tctgcaatga ccagtaatgc tttgcccggt tcagtcatcc
     2641 ccagcagtcg cttacctcgt cgaacaaata tttcgatgcc aaattcatcc tccagttccc
     2701 gaatatgacg gctaacgcct gactgtgagg taaaaagcat attcgcaacc tctgtcaggt
     2761 tgtaatcctg acgtgcagcc tcgcggatta tctttagttg ttggaaattc acggtaaact
     2821 ccgggcagtt cagatatccc gttattgtta aagtctaatg cccggcataa caaataataa
     2881 aaacccgcat cttattccat cccgatataa cgcttagctc accaattgcc actgcctttt
     2941 ttccatcact ggagaactaa tcactgacat taacaactct ttcactgcct gtgcctgtgg
     3001 tgacaagttt gctctggcgg gtaaatttaa ggacaaagag agactcattg aaggagtggt
     3061 aatgcgtgac atccacccat ttactgcgcc acataacgaa cgcgcagccg attcaggtaa
     3121 tactgcaacg cccatgccgc tggcaatcgc tgcggtaagc gtggcaatag actcaatttc
     3181 accaatgact tttgccgtga gccgccgtag ggaaaaagcc tcatcaacac gaagtctaac
     3241 agcgctgtaa tcactgggga gaaaaaggtt catttgcgca atagcattaa catcaacgtt
     3301 ttgccccggg caatcttgag ttcctaccag gaaaagatct tctttcagca aagcctgact
     3361 ggatacccca gccacagggg aatgctcata aatcaccgcc atatcgagtt ggtgatttat
     3421 cagtttttcg ttaagcactg caccactatt ttcatgaaga tagataacga cctccgggaa
     3481 ttcagcgcga accgcctgta ataagggcat ggtgatggat gacgcagcgg ttcctggcgc
     3541 aaagccaatc gagacttgcc ccgataatga ctgaccaacg ttatgcaccg ccagttgggc
     3601 ctgctcacac tgacgtaaaa tggcccgtgc atgggtatag agaatttttc cggcgtctgt
     3661 tggcgtaacg ccccgttttg tgcgaatcaa aagctgttga tttaactcac cttccagtat
     3721 ggcaacctgt tggctgagcg ctggttgtgc gatatgcaat acttcagcag cctgggtcag
     3781 gctaccaata tctacaattt ttacgaagta tttcaggcgg ctgaagttca tgttgcctcc
     3841 ggtttttaag aatcggccca agtgccgcca ttacttacaa ccagtatgca agatgcctgc
     3901 cagttctatt ttggcgttga tgcgcaagct aaccaactgt caaataagag attatgatag
     3961 attcgtcatt ggcccttttt atcagcagtc gcattcacct gccctataaa aggagggtat
     4021 gcaccacgat ggttcattac ccaataagat tgaaagctca ccactttgtt gaaattggca
     4081 gcaaacaaac aaaaaaaatg catttcaccc tttgacatca ccatgcactg ccattaatat
     4141 gcgccccgtt cacacgattc ctctgtagtt cagtcggtag aacggcggac tgttaatccg
     4201 tatgtcactg gttcgagtcc agtcagagga gccaaattca aaaaagcctg ctttcgagca
     4261 ggctttttac tttctaatta ccaacgctct taaaacatct gtcttgaacc agaactaatt
     4321 tgcacaggca ttcccgatcg atgttgcaac gcagcatttg cgcgatttac atcaacttct
     4381 tgcccgttga taaacgcccg caaagatggg gttaccggca atggcacttt tcggtcagac
     4441 tcatattctg cacgattgcg cgacagcggc tcatgaactt ccagccactt tgaaccatcc
     4501 ggttccgttg tgtatttcac tggctgatca ataatttgta cacgagtgcc tacaggaaca
     4561 ttatcaaaca gatatttgat atcgtcatta cgcagacgaa tacagccctg acttacacgg
     4621 agcccaatac caaaattggc attggtaccg tggatggcat acaacctgcc aatataaatc
     4681 gcgtacagcc ccatggggtt atcgggcccc gcaggaacaa atgcgggcaa actctcccct
     4741 cgtttcgcat attcgcgccg gatgttcggc gttggcgtcc aggttggtgc ttcttgttta
     4801 cgttcaacgg tagtcaccca gttacgcggg gtttctcgcc cagcctggcc gataccaata
     4861 ggaaagactt ccactgtatt actgtctggc ggatagtaat aaagacgcat ttcagcgacg
     4921 ttaaccacaa tccctttacg aacagtggcg ggcaaaatca gttgctgcgg aatggtgagt
     4981 tgtgagccag gcttcggcaa aaaaacatca gcgcccggat tcgcttccag catattactt
     5041 aacccttgcc cgtattgtgc ggcaaaagtc tccagcggct gagtattgtg atcaggaaca
     5101 gttacagtaa gcgactgccc cactaaacgg ctaccctctg gaggtaatgg ataagttacc
     5161 gccaggctat tttggctggc aaaaagcaga gcaaatgagc aaagaatatt tacacgacgc
     5221 atcatgtccc tttcctaagt cgcgaatact atccgataag tatagctttt atcagacttt
     5281 tcgtttttag ctgttcaaat cagaagtcgt attccccggt agaacaatat tactggcagc
     5341 aagttcgccc atgttgttgt atatcgcaca agcagcttcg atgatggaca tcgccagagc
     5401 tgcaccactc ccctcaccta aacgcatatc catattgaga taaggctcca gccccaaatg
     5461 cgagagcgct atacgcgcgc ctttttctgc cgacaagtga gaaggaatga gatacggttt
     5521 gattgcagga gacatctggc aggctgcgag cgcagcagca taagaaagaa atccatccag
     5581 caacacgggt aaaccgcagg aagcagcacc taacatcact ccagctattc cgaccaaatc
     5641 aaatccaccc acttttgcca ggacatcaac accatcctga ggatttggtt gattcaacgt
     5701 aatcgcccga cgcacaacat caattttatt agccagttta tctgtcggca ggtttgcgcc
     5761 aatcccaacc acttcttcag gatcccggcc agtgattgtg ctgactattg ccgctgccgg
     5821 tgtcgtgttt gccatcccca gttcacctac accaaacagc gtgacaccgt ttttcgccag
     5881 ctcccgcgta taacatatga cgtccaaaag caacttttca gcctgacggc gactcattgc
     5941 cggagctgaa gcaatattgc cgctacctcg tgcgacacgc atgttgataa gcccggggat
     6001 aggctcagca gtatcaatac caacatcaat tacgtggacg ttagcgcccg cttgtgctgc
     6061 cagcacacac acgccggttg ttccacgggt catattttca gcctgtatgg ctgtcacttc
     6121 ttttggagaa atagcgaccc cttcctccca gacgccgtga tcggcacaca taaccagtac
     6181 cgcttttttg cccacatgcg gtatgccatt cacccccggc attcctgcca gttgtatggc
     6241 aagcgcctcc agctttccta ggctaccaac aggtttgaat aacccgtcaa catgccgttg
     6301 tgcacgcgac atagcggcag aattgatggc tgggatcgta ttcagtaaat cggcaattat
     6361 ttgcatgtca cgtttcttta tagcagtgcc agcaggaaga ccagttcacc aagctctatg
     6421 gctgctccca gcgtatcgcc cgtttgcccg cccagcgtac gtttgagaag ctgaccgagg
     6481 atgaaaatcg ccaccatcgt caccaccata gcagccacac catgcatacc cagcaataat
     6541 acggcagcaa aaatagcggc taagccgagt gtgacgcagg tttgtcgccc atcaattttg
     6601 ccaatgaaca cattgccaag cccttcttcc cgcgcataac gatgacgata catcaataat
     6661 gcggcagtcc cgcggctgac cgcacatgct gccgccagcg atgcaaggat cggttcgcca
     6721 cgcagagcca actcgcttaa caccagaatc tttgccagta ccacaaaaat caatgccaga
     6781 ccgccgtggg tgcctaaacg gctatcacgc atgatttcca acatgcgatc gcgactacgt
     6841 gcagaaaata cgccatcaca ggtatcagcg aggccgtcca gatggaattc cccggtcatc
     6901 agcgccagca caagtacgct aaacagtgcc gccagtggtg cgccacacca tgcctgcagc
     6961 accatgaaga ccagcccgct aatcgcgcca agcaataatc caatcaaagg aaaagtaata
     7021 ataccgcgag aataatgttc gaaatccagt ccctgggacc agcgacgcgg tacaggcaga
     7081 cgcgtaataa atgagagcat cgcccaaaat aatttgctca tttgattttt actccaatac
     7141 ccgaaaccac cagccatact tcatttgccg cagcggccaa ctgctgattt acccgcccgg
     7201 caatatcacg aaaatgtcgt gccagacgac tctccggcac aatccccatt cccacttcgt
     7261 tagtcactaa tacaaccttt gcggggcaac gttggcaggc agcaatcaac gactgaatct
     7321 cagcattaat cgcctgttcc atcgcctgat aatcccattc atcagggtct ttatcgccgc
     7381 cataatcaaa caacagatta gtcaccattg tggtaacgca ttccagcaac acagcctcat
     7441 tcgggttaat gtctgcatga attaattcat caagatgttg ccagcgctcc actgtgcgcc
     7501 agtgctccgg gcggctttgc cgatgatgtt ctatccgtgc agccatctca tcatcaagga
     7561 tttgcgaggt agcgatatac agaacctgtg aaga
//
LOCUS       DAZOYF010000179         7575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZOYF010000179 DAZOYF010000000
VERSION     DAZOYF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.180"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..972
                     /locus_tag="ACHUOF_003610"
     CDS             <1..972
                     /locus_tag="ACHUOF_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0612631.1"
                     /translation="TRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSH
                     LSLKGIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNF
                     AQIKMADGTLNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYV
                     NLFKADLSFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICT
                     GSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENI
                     PIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(1126..2775)
                     /locus_tag="ACHUOF_003611"
     CDS             complement(1126..2775)
                     /locus_tag="ACHUOF_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR0612632.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2926..4275)
                     /gene="ghxP"
                     /locus_tag="ACHUOF_003612"
                     /gene_synonym="yjcD"
     CDS             complement(2926..4275)
                     /gene="ghxP"
                     /locus_tag="ACHUOF_003612"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR0612633.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4981..5445)
                     /gene="soxR"
                     /locus_tag="ACHUOF_003613"
     CDS             complement(4981..5445)
                     /gene="soxR"
                     /locus_tag="ACHUOF_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR0612634.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5531..5854
                     /gene="soxS"
                     /locus_tag="ACHUOF_003614"
     CDS             5531..5854
                     /gene="soxS"
                     /locus_tag="ACHUOF_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR0612635.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5857..7434)
                     /gene="pdeC"
                     /locus_tag="ACHUOF_003615"
                     /pseudo
     CDS             complement(5857..7434)
                     /gene="pdeC"
                     /locus_tag="ACHUOF_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
BASE COUNT         2112 a         1954 c         1729 g         1780 t
ORIGIN      
        1 acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt atattttata
       61 aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa tggtggttac
      121 gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt atccaattcg
      181 aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat gtataaaacg
      241 aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct cgatgactca
      301 aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc tatgcatgtt
      361 caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct tgactattca
      421 aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc gccatatgtt
      481 aatttattta aagccgacct tagtttctct aaactcgatt taattaactt tgaacatgct
      541 gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt aattgatagc
      601 aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg taccggctct
      661 aatatggcta atgttaattt taataatgcc aatttaagca actgccattt caactgttct
      721 attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt cgatgaggct
      781 agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc cattgatagt
      841 gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca tactggcatg
      901 tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga tattatgcaa
      961 cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa gagagataac
     1021 ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt gcaaaagatt
     1081 gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact gattttcctc
     1141 aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg tttcgttgct
     1201 gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca atgccgccag
     1261 acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt tacgtccatc
     1321 ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca actggttatc
     1381 gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg ccacaatcgc
     1441 cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg aatggtccgc
     1501 gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca gcgagaagag
     1561 aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt taccatccgg
     1621 caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca ccccagcgaa
     1681 agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct tcttcttcag
     1741 gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca gcatcagcgc
     1801 cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag ggtcgatttc
     1861 tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca gcaggaacac
     1921 catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca ggcgcattgc
     1981 cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg caaccgcagc
     2041 gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg gcagcaagaa
     2101 cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga atcgcagcga
     2161 gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg ctactttcat
     2221 aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca ctgccacggc
     2281 aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct gcaaaatgcc
     2341 catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca cagcatcggt
     2401 cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg gcaccaccca
     2461 gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg cgaggccgaa
     2521 aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat cagcgaacag
     2581 caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat gcaaaccaaa
     2641 cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg ggatctgaaa
     2701 gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta tcagtatggt
     2761 gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag aaagacgtta
     2821 tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc ggagaaaata
     2881 aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga tagcccagcc
     2941 acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt tcaacttgcg
     3001 ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca tgatgcctgt
     3061 tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca tcgcatcaac
     3121 aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa cgtacatcag
     3181 cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga acagaatcag
     3241 caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg ccgccgtacc
     3301 cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag agaaaacgct
     3361 gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat ctttatccag
     3421 caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata ctgccgtcat
     3481 caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa tgtccaggct
     3541 gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa cgccggagaa
     3601 atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg tcagcagaat
     3661 gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg ccagtgacat
     3721 aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa gcgggttttt
     3781 aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga taccgatccc
     3841 cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga tacccgtggc
     3901 agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga caggtacgct
     3961 aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga tggcgcaacc
     4021 aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc cggcaaccag
     4081 acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca tacctggaac
     4141 gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca cttcctgacg
     4201 gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc cggtacgcgc
     4261 tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa gatgcgtggc
     4321 ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc aatttggtgc
     4381 gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa aaaaagcaaa
     4441 cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt ttctctttta
     4501 ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg atgtgcaaga
     4561 ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt ctgagatgac
     4621 aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg gtgacataca
     4681 attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt gtgttgacgt
     4741 cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc ggaaaacaaa
     4801 ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa agagctcgat
     4861 cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag caccagtgca
     4921 aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc ttgtggcgct
     4981 ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta agcggtcgcc
     5041 cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac atccgtccag
     5101 ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc gccattggga
     5161 cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca cgccaaacgc
     5221 ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa ttgcgacata
     5281 tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac tagtaatcaa
     5341 ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt tcgccacttc
     5401 gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc gctttacctc
     5461 aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa cactgaaaag
     5521 aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga ttgacgtgca
     5581 tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt caaagtggta
     5641 cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca ttcgccaacg
     5701 ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt ttgatatcgc
     5761 aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc ggcagtttga
     5821 tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg ttaactcccg
     5881 cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct tcgcaaaaaa
     5941 ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct cctcagtttc
     6001 gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga tgtgttccgc
     6061 aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat cgattttcaa
     6121 aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac caaaatcatc
     6181 aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc attttttcga
     6241 catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt
     6301 gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca
     6361 ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga tcgcggaaga
     6421 cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa
     6481 taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac aacgcttcag
     6541 cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat
     6601 gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta
     6661 gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat
     6721 gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc
     6781 gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt aaatccttca
     6841 aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt
     6901 tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc
     6961 agaagagagg gttgataacc gccacataat ttccccgctg catataggtc attttatagc
     7021 cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat
     7081 cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt
     7141 tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     7201 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     7261 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     7321 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     7381 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     7441 caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta ccgacgtctt
     7501 tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat acctttttaa
     7561 tcgtcacctg gatga
//
LOCUS       DAZOYF010000180         7559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZOYF010000180 DAZOYF010000000
VERSION     DAZOYF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.181"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(323..1723)
                     /gene="asnS"
                     /locus_tag="ACHUOF_003616"
     CDS             complement(323..1723)
                     /gene="asnS"
                     /locus_tag="ACHUOF_003616"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR0612636.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1892..3094)
                     /gene="pncB"
                     /locus_tag="ACHUOF_003617"
     CDS             complement(1892..3094)
                     /gene="pncB"
                     /locus_tag="ACHUOF_003617"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR0612637.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3360..5975
                     /gene="pepN"
                     /locus_tag="ACHUOF_003618"
     CDS             3360..5975
                     /gene="pepN"
                     /locus_tag="ACHUOF_003618"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR0612638.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(6182..6949)
                     /gene="ssuB"
                     /locus_tag="ACHUOF_003619"
     CDS             complement(6182..6949)
                     /gene="ssuB"
                     /locus_tag="ACHUOF_003619"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0612639.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(6946..>7559)
                     /gene="ssuC"
                     /locus_tag="ACHUOF_003620"
     CDS             complement(6946..>7559)
                     /gene="ssuC"
                     /locus_tag="ACHUOF_003620"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR0612640.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         1925 a         2001 c         1909 g         1724 t
ORIGIN      
        1 tatttatatg atttacttat atttaaatta actaaacgga aattttgttt ctgatggaaa
       61 ctttatcgac ctggcacaaa atcctctttc agacatccag aatgccaaaa aatagtatga
      121 aaattgtgct atcagctaag aaaaaactaa tccgcaataa gagtacgggt tattttttgt
      181 gcgtaaatgt tgcaatgtaa tattccgtaa caggatgatc gttatggcgg gggtgcaaat
      241 agattgcttg acttaacaaa cagataactt gacagaaagg ataaaaaaga gccagcaggc
      301 gctggctctt tgaggaagaa aatcagaagc tggcgttgcg cggagtacgt gggaacggaa
      361 tcacatcacg cacgttttgc acgccagtta cgtaagcaat caggcgttca aaaccaagac
      421 cgaaacctga atgcggaaca gtaccgtagc gacgcagatc gcgataccac cagtaatctt
      481 ctttattcag gcccatttcc agcatacgct cgtccagaac gtccagacgt tcttcacgct
      541 gggagccacc aatgatctca ccgatgcccg gagccagaac gtccatagcc gcaacggttt
      601 taccgtcttc gttgaggcgc atatagaacg ctttaatatc tttcggatag tttttaacca
      661 ctaccggtgc tttaaagtgt tcttccgcca gataacgctc atgctcagaa gagagatcga
      721 cgccccagta aaccgggttt tcaaacttcc tgccgcagtt ttcgagaatg gtcactgcgt
      781 cggtgtaatc cacctgcgcg aaatcggctt caatgaagcg ttccagacgt gaaacggcat
      841 ctttatctac gcgttcagcg aagaatttca tgtcgtctgc gcgttcttcg agaaccgctt
      901 tgaagacata tttcagcatg gcttcagcca gacccgcgat atcgttcagg ttagcaaacg
      961 ccacttccgg ctccagcatc cagaattccg ccaggtgacg gctggtgttg gagttttcag
     1021 cacggaaagt cgggccgaag gtataaattt tggataatgc gcaagcgtag gtttcgccgt
     1081 tcaactggcc agatacggtc aggaaagact ctttaccaaa gaagtctttg tcgaaatcca
     1141 ctttgccctg atcgttacgc ggcaggtttt ccagatccag cgtagaaacg cggaacattt
     1201 cgcctgcacc ttcggtatca gatgcggtaa tcagtggcgt tgaaacccag aagaatccct
     1261 gctcgttaaa gaagcgatgc agcgcctgcg ccagcgtatg gcgaacgcgc gcgacggcac
     1321 caatcaggtt tgtgcgcgga cgcaggtgag cgacttcacg cagatactca atgctgtggc
     1381 gttttgccgc catcgggtaa gtgtctggat cttcaaccca accagcaact tcaaccttgc
     1441 tggcctgaat ttcaaattgt tgcccctggc ccggcgatgc cacgacttta cccgtcacaa
     1501 tgaccgagca gccggtggtc agacgcagga cgtcttcatt gtaattgggc agagaattat
     1561 tgatgacagc ctgtacagga tcaaagcagg aaccgtcata aacggcgagg aaggagatgc
     1621 cagcttttga atctcggcgg gtacgtaccc atccgcgtac ggtgacttcg ctgtcaacgg
     1681 ctacgcggcc ctggagtacg tcggctacag gcacaacgct cataatattc tctctgttaa
     1741 tagtcggaaa aaataaacac ttgtccaccc gtaaatgggg gggataccta tgttacctgg
     1801 catctgcaat cagacaagca gaattcgcaa atgcagcgaa agatttcgga attaaaggta
     1861 aaaaagggag ccgattagct ccctgatgat attaactggc ttttttaata tgcggaaggt
     1921 cgaacgcttt gcgcagcgcc cgaacaaacg ctttatcatg gcagatagtt ttgccagggc
     1981 tgtcagaaag tttcgccacc ggtttaccgt tacactctac caacttaatc acaatattca
     2041 ggggttttac ctgggggata tcgcaggtca ggcgagtccc aataccaaaa cttaattgca
     2101 cgcgggaaga gaagtggcgg tatagctcaa ccgctttgcg taaatccaga ttgtcagaga
     2161 aaaccagcgt tttactctgt ggatcaattc ccagcttttc ataatgtgca atggcttttt
     2221 caccccattc aaccgggtcg ccagagtcat gacgcaggcc ctgataccga ctagcgaact
     2281 cgacaccgaa atcacgcagg aaagcatcca tagtgatgca gtcggttaat gcaatgccaa
     2341 gttggtcggg atactcttcc agccaggcag caagtgcagc tcgctggctg ttggctagat
     2401 ccgggctgat ttgctgatgt gcctggaacc attcgtgtgc ctgtgttccc atcggcgtga
     2461 gggaaagccg acgcgccaga tcgtagttgc tggtgcccac gaaccaggat tcctgttgca
     2521 gacgcttaac gatggtttct tgtacttcgc gagaaaaacg gcgacgggtg ccaaaatcca
     2581 tcagatggaa gcgcgacata tcaagaccgg cggttaacgc cgagaagtcg actaatttgc
     2641 tttccagcgt gtcgagggct tgcgcaacgt cggcctgcgg tgagcgatag cgatgtacca
     2701 tttcactgat aaccgccagc aaaggaactt cccagaggat gacttcacgc cacggaccgc
     2761 ttaagcgaat atccagcttg ccattatcgt tggacacggt gacttgttcc gggttaaagc
     2821 ggaactcgcg taaccagtta agatagtcgg ccttaaagaa aggcagggca gagagccact
     2881 gatattcatc atcctgcagg cgcaggtgct gcatcgcctg aatctgttca cgaatagcat
     2941 cggcataaat acccagcaga tcgtcacctc ggcaacgaaa ctccgccgcg acatgcacat
     3001 cgtaatagtg atgaaacacg gcttgctgca tatgcaactt ataagcatct gtatccagca
     3061 acgagtgcag aacaggagaa gcgaattgtg tcataggtgc gcagtagcgt cctcgtacag
     3121 gagcgtttag tacaataaac atcttcagga actgctggag tataccttgt ttcgcaattt
     3181 attgaacccc gatcacacca tatgccacct ttctggtcga tggcattacg cggtgtatgt
     3241 tataaaaatg tagcaataaa ggcgtttgta cctgaaaaga tgaagattct gcatagcgcg
     3301 atttacgcaa caggaataga ctgaacacca gactctataa aagatgctaa aggttattta
     3361 tgactcaaca gccacaagcc aaataccgtc acgattatcg tgcgccggat taccagatta
     3421 ctgatattga cttgaccttt gacctcgacg cgcaaaagac ggtcgttacc gcggtcagcc
     3481 aggctgtccg tcatggtgca tcagatgctc cgcttcgtct caacggcgaa gacctcaaac
     3541 tggtttctgt tcatattaat gatgagccgt ggaccgcctg gaaagaagaa gaagagggcg
     3601 cactggtcat cagtaatttg ccggagcgtt ttacgcttaa gatcattaat gaaataagcc
     3661 tggcggcgaa tacggcgctg gaagggcttt atcagtcagg cgatgcgctt tgcacccagt
     3721 gtgaagccga aggtttccgc catattacgt attatctcga ccgcccggac gtgctggcgc
     3781 gttttaccac caaaattatt gccgataaaa tcaaatatcc cttcctgctt tccaatggta
     3841 accgcgttgc gcaaggcgaa ctcgaaaacg gacgccattg ggtacagtgg caggacccgt
     3901 tcccgaaacc gtgctacctg tttgcgctgg tggcaggcga ctttgatgta ctgcgcgata
     3961 cctttaccac gcgttctggt cgcgaagtag cactggagct gtacgtcgat cgcggcaacc
     4021 ttgatcgcgc gccgtgggcg atgacctcgc tgaaaaactc catgaaatgg gatgaagaac
     4081 gctttggcct ggagtatgac ctcgacatct atatgatcgt cgcggtggat ttcttcaata
     4141 tgggcgcaat ggagaataag ggtctgaata tctttaactc caaatatgtg ctggcccgca
     4201 ccgacaccgc caccgacaaa gattacctcg atattgaacg cgttatcggc catgaatatt
     4261 tccataactg gaccggtaac cgagtgacct gccgcgactg gttccagctc agcctgaaag
     4321 aaggtttaac cgtcttccgc gatcaggaat tcagctctga ccttggttcc cgcgcagtta
     4381 accgcatcaa taatgtgcgc accatgcgcg gattgcagtt tgcggaagac gccagcccga
     4441 tggcgcaccc aatccgcccg gacatggtca ttgagatgaa caacttctac accctgaccg
     4501 tttacgagaa gggcgcggaa gtgatccgta tgatccacac cctgctgggc gaagaaaact
     4561 tccagaaagg gatgcaactc tatttcgagc gtcatgatgg tagcgcagcg acctgtgacg
     4621 actttgtgca ggcgatggaa gatgcgtcga atgttgatct ctcccatttc cgccgttggt
     4681 acagccagtc cggtacgccg gttgtgaccg tcaaagacga ctacaatccg gaaactgagc
     4741 agtacaccct gaccatcagc cagcgcacgc cagccacgcc ggatcaggca gaaaaacagc
     4801 cgctgcatat tccgtttgcc atcgaattgt atgacaacga aggtaaagtg atcccgttgc
     4861 agaaaggcgg tcatccggtg aattccgtgc tgaacgtcac ccaggcggaa cagacctttg
     4921 tttttgataa tgtctacttc cagccggtgc ctgcgctgct gtgcgaattc tctgcgccag
     4981 tgaaactgga atataagtgg agcgatcagc aactgacctt cctgatgcgt catgcgcgta
     5041 atggtttctc ccgttgggat gcggcgcaaa gtttgctggc aacctacatc aagctgaacg
     5101 tcgcccgtca tcagcaaggg cagccgctgt ctctgccggt acatgtggct gacgctttcc
     5161 gcgcggtgct gctcgatgag aagattgatc cggcgctggc ggcagaaatc ctgacgctgc
     5221 cttctgtcaa tgaaatggct gaactgttcg atatcataga cccgattgct attgccgaag
     5281 tacgcgaagc actcactcgt actctggcga ctgaactggc ggatgagctg ctggctattt
     5341 acaacgcgaa ttaccagagc gagtaccgtg ttgagcatga agatattgca aaacgcactc
     5401 tgcgtaatgc ctgcctgcgc ttccttgctt ttggtgaaac gcatctggct gacgtgctgg
     5461 tgagcaagca ataccatgaa gcaaacaata tgaccgatgc gctggcagcg ctttctgcgg
     5521 cggttgctgc acagctgcct tgccgtgacg cgctgatgca ggagtacgac gataagtggc
     5581 atcaggacgg tctggtgatg gataaatggt ttatcctgca agccaccagc ccggcggcga
     5641 atgtgctgga gacggtgcgt ggtctgttgc agcatcgctc atttaccatg agcaacccga
     5701 accgtattcg ttcgttgatt ggtgcgtttg cgggcagcaa cccggcagcg ttccatgccg
     5761 aagatggcag cggttaccag ttcctggtgg aaatgcttac cgacctcaac agccgtaacc
     5821 cgcaggtagc ttcacgtctg attgaaccgc tgattcgcct gaaacgttat gatgccaaac
     5881 gtcaggagaa aatgcgcgcg gcgctggaac agttgaaagg gctggaaaat ctctctggcg
     5941 atctttacga gaagataact aaagcactgg cttgatactt ttgagccaaa atgcctgatg
     6001 cgacgcttga cgcgtcttat caggcctaca actccgttga aattgctggt tttgtaggct
     6061 ggataaggcg ttcacgccgc atccggcagc cgtgcccaac tgcctgatgc gatgcttgac
     6121 gtgtcttatc aggcctacaa gtccgttgaa aatgctggtt ttgtgcgcca catccggcat
     6181 attaaccatg acgccgaatc agttgttcag aatgcccacg ccgcattacc cgttgcaata
     6241 cttccgcttc cagctccgct aatcgcaccg accctaatcg acgcggacgg gggatatcca
     6301 ctgtcaaatc cagactaatt tttccctctt caattaacaa cacccggtca gccattgcta
     6361 ccgcttcgct cacatcatgc gtcaccaaca gtacggtaaa gccgtgctgc tgccaaagtg
     6421 acacaatcaa atcctgcatc tcgagtcgcg ttaaggcgtc cagtgccccg agcggttcat
     6481 caagcaacaa taaacccggt cgatgaatca acgctcttgc cagcgccaca cgctgtttct
     6541 gcccgccaga aagtgcggca ggccattccc cggcgcgatt ctccagtcct gccgcagcca
     6601 acgcccgacg cgcggcatcg cgccactgac ctttaaggcc taacccaacg ttatcaatca
     6661 ccgatttcca cggcagcaga cgcgcatcct gaaacatcat tcgcgtatca tcctgaattt
     6721 cagccagcgg tgtggtgccc gctaacacat cgcctgcggt tggcgtttcc agaccggcca
     6781 gcaggtgcag cagggtactt ttaccaccac cgctgcggcc caccaccgcc acaaactgac
     6841 ctgccggaat atgtaaatcc agttggttca ggacgatatt ttccgcgtaa tgtttgctta
     6901 ctgcattgag caacaatggt gtgccctggt tcagacgagc agtattcata tcgtggcctc
     6961 cttcaaatga taagccgggt tccagcgcaa ccagaggcgc tctaacagct gcgcgctgac
     7021 gtcagcaagt ttgccgagca gggcgtaaag aataatagcg actaccacca cgtctgtttg
     7081 caaaaactct cgcgcattca tcgccagata accaatgccg gaattggcag aaatggtttc
     7141 ggcaacaatc agcgtcagcc acatcaggcc caacgcaaaa cgcacgccga ccataattga
     7201 gggcagggca tcgggcagga tcacatggat aaacagcggt atgccggata atccatagct
     7261 acgcgccatc tccaccagcc cgcgatcgat attacggatg ccatgccagg tgttgatata
     7321 aatggggaac agcgtaccga gcgccaccag aaagattttc gcggattcat caatgccaaa
     7381 ccacaaaatc accagcggga tcagcgccag atgcggcacg ttgcgcaaca tctgaattga
     7441 ggtatccagc agcctttctc cccagcgcga cagtccgcta atcagtccca gaatcaggcc
     7501 cagcgatccg ccaattgaaa agccaatcag cgcccgccag gaactgatcg ccagatgct
//
LOCUS       DAZOYF010000181         7537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZOYF010000181 DAZOYF010000000
VERSION     DAZOYF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.182"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..1160
                     /gene="rbsA"
                     /locus_tag="ACHUOF_003621"
                     /pseudo
     CDS             1..1160
                     /gene="rbsA"
                     /locus_tag="ACHUOF_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1165..2130
                     /gene="rbsC"
                     /locus_tag="ACHUOF_003622"
     CDS             1165..2130
                     /gene="rbsC"
                     /locus_tag="ACHUOF_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR0612641.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2155..3045
                     /gene="rbsB"
                     /locus_tag="ACHUOF_003623"
                     /pseudo
     CDS             2155..3045
                     /gene="rbsB"
                     /locus_tag="ACHUOF_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3171..4100
                     /gene="rbsK"
                     /locus_tag="ACHUOF_003624"
     CDS             3171..4100
                     /gene="rbsK"
                     /locus_tag="ACHUOF_003624"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR0612642.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4104..5096
                     /gene="rbsR"
                     /locus_tag="ACHUOF_003625"
     CDS             4104..5096
                     /gene="rbsR"
                     /locus_tag="ACHUOF_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR0612643.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="ACHUOF_003626"
     CDS             complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="ACHUOF_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR0612644.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6512..7204)
                     /locus_tag="ACHUOF_003627"
     CDS             complement(6512..7204)
                     /locus_tag="ACHUOF_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR0612645.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1918 a         1826 c         1986 g         1807 t
ORIGIN      
        1 aaaaccatgt atgtcgaagc ggataaattg ctggctaaac ttaacctgcg ctttaaaagc
       61 gacaagttag tgggcgatct ttccatcggt gaccagcaaa tggttgaaat cgccaaagtg
      121 ctgagctttg agtcgaaagt catcattatg gatgaaccga ccgatgcgct gaccgatacc
      181 gaaaccgaat ccctgttccg cgtcatccgc gagctgaaat cgcaaggcca ctgtattgtc
      241 tatatctccc accgcatgaa agaaatcttc gagatttgcg atgacgttac cgtttttcgt
      301 gatgggcaat ttattgctga gcgcgaagtg gcatcactga ccgaagattc gctgattgag
      361 atgatggtgg gccgcaagct ggaagatcaa tatccacacc tggacaaagc gccaggagat
      421 atccgcctga aagtcgataa tctctgcgga cctggcgtta acgatgtctc ttttacttta
      481 cgcaaaggcg aaattcttgg cgtctctggt ttgatgggcg caggtcgtac cgaactgatg
      541 aaagtgctct acggcgcact gccgcgcacc agcggttacg tcaccctgga tgggcatgaa
      601 gtcgttaccc gttcaccgca ggatggcctg gcaaacggca ttgtgtatat ctccgaagac
      661 cgtaaacgtg acggtttagt gctgggcatg tcagtaaaag agaacatgtc gctgacagcg
      721 ctgcgctact tcagccgcgc tggcggcagt ttgaagcatg ccgatgaaca gcaggctgtg
      781 agtgatttca ttcgtctgtt taatgtgaaa acgccgtcga tggaacaggc aattggtctg
      841 ctttccggtg gcaatcagca aaaagtggcg attgcccgcg gtctgatgac acccccaaag
      901 tattgatcct tgatgagcct acccgtggcg tagatgtcgg cgcgaaaaaa gagatctatc
      961 aactgattaa ccagttcaaa gccgatggct tgagtatcat tctggtgtca tcggagatgc
     1021 cagaagtatt aggcatgagc gatcgcatca tcgtcatgca tgaagggcat ctcagcgggg
     1081 aatttactcg tgagcaggcc acccaggaag tgttaatggc tgtcgctgtg ggcaagctta
     1141 atcgcgtgaa tcaggagtaa aaaaatgata acccagactg tctctggtcg ccgttatttc
     1201 acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc tgctggtgct gatcgcgatt
     1261 gtctcgacgt taagcccgaa ctttttcacc atcaataact tattcaatat tctccagcaa
     1321 acttcggtga acgccattat ggcggtcggg atgacgctgg tgatcctgac gtcgggcata
     1381 gacttgtcgg taggttctct gttggcgcta accggcgcag ttgctgcatc tatcgtcggc
     1441 attgaagtca atgcgctggt ggctgtcgct gctgctctca cgttaggtgc cgcaattggt
     1501 gcggtaaccg gggtgattgt agcgaaaggt cgcgtccagg cgtttatcgc tacgctggtt
     1561 atgatgcttt tactgcgcgg cgtgaccatg gtttatacca acggtagccc agtgaatacc
     1621 ggctttactg agaacgccga tctgtttggc tggtttggta ttggtcgtcc gctgggcgta
     1681 ccgacgccag tctggatcat ggggattgtc ttcctcgcgg cctggtacat gctgcatcac
     1741 acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg aagcggcaac gcgtctttct
     1801 ggtatcaacg tcaataaaat caaaatcatc gtctattctc tttgtggtct gctggcatcg
     1861 ctggccggga tcattgaagt ggcgcgtctc tcctctgcac aacccacggc ggggactggc
     1921 tatgagctgg atgctattgc tgcggtggtt ctgggcggta cgagtctggc gggcggaaaa
     1981 ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg gcttccttaa taatggattg
     2041 aatttgttag gtgtttcctc ctattaccag atgatcgtca aagcggtggt gattttgctg
     2101 gcggtgctgg tagacaacaa aaagcagtaa taacgactac aggacatctt gaatatgaac
     2161 atgaaaaaac tggctaccct ggtttccgct gttgcgctaa gcgccaccgt cagtgcgaat
     2221 gcgatggcaa aagacaccat cgcgctggtg gtctccacgc ttaacaaccc gttctttgta
     2281 tcgctgaaag atggcgcgca gaaagaggcg gataaacttg gctataacct ggtggtgctg
     2341 gactcccaga acaacccggc gaaagagctg gcgaacgtgc aggacttaac cgttcgcggc
     2401 accaaaattc tgctgattaa cccgaccgac tccgacgcag tgggtaatgc tgtgaagatg
     2461 gctaactagg cgaacattcc ggttatcact cttgaccgcc aggcaacgaa aggtgaagtg
     2521 gtgagccaca ttgcatctga taacgtactg ggcggcaaaa tcgccggtga ttacatcgcg
     2581 aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag gcattgccgg tacatccgca
     2641 gcccgtgaac gtggcgaagg cttccagctg gccgttgctg ctcacaagtt taatgttctt
     2701 gccagccagc cagcagattt tgatcgcacc aaaggtttga acgtaatgca gaacctgttg
     2761 accgctcatc cggatgttca ggctgtattc gcgcagaatg atgaaatggc gctgggcgcg
     2821 ctgcgcgcac tgcaaactgc cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca
     2881 ccggatggcg aaaaagcggt gaatgatggc aaactagcag cgactatcgc tcagctaccc
     2941 gatcagattg gcgcgaaagg cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt
     3001 caggctaagt atccggttga tctgaaactg gttgttaagc agtagtttta atcaggttgt
     3061 atgacctgat ggtgacataa atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc
     3121 agggcacgcg ccaccctaac acggtggcgc attttatgga catcccgaat atgcaaaacg
     3181 caggcagcct cgttgttctt ggcagcatta atgctgacca cattcttaat cttcaatctt
     3241 ttcctactcc aggcgaaacc gtaaccggta accactatca ggttgcattt ggcggcaaag
     3301 gcgcgaatca ggctgtggct gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta
     3361 ctggcgatga cagcattggc gagagcgttc gccagcagct cgccactgat aacatcgata
     3421 ttactccggt cagcgtgatc aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg
     3481 gcgaaggtga gaatgtcatc ggtattcatg ccggcgctaa tgctgctctt tccccatcgc
     3541 tggtggaagc gcaacgtgag cgtattgcca acgcgtcagc gttattaatg cagctggaat
     3601 caccactcga aagtgtgatg gcagcggcga aaatcgccca tcaaaataag actatcgttg
     3661 cgcttaaccc ggctccggct cgcgaacttc ctgacgaact gctggcgctg gtggacatta
     3721 ttacgccaaa cgaaacggaa gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag
     3781 atgcagcgaa ggcggcgcag gtactgcatg aaaagggtat ccgtactgta ctgattactt
     3841 taggaagtcg tggtgtatgg gctagcgtga atggtgaagg tcagcgcgtt cctggattcc
     3901 gggtgcaggc tgtcgatacc attgctgccg gagatacctt taacggcgcg ttaatcacgg
     3961 cgttgctgga agaaaaacca ttgccagagg cgattcggtt tgcccatgct gccgctgcga
     4021 ttgccgtaac acgtaaaggc gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat
     4081 ttttagacag gcagaggtga cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg
     4141 tttctacctc aacagtttct cacgttatca ataaagatcg cttcgtcagt gaagcgatta
     4201 ccgccaaagt tgaagcggcg attaaagaac tcaattacgc gccatcagct ctggcgcgta
     4261 gcctcaaact caatcaaaca cataccattg gcatgttgat cactgccagt accaatcctt
     4321 tctattcaga actggtgcgt ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg
     4381 tcctttgcaa taccgaaggc gatgaacagc ggatgaatcg caatctggaa acgctgatgc
     4441 aaaaacgcgt tgatggctta ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa
     4501 tcatgcaacg ttatccgaca gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg
     4561 acagcgatct tattcaggat aactcgttgc tgggcggaga cttagcaacg caatatctga
     4621 tcgataaagg tcatacccgt atcgcctgta ttaccggtcc gctggataaa actccggcgc
     4681 gcctgcggtt ggaaggttat cgggcggcga tgaaacgtgc gggtctcaac attcctgatg
     4741 gctatgaagt cactggtgat tttgaattta acggcgggtt tgacgccatg cgccaactgt
     4801 tatcacatcc gctgcgtcct caggccgtct ttaccggaaa tgacgctatg gctgttggcg
     4861 tttaccaggc gttatatcag gcagagttac aggttccgca ggatatcgcg gtgattggct
     4921 atgacgatat cgaactggca agctttatga cgccaccatt aaccactatc caccaaccga
     4981 aagatgaact gggggagctg gcgattgatg tactcatcca tcggataacc cagccgaccc
     5041 ttcagcaaca acgattacaa cttactccga ttctgatgga acgcggttcg gcatagattt
     5101 acgctgtctt ttgatcaaat tattaccatc ggttgttttc agaagcatga acattgctgc
     5161 tgaagcaaca gtaataatgc ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt
     5221 tgtgccttcc atcccttcat aaatgcgaag gacggccgca cttacagcaa cgcctaaact
     5281 aatcgacagt tgctgcgtga ccgccagaac actgttaccg ctgctggcgt tgtcatcggt
     5341 cagatcggca agtgtgatgg tattcatcgc ggtaaactgc gtcgacatag ccatccctaa
     5401 tataaacaac ggcaagatca gcatccatat cgccattgcc ggtgattgca aagagaactg
     5461 agcgatcatt agcccaataa tcaccgtgat ccccactaat gtatggcgat agcccagacg
     5521 acgtaagact tgggtaacca tcgattttgc aataatggaa cctaacgctg tcggtgccat
     5581 catacagccg gcaataaacg cctgataacc aaatcctacc tgtaacatca atggcataag
     5641 gaacggtaca cagccggtcc ccagacgggt tgcaatattg cctacgatac cgatcgagaa
     5701 agtgcgggtt ttaaataaat ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag
     5761 aatatagaga agcagtaacc cgatgctggt gacaattacc gtcaaggcaa tccagctggc
     5821 gacaatcttt tccccgaata gctctattcc gcttgaaaag agaacaaggc tgaggccaaa
     5881 cagcaaaaag ccagtgatat cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt
     5941 gcgcgcgtaa agaaggcccg caatacctat ggggatatta attaaaaata tccagtgcca
     6001 ggttgcccag gtgaccagca cgccgccaag aacggggcct aaaattggcc ccaccagacc
     6061 cggcatggcg acaaaattca ataccggaag aagttcatta cgaggataag cacgcagtaa
     6121 ggccggccga gcaacaggca tcatcattgc gtcgcctatc ccttgaataa cccggaagac
     6181 aaccagctgt ggtagcgaat tagaaagtgc gcaggccaga gaacccaacg tgaacagact
     6241 cacggcaagg gtaaaaatgc gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg
     6301 aataagcatc gccaccgtca gcgtataact gatgatggct gattgcatcg cgagaggaga
     6361 acgattaagg ctatgagcga ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc
     6421 ctgcatgaag aaggccatcg ccgcgatcca cggcaaaccc gacatactac gcttcttttt
     6481 atcgctcatt caatgtcctg ttatcgggtt atcacttatc aggtgagcgt agcagcgcct
     6541 gacaagcttt aaatgccgcg tcgccatcgc tttggataat cgcatcgaca atcgcctggt
     6601 gcagatccag ctttatcact gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg
     6661 aatggaatag cgaggcgaat gaggtcaaaa acggattggc gctcatttca tagatatgct
     6721 catgccaggc catatcgact tcgatccagc gttcacggcg aaagttctct tttaatgccg
     6781 ccatttcggc cattaacgta ttgagatgcg ccttctgttc cgcggtgcca accgttgctg
     6841 ccagtaggca ggcttgcggc tccagacaga tacgcataac cagaaagtga tcgatgacct
     6901 gatgaaagtt ctcttctgtt atccaccaag taagcaattc ctgatcaaga aaattccagt
     6961 tcgattgtgg catgacccga gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg
     7021 ttaacgtttt gaccgcttcg cgtaccgctg tacgactcac tccaaattgc tcgcccagct
     7081 caatctcacc gggcaaaatg gtgccgggtt catattcacc ttttaagatc cgttgcgcca
     7141 gcttctcagc cagaacatac gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag
     7201 gcattactta tcttcctttt tctttttatt cctccttagt atgccaccag gaagtgtgat
     7261 tacggttgca aaaacggcaa attgcttgtt ttatggcaca ttaacggggt gttttgctga
     7321 aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccggg ataactccct
     7381 ataatacgcc accactgaca cggaacaacg gcaaatacgc cgccgggtca gcggggttct
     7441 cctgagaatt ccgacagaaa aagcaaaaat aaatgattga ctctgtagcg ggaaagcgta
     7501 ttatgcacac cccccgcgcc gctgagaaaa agcgaag
//
LOCUS       DAZOYF010000182         7425 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZOYF010000182 DAZOYF010000000
VERSION     DAZOYF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.183"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="ACHUOF_003628"
     CDS             complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="ACHUOF_003628"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR0612646.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(723..1751)
                     /gene="malI"
                     /locus_tag="ACHUOF_003629"
     CDS             complement(723..1751)
                     /gene="malI"
                     /locus_tag="ACHUOF_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR0612647.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1926..3518
                     /gene="malX"
                     /locus_tag="ACHUOF_003630"
     CDS             1926..3518
                     /gene="malX"
                     /locus_tag="ACHUOF_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR0612648.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3528..4700
                     /gene="malY"
                     /locus_tag="ACHUOF_003631"
     CDS             3528..4700
                     /gene="malY"
                     /locus_tag="ACHUOF_003631"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR0612649.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4804..5805
                     /gene="add"
                     /locus_tag="ACHUOF_003632"
     CDS             4804..5805
                     /gene="add"
                     /locus_tag="ACHUOF_003632"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR0612650.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5841..6881)
                     /locus_tag="ACHUOF_003633"
     CDS             complement(5841..6881)
                     /locus_tag="ACHUOF_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR0612651.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7124..7249
                     /gene="blr"
                     /locus_tag="ACHUOF_003634"
     CDS             7124..7249
                     /gene="blr"
                     /locus_tag="ACHUOF_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR0612652.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
BASE COUNT         1758 a         1933 c         1830 g         1904 t
ORIGIN      
        1 tctggtgtaa taacggattt cagggcatcg gttaatattg cccccggcgc aatgctattc
       61 acccgaatat ttttttcacc caggtcaaat gccatatttc tgaccagatg actggccgca
      121 gctttagatg atgcatagga agtcatgttt atatttttat tttctgccgc catagaagtg
      181 atggtcagaa taacgccacc tccatttttt tccatttctg gcgcaacaag ttgtgacaga
      241 tggaaaaaag aaaacacatt cagttcataa gcacggcgaa aatctgccat tggcatatca
      301 aacggtttag gaccaccgcc accggcgttg ttaaccagga tatcaacttt acccagatta
      361 ctgacggcaa agtctgccag tgcagagagt tcctgttcgg aagtaatatc acaacgccag
      421 gcaaatgcct gaccatccag ttgttgaatt tcgtctacaa catggttagc tgcgtcggcg
      481 ttaatatcac tgaccaccac agatgcgcca gctgtcgcga atgtaatggc gatttcttta
      541 ccaatacctg cacccgcacc tgtgatgatg gcgcattttc cgtcgagtct caggttgtca
      601 gaattaaaca cataaacctc ctgtgaactt cagtaaaaat aaaaggcacc gcctgtgtgc
      661 cacatttttt atcattaacc ttgaggaaag gacactacca agtatagcag ataaagagag
      721 gattatttcg ccgcaatgag ccgggcggga ataataagat tgcgtgaatg cgtctcttca
      781 tgggtgattt tttgcatcat gcgatccgca agtgtgatac caagttcccg cgctggcgtg
      841 ctggcccagg taacaggtat atcatcaagt gtggttggtg tcgcatcggt aaatgccgcc
      901 agcgaaacct gttgctcaaa gtaacgatcg actccgcttt ccccgctttg ccgccctgct
      961 ttcagcaaac caaaccatgc ccccatcgca atagtttcgt tatagcaaac cacggcactg
     1021 atggtcgggt tatgacgtaa aagcgccgtg atagcttccg cggcttgctt ctggctggaa
     1081 gtgcactcca acacccaatc gctgtgaaac ggcaggccaa attttagtag agttgcacaa
     1141 tagcccccca cccgttctgc acgggttaat gaggaacttt gccctcccag ccaggcgatc
     1201 cgctgatgcc cattgcgaat gagatgctcc gtcaacaact gtgcagcctg catgttgtcc
     1261 gggcgaaccg tatcaacatc atcaagataa ctggcacggg aagcgaaaat caccgggata
     1321 gctttttctt ctgccatccg ccgcaggtca tcgctgcttc ctgcagcccc ggcaattacc
     1381 acaccatcga caccctgatt cagtaacagt gaaaaccgct gtgccagctg ctcgccgtct
     1441 ttaccgccgt gaagcaaaaa aaccatccgt ccctgtgctt ccagagcttc cgtcaatccg
     1501 gccgtcaatt cggcgtaaaa cggcgcagat aaatcacgga cgatcaaacc aatgacgccg
     1561 ctttgcccgc cgcgcagcgc cgacgcctgg cgattgcgca caaatcccag ctcttcaatg
     1621 gcggcgttaa cgcgttctcc tgtggcggta gagattcgcc ctttgccatt aagcaccagc
     1681 gaaacggtac ttaccgacac gcccgcagcc agcgcaacat catgaatggt tatttttttg
     1741 gcggtagcca tgcagatgac ctactccctg attatgtgac agataaaacg ttttaccttt
     1801 tatttatctt atacccgcta ttatcgttgc gtaatgtgat ttatgactca ctaaaatttg
     1861 ataaaacgtt ttatcttctc gcgcaattta ctgaatccag attgttctct acgaggagtc
     1921 gttttatgac ggcgaaaaca gcaccgaaag tcacgctgtg ggagttcttc cagcagttag
     1981 gcaaaacctt catgttaccc gtggcattat tgtcgttctg cggcattatg ctcggcattg
     2041 gtagttctct tagcagccat gatgtcataa ccctgatccc ggtcctgggc aaccccgtgt
     2101 tgcaggctat ctttacctgg atgagtaaga ttggctcgtt tgcttttagt ttcctgcctg
     2161 tcatgttctg tatcgccatc ccgctgggcc tggcacgcga aaataaaggc gtagcggcat
     2221 tcgctggctt cgtcggttat gcggtaatga acctcgcggt aaacttctgg ttgaccaata
     2281 aaggcattct gccaaccaca gatgccgccg ttctgaaagc caataacatc cagagcattc
     2341 ttgggatcca gtcgatcgat accgggatcc tcggtgcggt gatcgccggt attatcgtct
     2401 ggatgctgca tgagcgtttc cataatatcc gcctgccgga tgcgctggca ttcttcggcg
     2461 gtacgcgctt cgtaccaatt atctcctcgc tggtgatggg ccttgtcggc ctggtgattc
     2521 cattagtctg gccgattttc gccatgggta ttagcggctt gggccatatg ataaacagcg
     2581 cgggtgattt cggaccgatg ctgtttggta ccggtgaacg tctgctgttg ccgtttggtc
     2641 tgcatcacat tctggtggca ttaattcgct ttaccgacgc aggcggcacg caggaggtct
     2701 gcggtcaaac cgtcagcggc gcactgacca tcttccaggc gcaattgagt tgcccgacca
     2761 ctcacggttt ttctgaaagc gtcacgcgtt tcctttcgca aggtaaaatg cctgcgtttc
     2821 tcggcggtct accaggtgca gcgttagcta tgtatcactg cgcgcgcccg gaaaatcgcc
     2881 ataaaattaa aggtctgctg atttctggcc tgatcgcctg cgtcgttggc ggcactaccg
     2941 aaccgctgga attcctgttc ctgttcgtag cgccagttct gtatgtcatc cacgcgctgt
     3001 taaccggcct cggcttcacc gtcatgtctg tgctcggcgt caccatcggt aataccgacg
     3061 gcaatatcat cgacttcgtg gtgttcggta ttttgcatgg tctgtcaacc aagtggtaca
     3121 tggtgccagt ggtggcggca atctggtttg tcgtttacta cgtcatcttc cgtttcgcta
     3181 tcacccgctt caatctgaaa accccggggc gcgatagcga agttgccagc tcaatcgaaa
     3241 aagccgttgc cggtgcgccg ggtaaatcag gttacaacgt tcctgcaatc ctcgaagcat
     3301 taggcggtgc cgacaatatt gtcagcctcg ataactgcat tacccgtctg cgtttgtctg
     3361 tggaagatat gtcgcttgtt aatgtgcagg cactgaagga caatcgggca attggcgtag
     3421 tacaacttaa tcaacataac ctgcaggttg ttatcgggcc acaagttcag tcagtaaaag
     3481 atgaaatggc cggtctgatg catactgtcc aggcataagg ataagatatg ttcgattttt
     3541 caaaggtcgt ggatcgtcat ggcacatggt gtacacagtg ggattatgtc gctgaccgtt
     3601 tcggcactgc tgacctgtta ccgttcacga tttcagacat ggattttgcc actgccccct
     3661 gcattatcga ggcgctgaat cagcgcctga tgcacggcgt atttggctac agccgctgga
     3721 aaaacgatga gtttctcgcg gctattgccc actggttttc cacccagcat tacaccgcaa
     3781 ttgatacgca gacggtggtg tatggccctt ctgtcatcta tatggtttca gaactgattc
     3841 gtcagtggtc tgaaacaggt gaaggcgtgg tgatccacac gcccgcctat gacgcatttt
     3901 acaaggccat tgaaggtaac cagcgcacag taatgcccgt tgctttagag aagcaggctg
     3961 acggctggtt ttgcgatatg ggcaagctgg aagccgtttt ggcgaaacca gaatgtaaaa
     4021 ttatgctcct gtgtaaccca cagaatccta ccgggaaagt gtggacgtgc gatgagctgg
     4081 agatcatggc tgacctgtgc gagcgtcatg atgtgcgggt tatttccgat gaaatccata
     4141 tggatatggt ctggggcgag cagccgcata ttccctggag taatgtggcg cggggagact
     4201 gggcgttgct gacatcggga tcgaaaagtt tcaatattcc cgcgctgacc ggtgcttacg
     4261 ggattataga aaatagcagc agccgtgatg cctatttatc ggcactgaaa ggacgtgatg
     4321 ggctttcttc cccttcggta ctggcgttaa ctgcccatat cgccgcctat cagcaaggtg
     4381 caccgtggct ggatgcctta cgcgtctatc tgaaagataa cctgacgtat atctcagata
     4441 aaatgaacgc cgcgtttcct gaactcaact ggcagatccc gcaatccact tatctggcct
     4501 ggcttgatct acgtccgttg aatattgacg acaacgcgtt gcaaaaagcg cttatcgagc
     4561 aagaaaaagt cgcgatcatg ccggggtata cctatggtga agaaggtcgt ggttttgtcc
     4621 gtctcaatgc cggctgccca cgttcgaaac tggaaaaagg tgtggctgga ttaataaacg
     4681 ccatccgcgc tgttcgctaa ccccaattgc gcaacgtaaa aaatcgttgc gcaatcgtgg
     4741 atttttaccc tgctttgttt ttataatggt gcgcactttt atatccagaa aaagagtgcg
     4801 accatgattg ataccaccct gccattaact gatatccatc gccaccttga tggcaacatt
     4861 cgtccccaga ccattcttga acttggccgc cagtataata tctcgcttcc tgcacaatcc
     4921 cttgaaacac tgattcccca cgttcaggtc attgccaacg aacccgatct ggtgagcttt
     4981 ctgaccaaac ttgactgggg cgttaaagtt ctcgcctctc ttgatgcctg ccgccgcgtg
     5041 gcatttgaaa acattgaaga tgcagcccgt aacggcctgc gctatgtcga gctgcgtttt
     5101 tcaccaggct acatggcaat ggcacataag ctgcctgtag cgggtgttgt cgaagcggtg
     5161 atcgatggcg tacgtgaagg ttgccgcacc tttggtgtgc aggcgaagct tatcggcatt
     5221 atgagccgaa ccttcggcga agccgcctgt cagcaagagc tggaggcctt tttagcccac
     5281 cgtgaccaga ttaccgcact tgatttagct ggtgatgaac ttggattccc gggaagtctg
     5341 ttcctttctc acttcaaccg cgcgcgtgat gcgggctggc atattaccgt ccatgcaggc
     5401 gaagctgccg ggccggaaag catctggcag gcgattcgtg aactgggtgc ggagcgtatt
     5461 ggacatggcg taaaagccat tgaagatcgg gcgctgatgg attttctcgc cgagcaacaa
     5521 attggtattg aatcctgtct gacctccaat attcagacca gcaccgtagc agagctggct
     5581 gcacatccgc tgaaaacgtt ccttgagcat ggcattcgtg ccagcattaa cactgacgat
     5641 cccggcgtac agggagtgga tatcattcac gaatataccg ttgccgcgcc agctgctggg
     5701 ttatcccgtg agcaaatccg ccaggcacag attaatggtc tggaaatggc tttcctcagc
     5761 gcagaggaaa aacgcgcact gcgagaaaaa gtcgccgcga agtaacaaaa atggatggtg
     5821 caaattgcac catccatttt tcacgcaagg cacaacgtcg cgcgatgttt ggcggattcg
     5881 atgcccagct caatcaactc cattacctgg attgcctggc ttgccggaac cggattttca
     5941 ccatcgccat ttaacgcatc acgaatcgcc gcatagtaag ccggataatt cccaggcacc
     6001 gtcaacagcg tttcttcgac acgttcctca ccttccacgc gggtaagtac gccatcacgc
     6061 atatcgtagc cccagtcttc ctgcggcaga cgctcgccat ttttcagacg ttcttcctgt
     6121 ggatcgaggc catatttcac ataactgcct cgggatccat gcacgatata ccgtgctgac
     6181 tcagcggctg ccagcatggt accgtgtaaa atgactcgcc gctgtggata ggacaagatg
     6241 gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact gtgccaaatc taccgtcatg
     6301 ctgaccggta aaccaaatag cgtaatggcc tgatcaagaa gatgcggtgc taaatcgtac
     6361 cagataccgc tgcctgggcc gccctgttca cgccaacgat cgcgcacctg cggacggaag
     6421 cggtcaaaat gagactcaaa gtaagcaact tcacccagca cgccttccgc gagcaaacct
     6481 tttagcgtca agaaatcgct atcccaacgg cggttatgga atacagacag cacacgcccc
     6541 aggctttttg ccagcgcatc cagctctcgc gcttgtgaca gtgtcacggt aaagggttta
     6601 tcaacgacca catgtttacc cgcctcaagc gccgctttgg ctaacgggaa atgggtatcg
     6661 ttgggtgtag gaatgacaat caggtctatg ttgggatcgt taaacagatg cttcggctca
     6721 gagacaaccg taaccgttgg ccagtcggct tttacttttg tttcatcact gctggagatt
     6781 accgccagtt ccagcccggg cgtgcccgca atcaggggcg catggaaggt tttgctcgca
     6841 taaccatacc caatcaaccc aacacggatg ttgtcgctca tgtcattgcc tctcattgaa
     6901 gtatgatggc tatttgacac tatcctttac ccacgctcaa cagtttaata acctgccagc
     6961 aataagggat gttgtttaac ttaagtcaaa aaaatagcga attttccaac gacaaaagct
     7021 aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa gtaacattta
     7081 ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc gtcttattga
     7141 attaacaggt tggatcgttc ttgtcgtttc agtcattctt cttggcgtgg cgagtcacat
     7201 tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag ctctgcactt
     7261 gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat taaaccagga
     7321 atttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg cgttactctt
     7381 ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattc
//
LOCUS       DAZOYF010000183         7381 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZOYF010000183 DAZOYF010000000
VERSION     DAZOYF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.184"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            126..1523
                     /gene="ulaA"
                     /locus_tag="ACHUOF_003635"
     CDS             126..1523
                     /gene="ulaA"
                     /locus_tag="ACHUOF_003635"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFR0612653.1"
                     /translation="MEILYNIFTVFFNQVMTNALLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1539..1844
                     /gene="ulaB"
                     /locus_tag="ACHUOF_003636"
     CDS             1539..1844
                     /gene="ulaB"
                     /locus_tag="ACHUOF_003636"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR0612654.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1854..2318
                     /gene="ulaC"
                     /locus_tag="ACHUOF_003637"
     CDS             1854..2318
                     /gene="ulaC"
                     /locus_tag="ACHUOF_003637"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR0612655.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2332..2982
                     /gene="ulaD"
                     /locus_tag="ACHUOF_003638"
     CDS             2332..2982
                     /gene="ulaD"
                     /locus_tag="ACHUOF_003638"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR0612656.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2992..3846
                     /gene="ulaE"
                     /locus_tag="ACHUOF_003639"
     CDS             2992..3846
                     /gene="ulaE"
                     /locus_tag="ACHUOF_003639"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR0612657.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            3846..4532
                     /gene="ulaF"
                     /locus_tag="ACHUOF_003640"
     CDS             3846..4532
                     /gene="ulaF"
                     /locus_tag="ACHUOF_003640"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR0612658.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4661..4936)
                     /gene="yjfY"
                     /locus_tag="ACHUOF_003641"
     CDS             complement(4661..4936)
                     /gene="yjfY"
                     /locus_tag="ACHUOF_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR0612659.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5262..5657
                     /gene="rpsF"
                     /locus_tag="ACHUOF_003642"
     CDS             5262..5657
                     /gene="rpsF"
                     /locus_tag="ACHUOF_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR0612660.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5664..5978
                     /gene="priB"
                     /locus_tag="ACHUOF_003643"
     CDS             5664..5978
                     /gene="priB"
                     /locus_tag="ACHUOF_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR0612661.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5983..6210
                     /gene="rpsR"
                     /locus_tag="ACHUOF_003644"
     CDS             5983..6210
                     /gene="rpsR"
                     /locus_tag="ACHUOF_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR0612662.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6252..6701
                     /gene="rplI"
                     /locus_tag="ACHUOF_003645"
     CDS             6252..6701
                     /gene="rplI"
                     /locus_tag="ACHUOF_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR0612663.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6772..7335)
                     /locus_tag="ACHUOF_003646"
                     /pseudo
     CDS             complement(6772..7335)
                     /locus_tag="ACHUOF_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         1757 a         1800 c         2018 g         1806 t
ORIGIN      
        1 tatttgcggg tcgcgtcaca tttaatcata aataatcttg ttgtgattac ttttgaaaat
       61 tagagtgagt gcacaacatt ccgggtgtgc ggaatacccg gttacctctt cttcaggaga
      121 tcgttatgga gatcctctac aacatcttta ccgtgttttt taaccaggtc atgaccaatg
      181 ccctgttgtt gctgggtatt gtgacctgtc tgggctacat cctactgcgc aaaagtgtca
      241 gcgttattat taaaggcacg attaaaacca taattggttt catgttgttg caggcagggt
      301 ccggcatcct caccagcacc ttcaaaccgg tggtggcgaa aatgtccgaa gtctacggca
      361 ttaacggcgc aatttccgat acttacgcgt cgatgatggc aaccatcgac cgcatgggcg
      421 atgcctatag ctgggtaggt tacgcggtgt tgttagcgct ggcgctgaac atctgttacg
      481 tgctgctgcg tcgcattacc ggtattcgta ccatcatgct gactggtcat atcatgttcc
      541 agcaggcggg gctgattgcc gttacgttgt ttattttcgg ctactccatg tggacaacca
      601 ttatctgcac ggcgattctg gtttcgctct actggggcat tacctccaac atgatgtaca
      661 agccgactca ggaagtgacg gacggctgcg gtttctccat cggtcaccag caacagtttg
      721 catcattaat tgcctataaa gttgcgccgt tcctcggcaa aaaagaggaa agcgttgaag
      781 acctcaaact gccaggctgg ctgaatattt tccacgacaa catcgtctcc acggcgattg
      841 tcatgaccat cttctttggt gccattctgc tctccttcgg tatcgacacc gtgcaggcga
      901 tggcaggcaa agtgcactgg acggtctata tcctgcaaac cggtttttcc ttcgcggtgg
      961 cgatcttcat catcactcag ggtgtgcgca tgtttgtggc ggaactctct gaagcgttta
     1021 acggtatctc ccagcgcctg atccctggtg cggttctggc gattgactgt gcggctattt
     1081 atagcttcgc tccgaacgcc gtggtctggg gctttatgtg gggcaccatc ggtcagctga
     1141 ttgcggttgg catcctggtc gcctgcggtt cctcgatcct gattatccca ggctttatcc
     1201 cgatgttctt ctccaacgcc accatcggcg tgttcgctaa ccacttcggc ggctggcgtg
     1261 cggcgctgaa gatttgtctg gtgatgggga tgatcgaaat ctttggttgc gtctgggtgg
     1321 tgaaacttac cggtatgagt gcctggatgg ggatggcgga ctggtcgatt ctggcaccgc
     1381 cgatgatgca gggcttcttc tccatcggta tcgcctttat ggccgtcatc attgtaattg
     1441 cactggctta tatgttcttc gctggccgcg cactgcgcgc agaagaagat gcagaaaaac
     1501 aattggcaga acagtctgct taataaggag ttttgattat gaccgtacgt attctggctg
     1561 tgtgtggcaa cggacaaggc agttccatga tcatgaagat gaaagtggac cagtttttaa
     1621 cccaatcaaa cattgaccac acggtaaata gctgcgcggt tggcgaatac aaaagcgagt
     1681 tgagcggcgc ggatatcatc atcgcttcta cgcacattgc gggcgaaatc accgtgaccg
     1741 gcaacaaata cgtggttggc gtgcgcaaca tgctctctcc tgccgacttt ggcccgaaac
     1801 tgctggaagt gatcaaagcg catttcccgc aggatgtgaa gtaaggacgc gccatgaaat
     1861 tacatgattc gctggcggag aataaatcca tccgcctgca ggctgaagct gagacatggc
     1921 aggacgcagt aaaaatcggt gttgacctgc tggtggcggc ggatgttgtc gagccgcgtt
     1981 actaccaggc gattctggat gcggttgaac agcacggtcc gtactttgtg ctcgcaccag
     2041 gcctggcaat gccgcacggt cgcccggaag agggcgttaa gaaaaccggt tttgcgctgg
     2101 tgacgctgaa aaagccgctg gagttcaacc acgaagataa cgacccggtg gatatcctca
     2161 tcaccatggc ggcggtcgat gccaacacgc accaggaagt gggcatcatg cagatcgtca
     2221 acctgtttga agatgaagaa aatttcgacc gtttacgtgc ctgccgtacc gagcaggaag
     2281 tactggatct cattgaccgc accaacgcgg cagcttaaga aggaattgaa gatgtcatta
     2341 ccgatgttgc aagtcgcgct ggacaaccag actatggata gcgcctacga aaccactcgc
     2401 ctgattgccg aagaagtcga cattatcgaa gtgggtacca ttctgtgcgt gggcgaaggc
     2461 gtgcgtgcgg ttcgcgatct gaaagcgctc tacccgcaca aaatcgtgct ggcagacgcc
     2521 aaaattgccg atgcgggcaa aattctttct cgtatgtgct tcgaagccaa cgctgactgg
     2581 gtgaccgtga tttgttgtgc ggatatcaac accgccaaag gtgcgctgga cgtagcaaaa
     2641 gagtttaacg gcgacgtgca gatcgaactg accggttact ggacctggga acaggcgcaa
     2701 cagtggcgcg acgcaggtat tcagcaggtg gtttatcacc gcagccgtga cgcgcaggcc
     2761 gctggcgtgg cgtggggtga agccgatatc actgcgatca aacgtctttc cgatatgggc
     2821 ttcaaagtca ccgtcaccgg tggcctggcg ctggaagatc tgccgctgtt caaaggcatt
     2881 ccaattcatg tgtttatcgc cggtcgcagc atccgtgatg ccgcttctcc ggttgaagcc
     2941 gcgcgtcagt tcaaacgttc catcgccgaa ctgtggggct aaggagcgga tatgttgtcc
     3001 aaacaaatcc cgcttggcat ctatgaaaaa gcgctccccg ccggggagtg ctggctggaa
     3061 cgcctgcgac tggcaaaaac gttgggcttc gattttgtcg agatgtcggt agatgaaact
     3121 aacgagcgcc tgtcgcgcct tgactggagc cgcgaacagc gtctggcgct ggtgaatgct
     3181 attgttgaaa ccggcgtgcg tgtgccgtcc atgtgccttt ctgcccatcg tcgtttcccg
     3241 ttgggcagcg aagatgacgc ggtgcgggcg caggggctgg agattatgcg taaagccatc
     3301 cagttcgccc aggacgttgg cattcgcgtg atccagctgg cgggctatga cgtttactat
     3361 caggaagcca ataacgaaac gcgtcgtcgt ttccgtgacg gcctgaaaga gagcgttgag
     3421 atggcgagcc gcgcgcaggt gacgctggcg atggagatca tggattatcc gttgatgaac
     3481 tccatcagca aggcgctggg ttacgcgcac tatctcaaca acccgtggtt ccagctctac
     3541 ccggatatcg gcaacctgtc ggcgtgggac aacgatgtgc agatggagtt gcaggccgga
     3601 atcgggcata tcgtcgcggt gcatgtgaaa gacaccaaac cgggcgtctt caaaaacgtg
     3661 ccatttggcg aaggtgtggt ggatttcgaa cgttgcttcg aaacgctcaa acagagtggc
     3721 tattgcgggc cgtacctgat tgagatgtgg agcgaaacgg cggaagaccc ggcggcagaa
     3781 gtggtgaaag cgtgtgattg ggtgaaagcg cgcatggcca aagcaggcat ggtggaggcg
     3841 gcataatgca aaagctaaaa cagcaggtgt ttgaagccaa catggatctg ccgcgctatg
     3901 ggctggtgac ctttacttgg ggcaacgtca gcgctatcga ccgcgaacgc gggctggtgg
     3961 tgatcaagcc cagcggcgtt gcctacgaaa ccatgaaagc agacgatatg gtggtggtcg
     4021 atatgagcgg caaggtggtg gaaggggagt atcgcccatc ttccgacact gcgacgcatc
     4081 tcgaactcta ccgtcgttac ccgtcgcttg gcggcattgt ccatacccac tccactcatg
     4141 ccaccgcatg ggcgcaggcg gggctggcga tcccggcatt aggcaccacg cacgctgact
     4201 acttctttgg tgatatcccg tgtacgcgtg gattaagcga agaagaggtg cagggtgagt
     4261 atgaactgaa cactggcaaa gtgattatcg aaacgctggg caacgccgag ccgctgcata
     4321 cgccgggaat tgtggtgtat cagcacggac cgttcgcctg ggggaaagat gcccacgacg
     4381 cggtgcataa cgcggtagtg atggaagaag tggcgaaaat ggcgtggatt gcgcgcagta
     4441 ttaatccaca actcaatcat atcgacagct acctgatgaa taaacacttc atgcgtaagc
     4501 acggtcctaa cgcttattac gggcagaagt aaaacacgcg ctgcggaaat ttccttcctc
     4561 gggagataac tggtctaatt ccgcagccgt ttttcaaaaa aagccccctg cgaagggggc
     4621 aaagcaaact atggcaatgt ttcgttggtt atacctggtg ctagcgataa atatccgcgc
     4681 tggcgtgcat attgccgtta ctccccggtt cccgcatcag aattacgtgg tagtacgttg
     4741 cgccttgcgc atctgtttct tcatttaatg cctgacgtgc ttcgctttca gtggcgaaat
     4801 tatgattgat ataaatcacg cctaagcttt gcacatcgtc catatttctg gcctggtggt
     4861 tattaatttc aatggctgcc catgtatttg cacttagcaa aagcacagcc agaagggcta
     4921 aaactcgact gaacatagat acctcctcga cggctgactt tgtgtgctct cctctgtgat
     4981 gatcttctga tttaatttta atcaatgata aagaagttga tggtgaccat ttctgatgca
     5041 gttgttcaaa aaacactgtg acggaatgtg atgaacttca aatcagcggt ttagaggtta
     5101 tttgcgaaag gggagattta tttctgctcc gcccttgagt ttagcggggt atgccagtac
     5161 tataacgcgt catttttcag ccgaccttta acacgttcct tgcctccccg ggattcggct
     5221 gacccagaca ggaggctgaa taatccgtaa ggagcaattc gatgcgtcat tacgaaatcg
     5281 tttttatggt ccatcctgac cagagcgaac aggttccggg catgatcgag cgctacactg
     5341 ctgccatcac tggtgcagaa ggcaagatcc accgtctgga agactggggc cgccgtcagc
     5401 tggcttaccc gatcaacaaa ctgcacaaag cacactacgt tctgatgaac gttgaagctc
     5461 cgcaggaagt gatcgatgag ctggaaacta ccttccgctt caacgatgcc gttatccgca
     5521 gcatggttat gcgtaccaag cacgctgtta ccgaagcatc tccgatggtt aaagcgaaag
     5581 acgagcgccg tgagcgtcgc gatgatttcg caaacgaaac cgctgatgat gctgaagctg
     5641 gggattctga agagtaattt ctgatgacca accgtctggt gttgtccggc accgtgtgca
     5701 ggactcccct tcgaaaggtc agtccatcag gaattcctca ctgccagttc gtgcttgagc
     5761 atcgttctgt gcaggaggaa gccggctttc accggcaggc atggtgtcaa atgcccgtta
     5821 ttgttagcgg acacgaaaac caggccatta ctcacagtat aacggtcggc agtcgcataa
     5881 ccgttcaggg gttcatttca tgccacaagg caaagaacgg actgagcaaa atggttttgc
     5941 atgccgagca gattgaattg atagattctg gagactagcc atatggcacg ttatttccgt
     6001 cgtcgcaagt tctgccgttt caccgcggaa ggcgttcaag agatcgacta taaagatatc
     6061 gctacgctga aaaactacat caccgaaagc ggtaagattg tcccaagccg tatcaccggt
     6121 acccgtgcaa aataccagcg tcagctggct cgcgctatca aacgcgctcg ctacctgtcc
     6181 ctgctgccgt acactgatcg ccatcagtaa tcggtcacgg tccattaata cgactttgag
     6241 aggataaggt aatgcaagtt attctgcttg ataaagtagc aaacctgggt agcctgggtg
     6301 atcaggtaaa cgttaaagcg ggctatgctc gtaacttcct ggtaccgcag ggtaaagctg
     6361 ttccagctac caagaaaaac attgaattct tcgaagcacg tcgcgctgaa ctggaagcta
     6421 aactggctga agttctggca gctgctaatg ctcgcgctga gaaaatcaat gcactggaaa
     6481 ctgttaccat cgcgtctaaa gctggcgacg aaggtaaact gttcggttcc atcggtactc
     6541 gcgacatcgc tgacgctgta actgcagctg gcgttgaagt ggctaagagc gaagttcgtc
     6601 tgccgaacgg cgttctgcgt accactggcg aacacgaagt gagcttccag gttcacagcg
     6661 aagtattcgc gaaagtgatc gtaaacgtag tagctgaata attcgttatt caacgagacg
     6721 taaaaacgcc gaccattggt cggcgttttg ctttctattt ttcgtcaggc attaatttcg
     6781 caagtagatc cattcaccat tctcttgtgt ccataagtca ggatggtttg ctggcgttag
     6841 atgcatacga tcactcctta tgtctcggtc ctgcggcact cgctcgaaac ctaaacccga
     6901 gtagattgcg gctgaatttg ataaggaacg tagaccaatt ttcaaatcat ggggtaaatc
     6961 agaatacgcg tttagcaatg cagcaatgat gaatccgagg gcattagaaa cgcctgtaac
     7021 agtgtgaagt tggtcgatat aaagatcgat tgttggtagt tcagatgagc agcaattagg
     7081 catttgggca ggaagggtta caacgtatcc tatcgtttgg ccggtatagt ctggagtact
     7141 ataaccaggt cccaaaacaa cctcgtgtat tgtgtattta ctgttgggtt cgaaggattc
     7201 gacatcttgc aataaatatt tcagttctgc ggttaacctc gcacccgcgt aagctttatt
     7261 gtatttctgg gcgtgaatat tatctaatgt agcttcatgg ttccatccaa aagaattatc
     7321 accattcatt gttactgaat cgccacgggt ttaacagaca cctcagagtc atttaagatg
     7381 g
//
LOCUS       DAZOYF010000184         7375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZOYF010000184 DAZOYF010000000
VERSION     DAZOYF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.185"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..1541
                     /locus_tag="ACHUOF_003647"
     CDS             <1..1541
                     /locus_tag="ACHUOF_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR0612664.1"
                     /translation="PTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVD
                     TYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGV
                     YSNTDAQDGIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFT
                     TFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVV
                     DRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKR
                     ILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKG
                     KLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWI
                     TSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVEN
                     NQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKAL
                     SEAK"
     gene            1593..2750
                     /locus_tag="ACHUOF_003648"
     CDS             1593..2750
                     /locus_tag="ACHUOF_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0612665.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKMMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="ACHUOF_003649"
     CDS             complement(2716..2838)
                     /locus_tag="ACHUOF_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612666.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="ACHUOF_003650"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="ACHUOF_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="ACHUOF_003651"
     CDS             4764..7124
                     /locus_tag="ACHUOF_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0612667.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7181..>7375
                     /locus_tag="ACHUOF_003652"
     CDS             7181..>7375
                     /locus_tag="ACHUOF_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612668.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
BASE COUNT         2163 a         1614 c         1568 g         2030 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaatgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgca aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7261 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7321 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattg
//
LOCUS       DAZOYF010000185         7363 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZOYF010000185 DAZOYF010000000
VERSION     DAZOYF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.186"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHUOF_003653"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHUOF_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFR0612669.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="ACHUOF_003654"
     CDS             complement(1433..1759)
                     /locus_tag="ACHUOF_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFR0612670.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="ACHUOF_003655"
     CDS             1894..2235
                     /locus_tag="ACHUOF_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFR0612671.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="ACHUOF_003656"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="ACHUOF_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="ACHUOF_003657"
     CDS             complement(2833..3543)
                     /locus_tag="ACHUOF_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFR0612672.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="ACHUOF_003658"
     CDS             3651..3956
                     /locus_tag="ACHUOF_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFR0612673.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..5568
                     /locus_tag="ACHUOF_003659"
                     /pseudo
     CDS             4155..5568
                     /locus_tag="ACHUOF_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(6298..7287)
                     /locus_tag="ACHUOF_003660"
     CDS             complement(6298..7287)
                     /locus_tag="ACHUOF_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612674.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
BASE COUNT         1945 a         1873 c         1762 g         1783 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagtgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc cctatcttct attgcactac cgcactagac
     6001 aactagtaca aaacaaaggc ccagaccatt ggtccagacc gtagttagtt aatcacagtg
     6061 acaagccacc acctgaagtc ctgctgatat tcagtccaga cttacctctg ctcatggtct
     6121 tacctccctc ggtctcctga ccttgggtat cgactacagc ttgctgagcg tcatctggag
     6181 gagctacagg aggagctaca ggaggcggga ccgaagcccc accttgagtc ccaccaccac
     6241 ctgtcaggcc cttggcgatg gacctgaagc ctttactaat gctcttaccc attggtctta
     6301 cgcttcggtc ttggtgaact tcaggcgttt gactacaggc aggacggcag tagctgagcc
     6361 agtgcccatc ttgatggtcg tagcagcttc agcgacaacg aacactttac cgaggatttc
     6421 caagtcgtgg gactgagagt cgccatcacg tgaaaggacg accgtaatat tctcaccgtc
     6481 gtgacggaag cagccgaagc tcagggccgt gaagcgttta gccagcatga atacaccctt
     6541 gtcttcaccg ttgtaatagg cgatgtctgt ccccagcata tcgacgatag cgtcttcacc
     6601 ggagaagcct tgcttagcag ccagacggga cagtgctttc agttggctct catgagcgat
     6661 aataccgtag tcagacacct cagaaccgaa agaagactct gtgttctcat acagagcgtc
     6721 catgatagcg tctgccttag cggtcaggct accagtcagg acaggaagct ctacggtctt
     6781 cagcagctta gaggattcca gaacgtcgac catgcgagcg ttgatggtac ggaccacatt
     6841 ctcacagatg ttcatagcgt cctgctgaag tgtgccctga gtgaatccgg tggatgactt
     6901 ggcgtcccag agcttggtct gctcagagat gagaatagcc tgctcgattg gttcgaccac
     6961 gatttcttgt ccagacggaa cggtcacgac ttcaggtcgc ttgccaccac ggacaggaac
     7021 gaccacagag cggtcagcca gagtgtgagc aatgattggg gtcggtaagc tgttacgggt
     7081 cggcaggact tctacaggga acgggatgtt ctcttgagcg tctttaagta caccgttcag
     7141 catagcctta accgtcttag ctaactggag ggcatagacc tgcgggtcag cgttagtcgg
     7201 cagaccttca ttgacctgaa tgttgtcggt atcggtgatg cgtactggct caatgccaaa
     7261 ctggaaagcg tctttaatcg tagtcataat aatcaatctc ctaaattagt ttaaatactg
     7321 tgagtcctca aaactgagga gtccctaaca tcgggtcagg gac
//
LOCUS       DAZOYF010000186         7234 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZOYF010000186 DAZOYF010000000
VERSION     DAZOYF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.187"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1098..2045
                     /gene="icsP"
                     /locus_tag="ACHUOF_003661"
     CDS             1098..2045
                     /gene="icsP"
                     /locus_tag="ACHUOF_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFR0612675.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(2607..2979)
                     /locus_tag="ACHUOF_003662"
                     /pseudo
     CDS             complement(2607..2979)
                     /locus_tag="ACHUOF_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            3223..3342
                     /locus_tag="ACHUOF_003663"
                     /pseudo
     CDS             3223..3342
                     /locus_tag="ACHUOF_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            3592..4458
                     /gene="ospB"
                     /locus_tag="ACHUOF_003664"
     CDS             3592..4458
                     /gene="ospB"
                     /locus_tag="ACHUOF_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFR0612676.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            4787..5527
                     /gene="phoN2"
                     /locus_tag="ACHUOF_003665"
     CDS             4787..5527
                     /gene="phoN2"
                     /locus_tag="ACHUOF_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR0612677.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            5600..5722
                     /locus_tag="ACHUOF_003666"
     CDS             5600..5722
                     /locus_tag="ACHUOF_003666"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612678.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(5880..7234)
                     /gene="ospC4"
                     /locus_tag="ACHUOF_003667"
                     /pseudo
     CDS             complement(5880..7234)
                     /gene="ospC4"
                     /locus_tag="ACHUOF_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701114.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
BASE COUNT         2290 a         1329 c         1375 g         2240 t
ORIGIN      
        1 atccaaactg ccaagcccct ttattataag taagatctgg cactggaata ttctcaaaag
       61 gactaacatc atcaccacga ccttcaacca accattttat tgcatttaaa tcccagaata
      121 gttcagtagt ggtcaatttg ggaaacgcac aatattgtac ggttacgtta tcgcaataga
      181 gtgaactctc gccctatacg gcgttttcaa ggattagtcc ttaatcggac aaccacatta
      241 aagcataata aataattaag ctttaataat atactataga tacagttcta ttataaaaaa
      301 tactatttta ggtgatgcat taaattagta gaatatttat tcttttataa aagcaccaat
      361 gaattgtcca agtaaacatg agataaaatg tgtagaggat aactgagttt ggtcacttta
      421 actgtattag tcgcagtctg actctcgact ttaaaaggat gggaattacc tgtactgata
      481 gtgagaaagc tttccattga aataagtcaa ataaaagttg atggcttcag tgctaaagtg
      541 aggtgcccgc tagccgtatt cactcgaatc ggaattcaat gtattgcttc tgccatttcc
      601 ataatatttc acgggaaaaa caaaatctga aaaactatac ttaagtgttg tattgagtca
      661 gttgagatac aaatacatat ttatcagcac acgttacgtc aaatacagga ggttgtcatg
      721 ttggtactga aaggcacgtt ggctattgca gccgtgatga caatttattg tcttatcgtt
      781 gttcttatgg aacgcttttc tgattgattt catattggcg agagaacaag aaggtgagta
      841 gaattgtcgc ggaatcctga aactatcagc ctcaggtatg aacaccaaaa ggtaggtaga
      901 gaaatctatg gcccccgtta ctttattcgc acaatgaggt atttttttat ttttacaaaa
      961 gttcaataaa tattataata aaagatatgt tcttggtaac ataaacaaat atttaattaa
     1021 aataatgctt ggcattgacg tttatcttat aaagtaagaa gatcatggac atttcaacca
     1081 aaaaagtaga gttctcgatg aaattaaaat tctttgtact tgcactttgt gtacctgcga
     1141 tctttactac acatgctacc actaactatc cacttttcat accggacaac atcagtacgg
     1201 atattagtct aggatctctg agtggcaaaa caaaagaacg cgtttatcat cccaaggaag
     1261 gggggagaaa aattagtcaa ctggactgga aatacagtaa tgcgactatt gttagaggtg
     1321 gcatcgattg gaagctaatt ccaaaagtgt ctttcggagt ttccggttgg actactttag
     1381 gtaaccagaa agcaagcatg gttgataaag actggaacaa ttccaatacc cctcaggtat
     1441 ggaccgacca gagctggcat cccaatacgc atctccgtga tgctaacgaa ttcgagctga
     1501 atcttaaagg ttggttatta aataatttgg attatcgact tggactaata gcaggttacc
     1561 aagagagtcg ttacagtttt aatgcaatgg gagggagtta tatttatagt gaaaacgggg
     1621 ggagcaggaa taaaaaaggg gcacatccta gtggtgaaag aacaataggt tacaaacagc
     1681 tcttcaaaat accttatatt ggattaactg ctaattaccg ccatgagaat tttgagtttg
     1741 gagcagaact gaaatatagt ggttgggttc tttcatctga tacagataaa cactatcaga
     1801 ctgagacaat ttttaaagat gaaataaaaa accaaaatta ctgctctgtt gctgcgaata
     1861 ttggatacta tgtcaccccc agtgcaaaat tttatataga aggctccaga aattacattt
     1921 ctaataaaaa aggtgataca tctctttatg agcaaagtac caatatatct ggcaccatta
     1981 aaaatagtgc aagtattgaa tatattggtt ttctcacttc cgcaggtata aagtatattt
     2041 tttgaattgg ttcaccgagt tgattacgtt ttcatggcta aaaattgccg cccattgtga
     2101 gtcatattcg ccctgacttt ccagaaccat acgaactgcc cgttgacgga cttcggggga
     2161 aaaacgtgta tttttagtca ttctgtttac ctctttctca gggcgtttag tctccaggat
     2221 ttccggggcg gttcaaaaaa ctcttcgttg attactgtgg acttactgtt ggcattaccg
     2281 accctgagac cggagaaata agaaccgctt agatcatcgt ggctgttctc ggggtatcaa
     2341 gttacacatg ggcagaggct acccaaggtc tactattatc gcctgaaagc gctgatggaa
     2401 cagtgctatc agggacatgc tgatgaaaat acctaaataa tcagccagca ctctatcttt
     2461 ccaaatccac agcatagcaa agagagcaaa agagcctgta aattcagaaa ttaaggaaac
     2521 gcctcttggt gtacataatc tatcttatgt taaatgaaat ctaatttctt gattatatgc
     2581 tggatcgttc gtgggtggct ttgcccctat atttccagac acctgttatc attggcccgc
     2641 tgccgcagat attcccgtgg cgagcgataa tccagcgcac tatgcggatg ccattcgtta
     2701 taatgctcga atgcctctgc aaggttcttt gctgccgtta acccgtcagg tttgggcatg
     2761 atactgatgt agtcacactt tattgttttc acgaagctct ttgctattct gttactctcc
     2821 ggactccgca ccgccgtgtt cttcggttca acaaagtccc aacatccggg tgaactggcg
     2881 tgtttcatta gctcggtagc atgaaccatt atccgtcagt cacttcactg gagacgccgg
     2941 aagctcgttg ccgaagcggt gttccaccgc tcccagcatg tcgccaacgg gagagcatct
     3001 acggtctgat tgaactgttt attcaaattg tccaccgtct gtcagtacgc tctgaacgtc
     3061 ggctggttaa aacactgctc gctgattttc agagggttca cggtaaaaca gcacttttat
     3121 tccggattgc ggaagcggct ctgaataatc cggatgggct ggttaaagag gttgtgtacc
     3181 atctgcatat tgttgaacca gatcgcagcg gtaagagaag ttcatcatac ttggcgcagt
     3241 tacgcgatgt ttctgctaga ggtgatgccg taaagaacgg cagaacatta ccggaacaag
     3301 atagcggtct acctgcactg gtatcagacc cggggcttcc ccggatgatc tccacggtac
     3361 tttaggtact gtgtctatat tatgtaggta ggatcaatta actttgtagt tgaaattctt
     3421 tcattgaaat aatgaataaa aaagacaaac cgcattaaac aatgccagaa tataattgca
     3481 tttttgttcc gtttttttaa atcatagtat acaaagaagc ttgcatacta tatgaaaata
     3541 taacaccctt tactatgatg tttataaaac aatatatgga gtcatgtagg tatgaattta
     3601 gatggtgtta gaccatactg tagaatagtc aataaaaaga atgaaagcat atcagatatt
     3661 gcatttgcac atataataaa aagggtaaaa aattcatcat gtactcaccc aaaagcagca
     3721 ttggtttttt taggagagaa aggtttttgt gatagcaatg atgttctatc tattatggga
     3781 caacaaatac caagagtatt taagaacaag atgttatatg attatgtttt taaaaatgaa
     3841 aaaagtaaaa atgattttct aaaaatggct gaatcatggc taccacagag tgaaccaata
     3901 gtaataaata atgatgatga cgcattgaat gctgctgctt atttttctgt aaaaaaagcg
     3961 aaaataaaaa cagtaaacga tactgatttt aaagagtata ataaggttta tattcttggg
     4021 cacggtagtc ctggttctca tcaattaggc cttggttcgg aacttattga tgtacaaaca
     4081 atcatttcaa gaatgaaaga ctgtggtatt ctaaatgtga aagatatccg ttttacttca
     4141 tgcggctccg ctgataaagt ggctcctaaa aattttaaca atgcccctgc tgaaagtctt
     4201 tcttgtatcc ttaactctct gccttttttt aaggaaaaag aatctttgct agagcagata
     4261 aaaaaacacc ttgaaaacga tgagtcattg agtgatggtc taaaaatatc cggctatcat
     4321 ggatatggag ttcactatgg tcaagagctt tttccctact cacattatcg ttcaacttca
     4381 attcctgctg atccggagca tacagtaaaa agaagctctc agaaaaagac ttttattatt
     4441 aataaagaac tggattagta taaaattttt aacctatagt taattgtttg catttaagat
     4501 gaagttattt tatctttagt agaaaagtgc ttccctatga gggaaaatca caaatcatca
     4561 taatacagag acaaaacgat acgaaaaaat agataaaaaa catcattctt ttaccacatt
     4621 attttccgtg aatatgaaaa ataatgttat tactttaata taagactatt ttttgttttt
     4681 ccatcactct gttcaaattt ttccgcatga cttgtgtttt ttgtaataca gctcgttttt
     4741 tacatctgac caaaatcatc aattaattat gctaaggaaa taaattatga aaaccaaaaa
     4801 ctttcttctt ttttgtattg ctacaaatat gatttttatc ccctcagcaa atgctctgaa
     4861 ggcagaaggt tttctcactc aacaaacttc accagacagt ttgtcaatac ttccgccgcc
     4921 tccggcagag aattcagtag tatttcaggc tgacaaagct cattatgaat tcggccgctc
     4981 gctccgggat gctaatcgtg tacgtctcgc tagcgaagat gcatactacg agaattttgg
     5041 tcttgcattt tcagatgctt atggcatgga tatttcaagg gaaaataccc caatcttata
     5101 tcagttgtta acacaagtac tacaggatag ccatgattac gccgtgcgta acgccaaaga
     5161 atattataaa agagttcgtc cattcgttat ttataaagac gcaacctgta cacctgataa
     5221 agatgagaaa atggctatca ctggctctta tccctctggt catgcatcct ttggttgggc
     5281 agtagcactg atacttgcgg agattaatcc tcaacgtaaa gcggaaatac ttcgacgtgg
     5341 atatgagttt ggagaaagtc gggtcatctg cggtgcgcat tggcaaagcg atgtagaggc
     5401 tgggcgttta atgggagcat cggttgttgc agtacttcat aatacacctg aatttaccaa
     5461 aagccttagc gaagccaaaa aagagtttga agaattaaat actcctacca atgaactgac
     5521 cccataaagc tggacagcct gtatcaggct atggagggcc catagacaaa tctaccctat
     5581 atgagcatag gaggagtcta tgggcacacc acgttttacc cctgaattta agggattact
     5641 ggaaaggctg ggacatatcc ctccggcaga agcagaaaaa gcttattatg ctgccatcgg
     5701 aaacgatgat ctggcaacct gagttcacag ataaaacatt ctctaggaaa ctcggggcgg
     5761 ttccgttcac cacatgcaat gtggtgttgc aggggaacgg tctgcaccat cccctatgtc
     5821 gatcaatata acagaaatga caacttcaga ttcagggcac aacctaaata tattttaggt
     5881 cacctctcaa atcgtttgcc tgataccgct ccatttttta ataaaaaaat caatcatttt
     5941 tgaatctttg cttttcattg cattgtcaag catagtgtcc ccactatttg ctttttgaaa
     6001 ttttttgttt acatcaacca atccattatt gataaaatat tctagcactt tatagttggc
     6061 accatcttta ctaagtaagt attcaacatc atacagatta caatatctat tgtttttcat
     6121 tttctccatc tctgcgacat cttgttttgt aaaattaaat ttagatagta aataaagtgc
     6181 tatatcagct tttttattgc ctaaagaata agcaagggcc tgatgagcca tttttttatc
     6241 agtcacttta ttgttaatct cttcataatt agatgcttta atcgcatcct ccaaagatat
     6301 gtctctgagt tttatttttt tgaagttatc tgtacttagc attcttggta tatgatactc
     6361 aagctgaaac acaaagttta taattctatc cagactaaca ggatcaatat ttttgttata
     6421 acaaaactct ctgaatcctt gatctttact ctttcttata aaatcaataa gatgcaaccc
     6481 aattccaagc ctcatatctt tagtgttaaa tattggaaca tcagttttcc cctgattacc
     6541 atgcacatac ctgctaactt catccacaac ctctttaaag ttatctaaga aaaatgattg
     6601 gtgctcatgg taaaaaacag gtggaatagc attatcaaag tgatcggtta atgtcatata
     6661 tccatatgga gagtcatgat caatgatata cgcattggcg ccaaaatcta cagtatgatg
     6721 tgttttattt agtgggagtt tttcttgatg attggaaatt tcgaccccaa aaaaaacaaa
     6781 atcgtggtta gagatttctt taatatcctc agagtatgta ttcttatgtg tttgtatatt
     6841 ttcagactga attttatcaa aggatttaat attaagaata ttatttgaca gattagagtt
     6901 actttgatgt ctgaagttaa aattagaatt aataataata gaaagtaagt gacgttcgtc
     6961 tgaagaaaga ggatgaactt tttgtattat ctcagagata tagtgcctgg acaccttatc
     7021 gagatgctca agtgaccgaa atgatgaccg tgctccagat ggcgcttcaa gagaaagatt
     7081 tagtgatttg aaactggtcc cttgagaaaa cattttgcta taactttgcg ctgctataga
     7141 cttccttaaa aagtttttca cagtattagc acagtgatct aaatgttttt cacttatgcc
     7201 agaagaagat gagtttgtta cgcgtaatac attt
//
LOCUS       DAZOYF010000187         7228 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZOYF010000187 DAZOYF010000000
VERSION     DAZOYF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.188"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..190)
                     /locus_tag="ACHUOF_003668"
     CDS             complement(<1..190)
                     /locus_tag="ACHUOF_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR0612679.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="ACHUOF_003669"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="ACHUOF_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR0612680.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="ACHUOF_003670"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="ACHUOF_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR0612681.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="ACHUOF_003671"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="ACHUOF_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR0612682.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="ACHUOF_003672"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="ACHUOF_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="ACHUOF_003673"
     CDS             complement(3262..3381)
                     /locus_tag="ACHUOF_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR0612683.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACHUOF_003674"
     CDS             complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACHUOF_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR0612684.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /gene="ymgI"
                     /locus_tag="ACHUOF_003675"
     CDS             3628..3801
                     /gene="ymgI"
                     /locus_tag="ACHUOF_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR0612685.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="ACHUOF_003676"
     CDS             3803..4147
                     /locus_tag="ACHUOF_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR0612686.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="ACHUOF_003677"
     CDS             4151..4486
                     /locus_tag="ACHUOF_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR0612687.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="ACHUOF_003678"
     CDS             complement(4543..6864)
                     /locus_tag="ACHUOF_003678"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0612688.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="ACHUOF_003679"
     CDS             complement(6981..7190)
                     /locus_tag="ACHUOF_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612689.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         1960 a         1718 c         1610 g         1940 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatagcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcct
//
LOCUS       DAZOYF010000188         7188 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZOYF010000188 DAZOYF010000000
VERSION     DAZOYF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.189"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="ACHUOF_003680"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="ACHUOF_003680"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR0612690.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(987..2345)
                     /gene="hprS"
                     /locus_tag="ACHUOF_003681"
     CDS             complement(987..2345)
                     /gene="hprS"
                     /locus_tag="ACHUOF_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR0612691.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="ACHUOF_003682"
     CDS             complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="ACHUOF_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR0612692.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3149..3562
                     /gene="hiuH"
                     /locus_tag="ACHUOF_003683"
     CDS             3149..3562
                     /gene="hiuH"
                     /locus_tag="ACHUOF_003683"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR0612693.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3671..4675
                     /gene="msrP"
                     /locus_tag="ACHUOF_003684"
                     /pseudo
     CDS             3671..4675
                     /gene="msrP"
                     /locus_tag="ACHUOF_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4676..5311
                     /gene="msrQ"
                     /locus_tag="ACHUOF_003685"
     CDS             4676..5311
                     /gene="msrQ"
                     /locus_tag="ACHUOF_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR0612694.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="ACHUOF_003686"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="ACHUOF_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR0612695.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2032 a         1629 c         1507 g         2020 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt
      961 aacttatggc gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg
     1021 ttatgcttac tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg
     1081 actaaagaaa ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat
     1141 ctacggaaga gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta
     1201 agagagccgt tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat
     1261 cgaatggcgt taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag
     1321 atttgttgat tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga
     1381 tagtccaaaa gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca
     1441 ttgtttttgt ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc
     1501 agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg
     1561 agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga
     1621 tcgtcagcaa attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc
     1681 aacgcctgcc caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg
     1741 ctatcgttat aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc
     1801 gtcctgatta acaacggact taatactgag cacagtataa tggcgacaat gcaaattata
     1861 atgctattaa ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca
     1921 gtaaccgtta atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta
     1981 tcattaataa tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta
     2041 tttaacatgt catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga
     2101 atgatcaaaa tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct
     2161 ggatttaccc catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta
     2221 tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccatac aattccagcg
     2281 ccagcaacag actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt
     2341 ttcattattt ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct
     2401 tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat
     2461 tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa
     2521 tctcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa
     2581 tactgatatt gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt
     2641 ctaatgttga attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact
     2701 cagaaaatga aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc
     2761 tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta
     2821 acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg
     2881 cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga
     2941 cataacccgc ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa
     3001 taagtagaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt
     3061 tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa
     3121 aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc
     3181 aacggcagcc ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt
     3241 gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa
     3301 gaaagcggat aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat
     3361 taaggcactg tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac
     3421 cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt
     3481 tcatattaat aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta
     3541 ttcaacctat cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag
     3601 ggaggtgact ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt
     3661 gtgatggctt atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt
     3721 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc
     3781 tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc
     3841 cgcccccgcc ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc
     3901 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     3961 agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     4021 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct
     4081 ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat
     4141 tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     4201 cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg
     4261 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     4321 cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt
     4381 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     4441 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     4501 ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt
     4561 tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta
     4621 cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg
     4681 tctgacagca aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc
     4741 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga
     4801 tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac
     4861 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     4921 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt
     4981 gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat
     5041 tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa
     5101 actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc
     5161 aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct
     5221 ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6481 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6661 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6721 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
     6781 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
     6841 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag
     6901 ggggatttga acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga
     6961 gtcgaaaaat caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga
     7021 aaaatcaatg aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac
     7081 ctgttttcag gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct
     7141 cagcttattc cgttttgcct gtgtgttgaa taattccact gcagttat
//
LOCUS       DAZOYF010000189         7183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZOYF010000189 DAZOYF010000000
VERSION     DAZOYF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.190"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(371..1198)
                     /locus_tag="ACHUOF_003687"
                     /pseudo
     CDS             complement(371..1198)
                     /locus_tag="ACHUOF_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHUOF_003688"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHUOF_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFR0612696.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHUOF_003689"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHUOF_003689"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFR0612697.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHUOF_003690"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHUOF_003690"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFR0612698.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHUOF_003691"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHUOF_003691"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFR0612699.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHUOF_003692"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHUOF_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="ACHUOF_003693"
     CDS             7090..>7183
                     /locus_tag="ACHUOF_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HFR0612700.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAZOYF010000190         7115 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZOYF010000190 DAZOYF010000000
VERSION     DAZOYF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.191"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..127)
                     /locus_tag="ACHUOF_003694"
     CDS             complement(<1..127)
                     /locus_tag="ACHUOF_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612701.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQ"
     gene            205..1299
                     /gene="ddlA"
                     /locus_tag="ACHUOF_003695"
     CDS             205..1299
                     /gene="ddlA"
                     /locus_tag="ACHUOF_003695"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0612702.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1323..1535)
                     /locus_tag="ACHUOF_003696"
     CDS             complement(1323..1535)
                     /locus_tag="ACHUOF_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR0612703.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1795..2103
                     /locus_tag="ACHUOF_003697"
     CDS             1795..2103
                     /locus_tag="ACHUOF_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR0612704.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2162..3256)
                     /gene="yaiW"
                     /locus_tag="ACHUOF_003698"
                     /pseudo
     CDS             complement(2162..3256)
                     /gene="yaiW"
                     /locus_tag="ACHUOF_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
     gene            complement(3269..4489)
                     /gene="sbmA"
                     /locus_tag="ACHUOF_003699"
     CDS             complement(3269..4489)
                     /gene="sbmA"
                     /locus_tag="ACHUOF_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR0612705.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4841..5998
                     /gene="ampH"
                     /locus_tag="ACHUOF_003700"
     CDS             4841..5998
                     /gene="ampH"
                     /locus_tag="ACHUOF_003700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR0612706.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5999..6502)
                     /locus_tag="ACHUOF_003701"
     CDS             complement(5999..6502)
                     /locus_tag="ACHUOF_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0612707.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(6590..>7115)
                     /locus_tag="ACHUOF_003702"
     CDS             complement(6590..>7115)
                     /locus_tag="ACHUOF_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0612708.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         1799 a         1819 c         1723 g         1774 t
ORIGIN      
        1 attgccattt aagccaggaa taaattagct tcccgttcat catttttgtg atcttgcgca
       61 catctttgag ataacgcact ttcttcacca gtacgttgca cgctttgtca gcagaagatt
      121 gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc gtggtaatgt atgcactttg
      181 tgttagacgg gataggtttt taagatggaa aaactgcggg taggaatcgt ttttggtggt
      241 aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa acattgtcga tgccattgat
      301 aaaagtcgct tcgacgttgt gctgctgggc attgataaac aagggcaatg gcacgtcagc
      361 gatgccagca attatctgct aaatgcagac gatcctgccc atattgcgtt gcgcccttcg
      421 gcgaccagcc ttgcgcaggt gccaggtaaa catgagcatc agcttatcga cgcgcaaaac
      481 ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg tccacggtac gctgggcgaa
      541 gatggttcct tgcagggaat gctgcgggtc gccaatttac cgtttgtagg ttctgatgtt
      601 ctggcttcag cagcctgtat ggataaagat gtcaccaaac gtctgctacg cgatgccggg
      661 ctgaacattg cgccatttat taccctgacg cgcgctaatc gtcacaacat cagttttgcc
      721 gaagtggagt ctaaactggg gttaccactg tttgtaaaac cggctaatca gggctcttct
      781 gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa ttgccgtcga tctggcgttc
      841 gagttcgatc ataaagtgat cgttgagcaa gggatcaaag gtcgtgagat cgaatgcgca
      901 gttctgggca acgacaatcc gcaagccagc acctgtggcg agatcgtact caccagcgat
      961 ttctatgcct acgacaccaa gtacattgac gaagatggcg cgaaagtggt agttccggca
     1021 gccattgcac cagagatcaa cgataagatc cgggcgattg ccgttcaggc ttatcagacg
     1081 ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa ccccagagaa cgaagtggtg
     1141 atcaacgaga tcaacacact gcctggcttc accaacatca gtatgtatcc gaagctgtgg
     1201 caagccagcg gtctgggtta caccgatctg atcacacgtt tgattgagct ggcgctggag
     1261 cgtcacgctg cggataacgc attgaaaacc acaatgtaat attttaacgc ccgataatat
     1321 tcttattggg cgttttcagt ttcttcatcg cgacgacgaa taatcaaccc cgccagccag
     1381 aaactaatca cccacgtcac cagaccgacg gcataggtct gccaaccttt tgcttcaaat
     1441 cccagtaccc ccaccacgcc attaagaatg aatataaggc caatggcgaa cgcatagtag
     1501 tgccagtcac ggcggatttt tacaggcaga ttcatggcag ctccgttaac gaaaaacaac
     1561 atccttgcat attctgtaac aaccgtctgt ggtgtagaca acattttcgt aattattaat
     1621 aaatttaact taaggttgcc attttgtgac tcacaacgaa gattcacgct tcgggaatat
     1681 tcccaacatc aatttaccat gattctgata ttgacaatct aaatgacctg tcagacttgc
     1741 cataagccgc tggtatcaca ccagaagaga agcagatgac ttccggaggt ttttatggct
     1801 gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc gcaatgcctc ttcaacgcct
     1861 ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt gggctggggc gcaattgctg
     1921 aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa tgcaggtcca ggaaacaggt
     1981 cgcccacggg tggaaattgg tttaggtgtc ggcaccattt tcgggctgat cccgttttta
     2041 gcaggctgcc tcatttttgc agtggtggcg ctatggctgc actggcgaca tcgccgccag
     2101 taaaatttac gttgccagat gcggcctgaa acgccttatc tggcctgaca ttcctgtttg
     2161 atcactgttt catacaacgc gcccgccgtt cgtctacgcg cttcgcaaac caggccgtag
     2221 tcaggttgcg ggtgattttc gggctttcca gttgaatgcc aggcaacatt tcgcggggta
     2281 gtgatttacc cgttttcgct tcggcaagtt gataaacttt cttgtacagc gccgtctcct
     2341 caaaagagaa gctgtcacct ttctctaact gacggcgaat ttcgctgtcg ctcatcccca
     2401 gttttcccgc cagtttgcgc gttgccagtt cggttttccc gggttcttta ctgtcgtaac
     2461 gaattaaatc accgtccagt gccagcttca cgccgctggc cttactgact gcgttctgaa
     2521 acgcggcatt acggctggcg taccagccag cgttaaaatc agcaaagcgg tatatcggtg
     2581 cgctatagct ggcgggataa ttcagtaaat ggtaagtacc aaaccacaac ccgccgcggc
     2641 gactgaagac ttcctgacga actgtaccgt ccattttcca cggataccct ttggtatgct
     2701 gttcggcaaa agcaatgctg acctgcatcg gcccaccggt gcgcaccgga ttaagcgaac
     2761 caaacagcgt ctgccccatt ggtaccatgc tgatcagatc gtcaaaaatc gcgctcaatt
     2821 gcttttctgt acgcaccgaa tctaaccgtt cgctataact tttgccgttt ggcgatttga
     2881 tttttagcgc ggtatgaacc agaaaagcag gaatgtgcat ccgttcagca cgacggtcaa
     2941 tctcttgcta ggcgatcttg cttaagcccg gaacggccgg atctgcctga taatttgact
     3001 cctgctgcgc caccgccagc accgagtaga cgttttccag cgtcggtgcc agcccctgac
     3061 tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg gtctttcacg ctcgcaggca
     3121 tcttctgccg cacgacgctc gctacatcta tcgctttttc gccctttttc aacggttgcg
     3181 gagcctgact actacacccc gctaaaacca gaaccgccaa caatgacagc gaagatagat
     3241 gattgacgcg cgacattact tctccttttt agctcaaggt atgggttact tcctgaattt
     3301 tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt gtagatagac atcaactcaa
     3361 ccagtgtggt ccatgagtta atcaggtact ggaaagcacc gcgaacctga ccaaaaacgt
     3421 tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc aacaattgac ggaaacagca
     3481 agaacaaacc gaaaacgtta tcgacctgca aatagagaat gcgggcgatg ttgaaataca
     3541 tatagtgaaa atagaggcga aaatagtttt tccgtacggc gctaaacagc tcgcgtaccg
     3601 taggcggcgt cgcgcgcgtg gcatcgtctt caccataaac cagctcttta cggtaggcgg
     3661 cctctactcg ctggttttta aactccagcc ccggcagttt gatccctacc actgccagca
     3721 atccggtccc catcagcgac cagacgatag cggcaatcac cagaccatac ggaatgtgcc
     3781 cgataatcgg cagctccggc acatgcgcgg agagcgttac cagcaccggc aggaaggcga
     3841 tcaacgtcat gatggcgttg ataaaactga cgcccatatt ctccagcgtt gaagcaaaac
     3901 gcatggtgtc ttcctgcaca cgctgtgcgg caccttcgat atgacgcagt tgttgccagt
     3961 tcgccatgta atattcgttc atcgccgtac gccagcggaa cacgtagtga ctgacaaaga
     4021 agttgttcag tacactgatc accaccgcga tcagcgcaat ccccagaaag acgcccactt
     4081 cgcggtaaaa ttgttcgata gtgactttat gcggcgaact tagcgcggtt tgaatcagat
     4141 catagaacgg cgcataccag gcgttgacgg cgaccccgac ttccaccaaa aaccaagtga
     4201 cgaagataat cagcgcagta ccgagtattg accagtattg ccaacggtgc gggctgtaga
     4261 taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta gtaagcgtaa aaaatcagga
     4321 aatccaacga ccagaaacgc gcggcgctaa tcgggatctg cccggaagcg ccggtgatac
     4381 gtgccaccca gtcaccccca ccggcttgcc agaagataac ggcaatcaat gcccaaacaa
     4441 aggccgagag aaaaaacgtt cccggctttg ggaaaaaaga cttaaacata tgcactcctg
     4501 ctaacttctt atcgttttaa caactgctgt gtattaaccg catgaccgct acaaagcgtc
     4561 agccaaatga aaccgcccat tgtgacccat agcaaggcgc ttagttcgcc agcctgacca
     4621 taagaattgt ttcgacggat ttcacaaacc aaagattgtt tacataagcg gattaacacg
     4681 cgcaggatca caaatgcagg cattggtgag caggaaaaag atcgtgttta cgcgccgtta
     4741 cgtttttaac actattattg cactaattcg ccctttgcaa tccatcaatg agtagtataa
     4801 atacgctcag ttaccttcat tcaatctatg gacaccaccg ttgaaacgta gtctgctttt
     4861 ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat gcggcgcagc cgatcactga
     4921 accggagttt gcctctgata ttgtcgatcg ttatgccgat catatttttt acggcagcgg
     4981 tgccacgggg atggcgctgg ttgttatcga cggtaaccag cgcgtctttc gtagttatgg
     5041 tgaaacgcga cctggtaata acgttcgccc gcagctggat tcggtcgtgc gtattgcttc
     5101 cctcaccaaa ctaatgacca gtgaaatgct ggtgaaattg ctcgaccagg ggaccgtaaa
     5161 gttaaacgat ccgctaagta aatatgctcc gccaggcgca cgcgtgccga cctacaatgg
     5221 gacgcccatt accctagtga atctggcaac ccataccagc gccctgcccc gtgaacagcc
     5281 cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg cgcgagcaac gctggaaata
     5341 cctttctacg gcgaagctga aagccgcgcc aggcagtcag gcggcgtact ctaaccttgc
     5401 gttcgacctg ctggcagatg cactggcgaa tgcgtccggc aagccttata cccagttgtt
     5461 tgaagagcag attacccatc cattggggat gaaagacacc acctacaccc cctcaccgga
     5521 tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt ccgtgcaata acacgctggc
     5581 ggcaattggc agcggcggcg tctattccac gcctggcgat atgatgcgct ggatgcagca
     5641 gtatttgtca tctgattttt atcagcgcag taaccaggca gatcgcatgc agacgttgat
     5701 ttatcagcgc gcgcagttta cgaaagtgat tggcatggat gtccccggca aagcggatgc
     5761 gcttggttta ggctgggtat atatggcccc gaaagagggt cgtccgagga ttattcagaa
     5821 gacaggcggt ggtggcggtt tcattaccta tatggcgatg atcccgcaga agaatattgg
     5881 cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt aaaaatatga gcgatggcat
     5941 caatgatctg gtgaccgagc taagcgggaa taaaccgttg gttatccccg catcctgatt
     6001 aatactctga aggcaaacgg ttgatagcga ccagtttgcc tttattcatt tcgatatagc
     6061 cgcctttacg caaagcagcg agaacttctg cgacgacaga acgcgatatg cgtgtacgtt
     6121 gatggatata attcatcaca ccaatacgtg atcgcaattc ttcattccag tcaatcattg
     6181 ataataatgt tgcgcgggtt tgtttggctg gtagatgata tcccgtacaa tttccttctg
     6241 atattaattt gtatggtata tcgtttttca ttaaaccatc agccagcccc ataatataag
     6301 gagcctgggt aataccgata agtacgtttt cttctctacg cagagagata actccttcta
     6361 aaataacgaa agtatcttcg ttattgacat cactggaaaa tataacttgc ttttcattat
     6421 taaactcgaa gcgcgtaccg tatctggaca aacatttatc gagcttacca aattcctgaa
     6481 gaggtttaac tacagataac atttgcgcgt cctttgcagt aatgcccgtc aaatccttga
     6541 cgggcattat ttagattaaa ttaccagtat ttcttcggag tgaagaatat taccaagtat
     6601 atttaacacc cacgttcgcg gaccagtctt gatctacgtc accaccaccg aggtagttag
     6661 catcggtata ggcgctgaag ttcttggtga agctgaactg agtacccaga ccaacacgta
     6721 ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt cacatcgtta tcgttgttag
     6781 agtcgtcgta gacgtaagcc agtttgaagt acggagtcag agcctgatct tcgctgtagg
     6841 tgaaggtata acctgcatct acacccagtt cataacgcat gctgtcgtaa ggctgaccgt
     6901 caactttcat gtcgttgctc agctggtagt catcaccaga ctggaacaga ccagaaatgc
     6961 tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa gtcgtaaccg gttttcaaac
     7021 cgaagcccca agcgtcggag ttggtgctac cgtcaacgta agtaccgttg ctcatggttg
     7081 cagacaggtc gttgttgaag tgagagtagc tcaag
//
LOCUS       DAZOYF010000191         6890 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZOYF010000191 DAZOYF010000000
VERSION     DAZOYF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.193"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(12..1310)
                     /gene="exuT"
                     /locus_tag="ACHUOF_003703"
     CDS             complement(12..1310)
                     /gene="exuT"
                     /locus_tag="ACHUOF_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFR0612709.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            1795..3207
                     /gene="uxaC"
                     /locus_tag="ACHUOF_003704"
     CDS             1795..3207
                     /gene="uxaC"
                     /locus_tag="ACHUOF_003704"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR0612710.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            3222..4709
                     /gene="uxaA"
                     /locus_tag="ACHUOF_003705"
     CDS             3222..4709
                     /gene="uxaA"
                     /locus_tag="ACHUOF_003705"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR0612711.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            4792..5343
                     /locus_tag="ACHUOF_003706"
     CDS             4792..5343
                     /locus_tag="ACHUOF_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR0612712.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(5348..6592)
                     /gene="sstT"
                     /locus_tag="ACHUOF_003707"
     CDS             complement(5348..6592)
                     /gene="sstT"
                     /locus_tag="ACHUOF_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR0612713.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         1763 a         1800 c         1820 g         1507 t
ORIGIN      
        1 tacgttaaac gttaatgttg cggtgcggga tcgttatgtg tttcctgcgc cacctcaatc
       61 gccggtttgt tctgcaatac ggtccagata accagcgcac ccagcaggtc aaatactgcc
      121 agaactgcga acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca
      181 aacagcgtac ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg
      241 ttacgaccga acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgggca
      301 aaaccaccga tacacagcag cataattgcg acatacgggt tggtgaacag accgatcata
      361 cccgggccaa tcatcagcac agcacccagc gttacgacca tcttacggga aacgatcagg
      421 ttcacaccaa accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg
      481 tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa
      541 actttaaaca tgaacagcgg gatccacgcg ttaaaagtac cccaggccgg ttctgcaagg
      601 aaacgcggca gtgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt
      661 ttcttcgccg tgctcacctg gtgctgggct tcctgaccat taataatata gtcgcgttct
      721 tcatcggtca gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc
      781 cagataaagc tcaatgcacc ggagatgata aatgccatct gccagctgtg catcacgatt
      841 gcccatacca ccagcggcgg cgcaatcatc gcaccaatcg aagaacctac gttaaagtag
      901 cctactgcga tggaacgctc tttcgccggg aaccattcgg agctggcttt cagacccgcc
      961 gggatcatcg ctgcttccgc ggcaccgacc gcaccacgag caacagctag gccaccccag
     1021 ctacctgcca gcgcggttgc accacagaac acggcccaca gcacagcaaa cattgcataa
     1081 ccgattttcg tacccagcac atccagtaca taacctgcta ccggttgcat gaccgtataa
     1141 gcagcagaat aggctgcgat gatataggaa tactgttggg tggagatgtt taactcttcc
     1201 atcagagttg gcgcagctgc cgccacagtg ttacgcgtca ggtaaccaag cacggtgccg
     1261 agcgtcacca gtgcgatcat ataccaacgt aaccctttaa ttttacgcat gtaaaacctc
     1321 atcgttttgt tatatccgca ggaagcgcgg ccatcatccc gccagaggct gaagatgtta
     1381 gttacgaccg ggtaaccgaa aaaaaaaacc ggcacgcccc gaacgttgcc atgaaagttt
     1441 cgtgctaatt cggcttccgt accggttaac ccgattcatc taacaaatga atgaatcggc
     1501 aatttgcatt ccgtcgactt atgatttgcg acggcagaaa gataacttgt catacaacct
     1561 taaaaggtga gagccatcac aaatgtgcga atatttgtag ggacattacc tgacgacagc
     1621 gaggccagta ctggcgcggc ctgcagcgag atttaccact ttgagagtaa tttttttaac
     1681 tacgtttatt gatctaactc acgaaaatat cttcggactc tggaaattgg tgtgataact
     1741 ttgtcagcat cgcaccataa gcaagctagc tcactcgttg agaggaagac gaaaatgact
     1801 ccgtttatga ctgaagattt cctgttagat accgaatttg cccgccgtct gtatcacgac
     1861 tacgcaaaag accagccgat tttcgattac cattgccatt tgccgccgca gcagattgcg
     1921 gaagactatc gttttaaaaa cctgtatgac atctggttga agggcgatca ctacaaatgg
     1981 cgcgctatgc gtaccaacgg tgtggctgag cgtctgtgta ctggtgatgc gtctgaccgt
     2041 gaaaaatttg acgcctgggc ggcgactgtt ccgcacacta tcggcaaccc gttataccac
     2101 tggacgcacc tcgaactgcg tcgtccgttc ggtatcactg gcaaattgct ttctccgtca
     2161 actgccgatg aaatctggaa cgaatgtaac gaactgctgg cgcaggataa cttctccgcg
     2221 cgcggcatca tgcagcagat gaacgtgaaa atggtcggca ccaccgatga cccgatcgat
     2281 tctctggagc atcacgcaga gatcgccaaa gacggctctt tcaccatcaa agtgctgccg
     2341 agctggcgtc cggacaaagc cttcaatatc gaacaggcga cctttaacga ctacatggcg
     2401 aagctgggcg aagtttccga taccgacatt cgccgctttg ctgacctgca aactgccttg
     2461 accaaacgtc tggatcactt cgccgctcac ggctgtaaag tgtctgacca tgcgctggac
     2521 gtggtgatgt ttgctgaagc gaacgaagcg gaactggaca gcattctggc gcgccgtctg
     2581 gctggcgaaa ccctgagcga gcacgaagtg gcacagttca aaactgcggt actggtgttc
     2641 cttggtgccg aatatgcacg tcgcggctgg gtacagcagt accacattgg cgcgctgcgt
     2701 aataacaacc tgcgtcagtt caaactgctg gggccggatg taggctttga ctccatcaac
     2761 gaccgtccga tggcggaaga gttgtctaag ctgctgagca agcagaacga agaaaacctg
     2821 ctgccgaaaa ccattctcta ctgcctgaac ccgcgcgata acgaagtgct gggcaccatg
     2881 atcggtaact tccagggcga aggtatgccg ggcaaaatgc agttcggttc cggctggtgg
     2941 tttaacgatc agaaagacgg tatggaacgt cagatgaccc aactggcgca gctcggtctg
     3001 ctgagccgct ttgtcggtat gctgactgac agccgtagct tcctgtcata cacccgtcac
     3061 gaatacttcc gccgcattct gtgccagatg atcggtcgct gggtggaagc gggcgaagca
     3121 ccggcggaca tcaacctgct gggcgagatg gtgaaaaata tttgctttaa caatgcgcgt
     3181 gactacttcg ccattgaact gaactaaggt ctggggttga tatgcaatac atcaagatcc
     3241 atgcgctgga taacgtcgcg gtcgctttag cagatttggc tgaaggcaca gaagtcagtg
     3301 tcgataacca gactgttacg ctgcgccagg atgttgctcg tggacataaa tttgcgttaa
     3361 cggatatcgc aaaaggggcc aacgtcatca aatatggcct gccgattggt tatgcattgg
     3421 cggatattgc ggcgggggaa cacgtccacg cccacaatac gcgcacgaat ctgagcgatc
     3481 tggatcagta tcgctatcaa cttgattttc aggatctgcc tgcgcaagcg gcagatcctg
     3541 aagtgcagat ctatcgtcgc gctaacggcg atgtcggggt gcgtaacgag ctgtggatcc
     3601 tgccaaccgt gggctgtgtc aacggcatcg cgcggcaaat ccagaaccgt ttcctgaaag
     3661 agaccaacaa cgccgaaggt accgacggcg tgttcctctt cagccacacc tatggctgct
     3721 cacaactggg cgacgatcac atcaacaccc gcaccatgct gcaaaacatg gtgcgccacc
     3781 cgaacgcggg cgcagtgctg gtgattggtc tgggctgtga aaacaaccag gttgccgcat
     3841 tccgtgaaac gttgggcgat atcgatcctg aacgcgttca tttcatgatc tgccaacagc
     3901 aggatgatga gatcgaagcc gggatcgagc atttgcatca gctgtataac gtgatgcgca
     3961 acgataaacg cgagccaggc aaactcagcg aactgaagtt tggtctggag tgcggtggtt
     4021 ctgacggtct ttctggtatt actgctaacc cgatgctggg gcgtttctct gactacgtga
     4081 ttgctaacgg cggtactacc gtactgaccg aagtgccgga gatgtttggc gcagagcagt
     4141 tgctgatgga ccattgccgc gacgaagcaa cgtttgaaaa actggtcacc atggtcaacg
     4201 acttcaaaca gtactttatt gcccatgatc agccgatcta cgaaaaccca tcaccgggga
     4261 acaaagcggg cggtatcacc acgctggaag acaaatcact tggctgtacc cagaaagcgg
     4321 gttccagtgt cgtggttgac gtgctgcgtt acggcgagcg tctgaaaacg ccagggctga
     4381 acttgttaag tgcgccgggt aacgatgccg tagcgaccag cgccctggcg ggtgcgggct
     4441 gccatatggt gctgttcagt actggtcgcg gcacgccata cggtggattt gtgccgacgg
     4501 tgaaaatcgc caccaacagt gaactggcgg cgaagaaaaa acactggatc gactttgacg
     4561 cgggtcagct gatccacggt aaagcgatgc cgcagttgct ggaagaattt atcgacacca
     4621 tcgttgagtt tgccaacggt aagcaaacct gcaacgagcg taacgacttc cgcgaactgg
     4681 cgatcttcaa aagcggcgta acgctataaa gtcgaaatga gttgttaata aaaaacggcg
     4741 tttcataatg tgaagcgccg tttgcttttc catacctgct aaggatttat catgaccgcg
     4801 tattggctgg cccagggcgt gggtgtcatc gcctttctga ttggtatcac aacatttttc
     4861 aatcgtgacg aacgtcgctt caaaaagcag ctttcggtct atagcgccgt tattggcgta
     4921 catttttttc ttctgggcac ctatcccgct ggtgccagtg ccatccttaa tgccattcgt
     4981 acattgatta ccttacgcac gcgcagctta tgggtaatgg cgatttttat tgtgctgact
     5041 ggcggaattg gcctcgcgaa gttccatcat cctgtcgaac tattgccggt tatcggtacg
     5101 attgtcagta cctgggcgct gttccgctgt aaagggctca ccatgcgctg cgtgatgtgg
     5161 ttttcaacgt gttgctgggt gattcacaac ttctgggcgg ggtcgatagg cggcacgatg
     5221 attgagggta gttttctgct tatgaatggc ctgaatatca ttcgtttctg gcggatgcag
     5281 aaaaggggaa ttgatccgtt taaagtagag aaaacccctc ccgccataga cgaaaggggt
     5341 taaacaatta attacgcagg gcgctatttg ccagacgatc gtcttccgcc tggcaagctg
     5401 ccgcagtgaa cagcacgtca gttgaagagt tcagcgcggt ttcgcaagag tcctgcaata
     5461 cgccgataat aaagccgacg gcaaccacct gcatggcgat atcgttcgaa ataccgaaca
     5521 tattacaggc cagtgggatc agcagcaaag aaccccccgc cacgccggat gcgccacagg
     5581 cacacagaga agccactacg ctcaacagca gcgctgtggg cagatcgacc ggaataccca
     5641 gcgtattaac cgcagccagc gtcaacacgg taatggtgat tgctgcgccc gccatattga
     5701 tggtggctcc cagcggaata gaaactgaat aggtatcgcg atccagattc agcttttcac
     5761 acagcgccat attcaccgga atgttcgctg cagagctgcg ggtgaagaag gcatacacac
     5821 cgctttcgcg caggcacagc agcaccagcg ggaacgggtt acgacgaatt ttccaccaca
     5881 ccagcaatgg gttaaccacc agcgccacca gtaacataca gccaaccagc acgaccagca
     5941 gttgcgcgta gccccacagt gtggagaaac cggtggttgc cagggtagaa gaaaccagcc
     6001 caaaaatacc gaacggtgcg aagcgaatga ccagtttcac cataaaggta acggcattcg
     6061 acatatcatt gaccaggttt ttggtggtct cgttaccgtg acgcagtgcg aagccgaggc
     6121 cgatcgccca caccagaatc ccgatgtagt tacctttcag cagggcgtcg atggggttgg
     6181 aaaccatgct cattaccagt ccgcgcatca cttcgacaat gcctgacggc ggcgaaatat
     6241 cacccgcgct actggacaag tgcagggtag aagggaaggc aaagctgaag actactgcgg
     6301 ccagagctgc agagaaggtg cccagcagat agaggaacaa aataggacgg atattggttt
     6361 tctgcccgtg ctggtggtta gcaatagatg ccattaccag catcaacacc aggatggggg
     6421 caacagcttt cagtgcgccg acgaacaaag tacctaacag accaacagct tccgccgcgg
     6481 gttttgagat ccatgccaga agaatcccca gaacaaggcc gaccaggatt tgttttacca
     6541 ggctgccatg agccagacgc cggaatagcc ccggtgaacg ttgcgtagtc atttttcgat
     6601 cctttcattg tgttgtcgca catccctggt gcgttctgtc gacgcatctt tcaggatgta
     6661 acaaagtgtt tgcaaggtcg agtataagga aagttacagc ttcgggaagc gaaaaatgct
     6721 ggattttaaa gagaggtcat gttttgcaac ttaatggttg catttattta acataaacag
     6781 agggaaggac tggctactaa ggctgattag ataacatatg ggcaaaacta tttggaatat
     6841 gagaacggtt gcgctccttt aacgtgttat gccatataaa gtcactggcg
//
LOCUS       DAZOYF010000192         6760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZOYF010000192 DAZOYF010000000
VERSION     DAZOYF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.194"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            164..418
                     /gene="bssS"
                     /locus_tag="ACHUOF_003708"
     CDS             164..418
                     /gene="bssS"
                     /locus_tag="ACHUOF_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR0612714.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            533..1651
                     /gene="solA"
                     /locus_tag="ACHUOF_003709"
     CDS             533..1651
                     /gene="solA"
                     /locus_tag="ACHUOF_003709"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR0612715.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            1699..1812
                     /locus_tag="ACHUOF_003710"
     CDS             1699..1812
                     /locus_tag="ACHUOF_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFR0612716.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2073..2639
                     /locus_tag="ACHUOF_003711"
     CDS             2073..2639
                     /locus_tag="ACHUOF_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR0612717.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2643..3218
                     /locus_tag="ACHUOF_003712"
     CDS             2643..3218
                     /locus_tag="ACHUOF_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR0612718.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3260..4312)
                     /locus_tag="ACHUOF_003713"
     CDS             complement(3260..4312)
                     /locus_tag="ACHUOF_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR0612719.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4537..5457
                     /gene="lpxL"
                     /locus_tag="ACHUOF_003714"
     CDS             4537..5457
                     /gene="lpxL"
                     /locus_tag="ACHUOF_003714"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR0612720.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5629..5814
                     /locus_tag="ACHUOF_003715"
                     /pseudo
     CDS             5629..5814
                     /locus_tag="ACHUOF_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5850..>6760
                     /locus_tag="ACHUOF_003716"
     CDS             5850..>6760
                     /locus_tag="ACHUOF_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0612721.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT         1742 a         1617 c         1610 g         1791 t
ORIGIN      
        1 gctgttttat tgttcatata aatcttaaaa gcagaaattt atctgttccc ccctttaaaa
       61 attcggtgaa tacccttact tattggtata ctgaagttgc ttcagataaa aacgcattga
      121 acctcgaata acgttgtcta gtaacacgaa ttagggggcc atgatggaaa aaaataatga
      181 agtcattcag actcatccgc tcgtagggtg ggacatcagc accgttgata gctatgatgc
      241 gctgatgttg cgtttgcact accagacccc aaataagtcc gagcaggaag ggactgaagt
      301 tggtcagacg ctctggttaa ccactgatgt tgccagacaa tttatttcga tattagaagc
      361 aggaatcgcc aaaattgaat ccggtgattt cccggtaaac gagtatcggc gtcattaaca
      421 tgacatgacc atccacccaa aaggcacctc accggtgcct ttaccatttc tgggttgtat
      481 atctgcattc agttaattac tctgcttaaa ccatgagagc agagagaaaa aaatgaaata
      541 cgatctcatc attattggca gcggttccgt aggcgctgcc gccgggtatt atgcaacccg
      601 cgccggttta aacgtgctaa tgaccgacgc ccatatgcca ccgcatcaac acggcagcca
      661 ccacggcgat acgcgattaa ttcgccatgc ttatggtgaa ggcgaaaagt atgtcccgtt
      721 ggtcctccgc gcgcaaacgc tgtgggatga actttcccgc cacaacgaag atgatcccat
      781 ttttgtacgc tctggtgtca ttaacctcgg cccggctgac tccgcatttc tcgccaacgt
      841 cgcccacagc gccgaacagt ggcaactcaa cgttgaaaag ctcgatgcgc aagggattat
      901 ggcccgctgg ccagaaatac gcgtcccgga caactacatc ggcttatttg agactgattc
      961 cggttttttg cgcagcgaac tggcgattaa aacctggatc caactggcga aggaagcggg
     1021 ctgtgcgcaa ctgttcaact gcccggtcac cgcaattcgt catgacgatg atggcgtaac
     1081 tattgaaacg gttgacggtg agtatcaggc gaaaaaagcg attgtctgcg cgggaacatg
     1141 ggtaaaagac ctgctcccgg agctgcctgt ccagcccgta cgtaaagtat ttgcctggta
     1201 tcaggccgat ggccgctata gcgtgaagaa taaattcccg gcgtttaccg gtgaactgcc
     1261 caatggcgat caatattatg gttttccggc agaaaacgac gcgttgaaga ttggcaaaca
     1321 taacggaggc caggttatcc attcagcgga tgaacgtgtt ccgtttgcgg aagtggccag
     1381 tgatgggtca gaagccttcc cgttcttgcg caatgtattg ccgggtatcg gttgctgcct
     1441 gtacggcgct gcctgcacct atgataattc gcctgacgaa gattttatta tcgataccct
     1501 acccggccac gataatacac tgctcattac cggcctgagt gggcacggtt ttaaatttgc
     1561 gtcagtttta ggggaaatag ctgccgattt tgcgcaagac caaaaaagcg attttgattt
     1621 gacgccattc aggctttccc gcttccaata atcatcatta cggcctccag aatgaggccg
     1681 tttttacagg agtatttaat gcgtcggctg ttgcactatc tcatcaataa tattcgcgag
     1741 catctgatgc tttatctttt tctctgggga ttgttggcaa ttatggatct gatctatgtg
     1801 ttttattttt agttttccgc actgacataa actgacaaat atttaatctc cctttcattt
     1861 agcgtgaaaa attgaatgag attattcctg cttctacagc actcaccaga catctctgat
     1921 gctgaaccca ctaaacttat gaagattctt tcattattaa ttcaaaatta ttgaaataaa
     1981 aataaatatc attcaatttg ttatttttgt gttgcaattc attctccgac acgccatttt
     2041 ctttctctcg caaattcgta ttggatgcac ttatgtcatt cacaaatacc cctgaacgct
     2101 atggtgttat atctgcggcc ttccattggc taagcgcaat tattgtctac ggcatgtttg
     2161 ctttaggtct gtggatggtc acgctcagtt attacgatgg ctggtatcac aaagcacccg
     2221 aactgcataa aagcatcggt attttgttaa tgatggggct ggttattcgc gttctgtggc
     2281 gtgtcatatc tccaccgccg ggtccgctgc cgagttattc gcccatgact cgtcttgccg
     2341 caaaagctgg acatctcgca ttgtgcctat tgctgttcgc catcggcatc agcggctatc
     2401 tgatctcaac tgccgatggt aaaccgatca gcgtttttgg ctggtttgac gtccccgcga
     2461 cccttgccga cgctggcgca caggcagact ttgccggtgc cctgcatttt tggctcgcgt
     2521 ggagcgtcgt agtactgtct gttatgcacg gatttatggc cctgaagcat catttcatcg
     2581 ataaagacga cactctgaag cgcatgctgg gaaagtcgtc atctgactat ggagtataaa
     2641 aaatgaaaaa aagcctgctt ggtttaacct tcgcgtccct gatgttctct gccggttcag
     2701 cggttgccgc cgattacaaa attgacaaag aaggtcagca cgcctttgtt aacttccgca
     2761 tacagcatct tggctatagc tggttatatg gcacctttaa agatttcgac ggtactttta
     2821 cctttgatga aaaaaatccg gctgccgata aagtgaatgt gacaattaac accaccagcg
     2881 tcgatactaa ccacgccgaa cgcgataaac atcttcgcag tgcggatttt ctcaatacca
     2941 caaaatatcc acaggcaaca ttcacctcca ccagcgtgaa gaaagacggt gacgaactgg
     3001 atattaccgg cgatctgacg ctgaatggcg taaccaaacc cgtcacgctg gaagcgaaat
     3061 taattggtca gggcgacgac ccatggggcg gtaaacgtgc aggctttgag gccgaaggca
     3121 aaattaagct caaagacttc aatatcaaga cagatttagg tccagcttct caggaagtgg
     3181 atctgattat ttcagtggaa ggcgtacagc agaagtaaga gtgaaatgtt gccggatggc
     3241 acgcatccgg caatgatatt tattctgttg gatcaggaat acccagtgtt gtattcagac
     3301 gtccacgaga cttattaaag atcttattgc cattttcacg tcctgcccga cggcgtcgct
     3361 gttcttctgg cggtaacgcg ctttcttcgc agcaaatctc actacaacaa cctttgtatt
     3421 tttccgcgca tactggacac tgaataaaca gcaggtggca gccatcattt ttacagttgg
     3481 tatggctgtc gcacggcgcg ccgcactggt ggcaatgcgc gataatctca tccgatatac
     3541 gttcgcccat ccgctcgtca aaaacaaaat ttttgccaat aaaacgcacc ggcaagccct
     3601 gctcgcgcgc cttacgggcg tattcaataa ttccgccctc gatatgccag actttgttga
     3661 atccgttatg tttcatccag gcactggcct tttcacaacg aatgccgccg gtgcagtaca
     3721 tgacgatttt tttatcttta tgtgcctgca tcatctcgac tgcttttggc agctgctcac
     3781 ggaaggtatc tgccggaatt tcgagcgcgt tttcaaagtg ccccacttca tactcatagt
     3841 ggttacgcat gtcgataaat agtgcatcgg gatcgtcaag catggcgttc acttccgccg
     3901 cttgcagata ctcaccaaca ttgctggcat caaagtgagg atcgtcaata ccgtcggcaa
     3961 caatgcgatc gcgtaccttc atgcgcagta cccagaagga tttcccgtca tcatccaacg
     4021 cgatattcag gcgtaaaccc tctaaagccg ggtcgaaggc atagagctgc gcgcgaaatg
     4081 tttcaacatt gctcgcaggt acgctgattt gcgcgttaat gccctcatgc gccagataca
     4141 ctcgcccaaa aacattcagc gcggtaaaca gctgatataa agcgtcacgg gtcgccttag
     4201 gatcggcgat gtggaaatac ttgtaaaacg aaatggtggt tcgcggttcg ctctcagcca
     4261 acattttggc ttttaacgcg tcgttggaaa tgcggttgtg taacactggc atggtgtacg
     4321 gttcctgcga gatgggaaag taaaaatccg cggcatgata tagcaattat cgataattaa
     4381 catccacaca ttttacgcta catttgcgca ttaaaaatta tttgttattt acaagcgcgg
     4441 caatttcgcc cagtcttcag ccacaaattt tggttgcggg cgaaaaaatg cgacaataca
     4501 tacaattgcc cgtataggtt gaaaaacagg attgatatga cgaatctacc caagttctcc
     4561 accgcactgc ttcatccgcg ttattggtta acctggttgg gtattggcgt actttggttg
     4621 gtcgtgcaat tgccctaccc gattatctac cgcctcggtt gtggattagg aaaactggcg
     4681 ttacgtttta tgaaacgacg cgcaaaaatt gtgcatcgca acctggaact gtgcttcccg
     4741 gaaatgagcg aacaagaacg ccgtaaaatg gtggtgaaga atttcgaatc cgttggcatg
     4801 ggcctgatgg aaaccggtat ggcgtggttc tggccggacc gccgaatcgc ccgctggacg
     4861 gaagtgatcg gcatggaaca cattcgtgac gtgcaggcgc aaaaacgcgg catcctgtta
     4921 gttggcatcc attttctgac actggagctg ggcgcgcggc agtttggtat gcaggaaccg
     4981 gggattggcg tttaccgccc gaacgataat ccactgattg actggctaca aacctggggc
     5041 cgtttgcgct caaataaatc gatgctcgac cgcaaagatt taaaaggcat gattaaagcc
     5101 ctgaaaaaag gcgaagtggt ctggtacgca ccagatcatg attacggccc gcgctcaagc
     5161 gttttcgtcc cgttatttgc cgttgagcag gctgcgacca cgaccggaac ctggatgctg
     5221 gcacggatgt ccggcgcatg tctggtgccc ttcgttccac gccgtaagcc agatggcaaa
     5281 gggtatcaat tgattatgct gccaccagag tgttctccgc cactggatga tgccgaaact
     5341 accgccgcgt ggatgaacaa agtggtcgaa aaatgcatca tgatggcacc agagcagtat
     5401 atgtggttac accgtcgctt taaaacacgc ccggaaggcg ttccttcacg ctattaaatc
     5461 tcccatgccg gatgcttcag aatggcatcc ggcattacca cagcaaatcc ccctgattta
     5521 gcgataaaag ctctctggat tgcgccccct ggaagtcggg cgcataatta gtgtgtttat
     5581 cttttcttct tatgttcacc gcgcctggcg caccaacagc ggattgctat gtcaccctgt
     5641 gaaaatgaca cccctataaa ctggaaacga aacctgatcg tcgcctggct aggctgtttt
     5701 cttaccgggg ccgccttcag tctggtaatg cccttcttac ccctctacgt tgagcagctt
     5761 ggtgttaccg gccactccgc cctgaatatg tggtccggta ttgtcttcag tataatgggt
     5821 tcctaaacac atcattttag gtattgaata tgagacgggc aatcagctac atcagatttt
     5881 caagtgaacg acaactgaaa ggggactctg tacgccgaca atctaagctg gttactgact
     5941 ggcttgataa gaaccctgag ttttacttgg attcatcctt gagctttaaa gatttgggga
     6001 aaagtgcttt tagtggtaag cacctgaaag gtggtctagg tgacttttta accgctatcg
     6061 agaaagggct tgttaaagct ggtgatacgc tactaatcga gagtttagac cgtctgtcac
     6121 gtcaggatat tgatattgct tctgagctat tacgccgtat cttaagggct ggtgtagatg
     6181 tagtcacact gagcgatgga gaacattaca cacgagaaag tttaaaagac ccgttagccc
     6241 tgattaaaag cattctgata atgcagcggg cgcatgagga aagtttgaga aagtctgagc
     6301 gtgtccaagc tgcatggaac cgtaaaaaag aactcatctc cgaaggtatc aaggtatcta
     6361 ggcgttgtcc tgcatggtta aggctgaatg atgaccgtcg aacattcaca atcattcctg
     6421 ataaagtgga agttgttaaa cgagcatttg acctgagatt acagggttta agcttttggg
     6481 ctatcaccag aactttaaac gatgaaggtc acttgtcatt gaatcaatac acacccaaac
     6541 aaaaggggtg gtctgatact gctgtaaaga aactgctcag aaatcgtgct gtgattggat
     6601 gtttcacacc tgctggacgt gaagaagttc agggttacta ccccgcaata atcagtgagt
     6661 cactattcta tcgagtacag caactgaaca cagggcaata tggtagggct tctgtatcaa
     6721 gtaatccgct atcagtcaac ttgttcaggg gtatcatcaa
//
LOCUS       DAZOYF010000193         6604 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZOYF010000193 DAZOYF010000000
VERSION     DAZOYF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.195"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..1076
                     /locus_tag="ACHUOF_003717"
     CDS             <1..1076
                     /locus_tag="ACHUOF_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAPES1 domain-containing protein"
                     /protein_id="HFR0612722.1"
                     /translation="AEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTA
                     STDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPV
                     NVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVA
                     GQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYV
                     SYVHDLPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENI
                     SDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAIT
                     LHDAYKASDERLYVNKQNKNSRS"
     gene            1119..1622
                     /gene="yeaK"
                     /locus_tag="ACHUOF_003718"
     CDS             1119..1622
                     /gene="yeaK"
                     /locus_tag="ACHUOF_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR0612723.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1623..1727)
                     /gene="yoaI"
                     /locus_tag="ACHUOF_003719"
     CDS             complement(1623..1727)
                     /gene="yoaI"
                     /locus_tag="ACHUOF_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR0612724.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1897..2343
                     /locus_tag="ACHUOF_003720"
     CDS             1897..2343
                     /locus_tag="ACHUOF_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR0612725.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="ACHUOF_003721"
                     /pseudo
     CDS             complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="ACHUOF_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3215..4395
                     /gene="nimT"
                     /locus_tag="ACHUOF_003722"
                     /pseudo
     CDS             3215..4395
                     /gene="nimT"
                     /locus_tag="ACHUOF_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4450..4797
                     /locus_tag="ACHUOF_003723"
     CDS             4450..4797
                     /locus_tag="ACHUOF_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR0612726.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="ACHUOF_003724"
     CDS             complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="ACHUOF_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR0612727.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5256..6281
                     /gene="dgcP"
                     /locus_tag="ACHUOF_003725"
     CDS             5256..6281
                     /gene="dgcP"
                     /locus_tag="ACHUOF_003725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR0612728.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6314..6412
                     /locus_tag="ACHUOF_003726"
     CDS             6314..6412
                     /locus_tag="ACHUOF_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR0612729.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6415..6489
                     /gene="yoaJ"
                     /locus_tag="ACHUOF_003727"
     CDS             6415..6489
                     /gene="yoaJ"
                     /locus_tag="ACHUOF_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR0612730.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
BASE COUNT         1600 a         1601 c         1618 g         1785 t
ORIGIN      
        1 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
       61 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
      121 cactacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
      181 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
      241 tggccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
      301 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
      361 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
      421 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
      481 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
      541 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
      601 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
      661 acgtccatga cttacctggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
      721 tcacgtcttc gaggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
      781 tggtccggat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
      841 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
      901 tggtgcaatc cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagcaaa
      961 taaatgacac cctcggtcat caggaggggg atttagcgat tacgttacat gatgcgtata
     1021 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     1081 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     1141 gcgtgacgca tcagcagtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
     1201 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggt accgctctag
     1261 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     1321 tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc catataggcg
     1381 gattacgcgc ttcgttggcc agcccggcgg aagttgatga actcacaggc tgtgtcttcg
     1441 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     1501 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     1561 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
     1621 agctagccta tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatagcaaaa
     1681 aacgatgatg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     1741 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     1801 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcataaag
     1861 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     1921 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     1981 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     2041 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     2101 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     2161 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
     2221 cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc gctgtttcgc
     2281 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     2341 tagttaatcc cgcaatatat cggcctggcg tttgccccaa gcctttctta aacatagtaa
     2401 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     2461 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     2521 accccgtctc tttcactatc aggcgggcaa gattgcgttc actcatcgca aaaaagccag
     2581 cccattgccc caatgccccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
     2641 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
     2701 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     2761 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
     2821 gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc
     2881 acttgattgc tatgcttcac cccgcccggt atccaaaccg catattgtgg cggcaccatc
     2941 cacaaagcat tttccaccgt acaggtaatt gcaccatgta atgccagtat cagctgtcct
     3001 ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg
     3061 accgcggctt catggtgtcg gtgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctggcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcgcactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgccttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc ggcaagttct
     4201 gcgcatcagg cggcggcact ctcggggatg gcacaatccg tcgggtattt gctggcagcc
     4261 tgtgggccgc cgctaatggg taaaatacac gatgctaacg gtaactggtc cgtaccactg
     4321 atgggtgttg caatactttc actactgatg gcgattttcg gactttgcgc cgggagagac
     4381 aaagaaattc gctaatatcc ggtgctatag tgacgtgaca aatcttgcgt gaaagggaga
     4441 acaaacacga tgaatattca gtgcaaacgc gtttatgatc cggctgaaca gagcgatggt
     4501 tatcgcgtac tggtcgaccg cctctggccg cgcggtatca aaaaaaccga tttagccctt
     4561 gatgagtggg ataaagaaat cacgccgtca acggaactgc gcaaagcctt tcacggcgaa
     4621 gtcgtcgatt ttgcaacctt tcgcgagcaa tatcgcgcag aactggcgca acacgagcaa
     4681 gaaggaaagc ggctggcgga catcgccaaa aaacagccgc tgaccctgct ctacgcagca
     4741 aaaaacacca cgcagaacca tgcgctggtg ctggccgact ggctacgtag cttgtgattt
     4801 tagtacggca tccggcggtt atttttcacc agccggatgg tcccgtcgcc acaatgccca
     4861 ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg ctgacccctt gcgctgtctg
     4921 cactggaatg agcgtcccgc ccttctgctg gcaatagacc gacgccggat ttgccatacc
     4981 aatctgcggc ggtttaggtg cttctggctg agaaggggtt gaacaaccag ccaggaccag
     5041 caagcaaggc agaacaaaac tgataatttt catttattga tctcacatat ttatccaagc
     5101 ttagagtatc gcggtatcgt tttgttttgc agcactattt ttattacatt cactcaaaac
     5161 atattacgtc ttgtttcatc tttgttgatg atgttttatc atgcctgcaa agattaaata
     5221 atcagcactt acccgccgta tcctggagtt gttccgtgtc agatcagatt atcgcccgcg
     5281 tctcgcaatc ccttgccaaa gaacagtcac tggaaagtct ggtccgacag cttctggaga
     5341 tgctggaaat ggtcactgat atggaatcaa cctacctgac caaagtggat gtcgaagcgc
     5401 gcctgcagca tattatgttt gcccgtaaca gccagaaaat gcacatcccg gagaatttta
     5461 ccgtcttgtg ggattactcg ttatgcaaac gcgccattga tgaaaactgc tttttcagcg
     5521 atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg caatcttggc atcaccacat
     5581 ttctgagcac gccaattcac ttaccggatg gatcattcta tggcacgctt tgcgccgcca
     5641 gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt tttacagtta ttcgccggac
     5701 tgattgcaca atatattcaa aaagaggcac tggttgaaca gctgcacgaa gccaatgctg
     5761 cgctgattgc gcaatcgtat accgactcgt taaccgggct accgaatcgg cgggcgattt
     5821 ttgaaaatct gacgacgctg ttttccctcg cccggcatct taaccataag ataatgatcg
     5881 cgtttatcga tctggataac ttcaaattaa tcaatgatcg ttttggtcat aatagtggcg
     5941 atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta
     6001 ttggtcgtct cggtggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata
     6061 tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac ttaggtgatg
     6121 ttgatttgta ttatcccggt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg
     6181 atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac
     6241 agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc acatcctttt
     6301 gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttca ttacagcggt
     6361 cgtattttta gcatggtttt ttattggctg ctatgctgcc ccgggagcat aaagatgaaa
     6421 aaaacaacga ttattatgat gggtgtggcg attattgtcg tactcggcac tgagctggga
     6481 tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat
     6541 ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca ccaataacgg
     6601 ctga
//
LOCUS       DAZOYF010000194         6521 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZOYF010000194 DAZOYF010000000
VERSION     DAZOYF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.196"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..115
                     /locus_tag="ACHUOF_003728"
     CDS             <1..115
                     /locus_tag="ACHUOF_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612731.1"
                     /translation="LDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            439..1827
                     /gene="narU"
                     /locus_tag="ACHUOF_003729"
     CDS             439..1827
                     /gene="narU"
                     /locus_tag="ACHUOF_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR0612732.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1909..5649
                     /gene="narZ"
                     /locus_tag="ACHUOF_003730"
                     /pseudo
     CDS             1909..5649
                     /gene="narZ"
                     /locus_tag="ACHUOF_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5646..>6521
                     /gene="narH"
                     /locus_tag="ACHUOF_003731"
     CDS             5646..>6521
                     /gene="narH"
                     /locus_tag="ACHUOF_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0612733.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1558 a         1714 c         1690 g         1559 t
ORIGIN      
        1 cttagatgaa ataaacaata cttactataa taacgattat tacaatcagg caataagaga
       61 ggaaccgata gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat
      121 aaatcatcac taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc
      181 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt
      241 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      301 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
      361 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
      421 gtgcatatga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
      481 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
      541 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
      601 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
      661 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
      721 cctatattcg gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc
      781 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
      841 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
      901 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
      961 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1021 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1081 tggatttggg taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat
     1141 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
     1201 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1261 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1321 ggcccattta ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     1381 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     1441 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     1501 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     1561 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga
     1621 gaagccgtca ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc
     1681 ggctttttta ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc
     1741 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     1801 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat
     1861 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1921 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     1981 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2041 atcgtgcgtt ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt
     2101 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2161 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2221 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2281 gccctcaaac aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa
     2341 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2401 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2461 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg
     2521 cgttatctgt cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg
     2581 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg
     2641 tataactcca gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac
     2701 gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2761 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     2821 gccctggcga tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2881 gactacttta tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag
     2941 cctcgcgacg atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat
     3001 ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa
     3061 ttggtagtgc cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3121 gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3181 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3241 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3301 ggcaacacct gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3361 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     3421 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     3481 tttgccgata ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt
     3541 aaccactggt atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc
     3601 ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc
     3661 ccacaaaccg gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag
     3721 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3781 gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat
     3841 ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3901 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3961 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4021 catccgcgta atttgtttgt ctggcgctct aacctgcttg actcctccgg caaaggccac
     4081 gagtatatac agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4141 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4201 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4261 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca
     4321 tttattcatc cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4381 atctacaaag gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa
     4441 accgacgtgg tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt
     4501 gaagtgctcg actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat
     4561 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4621 ttgatggaca aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4681 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     4741 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     4801 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat
     4861 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4921 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg
     4981 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5041 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5101 atcgacaccc gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa
     5161 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5221 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc
     5281 cgagaactga ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5341 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5401 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5461 aactcggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     5521 gcctggggct ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc
     5581 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5641 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     5701 gccatacctg ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg
     5761 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5821 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg
     5881 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg
     5941 atgattacta cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca
     6001 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     6061 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     6121 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     6181 acctgccgcg cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg
     6241 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     6301 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     6361 gcaagtcaga aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     6421 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     6481 gcatcgagga agcgtcgagc accgagcgcg aagttgacct c
//
LOCUS       DAZOYF010000195         6395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZOYF010000195 DAZOYF010000000
VERSION     DAZOYF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.197"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..856)
                     /locus_tag="ACHUOF_003732"
     CDS             complement(<1..856)
                     /locus_tag="ACHUOF_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR0612734.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNHVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNV"
     gene            964..1242
                     /locus_tag="ACHUOF_003733"
     CDS             964..1242
                     /locus_tag="ACHUOF_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612735.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACHUOF_003734"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACHUOF_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR0612736.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="ACHUOF_003735"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="ACHUOF_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR0612737.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="ACHUOF_003736"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="ACHUOF_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR0612738.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="ACHUOF_003737"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="ACHUOF_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR0612739.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..4880
                     /locus_tag="ACHUOF_003738"
                     /pseudo
     CDS             4234..4880
                     /locus_tag="ACHUOF_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4922..5452)
                     /locus_tag="ACHUOF_003739"
     CDS             complement(4922..5452)
                     /locus_tag="ACHUOF_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR0612740.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5608..6174)
                     /gene="slp"
                     /locus_tag="ACHUOF_003740"
     CDS             complement(5608..6174)
                     /gene="slp"
                     /locus_tag="ACHUOF_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR0612741.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         1857 a         1332 c         1376 g         1830 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtggt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg
     4381 aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt
     4441 gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac
     4501 caaaatattg taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg
     4561 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     4621 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     4681 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     4741 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     4801 ttcatgccac aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     4861 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     4921 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     4981 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     5041 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     5101 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     5161 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     5221 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     5281 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     5341 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     5401 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     5461 cctggtgcgg acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt
     5521 ataataataa tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg
     5581 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
     5641 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
     5701 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     5761 catattcact tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg
     5821 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     5881 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     5941 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     6001 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     6061 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     6121 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
     6181 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
     6241 tgaggtgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag
     6301 ttcaattccg ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt
     6361 gttttaaaat tacataaaat ctatttttat tcaat
//
LOCUS       DAZOYF010000196         6374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZOYF010000196 DAZOYF010000000
VERSION     DAZOYF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.198"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..378
                     /locus_tag="ACHUOF_003741"
     CDS             <1..378
                     /locus_tag="ACHUOF_003741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612742.1"
                     /translation="IIRWLLKKRTNGSIRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHUOF_003742"
     CDS             complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHUOF_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR0612743.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHUOF_003743"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHUOF_003743"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR0612744.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="ACHUOF_003744"
     CDS             complement(1787..2476)
                     /locus_tag="ACHUOF_003744"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR0612745.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHUOF_003745"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHUOF_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR0612746.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHUOF_003746"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHUOF_003746"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR0612747.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHUOF_003747"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHUOF_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR0612748.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1621 a         1716 c         1577 g         1460 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagta tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcagaatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc ttccaactta ctgatttagt gtatgatggt gattttaagg
     6361 tgcttgcgtg gctt
//
LOCUS       DAZOYF010000197         6356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZOYF010000197 DAZOYF010000000
VERSION     DAZOYF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.199"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUOF_003748"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUOF_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0612749.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHUOF_003749"
     CDS             2219..2767
                     /locus_tag="ACHUOF_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR0612750.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUOF_003750"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUOF_003750"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR0612751.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHUOF_003751"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHUOF_003751"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR0612752.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHUOF_003752"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHUOF_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR0612753.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1610 a         1553 c         1611 g         1582 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaac
//
LOCUS       DAZOYF010000198         6308 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZOYF010000198 DAZOYF010000000
VERSION     DAZOYF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.200"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..671
                     /locus_tag="ACHUOF_003753"
     CDS             <1..671
                     /locus_tag="ACHUOF_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR0612754.1"
                     /translation="RGLYLFYTRLPYLAMILCGLIQATSIILTFNGTRSHAPLGFSFD
                     VMSSIISSNVFLFSFVPWNIANAGWDNHLLSYTLSTLVITLCVFIYIKGNWQMKVFAT
                     LPILIVAFSMAKPQLADSVPQLPTLATGNGSRYFVNIHIAIFSLICVCLFQCIKNKTL
                     KIFFKIYVSVILIAMIKLNFFITPLPDMNWSQGAELINKAKHGEAVSFTVLPPWLTLD
                     LIKK"
     gene            complement(738..2450)
                     /locus_tag="ACHUOF_003754"
     CDS             complement(738..2450)
                     /locus_tag="ACHUOF_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-binding domain-containing protein"
                     /protein_id="HFR0612755.1"
                     /translation="MPEQLYNVVVSQPSQLFTLARSFKANANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIVINAAGYPVYNGQIAKFVTEQGHSMAVYDAYGSQQFY
                     FPNLLKYDPDQLKQLLQSDEGATYIGTNSGKNLQDELFAIKNEIYQDPLTSWPFGGTL
                     KIKQGVYDVTTPLVLNYDDYPADFVGAPGVRCFYSGENMAETILECRATDFNVKMLGD
                     AAFNSQRLAAYDYIGNMTLKGNPGSYGMLVQNKAHTRLENIVICRHPDGEGLRTDSML
                     TSDMTNVYLQSNRIGWRATNSVNHSELNAITANRLTCSQNSEWGILGDRWGAGTTINS
                     LTCEGNGTQGDSSTGGAQLAINGLNGSCALVLNNPYFEANAGGADLAIDNTGTRPVTV
                     VINGGNFHRVSSARYTHTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSADRPYWITGA
                     NCEVVDIGCVFTETTSKATSASALSVTRSGKINANGSIDVATGVSSVNVVATGVYEVS
                     FSHPLAAVASGYIVQITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSI
                     TRLL"
     gene            2515..2973
                     /locus_tag="ACHUOF_003755"
     CDS             2515..2973
                     /locus_tag="ACHUOF_003755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0612756.1"
                     /translation="MEVLYYNKETGIFTWKKKLSARGAVGKVAGTISYGYNAINIDGV
                     RYFAHRLAWLYVYGEWPKQEIDHIDRNRRNNAISNLRDVSRVVNALNVGPRNSNAGIK
                     GVTFCQARNQWQAQINVSGKNITLGRFNTIDEAAIAYKAANMVADHLLSR"
     gene            3105..3365
                     /locus_tag="ACHUOF_003756"
     CDS             3105..3365
                     /locus_tag="ACHUOF_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFR0612757.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LDKQNSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSS"
     gene            complement(3455..5452)
                     /locus_tag="ACHUOF_003757"
     CDS             complement(3455..5452)
                     /locus_tag="ACHUOF_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR0612758.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGENAEQ
                     AKQAFYAAYPLPSVQPVETQQPVAQQQPQQSGFMSDLGEAVKETGRGLVQAGVNVANI
                     PASVADAVTSAAAWAGGKLGIGDGTYQPAPRVTTHGLEQDFGLQQGALTPQTTEGRVF
                     AEALPYLTPAGVERAATQAPTLAGRIAQGATRLLAENAVGSLAANSAKDDAEALATDL
                     GVGVLAGGAINAAGRGLGAAYRGVRGAIAPEAQQAIRFAEREGVPLHTTDLLQPTSRV
                     GKMAQTTAENIPLAGTSGMRATQQEARSQLVQRFADKFGEYDPAVVIDSLKAKTSGIR
                     RAAGNRLEQVQNAMAGVNIQPARAIQQIDTEISNLQKLGKVADNETISKLQSYRDELV
                     RNAGPEGPVNLDLKQLSDLRSQFRMDVKGERPVLPNRSDAAIQRVYKAMTDDINGAIG
                     QNLGNDTLRKYQQANAVYADEAAKLKNTRLKNVLMKGDLTPEVVNNMLFSKNKSEIKT
                     LYNSVGRVGRVQMRNGIIGKAMEKSGGSPDQFLRQLNILQNQTGITFKGQDAAYLKGL
                     KNYLQSTQQAAKAAVTTPTGQQTIPFIIGYGTAMNPATTGAAVSYGLLTRAYESEPFR
                     NAMLRMANTPRGSTAFEKAMQQAQKAINALTQGAKSDALSE"
     gene            complement(5452..>6308)
                     /locus_tag="ACHUOF_003758"
     CDS             complement(5452..>6308)
                     /locus_tag="ACHUOF_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR0612759.1"
                     /translation="ELTRVGVDPNNVAQMYQQNPSGFGEFVDHLGMAALGPIDYFNVQ
                     DKMAGREIDRGRLAETIRSNQAGEALTARGQDIQIRGQNISAQNAALSREIQRAELQE
                     KALDRQIARESNQLKLEELKQKQADVRQKAEIARADRQAAAQGAVDTFSTALDSLNEI
                     EQSPGLSKAVGIRSAFPTVPGSDAANFEARLDTFKAQTFLPMVQSLKGMGALSDAEGK
                     KLSDAVGALSPKMSEKAFRDSIGKIRNQLESKLSTVKKQFDYQEPVQNTPGQQSPAGS
                     NFSSLWGD"
BASE COUNT         1641 a         1504 c         1361 g         1802 t
ORIGIN      
        1 taagaggttt atacttattt tatactcgac tgccatattt ggcaatgatc ttgtgtgggc
       61 tgatacaggc aacatcaata attcttacat ttaatggaac aagatcacat gctccattag
      121 gattcagttt tgatgttatg tcatctataa tatcatctaa tgtatttctt ttttcatttg
      181 tcccttggaa tatcgctaat gctggatggg acaatcacct tttatcgtac acactatcaa
      241 ctttagtaat tacattatgt gttttcattt atattaaagg aaattggcaa atgaaagtgt
      301 ttgccaccct tccaatactt attgtcgcat tttctatggc aaaacctcaa ctggcagatt
      361 ctgttcctca actgccaaca cttgcaaccg gaaacgggtc aaggtatttt gtaaatattc
      421 atattgcaat tttttcatta atatgcgttt gtttgtttca atgcataaag aataaaacct
      481 taaaaatatt ctttaaaatc tatgtttcag tcatccttat tgcaatgata aaattaaact
      541 tttttataac ccctcttcct gacatgaact ggtctcaagg agcagaatta ataaataaag
      601 caaaacatgg agaagccgtc tcatttacag tgctccctcc atggttaact cttgatttaa
      661 taaaaaaata aaacttcaca ttaataaata acaatgaatc cgcctcccta cgtattcccg
      721 tagggaggct gtcgtgttta tagtagcctg gtaatgctaa atgcaaacga actagagatt
      781 cctgctccgc taagcgtatt gcgcagggtc accctaaatg tatctacgcc aatataagta
      841 acatcacaac taactgaatc aggcgcagat ataggtgtta tctgcacaat ataaccgcta
      901 gcaactgctg cgagcggatg agaaaagcta acctcataca cacctgtagc aaccacgtta
      961 accgatgaaa caccagtagc aacgtcgata ctaccattgg cgttaatttt tcccgagcgt
     1021 gtgactgaca atgcagaagc cgatgttgcc ttactggttg tttctgtaaa aacacaccca
     1081 atgtcaacta cctcgcaatt agctcctgtt atccagtatg gtcgatctgc acttggctga
     1141 taatcccctg cgctctggaa cgttgtgccg ttgagtatga ctgtaacttt tcccccacct
     1201 gagcttgtta cttgaatatt agtgtgcgtg tatcttgcac tgctcacgcg atgaaaattt
     1261 ccaccgttta taaccacagt tacagggcgc gtcccagtat tatctatagc aaggtctgcc
     1321 cccccggcat tagcctcaaa gtatgggttg tttaatacca aagcgcacga accgttaagg
     1381 ccatttatgg ctaattgcgc accacctgta ctggaatccc cctgagtacc atttccttca
     1441 caagtcaggc tattgatagt tgtaccagca ccccagcggt ccccgaggat accccattca
     1501 ctgttctgac tgcacgtaag cctatttgca gttatagcgt tcagttcaga atgatttacg
     1561 gagttggtag cacgccaacc gatacgatta ctctgcaagt acacgttcgt catgtcgctc
     1621 gtgagcatcg agtcagtgcg taacccttcc ccgtcagggt ggcggcagat aacaatgttt
     1681 tcaagacgag tatgtgcttt attctgcacc agcataccgt aacttccggg attccctttc
     1741 agcgtcatgt tgccgatgta gtcatatgca gccaatctct gactgttaaa cgctgcgtca
     1801 cctagcattt ttacgttaaa atcagtagct ctacattcca gtattgtctc agccatgttc
     1861 tcgcctgagt aaaaacagcg gacacccgga gcacctacaa agtcggcagg gtaatcatca
     1921 taattaagta ccagaggagt ggtaacatca tacacccctt gctttatttt taatgttcca
     1981 ccgaaaggcc acgatgtaag cgggtcttga tatatttcat tttttattgc aaatagctca
     2041 tcttgaagat ttttcccgct attagtgcca atgtaagtag ccccttcatc agattgaagt
     2101 aactgcttta actgatccgg gtcatacttc agcaaattcg gaaaatagaa ctgttgcgaa
     2161 ccatacgcat catatacagc cattgaatgg ccttgctcag ttacaaattt ggcaatctgc
     2221 ccgttgtaca caggatatcc ggcagcattg ataacaattg gctgagaaac agggacgtga
     2281 gatccgtctt cattctctac ataaacctga atctggtttt caggatttac cgggtcagtg
     2341 tcaattttcc cgatataaat ttttccattg gcatttgctt taaacgagcg agctaaagta
     2401 aataactgac ttggttgtga aacaacaacg ttgtataatt gttctggcat aataaacatt
     2461 ccggagaaaa taatgagtaa gagacgtcac gaggtagtaa ctcgggagcg ccttatggag
     2521 gttctctatt acaataaaga aacaggaatt ttcacttgga aaaagaaatt aagcgccaga
     2581 ggtgctgttg ggaaagttgc tggcacaatt tcttatggat ataacgcaat taatattgat
     2641 ggcgtaagat attttgcaca tagacttgcc tggctgtatg tatatggcga atggcccaaa
     2701 caagagattg accacattga tagaaacaga agaaataacg caatctcaaa ccttagggat
     2761 gtcagcagag tcgtaaatgc attaaatgtc ggacctcgaa atagcaatgc tggaatcaag
     2821 ggggtaactt tctgtcaagc cagaaatcaa tggcaagctc agataaatgt ctcaggtaaa
     2881 aacataaccc ttgggagatt caacaccatt gatgaagcgg ctatagctta taaggcagca
     2941 aacatggttg ctgaccatct gttgagtagg tgatatctga catttcattg ctccagacga
     3001 atgatatgat gcaaccatga tgtgattgca taccgaaatg gtactattga gtatttatcc
     3061 agtaagttac gatgccattc cacccaattg gtgaggcatc aaggatgtac agcaaatacg
     3121 acgaggcgca gtttcacttg agacttccgc atgaactcca cgcgaaaatt aagcagcgtg
     3181 cgaagatgaa taacaggtct ctgaactcag agataattgc agcgattgaa gaatcactgg
     3241 ataaacaaaa ctctgcatca gtttacattg acgatgcaga gcgtatggca gaacaacaat
     3301 ccgatatggt taagaaaatt gtctttgata cgctcaaaga gctatataaa aaagacagca
     3361 gctaaccatc agttacggag gatttatgca aagagatatg ctgaatattg cgttctacat
     3421 atttggtttt tgcacgttcc tggtgtttgc gaagctattc tgacaacgca tcagacttgg
     3481 caccctgagt cagggcgtta atggcctttt gcgcctgctg catggctttc tcaaacgctg
     3541 ttgatccgcg tggggtattt gccattcgga gcattgcatt tctgaatggc tcgctctcat
     3601 aggcgcgagt aagaagtccg tagcttaccg ctgcgccagt tgtcgccggg ttcattgccg
     3661 tcccataccc gataatgaac gggatggttt gctgccctgt tggtgttgtt actgccgctt
     3721 ttgctgcctg ctgagtggac tgaaggtaat ttttcaatcc tttcagataa gcagcgtcct
     3781 gccccttaaa tgtgatgcca gtctggtttt gcaggatgtt aagctgccga aggaactggt
     3841 caggggatcc gccagatttc tccatcgcct ttccaatgat gccattgcgc atttgcaccc
     3901 tgccaacacg accaactgag ttatacagcg tcttaatttc cgatttgttc ttgctgaata
     3961 gcatgttgtt gacaacttcc ggcgtcaggt cgcctttcat gagaacattc ttcagcctgg
     4021 tattctttag tttcgccgct tcgtcagcgt agacggcatt ggcctgctga tatttacgga
     4081 gagtatcgtt gccaagattc tgaccaatgg caccattgat atcgtcggtc atcgccttgt
     4141 aaacgcgctg aatggcagca tcggaacggt ttggtaacac tggtcgctca cccttcacgt
     4201 ccattctgaa ctgactgcgc aggtcgctta attgcttcaa atccagattt accggacctt
     4261 cagggccagc attgcgaaca agctcatcac gatatgactg aagttttgaa attgtctcgt
     4321 tatcagccac cttaccaagc ttctgcaggt tagatatttc tgtatcaatc tgctgaattg
     4381 ctcgcgcagg ctgaatgttt actccagcca tagcattctg aacctgctcc agtcgattac
     4441 cggcggcacg acgaattcct gatgttttcg ctttaaggct gtcaataaca accgctggat
     4501 catactcccc gaatttatca gcaaatctct gcaccaactg gcttctcgct tcctgttgcg
     4561 ttgctctcat tccgcttgtg ccagccagag ggatattttc tgctgtagtc tgcgccattt
     4621 ttccgacgcg ggaagttggt tgtaacaggt ctgtggtgtg cagaggaact ccttcacgct
     4681 ctgcaaatct gatagcctgc tgagcttctg gcgcgatagc accacgaacg ccacgataag
     4741 cagcacctaa tccacgtccg gcagcgttaa tagcaccgcc agcaagtaca ccaacgccta
     4801 aatcggtggc gagtgcttcc gcatcatctt tcgcactatt tgcagcaagt gatccaactg
     4861 cgttttcagc gagaaggcga gttgcaccct gagcaattcg accagcaagt gttggtgcct
     4921 gtgttgccgc tctctcaacg ccggcaggag tgaggtaagg caatgcttca gcaaataccc
     4981 tgccctctgt cgtttgtgga gtcagcgcac cttgctgaag gccaaagtcc tgctctaatc
     5041 catgcgttgt tactcgtggc gctggttgat atgttccatc gccaatgccg agtttaccgc
     5101 cagcccatgc agccgcgctt gttacagcat cggcaaccga tgcaggtatg tttgccacgt
     5161 tcacgccagc ctgcaccagt ccgcgaccag tctcttttac tgcttcgcca agatcagaca
     5221 taaatccact ttgctgtggt tgttgctgtg ctactggttg ctgtgtctcc actggctgca
     5281 cagatggcaa tggataggca gcatagaaag cttgcttagc ctgctctgca ttttctccgg
     5341 cttgcggggc aacgacttca ttgaagtatt gctcctgagc ctgcgctttt tgttctggtg
     5401 ctaacgcctg atactgtgga gatgcgataa catctttcca tgctttagcc attaatcacc
     5461 ccatagtgaa gaaaaattac tgccagcagg agattgttgc cctggcgtat tctgcaccgg
     5521 ctcctggtaa tcaaactgtt ttttaacagt gctcaacttg ctttcaagct gatttcgaat
     5581 ctttccgata gagtcacgaa aagccttttc actcattttg ggacttagag caccaaccgc
     5641 atcggataat tttttaccct cagcatctga aagagccccc atccccttca gggactgcac
     5701 catagggagg aaagtttgag ctttaaaggt gtcgagtctt gcttcaaagt tagccgcatc
     5761 agatccagga actgtcggaa acgctgagcg aataccaact gcttttgaaa gtccagggct
     5821 ttgctctatc tcgttgagag aatcaagcgc agtgctgaac gtatcaacag cgccctgagc
     5881 ggcggcctgt ctgtcagcac gagctatttc agccttttgc cgaacatctg cctgtttctg
     5941 ctttagctct tcaagcttta actgattgct ttctctggct atctgtctgt ccagagcctt
     6001 ttcttgtaat tctgctcttt gtatttcgcg ggaaagagca gcattctgtg cgctgatgtt
     6061 ctggccacgt atctggatgt cctgacctcg tgctgttagt gcctcgccag cctgattgct
     6121 gcggattgtc tctgccagcc tacctcggtc aatctcacga ccagccatct tgtcctgaac
     6181 attgaagtaa tcaatcggac caagagcagc catcccaagg tgatcaacaa actcaccaaa
     6241 tcctgaagga ttctgctgat acatctgagc aacgttgtta ggatcaacgc cgacgcgagt
     6301 cagttcct
//
LOCUS       DAZOYF010000199         6183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZOYF010000199 DAZOYF010000000
VERSION     DAZOYF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.201"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHUOF_003759"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHUOF_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR0612760.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="ACHUOF_003760"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="ACHUOF_003760"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR0612761.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="ACHUOF_003761"
     CDS             complement(3736..4092)
                     /locus_tag="ACHUOF_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0612762.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="ACHUOF_003762"
     CDS             complement(4096..4512)
                     /locus_tag="ACHUOF_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR0612763.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHUOF_003763"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHUOF_003763"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR0612764.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="ACHUOF_003764"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="ACHUOF_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1536 a         1550 c         1558 g         1539 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 ata
//
LOCUS       DAZOYF010000200         6098 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZOYF010000200 DAZOYF010000000
VERSION     DAZOYF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.202"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..84
                     /locus_tag="ACHUOF_003765"
     CDS             <1..84
                     /locus_tag="ACHUOF_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612765.1"
                     /translation="YESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="ACHUOF_003766"
                     /pseudo
     CDS             complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="ACHUOF_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(1611..2201)
                     /locus_tag="ACHUOF_003767"
     CDS             complement(1611..2201)
                     /locus_tag="ACHUOF_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0612766.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="ACHUOF_003768"
     CDS             complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="ACHUOF_003768"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR0612767.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3179..3715
                     /locus_tag="ACHUOF_003769"
     CDS             3179..3715
                     /locus_tag="ACHUOF_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR0612768.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3756..4616)
                     /locus_tag="ACHUOF_003770"
     CDS             complement(3756..4616)
                     /locus_tag="ACHUOF_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR0612769.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="ACHUOF_003771"
     CDS             complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="ACHUOF_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR0612770.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="ACHUOF_003772"
     CDS             complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="ACHUOF_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR0612771.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1563 a         1543 c         1512 g         1480 t
ORIGIN      
        1 tatgagtcag tatgcatcgc cgcgatggaa catttaaaaa gtaaagcaac ggcccctata
       61 caaaacggac cgttgccagc ataagaatta acggtgtgcc agttcggcgt cgtcttcgct
      121 atccagaatg gctttatcgg tttgcttcag ccactggctg gtcagcgtgc cagctgtcat
      181 cgagccacta acgtttagcg ccgtacggcc catgtcgata agcggttcaa cggagattaa
      241 cagcgccacc agggttactg gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt
      301 tgcaccacca ccgacaccgg caacgcctgc ggaactaacg gtaacaatac cgaccagcgt
      361 cgcaatccac atcgggtcca gcgggttaat gccaaccgta ggcgcaacca tcaccgcagc
      421 attgccggat acaaaccggc gcagccgttc tgaccaatgg ttgcaccgaa agaggcggcg
      481 aaactggcga tggattcagg aacgcccaga cgacgcgttt gtgcttccac attcagtggg
      541 atagacgcag cactggaacg gctggtaaag gcaaacgtca gcacaggcca taccttacgg
      601 aagtacttca gcggactcac gccattaatg cccagcagaa tgccatgcac agcaaacata
      661 atcagcagac cgaggtagga cgcgacaacg aaacttccca gtttgatgat gtcttgcaag
      721 ttagaacctg caaccacttt ggtcattaga gccagaacgc cgtaaggggt caactgcatg
      781 acaaggcgaa ccagtttcat cacccagctt tgtagggtat cgatagcggt taagacgcgt
      841 tcccctttcg gcgcatcatc cttcagcagt ttcaatgcag ctacgccgag gaatgcggca
      901 aaaattacca cgctgataat tgacgtcgga ttggctccgg taagatcggc aaacgggttt
      961 ttcgggataa aggacaagac cagctgcgga acgctctgat cagagacttt accaacatag
     1021 ttactttcaa tggcgttcag acgtgcagtt tctgcaccac cctgaaccag accttcagcc
     1081 gtcaaaccaa acaggttggt gaccagcaca ccgaccagcg ccgcaatcag cgtggtaaac
     1141 aaaagcgtac cgatggtcag aaaactgatt ttgcctaact gagatgcgtt atgcagacgg
     1201 gcaaccgcgc tcagaataga ggcgaagact aacggcataa cgatcatttg cagcagttga
     1261 acatagccgt taccaacgat gttaaaccac tgtacagaat ctttaagtac ctggctgtca
     1321 gaaccataaa tggtatgcag ggcaaggcca aaaaccacac ccatcaccag acccaccagc
     1381 acttttttcg ccagactcca ctgtttatgg cgggtctgag ccagcgcaaa cagcagtaca
     1441 acgaacacca cgatgttcgc aattaatgga aagttcatcc ccgttctcct gatcttatta
     1501 tttctatcac tgcgtagcag tcatagtgtt ggcgcaagat taacagaact gtgagctggc
     1561 tcttatattc aaatggaatt gcttattcca gaaaggtgat ttttgccttt ttagtattca
     1621 acctgatgct taataagccg atgaaactgg atctgcaagg tattgatcat ttgcgatgac
     1681 caacgaacag cgctgttctc gggtaatgaa tggggcatcc ataccgacca gacaaataat
     1741 gccatgcaga taaaacgttc cagttggttg cccttttggg gaaccaggat aggcaagcgg
     1801 aaacgccagc ggcatggcca gagcagggga acaccggcgg gtgtcagcat atcggcaagt
     1861 atgtggctca aataacccag caccattcct tgtagcgcat cagccggaat gaaccagcct
     1921 tccggaacct taaggtaaaa ggttgccagc agcgcaaata ccgccagcag actgtgggta
     1981 aaaccacgat gcccaaaagc gcgggcgatc ggttttgata tccattttaa tcgctgccca
     2041 agaaacgact ttgggtgatc gatgtccggt aacaaacacg tcaggattgc ggaagggaca
     2101 atatgccacc agtcaccctg tgccagcacg ggcgtcagct cggcattttt ggcaaatacc
     2161 gcacaagcaa tagaaaagag aaggtgacct tccgccgtca tgataaaact cactaaactg
     2221 tcgatacgta cagtatagga tttttataca gtgcacggaa gtggtgacgg tgtaactctt
     2281 tctcttaaat tgacgattaa tccgtcagtt gaatgcaaat tcagataaag aaccaggggc
     2341 agcgtgatca ctgcccctgg tcattaaccg agaaggtctt ttgcggtgag ttctgtcagc
     2401 gatgagagtt tgacgtttgc taatacaaaa cgtggatcat tttgcgcttc tggcgcagga
     2461 acgacgatgg aacgcatgcg ggctgcttta gaagcgatca tgccatttac cgagtcttcc
     2521 agcgccacgc aggtcagtgg gtcaacgccc agttttgctg cgcagtcgag atatacttgc
     2581 ggatgcggct tgctgtaagg cagtttttca gccgaggcga gggcatcgaa actgtcgcgt
     2641 aagtcaaaca tggtcaacac tttttccagc atatgtagtg gtgacgcgga ggccagtccc
     2701 accaataaac cttgttcttt gcataacgca acagcttcgc gcacgcctgg taataatgga
     2761 cgtgtctctt caaccagtga aatggcacgg gcaataaccc gttctactac ttcctgacgg
     2821 cttggcccat tccacggttg ccgggcgtac caaagatcga ccaccatatc gatgcgtaaa
     2881 cctaaggtgt ccggcagctc gttacgacgg gagatatcta cccccaggct tgccatcaca
     2941 tccagttcgg ctcgatccca taaaggttct gagtcgataa gtaatccatc catatcaaaa
     3001 attgcagcaa gaatctgacg cggggttgac attacaacct ctcctttaga gcattatgtg
     3061 taaggtatta agccgtgctt atgattaagc gcctatcata tcgcctttaa agaatgggcg
     3121 aaaacagata tctacaggta aactaagtga aacgacgcgt cttaatcaag gagaactcat
     3181 gacgtatcaa caagctggac gcattgctgt tttgaaaagg attttgggtt gggtgatttt
     3241 tatacctgca ttgatctcta cattgatttc tttgctgaaa tttatgaata cccggcagga
     3301 aaaccaggaa ggcattaatg cggtcatgct cgactttgct catgtcatga tcgatatgat
     3361 gcaggcgaat acgccttttt tgaatctgtt ctggtataac tccccgaccc ctaattttaa
     3421 tggtggtgtg aacgttatgt tctgggtgat tttcatcctg atttttgtcg gactggcgtt
     3481 gcaggattcc ggtgcccgga tgagtcgcca ggcgcgcttc ttgcgggaag gtgttgaaga
     3541 tcaactcatt ctggaaaaag ccaaagggga agaggggcta acgcgtgaac aaattgagtc
     3601 ccgtattgtt gttccgcacc atactatttt cctgcagttt ttctcactat atatcctgcc
     3661 ggttatttgt attgctgcag gttatgtgtt cttttctctg cttgggttta tttaatcaaa
     3721 aggccgcacg gagcggccat tatcctcaac ccatatcatg ctgctaataa tctatccaat
     3781 gactgctgag caataaccaa atgctgaccg ccaaataatc ttgcacgatt taacagcgtg
     3841 tagagttggt aaaccggttg acgttcaagg aaatcggcag gtagcgggga tactgactga
     3901 tagccgtcat agatttgtgg cggctgttca gtatgcagcg gtaacatcgc taggtcgcac
     3961 tctcggtcac cccagtcgca ggcagggtcg aaaatgtacg ggccatccgg acccagcgca
     4021 cagttgccgg accataaatc gccgtgcaac agagaaggct gcggttgatg cgaggccaga
     4081 cgctgctgaa tatgctcgac gatggcgtcg atattgccga aagcgatccc tttctctgct
     4141 gccagttcca actgccagcc aatccgttgt tcagcaaaaa acgttgacca gcgacgttgc
     4201 caggtgttgg gctgtggagt tgtggagagc gagttatcaa aatcgaggcc aaattgtggt
     4261 tggtcactcc attgatgtaa acgcgcaatt tgctgaccaa gaataaatgc gctatgcgca
     4321 tccagcggac gaggtgggag ataatccatc accagaaaac tgtagtcacg gtcagcgcct
     4381 actgcccaaa ccttaggcac ggtgacggtt ttactacgcg acagtaactc cagttggtcg
     4441 gcttcggcgg taaaaccggg aagcagttcc ctttcatcac atttgacgaa aaagtcatgt
     4501 cctgcatagc gcaaatgcca tgcggcatgg acttctccgc caggcagttc attacgcagt
     4561 tcgatttcgc cttcacctaa ctgctcgctc aaaagacgac tgattgcctg ccacatgatg
     4621 tctctcccat gttgtctgcc agatcataag gttagcgcaa gaatgcggtg taaaaacacg
     4681 agctaacgca caccacagac tgattattgc ccaaaaacca tttattttct gttgctattc
     4741 cattcctccc aggtcgtgat ggtgatttca ggatctttgc cggttgcact ttgggtaagc
     4801 cccgaaacca gttctctaat ttcttcttca ctttgggtac tgataagtcc aaaagtgtta
     4861 gtccccagct catggatatt tccctcatca tccgtcatgg tgagcaaaaa accgtcgcga
     4921 gtcaggtaat tattcaactc gttaatttca gtcaatgtat cctcatgcaa catgacggtt
     4981 atgacataat ggacaagatc gccactggcc atagttcacc cctttgttat cacatgactt
     5041 ttgagcatag tcggagaaac gcgttgccga caggttggtg gtgattcccc caatgctggg
     5101 ggaatgtttt tgttagcggg aaaggtaagc gtaaattttt tgcgtatcgt catgggagca
     5161 cagacgtgtt ccctgattga gtgtggctgc actccccgca gctacgccaa aacggaccat
     5221 ctcttcaaga gaggcatttt ctgccagttt cagtgtcatc gcgccgacca tgctgtcacc
     5281 agcgccaacg gtactctggc ttttcaccgg tggtggcacc acctgaatgc agttttcact
     5341 atcgacaccc agcgctcctt gtggaccaag ggaaacgaca acccgtttgg ccttgccgct
     5401 attaacgatt tcctgcgcgg ctttgcggac atcgtctggc tgggtgagtt cgcgattcac
     5461 cagcgcactg agttcttttt ggttaggctt aaccaactcg atgttaccaa tcgccagtgc
     5521 tgcacttaac gcttcgccag aactgtcgat gatgcagcgg atcccttggt tttgcgcagc
     5581 ggaaatcagt tgggttaatt tttccagctt cacacctggc ggcaggcttc cgcttatgac
     5641 caggatggcc ccggattcaa tttccagaac ttgctcttca agctggcgaa actcatcttc
     5701 atttaatgcc gcgcctggca taacaaaacg atactgttca ccgcttgctt ccacatgtac
     5761 gtgtaaattc tgccgggtcc agtctttggc ttctacagta gcgacgggga cattttcatc
     5821 cgccaacagt gaaaccaggt gttcgccggt cgcgccaccc gccgggaaga tcgctgtggc
     5881 actgcctcca agatgggcaa tggcgcgggc gacgttgatg ccgccgcccc cgggttcgaa
     5941 caccggtgcg gtacagcgca gttttccttc gggataaatt tgcggggtaa ttgttgcgct
     6001 atcgagagag ggcgcaagtg tcaacgtata gatacgtacc atcatttcct cctataggct
     6061 gatttcagtc tggcaccgaa tcagcggaaa gtgaagaa
//
LOCUS       DAZOYF010000201         6052 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZOYF010000201 DAZOYF010000000
VERSION     DAZOYF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.203"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            430..1338
                     /locus_tag="ACHUOF_003773"
     CDS             430..1338
                     /locus_tag="ACHUOF_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFR0612772.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="ACHUOF_003774"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="ACHUOF_003774"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0612773.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="ACHUOF_003775"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="ACHUOF_003775"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR0612774.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="ACHUOF_003776"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="ACHUOF_003776"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR0612775.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="ACHUOF_003777"
     CDS             complement(5673..>6052)
                     /locus_tag="ACHUOF_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612776.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1576 c         1561 g         1424 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccggatggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgccaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 tcgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DAZOYF010000202         5986 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZOYF010000202 DAZOYF010000000
VERSION     DAZOYF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.204"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..251)
                     /locus_tag="ACHUOF_003778"
     CDS             complement(<1..251)
                     /locus_tag="ACHUOF_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612777.1"
                     /translation="MNAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWF"
     gene            669..1667
                     /locus_tag="ACHUOF_003779"
                     /pseudo
     CDS             669..1667
                     /locus_tag="ACHUOF_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(1754..2230)
                     /locus_tag="ACHUOF_003780"
     CDS             complement(1754..2230)
                     /locus_tag="ACHUOF_003780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612778.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(2223..2987)
                     /locus_tag="ACHUOF_003781"
     CDS             complement(2223..2987)
                     /locus_tag="ACHUOF_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0612779.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            3233..3994
                     /locus_tag="ACHUOF_003782"
                     /pseudo
     CDS             3233..3994
                     /locus_tag="ACHUOF_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(4101..4613)
                     /locus_tag="ACHUOF_003783"
     CDS             complement(4101..4613)
                     /locus_tag="ACHUOF_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612780.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(4752..5900)
                     /locus_tag="ACHUOF_003784"
     CDS             complement(4752..5900)
                     /locus_tag="ACHUOF_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0612781.1"
                     /translation="MSITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQP
                     LGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDR
                     KLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAH
                     QSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDG
                     TLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTS
                     QLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDG
                     IAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT         1771 a         1270 c         1085 g         1860 t
ORIGIN      
        1 taaaaccata ttttctccat ctgaagacta tgttcattta ccctccagtg accatgttgc
       61 aggaaataga tgatatgcat gccaaaatcg ttatctattt gcgaatcatt atccagctta
      121 tcaatttcaa ctatctttga acgatgtgtt ttatcaacct tgctatggtg ttgatgatta
      181 ttaactttat ctgaatgctt atctttatgg tttgaaggga tgtacttaaa tatattgtca
      241 attgcgttca taaataaccc gcgatatttt tgacctgtta gtaaaaaata aatgatacat
      301 gtctgaaaga catatttacg acgatataaa acaataatac ttatatgttc gctgatttta
      361 agcaccgaga ctatccggtc ttttactaaa taatggaaaa caatccatcc ctggaagaca
      421 attgtaaaaa aaaacaacag aataagaatt aatcttagga taaatattta tttatcatgg
      481 ccttttgacc gctaaaaaat ggcactcggc acagtaaacc ccgctatagt tatgctatgc
      541 atttgtcttt aatgatatct cgtcgttata ttaacaggct gaaattatca ttaatgcaca
      601 tttcaatatt agcagggata cctgaggagt ttaaagtgaa catatatatc gggtagcttt
      661 tcaaattaat ccctttgatt ataggcttaa tttgcatcgc gttaggtggc tttgtgctgg
      721 aaagttcagg gcaacgtcac gggtcatgtg ctgatttctc tggcggccat atgcctggca
      781 ttattcacta ccgcatttat tatcatttcg caactcacgc gcggcgttaa tacgttttac
      841 aatacattgt tccccattat tggctatgcg gggtcaatta tcaccatgat atggggttgg
      901 gcactgttag caggtaatga tgtgatggca gacgagtttg ttgccggcca tgttattttt
      961 ggcgttggta tgattgccgc atgtgtatcg acggtggcag cgtcgtccgg tcactttctg
     1021 ctcattccca aaaatgcagc ggggagcaag agcgacggaa caccggtaca ggcttattct
     1081 tcattaatcg gtaattgcct cattgccgtt cccgttttac tcaccctgct cggtttcatt
     1141 tggtctatta cgctgttaca tagtgccgac ataactccgc attatgtcgc cttgggttaa
     1201 ccgcaatctg tgcctgtcta attggcctag tggccacaat cgtccatcaa acacgtaata
     1261 cgttctcatc caaagagcac tggctgtggt gttattgggt tatttttctc ggctcagtca
     1321 cggtgctgca ggggatatac gtcttagtca gttccgatgc aagcgcccga ctggctcccg
     1381 gcattattct catttgcctc ggaatgatct gttacagcat attctcaaaa gtctggctac
     1441 tggcactggt ctggagacgt acctgttcgt tagccaacag aataccgatg ataccggtat
     1501 ttacctgtct gttttgcttg tttttgtcat cattcctcgc ggaaatcgca caaactgagc
     1561 tggactactt tattccatct cgggttctgg ttggattagg cgcagtatgt tttacattgt
     1621 tctctattgt ttcaatatta gaggccggtt ctgcgaaaaa aaattaaaat ttacataatc
     1681 ccagcgttat tcatatctta acgctggttt tttacgaact tagtgaaata atgtagaata
     1741 taaaattata attttatatt ggagcacatg tgttaatgat caaccccggg atactaaaaa
     1801 tatagacatt attaaacttg cttagcctcg cattgccttt acctgttaaa gatgcatcga
     1861 gaaaattgag tctgaagttc tcatccttta tctgattttc cagtcccgaa actttggcct
     1921 tagcattacc tgagatccgt ccgttactat ctattttgta ggtaaaaaaa tacgaatggg
     1981 aaaatttcct cggtgtatct tcatcagtta agccaacaat cgtaaaacta ccgctaccat
     2041 ctttttttaa aatgactgaa aggttagcag agtagtataa agaatgatct gttttagcat
     2101 ttcttgttaa cgtcgacgta cattcaagag acgttgtgct accatcacga gccttccaga
     2161 gagcaaaaaa acacacctgt agcaatgtga aaacaagtat taatataaaa tacattttct
     2221 tattagtcat ataatagcac ctctgaacaa attgcataag tttcgtcatt agaggatttt
     2281 atacatcttg ataaataccg ctgcccttcc ccaagcaaat aatgcatact atttacatca
     2341 tatataaaat ttgcacctgg cacacagggt aaatgctttt tcgaaatttc agcggcctct
     2401 atttgagatt tagcgatgcc aatctcgtta agattctctg aaagatataa atcacaggtg
     2461 tcaatcttac ctgatttaac caatggataa tccgttatac tgtaggtatc tttaaaagcc
     2521 acggcaaaca tttcaataat aacaattact accacaagcg aaatcaaaat gcgaaaaaga
     2581 ggaatactcc ttttgcgctc tactttttca acaacatctt cttgtaaatg agaatcatca
     2641 taagattgtt tacttattgt tatgttttca ctgatgtaaa agcctacttt gggtatcgta
     2701 agaatcacat tttcttcaac accaatattt ttaaaacttt tgcgtaacag ggatatatat
     2761 tggttcaatg tgttgtttga cagatcaaat gaaaatctct ccaaaacgct atcaatgatt
     2821 gagttcctgt caaccgctcc accattactt tccagtaaca ctgccaaaat acaccccgcc
     2881 gtagctggaa aatttatctc atcctgggta tttacattcg agagcgagca tttgttcgga
     2941 tcaaaaataa tcgcgtcatt gataatgaaa aaacatctcc gctccatgca ggcaacaaca
     3001 caaaataaga ttttgtcatt ttagcctatt cctggtggaa ctgacacaag aaactaaaat
     3061 acaaatataa ccctgaaaaa gtcatataaa acttatatcc acagaataaa aaatcaaaca
     3121 tgtcagtttt atcaatataa agtatcgcaa ggcgaaaagt gatgcaagaa tactcctaag
     3181 taaatgaaag cgcaccacat gaaattatca acacatgtaa tttcatcatt aggtgctatc
     3241 gaaaataact caggagctac ttaagatgaa taatgtctta ctcatgagac ccgatgactc
     3301 agaatttatg atacctgatg actggtcagg gctaaacgca aaaggcatta catacgaaca
     3361 agataataga gtgacagagt cttttctgcc ttttaaatct ttttattgtt caccggtgtg
     3421 cacgacagtt gaactcatct cataaaataa ttgttttgct atgggtatcc gctaccttct
     3481 ggaaaagaaa agagacttaa ggctaaatca ctttaaagcc ttctctcata aatgcgagac
     3541 aaaacctgac gtgttaatta tgaccgggtg caccgtcgct gatacttata gtatattctc
     3601 acgcattgtt gaagaaaata taatgaaaac gatgacacct atcgtcatca ttactgggca
     3661 tcactcagaa gatctttgtt ctctggcatc gaaactggga ggagttaaaa taatatcacc
     3721 gacagaaaag attgcaaaaa tagaaaacgc tatcaatgat gcttttaaca gcaagcctaa
     3781 aaaaataaga tttttaacag atatactctc tcctcaacaa attgagacca tgaaaatggt
     3841 tgcgcagggt agatccgtta atgagatttc agccgcaatg aatattagca tcaagaccgt
     3901 gtatgcacat aaagttcgtg ctatcaataa gctgggaatt aagaaaaaag tttatgaagc
     3961 gcttttttat aaagcgctaa ttcagttcaa ctgatgtaaa aatcaccctc gcttaataaa
     4021 taatatattc tatgtctttt tttataagtt aaaaaaggcg ggatatcaaa tcccgccatg
     4081 aaattaacta atcatctgca ttattctgcg tagatataag cctgggtacg gatggtgtaa
     4141 gagtctggct caacaacctg ttctttcata tgggaaacga cgcgataggc accacctgtt
     4201 ccagtatttt tcagcatcat ataacgctta ccgccaatct cttcggaggc agctgaagga
     4261 ggatcctgct caagcgttgt cagggaaact ttcagtttat ggtccgggtt attgtcacca
     4321 gaaattactg catccgctga agtactgctc tccgcataaa tatcatccgg gaaagttaca
     4381 gcatagtcgt ggctctgccc atcagatgcg ccaacaatac ctgttgccaa aggcgtccat
     4441 gcagttaacg taccgctata tacccctttc actggttgaa tggtattatt aacaacgata
     4501 ggcttggtca aagtaagatt agctgacgct tcagtaccct ggcttactaa agttcccgcc
     4561 tgagaagcaa aagctgtccc cgccagcatg attgctaaca atgttttctt cattatattt
     4621 tctccatgat tttcatttac actgcaaaat gcagtttctt tctttgcatt tacgtgctat
     4681 cgcgaaaatg cttccaccaa ataatggaaa catcttcaca aaaataaatt atgagtttct
     4741 taatttagaa actataaaca tgtcgccgct atctggtata accctgatgc acttttcttc
     4801 gcaggaagtt ggtattttgc gatacaggat tgatctttag aataacccca tttcacatta
     4861 aactcgcctt ccgcactcaa gccagaaaca taagcaatac catcttcacc tacaatgccg
     4921 gccatatttt cagtgtcttt gctataacta atcacagcgc cgaaggggat gggtttaccc
     4981 tctttctgtt ttaaggttac taatccttta tccccgctct tcactgccaa tgtcgcaggc
     5041 acaacggctc cagctgtcgg tacaaccaat tgactagttt gtggtaagtc gtaattctta
     5101 cctatcgttt tttcatccag aataacatcg tctcttcggt atggctgcac tggcagtatt
     5161 gcatacccac gaaaatcagt actcagtccc ggccagccat taacgttaac ccccgttgct
     5221 ccgggtgcct ctataagggc taatgtccca tccacatttt gaccaaatgt aagtccatcc
     5281 cttgtaataa gtatcccacc gcttaaattt gcacctagct gtttcatgtt ttcgctggag
     5341 ctgtaatggc tactaaactg accatatgat ccatttttgc caacttgcac aatgttcatc
     5401 gtgctcttcg gttgtgactg atgagcgtta gttatatttg tcgctacatc ccaccataca
     5461 ccaccactat tctgacctga taatgaaacc tgatagttat taccctgctc tcccgaacga
     5521 ctcatctgtg aagacgcata aatttgattt tcgtcaccca gcgataatgg cactgaaata
     5581 cccacgctta atttccgatc tactttccat ttatgcgatg tcagcaagtc gttttttgcc
     5641 cagttaatgg agaatgttat gcctctccat accgtactat aactcaggct ggtagaccgc
     5701 gtgtttcctg tgccccggga agaattatac ctcgtggtat tgaagttcaa attcccgata
     5761 ttaccaaggg gctgggataa ggatatcgta gagccatcgt gactattctt atctcttttt
     5821 ctgttccgtt catcctgtgc acttactgct tcagtcagtg tggcataacc tgatgcataa
     5881 cggttcgtgg ttatagacaa tagactggcc ccctgaatct ccagacaacc agtatcactt
     5941 atttaagtga tagtcttaat actagttttt agactagtca ttggag
//
LOCUS       DAZOYF010000203         5983 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZOYF010000203 DAZOYF010000000
VERSION     DAZOYF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.205"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(117..435)
                     /locus_tag="ACHUOF_003785"
                     /pseudo
     CDS             complement(117..435)
                     /locus_tag="ACHUOF_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="ACHUOF_003786"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="ACHUOF_003786"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFR0612782.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="ACHUOF_003787"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="ACHUOF_003787"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR0612783.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..>5983
                     /locus_tag="ACHUOF_003788"
     CDS             5797..>5983
                     /locus_tag="ACHUOF_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0612784.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1840 a         1355 c         1018 g         1770 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg agg
//
LOCUS       DAZOYF010000204         5924 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZOYF010000204 DAZOYF010000000
VERSION     DAZOYF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.206"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            231..1055
                     /gene="gadX"
                     /locus_tag="ACHUOF_003789"
     CDS             231..1055
                     /gene="gadX"
                     /locus_tag="ACHUOF_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR0612785.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1423..2151
                     /gene="gadW"
                     /locus_tag="ACHUOF_003790"
     CDS             1423..2151
                     /gene="gadW"
                     /locus_tag="ACHUOF_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR0612786.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2514..5627)
                     /gene="mdtF"
                     /locus_tag="ACHUOF_003791"
                     /pseudo
     CDS             complement(2514..5627)
                     /gene="mdtF"
                     /locus_tag="ACHUOF_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5652..>5924)
                     /locus_tag="ACHUOF_003792"
     CDS             complement(5652..>5924)
                     /locus_tag="ACHUOF_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0612787.1"
                     /translation="NFARFILLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAI
                     GDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
BASE COUNT         1542 a         1411 c         1414 g         1557 t
ORIGIN      
        1 tagcgattac ctttaatgtc acgaaaaggg attatttgct tgctattaat ttcactgcgt
       61 tcttaaacac gtttttgtat tataaatcca gtcatcctgc ccgtagaaca tcacacatta
      121 tcatcctgtt ctcccgcttc gtttaaattt atttatcaat caatttgact taagagggcg
      181 gcgtgctaca ttaattaaca gtaatatgtt tatgtaatat taagtcaact atgcaatcat
      241 tacatgggaa ttgtctaatt gcgtacgcaa gacataaata tattctcacc atggttaatg
      301 gtgaatatcg ctattttaat ggcggtgacc tggtttttgc ggatgcaagc caaattcgag
      361 tagataagtg tgttgaaaat tttgtattag tatcaaggga tacgctttca ttatttctgc
      421 cgatgctcaa ggaggaggca ttaaatcttc atgcacataa aaaaatttct tcattactcg
      481 ttcatcactg tagcagagat attcctgttt ttcaggaagt tgcgcaacta tcgcagaata
      541 agaatcttcg ctatgcagaa atgctacgta aaagagcatt aatctttgcg ttgttatctg
      601 tttttcttga ggatgagcac tttataccgc tgcttttgaa cgttttgcaa ccgaacatgc
      661 gaacacgagt ttgtacggtt atcaataata atatcgccca tgagtggaca ctagcccgaa
      721 tcgccagcga gctgttgatg agtccaagcc tgttaaagaa aaaattgcgc gaagaagaga
      781 catcatattc acagttgctt actgagtgta gaatgcaacg tgctttgcaa cttattgtta
      841 tacatggctt ttcaattaag cgagtcgcag tgtcctgtgg atatcacagc gtgtcgtatt
      901 tcatttacgt ctttcgaaat tattatggga tgacgcccac agagtatcag gagcgatcgg
      961 cgcagggatt gccgaaccgt gactcggcgg caagtattgt tgcgcaaggg aatttttacg
     1021 gcactaaccg ttctgcggaa ggaataagat tatagagttt tactcagaca taaaaaaaac
     1081 ccggcatagg ggaccgggaa gaggatagtc tgccgtctcc agactaataa accgttataa
     1141 cactccctgt tggcacggga aactttgtgc tctcagtaag ttaaatataa cttttacagg
     1201 aaataagatc agccattttt ttataaacat aagctatacg ctgtgcgaaa atatattcgt
     1261 gctgcattta cttattatca attaactgtt atgcaaaact actttgtgga taaattttgg
     1321 tcctaccaaa tctggcagtt tttgcgctaa gaaacagtct gtcatcattt cattagtata
     1381 ctgaaattga aataatcgca gtatgaaata taagggataa tcatgactca tgtctgctcg
     1441 gtgatcctca ttcgtcgttc attcgatatt tatcatgaac agcaaaaaat atcgctgcat
     1501 aacgagagta tcctgctgct ggagaaaaat ttggcagacg attttgcgtt ttgttcaccg
     1561 gatacgcgac gactggatat cgatgagctg acagtttgcc attacttaca aaatattcgt
     1621 cagctaccac gcaatttagg gttacatagc aaagaccgtc tgttaattaa ccagtcaccc
     1681 cccatgccgc tggtgacggc gatttttgat agcttcaatg aatccggggt aaattcaccg
     1741 atactgagca atatgctcta cctttcctgt ttatcgatgt tttctcataa gaaagaactg
     1801 atccccttac ttttcaatag catcagcact gtttcaggaa aagttgaacg ccttattagc
     1861 tttgatatcg ccaaacgttg gtatctgcgc gatatcgcgg aaagaatgta taccagcgag
     1921 agtctcatca aaaaaaagtt gcaggatgaa aatacctgtt tcagtaaaat attactcgct
     1981 tccaggatgt cgatggccag acgattactc gagttacgtc aaattcctct gcatactatt
     2041 gcggaaaaat gtggctatag cagtacgtcg tactttataa acacatttcg acaatattat
     2101 ggtgtaacgc cacatcagtt tgcgcaacat tcgccaggta ccttttcctg acatattttg
     2161 catttgaata ttggtcagga tctcacacct gcttcatgtg aaactcttcc ctgatgattt
     2221 ctgccgggct accggctagt tctctttcgc agtttgccag gcggtatagg tatttttagg
     2281 aggaaaagac tcggcatgtt tgggattatt aagctgacaa ttcatacgat tacgggaatg
     2341 tgggtcagta ttgtgttgtt caaattgatg acgaatggct ggagtggttt ctactttcag
     2401 tgctgtgtac taagcctggt attcctgacg gttagctggt tgttgagtgg tgaatggtta
     2461 gcaggaaaga gtaaggctga accttcacgt tcaaccttac tctcatttac acgttacgct
     2521 tttttaaagc gggcaaagag atgttccact acaacgaaaa agaccggaac gaagtaaatt
     2581 gccagcactg ttgcggcaaa catcccgccc atcacgccgg tacctaccgc gttttgcgcg
     2641 ccagaaccgg caccatgact gataaccagc ggcagcacgc cgagaataaa ggccagagag
     2701 gtcatcagga ttgggcgtaa acgcatccgc gccgcttcga tgattgcctc tatcggcgtt
     2761 ttcccttctt tctgcatcat ctcaacggca aattcgacaa tcaggatggc gttcttggcg
     2821 gaaagcccga tggtggtcag caaaccaacc tggaagtaga cgtcattact taagccgcgc
     2881 agatcggtgg ccagtaatgc gccaacgacg cctaacggaa caaccaacat caccgagaac
     2941 ggaattgacc agctctcata gagtgcggcg agggcgagga acaccacgac cagtgaaatg
     3001 gcatacagtg caggagcctg gtttgaggat aacgcttcct gatacgacag tccggtccat
     3061 gagtagccga cgcctgccgg aagtttagcg accaggtctg ccataaattt catagcgtca
     3121 ccggtacttt tcccggccgc cgcttcacct aaaatctcca ttgacgggat gccgttgtag
     3181 cgttccaatc gcggtgaacc ataggtccat tcggtagacg agtaggcaga aagcggtgcc
     3241 atcgtgccag aggcgttgcg tacataccat tggttgatgt tatccggcaa catacggaac
     3301 ggcgtgcctg cctggacata cacttttttc acccgcccct ggttgaggaa gtcgttcacg
     3361 tagctgctgc cgaaggcggt ggaaattgtc tggttgatat cagacagcgc cacacccatt
     3421 gcctcagctt tcgcggcgtt gacgttcact ttgaacatcg gcgtatcttc caggccgttc
     3481 gggcgtaccc cggtgacctg gtcaggcgat tgcgctgcca gtgataacag ctcgtttcgt
     3541 gcctaggtta gtttttcgtg ccccaggtta ccgttgtcca gcagttccat atcaaaacct
     3601 gacgcggtac ccagttcagc caccgcgggt aagttgaacg ggaagacgac ggctttattg
     3661 atactgctta acgcaatcat tgcccgctga atgatcgcgg taaccgagtt ttcctcaccg
     3721 acacgttcag accacggctt gagactgata aacgccaggc cgttgttttg cccctggccg
     3781 ctgaagccaa agccgccaac ggtaaacacc gactgaacat tatctttctc ttttgtaaga
     3841 taataatccg tcacctgttg cagtactttc gtggtgttaa ccatggtggc accggaaggt
     3901 aactgcgcgg tggtcataaa taccccctga tcctcttctg gtaagaaaga ggtcggtgta
     3961 cgcaggaaca gcaccgccat cccggcgcaa atcagcaggt agaccaccat gtagcgaccg
     4021 gtacaacgca acagcgagcg ggtgctatcg gtatagtgct gagttgattt ttcaaacagc
     4081 gtgttgaagc gtgcgaacag ggcgttaggt ttgtgaccgc cttccggcgt ggctttcaga
     4141 atggtggcgc acagggcagg ggtcaggctc attgccacaa atactgaaag ccgcatggag
     4201 gagatcagcg tgatggagaa ctggcggtag atctccccgg ttgcaccgct cataaaggcc
     4261 atcggcataa acactgcaga aagaacaacg gcaatcccga ccagcgcacg ttggatctgc
     4321 cccatcgatt tatgcgtcgc ttccttcggc ggtagcttat cttccgcaat gacacgctcg
     4381 acgttctcca ccaccacgat ggcgtcatcc accagtaacc ctatcgccag caccatcccg
     4441 aacatagtca acgtgttgat ggtgaaaccg accgccgaca agatcgcaaa cgtcccgaga
     4501 ataaccaccg gtacggcaat tgtcgggatg atggtggcac ggaaattctg caaaaacaga
     4561 taaatgacca ggaagacgag gatgatagcc tcaaccagtg ttttgaaaac ttcctgaata
     4621 gaaatttcga taaacggcgt ggtgtcgtaa ggataaaccg tcttcagact tgcggggaaa
     4681 taggctgata agcggttcag ctcctctttg actgcccgcg aggtatccag ggcgtttgct
     4741 ccggcagcca gtttgatggc gatcccggca acaggtttgc cgttatagcg cgccacggtg
     4801 gaataatctt ccgccccaag ttcgacgcgg gcgacatcac gcagcagcac ttgcgaacca
     4861 tcttgctgaa ctttcaacag gattttgcca aattcttccg gcgtttgcag acgcgtctgc
     4921 acaatgatcg aggcgtttag ttgctggtct gccgcctgtg gcatgccacc cagttgacca
     4981 ccagaaatct ggttgttttg caccttaatc tgggaaataa catcggaagg taccaggtta
     5041 tatttattga gtttttgcgg gtccagccag atacgcatgg cgtactcgga accaaagagc
     5101 tgtacgctac caacgcccgc ggtacggctt agcgggtctt tgatattaga cgctacatag
     5161 tccgcgatat cgtactggtt gaggctgccg ttatcagaaa taaacgccgc taccatcagg
     5221 atattactgc tcgacttatc gacgctaatc ccctgttgct gcaccgcttc aggtaatgaa
     5281 ggcatagcga gttgcagttt attttgcacc tgaacctgtg cgatatcagg agatgcccca
     5341 gtctcgaagg tcagagtgat agaggcatta cccgccgcat cactggttga agacatgtac
     5401 atcaggccat caagcccatt catattttgc tcaatcacct gagtgaccga gtcttctacc
     5461 gtttgcgcat cggcacccgg ataggtagcg ctgacggtaa tggtcggtgg cgcaatctgc
     5521 ggatactgcg caaccggtaa gttcatgatc gccagaccac ctgcaagcat cataataatg
     5581 gcaagtaccc aggcaaaaac cgggcgatca ataaaatagt tagccatgaa agtcccctta
     5641 agcctgcaac gttattgttt cgattcggtg ctggcgtttt cctggctgga ggaaattgct
     5701 cgtgctttga tacccggacg aatgcgttgc aaaccggaaa cgatcacccg atcgccagcc
     5761 tgcaagccag aggtgacaac ccactggtcg ccgatggctt tgctggcttc aatttcgcgt
     5821 agctgcacga catcgtcttt atccagaatg agcgccgttg ctttaccctg ggcgttgtgg
     5881 gtgacgcctt cctgcggcac cagtaatata aagcgcgcaa aatt
//
LOCUS       DAZOYF010000205         5899 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZOYF010000205 DAZOYF010000000
VERSION     DAZOYF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.207"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..411
                     /locus_tag="ACHUOF_003793"
                     /pseudo
     CDS             1..411
                     /locus_tag="ACHUOF_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="ACHUOF_003794"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="ACHUOF_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACHUOF_003795"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACHUOF_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HFR0612788.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
     gene            5722..>5899
                     /locus_tag="ACHUOF_003796"
     CDS             5722..>5899
                     /locus_tag="ACHUOF_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612789.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQD"
BASE COUNT         1445 a         1488 c         1409 g         1557 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tagggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc gagaaccacg gcagcctgac ggctgcaaat tgaaaactgc
     5641 ccctgttcgt cctggtgttg ccgcacgctc cgcagaatat gtatgctctg aggacctgtt
     5701 ttatttagga ctcatcctct tatgttgacc cgcaaaagta tagataccgt tctgctctct
     5761 gttggtgctg agaagctctc acaacgtgaa tgggactgga tgaagatgct caaacccatg
     5821 gacccaccac ctgcgatggt cgcggcctcc atcctggagc gccgtggcga cacagcagcc
     5881 cttacccggc tacaggata
//
LOCUS       DAZOYF010000206         5891 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZOYF010000206 DAZOYF010000000
VERSION     DAZOYF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.208"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            194..466
                     /locus_tag="ACHUOF_003797"
     CDS             194..466
                     /locus_tag="ACHUOF_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR0612790.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(432..839)
                     /gene="nudG"
                     /locus_tag="ACHUOF_003798"
     CDS             complement(432..839)
                     /gene="nudG"
                     /locus_tag="ACHUOF_003798"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR0612791.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            926..1564
                     /locus_tag="ACHUOF_003799"
     CDS             926..1564
                     /locus_tag="ACHUOF_003799"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0612792.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1573..2880)
                     /gene="ynjE"
                     /locus_tag="ACHUOF_003800"
                     /pseudo
     CDS             complement(1573..2880)
                     /gene="ynjE"
                     /locus_tag="ACHUOF_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(2947..3600)
                     /locus_tag="ACHUOF_003801"
     CDS             complement(2947..3600)
                     /locus_tag="ACHUOF_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0612793.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3600..5071)
                     /locus_tag="ACHUOF_003802"
                     /pseudo
     CDS             complement(3600..5071)
                     /locus_tag="ACHUOF_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5044..5891)
                     /locus_tag="ACHUOF_003803"
                     /pseudo
     CDS             complement(5044..5891)
                     /locus_tag="ACHUOF_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1378 a         1593 c         1562 g         1358 t
ORIGIN      
        1 gtttatgtca tgtgcttttg cagttttcga ctataccatc aagaatgtga cggtaagcaa
       61 gaaaaaaatg acccaggaaa gcaaattaat aacgagagta atctcataac caaatgcgat
      121 aactcagacg ctaaagtttt gccacccggc taccgttatc agtagcagag acaataacgt
      181 taaggagttc cctgtgagtc gagcattgtt cgccgtagta ttagcatttc cattaattgc
      241 actggctaac ccgcattatc gtccggacgt ggaggtaaac gtaccgccag aagtgttcag
      301 ttcaggcggg cagtcagcgc agccctgtac tcagtgctgc gtttatcagg atcaaaatta
      361 ttctgaaggg gcggtaatta aagccgaagg aatactgctg caatgccagc gtgatgacaa
      421 aacccttagc actaatccgc tgctctggcg gcgcgtaaag ccataaacgc ctctaataat
      481 ggaatgtcag caggggccag cggatattgc agcgcctctt caggtgagca ccagaccagc
      541 gcctgatgtt catgtgcctg taacgtcccg tggaagtcgg gtacgtgcca ggcatgaaga
      601 tggataatcc ggcccgaaac ttctcgctga tggctggcaa catactcacc cacagttgct
      661 tcgatgtcca attcttcgtt taactcacgc accagcgcct gctgctggct ttcatccagc
      721 tcgactttac ctccggcaaa ctcccataat cccgcctgat cgctctgggc ggggcgttgc
      781 gcgagtaaaa ttttgccatc acgttcaatg atggcggcaa caacttcaat cattttcata
      841 aattacacac gggcagataa gcgaaaaatg ccataatatc cgcactgttg ctcaatcaga
      901 aaggctttat atttaaggag tgcctgtgct agaccgccat cttcatcccc ggattaaacc
      961 gttgttgcat cagtgcgtgc gggttcttga taaaccgggc attacgccag atggtttaac
     1021 attagtggga tttgccatcg gcgtgctggc tctgccgttt ctggcgctgg gctggtatct
     1081 ggcggcgcta gtcgtcattt tgttgaacag gctgcttgat ggtctggacg gtgcgctggc
     1141 gcggcgcaga gggcttaccg acgcgggcgg ctttcttgat atttctctcg attttctctt
     1201 ttacgcgctg gtgccgtttg gctttattct tgctgcaccg gagcaaaatg cgctggcagg
     1261 cggctggttg ttgtttgcgt ttatcggcac gggcagcagt tttctcgcct ttgctgcact
     1321 ggcagcaaaa catcagattg ataaccccgg ttatgcacat aagtcgtttt actatctggg
     1381 gggattaacc gaaggcacag aaacgatctt actgtttgtg ctgggatgtt tattccctgc
     1441 atggtttgcg tggtttgcgt ggtttgcgtg gtttgcgtgg atcttcggtg cgttgtgctg
     1501 gatgacgacc tttacgcggg tgtggagtgg gtatctgacg ctgaagtcgc tccagcgtca
     1561 gtaattgcaa tgttatttgc tactgcccgg gccgcgttca ccggttgcaa ccggattttt
     1621 tggatcgctg ctccattcgt accagccgcc gtcatacacg gaaacattct tccaacccat
     1681 ggcgcgtgcg tacataaagg tttcggacgc gcgccagccg gtgccgcagt agaatgaaac
     1741 ttgctgatct ggtttgatat tccatgcttt ccacatagcg gtaatatcat cggcgctacg
     1801 catggtgcca tccgggttat agaaatcttc catatgcgtc gagtcgctac cagcgtgtcc
     1861 ccaacgtgct ccggctattt caccttttgg tttaatatag ctgtaaccgc tggtcgtacc
     1921 gataaattct ggccacgaac gaatgctcac cagcgatgca tcctggcgat gcagcagtcc
     1981 acgggcctgt tccatatcga gcatcaactg cggttgtgcc gggatcttca cgccgaaatc
     2041 cggttccgct ttcactttcg gtggcgttcc tcgctcaaca ggcagtcccg cgtcggacca
     2101 ggtttgccag ccgccatcca gcagacgcac atctttcacg cctgcgtaga gcataatttg
     2161 cgccacacgc gctccagcgt atacgtcacg cccgtacaga atgaccgtgg tgtcatggcg
     2221 aatgccgtgt tttgccagca tcgcttttag ttgttcatca gaaactttgt tccacagcgg
     2281 ttcgctttcc acttcgttgg tatcgatgta gtcagcgcca ggaatatggc tgataaggta
     2341 aagcttagga gcgccctagg ccgcttcaat gactttccag tcaccgacag gtttcgccgt
     2401 aacctcttta ccttgttgca ggtcatgcag ccattgcgga taaaccagct gctcaaagtg
     2461 cggcagtttt tgtagacggg aaggttcgct tagcgcgtca ctcagaatgg agatatgcgt
     2521 aaaacctgct ttttgcagtc gcgttttgac ggcgtcaaca tctttgccat taccgtacag
     2581 cgccaccggg gcatcggctt tcaggttatg ttgcttgatc cacgcgttga gctgttcggt
     2641 gctcattttg tccagccagc tagcagagag gtttaaggcg gcaggttcat gaccagaagg
     2701 gccatttaag gtttgtggcc agccgttata aaacgcgctg gggcgagtat ctatcgcttt
     2761 gccattttgt tgctgaagtt ggtcaagtgt aagaggcttc gccagttcag cggcccacga
     2821 agaagcgcaa gccagcccta aagccattgc cagcgcggtc atttgagaaa cacgtttcat
     2881 cgaaaatccc gacattgtag ttttgagtgc gccattctga actgacgcat tgagaaaaac
     2941 tttgcgttat cgcagtttgt tgtaattttc tgaccactgc gccatatcca gaacagaact
     3001 atcagcagga acatcctgga gatcgtgcgt tacctgaacg acggggatcg ccagggcgcg
     3061 aacttcgctg aacacccact ggcgaaaatt atcgcgcaga gccacatcaa gacggctgaa
     3121 tggctcatcc aggagtaacg cttttggctg ggcgagaagg gcgcgtagta gagcaacgcg
     3181 cgctcgctga ccgccagaca aagtggcagg atcctgatgg aaagctccgt caaggcctga
     3241 acgctcaagt gcatcattca cggcattacg tcgggcattc cccttaagtg tcgccggtag
     3301 cgccagcagt aaattttgcc cgacgctgaa ctggtcaaat aacagtgcat cctgaaaaag
     3361 aatgccaatc tgacgctgtg cggtgggtag catgtcaatc cgttgctcat tgagccatag
     3421 ctcacctgta caagaaaact gtccggccag tgcaccaatc atccatgaaa acagagtgga
     3481 ttttccacag ccagacggcc ccattaacgt gacgatgtca cctttatcaa ccgtaaagtt
     3541 aacgtttgtc agcaagcggc tttctggtaa acgtagcgaa acatttttca cgcagagcat
     3601 tagcggattc cttgtctgac ataacctacc cattttgcga ctaacgcggt cagggcaaaa
     3661 ataataagcg gtaacaacag ttgccataac gcctgggcgg cgagaatacc gttgctgccg
     3721 ccgctgctta atgccaccgc ctcagtggtg agcgtcggaa aacgccccgc gcccagccac
     3781 agcgtcggca tatactgcgc aatactgact gaaaatccca ccgcgaaggc aatcagcaca
     3841 gggcgcaaca tgagtgggca tttcacgtaa aagaagattt tggcccgcga ccagcccagt
     3901 gtttgcgcaa tcaaaattaa ccgtgaatca atgcgctgcc aggcgggttg caggataaac
     3961 agcatccacg gcattaccca cagcagatgc ccccagacca ccgccgtcca gcttccatcc
     4021 aggttcagcc atagcgccag cgtgtactgg cccgccacca gtggcagagc gggcaataga
     4081 atgggcaacc atagccacaa ctggcgacgc tgtgcccccc attccagcca cagcagtaac
     4141 aggagcaaag cgatgaatgt tgctaccagc cccattgtca gactgttgat gagtgcttca
     4201 ctattgatcg tcgactgatc cgcgaggatc gccagcagaa ccacacacag cacaccggtt
     4261 aagggtaaaa aactcgccag cgtagtgcct ggtaataaag gtgtggcagg cttgcgaatg
     4321 ccatctacgc ggggaatagt gcgccgccag ctgcgccaaa acgtaggcgg cgagtaacag
     4381 catcagcagc agactggcga gtgcgccttt cgtttgttga tccgcgtcgc cctgagttaa
     4441 ccactgccag ctaattactg ccagcgtcgg tggattacct ggcccaagaa taatcgccac
     4501 atccacgacc gaaagcgacc aggcaacaat cgccagcatc gccattgcca gcggctgtag
     4561 cccagcgaat ccagcacaat gacctgctgc aacagccgtt tttcgctcaa tactgccact
     4621 aagatccaca gcagaaatgc gctttctttc actgcaaggg tgaggcccag accgatccaa
     4681 atcggtccat tggcggagtg aaatacggga aatagtcata tagcagccct ccgtcagcaa
     4741 agagcagcag ggcgctggtg gcaaaagcca catggggaat ggcgagcagc cacggcagac
     4801 gggcgcacat acgctgccat ttcggcccag gccatagcgc cacaatcacc agcagggcaa
     4861 tcaacaatgc cccgaccgcc gcgatggttg tcgacaccag cgttgccagt aatgcctgcg
     4921 gtaactgtgg atcggcaaat aacgcctgcc agcgtgtcaa cgacaacgca ggcgatatca
     4981 gcgtgagagc tgccgggatc agcggtgcat aaatcaccgc caccatcgcc cacagcaaaa
     5041 agattaatgc gtaccgtaac ggcgtaacca ttcttgttcc agtgcattca cccatcctgc
     5101 gtgcggttca gccagtaccg gcggcagatc ctgcggcatt cttgattgca atatttcgcg
     5161 ctgcccgtca ggcagttttt gcggatcgag aacagaagga tcgccccaga caacgggatc
     5221 tgctttacgc agttgcgcat cgggtgagag caggaaattg gcaactacct tcgccgcagc
     5281 actggcattc gcgttggctg gaatggtgac gaaatgcacg ttgccaatca tcccctcgcg
     5341 aaagccaaaa ctgtaactgc ttgccggcaa atcgccgctg gcgatttttt gctgcgcatg
     5401 tgcggggtta aaggtcagcg acaggcgcaa tgtgccggct ttcagcagag catccatccg
     5461 cgcgggcgaa ggcgggaaat cctttccttc gcgccacaaa tacggatgca gtgcatcaag
     5521 atattgctac aagggcgcag tgacacgggc gaaagtcgca tcgtccggcg cttcttttaa
     5581 tgcggcggga tctggcgtca gcataatcag caactgttca agaaacgccg tgccggtaaa
     5641 gtccggtggg cgcggataag taaccgtgcc gggattggct ttagcaaact ccagtaaggc
     5701 ttgcggcgtt tgtggtggct gtggcgtaac atcgcggcgg gcgataaacg tcagttgtgc
     5761 gccgccccag ggcgattccg caccttgtgt cggcactgaa aaatctcccc gcaccggcag
     5821 ctgtgtgtcg acatagcgcc agttgggcag agtctccgcc cagcccgttt gtaataaatt
     5881 ggcctctttt a
//
LOCUS       DAZOYF010000207         5880 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZOYF010000207 DAZOYF010000000
VERSION     DAZOYF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.209"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..77
                     /locus_tag="ACHUOF_003804"
     CDS             <1..77
                     /locus_tag="ACHUOF_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612794.1"
                     /translation="AFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(91..282)
                     /locus_tag="ACHUOF_003805"
                     /pseudo
     CDS             complement(91..282)
                     /locus_tag="ACHUOF_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            280..604
                     /locus_tag="ACHUOF_003806"
                     /pseudo
     CDS             280..604
                     /locus_tag="ACHUOF_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(634..3546)
                     /locus_tag="ACHUOF_003807"
     CDS             complement(634..3546)
                     /locus_tag="ACHUOF_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFR0612795.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            4029..4871
                     /locus_tag="ACHUOF_003808"
     CDS             4029..4871
                     /locus_tag="ACHUOF_003808"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR0612796.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4874..>5880
                     /gene="capU"
                     /locus_tag="ACHUOF_003809"
     CDS             4874..>5880
                     /gene="capU"
                     /locus_tag="ACHUOF_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR0612797.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     AR"
BASE COUNT         1542 a         1393 c         1423 g         1522 t
ORIGIN      
        1 gggccttccc gagagtggta aaggagaggc cctggaaata ccccacagcc ccgaaaaaga
       61 gccagtcagt tgcttaactg actggcatta aggccgccgg ggacgttttt ttaccaccgg
      121 gagtttgggc ataacggtgc tttcagtaag tacgacaggt actgtggagg cacttccgta
      181 aagaagggca tcaagctcct gctccgacat acctgcaggc aaaggccatg aaagccctga
      241 tttccggaag cgagcgaaca gtccacaaac tgttgaacgt ggtgtcagcg gcggacaggg
      301 ataagccctc gtcagcagtt ccgtcagcac tgcgacagcg ttctttaccc ggtcaaaaca
      361 gcgttgcggt gcaccacgcc tgacggatga actgcgtgct cagggttacc actttaacgt
      421 aaaaaccgtg gcggcaagcc tgcgccgtca ggggctgagg gcaaaggcct cccggaagtt
      481 cagcccggtc agttaccgcg cacatggcct gcccgtgtca gaaaatctgc tggagcagga
      541 tttttacgcc agtggtccga accagaagtg ggcgggagac atcacgtact tacgtacaga
      601 tgaagttcgg ttacatcctg tcagcacgga acctcacgct ttttgagcgg cacgacggat
      661 cccccccacc cagtatccga cagacttttc actgcgttcg taaaaaccct tcttccctcc
      721 cagcatatcc actaattccg gtgacatatc ctgcagataa gtctgcaaat catgccccag
      781 atcttccggt gactgcaact cataaaccgc gcgccacaca tcacccttcc ttattttaag
      841 ccccgggtta agaatatact gtccgtggtt cagcctcaga aaaagctttt ttccataagg
      901 atttttgctg ttaacttcat gttgagagag ctggctgctg atataactcc ttcgtttgcg
      961 gcgttccggc agcaacgctt caggaagatg aaccacacac tctgccagtc gtgccgcact
     1021 gaaggcttcc gaaaaaaaca gagcacttcc cagatgcgtg tggaacagcg cctgcatcag
     1081 gatcaccaga aacattgcca tctgccggtt gtccagtttg atgaggcgtc cttccacctg
     1141 cagtgacaga ctttcaggtt tcagcagggt atacaactgc gcacttttct gttgcgcata
     1201 acggggcgta ctgaccgccg ctgacagaac ttgatgataa gccgccagcc cgtcattacc
     1261 ggtcagcaag ggatctgcac cacgctccag cagtaactga accagtgcaa tattacctgc
     1321 ccgggctgct gacatcagcg gagtaagatt aaacggcgaa cggtgatcaa caccataacg
     1381 ttcaatatcc cgcaacacgg caaccgggtt attcattgag tatggccgga aatacttcag
     1441 ctccagtaac tgcagagtct tcgttctggg ctgccttgtg ggcttaaaat cttcccggta
     1501 cagtgcatta tacagcggga acagggaata aatcagactg tactccagcg cctgcagttg
     1561 tatggtttta tcggctgtac ctgcgaggat atgctgtttc cactgtcgtg catcctctgc
     1621 ggtgataatc tgccatggca tctcagatgt ctgcaggatc cggctacgga tggtgtccgc
     1681 ctgctcctgc ttcccctgtt tttcaagcag atgtgcggtt ttctgccact catcacggct
     1741 ggaaatctct tcctgcagat tcagaggctc ttcctgatgc gtgagcgcaa gcagactcca
     1801 cagaggatgc tccacctgct gttcaacaag atacacattg tgtttagcac gggtgagcgc
     1861 aacatacagc gcgttggtaa aaaatttata gatttccgct gaacgatcct gcctgtcccg
     1921 cggacgggaa taacgggcct cgccctcaag atcagccggt gtaagaccct cgcaaatatc
     1981 atcaaatgcc tgccgggcag cgcttactat attgtaaagg atcaccgttt cgtattccag
     2041 ccccttaacc tcctgaaccg aaaagaccag cggcgtactg aaccagcatc gcgcctgtgc
     2101 tttctgttcc ggatgcatca caatgaccgc tacacgattc gaaagtgagg ttttggcatt
     2161 cagttcctgc tttgtctctt ccctatcgtc cagcaaccgg atggtccctt ctgcctgacc
     2221 gcagcttctg acaaaatggt gactttcccg atcaatagca ctaaagcgga cctgtttgag
     2281 tctcagcaac cggttagcca gtgcagtgac gtggtgcccg ttacggtaat tcgcctgcag
     2341 gatattaacg gttgtgtcgt ttccctgttg ctgccggaaa aagagacttt tgagcgaact
     2401 ccatgagaaa aagctcggat gaactatctg attggcatca ccacagagta aaaactgacc
     2461 gggatgacgt agcgttttca gtaccagttg cagttgaaca ggcgtcatat cctgaacttc
     2521 atcaacaaaa atgacatcat aacggggagt tgccaacgaa agatactgat gactgagcag
     2581 attactgtca tactgatgcg actgtttcag ccatgcgata taacgttcaa acaggacata
     2641 gaccgtcggg cggtcctcca tgccatagag cgactgccgt atccccagtg acagatacgc
     2701 ctcccgggac agcggcccat tcccggaggc aaccgcaccg atcacgcccc tgaactcctc
     2761 atacagggta tgggcagccc ccagtgcggc gatagccctg ttgcggggca gccagtcgct
     2821 gaacgcactg aaagtcacct ccctgccttc cggaatgcgc agagtttcca gaaattccgt
     2881 cagcgaaaga aagtcaatat tctgtacctc actcccttcc cctgaagcgt catagagagt
     2941 acgcgctttt tccaccagaa atgaggaaag gctgagatac agtatatcac cagcagcctg
     3001 cttcattttt tccagtacca gcgccgtctt accacttccc gccgagccaa caatcaccag
     3061 cggtagcggg tgttcgtaca gtgcctgctg atcatcatca aaggacagca ttttatccag
     3121 gcggtggaaa cgcccatgag acggattgat atacgtcagc gcttcagtcg cgatatcaac
     3181 aggatccggc actggttgtt gttgtaagcg cccttcatca atcaccacgt ttctgcgaag
     3241 aaaacgtgaa gtgttatagg catgattgcg aatatattcc agaacaaggc agatagtttc
     3301 accgtggtag cgatacagcg aaaacagtaa tcggtcccgg atactgagcc gggcgcggta
     3361 aagattggtg tcaattttac gaacatcagc ctgaacaaag ttatcggtgg caatagcctg
     3421 tgcaaatttt ttataacccg ggatcgtgtc aggattcaga gtatgataaa gacagatttt
     3481 catggctgcc ggtttccgtc gtgcaaaaaa catgctgatt ttacgaagga atgccttact
     3541 gaacaacctt gcccggaata ccccccgaaa tcagatggaa caatcagaaa aatgctctgc
     3601 agttatggaa taaagaaggt ggggtggtac gccgggaggg cgatccgctg gaaatggcgc
     3661 agtagctgca ggcctgtcat gatgcaggaa tagaagttcg tgtacaggtt aatgaaagcg
     3721 tcaccccgta aataaactct gcgctgaaat tccttttttt cattggagta cctgtgttgt
     3781 tctgaggtga acatatcacc tctgttcagg tagccaaatt cagtgtgcca cctcaaggtt
     3841 cttagtgagt cgtttttaaa agatatgata atatatcatt gtttatgaat tgttgatgtt
     3901 taatttagta aattatatca aatatgccat gggtttgcta tgttattcat ccagtatctg
     3961 gaaataaaat gcaccagctt tattgggttt tccttttgtc tctgcttgct aggtgccaga
     4021 aactttaaat gttaaacgaa gggggcattt tgttcaaagc caatcatgtt ccggtattaa
     4081 tgtatcatca tgtcagtcat tgccccggac tggttacact ttctcccgtg acattccgga
     4141 agcaaataaa atggttggct gaaaacaact ggaaaacact gtcatcagat gaactggagt
     4201 ttttttatcg gggcggaaaa ctcccccgga aaagcgttat gttgacattt gatgatggat
     4261 accttgacaa ctggtttcag gtatatcctt tattaaaaga gtttaatctc aaagcacata
     4321 tttttttaat taccggtttt attgggaatg gtccggttcg tcactctcct ggaaaggaat
     4381 attctcatcg cgattgcgaa catcaaatag cgacagggaa tgctgataat gttatgcttc
     4441 gctggtctga agtaaacgaa atgttgcaat ctggtctggt cgaatttcat gttcatacgc
     4501 atactcatac acgctgggat aaaaagttca gttcacgaga agaacagtgt aaacatcttc
     4561 ggcaggactt gttgagcggg agggaatatt tgaaagaaat gacaggaaaa tgcagtaaac
     4621 atttgtgttg gccggaaggt tattataaca aggattatat tcaggtcgct gaagaacttg
     4681 gattctatta tttatataca acagaaagaa gaatgaatgc tcctgctaaa ggaactacac
     4741 ggataggaag aataagcacc aaagaacggg aaagttgcgc atggctgaaa agacgacttt
     4801 tttattatac aaccccgttt ttttcatcgt tgctggcctt tcataagggg ccaagattac
     4861 cggatgatta ataatgaata tactatttac ggaatcatca ccgaatatag gtggtcagga
     4921 attacaggct gttgctcaaa tgaaggccct gaagaaaatg gggcattcag ttctgcttgt
     4981 ctgcagggaa aacagcaaaa ttgcttttga agccagtaaa ttggggattg atatcacatt
     5041 cgcgttattt cgaaacagtc ttcacatccc tactgcatgg agattactcg gaatagttca
     5101 tggttttcag cccaatgcaa tcgtttgtca cagtgggcat gatagcaata ttgttggttt
     5161 agtacggtta ttcactagga aacatccatt cagaattatc aggcaaaaga catatttgac
     5221 acgaaaaaca aaagtttttt caataaatca tttttgcgat gaggtgattg ttcccggaac
     5281 aagtatgaag acacatctgg agcaggaagg atgtcgaacc cgggttactg ttgtgcctcc
     5341 aggctttgat ttccagaaat tatacgttga ttcccgaaac agtttgcctc caaatgttct
     5401 ttcttggctg gcgtcccgaa ggggatgccc cgttattgct caggtaggaa tgttgcgccc
     5461 ggaaaaaggg cacgaattta tgttgaattt actgttccat ttaaaaatga atggacgaca
     5521 gttctgttgg ttgattgtgg ggtctggttc gcctgaactg cgggagcatt tacagtatca
     5581 gattgacagt atgggcatgc atgatgatgt ttttattgct gacaatgttt ttcctgccgc
     5641 ccccgtatat cgggttgcca gtctggtggt tctgccttca gaaaacgaat cgtttggtat
     5701 ggtactggca gaagcatcgg cattttctgt gcctgtactg gccagtcaga ttggtggaat
     5761 ccctgatgtt attcagaata accagaccgg gacattgtta ccagcaggta ataagcacgc
     5821 atggatgtgc gccctgaatg atttttttaa tgaccctggg cgtttttatc agatggctcg
//
LOCUS       DAZOYF010000208         5744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZOYF010000208 DAZOYF010000000
VERSION     DAZOYF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.210"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..173
                     /locus_tag="ACHUOF_003810"
                     /pseudo
     CDS             1..173
                     /locus_tag="ACHUOF_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(489..827)
                     /locus_tag="ACHUOF_003811"
     CDS             complement(489..827)
                     /locus_tag="ACHUOF_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612798.1"
                     /translation="MVAETMFPNIAGSRSCIDCHHVAGRTFSKSARRIRGAPLLRRTS
                     RYDAQTSAFGISNDFRCISYVIPFWVVSSAMNVIPIPSLQTHYRSFITTTNGSAPVSR
                     YVPETGSHVP"
     gene            749..1876
                     /gene="ltrA"
                     /locus_tag="ACHUOF_003812"
     CDS             749..1876
                     /gene="ltrA"
                     /locus_tag="ACHUOF_003812"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR0612799.1"
                     /translation="MFDQQRDGNLYKIWNRLCSGTWFPPPVLEKRIPKSNGKERILGI
                     PTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDALKQCAIRCWRYSWILEVD
                     ISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSCENGELITRTRGTPQGGV
                     ISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRMSDATRLKNRLSERFSEV
                     GLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNFNGERYRKCMPGASNAAM
                     RKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRL
                     LKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE"
     gene            1959..2138
                     /locus_tag="ACHUOF_003813"
                     /pseudo
     CDS             1959..2138
                     /locus_tag="ACHUOF_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2165..2650)
                     /locus_tag="ACHUOF_003814"
     CDS             complement(2165..2650)
                     /locus_tag="ACHUOF_003814"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR0612800.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
     gene            complement(2906..3886)
                     /locus_tag="ACHUOF_003815"
     CDS             complement(2906..3886)
                     /locus_tag="ACHUOF_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR0612801.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3886..5085)
                     /locus_tag="ACHUOF_003816"
     CDS             complement(3886..5085)
                     /locus_tag="ACHUOF_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0612802.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5259..5393)
                     /locus_tag="ACHUOF_003817"
                     /pseudo
     CDS             complement(5259..5393)
                     /locus_tag="ACHUOF_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5407..>5744)
                     /locus_tag="ACHUOF_003818"
     CDS             complement(5407..>5744)
                     /locus_tag="ACHUOF_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0612803.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1574 a         1317 c         1368 g         1485 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtagagg tgggccctcc ggtcagggtt tacaggagca
      241 cagaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg
      361 aagcatcgaa atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc
      421 agaagacacc tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc
      481 agaccatctc agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac
      541 ccattcgtag tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca
      601 ttcattgcag aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt
      661 gaaataccaa aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc
      721 ccccggatgc gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata
      781 caagatctgg aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg
      841 gatcccgaag tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat
      901 tgctcaggga gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga
      961 ttcatatggt taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg
     1021 gtgctggcgt tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag
     1081 acatgatctg gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta
     1141 ctgtcgacgc tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg
     1201 aacacgaggc actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca
     1261 ctatgcattt gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc
     1321 tgacgatatt gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt
     1381 atcggagcgc ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta
     1441 cattgacacg tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt
     1501 caaagtgcgt acgctgaaga atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc
     1561 gtcaaatgca gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc
     1621 aacagctgag agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat
     1681 cgagtactac ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca
     1741 gtcacgtctg ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg
     1801 aaagctgacg ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg
     1861 tgcatcaaat gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag
     1921 cagccgggga gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg
     1981 aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag
     2041 aaaaatggct gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg
     2101 atctgttgcc ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag
     2161 ccgcttactt ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa
     2221 gaagtccacg aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat
     2281 tcaaaaaagt ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc
     2341 catgttggca ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct
     2401 gataagcttt aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt
     2461 cccgggccca gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt
     2521 gcaccagcca gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat
     2581 gagcattgat actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct
     2641 gttcagacat ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg
     2701 aaggctgccg tgaaagaggg ggaagactat ctcatcggtt actgttaaac ggctaagaga
     2761 aactatcttt ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg
     2821 ttcaaaaaaa acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggcg
     2881 agttgctggc gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag
     2941 caatcatacg atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga
     3001 acgtacgtcc ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca
     3061 aagttacaac cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta
     3121 taagccgcat aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa
     3181 gctcaatact cggcgagata ttcttcacaa cttcatcaat agatatatca cccagcctca
     3241 gtcgttcact taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag
     3301 gaaaaagaga cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag
     3361 ctgccgacaa tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac
     3421 gtactaaacg caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg
     3481 ccagatgctg agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa
     3541 ccttgcatag caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc
     3601 cagtccgaat cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt
     3661 ttagtgagtc cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag
     3721 tttgagtatc cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag
     3781 tattgagggt aaatacatga actggagccg atatttcttc agtattattc aggatatttg
     3841 tattcagcgt acggccaata gtcggtcggt gctttctgtt ttccatcagt agttggccct
     3901 tataaattca atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt
     3961 tttcaaagct tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa
     4021 cgactcgccg cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc
     4081 accgaaaatc tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt
     4141 cgacatgaag ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg
     4201 tacaagttca ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg
     4261 cggcaccggt gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag
     4321 atgcgggcct gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt
     4381 ctcacgcaat atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca
     4441 gtttgatgca ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac
     4501 tgacggaacg ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg
     4561 cggtgcggtt gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga
     4621 tgactgggga tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg
     4681 agcacgcagg gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag
     4741 actaccgatg aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg
     4801 atgtgcatag atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc
     4861 tgggcgtttt tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct
     4921 tgcgcgcgtt aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac
     4981 atcagcctga aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt
     5041 atctgcacgc tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta
     5101 tgtaggattt taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtaca
     5161 tattgtacat gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa
     5221 tattactgta agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc
     5281 agttcaccac cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg
     5341 gaaacatgtt ccggtaacgg acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc
     5401 ttccaatcag tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc
     5461 cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt
     5521 ccggttgtta cgtgcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg
     5581 cacttctctg gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt
     5641 caacagggct aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa
     5701 cacaccgcca gttttagtgt tgtacgcata ccagagccag tgtt
//
LOCUS       DAZOYF010000209         5738 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZOYF010000209 DAZOYF010000000
VERSION     DAZOYF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.211"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..241
                     /locus_tag="ACHUOF_003819"
     CDS             <1..241
                     /locus_tag="ACHUOF_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612804.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="ACHUOF_003820"
     CDS             complement(238..1179)
                     /locus_tag="ACHUOF_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFR0612805.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="ACHUOF_003821"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="ACHUOF_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="ACHUOF_003822"
     CDS             complement(1684..2904)
                     /locus_tag="ACHUOF_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR0612806.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="ACHUOF_003823"
     CDS             complement(2908..3651)
                     /locus_tag="ACHUOF_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR0612807.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="ACHUOF_003824"
     CDS             complement(3542..5008)
                     /locus_tag="ACHUOF_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFR0612808.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5738)
                     /locus_tag="ACHUOF_003825"
                     /pseudo
     CDS             complement(5008..5738)
                     /locus_tag="ACHUOF_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
BASE COUNT         1226 a         1618 c         1435 g         1459 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAZOYF010000210         5702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZOYF010000210 DAZOYF010000000
VERSION     DAZOYF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.212"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHUOF_003826"
     CDS             complement(<1..170)
                     /locus_tag="ACHUOF_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612809.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="ACHUOF_003827"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="ACHUOF_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR0612810.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="ACHUOF_003828"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="ACHUOF_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHUOF_003829"
     CDS             complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHUOF_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR0612811.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHUOF_003830"
                     /pseudo
     CDS             4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHUOF_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
     gene            5484..>5702
                     /locus_tag="ACHUOF_003831"
     CDS             5484..>5702
                     /locus_tag="ACHUOF_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFR0612812.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAZOYF010000211         5604 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZOYF010000211 DAZOYF010000000
VERSION     DAZOYF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.213"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..248)
                     /locus_tag="ACHUOF_003832"
     CDS             complement(<1..248)
                     /locus_tag="ACHUOF_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0612813.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
     gene            complement(335..696)
                     /locus_tag="ACHUOF_003833"
                     /pseudo
     CDS             complement(335..696)
                     /locus_tag="ACHUOF_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1035..1589)
                     /locus_tag="ACHUOF_003834"
                     /pseudo
     CDS             complement(1035..1589)
                     /locus_tag="ACHUOF_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1732..1854
                     /locus_tag="ACHUOF_003835"
                     /pseudo
     CDS             1732..1854
                     /locus_tag="ACHUOF_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="ACHUOF_003836"
     CDS             complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="ACHUOF_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFR0612814.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2964..3278)
                     /locus_tag="ACHUOF_003837"
     CDS             complement(2964..3278)
                     /locus_tag="ACHUOF_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612815.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3356..3466)
                     /locus_tag="ACHUOF_003838"
                     /pseudo
     CDS             complement(3356..3466)
                     /locus_tag="ACHUOF_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="ACHUOF_003839"
     CDS             complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="ACHUOF_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFR0612816.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4839..4976)
                     /locus_tag="ACHUOF_003840"
                     /pseudo
     CDS             complement(4839..4976)
                     /locus_tag="ACHUOF_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5058..5356)
                     /locus_tag="ACHUOF_003841"
                     /pseudo
     CDS             complement(5058..5356)
                     /locus_tag="ACHUOF_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1517 a         1226 c         1133 g         1728 t
ORIGIN      
        1 cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata
       61 taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc
      121 tcagattttg cctgcaaata acgtttaacg gtattgcggg agagccccag ttctctggca
      181 atcgcccggc tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa
      241 gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg
      301 ttgggtgtgg atcactattg caccgttcgt gacagtgttg ttgcgccttg ctgccaacga
      361 agctccactg cccatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag
      421 atgttatccg ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt
      481 cagcgtacgg ccagtatcgc gaactccggc accgttgaaa gccatttcag tgatttggtt
      541 tttaactccc ggtttacggg cctcgtaaca gtaagtgagc tgaaacaccc ggtgacagtc
      601 acggcaacgg aatcgatcat gtccttttgg gttctgcccg tgacggtaaa cctgaacgga
      661 ctggcaacgg gggcaatgaa agttaactgt gaccatgaaa gaacctaaaa gcgggtatta
      721 taacccctaa ttcaactaat tggaggcatt gtcattttta atctatggat acacttaaaa
      781 atcctctcac cttacgttaa aaaccttctc atgtttagag ttttttatta tgtttatcat
      841 aatgatatga cgatctgagt ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac
      901 agtgcctgtg gataactttg cgtaaatccg gaggacagat cactctgaag caagacaaca
      961 gcatctgaaa acaatatgct cacgtttcaa ccacagggcg taagtgatcc tacccacgta
     1021 atatggacac aggcctaagc gaggttctgg ttttcaaatt gttccggact gaggccgcca
     1081 caccaactgt gccgtcgcca gcgattgtaa tcacattcga tataattaaa cactgttgcc
     1141 ctcattattt cccggctgat aaagtgttct ccgtggagac attccacttt cagcgaatga
     1201 aagaagcttt ccacgcaggc attatcgtag cagcagccct ttgcgctcat actgccacgc
     1261 agattatgcc acttcagcag cgcctgataa tctgctgaac agtactgact tcctcggtcc
     1321 gagtgaacga taacgttccg gggtctctta cgccgccaca gcgccatctg cagggcatcg
     1381 caggccagtt gcgccgtcag gcgtggtgac atggaccagc caataacggc acgtgaccac
     1441 aggtcaatga ccacagccag atacagccag ccttcatctg tacgtaagta cgtgatgtct
     1501 cccgcccact tctggttcgg accactggcg taaaaatcct gctccagcag attttctgac
     1561 acaggcaggc catgtgcgcg gtaactgacc gtattgctgg gtaagatcaa tttgctcact
     1621 ggtatttact acgtgcatcg aatgagtgat caagagccgt atgacgcgag agtgtcacgt
     1681 acggttccgt aagcagccgg agagtgaaac tctctccggc tgactcgact cgcaggttcc
     1741 gacagcggtg acgaacatgc agcggtgttg ttctcgctga tcggcacatg ccgtctgaac
     1801 aatgtggagc cagaaaaatg gctgcgttac gttattgaac atatccagga ctgaccggca
     1861 aaccgggtac gcgatctgtt gccttggaaa gttgatctga cttctcagta aatatcaata
     1921 tgattctgac gagccgctta cctttgagca gagaaagggg agagggatcc cacatccatg
     1981 agctaggaaa atacaaaaac tggctataca acaattcaat atattaggac ttacttgcga
     2041 ggagaaatat agcaccgctc aggcaattcc tgggtattta cttttcttag tttctttact
     2101 cgggatgttc tgtcatcaga aaggcgattc taaatttgta atatagtcac tcatattccc
     2161 acgtaatttc tttagatcaa tatattgcac atttttctca aaaaactgat cggaaataag
     2221 atttgcaaca ttgcttgttg tatcattttt aacgcttcta ttcatccctt cacaaagttc
     2281 gtccattttc tttttgaaat aactgcttcc tgatacaaga tgatttatgg aactttgtgc
     2341 agcattgatg tctagagaaa tgcgataatc tttctcaacc tgtttaaata tctgtttacg
     2401 gagattatcg ccgatttttc ggctgtgttc tgcacaaaac ttatcaatac attggtttac
     2461 cacccgggat acgtagactt ttttttcatc atttttccaa tctgtaatat tttcaccgat
     2521 ttgttctgaa acagagctca aaatactagt atttattttg gttgatgata tttttactat
     2581 ccttttcccc attgtacagc ggataatctc atccaccttt cctgaaaaat atcttttgtg
     2641 agataaactg attccaaaat tattaaaaga tgttccaagc attataatca ccttgtcatg
     2701 taaaatagta acattgtcat tattttagct gattcaaaca attctgtaaa tgctttgagt
     2761 tctttactcc cttggctcaa tgatggaccg tctggaatcc cagagactaa actctctgag
     2821 aaagagagta tgaaaaaact tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat
     2881 aaccggcatc attcataatg atgccctgtt agttgtagtt atgaagattt acgtcattaa
     2941 aattgctgtt cgtagtgtca gtcctatggt ctggcatcag ctgaggatcg tagccgatac
     3001 atcactggct tcacgacatt cgtgttgagg ctattcatga aaactccacg ctgaaaacgc
     3061 cgttttgtat aaacggtcac ggtatgcccg gagccactgt tgctgataaa ttcgataaaa
     3121 ccttggcgtt tcttgaagct atcgttaatg cggataatga aacaacgatt ggtgagattc
     3181 gctcttttgc cgacgccctg gacgctgtcc ggttcaatcg caacaaaatc aaccggcagc
     3241 tgagcaaacc caaccttgcc tctctggcgc tggagcatga agtgatttgg ctgggacgta
     3301 gccgctgaca gcttctataa aagtatgatc ctacccacgt aatatgaaca caggcttaag
     3361 tgaggttctg gttttcaaat tgttcaggac tgagacctcc acaccaactg tgccgtcgtc
     3421 agcgattgta atcgcacttg atataattaa acactgtata tttcatttgt ggagtctcca
     3481 gtctgagagg gctttatgca ttccgtattg ggcactatga tgccagaaat ctgcaaggct
     3541 ccacaccttc ctgtatttcc cttcctctat cacagtgcac tttacttaat agggtgggat
     3601 gtgttcatta catttattgt gttttttatg attgtttttg cgtccagtgt cgttcgtcat
     3661 gggaaactgg ttctatgggt agtatcgctc agtctagtta actgtttgtt tctttttaga
     3721 aattaagcag atcacacaat tcggggagag atccttctaa gtgcaattct ttgcagctat
     3781 ttagcacact taaaacaact cttgtatttt gtcagaaata atattaaaca tgtcttgttc
     3841 attattgtta agtctgtctg ctatttctgt aagttttatc ccactatgat ggcatatatt
     3901 tatatatgcc atcatagcct cgatattccc tttccctgcg gccagaaata atcctggtaa
     3961 cccctcatta tctcttacat ccaatagttt aatcgtttct tttggtgata aatcaaaagt
     4021 tttaataaaa tctccataag cttgaatcgc agcggaatga ccttcttgaa gagctatgaa
     4081 caaagcagat tccccagcat tatttcgtgc tgcaagaact tctagttttt cctctttggg
     4141 tatttcggct gccaacagcg attcaccgta tttacgcgct gcatcggcat ttccatgcca
     4201 aatcaatgaa aacaaattat ggcggttata ctccttatta ttctttgtcg gaatggatat
     4261 gttctgtatt ttggccaatt ttctgaaaga aacaccacta tatccccccc ctgatttttc
     4321 ttcattgatg gcgtgggata tagcggtatt tgtcacgtta atgatattat ttttcattcc
     4381 tttattttca ttagcagtat tgctgcctat tattaagtgc atatttttgt tgtttgctgg
     4441 atgtaatcca tagttattta ttgacatttt tgttcatcct ttttgctaaa aaaaaacaat
     4501 aacctatttt tgtaatgtaa tagatattag tctattgcta atctaaaaaa accattgcca
     4561 cattgaatgg ctatattcac caataaattc tttcgatttc gtatattgac atatcgtaga
     4621 tatatcttat ccctaagatt caataatcga gtccgtttac cattctgtgc aagcgccttc
     4681 actcagaagt gaccgtcaag gcgatcacca aacttgataa taaagcagct cattgccata
     4741 cgccagtcct taaggggcgt tgtccatttc tgtgatgctg cctagattta actgccagct
     4801 ccggcttatc tacatctaaa tatgtgagcc taataccgct acactttgtc cagactatgt
     4861 ttttctccgg gaagtgggct aaccgtctcc ataaagtgac gaactttttt caacagtggc
     4921 catattgaac ggcactgatg attacgggtt attgtctcat gaagagccgg ccacatcact
     4981 gacagcagtg acttcaggcc ttcagcacca gacagcctga accagttaat ccatcgcccg
     5041 acaaatttag taatctaggg ctggtttttc ttcagctgtt gcggttcgaa agtgccatta
     5101 cgatcacgca gagtacggat ttccagaggt tcatctccag tagtgataat cttcgttaag
     5161 caaccattgc gggaattgct gccagatcta agctgatttt tctcgcaccc aagatgatga
     5221 gtcatattag cattgagtac ggtttcattt tcagcatcca atcaaactga ctgagatctt
     5281 caggattttt gagacttttg gctagttcat tagctaaagc ccgcaactta ttagatgtca
     5341 actgtttttc attcataaat tcaccttcat ttgatatcaa attaaatagt tcaacatcag
     5401 agaattatac aaacttatgt acaggctcct atgatcttcg aatcctgaag attatagtga
     5461 aaataagttt tcacgttgaa atcccacata gttagtatat gataaataat catggatgac
     5521 ctgattaacc tcttggggta gtgcttttat agaagaataa atattctact aatttaatac
     5581 atcacctaaa atagtattgt tttt
//
LOCUS       DAZOYF010000212         5590 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZOYF010000212 DAZOYF010000000
VERSION     DAZOYF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.214"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(1..279)
                     /locus_tag="ACHUOF_003842"
     CDS             complement(1..279)
                     /locus_tag="ACHUOF_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612817.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHUOF_003843"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHUOF_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0612818.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="ACHUOF_003844"
     CDS             complement(1254..1670)
                     /locus_tag="ACHUOF_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612819.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="ACHUOF_003845"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="ACHUOF_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2887
                     /locus_tag="ACHUOF_003846"
                     /pseudo
     CDS             1837..2887
                     /locus_tag="ACHUOF_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2915..3004)
                     /locus_tag="ACHUOF_003847"
     tRNA            complement(2915..3004)
                     /locus_tag="ACHUOF_003847"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHUOF_003848"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHUOF_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR0612820.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="ACHUOF_003849"
     tRNA            3996..4071
                     /locus_tag="ACHUOF_003849"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="ACHUOF_003850"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="ACHUOF_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1508 a         1326 c         1293 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct
//
LOCUS       DAZOYF010000213         5586 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZOYF010000213 DAZOYF010000000
VERSION     DAZOYF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.215"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..92)
                     /locus_tag="ACHUOF_003851"
     CDS             complement(<1..92)
                     /locus_tag="ACHUOF_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0612821.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCT"
     gene            complement(102..877)
                     /locus_tag="ACHUOF_003852"
                     /pseudo
     CDS             complement(102..877)
                     /locus_tag="ACHUOF_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(895..1947)
                     /locus_tag="ACHUOF_003853"
     CDS             complement(895..1947)
                     /locus_tag="ACHUOF_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR0612822.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(1978..2820)
                     /locus_tag="ACHUOF_003854"
     CDS             complement(1978..2820)
                     /locus_tag="ACHUOF_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR0612823.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(2807..3688)
                     /locus_tag="ACHUOF_003855"
     CDS             complement(2807..3688)
                     /locus_tag="ACHUOF_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR0612824.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(3709..4992)
                     /locus_tag="ACHUOF_003856"
                     /pseudo
     CDS             complement(3709..4992)
                     /locus_tag="ACHUOF_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(5006..>5586)
                     /gene="ycjM"
                     /locus_tag="ACHUOF_003857"
     CDS             complement(5006..>5586)
                     /gene="ycjM"
                     /locus_tag="ACHUOF_003857"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFR0612825.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         1365 a         1508 c         1395 g         1318 t
ORIGIN      
        1 gtgcagtagt acgaagcatg aaccttatcc gccacctggc ccgcgcctat tatcgcgacc
       61 cgcagcggag agcttgtcat tgcacttttc acaaagacct cttagcaggt acgcaaccag
      121 gccaacgaat cacggtacgc ctgggcagga tcttctgccc ggatacgccc ttcatacacc
      181 acataaccct gatagttatc ggcgcgcagc tgttcaaaca gcgcgtggaa atcgagggtg
      241 ccactgcccg gctggtaacg atggttatcg gcaatatgca catgaccgag caagtcacgg
      301 ttgtcgtgca gcgcctgcgc caggttatct tcttcgatgt tcatgtgata gaaatcgccg
      361 ataatctgca catgtttaag attgttttcg acgatgtaac gacgagcatc ggcgagggtg
      421 ttgatcatat gatcctgata gcggtttaac ggttcgagat acaccacggt tccggtacgc
      481 gcggcgacct gttccagtac gcgcagggaa tcactcacca gtttgcggtc gccgtccagg
      541 ctacgcggcg aggtcatcgg cggtaagcgg aaggtaaaca tgccccacgc tgccggaacc
      601 cccccacttc tgccagcgcc tcgagaatgc gttcgatctg ctttaagcca ttaagacgac
      661 gctcttcgat aaagtcacca atccagccgt catagccgcc gcaggcggtg gtcactggta
      721 aaccggtttc tttgatcgcc gctttgactt cttcgatgtt gttgaccagc agtttgccgt
      781 caatctcaaa accatcgaag cccatctctt tgatataacg aaatctctcg cggatatttt
      841 ccggaaagaa tgcctgattc tgtgtgccga ttttcatttt ttcattcctt tgaattaaaa
      901 cgtaacgccc attttgatgc tctgttccgg atgctgatcg acatactgca tgtagctttc
      961 cgggctgttg gcaaaggtca ctaccgggtc gattaaatct tcgcaattga gataaccgtt
     1021 catcagcagt tcccagcagg tttcttcaat acgcttacgg ctccagcgcg gataatccgg
     1081 gttcggttcg ctgcacgcgc gggagaaaac aattttggcg ttattgaaat gcgcttcgcg
     1141 tccgaggtta aaaccttcag caaatggttt ggcaaacgcg acataggaga tggtgccgcc
     1201 gtaggccagg ccgcgcagcg ccgattgcag cgcgtcggcg taaccgctgg tttcgataat
     1261 cacatcagcc ccctgcttgc cggtcagcgt tttgatctct ttgcctacgt cagtgccaat
     1321 ggggttaagg cagaaatcag caccgtggcg acgggcaata tcacagcgat gggcgatagg
     1381 atcgacgcca attaccaccg atgcgccagc gcgtttcgcc agttggatgg caatttgacc
     1441 gatcgcgcca agccctacca ccacgacaaa atcccctacg cgtacgttgg catcgcgcac
     1501 gccgctcatg gcaaactgcg ccgggtcgta acagacggcg tttttccagg agctgccttg
     1561 cggcattttg cgcagcttgt agttattcac tgcgttaatg atgaccgtct cggagagcgg
     1621 gccgtagccg catacgctgt cgcccaccgc gtagtctgta acgtcgctgc cgcactcgat
     1681 aatgtcgcca accaccatgt tgccaagctg gaatttgcca aactcaatgc cgcgcggcgc
     1741 atctgccgga cgcggggtga acatctgcca ctcgccgtta aagtcttcat cgataaacgg
     1801 gctggcggcg cggaagtcga ccacttccgt tccgtgtttc ggtgcgccga aacgcacgcg
     1861 gatcttcact tcattggcta aaatcgcccg atcttcatac tcaaccagcg cagcaacacg
     1921 cggtgctgtg gctactaact ttttcatgta aaactccttg taaagaaact ggccgcttta
     1981 gcccttcacg ccaccggcgg tcaaaccact cttaataaaa cgttccaaaa gcgcatacat
     2041 aatcaccacc ggcaatgcgg tcaccagcga ggccgccatc atccgtcccc agatgtaatc
     2101 aggcgtactg aacagcgcgt tcaggcccac tggtaaggtg aaattgctgg cgctggagag
     2161 gaaaatcgac gcaaacagat agtcgttcca cgccaccata aagcagtaga cgaacaccga
     2221 tatcagcccg gacatcgcca ggggcacggt gatacggaag atgatttgca gacggttgag
     2281 gccatccatc atcgccgctt cttcgatctc atcagggatg gtgtcgaagt aacttttcag
     2341 cataaacacg gcggtgggca gggtttgcgt caccatggta atgatcagcg ccatctcggt
     2401 gtcgtaaata ccaagcgcgg tgatgatttt gaacagaggc accaccagca aaatgccaga
     2461 gaacatatac accgtgtaaa agctggcgtt gatggtcatc cggcctttaa agcgcaggcg
     2521 ggaaagcgcg taagctccga gaatgccgag aaataccgcc accacggagg aaaccaccga
     2581 caccaccatg ctgttacgga agtagtcgac aaacgggaaa atcatcgggt taaaaatgtc
     2641 gacgtaatgc tccagcgtcc actgttgcgg cagcagcgta ggatgcagcg atatcgcctc
     2701 tttcgcgctt ttgaacgagg tcatcagcat cacaaaaaac ggaaacagcg tgatgatgag
     2761 aaacagcgcc agcccgcagt aaaaaccgat acgactgagc gtgcgtttat ttgttgccat
     2821 tgaggttcac ccgtttcctg gtcagcagaa tgacagcgaa aataatgatg aagagcacca
     2881 ccgagatcgc cgccgctttc cctaagtcat taaaggcaaa ggcggttttg tagagataga
     2941 caccaagaat atcgaccttg gttgtcagca gataaacatc agcgaacatg tagaacatcc
     3001 agatggtgcg cagggtcacc accgtcgcca ggaccggcat aattgcgggc agcgtgacga
     3061 tgcgaaaccg ctgccaggca ttagcgccat ccatctctgc cgcttcgtac agcgatttgt
     3121 cgatggtctg caaaatcgcc agaaacgaga taaaggcata cgggaagtag cgccagatgg
     3181 cgaacagcac caccagcaca aagctactgc ccggattatc aaaccacagc ggtgcctgct
     3241 catagagatg cagaagatcg acgccgaggt agttcacaat gccgtagccg ttgttgaaca
     3301 tgtatttcca ggcaaacacc agcgaaatgg acggcgttac gtaggagagg atcaccagcg
     3361 aacgcgcggt tttgcgcagg cggaactcgc ggttgaaaaa catcgccacg gccagcccga
     3421 gcacggtgct gcccgccacc accagcgccg tataccagac cgtcatccac agcgagtgcc
     3481 agaagccggg atcggagaga atgcgcacat agttactcac cccaacaaac gttgactcga
     3541 tgttgggatt gagcggcaga cgtaaaaaac tgatttcgat attcgacacc atcggccacg
     3601 ccaccagacc gcccagcagt aataagctgg gcgcgagaag cagcagcgca aacggcatat
     3661 cggaacgacc tgaaaaaagc ctgttcattg cggattcctg tcctgggttt agtgctgttc
     3721 gatcagttca tccagctttt tctggctcgc ttgcagcgtg ccagggagat cggctttacc
     3781 cacggtgacg ttatgcacca tggagctcac cacaccagaa cccgtcacat cgcccatgcg
     3841 ggtaaagttt ttatccccta ctgcgccaaa gacctgaata tttggcagtt caccgattag
     3901 ctgattcggt agttcaccca gcgccttaat aacgtcgttg tctttccagg tggcggtagt
     3961 gacaaccgct ttattcaccg gcagcgcagc acctggcgac atcatcaccc agtcggcaat
     4021 gttgtccgcc tgctccataa aggtgacaaa tttttctgct gcttcggtct tttgtccggc
     4081 ggtaatggtc agcgaggtca acatgccgta gaccgcagag tttttctcgg ttggcaccac
     4141 gaaaccgacg tttttcgggt cgccttcttt aatcacagcc ggaaggatat aggtggagta
     4201 aatcgccatc ggcgcggtgc cgttcataaa ggcgtctttc acttccatga tgtcgttaga
     4261 acccggcatg gtgttggcag caaggtcgcg gtaataggtc agcgcctgca tcatctctgg
     4321 tgtatcaagg gtgattttgc cttcggcgtt aaagacgttg ccctagttgg ataacgcaaa
     4381 ctgggagaag gattgttccg tcagcacgct ttctgccgta ggcagcgcaa taccgtattt
     4441 tttattcgcc gggtcattca gtttctgtgc aacgtccagc agctgttgcc agtttttcgg
     4501 ctcctccagc cccgcttttg ccagcacatc tttgcgatac cagatgccgc caatccaggc
     4561 gctgacagga acaccggtcc atgcgctacc atcttcggta cgcacaatac gcagtacgcc
     4621 atcgtaaaac gcgccttcac caacgttgct gatgaccgtg gcaaccgctt tgcgatcgat
     4681 aagctgctct ttatccataa ctttggcgta gtcatggctg gtttcgatca cttccggcag
     4741 cgagccgcta cgtgaaaggg tgatgacttt ggtgttatga gcgtcttctt ccacgggcac
     4801 ttgtttaacg gtgatacctg ggttttcttt ttcaaaacgg gcaatcaatt tgctgataac
     4861 tgcctgacgc tcctgctcca ccgaagaatg cataaattcg atggatacat gggttttatt
     4921 ttcttcttta cagcctgaaa tcagggcgca ggaaaccagt gctgataaca gcacgatttt
     4981 tgatttaatc atgagatgtt cctttttatt taatccacat aacctgccag gggcgcagcg
     5041 ttatttcatt acccaatata tcaacttcac taatcagatc ccgaccatgc agatcagtaa
     5101 tatttacatg ctgaatattt ttactgacat taaacaatcc agtcagacaa ttaccatcag
     5161 cgttacttct ttgaatacgc attacggatg aattaaccgt atcgatggta aaattattat
     5221 ccggatgaaa ctcgttatgg ctgcgacgaa gtgtaattaa acgcgacaac tcatgatata
     5281 ccgcatgcct taatgtagct tcatcgttca gttctcgtgt tatctcttca ctgtgatatt
     5341 ttttacggtt aatcgcacgg ttatatccga gtttttcgac acctgcgtaa tcattacgcg
     5401 agccaagaat actttgaata tatatcgcgg gtacaccggg gaaacttaac aaaatcgcat
     5461 gggcaaggat aaacctggtg caacgttctt catcgctact ctcgcggcgg cttaacgcat
     5521 tcatataggt gacattcatc tcatagggac tgcgcgtgcc gtcgggatta tttttccagt
     5581 ttacta
//
LOCUS       DAZOYF010000214         5558 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZOYF010000214 DAZOYF010000000
VERSION     DAZOYF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.216"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..642
                     /locus_tag="ACHUOF_003858"
     CDS             <1..642
                     /locus_tag="ACHUOF_003858"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HFR0612826.1"
                     /translation="VVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAIT
                     TGKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQN
                     AVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA
                     LGLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(696..1301)
                     /gene="azoR"
                     /locus_tag="ACHUOF_003859"
     CDS             complement(696..1301)
                     /gene="azoR"
                     /locus_tag="ACHUOF_003859"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR0612827.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1502..5404
                     /gene="hrpA"
                     /locus_tag="ACHUOF_003860"
     CDS             1502..5404
                     /gene="hrpA"
                     /locus_tag="ACHUOF_003860"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR0612828.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1540 g         1282 t
ORIGIN      
        1 gtcgtaggcg cgtgcattgt gttgatggag ctaatgatga tggttcagtt atggtggtca
       61 gtctggttat gttggccagt attatcgcta ctcattattg gccgtgggtg cggtgggctt
      121 ggcgcgataa caacagggaa agatagtcag gggaaactcc cgcccgccgt ttactggctg
      181 acattgccct ggcgcatcgg gatgtggcta tctatgcgtt ggttttgtcg tcgcctggag
      241 ccggtgagca aaatgactgc tggtgtttat ttaagggcgt ttccacgaca tattccggca
      301 cagaatgcgg ttctggacgt cacctttgaa ttccctcgtg gacgagcgac aaaagatcga
      361 ctctatttct gtgtaccgat gttggatctg gtggttccgg aagaggggga gctccgacag
      421 gccgtggcga tgctggaaac attacgcgaa gagcaaggca gcgttctggt ccattgcgca
      481 ttgggattat cgcgcagtgc gctggtagtg gcggcatggc tgttatgtta cggacactgt
      541 aaaacagttg atgaagcgat tatctttatt cgagccagac gctcgcatat tgtgcttaag
      601 gaagagcaca aagcgatgtt gaaattatgg gaaaacaggt aagtggattg agatgtggac
      661 tgaatatcta cagtccacat caagaccgtg tccggttatg cagcaacaat gctgtcgatg
      721 gctgcttttg cgtcagactg tgctttcgct gccatttccg gaccgtatgc gatcccttcg
      781 gcgaagacaa atttcacatc ggtaatgccg ataaagccga ggaacgtgga cagatacggc
      841 gtcaccaggt ccgttggtcc atctttgtgg atcccaccgc ggctggtaat aacgatggct
      901 tttttacccg ttaccagacc ttccggaccg ttctcggtat agcggaaagt aacgcctgcg
      961 cgtgcaacca ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt atacatcggt
     1021 gccgcaataa cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc
     1081 agagcttcct gctgacgcgg agtcagcggc gcatcgctcg gacgcagagc gccaaccagt
     1141 tcgccatcca gtaccggaat cggatttgca gctaggtcgc gaacggtgat ttcatcagcg
     1201 gagtgctttt cgctccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac
     1261 cctgccagga tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatgt
     1321 ttgaatggac gatgccccgt tgcttgctga cactttattc acaatcctgc cacagagatc
     1381 gcgcaataaa tcgaagtcta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt
     1441 ggtactctat atctatcatt taaaagaaaa ttaatcaggc agactactgc ccactaacgt
     1501 tatgacagaa caacaaaaat tgacctttac tgccttgcaa cagcggctgg attcgctgat
     1561 gctgcgcgac agactgcgtt tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc
     1621 tgatgcacaa caggccattt tccaggagat ggcgaaagag attgaccagg cggcagggaa
     1681 agtcctgctg cgtgaagcgg cacgaccgga aatcacttat cctgacaatt taccggttag
     1741 tcagaaaaaa caggacatac tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg
     1801 ggaaaccggc tccggtaaaa cgactcagct accgaaaatc tgtatggagc tggggcgcgg
     1861 tattaaaggg ctgatcggtc atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa
     1921 ccgtattgcg gaagagctga aaacggagcc gggcggttgc atcggttaca aagtgcgttt
     1981 cagcgatcac gtaagtgata acacgatggt caagctgatg accgacggga tcctgctggc
     2041 ggagatccag caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcgca
     2101 cgaacgcagc ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg
     2161 tcctgaccta aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca
     2221 ctttaataat gcgccgatta ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta
     2281 tcgcccgatt gttgaagaag ccgatgacac cgagcgcgat cagttgcagg cgatttttga
     2341 cgccgtagac gaactaagcc aggaaagccc cggcgacatt ctgatcttta tgagcggcga
     2401 gcgggaaatt cgcgataccg ccgatgcgct gaacaagctg aacttacgcc ataccgaaat
     2461 cttgccgctt tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag
     2521 cggacggcgc attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg taccggggat
     2581 taaatacgtc atcgaccccg gtacagcgcg tatcagccgc tacagctatc gcaccaaagt
     2641 gcagcgtttg ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg
     2701 tggtcgtgtg tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctctcgcg
     2761 cccggagttt accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat
     2821 gaccgcgctg gggctgggcg atatcgctgc gttcccgttt gtcgaagcac cggataaacg
     2881 caatatccag gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca
     2941 ggccagcgcc tataaactga cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc
     3001 acgtctggcg cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat
     3061 tatcacgtcc gcgctctcca tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc
     3121 atcggacgaa aaacatcgtc gcttccacga caaagaatcc gacttcctcg cgtttgtaaa
     3181 tctgtggaat tatcttggcg agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct
     3241 gtgtcgtacc gattacctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca
     3301 gttgcgccag gtggtgaaag aacttggcat tccggtcaac agcgaaccgg cggagtatcg
     3361 tgaaattcac atcgccttac tgaccggttt gctttcccat atcggcatga aagatgccga
     3421 taaacaggaa tataccggtg cacgtaacgc gcgtttctcc atcttccccg gttctggttt
     3481 attcaaaaag ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg
     3541 ggggcgcatt gctgcgcgta tcgacccgga atgggtagaa cccgttgctc agcatttgat
     3601 taaacgcacc tacagcgaac cgcactggga acgggcgcag ggcgcggtga tggcaacgga
     3661 aaaagtcact gtttatggtt tgccgattgt tgctgcgcgc aaggtcaact acagccagat
     3721 cgattcggcg ttatgtcgtg aactctttat tcgccacgct ctggtggaag gtgactggca
     3781 gacgcgtcac gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga
     3841 acacaaatca cgtcgccgcg atattctggt tgatgacgaa acgctgtttg agttctacga
     3901 ccagcgcatc agccacgatg taatctccgc tcgtcacttc gatagctggt ggaaaaaagt
     3961 cagccgcgaa acgcctgatt tgctcaactt tgaaaagagc atgttgatca aagaaggggc
     4021 ggaaaaaatc agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg
     4081 tttgagatat cagtttgagc caggcgcgga cgcggacggt gtgaccgtac atattccgct
     4141 gccgttactt aaccaggttg aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg
     4201 cgaattggtg attgctctga ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc
     4261 tgcaccaaac tacgccgaag cgtttttagg ccgcgtcaca ccgctggagt taccgttgct
     4321 cgacagcctt gagcgcgagt tacggcggat gaccggcgtt accgttgacc gcgaagactg
     4381 gcactgggat caggtgcccg atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa
     4441 caagaagcta aaagaagggc gctcgctaca agatctgaaa gatgcgctga aaggcaaagt
     4501 gcaggaaacg ctgtccgcgg tggcggatga cggtatcgag cagagcggct tacatatctg
     4561 gagttttggt cagctgccgg aaagttacga acagaagcgt ggcaactata aagtgaaggc
     4621 gtggccggcg ctggtggatg aacgcgacag cgtgacgatc aaactgtttg ataacccgct
     4681 ggagcaaaaa caggcaatgt ggaatggtct tcgccgtcta ctgctgctaa atatttcctc
     4741 gccgatcaag tatttacatg agaagttacc aaacaaagcc aagctggggc tgtactttaa
     4801 cccgtatggc aaagtgctgg agctgatcga cgactgtatc tcctgcggtg tggatcaact
     4861 gatcgacgcc aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa
     4921 agtgcgcgcc gaactgaatg acacggtggt ggatattgcg aagcaggtcg agcaaatcct
     4981 cactgcggtg ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcact
     5041 ggggctttct gatattaaag cgcagatggg cgggttggta tatcgcggtt ttgtcactgg
     5101 taacggcttc aaacggctgg gcgacacgct gcgatatttg caggcgattg aaaaacgact
     5161 ggaaaaactg gcggttgatc cacatcgcga tcgtgcacag atgctgaaag tcgaaaacgt
     5221 ccagcaggcg tggcagcaat ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga
     5281 cgtgaaagag atccgttgga tgatagaaga gctgcgcgtt agttacttcg ctcaacaact
     5341 tggtacgcct tatccgattt cagataagcg tattttgcag gcgatggagc agattagcgg
     5401 ttaaccctgc tatttgccta ataaatctaa aacccggtaa gcatttagcg ccgggttttt
     5461 tattaattct aaaacggtaa ggacaaaaat tcaggaattc agaaaaatac aattctcagc
     5521 tgcaagatga ataatgttta tctacagcat ttccttaa
//
LOCUS       DAZOYF010000215         5550 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZOYF010000215 DAZOYF010000000
VERSION     DAZOYF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.217"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            223..882
                     /locus_tag="ACHUOF_003861"
     CDS             223..882
                     /locus_tag="ACHUOF_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR0612829.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1083..1460)
                     /locus_tag="ACHUOF_003862"
     CDS             complement(1083..1460)
                     /locus_tag="ACHUOF_003862"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0612830.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1527..4493)
                     /locus_tag="ACHUOF_003863"
     CDS             complement(1527..4493)
                     /locus_tag="ACHUOF_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR0612831.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4496..5056)
                     /locus_tag="ACHUOF_003864"
     CDS             complement(4496..5056)
                     /locus_tag="ACHUOF_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0612832.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5182..>5550)
                     /locus_tag="ACHUOF_003865"
     CDS             complement(5182..>5550)
                     /locus_tag="ACHUOF_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HFR0612833.1"
                     /translation="RLLKTTMEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAA
                     FVGFVNQWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQAR
                     ASTATETSVKPDACDSPPSG"
BASE COUNT         1117 a         1532 c         1655 g         1246 t
ORIGIN      
        1 gacgcacttt gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat
       61 cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt
      121 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
      181 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      241 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      301 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      361 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      421 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      481 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      541 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      601 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
      661 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
      721 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
      781 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
      841 gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat
      901 tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc
      961 agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg
     1021 cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct
     1081 tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata
     1141 tgatcattta ttctgcctcc cagagcctga taaaaacggt tagcgccggg gttggatttt
     1201 tcagcgttcc agctaaggct aaggcattcc tgttcaagcg caagccgggc tataaagcgc
     1261 attatcgctt tacccgtgcc cttatttcga tcgcactgag aaacatacag ctctttaata
     1321 tgcagctgac cgctgtatcg gggcgaggga taaagaatat tgcaacatgc caggccagta
     1381 atgttattgc cgcagcgcgc tctgatcacc agggtaccgg aaagccggtt gaataacttt
     1441 ttacagagat aatccttcat caacgcttcc tgaatgatac cttcgccata ataggggtcg
     1501 tctcagaaaa cggaaaataa agcacgctaa gccggttgca gaggccgtag cggcctgaac
     1561 ttccccgcgc cgatcttggc gctgctgcgc cataggtaat caccggtcag gttgatgtgc
     1621 tcccagccga gtggcgacag gtactgcaat agcgagtcat cgacggcatg accattgccg
     1681 cgcaacgcat gcgccgcacg ctccaggtag accgtgttcc acagcacgat ggccgccgtc
     1741 accaggttga ggccgctggc ccggtagcgc tgctgctcga aactgcggtc acggatttca
     1801 ccaaggcggt tgaagaacac ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc
     1861 ccggcatgca cgcggcggcg tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg
     1921 cgctcgatgc ggcccaactc gcgcagcgcg acggccaagc cgttctggcg cgggtagctg
     1981 ccgagtttcc tgagcatcag cgaggccgtc accgtgccct gcttgatcga ggtggccagc
     2041 cgcaggattt cgtcccaatg ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc
     2101 ggcttgagcg cgtcataggc ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg
     2161 tcgcggatgc gcggcgcgaa gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg
     2221 gtgaagcccg ccgtgtcggt gtagtgctcc tcgatccgca ggtcggattc gtggtacagc
     2281 aggccgtcga gcacgtaggt tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat
     2341 ggcgcgtatt ggtcggagat gtgggtgtag aaagtccgtc ctgggctgct gccatatttt
     2401 gggttgatgt gccccgtgct ctttgccttg ctagcggttc ggaaattctg tccgtccgat
     2461 gatgatgtgg tgccatcgcc ccagtgcccg gcaaagggat gccgaaactg agcgttgacc
     2521 agttcagcca acgctgtcga gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg
     2581 agcttcgcgt aggtcgtgcc ggggcaggac tcggccatct tggtcaggcc caggttgatc
     2641 gcgtcggcca ggatcgtggt caacaacagg ttcttgtcct tggccagatc gcccgatttc
     2701 aagtgcgtga agtgccgggt gaagcccgtc cactcatcga cttcgagcag cagttcggtg
     2761 atcttgacgt gcggcaggac catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc
     2821 accgccgcat ccagcggcgt gatcttcaag cccgactcgg tgatgatggc atccggcagg
     2881 tcgttggctg ccgccatgcg gttgacggtg gcaagttgtg cttccagcag cgtcagccgc
     2941 tcatgcagat attgttcgca gtcggtggcc acggccagcg gcaattcgct ggactgcttg
     3001 aggctggtga acttctcggg cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa
     3061 ccctgcaccc agatgtcgcc cgagcgcagg gagttcttca actcggacag cgcgcacagt
     3121 tcgtagtagc gccggtcgat gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg
     3181 atgaagccgg tcggtgcatc ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc
     3241 agcacctcaa tggcatcaag cacgtttttg gcggcgggcg cggcccgcag cttgagcacg
     3301 gcaaggaatt ccggtgcata gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg
     3361 aaatcgaagt catcgggttg cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa
     3421 tcccaggaca tgacggcctc gatggcggca aacgcatcgc ggcctgattg cttggcgtcg
     3481 atcagcgcct gaccgatgcg cccgtacaga cgtaccttgg cgttgatggc cttgcctgac
     3541 gcctggaact gctgctgatg cttattcttg gcagcgttaa acagcttacc caggatgcgg
     3601 tcgtgcaggt cgatgatttc gtcggtgacg gtggccatgc cctcggtggc cagcgccacg
     3661 agagtggcgt agcgccgttg cggctcgaat ttggccaggt cggcgggtgt catctggccg
     3721 ccctcgcggg caatcttgag caggcggttc tggtgaacca gccgctcgat gccggtaggc
     3781 agatcgagtg cctgccatgc cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt
     3841 ggcttggccg gagactggcg caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc
     3901 ttgagcagat cgtcgaggcg gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg
     3961 tagatgcgcc ggttagcacg ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg
     4021 gcgggcagaa tgaccgactg ccgccgcagg tgcccgatca aggcgctggc cagcacgatg
     4081 cctttgtcgg tttgcatcgc cagctcggtc agcatctgga cggcctgccg gtaatggctc
     4141 atggtgaagg gccggaaacc gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc
     4201 tgctcccgct ggccgtactc gttccagctt tcgacgccga ccttgagctg gtcggcgacc
     4261 agcttcaaca agggcgggaa cggtagttca tcgacgccca ggatgacgcc gggaaagcgc
     4321 aggtaacaga gctgcaccgc gaagcccagc cgattggctg gcccgcgccg ctgtcggatg
     4381 atcgagaggt cggtatcgtt gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc
     4441 aacgccagca ggctttcccg ctcggcggcg gacaggatgg aacgacgtgg catatttact
     4501 gatccgttct caagtattga tacagggttt cgcgactgat tccgaattca cgagcaagct
     4561 tggtcttttg ctcgccagcc tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca
     4621 gggatttctt cctgccacgg taagccccgc gttgcttggc gagcgcaata ccctcgcgct
     4681 gacgctcgcg gatcagggcg cgctcgaact cggcgaacgc gcccatcacc gagagcatca
     4741 ggttcgccat cggagagtct tcgccagtaa aactgaggtg ttccttgacg aattcgatat
     4801 gcacgccgcg ttgtgtcagc gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca
     4861 ggcgatccat gctatgcacc accacggtgt cgccggtgcg ggcgaagctt atcagcgctt
     4921 ccagttgcgg acgcttgaca tccttgccgg atgccttgtc gctaaaagcg cgatcaacct
     4981 tgacgccttc cagttgccgt tccgggttct ggtcgaaggt gctgaccctg atatacccaa
     5041 tgcgctgtcc agtcatggaa ttccctgcaa aatgtcaggg aagactctat gaccttcaac
     5101 gagatatgtc aataaattca aaattcaatc ctatcctgac gcaatttaca catggcatct
     5161 gacatcaggt tagggtatgc ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac
     5221 gctggtttcg gtcgcagtgc tggcccgcgc ctggaagcgc tgatagcact ccagcccgca
     5281 gaaatgctcc acgtactcgg ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt
     5341 gcagcacacg cagcaactgg tgctcggttc attggcgttc atggtggtgt tcctccattg
     5401 gttgacgaag ccgacgaagg ccgccgccgg catcggcctg gcgaacagga aaccctgcac
     5461 cgtgtcgcaa cccgcctgcc gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc
     5521 taccacctcc attgtcgttt tcagaagacg
//
LOCUS       DAZOYF010000216         5486 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZOYF010000216 DAZOYF010000000
VERSION     DAZOYF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.218"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1631)
                     /locus_tag="ACHUOF_003866"
     CDS             complement(<1..1631)
                     /locus_tag="ACHUOF_003866"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR0612834.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWV"
     gene            complement(1647..2542)
                     /locus_tag="ACHUOF_003867"
                     /pseudo
     CDS             complement(1647..2542)
                     /locus_tag="ACHUOF_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(2542..3147)
                     /locus_tag="ACHUOF_003868"
     CDS             complement(2542..3147)
                     /locus_tag="ACHUOF_003868"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0612835.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3318..>5486)
                     /locus_tag="ACHUOF_003869"
     CDS             complement(3318..>5486)
                     /locus_tag="ACHUOF_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFR0612836.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1582 a         1364 c         1141 g         1399 t
ORIGIN      
        1 acccacggtt taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg
       61 tgccacggct gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggctagacct
      121 gcgagtgagt tttttacctg ctcattttcg ggaaaaagct catataggcc agagacaatc
      181 gccagcgtag gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt
      241 tcaaaacgca cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca
      301 tttaactcac tgtaatcacg taacaaaata tcgcttacgg caggctcgtt tgccagcgca
      361 tccagtacat agcgtccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg
      421 gcgtggagat cggcaacggc ttgtttaatc aatatttgca aatgggtttt gcgctggcga
      481 ataccgcgcc atccaacact gttcaggtag attttgtcga ttaaacgccc gaatgcgtta
      541 ctgccttgcg gttgattttg atagacataa tccagggaac tgcctgaatc aaagccggta
      601 ctcattccga gatgcaggcc cgcagagtgc gcaccgaaca atttcatcgc ctttcgcata
      661 aagcgatacg ctaaatcaac cggcgataat ggcacgggtc caccagaaag gagccgccat
      721 cgatccgctg atggtcctgt gcggtcttca tgctgataat caaatttttg cgatttgtta
      781 gcatataagc ggcagataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca
      841 cccaatgtgt cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat
      901 ctctgataaa aatcaatctg cggttggcga tgcaccacat agtcatcacc tgatatcaga
      961 agttgcgtgg ggagcgtaat cgccgccgca tcgctaacaa tacgttcaga cgttttatag
     1021 agatcgagca agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc
     1081 gccacccgtt gccgatcgtg ggtcaaatag cgccctttca cataggaatt aataaaaaat
     1141 agaccacgca gacgatgcca taacgccagc gcaggacgtg ccagcggcac atacaattta
     1201 accttaaagg ccggagaagc cagcaccagc ccacgaattg caggtgcata atcatgaacc
     1261 catgtggcaa caagcactgc gccgacgctt tgcgcgatca ctaccacctc ttccagtccg
     1321 acctggctgt cgctggcagc aaaacggaca aattcatcga catcctgcac tgagcgcata
     1381 agagacgggc tataaccacg cggccccgaa ttttttccat gcccccgggc atcccaggca
     1441 taaaaagcag tatcaggcat cgccagttca tcaacgagat gttgtagacg accagaatgt
     1501 tcatgcccgc gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agtgggccag
     1561 tgccgataat acaatgctgt attatcactg gtggtaaaaa aatgttcccc agggatgcgt
     1621 gaattttcca ttttctctat tttccttcag taacagcagt agcgaataaa ataaaaaaat
     1681 accggagcgg tgaaaattaa tgagtcaatc ctgtcaagga gtccgccatg tcctagtaat
     1741 agttttccac tatctttaac accaatatct cgtttgatgg ctgacatcac tacatcgccg
     1801 caaaaaccac taataccaat taacaaaccc gctaataatg cctgtaatgt atttagcggt
     1861 gtcagcaacg gtccaataat cagtgacgca atcatggtgg tgattacgcc ccccagcaga
     1921 ccttccagcg tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc
     1981 ccccataaat actgtgcaat atcgtttgac tcggttaacg ccaaaagaaa taacaccagt
     2041 aacgccccgc cctggatatt tatggttggc aacattaata gccaggcggc atgactcaag
     2101 gcaaaaacgg tcattatcca tccccaaaaa atggcgctca cggtatgctg aaaaccggag
     2161 gggtcaccca caaaaactcg ccaggtggct aatatcagaa acccagtcaa aggtataaat
     2221 aacagaaaca gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa
     2281 tataaccaac gcgaaaaatg tacggatata agcgtacaat attctttcaa tgccagaaaa
     2341 cttatcagcg taaaaaacgt caacgtcatc cagcgtgggc ctgaaatcac cagtgaaaaa
     2401 caaatgacga tgccccacca ggtacgaata cgtaatgtaa cttcatcccc ttttctctca
     2461 ggtaaccgcc atagcagaaa acggtttatc agcttgctaa aattaacaac gcgaaaagtg
     2521 ttgccagaga tttttccagc attatctttc agcgctcata agaagcacac tccgacaacg
     2581 attaatcgca gtacagataa gcagaattga agcaatgctc cataaaagat tattccattg
     2641 catccactgt ggataaatgg caacggccag accccacaga ccaaatatta acgcgcgatc
     2701 acttttgcca aatggaccgg cataactgcg aacaccgtta atcgtctgcg cgagtaaacc
     2761 gcaaaactcg gtcaatatgg tgcaaaataa catgagtatc acgagtgatg cgttactttc
     2821 cggtaaaaat aaaaacggta aatagagcgc aatatcggaa ataacatcgc cagtctcatt
     2881 caaaatcgcc cccagacgtg tttgctgatt gcactcacgc gccaacatgc catccagcgc
     2941 attgagcgcc atacggataa aaagcacgat gggcaatagc aaaaagagga tgggttgtgc
     3001 caccaacacc aacagcaatc cggtaaaaag agaaagcgcc agtgccgtaa gagtgatgtg
     3061 attcgctgta acgtggtgct tataaagcca aaacatcgtc ggccttaaca gcgactgaaa
     3121 gagcggtttt atctggtaga gcgtcattat caatccttga taattcggtt tgcgctatgg
     3181 tgcatcaatt cccctgacgg agatatcgca acacgccgct tcattaaaaa ttcagaatat
     3241 atcaaggtat tgggggctca ctgtcaggaa tgattatccc caataaatca agtagttaaa
     3301 aattattacg ctgttattca atacaactca tcaagtttcc tcatcgcttg ttcccgtatt
     3361 gcgcgcgtct taccgttaag cgccagctgg cggactaaat ccagttcagg gttaacaagc
     3421 tctaacgcca gcaaaggatc acgtttcagc ctcgccttca atacatcaac tggaaagcgg
     3481 ggattattca tcgccacaat ccaccactcg ggatcaattt ctgccgccag tgccgcagag
     3541 ggggtgtggc ggttgctaag tgcgccgaac ttgaaaaatg tgagattgct tttcgccagt
     3601 gtactccacc atatacgatc cggttgtttt gccagtacat ccagttgtgc taacgcatcg
     3661 gggtcgagtg cataataaac acgtaatgct tcatcaacct gcgcacggga gctattttcc
     3721 tgcctcagaa aaaacttcgc ccaaatatca ttgcgtggcg tgttttgctg taattccttg
     3781 agaacaacag cgggtaaatt atcccaacta actaatgcac tctctaaaaa gtcccagtct
     3841 tccaaacgta tcagggcggc ggcatttaat agcatctggt catcaacttc gccgtggcta
     3901 atcagctcag acaaaatcat atcagcgctg gtttcatatg catctgaatc tgctttcgga
     3961 ttatttttcc gatgcgataa agcacgttgc caaatttttt gtttcatcga cgtgctcaga
     4021 tactgatttt tcgccagatt actccacccc tcaggatcgt tgaaacgtgt caataaccgt
     4081 tgtaaagcag cgtcagagtg aattacctgc atatcccgac agcaaaagta ggcaggggat
     4141 tttttctcca tccttttaac aaaattaaac tgtaagttat ctggcaatgc taaaaataac
     4201 acacgtatta gatcatcgtc attctcgaat ttttggtata aataggctgc aactttgtca
     4261 ctctcttgtg gcgtcatccg caataattga ccatggtgtt tcatctctgc cagcttgaac
     4321 gctaagcttg tgacaacatt gtttgatgaa tcgttcgcca actgcattgc ctgattgtaa
     4381 gtgagttgtc ctccagcacc gacctgacga atagaggaat catcactgcg gatatagcca
     4441 tcaataatgg attcgggcaa taaccacgca tctttataaa gctcctgaat gatcatatcc
     4501 caatctcttt tcgccagagt catgacgata tccagaggga gattttcacg tagcgcaaga
     4561 ttcaacggac cgacaccatc aggtagacga ctcattcgcg tcattatttc gacagagagc
     4621 ggttcacggt cgagcaaccc cagaacaaca ggctggtaac ccaattgctg atcggtcaac
     4681 tccagaatct cattgtcagg catatatctc gccatacttt caatcgtttg ttcatcattg
     4741 ctattaataa gttgtttgcg taggttagct gaaattcctt taagccttgg gagagttcgc
     4801 attatggcaa gatcaaatgt cgcaatatct cgtccttcaa atgcagcaac gttgccttca
     4861 tccagcattt gcaattcctg cactaactca ggagaaggct tagggcgaaa cagcgcttct
     4921 tttttcatct gattacagac attccctgcc atgcgaatat tgcttcgctt aaaaatttct
     4981 ttcagagcat cttcatcatt tattagaccc caataatatg catcaggatt tattatcatc
     5041 agcgcttttt tgacattttc agtgtcattt ttgtcactcg cacggaccat gaattcacta
     5101 tttgtcgaag acgcaacaac aggtgcaatt atgtcattga taaatacccc acgcgcatta
     5161 atactcccct taatcgtaga cggattatcg taatatgagt cgataaatac cagtccactt
     5221 gcatttatcg aaccggtaat taacagtggt gaatccgtaa ctatattcgt tgccgtcacg
     5281 ttaccgagaa taacccatgg attagaaaga gagaaagtgg aaatattacc tttgactgtc
     5341 aaattgccaa gaacaatcaa tttttgatta ctctctatat taccttctac cactaaatcg
     5401 ccacgatgca ctttccagtc cgatgcagat aaaccgaagg ggacaagatt cacgctactg
     5461 ttgatagctt gttcaacttg ttgttt
//
LOCUS       DAZOYF010000217         5413 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZOYF010000217 DAZOYF010000000
VERSION     DAZOYF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.219"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            117..2153
                     /gene="yihQ"
                     /locus_tag="ACHUOF_003870"
     CDS             117..2153
                     /gene="yihQ"
                     /locus_tag="ACHUOF_003870"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFR0612837.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            2199..3573
                     /locus_tag="ACHUOF_003871"
                     /pseudo
     CDS             2199..3573
                     /locus_tag="ACHUOF_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            3616..5019
                     /locus_tag="ACHUOF_003872"
     CDS             3616..5019
                     /locus_tag="ACHUOF_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0612838.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            5087..>5413
                     /locus_tag="ACHUOF_003873"
     CDS             5087..>5413
                     /locus_tag="ACHUOF_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFR0612839.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         1282 a         1329 c         1356 g         1446 t
ORIGIN      
        1 aaaaacagct cagcttctgt aaagttttta tatatcagtt ttatgagaat cattttacat
       61 aagcctgtaa taacaggaag gcaacaatct cgttctcaac tctatggaga ttagttatgg
      121 atacgccacg tccacagtta ttagattttc aatttcatca gaataacgac agttttaccc
      181 tacattttca acaacgtctt attttaaccc ataacaaaga taatccttgt ttatggattg
      241 gctcaggtat agcggatatc gatatgttcc gcggtaattt cagcattaaa gataaactac
      301 aggagaaaat tgcgcttacc gacgccatcg tcagccagtc accggatggt tggttaattc
      361 atttcagccg tggttctgac attagcgcca cgctgaatat ctctgccgac gatcagggcc
      421 gtttattgct ggaactacaa aacgacaacc ttaaccacaa ccgtatctgg ctgcgccttg
      481 ccgctcaacc agaggaccat atctacggct gcggcgaaca gttttcctac ttcgatctgc
      541 gcggcaaacc gttcccgcta tggaccagtg aacaaggcgt tggtcgcaac aaacaaacct
      601 atgtcacctg gcaggccgac tgcaaagaaa atgcgggcgg cgactattac tggactttct
      661 tcccacagcc tacgtttgtc agcacgcaga agtattactg ccatgttgat aacagttgct
      721 acatgaactt cgactttagc gccccggaat accatgaact ggcgctgtgg gaagacaaag
      781 ctacgctacg ttttgaatgt gctgacacat acatctccct gctggaaaaa ttaactgccc
      841 tgctgggacg ccagccagaa ctgcccgact ggatttatga cggggtaacg ctcggcattc
      901 agggcgggac ggaagtgtgc cagaagaaac tggacaccat gcgtaacgcg ggcgtgaagg
      961 ttaacggcat ctgggcgcag gactggtctg ggatccgtat aacctctttt ggcaagcgcg
     1021 tgatgtggaa ctggaagtgg aacagcgaaa actacccgca actggattca cgcattaagc
     1081 agtggtatca ggagggcgtg cagttcctgg cctatatcaa cccgtatgtt gccagcgata
     1141 aagatctctg cgaagaagcg gcacaacacg gctatctagc aaaagatgcc tctggcggtg
     1201 actatctggt ggagtttggc gagttttacg gcggcgttgt cgatctcact aatccagaag
     1261 cctacgcctg gttcaaggaa gtgatcaaaa agaacatgat tgaactcggc tgcggcggct
     1321 ggatggctga cttcggcgag tatctgccca ccgacacgta cctgcataac ggcgtcagtg
     1381 ccgaaattat gcataacgcc tggcctgcgc tgtgggcgaa gtgtaactac gaagcccttg
     1441 aagaaacggg caagctcggc gagatcctct tctttatgcg cgccggttct accggtagcc
     1501 agaaatactc caccatgatg tgggcgggcg accagaacgt ggactggagt ctcgacgatg
     1561 gcctggcgtc cgtagtcccg gcggcgctgt cgctggcaat gaccggacat ggtctgcatc
     1621 acagcgacat tggcggttac accaccctgt ttgagatgaa gcgcagcaaa gagctgctgc
     1681 tgcgctggtg cgatttcagc gccttcacgc cgatgatgcg cacccacgaa ggtaaccgtc
     1741 ctggcgacaa ctggcagttt gacggcgatg cggaaactat cgcccatttt gcccgtatga
     1801 ctaccgtttt caccaccctg aaaccttatc tgaaagaggc cgtcgcgctg aatgcgaagt
     1861 ccggtctgcc ggtcatgcgc ccgctgttcc tgcattacga agacgatgca catacctatt
     1921 ccctgaaata tcagtacctg ttaggccgcg acattctggt cgctccggta cacgaagaag
     1981 gtcgcagcga ctggacgctc tatctgccgg aggataactg ggtacatgcc tggacgggtg
     2041 aagcgttccg gggcggggaa gttaccgtta atgcgcccat cggcaagccg ccggtctttt
     2101 atcgcgccga tagcgaatgg gcggcactgt tcgcgtcgtt aaacagcatc taatcacatc
     2161 cgcccgtggg gaaactcatg ggcaaaggga gaacatcgat gagtcacatc acaacggaag
     2221 atccagcaac tttacgcctg ccctttaaag agaaactctc ttacggtatt ggcgacctgg
     2281 cctctaacat cctgctggat atcggtacgc tttatctttt gaagttttat accgacgttc
     2341 tggggctgcc aggcacctat ggcggcatta tctttttgat ttcaaaattc tttactgcgt
     2401 ttaccgatat gggtaccggc attatgttgg attcccgacg caagatcggt ccaaaaggta
     2461 agttccgtcc ttttattctg tatgcgtcat tcccggtcac cttactggcg atcgccaact
     2521 ttgtcggcac accgtttgat gtcaccggta aaacggtgat ggccactatt ctgtttatgc
     2581 tttacggact gtttttcagc atgatgaact gctcctacgg cgcaatggtc ccggcaatca
     2641 ccaaaaaccc caacgaacgc gcctcactgg cggcatggcg tcagggcggc gctacgctgg
     2701 gcctgctgct gtgcacggtg ggattcgtgc cagttatgaa tcttatcgaa ggtaatcaga
     2761 aacttggcta tatcttcgcc gccacgctgt tttcactgtt cggcctgctg tttatgtgga
     2821 tctgctactc aggcgtgaaa gagcgttatg tcgaaaccca accaaccaat ccggcgcaaa
     2881 agcctggcct gttgcaatct ttccgcgcaa ttgccggtaa ccgcccactg ttcattctgt
     2941 gtattgccaa cctctgcacc ttaggggcgt ttaacgtcaa gctcgccatt caggtctatt
     3001 acacccagta cgtacttaac gatcccatcc tgttgtcgta tatgggattt ttcagcatgg
     3061 gctgtatttt catcggcgtg ttcctgatgc ctggcgcagt cagacgtttt ggtaagaaga
     3121 aggtctatat cggcggcctg ctgatttggg tgctgggcga tctgctcaac tatttcttcg
     3181 gcggcggctc ggtcagcttt gtggcgttct cctgcctggc gttcttcggc tcagcgcctg
     3241 aactgggcgc tggtttccga caccgtcgag tacggcgagt ggcgcaccgg cgtgcgttcg
     3301 gaaggcacgg tctacaccgg cttcaccttc tttcgcaaag tgtctcaggc gctggctggt
     3361 ttcttccccg gctggatgct gacgcaaatt ggctatgtgc cgaacgtcgc acaggctgac
     3421 cacaccattg aagggttacg ccagttgatc ttcatctacc caagcgcact ggcggtagtc
     3481 accattgtgg cgatgggttg cttctacagc ctgaacgaga agatgtatgt ccgcattgtg
     3541 gaagagatag aagcccgtaa acgcacggcg taataataat aaacgacgcc ctgcggggcg
     3601 ttataaggag tgattatgtc tgaccataat ccactgacat taaaactgaa tctgcgggaa
     3661 aaaatcgcct atggtatggg cgacgtcggt tcgaatttaa tgctctgcat cggtactctg
     3721 tatctcctca aactttatac cgatgaatta gggatgcctg cttactatgg cgggattatc
     3781 tttctggtcg cgaagttttt taccgcgttt accgatatgc tcaccggatt tttactcgac
     3841 tcgcggaaaa atattgggcc taaaggcaaa tttcgtccct ttattttata tgcagcagtt
     3901 ccggcggcgt taattgcaac gctccagttt atcgccacca ccttttgttt gccggttaaa
     3961 acgacgattg ccaccacgct gtttatgatg tttggacttt catacagtct gatgaactgc
     4021 tcgtatggtg cgatgatccc ggcaattacc aaaaacccga atgagcgcgc gcagctcgcg
     4081 gcttaccgcc agggtggtgc aaccataggg ctattgattt gtaccgtggc gtttattccg
     4141 ttgcagtcgc ttttttctga ctcaaccgtc ggttatgcct gtgcggcact tatgttctcc
     4201 attggcggct ttatttttat gatgctgtgc tacagaggcg tcaaagagca ttatgtggac
     4261 acagcgccaa ccggacataa agccagtatt ctcaaatctt tttgcgcgat atttcgtaat
     4321 ccaccattgc tggttttatg cattgctaac ctgtgtactc tggcggcatt taatatcaaa
     4381 ctggcgattc aggtctatta cacccagtat gtgctgaatg atattaattt attgtcgtgg
     4441 atgggattct tcagtatggg atgcatcctc gtcggcgtat tactggtgcc agtaactgta
     4501 aaatgttttg gtaaaaaaca ggtttattta gctggcatgg tgctatgggc ggtgggtgat
     4561 atactgaatt atttctgggg aagtaactct ttcactttcg tcatgttctc ttgtgtcgcc
     4621 ttttttggca cggcctttgt aaacagcctg aactgggcac tggtaccaga taccgtagat
     4681 tacggtgaat ggaaaaccgg tattcgtgcc gaaggttctg tttataccgg ttataccttc
     4741 tttcgtaaaa tttctgccgc acttgctggc ttcttgccag gcattatgct gacgcaaatt
     4801 ggttatgttc ccaacatcgc gcaaagcgat gcgacattac agggtttgcg tcagctcatt
     4861 tttatctggc cttgtgcact ggcaattatt gctgcattga cgatgggatt cttttacaca
     4921 ctcaatgaaa aacggtttgc attgattatt gaggaaatca accaacgcaa aaataaagag
     4981 atggcgacag aaaaaaaaac ggcttccgta acgttataaa tgtcaatagc cgctatttcc
     5041 atcctggtgg gtggcggcct ccctacgttt aaaaaatgga cttattatga aaaagattaa
     5101 tgcaataatt ttattatctt ctctgacctc tgcctcggta tttgctggcg catacgtcga
     5161 aaacagggag gcatataatc ttgcctccga tcagggcgag gttatgctac gtgtgggtta
     5221 taacttcgat atgggcgcgg gtattatgtt aaccaatacc tacacctttc agcgagaaga
     5281 tgaactaaaa catggctata atgaaattga aggttggtat cctttattca aaccaaccga
     5341 taaattaacc atccagcccg gtggcttaat taatgataag agtatcagtt cgggtggtgc
     5401 agtgtatctg gac
//
LOCUS       DAZOYF010000218         5398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZOYF010000218 DAZOYF010000000
VERSION     DAZOYF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.220"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..497
                     /locus_tag="ACHUOF_003874"
     CDS             <1..497
                     /locus_tag="ACHUOF_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HFR0612840.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="ACHUOF_003875"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="ACHUOF_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="ACHUOF_003876"
     CDS             2500..3114
                     /locus_tag="ACHUOF_003876"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR0612841.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="ACHUOF_003877"
     CDS             3114..3443
                     /locus_tag="ACHUOF_003877"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR0612842.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHUOF_003878"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHUOF_003878"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR0612843.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="ACHUOF_003879"
     CDS             4529..4840
                     /locus_tag="ACHUOF_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR0612844.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..5373
                     /locus_tag="ACHUOF_003880"
                     /pseudo
     CDS             4824..5373
                     /locus_tag="ACHUOF_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1177 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DAZOYF010000219         5374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZOYF010000219 DAZOYF010000000
VERSION     DAZOYF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.221"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(99..581)
                     /locus_tag="ACHUOF_003881"
     CDS             complement(99..581)
                     /locus_tag="ACHUOF_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612845.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(649..2598)
                     /locus_tag="ACHUOF_003882"
     CDS             complement(649..2598)
                     /locus_tag="ACHUOF_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0612846.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2683..3018)
                     /locus_tag="ACHUOF_003883"
     CDS             complement(2683..3018)
                     /locus_tag="ACHUOF_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR0612847.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(3018..3374)
                     /locus_tag="ACHUOF_003884"
     CDS             complement(3018..3374)
                     /locus_tag="ACHUOF_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR0612848.1"
                     /translation="MAKIAGICFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3374..4870)
                     /locus_tag="ACHUOF_003885"
     CDS             complement(3374..4870)
                     /locus_tag="ACHUOF_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR0612849.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4867..5028)
                     /locus_tag="ACHUOF_003886"
     CDS             complement(4867..5028)
                     /locus_tag="ACHUOF_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFR0612850.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5038..>5374)
                     /locus_tag="ACHUOF_003887"
     CDS             complement(5038..>5374)
                     /locus_tag="ACHUOF_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612851.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1284 a         1565 c         1155 g         1370 t
ORIGIN      
        1 cagtaagttg gagtcattac caagaaagag aaggtaaaga aggtaagtcc atactgtctc
       61 cactataaaa aaaccgccaa tttctggcgg cttatatatt aatcatcatt aatatctttc
      121 atattcaatt tgcaattttc gtaggtcact cccgactcgc cattaacatt aactccagct
      181 ctgatattcc cgttttcttt tacatctaca aaaaactggc ctcttggcat cttgaacgca
      241 aatgtaaaac caacagtatc tgaatcaaaa tcatcatctt tatatattgg gatcaaaaat
      301 gtcatcgtcg ctggtgatgc attgtttgca aaatcaaata cacctatact tttgccgaat
      361 tcataagaag cttttctcaa tataatcgca ccgctggcag aaggggatgg gcacgatatc
      421 ctcagttgtc tgaatccatc tgtatccaag tcaacgtcat tatctgcaga tgttttaacg
      481 ttactaataa atcgtgaaac aatctcctca tttgttagta ttttcgcctg agcacagcaa
      541 gaggttaata ttgcaaccgt cattaaaaat ggctttatca caaaacaaac tccattgtta
      601 gcatgttacg cgataatttt gcctcatctt tcaccacaaa aaaagaactc accttttaaa
      661 tggagaatac ccaacatcat gggtgatttt cataaaggga tcgcctgttt tcggcaaatc
      721 gataacgcgc attccaggcg gcgcgttgtc aaacgtcacc ttaagttcgc tgcgggttgc
      781 cggtgaagat gcaaggctta acagctgatt cctttccagt gatacattcg gggtgtagcg
      841 tttatcggga gataaatacc catcaccacg tggcatctgc catccggtca tgtcatagac
      901 aaaatcatgg aactgtttgc cccactcgtc gaacttatca ttcagaccaa atccactgtt
      961 cagtgcatca gccacaaaat ttttaacaag ccagggatgt tctttttcaa actcctgcgc
     1021 catcatgccc agttcaagca atccaccaat cagactgatc tttccgaggc cctttaattt
     1081 cagccctgtt tttcctgccg ccgttgtcca ttgattctgg gcgactgttg ccgcacgaat
     1141 acttgccacc gccttcacac caatatacag ggcagaaacc gttgctattg tttttactgc
     1201 ctgttcccat ccccccattg ctctgacaac ctgatcaacc tcctgccaga cttttttcac
     1261 aaccggaccg accgtttccc agttatcaat tatgagatat gcaccaccga cgagaagcgc
     1321 aattaatccc tttgccggcg tcatattcat cacgctaccc atgatttttg ttatgcgtgt
     1381 caatgtgcca atcgccacgc ccatcgtcag taatgccgca ccagttttcg cgatggtttt
     1441 tacgacctca ggattttctc tgacaaacgt gcgcacttct tccagaaaag gcttcatctc
     1501 ttttatgcct tcattgagtg aaggcaggaa ggtttccccc agcgtggaag aaatcgcatt
     1561 gatctggttc tgcagcagca acaactgatt ttctgtcgtc gcggcacggg cggcatattc
     1621 tttctgcatt gagccgccat actgctgcgc atccgccaca cgattaaagt tggttcgcaa
     1681 caaatccaga ttcgtgagca gcggcgcaat ggcccccagg gattccttcc cgaacagcgc
     1741 tttcagcaca gcagcctgtt tttctttcgg cacttttgcc agagaatcca gtacgtgcag
     1801 catggccccg cgggcgtctt tctgcatatc cgccgccagt tttttcgggc taatgcgcaa
     1861 cgagcggagc acttttttct gcgatttggt ggcagaatcc ctcgctgtca gcgacaacat
     1921 aaaattcttt atccccgtcg cagcaatttc tgactccacc cccattccgg caatggttgc
     1981 ccccattgcg gcaatctctc cggaggccac acccgcaaca ctgcctaaag ggccaatacg
     2041 ggtcacaaca tcagaaatct ttttcgcact tgcaggaccg gtattgccaa gatagttaat
     2101 cttatccgca agccctgcca cctctccctg tgtcagttta aaggcagtgc gccactgcgc
     2161 catcatctga ccggattctt ctgccgtggt gtcaaaggcc acgcccatct tcacggcatc
     2221 gtcagtaaac tgcatcagct cgtcacgggc gataccagcc tgaccacccg ccgccacaat
     2281 ttcggcgata ccttccgccg acatgggcag ttctgttgac aggtcgcgca cctgctccgt
     2341 cattgcctta aacgcttccg gcgtatccag accatccacc actttccgga catccgccat
     2401 ttttgattca agggcaatgg cagatttgac cgggagcgcc agcgccccca gtactgcggt
     2461 tccggcaccc gctgcgccca gtgacaggct ggcaaactcc tttttaaaac ctttcagctg
     2521 gcgctgcatt cccttcagcg gtgccgatac cttatccacg gcagtgatga tcgccttcag
     2581 ctgaaaactg tcagccatgc ttcatctcct cgttaatgcg gacggcctcg gcttccagtt
     2641 ctgtaaactg cgaaacagcc acccgtcgca gctccagtgg attcagtttc cagaaccagg
     2701 caacattgta gagtcgtttc cggaggtgct tcccgtctcc gactgggtaa aaaaacgcag
     2761 gatctgcatg ctggtcttaa aaatatccag ttttgccatc tgcgccgccg atgaacacgg
     2821 gatccccgcc agcaacggga tatatttcag tgccacctgg ctgtccagtt taatactgcc
     2881 ttcaccggaa ataatgaaag gaaaacccag cgcctcgatt tcgtcatacg tgggctcgcg
     2941 caactccagc acatgcagca tttcgttatg cgctgtcacc ggttttttta aaactatttc
     3001 tgtaacgttc attgccatta ctgataccct ccctcttcgc cgtggaattc aagatctgcc
     3061 gtgccttctt cggcattatg attagcctct ccgtgcagcc atgccgccga aagcacatac
     3121 accatgccgt ttgccagctc agcggtaatc gtcatctggt ctgaggtggt aattttgtcg
     3181 accggaaagt ttttgggcac tttaaacgtg cccttaacgt aaggtgaccg ccaggtctcc
     3241 ttgtaatcca catcccctgc catgccgacg atgtcatccc ggacattggt gttcatcggc
     3301 acttcaatgc cacctgtcag cgataactgc tgaccatcca ctttaaaaaa acagattccg
     3361 gcgatctttg ccattatgct gactcctctg aatactgaag acggaactgg ttaaccaccg
     3421 caaagacacg taactgatta acataatccg gcgggaacag cgtattcagt cggttcgggt
     3481 tatctgcatc acgctcaact atcagatact gtttaaacag atcgtatttt tccacaatac
     3541 cggcacgctc catctgacga tatgtcgcca gaagttcccc tttgataacg gcaggagtga
     3601 caatcgcctg ccccggacca aaacgggtac catcatttgc cagcttgtga cgtccgtact
     3661 tgcttgtgat gaccgatttc agtttgcgaa gaacatatgc actggtatgc agagtttcac
     3721 tgtccagata gctgttgtct gccacgccat acgcattctt tttgtaggtg gttacggaac
     3781 gctggatctg caacgtgccg ccttccacat aagccgtcgc cacgccgtga gataaaaggg
     3841 tctgctgctc tgtcatgatg aatcgcttac ctttcggtgc cggaagcatc cccaccagct
     3901 cccccgtctg tgtcggacgg gcaggatcat tccggataaa taccgcttca cgggcaaggc
     3961 gactggcaac cagttcatcg acaggcgact gggtttcttt ttcgtaaccg gcaagcgtga
     4021 tatgttgctg attatgcata tctccggcac caaccagctc tgacagcgtt cccagttttg
     4081 cggtatagac atgcccgtat aactggcgcg cataactcca gcgaccgctg ctgtcattca
     4141 tttcggtcat catcatattg atggaggcgg catcgttgaa cggcagaccg ataaagtcga
     4201 atgcctcatc gcccatagca gcaacagcgg cggtaagatc aggcgctccg ctaccggcag
     4261 caccgacttc cgtcaccacc cgaagccctg ctggcaaaat ctcaccacca ccagaaccat
     4321 aataattcag acagaccggt aactcattac catacagccc cttatgacgg gcagtcagcg
     4381 tcaccacgcc tgcatcagat gacgccgtaa acggcagggt gataaccccg tttaccgctt
     4441 ccttaatcgc ggtggcaacc gcagtggcat catcgccatt caccacaggc acctgtacac
     4501 gggagcgccc gacatacaga ctcagggtgc cgctttcctc tgcttctccg gcaaccgtca
     4561 ccctgaccgt cgccgccgcc cctctggctt ccggtactgc aataacatac agttcaccga
     4621 aaggatctgt ctgacggtag acttccacca tacgcgccag ctggctcccc gccccacaaa
     4681 tctggcgggc ataatctgcc gatggcatca gcaccaggct gttaacctca atggctgcat
     4741 cgttgctggc atgcccgatc agtaatgcag gcgcgctggt caccgctgta tttgccgcag
     4801 aattgttcat ctcggcgtag aacaacggca ccagcgtatt cgacggaaca gtattaaaac
     4861 ttatcgtcat gattttttag ccttatcctg tttaacacgt accacatcac ccgccgctat
     4921 acggcggaac cagtagctgc tttcttccac atttcgccct tcggaaggca aaaggtctcc
     4981 acgggcaggg tcaggaaccg accgcccttt caggggtttc acaaacatga gggactctta
     5041 attctgaggg aaacgcattt ccagatggtg ctcaatgtca ccatccgggc cagtaccagg
     5101 atcgatgtaa tccacatcaa tactcagcag ggataaatcc ggcagggcat tcacgtcctc
     5161 tgcctgtcgc gtgtcttctt ccgtgatttc atacttcgcc gtaaaatcaa actggtaata
     5221 cagttcgtaa cggttcagat ccagcagggt gccaccgaca taaacaatct cgcccccttg
     5281 cgggtccggc atccatccaa gcagggcttt ccagagttct ctccggacat catgaacggc
     5341 gtcataggct gcccactgcc ctttttcatc acgc
//
LOCUS       DAZOYF010000220         5339 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZOYF010000220 DAZOYF010000000
VERSION     DAZOYF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.222"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHUOF_003888"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHUOF_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR0612852.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="ACHUOF_003889"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="ACHUOF_003889"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR0612853.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHUOF_003890"
     CDS             1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHUOF_003890"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR0612854.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="ACHUOF_003891"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="ACHUOF_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR0612855.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1457 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DAZOYF010000221         5313 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZOYF010000221 DAZOYF010000000
VERSION     DAZOYF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.223"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(169..930)
                     /gene="kduD"
                     /locus_tag="ACHUOF_003892"
     CDS             complement(169..930)
                     /gene="kduD"
                     /locus_tag="ACHUOF_003892"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR0612856.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(960..1796)
                     /gene="kduI"
                     /locus_tag="ACHUOF_003893"
     CDS             complement(960..1796)
                     /gene="kduI"
                     /locus_tag="ACHUOF_003893"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR0612857.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(2083..3264)
                     /locus_tag="ACHUOF_003894"
     CDS             complement(2083..3264)
                     /locus_tag="ACHUOF_003894"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR0612858.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3519..4748
                     /locus_tag="ACHUOF_003895"
                     /pseudo
     CDS             3519..4748
                     /locus_tag="ACHUOF_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5175..>5313
                     /locus_tag="ACHUOF_003896"
     CDS             5175..>5313
                     /locus_tag="ACHUOF_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0612859.1"
                     /translation="MQLYTDREVLNMGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCA
                     NS"
BASE COUNT         1445 a         1301 c         1121 g         1446 t
ORIGIN      
        1 attattgaga ttattattaa cctcctaatt ttaaagcaga taaaattcat aaagttcatt
       61 aattgataat tgatatggat tatttcataa ccatgatatg gattatgatg atctagagat
      121 ataaaaaacc ctgccatgcg gcagggtcat aaaagtaaga ggaatgaatt aacgcgccag
      181 ccaaccgcca tccacagcaa tggtataacc atttacataa tctgaagcgc tggaggcaag
      241 aaacactacc ggccccatca ggtcactcgg cagtccccaa cggccagctg gaatgcgatc
      301 gagaatttcc gcgctacgtt gttcatctgc ccgcagttgt tgggtattgt tggtcgccat
      361 gtaacccgga gctatcgcat taacattaat gttgtgttta gcccattcgt ttgccatcaa
      421 tcgcgtcaca cccatcacgc cgctttttga tgcggtataa gaaggcacac ggatcccgcc
      481 ctggaaggag agcattgacg cgatattgat aatcttgccg ccattgcctt gcgcgataaa
      541 gtgtttcgcc gctgcctgag acatgaagaa tacgctcttg atattcaggt tcatgacatc
      601 gtcccagtct ttttcgctga actcgagagc gtcttcgcgg cgaatcaatc cggcgttatt
      661 caccaggata tcaatatgac caaattcagc taccgcgcga tccagcagtc caggaatgcc
      721 atcaatcttt cgcagatcgg cggtcaggct taaaaaacga cgccccagcg ccgtgacctg
      781 ctcgatggtt tcagtcggtt caacgatgtt aatgccaaca atgtcacagc ccgcttgcgc
      841 cagccccaac gccatcccct ggcccagccc agtatcacaa ccagtgacga ccgcaacttt
      901 accttggaga gaaaatgcac ttaaaatcat aacaacacct tattcgttta tgcccacaac
      961 tagcgcaaat ctttaacgcc cacatggtcc atatcattaa agacctggtt ttcaccgacc
     1021 atgccccaga taaaggtata agctttggtt ccgacgccgg aatggatcga ccagctcggg
     1081 gagatcaccg cctgctcgtt atgcatcaca atgtgacgcg tttcttgcgg ctgccccatc
     1141 atgtggaaaa cgcaggcgtc atcatccata ttgaaataga aataaacttc catccggcgc
     1201 tcgtgggtgt gacacggcat ggtgttccac aagttacctg gagccagctc cgtcagcccc
     1261 atactcaatt gacaggtttc cagtacatcc gggacaaaat atttgttaat cgtgcgacgg
     1321 ttactggtga ggttatcgcc taacgtgact ggagatactt cgtccggtgt gacttttttg
     1381 gtgggatacg tcgtatgcgc gggtgcgcaa ttgtaataaa acttcgccgg agtggcggta
     1441 tcaatactgg caaagacaac ttcttttgca cctttaccaa catacagggc gtcgcggtga
     1501 ccgatttcat agcattgacc atcgacagta atcgtaccgg caccgccaat attgataaca
     1561 cctaactcgc gacgttcaag gaaatagctt acgccgagtt gtttaccaac ttccccgcca
     1621 acggaaaccg ttttagttat cggcataatg ccgccaacaa taattcggtc aatgtggctg
     1681 taaaccatgg tgtactcatc ggcgacaaat accttttcaa ccaaaaattc attgcgcagc
     1741 ccttgggtat ccagcgtttt tgcgtgcgca ccgtggatgc tctgtcttac gtccacatca
     1801 acctccgata atcagtgaag cgtgaaaata gaaaacgata gtgaaacact gtttcgtttt
     1861 ctctataaag ggatgatatg cccatgggct agggttttca attaaaaata aaacaatgtt
     1921 ttatttttaa tttaaggatt aaaaaaagtg cagtgtcgat cacgaatatg tcatgagaat
     1981 ggaaaaatga gggagcccag aaaatgtatg tcgacttgcc agccatcatt attcaacaga
     2041 ttaatgacgg cagatattta tcggtaagga ggaaatgcag agctactcgt cacgttcaat
     2101 ggtcaatgcc acgccctggc ccccgccgat acaaagcgtt gccagccctt tgcgggcatt
     2161 acgtttcacc atttcatgaa ccagagaaac caggattcgg caaccggaag cgcctatcgg
     2221 gtgaccgagt gcgatcgcgc caccattgac attgacccga cgctcatccc actcaagcat
     2281 cttgccaacc gaaagcgcct gtgcagcaaa cgcttcatta gcctcgataa gatcgacatc
     2341 agccaactgc cagcctacac gctccaggca acggcgggtc gcatacaccg gcgcaattcc
     2401 catcaatgcc ggatctacac caacgctggc aaatgcgcgg atgcgggcca gcacgggtaa
     2461 attcaacgct cgtgctttgg cttcgctcat catcattacc gcagctgcgc catcgtttat
     2521 ggatgatgca ttccccgcgg tcacagaacc gagactatca aatgaaggat ttaaacgggc
     2581 taaaccttct gcactggcgt cagtgcgtgg ctgttcatcg gtatcaacaa ccaacgtctg
     2641 accgttactt tggatcatta ccgggacgat ctcatcttta aatcgtccgg cgtcaatcgc
     2701 cgctcgcgct ttttgttgcg agctaagtgc gtaagcatcc tgcaactgac ggctgatgcc
     2761 atattcgcga gccagatttt cggcggtgac accaatatga taatcattga aggcatccca
     2821 caacccatca tgcaccagac tgtcaaccaa ctggctattg ccaagctgtt caccggtgcg
     2881 gctatcagtc agaacatgtg gtgcgcggct catgttttcc tggccacctg cgatgacaat
     2941 atcagcctcg ccacactgta tcgcctgagt agccagatgc agtgctttaa gaccggaacc
     3001 gcaaacgtca ttaatcgtga ttgcagaaac gctgttagga agaccacctt taatagccga
     3061 ttgccttgcc ggattctgcc ctgcccctgc agtcaacacc tgaccaagaa ttacttcatc
     3121 caccgcatat gcaggaacgc cggtacgttc tattaacgct ttcacgacca gactaccaag
     3181 ttccacggcg gaatgacccg ctaacgcacc acgaaagcag ccgataggtg tccgtaacgc
     3241 cccgacaatc acaacgtctt tcatcacaac ctcgcaccaa ttaacagtgc aatagtaaat
     3301 gattgttaat tacagttatc ttaattgttt aaaaaaagtg atttttatca caaaggaaat
     3361 atgcctgagc agtagtcaga gtcataactg gcacgtaagg tttgcaacca ctaacccacc
     3421 aatagatggg tagatagggc gttaatctcc catacttaac ttggtttatg gtaaattgcc
     3481 ctccattttg tttaatttgt agatgatacg ttcagataat gtctaatatt tggtctaaag
     3541 aagaaactct gtgaagtttc gcgctctatg gcacagccgt tgatgcaggc acgctattcc
     3601 ttcctattca gttaggttcg gcaggggctg tggtcctgtt tattactgct ctggtcgcct
     3661 ggcctttaac atattggcca cataaagcct tatgccagtt catcctctca tcaaaaacat
     3721 cagcaggtga agggataacg ggcgcggtaa cacactacta tggcaagaag attggtaatc
     3781 tgattaccac gctgtacttc atcgcctttt ttgtcgtcgt gttgatatat gcagtggcaa
     3841 ttaccaactc acttacggaa cagctggcaa agcatatggt tattgatctt cgcatccgta
     3901 tgttggtgag tctgggtgtt gtattaattc tgaatctcat ttttctgatg ggacgtcatg
     3961 ccactattcg ggtaatggga tttttggtat tcccattgat tgcctatttc ttatttcttt
     4021 ccatttacct tgtcggtagt tggcaacctg atctattaac aacccaggta gagttcaatc
     4081 agaataccct tcaccagata tggatatcga ttcccgtgat ggttttcgcc tttagccata
     4141 cgcccattat ttctacgttt gccatagaca gacgtgaaaa atatggcgaa cacgctatgg
     4201 ataaatgcaa aaaaattatg aaagtcgctt atctcatcat ctgcataagt gtactgttct
     4261 ttgtctttag ctgcctgctt tctattccat cttcgtatat tgaagcggct aaagaagaag
     4321 gggtcaccat tttatcggcg ctttctatgc tgccgaacgc cccagcatgg ttgtcaattt
     4381 ccgggattat tgtcgcagta gttgcgatgt cgaaatcatt cctgggtacg tactttggcg
     4441 ttattgaagg tgccacagag gtcgtcaaaa caacactaca gcaggttggt gtaaagaaaa
     4501 gtcgtgcatt taaccacgca ctatcaatta tgttggtatc gctgattacc ttcattgttt
     4561 gttgcattaa cccgaacgcg atttcgatga tttacgcgat cagcggcccg ctcattgcca
     4621 tgatactttt catcatgcct acgctgtcaa cgtatctcat cccggcgctt aaaccctggc
     4681 gttccatcgg aaatctgatt acgctgatcg tgggtatcct gtgcgtatcg gtaatgttct
     4741 ttagctaata tccgttgtgg atggttcaaa attgctacaa ttttgaacca ttccattttt
     4801 cataatattc atttgttatt attccgccct atcacttctc tcgtcatatc ctagaattat
     4861 cagttagtta aacacacaaa gagatctgaa catcttaaat aagactattt aagatgcata
     4921 acttagattc gcaagatatc ttctggtcat tcaaaaacaa tttgcgatat agatattctc
     4981 ataccatgag atagttatct ggaccacgtt cgaccatgca agattaaatc agtgaaacat
     5041 aatcatattt aataacgaga aatgcatttt taaatatctt aatcagaatt atagcaaata
     5101 cagattaatc gcaatttttc ccagcaaaat tacaaaatca aggtgtattg acatcatcaa
     5161 tcaccaatga aattatgcaa ttatatacgg atagggaggt tcttaacatg gggctttgta
     5221 gtcgttataa aagtcttaca tgcaatagtt gctcaatgca ttgccaaata atgccagaag
     5281 agtcaccgcg tttgcagtac tgcgcaaact cgt
//
LOCUS       DAZOYF010000222         5280 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZOYF010000222 DAZOYF010000000
VERSION     DAZOYF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.224"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(67..1827)
                     /gene="ycaO"
                     /locus_tag="ACHUOF_003897"
     CDS             complement(67..1827)
                     /gene="ycaO"
                     /locus_tag="ACHUOF_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR0612860.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1957..2583
                     /locus_tag="ACHUOF_003898"
                     /pseudo
     CDS             1957..2583
                     /locus_tag="ACHUOF_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            2782..3870
                     /gene="serC"
                     /locus_tag="ACHUOF_003899"
     CDS             2782..3870
                     /gene="serC"
                     /locus_tag="ACHUOF_003899"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR0612861.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            3941..5224
                     /gene="aroA"
                     /locus_tag="ACHUOF_003900"
     CDS             3941..5224
                     /gene="aroA"
                     /locus_tag="ACHUOF_003900"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR0612862.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         1313 a         1263 c         1358 g         1346 t
ORIGIN      
        1 taaaaaaacg ccccgtaata taactcagac taatcattaa gcctacattg cgtaggctat
       61 ttgattttat tttgcccaga atgctgcttt ggcgcgctgc agcttttcgt aggccttcaa
      121 caacgactga tgtgcagcaa acgcgtgcag atcgctatct accggttgca ggccgtaaaa
      181 cgccgcttcg ccgctcattg ccgcactggc ggcttctacg gcatctgcgc cgtacatgcg
      241 aacaaacgca ttcagatatt gcagcggctg gcgatcttct tcctgtgcca gtaataacaa
      301 cgtttgcagg cagcgataat agttggcgcg ttccggacta aatactgatg agttaaactc
      361 catcgtccat tcggtccaga ccagagcctg ttccagatcg ccaccagcca gcgccagcat
      421 ggcttttaat tcaccgatac gcagggtgta ccaaccgtta tccgacccgg tcgccagacc
      481 caacagctca cgcacgcggg taaagtcatc aaaaccttct tcatccagtt gctcgatgag
      541 gttcaggtaa tcttcttttt cccactcgct gcctggtagc gaaagaatcg tttcacgtaa
      601 atggctgccc atactgttat tcgcgagcca cagatcttca gccggataaa tatcggacat
      661 gccaggcacg ataatacggc aagcataaac gcccagatgc tcgtaatcgg caatataaac
      721 ttctttatct tctttgttga agatagccat cagcgtagca aactcttctt ccgtggtgcc
      781 ggagaaattc cagtccacaa acggataatc ggcatcctgc ttgaacaggt cccaggagat
      841 taaaccgctg gaatcgataa agtgcgtttc gaggttggta tgttcagcga cttcttcatc
      901 atcgaaggtt ggcggagtaa acacatccaa atctttcagg ccacgacctt gcagcagctc
      961 ggtcacggta cgttccagtg ctacgccaaa atcaggatgc gcaccgaaag aggcaaagca
     1021 ggtgccgttt gcaggattaa acagtaccac gcaaatcacc ggatactggc cgccaaggga
     1081 accatcatat gcgaagatcg ggaaaccttc tgtttccagt gtttcgatcg cttcaaccac
     1141 cgctgggtaa cgcgccagca cgtctgccgg gatttccggc aagctgatgc tttcagcaat
     1201 aatgcggttt ttcacgtagc gttcgaaaac ttcggacaac ccctgaacgc gtgcttcgtt
     1261 gcgggtattg ccagcggaca taccgttaga aacgtacagg ttaccaatga tattcatcgg
     1321 aatataaacg gtctgattgt cggactggcg cgtaaacggc agaccgcaaa taccacgatc
     1381 ttcgttaccg gattgtaggt caatcagcat actgccggtc aattcattct ccggatcgta
     1441 aaatgcgcgc agacggtcat cgagcagccc ttctggcaca tcgtcatttt cgctcagtgg
     1501 gaaccatttt tcgttgggat aatgcacaaa tggaccgttg gcgatggttt cgcccagcca
     1561 gaaatcagca aaaaagtagt tggttgagag acgctcgaaa tattcaccga gtgcagaagc
     1621 cagcgccgct ttcttggttg cgcctttacc gttggtaaaa cacagtgcgc actctttgtc
     1681 gcgaatatgt acagaccaga cgttaggcac gggattcagc caggaggcct cttcaatctg
     1741 aaagccgagg tctgaaagtt tttgctggaa gcgagcgatg gaatcttcca gagcggcatc
     1801 tttgccgggg ataaatgttt gcgtcatgaa aatcacttta gtcgtacgga aagcgcgcaa
     1861 taatacgggt tttatctcaa aggcgctatc accgccgcca tgccgatgaa ctgttgacta
     1921 tgcttttagc ggataaacca cgttaagagc ataaaaatga aggcgttcga tctccaccgt
     1981 atggcatttg ataaagtgcc ttttgatttc cttggcgaag ttgcactacg tagtctttat
     2041 acctttgtac tggttttttg ttcctcaaaa tgaccggaag acgcggtgtg cgacagatgt
     2101 cgctgtttga agtgttaatc attctcacgc tgggttcagc ggcgggagat gtggcgtttt
     2161 atgatgatgt gccgatggtg ccggtactta tcgtctttat cactctggcg ttgttatacc
     2221 gcctggtaat gtgatggcga gctggcctgg tcgaaactca ataactccaa catgacggaa
     2281 tttgagttct ttatggagct acgattgcgt ggcgtggagc agctggggca ggtacgtctg
     2341 gcgattctcg aaaccaacgg gcaaatcagt gtctatttct ttgaagatga caaggtgaaa
     2401 ccgggtttac ttattttacc cagtgattgt acgcagcgtt acaaagtggt gccggagtcg
     2461 gcggactatg cctgcattcg ttgtagtgaa atcattcata tgaacgcggg ggaaaaacaa
     2521 ttatgtccgc gctgtgcaaa tccagaatgg acgaaggcaa gtcgggcaaa acgggtgacc
     2581 tgacagtaaa aacatcggct ttttgctaat aatccgagag attcttttgt gtgatgcaag
     2641 ccacattttt gccctcaacg gttttactca ttgcgatgtg tgtcactgaa tgataaaacc
     2701 gatagccaca ggaataatgt attacctgtg gtcgcaatcg attgaccgcg ggttaatagc
     2761 aacgcaacgt ggtgagggga aatggctcaa atcttcaatt ttagttctgg tccggcaatg
     2821 ctaccggcag aggtgcttaa acaggctcaa caggaactgc gcgactggaa cggtcttggt
     2881 acgtcggtga tggaagtgag tcaccgtggc aaagagttca ttcaggttgc agaggaagcc
     2941 gagaaggatt ttcgcgatct tcttaatgtc ccctccaact acaaggtatt attctgccat
     3001 ggcggtggtc gcggtcagtt tgctgcggta ccgctgaata ttctcggtga taaaaccacc
     3061 gcagattatg ttgatgccgg ttactgggcg gcaagtgcca ttaaagaagc gaaaaaatac
     3121 tgcacgccta atgtctttga cgccaaagtg actgttgatg gtctgcgcgc ggttaagcca
     3181 atgcgtgaat ggcaactctc tgataatgcg gcttatatgc attactgccc gaatgaaacc
     3241 atcgacggta tcgccatcga cgagacgcca gacttcggca aagatgtggt ggtcgccgcc
     3301 gacttctctt caaccattct ttcccgtccg attgacgtca gccgttatgg tgtaatttac
     3361 gctggcgcgc agaaaaatat cggcccggct ggcctgacaa tcgtcatcgt tcgtgaagat
     3421 ttgctgggca aagcgaatat cgcgtgtccg tcgattctgg attattccat cctcaacgat
     3481 aacggctcca tgtttaacac gccgccgaca tttgcctggt atctatctgg tctggtcttt
     3541 aaatggctga aagcgaacgg cggtgtagct gaaatggata aaatcaatca gcaaaaagca
     3601 gaactgctat atggggtgat tgataacagc gatttctacc gcaatgacgt ggcgaaagct
     3661 aaccgttcgc ggatgaacgt gccgttccag ttggcggaca gtgcgcttga caaattgttc
     3721 cttgaagagt cttttgctgc aggccttcat gcactgaaag gacaccgtgt ggtcggcgga
     3781 atgcgcgcgt ctatttataa tgccatgccg ctggaaggcg ttaaagcgct gacagacttc
     3841 atggttgagt tcgaacgccg tcacggttaa tgccgaaatt ttgcttaatc cccacagcca
     3901 gcctgtgggg tttttatttc tgttgtagag agttgagttc atggaatccc tgacgttaca
     3961 acccatcgct cgtgtcgatg gcactattaa tctgcccggt tccaagagcg tttctaaccg
     4021 cgctttattg cttgcggcat tagcacacgg caaaacagta ttaaccaatc tgctggatag
     4081 cgacgacgtg cgccatatgc tgaatgcatt aacagcgtta gggttaagct atacgctttc
     4141 agccgatcgt acgcgttgcg aaattatcgg taacggcggt ccattacacg cagaaggtgc
     4201 cctggagttg ttcctcggta acgccggaac ggcaatgcgt ccgctggcgg cagctctttg
     4261 tctggatagc aatgatattg tgctgaccgg tgagccgcgt atgaaagagc gcccgattgg
     4321 tcatctggtg gatgcgctgc gcctgggcgg ggcgaagatc acttacctgg aacaagaaaa
     4381 ttatccgccg ttgcgtttac agggcggctt tactggcggc aacgttgacg ttgatggctc
     4441 cgtttccagc caattcctca ccgcactgtt aatgactgcg cctcttgcgc cggaagatac
     4501 ggtgattcgt attaaaggcg atctggtttc taaaccttat atcgacatca cactcaatct
     4561 gatgaagacg tttggtgttg aaattgaaaa tcagcactat caacaatttg tcgtaaaagg
     4621 cgggcagtct tatcagtctc cgggtactta tttggtcgaa ggcgatgcat cttcggcttc
     4681 ttacttcctg gcagcagcag caatcaaagg cggcactgta aaagtgaccg gtattggacg
     4741 taacagtatg cagggtgata ttcgctttgc tgatgtgctg gaaaaaatgg gcgcgaccat
     4801 ttgctggggc gatgattata tttcctgcac gcgtggtgaa ctgaacgcta ttgatatgga
     4861 tatgaaccat attcctgatg cggcgatgac cattgccacg gcggcgttat ttgcaaaagg
     4921 caccaccacg ctgcgcaata tctataactg gcgtgttaaa gagaccgatc gcctgtttgc
     4981 gatggcaaca gaactgcgta aagtcggcgc ggaagtggaa gaggggcacg attacattcg
     5041 tatcactcct ccggaaaaac tgaactttgc cgagatcgcg acatacaatg atcaccggat
     5101 ggcgatgtgt ttctcgctgg tggcgttgtc agatacacca gtgacgattc ttgatcccaa
     5161 atgcacggcc aaaacatttc cggattattt cgagcagctg gcgcggatta gccaggcagc
     5221 ctgaatgaac aacgggcaat aaatagccaa atctttcttt atcaaaacgt cggcacattg
//
LOCUS       DAZOYF010000223         5250 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZOYF010000223 DAZOYF010000000
VERSION     DAZOYF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.225"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..171
                     /locus_tag="ACHUOF_003901"
     CDS             <1..171
                     /locus_tag="ACHUOF_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0612863.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="ACHUOF_003902"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="ACHUOF_003902"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR0612864.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="ACHUOF_003903"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="ACHUOF_003903"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR0612865.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHUOF_003904"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHUOF_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR0612866.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="ACHUOF_003905"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="ACHUOF_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR0612867.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1291 a         1181 c         1387 g         1391 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc
//
LOCUS       DAZOYF010000224         5183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZOYF010000224 DAZOYF010000000
VERSION     DAZOYF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.226"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..1154
                     /locus_tag="ACHUOF_003906"
     CDS             <1..1154
                     /locus_tag="ACHUOF_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612868.1"
                     /translation="EDMCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKY
                     SAKDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNV
                     PKNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACE
                     AHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFN
                     QSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVM
                     GCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKD
                     LFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="ACHUOF_003907"
     CDS             1833..2051
                     /locus_tag="ACHUOF_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR0612869.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2269..2511
                     /gene="yiiF"
                     /locus_tag="ACHUOF_003908"
     CDS             2269..2511
                     /gene="yiiF"
                     /locus_tag="ACHUOF_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR0612870.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="ACHUOF_003909"
     CDS             complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="ACHUOF_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR0612871.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="ACHUOF_003910"
     CDS             complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="ACHUOF_003910"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0612872.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4399..>5183)
                     /gene="fdoH"
                     /locus_tag="ACHUOF_003911"
     CDS             complement(4399..>5183)
                     /gene="fdoH"
                     /locus_tag="ACHUOF_003911"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR0612873.1"
                     /translation="GCKACQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSE
                     VEQNDKLEWLIRKDGCMHCSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAG
                     CPFDIPRLNPEDNRVYKCTLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERV
                     AELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWK
                     PLAAVGFAATFAASIFHYVGVGPNRADEEENNLHEEKDEERK"
BASE COUNT         1404 a         1203 c         1265 g         1311 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     2101 attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg
     2161 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa
     2221 gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg
     2281 gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt
     2341 gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat
     2401 caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg
     2461 tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa
     2521 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
     2581 ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga
     2641 taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc
     2701 gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa
     2761 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc
     2821 cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa
     2881 ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct
     2941 gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag
     3001 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
     3061 ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac
     3121 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
     3181 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     3241 ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc
     3301 gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc
     3361 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     3421 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     3481 agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc
     3541 agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca
     3601 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     3661 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     3721 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     3781 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     3841 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     3901 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     3961 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     4021 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     4081 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     4141 aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga
     4201 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     4261 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     4321 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     4381 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     4441 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt
     4501 agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     4561 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     4621 cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta
     4681 accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt
     4741 gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     4801 cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     4861 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     4921 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca
     4981 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     5041 ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt
     5101 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     5161 cacctgacag gctttacagc cga
//
LOCUS       DAZOYF010000225         5171 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZOYF010000225 DAZOYF010000000
VERSION     DAZOYF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.227"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..244
                     /locus_tag="ACHUOF_003912"
     CDS             <1..244
                     /locus_tag="ACHUOF_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612874.1"
                     /translation="EMLSFLTSEEREALTNMLARKGVETVLRLLDKETQELLQLEGRK
                     RLAALMLVDMPDEQVREILEEIEASILSESVNAKAG"
     gene            493..1437
                     /gene="tauA"
                     /locus_tag="ACHUOF_003913"
                     /pseudo
     CDS             493..1437
                     /gene="tauA"
                     /locus_tag="ACHUOF_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="ACHUOF_003914"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="ACHUOF_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR0612875.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="ACHUOF_003915"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="ACHUOF_003915"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR0612876.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="ACHUOF_003916"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="ACHUOF_003916"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR0612877.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHUOF_003917"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHUOF_003917"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR0612878.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1181 a         1305 c         1472 g         1213 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaatatt tttaatcttt atttatagct cttggttatt
     5161 attttttggc g
//
LOCUS       DAZOYF010000226         5165 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZOYF010000226 DAZOYF010000000
VERSION     DAZOYF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.228"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..222
                     /locus_tag="ACHUOF_003918"
     CDS             <1..222
                     /locus_tag="ACHUOF_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0612879.1"
                     /translation="SLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFH
                     WGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(394..723)
                     /locus_tag="ACHUOF_003919"
     CDS             complement(394..723)
                     /locus_tag="ACHUOF_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR0612880.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(728..913)
                     /locus_tag="ACHUOF_003920"
     CDS             complement(728..913)
                     /locus_tag="ACHUOF_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR0612881.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(910..3549)
                     /locus_tag="ACHUOF_003921"
     CDS             complement(910..3549)
                     /locus_tag="ACHUOF_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR0612882.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3757..4746
                     /gene="ldhA"
                     /locus_tag="ACHUOF_003922"
     CDS             3757..4746
                     /gene="ldhA"
                     /locus_tag="ACHUOF_003922"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0612883.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4857..>5165
                     /gene="hslJ"
                     /locus_tag="ACHUOF_003923"
     CDS             4857..>5165
                     /gene="hslJ"
                     /locus_tag="ACHUOF_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR0612884.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1308 a         1323 c         1204 g         1330 t
ORIGIN      
        1 agtttgtacc gaatgtttgc cgataaaggt ttggtagtcg cgcaatatat tcgtaaccgt
       61 cgtctcgatt tttgtgcaga tgcgattcgc catgccgcag atgatgaaaa actggcaggc
      121 atcggctttc attggggatt ttctgaccag agtcattttt caacggtatt taagcaacgc
      181 tttgggatga cgccaggcga gtatcgacgt aaattccgct aactaattgg cgttgcatgt
      241 actgcaacgc caattagtta aaacagatat ttgactatgc atttttcagg gcgaaaggca
      301 atttgacagg agtttttgaa tattcacctt gtttcaggaa aatagatcaa tcgctttctc
      361 ctgtagcgtt atcccgatat tgcggttttg ctattaaaac tttcgcaccc atttaccgtt
      421 tatcccttgt acatattccc ccggtttggc ccgagctacg agcttttgcc cggcgagttt
      481 cgcgatatca tctacagaca cattattttg ctttgcccgt tgttggtaac tcgctttacg
      541 ttcggcatta atttcggtta ctaatttctc tgtttcagca tccgttttca gcgcaaccag
      601 ataaccataa aatgtttcac ctacccgccc ctgagttctg gcttcatcca gagtcaatgc
      661 cattacattg ctgcttacca gcccaacaag aaacgcacaa agaagtaatg ttttcttcat
      721 catcgcctca gaaaagatcg ctacgagttt caagcagctc ttcgacatct ttgtctgcct
      781 tgatgatgat ctcatgctca attttaacgt tcatattgat agtgatcggt tccttaggtg
      841 cagcgacttc aatgcgaggc gtacagccaa ccagcataaa agatgacgtc aacgcagcca
      901 gtaaaatttt cattgttttt cctcacactc ttttccttgc ggacagtcat ttcccggcag
      961 acgtgcgttc tgctccaacc atgcctggag attatcgcca aagcgtaaac tgcgccacag
     1021 cgtaaaaata ttctcttcat gatggtaatt aaggtttacc gtaccgcttt taccatcaac
     1081 gcgactggta cctgtaatgt tggcctgcat ggttaataaa ccgagattat ctaagttaat
     1141 ttttgtcgat gaccggctaa tttccatata gcgcaaccag ttaattgctg aacccgcagt
     1201 catattgtct ttcaccaccg catctgcggt atctttatcc agccgcaagg tcattagccc
     1261 gctatttgcc agccaaccat ctttcactat ccatttttcg ttgtttagcc ataacggcaa
     1321 tgcaccacta aatgccccgg acatggcgaa ttgtttcgga ttgacggcgc taaccagttc
     1381 gctggatgag agattattca gccgcaacag cgccgggtca tgttgcggca tacgtaattg
     1441 tttcatcagc acgttaccgc ctaacacatc gacgctaaca tcagtcaaca gcaagggttc
     1501 ttcttcagtc cacggataac gcccttgcaa atcagccgta atatttttcg ctgtcaccag
     1561 attaatcact tcggcaattc gcaacgtaac ggggccgcga gtccccagat gccaggctcc
     1621 atcggcaaaa cggaaaggca ggacaaaatc gacaccgtta acctgattat ctggcatcca
     1681 ggcactaccg cctttcaaca cgccgtgccc tcccgcgcgg aatccttgtt caggcgcagc
     1741 agaaaatgca acctgagcat acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg
     1801 caccagcggc tggaatacag tcagtgattg ttttggccac caggcgttac cgcgcagacg
     1861 aataccgtcc cagcgaccat ttacccgaac cgggccaatc tcaccagcat gtaaatcgcc
     1921 tttaaagagg aaataggtcg gattgcgccc atcgacgcta aattttaagg ttgatggcgg
     1981 taacacactg ccgccagtga acagcgtttg cccggcgtcc agtgacagcg cgccgctgaa
     2041 ggagggatgc tgtgcgtcac gtacccagac gacgggcttg tcgagaatta atcgcggctt
     2101 ttctaccgtc atcgtaccgt attgtaactg gtcgaaaccg gtggagagat cggtcagcgt
     2161 aatcgtgctg tcatgccact caccttttcc tgcgacatcc caggtggcat tcatcggtgt
     2221 aaaactccct tttccccagt agcgccactg ccagcggcca gcgtcaggga gaaaatcatt
     2281 cgccagccca tccatatgca gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat
     2341 ggcctgcaaa cgtccgtcaa caccacgttg ggtgactttt acacccgcta aaggccagcg
     2401 gatttcatcg atgtccagcg aatcgatgac tcttcccttt gaacgaagta acgcgcctgg
     2461 ctcaaaggtc agcgttgggt cagacagact tccacttagc tgcgcaggta aacgggcata
     2521 taaaatgagg tccgcctgtt tcgcttcacc ggtcagctgc agaggcagtt gactgttatc
     2581 catgcttaat tttcctgggc caaaattaag caccgcgttg cccttacccg cttgcccctg
     2641 ggtcagcaca ttcagtcgtc cgctgaccag agcgttctca agccctgcct gccagttgtc
     2701 gactttgaca cccagtcggc cactcaaagg aaaaccagca tacagccagc tccagcgacc
     2761 atcgcttacg gtcaattgtt gacgagtaat ttgccacggc aaatcgagca acggatcgcc
     2821 gttatcccgt gccagcacga tcaattgccc gctattttcc tgccagtcca gctcggcatc
     2881 caccagtgac ggttcctgcg gcaagtttaa cgtcgcagta gcatgcccac ttacaggaag
     2941 tccatccggc acgagcggca tagtaaattc ccccaccagt ttaaccggcg gctgattttc
     3001 aaacgcgacg acatccagtt cgctgactgt aagttgttgc cctttcagct ggccttgaaa
     3061 tttaactttt tcgccctgat aacgcagttg ctggatatca gaggttaatg cgagagagag
     3121 ttttccctgc cattcctgcc agggagaaaa aatcagttta tcgatattga tccaggtgtt
     3181 aggcagcatg gactgccact gcgcgagggt ttttggagca gctggcgatt gctccgtctg
     3241 cggcaatttc gccagacaag cagaatcaag ttctaccgtg ccgacgttca ataaccagcg
     3301 gctgggatgt gaaaggctgg cgttggtgat atgcgcaagc tgacaatctc ccaccagata
     3361 acggagatcg ggaatgatta aacctttacg cgtaatgcgt gggctttcat ctaatgcaat
     3421 acgtgtcccg agcggtagcc agatgtccgc cagcgtggga acccacagcc cgagcgtcat
     3481 cagcagcgtc aacggcacaa gaataatcag taataacagc gcgagaacgg ctttatattt
     3541 acccagcatg ggtagttaat atcctgattt agcgaaaaat taagcattca atacgggtat
     3601 tgtggcatgt ttaaccgttc agttgaaggt tgcgcctaca ctaagcatag ttgttgatga
     3661 atttttcaat atcgccatgg ctttcaatta tatttgaaat tttgtaaaat atttttagta
     3721 gcttaaatgt gattcaacat cactggagaa agtcttatga aactcgccgt ttatagcaca
     3781 aaacagtacg acaagaagta cctgcaacag gtgaacgagt cctttggctt tgagctggaa
     3841 ttttttgact ttctgctgac ggaaaaaacc gctaaaactg ccaatggctg cgaagcggta
     3901 tgtattttcg taaacgatga cggcagccac ccggtgctgg aagagctgaa aaagcacggc
     3961 gttaaatata tcgccctgcg ctgtgccggt ttcaataacg tcgaccttga tgcggcaaaa
     4021 gaactggggc tgaaagtagt ccgtgttcca gcctatgatc cagaggccgt tgctgaacac
     4081 gccatcggta tgatgatgac gctgaaccgc cgtattcacc gcgcgtatca gcgtacccgt
     4141 gacgctaact tctctctgga aggtctgacc ggctttacta tgtatggcaa aacggcaggc
     4201 gttatcggta ccggtaaaat cggtgtggcg atgctgcgca ttctgaaagg ttttggtatg
     4261 cgtctgctgg cgttcgatcc gtatccaagt gcagcggcgc tggaactcgg tgtggagtat
     4321 gtcgatctgc caaccctgtt ctctgaatca gacgttatct ctctgcactg cccgctgaca
     4381 ccggaaaact accatctgtt gaacgaagcc gccttcgatc agatgaaaaa tggcgtgatg
     4441 atcgtcaata ccagtcgcgg tgcattgatt gattctcagg cggcaattga agcgctgaaa
     4501 aatcagaaaa ttggttcgtt gggtatggac gtctatgaga acgaacgcga tctgttcttt
     4561 gaagataaat ccaacgacgt gatccaggat gacgtattcc gtcgcctgtc tgcctgccac
     4621 aacgtgctgt ttaccgggca ccaggcattc ctgacagcag aagctctgac cagtatttct
     4681 cagactacgc tgcaaaactt aagcaatctg gaaaaaggtg aaacctgccc gaacgaactg
     4741 gtttaatctt gcagctcccc tgcaatccag gggagctgat tcagataatc cccaatgacc
     4801 tttcattctc tattcttaaa atagtcctga gtcagaaact gtaattgaga accacaatga
     4861 agaaagtagc cgcgtttgtt gcgctaagcc tgctgatggc gggatgtgta agtaatgaca
     4921 aaattgctgt tacgccagaa cagctacagc atcatcgctt tgtgctggaa agcgtaaacg
     4981 gtaagcccgt gaccagcgat aaaaatccgc cagaaatcag ctttggtgaa aaaatgatga
     5041 tttccggcag catgtgtaac cgctttagcg gtgaaggcaa actgtctaat ggtgaactga
     5101 cagccaaagg gctggcaatg acccgtatga tgtgcgctaa cccgcagctt aatgaactcg
     5161 ataac
//
LOCUS       DAZOYF010000227         5150 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZOYF010000227 DAZOYF010000000
VERSION     DAZOYF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.229"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            189..446
                     /locus_tag="ACHUOF_003924"
                     /pseudo
     CDS             189..446
                     /locus_tag="ACHUOF_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHUOF_003925"
     CDS             complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHUOF_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR0612885.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHUOF_003926"
     CDS             complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHUOF_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR0612886.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHUOF_003927"
     CDS             complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHUOF_003927"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR0612887.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3071..3214)
                     /locus_tag="ACHUOF_003928"
                     /pseudo
     CDS             complement(3071..3214)
                     /locus_tag="ACHUOF_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3214..3430
                     /locus_tag="ACHUOF_003929"
                     /pseudo
     CDS             3214..3430
                     /locus_tag="ACHUOF_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3604..4419)
                     /locus_tag="ACHUOF_003930"
     CDS             complement(3604..4419)
                     /locus_tag="ACHUOF_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0612888.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4516..>5150
                     /locus_tag="ACHUOF_003931"
     CDS             4516..>5150
                     /locus_tag="ACHUOF_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0612889.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1375 a         1175 c         1225 g         1375 t
ORIGIN      
        1 ctattttacc gcgacaattc attcagaaca tcaatagtca gggaaggaag tagcaacatt
       61 agctaactaa tcaatgcgtt gattgtaaat ccagctaaga ggtgaggttt tcagagcaga
      121 caacggtgaa acgtcatggt attgttacgt ttaggtaaca agaaatttgt ctgcacaagg
      181 attacatcat gattatggcg aaactgaagt cagcgaaagg gaagaaattt ctctttggtt
      241 tgttggcggt tttcattatt gcggcgtcgg ttgtgactcg cgcgaccatc ggcggcgtta
      301 tagaacagta caatattccg ctgtctgagt ggacgacatc aatgtatgtg atttagtcat
      361 cgatgatttt tgtttatagc ctggtcttta ctgtgttgct ggcaatcccg ttgggaattt
      421 atttccttgg cggcgaagag cagtaagtaa aaaataggcc cgataactcg ggccttgtca
      481 gttattgaag agtcgttaat cgtcttcttc gtcatccagt tcaacgggtg tctgatactg
      541 gtcaggttta atgaccagca ggtcgcagcg aagatgatca atcacctgtt ccgccgtgtt
      601 gccgaggaat gctgctgaaa taccggtgcg tccgaccgtg cccagaacca caattcccgc
      661 ctgtaaatgc tccgccaaat caggaatcac ctcttcgggc agaccttttt ctacgtgcgt
      721 catgttttca ttaatgccga atttctgccg cagggctttc attgccagca aatgttgccc
      781 acgaatggca tcgttataaa cgctcgggtc aaattccggc agttcaatcg cgatattaat
      841 tggcgttacc ggataagcgc caaccagatg aacttcggta tggttgactt gttctgccag
      901 ttcgatcgtc tctttgacca gtttttcatt gagcgcatta tgatacggct cttcactggc
      961 gagattcacc gccaccagcg ccttgcctcc ttccggccac ggctggtctt tcaccatcca
     1021 caccgggctt gggcatttgc gtaacagatg ccagtccgtt ggcgtaaaaa tcaccgcttc
     1081 cagacggtca tgttggtgcg ccatttttag caccaaatcg tgtccgccgc tgatcacttc
     1141 ctgaatgatg gcttcgaaag gacggttatg ccagaccact ttaatttcaa tgggaacgcc
     1201 agcattgaga taatattttg cctgctcgtg gatccaggct gtacgctggc tgatgacgcc
     1261 ctgacgcata gcggtacgtt cgtccgggga gagcagggtg gtcatttcgt atgagaagtc
     1321 atagatcggc aaaaaggctt taattttgcc accaatccgt tgatgtaaat aaacagctcg
     1381 ccgcaatgct ggttggtcgt cctggttagg atcgataaca acgagcatgt tctgatacat
     1441 agccatacag ggtctcctta caacaactgt caacgcagtt tgtaattaaa agattaaccc
     1501 atatctggtg aatgaaacag tgatgaacct tctgccagat caataaatca gaaaaattta
     1561 atgatatgac agaaggatag tgagttatgc ggaaaaatca ggcaacgtta cgcgtatggc
     1621 cagcaagctg ggccagcgca tcgttatttt cgatggtgat gtatttacct ttgactgcca
     1681 gcataccgct tttctggaag cgacccagca gacggctgat ggtttctacc gtcaggccca
     1741 gatagttacc aatatcgcca cgagtcatcg tcaggcggaa ttcacgaggg gagaagccgc
     1801 gttgggcaaa acgacgggac aggttgtaga tgaatgcagc cagacgttcc tcggcatttt
     1861 tcttcgacaa cagcaagatc atgtcctgat cgcctttgat ttcaccgctc atcagacgca
     1921 tcatctgctg acgcagattc ggcattttac cggacaaatc gtccagcgtt tcgaacggaa
     1981 tttcacatac catcgaggtt tccagcgcct gagcgaagct cgggtgatgg ccgctgccga
     2041 tggcgtcaaa tcccactagg tcgcctgcta aatggaaacc agtgatttgc tcgtcgcctt
     2101 gctcagtgat tgtataactt ttaatcgtac cggagcggat ggcataaagc gatttaagtt
     2161 catcaccagc cttaaacagc gtctggcctt tctgaatagg cttcttccgc tcaatgatat
     2221 tatcaagctg atcaagctca tgttcgttga gtgtgaacgg gatgcaaagc tggctgatgc
     2281 tgcaatcctg gcaatggata gcacaaccgc cagactgaat gcgccgtata attcgctttt
     2341 ccgggatcat aggtctgctc aagccgtaat tgatatttgt caattttaac atctttttag
     2401 ggagcaagta agtctaagca aaccttaaca gcagagaatt ccgatattag atgtaaatat
     2461 atgtctatct atttgaaact ccttaagttg tcaagggtaa ctttacataa aagtgtgaac
     2521 aagctggcac aaattgttta atgtttacag caaaagataa ccttcgtggc gcaataacca
     2581 ctcttttcgc tgaactccgc ctgcatatcc ggtcatggtg ccgtttcggc caataacccg
     2641 atggcaaggt acgacgatgc tgatgggatt cgatccgttt gccgcaccaa cggcacgcgc
     2701 cgcgccaggg cggcccaatt gttcagccag ttggccgtaa tgcattacct gcccgcaggg
     2761 gatagtgcgt agtgttttcc agacttcgcg ctgaaatggc gtcccccccg tggcagtggg
     2821 aagcgtatca ataatgctaa gattaccggc aaaatattca cgaagcttgt cgcttaaacc
     2881 acctgggttg gtggcagaaa tgcgctcata gccttctttg cgataatgga tgtccagcag
     2941 ctgcaccatg cgttcgctgt actcttccca ttcaaccgcc cgcaggcgaa attgctcatc
     3001 gcaaatcacc cacagtggac ccagtggcgt ggcaattttt tcttcaagta atctcagcat
     3061 ccgttctctc ttaagacaaa cgtgggtaaa taccctgacc tatcggcaga ccgacaagat
     3121 accacgccag caacatcagc agccatacca ccaaaaagat aagtggatag ggcaagacta
     3181 acgaatagta agtacccagt ttcgcgtctg gtttgtcgca gtctgatagc ataaagcgat
     3241 tttttaattt ttctgcctga agaaatgagg gcaagccatc acatggatcg agggtgggat
     3301 aattccagtc agtgaaattt taccgtaggc tatctgttat ttgattctgt ggcgtgatag
     3361 ccgccagaca ccactgaact cgtcactcat tccccaatcc cggcgagaat gtggttattt
     3421 acagagctaa aaaatgaata aaaaaataga gcctgtcgac atccgccaga ctctacagta
     3481 cacacagcag tgcatcctcg tcttaaatcc ggcctggggt cctggtgacc ggtgacagtg
     3541 ttataacggt tttacccatc cgttgccaat tttcttcagt gacagatttc acgaaaatat
     3601 aagttaaatc atttgtcttg tgattgcata attatgcatc gttaatcgac gagtaaattt
     3661 accgggcgag tagcccgttc gacgcttaaa catattgccg aacgattcag ctgaggcata
     3721 gcccagacga tgggcaattt tggtcaacga atatccatca tcaagaagcg tcactgcaga
     3781 aataacgtgc atttgctgaa cccagtggcg aaatgttaat cccgtctctt taacaaataa
     3841 gcgcatgagt gtacggctac tgacgttaat tttatccgct agattatcca gtgttggcag
     3901 atacccttgg ttgtttagca aattctcaca aagctgctgt agccgctcat catttggcca
     3961 agggagttca agtgacattt tcacccccat ttttatttcc tgaagtatca gtgtctcgag
     4021 ggctgaaaaa cgtaactgtt gtttatctgt ggtactttgc gacggtggaa gttggttaat
     4081 tgcaattagt aattccctta acaggggtga aatacccgtc atgaagcaaa ctgttcccag
     4141 cgtttcactt tccgcttctg aaaccagtac ggtttttaaa agtacgttgc ctgtcgcaac
     4201 aaacttatgc ggcatttgtg ggggcagcca aagcgcgctg ctggcatgga ctagccagac
     4261 gctttctcca gcccatacac gaacgactcc ttcagccggg taaagcaact gggcatagag
     4321 atgtttatgc cagggaactg tacctccatt cagatgttcc gaatccagtc cgataatgtg
     4381 atgccctggt gatgctttgg gttgcagtgt aattgtcatt tacagtaggt attttgtcag
     4441 aaatcatcaa ataagccatg ttaaatttta aatatgacgt atgcaagttt ccggatatat
     4501 acggtgtaaa gagtgatgat aatttctaaa aaacagctta ttggggtggt agcgatcggt
     4561 attcttttga ctggcgttgt cttttttatc tggagggtaa gcaaaggaag atttatacag
     4621 acgacggatg atgcttacat tggtggtaat atcaccacgg tggcaagtaa agtttcaggt
     4681 tatatttctg ctatagaggt gcgcgataat cagagcgtaa aaaaaggcga tattatcctg
     4741 cgtctcgatg atcgggatta tcgtgcaaat gtcgccaggc ttgaggcaaa aataaaaagt
     4801 agcaaagcca atctcgaagg cattcaagcc actattacga tgcagcagtc gataattcaa
     4861 agtgccagtg aaacatggca ggcagtaaaa catgaggagc aaaagcgcct gcgagatact
     4921 gaacgttatg aaaaactcgc acaatcagcg gcaatatcgc aacaaattat tgataacgcc
     4981 aggtttgact accagcaggt cgcagcaaag gagcgcaaag ccgcgaacga tttcctggtg
     5041 gagaagcaac ggctggcagt tttatctgct caggaagaaa acgtacgtgc gagtatcgaa
     5101 gaagttcagg ccgctttgac tcaggcacta ctggatctgg aatataccct
//
LOCUS       DAZOYF010000228         5076 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZOYF010000228 DAZOYF010000000
VERSION     DAZOYF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.230"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(11..322)
                     /locus_tag="ACHUOF_003932"
     CDS             complement(11..322)
                     /locus_tag="ACHUOF_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR0612890.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="ACHUOF_003933"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHUOF_003933"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="ACHUOF_003934"
     CDS             701..1054
                     /locus_tag="ACHUOF_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR0612891.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHUOF_003935"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHUOF_003935"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFR0612892.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="ACHUOF_003936"
     CDS             complement(2810..3280)
                     /locus_tag="ACHUOF_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR0612893.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHUOF_003937"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHUOF_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /locus_tag="ACHUOF_003938"
     CDS             complement(4119..4337)
                     /locus_tag="ACHUOF_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR0612894.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHUOF_003939"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHUOF_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR0612895.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         1369 a         1173 c         1217 g         1317 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaaga
//
LOCUS       DAZOYF010000229         5047 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZOYF010000229 DAZOYF010000000
VERSION     DAZOYF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.231"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(44..214)
                     /locus_tag="ACHUOF_003940"
                     /pseudo
     CDS             complement(44..214)
                     /locus_tag="ACHUOF_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHUOF_003941"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHUOF_003941"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR0612896.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="ACHUOF_003942"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="ACHUOF_003942"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR0612897.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="ACHUOF_003943"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="ACHUOF_003943"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR0612898.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="ACHUOF_003944"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="ACHUOF_003944"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR0612899.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="ACHUOF_003945"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="ACHUOF_003945"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR0612900.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
BASE COUNT         1274 a         1300 c         1315 g         1158 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaac
//
LOCUS       DAZOYF010000230         5014 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZOYF010000230 DAZOYF010000000
VERSION     DAZOYF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.232"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..297
                     /locus_tag="ACHUOF_003946"
     CDS             <1..297
                     /locus_tag="ACHUOF_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0612901.1"
                     /translation="LFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADN
                     QQVKLSMPELEELAAAMVQAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(498..1355)
                     /gene="sitD"
                     /locus_tag="ACHUOF_003947"
     CDS             complement(498..1355)
                     /gene="sitD"
                     /locus_tag="ACHUOF_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR0612902.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1352..2209)
                     /gene="sitC"
                     /locus_tag="ACHUOF_003948"
     CDS             complement(1352..2209)
                     /gene="sitC"
                     /locus_tag="ACHUOF_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR0612903.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2206..3033)
                     /gene="sitB"
                     /locus_tag="ACHUOF_003949"
     CDS             complement(2206..3033)
                     /gene="sitB"
                     /locus_tag="ACHUOF_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR0612904.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3033..3947)
                     /gene="sitA"
                     /locus_tag="ACHUOF_003950"
     CDS             complement(3033..3947)
                     /gene="sitA"
                     /locus_tag="ACHUOF_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR0612905.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4379..>5014)
                     /locus_tag="ACHUOF_003951"
     CDS             complement(4379..>5014)
                     /locus_tag="ACHUOF_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR0612906.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1323 a         1227 c         1200 g         1264 t
ORIGIN      
        1 ctgttcacgt ttgaatggaa cggtcgtatc tggaatgctg gtcccgactc actgggccgc
       61 ctgtccccgg tagtcatgct ggcaaaatct gtcacagcac aaacacatat ggcgtggagc
      121 gatgccgata atcagcaggt gaaactgtcg atgccggaac tggaagaact ggcggcagca
      181 atggtgcagg cgcaggtcga tcgcaacgat gagatttatc gccgtcagcg ggagctgaag
      241 gaagagctga atagtctgaa ggatttgaat tcggttagga attttatcgt ggaataacag
      301 aagctgcggc acgtcgtatg caggaacgtg ccgcggttgg ctggtaaact ttcgatagtg
      361 cgagtattga atgatttcta ggcgttatcg attttacgta ttttttgcat gatagaattc
      421 gtacctcctc ccaacgacct tccatggctt tccggacttc tgtaggctgg cggacgatgc
      481 tgtcctcgga tgttgtgcta attgggtgat aacagatcct gtgtatgggc gtttcccttt
      541 gtgcgtgcgt tgatgctggt aacggtaaac gtcatgatga acacgaccgc gaataagacg
      601 acaatcgtcg gagctggtgc gctgtcgata aaaaaggaca gccagacacc agtcattgac
      661 actattaccg atatgccagt cgccagtagg agcgcaatat gaaagcgttg tgtcaggagt
      721 actgcaatgg cgccaggcgc aatgagcaga gaaatagaga gaatgatacc tactgctttc
      781 agcgtcgcca caatggtcag ggaaaccatg cacagcagcc cataatgcag ccatctcgta
      841 tgcaaaccgc ttacctgcgc ctgttgataa tcaaagctga acagcaaaaa atcccgccat
      901 tttacgctaa taacaagtgt aaccagcccg gcaataatca ccgtctggat tatgtcgccg
      961 atggttatcc cgagcatgtc gccgaaaaga atatggtcaa gatgtacgtc tggtttaatt
     1021 gcaatataca agatcagacc tgcggcaaac atgccagaaa aaacaatccc cataactgta
     1081 tcttgcttga ttcggctgtt gtctttcagg tatcccgtcg ccaccgcgca gaatactccg
     1141 gcgacgaatg cccctgtagc cagaggtaac cccagaatcc aggtaagcac gatgccagga
     1201 aaaaccgcat gactcatcgc gtcacccatc agtgcccaac ctttcaggac cagaaagacc
     1261 gacagcagcg cacagggtat ggaaactacc agtgaaatca gcagtgcgtg actcataaag
     1321 gtgaactgca gtggctccag taataatgcc atcatgagcg atcctccagc gctttatgcg
     1381 cacgccgacg atttgccaaa agtccatggg tgggcgcaaa tacaaatgct aaaagaaaca
     1441 gcagtgtctg cgccaccacg atgattccac ctgtcgcgcc gtccagaaag taactgaccc
     1501 acgcgccgag gaaactggtt acactgccaa tagttacagc aattatgagc aggcggggaa
     1561 aacgatcggt taacaaccat gctgttgcac cgggagtcac caccagacag atcaccagaa
     1621 aggcaccaac cgtttgcagc gccgcaacgg tcgaaaccga cagcagggtg aagaagagga
     1681 tttttaatct gcccggatgt aatccaatag cacgggcgtg attctcgtcg aaaaaggtca
     1741 ccataagatc tttccatttg aaaaaaagca cgatgatcga gagaatgcca atgatagtga
     1801 gttgcaggat atccgccgga tcgattgcca gaatattgcc caggacgatg gtctgaatgt
     1861 tcacggatgt cgggttcaat gacaccataa acagccccag gccaaaaaag gacgagaaga
     1921 tcaggccgat gatggcatct tcttttaagc gggtgcgttg gttaagaaac agcatgctgc
     1981 ccgctgccag tccgccggaa aagaaggctc ccagagaaaa cggaagcccc agcatatatg
     2041 cccccgcaac acccgggaca atagagtgcg acagcgcatc gccaatcagc gaccagcctt
     2101 ttagcatcag atagcatgac aggaaagcgc agagaccgcc gaccattgcc gagacccaca
     2161 tcgcattgag catatattca tagctgaagg gttccagtag cacgttcatc ttgcttccct
     2221 ctgcaccgct gacggtcgat gtgccacaaa agggcgttca tcgtcagtaa taatgctttc
     2281 ttccgagccg ttaagtgtga catggcgcag tacgccgcta aaagccagct ccaggttctc
     2341 ggctgtaaaa gtggtgtctg tcggcccgct tgccaggacg gtgcctttga ccatgaccgt
     2401 ataatcgcaa aatgtcgtga ctgagccgag attgtgcgtt gagacaagca tcgttttgcc
     2461 ttccgcgcgt aactccctca acaggctgat gattttcgct tcggttttca catcaacgcc
     2521 ggtaaaaggt tcatccagca ggatcacatc accctgctgt gcaattgctc tcgccagaaa
     2581 gacgcgtttc ttttgtccac cggaaagctc gccgatttga cgatggcgaa aatccaccat
     2641 atcgacacgt tccagcgcat cggtgacaat ctgacgatcc ctctttttgg ctatacgcaa
     2701 catacccata tgcccatagc ggcccatcat caccacatct tccaccagta caggaaatga
     2761 ccagtcaacc tcttctgact gagggacgta ggcaaccagg tttttctgta gcgcctgtcg
     2821 cgtgggaata cccagaacag atatctttcc gctggtcaga cgcacaaatc ccataatcgc
     2881 tttaaacaac gtcgattttc cggaaccgtt taccccaacc agagcggcaa tagatccact
     2941 tggtactgtg aaggatgcat cgcgcagtgc tgtgtgcccg ttacgccagg tgacagtaac
     3001 atcattaacg acaatgcctg cagattgcat cattatttct cccttttccc ggctttaatt
     3061 ccctgtacca gcgtactggt agtaacctta agaaggtcga tatacgtagg taccgggccg
     3121 ttttctgtgc tcagggaatc gacatagagc acaccgccgt agtgtgcgcc ggtttcacgc
     3181 gcaacctgac gcgctggttt atcggaaatc gtactctcgc taaagactgc cgggatatga
     3241 tttttcttaa ctatatcaac aaccttacgt acctgctgcg gtgttccttg ttgatcggca
     3301 ttaatcggcc acagataaag ctcttttagc cccaaatcgc gtgcgagata agaaaaagcc
     3361 ccttcactgg tgaccatcca tcgctgattc tcagggagtt ccgtaatctg cttacgcagg
     3421 ggggcaaggg tttgggtaat cttggcttta taagtatcgg cattacgttg gtaggtttgt
     3481 gcatttgccg gatcgtattt tatcaacgca tcacgaatat tatcgacgta aatcagagca
     3541 ttatctggcg acatccatgc atggggatta ggtttgccct catagggtcc ttcggtgatc
     3601 cctactggcg tcacacccga agagacaatt acttctggaa ccccattgag atgctggtaa
     3661 aagcgttgga accacaattc cagattcata ccattggcga gaatcagttg tgccccctgc
     3721 gcacgtttaa tatcgccagg ggtaggctga tactcatgaa tttctgcacc aggcttggtt
     3781 atggatgaga cttctgcagc atctccagcc acgtttttgg ccatatccgc gatgatggta
     3841 aatgttgtaa tgaccttgaa tttttcagtt gcacttgcct gaaatgcgat cgagcaggtg
     3901 agggcgaggc accccaagag catggttact ttttttattg agtgcataat ttagtaccta
     3961 tagtgagcaa aattagaaca aacatagcaa cggctatgtt ttatagcata acctatacaa
     4021 aatgaaaata attatcatta acagctttga ggcctaaaac agggattagg ttgtttcctg
     4081 ctggcgacat gatattgagg aaaaaagtag agcagctatt aggctttgaa atgactttat
     4141 gaggaaaatt gttgatggca aaaatggggc aaaaaagagt gggttatcgt agcttattgt
     4201 tgtcgctgat aatgtttaac atattgaaaa ataaataaaa tacttatgag tcagatagtt
     4261 gtgatttttg cccttacttg ttcaggttgt attgttcttt ctttgtaatt tgttgatttt
     4321 cttgcattat ttcagttctc tggtactaaa tggggcaaat tgggggcaaa ctttgcaact
     4381 acgataaccg cgcattcaac atggctatct gttcgtcgtt catgtcatca atccacatac
     4441 cgtaaatttc atacaccatt tgcgcagttt catgccccat ctggctggct ataaatgccg
     4501 ggttcgctcc tgccgtcaac agccagcagg caaaagtatg tcgcgtatgg tacggattac
     4561 ggcggcgaat accagcacgt tttactgctg cattccatct tgtacccaaa ctgcttaccg
     4621 agtaataagg tttctgtttt tcgttacaca ccctgggcat gaaaacaaaa tgcagttttt
     4681 gtttttcagt tctgccgtac tcccgatgat aaaaagtgat ttcgcttttg cgatgatgcc
     4741 cggtcagttt gtattgctcc ttcagtgctt caagagccgg ctgcagtaat gctaccgtcc
     4801 ggatcccggc atttgttttt gggggaccga acatatcaag tatcgtcagg tttcttctga
     4861 cattcacaat tcccttctcg aaatccacat cctcccacgc cagagctgcc agttccccgt
     4921 gacgaagccc ggagtaaacg gcaaatttcc acaagttttg gctctgtcct ttttcacttt
     4981 ccattaatgc attgaattct gttttagata acgg
//
LOCUS       DAZOYF010000231         4973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZOYF010000231 DAZOYF010000000
VERSION     DAZOYF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.233"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHUOF_003952"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHUOF_003952"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFR0612907.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="ACHUOF_003953"
     CDS             1020..1703
                     /locus_tag="ACHUOF_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFR0612908.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="ACHUOF_003954"
     CDS             1700..2161
                     /locus_tag="ACHUOF_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFR0612909.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="ACHUOF_003955"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="ACHUOF_003955"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR0612910.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="ACHUOF_003956"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="ACHUOF_003956"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFR0612911.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHUOF_003957"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHUOF_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFR0612912.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHUOF_003958"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHUOF_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFR0612913.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(4903..>4973)
                     /locus_tag="ACHUOF_003959"
     CDS             complement(4903..>4973)
                     /locus_tag="ACHUOF_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612914.1"
                     /translation="NYSEYSEYTSVFFLSLMIFIKS"
BASE COUNT         1177 a         1305 c         1238 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctgact caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAZOYF010000232         4945 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZOYF010000232 DAZOYF010000000
VERSION     DAZOYF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.235"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(253..876)
                     /locus_tag="ACHUOF_003960"
     CDS             complement(253..876)
                     /locus_tag="ACHUOF_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR0612915.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1173..1448
                     /gene="ypdI"
                     /locus_tag="ACHUOF_003961"
     CDS             1173..1448
                     /gene="ypdI"
                     /locus_tag="ACHUOF_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR0612916.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(1525..1767)
                     /locus_tag="ACHUOF_003962"
     CDS             complement(1525..1767)
                     /locus_tag="ACHUOF_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR0612917.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            2120..3040
                     /gene="lpxP"
                     /locus_tag="ACHUOF_003963"
     CDS             2120..3040
                     /gene="lpxP"
                     /locus_tag="ACHUOF_003963"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR0612918.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3395..3466
                     /gene="ypdK"
                     /locus_tag="ACHUOF_003964"
     CDS             3395..3466
                     /gene="ypdK"
                     /locus_tag="ACHUOF_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR0612919.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(3531..4769)
                     /gene="alaC"
                     /locus_tag="ACHUOF_003965"
     CDS             complement(3531..4769)
                     /gene="alaC"
                     /locus_tag="ACHUOF_003965"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR0612920.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
BASE COUNT         1292 a         1207 c         1115 g         1331 t
ORIGIN      
        1 ctgtaagaag cgccggtgat ttacaagtaa gagtatgatt acgcttttta aagcgtacca
       61 taaagcataa cataataaat gatttgtgat gacacgggat tattgcggtt tactttgagg
      121 aaaataaaat gcgtgtttat agaaccgcga gcgccaaata attttatgac cacaacctgt
      181 cgtattgtgg tcataaaaaa ttgtcggctg gagggagtta aatcctttac ctcaatatac
      241 tgcctgtcag cattagttca caaacagtgc ttcgctatcg acgatgattc catcttccgc
      301 acctttaagc gccaggtttg cctcgtagta ttgcttttta tcaagcagtt tctgcgcatc
      361 agcaagtgca ttacgagtct gtttaagcgg catcagatat tggttttcca ttacgccaac
      421 accggccagg cgaagctctt ccattgcgcc ctttttatcc cctttcgcca ttttttcatt
      481 ggcaattttt atcgcggctt ctttttcggg tgttgctaca taactctcag aaattccgac
      541 agatgcatta atgacaatat attggtcatc attgagattg gtctttttac ctggcttagc
      601 gaactttgcc cattcggtac tgtcatctga cagcaaagcg gaagcttcat tggtcagttc
      661 ttttgccttt tccggatcgc catgaaacag agccaggcgc gctacctgca cgtcacgcat
      721 cgcatataaa ccttgttcag agatacgctc tgctgctgcc gttttctggg tttgctgagt
      781 ttgctgttgt gccgctaccg gtgcattatc agcagcccat acagttgaag atgccagaat
      841 tgctgtgacc atcgtagcca taattaaacg tttcatactt acctcattct ttaacagttt
      901 tagaaaacgc cgccatcata ttgggtgagc atgaaatgag aaatcagaga gcaacggtca
      961 ttttgaaagg gttatcttac aattgtagtt attatcgcaa agcggcattt agtcttacag
     1021 ttgtaggaat atgcctcctg tcgcagagaa ctattcttat ataccacaaa gagatgtcat
     1081 ttattatatt gatgtaaata ttgattaatt atatgtaaat acagccataa catcgtatgg
     1141 ttctcagtga accattttca ggataacttt ctatgaaagt aaacttaata cttttcagct
     1201 tatttttatt ggtctctatt atggcatgca atgtttttgc attttccatt tctggtggtg
     1261 taagtgagag gagctataaa gagactgaaa aaacatcagc gatgacgacc acacactcta
     1321 caaaacttca gccatcacag gcgattttgt ttaagatgag agaagatgtg ccaccattaa
     1381 acctcacaga agaaatcacg cctacttatc cgacaaaggc gaattatctt attcatcctg
     1441 tgcgatagcg tctgtcacat ggcaggtgaa caatcatgtc tgatgctgat gtgaacacct
     1501 gcccaaatga gccatcctga cccactatcg cctgccatac cccaccataa agcgaatcat
     1561 caagccacaa gccggggcaa gaaacataaa tatccccgtc accagttcat taccgccagt
     1621 taaccacatg gtgaagtaat aaatgagtgc agcgaggatc accatgccga agaaagaaga
     1681 gaccgccgat accacgttca gtacctgacc gatgtagccg cttacgcaga caatacacag
     1741 ggcgagaaac atccataaat aaatcatagc tcaacctcct gccgcgctaa aaaacctgta
     1801 acttcagtca ctttaatagt aagacaatcc ttaacctctg gcaatttttg ctaagcatca
     1861 atgaaaacag cacgttaaat gcaacgctat tgcgtgcgga aaatttttta ctttgcacga
     1921 ttaataatca gtagctgaaa gcagtcagcc agtgataaag tagagccacc gcattcatgc
     1981 tcctcgtggt tatgtcctga ctagtcttta cactctttac aggaaccatt gtcgtacatg
     2041 atggcccaac caattgagtg taccgctgtc gacatggaat cttctgcgat acaacaattc
     2101 gtatctacag aaggtaacta tgtttccaca atgcaaattt tcccgcgagt ttctacatcc
     2161 tcgctactgg ctcacatggt ttgggcttgg tgtactctgg ctttgggtac agcttcctta
     2221 tcctgttctc tgctttctcg gcacgcgtat tggcgcaatg gcgcgaccat tcctgaaacg
     2281 tcgtgaatct atcgcccgta aaaacctgga actttgtttc ccgcagcatt ctgcggaaga
     2341 acgcgagaag atgattgccg aaaactttcg ttcactcggc atggcgctgg tagaaaccgg
     2401 catggcatgg ttctggcccg acagtcgcgt acgtaaatgg tttgatgttg aagggttgga
     2461 taaccttaaa cgcgcacaaa tgcaaaatcg cggcgtaatg gttgtcggcg tccattttat
     2521 gtcgctggaa ctgggcggcc gcgtgatggg actgtgccaa ccaatgatgg ctacctatcg
     2581 tccacataat aatcagctga tggaatgggt gcagacccgt gggcgcatgc gctctaacaa
     2641 agcgatgatc ggcagaaata atctgcgcgg cattgtcggt gcactgaaga aaggtgaagc
     2701 ggtatggttt gctcccgatc aggattatgg tcgtaaaggc agctccttcg cgccgttctt
     2761 tgcggtggaa aatgtcgcca caaccaatgg cacctatgtt ctctcccgtc tctctggcgc
     2821 agccatgttg accgtaacga tggtaagaaa agcggattac agcggatatc gtttgttcat
     2881 caccccagag atggaaggct acccgacaga tgaaaatcaa gccgctgctt atatgaacaa
     2941 gattatcgag aaagagatca tgcgcgcacc ggagcagtac ctctggatcc accgtcgctt
     3001 taaaacgcgc ccggtgggag aatcgtcgtt gtacatttaa caaactgctc ttactctttc
     3061 cctcctcaaa tcggccataa taacctcatg atttttatag cttttttctt aagttttttc
     3121 cttcgctgaa tatctaatta ttaccgtcag aaattaaact gtcgccgata tgagacactt
     3181 gtaagttact aaaagtaaat aagaaaatgc ctcttgtctc gtttcagttt taggcgtaca
     3241 ttagcgccgt ctcgagcagt ttagctcaga attctggggt gatgcccacg gtaaaataca
     3301 gggcttttaa ggtcgctttg cccggtgtca actcactgta tcaggtgtaa tgaagtcatt
     3361 caggcgtaac agtaattacg cggagagatg taaagtgaaa tatttcttta tgggcatttc
     3421 ttttatggtc atcgtttggg ccggtacttt tgccctgatg atctaaaagc aaactgtgca
     3481 atgaaaaaca ggagccatca ggctcctgtt tgcatttctg acgacggtgt ttattccgcg
     3541 ttttcgtgaa tatgtttgct gctgacgggt agtaaaccgt cggcacggaa catcgcttta
     3601 atccctctga tggcctgacg aatacggtcg tggttttcaa tcagtgcaaa gcgaacatgc
     3661 gtatcaccat agtcgccaaa gccaatcccc ggcgagacac aaaccttcgc ttcgttaagg
     3721 agcttcttgg caaattccag cgatcccatg gccgcatatg gttccgggat tttcgcccag
     3781 acatacatcg aagccttcgg catttcgacc atccagcccg cttcatgcag ccctttaacc
     3841 aatacatcac gacggcgttt gtactgttcg gcaatgtcgc gcacgcactg ttgatcgccc
     3901 tcgagtgccg caatcgccgc cacctgcaac ggcgtaaaag tgccgtaatc gtgatagctt
     3961 ttgattcgcg ccagggcact gaccagcgtt ttgttgccga ccataaagcc gatacgccag
     4021 cccgccatgt tgtagctttt cgacagcgta aagaactcga ccgccacatc gcgtgcaccc
     4081 ggcacctgca taattgacgg cgctttccag ccatcgtaga cgatatcggc ataggccagg
     4141 tcatggacca ccagcacatc gtagcatttc gccagcgcca caaccttttc gaagaactcc
     4201 agttccacgc attgcgcggt tgggttcgac gggaagccga ggatcatcat cttcggtttc
     4261 ggataacttt cacgaatggc gcgctccagt tcgttgaaga aatcgacacc ttccaccagc
     4321 ggcactgagc gtacctgcgc cccggcaatc accgcgccat aaatatggat cgggtaactt
     4381 ggattcggca ccagcaccgt gtcaccatga tccagcgtcg ccagcatcag atgcgccagg
     4441 ccctctttcg aaccaatagt gacgatggct tctgattccg ggtcgatttc aacgtcgtag
     4501 cgatcctgat accagcggga aatggcgcga cgtaaccgcg gaatgccgcg tgaagtggag
     4561 taaccatgcg tgtccgggcg ctgggccaca gtacataatt tttcgacgat atgcggcgga
     4621 gtcgcaccgt ccgggttacc catgctgaaa tcgataatat cttcgccgcg ccgacgcgca
     4681 gccattttca gttcagcggt gatgttaaaa acgtagggcg ggagacgatc aatgcgcgta
     4741 aagcgacgtt cagggcgagt gtcagccata atttcctcag attaacgtta gcgcccggac
     4801 cgtccgagcg acgctgccac aatggtggct gttttgaaaa tagcctgatt aatttctgtc
     4861 tgtccagcgt tttagaacat ttttatcaat ggatttaaat gaggaactcc acttcaaaaa
     4921 caggaaattt ttctacgcta acaag
//
LOCUS       DAZOYF010000233         4930 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZOYF010000233 DAZOYF010000000
VERSION     DAZOYF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.236"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            298..1683
                     /gene="dcuC"
                     /locus_tag="ACHUOF_003966"
     CDS             298..1683
                     /gene="dcuC"
                     /locus_tag="ACHUOF_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0612921.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1724..2065)
                     /locus_tag="ACHUOF_003967"
                     /pseudo
     CDS             complement(1724..2065)
                     /locus_tag="ACHUOF_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2403..2912)
                     /locus_tag="ACHUOF_003968"
     CDS             complement(2403..2912)
                     /locus_tag="ACHUOF_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0612922.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            3084..3320
                     /locus_tag="ACHUOF_003969"
     CDS             3084..3320
                     /locus_tag="ACHUOF_003969"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0612923.1"
                     /translation="MSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVRPWP
                     KGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            3295..4926
                     /locus_tag="ACHUOF_003970"
                     /pseudo
     CDS             3295..4926
                     /locus_tag="ACHUOF_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1228 a         1095 c         1225 g         1382 t
ORIGIN      
        1 atatcgtcaa cccatgctca tattagatta ttttttgagc gcacagccgc agctaacacc
       61 attgcaatta acaaatttgc atcaatccac catcaattta cacacattat tatgtgataa
      121 ttgccaaccg ctaaatatgc gttttgttat ctatgtataa aaacagcaac ttcaatgtct
      181 taatggcagt ttttcttgat tttaatcagc attcatcgcc aatttattgg gcatattttt
      241 tccttaagct ttaggaagtt tttattattt actttgggcc tggagacagg aaaaattatg
      301 ctgacattca ttgaactcct tattggggtt gtggttattg tgggtgtggc tcgctacatc
      361 attaaagggt attccgccac tggtgtgtta tttgtcggtg gcctgttatt gctgattatc
      421 agtgccatta tggggcacaa agtgttaccg tccagccagg cttcaacagg ctacagcgcc
      481 acggatatcg ttgaatacgt taaaatatta ctaatgagcc gcggcggcga cctcggcatg
      541 atgattatga tgctgtgtgg ctttgccgct tacatgaccc atatcggcgc gaatgatatg
      601 gtggtcaagc tggcgtcaaa accattgcag tatattaact ccccttacct gctgatgatt
      661 gccgcctatt ttgttgcttg tctgatgtca ctggccgtct cttccgcaac cggtctgggg
      721 gttttgctga tggcaaccct gtttccggtg atggtaaacg ttggtatcag tcgtggcgca
      781 gcagctgcca tttgtgcctc cccggcggcg attattctcg caccgacttc aggggatgtg
      841 gtgctggcgg cgcaggcttc cgaaatgtcg ctgattgact tcgccttcaa aacgacgctg
      901 cctatctcaa ttgctgcaat tatcggcatg gcgatcgccc acttcttctg gcaacgttat
      961 ctggataaaa aagagcacat ctctcatgaa atgttagatg tcagtgaaat caccaccact
     1021 gcccccgcgt tttatgccat tttgccgttc acgccgatca tcggtgtact gatttttgac
     1081 ggtaaatggg gtccgcaatt acacatcatc actattctgg tgatttgtat gctgattgcc
     1141 tccattctgg agttcctccg cagctttaat acccagaaag ttttctctgg tctggaagtg
     1201 gcttatcgcg ggatggcaga tgcgtttgct aacgtggtga tgctgctggt tgccgctggg
     1261 gtattcgctc aggggcttag caccatcggc tttattcaaa gtctgatttc tatcgctacc
     1321 tcgtttggtt cggcgagtat catcctgatg ctggtattgg tgattctgac catgctggcg
     1381 gcagtcacga ccggttcagg caatgcgccg ttttatgcgt ttgttgagat gatcccgaaa
     1441 ctggcgcact cttccggcat taacccggcg tatttgacta ttccgatgct gcaggcgtca
     1501 aacctgggtc gtaccctatc acccgtttct ggcgtagtcg ttgcggttgc cgggatggcg
     1561 aaaatctcac catttgaagt cgtaaaacgc acctcggtgc cggtgcttgt tgggctagtg
     1621 attgttatcg ttgctacaga gctgatggtg ccaggaacgg cagcagcggt cacaggcaag
     1681 taaatagtaa tgccggaggc aagtttcctc cggctttatc atgtcaccca ctgtggtata
     1741 tgcgttgtgg tctgccaact ttgccgtgaa caatttcagc aataatcaga tggcggctgg
     1801 cgcaatattc aagataacgc ctggcagtgg tgcggctgat ggtcagtgcc tgcgccaccg
     1861 tttctgccgt atgttgcaca ccaggctctt taaacagttt tcgcaccgcg tttagcgtca
     1921 aggggtcaat gccggtcggc agttcgtctt tgggttcacc gcgagcatag gcattaaaca
     1981 tctcatcaat ttgcttctgg ctggcgctat caatactttc cagcatatgt ttacgctggc
     2041 ggaaacgggt tagcgtttgc cccagtgtat tgattcactt gaagtacgaa aaaaccggga
     2101 gaacattgga ttattcggga tctgatggga ttagatttgg tggggcttgc aagcctgtag
     2161 tacaaatttt aggcgttaac caatgaaacg cgaaagatag taaaaaattg cttttgtttc
     2221 attgaaaaaa cgaaaaacaa aaacactata aatcatttca atcatagctt caaaaaacgt
     2281 tcaaaaccga taacaaccag gctgtcatca aatgactcat atcacaaatc agctcatgcc
     2341 gtttaggtat gttacatgtg tgattatgtg aggtgaagta tgttttagct ggctcatggt
     2401 tgttatacgg cttttttcgc atcttgtggt tccaacaaag gagctacagg ctcttcctgt
     2461 ttatgaacac catacattga caaaatctct tggcgagttt ctggaggtaa tttatgcagc
     2521 gccaaagctg tttgtagtgt ttggcgatca atacccagcg ttgccaatag ggtgtcaacg
     2581 gagatggagg ggcgttcttg cattaatccg gaaagcccat atttagccat gtttagctct
     2641 atctctcgga cttcattttc tcctagttta ttgattaaca gggagaacat tttcaatgta
     2701 tcagcattca tttcttcgat agcaattttt tgcatagccg gtattgccgg gatatattca
     2761 accccaggcc cgcgacgccc ttcttgtgaa cgcttcggcc atccttcttt ctttgctcgt
     2821 ttatgcaccc cctgtattgt cccaggcatt ccatccagtt cagtaatttg tttgggtgtc
     2881 atccactggt ttatctcatt tgcgcttttc ataaactaag tttaccagat ttatctatat
     2941 aaaaatcaat ggattacaaa ataaaccaca ttaactgata taaaccggtt tggtttattt
     3001 ggtaatgtat aaaacagata atcaagacga attatttgtt ttggtaaccc tttatcatac
     3061 gcaatatggt tggtgtgcga aagatgagta acgattggca tcgggccgac gtgattgcgg
     3121 ccctcaaaaa aacaggaacc cctctggcaa aattgtccag acgttcaggt tattcaaccg
     3181 ggacgcttgc aaatgcatta gtcaggccat ggcctaaagg tgaacgcatt attgctgacg
     3241 ccttaaacat gaaacctgag gaaatttggc ctagtagata tctaagggat cacaatgaga
     3301 gagtggatat cagcaattga atgcatgggt ttatctggtt tacctggttc attaatgggg
     3361 gttcacaaaa gagctcagag agaaggatgg ctcaagagaa gaagagaagg aattagaggg
     3421 gttgcttacg aataccatat atcttctctt cctaacattg ctcgagctga agttttgctc
     3481 caacaaggaa aatttaaaac cagtcaggga tgttttgaaa tcgcccgccc cacgctggaa
     3541 gctcatgatt atgatcgtga ggcactatgg agcaaatggg ataaagccag cgattcccag
     3601 cgcagcctgg ctgaaaaatg gttgccctca attcaggcca cagacgaaat gctgaaccag
     3661 gggatttcaa cgaaaacggc ttttgcgacc gttgcagggc attaccaggt cagcgcatcc
     3721 actttgcggg acaagtatta ccaggtacag aagtttgcga agcctgactg ggcggctgca
     3781 cttgttgatg gacgtggagc atcccgtcgc aatgttcaca aaagtgaatt tgacgaggat
     3841 gcctggcagt ttctgattgc agattatctg cgaccggaaa aacccgcctt ccgcaaatgt
     3901 tatgagcgtc tggaactggc agcccgcgag catggctgga gtattccctc ccgtgccacg
     3961 gcctttcgcc ggattcagca actggacgag gcaatggttg ttgcctgtcg tgaaggtgaa
     4021 catgcactga tgcatctgat accggcacag cagcgaactg tggaacacct ggacgccatg
     4081 cagtggatca acggcgacgg ttatctgcat aacgtctttg tacgctggtt taacggtgat
     4141 gtgatccgtc cgaaaacatg gttctggcag gatgtgaaaa cccgaaaaat tctgggctgg
     4201 cgctgcgatg tgagcgagaa cattgattca attcgcctct cgttcatgga tgttgtgact
     4261 cgctacagta tcccggagga ttttcacatc accattgata acacccgtgg tgctgcgaat
     4321 aaatggctga cgggaggcgc gcccaatcgc taccgcttta aggtaaaaga ggacgatcca
     4381 aaaggactgt ttttactgat gggtgcgaaa atgcactgga caagcgttgt tgccggtaaa
     4441 ggctggggcc aggcaaaacc tgttgaacgt gctttcggtg ttggtgggct tgaggaatac
     4501 gttgataagc atccggcact ggctggcgca tatacggggc caaatccgca ggcaaaacct
     4561 gataactatg gcgaccacgc tgttgatgca gagctgtttc tgaaaacctt tgccgaaggt
     4621 gtggcgatgt tcaatgccag aacaggccgt gaaacagaaa tgtgcggggg caaactctcg
     4681 tttgatgatg ttttcgagcg tgaatacgcc agaacgattg tgcgtaagcc aaccgaagaa
     4741 caaaaacgga tgctgttact gcctgccgag gcggtgaacg tttcacgcaa aggcgagttt
     4801 acgcttaaag ttggcggctc ccttaaaggc gcgaaaaacg tttattacaa catggcatta
     4861 atgaatgccg gagtgaaaaa agttgtggtc agatttgatc cacagcagtt acacagcacg
     4921 gtttattgag
//
LOCUS       DAZOYF010000234         4870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZOYF010000234 DAZOYF010000000
VERSION     DAZOYF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.237"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..527
                     /gene="ydcF"
                     /locus_tag="ACHUOF_003971"
     CDS             <1..527
                     /gene="ydcF"
                     /locus_tag="ACHUOF_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR0612924.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="ACHUOF_003972"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="ACHUOF_003972"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR0612925.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHUOF_003973"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHUOF_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="ACHUOF_003974"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="ACHUOF_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR0612926.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="ACHUOF_003975"
     CDS             4169..4342
                     /locus_tag="ACHUOF_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612927.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4368..4436
                     /gene="yncP"
                     /locus_tag="ACHUOF_003976"
     CDS             4368..4436
                     /gene="yncP"
                     /locus_tag="ACHUOF_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR0612928.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHUOF_003977"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHUOF_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR0612929.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="ACHUOF_003978"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="ACHUOF_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1120 c         1208 g         1295 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     4861 aatactagtt
//
LOCUS       DAZOYF010000235         4801 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZOYF010000235 DAZOYF010000000
VERSION     DAZOYF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.238"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(89..2962)
                     /gene="gcvP"
                     /locus_tag="ACHUOF_003979"
     CDS             complement(89..2962)
                     /gene="gcvP"
                     /locus_tag="ACHUOF_003979"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR0612930.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(3081..3470)
                     /gene="gcvH"
                     /locus_tag="ACHUOF_003980"
     CDS             complement(3081..3470)
                     /gene="gcvH"
                     /locus_tag="ACHUOF_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR0612931.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(3494..4588)
                     /gene="gcvT"
                     /locus_tag="ACHUOF_003981"
     CDS             complement(3494..4588)
                     /gene="gcvT"
                     /locus_tag="ACHUOF_003981"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR0612932.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
BASE COUNT         1080 a         1358 c         1243 g         1120 t
ORIGIN      
        1 ggcgagggga ctgtctgagc acttttgtac tttgtcatct gactaaaaag gcgccgaagc
       61 gcctttagaa aatactcgaa tcagtgaatt actggtattc gctaatcggt acgcaggagc
      121 agaacaggtt acggtcgccg taaacatcat ccagacgttt caccgtcggc cagtatttgt
      181 ctgccacgcc tgccgggaat accgcaactt cacggctgta cggatgcgcc cactcggcga
      241 ccagttcgct ctgaatgtgc ggcgcgttca ccagcgggtt atcttccagc ggccagacac
      301 cggctttcac ctggtcaatt tctgcgcgga tagccagcat cgcgtcgata aagcgatcca
      361 gttccacttt gctttcagat tcagtcggtt caaccatcag cgtacccgcc accgggaacg
      421 acatcgtcgg cgcgtggaaa ccgtagtcga tcaggcgctt ggcaatgtcc agctcgctga
      481 tgccggtttc ttctttcagc gggcgaatat cgagaataca ttcgtgcgcc acgcgaccgt
      541 cgcgaccggt atacagcacc gggaaggcat cctgcaggcg gctggcaata tagttggcgt
      601 tgaggattgc cacctggctt gcttttttca gcccttctgc gcccatcatg cggatgtaca
      661 tccagctgat tggcaggata gaggcgctac cgaacggtgc cgcagaaact gcgccctgac
      721 gggttaacat gccttcgatt tgcaccacgc tatgacccgg tacaaacggt gccagatgcg
      781 ctttcacacc gatcggtccc atacccggac caccaccgcc gtgcggaatg cagaaagttt
      841 tatgcaggtt gaggtgcgaa acgtccgcgc caataaagcc cggcgaggtg atgccaacct
      901 gggcgttcat gttcgcgcca tcaaggtaaa cctgaccgcc gaactgatgc acgacttcac
      961 acacttcacg gatcgtttct tcatacacgc cgtgggtaga aggataagtc accatgatac
     1021 aggagaggtt atcgcccgcc tgttccgctt tcgcgcgcag atcagtcaga tcgatgttgc
     1081 cgtttttatc acacgccaca accaccacct gcattcctgc catatgtgca gaagcggggt
     1141 tagttccgtg cgcagaagcc gggatcaggc agatatcgcg atgcccttcg ttgcggcttt
     1201 catgataatg acgaatcgcc agcaggcccg cgtattcgcc ctgtgcgccg gagttcggct
     1261 gcatacaaac ggcgtcgtaa ccggtcagtt tcaccagcca gtcagccagc tgcgcaatca
     1321 tctgctgata accttcagcc tgttccggcg ggcaaaacgg gtgcagttcg gcaaattccg
     1381 tccaagtgat tgggatcatc tcggcggcgg cgttcagttt catggtgcag gaacccagcg
     1441 ggatcatcgc ctgattcagc gccagatctt tacgctccag cgagtgcata tagcgcatca
     1501 tttcggtttc gctgtgatag cgattaaaca ccggatgggt gaggatttcg tcgtcgcgca
     1561 gcatcgcagg ctggatagag cggctgtcgt gagccacgtc tttgtccagc gtgtcgatct
     1621 ccagcccgtg gttatcgccc agcagcacac tgaaaagctg cattacgttt tcacgcgtgg
     1681 tggtttcatc aagggtgatc cccacggcgt tcaggatatc gctacgcagg ttgatttcag
     1741 ccgcttcggc acgcgtcagt acgcccgctt tgtcggccac ttccacacac aaggtgtcga
     1801 aatagtgcgc atggcgcagt ttcagacctt tttgttgcag gcccgccgcc agaatatcgg
     1861 tcagacggtg aatgcggtta gcgatacgtt tcaggccaac cgggccgtga taaacggcat
     1921 acaggctggc gatgtttgcc agcagtacct gggaagtaca aatgttggag ttcgctttct
     1981 cacgacgaat atgttgctcg cgagtctgca tcgccatgcg cagcgcggta ttgccagctg
     2041 catctttcga tacaccgata atacggcccg gcattgagcg tttgtattca tctttcgccg
     2101 caaagaatgc cgcgtgtggg ccaccgtagc ccatcggcac gccgaagcgt tgcgccgaac
     2161 caaaaacaat atccgcgccc tgtttacccg gcgcagttaa cagcaccagc gccataatat
     2221 cggcggcaac gctgaccaca attttgcgtg atttcagttc gctaataagc gcagtgtagt
     2281 cgtgaatttc accggtagtg cctacctgct gtaacagcac gccgaagacg tcctgatggt
     2341 cgagcacttt ttgcgcgtca tcgacaatca cttcaaaacc aaaggtttca gcacgagtac
     2401 ggaccacatc cagcgtttgc ggatgcacat tggaagccac gaagaagcgg ttggcatttt
     2461 tcagtttgct gacgcgtttc gccatcgcca ttgcttcggc ggcagcggtg gcctcgtcca
     2521 gaagagaagc agaggccata tccagtccag tcaaatccag cgttacctgc tggaagttga
     2581 gcagtgcttc aaggcggccc tgggagactt caggctgata cggagtgtac gcggtatacc
     2641 agcccggatt ttccagcatg ttacgcagga taaccggcgg tagctgcacg gcggtgtaac
     2701 ccatgccgat gtaagacgtg aagcgtttat tgcgactggc aatagccttg agttctgcca
     2761 gtgcggcgta ttcggtcgcc ggtgcgccaa cctgcggtgg tgtcgcaagc tgaatatctt
     2821 tcggcacaat ctggccggtc agcgcgttta acgattgtgc accaacggca ttcagcattt
     2881 cttgctgttg cgcggcgtcc ggtccgatat ggcgttcaat aaaagcgccg ctgttttcaa
     2941 gctggcttaa cgtctgtgtc atgagcgatg gttcctgaaa cgtgcagtga attgtgaacc
     3001 tctctcctta cgaagagagt gagggtgagg cataaatttt cctcaccctg atcctctccc
     3061 gcagaagagg aataaagccg ttactcatct tctaacaatg cttcgtatgc ggtcgcatcc
     3121 agcagtgatt ccagttcgct ttcatcgctg gctttgattt taaagatcca gccgcctgca
     3181 tacggttcgc tgttcaccag ttccggggaa tcgctcagtg cgtcgtttac cgccacgatt
     3241 tcaccgctta ctggcgcata aatgtctgac gccgctttta ccgattcggc aaccgcgcag
     3301 tcatcgcccg cgctaaccgt tgcgcccact tccggcaggt caacaaacac catatcgcct
     3361 aacagctcct gagcatgttc ggtaatacca acggtgtaag tgccgtcggc ttctttacgc
     3421 agccattcgt gttctttgct gtatttcagt tctgctggta cgttgctcat caatcaatct
     3481 ccaaaaaagt aaatcacgcg acggctttgc cgttacgcac aaaaacaggt tttgtcactt
     3541 taaccggcat ttcacggttg cgaatttgca caatcgccgt ttcgccaata ccttccggca
     3601 cgcgcgccag cgcaatgctg taacccagcg tcggggagaa agtaccgctg gtgataatgc
     3661 cttcatgctg gttgccctgc gcatcggtaa agcgtaccgg cagttcatta cgcagcacgc
     3721 ctttttcggt catcaccaga ccaaccagtt tttctgtacc atgctcacgc tgcacttcca
     3781 gggcttcacg accgataaag tcacgatctg tcggttccca ggcgatagtc cagcccatgt
     3841 tggcggctaa aggagaaata gtttcgtcca tctcctgacc ataaagattc atgcccgctt
     3901 ccagacgcag cgtgtcacgc gcccccagac cgcagggctt aacgcccgct tccaccagcg
     3961 cacgccagaa atcggccgct ttttcattgg gcagcgcaat ttcatagccc gcttcaccgg
     4021 tataaccagt ggtggcaata aacagatcgc ccacctgcac gccaaagaac ggtttcatcc
     4081 cttccaccgc ctgacgctga gcatcattaa acagtgtggc agcttttgcc tgagcattcg
     4141 gcccttgcac ggcaatcatg gaaaggtcat cacgaacggt aatttcgatg ccgaaaggtt
     4201 cagcgtgttg ggtaatccag gagaggtctt tttcgcgggt ggcggagtta acaacgaggc
     4261 ggaagaaatc ttcagtaaag tagtagacga tgagatcatc tatcacaccg ccagaggcat
     4321 tcaacatccc cgagtaaagg gctttgccgc ttttggtgag cttcgccaca tcgttcgcca
     4381 gcagataacg cagaaactcc cgggtgcggc tgccgcggag atcgacgatg gtcatatgcg
     4441 acacatcaaa cattccggca tcggtacgta ccgcatgatg ttcgtcgatt tgcgaaccgt
     4501 aatgcagcgg catcatccag ccgtggaaat ccaccatgcg agcgccgcaa agcgtgtgtt
     4561 gttcgtacaa aggagtctgt tgtgccatct tgtcctcatt gaataagcgg ggctgacaac
     4621 tttttcatgg tgaaattatc accacaaaac ccagcatcgg agccactccc ggtccccaac
     4681 gcaatcgttc tcttttgcct gaacttacca ccgaaacaga ctgttaacca taaggtaaaa
     4741 ttgatcatca cattagctta tggttaaaaa atgcaaaaat cgcgacagaa taaaaaacca
     4801 a
//
LOCUS       DAZOYF010000236         4653 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZOYF010000236 DAZOYF010000000
VERSION     DAZOYF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.239"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            240..2672
                     /gene="hsdR"
                     /locus_tag="ACHUOF_003982"
     CDS             240..2672
                     /gene="hsdR"
                     /locus_tag="ACHUOF_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFR0612933.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="ACHUOF_003983"
     CDS             2739..4208
                     /locus_tag="ACHUOF_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR0612934.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..>4653
                     /locus_tag="ACHUOF_003984"
     CDS             4208..>4653
                     /locus_tag="ACHUOF_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR0612935.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGY"
BASE COUNT         1341 a         1146 c         1129 g         1037 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttg
//
LOCUS       DAZOYF010000237         4602 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZOYF010000237 DAZOYF010000000
VERSION     DAZOYF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.240"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(78..866)
                     /gene="phoH"
                     /locus_tag="ACHUOF_003985"
     CDS             complement(78..866)
                     /gene="phoH"
                     /locus_tag="ACHUOF_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR0612936.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="ACHUOF_003986"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="ACHUOF_003986"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR0612937.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="ACHUOF_003987"
     CDS             complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="ACHUOF_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR0612938.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3929..>4602)
                     /gene="efeU"
                     /locus_tag="ACHUOF_003988"
     CDS             complement(3929..>4602)
                     /gene="efeU"
                     /locus_tag="ACHUOF_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR0612939.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1116 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     4021 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     4081 agtgagttga gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     4141 cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa
     4201 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     4261 ggaagccgag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     4321 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     4381 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     4441 tatcgactgc ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa
     4501 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     4561 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat cc
//
LOCUS       DAZOYF010000238         4552 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZOYF010000238 DAZOYF010000000
VERSION     DAZOYF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.241"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..1113
                     /locus_tag="ACHUOF_003989"
                     /pseudo
     CDS             1..1113
                     /locus_tag="ACHUOF_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            1202..1735
                     /gene="blc"
                     /locus_tag="ACHUOF_003990"
     CDS             1202..1735
                     /gene="blc"
                     /locus_tag="ACHUOF_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0612940.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1732..2049)
                     /gene="sugE"
                     /locus_tag="ACHUOF_003991"
     CDS             complement(1732..2049)
                     /gene="sugE"
                     /locus_tag="ACHUOF_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0612941.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2225..2371)
                     /gene="ecnB"
                     /locus_tag="ACHUOF_003992"
     CDS             complement(2225..2371)
                     /gene="ecnB"
                     /locus_tag="ACHUOF_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR0612942.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2482..2607)
                     /gene="ecnA"
                     /locus_tag="ACHUOF_003993"
     CDS             complement(2482..2607)
                     /gene="ecnA"
                     /locus_tag="ACHUOF_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR0612943.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(2659..3225)
                     /gene="efp"
                     /locus_tag="ACHUOF_003994"
     CDS             complement(2659..3225)
                     /gene="efp"
                     /locus_tag="ACHUOF_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR0612944.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3267..4295
                     /gene="epmB"
                     /locus_tag="ACHUOF_003995"
     CDS             3267..4295
                     /gene="epmB"
                     /locus_tag="ACHUOF_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR0612945.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1172 a         1156 c         1131 g         1093 t
ORIGIN      
        1 cgccttatta attaccgcct cttactccac atttgctgcc cctcaacaaa tcaacgatat
       61 tgtgcatcgc acaattaccc cgcttataga gcaacaaaag atccccggta tggcggtggc
      121 ggtaatttat cagggtaaac cttattactt tacctggggc tatgcggaca tcgccaaaaa
      181 gcagcccgtc acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa catttacggg
      241 cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc ccgcaacaaa
      301 atactggcct gaacttaccg ctaaacagtg gaatgggatc acactattac atcttgcgac
      361 ctacaccgct ggcggcctgc cattgcaggt gccggatgaa gtgaaatcct caagcgactt
      421 gctgcgcttc tatcaaaact ggcagcctgc atgggctcca ggaacacaac gtctgtatgc
      481 caactccagt atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt tgagttttga
      541 gcaggcgatg caaactcgtg tcttccagcc actcaaactc aaccatacgt ggattaatgt
      601 acctccccca gaagaaaaga attacgcctg gggatatcgc gaaggtaagg cagtgcatgt
      661 ttcgccaggg gcgttagatg ctgaagctta tggtgtgaag tcgaccattg aagatatggc
      721 ccgctgggtg caaagcaatt tacccccctt gatatcaatg agaaaacgct tcaacaaggg
      781 atacaactgg cacaatctcg ctactggcaa accggcgata tgtatcaggg cctgggctgg
      841 gaaatgctgg actggccggt caatcctgac agcatcatta acggcagtga caataaaatt
      901 gcactggcag cacgcccggt aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca
      961 tgggtacata aaacgggggc gaccggcgga tttggtagct atgtcgcgtt tattccagaa
     1021 aaagagctgg gtatcgtgat gctggctaac aaaaactatc ccaatccagc gagagtcgcc
     1081 gccgcctgtc agattctcaa cgctctacag taaaatttca tcgggtccga attttcggac
     1141 cttttctgcg cttttccttg ctgtcatcta cacttagaaa aaaaccagta aggaaacatt
     1201 tatgcgcctg ctccctctcg ttgccgcagc gacagctgca tttctggtcg ttgcctgtag
     1261 ttttcctacg ccgccgcgtg gcgtgaccgt agtgaataat ttcgacgcca aacgttatct
     1321 cggtacctgg tatgagattg cccgttttga tcaccgtttt gaacgtggac tggaaaaagt
     1381 caccgcaaca tacagcctgc gtgatgacgg cggcctgaat gtcattaata aaggctataa
     1441 ccctgacaga ggaatgtggc agcagagtga agggaaagcg tactttaccg gcgcaccaac
     1501 tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat ggcggttata acgttattgc
     1561 actcgatcgg gaataccgcc atgcgctggt ttgcggcccg gaccgcgact acctgtggat
     1621 actctcccgc acgccaacca tttctgacga agtgaaacag gagatgctgg cagtcgcgac
     1681 ccgggaaggg tttgatgtca gtaagtttat ttgggtacag cagcccggta gttagtgagt
     1741 gctgagtttc agaccaataa tccccaatac gattaacgcc agactcgcca gacgcatcgg
     1801 gttagcggac tcaccgagca gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc
     1861 cgtccacacg gcataagccg tccctaccgg taacgatttc atcgcccagg caagtagcgc
     1921 catactgact atcatcgccg tcacggtaat aacactcggc gtcaaacggc taaagccgtg
     1981 ggtatatttc aggccaacgg cccataccac ttccagcaga ccagcaataa ctaagataat
     2041 ccaggacata tcaggctccg gaaatttggg gccgtccccg gtgaaaagag gcgtttgcag
     2101 gtcgtcctgc aaagctgatg gttgaacgga tattttgcca ggtggcaggg gaatttcaac
     2161 tattaccttt atgacgatcg cagaaaaaac aaacggcacg acactgctgt cgtaccgtta
     2221 ttgcttattg ctgcgctttc gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt
     2281 cttcaccaac gccacgcgtg gtgttgcagg cagttaatac tgttgaaagc accagaacag
     2341 aaaagatcgc tgcaattgtc ttcttcacca taacgtcttc cttttaggta atgttattta
     2401 tgtttgccta tagcaaatta ataatagaca acatcgccaa aaatgatgga aatctgatga
     2461 tttttcggaa gagaagaaaa attagctggc agcgcgagag atggagttac cgagatgttt
     2521 gatgtcttcg ccgaaaccgc aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa
     2581 aacaagaacg ataaggcgtt tcatcatctc tgacctgaat aagtgatggt gcagcctgca
     2641 ggccgcacca caaccgcatt acttcacgcg agagacgtat tcaccagagc gggtatccac
     2701 tttgatgact tcgccgattt gtacgaacag cggaacttta accacagcgc cagtagacag
     2761 ggtagccggt ttgccgccag tacctgcggt atcacctttc aggcccggat cggtatcaac
     2821 gatttccagt tcaacgaagt tcggcggagt aacggagatc ggctgaccat tccacagagt
     2881 tacgatacac tctgcctgat ccagcagcca tttagcattg tcgccaattg cttttgcatc
     2941 agcagacagc tgctcgaaag tttcgttgtt catgaagtgc cagaactcac cgtcgttgta
     3001 caggtaagtc aggttcatat cgacaacatc agcgccttca gcggaatcag tagatttgaa
     3061 ggttttttct acgcgagtac cggtcagcag acgacgcagt ttaacgcgag caaatgcctg
     3121 gcctttaccc ggttttacga attcactcgc ttcaaccgcg taaggttcgc cgtctaacat
     3181 gattttaaga ccagcacgaa aatcgttgct atagtacgtt gccataaggc cctctgaaat
     3241 ttgttaattg gtagctaagc cacaaaatgg cgcatattgt aaccctaaat accccatcca
     3301 gagaagattg gttaacgcaa cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc
     3361 ttttgaatat agacgcggac gaaaaactgt tggccggacg cagcgccaaa aagctgtttg
     3421 ccctgcgtgt gccccgctca tttatcgatc gcatggagaa aggcaatccg gacgatcctc
     3481 ttttgcgtca ggtacttacc tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg
     3541 acccactgga agaacagcac agcgtagtgc ctggtttgtt gcataaatac cacaaccggg
     3601 cgcttttgct ggtcaaaggc ggctgcgcgg taaattgccg ctattgcttc cgtcgccact
     3661 tcccctatgc cgaaaatcag ggcaacaagc gtaactggca gactgcactt gagtatgttg
     3721 ctgcgcatcc ggaactggac gagatgattt tctccggcgg cgatccgctg atggcgaaag
     3781 agcacgagct ggactggttg ctcacacaac tggaagccat cccgcatatc aaacgtctgc
     3841 ggattcacag ccgtctgccg attgtgatcc cggcacgtat caccgaggcg ctggttgaac
     3901 gctttgcccg ttctacgctg caaatcttgc tggtgaatca catcaatcat gccaatgagg
     3961 tagatgaaac gttccgtcag gcgatggcta aattgcgccg tgtcggtgtc accctgctta
     4021 accagagcgt tctgttacgt ggtgtgaacg ataacgcaca aacgttggca aacctgagta
     4081 atgcgttgtt cgatgccggc gtgatgccgt attacctgca tgtgctagat aaagtacagg
     4141 gcgcggcgca ttttatggtg agtgatgacg aagcacggca gattatgcgt gagttgctga
     4201 cattggtgtc gggatatctg gtgccgaaac tggcgcgaga aattggcggc gaacccagca
     4261 aaacgccgct ggatctccag ctacgccagc agtaactgat taaacaggga agttaaacat
     4321 taaaattgcc tgatgcgcta cgcttatcag gcctacatcg tgcattgaaa ttgaatggat
     4381 ttgcaatgaa ctgtaggccg gataaggcgt ttacgccgca tccggcaaga gcaacaaacg
     4441 caagtacagc aaccactgag aaatttaatc tttgcacgtt gcataacctt taattattca
     4501 tatgcattgc tgttgttact ttttatatag ctaacaccaa taattaacct tt
//
LOCUS       DAZOYF010000239         4517 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZOYF010000239 DAZOYF010000000
VERSION     DAZOYF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.242"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            232..1089
                     /gene="focA"
                     /locus_tag="ACHUOF_003996"
     CDS             232..1089
                     /gene="focA"
                     /locus_tag="ACHUOF_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR0612946.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1144..3426
                     /gene="pflB"
                     /locus_tag="ACHUOF_003997"
     CDS             1144..3426
                     /gene="pflB"
                     /locus_tag="ACHUOF_003997"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR0612947.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3618..4358
                     /gene="pflA"
                     /locus_tag="ACHUOF_003998"
     CDS             3618..4358
                     /gene="pflA"
                     /locus_tag="ACHUOF_003998"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR0612948.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4440..>4517)
                     /locus_tag="ACHUOF_003999"
     CDS             complement(4440..>4517)
                     /locus_tag="ACHUOF_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612949.1"
                     /translation="FDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT         1151 a         1111 c         1092 g         1163 t
ORIGIN      
        1 tgatgctccc ttttaattaa ctgttttagc ggaggatgcg gaaaaaattc aactcatttg
       61 ttaattttta aaatttattt ttatttggat aatcaaatag ttactccgta tttgcataaa
      121 aaccatgcga gttacgggcc tataagccag gcgagatatg atctatatca atttctcatc
      181 tataatgctt tgttagtatc tcgtcgccga cttaataaag agagagttag tgtgaaagct
      241 gacaaccctt ttgatctttt acttcctgct gcaatggcca aagtggccga agaggcgggt
      301 gtctataaag caacgaaaca tccgcttaag actttctatc tggcgattac cgccggtgtt
      361 ttcatctcaa tcgcattcgt cttctatatc acagcaacca ctggcacagg cacaatgccc
      421 ttcggcatgg caaaactggt tggcggcatt tgcttctctc tggggctgat tctttgtgtt
      481 gtctgcggag ccgatctctt tacttccaca gtgttgattg ttgttgctaa ggcgagtggg
      541 cgcatcacct ggtgtcagtt ggctaaaaac tggctaaatg tctattttgg caacctggtc
      601 ggcgcactgc tgtttgtact tttaatgtgg ctttccggcg agtatatgac cgcaaatggt
      661 caatggggac taaacgtcct acaaaccgcc gaccacaaag tgcaccatac ttttattgag
      721 gccgtctgtc ttggtatcct ggcaaacctg atggtatgtc tggcagtatg gatgagttat
      781 tctggccgca gcctgatgga caaagcgttc attatggtgc tgccggtcgc gatgtttgtt
      841 gccagcggtt ttgagcacag tatcgcaaac atgtttatga tcccgatggg tattgtaatc
      901 cgcgacttcg catctccgga attctggacc gctgtcggtt ctgcaccgga aaatttttct
      961 cacctgaccg tgatgaactt catcactgat aacctgatcc cggttacgat cggtaatatt
     1021 atcggcggtg gtttgttggt tgggttgaca tactgggtca tttacctgcg tgaaaacgat
     1081 caccattaat ggttgtcgaa gtacgcagta aataaaaaat ccacttaaga aggtaggtgt
     1141 tacatgtccg agcttaatga aaagttagcc acagcctggg aaggttttac caaaggtgac
     1201 tggcagaatg aagtaaacgt ccgtgacttc attcagaaaa actacactcc gtacgagggt
     1261 gacgagtcct tcctggctgg cgctactgaa gcgaccacca ccctgtggga caaagtaatg
     1321 gaaggtgtta aactggaaaa ccgcactcac gcgccagttg attttgacac cgctgttgct
     1381 tccaccatca cctctcacga cgctggctac atcaataaag cgttggaaaa agttgttggt
     1441 ctgcagactg aagctccgct gaaacgtgct cttatcccgt tcggtggtat caaaatgatc
     1501 gaaggttcct gcaaagcgta caaccgcgaa ctggacccga tgatcaaaaa aatcttcact
     1561 gaataccgta aaactcacaa ccagggcgtg ttcgacgttt acactccgga catcctgcgt
     1621 tgccgtaaat ccggtgttct gaccggtctg ccagatgctt atggccgtgg ccgtatcatc
     1681 ggtgactacc gtcgcgttgc gctgtacggt atcgactacc tgatgaaaga caaatacgct
     1741 cagttcacct ctctacaggc tgatctggaa aacggcgtaa acctggaaca gactatccgt
     1801 ctgcgcgaag aaatcgctga acagcaccgc gctctgggtc agatgaaaga aatggctgcg
     1861 aaatacggct acgacatctc tggtccggct accaacgctc aggaagctat ccagtggact
     1921 tacttcggct acctggctgc tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt
     1981 acctccacct tcctggatgt gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa
     2041 caagaagcgc aggaaatggt tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg
     2101 cgtactccgg aatacgatga actgttctct ggcgacccaa tctgggcaac cgaatctatc
     2161 ggtggtatgg gcctcgatgg tcgtaccctg gttaccaaaa acagcttccg tttcctgaac
     2221 accctgtaca ctatggggcc gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa
     2281 ctgccgctga acttcaagaa attcgccgct aaagtgtcca tcgacacctc ttctctgcag
     2341 tatgagaacg atgacctgat gcgtccggac ttcaacaacg atgactacgc tatcgcttgc
     2401 tgcgtaagcc cgatgatcgt tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg
     2461 gcgaaaacca tgctgtacgc aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt
     2521 ggtccgaagt ctgaaccgat caaaggcgat ctcctgagct acgatgaagt gatggagcgc
     2581 atggatcact tcatggactg gctggctaaa cagtacatca ctgcactgaa catcatccac
     2641 tacatgcacg acaagtacag ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt
     2701 atccgcacca tggcgtgtgg tatcgctggt ctgtccgttg ctgctgactc cctgtctgca
     2761 atcaaatatg cgaaagttaa accgattcgt gacgaagacg gtctggctat cgacttcgaa
     2821 atcgaaggcg aatacccgca gtttggtaac aatgatccgc gtgtagatga cctggctgtt
     2881 gacctggtag aacgtttcat gaagaaaatt cagaaactgc acacctaccg tgacgctatc
     2941 ccgactcagt ctgttctgac catcacttct aacgttgtgt atggtaagaa aactggtaac
     3001 accccagacg gtcgtcgtgc tggcgcgccg ttcggaccgg gtgctaaccc gatgcacggt
     3061 cgtgaccaga aaggtgctgt agcgtctctg acttccgttg ctaaactgcc gtttgcttac
     3121 gctaaagatg gtatctccta caccttctct atcgttccga acgcactggg taaagacgac
     3181 gaagttcgta agaccaacct ggctggtctg atggatggtt acttccacca cgaagcatcc
     3241 atcgaaggtg gtcagcacct gaacgttaac gtgatgaacc gtgaaatgct gctcgacgcg
     3301 atggaaaacc cggaaaaata tccgcagctg accatccgtg tatctggcta cgcagtacgt
     3361 ttcaactcgc tgactaaaga acagcagcag gacgttatta ctcgtacctt cactcaatct
     3421 atgtaattag atttgactga aatcgtacag taaaaagcgt acaataaagg ctccacgaaa
     3481 gtggggcctt ttttagcacg agagcctttt ttgtcagcta tctatacttt aaggtgactg
     3541 ccaaaacaga ctcgacgtag ccttcgagct gcgcaccaac acggcctcag atgggccaca
     3601 tctggagaaa caccgcaatg tcagttattg gtcgcattca ctcctttgaa tcctgtggaa
     3661 ccgtagacgg cccaggtatt cgctttatca cctttttcca gggctgcctg atgcgctgcc
     3721 tgtattgtca taaccgcgac acctgggaca cgcatggcgg taaagaagtt accgttgaag
     3781 atttgatgaa ggaagtggtg acctatcgcc actttatgaa cgcttccggc ggcggcgtta
     3841 ccgcatccgg cggtgaagca atcctgcaag ctgagtttgt tcgtgactgg ttccgcgcct
     3901 gtaaaaaaga aggcattcat acctgtctgg ataccaacgg ttttgttcgt cgttacgatc
     3961 cggtgattga tgaactgctg gaagtaaccg acctggtaat gctcgatctc aaacagatga
     4021 acgacgagat ccaccaaaat ctggttggag tttccaacca ccgcacgctg gagttcgcta
     4081 aatatctggc gaacaaaaat gtgaaggtgt ggatccgcta cgttgttgtc ccaggctggt
     4141 ctgacgatga cgattcggca catcgcctcg gtgaatttac ccgtgatatg ggtaacgttg
     4201 agaaaatcga acttctcccc taccacgaac tgggcaaaca caaatgggtg gcgatgggtg
     4261 aagaatacaa actcgatggt gttaaaccac cgaagaaaga gaccatggaa cgcgtgaaag
     4321 gcattcttga gcagtacggt cataaggtaa tgttctaatt tcctcgtaaa gcggcaactt
     4381 tattgagttg ccgctttttt attccgcgct tcattctgct acagcgtttt taacacatct
     4441 tatagtgcat ctaccatatc ccgtacctga gataataacg actgataata gctcgcgtga
     4501 agttcttccc catcgaa
//
LOCUS       DAZOYF010000240         4372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZOYF010000240 DAZOYF010000000
VERSION     DAZOYF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.243"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(1..473)
                     /locus_tag="ACHUOF_004000"
                     /pseudo
     CDS             complement(1..473)
                     /locus_tag="ACHUOF_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(433..654)
                     /locus_tag="ACHUOF_004001"
     CDS             complement(433..654)
                     /locus_tag="ACHUOF_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612950.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(655..1338)
                     /locus_tag="ACHUOF_004002"
     CDS             complement(655..1338)
                     /locus_tag="ACHUOF_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR0612951.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(1402..1713)
                     /locus_tag="ACHUOF_004003"
     CDS             complement(1402..1713)
                     /locus_tag="ACHUOF_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612952.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(1710..2612)
                     /locus_tag="ACHUOF_004004"
     CDS             complement(1710..2612)
                     /locus_tag="ACHUOF_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR0612953.1"
                     /translation="MYFPSEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            3052..4010
                     /locus_tag="ACHUOF_004005"
                     /pseudo
     CDS             3052..4010
                     /locus_tag="ACHUOF_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
     gene            4010..>4372
                     /locus_tag="ACHUOF_004006"
     CDS             4010..>4372
                     /locus_tag="ACHUOF_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR0612954.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQ"
BASE COUNT         1191 a         1151 c         1062 g          968 t
ORIGIN      
        1 gtgtgttgtt tttcagcagg ctgcgggcct gatcaaccat cgcctgtcgg cgggtaacgt
       61 attccgggag ggggaaaccg tgatcccggg cagcccattc acgtttccac agtgcctgtt
      121 cagcgatctg agtcagggtc tccagcagtt cagcagggac ggtcacctgg cggttttcgc
      181 gcacgttgct gataatctcc atcgccgcgg cggaagaaat accggactca atccgctagt
      241 cttccgggat gtcctccagg tgcgccagga cggtcctgat ttcatgatcg gacagggaaa
      301 tatccattcc gtcagcgagg gcctgaatct cttccgggga ccagataacc agcatcagcg
      361 gcgtgtctcc cgaatacttt gctgccagcg cccggcatag cgtttcgcat gttccgtaca
      421 tactctctcc cctcaatctt tgaagcagga atccagttcc agaatttcgg tatatgcctc
      481 catcaagagg cggtttgcgc gaaacaacgt ctgacgcacc agacgttccg gcagggtgtc
      541 agcattaaag ctgtaccgcc gctccatctc attcatggtt gtacgcaggt cgcacatggc
      601 attggccacc tccgccactt cggcgcgaag tttttcgtgt ccggcatagt tcatttacgc
      661 agcctccggc ataaaccagt catcattcgc ggccccctgc tgcatggccc gctgtacggc
      721 agccagtcgt tgctgtccgg cacggtgata ctgctcaagc agctcgatac cgatataccg
      781 gcgtccggac tggagggcgg cgacgcaggt tgaaccgctg cctgcaaacg ggtccagcac
      841 tatcgcattc gggtgtgtga agctctcaat cagcggttgc aggctggtaa cgggcttttc
      901 cgtcgggtga tggcggttgc ccgaatattt ccagcccaac acgtccggca gcgggttttg
      961 tggcagacgt ggacggcctt ttgccaggat gtaggcgcat tcgtggcgat agcccacata
     1021 tgcggccttc gatgtgtagt ttttggtgaa gaccaggtga ccaacaacgc tgaatcccgc
     1081 atttttccag gcagacataa agcgatcgac gcggttccag ccatagaagc tcaccatcag
     1141 cgcgtctttt ttgagtacgc ggtacatttc attacaggcc ggttgcagcc attcatcagt
     1201 tttatcacca gcaatggtac gcccctgacg gtcacgaaaa ccgacgagat atggcggatc
     1261 ggtgaggatg aaatcaatgg cgttatcagg aatacgggcc ataacatcga tgcagttacc
     1321 gaggacaaaa cgggacataa cagatccttc tcttacagag ggcacccctg tggtgcccgt
     1381 tccccgctgc ctgtgggctg ttcatcgctt ccggtaacgg gtcaggccga ccgtggaata
     1441 ccgcagccgt cgtctttttg acagcaaggt tatgccgcga aagcggcttg aagggcgttc
     1501 atcagaaaag ggcgacgggc tggcgtacac tgtcaccgac accagccggg agcggtaaaa
     1561 gcgcccgtcc cgtgcccgcc ggacgcggga cactgaccac aggcagcgtt tatgccgcgc
     1621 cgcgtcagcc ggcgcagtaa aaagaggagc acggcgacat cctttgtcgg aaaagaaacg
     1681 ggcggttgcc cgcccgtctc cgtcatactt catccgccat aatgcgccac attgtcgact
     1741 atccacgcag gtccggtgac ttccggctgc tcatcgtcat ctgtcgggga agaccattca
     1801 agttcccccc acagcacatt gacgagctct ccacgctcat acaactgcca gccacagaaa
     1861 tcacagccct gttcggcata ccagtgctcc agcgtgcagc tgaaacggcg gcttaattct
     1921 gcaataacgt cgctttccgg ctgacaccac ggagtgtcga aatccacctg aatgaagttt
     1981 tctccctgac tgctgatatt caggtcatat ccacaaggcc acttcacgcc ataccgttcc
     2041 gtataccagt gatatgcaga aggaacaccg ttcagaacac caccattaaa gccgttcact
     2101 tcaacatcca ggcgggtcgg cagaaccgtc agcaggtcaa acggcatggt tttttccggc
     2161 actacgttgt cacacagacg gttccaccac accgatacat cctcgttacc ccatatgtca
     2221 caccagtcac ctctttttgc ggtgaaatgc acagtgatgg tttccctggc atcatccggt
     2281 aatccttccc aacgacgctg gccagtaccg ctctgtaacc agagttcatg cagcatcagg
     2341 cagttttgtt catccagcaa cacaccgttc tgcaggtgtg tcagccagcg ggtgaacgcg
     2401 atattttccg tcgatacagc accacgtccg gcagccgtca ctccggggca ctgttcagag
     2461 cggatatttt cagtgatctg cagaagtccg gcacttccgg ccagaaacag ctgaatacct
     2521 tcatttgtgg cgcggcgata aagcggtgtg actgcaccgc tggccagtcg tttaatctca
     2581 gcgatctgtg ccggctcact aggaaaatac atacgattcg agcaccagtt tggcatggga
     2641 attctcctct cacatgcggc ctccatcagg ccgtgccgca cctgtaaccg ggcgaccgca
     2701 tacgaatgca ggtgcggccc cgaagccgca gcgcgtggag gaggaatgac gaaacgtgac
     2761 cgggtgagga ggccggcgac agcgacacac ttgcatcgga tgcagcccgg ttaacgtgcc
     2821 ggcacggcct ggttaaatag tatccgtctc agttcgtggc gcaaaatgag aaaccaataa
     2881 gaactcaaaa tgaaccacaa caagaacata aagagactct aaaagagcaa caccgtttga
     2941 catagactca aaatgagtca caataagaaa caataaaaaa ctcaaaaaga tgcaccgaca
     3001 taaaggatgc tcaatttgag ataaacacaa actcaaaacg aggcaaaaag aatgctaaaa
     3061 gtatcatgcg atgatggttc aactaacgta aaactggcct ggcttgaaga tggcgaggta
     3121 agaaccagtc tgtcaggaaa cagctttaaa gaaggctgga acccgggact gtttaatgcc
     3181 ggcaaagttt acaattacgt cgtggatgaa aaaaatacac ctatgatttg ggaagcactg
     3241 ctgtcatagg tacaacacat gtcagttatc aatacagcac caccaatctg cttgccatac
     3301 atcatgcact gctgacatcc ggccttcagc cacaggatgt agaactgaca gtaacattac
     3361 ctgtgaccga attctttgat aatgacaatc aaccaaatga agaaagaata gaaagaaaaa
     3421 aggcaaatgt tctccgtgag atatctctga ataaagggga aacatttaaa attaaaaaag
     3481 ttaatgtaat gcctgaatct ttgccagctg catttgaatc actaaaaaaa gataaagtaa
     3541 acaaacttga acgcagcctg attatcgatc ttggaggaac cactctggat tgtggtttaa
     3601 ttctcggtgc atttgaaggc atttctgaaa tccgtggcta ttcagaaatc gggacatccc
     3661 gcatcactca taccgtcatg aatgcgctga cgaaagcctc cacaccatgt aattatttta
     3721 ttgctgatga gttaataaaa aacagacacg ataatgaata tctccagaca ttaataaatg
     3781 atgtagctga aataaaaaac atttctcatg taatagacag agaagtgaaa tctctggcag
     3841 aaagcatcag gcaggaaatt tcaacattct caggaatgaa ccgaatctat ctcactggcg
     3901 gaggagccga gcttatttac cctcacataa agcaatattt cccgaatctg aaagtgaaca
     3961 aggtggatga acctcagttt gcattggtga aagcaatggt gcatgcgtaa tggaaagcag
     4021 tgatccaaaa aaaagaaaga aagttgtggc atacctgcac ccggcacttt atccgcagga
     4081 caacctgaca caacaaacaa tagactctct cccagtacaa atgcggggag atttctacag
     4141 acaatccctt atttgcggag cagctcttta ttctgttgcc ccccgactgt taacgctaat
     4201 cagcgttttt ttcagtgaga aaatcactgc agaaaatctg gtgaaactta tagaacaaac
     4261 aacaggatac acatccacat caattgatat aagtgtgtta aaaaacatca tagaggcatc
     4321 ttcagaaaac aaatcagaga gcatcacttc aaaagatgat ttcgaagaac aa
//
LOCUS       DAZOYF010000241         4349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZOYF010000241 DAZOYF010000000
VERSION     DAZOYF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.244"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            339..1397
                     /gene="rfaQ"
                     /locus_tag="ACHUOF_004007"
     CDS             339..1397
                     /gene="rfaQ"
                     /locus_tag="ACHUOF_004007"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR0612955.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1394..2518
                     /locus_tag="ACHUOF_004008"
     CDS             1394..2518
                     /locus_tag="ACHUOF_004008"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR0612956.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2511..3308
                     /gene="rfaP"
                     /locus_tag="ACHUOF_004009"
     CDS             2511..3308
                     /gene="rfaP"
                     /locus_tag="ACHUOF_004009"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR0612957.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            3351..>4349
                     /gene="waaO"
                     /locus_tag="ACHUOF_004010"
                     /gene_synonym="rfaI"
     CDS             3351..>4349
                     /gene="waaO"
                     /locus_tag="ACHUOF_004010"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFR0612958.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHIFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEK"
BASE COUNT         1234 a          874 c          989 g         1252 t
ORIGIN      
        1 tttgacttaa atagcaattt acccgccaat tcttgttgaa catctatact ttttttgtat
       61 ttttatgcga ttcattgatt tctgctgtac gtgcaggaag gaatcgcaga gcaacagtga
      121 aagaatatgg ttgctaatga ttttatttga ttgatttctt tattgaaaat caacaatgtg
      181 cttaaaaatc attcgggtcg gattgccgcg aaagaagtga tacactagcg cgtcaatatc
      241 agtgcggtat cagtattcag gtagctgttg agcctggggc ggtagcgtgc tttttctgct
      301 tcaccttaat cggataatct caacaaaaag agttacttgt ggataagcca tttcgaagaa
      361 ttttgctcat taagatgcgt tttcatgggg atatgttatt aactactccc gtcattagtt
      421 ctctgaaaaa aaattaccct gacgcaaaaa tcgatgtgct gctttatcag gacaccatcc
      481 cgatcctgtc tgaaaatcca gagattaacg cgctctacgg cataaaaaat aaaaaagcaa
      541 aagcctcaga aaaaattgcc aacttttttc atctcatcaa ggtattacgt gccaataagt
      601 atgaccttat cgtcaatctt accgatcaat ggatggttgc tatactggtt cgcttattaa
      661 atgctcgtgt gaaaatttcc caggattatc atcatcggca gtctgctttt tggcgtaaaa
      721 gtttcaccca tttggtgccg ttgcagggtg gaaatgtggt ggaaagtaac ttatccgtgc
      781 tgacaccatt gggacttgat tcgttggtga agcagacaac catgagttac ccgcctgcaa
      841 gctggaaacg tatgcgtcgc gaacttgatc acgctggtgt tggacaaaat tatgtggtta
      901 tccaacctac ggcgcggcaa atcttcaaat gctgggacaa cgccaagttt tccgctgtga
      961 ttgatgcctt acatgctcgt ggttatgaag tcgttctgac gtccggccca gataaagacg
     1021 atctggcctg cgtcaatgaa attgcgcagg gatgccagac gccaccagta acggcgctgg
     1081 ctggaaaggt gaccttcccg gaacttggtg cgttaatcga tcatgcgcag ctgtttattg
     1141 gcgttgattc cgcaccggcg catattgccg ctgcagttaa tacgccgctg atatcgctgt
     1201 ttggcgcaac agatcatatt ttctggcgtc cctggtcaaa taacatgatt caattctggg
     1261 cgggagatta ccgggaaatg ccaacgcgcg atcagcgtga ccgaaatgag atgtatcttt
     1321 cggttattcc ggcggcagat gtcattgctg ctgtcgataa attactgccc tcctccacga
     1381 caggtacgtc gttatgatcg ttgctttttg tttatataaa tattttccct ttggcggttt
     1441 gcagcgcgat tttatgcgta ttgctcagac agtcgccgcc cgaggtcatc atgttcgggt
     1501 ttatacccag tcgtgggaag gcgaatgccc tgatgtattt gaagtgatca aagtgccggt
     1561 taaatcgcat accaatcacg ggcgcaatgc ggagtatttt gcctgggtgc aaaaacattt
     1621 acgcgaacat cccgtcgata aagtcgttgg attcaacaaa atgccggggc tggacgttta
     1681 ttatgccgct gatgtttgtt atgccgagaa agtagcgcag gaaaaaggct ttttctatcg
     1741 cctgacgtca cgttatcgcc attatgccgc ctttgagcgg gcaaccttcg aacagggcaa
     1801 gccgacacag ctgctgatgc tgacagataa gcaaatcgcc gatttccaga aacattatca
     1861 gactgaagcg gagcgttttc atattctgcc accggggatt tatcctgatc gtaaatatag
     1921 ccagcagcca gccaatagcc gtgaaatctt ccgtaagaag aatggaataa ccgaacaaca
     1981 atatttattg ttgcaggtcg gttcagactt cacgcgtaaa ggtgccgatc gttccattga
     2041 agcacttgct tcgttaccgg attcgctgcg ccacaacaca ttgctatatg ttgttgggca
     2101 ggataaaccg cgaaaatttg aggcactggc agaaaaacgc ggcgtgcgca gtaatgttca
     2161 cttcttctcg gggcgcaacg atgtctcgga attaatggcg gcggcggatt tattactgca
     2221 tcctgcctac caggaagcgg cgggaattgt gctgctggaa gcgattactg caggattacc
     2281 ggtactaaca actgccgttt gtggctatgc gcattatatt gtcgacgcta attgcggcga
     2341 ggctattgct gagccattcc gccaggaaac attgaatgag attttacgca aagcgttaac
     2401 gcaatcttca ttgcgccagg cttgggcgga aaatgcgcga cattatgctg atacacaaga
     2461 tttatacagt ctgccagaga aagcggcgga tatcataacg ggtggtctgg atggttgaac
     2521 ttaaagagcc gtttgccacg ttatggcgcg gcaaagatcc ttttgaggaa gttaaaacct
     2581 tgcagggtga ggtatttcgt gaactggaaa ctcgccgtac tctgcgcttt gaaatggcgg
     2641 gaaaaagcta ttttctcaaa tggcatcgcg gcacgaccct gaaagagata atcaaaaatt
     2701 tactctcatt gcggatgcca gtattaggcg cagaccgcga atggaatgcg attcatcgac
     2761 tgcgggatgt cggcgttgat actatgtatg gggtggcatt cggcgaaaaa ggcatgaatc
     2821 cgctgaccag aacctcgttt attattaccg aagatctgac accaaccatc agtctggaag
     2881 attactgtgc tgactgggcg accaacccac cagatgttcg cgtaaagcgt atgcttatta
     2941 agcgtgtcgc gacgatggtg cgcgatatgc atgctgcggg cattaaccat cgtgactgtt
     3001 atatctgtca tttcctgctg cacttgcctt tttccggtaa ggaagaggag ttaaaaattt
     3061 cggtaattga cctacaccgg gcgcagcttc gcacgcgcgt tccaggtcgt tggcgcgata
     3121 aagatcttat agggctttat ttttcttcaa tgaatattgg cctgactcag cgggatatct
     3181 ggcgctttat gaaggtgtat tttgccgccc cgcttaaaga cattctcaag caggaacaag
     3241 gactgctgtc gcaagcagaa gcaaaagcca caaaaatcag ggaaagaacg attcgaaaat
     3301 cgttgtaacc cgtaacttat ttttgccaaa attttggata cagaataaat atgtctcaac
     3361 tcaatgatag tgacatcatc ctttttgagt ataattttca ttatcaaaat ataagatcta
     3421 aaaatactct tgatatagca tttggtattg acagaaattt tctttttgga tgtggtgtag
     3481 ccatcgcatc tattctatta aacaatagag aaatctcttg tgaatttcat attttcacag
     3541 attatatcag tgataaagac aaattatatt tttctgattt agcaaaacaa tataattcaa
     3601 gaattaatat ttatgttatc aattgtgata agctgaagtc attaccaagc acgaaaaact
     3661 ggacttacgc aacatatttt cgatttataa ttgcagatta tttctatcat aaacatgaaa
     3721 aaatactata tcttgatgca gatattgctt gcaagggtag tattaaagaa ctcttagatt
     3781 atcaattttc tactaatgaa attgccgcag ttgtagctga aagagatatt gaatggtggc
     3841 aaaatcgagc ctcggtatta actacaccac agttagcttc tggatatttc aatgctggtt
     3901 ttttactgat aaatattgat gagtggaatc taaataacat ttcgtcaaaa gctattgaaa
     3961 tgttgcgtga cccagattgg gtaagtaaaa tcacccacct tgatcaagat gtactgaatg
     4021 tattattgaa tggtaaagtg aagtttattt cggagaaata taatacccga tatagtatta
     4081 actatgaatt aaaagacaaa gttgataatc cagtcaatga tgacaccgtg tttatacact
     4141 atgtgggacc tacaaaaccc tggcatgagt gggctgatta tccggtttca cgtagttttt
     4201 tgatcgccaa agcagcttct ccgtggagta aagaagattt acttaaacct gtaaatagca
     4261 atcagtatcg gtattgtgca aaacataaat ttaaacaaaa gcattatatg gcaggcattt
     4321 ttaattattt aaagtattat aaagaaaaa
//
LOCUS       DAZOYF010000242         4260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZOYF010000242 DAZOYF010000000
VERSION     DAZOYF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.248"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            188..1252
                     /gene="ulaG"
                     /locus_tag="ACHUOF_004011"
     CDS             188..1252
                     /gene="ulaG"
                     /locus_tag="ACHUOF_004011"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR0612959.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1360..2115
                     /gene="ulaR"
                     /locus_tag="ACHUOF_004012"
     CDS             1360..2115
                     /gene="ulaR"
                     /locus_tag="ACHUOF_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR0612960.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2112..2861)
                     /gene="yjfP"
                     /locus_tag="ACHUOF_004013"
     CDS             complement(2112..2861)
                     /gene="yjfP"
                     /locus_tag="ACHUOF_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0612961.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3043..3372
                     /gene="bsmA"
                     /locus_tag="ACHUOF_004014"
     CDS             3043..3372
                     /gene="bsmA"
                     /locus_tag="ACHUOF_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR0612962.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3521..3796
                     /gene="yjfN"
                     /locus_tag="ACHUOF_004015"
     CDS             3521..3796
                     /gene="yjfN"
                     /locus_tag="ACHUOF_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR0612963.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3913..>4260)
                     /locus_tag="ACHUOF_004016"
     CDS             complement(3913..>4260)
                     /locus_tag="ACHUOF_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR0612964.1"
                     /translation="SGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRL
                     QQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQ
                     NDLLLRATGGVCV"
BASE COUNT         1138 a         1061 c         1061 g         1000 t
ORIGIN      
        1 tttatttaaa ttcatttagt taatttaact acacattcca ttaaatcttt ccccactcaa
       61 aatcaacaac aacttccctg gcaaaaagtc aaaaaatggt cattaccttc ctgaaggaat
      121 agtgtcagga ttaatcaaaa ccaatcacat gttgattcga attgaactat catgaaggta
      181 aatggcgatg agtaaagtga aaagtatcac ccgagaatcc tggatcctga gcactttccc
      241 ggagtggggt agctggctga atgaagaaat tgaacaagag caggtcgccc ctggcacatt
      301 tgcgatgtgg tggctaggct gcaccgggat ctggttgaaa tcagaaggtg gcaccaacgt
      361 ttgcgttgat ttctggtgcg gcactggcaa acaaagtcac ggtaacccgt taatgaaaca
      421 gggtcaccag atgcagcgca tggctggcgt gaaaaaactg cagccaaacc tacgcaccac
      481 cccgtttgtt cttgatccgt ttgcgattcg ccagatcgac gcggtactgg cgactcacga
      541 tcacaacgat catatcgacg ttaacgtcgc tgctgccgtg atgcagaact gtgctgatga
      601 cgtaccattc attggaccga aaacctgcgt tgatttgtgg attggctggg gcgtaccgaa
      661 agagcgttgc atcgtggtca aaccgggcga tgttgtaaaa gtgaaagaca ttgaaattca
      721 tgcgcttgac gctttcgacc gtactgcact gatcaccctg cctgccgatc aaaaagcggc
      781 tggcgtactg ccagatggca tggacgatcg cgcagtaaac tacctgttca aaacccctgg
      841 cggttcccta tatcacagtg gcgattccca ctactctaac tattatgcga agcacggtaa
      901 cgaacatcag atcgatgtgg cgttaggatc gtatggtgag aacccgcgcg gtattaccga
      961 caaaatgacc agcgccgata tgctgcgtat gggtgaagcc ctgaatgcga aagtggttat
     1021 cccattccac cacgacatct ggtcaaactt ccaggccgat ccgcaagaga tccgcgtgct
     1081 gtgggagatc aaaaaagatc gtctgaagta tggcttcaag ccgtttatct ggcaggttgg
     1141 cggcaaattt acctggccgc tggataaaga caacttcgag taccactatc cgcgcggttt
     1201 cgatgattgc ttcactattg aaccggatct gccgttcaag tcattcctgt aatctgtcgt
     1261 ttataccgga tagcgtggag aagttcgcgc tatccggcgt agtatttcct ggctatttca
     1321 aatatcatct aaaaaaatca aattttatcg gaatagctca tgactgaagc acaaagacat
     1381 caaatcctcc tggaaatgct cgcacaattg ggctttgtga ccgttgagaa agtcgttgag
     1441 cgtctgggaa tttcgcctgc cactgcgcga cgcgatatca ataaacttgg cgaaagcggc
     1501 aaactgaaaa aggtgcgcaa tggcgcagaa gctattaccc aacagcgccc gcgctggacg
     1561 ccgatgaatc tgcatcaggc gcagaatcac gatgaaaaag tacgtattgc taaagcggcc
     1621 tcgcagctgg ttaatccggg cgaaagcgta gtcatcaact gcggctccac cgcgtttctg
     1681 cttgggcggg aaatgtgtgg caagccagtg caaatcatca ctaattatct accgctggca
     1741 aattacctga tcgatcaaga acatgacagc gtgatcatta tgggtggaca gtacaacaaa
     1801 agtcagtcca tcactttaag cccgcagggc agcgaaaaca gtctctatgc cgggcactgg
     1861 atgtttacca gcggaaaagg gctgaccgca gaagggttgt ataaaaccga tatgctgaca
     1921 gcaatggcag agcagaagat gctgagcgtg gtagggaaac tggtggtact ggttgatagc
     1981 agtaagattg gcgaacgcgc gggaatgctt tttagccgtg ccgatcaaat cgatatgctg
     2041 atcaccggca aaaatgctaa cccggaaatc ctgcaacaac tggaagcgca aggggtcagc
     2101 attctgcgtg tttaaagatg ctggcggaaa aatgtcacgg cagcatctaa cgcctcaggc
     2161 gtaatgcggt gacgcacgcc tggctgccat gaacaggtta gcagtttatc ccgtcccgtc
     2221 tcgcttaagg cctgctgcaa acgtagtgat tcgtcggcag gcacaacgtc atcgtcgagg
     2281 ccatgccaca gcagtagagg tctgtcccca agttgttcca ggtggtttgt cgcttcccac
     2341 tctgccagcg gcgcgacaat tttattgaat tcattctgct gtgctgccgt ttcaggtatc
     2401 agcggtggaa acagtgaacg ggcgagcgat gtaaaatagc ccgatcccat catgctggcg
     2461 gtacatttca ccgtggggtg gcgagcggta attcccagtg ccgtcatcgc gcccatcgac
     2521 gcgccaccga ctgccagacg gtcatcaagc agccagtttt cttcggctat tgccgcacgt
     2581 aaagtagtga attcctgcat actttgtagc aagatttgcc agaattgatt taaccgccgc
     2641 gcttcgtcgc cactaaaacg gctaccgtga tcgggcgcat ccggcatgat cacccgcaaa
     2701 ccagcctgcg ccagcgcaac ggcaaaatag ctatacacca ggctggatga agtaaagccg
     2761 tgataaaaaa ttacgcacgg taacggggta tctttttgcc cgacaggata agcatgaaga
     2821 acgggaatat ctgccagctc gcgtgattct atttcaatca tctgtctctc ctttttgtct
     2881 ggtatgacat gaagaaatcc ttagcacaag tttctgctgg cggtattagt tcataatgtt
     2941 gagatgtggg ttacgctttc gtcacgtttt cattcgaaaa tcgcgtacca ggtaacaatt
     3001 cgggaacatt cccccaaagt taaattaata aggcactaca ctatggttag caggaaacgt
     3061 aatagcgtta tttaccggtt tgccagttta ttattggtgt tgatgttaag tgcctgtagc
     3121 gcactgcaag gtacgccaca gccagcaccg ccagtaacgg accatccgca agagattcgc
     3181 cgcgaccaga cgcaagggtt acagcgaata ggtagcgtaa gcacgatggt tcggggttct
     3241 ccggatgacg cattagcaga aatcagagca aaagctgtcg ctgcaaaagc tgattattac
     3301 gttgtcgtca tggtggacga aaccatcgtg acaggacagt ggtattcaca agccattttg
     3361 taccgtaaat aaatcagaca acttacatag tcagctttac attgctttgc gtccctatgc
     3421 gtttacctgt gcacaataaa ttaccggctg gaagcttggg cgtttattac cgcgatggaa
     3481 tgccctgcaa catgtgggga aacgaaaaat ggagctgacg atgaaacaat tacttgcctc
     3541 accctcgctg caattagtga cttatcctgc gagcgccacg gcacagtctg cagaattcgc
     3601 cagtgctgac tgcgtgacgg gcctgaatga aataggtcag atctcagtta gcaatatttc
     3661 aggggatccg caggacgtgg aacgtatcgt ggcgttaaag gccgatgaac aaggtgcgtc
     3721 atggtaccgc attattacca tgtacgaaga ccagcagcct gataactggc gcgtacaggc
     3781 gatactgtat gcatgagtgt catctacagc aaatgaacaa tgccggatgc ggcgcgagcg
     3841 ccttgtccgg cctacaattt tcacatattt catttagtta atcgaaacca gcgtcgcatc
     3901 agtcgtatac gcttacacac acactccccc cgtcgcccgc agcaataaat cattctggat
     3961 ctgctctgac aaccgtacgc cgccgcgcgt atctaacatc acctgacacc acgcctgcgc
     4021 cattggcgga gaagcatatt tcaacatttg cgcaccgcag cccagcagga atagctgatg
     4081 agtaatctct cgccccagtt cttcagctgg cttacgcagc tgctgctgta aacgacgaac
     4141 cgcgcgatca aaatagcgat cctgcccttt cacttccaca aatgcttccg acaataagtc
     4201 gtatacgccc gcttgcttat tgagaacgcg caacacatcc aggcacataa tattgccgga
//
LOCUS       DAZOYF010000243         4219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZOYF010000243 DAZOYF010000000
VERSION     DAZOYF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.249"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(28..2004)
                     /gene="speA"
                     /locus_tag="ACHUOF_004017"
     CDS             complement(28..2004)
                     /gene="speA"
                     /locus_tag="ACHUOF_004017"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR0612965.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2013..2144)
                     /gene="yqgB"
                     /locus_tag="ACHUOF_004018"
     CDS             complement(2013..2144)
                     /gene="yqgB"
                     /locus_tag="ACHUOF_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR0612966.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            complement(2238..2381)
                     /gene="yqgG"
                     /locus_tag="ACHUOF_004019"
     CDS             complement(2238..2381)
                     /gene="yqgG"
                     /locus_tag="ACHUOF_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR0612967.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2492..2743)
                     /gene="yqgD"
                     /locus_tag="ACHUOF_004020"
     CDS             complement(2492..2743)
                     /gene="yqgD"
                     /locus_tag="ACHUOF_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR0612968.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2799..3953
                     /gene="metK"
                     /locus_tag="ACHUOF_004021"
     CDS             2799..3953
                     /gene="metK"
                     /locus_tag="ACHUOF_004021"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR0612969.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1067 a         1141 c         1017 g          994 t
ORIGIN      
        1 atcgacccgc cagcggaata atcttaatta ttcatcttca agataagtat aaccgtacaa
       61 acctgcctcg aactcttcaa ggaactgttg ttgcagttca gcatcaagat cggttttctt
      121 cacttgatcg cggaactggg ttaacagcgt tttcggatcg agctgtacat attgcagcat
      181 gtccgccacg gtatcgcctt cgtcagacag ttctacttct acgctaccgt cagggaacac
      241 gaacacgtca actgcttcgg tatcaccgaa caggttgtgc atattgccaa ggatctcctg
      301 atatgcaccg accataaaga aaccgagcat cggcggattc tctggatcgt actccggcat
      361 tggcattgtc gtggcaatac catcaccatc aatatagtgg tcgatagcac cgtcagagtc
      421 acaggtaata tccagcagca cagcgcgacg ttccggcact tgatccagcc cttccagcgg
      481 tagaaccggg aacaactggt cgatccccca tgcgtccggc attgactgga acagcgagaa
      541 attgacgtac attttgtccg ccatacgttc ctgcagttcg tcgattatcg gacgatgcgc
      601 acggttttgc ggatccagtt gcttctgcac ttcatggcac atgctcagat agagttgttc
      661 tgcccatgca cgttcttgca ggctaaaggt gccggaagag tagccgatat gaatgtcgtg
      721 cagatccatc tggctgtcgt gtaaccattc acgcaaagaa cggcgagttc ccggttcgtg
      781 catttcctgc caggtttccc acatgctttg cagcgcacgc ggcgcatctt ctacgggcgc
      841 ggtcggcacc gtatattcgt tacgttccac gccgatgata ttcgacacca gcacggtatg
      901 gtgcgcagtc accgcacggc ccgattcggt gattaccgtc ggatgcggca gaccattttc
      961 ttcacacgca tcgccaatcg cccagatgat gttgttggcg tattcattga ggccatagtt
     1021 tacggagcag tcagactgcg aacgggtgcc ttcataatcc acgcccagac cgccgccgac
     1081 gtcgaagcac tgaatattga cgcccagctt gtgcagttcc acatagaaac gcgcggattc
     1141 acgaacgcct gtcgcgatat cgcgaatatt cgccatctgc gaaccgaggt ggaagtgcag
     1201 tagttgcagg ctgtcgagac gcccggcttc acgcagggtt tcaaccagtt gcagtacctg
     1261 agtcgcagcc aggccgaact tcgatttttc cccgccggag gactgccatt tacccgagcc
     1321 ctgcgaagcc agacgtgcac gcacgcccag acgaggaacg acattcagac gttctgcttc
     1381 atccagcaca atggcgattt ctgacatctt ctcaatgacc agatagacct tgtgccccat
     1441 cttctcgcca attaatgcca ggcggatata ttcgcggtct ttataaccgt tgcagacgat
     1501 gacgctacga gtcatgccag catgtgccag cactgccatc aactcggctt tggaaccggc
     1561 ttccagaccc agcggttcac ccgaatgaat cagggactcg atcacgcggc ggtgctggtt
     1621 aactttgatc ggataaacaa ggaagtaatc gccgttatag ccgtaggatt ccctcgcacg
     1681 tttgaacgcg gcgttaatgg aacgcaaacg gtgctgcagg atctgtggga aacagaacag
     1741 tgcaggcaga cgctgaccct gtgcttcacg agttttcact aactgcgcga gatcgacgcg
     1801 agcttccggg acgtccgggt ccgggcacac gctgatgtgg cccagctcgt taacgtcata
     1861 gtagttattg ccccaccagg caatattgta agtacgcagc atcttgctgg cttcctggga
     1921 gctcattgca acctcctgca tggagcgtag tacaccgtgt tcgcccgctg acgaaggcaa
     1981 acccatagac atgtcgtcag acatagcgaa cctcaaatta ttttattaag tgtaaaacag
     2041 ttaacgactg tcgcagcctg gaactgcgat aacaacccat aagcacatgg attttccagc
     2101 agtgtatgct gacgctccga ctgcgcaacc ggtttctttt tcataacatt attaagcaca
     2161 taaccgaacg taagtgtgac agttcggcga aaccacgaga aaactcttgt ttttacaaga
     2221 gcgcccttgt tcagtcctca gtaactgtaa ccagctcttg aatcctgaga agcgccgaga
     2281 tgggtataac atcggcaggt atgcaaagca gagatgcaga gtgcggggaa cgagtcttca
     2341 ccagaacggt gagacaggtt aagcagcaga caacggttca ttatttcgta tcacctccac
     2401 ggccgcctgt taagacgaac ccacaagcca aaactctgat ttcaacccgg ctggaagtgg
     2461 caacacgaaa gaaacgtcgt gtgcttttta tttaagccgc gcgccgcgtt ttatacccca
     2521 caatggcaga aaattgcaaa agataaatac gcagaatgcc ggcattgtca ggaaaaattt
     2581 ccagccacgt ttttaacaga atgagacacg attcaaaaaa aagtggaaat cgggtgaaga
     2641 attgacctaa aatagccatc cagatgttaa tccatccata ccgattaaca ctcagactgc
     2701 cagtgttttt aacctgcaga gtcgtggtag gatccgctac cacagaaaat ccacacaaca
     2761 gtttgagcta accaaattct ctttaggtga tattaaatat ggcaaaacac ctttttacgt
     2821 ccgagtccgt ctctgaaggg catcctgaca aaattgctga ccaaatctct gatgccgttt
     2881 tagacgcgat cctcgaacag gatccgaaag cacgcgttgc ttgcgaaacc tacgtaaaaa
     2941 ccggcatggt tttagttggc ggcgaaatca ccaccagcgc ctgggtagac atcgaagaga
     3001 tcacccgtaa caccgttcgc gaaattggct atgtgcattc cgacatgggc tttgacgcta
     3061 actcctgtgc ggttctgagc gctatcggca aacagtctcc tgacatcaac caaggcgttg
     3121 accgtgccga tccgctggaa cagggcgcgg gtgaccaggg tctgatgttt ggctacgcaa
     3181 ctaatgaaac cgacgtgctg atgccagcac ctatcaccta tgcacaccgt ctggtacagc
     3241 gtcaggctga agtgcgtaaa aacggcactc tgccgtggct gcgcccggac gcgaaaagcc
     3301 aggtgacttt ccagtatgac gacggcaaaa tcgttggtat tgatgctgtc gtgctttcca
     3361 ctcagcactc tgaagagatc gaccagaaat cgctgcaaga agcggtaatg gaagagatca
     3421 tcaagccaat tctgcccgct gaatggctga cttctgccac caaattcttc atcaacccga
     3481 ccggtcgttt cgttatcggt ggcccaatgg gtgactgcgg tctgactggc cgtaaaatta
     3541 tcgttgatac ctacggcggc atggcgcgtc acggtggcgg tgcattctct ggtaaagatc
     3601 catcaaaagt ggaccgttcc gcagcctacg cagcacgtta tgtcgcgaaa aacatcgttg
     3661 ctgctggcct ggccgatcgt tgtgaaattc aggtttccta cgcaatcggc gtggctgaac
     3721 cgacctccat catggtagaa actttcggta ctgagaaagt gccttctgaa caactgactc
     3781 tgctggtacg tgagttcttc gacctgcgcc catacggact gattcagatg ctggatctgc
     3841 tgcacccgat ctacaaagaa accgcagcat acggtcactt tggtcgtgaa catttcccgt
     3901 gggaaaaaac cgacaaagcg cagctgctgc gcgatgctgc cggtctgaag taatctttct
     3961 tcacctgcgt tcaaaggcca gcctcgcgct ggcctttttc ttttacctca ccgcggtgga
     4021 taggcgttca cgccgcatcc ggcaaaaaaa ctgcccgcac aataacatca ttcttcctga
     4081 tcacgtttca cggcaaatta ccatcacaac tgaaaccgat tacaccaacc acaccagcaa
     4141 aatatttgta atattttcat attattattc agctttcaca gttgttacat ttcttttcag
     4201 taaagtctta attgcagat
//
LOCUS       DAZOYF010000244         4209 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZOYF010000244 DAZOYF010000000
VERSION     DAZOYF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.250"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHUOF_004022"
     CDS             complement(<1..209)
                     /locus_tag="ACHUOF_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0612970.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHUOF_004023"
     tRNA            complement(324..399)
                     /locus_tag="ACHUOF_004023"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHUOF_004024"
     tRNA            complement(406..480)
                     /locus_tag="ACHUOF_004024"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHUOF_004025"
     tRNA            complement(597..681)
                     /locus_tag="ACHUOF_004025"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHUOF_004026"
     tRNA            complement(690..765)
                     /locus_tag="ACHUOF_004026"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHUOF_004027"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHUOF_004027"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR0612971.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHUOF_004028"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHUOF_004028"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR0612972.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHUOF_004029"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHUOF_004029"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR0612973.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAZOYF010000245         4198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZOYF010000245 DAZOYF010000000
VERSION     DAZOYF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.251"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            84..752
                     /gene="ykgH"
                     /locus_tag="ACHUOF_004030"
     CDS             84..752
                     /gene="ykgH"
                     /locus_tag="ACHUOF_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR0612974.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="ACHUOF_004031"
     CDS             complement(995..1690)
                     /locus_tag="ACHUOF_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR0612975.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="ACHUOF_004032"
     CDS             complement(1683..3110)
                     /locus_tag="ACHUOF_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR0612976.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="ACHUOF_004033"
     CDS             complement(3121..3840)
                     /locus_tag="ACHUOF_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR0612977.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DAZOYF010000246         4185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZOYF010000246 DAZOYF010000000
VERSION     DAZOYF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.252"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..192
                     /locus_tag="ACHUOF_004034"
     CDS             <1..192
                     /locus_tag="ACHUOF_004034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0612978.1"
                     /translation="SIQSNRDWLSMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGIT
                     NIISIILQQAQDYDVAKIT"
     gene            complement(350..997)
                     /gene="ychE"
                     /locus_tag="ACHUOF_004035"
     CDS             complement(350..997)
                     /gene="ychE"
                     /locus_tag="ACHUOF_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR0612979.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(1028..1081)
                     /gene="ychE"
                     /locus_tag="ACHUOF_004036"
     CDS             complement(1028..1081)
                     /gene="ychE"
                     /locus_tag="ACHUOF_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFR0612980.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1474..4149
                     /gene="adhE"
                     /locus_tag="ACHUOF_004037"
                     /gene_synonym="adhC"
     CDS             1474..4149
                     /gene="adhE"
                     /locus_tag="ACHUOF_004037"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR0612981.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1022 c          960 g         1035 t
ORIGIN      
        1 tcaattcaat caaatagaga ttggctaagc atgtctatct tttctgatta cagttccagt
       61 agtgaaatgc acaataattt aacaattgat tattatttag cactttcctc taccaaaggc
      121 agtggaatta ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg
      181 aaaataacat gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg
      241 agaagaatat ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa
      301 taatgacgaa gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca
      361 ggccggggaa aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca
      421 atagccccat aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc
      481 acggtgccat gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga
      541 aaccaaacag atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg
      601 cccctggccc cgccatcaac ggcaacgcca atggcaccac accaatactt tcacgtaccg
      661 cggtttctga tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg
      721 acatcgctat cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac
      781 caaaaagttg tagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag
      841 acaggttggc tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc
      901 tgataaagac gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt
      961 tgaagtaaac gggaaaatca aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg
     1021 gataatgtta gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca
     1081 tttttcacct cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa
     1141 aacatgctaa tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg
     1201 aaaagcgatg ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta
     1261 cccagaagtg agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc
     1321 taatgtttaa actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt
     1381 tcatcggtta agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt
     1441 actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt
     1501 aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag
     1561 caagtagaca aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc
     1621 gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac
     1681 cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg
     1741 tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt
     1801 atcgttccga ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag
     1861 acccgtaacg ccattatctt ctccccgcac ccgcgtgcaa aagatgcaac caacaaagcg
     1921 gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg
     1981 atcgatcaac cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg
     2041 atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct
     2101 atcggtgtag gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt
     2161 gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa
     2221 cagtctgttg ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac
     2281 ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaat
     2341 ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc
     2401 ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc
     2461 gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc
     2521 gaagacgcgg tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct
     2581 tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg
     2641 aaaacagctc gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat
     2701 aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc
     2761 tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa
     2821 aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc
     2881 gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc
     2941 ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa
     3001 actgaagtct tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca
     3061 gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg
     3121 gacgccgcga agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg
     3181 gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa
     3241 gcgaaaatga tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg
     3301 gttgtaactg acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg
     3361 gatatggcga ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc
     3421 ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag
     3481 ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc
     3541 taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc
     3601 gcgggtatcg catttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg
     3661 ggttcccagt tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt
     3721 cgctacaatg cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg
     3781 caggctcgcc gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac
     3841 cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg
     3901 ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg
     3961 gataaactgt ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg
     4021 ctgatctccg agctgaaaca gattctgctg gatacctact acggtcgtga ttatgtagaa
     4081 gatgaaactg cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa
     4141 tccgcttaat cagtagcgct gtctggcaat ataaacggcc ccttc
//
LOCUS       DAZOYF010000247         4182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZOYF010000247 DAZOYF010000000
VERSION     DAZOYF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.253"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..186)
                     /locus_tag="ACHUOF_004038"
     CDS             complement(<1..186)
                     /locus_tag="ACHUOF_004038"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR0612982.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHUOF_004039"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHUOF_004039"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR0612983.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="ACHUOF_004040"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="ACHUOF_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR0612984.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="ACHUOF_004041"
     CDS             3863..4144
                     /locus_tag="ACHUOF_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR0612985.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1043 c         1062 g         1061 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagt
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAZOYF010000248         4177 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZOYF010000248 DAZOYF010000000
VERSION     DAZOYF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.254"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(51..3101)
                     /gene="fdnG"
                     /locus_tag="ACHUOF_004042"
     CDS             complement(51..3101)
                     /gene="fdnG"
                     /locus_tag="ACHUOF_004042"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2514..2516),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0612986.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            3295..4128
                     /gene="fdhD"
                     /locus_tag="ACHUOF_004043"
     CDS             3295..4128
                     /gene="fdhD"
                     /locus_tag="ACHUOF_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR0612987.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHVAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT          894 a         1089 c         1155 g         1039 t
ORIGIN      
        1 gcggaacgac gaatgatatc ttgagattga taagccatat gtcgtctccg ttacaccttt
       61 tccacattca caaggaagga cttaaactcc ggcgtctgcg tgttcgcatc accaacgaat
      121 ggcgttaaag tattggcaat aaagcctttt ttagctacgc cttcatagcc ccagtgaatc
      181 ggaataccga tggtatcgat atctttgccg ttcgctttca gcgtgcgaat acgtttggtc
      241 accaccgctt tggctttgat atagccacgg ttggaggaga ctttcacggt atcgccctgg
      301 gcaatgccaa gtttattcgc cagcgactcc ccgatttcca caaactgctc tggctgcaaa
      361 atcgcgttca acagcgcgtg tttggtccag tagtggaagt gctcggtcag acgataggtg
      421 gttccgacat acgggaactt atcggcttta cccaatgctt cggcgtcgtc tttaaagata
      481 cgtgcagctg ggttcgagat aacgtttgga tgcagcgggt tagttcccag cggcgtttca
      541 aacggctcgt agtgttccgg gaacggacct tccgccatct tatcgagggc aaacagacgc
      601 cccatgcctt cctgctgcat gataaacggc ccgacgccgc tacccggagg cgctgcgctg
      661 tagtccggaa tatcccagcc ggtccactta gtgccgtccc atttcagcaa ctgacgcttc
      721 ggatcccacg ggttaccctg cggatctgcg gaggcgcggt tatacagaat gcggcggtta
      781 agcggccatg cccatgccca gcccagcgtg ttaccgaggc cagacggatc ggcgttatca
      841 cgacgcgcca tctggttgcc ttccggcgtc cagctaccgg cgaaaatcca gcagccacag
      901 gaggttgtac cgtcatcgcg caattgggcg aacgagctaa gttgttggcc tttcttgacg
      961 ataaccgcac cggttgccgg atcggtaata tcggccagcg ccttaccgtt gctctccatc
     1021 gctacttctt ccgacttcgg ctcatgcgga atggcgtagt tccaggtcat gttcagcacc
     1081 tgatccgggt tcgcgccacc ctgttcggca tacatcttgc gcaagcgcag gaagataccg
     1141 gagaggatct caccgtcagt cagcgcaatc cccggggcgt ccgcaccttt ccagtgccac
     1201 tgcaaccagc gacctgagtt aacgattgaa ccgttctctt ctgcgaagca ggtcgatggc
     1261 agacggaaca cttcggtctg gatcttcgac gagtcaactt cgttcagctc accgtggttc
     1321 tgccagaagt tagaggtttc agtgttcagt gggtcgatgg tgacgaggaa cttcagtttc
     1381 gacagacaac cgatcacttt gtttttgttc gggaatgagg caacagggtt aaagccctgg
     1441 cagatatagc cattgacctt gccctctttc atcatctcga agtattgcag gacgtcgtag
     1501 cctttatccc acttcggcaa ccagtcaaag ccccagctat tttccgccgt cgctttatca
     1561 ccaaagaagg ccttcatcat agagacgaag aatttcgggt agttgcccca gtagtttacc
     1621 tggccttcca gcagcggttt tggcgtgttg gcggtaaggt aggtttgcag atcggtctgc
     1681 ttctcgcttg gcagcgtcat gtaacctggc agactctgcg acagtagccc caggtccgtc
     1741 agcccctgaa tattggagtg accgcgcagg gcgttaacgc cgccgccggc catccccata
     1801 ttgccgagca gcagctggat catcgccatc gtacgaatgt tttgcgcacc aacggagtgt
     1861 tgcgtccagc cgagggcata caggaacgag gcagttttat cgtgagcact ggtttctgcg
     1921 atgtattcgc agactttcag gaacgcgtct tttggcgtac cacagatgtt ttcaaccaca
     1981 tctggcgtgt agcgggaaac gtgctgtttc agcaagttcc acacgcagcg cggatgttgc
     2041 agcgtggtat cgcgtttggc gaagccgttt tcgtccagtt cataagtcca ggaggattta
     2101 tcgtacttgc gtttttccgc gtcgtagccg gtgaacaggc catcttcaaa gccgtaatcc
     2161 tcacgcacga tcaggctggc gttggtatag gcttcggtgt attcgtggtt gaatttttca
     2221 ttgttcagca ggtacagcaa tacgcctgac aggaaagcaa tgtcagtacc ggaacgaata
     2281 ggggcatagt agtcagctac cgccgccgta cgcgtaaagc gaggatcgat cacaatcagc
     2341 ttcgcgccgt tgtgaatttt ggcttccatc gcccagcgga acccgaccgg gtgagcttca
     2401 gcggcgttac cgcccatcac cacgacgagg ttggcgttct tgatgtcgac ccagtggttg
     2461 gtcatcgcac cgcgaccaaa tgttggagca agacttgcta ccgttggtcc gtgtcagaca
     2521 cgcgcctggt tgtcgaccgc gagcataccc agcgcgcggg agaatttttg cgttaaatag
     2581 ccggtttcgt tactcgacgc ggaagcacac agcatcccgg tggagagcca gcggttaaca
     2641 gtcacgcctt cggcgttttg cgcaatgtag ttagcatcgc ggtcttcttt catcagtttg
     2701 gcgatgcgat caaacgcctc ttcccaactg atttgctgcc atttatcaga acctggcgca
     2761 cggtattccg gaaacttcag acggctttcg gagtggatga aatccaccag gccagcgcct
     2821 ttcggacaaa gtgcaccgcg gttgaccggg tgatccggat cgccttcgat atggaagata
     2881 gatgctttgg cgttttttgc tccgtcaccg aggctgtaca tcaacagccc acagcctacg
     2941 gaacaatagg tgcaggtatt acgggtttcg cgggtgcgca gcagtttata ctgccgggtt
     3001 tccgcgagtg ctacgctggg tgcaaaaccc agtgccgctg ccgtggtgcc tgccataccg
     3061 ccagcgcaga tcttaaagaa ctgccttctg ctgacctgca tggattgctc cttgtttcga
     3121 cattgtcacg tcccatttac attcgcttgc tgcgtgtgca gggagtggga gttatttttc
     3181 tttgcggaag gggccgcaaa ggtccagaat tggctcaatt tccctccatc taggaagggt
     3241 ttgtaacaga ataccataat gttggtgtgt atgttcttat ctggttaaga gaaagtgaaa
     3301 aaaacacagc gaaaagaaat cgaaaatgtg acaaatatca caggtgttcg tcaaattgag
     3361 ttatggcggc gtgatgattt acaacaccca cggttagatg aggtcgcgga agaagttccc
     3421 gttgcgctgg tctacaacgg catttcgcat gtggtgatga tggcgtcgcc caaagacctt
     3481 gagtactttg cgctcggttt ttcgctttcc gaggggataa tagaaagtcc gcgcgatatc
     3541 ttcggcatgg atgtcgttcc ttcctgtaat ggtcttgaag tacaaattga gctttccagc
     3601 cgccgcttta tggggttgaa ggagcgccgc cgggcgctgg cgggacgtac gggctgtggc
     3661 gtatgcggcg tggagcaact taatgacatc ggaaaaccgg tgcagccgct accgttcacc
     3721 cagacgtttg atctcaacaa actggatgat gcattacgtc atctcaacga ttttcagcca
     3781 gtggggcgac tgactggttg tactcacgtc gctgcctgga tgttgccatc tggcgaactg
     3841 gtcggcgggc atgaagacgt gggtcgccat gtggcgctgg acaaactgtt aggccgccgg
     3901 tcacaagaag gggaaagctg gcagcaaggt gcggtactgg tttccagtcg tgccagttat
     3961 gaaatggtgc aaaagtcggc gatgtgcggc gtagagattt tgtttgcggt gtctgccgcg
     4021 accacgcttg ctgtagaagt ggctgagcgc tgtaatctga cactggtagg tttttgtaaa
     4081 ccgggtcggg caacggttta tacccatccg cagcgtttga gcaattaatt aaaataagtc
     4141 ctgcgatata tattaaattg caggacttat tcatttc
//
LOCUS       DAZOYF010000249         4168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZOYF010000249 DAZOYF010000000
VERSION     DAZOYF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.255"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHUOF_004044"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHUOF_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR0612988.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHUOF_004045"
                     /pseudo
     CDS             complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHUOF_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHUOF_004046"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHUOF_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            3787..>4168
                     /locus_tag="ACHUOF_004047"
     CDS             3787..>4168
                     /locus_tag="ACHUOF_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFR0612989.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1038 a         1208 c         1021 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAZOYF010000250         4064 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZOYF010000250 DAZOYF010000000
VERSION     DAZOYF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.256"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(139..3654)
                     /locus_tag="ACHUOF_004048"
     CDS             complement(139..3654)
                     /locus_tag="ACHUOF_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR0612990.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
BASE COUNT         1055 a         1027 c          844 g         1138 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaa
//
LOCUS       DAZOYF010000251         4064 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZOYF010000251 DAZOYF010000000
VERSION     DAZOYF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.257"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..662)
                     /locus_tag="ACHUOF_004049"
     CDS             complement(<1..662)
                     /locus_tag="ACHUOF_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HFR0612991.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QY"
     gene            complement(720..1244)
                     /gene="sat2"
                     /locus_tag="ACHUOF_004050"
     CDS             complement(720..1244)
                     /gene="sat2"
                     /locus_tag="ACHUOF_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0612992.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(1339..1812)
                     /gene="dfrA1"
                     /locus_tag="ACHUOF_004051"
     CDS             complement(1339..1812)
                     /gene="dfrA1"
                     /locus_tag="ACHUOF_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0612993.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2144..3121
                     /gene="intI2"
                     /locus_tag="ACHUOF_004052"
                     /pseudo
     CDS             2144..3121
                     /gene="intI2"
                     /locus_tag="ACHUOF_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            complement(3309..3548)
                     /locus_tag="ACHUOF_004053"
     CDS             complement(3309..3548)
                     /locus_tag="ACHUOF_004053"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR0612994.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1092 a          941 c          886 g         1145 t
ORIGIN      
        1 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
       61 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg
      121 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
      181 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta
      241 tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag
      301 atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga
      361 taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc
      421 tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt
      481 gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg
      541 ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc
      601 tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc
      661 atgatgttta actcctgaat taagccgcgc cgcgaagcgg tgtcggcttg aatgaattgt
      721 taggcgtcat cctgtgctcc cgagaaccag taccagtaca tcgctgtttc gttcgagact
      781 tgaggtctag ttttatacgt gaacaggtca atgccgccga gagtaaagcc acattttgcg
      841 tacaaattgc aggcaggtac attgttcgtt tgtgtctcta atcgtatgcc aaggagctgt
      901 ctgcttagtg cccacttttt cgcaaattcg atgagactgt gcgcgactcc tttgcctcgg
      961 tgcgtgtgcg acacaacaat gtgttcgata gaggctagat cgttccatgt tgagttgagt
     1021 tcaatcttcc cgacaagctc ttggtcgatg aatgcgccat agcaagcaga gtcttcatca
     1081 gagtcatcat ccgagatgta atccttccgg taggggctca cacttctggt agatagttca
     1141 aagccttggt cggataggtg cacatcgaac acttcacgaa caatgaaatg gttctcagca
     1201 tccaatgttt ccgccacctg ctcagggatc accgaaatct tcatatgacg cctaacgcct
     1261 ggcacagcgg atcgcaaacc tggcgcggct tttggtacaa aaggcgtgac aggtttgcga
     1321 atccgttgct gccacttgtt aacccttttg ccagatttgg taactataat ttatgttaga
     1381 ggcgaagtct tgggtaaaaa ctggcctaaa attgctgggg atttcaggaa agtaaacatc
     1441 accttccggc tcgatgtcta ttgtagatat atgtagtgta tctacttgat cgatcaggct
     1501 tttgtatatc tccccaccac ctgaaacaat gacatgatcc gttattttct ttaggttggt
     1561 taaagcatct ttaattgatg gaaagatcaa tacgttctca ttgtcagatg taaaacttga
     1621 acgtgttacg accgcatact ttcggttggg taatgctccc attgattcaa aagtcttgcg
     1681 tccaaccaac agccattggt tataggtaat agctttaaac aggagctgtt cacctttggc
     1741 actccatgga atatcagggc cattcccgat aactccattc ttcgatatag ctaccattag
     1801 tgatagtttc acaattcttc ctcagaggtt aacattttat tagtaagcat gctcgttttg
     1861 tcacccgctg atgcttaccg ttaattaata taatcacttg tattaaatag actttcttta
     1921 aaaatacaag acactctgtt attacaaatc gtgcatgccg tctatcctgc ttgcacgatg
     1981 cacattatct cacccaaaga actcataaca ttttaattta tttagcatta aattaaaact
     2041 ctaccggtaa acaagcatct ctaggcgtaa aaaacaggca cttttttaaa tcccagctag
     2101 aataggctgt ataggcagac agttgcaaga caaggagcgt tttatgtcta acagtccatt
     2161 tttaaattct atacgcacgg atatgcgaca aaaaggttat gcgctgaaaa ctgaaaaaac
     2221 ttacctgcac tggattaagc gttttattct gtttcacaaa aaacgtcatc ctcagaccat
     2281 gggcagtgaa gaggtcaggc tgtttttatc cagcttagca aacagcagac atgtagccat
     2341 aaacacgcag aaaatcgctt taaatgccct agcttttttg tacaacaggt ttttacaaca
     2401 gccgttgggc gatattgatt atatccctgc aagcaagcct agacggctac cctctgttat
     2461 ctctgcaaat gaagtgcaac gcattttgca ggttatggat actcgcaacc aagttatttt
     2521 tacgctgctg tatggtgcag gtttgcgcat taatgaatgc ttgcgtttgc gggttaaaga
     2581 ttttgatttt gataatggct gcatcactgt gcatgacggt aagggtggga aaagcagaaa
     2641 cagcctactg cccacgcgcc taatcccagc aataaaataa ctcattgagc aagcgcggct
     2701 tattcagcaa gacgacaact tacaaggcgt agggccatcg ctgccttttg ctttagatca
     2761 caaataccct tctgcttatc gacaagcggc gtggatgttt gtctttccct ccagcacgct
     2821 ctgcaaccac ccgtataacg gcaaattatg ccgccatcat ctgcatgact ccgttgcgcg
     2881 aaaggcattg aaggcagccg tacaaaaagc aggcatcgtt agcaagcgtg tcacttgtca
     2941 tacatttcgt cactcgtttg ctacgcatct attacaagcg gggcgtgata ttcgcactgt
     3001 gcaagaactc ttagggcata acgatgttaa gaccacgcaa atctatacgc atgtgttggg
     3061 tcagcatttt gccggcacca ccagtcctgc ggatggactg atgctactta tcaatcagta
     3121 aaagtagata aatatcttta tgtagccctg agtagcccct gatacttttt atggtgaaaa
     3181 agataaacaa tcatagaggg gataaatatg agccagttct accattgttc agataagttt
     3241 aagcaagata ctgtcaatca cagtgaccat tcatggtcat tacacttcct ccagttgcca
     3301 gacaatgcct aacataaaaa cgcaaaagcc caaaagtcag agcttaggtg ttctaggcgt
     3361 tttctaagcg ctgaagaaat acgtcttacc gtagattgcg tgcgtttctg aagcacattt
     3421 tccttataca tggctttatc ccaggcagct tcatcaagct cttggagtaa caagccagta
     3481 ataatgcgac actctcgggt ttgtagcgag ccgctggtga gatcggagtt atagcggaaa
     3541 ggggtcattt gtaattattt gtgggtggag gatagttaac ttattattgt agttggaaga
     3601 ggctgctttc caaacattag cacctcgtaa tgcttgttac tgtatattaa tttatgctct
     3661 tagcgcatgt taatgggcta ttacaatgca taatgtccac atcaagataa ctgtggctta
     3721 acacctgtac aagcgaaaaa aatcaatcac ctgtccggaa atacgggatc actgtaaagt
     3781 gttggtgagg cagtctagga gggggattta atttgggata ctgatgaaag gattatgatc
     3841 agttggatat aggatcgtac taagctgagc ggcaggttag ctagattagt taaccagata
     3901 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
     3961 acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc
     4021 cacccctccc agttcccaac tattttgtcc gcccacagct gccg
//
LOCUS       DAZOYF010000252         4042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZOYF010000252 DAZOYF010000000
VERSION     DAZOYF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.258"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(126..284)
                     /locus_tag="ACHUOF_004054"
     CDS             complement(126..284)
                     /locus_tag="ACHUOF_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0612995.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEI
                     LRVLEVVA"
     gene            complement(886..1077)
                     /locus_tag="ACHUOF_004055"
     CDS             complement(886..1077)
                     /locus_tag="ACHUOF_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR0612996.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="ACHUOF_004056"
     CDS             1142..1465
                     /locus_tag="ACHUOF_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR0612997.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1674..2105
                     /locus_tag="ACHUOF_004057"
                     /pseudo
     CDS             1674..2105
                     /locus_tag="ACHUOF_004057"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="ACHUOF_004058"
     CDS             2143..2673
                     /locus_tag="ACHUOF_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR0612998.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="ACHUOF_004059"
     CDS             2680..2913
                     /locus_tag="ACHUOF_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR0612999.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="ACHUOF_004060"
     CDS             complement(3459..3851)
                     /locus_tag="ACHUOF_004060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613000.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAZOYF010000253         4005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZOYF010000253 DAZOYF010000000
VERSION     DAZOYF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.259"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            98..799
                     /locus_tag="ACHUOF_004061"
     CDS             98..799
                     /locus_tag="ACHUOF_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0613001.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPD
                     EQKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRA
                     QYFKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTG
                     DRTIAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(1057..3204)
                     /gene="mobQ"
                     /locus_tag="ACHUOF_004062"
     CDS             complement(1057..3204)
                     /gene="mobQ"
                     /locus_tag="ACHUOF_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFR0613002.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEAL
                     PKDSNSAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGL
                     AALRAQLLKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGN
                     GEMTCYLLLHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQR
                     VYSSATFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQG
                     RLPPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVI
                     SALTDKGLRYAGYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLF
                     RSLGFNRTWEEWQREEDARTHTTERRHDRGWSQ"
     gene            3383..3637
                     /gene="mobC"
                     /locus_tag="ACHUOF_004063"
     CDS             3383..3637
                     /gene="mobC"
                     /locus_tag="ACHUOF_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="HFR0613003.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
BASE COUNT          951 a         1027 c         1032 g          995 t
ORIGIN      
        1 aatccacaga aaaagggggt tgcaaaaaaa aaacttccac ccatatgata agtggcgtac
       61 agtaaaaaaa cgccaccaat agcaacgagc aagcgaaatg tcagatctga tagcttacaa
      121 atcaaacgcc cttatcgagg caagctacaa gctcactttg caggaacagc gcttcctgct
      181 ggtctgtatc agccgcctaa aatccggaga agattcccct tcgccggatg aacaaaaaac
      241 catgaccatc acggctgcag agtttcatga tgcctttcct gacatgggac ggcagcacgc
      301 tgaagctagg ctcaaggaag cgatagatcg cctctgggac aggtcagtaa tcatcaagaa
      361 cgaggaaaaa cgagaagagt tccgctgggt gcagtaccgg gcgcagtatt tcaaaggtga
      421 ggcgaaagta gatatcacct tctccgatgc catcatgccg tatctgacac agctcaaggg
      481 gcagtttacg cgggtagtcg tgaagaacgt ctccagcctt tccagcacct acagcatccg
      541 catttacgag ctgctccagc agttccgcag caccggtgac agaacgatag ctatcgatga
      601 tttccgaacc atgcttgccg ttgaagataa atatcaacaa ttcaaagact tgaataagat
      661 gataatcaag ccagccatcg ccgaactgaa cgaaaaaagc gatctggttg taactgttga
      721 gacgatcaaa aaggggcgca cggttgtagc cctgcatttt cgcttcaagg aagacaaaca
      781 gatcaagatg acgatctaaa atcgatttta aggcacctaa aacgctccct gctgcgtttt
      841 ttttgtgtta gtagtgcgaa ggtactattg aagtgaaaaa ttgcgtcaaa aacgctctga
      901 tgcagcgaaa aaagaccccc caatttttgg gggcggaaaa acaaaactat gcttgcgtta
      961 tgtgagtgtg cttcttgcat tcatccctgt cgatgaacat cgaagaacat cgacgaacat
     1021 aacacgatgt taaacaaaac aaaatattgt tcacattcac tgtgaccaac cgcgatcatg
     1081 tctcctctcg gtggtgtgtg ttcgtgcatc ttcctcgcgc tgccattcct cccaggtacg
     1141 attaaaaccc agcgagcgga acagatgatc cggctgtttt tctggtgtgt tgacggcagc
     1201 aaaaagcggt gcctcacggg ttttcagatg aaactgcatc agttctggtg caggtgatat
     1261 gtggctgtct gcatcgtgat ttacggcaac accataccct gcataccgta accccttatc
     1321 ggtcagcgca ctgatgacca gatgcctgtt atcggctgag ggaacggcaa acgcgggata
     1381 cagcgtacca ttaagccgga taacaccgcc cagagagtca tccacgccca tgaccagcaa
     1441 aagctgaagc tggcgggttt cccggtcgac agaactcatg accggcatca gcgtcggtgg
     1501 cagacgtccc tgcataaaat ccaccttcct tgccgctggt gatggcatgg ctggctggcg
     1561 gtcttctcca agacagaaag actccggcca tgtcttcggc tggcggagat tgcgggtatg
     1621 ttgcggccat gtctggcaaa cctgcgtcag cagttgctca aaggttgcag aggaataaac
     1681 acgttgcagt ggcggacgtt ggccgatttc gcttcggtca cagatataac cactcctgtc
     1741 acgcattaaa tcaacactct caaagattct ggtgcggtat ttctccagct cgttccccca
     1801 gagcgtatat tctccgttcc ggttgtgaag cagaaggtaa caggtcattt ccccgttacc
     1861 aggcatggga gcctcgccat accccgtaac gactgctctc agcacccctg aatccataac
     1921 ggcattgtca tttttcctga gctgctccgc gcgactacgt tcagtatctt cctgttcctt
     1981 ccggcgcaat tcctcttcac gtttaagcaa ctgggctctg agagctgcaa ggccggattc
     2041 cccccactgc tgccactgtt tttcaaatgc catctgactc gcgggaatgt cctgtttctc
     2101 caccttccgg taatgctgtc gtaatttttc catttgctgg ctcagagact ggatttttcc
     2161 ctgctcacta cggtattccg gtgccgccca ttgcatcagg ggaatacggg aaaaaccgga
     2221 cgaaaacggc gatgattttt tcaggctggc agcatggctc atcgcttcct ttcgttcggt
     2281 gttcagctct tcccagcgtc tgcgcgtcgc ctgcatatct gccatcatcg acatggcatt
     2341 actggtgagt ttccatgccg agttactgtc cttcggcaac gcttcgataa tgtcacgtag
     2401 tgtgtacgaa gttgcgttgt caggaagccc gatattcaga aggtcagcca cacggcgggc
     2461 aggctctgtc agccctccgt tcctgagttc ggtgcgggaa atctgtattt ccaggttaag
     2521 cctgttaatc agacgattct gttcgccgcg ttctgtttcg ataccgcgtg tttccatcgc
     2581 acaggcagac tttccgaggt gaataccagg cgttttctca agcccctgtc gctcgtatga
     2641 acgatggtct atctcttcct gataaccggc gttcgccagt gccctgtttg catggtcagc
     2701 ccatgatgcc cgccacgtct cggcatgtgt ccggtcgttc cagtccctga cctttccgct
     2761 gaacccagca ggtccgatag ccctcgtggt cagcatgatg tgcgcatggg gatttgtttt
     2821 atccatgtga tggaacgcca catcagcaat catgccctga ctgacaaagt tttctctgac
     2881 aaaggcgaga acggtttccc gtgcggcatc ctgcggcagt tcgtggggga tggcgagttc
     2941 aatttcccgc gcaagttgtg agttcttccg gcgttcggcc ttctcaacgg cattccagag
     3001 ttcggcacgg ttcgtacacc aggcaggggc ggtggcgggg gtcagaatca cagcctcagc
     3061 cacaccgctt ttgcgggtgt agtcgtgagt cagtccggta cgctcatcaa gcatcagtga
     3121 accggaacga taggcagcgg aagctacggc gctgtagccg ctgcttctgg agataatttt
     3181 catgctgaga tgataaatcg ccatttctgc cgtaatgcct ttttgtagtt ttctttcagc
     3241 cccgcagggc gcacacaatc gaaacggagt ggagattgta taagtgcgca cttcgtgatt
     3301 tgccgtgcat tatgccagct cagtttttaa actaccagtg acgcgctaaa atgcgatttt
     3361 tcatcgcgca cggagttaca tcatgagcag aacgctcgaa cagaagattg ctgatgctga
     3421 ggcgagatta cagcgcctga aggcgaagag ccggagcctg gacacagcgc agaaggttgt
     3481 tgtgggtgct gcattgctgg caaaggtcag aaagccggag gaggtgcagt tgcgtgcctg
     3541 gttactccag ttcctgaagg cagaggtgac acgacaggct gatgtgacgc gcatactgcc
     3601 gctgattaac gagctggagg cgctgccaga acagtgattg tatcgtccgc tcgccgcagc
     3661 cccagcgacc gagcgttagc gagcgagtgg gcgaggaagc ggaagtgaat cagtcgccag
     3721 gttgagcaca tacgcacggt ttttcggcag gtgatatgcg ctgaacccgc atgaatactg
     3781 ggtttccaca gggttggagg gggtgtattc gaaacttgca attctgcttt attgcaacgc
     3841 attgttttta ttggtttttt gttttcagca ccccaagtta tccacaggct cgccgttaag
     3901 tttttaagat cttattttta gatctttttt tttaaaacct ttttaggtct tgtaacacca
     3961 tgattttaaa gatgttctgg caagataagt ggagcttttg ggggt
//
LOCUS       DAZOYF010000254         3969 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZOYF010000254 DAZOYF010000000
VERSION     DAZOYF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.260"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            160..854
                     /locus_tag="ACHUOF_004064"
     CDS             join(160..406,406..854)
                     /locus_tag="ACHUOF_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0613004.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(973..1371)
                     /locus_tag="ACHUOF_004065"
     CDS             complement(973..1371)
                     /locus_tag="ACHUOF_004065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613005.1"
                     /translation="MNSTINVFGEPSKNLAMLIEEGNLNAPAILNDIKKHVNHLISLH
                     PVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAISIKNHLGQLNLLNSSNIGTLQ
                     INTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(1368..1781)
                     /locus_tag="ACHUOF_004066"
     CDS             complement(1368..1781)
                     /locus_tag="ACHUOF_004066"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HFR0613006.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKN"
     gene            complement(1815..3275)
                     /gene="gadC"
                     /locus_tag="ACHUOF_004067"
     CDS             complement(1815..3275)
                     /gene="gadC"
                     /locus_tag="ACHUOF_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HFR0613007.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            complement(3382..3967)
                     /locus_tag="ACHUOF_004068"
                     /pseudo
     CDS             complement(3382..3967)
                     /locus_tag="ACHUOF_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006663458.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
BASE COUNT         1259 a          818 c          765 g         1127 t
ORIGIN      
        1 taacccgaaa aaatcattgt taatcttatg catttattat ttgattgtaa gttgtgatta
       61 ttataattta gttattgtta gtatgtttta taatgctgga tgggtgatgc ctctaattag
      121 ttgaatctga tgtataatac gggcttttga ggttatctca tgaccagcgt taatattcat
      181 tgtccccgtt gtcagtcagc tcaggtttac cgccatggtc agaaccctaa aggccgtgac
      241 agacttcgct gccgtgactg ccaccgtgtg tttcagttca cttatactta tcaagcacgt
      301 aagccgggta tgaaagagct gataactgaa atggccttta atggtgccgg ggttcgcgat
      361 accgccagga cactgaaaat tggtagtaac accgtcatcc ggactttaaa aaactcgcgc
      421 caaagcgaat aacttcttcg cctgttgccc atgctgatgt ggcgtttatc tgcgagcttg
      481 atgagcaatg gagctacgtt ggcagtaaag cccggcaaca ttggctcggg tacgcgtaca
      541 acaccaaaac aggcggtgta ctggcctaca cttttggtcc ccgaaccgat caaacgtgcc
      601 gggagctact ggcactgctt acacccttca acatcggcat gctgaccagc gatgactggg
      661 gcagctatgg ccgggaggtg ccgaagaata agcatctgac cggcaaaata ttcacccaac
      721 gcattgagcg taataatctg acgctacgca cccgcattaa gcggttgggt cgtaaaacaa
      781 tctgcttctc acgctcagtt gagatccacg aaaaagtgat cggagcgttt atcgaaaaac
      841 acatgttcta ctaattggat gcactaccca gtatgtaact gggtttgtcc ataaaatgca
      901 ccttactcag ttggtgtcca acttttgggg tgcagttcag aaggacggga ttttacggtg
      961 taccggataa atttaaaact gcctgatact gaatgtatga ggtttcgtaa tgctcaattc
     1021 ccctagtacc gttttgtaga tctccatact tccactagta tttatttgca aagttcctat
     1081 atttgaggag tttaataaat ttagctgtcc caagtgattt ttaatagaga tagcctgatc
     1141 atgtgcagga ttaataaatt ttatatccgg tgcaactgct tcaaaaaaat tctgaacaat
     1201 tggatagtga gtacagccga gaataatttc atttactgga tggagggaaa ttaaatggtt
     1261 aacatgtttt ttgatgtcat tcaagattgc aggagcatta agattacctt cctcaattaa
     1321 cattgcaaga tttttgcttg gttctccaaa tacatttatt gttgaattca attttttatt
     1381 agctctttgt gacaccccgt tttaatagta aactcggtag cgataatacc aacattttta
     1441 tattgctggc gaatcacatc ttctactgca ggttcaatga tggataagat aggaaacgga
     1501 aagctcttag catatccgct gtattcttcg gattccaaag ttgaggatat agtattacat
     1561 gcgacagcaa ctagtttgac attccttgac tgtaaaaaag tcagcatttt tttagtcaaa
     1621 ctgatgatat catcggcaga acggttgcca taaggtgcat tcatattatc tccacaatag
     1681 ataatatctt caccaggaag caaattaatt atttccttta ccgatgtcaa tccaccgaca
     1741 cctgagtcta ttacaccaat tggagattca gcactaaaca tatttaacct catcttttgt
     1801 agcttatgga aaatttaatt actagttctt ttttcccaag aaaatggttc gaaatcagag
     1861 tttggatctt tccagtcaga ttttcggaag gcgtaaatta atattggaat taataaaaat
     1921 agggcaacta atactaacaa ataaagaaca tagctggttg gactaccagt tgggatttgc
     1981 tcgggcggaa taaaacttaa tacaaaagcc agaaaagacc ctaagaatcc aagtgttgcc
     2041 cagaaaaata aagcaggggc tcgatatggg cgttttgtat caggttgaga atgtcgtaga
     2101 tagatagcac tagcgaacat gagcatatac ataactaaat agagcataac tgttaactgt
     2161 gataaaatct gaaaagctgc ttgaactgaa ggcattatca caaaagtaac agaaagaact
     2221 gagacaatta taccttgtac aaacattaga tggtgcccca ttccatgacg attggttttt
     2281 tggaaaaact taggtaaata accagctttc gcgatcgcta atacacctgt attaggccct
     2341 gcaatccaag taaccacccc acccagtacg ccaattgcta gcataaatgc catcacagag
     2401 ccaagccatg gcactccagc ccattcgaac agcatatcgt aggcaaccaa caaactttga
     2461 gttagactaa ttttatctgt aggaataata aaggcgaccc ctaaagtact gagcaggaat
     2521 attgcaatag tacctataga agcgatggta attgcaattg ggtatttttt agttggatta
     2581 tctaaatcgt taacatggac cgcattcatt tccatccctg cataagcaag aaatatgctg
     2641 gcggcaagaa caatattctg gaagttagct atattaggga aaagatcacc ccaacctaat
     2701 gggatcaccg ctggtttacc gctgaaccac agataggaga aaccaagaat aacaaggata
     2761 atagctggaa taattgtccc tataataccg caccacttag atacctgcga gaaaatacca
     2821 acgccacgta atgcaacaca aatagctccc cagtaaacaa atagtacgaa aaataaaata
     2881 taccatttat tactggcaat tgattctgcc acccccatat caacatcaat aaatgcaacc
     2941 gagactgcac caaatgtcag tactgttgga aagtagggga tgacttgtag ccaggtcata
     3001 agcattccta caaaacccca gcgagcccca aatgcttctc ctatccatcg aaatgccccc
     3061 cctttttgtg gatatgttgt agctagctca gccgcaacaa aggcaacagg tactaaaaac
     3121 ataattgcag caaaggtata gtaaaaaatg gcacctaagc cgtattctgc ttcagaaggt
     3181 ataaatcgta gacttactac agcagttata ttcatgatag cgagtgtccc cactgtcatt
     3241 ttcttacttt tgggtgcgat attagcatta gacattttta tactctcgaa catttttaaa
     3301 atagaaacat ataattacga tgtaaatata aaaactaata ttaactttaa cacttatctt
     3361 aaaaacacta acgcaatctg ctttacaaaa ataagtaata cctaaagtcg taacgacacc
     3421 aatagtgttt tcatcgacat gcttcaaaac ctctttagca ttcattatca ggtgatgatc
     3481 atcaagtgga atttcacaca atttcacatc acagtaacgg cagaatttat gccagcatat
     3541 ttgcacagga tcgcaaatca ggttcggttg atttaccttg tacttcgcgt tttttacacc
     3601 actgccattt taacgccaag cggccgagca tagcagcttc agatgaacca atggttgagc
     3661 aacctaaggt gtgtcgcgaa tcggggaaac tccacgaatc ggaaagcata cgcacacagc
     3721 gaccttcagg ttccgccatt tgtggatatt catctttata aatcatgttt ttatcaatag
     3781 ctaaattaag caactcacgg atttcatcat caagccatgt ttgacagaaa gtagctaagt
     3841 tttgtcgtga atccccatca aggatgagct catcacgtat ggcgctgaat acatttcgag
     3901 atttcttctc atctacacgc aaatgatatt tatgcaatat aaatgaaagc tcaagtgagc
     3961 tatgaatat
//
LOCUS       DAZOYF010000255         3899 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZOYF010000255 DAZOYF010000000
VERSION     DAZOYF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.261"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..746
                     /locus_tag="ACHUOF_004069"
     CDS             <1..746
                     /locus_tag="ACHUOF_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0613008.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="ACHUOF_004070"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="ACHUOF_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR0613009.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3810
                     /gene="safA"
                     /locus_tag="ACHUOF_004071"
     CDS             3607..3810
                     /gene="safA"
                     /locus_tag="ACHUOF_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR0613010.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
BASE COUNT         1082 a          859 c          918 g         1040 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctggaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgtta
//
LOCUS       DAZOYF010000256         3884 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZOYF010000256 DAZOYF010000000
VERSION     DAZOYF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.262"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(132..1481)
                     /gene="lysC"
                     /locus_tag="ACHUOF_004072"
     CDS             complement(132..1481)
                     /gene="lysC"
                     /locus_tag="ACHUOF_004072"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR0613011.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(1573..1769)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2006..3655
                     /gene="pgi"
                     /locus_tag="ACHUOF_004073"
     CDS             2006..3655
                     /gene="pgi"
                     /locus_tag="ACHUOF_004073"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR0613012.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT          984 a         1033 c          976 g          891 t
ORIGIN      
        1 tttttctgct cattattagc tccttctgat gccattctat ttcaggaagg agtgtccgtt
       61 aaactcaggc tacctcatag tgacaagaaa atcaatacgg cccgaaatat agcttccagg
      121 ccatacagta tttactcaaa caaattacta tgcagttttt gcaccacctg ctcggcatct
      181 tcgccgggca ccaggaagca caggttatgg ctggatgcgc cgtaacaaat catgcgaatg
      241 ttgaacggtt ccagtacgcc gaatacctct ttgccaacgc cgcaggcttt tgacaggtca
      301 ttgccaatca acgcgaccag cgccagacct tcttccacct ccacccggca cagtgcggaa
      361 agctccatca gcagagattg cgtcagcaac gtatcgccag tggaggttga accggtggta
      421 tcaagggtta atgccacgct cacttctgac gtggtgatta agtctaccga aatattatgc
      481 cgcgcgagga tgccgaaaac ttccgcgagg aagccgcgag aatgcagcat attcaggctg
      541 tgcaaagtga gcagagtctg attgcgacga agcgccagcg cgcggaacag cggcggattt
      601 tcagttttat tgcacaccag cgtaccacct gcgcgtgggt ctttgctgga gccgacaaag
      661 accgggatat cgctgcgtac tgcgggtagc aacgttgccg gatgcagtac ttttgcacca
      721 aaagttgcca tctctgccgc ttcggcaaac gcgatttcat caatgcgttt tgctgcggaa
      781 actacgcgtg gatcggtggt gtagatgccc gggacgtcgg tccagatatc aacacgagat
      841 gcgtgtaaag cctccgccag caaggctgcc gtataatcgc tgcctccacg gccaagcgtc
      901 gttgtacgac ctttattttc gctaccgata aatccctggg tgatcactaa gccttcattg
      961 aggcgtggga gcagctgcag cgcggccagt tccgccagcg cggctacatc tggctctgca
     1021 cgaccaaatc ggtcgttggt acgcatcact ttacgtacat caaaccactg tgcctgaaca
     1081 tcgcgttcgc gcaggatttc gacaaacagc agggtcgaca tcagctcgcc gtggctgacc
     1141 agctcatctg tcagcgccgg agacgttgcc agcgccgccg cttctgccag aacagtaatg
     1201 ttctccagca gacgttcaat ctcttcacgg ataacgttcg ggtaacgcag acgttccaga
     1261 atggcaaact ggatgttgcg aatagcgtcg agtttttcga atcgctcgcc aggttccagc
     1321 ccttccgcta aagcgaccag cagattagtg ataccagcag aagccgagag gactactaaa
     1381 cgcacgttgg catcagaaag cacaatatca gcgctgcggt tcatggcgtc aaaatcagct
     1441 acgctggtac cgccaaattt ggagacaaca atttcagaca taactacctc gtgtcagggg
     1501 atccattttc agccttggca caagggaaga gcggaagacg ggtgggcgca gagcgatact
     1561 tcgctactat tttcacccag aagtgctcca ccacttgcga aacgcccgac tgcgaacgct
     1621 tctggtgaca acccagggga ttcagcccct gtagccgatg atgaacgtgg ccagccgttc
     1681 aatcacctcg gcgatgcacc ccctcaggtg ttatcacagg actggctcct ccaacaccgt
     1741 tacttgggca acgcgcctct tctggcctgc gctagcgcag gtagtacatt tataaataaa
     1801 gggtgagcgg ggcggttgtc aacgacgggg tcatgcggat ttttcatcca ctcctggcgg
     1861 tcagtagttc agctaataaa tgcttcactg cgctaagggt ttacactcaa cattacacta
     1921 acggcactaa aaccatcaca tttttctgtg actggcgcta caatcttcca aagtcacaat
     1981 tctcaaaatc agaagagtat tgctaatgaa aaacatcaat ccaacgcaga ccgctgcctg
     2041 gcaggcacta cagaaacact tcgatgaaat gaaagacgtt acgatcgccg atctttttgc
     2101 taaagacggc gatcgttttt ctaagttctc cgcaaccttc gacgatcaga tgctggtgga
     2161 ttactccaaa aaccgcatca ctgaagagac gctggcgaaa ttacaggatc tggcgaaaga
     2221 gtgcgatctg gcgggcgcga ttaagtcgat gttctctggc gagaagatca accgcactga
     2281 aaaccgcgcc gtgctgcacg tagcgctgcg taatcgtagc aataccccga ttctggttga
     2341 tggcaaagat gttatgccag aagtcaacgc ggtgctggag aagatgaaaa ccttctcgga
     2401 agcgattatc tccggtgagt ggaagggtta taccggcaaa gcaatcactg acgtagtgaa
     2461 catcgggatc ggcggttctg acctcggccc gtacatggtg accgaagcac tgcgtccgta
     2521 caaaaaccac ctgaacatgc actttgtttc taacgtcgat gggactcaca tctcggaagt
     2581 gctgaaaaaa gtaaacccgg aaaccacgct gttcctggta gcatctaaaa ccttcaccac
     2641 tcaggaaacc atgaccaacg cccatagcgc gcgtgactgg ttcctgaaag cggcaggtga
     2701 tgaaaaacac gttgcgaaac attttgcggc gctctcaacc aatgccaaag ccgttggcga
     2761 gtttggtatt gatactgcca acatgttcga gttctgggac tgggtcggcg gccgttactc
     2821 tttgtggtca gcgattggcc tgtcgattgt tctctccatc ggctttgata acttcgttga
     2881 actgctttct ggcgcacacg caatggacaa gcatttctcc accacgcctg ccgagaaaaa
     2941 cctgcctgtc ctgctggcgc tgattggcat ctggtacaac aatttctttg gtgcggaaac
     3001 tgaagcgatt ctgccgtatg accagtatat gcaccgtttc gcggcgtact tccagcaggg
     3061 caatatggag tccaacggta agtatgttga ccgtaacggt aacgttgtgg attaccagac
     3121 tggcccgatt atctggggtg aaccaggcac taacggtcag cacgcgttct accagctgat
     3181 ccaccaggga accaaaatgg taccgtgcga tttcatcgcc ccggctatca cccataaccc
     3241 gctctctgat caccaccaga aactgctgtc taacttcttc gcccagaccg aagcgctggc
     3301 gtttggtaaa tcccgcgaag tggttgagca ggaatatcgc gatcagggta aagatccggc
     3361 aacgcttgac tacgtggtgc cgttcaaagt gttcgaaggt aaccgcccga ccaactccat
     3421 cctgctgcgc gaaatcactc cgttcagcct gggtgcgttg attgcgctgt atgagcacaa
     3481 aatctttact cagggcgtga tcctgaacat cttcaccttc gaccagtggg gcgtggagct
     3541 gggtaaacaa ctggcaaacc gtattctgcc agagctgaaa gatgataaag aaatcagcag
     3601 ccacgatagc tcgaccaatg gtctgattaa ccgctataaa gcgtggcgcg gttaatcatc
     3661 atgcgtgcct gatgccgata tgtaggccgg ataaggcgtt tacgccgcat ccggcaaccg
     3721 gagcctgatg ccaatatcga cattaatgca tagtatgagg agttaaataa ccttaaaaac
     3781 aatgccgaca ataagtcggc attgttttat cagataaatc cccttgtctg tgatttaacg
     3841 gaaatcatac cgtgaggtta atcctaaaat agatttttaa tcgt
//
LOCUS       DAZOYF010000257         3872 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZOYF010000257 DAZOYF010000000
VERSION     DAZOYF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.263"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            112..1446
                     /gene="adeQ"
                     /locus_tag="ACHUOF_004074"
                     /gene_synonym="yicO"
     CDS             112..1446
                     /gene="adeQ"
                     /locus_tag="ACHUOF_004074"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR0613013.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1499..1951
                     /locus_tag="ACHUOF_004075"
     CDS             1499..1951
                     /locus_tag="ACHUOF_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR0613014.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2162..3352
                     /gene="nepI"
                     /locus_tag="ACHUOF_004076"
     CDS             2162..3352
                     /gene="nepI"
                     /locus_tag="ACHUOF_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFR0613015.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3393..3686)
                     /gene="yicS"
                     /locus_tag="ACHUOF_004077"
     CDS             complement(3393..3686)
                     /gene="yicS"
                     /locus_tag="ACHUOF_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR0613016.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
BASE COUNT          798 a          862 c         1022 g         1190 t
ORIGIN      
        1 attaaagaat atctattaat gtgcaattga aatgtgataa ttatcactga accgttgttg
       61 tcatttgttt tacaaaagca agggattaaa tctcctccaa tggacaaaaa aatgaataat
      121 gacaataccg attacgtgag taatgaatca gggacgcttt cgcgattatt taaactacct
      181 cagcatggga ccaccgtccg cacagaattg attgcgggga tgaccacttt tttaaccatg
      241 gtgtacattg tttttgtgaa cccgcaaatc ctcggcgcgg cacaaatgga cccgaaagtg
      301 gtgtttgtta ccacctgttt gattgccggt atcggcagta ttgcgatggg gatatttgct
      361 aacttacccg tggcgctggc tccggcaatg gggctgaacg ccttctttgc gttcgtggtc
      421 gtgggggcga tggggatctc ctggcagacc gggatgggcg cgatattctg gggcgcaatt
      481 gggctatttt tgctcacgct gtttcgtatc cggtactgga tgatctccaa cattccatta
      541 agtttacgta ttggtatcac cagtggaatc ggattattta ttgccttaat gggattaaaa
      601 aacactggtg ttattgtcgc caataaagac acgctggtga tgattggcga tttaagttct
      661 cacggcgtgt tgttaggtat tttagggttt tttattataa ccgtgttgtc atcacgtcat
      721 tttcatgccg cggtgctggt ttctattgtg gtgacgtctt gctgtggatt atttttcggt
      781 gatgttcatt ttagcggcgt ctattccatt ccgcctgata ttagcggcgt cattggtgaa
      841 gtagatttga gcggcgcgtt aacacttgaa ctcgccggta tcattttctc ctttatgctg
      901 atcaacctat ttgattcatc aggaacatta attggtgtaa ctgataaagc gggcttaata
      961 gatagcaacg gtaaattccc caatatgaat aaggcgctgt atgttgatag cgtcagttcg
     1021 gtggcaggcg catttatcgg tacctcgtct gttactgcct atattgaaag tacttctggt
     1081 gtggcagtcg gtggtcgcac ggggctaacg gctgtcgtgg tcggcgttat gttcctgtta
     1141 gttatgttct tctcaccgct ggtggcgatg gttcctcctt acgcaaccgc tggagcgtta
     1201 atctttgttg gcgtgctgat gacttcgagc ctggcgcgcg ttaactggga tgattttacc
     1261 gaatcggtgc ctgcgtttat taccacggtg atgatgccct ttactttctc gatcaccgaa
     1321 gggattgcac tcggctttat gtcgtactgc atcatgaaag tgtgcaccgg gcgctggcgc
     1381 gatctgaacc tgtgtgtggt ggtggtcgca gctctgttcg cattgaagat tattctggtg
     1441 gattagtaac tggcgagggt ttccagatat catgagttct gattacgcag gagaactcat
     1501 gatctggata atgctcgcca cgctggcggt agtgtttgtg gttggttttc gggtgctgac
     1561 atccggggcc agaaaagcga ttcgccgtct cagcgatcgg ctgaatatcg atgtcgtacc
     1621 cgtggagtcg atggtcgatc aaatgggaaa gtcagccggt gacgaatttt tacgttattt
     1681 gcatcgcccg gatgagtcgc acctgcaaaa cgccgcgcag gtgttgctta tctggcaaat
     1741 tgtcattgtc gatgatagcg aacagaacct gctgcaatgg caccggattt tacaaaaagc
     1801 ccgcctttcc gcgccgatta ccgatgctca ggtcaggctg gcgctgggtt ttctgcgtga
     1861 aaccgaacct gaaatgcagg atattaatgc ttttcagatg cgctataacg cgttctttca
     1921 gcctgccgag ggcgttcact ggttgcattg aaaccgtttc gtcttatcag cctacagacc
     1981 gcgcctgacc tgtcgggtgg ataagacgtt cacgccgcat ccggtaatct ttgccctgtt
     2041 ccgtttcgat cttaactcca gctcaagcaa caccgtaaaa gttgcaaaaa cgttaaattc
     2101 gtcacacatt tcaatgttaa agtggtcggc ttttcccctg aaacatgcca cgggtaacac
     2161 catgagtgaa tttattgccg aaaaccgcgg cgcggatgcc atcacccgac cgaactggtc
     2221 agccgttttc tcggtggcgt tttgtgtcgc ctgtctgatt atcgttgagt ttttgcccgt
     2281 cagtttgttg acgccaatgg cccaggattt aggcatttcg gaaggggttg ccgggcaatc
     2341 ggtgaccgtg accgcctttg tggcaatgtt tgccagtttg tttattaccc agacaattca
     2401 ggccactgac cgccgctacg ttgttatttt gtttgccgtt ttgctgacgc tctcctgctt
     2461 gctggtttcc tttgctaact cattcagttt gcttttaatc ggtcgtgcct gtctggggct
     2521 ggcgctgggc gggttctggg cgatgtcggc gtcgctgacc atgcgtctgg taccgccgcg
     2581 tacggtgccg aaggcgctgt cggtgatctt cggcgcggtt tctattgcgc tggtgattgc
     2641 cgcgccgttg ggcagttttt taggcgagct tatcggttgg cgcaatgtct ttaatgcggc
     2701 ggcggtgatg ggcgtgctgt gtattttctg gattatcaaa tcattgcctt cactgccagg
     2761 cgaaccctcg catcagaaac aaaatacttt ccgcttatta caacgtccgg gtgtgatggc
     2821 agggatgatc gccatcttca tgtctttcgc cgggcagttt gctttcttca cgtatattcg
     2881 cccggtgtat atgaacctgg cgggattcgg cgtggatggc ttaacgctgg tgctgttgag
     2941 ttttggtatc gccagcttta ttggtacgtc gctttcgtcg ttcattctta aacgttcggt
     3001 aaaactggcc ttagcaggcg cgccgttaat actggctgtg agtgcgttgg tactgacgtt
     3061 gtggggaagc gataaaatcg ttgctaccgg cgtggcgatt atctgggggc taacttttgc
     3121 attggttccc gtcggctggt caacgtggat cacccgctcg ctggccgatc aggcagaaaa
     3181 agccgggtct attcaggtgg cggttattca gcttgctaat acctgtggcg cggcaatcgg
     3241 cggttatgcg ctggataata ttggtctgac ttcgccgttg atgttgtccg gcacattgat
     3301 gttgctgact gcattgttgg ttactgcaaa ggtgaaaatg aagaaatcct gatatcaggt
     3361 aaagaagcgt tggtcagtag ttgttgctgt tcttacatat ccgggcattc taccttaccc
     3421 aacgcttccc aataattgct ctgattatta cgttccatcg ccacgatggc ctcgcgtata
     3481 tgttgtttat tgttttcatc tgacaacatt aacttttccc acgcttcatc tgataatgac
     3541 gcatgtggcg tgcaaatttt acgtaaatct tgtaataccg tggttttctc ttttgcggac
     3601 tgcagtgagc taaaaggcga ctcggcgtat acgatgcctg gcatagcgaa aaaagtgaaa
     3661 ataagcagta gtgtcgttgg tttcatggat tcaacctttt acaaatgtgt atcgaatgaa
     3721 gcataagtgt agtttgcttt catcgctctc atcatgctgt acgaaacgcg cttgcgtggt
     3781 cagtaagaag tgccagactt tatattccac ttttattcct ttttattctt atcgatagcg
     3841 tttcgttttt taaaccgcag cgaccttacc gc
//
LOCUS       DAZOYF010000258         3807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZOYF010000258 DAZOYF010000000
VERSION     DAZOYF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.264"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            437..2090
                     /gene="ushA"
                     /locus_tag="ACHUOF_004078"
                     /pseudo
     CDS             437..2090
                     /gene="ushA"
                     /locus_tag="ACHUOF_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
     gene            complement(2101..2271)
                     /locus_tag="ACHUOF_004079"
                     /pseudo
     CDS             complement(2101..2271)
                     /locus_tag="ACHUOF_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2404..2523)
                     /locus_tag="ACHUOF_004080"
                     /pseudo
     CDS             complement(2404..2523)
                     /locus_tag="ACHUOF_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2597..3596)
                     /locus_tag="ACHUOF_004081"
                     /pseudo
     CDS             complement(2597..3596)
                     /locus_tag="ACHUOF_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1053 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tcataattgg ttatgatgca atggccgtcg
      781 gtaatcatga atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat
      841 ttcctttcct ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat
      901 ggaaaatttt catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata
      961 ctgcgaaaac aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg
     1021 aggcgaggag tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg
     1081 caacacatat gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg
     1141 aaatggctcg cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag
     1201 ctcctgtctg catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct
     1261 gtgtaccaga taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg
     1321 tcggtcaggc tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac
     1381 acccagttaa tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt
     1441 acacccccga aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata
     1501 aaggcaaagc acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca
     1561 gaagtaaagt tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag
     1621 aacgtattga cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga
     1681 gtggtaacat cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa
     1741 gtattgattt gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac
     1801 cggattccgg agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa
     1861 tcgaaatgct aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa
     1921 catttagctt taatgctact ggtggggatg gctatccacg tattgataat agacccggat
     1981 acataaatac cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac
     2041 cgctggatgc tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt
     2101 gacgatcatc acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg
     2161 tatgtttgcg atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt
     2221 gctgaattta accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt
     2281 cgctgattaa agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat
     2341 tgatatttac taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc
     2401 cggtcagtcc tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt
     2461 cagacggcat gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga
     2521 accccgccgg agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc
     2581 gtcatacggc tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct
     2641 tcggatactg tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt
     2701 atttagactg catccactta agcaaacgtg actgcattgc actccacagt cgatagctga
     2761 agtttctgga ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc
     2821 gcctcgcaaa atccagcaaa ctctcagctg ttgagcgatg tatacgccac ttcttgattg
     2881 tttctgttat tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt
     2941 cgcctttgaa attcttcaga gtacgcactt tgaagtcata tccgagagtg gcatacaaca
     3001 tcatcggcgt acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag
     3061 gcatagtgca ggaacagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct
     3121 cgaccccgtg ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca
     3181 atgtacatca ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca
     3241 tgatcgatat catcaacgaa gcctttaata tcgacttcca ccacccagtc aaacttccag
     3301 cagcgtttcc tcgcctgcgc aatcgcctgg tgtgcggatt tacccggtcg ataaccatac
     3361 gagtctgcat ggaacagggg ttccagtacc ggctcgagta tctgcttaac gaccatctgg
     3421 gcgatccggt ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga
     3481 atgtccacac gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt
     3541 ttgtagagat tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct
     3601 gccgccccac ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca
     3661 ggaaatggct ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc
     3721 gccgcaccat cagcacaatg gggcaagccc tttgctccgg acccattacg gtccttcacc
     3781 gctactacgg tttactccgt ccctgta
//
LOCUS       DAZOYF010000259         3732 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZOYF010000259 DAZOYF010000000
VERSION     DAZOYF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.265"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            140..586
                     /locus_tag="ACHUOF_004082"
     CDS             140..586
                     /locus_tag="ACHUOF_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR0613017.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            597..2048
                     /locus_tag="ACHUOF_004083"
     CDS             597..2048
                     /locus_tag="ACHUOF_004083"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR0613018.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2038..3108
                     /locus_tag="ACHUOF_004084"
     CDS             2038..3108
                     /locus_tag="ACHUOF_004084"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR0613019.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            3108..>3732
                     /locus_tag="ACHUOF_004085"
     CDS             3108..>3732
                     /locus_tag="ACHUOF_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFR0613020.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT          836 a          975 c         1010 g          911 t
ORIGIN      
        1 taaccattat gtgcattact ccgcaaatct gacctttcac tctgtctcaa attgtcattg
       61 tttaccgaca tatcgcctct catcacttaa tcgctccacc attacaattg attaaaatat
      121 attttatgga gtccgattaa tggcagctct tactgcaagc tgtattgacc tgaatattca
      181 gggcaatggc gcttattccg ttctgaagca gttggcgaca atagcgttac aaaacggttt
      241 tatcaccgac tcacaccagt tcctgcaaac cctgctgctg cgcgaaaaaa tgcactctac
      301 tggatttggt tccggtgtcg cagtgccgca cggtaaaagc gcctgcgtta aacaaccgtt
      361 cgtattattc gcccgcaaag cgcaggctat tgactggaaa gccagcgatg gcgaagacgt
      421 caattgctgg atctgcctcg gcgtgccgca aagcggcgaa gaggatcagg tcaaaatcat
      481 cggcacactg tgtcgcaaaa ttattcacca ggaatttatt catcaactgc aacagggcga
      541 taccgaccag gtgctggcct tgttaaatca aaccctcagc tcataaggaa gtggcgatgg
      601 agtcatcctt acgtattgtc gcgatcacca actgccccgc cgggatcgct cacacctaca
      661 tggtggcgga agccctggaa cagaaagccc gttctctcgg ccataccata aaagtggaaa
      721 ctcaagggtc cagtggcgtt gaaaaccgct tatccagcga agagattgcc gctgccgatt
      781 acgtcattct tgctaccggg cgtggcctga gcggtgatga tcgcgcgcgg tttgccggga
      841 agaaagttta tgagattgcc atctccctgg cgttgaaaaa tatcgaccag attttcagcg
      901 aattaccgac aaactcgcag ctttttgccg cagatagcgg cgtgaagctt ggcaaacagg
      961 aagtgcaaag cggcagcgtg atgagccacc tgatggcggg cgtctctgcg gcgctgccgt
     1021 ttgtcatcgg cggtggtatc ctggtggcgc ttgccaacat gctggtgcag ttcggtttgc
     1081 cgtataccga tatgtcgaaa ggcgcgccgt cgtttacctg ggtagttgaa tccatcggtt
     1141 accttggctt cacctttatg atccccatca tgggcgctta tattgcctcg tcgattgccg
     1201 ataaaccggc tttcgctccg gcgtttctgg tttgctatct ggcgaacgac aaagcgctgc
     1261 tcggcaccca gtcgggtgcg ggattcctcg gcgcggtggt gctggggctg gcgattggct
     1321 atttcgtctt ctggttccgc aaagtgcgcc ttggcaaagc attacaaccg ctgctcggtt
     1381 caatgctgat cccgttcgtt actctgctgg ttttcggcgt gctcacttac tacgttatcg
     1441 ggccggtgat gtccgacctc atgggcgggc tgctccactt cctgaatacc attcctccat
     1501 caatgaagtt tgcggcagcg tttctggtcg gtgcgatgct ggcgttcgat atgggcggcc
     1561 ccatcaataa aaccgcctgg ttcttctgct tctcactgct ggaaaaacac atttatgact
     1621 ggtacgccat cgtcggcgtt gtcgcgttga tgccgcccgt tgcggcgggt cttgcgacgt
     1681 tcattgcgcc caaactgttt acccgacagg aaaaagaggc tgccagtagt gccattgtgg
     1741 tcggtgccac agtcgcaacc gaaccggcta taccttacgc tctggcagct ccgttgccga
     1801 tgattaccgc caatacgctg gcgggcggca ttaccggcgt gctggtgatc gctttcggaa
     1861 tcaaacgtct ggcacctggt ttgggtatct ttgacccgct gatcggcctg atgtcgccgg
     1921 taggttcgtt ttatctggtg ctggcgattg gcctcgcgct gaacatttca ttcattatcg
     1981 tcctgaaagg attgtggtta cgccgcaaag cgaaagccgc gcaacaggag cttgtccatg
     2041 aacattgagt tactgcaaca gttgtgcgaa gccagcgccg ttagcggcga tgaacaggaa
     2101 gttcgcgaca tcctgataaa cacgctggaa ccttgcgtta atgaaatcac ctttgatggt
     2161 ctgggcagct ttgttgcccg taagggaaat aaaggtccaa aagttgccgt tgtcgggcat
     2221 atggatgaag tcggctttat ggtcacccac atcgacgaga gcggttttct gcgtttcacc
     2281 accattggtg gctggtggaa tcagtcgatg ctcaaccatc gggtaaccat acgcacacac
     2341 aagggagtga aaatccctgg tgtgattggt ttcgtcgcgc ctcatgcgtt aacggaaaag
     2401 caaaggcaac agccgctgtc atttgatgag atgttcattg atattggcgc aaacagtcgc
     2461 gatgaggtgg aaaaacgcgg tgtggcgatg ggcgatttta ttagcccgga agccaatttt
     2521 gcctgctggg gcgaagataa agtggtcggc aaggcgctgg ataaccgcat cggctgcgca
     2581 atgatggctg aactattgca gacggtgaat aatcccgaaa ttacgctgta tggcgtcggc
     2641 agcgtggaag aagaagttgg gctacgcggg gcacaaacct cggcggaaca cattaaaccg
     2701 gatgtagtga tcgtgctgga taccgctgtc gcaggcgatg ttccgggcat tgataacatt
     2761 aaatacccgc tgaaactggg ccaggggccg gggctgatgc tgtttgacaa gcgctacttc
     2821 cccaaccaga aactggtagc agcgttaaaa aactgtgccg cacataacga tttaccgctg
     2881 caattttcca ccatgaaaac cggagcgacg gatggcgggc gctacaacgt gatgggcggc
     2941 gggcgtccgg ttgttgcgct gtgcctgcca acccgctatc tgcacgctaa cagcggcatg
     3001 atttcaaaag ccgattacga tgctctgctc acgttgatac ggggttttct gacgacctta
     3061 accgcggaga aagtcaacgc gtttagccag ttccgtcagg tggattaatg ctaaacgaac
     3121 gccagttaaa gattgtcgat ctgctggagc aacagccgcg cacgcctggt gagctggcgc
     3181 aacagactgg cgtttcaggc aggaccatcc tgcgtgatat tgactatctc aactttaccc
     3241 ttaacggcaa agcccgcatt ttcgccagtg gcagtgcggg ctatcagctg gaaatcttcg
     3301 agcgccgcag cttttttcag ttgctgcaaa agcacgataa cgacgatcgg ctgctggcgc
     3361 tgttattact gaatactttc actccccgtg cgcaactcgc ctcggcgctt aatttgccag
     3421 aaacgtgggt agcagagcgt ctgccccagt taaaacagcg ttatgaacgc acttgttgcc
     3481 tggccagccg ccctggtttg ggccatttca ttgatgagac agaagagaaa ctcgttatct
     3541 tgctggcgaa cttgctgcgc aaagatccgt ttttaattcc gctggcgggc ataacacgag
     3601 acaaccttca gcatttatcc acggcctgcg acaaccaaca ccgctggccg ctcatacagg
     3661 gtgattatct ctccagcctg attctggcga tttacgccct gcgtaatcaa ctgaccgatg
     3721 agtggccgca at
//
LOCUS       DAZOYF010000260         3717 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZOYF010000260 DAZOYF010000000
VERSION     DAZOYF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.266"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1745)
                     /gene="rsxC"
                     /locus_tag="ACHUOF_004086"
     CDS             complement(<1..1745)
                     /gene="rsxC"
                     /locus_tag="ACHUOF_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR0613021.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPE"
     gene            complement(1738..2316)
                     /gene="rsxB"
                     /locus_tag="ACHUOF_004087"
     CDS             complement(1738..2316)
                     /gene="rsxB"
                     /locus_tag="ACHUOF_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR0613022.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(2316..2897)
                     /gene="rsxA"
                     /locus_tag="ACHUOF_004088"
     CDS             complement(2316..2897)
                     /gene="rsxA"
                     /locus_tag="ACHUOF_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR0613023.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(2974..3414)
                     /locus_tag="ACHUOF_004089"
     CDS             complement(2974..3414)
                     /locus_tag="ACHUOF_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR0613024.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(3500..3715)
                     /gene="ydgT"
                     /locus_tag="ACHUOF_004090"
     CDS             complement(3500..3715)
                     /gene="ydgT"
                     /locus_tag="ACHUOF_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR0613025.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
BASE COUNT          953 a          991 c          942 g          831 t
ORIGIN      
        1 tcttctggtt ccgcattagc ctgttgctgt tccagcttgc gcgctttgga gcgggcgata
       61 gctgcttcaa cggcagtttt acgtggttca gcaacggttg ctgcgtcgtt agtttgctcc
      121 agttctgcct gtttcgctct ggcttgcgct ttacgggctt cccgttctgc aataattgca
      181 ctgttatccg ggcgttcgcc cgctttaatc acaataggct gtgtagcctg ggcctgtttc
      241 tctttcaccc gcgccagagc ggcagcaatc gcatctttat ctttggctgc aggttgaacg
      301 gctgcgctct tatgtcgttc aaggcgagtc gctttttcgc gctccagacg agcctggcgc
      361 gcttcgaaac gcgctttggc ttctgcggcg cgcttttctt cctgacgaat agccgcaatt
      421 tcagcttttt cctgacggaa atattgcacc aagggaatat tgctcgggca aacccacgcg
      481 caagccccac attcaatgca gtcggcaatg ttatgcgtgg tagctttatc gtgttgctga
      541 cctttgctga accagtacaa ctgttgcggc aaaagatcca cagggcaggc gtcagcacag
      601 gcgctacacc ggatgcagct ttgttcttcc tgtggttcgc caagttcatt ggcagaggga
      661 gccaacagac agttggtaat ctttacgacc gggacatcca gccatggcaa ggtaaagccc
      721 attagcgggc cacccataat caccatttga tcggcagagg ggcagaatcc ggcatcattc
      781 aataaatgac gcactggcgt ccccagccgt gcccagacgt tgcccgggcg agcgattgct
      841 tcgccagtca gggttacaac acgctcggta atcggctcgc catcaataac ggcacgtttc
      901 actgcataag cagtgccgac gttttgcatt aatacgccga tatcggatga acgcccgcca
      961 tgtggaacct gcttcccggt cagaatgtag gttaattgtt tagcaccgcc agaaggatat
     1021 ttggttggaa tcacccgcag agaaatatcg ttagagtccg ccagcaccgc gcgcagcatg
     1081 gaaatcgcct gcggtttgtt atcttcaatg ccgataagaa tttcgcgtgg ctgcagaata
     1141 tgcgcaagaa tgcgaatacc ctctacgacc tgagccgcgc aatcctgcat caaacggtca
     1201 tcggcggtaa tgtacggctc gcactcagca gcgttgataa tcaacgtttc aatcttatct
     1261 ccaccaccct gcaatttaac gcctgtcggg aatccggcac cgcccagccc ggcaacacca
     1321 aactgatgta tgcgctcgat taactcttcg cgactgcgag agcgataatc gacccagccg
     1381 tcgcgcggga tccagcagtc ttcaccatcg gcatcaataa tcacgcttaa ttcagctaaa
     1441 gctgaaggat gagccgtaga gtggggcgca atagccgtaa cggtacccga ggtgggcgcg
     1501 tgaacaggca gcattttgcc gcgaccacgg gtaagcggct ggccgcgcaa tactttatcg
     1561 ccgacgctaa cgcacaactc accttcagcg ccaatatgct gtttcagtgg aataacaaaa
     1621 tgctgcgcca gtagtacctg gcgcaggggg gtaccgttgg tctgggtttt catctccggt
     1681 ggatggatgc cgccgttgaa atcccagatt ttattttttc tgaatgcaga gaataactta
     1741 agcatggtgt tccacgggaa tgatacgcac gggaatggtg ttcagatccc atttccagga
     1801 gtcaggtgtt tctgcgaccg gttgcaacga gatgcagtgc gtcgggcacg gatcaacaca
     1861 taaattgcag cccgtacaga gatcactcat taccgtatgc atgacacggg tagcgccaac
     1921 gatggcgtct accggacaag cctgaataca tttagtgcag ccaatacagt tattttcatc
     1981 aataaccgcc accatccgcg caggcgttag ctcttgcgct tcgccatcca gcggctgcgg
     2041 ctcgacatta agcaactcgg caatttttag catcacagct tcgccacctg gggcgcaacg
     2101 gttgattttt tcaccgttac agctgatggc ttccgcgtag gggcgacagc cgggataacc
     2161 gcactgacca cactggctct gcggtaagat ttcgtcaatt ttctcaacga ccggatcgtc
     2221 ttccaccgca aaacggcggg aggcataacc cagaatggcg ccaaacgcca ggcccagcag
     2281 gctcacggcg gcaacggcaa tccagatagc attcattaca acttcaccaa accactaaag
     2341 cccataaagg ccagagacat aagacctgcg gtaattaacg caatggcatt accgcgaaaa
     2401 ggtgccggga catcagccac agcaaggcgt tcgcggatgg cggcgaagag caccatcacc
     2461 agcgagaaac cgacagcggc ggaaaaaccg tacagcgccg actgcaagaa attgtgcccg
     2521 agattgatat tcagcaacgc cacgccgagc actgcacagt tggtggtgat aagcggcaaa
     2581 aaaatcccca gcagacggta aagcaccggg ctggttttgc gcaccaccat ctcggtgaac
     2641 tgcacgacca cagcaatcac cagaataaat gccagggtgc gcaggtaaat cagattaagt
     2701 gggatcaaaa tccacgtatc gataagccag gcgcaaatag acgtcagcgt catcacaaac
     2761 gttgttgcca gccccatgcc catcgcggtt tccagttttt tggaaacccc cataaacgga
     2821 cagagaccga gaaacttgac cagtacaaag ttattgacca gtacagttcc gacaaacagt
     2881 aacaggtagt cagtcatttt tcggcctgaa ataaaaaagc cgcctattat ccgttaatcc
     2941 agagcaggcg acaacaggtt aactgtaagg ttattacgga ttcacaaacg tcgcttttac
     3001 ccgcgacgag cgtttgaaat aggggacgat caatgcggtt gccaacaaag taaacagcaa
     3061 ctggcgaacg gctatgccaa cttccacggg tgaaaaagcg aatgctttta ctgccagcaa
     3121 tacggaaata agcagccaga taatatagtg tttaggaaca caacgccgac gtttaaagaa
     3181 agcgatggtc aaccacagtg tgtaatacca catggcgata gcggtaataa aagaaacgcc
     3241 ccataagatt tgtgtcgtca gtgcttgtcc gccgagcgtt tgaaatgttt gcggagaaga
     3301 taacgcagcg gtatacagca acaacgccag cgtcgtactc aacagagcga ctaaaagcca
     3361 ggccaacggg ccaagcaacc agcctccaat acgttgtggc gttgtggtgg tcataggttc
     3421 tccagaatag cgacgcaaac aggcaaatct aattttaggg gcgttattat aaggctttta
     3481 taaccgatcg ctactttctt tattggacat agtgccagac ggacttcggt acctggccga
     3541 ggtcaaacaa acgcccgcca gaaaccagct ctgcgcgacg gtgatcggca gcacgataca
     3601 tattgatcag ttcctgatcg tccgtcaggg tgtaattaag atggtcgtag agtttttcca
     3661 gactttcgag tgaactgatt ttgcgaaatt ttaataagta gtcctgaaca gtcataa
//
LOCUS       DAZOYF010000261         3691 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZOYF010000261 DAZOYF010000000
VERSION     DAZOYF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.267"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            108..2105
                     /locus_tag="ACHUOF_004091"
     CDS             108..2105
                     /locus_tag="ACHUOF_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFR0613026.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..3445
                     /locus_tag="ACHUOF_004092"
     CDS             2168..3445
                     /locus_tag="ACHUOF_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HFR0613027.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          891 a          839 c          931 g         1030 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata gattctgata gatatctaat aaaatcaact tattataatt tatttagtgg
     3421 cgaatattct aataaaaaaa catagctgca gagcaagcaa ttcggttgtt ggcaagtcaa
     3481 gggcaaagca ttcgcgtgag aatgggcgta tgctttgtgc caaaagcaaa cgttgctaat
     3541 tctgcactgt tcagcgatgg cgcagtagag acaggcactg ccactgtcca gtcggcaaaa
     3601 gcgaacactt tgccactgcg gggataaact gattgaagct ctcgcgcaaa atcacttatt
     3661 cttttgcgtt aataaaatgt aggagatggg t
//
LOCUS       DAZOYF010000262         3676 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZOYF010000262 DAZOYF010000000
VERSION     DAZOYF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.268"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(47..91)
                     /locus_tag="ACHUOF_004093"
     CDS             complement(47..91)
                     /locus_tag="ACHUOF_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613028.1"
                     /translation="MKRNDKILHYEGLN"
     gene            210..1934
                     /gene="ilvI"
                     /locus_tag="ACHUOF_004094"
     CDS             210..1934
                     /gene="ilvI"
                     /locus_tag="ACHUOF_004094"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR0613029.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            1937..2428
                     /gene="ilvN"
                     /locus_tag="ACHUOF_004095"
     CDS             1937..2428
                     /gene="ilvN"
                     /locus_tag="ACHUOF_004095"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0613030.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            2440..2526
                     /locus_tag="ACHUOF_004096"
     CDS             2440..2526
                     /locus_tag="ACHUOF_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR0613031.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            2608..3612
                     /gene="cra"
                     /locus_tag="ACHUOF_004097"
     CDS             2608..3612
                     /gene="cra"
                     /locus_tag="ACHUOF_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR0613032.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT          858 a          879 c         1019 g          920 t
ORIGIN      
        1 aaaatagaga aattgctgta agttgtggga ttcagccgat ttattatcaa tttaatcctt
       61 cgtaatggag gattttatcg tttcttttca cctttcctcc tgtttattct tattaccctg
      121 cgtttatgtc tctggctgcc aattgcttaa gcaagatcgg acggttaatg tgttttacac
      181 attttttccg tcaaacagtg aggcaggcca tggagatgtt gtctggagcc gagatggtcg
      241 tccgatcgct tatcgatcag ggcgttaaac aagtattcgg ttatcccgga ggcgcagtcc
      301 ttgatattta cgatgcattg cataccgtgg gtggtattga tcatgtatta gttcgtcatg
      361 agcaggcggc ggtgcatatg gccgatggcc tggcgcgtgc gaccggggaa gtcggcgtcg
      421 tgctggtaac gtcgggtcca agggcgacca atgcgattac cggcatcgcc accgcttata
      481 tggattccat tccattagtt gtcctttccg ggcaggtagc gacctcgttg ataggttacg
      541 atgcctttca ggagtgcgac atggtgggga tttcgcgacc ggtggttaaa cacagttttc
      601 tggttaagca aacggaagac attccgcagg tgctgaaaaa ggctttctgg ctggcggcaa
      661 gtggtcgccc aggaccagtg gtcgttgatt taccgaaaga tattcttaat ccggcgaaca
      721 aattacccta tgtctgccct gagtcggtca gtatgcgttc ttacaatccc actactaccg
      781 gacataaagg gcaaattaag cgtgctctgc aaacgctggt agcggcaaaa aaaccggttg
      841 tctacgtagg cggtggggca atcatggcgg gctgccatca gcagttgaaa gaaacggtgg
      901 aggcgttgaa tctgcccgtt gtttcctcat tgatgggaat gggggcgttt ccggcaacgc
      961 atcgtcaggc actgggcatg ctgggaatgc acggtaccta cgaagccaat atgacgatgc
     1021 ataacgcgga tgtgattttc gccgtcgggg tacgatttga tgaccgaacg acgaacaatc
     1081 tggcaaagta ctgcccaaat gccactgttc tgcatatcga tattgatcct acttccattt
     1141 ctaaaaccgt gactgcggat atcccgattg tgggggatgc tcgccaggtc ctcgaacaaa
     1201 tgcttgaact cctgtcgcaa gaatccgccc atcaaccgct ggatgagatc cgcgactggt
     1261 ggcagcagat tgaacagtgg cgcgctcttc agtgcctgaa atatgacact cacagtgaaa
     1321 agattaaacc gcaggcggtg atcgagactc tttggcggtt gactaatggg gacgcttacg
     1381 tgacgtccga tgtcgggcag caccagatgt ttgctgcgct ttattatcca ttcgacaaac
     1441 cgcgtcgctg gatcaattcc ggcggtctcg gcacgatggg ttttggttta cctgcggcac
     1501 tgggcgtcaa aatggcgttg ccagaagaaa ccgtggtttg cgtcactggc gacggcagta
     1561 ttcagatgaa catccaggaa ctgtctaccg cgttgcaata cgagttgccc gtactggtgg
     1621 tgaatctcaa taaccgctat ctggggatgg tgaaacagtg gcaggacatg atctattccg
     1681 gccgtcattc acaatcttat atgcaatcac tacccgattt cgtccgtctg gcggaagcct
     1741 atggtcacgt cgggatccag atctctcatc cgcatgagct ggaaagcaaa cttagcgagg
     1801 cgctggaaca ggtgcgcaat aatcgcctgg tgtttgttga tgttaccgtc gatggcagtg
     1861 agcacgtcta cccgatgcag attcgcgggg gcggaatgga tgaaatgtgg ttaagcaaaa
     1921 cggagagaac ctgattatgc gccggatatt atcagtctta ctcgaaaatg aatcaggcgc
     1981 gttatcccgc gtgattggcc ttttttccca gcgaggctac aacattgaaa gcctgaccgt
     2041 tgcgccaacc gacgatccga cattatcgcg tatgaccatc cagaccgtgg gcgatgaaaa
     2101 agtacttgag cagatcgaaa agcaattaca caagctggtc gatgtcttgc gcgtgagtga
     2161 gttggggcag ggcgcgcatg ttgagcggga aatcatgctg gtgaaaattc aggccagcgg
     2221 ttacgggcgt gacgaagtga aacgtaatac ggaaatattc cgtgggcaaa ttatcgatgt
     2281 caccccctcg ctttataccg ttcaattagc aggcaccagc gataagcttg atgcattttt
     2341 agcatcgatt cgcgatgtgg cgaaaattgt ggaagttgct cgctctggtg tggtcggact
     2401 ttcgcgcggc gataaaataa tgcgttgaga atgatctcag tgcgcaattt gcagcccaac
     2461 atgtcacgtt gggctttttt tgcgaaatca gtgggaacct ggaataaaag cagttgccgc
     2521 agttaatttt ctgcgcttag atgttaatga atttaaccca taccagtaca atggctatgg
     2581 tttttacatt ttacgcaagg ggcaattgtg aaactggatg aaatcgctcg gctggcggga
     2641 gtgtcgcgga ccactgcaag ctatgttatt aacggcaaag cgaagcaata ccgtgtgagc
     2701 gacaaaaccg ttgaaaaagt catggctgtg gtgcgtgagc acaattacca cccgaacgcc
     2761 gtggcagctg ggcttcgtgc tggacgcaca cgttctattg gtcttgtgat ccccgatctg
     2821 gagaacacca gctatacccg catcgctaac tatcttgaac gccaggcgcg gcaacggggt
     2881 tatcaactgc tgattgcctg ctcagaagat cagccagaca acgaaatgcg ctgcattgag
     2941 caccttttac agcgtcaggt tgatgccatt attgtttcga cgtcgttgcc acctgagcat
     3001 cctttttatc aacgctgggc taacgacccg ttcccgattg tcgcgctgga ccgcgccctc
     3061 gatcgtgaac atttcaccag cgtggttggt gccgatcagg atgatgccga aatgctggcg
     3121 gaagagttac gtaagtttcc cgccgagacg gtgctttatc ttggcgcgct gccggagctt
     3181 tctgtcagct tcctgcgtga acaaggtttc cgtactgctt ggaaagatga tccgcgcgaa
     3241 gtgcatttcc tgtatgccaa cagctatgag cgggaggcgg ctgcccagtt attcgaaaaa
     3301 tggctggaaa cgcatccgat gccgcaggcg ctgttcacaa cgtcgtttgc gttgttgcaa
     3361 ggagtgatgg atgtcacgct gcgtcgcgac ggcaaactgc cttctgacct ggcaattgcc
     3421 acctttggcg ataacgaact gctcgacttc ttacagtgtc cggtgctggc agtggctcaa
     3481 cgtcaccgcg atgtcgcaga gcgtgtgctg gagattgtcc tggcaagcct ggacgaaccg
     3541 cgtaagccaa aacctggttt aacgcgcatt aaacgtaatc tctatcgccg cggcgtgctc
     3601 agccgtagct aatccgcgaa caaaaatacg cgccaggtga atttccctct ggcgcgtaga
     3661 gtacgggact ggacat
//
LOCUS       DAZOYF010000263         3659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZOYF010000263 DAZOYF010000000
VERSION     DAZOYF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.269"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..273)
                     /locus_tag="ACHUOF_004098"
     CDS             complement(<1..273)
                     /locus_tag="ACHUOF_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR0613033.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
     gene            complement(286..2067)
                     /gene="gcl"
                     /locus_tag="ACHUOF_004099"
     CDS             complement(286..2067)
                     /gene="gcl"
                     /locus_tag="ACHUOF_004099"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFR0613034.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2157..2972)
                     /gene="allR"
                     /locus_tag="ACHUOF_004100"
     CDS             complement(2157..2972)
                     /gene="allR"
                     /locus_tag="ACHUOF_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFR0613035.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3050..3532)
                     /gene="allA"
                     /locus_tag="ACHUOF_004101"
     CDS             complement(3050..3532)
                     /gene="allA"
                     /locus_tag="ACHUOF_004101"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFR0613036.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
BASE COUNT          932 a         1032 c          839 g          856 t
ORIGIN      
        1 aatcgctgct gctacgccat cccgaaactc ttcttcacgg ccaggaatac aggcaatacc
       61 gcgctcgccc gccgcccagt cacctgccgg taaattgtgc agcgtatgtt cgagtttatt
      121 actcgccagt acctgtttta attcttcaat gtcgtagtca taaggaaaca taaattcaac
      181 gccgcgaaaa ccacactgcg cagctttctc aaaacgggcg agaaaatcat attcaccaaa
      241 taacatcgat aaattagcag agaaacgtaa catcatttat ctcccttatt catagtgcat
      301 gaagcaggtt tcagtcggtg catccgctgc gttatcggcg atatcttcaa attccatgac
      361 gttatccagt tcgctgccca tcgaaatatt ggtcacacgc tcgagaataa cttccacgac
      421 taccggtacc cgatattgcg ccattaaggc tttcgcctgt tcaaaggctg gcgcaatatc
      481 ttccggtttg aagacccgaa tagctttaca acctaaacct tccgctactt ttacgtggtc
      541 aacaccgtag ccattcactt cactggagtt gatattctcg aaagcgagtt gcacgcagta
      601 gtccatgtca aaagcgcgtt gtgactgacg aatcaggccg agataggcgt tattcaccag
      661 cacatggatg tacggaatgt tgaactgcgc gccaacggct aactcttcaa tcaggaactg
      721 gaagtcaaag tcgccagaaa tcgccaccac attgcgtttc ggatcagcgg cacaaccccc
      781 cagcgccgcc ggaatcgtcc agcctaacgg accagcctga ccacagttga tccagtggcg
      841 gtctttaaag acatgcagca tttgtgccgc agcgatttgt gacagaccaa tagtggtgac
      901 ataacaaaca tcgcgaccaa aggctttgtt catctcttca tacacgcgct gcggtttcac
      961 cggcacgttg tcgaagtggg ttttgcgcag caaagtgcgt ttacgctgct ggcagtcggc
     1021 gacccattct ttacgacacg gcagacgacc cgctttttgc atctcctgcg ccacttcaac
     1081 cagcagtgtc agcgccgctt tagcatcaga gacaatgccg agatccggac acagcacgcg
     1141 accaatttgc gtcggctcaa tatcaatatg aacgattttg cgcccttcgg tgtatttctc
     1201 taccgaaccg gtatgacggt tagcaaaacg gttaccgata ccaaacacca tgtcggacgc
     1261 cagcaacgtt gcgttaccgt aacgatgcgc ggtttgcaga ccgaccatcc cggccatcag
     1321 ttcatgatcg tccgggatac agccccagcc catcagcgtt gggatcaccg gaacgctggt
     1381 cagttcagca aactgttgta acagtgcagc tgcgtcagca ttgattaccc cgcccccggc
     1441 aacaatcacc ggacgttcgg cctggattaa catttctaca gctttttcga tctgcatacg
     1501 gctggcagca ggtttgtaga ccggcagcgg ttcgtacatg tcaggatcaa actcgatttc
     1561 cgcaacctga acgtcgaacg gtaaatccac cagtaccgga cccggacgac cagaacgcat
     1621 caggtgaaat gcctgttgca gcacgcgagg caccagcgcc gcttcacgaa ctgtaaccgc
     1681 cattttgctg accggtttag caattgcttc aatatctacg gcctgaaaat cttctttatg
     1741 cagacgggcg cgcggtgcct ggccggtaat gcacagaata ggaatggaat cagcagaagc
     1801 ggaatagagc gcggtgatca tgtccgtgcc cgcaggaccg gaagtcccca gacatacgcc
     1861 gatattccct gccgttgcgc gggtataacc ttccgccatg tgcgaaacac cttccacatg
     1921 acgcgccaga atgtgacgaa taccgccgtg cttacgcatc gctgagtaga acggattaat
     1981 tgcagctccc ggaacgccga aggcggtggt gataccttct ttctccagca catacattgc
     2041 cgcgtcaacg gctctcattt ttgccatttt attcctacct ctattagttg gaaaaatttt
     2101 ccaactttca aattttgtca aaaaaacccg ctccgcagag cgggatgcga cagacactat
     2161 ggatgtgctt tcagtcccaa cgccgtgctg atatcgcggg cggtgtctct gaccagctca
     2221 ccctgactga caaaacgatc ttctgtcagt cttgatgaag gcccggagat ggagatagcg
     2281 gcaacaactc taccgacatc atcgtaaatt gctgaagcta tgcaattcag acctacaaca
     2341 tgctcttctt tatctacggt atagcccagt tcacgcgctt gttccaggtc cttcagcaag
     2401 gtgggcatat ccacaagcgt agttggcgta aactgctgca aaccggtttg cagaatgatg
     2461 ctcatcaact cctcttccgc cagcggataa agcagcgctt tgcccgcacc ggaagcatgc
     2521 agcggcagac gactgcccag tggcgcacac atcctgacca tcgatttaca ctctaactga
     2581 ccaattaata ccgcttcatt gccgttacgg atcgcgacat tgaccgtttc gccggaaagt
     2641 aacatcaggc ggcgcataaa cggcccggcg acggagagga catcgcggtt atggatgtac
     2701 gccgcaccga cgttaaagac acctaatcct atatgccacc agcctaattg actgtcctga
     2761 tagacaaaat ccgctgcttg taaaaccttc agcaagcgaa aggtcgtgga gagcggcaaa
     2821 tccagattga gagaaatatc gctaaccgac gaacttcccc cacttttttc cagatattgc
     2881 agaatcgcaa ttccccgctc taacgcctgt gcgcccttct gtgccacagg ctccgcctgt
     2941 cctggcctgc cgcgccgtct aacttccgtc atacctacgt taactccgac ctacaagtga
     3001 gcataaacaa tgaacaatta gtttatctta tgcaagtcgg ttgcaggcgt cacgcaaaac
     3061 agagttcctg ttcaggaata ctttcaacat cacagttgtc actgccgccg cgatcgatgg
     3121 tcagaaaatc ggtgacgcgc tgccaggcga aaagtgggtg atgccagacg ttacgatggt
     3181 aattcacccc ctgttcaccg ttggtgataa acgcccgcag cgttgacagg tctggtttgt
     3241 cgtcgcctaa cgccacgacc accacaaaca cttcaccttt cattgggata aaggcctgaa
     3301 tgcccagcgg atgacgttcg agttcgtgaa tggtcagcgg cagattcgcc ggttgcgcgc
     3361 ggttaatgct gataagcgta cggtcttgct caagaatctc aaccagcgcc aaatcgtggt
     3421 aacgctccac caggccattg ttaatatgga aaaaatcccg ttgctgcgtt tcgattacgt
     3481 cgccataagc actaaaggct tcctgactta acggtaatac ctgaagtttc atctttttgt
     3541 ccttgtaaca taattccgtt caaccaccgc cgaccggtca gcttggtttc aataacaggc
     3601 aatctaaaac cttaagagaa caacattaat acaaaagttg gaatgttttt ccaaaacta
//
LOCUS       DAZOYF010000264         3592 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZOYF010000264 DAZOYF010000000
VERSION     DAZOYF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.270"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            62..1213
                     /gene="yiaY"
                     /locus_tag="ACHUOF_004102"
     CDS             62..1213
                     /gene="yiaY"
                     /locus_tag="ACHUOF_004102"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFR0613037.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            1378..2916
                     /gene="aldB"
                     /locus_tag="ACHUOF_004103"
     CDS             1378..2916
                     /gene="aldB"
                     /locus_tag="ACHUOF_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFR0613038.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3135..3278)
                     /locus_tag="ACHUOF_004104"
     CDS             complement(3135..3278)
                     /locus_tag="ACHUOF_004104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613039.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3461..>3592
                     /locus_tag="ACHUOF_004105"
     CDS             3461..>3592
                     /locus_tag="ACHUOF_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR0613040.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
BASE COUNT          894 a          859 c          942 g          897 t
ORIGIN      
        1 gttttcgaat gaaaatcctc agtaagctgc ccaccctttt ttacactttc aggagtgtgt
       61 tatggcatct tcaactttct ttattccttc tgtgaatgtc atcggcgctg attcattgac
      121 tgatgcaatg aatatgatgg cagattatgg atttacccgt accttaattg tcactgacaa
      181 tatgttaacg aaattaggta tggcgggtga tgtgcaaaaa gcactggaag aacgcaatat
      241 ttttagcgtt atttatgatg gcacccaacc taaccccacc acggaaaacg tcgccgcagg
      301 tttgaaatta cttaaagaaa ataattgcga tagcgtgatc tccttaggcg gtggttctcc
      361 acacgactgc gcaaaaggta ttgcgctggt ggcagccaat ggcggcgata ttcgcgatta
      421 cgaaggcgtt gaccgctctg caaaaccgca gctgccgatg atcgccatca ataccacggc
      481 gggtacggcc tctgaaatga cccgtttctg catcatcact gacgaagcgc gtcataacaa
      541 aatggcgatt gttgataagc acgtgactcc gctgctttct gtcaatgact cctctctgat
      601 gattggtatg ccgaagtcac tgaccgccgc aacgggtatg gatgccttaa cgcacgctat
      661 cgaagcgtat gtttctattg ccgccacgcc gatcactgac gcttgtgcac tgaaagccgt
      721 gaccatgatt gccgaaaacc tgccgttagc cgttgaagat ggcagtaatg cgaaagcgcg
      781 tgaagcaatg gcttatgccc agttccttgc cggtatggcg ttcaataatg cttctctggg
      841 ttatgttcat gcgatggcgc accagctggg cggtttctac aacctgccac acggtgtatg
      901 taacgccgtt ctgctgccgc atgttcaggt attcaacagc aaagtcgccg ctgcacgtct
      961 gcgtgactgt gccgctgcaa tgggcgtgaa cgtgacaggt aaaaacgacg cggaaggtgc
     1021 tgaagcctgc attaacgcca tccgtgaact ggcgaagaaa gtggatatcc cggcaggcct
     1081 acgcgacctg aacgtgaaag aagaagattt cgcggttctg gcgactaatg ccctgaaaga
     1141 tgcctgtggc tttactaacc cgatccaggc aactcacgaa gaaattgtgg tgatttatcg
     1201 cgccgcaatg taatcatcat tccaacaacg gctggcaaat tgtcagccgc tttttcaact
     1261 atctctgtaa tccttgcccg taaattcgtg atagctgtcg taaagctgtt accgactggc
     1321 gaagatttcg ccagtcacgt ctacccttgt tatacctcac accgcaagga gacgatcatg
     1381 accaataatc ccccttcagc acagattaag cccggcgagt atggtttccc catcaagtta
     1441 aaagcccgct acgacaactt tattggcggt gaatgggtag cccctgccga tggtgagtat
     1501 taccagaatc tgacgccggt gaccgggcag ctgctgtgcg aagtggcgtc ttcgggcaaa
     1561 cgagacatcg atctggcgct ggatgctgcg cacaaagtga aagataaatg ggcgcacacc
     1621 tcggtgcagg atcgcgcggc gattctgttt aagattgccg atcgaatgga acaaaacctc
     1681 gagctgttag cgacagctga aacctgggat aacggcaaac ccattcgcga aaccagtgct
     1741 gcggatgtac cgctggcgat tgaccatttc cgctatttcg cctcgtgtat tcgggcgcag
     1801 gaaggtggga tcagtgaagt tgatagcgaa accgtggcct atcatttcca tgaaccgtta
     1861 ggcgtggtgg ggcagattat cccgtggaac ttcccgctgc tgatggcgag ctggaaaatg
     1921 gctcccgcgc tggcggcggg caactgtgtg gtgctgaaac ccgcacgtct taccccgctt
     1981 tctgtactgc tgctaatgga aattgtcggt gatttactgc cgccgggcgt ggtgaacgtg
     2041 gtcaatggcg caggtgggga aattggcgaa tatctggcga cctcgaaacg catcgccaaa
     2101 gtggcgttta ccggctcaac ggaagtgggc caacaaatta tgcaatacgc aacgcaaaac
     2161 attattccgg tgacgctgga gttgggcggt aagtcgccaa atatcttctt tgctgatgtg
     2221 atggatgaag aagatgcctt tttcgataaa gcgctggaag gctttgcact gtttgccttt
     2281 aaccagggcg aagtttgcac ctgtccgagt cgtgccttag tgcaggagtc aatctacgaa
     2341 cgctttatgg aacgcgccat ccgccgtgtc gaaagcattc gcagcggtaa cccgctcgac
     2401 agcgtgacgc aaatgggcgc gcaggtttct cacgggcaac tggaaaccat cctcaactac
     2461 attgatatcg gtaaaaaaga gggcgctgac gtgctcacag gcgggcggcg caagctgctg
     2521 gaaggtgaac tgaaagacgg ctactacctc gaaccgacga ttctgtttgg tcagaacaat
     2581 atgcgggtgt tccaggagga gatttttggc ccggtgctgg cggtgaccac cttcaaaacg
     2641 atggaagaag cgctggagct ggcgaacgat acgcaatatg gcctgggcgc gggcgtctgg
     2701 agccgcaacg gtaatctggc ctataagatg gggcgcggca tacaggctgg gcgcgtgtgg
     2761 accaactgtt atcacgctta cccggcacat gctgcgtttg gtggctacaa acaatcaggt
     2821 atcggtcgcg aaacccacaa gatgatgctg gagcattacc agcaaaccaa gtgcctgctg
     2881 gtgagctact cggataaacc gttggggctg ttctgatata agaagctggt cgcattgggt
     2941 attcattgcc tgatgcgacg cttacgcgtc ttatcatgcc tacgggaacc tgaccgtagg
     3001 ccggataagg cgtacacgcc gcatccggca taaacaaagc gcacgttgtt acaatttacg
     3061 ttttaaacct acgtgcgcaa taacccagac tacgccaaca gaagtgcgtt tctggggcgt
     3121 gttttgtcag gagtttaatc tcgcgtttcg atggtgagcg ggcgaaccag gccgtaaatc
     3181 accattggtg atccgaggcc aatcagcaat aaacggtaga taattgaagg tttaatacgc
     3241 ttaatctgta ctgtggatgt tttactggag ttattcatta gtttggccaa tagttccact
     3301 atttcaatat gataaacctc gtcctgttga aaaaccagac agcacatttc agaaagtata
     3361 taaaaaacaa tatgtcgtta tataaataag gaaattgcac tgcatcatgg catttcataa
     3421 tatgcgttaa ttgttctttt ttatattctt tgagggaaat atgttcctgg actattttgc
     3481 actgggagtg cttatttttg tatttctggt gatattctat gggattatta ttttacatga
     3541 tattccctac ctgattgcta aaaaacgtaa tcatcctcat gccgacgcca tt
//
LOCUS       DAZOYF010000265         3548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZOYF010000265 DAZOYF010000000
VERSION     DAZOYF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.271"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="ACHUOF_004106"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="ACHUOF_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR0613041.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="ACHUOF_004107"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="ACHUOF_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR0613042.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHUOF_004108"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHUOF_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR0613043.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIKNYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          772 c          819 g          951 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tttataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAZOYF010000266         3537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZOYF010000266 DAZOYF010000000
VERSION     DAZOYF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.272"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            49..438
                     /locus_tag="ACHUOF_004109"
                     /pseudo
     CDS             49..438
                     /locus_tag="ACHUOF_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(416..1039)
                     /gene="tetR(B)"
                     /locus_tag="ACHUOF_004110"
     CDS             complement(416..1039)
                     /gene="tetR(B)"
                     /locus_tag="ACHUOF_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR0613044.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1121..2326
                     /gene="tet(B)"
                     /locus_tag="ACHUOF_004111"
     CDS             1121..2326
                     /gene="tet(B)"
                     /locus_tag="ACHUOF_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR0613045.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2439..3032)
                     /gene="tetC"
                     /locus_tag="ACHUOF_004112"
     CDS             complement(2439..3032)
                     /gene="tetC"
                     /locus_tag="ACHUOF_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFR0613046.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3120..3506
                     /locus_tag="ACHUOF_004113"
                     /pseudo
     CDS             3120..3506
                     /locus_tag="ACHUOF_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          967 a          650 c          724 g         1196 t
ORIGIN      
        1 gggcattatc tgaacataaa acactatcaa taagttggag tcattaccaa ccatggcgta
       61 catgccaaag tttccacgcc agtgcattta cgaaaagcac gtacttgcta tgatcattta
      121 gctggcgaag ttgccgttaa gatctatgat tccctttgtc aacagcaatg gatcactgaa
      181 aatggttcaa tgatcacatt aagtggtatt caatattttc atgaaatggg aattgacgtt
      241 ccttccaaac attcacgtaa aatctgttgt gcgtgtttag attggagtga acgccgtttc
      301 catttaggtg ggtacgttgg agccgcatta ttttcgcttt atgaatctaa agggtggtta
      361 actcgacatc ttggttaccg tgaagttacc atcacggaaa aaggttatgc tgcttttaag
      421 acccactttc acatttaagt tgtttttcta atccgcatat gatcaattca aggccgaata
      481 agaaggctgg ctctgcacct tggtgatcaa ataattcgat agcttgtcgt aataatggcg
      541 gcatactatc agtagtaggt gtttcccttt cttctttagc gacttgatgc tcttgatctt
      601 ccaatacgca acctaaagta aaatgcccca cagcgctgag tgcatataat gcattctcta
      661 gtgaaaaacc ttgttggcat aaaaaggcta attgattttc gagagtttca tactgttttt
      721 ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg atgacttagt aaagcacatc
      781 taaaactttt agcgttatta cgtaaaaaat cttgccagct ttccccttct aaagggcaaa
      841 agtgagtatg gtgcctatct aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat
      901 tttttacatg ccaatacaat gtaggctgct ctacacctag cttctgggcg agtttacggg
      961 ttgttaaacc ttcgattccg acctcattaa gcagctctaa tgcgctgtta atcactttac
     1021 ttttatctaa tctagacatc attaattcct aatttttgtt gacactctat cattgataga
     1081 gttattttac cactccctat cagtgataga gaaaagtgaa atgaatagtt cgacaaagat
     1141 cgcattggta attacgttac tcgatgccat ggggattggc cttatcatgc cagtcttgcc
     1201 aacgttatta cgtgaattta ttgcttcgga agatatcgct aaccactttg gcgtattgct
     1261 tgcactttat gcgttaatgc aggttatctt tgctccttgg cttggaaaaa tgtctgaccg
     1321 atttggtcgg cgcccagtgc tgttgttgtc attaataggc gcatcgctgg attacttatt
     1381 gctggctttt tcaagtgcgc tttggatgct gtatttaggc cgtttgcttt cagggatcac
     1441 aggagctact ggggctgtcg cggcatcggt cattgccgat accacctcag cttctcaacg
     1501 cgtgaagtgg ttcggttggt taggggcaag ttttgggctt ggtttaatag cggggcctat
     1561 tattggtggt tttgcaggag agatttcacc gcatagtccc ttttttatcg ctgcgttgct
     1621 aaatattgtc gctttccttg tggttatgtt ttggttccgt gaaaccaaaa atacacgtga
     1681 taatacagat accgaagtag gggttgagac gcaatcgaat tcggtataca tcactttatt
     1741 taaaacgatg cccattttgt tgattattta tttttcagcg caattgatag gccaaattcc
     1801 cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga tggaatagca tgatggttgg
     1861 cttttcatta gcgggtcttg gtcttttaca ctcagtattc caagcctttg tggcaggaag
     1921 aatagccact aaatggggcg aaaaaacggc agtactgctc ggatttattg cagatagtag
     1981 tgcatttgcc tttttagcgt ttatatctga aggttggtta gttttccctg ttttaatttt
     2041 attggctggt ggtgggatcg ctttacctgc attacaggga gtgatgtcta tccaaacaaa
     2101 gagtcatcag caaggtgctt tacagggatt attggtgagc cttaccaatg caaccggtgt
     2161 tattggccca ttactgtttg ctgttattta taatcattca ctaccaattt gggatggctg
     2221 gatttggatt attggtttag cgttttactg tattattatc ctgctatcga tgaccttcat
     2281 gttaacccct caagctcagg ggagtaaaca ggagacaagt gcttagttat ttcgtcacca
     2341 aatgatgtta ttccgcgaaa tataatgacc ctcttgataa cccaagaggg cattttttac
     2401 gataaagaag atttagcttc aaataaaacc tatctatttt atttatcttt caagctcaat
     2461 aaaaagccgc ggtaaatagc aataaattgg ccttttttat cggcaagctc ttttaggttt
     2521 ttcgcatgta ttgcgatatg cataaaccag ccattgagta agtttttaag cacatcatca
     2581 tcataagctt taagttggtt ctcttggatc aatttgctga caatggcgtt taccttacca
     2641 gtaatgtatt caaggctaat tttttcaagt tcattccaac caatgatagg catcacttct
     2701 tggataggga taaggttttt attattatca ataatataat caagataatg ttcaaatata
     2761 ctttctaagg cagaccaacc atttgttaaa tcagtttttg ttgtgatgta ggcatcaatc
     2821 ataattaatt gctgcttata acaggcactg agtaattgtt ttttattttt aaagtgatga
     2881 taaaaggcac ctttggtcac caacgctttt cccgagatct catctattga aacagcttga
     2941 tagccttttt caacaaacaa tattcgtgct gagttaacca gtgattgata ggtactctta
     3001 aaattttctt gttgatgatt tttattttcc atgatagatt taaaataaca taccgtcagt
     3061 atgtttatgg tatcatgatg atgtggtcgt gacaatctta agaacattta ggttatttta
     3121 tgtatattga acagcattct cgctatcaaa ataaagctaa taacatccaa ttaagatatg
     3181 atgataagca gtttcataca acggttatca aagatgttct attatggatt gaacataatt
     3241 tagatcagtc tttactgctt gatgatgtgg cgaataaagc gggttatacc aagtggtatt
     3301 ttcagcggct gttcaaaaaa gtaacagggg tcacactggc tagctatatt cgtgctcgtc
     3361 gtttgacgaa agcggctgtt gagttgaggt tgacgaaaaa aactatcctt gagatcgcat
     3421 taaaatatca atttgattcc caacaatctt ttacacgtcg atttaagtac atttttaagg
     3481 ttacaccaag ttattatcgg cgtaataaat tatgggaatt ggaggcaatg cactgag
//
LOCUS       DAZOYF010000267         3498 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZOYF010000267 DAZOYF010000000
VERSION     DAZOYF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.273"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..312
                     /locus_tag="ACHUOF_004114"
     CDS             <1..312
                     /locus_tag="ACHUOF_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFR0613047.1"
                     /translation="ALDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYC
                     QYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASM
                     A"
     gene            469..1218
                     /locus_tag="ACHUOF_004115"
     CDS             469..1218
                     /locus_tag="ACHUOF_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR0613048.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1240..1806
                     /gene="yieF"
                     /locus_tag="ACHUOF_004116"
                     /gene_synonym="chrR"
     CDS             1240..1806
                     /gene="yieF"
                     /locus_tag="ACHUOF_004116"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR0613049.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1860..3197)
                     /gene="adeP"
                     /locus_tag="ACHUOF_004117"
     CDS             complement(1860..3197)
                     /gene="adeP"
                     /locus_tag="ACHUOF_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR0613050.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3362..>3498
                     /locus_tag="ACHUOF_004118"
     CDS             3362..>3498
                     /locus_tag="ACHUOF_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR0613051.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     R"
BASE COUNT          961 a          908 c          865 g          764 t
ORIGIN      
        1 gcactggaca atgtgttaca ggagctgggg cgcgaacgca cgattgctat gagcctgccg
       61 gaattcgagc agtcactgtt tatggcagcg caacccgaca atctgctact ggcgaccgcg
      121 ccgcgctact gtcagtacta caatcaactc catcaactgc cgttggttgc tcttcctctc
      181 ccgtttgacg aaagccagca aaaaaagctg gaagttcctt ttaccctgct gtggcataaa
      241 cggaacagcc ataatccgaa gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg
      301 tcgatggcat aaccgaatcg tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag
      361 ttgtaataag cttgtctgaa tcgaactttt agccgcttta gtctgtccat cattccagta
      421 aatgattact cttgtattca taatggacca ttaagcatgg agcgaaaaat ggcgactcac
      481 tttgcccgag ggattttaac ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt
      541 catcaagaag cccgaaacat tccccctcat cgtcaaagcc gttttctggc gtccagaggt
      601 ttactcgcag aactgatgtt catgctgtat ggcattggcg aattgccgga aatcgtcacc
      661 ctgccgaaag gtaaaccggt tttcagtgat aaaaatttgc cttcgttttc tatttcctat
      721 gccgggaata tggttggcgt ggcgttaaca accgaaggtg aatgtggcct cgatatggaa
      781 ctacagcgtg cgacgcgcgg gtttcatagc ccacacgcgc ccgataacca caccttttcc
      841 agcaatgaat cgctatggat cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc
      901 attacgctgc gccgaagcgt gctaaaacta accggtgatg ttttgaatga cgatccgcgc
      961 gatctgcagc tgctgcccat tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa
     1021 gcgttatgcg acgcggagga cgtgctggtg tggtccgtgg cggtcacgcc cgcgattgaa
     1081 aagctcagtg tctgggagtt agatggcaaa cacagctgga aaagcctgcc ggatattcac
     1141 agccgcgcca acaatcctac cagccggatg atgcgttttg cccagctctc taccgtgaag
     1201 tctttttcgc caaattgata gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg
     1261 gttacgttac tggggagcct gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg
     1321 ccgaaaattg ctccggcgag catggaagtc aatgcgttac cttccattgc cgacattccc
     1381 ctgtatgacg ctgacgtaca gcaggaagaa ggttttccag caacggttga agctctggcg
     1441 gaacagatcc gtcaggctga cggtgtggtg atcgtcacgc cggaatataa ctactcggta
     1501 ccgggtgggc tgaaaaatgc catcgactgg ctttcccgcc tgccggatca accgctggcc
     1561 ggtaaaccgg tgttgattca gaccagctca atgggcgtga ttggcggcgc gcgctgtcag
     1621 tatcacctgc gccagattct ggttttcctc gatgcaatgg tgatgaacaa gccggaattt
     1681 atgggcggcg tgattcagaa caaagttgat ccgcaaaccg gagaagtgat tgatcagggt
     1741 acgctggacc acctgaccgg gcaattgacc gcatttggtg agtttattca gcgagttaag
     1801 atctaaataa aaaacccgcc agtaatcatg catggcgggt ttttaatgcg ctatcgattt
     1861 taatgagcgt cgataaatac aatcttcagg ataaacagca gcgcaacgat gattacgcac
     1921 gggctaaggt cacgcagacg cccggtacca atcttcatca cgcagtagga gataaagccc
     1981 agcgcaatac cttcggtaat cgaaaagctg aacggcatca tcacggcggt aataaacgcc
     2041 ggaacagatt cagtaagatc ctgccagttc acgcgagcaa gacttgaggt catcaacacg
     2101 ccaacgtaga tcaacgcgcc agctgcagcg tagtctggca ccattcccgc cagcggcgac
     2161 agaaagataa ccagcaggaa cagcagacca acaaccactg ccgtcagacc ggtacgaccg
     2221 ccaaccgata cgccggaaga ggactcaata taagccgtaa cggaagaagt accgataaac
     2281 gaaccggtca cggaagagat actgtcgaca tacagcgcct gcttcatgcg cgggaatttc
     2341 cccttctcat ccgccagacc tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca
     2401 aacaagttga ccaacatgaa agagaaaatc acccctgcca gcccgaggtt aaacgacccg
     2461 gctaaatcta catgacccac aactgtcatt acgctcggcg gcgcagaaac gatgccattg
     2521 tagtgcacat cacccagcat ccagcccagc agcgtcgtca ccacgataga aaccagcacc
     2581 gctgcgtgaa tgttgcgcga ggccagaata gcaatgatga aaaagccgag gatacccaga
     2641 agtacgctgt gagaagtcag attaccgatg ctcaccagcg tttccgagtt agcgacaatc
     2701 acacctgcgt ttttcagccc catcatgccg ataaacaggc cgataccgct ggtaataccc
     2761 acacgcagac ttaccggaat gttagcaatc atccagtaac gaacgcggaa aatggtcaaa
     2821 agaagcagtc cgacagcgcc ccagaagatt gcgcccatac cgatctgcca cggcagcccc
     2881 attgcctgga caaccacaaa agcgaaaaag gcgttaagcc ccatcgcagg agccagcgcc
     2941 accggaaggt ttgcaaacaa tcccatcata atgctaccga aagcagcaat cagacaggtt
     3001 gttacgaaga cggcactggt gtccatgcca gcaacgccca gaatttgtgg gttaacaaat
     3061 acgatgtaga ccatcgtcag gaaggtggtg aacccggcaa tcacttcagt tcgtgccgta
     3121 gtaccatgtt cgcgcagttt aaaaacacgt tccagcatcc tctgaccaga agtctgggtg
     3181 gtatgttgat gactcattat ctatttccga acaaggaggg aaaatccgtc gctatcgtat
     3241 accaaaatgc gacaataggc gcgtttgtga gagacttttt tattggattt acttatacgg
     3301 caacgattgc gttgcgcaaa tcggctctac gaaaacgtta aactgattaa aaaggaaagg
     3361 catgtcccgg atagaagcgg tatttttcga ctgcgacggt acgctggtcg acagtgaagt
     3421 catttgctcc cgcgcatatg taacgatgtt tcaggaattt ggtattacgc tcgatcctga
     3481 agaggtattc aaacgttt
//
LOCUS       DAZOYF010000268         3456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZOYF010000268 DAZOYF010000000
VERSION     DAZOYF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.274"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(226..429)
                     /locus_tag="ACHUOF_004119"
     CDS             complement(226..429)
                     /locus_tag="ACHUOF_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613052.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="ACHUOF_004120"
     CDS             complement(431..586)
                     /locus_tag="ACHUOF_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0613053.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="ACHUOF_004121"
     CDS             complement(789..1196)
                     /locus_tag="ACHUOF_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0613054.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="ACHUOF_004122"
     CDS             1273..1500
                     /locus_tag="ACHUOF_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR0613055.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="ACHUOF_004123"
     CDS             1484..1906
                     /locus_tag="ACHUOF_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR0613056.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="ACHUOF_004124"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="ACHUOF_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3456
                     /locus_tag="ACHUOF_004125"
                     /pseudo
     CDS             2768..3456
                     /locus_tag="ACHUOF_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          885 a          764 c          940 g          867 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggt
//
LOCUS       DAZOYF010000269         3414 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZOYF010000269 DAZOYF010000000
VERSION     DAZOYF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.275"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..238
                     /locus_tag="ACHUOF_004126"
     CDS             <1..238
                     /locus_tag="ACHUOF_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFR0613057.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="ACHUOF_004127"
     CDS             248..886
                     /locus_tag="ACHUOF_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR0613058.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="ACHUOF_004128"
     CDS             889..2052
                     /locus_tag="ACHUOF_004128"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0613059.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3414
                     /locus_tag="ACHUOF_004129"
     CDS             2136..>3414
                     /locus_tag="ACHUOF_004129"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR0613060.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          760 a          803 c          987 g          864 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggtt
//
LOCUS       DAZOYF010000270         3413 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZOYF010000270 DAZOYF010000000
VERSION     DAZOYF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.276"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            75..791
                     /gene="nanC"
                     /locus_tag="ACHUOF_004130"
     CDS             75..791
                     /gene="nanC"
                     /locus_tag="ACHUOF_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR0613061.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            811..1917
                     /gene="nanM"
                     /locus_tag="ACHUOF_004131"
     CDS             811..1917
                     /gene="nanM"
                     /locus_tag="ACHUOF_004131"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR0613062.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1982..2962
                     /locus_tag="ACHUOF_004132"
     CDS             1982..2962
                     /locus_tag="ACHUOF_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR0613063.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3072..3277
                     /locus_tag="ACHUOF_004133"
                     /pseudo
     CDS             3072..3277
                     /locus_tag="ACHUOF_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT          995 a          726 c          801 g          891 t
ORIGIN      
        1 attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca
       61 aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt
      121 cgtccccatt aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa
      181 gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa
      241 gcatggaaag taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca
      301 atgatgttca ggttgaagtt aattacgcaa ttaaacttga tgatcaatgg acggtgcgcc
      361 cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt
      421 cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt
      481 accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat
      541 atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca
      601 aacagaatga ttatcgctat gcaaaccaca agaaatgggc gacggaaaat gcctttgttc
      661 tacaatacca tatgacgccc gatattacgc catacataga atatgactac cttgaccgcc
      721 agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat
      781 ttaaactgta gtagacagga gacagtcaca atgaataaaa ctataatggc gcttgctatc
      841 atgatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa
      901 agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtaca
      961 gcatggtaca agctagatac acaggccaaa gataaaaaat ggactgcgtt agctgcattc
     1021 cctggtggac cgagagagca agcaacctct gcatttattg atggcaatct gtatgtgttt
     1081 ggcggcattg gcaaaaacag cgagggattg actcaggtat ttaatgacgt acacaaatac
     1141 aaccccaaaa ccaatagctg ggttaaattg atgtcgcacg cgccgatggg catggcgggt
     1201 catgtgactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc
     1261 ttcaatggct attttgaaga tctcaacgag gctggaaaag attcaaccgc tatagataaa
     1321 atcaacgccc actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg
     1381 tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg
     1441 gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa
     1501 ccaggattgc gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg
     1561 aataagcttg atcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc
     1621 aatgattctc ttatatttgc tggaggggcc ggattcaaag gttcacgaga aaattaccag
     1681 aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt
     1741 tggcataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta
     1801 tcattgccct ggaataatag tttattaatt attggcggtg aaactgcagg cggcaaagcg
     1861 gtgacggatt cagttttaat ttctgtgaag gataataaag tcacagtaca aaactaacgc
     1921 ttcagggccc cggtaaaggg gccctgctat caatttgtca tttgtattac gcgaaaatta
     1981 tatgaacgca ataatatcac ccgattatta ctatgttctt accgttgctg gtcagtctaa
     2041 tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc ctcattccag
     2101 aattaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgccat gtcactttaa
     2161 cgacattatt ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca
     2221 tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc
     2281 acggaaatta ctgcccttca ttcctgataa tgcagggatt cttatcgttc cgtgttgccg
     2341 tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag
     2401 ccatgatgct tgtcgttggg gaacggatac tccgctgtac caggatttag tcagcagaac
     2461 gcgagccgca ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct ggatgcaagg
     2521 cgaatttgac ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt
     2581 tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc
     2641 gccgtggttt tgcggcgata ccacctggta ctggaaagaa aatttcccgc attcgtatga
     2701 agctatttat ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca
     2761 gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaagcac
     2821 gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag
     2881 tcatttcagc acggcagccc gtcgggggat tatttctgac agatttgtag aagcaatttt
     2941 gcagttttgg cgcgaaaggt gagcggggag tttattcatg ggggatgtcc ttgttcgttc
     3001 tcagagtaga caacaaaaaa caaatgagat caaggtaata tctcttcact tgcgggctcg
     3061 aacagcaggc aattgccaat ataataatct ggcacaaggg tgaaaaacgc gcaccgcata
     3121 aaccgttgtt attgcttaca gattagccgg atgccttaat agacatccgc gccttttcga
     3181 ttatggctcg gaaatctacg aacatctaca cagtttactg gaacgttttg ggccacagcg
     3241 ttcgcagcac cgtcctgata tgccgttctg gcgattataa gtcgacggat tctggcaaat
     3301 agacgatgat gatatgctta atgatgttca aagaaattag gccattatta tattattgct
     3361 tgcattttct ttcattccta aaagcacccg ccattaatcc caggcgcatt atg
//
LOCUS       DAZOYF010000271         3373 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZOYF010000271 DAZOYF010000000
VERSION     DAZOYF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.277"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..1202
                     /locus_tag="ACHUOF_004134"
     CDS             <1..1202
                     /locus_tag="ACHUOF_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR0613064.1"
                     /translation="TKDGQRLRNMIMSFNADIVARSPKKKPTFFPEQIEGYEYMYGGN
                     DDYPYYLQNRTDENGNDLPIGPISYMENPEVPQANAYMLEAATNAVKEVASLGVDAQA
                     ANGQVAFDTVNQLNMRADLETYVFQDNLATAMRRDGEIYASMVNDIYDVPRHVTLTLE
                     DGSEKDVQLYAQVVDYQSGNVVTLNDIRGRYECYTDVGPSFQSMKEQNRAEIQELLTK
                     VPQGTPEFQMLMLQYFTLLDGKGVEMMREYANKQLVMMGLKKPETPEEMEMVQQAQQQ
                     PQQPSAEQIQAQGILLQGQAELLKAENQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMD
                     LDKQAELREYLRLVGQFQQQRSKDARANAELLLKDADQTHSQRMDFANLMRQVQIPSG
                     GVAETPQ"
     gene            1216..2127
                     /locus_tag="ACHUOF_004135"
     CDS             1216..2127
                     /locus_tag="ACHUOF_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFR0613065.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            2127..>3373
                     /locus_tag="ACHUOF_004136"
     CDS             2127..>3373
                     /locus_tag="ACHUOF_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFR0613066.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACA"
BASE COUNT          927 a          872 c          872 g          702 t
ORIGIN      
        1 tgacgaaaga cggtcaacgc cttcgtaaca tgattatgtc gttcaacgcc gatattgttg
       61 ctcggtcacc gaagaagaaa ccgaccttct tccctgagca aatcgaaggc tacgaataca
      121 tgtacggcgg aaatgatgac tatccgtact atctgcagaa caggactgat gaaaacggta
      181 acgacctgcc gattggtcca atctcctaca tggaaaaccc tgaagtgccg caagccaacg
      241 cttacatgct tgaggctgcc accaacgcag tgaaagaagt ggctagtctt ggcgtggatg
      301 cgcaggcggc aaatggtcag gtcgcttttg ataccgtcaa tcaactgaac atgcgggcag
      361 accttgagac atacgtgttt caggataacc tggctaccgc aatgcgacgt gatggcgaga
      421 tttatgcctc aatggtcaac gatatttatg acgttcctcg tcatgtaacg ctgacacttg
      481 aagatggaag cgagaaagac gttcaactct atgcgcaagt tgtcgattac cagtccggca
      541 atgtggtcac actcaacgac attcgcggtc gatatgagtg ctatacagac gttgggccat
      601 ccttccagag tatgaaggaa cagaaccgcg cagagattca ggagttgctc accaaggttc
      661 cgcaaggtac tccagagttc cagatgctga tgctgcaata cttcacgctg cttgacggta
      721 aaggcgtcga gatgatgcga gagtacgcga acaagcaact ggtgatgatg gggctgaaga
      781 aaccagaaac acctgaagag atggagatgg tgcagcaggc acaacaacag ccgcagcagc
      841 catcagcaga gcaaattcag gcgcagggta tccttctgca aggtcaggct gaattgctca
      901 aggcagagaa ccaacaggcg cagattcagg ttgaagctgc caaggttgaa gcccaaaacc
      961 aactcaacgc cgcgaagatt gcagaaatat tcaacaatat ggacctcgac aagcaggcag
     1021 aactgcgtga gtacctcagg ctcgtaggtc aattccagca acagcgcagc aaagatgctc
     1081 gtgctaacgc tgagctgctt cttaaagatg cagaccagac tcattcacaa cgcatggatt
     1141 tcgcgaatct tatgcgtcaa gttcaaatcc cctccggcgg agtagccgag acacctcaat
     1201 aagagagagt taatcatgga ccaaaccacc gacattcagg cttctgaaga attaaccctg
     1261 cccggcaatc atgcagcggc atctgctgat ggcttagttg tcgataatgc caacgacagc
     1321 gcaggtcagg aagaaggctt tgagattgtc cggaaagacg atgagaaacc aaaacaagac
     1381 ccggcaacta atgctgaatt tgcccgtcgc cgcatcgaac gcaaacgcca gcgtgagctt
     1441 gagcagcaga tggaagcggt taagcgtgga gagttgccgg agcacctgcg ggtgaaccct
     1501 gagttaccaa aacaaccaga ccctaacgat tatctttccg aagatgcact ggctaagtac
     1561 gactatgacc agagccgcgc actggctgcc ttccagcagg caaacagtga atggcagatc
     1621 aaggctatgg acgcacgaag ccaggctgtc gccgagcagg gtcgcaaaac tcaggagttc
     1681 acccagcaat cagcgcaata cgtcgaggcg gcccgtaagc actacgaggc agcggaaaag
     1741 ctcaatatcc ctgactatca ggagaaagag gatgcattca tgcaactggt gccgccagca
     1801 gtcggtgccg acatcatgcg cctcttcccg gagaaatccg ccgctctcat gtatcacctt
     1861 ggtgctaatc ctgagaaaac acgccagttg ctggcgatgg acgggcaatc cgcgctgatt
     1921 gaactcactc gactgtcaga acgtttaact ctcaagcctc gagccaagcc tgtttcagaa
     1981 gccccgttac ctgatgaacc cattcaggga catgctgttg ctgcaaatat ctctgcgatt
     2041 gaaaagcaga tggaggcggc agcaaacaaa ggggatgtag agacgtaccg caagctcaag
     2101 gcgcaactga ataaaggaat tcgataatgg cattaaatga aggtcaactg gtcacgtatg
     2161 ctctggatga aatcatcgga accgtccaga acctgacgcc aatggcgtca aaagtgacaa
     2221 aatacccccc tccggcagaa tccatgcagc gttcaagcaa caccgtgtgg atgcctgttg
     2281 agcaggaagc gccaacccag actggctggg atttaactgg caacgcaacc gggattctgg
     2341 aactctccgt gaagtgcaac atgggcgatc cggataacga tttcttcgag cttcgtgcag
     2401 atgacctgcg tgatgagcgt tcttaccgtc gccgcatcca ggcatccgcc aaaaaactgg
     2461 cgaataacat tgagtcagcg attgccaaac aggcaactga aatgggctcg cttgttgttc
     2521 acgatacccg cgcaattggt ccatcgactg gcctgcctgg ctgggatttt gtgtctgatg
     2581 cagagcgact gatgttctcc cgtgagctaa accgcgacat gggcatcagt tacttcctga
     2641 accctgacga ttaccgcaaa gcaggccgca acctggtaga tggtgacatc ttcgggcgcg
     2701 ttcctgaaga agcatatcga aacggtacta ttcagcgtca gattgctggc tttgatgaaa
     2761 ttcttcgctc accgaaactt ccggcagtta ccaagtcaac cgcgactggt gtaactgttt
     2821 ctggtgcgca gaagtttaag ccgcaggcat acacccttga taccgatggt aacaaagaga
     2881 acgtcgacaa ccgtgttgca acggtgaccg tatcctccac caccggattt aagcgcggcg
     2941 acaaaatcag cttcattggt gtgaagttcc tgtctcagat ggcgaagaat gtgctgactg
     3001 atgacgcgac tttctcaatc acccgtgtga tcgatggtac tcacatcgaa atcacgccga
     3061 agccgattgc gctggatgac gcgtcactga caaaagaaga gaaggcttac gctaacgtaa
     3121 acacctctct tgctgatacc actccggtaa acgttctgaa cgtggcaaca accaccgcta
     3181 acgtgttctg ggctgatgac tcaatccgcc tgctgtctca gccgatcccg gtaacccatg
     3241 aactgtttgc tggcatgaaa acgtcttcct tcagcattcc aggtattggt gttaacggca
     3301 tcttcgcaac gcagggtgat atcaacactc tgtctggtaa gtgccgtatt gctgtgtggt
     3361 attcagcatg tgc
//
LOCUS       DAZOYF010000272         3349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZOYF010000272 DAZOYF010000000
VERSION     DAZOYF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.278"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..224
                     /locus_tag="ACHUOF_004137"
     CDS             <1..224
                     /locus_tag="ACHUOF_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR0613067.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="ACHUOF_004138"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="ACHUOF_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR0613068.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="ACHUOF_004139"
     CDS             518..1108
                     /locus_tag="ACHUOF_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR0613069.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /locus_tag="ACHUOF_004140"
     CDS             1348..1608
                     /locus_tag="ACHUOF_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR0613070.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="ACHUOF_004141"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="ACHUOF_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR0613071.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="ACHUOF_004142"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="ACHUOF_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR0613072.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          915 a          812 c          834 g          788 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcg
//
LOCUS       DAZOYF010000273         3348 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZOYF010000273 DAZOYF010000000
VERSION     DAZOYF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.279"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            19..897
                     /locus_tag="ACHUOF_004143"
     CDS             19..897
                     /locus_tag="ACHUOF_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0613073.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="ACHUOF_004144"
     CDS             894..2285
                     /locus_tag="ACHUOF_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0613074.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="ACHUOF_004145"
     CDS             2282..2539
                     /locus_tag="ACHUOF_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613075.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="ACHUOF_004146"
     CDS             2634..2834
                     /locus_tag="ACHUOF_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFR0613076.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="ACHUOF_004147"
     CDS             2827..3207
                     /locus_tag="ACHUOF_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR0613077.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          726 c          969 g          768 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaac
//
LOCUS       DAZOYF010000274         3325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZOYF010000274 DAZOYF010000000
VERSION     DAZOYF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.280"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..208
                     /locus_tag="ACHUOF_004148"
     CDS             <1..208
                     /locus_tag="ACHUOF_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613078.1"
                     /translation="RHPDKRFIFNGNEIWKRGESTTEYTRAHQRTIRRLRRLIARKQY
                     TEKWFRKYDTWSAGYYELMATKPF"
     gene            269..457
                     /locus_tag="ACHUOF_004149"
     CDS             269..457
                     /locus_tag="ACHUOF_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613079.1"
                     /translation="MNIYFRIVISLAIIACIYGLIVPALISMKDTVAVISGFALACLT
                     PPCIYAIYKGLSFSKDKR"
     gene            454..1281
                     /locus_tag="ACHUOF_004150"
     CDS             454..1281
                     /locus_tag="ACHUOF_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR0613080.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="ACHUOF_004151"
     CDS             1325..2074
                     /locus_tag="ACHUOF_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR0613081.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="ACHUOF_004152"
     CDS             2071..2640
                     /locus_tag="ACHUOF_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613082.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2637..2759
                     /locus_tag="ACHUOF_004153"
     CDS             2637..2759
                     /locus_tag="ACHUOF_004153"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613083.1"
                     /translation="MKIEYQDAAGGESMSWPDAIVTLGMVFAVAFVVYSICRWG"
     gene            2764..3006
                     /locus_tag="ACHUOF_004154"
     CDS             2764..3006
                     /locus_tag="ACHUOF_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR0613084.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3010..3156
                     /locus_tag="ACHUOF_004155"
     CDS             3010..3156
                     /locus_tag="ACHUOF_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613085.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3165..>3325
                     /gene="xisR"
                     /locus_tag="ACHUOF_004156"
     CDS             3165..>3325
                     /gene="xisR"
                     /locus_tag="ACHUOF_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFR0613086.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          928 a          797 c          854 g          746 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccgggct gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagcc
//
LOCUS       DAZOYF010000275         3312 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZOYF010000275 DAZOYF010000000
VERSION     DAZOYF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.281"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..171)
                     /locus_tag="ACHUOF_004157"
     CDS             complement(<1..171)
                     /locus_tag="ACHUOF_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HFR0613087.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATFAVMTPTY"
     gene            complement(277..1293)
                     /gene="phnD"
                     /locus_tag="ACHUOF_004158"
     CDS             complement(277..1293)
                     /gene="phnD"
                     /locus_tag="ACHUOF_004158"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0613088.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(1318..2106)
                     /gene="phnC"
                     /locus_tag="ACHUOF_004159"
     CDS             complement(1318..2106)
                     /gene="phnC"
                     /locus_tag="ACHUOF_004159"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR0613089.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(2239..2682)
                     /gene="yjdN"
                     /locus_tag="ACHUOF_004160"
     CDS             complement(2239..2682)
                     /gene="yjdN"
                     /locus_tag="ACHUOF_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFR0613090.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(2840..3175)
                     /locus_tag="ACHUOF_004161"
     CDS             complement(2840..3175)
                     /locus_tag="ACHUOF_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR0613091.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT          693 a          851 c          933 g          835 t
ORIGIN      
        1 gtaagttgga gtcattaccg cgaaggttgc catgttgccg ccgtctttaa tcagcgtaag
       61 cggggccatt tccgcgccct gccacgagac gaccagcacg tcgagaacaa cggcccagct
      121 cagaagcgag aaccagctac gcttgggtgg ggcgatggtg atggtttgca tgtttggctc
      181 cggtctgtag gccggataag gtgcttgcac cgcatccggc atcaacgcct acacattgtc
      241 ggatgcgacg ccggggcgtc ttatccgacc tacgatttac tgcaccgctt tactcaccga
      301 actcatcgcg cttaacgcgt tgttcaggcg gtccaggtca tccagctgcg cctgaatctc
      361 ggtggttttc gccagcttgt cctgctcatt cagcccttta ttgcctttca cgctttgtat
      421 ctctttaaac agtgcgagct ggcgaatcgg caccagttgc aggtcgctgg aagcgcggaa
      481 cggcgcccag cccaggcgtt ccagcaccgc tttctcttcc ggcgtttttc cgtagttcat
      541 aaagaagtcg tagatcttgt ctttggtagt ttcggacaga ttcttgcgcc agacgatcgg
      601 atcgcctagg atcagcggcg atttccagat caccttcagc tctttaagct tctccggcgc
      661 ggaggttttc agcttgtcga ggttttcggt gttgttggtg gcaacatcca cttgcttgtt
      721 ggcgacggcc agcgcgttgg tttcatgccc ggcgttgacg gtgcgcttga agtcgctggc
      781 ggagatattg tttttagcga agacgtagta accggggacg aggaagccag aggtggagtt
      841 aggatcgcca ttgccgaagg tgagatcttt ccgcttcgcc agcagatcgt tcaggttgtt
      901 gatcgggctg tctttgttga cgatcaacac gctccagtaa cccggcgatc catccgccgc
      961 gaccgtctgg gcgaagacct ggccattggc gcgatccacc gcttccatcg ccgacaggtt
     1021 gccgtaccag gcaatatcca ctttattgaa gcgcatcccc tggataatgc ccgcgtagtc
     1081 cggggcaaag aaggcgttga ccttcacgcc cagtttcttc tccatatcct gtaaaaacgg
     1141 cgtccattgc ggtttcaggt tttgctgtga ttccgttgaa ataatgccga aattcagcgc
     1201 cttttcctgc tcttcggcgt gtgccggggt taacagggtg ctgaggctga acatgctggt
     1261 gaaggccagc gaggcaatta tcttagcgtt cattcgtttt cctcaatgat ggggatatca
     1321 ggcagctttc gcgttctctt cgatgcggtt aatgctgcgg tagagatggt caaaacgttc
     1381 gttatcaaac agttggctgc tgccgtcgta gaagacgtgc ccctgacgca gggcgacgat
     1441 gcgttcgcag tagcgcaggg cgtaatccac ctgatgcagc gtgacgacca cggtgatgcc
     1501 gtcgttctgg ttgatgtcgc gcagggtgtc catcacgatg cgcgctgatt ccgggtccag
     1561 cgaggcgatg ggttcatcgg ccagaatcac tttcgcctgc tgcatcagcg cacgggcaat
     1621 cgccacacgt tgctgctggc cgccggagag ggtggaaacg cgctgatggg caaaatgcac
     1681 catgccaacg cgggtcagcg cctgtaacgc gcgctgtttc tgctcgccgg tgaaccagct
     1741 aaaacaggtg cgccagaacg gcgtgctgcc gagcgcgcca atcagcacgt tctccagtac
     1801 gctcaggcgg ttcaccaggt tgaattgttg aaagatgtag ccggtgttgg cgcggctttt
     1861 gcggatatcg cgggccaggc ggccttcgcg ctggacagtg cggcccagta gctcgatatg
     1921 gctaccggcg gatttatcgc cggtaatcaa accgcttaaa tgacgtaaaa gggtggattt
     1981 gccggaaccc gacggcccaa gcagagccac catttcactg tgatgaatgt tcagatcaac
     2041 cgcatgcagc gcttgatgct gattgaaggt tttggcgagc ttctcgacac ggataatcgt
     2101 ttgcatgatg cagcctccct aaaaaagtgg ctccatcgtg gcggattatt gtgacgattt
     2161 ggttaattaa aagtgactaa cagatgaaga gttaacggga attcgatgac agtgcgggag
     2221 ggcgggcctc ccggcgggtt attgcgttgg ttgttgtttg acgacattaa tcatccacgg
     2281 tacgccaaat ttatcggtga ctttgccaaa gccatgcgcc cagaaagttt cctgccaggc
     2341 catttcgatt tttccgttag cggcaaggtt atcaaaccag cgttttcctt cttcgacctg
     2401 ttgcgaatcg agcaccagcg taaagccgga gtagctggct tttcctgacg gtatagcatc
     2461 gctcatcatg atgtcgcttc cggcaatgcg cacgttggca tgagcgatgg cggtatcggg
     2521 aaattgcatt ccggaagggc agttctcggc gctgtcctgc gctgatttcg gcatttcgcc
     2581 gaagctgatt ttatagagca gttccgcgcc caacgtacgt tgataatagg caatcgcgtc
     2641 ggaacagtta ccggcaaaag agaggtaggg acttaacggc atgatggtga cctcagttaa
     2701 gagaagccag ttaagtgtag ttcgaaattt atacagatga gaggctgcct gataagacgc
     2761 gcaagcgtcg catcaggcag tcggcatcgt tgccggatgc ggcgcgagcg ccttatccgg
     2821 cttaccgatc acaatacaat cagttctttt tcacaaactc agatttcagt ttcatcggac
     2881 caaaaccgtc gattttgcaa tcgatgttat ggtcgccttc aaccaggcgg atgtttttca
     2941 ctttggtgcc aattttcagc atcgaagagc tacctttcac cttcagatct ttaatgatgg
     3001 taacgctgtc gccgtcagcc agcagattgc cgttagcatc tttaacgatc agctcgtcgc
     3061 tttcctgtgc aggttctgcg tcgttccatt cgtaggcaca ttccgggcag atgtacatgc
     3121 cgttatcttc gtaagtgtat tcggagttgc attttgggca gtgtggtaat gacatgtgga
     3181 tttcctcaaa agtcagcagg gcaaaagcgc caaaaaacgg cagattgccg aaaaaggccg
     3241 caattataca caaaatccct gagtttgtcg ggggtactgc tgattttaga cgacgatcgt
     3301 tttcatttga aa
//
LOCUS       DAZOYF010000276         3310 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZOYF010000276 DAZOYF010000000
VERSION     DAZOYF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.282"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            110..445
                     /locus_tag="ACHUOF_004162"
     CDS             110..445
                     /locus_tag="ACHUOF_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR0613092.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(726..834)
                     /locus_tag="ACHUOF_004163"
                     /pseudo
     CDS             complement(726..834)
                     /locus_tag="ACHUOF_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(1357..1433)
                     /locus_tag="ACHUOF_004164"
     tRNA            complement(1357..1433)
                     /locus_tag="ACHUOF_004164"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1397..1399),aa:Arg,seq:tct)
     gene            complement(1535..1610)
                     /locus_tag="ACHUOF_004165"
     tRNA            complement(1535..1610)
                     /locus_tag="ACHUOF_004165"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1575..1577),aa:Ile,seq:cat)
     gene            complement(1755..2576)
                     /locus_tag="ACHUOF_004166"
     CDS             complement(1755..2576)
                     /locus_tag="ACHUOF_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0613093.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(2591..2947)
                     /locus_tag="ACHUOF_004167"
     CDS             complement(2591..2947)
                     /locus_tag="ACHUOF_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0613094.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(2960..>3310)
                     /locus_tag="ACHUOF_004168"
     CDS             complement(2960..>3310)
                     /locus_tag="ACHUOF_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR0613095.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT          934 a          890 c          639 g          847 t
ORIGIN      
        1 aataaacaat aaacaacatc agaaaaataa ttctgttcta tggtttaatt caaaaatatc
       61 atttatactt ttcagaacat caccagcaag gcataaacaa ggaaactaaa tgaagtggat
      121 tgtgattgat acagttatcc agccatcatg cggaatatct ttttcagtca tatggagtaa
      181 aataaaatta ataatctggt atcaatcgga tgctttctta cctcctgaaa gtatatttac
      241 actgactcac acaggcatca tgctcaataa caaagtgcta cctgtaacca tttacaacgt
      301 agtaccattc aataaaacat tctggaattt aatcaaaaac agccaggaat gccctacaaa
      361 tacagataac gtattgaatg aatgctttaa taaccgttgc actctgcaaa tatgccctta
      421 tggactaaaa caacaaagtc cataaggagt ttactcacat ctgacaaaat caatataaac
      481 agcccctccg gagaggggct ggagagtggc gctatgtgcc attccatggt gccgggtgcc
      541 tcccggtgaa ttcagtacca gcacctgaat ccgcgattat cccatatacc tactcgctga
      601 ttgcccctcc gcacaggggg attcaccatg ccagtttctt ttaacaaact ccccgcaaac
      661 cagacaacag tcaaccgcct gaattgtgaa gtatttaaaa atttctcccg ctaactgata
      721 cccggctaac agtctggcgt tttctttttc agcaacggga aagcaacaac caccacaccc
      781 gccaccagca caccgtcagc cggcactgac attatccggc tgctgcaatg ccattaacaa
      841 aaacagtaag caatcacttt ttaccgtaac aggtgataat ccagatatgt atctacccca
      901 gatgagtaat ccgaagttca tccataccac aggtcctggc tattctgttg tactcctgaa
      961 caagagcaaa taattctgaa ttagcaacca tgaactcatc gcaaaccctc tgtatagcat
     1021 cactattcag aataataacg tctcttcccg aaagacgatc aggagtacag aacaaaactg
     1081 tcaaacggct gaaggccttt gctcgtgctg cattgactat atcaatacgc tgcctaagga
     1141 tgaaacaccc cgacgcctca tcaatattca ctctacccac accatatgaa tgataaatac
     1201 ttaatgctga aaaaaccatt agaccgtata acaaatactc aatcaatact taacagaact
     1261 tttatttttg acaaatatat aatattttca acaatatcct gagccaggta tatttcagta
     1321 taaggctctg ccggaaggaa tctggaagaa tgaatatggc gcgctgtact ggattcgaac
     1381 cagtgaccga ttgcttagaa ggcaattgct ctgtccggct gagctaacaa cgctgaatac
     1441 cgataatgga ccgccatcgg ggacccgccc cccgcaccaa caaccctgtt atcgtgtcgt
     1501 ctgctcttcc tgataagcta atggcggttt gtgatggtgg cccttgctgg atttgaacca
     1561 gcgacctggc gattatgagt cgctcgctct caccactgag ctaaagggcc gggagcagaa
     1621 taataacggt ccgtaattaa tttcgcaata aaaaaacccg ctcagcggcg ggttgtagaa
     1681 actcttctaa cgccaggcat aaaacgccca tcattatgac gaatttacca cagattccgg
     1741 aaaaatcaac cttgttacct agttaccttt tttaactgcc gctcagccca tgcttcttca
     1801 atatcaaacc gggtcaccag cgcatcataa aatttcttaa ctgttttttc ccatgacgcg
     1861 cgtgttatct ggtttgtcac ctcgcatata gcattaaatg cctccgttga tggtagtctt
     1921 tcatagccac gaccaccaca acgctggcag tctctgataa caggcatacc acgttttacc
     1981 gactcttcac gatgaatggc gacaccacgc ccacggcaat ccttacaggc ggtggaaacc
     2041 tcaccctttc cgccacactc cggacaggca acttttacca cctccctgac ttttttccat
     2101 tcttcccagt aaaacggata cacacctttc gtacactttg cccataccgg cggcttacca
     2161 tccggatact ggatcttgtt tgtaaaaact atgctttcaa taaatttttc cccatagcaa
     2221 caagggcact gctttttact cgctgcgctg cgggcataat cctcaaaagc gtacgaagcc
     2281 ataatgcgca tcactaccgg ttttatttct gccggaagtt ttctcaacgc cgccacacga
     2341 tcgcaccgac tgagtgcata atctgccagt aattctgttg cccgcgccct gtcattcata
     2401 ctgatgccca ttttccccag gaacgcagaa aaacccatct cagcccgatt ctgtgtcatg
     2461 ccctgcgcgg ccatcacatc agtgatactc agcgcatctt ttgacgttga ggccgatgca
     2521 tcggtcaggc caggggattt tggggagtag tatttcggta aatcttccag tttcattttt
     2581 tgacctgctc ttaatgcatt atggggtaaa tcttcacccc cagacgtcca ccagatactg
     2641 gctgaccacg aacgatattg atttcatcaa actgctcatc gtccattaac actcccgcat
     2701 gcgtcagcgc atccagcggt gctttcagga tattgtccag gtcgcgacga cgcttatccg
     2761 gtggctctgc aatcaccttt atcgccagcc ttccggacag gcttaatttc agccgctgct
     2821 ggcgaacaat aagcgccaca gcccggcgat aacgctttcc ctcctccgag ataaaatatg
     2881 tgctgccacg gcgtcgccag taagtgttca ccgtcggcgg gtaaggtaaa accaaatcta
     2941 tgagcatcag tcacctcttt tacccaagca cgccagttgc aaaggcgtga tcaagaaaac
     3001 gaaaaattaa atcaacctga gaaccatgct tttcttcgaa cgccagagga tccgcatgaa
     3061 gctcgttgtg atgctcccga cacagcggta gcgtgaaaat atcgtgagat ttggtcccca
     3121 ttccgccctg accatgacca atcaggtgat ggggatcgtc ggctggctta ccacaacacg
     3181 cacacggctg tgtcttcacc cagcgtttgt atttctcgtt aacccagcgg cgacgtttag
     3241 gtcgtttcat gaaagattcc ggagactcag gatcaacggc aatgctgacc accgtctttt
     3301 cctgttgtgg
//
LOCUS       DAZOYF010000277         3276 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZOYF010000277 DAZOYF010000000
VERSION     DAZOYF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.283"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            191..1670
                     /locus_tag="ACHUOF_004169"
                     /pseudo
     CDS             191..1670
                     /locus_tag="ACHUOF_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(1695..2033)
                     /gene="maoP"
                     /locus_tag="ACHUOF_004170"
     CDS             complement(1695..2033)
                     /gene="maoP"
                     /locus_tag="ACHUOF_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR0613096.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2152..2991
                     /gene="hdfR"
                     /locus_tag="ACHUOF_004171"
     CDS             2152..2991
                     /gene="hdfR"
                     /locus_tag="ACHUOF_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR0613097.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     DVY"
     gene            complement(3087..3162)
                     /locus_tag="ACHUOF_004172"
     tRNA            complement(3087..3162)
                     /locus_tag="ACHUOF_004172"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3127..3129),aa:Trp,seq:cca)
     gene            complement(3171..3247)
                     /locus_tag="ACHUOF_004173"
     tRNA            complement(3171..3247)
                     /locus_tag="ACHUOF_004173"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3211..3213),aa:Asp,seq:gtc)
BASE COUNT          882 a          798 c          803 g          793 t
ORIGIN      
        1 ttcagtataa atgttgttcc cttcctcgta gttcatcctt tgtccgctgc atttttgcga
       61 gcatcattcc accgccagaa caactcaact tcacttgcac aaataaacag gaatcagcca
      121 ctaaaattca cgaattagcg ggctacagca gcgatatctt atgcacataa tggtgactca
      181 aggagggttt atgtcactgt caattgttca tacccgcgca gccctggaag taaatgcgcc
      241 cccaatcact gttgaggtac atatcagtaa aggtctaccc ggcttaacga tggtgggctt
      301 aacagaaaca acggtgaaag aagctcgcga tcgcgtgcgc agcgccatta tcaatagcgg
      361 atatgaatat ccggcgaaaa aaatcaccat caaccttgct ccagccgatc tgccaaaaga
      421 agggggacga tatgatttac ctatcgccat tgcgttgctg gcggcctcag aacagcttac
      481 agccaataag ttagatgaat atgaattagt cggagaacta gcgcttacag gcgctctgcg
      541 tggcgttccc ggtgcaatct ccagtgcaac tgaagctatt aagtcgggca gaaaaattat
      601 cgtcgcgaaa gataacgaag atgaagtggg gctaattaac ggtgaaggat gcctgatagc
      661 cgatcatctg caggctgtct gtgcgtttct ggaaggtaag aacactctcg aacgcccgaa
      721 accaactgat gcagtatccc gggcgctaca acatgatctc agtgatgttg tcggtcagga
      781 gcaaggaaaa cgaggactgg aaattaccgc cgctggtggg cacaaccttt tactgattgg
      841 gccgccggga acaggtaaaa caatgctcgc cagccgtatt aatggcctgt tgccagattt
      901 aagcaatgaa gaggcactgg agagcgctgc gatattaagt ctggtaaatg ctgaatcagt
      961 acaaaaacaa tggcggcagc gcccgttccg ctcacctcat cacagtgcgt cgttaactgc
     1021 gatggtgggc ggtggtgcaa ttccagggcc aggtgaaatt tcgttggcgc ataacggcgt
     1081 gctttttctt gatgagctac ctgaatttga acggcgtaca ctggatgcct tgcgagagcc
     1141 gattgaatcc gggcagatcc atctttcacg cacacgagca aaaataacct atccagcccg
     1201 tttccagctt gttgcggcga tgaatcccag ccctaccgga cattatcagg gaaaccataa
     1261 ccgctgcacg ccagaacaga cattacgtta tctcactgga gatcccatta ccaccccccg
     1321 gcattttgag taaaacggta gtgccgggag aaagcagcgc caccgttaaa caacgcgtaa
     1381 tggccgccag agagcgccaa tttaagcggc agaataaact gaatgcctgg ctggatagtc
     1441 cggaaatacg ccaattctgc aaacttgaga gcgaagatgc gatgtggttg gaagaaacgc
     1501 tgatccatct ggggttatcg attcgtgcct ggcagcggtt actgaaagtt gcacgaacca
     1561 ttgctgatat tgatcagtct gacattatca cacgtcagca tttgcaggag gcagttagct
     1621 atcgtgcgat tgaccgtttg ctcatccatc tgcaaaaact actgacataa aaaaagggca
     1681 tttcgccctt tttattaatc gtcagaatcg gtgtagtctt cagcaccttc aacctgcggt
     1741 ttaccgccgg aaagggtgtg aaaacgtttt ggacgcttaa tacgcgtcat atacttggac
     1801 cacacgcgtt ctgcttctgt cactggctca cgttcgccac ggcatactgc tacgaagagt
     1861 ttctcttcct cggtaaccgg ctcgcgtttg ccaagatcca actcattgaa ggcataacca
     1921 tgacgctcaa gcagttgtgc ctctttgatg gtgaaatcac catgacgaga gaatccacgt
     1981 ggataatgtt tattgtcgaa atatcgatta gtcgtcgtaa agctttccgc catcctgcac
     2041 gctcctaatt ctttgaccga gctagttatg gcgcggagta ttagttacgc ttgacagagt
     2101 gtaaaacaaa acatttaaat cataacgaca aataattttg cggagagcac tgtggatacg
     2161 gaattgttaa aaactttcct ggaagttagc cgaacgcgtc actttggtcg agcggctgaa
     2221 tcgctctatc tgacccagtc agcagtgagc tttcgaatca gacaactgga aaatcaactg
     2281 ggtgtgaacc ttttcacccg ccacagaaac aatatccgtt taaccgctgc cggtgaaaaa
     2341 ctactgcctt atgcagaaac gctcatgagc acctggcagg ccgcccgcaa ggaggtggcg
     2401 catacctcac gacataacga gttttctatc ggtgccagcg cctcgttgtg ggaatgtatg
     2461 cttaatcagt ggttgggacg cttgtatcaa aatcaggatg cccatacagg cttacagttc
     2521 gaagcgcgaa ttgcccaacg gcagtctctg gtaaaacagc tgcatgaacg ccagcttgat
     2581 cttcttatta ccactgaagc gcccaaaatg gacgaattta gtagtcagtt gctggggtat
     2641 ttcactttag cgctttatac cagtgcccct tctaaactaa agggagatct taactatctg
     2701 cgactggagt gggggccaga ttttcaacag catgaggcag gtttgatcgg tgctgacgaa
     2761 gttcccattc tgacaaccag ttctgctgaa ctggcacagc aacagattgc gatgcttaat
     2821 ggttgcacct ggctacccgt cagctgggcg cgtaaaaaag gcggcctgca taccgttgtc
     2881 gatagcacaa cactttcacg gccgctttat gccatatggc tgcaaaatag cgataaaaat
     2941 gcgttgattc gcgatctttt gaaaattaac gtgctggatg atgtgtatta atctgaatgg
     3001 ctggcaagga tgccggtaga aggatttact tcggagagga ttatttcaga taaaaaaaat
     3061 ccttagcttt cgctaaggat gatttctggc aggggcggag agactcgaac tcccaacacc
     3121 cggttttgga gaccggtgct ctaccaattg aactacgccc ctaattaggg tggcggaacg
     3181 gacgggactc gaacccgcga ccccctgcgt gacaggcagg tattctaacc gactgaacta
     3241 ccgctccacc gaattctttt acaaccaccg gtttta
//
LOCUS       DAZOYF010000278         3252 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZOYF010000278 DAZOYF010000000
VERSION     DAZOYF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.284"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            274..549
                     /locus_tag="ACHUOF_004174"
     CDS             274..549
                     /locus_tag="ACHUOF_004174"
                     /inference="COORDINATES: protein motif:HMM:NF018642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFR0613098.1"
                     /translation="MRDVQWFCPLTPVHTGTEIKPVEMPVTTITPVSDAGGLQDFIYW
                     RPDADRAGMEPVYVILNDPLDSGRFTRKQLNKNILNMQVILVFSIQE"
     gene            747..2111
                     /locus_tag="ACHUOF_004175"
     CDS             747..2111
                     /locus_tag="ACHUOF_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0613099.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
     gene            2161..2370
                     /locus_tag="ACHUOF_004176"
                     /pseudo
     CDS             2161..2370
                     /locus_tag="ACHUOF_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(2464..2655)
                     /locus_tag="ACHUOF_004177"
                     /pseudo
     CDS             complement(2464..2655)
                     /locus_tag="ACHUOF_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2696..3193)
                     /locus_tag="ACHUOF_004178"
                     /pseudo
     CDS             complement(2696..3193)
                     /locus_tag="ACHUOF_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          800 a          719 c          768 g          965 t
ORIGIN      
        1 gggttgggga gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat
       61 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
      121 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      181 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      241 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      301 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      361 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      421 agggctggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      481 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      541 caagaataaa tagtagtaac tactatagct gcaaataatg ttaaaaacat ttctatgatc
      601 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      661 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      721 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      781 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      841 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      901 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      961 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
     1021 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1081 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1141 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1201 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1261 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1321 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1381 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1441 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1501 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1561 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1621 aggatatggg aaaactggca tcaatggggg ggggttctgt ttttgaaatc ggtggtgtgg
     1681 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1741 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1801 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1861 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1921 gagccgcatc agggctgtca ggcacatttt cctatatctt cggctcaatt gttgcgacac
     1981 tgggaatggg acttgttgtc gattatctgg gatggggggc cacatttatc gtactgatac
     2041 tgtctgctgt ttttgccatc attttcacac tgatgagtcg tgaacgctca ctggagttcg
     2101 aaaaagagta attttctcag gaaaaacata aaattactga ctaaatattt attttttgaa
     2161 tcgcagggca ttccggataa ggttgatatc gtgtcacggc tgaagaccta tggatacagt
     2221 atttctggtg tggagacgga tgacggatat aaggcgcttg tccgcgcttt tcagcttcat
     2281 ttccggcaga aaaattatga cggcataatg gatgctgaaa ccgctgccat cctgtatgca
     2341 ttgctggaga agtatttccc gggtaaataa ggagtgagtc gtcagagctg cgatatatct
     2401 ccttcctcca tgggctgtct cttatacaca aatccccacc cggggatttg tgatgtcaga
     2461 tgtccccagt aagactatgt tttcattgcg ctctatgaag ctgagtgagc gtaacgactg
     2521 gagctgcttc tgcggtgctc cggtggcgaa cgtgaagtca tactcttcga acattttcac
     2581 cgccgggaag gctgccattc gggtatacat cgcctgttta cgttgatgac gtgccagttt
     2641 ttcttcatga agcatgatgc tccaagtaaa cgttcaccga acaccgtatt gacgattatc
     2701 ctgatggtag caccaccttc catggcagga ggtcgtcaac tcggttggaa ggccattccg
     2761 gcagtacgct cagaatatgg cgcagatacg cttccggatc gataccgttc agacggcagg
     2821 tgccgatcag cccgtacagc agtgcaccac gctcgccgcc gtgatcgcta ccgaagaaca
     2881 cgtaattttt ctttccgaga cagactgcac gaagcgctct ttccgctgtg ttattgtccg
     2941 cctccgccag accgtcatca ctgtaataac agagggcgtc ccactgattc agtacatagc
     3001 tgaatgcttc gcccagtctg gattttttcg acagcgtgcc attcttctcc accatccatt
     3061 catgcagcga cgtcagtaac gctttgcttc gctgctgcct gactgcaaga cactctgact
     3121 ctggtaatcc ccgtatttca tcctcgatgg cgtacagttc actgattcgc ttcagggctt
     3181 cttctgccgt cgcacgcaag taatccgggg cccgctatgc cgcgctctat cgggcgggac
     3241 ggggctggcg ct
//
LOCUS       DAZOYF010000279         3208 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZOYF010000279 DAZOYF010000000
VERSION     DAZOYF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.285"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(26..1114)
                     /locus_tag="ACHUOF_004179"
     CDS             complement(26..1114)
                     /locus_tag="ACHUOF_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR0613100.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(1111..>3208)
                     /locus_tag="ACHUOF_004180"
     CDS             complement(1111..>3208)
                     /locus_tag="ACHUOF_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR0613101.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
BASE COUNT          818 a          823 c          697 g          870 t
ORIGIN      
        1 aaaagcgttt gtccggtttg cagggttaat tgatggttgg caaggaaggc gtaaagatta
       61 ctgacaattt gcccatgtag tacccggcct gtttatccat cacgctgaat ttatcgactg
      121 ccagagtcaa gggtgtcggc acacacatca cggcatagcg aataccgggc agtaccaccg
      181 gacgcacacg tttcaggtta atgctgttcc agaccatatt ttcattattg cgcacatcaa
      241 tttgcccacc ggtttccggg ttgtacattt tgacccggta atcaatgcgt tctccctcgt
      301 cggtggtggt ccctccctga cgatagatag agaagtaacc gtctctgcgc cctgcagctg
      361 gtcgcccttc atctttgagc atgatttcat aactgatggc attaccgtta aaaccatcgt
      421 acaggcacat gtccaacata gtcagatctt gcgcgtagtt gctgccgtta acgcttcctg
      481 ttggatgcaa gtctaactgc acccttggcg tggcgtgggc gaactccggg aagaacacgt
      541 caatatggtt ggggtcgagc gtattcaggg taatatttgc gaaatagtcc tgggccgggc
      601 tgttcgaatg taatttaact ttccccttcc agacgccacc aatcggcaaa ttcttcatct
      661 ctgtctgctc aaggtagagc gtaagaaact ttgtatcgta tatataataa tgttgaccat
      721 cttctgtaca tgaagatcta ttagaatcct taagattcgt ctgaagccct ttgcccccct
      781 tgcatcctag cggcgcttca tcgctactta caatggctgt tttaaatcct ttgagtttta
      841 ccggccaacg catcccactg cgttgttcag ttaaatagat ttgtactgtc gatatatttc
      901 cattagctgt acaggtttgt tggaggtcag gatacgaaca ttcataccac atatatccat
      961 tatttcctgc cgacgcttca tccttccaga agtaataatc cgctctacca gatgccaaat
     1021 catacgtaat ttcactggtc acgtcaggag ctgcctgaac gccagtgagc gtgccaaaca
     1081 aacccaatat cgccgcaatc aatcgatttc tcatggtgca acctcccttt gcgccgtctg
     1141 aacgtttcca cgtaatttag gctgtttcaa ctgtgcctga acctgaatcg actccggtaa
     1201 ttgtgaataa gtcaccacac tacaagtaac atctccgctg taacgaatgc tggcgcgttt
     1261 ctttacaaca ctcagtggac acttaaggaa ttgctgcccg gaaagcagat agagctcttt
     1321 ttcgctgccg ttaatttcaa gtgtaaatcc gccatcttca gctaccagta acatggcgct
     1381 gttcagcccc acaatcggga attttttcac cgctgccggc agtaataaac gaccgatata
     1441 gttgtagtta aagctggcag acacttcgcg atagcgcagc ttgccaggca acaacattaa
     1501 ttcacgattc cctgcaccgt taatgatgtt tgttgtcccc tgctgattgc cctggctact
     1561 gtcgttaacc tcaacaaaac cagactgaaa tccaggataa gggaacaaca cactgtcact
     1621 ttgaccaagc tgcaaagttc ggctgcctgc cacccgcgct tcgagttgca gatcctgact
     1681 ctcttcatcc tgttcagaga agcctttcac atccaccagt accgcaccca gcaaatcgtc
     1741 tgtaccactg gctcccaaat taacgccata gcgactgaca gccagcgtag agctataagt
     1801 gcccgtaaag gccgaaagat gatcatgatt cttacggtca taactatcag atacggtagc
     1861 gtagacattt ccgtattgcc catcccaacg cccgttaacc gcgctgttta tggtatcggt
     1921 gttcaggcct ccgacggtta cgccaagttc tttatggctg aatgaatcgt tataaaaggt
     1981 atgcgataac tgccatgacg tttgatcgcc atcctgttcg ctataacgat aatccgtagg
     2041 cacattggtt gaatggctgt tgttattgct atccatcgtt ggcgtgtcag ataacgaaaa
     2101 cgataagtac aagccgttgt cgtcgttatc gccaccgttg tcattacgat ggaaaacacc
     2161 gactgacgta ctcaaaatcc agtcacgcat cacaatagcg cgggaagcgc taagttgtac
     2221 agtttcagag atactttgcg ctgttgtctg gcgaagatag ttattttcaa agctctgctg
     2281 tttatcccaa cgcaaactat catcagtgtt attttgtgtg caggtataac ccagcatcat
     2341 attccagccg acaaaattca gtgaaactgt cgcggaaata ctttcataac agcttaaggc
     2401 gttatagctc tctctgctgt cggttgtaca agcatcgccg tcagagttgg tccgataaaa
     2461 gcccaacgtc ggccatcccg gattactcca gttggcctgt tgcatatcac ctttgccgcc
     2521 gtcatcgtta cggaacacgc tggcgctgat tgcaagattc cccaccctgc ctaaatctgc
     2581 cgtccagtta ttacctaact cgaaagcact cacatcatca gcattcgcca gccctgcgta
     2641 gagctcatat tgcggatgta atggcaggcg tactcctagc tgataagctg aactttcatc
     2701 atcagaaacc tgtgatgtag ttttacctgc ctgtaagaac cattgcgcat ttccgtcagt
     2761 cagaccgccg gttttggtaa acggcacaag ctcggtgcgg gtgagttggt tattttcgta
     2821 gacttttaat gccacgctgt agctgcctgg cggaaaggaa ctggtatcaa taaattgcga
     2881 accactattg agataaaacg atcccaaaag ttgctcatta cgataagcgt caacacgaga
     2941 attgcgcgaa agcagaacca ttactggggt tccttgctgg ctttgcgcct ggtttaaata
     3001 gctcagtgtc gacccgatac gcatcccatc gattgcaccg agtggcagaa agttaaaggt
     3061 aaagttcccg ccttgtgcat taaatagtgt gcggttgtcc atgcgacccg cctgcacata
     3121 ataacgacgt aaaaaatcat aacgataata gagatcgctg acgtcagtat tactgtcact
     3181 gacatcatct gcatcataac cgttgaaa
//
LOCUS       DAZOYF010000280         3180 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZOYF010000280 DAZOYF010000000
VERSION     DAZOYF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.286"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            419..1528
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUOF_004181"
     CDS             419..1528
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUOF_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR0613102.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(1773..1907)
                     /locus_tag="ACHUOF_004182"
     CDS             complement(1773..1907)
                     /locus_tag="ACHUOF_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0613103.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2553..2723)
                     /locus_tag="ACHUOF_004183"
     CDS             complement(2553..2723)
                     /locus_tag="ACHUOF_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR0613104.1"
                     /translation="MNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSI
                     QIRFEAESETGT"
BASE COUNT          801 a          659 c          775 g          945 t
ORIGIN      
        1 aaaaaaaaac attatcttat attaggggtg ctgctagcgg cgcggtgtgt ttttttacag
       61 gacgcccctg ggggcgctgc taggggtgtc tgttcagata tgtagcaatg agtcagggat
      121 tcacgaactg aaaatgtccg aaaatgtgtc tcatgggcac tgtatgcgcc caaaccgatc
      181 gtcattcgct ttcatgcata gctatgcagt gagctgaaag cgatcctgac gcatttttcc
      241 ggtttacccc ggggaaaaca tctctttttg cggtgtttgc ggcagaatcg cgttcagcgc
      301 gttttagcgg tgcgcgcaat aaaatgttat gttaaatgtc tgctgaatgg tttttaggtt
      361 ttttcctaac atatgataga ataaaaaaac catatgttaa tatattgtgt ttttttcaat
      421 gaaaatctca aaggtctctg acgaacattt ttctaattac aacatgttgc cgaatggtaa
      481 tgaagaaatt gacctttttg gcttgattag cgttttatgg gcagcaaaaa taaaaattgt
      541 tggggtgatt ctattttttg cttgtattgg ctttcttgtt tcttttgttt tgccacaaaa
      601 atggactagt tctgctgtgg ttactcctgc tgagtctgtt caatggagtg aattaaataa
      661 ggagctttcc aagcttcatg ttctgggagt ggattttgat atcaatcgtg atagcgcatt
      721 tgctctgttt attaagaaat ttcagtccgt taattcgttg aatgagtata tgagatcatc
      781 tccttatgtt atggagatta ttaaaaagaa taacattagc gctctcgaac tgcatcgtgc
      841 aattgttgag ttgagtaaaa atatgaagtc gattgatgat aatgccagta agaaaaacga
      901 caagtcatca ttgtatgtat catggactct gagttttact gctccaacaa gtaaagaagc
      961 tcacgatgtg ttgtctgggt atattaatta tgtttcttcc cttgttgtaa gggatttgat
     1021 ggaagatata agaaataaac tagaagttaa aactaatgtt gaaaaagaaa ttcttgcact
     1081 ggatgagata aaaattagaa accagctgaa tgcagatatt cgacgcctca attattcact
     1141 ggaggttgct aatgcggctg gaataaaaaa acctgtatac agcaatggtc agattatgaa
     1201 ggatgaccca gattttcctg tggctctcgg ttctgatggt atagcaacta aattgaacat
     1261 caaaaaatca atcaaggatg tttcggaatt gagtggggag ttgcgaaatc gtcaatatgt
     1321 tgtgaatcaa ttggttgtgg cgaaagtggg ggatgttgat tttatgccat ttcagtatca
     1381 attgagccca acgttaccgg tcagaaagga tgggccaagt aatgccataa ttgtaattct
     1441 ttcttctgtc attggaggga tgcttgcatg tggctatata cttttacgtc atgccatgga
     1501 ggccagaaag atgagtgtga ttgagtaaaa gttgtgctga tgcatcattt tataagcaac
     1561 ttttataaaa agttcaaagg atcttcttga gatcattccc ttacagccgt agtcttctac
     1621 tctagaaaac gaaaaaacca cctggggagg tggtttttcg aaggttcagt aagttgggga
     1681 acttctgaac cgtggtaaca gtcttgtgcg agacatgtca ccaaatctgt ccttctagtg
     1741 tagcctcagt aaggctgcca cttcaagaac tctcaataca tctctcgcac atcctgttta
     1801 ccagtggccg ctgccaatgg cgttaagtcg tgtcttaccg ggttggactc aagacgatag
     1861 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1921 gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacgaac gcggccataa
     1981 cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccacc
     2041 agggggaaac gcctggtatc tttaagtcct gtcgggtttc gccacctctg acttgagcgt
     2101 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccac cggcgcggcc
     2161 ttttttcggg gcctcttttt tgctcacatg ttttttctgg tgttatcccc tgattctgtg
     2221 gataaccgta tatccgtctt tgcgtgagca gacaccgctc gccgcagccg aacgaccgag
     2281 cgcagcgagt cagtgagcga ggaagcggaa gagcgccgga agctgcattt tctccttacg
     2341 tatctgtgca gcatttcaca cctcatatgt cgctgttctc tcaccatctg ctctgccgcc
     2401 tcataattaa gccagtatac actccgttat cgctacgtga ctgggtcatg gctgcgcccc
     2461 gacacccgct aaaaccccct gacgcgccct gacgggcttg tctgctgacg aaaacgccca
     2521 cgggaaagcc gtgaccgcct ccgtcagctt cgttatgttc ctgtttcact ttcggcctca
     2581 aagcgaatct ggatgctgtt ctggagttct tctgcgagtt cgtgcagtcg tttgcacatg
     2641 gccgcctgtt cgtcggcatc cagagcatcc agtttttcaa gcagtgtcag gctctggctt
     2701 ttgatacatc gcgccatatt cagcgcagtt tgttgttatt tgttcacctt ccgttgttct
     2761 ccgtcagttc cgggtgtcgg gggggggacc ctgagcaggt ggtaattgta agacgttgcg
     2821 ggcgacggta ttacaattgc acatcctgtc ctgtttcttt aggacgtatc atggcacagc
     2881 atatcaactg ataaataatc cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg
     2941 cgtcgagtgt ttcttaatca ataattccgc tgatactccg gaaaaacgcg agtctgttct
     3001 catgcctgaa atgcccacac cccacgcaaa aacaagtttt tgctgatttt tctttataaa
     3061 tagagagtta tgacaaatta gttcttcttg ctctctttgt gatatttaaa aaagcggtgt
     3121 cggcgcggtg ttgtagctgc gccaacaccg ctttttaggg gtggtactga ctattttcat
//
LOCUS       DAZOYF010000281         3165 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZOYF010000281 DAZOYF010000000
VERSION     DAZOYF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.287"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..1224
                     /locus_tag="ACHUOF_004184"
     CDS             <1..1224
                     /locus_tag="ACHUOF_004184"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR0613105.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1958
                     /locus_tag="ACHUOF_004185"
     CDS             1224..1958
                     /locus_tag="ACHUOF_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0613106.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2425..>3165
                     /locus_tag="ACHUOF_004186"
     CDS             2425..>3165
                     /locus_tag="ACHUOF_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0613107.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLF"
BASE COUNT          858 a          618 c          780 g          909 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgcttg caaatatagg tgatttttta
     2221 ttgtaggctt ttgaaggcta ttataactgt gcgctaaatt aatgcgttga ataaaagaaa
     2281 taacaaaatt aagaaaagcc atatttacga tatgggcaac ataaatataa tctaaaaaag
     2341 agtaaggcgt tgtaggttgg ttccgcgttc attaccttgt tagtgaacgc ggatttatca
     2401 gttgtcgaaa tggtttttat aataatgaaa aattttaatg tactaaccaa agtcgtattt
     2461 tgcttgtcgt ttttatcaag cgcatttccc acctatgctg cggatgatgt caaagtgact
     2521 caaaacactt cagtttttaa actgaagctt agtgcaacgc gattaatcta tgaacaaggg
     2581 tcggatggcg cagcgataac tgtgacaaat ccgcaggatt ggccgatgct tgtgcagagt
     2641 aaagtctttg aagaagataa agtaactgca gcgcccttta ttgtcactcc gccactgttc
     2701 aggcttaatc caggccagca aaataaaatg tgggttgtgc agactgcgcc agctttacaa
     2761 gacagggaaa aattacactg gctatgcgcc accggcatta cgcctaaaaa gagtgatgtt
     2821 tgggccgaga aagataaaga aaaaattgct gaaaatgtga acctgaacat ccaggttaac
     2881 gtacatacct gcataaaact tattaccagg ccatctggta ttgaaggcaa gatggttaat
     2941 gcgagtgaga agttatcgtg gaaaaagcag ggtgataaaa ttgtcgcggt caatcagtcg
     3001 ccgttctaca tcaacctttc ttctttacag gttggcggta gcaagattga gaaactagat
     3061 tatattgcgc cgttttcgca acatgagttt gttatgccaa aaactagcgg ccaggatatt
     3121 aaatggtctg tgattactga tgaaggtggt gagagtcgtt tgttt
//
LOCUS       DAZOYF010000282         3112 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZOYF010000282 DAZOYF010000000
VERSION     DAZOYF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.288"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUOF_004187"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUOF_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR0613108.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="ACHUOF_004188"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="ACHUOF_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="ACHUOF_004189"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="ACHUOF_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFR0613109.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="ACHUOF_004190"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="ACHUOF_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFR0613110.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="ACHUOF_004191"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="ACHUOF_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAZOYF010000283         3069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZOYF010000283 DAZOYF010000000
VERSION     DAZOYF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.289"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..498
                     /locus_tag="ACHUOF_004192"
     CDS             <1..498
                     /locus_tag="ACHUOF_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0613111.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="ACHUOF_004193"
     CDS             578..1273
                     /locus_tag="ACHUOF_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0613112.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="ACHUOF_004194"
     CDS             1302..>3069
                     /locus_tag="ACHUOF_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0613113.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAZOYF010000284         3020 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZOYF010000284 DAZOYF010000000
VERSION     DAZOYF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.290"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHUOF_004195"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHUOF_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR0613114.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="ACHUOF_004196"
     tRNA            complement(621..695)
                     /locus_tag="ACHUOF_004196"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="ACHUOF_004197"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="ACHUOF_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHUOF_004198"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHUOF_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR0613115.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          745 a          719 c          712 g          844 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa
//
LOCUS       DAZOYF010000285         3000 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZOYF010000285 DAZOYF010000000
VERSION     DAZOYF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.291"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHUOF_004199"
     CDS             complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHUOF_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR0613116.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGH"
     gene            348..641
                     /locus_tag="ACHUOF_004200"
     CDS             348..641
                     /locus_tag="ACHUOF_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR0613117.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="ACHUOF_004201"
     CDS             713..1372
                     /locus_tag="ACHUOF_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR0613118.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="ACHUOF_004202"
     CDS             1464..1910
                     /locus_tag="ACHUOF_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR0613119.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3000)
                     /gene="hlyE"
                     /locus_tag="ACHUOF_004203"
                     /pseudo
     CDS             complement(2117..3000)
                     /gene="hlyE"
                     /locus_tag="ACHUOF_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT          782 a          647 c          718 g          853 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
//
LOCUS       DAZOYF010000286         2998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZOYF010000286 DAZOYF010000000
VERSION     DAZOYF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.292"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..2850
                     /locus_tag="ACHUOF_004204"
     rRNA            <1..2850
                     /locus_tag="ACHUOF_004204"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          793 a          661 c          932 g          612 t
ORIGIN      
        1 gacgtgctaa tctgcgataa gcgtcggtaa ggtgatatga accgttataa ccggcgattt
       61 ccgaatgggg aaacccagtg tgattcgtca cactatcatt aactgaatcc ataggttaat
      121 gaggcgaacc gggggaactg aaacatctaa gtaccccgag gaaaagaaat caaccgagat
      181 tcccccagta gcggcgagcg aacggggagc agcccagagc ctgaatcagt gtgtgtgtta
      241 gtggaagcgt ctggaaaggc gtgcgataca gggtgacagc cccgtacaca aaaatgcaca
      301 tactgtgagc tcgatgagta gggcgggaca cgtggtatcc tgtctgaata tggggggacc
      361 atcctccaag gctaaatact cctgactgac cgatagtgaa ccagtaccgt gagggaaagg
      421 cgaaaagaac cccggcgagg ggagtgaaaa agaacctgaa accgtgtacg tacaagcagt
      481 gggagcacgc ttaggcgtgt gactgcgtac cttttgtata atgggtcagc gacttatatt
      541 ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa ctgggcgtta
      601 agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg aaggttgggt
      661 aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga cttgtggctg
      721 ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct atttaggtag
      781 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
      841 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      901 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      961 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
     1021 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
     1081 cggggctaaa ccatgcaccg aagctgcggc agcgacgctt atgcgttgtt gggtagggga
     1141 gcgttctgta agcctgtgaa ggtgtgctgt gaggtatgct ggaggtatca gaagtgcgaa
     1201 tgctgacata agtaacgata aagcgggtga aaagcccgct cgccggaaga ccaagggttc
     1261 ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa ggcgaggccg aaaggcgtag
     1321 tcgatgggaa acaggttaat attcctgtac ttggtgttac tgcgaagggg ggacggagaa
     1381 ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt gtaggctggt tttccaggca
     1441 aatccggaaa atcaaggccg aggcgtgatg acgaggcact acggtgctga agcaacaaat
     1501 gccctgcttc caggaaaagc ctctaagcat caggtaacat caaatcgtac cccaaaccga
     1561 cacaggtggt caggtagaga ataccaaggc gcttgagaga actcgggtga aggaactagg
     1621 caaaatggtg ccgtaacttc gggagaaggc acgctgatac gtaggtgaag cgacttgctc
     1681 gtggagctga aatcagtcga agataccagc tggctgcaac tgtttattaa aaacacagca
     1741 ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct gcccggtgcc ggaaggttaa
     1801 ttgatggggt tagcgcaagc gaagctcttg atcgaagccc cggtaaacgg cggccgtaac
     1861 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacctg cacgaatggc
     1921 gtaatgatgg ccaggctgtc tccacccgag actcagtgaa attgaactcg ctgtgaagat
     1981 gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct ttactatagc ttgacactga
     2041 acattgagcc ttgatgtgta ggataggtgg gaggctttga agtgtggacg ccagtctgca
     2101 tggagccgac cttgaaatac caccctttaa tgtttgatgt tctaacgtgg acccgtgatc
     2161 cgggttgcgg acagtgtctg gtgggtagtt tgactggggc ggtctcctcc taaagagtaa
     2221 cggaggagca cgaaggttgg ctaatcctgg tcggacatca ggaggttagt gcaatggcat
     2281 aagccagctt gactgcgagc gtgacggcgc gagcaggtgc gaaagcaggt catagtgatc
     2341 cggtggttct gaatggaagg gccatcgctc aacggataaa aggtactccg gggataacag
     2401 gctgataccg cccaagagtt catatcgacg gcggtgtttg gcacctcgat gtcggctcat
     2461 cacatcctgg ggctgaagta ggtcccaagg gtatggctgt tcgccattta aagtggtacg
     2521 cgagctgggt ttagaacgtc gtgagacagt tcggtcccta tctgccgtgg gcgctggaga
     2581 actgaggggg gctgctccta gtacgagagg accggagtgg acgcatcact ggtgttcggg
     2641 ttgtcatgcc aatggcactg cccggtagct aaatgcggaa gagataagtg ctgaaagcat
     2701 ctaagcacga aacttgcccc gagatgagtt ctccctgacc ctttaagggt cctgaaggaa
     2761 cgttgaagac gacgacgttg ataggccggg tgtgtaagcg cagcgatgcg ttgagctaac
     2821 cggtactaat gaaccgtgag gcttaacctt acaacgccga agatgttttg gcggattgag
     2881 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     2941 attagcctgg cggccgtagc gcggtggtcc cacctgaccc catgccgaac tcagaagt
//
LOCUS       DAZOYF010000287         2958 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZOYF010000287 DAZOYF010000000
VERSION     DAZOYF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.293"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..414
                     /gene="torD"
                     /locus_tag="ACHUOF_004205"
     CDS             <1..414
                     /gene="torD"
                     /locus_tag="ACHUOF_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR0613120.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHUOF_004206"
     CDS             complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHUOF_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR0613121.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHUOF_004207"
     CDS             complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHUOF_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR0613122.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1993..>2958
                     /locus_tag="ACHUOF_004208"
     CDS             1993..>2958
                     /locus_tag="ACHUOF_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613123.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKN"
BASE COUNT          811 a          631 c          705 g          811 t
ORIGIN      
        1 acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
       61 ctgatgaccg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
      121 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg
      181 gcagatcatc tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag
      241 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
      301 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg
      361 gcactaagcc agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt
      421 ttatgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat
      481 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
      541 cgtagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
      601 gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca
      661 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
      721 cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg
      781 gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat
      841 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
      901 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
      961 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca
     1021 aaatgctttc tttcagtgtt ggaacggtga ctttagtacc cagcgccgct tcccacgggc
     1081 taaccggcac cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa
     1141 tatgaatcac cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc
     1201 ctttcaggcg gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt
     1261 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
     1321 tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
     1381 ggccacgtgc ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
     1441 agatatcgtc aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgacggt
     1501 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
     1561 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
     1621 tgctgacatc aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga
     1681 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
     1741 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
     1801 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
     1861 ggtcgccatc aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt
     1921 tttgatagtt gaaccaggca ctttaagttt aactagggcg tcattattta ttaaatttta
     1981 tagacgctat atatgggtag taatatacat ggaattagtt gcactgcaaa taattatttg
     2041 aaacaggcct ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata ttcaatcacg
     2101 ctttttgaaa acacactggt gtgttttatg cggttgtaca atgaactcag acgtaaagta
     2161 aatgaagagg atactccatg tctggaatgt gaatcactag aaaaagaatt tgaggaaatg
     2221 cagaatgata atgatctatc attatttatg agaacattgc gtactaatga tacacaaatt
     2281 tattcagggg tttcaggagg tattacatat actatacaat atgttcaaga tgttgatatt
     2341 gttagagtgt ccttgccggg cagaggttca gagtctatca cagattttaa aggttattat
     2401 tggtatggct ttatggaata tattgaaaac attaatgcgt gtgatgatgt tttttctgag
     2461 tattgtttag ataatgaaaa tatgagtatc cagccagaac agataaatat gccgggaata
     2521 tctgatttag atactggtat tgatttgtct ggcatatctt ttattcagag tgaaattaat
     2581 aaaacatatg gattaaaata cgctccagta gatggcgatg gatattgtct gttaagagcc
     2641 atactggttt taaaagaaca tgagtattca tgggcgctgg gcagccataa gacgcaaaag
     2701 caagtatacg aagagtttat taaaattgtt gataaacaaa cgatcgaggc tcttgttgat
     2761 acggcattct ataatctcag ggaagatgta aagacattat ttggcgttga tctgcaatct
     2821 gacaacaaaa ttcagggtgt gggtagtttt atgtcatgga gctttctgtt ttttaaaaaa
     2881 caattcattg atagttgctt gaatgacaaa aaatgcatac tgcatttacc cgagtttata
     2941 tttaatgata acaagaat
//
LOCUS       DAZOYF010000288         2952 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZOYF010000288 DAZOYF010000000
VERSION     DAZOYF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.294"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHUOF_004209"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHUOF_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR0613124.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUOF_004210"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUOF_004210"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR0613125.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..>2952
                     /locus_tag="ACHUOF_004211"
     CDS             2604..>2952
                     /locus_tag="ACHUOF_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0613126.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          659 a          701 c          779 g          813 t
ORIGIN      
        1 aaaggggggg ctttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg gtgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgctg tcgattacgg ggggcgaggt gctgtatgct
      841 gatatggggc actttggtaa gttctctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgttgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccactgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtac tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag cgggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgccagacgc ctcctttctt cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaagct gcggatcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccaaggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg cagcgttaaa tgtctatccg ggccgtgtga
     2701 tggcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aa
//
LOCUS       DAZOYF010000289         2932 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZOYF010000289 DAZOYF010000000
VERSION     DAZOYF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.295"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="ACHUOF_004212"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="ACHUOF_004212"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR0613127.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="ACHUOF_004213"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="ACHUOF_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR0613128.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHUOF_004214"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHUOF_004214"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFR0613129.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2756..>2932
                     /locus_tag="ACHUOF_004215"
     CDS             2756..>2932
                     /locus_tag="ACHUOF_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613130.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELW"
BASE COUNT          659 a          800 c          806 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt gg
//
LOCUS       DAZOYF010000290         2894 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZOYF010000290 DAZOYF010000000
VERSION     DAZOYF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.296"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..308)
                     /locus_tag="ACHUOF_004216"
     CDS             complement(<1..308)
                     /locus_tag="ACHUOF_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR0613131.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     "
     gene            complement(355..1074)
                     /gene="btsR"
                     /locus_tag="ACHUOF_004217"
                     /gene_synonym="yehT"
     CDS             complement(355..1074)
                     /gene="btsR"
                     /locus_tag="ACHUOF_004217"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR0613132.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="ACHUOF_004218"
     CDS             complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="ACHUOF_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR0613133.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          748 a          774 c          657 g          715 t
ORIGIN      
        1 tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg
       61 cgacgttccg gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg
      121 ccattgagga atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct
      181 ttacgtagcc agacggcgat ctgttccgcg gtggcttcga cattatccag cgccagaata
      241 cgcaccaggt cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta
      301 cttatcatga ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag
      361 gccaatcgcc tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt
      421 acgcaaaatc aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag
      481 gttaaccaga tactgacgat ggcagcgcag tagtggtgta cgactttcca aggtacgtaa
      541 tgtcaattcg gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg
      601 actgctgaca aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca
      661 agggataaat ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg
      721 ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag
      781 ataatcaaag gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa
      841 aacaatatac gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg
      901 cggcatctgg atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc
      961 ttccacggcg tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat
     1021 acgcaggttc tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg
     1081 tccctccatg gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt
     1141 attccatagt catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc
     1201 ccactggcat tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa
     1261 tgttgcccct cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc
     1321 ccatgtttaa tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg
     1381 gataattctt gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca
     1441 atttgcagat aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc
     1501 cgctttaagt ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg
     1561 ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc
     1621 acctgggcgt gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat
     1681 tgccctgcaa gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt
     1741 attgaactga ataaccggtt tttggcttca tacaatttga tggtgcccat cacgcgctga
     1801 ttttcgccac gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag
     1861 caacgataag gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt
     1921 aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc aattccggta
     1981 aaggccagca atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac
     2041 agtacctgag ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc
     2101 gtcgaggctg ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc
     2161 gcgcgtttat cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc
     2221 attggcgcag caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgca
     2281 aggatgatca gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg
     2341 ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc
     2401 aatccttcaa cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat
     2461 cgatgtaagc cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg
     2521 cccattaccg cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac
     2581 caggtgccca tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga
     2641 cgaaccgtga cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg
     2701 actaaaaaaa cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc
     2761 gcaaaccaca cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga
     2821 actcttgcag aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata
     2881 aaatcaccct atag
//
LOCUS       DAZOYF010000291         2889 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZOYF010000291 DAZOYF010000000
VERSION     DAZOYF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.297"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="ACHUOF_004219"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="ACHUOF_004219"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFR0613134.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="ACHUOF_004220"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="ACHUOF_004220"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFR0613135.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="ACHUOF_004221"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="ACHUOF_004221"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFR0613136.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..2809)
                     /locus_tag="ACHUOF_004222"
     CDS             complement(1895..2809)
                     /locus_tag="ACHUOF_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFR0613137.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          760 a          667 c          771 g          691 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccagtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgca
     2521 gtaacgaccg tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     2581 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     2641 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag
     2701 caatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     2761 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     2821 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac
     2881 aaattacct
//
LOCUS       DAZOYF010000292         2877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZOYF010000292 DAZOYF010000000
VERSION     DAZOYF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.298"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..593)
                     /locus_tag="ACHUOF_004223"
     CDS             complement(<1..593)
                     /locus_tag="ACHUOF_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR0613138.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHUOF_004224"
     CDS             complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHUOF_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR0613139.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1422..1961)
                     /locus_tag="ACHUOF_004225"
     CDS             complement(1422..1961)
                     /locus_tag="ACHUOF_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0613140.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHUOF_004226"
     CDS             complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHUOF_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR0613141.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT          747 a          714 c          634 g          782 t
ORIGIN      
        1 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
       61 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
      121 aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt
      181 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
      241 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
      301 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
      361 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
      421 ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt
      481 gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg atgcttacga
      541 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
      601 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
      661 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
      721 gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
      781 aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt
      841 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
      901 tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg gctgataatt
      961 gcgagctgta gcatattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
     1021 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
     1081 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
     1141 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
     1201 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
     1261 gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
     1321 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
     1381 ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata ggttaaagag
     1441 aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
     1501 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
     1561 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt ggaggctatc
     1621 cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac accgtgaaac
     1681 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
     1741 accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg ccccatattg
     1801 accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga
     1861 aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
     1921 ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt tatcgcacaa
     1981 gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt gatactgaac
     2041 cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg cataataacg
     2101 cgcctggaac ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc
     2161 accgtccagc gtcagagcat tgcctgttct gtctaggatc tgcacgccaa cgtttgttgc
     2221 actacctgca gcagaactct gcagagccag tacatcagta cgcgtagcat caattgccgt
     2281 acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat
     2341 gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg
     2401 tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
     2461 ttccccttta aagtgaatgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
     2521 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
     2581 ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
     2641 tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc
     2701 cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt tgttggctta
     2761 atattctatt attatcttta tttatagatt tttatattgc atgaggtggt ttttggagag
     2821 aagaatgagg aagatgcgtc gagccacaga aacgttagct ttacatatag cggaggt
//
LOCUS       DAZOYF010000293         2862 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZOYF010000293 DAZOYF010000000
VERSION     DAZOYF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.299"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="ACHUOF_004227"
     CDS             complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="ACHUOF_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR0613142.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
     gene            complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="ACHUOF_004228"
     CDS             complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="ACHUOF_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR0613143.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1929..>2862)
                     /gene="tnaB"
                     /locus_tag="ACHUOF_004229"
     CDS             complement(1929..>2862)
                     /gene="tnaB"
                     /locus_tag="ACHUOF_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFR0613144.1"
                     /translation="SANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAAS
                     RITSLFLGLKIISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFG
                     FHGNIPSLIICYGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGG
                     NIDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGR
                     FKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFT
                     VWGGNLIPAIVILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          833 a          745 c          727 g          557 t
ORIGIN      
        1 caggtgtcgc tctgttccca gcaaatgctg atatgcggat aacgtaaaaa ggtgtccaga
       61 ttccacgttt catgcagtgc cggatgatct ttgcgtaacc agacgcaggg tacatcacta
      121 aacagcactt cataatcaat ggctaacggt agcgagctta acagctcccg cgagcgagga
      181 tggctttcgc gaccggaaaa accaatatcc acttcaccac gagtaatggc atctaaggaa
      241 tcgtaatccc agttacgtag tttgatggtc gcctgcgggt aacgttggta gatccgtttc
      301 gacagcgcat taagcatgat catcatcagc ggcgattccg ccgccaactc aaacttcagg
      361 ccgcgcggtg tctggtggtg cggtttatcc agcagcagat tgctcatttg catccattcc
      421 gccagatttt gctccatgct gaccattagc tgtgtgggcg acagccccag cggtgagttc
      481 acaaagagcg ggtcgtcaaa ccacgctctt aactttgcca gcgacttact caccgctgaa
      541 ggggtcacgt tcatccgctt cgccgctttg gtcacgctgc gctcctgcat cagcagttgc
      601 agacagagca gcagattgag atcaagcgtg gtgatggatt tcttcatgag cggcaacggg
      661 gcgtccaggc gcgacgaaca taatcagcaa caggctcact atgctacagg caatcagaat
      721 cccgatcagc atattccatg cgccgatacc aaccaccgcg gccagccaaa tccacagtga
      781 cgaaccgcaa acctgcgcaa tacctaaggt cgagctggct acgcccgcgc gtaatgaaaa
      841 tggtcctaac gcctgactca tcgctacgcc aaaacctacc gagaaaccgg cgcaaatcag
      901 cgtgatacca aacagagaaa ccgcatggga aggtgaaacg gcaagggtga tccccgccgc
      961 aaggaataac acctgcgagg tgatcatcaa cgtacgtggc ttaaaaattc ccagcgcaaa
     1021 tggcgtcgag aatgaaaagg tcatgctgac gccagcggtc agcgccataa tggtggcgta
     1081 ttctccgcgc tcaaacccca tgatttccat cagcaatacc ggcgatgtat tgacgaaagt
     1141 gaggatcacc gaaacgctga gggtggtgat aacaacacgg ctgaggaaaa aacgattaag
     1201 cagcgactcg ctattttctc gtggtttgtc cgaagcagcg ggggacgctg ggcgcgtttc
     1261 ttttaaaata aacaaagaca acatcagtac cgcgatgccc atcattgcca tcgcccagaa
     1321 cagactctgc cacgggaatt taagcataat cagatgtccg agcactggcg ctaacaccgg
     1381 aatgatgcag gtaataccgt tgagtaatga cagcacttta gcccgacgtc gatcatccag
     1441 cgtgtcgcgc aaaatggcga acgccaccac gtaacaacag cctgcgccca acccctgtag
     1501 aaatcggcct gcaagaaata acgtgctggt ttcagccagt gaacagaaca ccgaggcaat
     1561 aataaatagc gccgcgccgg gtatggcgac cggctttctc cctgaacgat cggccacttt
     1621 accggcaaat agcatcgcag ctgccatccc cgccagatat acggagaacg caatatgcaa
     1681 ctgcgcttcg ctggcattga gatcggcggc gatgcgcggt aaaccaacga ggtacatatc
     1741 aatcccggcg ggataaagta aaaccagggc aaaactacaa atcaaaaagc gggacatggg
     1801 ctaaagcgtc ctgatagtaa ggtgacgcaa agcatacgtg gtgaaggagg ataaggcgag
     1861 ttgccatttg gacataagtg atttcctgag aggcaagaag ccagcgaatg gctggcttct
     1921 tgaaggattt agccaaattt aggtaacacg ttaaagacgt tgccgaacca gcacaaaatc
     1981 acggttatac caaagagaat gacaatcgcc ggaataagat tgccgcccca gaccgtgaac
     2041 atctgattgg gaaacttctt gcgagctttg attgcaagca ctgcgggaat aatgaccgcc
     2101 cagatggtgg cgcacagccc ggcaccgccg atcccgtaaa taaagccgtt cgggaagatc
     2161 agatacaaca acgcaggtgg caggaaggtt aacagcacgg ttttgaaacg cccgccgtgg
     2221 gagttatcaa tcttaaacag gtccgccaga taatcgaaca accccagcgt gacaccaaag
     2281 aacgaactgg caacagctaa gttagagaac accagcaggc aaaactcgat aatgccgtgc
     2341 tgtttggtgc cgaggaacga tttcaccagc gaatcaatgt tgccgcctga ggagattatc
     2401 gccttaaagc tttcgcgcgg aatattcccc atcgtgcaat agagccagaa gagataaatc
     2461 accagcgcca gcagcgaacc aaataccacg cttttgatta acttatcttt gcgtttgcca
     2521 tagcaaataa tcaggctggg aatattgccg tggaaaccaa atgacgccag acacaccggc
     2581 aaagccataa agatatacgg gaagtaagac gttcccgcag tggtgctggt ggcatcgcgc
     2641 aggatggagt aatcgacctg gaagaagaaa gaaccaaaca cgatcacaaa ggagataatc
     2701 ttcagcccga ggaacaatga ggtaatgcga ctggcggcta acgaacttat ccacaatacg
     2761 ctggcaacga aaatggctgt gcagatcccg acaatacgtg gattagcgtg atagcccaaa
     2821 ttcattgata tcgtttcact aatgatcgca ccattagcag ag
//
LOCUS       DAZOYF010000294         2840 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZOYF010000294 DAZOYF010000000
VERSION     DAZOYF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.300"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1556)
                     /locus_tag="ACHUOF_004230"
     CDS             complement(<1..1556)
                     /locus_tag="ACHUOF_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR0613145.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVSGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFV"
     gene            complement(1988..2707)
                     /locus_tag="ACHUOF_004231"
     CDS             complement(1988..2707)
                     /locus_tag="ACHUOF_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFR0613146.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          878 a          544 c          483 g          935 t
ORIGIN      
        1 acaaatacac caggaatacc atctttatta gttgcggaca gcatttcgac caatcgtctt
       61 gtggtaaaac tatagttttt tagaatatta caaaaggcac gtattgtttc ggtatggcca
      121 ttataaaatg ccataaaaag tcctgatgta ccgttgggat tttttatcca taataattca
      181 tctatatatt cctggtgagg ggggagtatt tttagtatat ctccatacgc cataatggcc
      241 tttgtgtgcc cattttgaag tgccataaac aacccatgct ttactttcaa taattcttta
      301 atttgttcac tactaagcat tttcttatta agcagacttc cgaacatacg aatactatct
      361 gcatgtccat tctgcaatgc aaaaaataac ccaggtaaat catggctgtc tttcgcagct
      421 aatagatata atctcttatc ggaagttaac ggggttttaa agactatttc cccataggct
      481 tttattgtgt ctacatgtcc gttcataaaa gacatgcata aacccgacac attgtttgcc
      541 cctaccgcag caagcaatgt aagtattgtt tcttgactaa gattgcacat atttaatatc
      601 ttaccatata cttgaataat gtctgaatat ccgttttgaa gtgctatata aaatgcagga
      661 gtaccatcag agcttctcgt ggccagaagg ttcaccagtt tagattgaga aatttcacca
      721 tttttgtatt tttccatcag tataaataat acttctgtca gattctttgt tatcgcatga
      781 tatataactc tggaattgat caattcatct ggtaaaagtt tcactgtgtt tgttagaggg
      841 gaattatgcc tatcttcaaa tattacctgc ccgggttgct ccagaccata aattttccaa
      901 taaaaatcat caaaaaaatc cttagcggat aattgactca gttcgaaatg gctatcacaa
      961 gtaaatacag tacgtttatg agtaacagtt cgattaggat cgaaaaactg aacaacccat
     1021 ttttcacttt cttgtacgcg ctttattttc aaccctaacg ccatttggtg gtttaatgtg
     1081 catgctgata atgttctgac aaagtctcca ttttttttca tgttataaaa aaaattagta
     1141 ataactgttc cccacttttt attttccaca aaataaatat aagctggtga ttttgaaaaa
     1201 tcttccgttt tttccaaaca tacatttttt gcaatagcat caggggtgct aagaagctga
     1261 tagtttacct tcccatttgt ttccataaaa ttcatttccc aataggaagc taggtgacgg
     1321 caaactattt gtcttccata tatatctttt gccactccat ttatatttac ctccacattt
     1381 ctcttttctg aaaaataagg tgctgtttta ttcttaaact gaattagttc actggagttt
     1441 gagaccctca taatagaact tgttattgtt ctattatctc tgttaacaga catatctgtt
     1501 gataacgaat tttttgttga aaatatacta gatgaaaatg ttttatttaa cggcatagaa
     1561 gacaaccata gagatgttat aatactatta gattgcagtt actaatagta aaagcctcct
     1621 tacaaggagg tagcttattc agaatgctcc tataagatag cgagctcgtc aacgatacag
     1681 atgatctgat tatattgtgc aaaaggagaa caggaaaata ggcactggaa aagatgaagg
     1741 cctgatggga gcattaacta atgcaaaatc cgacaggtta atatcccgaa aagcaatcaa
     1801 acgactcaaa caggagatgc acgctgaaat ggtggacaac tgttcggatt tcagatgagt
     1861 agctcagcag tttatgagcc gaatgctctg tcacgggcag gaatcaggct gcatacgcag
     1921 tgccaaatct aatcgtagtt attctaacca gttttcaagc tacaaggtgg tgtagctggc
     1981 atcttctcta ctctatcatc aaacgataaa atggttcctc tcgtaacatt tgctcttgcc
     2041 tttcacttcc atctcgatcg cttcgtagtt cattacgata gctgacatat ttccagtctt
     2101 caggtctaac atctgacgcc ggatactccc cgggagcgat tttgcttctt aatagattag
     2161 actcgagaca cattataaat tgtcgtattt tatgaagtaa taaagcacta tattgcgagc
     2221 attcctgatc tgattttaca tatagcgtaa actgagctcc tatccccaca cgagattgct
     2281 gagaaactcg attcatatca gttatcttcc atttatctat aggactgtcc tctgaaaata
     2341 ataaaccaga caaaatttgg aatgccagag gaacttgctc cctagctata ctaatatgaa
     2401 acttgtcccc aacaaaatcc cccttactct gaggattttc acgacatgca tgaataaaca
     2461 catctccact atggttcaca atctggaatc cttggtacat gacagaaaaa gattgacata
     2521 tatcagagta agttggcacg ttcgatgcta ctttgaaata gtacgctggg tattgccgta
     2581 tttggttgta caatatattg aagttatttt tcaatctttc attcgcagaa agcatctgcg
     2641 gtatttcaga cctcacaaca ttcaaagaat ctaaatttag ttttagacag ggctttttta
     2701 tgggcataga aaacgtcctc tataaaatag atatattatc ttatattgat tgtttatcat
     2761 cttttataaa acgatacatt atatgtaatg tatgtaagcg taagggtcct ctccgaacat
     2821 ggtgggaagg cttagaacgc
//
LOCUS       DAZOYF010000295         2809 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZOYF010000295 DAZOYF010000000
VERSION     DAZOYF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.302"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..98
                     /locus_tag="ACHUOF_004232"
     CDS             <1..98
                     /locus_tag="ACHUOF_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0613147.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="ACHUOF_004233"
     CDS             156..512
                     /locus_tag="ACHUOF_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613148.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            605..823
                     /locus_tag="ACHUOF_004234"
     CDS             605..823
                     /locus_tag="ACHUOF_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR0613149.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            825..1190
                     /locus_tag="ACHUOF_004235"
     CDS             825..1190
                     /locus_tag="ACHUOF_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0613150.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            1187..1852
                     /locus_tag="ACHUOF_004236"
     CDS             1187..1852
                     /locus_tag="ACHUOF_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613151.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1852..2217
                     /locus_tag="ACHUOF_004237"
     CDS             1852..2217
                     /locus_tag="ACHUOF_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0613152.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2672..>2809
                     /gene="hokD"
                     /locus_tag="ACHUOF_004238"
     CDS             2672..>2809
                     /gene="hokD"
                     /locus_tag="ACHUOF_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0613153.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TA"
BASE COUNT          792 a          579 c          750 g          688 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatcagga gaccgcccga aagggcggta
      601 atgaatggtt acattattta gaaaaaaata tccgcgaaag agtagaacaa cagaattcct
      661 gtttctcatt ctgtttatcg tgttgatgac accgatatcc ccgcaaattt ttgtctggat
      721 aatcggaaaa ataattgagc cagttattga attgtataac gacgtggtat gggcgtcatt
      781 caacacactg cacaataaaa ttaatccgta taaggaaagc tgatatggca ctgacgaaaa
      841 aacaacgtgc agaactgcgc atgaagttcg gtggtcgctg cgcttattgc ggctgcgaac
      901 ttggcgaaaa gtggcatgca gaccatgtaa aaccggtcat tcgttttgat ggaaatatgc
      961 ttcaccagga acgtgacgat atatccaaca tggtaccaga atgccaccca tgcaatctgc
     1021 acaagcattg cagtagcctg gaagattatc gacgaattat cagtgatggt cgtcgtgaat
     1081 tccttgcgtc cgggaaaggc aaagcgctgg ttcgtatggg attggttgaa atgaaatctg
     1141 acccggttgt gttctggttt gaaatatatc aagaaggggc tacggcatga ctactattac
     1201 caaagagcga ctgcaatggc tggctaacat ttctggccgc gatgatattg acgatataga
     1261 cggcggtgaa attcgtgaac tggcgcttat cgctctggca tcactggaag cagagcctgt
     1321 agcggaatgc attgttgaag atgggggcat gtgtattgat gggttcggtg agtatgtggg
     1381 tcactcgctg cctgatggaa tgcatgagct ttatgctgcc ccgcgaatgc gacaacctga
     1441 tggatggaaa gccgtagctg tagcgtggaa ggtgacgttt actcaagttg accaggaatc
     1501 taatacgttc actgctatat atttttacaa agcggaagtt gagcgttggg tacgactgca
     1561 taaagcatgt gattttcggg cagatataac accgctttac gcagcgccgg cagtgccggt
     1621 tgcagtaaac gacgacatgg cttacgcatt ccatcacgca ctgtcagatt catcgcttgg
     1681 ctctgatgaa atcgaagaaa ttaaaaccgg tttgcgtgct gcctttgcca acgtcactat
     1741 ccaaccagag ccgatagtgc cggatgaaat cgggccaaac gatagtaata cgtttgatta
     1801 tgttgatggc tggaacgcct gccgcgctgc catgcttaag ggagataaat aatgattaat
     1861 cgaaccaaac tggagcacat tctcgaatat gccaggcagc agaaatgtat tgggcaactt
     1921 tgtaaaattc caccaggaga tatggttgaa atcgtggaaa tggccatgcg taaggctggc
     1981 aactctccgg taactctggc tggctggata agctgtagtg atgcagttcc tgctgaatat
     2041 tgcgatgtga ttcttctcga tgatctcggg aatgtattcc ccggttcctg ggataaggtt
     2101 ttttgcccca ctcgtggcgg gaataagatg gcttttgtgg acaaagacgg cgttgaagta
     2161 gagagctcaa ctcactggat gccgctaccg gaaccaccgc aggaggtgaa tcaatgacct
     2221 ggcctgaggc attcaccacg gtaggaattg cgatggcggt ggcgctggtg gtgtattcga
     2281 tttgccgttg gggttaacaa acaaaaaccc cggattgatg gtccggggtt tttgaaggaa
     2341 acaaaacaga aacagcaatt gccgttacct gttgttacca tggcaagtaa acgtatctca
     2401 ggcgagcgca ttgcgccgtt ctgacgcaga taaattagcc tggatagaag gtgctggcaa
     2461 taaaaaatag cgttttctta tcggtatcgg taagattgct gcgggtgctt gaggctgtct
     2521 gcctcgggca tgccactgta aggcagacag agaaaagccc cagttaacat tacgcgtcct
     2581 gcaagacgct taacattaat ctgaggccca atctatgtct cacaaatgta ggttagcctc
     2641 ttacgtgccg aaaggcaagg agaagcaggc tatgaagcag caaaaggcga tgttaatcgc
     2701 cctgatcgtc atctgtttaa ccgtcatagt gacggcactg gtaacgagga aagacctctg
     2761 cgaggtacga atccgaaccg gccagacgga ggtcgctgtc ttcacagct
//
LOCUS       DAZOYF010000296         2808 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZOYF010000296 DAZOYF010000000
VERSION     DAZOYF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.303"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHUOF_004239"
     tRNA            6..81
                     /locus_tag="ACHUOF_004239"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHUOF_004240"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHUOF_004240"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHUOF_004241"
     CDS             complement(299..1057)
                     /locus_tag="ACHUOF_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR0613154.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHUOF_004242"
     CDS             complement(1065..2168)
                     /locus_tag="ACHUOF_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR0613155.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2808)
                     /locus_tag="ACHUOF_004243"
     CDS             complement(2178..>2808)
                     /locus_tag="ACHUOF_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFR0613156.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          740 c          675 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaac
//
LOCUS       DAZOYF010000297         2725 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZOYF010000297 DAZOYF010000000
VERSION     DAZOYF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.304"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(151..1575)
                     /gene="patD"
                     /locus_tag="ACHUOF_004244"
     CDS             complement(151..1575)
                     /gene="patD"
                     /locus_tag="ACHUOF_004244"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR0613157.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1597..2391)
                     /locus_tag="ACHUOF_004245"
     CDS             complement(1597..2391)
                     /locus_tag="ACHUOF_004245"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0613158.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2381..>2725)
                     /locus_tag="ACHUOF_004246"
     CDS             complement(2381..>2725)
                     /locus_tag="ACHUOF_004246"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0613159.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT          698 a          805 c          639 g          583 t
ORIGIN      
        1 aatcaactgt gacaggatga aaaatcgcca tcaaacattt agcctaattt ttacgccagt
       61 tattaatgta atcattctaa tacttaaccg ccattaaact cagaacctaa aacgatagtg
      121 gccgtcctat aacagccact aacatctaac ttaatgttta accatgacgt ggcggacgac
      181 ggtgtaatcc tccagcccat aaagtgacat atccttgccg taaccagaaa gtttctgccc
      241 accgtgcggc atttcactta ccagcatgaa atgggtattg acccaggtac aaccatattg
      301 cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc gtccataccg aagatgcaag
      361 tccgtactgg ctgtcattcg cccagttcac cacctgttct tcgttgtcga agggcgtaac
      421 actcactact ggaccaaata cctctttttg cacgatggca tcgtcctgta atgcgccagc
      481 cagcagcgtc ggcgcatagt aatagccatt acccttgcgc ttttcaccgc cagtgatcac
      541 tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg ctgacgcgtt cgagatgcgc
      601 cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc ggcgcaccag attttaacgt
      661 tgccaccgca gcacccagtt tttccaccag cgtatcgtaa atgccttttt gcgcgtagat
      721 ccgacaagcc gcagtacaat cctgtccagc attgtaatag ccaaatgtac gtacaccttc
      781 gaccactgct tcaatatccg catcatcaaa aacaatcact ggcgctttgc caccaagttc
      841 catatgagta cgcttaatgg acgacgcggt atggctgatg atgtgctcgc cggtggcgat
      901 agagcccgtc agcgacacca tccgcacttt gggatgaccg gtcagcggat cacccaccgt
      961 tttgcctctg ccaaacagta tgttaatcac gcctgccggg aagatatctt tcgccagctc
     1021 tgccaacttc aacgcggtca gcggggtaat ttctgatggt ttaagcacta cgcagttccc
     1081 tgccgccagc gccggagcaa gtttccacgc ggccatcatc agcggataat tccacggtgc
     1141 gatagaagcc acgaccccca acggatcgcg acggatcatc gaagtatgac cttcaagata
     1201 ttcacctgcc gccagaccat tcagacagcg cgccgcaccc gcgaaaaagc gaaaaacatc
     1261 gacaatcgcc gggatttcat cattgaacgc actatgcagc ggtttgccac aattacggga
     1321 ttccagttcg gcaaaaacct gaccattttc ttcgataaca tccgccagtt tcagcaggca
     1381 ttccgcacgc gctttcggtg tggtttgccc ccattcagca aatgctgcat ctgccgcacg
     1441 cacagcagca tcgacctgct ctgcggatac ctcggcaatc tccagtaaaa cgtcccccgt
     1501 tgccggatta tagacaggct gtttttcccc ttcgccgcta acccgttctc cgttaatcag
     1561 taacttatgt tgcatagctt attcctgtat cagtggttat tgaccattgt cgccttcgcg
     1621 ggttagccac caggccccca ggatcggcaa ggttgttacc aacataacca gcagtgccac
     1681 cacgttggtt accggtacat cacgcggtcg cccaagctga ttgagcaacc acaacggtaa
     1741 cgttcgttca tgacctgccg taaaggtcgt aacgatgatt tcatcgaacg acaaggcaaa
     1801 cgccagcatt cctcctgcca gtaacgccga actgagattc ggcaacacta cgtagcggaa
     1861 ggtttgccag ccattggccc caagatccat tgacgcctca accagactcc acgaggtgcg
     1921 gcgaaaacgg gcgatgacat tgttaaacac caccactacg caaaaagtcg catgaccgac
     1981 cacgatggtg aaaaatcccg gctccagatt gatggtttta aaggcggtta atagcgccag
     2041 accagtgaca atgcccggca gcgcaatggg cagcagcaat aacagcgaaa tggcgttttt
     2101 gccgaaaaag tctcgtctcc acagcgcggc agctgccagc gtccctaaca ccagcgcaat
     2161 taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg gcatcaagaa tatcactacg
     2221 ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc ggtggaaaac taaacgccgc
     2281 atcttcagtg ttaaacgcat aggcggcgat tatcaggatg ggaaaatgta ggaaaacaac
     2341 gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt tcagagtgca tcgaacgctc
     2401 ccagacgttt cacgaacgcc aggtacagtg cgatgagaat aatcggcacc agggtgaatg
     2461 ccgccgccat cggcatattg ccaatcgccc cctgctggga ataaaccata ttgccgataa
     2521 aatatcctgg aggaccaacc agctgcggga cgataaaatc gcccagtgtg agtgagaagg
     2581 taaagataga gccagcggca atacccggga ttgccagcgg cagcaccaca tagcgaaagg
     2641 tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa caatgacggc ggcaaacgct
     2701 caagcgccgc ctgaacgggc aggat
//
LOCUS       DAZOYF010000298         2715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZOYF010000298 DAZOYF010000000
VERSION     DAZOYF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.305"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            53..2575
                     /gene="lpfC-O113"
                     /locus_tag="ACHUOF_004247"
     CDS             53..2575
                     /gene="lpfC-O113"
                     /locus_tag="ACHUOF_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR0613160.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2586..>2715
                     /locus_tag="ACHUOF_004248"
     CDS             2586..>2715
                     /locus_tag="ACHUOF_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0613161.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          727 a          586 c          679 g          723 t
ORIGIN      
        1 cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac
       61 gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat
      121 ggccgaggtc agtgtattca atccggcgct tctggaaatc aaccatcaat ccggagtcga
      181 tattcgccag tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt
      241 tatcaacggt aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc
      301 tgatctgcac gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt
      361 tgatgcgctc aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg
      421 tattgaaggc tcatcctggc agtttgacag tgataaattg cagctgaata tatccattca
      481 ccaaatctac atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg
      541 gattaatgcg ctcaccatca actacgattt ttctggttca catacactac gttcagatta
      601 tggttcacaa gagacagata ccagttatct caatctgcgc aatggactga atattggacc
      661 gtggcggcta cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc
      721 cattagtacc tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga
      781 tacgtggacg gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac
      841 tgataacgat atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc
      901 aaagtccaac gccaccgtca tcattcggca gaatggctac gtgatttatc agtcagccgt
      961 tccacaaggt gcttttgaga tcaccgatct caacaccgca agtacaggtg gcgatttgga
     1021 cgtaaccatc aaagaagaag acggtagcga acaacgattc acccaacctt atgcttcatt
     1081 ggcgattctt aaacgtgaag gtctgacaga tgttgatgtc agcgtgggtg aattgcgcga
     1141 tgaagacgga tttacaccgg acgtccttca ggcgcaaata cttcatggtt tttcccacgg
     1201 gatcacttta tatggaggta tgcaggctgc tgaaaattat ggttctgcag ctctgggtgt
     1261 cggtaaagat cttggcgctt tgggcgcaat ttctttcgat gtgacacatg ctcgtgcgaa
     1321 ttttagccat gatgatacag aaacgggtca gtcatatcgc tttctctatt caaaactatt
     1381 tgacgacaca gacactagct tgcgcctggt tggctatcgt tactccaccg agggctacta
     1441 taccctcaat gagtgggcat cgcggcgcaa cagccctgaa gacttttggg aaacaggtaa
     1501 ccgacgtagt cgcgtggagg gaacgctaac gcagtcgttg gggagagatt atggcaattt
     1561 atacctgaca ttaagccggc aacaatactg gcataccgat gatgtcgaac gattaatgca
     1621 atttggctac agcagtagct ggaagcgtct ctcgtggaac gtctcctgga gttattccaa
     1681 tactgccaga caggggacgg ggaacaacca tgccagtgat aacaccagtg agcagatcta
     1741 catgctctct ttatctgttc ctttatcggg ctggtggggt aatagttacg ccacctattc
     1801 tgtttcgcaa aacgataatt ccggtagctc acatcaactc ggactcagcg gtacggcgct
     1861 ggaaagaaat aacctttcat ggaatttaat gcagtcctat aacagtcatg atgatgaggt
     1921 tggcggtaat atgtccctga cctatgatgg ctcttatggc acggtgaacg gcagctataa
     1981 ctacagccaa aattcccaga ggctgaatta tggtatcaga gggggaattc tggcacacag
     2041 cgaaggggta acgttaagtc aggagttagg tgaaactatt gctcttgtta aagcacctgg
     2101 ggccgccagg ttagaaatag ataatatgcg cggtgctgcg acggactggc gcggctatac
     2161 ggtcaagaca cagctaaacc cttatgatga aaatcgggta gcaatcagcg ataactattt
     2221 ctcgaagtcg aatatagaac ttgataatac cgtcgttacg atggttccca cgcgtggtgc
     2281 agtggttaaa gcggagtttg tgactcatgt gggttatcgc gttctcttca gagtgttaaa
     2341 tgcaaatggt aaaccggtac cttttggagc cattgctgcg atacaagatg caagtttggc
     2401 agattcagga attgtcggtg accgtggcga actttatctt tctggtctac cagaaaaagg
     2461 acaggttacg ttatcctggg gagaaaacgc ctcaacaaaa tgcatcttca attattcatt
     2521 ttcgacacca gaaagtgaga gcggattaat tgaacagggt gtgacatgtc attaaaagga
     2581 tttttatgaa caagtatatc aaacagtggt gctttgctgt gtttatgctc tcgttaagta
     2641 gtgtagcact tgcagctcct aaaggtatct gtacccctga taatggcgta tttcacagta
     2701 cgcttgattt ctcgg
//
LOCUS       DAZOYF010000299         2692 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZOYF010000299 DAZOYF010000000
VERSION     DAZOYF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.306"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..893
                     /locus_tag="ACHUOF_004249"
     CDS             <1..893
                     /locus_tag="ACHUOF_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR0613162.1"
                     /translation="ELARVGVNPHDVAQMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQ
                     DKMQGRQVTMRGQDLDSQTAARNQAITMRGQDIQANLGQQRINLDAETNRINNENKRL
                     DRMLSAETNDLKRQEIQSRIAANNQQLQQKQQALNDGYKDGINTLTTSMFTLNDIVSS
                     PSLKSITGLRGVIPNVPGSQAADTQARLDTFKSQAYLTAVQAMRGMGALSDAEGKKLD
                     QAVGSLQNSQSEESFRRNAGVILNTLNQKRNEAVGKYVQQNGIKRVEAPQASIDYLKQ
                     HPELSIDFINRYGYLPSLGQ"
     gene            894..2624
                     /locus_tag="ACHUOF_004250"
     CDS             894..2624
                     /locus_tag="ACHUOF_004250"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR0613163.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT          757 a          661 c          707 g          567 t
ORIGIN      
        1 gtgagttagc tcgtgttggc gttaatcctc atgacgtcgc tcagatgtac caacagaacc
       61 cgcggcagtt cggcgaattt gtcgatcacc tggggatgaa cagtctcggg cccgaaaaat
      121 actttgacct acaggataaa atgcagggtc gccaggttac catgcgcggt caggatctgg
      181 attcgcaaac cgccgctcgg aatcaggcaa tcacaatgcg cggacaagat atccaggcga
      241 atttaggtca gcagcgcatt aatctggacg cagaaacaaa ccgcattaac aacgaaaata
      301 agcgccttga ccggatgcta tcagcagaaa ctaacgacct gaagcgccag gaaatacaga
      361 gccgcatagc agccaacaac cagcagttgc agcagaagca gcaagcgcta aatgatggct
      421 acaaagacgg catcaacacc ctcacaacca gcatgttcac tctgaacgat atcgttagtt
      481 ctccttcact taagagcatt acaggcttac gtggagtaat ccccaacgtt ccaggctcac
      541 aggctgcaga cactcaggca cgacttgata cctttaaatc acaagcatac ctgacagcgg
      601 ttcaggccat gcgaggcatg ggcgcacttt ctgatgccga gggcaaaaag ctcgaccagg
      661 ctgttggttc gctgcagaac tcgcagagcg aggagtcctt tcgtcgcaac gctggcgtca
      721 tcctgaacac gctcaaccag aagcgtaatg aggcggttgg taagtacgtt cagcaaaacg
      781 gtatcaagcg agtggaagcg cctcaggctt ctatagatta cctgaagcag caccccgagc
      841 tgtcaatcga cttcattaat cgctacggat atcttccatc tttggggcag taaatggcta
      901 attaccgtga tttgttagag caggctggcg cacgttacgg tgtgccagaa gggttgatga
      961 ctgcactggg tgccaaggag tcttcttaca accctgccgc agtaagctcc gccggggctg
     1021 taggattgac tcaggtcatg cctgggacat ggcgtgatat gggttatacc gatgagcaaa
     1081 tgcaaaaccc cgaatatcag gctgacgctg gcgcgcgcta tctggcaaag atgtaccagc
     1141 agtttggtaa ctggcgtgac tctcttcagg cttatcacga cggtcccggc aacgttatga
     1201 aggcaaagcg tggtgaatat acgccaggac ctgaaggccg cggttacgtt gatgatcgct
     1261 ttgctcaatg ggctggtgac ccggtgacag actcaacagt cgaacagcgc gccacctctg
     1321 caaaggtaca tcctcagcaa gaccctaaca acccgtttgc acaactggaa gtacagtcat
     1381 ccgaacaagt atcggcatca ggtgtgcagt cagacccaaa taatccattt gctcagattg
     1441 agcagcaggc agccagtcag cagccacctc aacccgtaag ttctgtcgca ccgaaacctg
     1501 ttcagcaaca aacagttaat caggccaata atgcaccaac acgggaagaa ccatcattga
     1561 tgcaacaagc tggcgattgg ctcactggtg gtcaaagtgc agggcaaatt gcagaacagg
     1621 ctggtcgtgg tctggtaaac ataccatttg acgtattgca gggtggcgca agtctgatta
     1681 atgcaatcag tcaggggctt ggggggccaa aagtattgga tgatgtttat cgtccagtag
     1741 acagaccgac agacccctac gcgcaagctg gagagtcaat aggcggttat cttgttcctg
     1801 gagcaggtgt agctggaaac atggtcattg gttctctcgc tgacgcggcg aatcaacggg
     1861 gtgattttgc cgaaaatgcc gctattaatg ccggacttag cattgctacg catggcctga
     1921 taaatggcgt tacccgtggt gttcgtggtg catcaaatat aattagtggc aataaaacat
     1981 ctgcacagag agcgaccact gcgccaacag aaacatcacc attctccggt gatgccgctg
     2041 cagcaacaaa tcctgcggtt catgccgcag aggcaagagt agcacaaggt gtaccaatga
     2101 cgcctgcgac gaagaaccca gaggaagtcg ttcgcacagt agcagcacaa aaaaggccaa
     2161 atctcgcttc atcgcttgat gaactagata tcaatcctca ggctgaagtt ctggagtctg
     2221 ctgaaaggct taatgttgat tcattactcc cttcacactt ttccgggaac gagcaataca
     2281 aggcagttga gcaagcaatc aagtcccgtg cgggttctgc tctacaggtg caggaaaatg
     2341 aagcaatcag gcagctagca cagggcgcgg gtgagataat tgatcgcgtt tccggtgcaa
     2401 aagatgctct tggtatgagc gacaagttta ttgatacggt caatggaaga atgtctgcgc
     2461 tgatgaaacg aagcgaccag ctttatcgca atgttgaaaa ggcgatgcct gcaggtgcaa
     2521 aaattgatgc gccatcaaca aggtcaatgc tcaaacaggt ggcagaagat cttggcggga
     2581 tgaaaaacct tgaccctatt gaaaagagag ttgaggtagc ctgagtttaa cggacactcc
     2641 ttcctgaaat agaatggcat cagaaggagc taataatgag cagaaaaacc ca
//
LOCUS       DAZOYF010000300         2681 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZOYF010000300 DAZOYF010000000
VERSION     DAZOYF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.307"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            170..1273
                     /gene="proB"
                     /locus_tag="ACHUOF_004251"
     CDS             170..1273
                     /gene="proB"
                     /locus_tag="ACHUOF_004251"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR0613164.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1285..2538
                     /gene="proA"
                     /locus_tag="ACHUOF_004252"
     CDS             1285..2538
                     /gene="proA"
                     /locus_tag="ACHUOF_004252"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR0613165.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT          637 a          678 c          769 g          597 t
ORIGIN      
        1 tgtggtaatt tattaaatct gtaataaaag cgtaaacaac tgccgctacg cttgctgatc
       61 ccgcgcaaca aaacgccatg ctttgctcgc agatggttgg caaccgacga cagtcctgct
      121 aaaacgttcg tttgatatca tttttcctaa aattgaatgg cagagaatca tgagtgacag
      181 ccagacgctg gtggtaaaac tcggcaccag tgtgctaaca ggcggatcgc gccgtctgaa
      241 ccgtgcccat atcgttgaac ttgttcgcca gtgcgcgcag ttacatgccg ccgggcatcg
      301 gattgttatt gtgacgtcgg gcgcgatcgc cgccggacgt gagcacctgg gttacccgga
      361 actgccagcg actatcgcct cgaaacaact gctggcggcg gtagggcaga gtcgactgat
      421 tcaactgtgg gaacagctgt tttcgattta tggcattcac gtcgggcaaa tgctgctgac
      481 ccgtgctgat atggaagacc gtgaacgctt cctgaacgcc cgcgacaccc tgcgtgcgtt
      541 gctcgataac aatatcgttc cggtaatcaa tgagaacgat gctgtcgcta cggcagagat
      601 taaggtcggc gataacgata acctttctgc gctggcggcg attctggcgg gtgccgataa
      661 actgttgctg ctgaccgatc aaaaaggttt gtataccgct gatccgcgca gaaatccgca
      721 ggcagaactg attaaagatg tttacggcat tgatgacgca ctgcgcgcga ttgccggtga
      781 cagcgtttca ggcctcggaa ctggcggcat gagtactaaa ttgcaggccg ctgacgtggc
      841 ttgccgtgcg ggtatcgaca ccattattgc cgcgggcagc aagccgggcg ttattggtga
      901 tgtgatggaa ggcatttccg tcggtacgct gttccatgcc caggcgactc cgcttgaaaa
      961 ccgtaaacgc tggattttcg gtgcgccgcc ggcgggtgaa atcacggtag atgaaggggc
     1021 aactgccgcc attctggaac gcggcagctc cctgttgccg aaaggcatta aaagcgtgac
     1081 tggcaatttc tcgcgtggtg aagtcatccg catttgcaac ctcgaaggtc gcgatatcgc
     1141 ccacggtgtc agtcgttaca acagcgatgc attacgccgt attgccggac accactcgca
     1201 agaaattgat gcaatactgg gatatgaata cggcccggtt gccgttcacc gtgatgacat
     1261 gattacccgt taaggagctg gccgatgctg gaacaaatgg gcattgccgc gaagcaagcc
     1321 tcgtataaat tagcgcaact ctccagccgc gaaaaaaatc gcgtgctgga aaaaatcgcc
     1381 gatgaactgg aagcacaaag cgaaatcatc ctcaacgcta acgcccagga tgttgctgac
     1441 gcgcgagcca atggccttag cgaagcgatg cttgaccgtc tggcactgac gcccgcacgg
     1501 ctgaaaggca ttgccgacga tgtacgtcag gtgtgcaacc tcgccgatcc ggtggggcag
     1561 gtaatcgatg gcggcgtact ggacagcggc ctgcgtcttg agcgtcgtcg cgtaccgctg
     1621 ggggttattg gcgtgattta tgaagcgcgc ccgaacgtga cggttgatgt cgcttcgctg
     1681 tgcctgaaaa ccggtaatgc ggtgatcctg cgtggtggta aagaaacctg tcgcactaac
     1741 gcggcaacgg tggcggtgat tcaggacgcc ctgaaatcct gtggcttacc ggcgggtgcc
     1801 gtgcaggcga ttgataatcc tgaccgtgcg ctggtcagtg aaatgctgcg tatggataaa
     1861 tacatcgaca tgctgatccc gcgtggtggc gctggtttgc ataaactgtg ccgtgaacag
     1921 tcgacaatcc cggtgatcac aggtggtata ggcgtatgcc atatttacgt tgatgaaagt
     1981 gcagagatcg ctgaagcatt aaaagtgatc gtcaacgcga aaactcagcg tccgagcaca
     2041 tgtaatacgg ttgaaacgtt gctggtgaat aaaaacatcg ccgatagctt cctgcccgca
     2101 ttaagcaaac aaatggcgga aagcggcgtg acattacacg cagatgcagc tgcgctgaca
     2161 cagttgcagg caggccctgc gaaggtggtg gcggttaaag ccgaagagta tgacgatgag
     2221 tttctgtcat tagatttgaa cgtcaaaatc gtcagcgatc ttgacgatgc catcgcccat
     2281 attcgtgaac acggcacaca acactccgat gcgatcctga cccgcgatat gcgcaacgcc
     2341 cagcgttttg ttaacgaagt ggattcgtcc gctgtttacg ttaacgcctc tacgcgtttt
     2401 accgacggcg gccagtttgg tctgggtgcg gaagtggcgg taagcacaca aaaactccac
     2461 gcgcgtggcc caatggggct ggaagcactg accacttaca agtggatcgg cattggtgat
     2521 tacaccattc gtgcgtaaat aaaaccgggt gatgcaaaag tagccatttg attcacaagg
     2581 ccattgacgc atcgcccggt tagttttaac cttgtccacc gtgattcacg ttcgtgaaca
     2641 tgtcctttca gggccgatat agctcagttg gtagagcagc g
//
LOCUS       DAZOYF010000301         2642 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZOYF010000301 DAZOYF010000000
VERSION     DAZOYF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.308"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..967
                     /locus_tag="ACHUOF_004253"
     CDS             <1..967
                     /locus_tag="ACHUOF_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0613166.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1548..>2642
                     /locus_tag="ACHUOF_004254"
     CDS             1548..>2642
                     /locus_tag="ACHUOF_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613167.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEK"
BASE COUNT          869 a          525 c          475 g          773 t
ORIGIN      
        1 agagatgttt gtgtttctcg ccgtcgagct ttcgttgctg tttatagtga ttagtgccgg
       61 tgtcagcctg ataagacaaa aggtgccaga ccataaaatc cagcagatga tgggggccag
      121 aaaagggaga ggctatctcc tggctgctct gttgggagcc gttaccccgt tctgtagttg
      181 ctcgacaata cccatgttac gtggattgtt gtcagcgaaa gccgggtttg gtccgactct
      241 cactttttta tttgtttcac cattacttaa tcccattatc gtcggactaa tgtggatgac
      301 ctttggttgg aaagtcacct tgttgtacgc gattatcgca gccggcgtct ccgttcttgc
      361 cagtattatc ctggattctc tggggtttga acgtcatatc attgccagta aaagctcatc
      421 agcaaattgt tgtgctccag caaaaacttc gccgggaacg acatatacgc ccataaaagt
      481 gagttgttgt agtccagcgg ctaaagccat tgagaacccc gtagttacct gttgcagtac
      541 caaagctgta gtaagtatta atcccataaa actagccacc aaagatgcgt tgcaacagtt
      601 taaagatgta ctgccatatc ttttgttagg agtattaata ggatctttta tttatggctt
      661 tattccctca gcgtggattg ccgctcatgc aggagcagat aatcccttcg ccatcccatt
      721 aagcgccgtt gtcggtattc cgctgtatat ccgggctgag gcggttatcc ctctggcatc
      781 tgttctgatg acaaaaggaa tgggtctggg agcattaatg gcattaatca tcggcagtgc
      841 cggcgcaagc ctgacggaag tgatattact taaatcaatg ttcagaatgc cgatgatagc
      901 tgcattcctg acggttatat taggtatggc tatcttgatg ggctatttga ctcaattgct
      961 attttaaata gatgccagca gtaaactata agatgcatag ttagtgccat atccttctaa
     1021 gtagaaaatc atagacagga taatccttaa aaatcacaaa tgatttaact aatgcattga
     1081 gaatgatgac ggctcggtca acaagtcatc aacagacaat aaccacgtaa tcatgcttct
     1141 ctgacatttt atcggagaag caatgactct ttgtctcagt accctcctcc tttttaaaag
     1201 gcagatttca gtcgctaata caacacattt ttaaatacac tttaacctgg ttcattatta
     1261 cacatctaaa acatcacaaa aatattactt cacacactat cggagaacat aataactttt
     1321 taaaatgagc gttttttgat agttgttaac gttttccttt acgatactat cactgccctt
     1381 tttttataca caaatattac cgggtggaaa tgaaagggaa tttaaaggta attatatcag
     1441 gaagatataa cgcattatat ttattgtgtg tagaagaaaa tccattcctg ttctaattat
     1501 ccaatttaaa aacattagca taaaacagat gatgaataag gaatgttatg tcaattgact
     1561 ttaccccagg tataataaat acatatcacg gcgatattta taactgcaca acaaataccg
     1621 ataatgttaa aacgccagac acaccgaagt ggccttgtga taattgggaa gaacaacaac
     1681 ccattaattc cacattctct ggagaaggat acaaccctga acaatttgat ttagcgcaac
     1741 aacaactcca acaaattaat gcatgccaca cgaacaccac atatacaaat gcagactact
     1801 caaaagttgt ggttcaactt gtaagtctta ttaataacat tgaaacgata agttcgacac
     1861 cacttacgca acagacacaa tcaatactta atcagattca taacattaga tacgaaaagg
     1921 ataaggattc tgtatgtcaa atcattgttg tcgttaatcc agaacccaac aatccaatca
     1981 taactaaaat tctcgtagaa gaaggaatac ccgaaagatt cagtgtccaa accgtttctt
     2041 ccgataataa aaattttgct gggcaacgag ctgacttacc aactgatata agagacattc
     2101 aaagcttgta cttaaaaatg gcgaagctgt atattgagca cagtaaaaat gaaaatagga
     2161 tggaagccct tgctggatgc gactttattg atttcaacat gacagggcaa gatatgtcca
     2221 agctagtcct aaccttatca aaattttatt ttgaagatct acttaatata aatttcactg
     2281 acgcaaattt atttaacact attttttcac acaaggaaaa tcccatcccc aaactacata
     2341 aatatgaaca acatcttgac aaacaaatca atggtttgtt ttctacattg ttaactatta
     2401 atgacaactc tttacgcgcg aaagcggaaa ttgcttcgag aataattgat ttcttagagg
     2461 caaaggtagt gaatctttca tttgatgaca tattaaaata taagcaagag ttcaaaaaaa
     2521 tatgttataa acaacttcaa gagtttacaa caccctctct atacaataaa attcaaaaat
     2581 gggcaacaat gtctaaaaat gaattcatag aatttcatta cgagacactt caacccgaaa
     2641 ag
//
LOCUS       DAZOYF010000302         2565 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZOYF010000302 DAZOYF010000000
VERSION     DAZOYF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.310"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..681
                     /gene="ospC1"
                     /locus_tag="ACHUOF_004255"
     CDS             <1..681
                     /gene="ospC1"
                     /locus_tag="ACHUOF_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFR0613168.1"
                     /translation="DKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMKLGLGLYLIDFIR
                     KSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKKVKIREISLEEAV
                     TASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQDVISIKHELYDI
                     EYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYENAEMIKLLLKYG
                     ATSDNKYI"
     gene            complement(785..934)
                     /locus_tag="ACHUOF_004256"
                     /pseudo
     CDS             complement(785..934)
                     /locus_tag="ACHUOF_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1010..>2565
                     /gene="ospD3"
                     /locus_tag="ACHUOF_004257"
     CDS             1010..>2565
                     /gene="ospD3"
                     /locus_tag="ACHUOF_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR0613169.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIA"
BASE COUNT          890 a          433 c          493 g          749 t
ORIGIN      
        1 gataaatttt cagaggttaa taaagaagtt agtcgatacg tacatggaag taaaggaatt
       61 atagatgtac caatattcaa tactaaagat atgaagttag ggctcggatt atacctgatt
      121 gactttatta gaaaaagtga agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt
      181 gcccctgtgg atctggatag aatcataaac tttgtttttc agccagagta ccatatacct
      241 aggatggtaa gtacagaaaa cttcaaaaaa gttaagatta gagaaatatc cttagaggag
      301 gctgttacag catctaatta cgaagaaatt aacaagcagg tcactaacaa aaaaattgct
      361 ctccaggctc tttttctttc gattactaat caaaaagagg atgtcgcctt atatatatta
      421 tctaattttg agataactag acaagatgtt atttccataa agcatgagtt gtatgatatt
      481 gagtatctac ttagcgctca taattcaagc tgtaaagtac ttgagtattt tatcaataag
      541 ggattggttg atgtaaacac aaagttcaaa aaaactaata gtggggattg tatgttggat
      601 aacgcaataa aatatgagaa tgcagaaatg ataaaactat tattgaaata tggtgcaaca
      661 tctgacaata aatatattta atcaaaattg aatatcgttt agcaaaaggc agtatttgca
      721 caaaatatcg atatattgtt tattgtcagt atggctcaat gtgataatag ttggaaagtt
      781 tgatgggttt cgccccgttg tagcggtagt cgaactggaa ggtcttcagg cactgcttac
      841 agcgatagag cagcccccct gaactggaat ggccgttccg gatacccccc tgagtttcag
      901 agtaacggtg acaaaccaca tcaatctttg ccatcaatca tccaaagggc aaagagtaca
      961 acaacactaa gtctgcgtca caacccatca atgaaaggaa tatatacata tgccatcagt
     1021 aaatttaatc ccatcaagga aaatatgttt gcaaaatatg ataaataaag acaacgtctc
     1081 tgttgagaca atccagtctc tattgcactc aaaacaattg ccatattttt ctgacaagag
     1141 gagtttttta ttaaatctaa attgccaagt taccgatcac tctggaagac ttattgtctg
     1201 tcgacattta gcttcctact ggatagcaca gtttaacaaa agtagtggtc acgtggatta
     1261 tcatcacttt gcttttccgg atgaaattaa aaattatgtt tcagtgagtg aagaagaaaa
     1321 ggctattaat gtgcctgcta ttatttattt tgttgaaaac ggttcatggg gagatattat
     1381 tttttatatt ttcaatgaaa tgatttttca ttccgaaaaa agcagagcac tagaaataag
     1441 tacatcaaat cacaatatgg cattaggctt gaagattaaa gaaactaaaa atggggggga
     1501 ttttgtcatt cagctttatg atcccaacca tacagcaact catttacgag cagagtttaa
     1561 caaatttaac ttagctaaaa taaaaaaact gactgtagat aattttcttg atgaaaaaca
     1621 tcagaaatgt tatggtctta tatccgacgg tatgtctata tttgtggaca gacatactcc
     1681 aacaagcatg tcctccataa tcagatggcc tgataattta cttcacccca aagttattta
     1741 tcacgcgatg cgtatgggat tgactgagct aatccaaaaa gtaacaagag tcgtacaact
     1801 atctgacctt tcagacaata cgttagaatt acttttggca gccaaaaatg acgatggttt
     1861 gtcaggattg cttttagctt tacaaaatgg gcattcagat acaatcttag catacggaga
     1921 actcttggaa acttctggac ttaaccttga taaaacggta gaactactaa ctgcggaagg
     1981 aatgggagga cgaatatcgg gtttatccca agcacttcaa aatgggcatg cagaaactat
     2041 caaaacatac ggaaggcttc tcaagaagag agcaataaat atcgaataca ataagctgaa
     2101 aaatttgctg accgcttatt attatgatga agtacacaga cagatacccg gactaatgtt
     2161 tgctcttcaa aatggacatg cagatgctat acgcgcatac ggtgagctca ttcttagccc
     2221 ccctctcctc aactcagagg atattgtaaa tttgctggcc tcaaggagat atgacaatgt
     2281 tcccggactt ctgttagcat tgaataatgg acaggctgat gcaatcttag cttatggtga
     2341 tatcttgaat gaggcaaaac ttaacttgga taaaaaagca gagctgttag aagcgaaaga
     2401 ttctaatggt ttatctggat tgtttgtagc cttgcataat ggatgtgtag aaacaattat
     2461 tgcttatggg aaaatacttc acactgcaga ccttactcca catcaggcat caaaattact
     2521 ggcagcagaa ggcccaaatg gggtatctgg attaattata gcttt
//
LOCUS       DAZOYF010000303         2541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZOYF010000303 DAZOYF010000000
VERSION     DAZOYF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.311"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..197
                     /locus_tag="ACHUOF_004258"
     CDS             <1..197
                     /locus_tag="ACHUOF_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR0613170.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="ACHUOF_004259"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="ACHUOF_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFR0613171.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="ACHUOF_004260"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="ACHUOF_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFR0613172.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..>2541
                     /locus_tag="ACHUOF_004261"
     CDS             1821..>2541
                     /locus_tag="ACHUOF_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFR0613173.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLW"
BASE COUNT          605 a          604 c          657 g          675 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg c
//
LOCUS       DAZOYF010000304         2523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZOYF010000304 DAZOYF010000000
VERSION     DAZOYF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.312"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            81..332
                     /locus_tag="ACHUOF_004262"
     CDS             81..332
                     /locus_tag="ACHUOF_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0613174.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            334..2430
                     /locus_tag="ACHUOF_004263"
     CDS             334..2430
                     /locus_tag="ACHUOF_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFR0613175.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT          695 a          589 c          702 g          537 t
ORIGIN      
        1 aatctttggt atcttaatct ttatccatac ggatatccga ccggatatcc gcgcgggtaa
       61 ttttgtaggt taaaggaaag atgagacaaa gagaagatag tgaccgcgat tggccttcgc
      121 tgaagattca gctggcgctc aaagataacg ggtggtcttc cttccgggag ctttcactcc
      181 atgctggtta tgctcaggat tcactgaaag cggtagttcg cacaccaaac aaaaaatacg
      241 aacgcgtaat ttctgacgct ataggtgtac cgccagaaat catctggccc agccggtatc
      301 gtaacgatgg atttatcagg aaggcgtcgt gatatgcagt cgtacgttac cgtaaatgac
      361 ctgctcggcg taccaggaat gccagccacc acaaaaggca tccgacaggc gttacagcgt
      421 ttttctcgtg atcttggtga cgttagtcgc cgtcgggaag gcacaaaagc cattgagtac
      481 cacattgatt gtctgcctga aataacacga aaggcactgc gtgaacgcta tgtggaacag
      541 ctggtggcta cagagaacaa tgtttctgaa gtgaaagctg ttaccagaaa aacacgcaat
      601 cctgacgctg tccaggcaat cgaagcatat cgcggttctc cacaactgat ggaagaacgc
      661 ctgaatgcgc tgaccgaaaa ccagcgctgg gtatctgaag caagagctgc gctggtggtg
      721 gaagtgctga agctggaaag cgccggtaac cccgggcgac tgaaagccat taactttctt
      781 gttgaaaaag cccgtaaagg tgagctgccg gagcgcctgc aacaggccgc agttaacgcc
      841 aatgcaaaac gtggcgctaa tcgtactatc agccgcgatc cactttacca gtgggttctg
      901 aaatacaacc agtcgcagaa cgccgccgaa cggctgctgt tactggcacc gggaaaacgc
      961 gacgaaataa aaccggaaga aatcagctgg ctgcctgaat ttctggcgca gtatcgccag
     1021 gtgaacggca gacccatgtc agaagcctac gaggattttg tcgcagaatg gcagcgtcgc
     1081 catgcggacg aaccgtacat gctggaggtg atgccttcct atgatgttgt ccggtatgcc
     1141 atgaaaaaac tgccggaagt ggtgaaacaa aaagggcgcg ttaccggcag tgaataccgt
     1201 cagcttgaag gattcacccg ccgcgactgg acagctatgc cggtgaatta tgtctggatt
     1261 ggtgacggtc acggtatgaa gctgaaatgt gcgcatccga tccacgggcg tccattctca
     1321 ccggaagtca cgtttgtgat cgatggtgga acacggtttg ttgttggctg gagtcttgac
     1381 cttgccgaaa acgtgttcgc cgttgccggt gcgatacagc acggcattcg caaccacgga
     1441 aaaccgttcc tgtattactc ggataacggt tccggtgaaa cggctgacat gctggacaaa
     1501 gaggtcgtgg gtattctgcc acgcctgggg attaaacacc ctaccggtat tgccggtaat
     1561 ccacagggac gcggcattat tgaacgcctg aaccggacac tgccgatgcg tatagcgagg
     1621 aggtatcgca cctattttgg caagggtgct gaccgggaat cgttgcgtgt acttaaccgt
     1681 gatctgcgtt cagcgtttaa cgccctgcaa caggacaagc ctctgaacga ccgccagaaa
     1741 gcggcaatgc gcgagctgcc atcgtgggcg gagcttattg aggctattcg tgaaggcgtg
     1801 gaatggtaca acaaccgccc gcattctgag ctgccgatga aaccgaacgg acggcattac
     1861 agcccgacag agttcagaaa aaaacgccag gcagaagaag acaccgaaat tgagtggctg
     1921 agtgatcttg agctgcgcga catgttccgc ccaatggtgg aacgaccggt aagacgctgc
     1981 gaaattcagt ggctgaacaa tatttactac gcgccggagt tgcgtgacga acacggtcgc
     2041 aaagtgctta tcagttatga cattcatgac gccgagcgaa ttacggtacg tcgcaaggac
     2101 ggcagcttta tctgtgaggc gatatggaac ggcaataaac gcgcagcctt tgctgtcagt
     2161 gctgaatatc acaaacaaca gcagcgtatt aaaggaatgc gcaaacgcgc tgaggaaaaa
     2221 attcgtgatg ctgaagatga aggtattcag attctggaac acaaacaggc tgaaccctgg
     2281 ctgagtaatg tttatcgccc ggttggtaat gtggtggcag ttcagcaacc ggaatatgaa
     2341 gaagagcatg acgaagaatt tgaacgcgat ttccggctgg ggatgcaaaa actttttgcc
     2401 atgcaggaag aagatgatcc gctggcctga ataaaaaaac agtccgcggg aacggactgt
     2461 taaataaagg aaacaaagct gatttgaaat aaccgaggaa atactatgac tgatattgat
     2521 gat
//
LOCUS       DAZOYF010000305         2520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZOYF010000305 DAZOYF010000000
VERSION     DAZOYF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.313"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            7..657
                     /locus_tag="ACHUOF_004264"
     CDS             7..657
                     /locus_tag="ACHUOF_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613176.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="ACHUOF_004265"
     CDS             629..1063
                     /locus_tag="ACHUOF_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0613177.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1836..>2520)
                     /locus_tag="ACHUOF_004266"
     CDS             complement(1836..>2520)
                     /locus_tag="ACHUOF_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR0613178.1"
                     /translation="GTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFMLSQTVL
                     NHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSITAIMSA
                     YQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICGNDFIVR
                     AGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKIGHWLSR
                     TVGGPFLIK"
BASE COUNT          760 a          475 c          528 g          757 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc taggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat
     1801 atctataaaa aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg
     1861 actgtcctcg atagccaatg gccaatttta tctgatacat atacacaaat acaggtatat
     1921 atgattgcgc agataggatt tggaatagtc acgctgaagc ctataattgc taaagccata
     1981 gggataaata atttggtgat atactccatt ccacatatgt taagagtatt tcttccagct
     2041 cttacaataa agtcatttcc acaaattatt tttgctatga aaatattaaa gattatcaag
     2101 cctaccgtgg tgataatagt attaatagct tgaatataac tacttgatat tatgtctgca
     2161 ttgaatattg agataatctt aaaaagtaaa ggtttctggt taagtagttg atacgcactc
     2221 attatagcgg taattgagaa gacaatgaaa ccaattttag attttccaaa aaacctgtca
     2281 cgtgttaatt ctaaaaacat acatctaccg agaggcaata accaccagta tgccatagcg
     2341 gagtctatgt tccaaaacca ttgaggatct agtaatgggt tatgattgag cactgtttgt
     2401 gacaacatga acgataacag tgatatgatt aaaatcacta tattgttctt gactatttct
     2461 ctaaagattg catcaattgc tattataaca aaaaggcagt ttataaacca tattgttcct
//
LOCUS       DAZOYF010000306         2507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZOYF010000306 DAZOYF010000000
VERSION     DAZOYF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.314"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..1204
                     /gene="yedE"
                     /locus_tag="ACHUOF_004267"
     CDS             <1..1204
                     /gene="yedE"
                     /locus_tag="ACHUOF_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR0613179.1"
                     /translation="SWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGEF
                     TRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVKL
                     RMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSWF
                     GARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQP
                     KLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYVQ
                     LGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGST
                     ILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRREA
                     PQTAKEIA"
     gene            1201..1434
                     /gene="yedF"
                     /locus_tag="ACHUOF_004268"
     CDS             1201..1434
                     /gene="yedF"
                     /locus_tag="ACHUOF_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR0613180.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            1541..2211
                     /locus_tag="ACHUOF_004269"
                     /pseudo
     CDS             1541..2211
                     /locus_tag="ACHUOF_004269"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(2251..>2507)
                     /locus_tag="ACHUOF_004270"
     CDS             complement(2251..>2507)
                     /locus_tag="ACHUOF_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR0613181.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT          526 a          656 c          682 g          643 t
ORIGIN      
        1 gtcatggcag caattcaaac acgcctggtt gattaaattc tgggcaccca tccctgcggt
       61 catcgcggcg ggtattctct ccacttacta ttttggcatt actggcacct tttgggctgt
      121 cacgggtgaa tttacccgtt ggggcggtca gctcctgcag ctgtttggcg tccatgctga
      181 agagtggggt tattttaaaa ttatccatct ggaaggatcg ccattaaccc ggatcgacgg
      241 catgatgatc ctcggtatgt ttggcggctg ctttgctgct gcgctgtggg ccaacaatgt
      301 caaactacgc atgccacgca gccgtatccg cattatgcag gccatcattg gcggcattat
      361 cgccggtttt ggcgcgcgtc tggcaatggg ctgtaacctg gcggcgttct ttaccggcat
      421 tcctcagttc tcgctgcatg cctggttctt tgccatcgcc actgccattg gttcatggtt
      481 tggcgcgcgc tttaccctgc tgcccatctt ccgtattccc gtgaaaatgc agaaagtttc
      541 tgccgcctca ccgctgacgc aaaaaccgga tcaggcgcgg cgtcgttttc gtctcgggat
      601 gctggtcttt ttcggcatgc tgggctgggc gctgctcacg gcgatgaacc aaccaaaact
      661 ggggctggca atgctgtttg gcgtcggctt tggtttactg attgaacgtg cgcaaatctg
      721 ctttacttcg gcgttccgcg atatgtggat caccggacgt acccatatgg cgaaagcaat
      781 cattatcggt atggcggtga gtgccatcgg gatcttcagt tacgtacagt taggcgttga
      841 acctaaaatc atgtgggcgg gaccaaatgc ggtaattggt ggtttacttt ttggttttgg
      901 catcgtgctg gcaggcggct gcgaaaccgg ctggatgtac cgcgcggtag aaggccaggt
      961 gcactactgg tgggtcggtc tgggcaacgt gatcggctca acgattctgg cgtattactg
     1021 ggatgatttc gctccggcgc tggccaccga ctgggacaaa atcaacctgc tgaaaacttt
     1081 tggtccgatg ggaggcctgc tggtgacata tttgctgctg tttgctgcgc tgatgttgat
     1141 tatcggctgg gaaaaacgct tcttccgccg tgaggcaccg cagactgcta aggagatcgc
     1201 atgaaaaata tcgttcctga ttaccgcctt gatatggtgg gtgaaccctg cccttatccg
     1261 gcagtcgcaa cccttgaggc gatgccgcaa ttaaaaaaag gtgagatcct ggaagtggtg
     1321 agcgactgtc cgcagtcgat caataatatt ccactggatg cgcgtaatca cggctacacg
     1381 gtgctggata ttcagcaaga cgggccgacc attcgttatt taattcagaa gtaatccctc
     1441 attcccgctg tggcccacag cgggaacatc gttacctctg acacatagat tttacccgcc
     1501 ccctctccta ttcttttcag tatgatctca ggaggttgct atgtgtggac gctttgccca
     1561 atcccaaacg cgtgaggatt accttgcgct tctcgcggaa gatattgaac gcgatattcc
     1621 ctacgatccc gaacccattg gcagatacaa tgtcgcgccg ggaaccaaag ttcttctgct
     1681 cagtgaacgt gatgaacacc ttcatctgga tccggttttc tggggatatg ctcccggatg
     1741 gtgggataaa ccgccgctga ttaacgcccg cgtagaaact gcctcaacca gtcgcatgtt
     1801 taaaccgctc tggcaacatg gtcgggcaat ctgttttgcc gatggctggt ttgagtggaa
     1861 aaaagaaggc gacaaaaaaa gccttatttt atctatcgcg ctgacggaca acctgttttt
     1921 atggccgcga taggtagcat accgtttgag cgtggtgacg aagccgaagg atttttgatc
     1981 gtcactgcgg cggcagatca aggtctggta gatattcatg accgccgccc gctggttctg
     2041 tcgccagaag ctgcacggga atggatgcgg caagacattg gtggtaaaga agcctcagaa
     2101 atcgctacca gaagctgtgt gccagcaaac cagttcacct ggcaccctgt gtcgcgcacg
     2161 gtgggtaatg tcaaaaacca gggcgcggag ttaattcaac ctgtttgttg acatcctcca
     2221 cgccctgaat gacgaggacc cctgctacgt tcaggctgtc gcctgaatca tttcggtggg
     2281 ttcctgcttc aacgagcggc ctgactgcac catctctcca caggcaagaa cggcgtgccc
     2341 cgccgctaaa atgttacgag ctccatttac atcggcgttc gctgtatatc cacatacctg
     2401 gcatctgaat ttactttgtg acaggcgatt ttctttcgct gtatgaccac agcacgcgca
     2461 acgctggctt gtgtacgctg gcggaacagc aagcacctga ccgccac
//
LOCUS       DAZOYF010000307         2491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZOYF010000307 DAZOYF010000000
VERSION     DAZOYF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.315"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>2491
                     /locus_tag="ACHUOF_004271"
     CDS             <1..>2491
                     /locus_tag="ACHUOF_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0613182.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQEQ"
BASE COUNT          730 a          578 c          602 g          581 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggagcaa t
//
LOCUS       DAZOYF010000308         2463 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZOYF010000308 DAZOYF010000000
VERSION     DAZOYF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.316"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(746..2350)
                     /locus_tag="ACHUOF_004272"
     CDS             complement(746..2350)
                     /locus_tag="ACHUOF_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0613183.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          648 a          575 c          526 g          714 t
ORIGIN      
        1 aagacctata ggtttggtga ttgattatta taaatatcat caccccttaa ggtattccct
       61 tagagttaat ctttaattgg tctttaatta aatcttttat ttaatactta aagttaatct
      121 ctaattgtgt ctttaagtta actttaagta atactataag ttaatcttta attgtgtctt
      181 taagttaact ttaagttaag cccctgtagg gactagagac cataattaag atgattgtgt
      241 cttattgcta ttcacttatc gactgaccca gagcacacag gaagcattca ctttaagtag
      301 aacgcttggt gtctgtaatt ggtcatcccg cccctgaaag gatgcgataa gttacggtta
      361 gttgtcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      421 tccttgacaa gtagagaaaa ccatgagtca aaaacatgac cacacaggtc tgtagtaagt
      481 gtcatcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      541 ccccttgaca agcagagaaa actatgagtc aaaaacatga ccgcacaggt ctgtagtaag
      601 tgtcatccta gaagagaaaa ctttttgtgt cccttctccc tatagtgagt cgtattaatt
      661 atcccttgac aagtagagaa aactatgagt caaaaacatg accacacagg tctgtagtaa
      721 gtgtcatcct agactatggt ctcctctagg tctccttata aacatcagcc atcacattga
      781 ccagcctatc aacctcgacc tctttagcat aacggtcgta cgtaatagac cctgaactat
      841 gccccatgat cgcctgtgca tacgccaact cgaccccatg ttgttttaac tgggccgcaa
      901 ctgtatgtct aaacgagtga aacgctaagc cgctacggtc tacgaggact ttaggcaaca
      961 acgtccgatt aaaccattta ctcacctgct caccgtaacc gttcttcatg agacgcagac
     1021 catcgaacac catagcgtca tcaccgtcag cgcctctacg ggtctctaca agcgccataa
     1081 agtcagccaa tataaaacca taggccccat cgaccagagg cacacagcgg tcactatgag
     1141 cgttcttgat gctcttaccg ggtagactac tatcgtcttc attgatatga atataaacgg
     1201 ttcccgcctc agtggtcctg acgtctttga cctgtagttg ggctatttca ttgagtctcg
     1261 ccccagtgat agcagccaag gcggtaacat aataatgata cggcttacca gaggtcttct
     1321 gagagtatgc cttagctgcg accagtagtt gccccacctg ctccgtagag aaggcgttac
     1381 gggcccctga ggctttacgc tgagggactt taagctccag accctcagtc atgttctttt
     1441 taatcaagtc attgcgcaca gcccacctaa agacagcagc catcttaata agatacttgt
     1501 tgttgatggt aacgacatcc agacaatctg tcttatcttc acggctcaac aggtcagcaa
     1561 gtgggacatc cttgaagcgc tgcttacggt tcctaggtaa ctgctggagc acatcacgga
     1621 ccctaagcat gtcagccctg ttagcgtctg cattgagacc cagatggtcg aatatttcga
     1681 ttaacgcagc gtgtgacgcc ttgttttccc ttaaggttgc aggcttccag ttctgatagt
     1741 tctccgcttc gtattgttca aagaggctcg ctatggtaat gctaggcgta acttctggtt
     1801 ttacttctgg agcattaaca gatagggaca cagagtctgc ttggttatct tgcctatcaa
     1861 tatcattaaa aagatcgacg taatctatca gaggctgact gtctcccgct tcgatacgcc
     1921 tcatacaggc ttttagcacc ttcagggcct cattgatgta acgatgctgg tcaatgctta
     1981 gcggctcact agccagagca tcggtaaggc tctcaccaag ttctccgtat tggtcccggt
     2041 agatatccct catgtcaggc tcaaagagat ctgaacggcc catagacagt tcacactcag
     2101 cgatgtcctt aaggtgttcc ctcatttcct cataggtagc gttgggtctg tctgcgtgta
     2161 ttgccttgag tgctgccttt atgtgccttg agtaagccat agcggtgcgt ctatccttgg
     2221 tccgtaatga tattgaggtc attctatcat tgtttgattg tctgacccta agacggaagt
     2281 agtagacgcc gttacgctgg tagagatagt agactgctgg aagtttgtga ctactggata
     2341 gctgaatcat atgtctgcat gccctgagag ggttacacag tgggttacag taagtaactc
     2401 tgcaaggagg cttaaagccg tatcccttag gattcagtag gttggagtac tttaagaaag
     2461 gtc
//
LOCUS       DAZOYF010000309         2441 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZOYF010000309 DAZOYF010000000
VERSION     DAZOYF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.317"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..567
                     /locus_tag="ACHUOF_004273"
     CDS             <1..567
                     /locus_tag="ACHUOF_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0613184.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            525..1202
                     /gene="bglJ"
                     /locus_tag="ACHUOF_004274"
     CDS             525..1202
                     /gene="bglJ"
                     /locus_tag="ACHUOF_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR0613185.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="ACHUOF_004275"
     CDS             complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="ACHUOF_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR0613186.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2169..2405
                     /locus_tag="ACHUOF_004276"
     CDS             2169..2405
                     /locus_tag="ACHUOF_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR0613187.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          628 a          534 c          627 g          652 t
ORIGIN      
        1 ttagtcattg ctgatttagc cggtgaaagt gaagatccac gttctgtttg tgaacattat
       61 tattctttaa tctcacaata tcgggaaatt cactgggttt tcatggtgtc acgctcctgg
      121 tattcccagg ctgtagaact gctcatgtgc cctacggcga cgttattgtc tgatgttgaa
      181 cccattgaga atctggtcaa gaccgtacgt tccggcaata cgcacgcaga gcgtatcagc
      241 gccatgctga cctctccggc aatgactgaa actcatgatt ttagctatcg ctccgtcatt
      301 ctcactcttt cagagcgcaa ggtactgcgg ctattaggta aaggatgggg catcaaccag
      361 atagcttcat tgcttaagaa aagtaataaa actatcagcg cccaaaaaaa cagtgcgatg
      421 cgtcgactgg caattcacag caacgctgaa atgtatgcat ggataaatag cgcgcagggt
      481 gcaagagaac ttaacttgcc ttctgtttat ggagatgccg cagaatggaa cacagccgaa
      541 ttaagaagag aaatgtcgca ctcatagaaa aatgcgtcat gagtagtatc ggtattgaga
      601 gtttattcag aaagtttgcg ggcaaccctt ataagctcca tacctatacc agtcaggagt
      661 catttcagga tgccatgtcg cggatctcgt ttgcggcggt cattttttct ttttctgcca
      721 tgagaagtga gcgcagagag ggattatctt gcctgactga gctggcgatt aagtttccgc
      781 gtacccggcg tttagttatt gtggatgatg atattgaagc tcggctgatt ggttcattgt
      841 cgccatcacc gctggacggt gtattaagta aagcgtcaac gctggagatt tttcatcagg
      901 aactcttttt gtcattaaat ggtgtacgtc aggcgaccga ccgactgaac aatcagtggt
      961 acattaacca aagccggacg ttaagcccga cggagagaga aatattgcgc tttatgtcgc
     1021 gtggctactc aatgacacaa attgccgagc agcttaaacg taatatcaaa acgatccgag
     1081 cgcataaatt taatgtgatg tcgaaactgg gcgtcagttc tgatgcaggg ttgttggagg
     1141 ccgaagatat tctgttatgt atgcggcatt gcgaagcaag taatgtgttg catccctatt
     1201 aatccgcatg atgccgggtt tacttccccg gcagtgcttt catttcagcg tacaatagcc
     1261 acattgctgc acatccggta agcgataacg ctggcagcaa gtgcggcgca ccagcaggcc
     1321 gtcgcgcagt accacggtac gccagagtgg attatcttca ccgttcgtga gcgttttctc
     1381 aaaaaagagg gcatggcgca gcgattcaac agtagcctcg ccgagcagtt gcttcatctc
     1441 agtgagatac cagttgatca aataaccggt attactccag ataagtttgc cgttgatctc
     1501 tccggtcgct tctagtgctt gcacaaccgg aaccagcgcc tggctgatta acgtttccat
     1561 tcgctgctgc ggcgaatgtg gtgttgcgtt tttatcttca cacacatcga cccagaaaca
     1621 ggcgacgcgt ccggtttcgt gaaactcagc atggaaatgt tccggcgaca catctaatgc
     1681 tttttcctgc gtcagtagcg ccagcattaa tggtggcacc atcaggccga tataccattg
     1741 tgcccatagt gagatcagcg gtttgttctc gcggatcatc gttggttggt tgcgatagat
     1801 atgatcggaa tagaccgcca gtagagaact tagcgcattc ggtgatgacc attgcgccag
     1861 cgtcatggcg ttaagcggag cgggttcatc caggcggata aactccagca aatgttcacg
     1921 atgtttggcg atcatcgccc gtacggcttg tgcaagccca gcatcctgcg gctggagatg
     1981 cgttcgccag atgacatctt catagagcgg tgcggaacga taggccataa tcgtgatagt
     2041 aatctaaatg ataatgattg ctaatcctag cgataggttt actcgatagc aagggatttc
     2101 tctggcttgc aaatgataaa aattatcata tgatattggt tttcattatc aatgaaagag
     2161 atgaaaccat gttgcaacgt acgctgggca gtggctgggg agtgttgctg ccgggattgc
     2221 tgattgcagg gctgatgtat gcggatttat cgccggatca gtggcggatt gtcattctga
     2281 tgggattagt attgacgccg gtgatgctgt atcacaaaca gttgcggcat tacattttgc
     2341 taccatcgtg cctggcgctt attgctggca tcatgctgat gataatgaat ttgaatcagg
     2401 gatgacaaaa cagggaagaa acaagaaagg aagtaaagat a
//
LOCUS       DAZOYF010000310         2436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZOYF010000310 DAZOYF010000000
VERSION     DAZOYF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.318"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..360
                     /locus_tag="ACHUOF_004277"
     CDS             <1..360
                     /locus_tag="ACHUOF_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR0613188.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="ACHUOF_004278"
     CDS             361..726
                     /locus_tag="ACHUOF_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0613189.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1411
                     /locus_tag="ACHUOF_004279"
                     /pseudo
     CDS             723..1411
                     /locus_tag="ACHUOF_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
     gene            1605..1680
                     /locus_tag="ACHUOF_004280"
     tRNA            1605..1680
                     /locus_tag="ACHUOF_004280"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="ACHUOF_004281"
     tRNA            1682..1758
                     /locus_tag="ACHUOF_004281"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
     gene            1761..1836
                     /locus_tag="ACHUOF_004282"
     tRNA            1761..1836
                     /locus_tag="ACHUOF_004282"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Thr,seq:tgt)
     gene            1840..1914
                     /locus_tag="ACHUOF_004283"
     tRNA            1840..1914
                     /locus_tag="ACHUOF_004283"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1873..1875,aa:Gly,seq:tcc)
     gene            2207..2422
                     /locus_tag="ACHUOF_004284"
     CDS             2207..2422
                     /locus_tag="ACHUOF_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR0613190.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          640 a          520 c          653 g          623 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtaggt agagcaactg acttgtaatc
     1801 agtaggtcac cagttcgatt ccggtagtcg gcaccatatg cgggcatcgt ataatggcta
     1861 ttacctcagc cttccaagct gatgatgcgg gttcgattcc cgctgcccgc tccagtcaga
     1921 gtctttcagt ctgcgatgat gggaaatccc ggagtgactg aaagacgttt aagttatgaa
     1981 tgatcgcttt tttttgcaaa attgctgtgc agaaatacta accttcgggc aggcgatcat
     2041 tcataagcac tctgctttta ttccgattaa ctgtgagtgg tttgttggat agagtgcttt
     2101 ccttattgta tatatcgttt cgcccgcttt tgcggttttt tcttttcaaa tccctttcat
     2161 ttctcagtgt aaaactacgc catccgttat ttgcggaggt gaggctatga aatccatgga
     2221 caaaatttca acgggcattg cctacggcac ctccgcaggc agtgctggct actggttttt
     2281 acagtggctt gatcaggtca gtccgtcaca gtgggctgcg attggtgtac tggggagtct
     2341 ggttctgggc ttcctgactt atctgacaaa tctgtacttc aaaatcagag aagacaagcg
     2401 taaggctgcg agaggtgaat aatgtcgcca tcatta
//
LOCUS       DAZOYF010000311         2412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZOYF010000311 DAZOYF010000000
VERSION     DAZOYF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.319"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..545)
                     /gene="ygeQ"
                     /locus_tag="ACHUOF_004285"
     CDS             complement(<1..545)
                     /gene="ygeQ"
                     /locus_tag="ACHUOF_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
                     /protein_id="HFR0613191.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..2196)
                     /locus_tag="ACHUOF_004286"
     CDS             complement(1207..2196)
                     /locus_tag="ACHUOF_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613192.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2280..>2412)
                     /locus_tag="ACHUOF_004287"
     CDS             complement(2280..>2412)
                     /locus_tag="ACHUOF_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613193.1"
                     /translation="REQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT          645 a          553 c          577 g          637 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagcctacg acaaatcgaa gacaaccagc taacctattg tttcttaaag acaggtgaat
      841 ccagttggtt gtagtgatag tcacacattt agagatagaa ggtcaagact tgaagcacgt
      901 aatattccct ctaaaggaat agaactcacg gtctgactac ccggcccttt tggtctggct
      961 caggtcaggc tatgggggct tttggctcgc tctttatatt agatgaggtc tcacattttt
     1021 cagtcaaaac tcatcgtcta atagcccata gccagaccac cagtcccagc tttagcagga
     1081 agaccacgag atagactttg tgtaggtcca tttggccctc ctactttctg tattcacaaa
     1141 ctagattact agtacaaaat aaaggccaga catccagttc ggaagtctga ccaatagtcg
     1201 ttattgttag ttagcttctt tagtgaagct caggcgtttg actacaggca ggacagcagt
     1261 agccgagcca gtacccatct tgatggtcgt agcagcttca gctacaacaa acaccttgcc
     1321 gagaatctca aggtcgtgag actgggagtc tccatcacgt gaaaggacca cagtaatgct
     1381 ctcgccatcg tgacggaagc aaccgaagct cagagccgta aaacgcttag ccatcatgaa
     1441 cacgccacgg tcttcaccgt tgtaataggc gacgtcagtc cccagcatat cgacgatagc
     1501 gtcttcacca ccgaagccct gcttagcagc cagacgggag agagccttga ggtgtgactc
     1561 atgggcgata atgccgtagt cagaaacctc agaaccgaaa gaagactccg tgttctcgta
     1621 cagggcatcc atgatagcgt cagccttagc ggtcaggcta ccagtcaggg ccggaagttc
     1681 tacggtcttc agcagctttg aggactccag cacgtcaacc atcttagagt taatggtacg
     1741 aatcacgtta tcgcagatgt tcagtgcgtc ctgctggagc gtcccctgag tgaaaccagt
     1801 ggtcgacttc tggtcccaaa gctttgtttg gtgagacacg aggatcgcct gttcaatcgg
     1861 ctcaacggtg atttcagtgc cagacggagc ggtcacaact tcaggtcgct taccaccacg
     1921 gacagggacc agcacagagc ggtcagccag agtgtgagca atgattggag tcggtaagct
     1981 gttacgggtc ggcaggactt ctacagggaa cgggatgtta tcttgagcgt ctttaagtac
     2041 accgttcagc atagccttaa cagtcttagc taactggagg gcatagacct gagggtcggc
     2101 gttagtcggc agaccttcat tgacctgaat gttgtcggta tcggtgatgc gtactggctc
     2161 aatgccaaac tggaaagcgt ttttagtcat attcattata attaatctcc ttgagtcaaa
     2221 tacttgaagt ccctaacagg cagtcaggga ccgtgtgttc tcagatgagg gacgcttgct
     2281 tacaagctgt ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgtgag
     2341 cgaccagcgg aatgagtagc gctgcggagg ttttcaccat aggtccggcg ttcagctttg
     2401 acctgttcgc gg
//
LOCUS       DAZOYF010000312         2410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZOYF010000312 DAZOYF010000000
VERSION     DAZOYF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.320"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..108
                     /locus_tag="ACHUOF_004288"
                     /pseudo
     CDS             1..108
                     /locus_tag="ACHUOF_004288"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap.; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
     gene            60..233
                     /locus_tag="ACHUOF_004289"
     CDS             60..233
                     /locus_tag="ACHUOF_004289"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFR0613194.1"
                     /translation="MPSSAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALR
                     MLQEYIRIQCLGG"
     gene            complement(322..834)
                     /locus_tag="ACHUOF_004290"
     CDS             complement(322..834)
                     /locus_tag="ACHUOF_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR0613195.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1029..1793
                     /locus_tag="ACHUOF_004291"
     CDS             1029..1793
                     /locus_tag="ACHUOF_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613196.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1744..>2410
                     /locus_tag="ACHUOF_004292"
     CDS             1744..>2410
                     /locus_tag="ACHUOF_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR0613197.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          679 a          538 c          611 g          582 t
ORIGIN      
        1 atgctcttcg gcgcaagctt gataatggtg gtcgggtgct cgtcaaagga aaatgccctg
       61 tgccatcctc agccgaaacc tccagcgcct ccggcatggg caatgatgcc accgtcgaac
      121 tctctccagt tgctggacga aacgttctcg gtatccggga cggaatcatc agcgaccaga
      181 cagcattgag aatgcttcag gagtacatca ggatccaatg ccttgggggg tagcggtaat
      241 tttactcatt atccttcaaa tcagattctg ttgtcagagg aatgggggaa gctggattcg
      301 caagagcttt ttatgggcag tttagctaaa aaaaggcaac acgaagctaa aactaccaac
      361 ataaagtgct gaccagctag cagctgaaat cagactcact gtatacactt tcactggatg
      421 aagttgcaac attcctgcca ccaaaggagc aatgtaacgc agcacggcga taaagcgcga
      481 agtgaaaagt attgacacag agttattttg caactgaagg cgaacccgtt ctattgtatt
      541 ggaacggctc gatattatac atgcaagttg agggatatgc ccaattatta taccaagatg
      601 gtaattaacg atagtaccgc accatgcgcc actcatgacc gtgattccag cttcccatgg
      661 tgaaagagtc gtcttgctga ctgtaattac agtggccatc ataattgatg ctggtggcaa
      721 tatcgccgaa atgagtaaag tggattttgt gaacgctatt ataaaaagaa gcccccatag
      781 gcgatatgga tgaagtacaa aatagttcat caaagcattt atccactcca tcaatgtctt
      841 tccttctgtg aatttttctt tgctatttaa ccgtgatacg acttaattta ggcttaaaca
      901 ccttacattt acaaggtact cctgtggggg gccctgccac ggagcgtcgc aggagtgagt
      961 gatgaggaag aattaaagca acctattgtg acaggcagcg ataacagtaa atcacaggaa
     1021 gcataatcat ggcaaaaccg gactgggagg ccatcgaatc ggcataccgg gccggagtcc
     1081 ttagcctccg tgatataggc gagaaatacg gcgttacaga aggggctatc aggaagaggg
     1141 ccaaaaagct tgattgggca cgcagtggcg gtacgcaggt ttgcaaaaat ggtacgcaaa
     1201 aaaggaaagt gcgtaccagc agaaagcctg ccattactgg ccttacacaa aaaagtacgc
     1261 aactaaaaac agaatctaca ccggatacga aaccgatacg cggaatgcgt accgatcccc
     1321 cgactaaccc attccaaccc ggtaaccagc aggcattaaa acacggtggt tatgcccgtc
     1381 gccttctgct caaagatgag gtgatagagg acgctaaagc gttgacgctc gaggacgaat
     1441 tatttcgcct tcgtgctaac aaccttgtcg ctgcagagaa tattggtcgg tggttggtgt
     1501 cgctggaaga tgctaatggg gaccaggaaa ggaagatgct gattgaaaat atcagcgccg
     1561 ccgagaaagc aatgatgcgc aatacagttc gtattgagtc catcgttggc acgcttgcga
     1621 cggtaggaaa aatatttgct gatacagcct accgcaaggc cgccactgat aaggtgtctc
     1681 tggaggctga tcgtcttcgc cgtgatgcag gtattgatga tggcaatgga gagcgtgacc
     1741 tcaatgactt ctactctgac atccaaaccg acgctgaatc cggctttacg tagtttctgg
     1801 actatgcggg cacgtaacaa agtgctttat ggtggtcggt catcgtcaaa atcatgggat
     1861 gccgctggca ttgccatatt tctgtcgaat aaatacaccc tgcgtttttg ttgtgcccgt
     1921 cagatccaga ataaaatcga agagtcggtg tataccctgc tcaaaattca gatagacagg
     1981 tttggtctgc ggcaccgttt ccgtattctg aacaacaaaa tcattaaccg ggttactggc
     2041 tcggaatttg ttttttatgg attatggcgc aacatcgaag aaattaagtc actggagggg
     2101 atcgatatgt tgtggttgga agaagcccac gcactgacgg aataccagtg gaaaattctg
     2161 gagccaacga tccgtaaaga gggttcggaa tgctggttca tattcaaccc cggacttgtt
     2221 actgatttcg tctggcgcaa cttcgttgtt gatccgcccg aaggcactct catccgcaaa
     2281 attaactatg acgaaaatcc gtttctgtct gacaccatgc ttaaggttat cgacgcggcg
     2341 cgacgccgtg atccggatgg ttttaaacat gtgtatgagg gcgttccgga gtctgatgat
     2401 gatgcggcaa
//
LOCUS       DAZOYF010000313         2381 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZOYF010000313 DAZOYF010000000
VERSION     DAZOYF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.321"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACHUOF_004293"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACHUOF_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR0613198.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1861)
                     /gene="phoA"
                     /locus_tag="ACHUOF_004294"
     CDS             complement(446..1861)
                     /gene="phoA"
                     /locus_tag="ACHUOF_004294"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR0613199.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="ACHUOF_004295"
     CDS             complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="ACHUOF_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR0613200.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
BASE COUNT          539 a          613 c          554 g          675 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca tcggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg
     1741 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
     1801 cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
     1861 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
     1921 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc
     1981 aataacttct ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg
     2041 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
     2101 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
     2161 tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc
     2221 atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata
     2281 gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg
     2341 attgccagcg aaacgtttct cttatttaaa taattaccca c
//
LOCUS       DAZOYF010000314         2350 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZOYF010000314 DAZOYF010000000
VERSION     DAZOYF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.322"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..417)
                     /locus_tag="ACHUOF_004296"
     CDS             complement(<1..417)
                     /locus_tag="ACHUOF_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR0613201.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQ"
     gene            complement(427..1119)
                     /locus_tag="ACHUOF_004297"
     CDS             complement(427..1119)
                     /locus_tag="ACHUOF_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR0613202.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            complement(1122..1577)
                     /locus_tag="ACHUOF_004298"
     CDS             complement(1122..1577)
                     /locus_tag="ACHUOF_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFR0613203.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(1577..>2350)
                     /locus_tag="ACHUOF_004299"
     CDS             complement(1577..>2350)
                     /locus_tag="ACHUOF_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFR0613204.1"
                     /translation="IFSQSYLLYVIAQGTDVGNVANKANEAGQGAYDAQVRNDEQDVI
                     LADHEQRISAAEATLVNHEERIRQAESTLQDHETRIAQNESDISSLDTRVQSLESQVS
                     DHETRIDALEYATTRKKSEVVYSGVSVTIPTAPTNLVSLLKTLTPSSGSLAPFFDTVN
                     NKMVVFNENKTLLFKLSIVGTWPSGTANRSMQLTFSGSVPDTLVSSRNAATTTDNILL
                     ATFFSVDKDGFLATNGSTLTIQSNGAAFTATTIKIIAEQ"
BASE COUNT          536 a          617 c          577 g          620 t
ORIGIN      
        1 ttgcattgct tctggagacg atgacgcaag gcgtgcgcca gccgctaagg tgccgataga
       61 attacgctgc tcttcatcaa tgaatcccat gcctttacga acggattcaa tctggtctgg
      121 atattgagta gccaactgac gcaaagcacc gcgatcacca gacgcataag cattagcgta
      181 cgcctgctga aattctttct gccgctgagc ctgcttttcc tgctgaaaaa cacccgcaat
      241 acctgaaaga ccttgcaaag cagtcagccc aacattgtta gcgcctgaac gctcaatatc
      301 attgttctgc ctgataagct gaagcgtatt gccgatgtca tttacgctcg gagcgtttga
      361 gttgacgccg ccgataccag ccaacaatcc gccgtttgtt ccttgccaag tagccatgat
      421 tcccccttaa aacaacgagc caagcaatcc gataccagca ccaataccag cgccccaagg
      481 cgttgatgtt cccaaaaggc tggcaagacc tgcaccggca atcgcaccag acgtgccacc
      541 gctaattgca gtctgaagac ttgatggttt attggcatta gcagcggcaa gagctgcgct
      601 ttgctgtgca atgctgctca tgttgttggc gtacgtctgc ccggcgtttg cctgaccttg
      661 cagcgcacca agcccaacgt ttgccagatt gttgtaattg ctcatctgat ttgataacca
      721 agactgaccg agtgtcggcg cgatcgtagc cagttgattg cttgtggctg tcgaaccaag
      781 tccacccgtc gcctccgcag cagcaagact ctggtaacgc gcctgccctg caaggtcttt
      841 atactgctta gagttgtaat actgattaag tgcctgcccc tgaccttcta aactggaaag
      901 gttctgaagc tggttaacat actgctccgc aagaggcgtg aacggagcaa ggtttttcat
      961 gatcgtctgc cactgctgat tttgcaggtc tgcagcatac ttctgggctt ctgctgcata
     1021 ctttgcgctt ttatcagaac tgccaccttt cccgcctttt tcagggcaat aaggttcctc
     1081 gccgcgcagt tttctgccca gcttaaatgc atataacatg gctatctccc gtgattcagg
     1141 aagtcgatta gttcttcgcg tgtggcgctg taaaaagtca cgtcatccac gcctttgaag
     1201 tatttcttga tggttcctac acgcttaagg ccaatcattg cgcagtacat ctgaccgtgt
     1261 cggaatttgc gtgcagcgaa cgatgttacg cactgaacgg tggtgttagt cagaatgtat
     1321 cgccagaacg ccagcccaat ttccttgctg aagccgcgaa tctctggcag gtacatggcg
     1381 tggcaatcta atgtcagcgg ctgaatctcc tgatagtaaa caatgccgcc aaactgaccg
     1441 tgcacgttca cctcaaagta acggcattca ggtttgtagt cgtatccatc accgttgttg
     1501 ctcccggcga taatgtcagg gtgatttccg actgcttcta tcaggtcgat gtttcgcgtt
     1561 ggtttgaatg taatcatcac tgctccgcga ttatcttgat ggttgtggca gtaaacgccg
     1621 caccattcga ctgaatggtt aacgtactgc catttgtggc aagaaagccg tctttatcca
     1681 cgctgaagaa cgtagctaac aggatgttgt cggttgttgt cgccgcatta cgactgctga
     1741 ccagtgtgtc aggaacagag ccggaaaagg ttagctgcat tgacctgttg gcggttccgc
     1801 tgggccacgt gccgacaatc gacaacttga agagcagggt tttgttctcg ttgaacacaa
     1861 ccatcttgtt gttaacggtg tcgaagaatg gtgccaacga gccggatgac ggtgtgagcg
     1921 ttttcagcag gctaacaagg ttggtcggcg ctgtcgggat ggttacagat acaccagagt
     1981 aaacaacctc tgacttcttg cgtgtggttg catactccag agcatcgatg cgcgtttcat
     2041 ggtctgaaac ctgcgactcc agcgactgaa ctcttgtgtc aagcgacgaa atatcgcttt
     2101 cattctgagc tattcgtgtt tcatggtcct gaagagttga ttctgcctgt ctgattcgct
     2161 cctcatgatt aacaagcgtt gcttccgcag cagaaattcg ctgctcatgg tcagcgagaa
     2221 tcacatcctg ctcatcgttc ctgacctgtg catcataagc gccctgtccg gcctcgttgg
     2281 ctttgttagc cacgttacca acatcagtgc cctgtgcgat aacgtaaagc agatacgact
     2341 gcgagaagat
//
LOCUS       DAZOYF010000315         2345 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZOYF010000315 DAZOYF010000000
VERSION     DAZOYF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.323"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..797)
                     /locus_tag="ACHUOF_004300"
     CDS             complement(<1..797)
                     /locus_tag="ACHUOF_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0613205.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLT"
     gene            complement(1396..1644)
                     /locus_tag="ACHUOF_004301"
     CDS             complement(1396..1644)
                     /locus_tag="ACHUOF_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0613206.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1907..2002)
                     /locus_tag="ACHUOF_004302"
                     /pseudo
     CDS             complement(1907..2002)
                     /locus_tag="ACHUOF_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1999..>2345)
                     /locus_tag="ACHUOF_004303"
     CDS             complement(1999..>2345)
                     /locus_tag="ACHUOF_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0613207.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          607 a          431 c          562 g          745 t
ORIGIN      
        1 gttaatcttt gcagggattg cagagcatgg gatgacaatg ggttatcact aatatctatt
       61 gaacattcat tcctcagatt aagaatgctt tccgggatat gacttatctg atttatgtca
      121 aaataaccgt tccttaaatt ttccggcagg tatggaatat cggttaacat attgtcgatt
      181 gcccatatgg agtgaatgtt gtgaggtaga ggggggagtt cactaagaag attccctatt
      241 gcgctaatct cttgtaaaga aaatggtaaa ggtggtaaaa ctgccagccc attacatgat
      301 acatctaatg tttccagtag ttctggtaaa acaggaagag aacataattg attatttgag
      361 acatgaagct ccttcaaaga tatggggagt gtgggtagtg tgattagttg attgtgggac
      421 gcattcaata atttaagtcc ttgaggcaaa gcaggcagtt caataagtct gttatagctg
      481 acattaagtt gtgtaaggga cgcaggcaat ggggagatta agcttaaatt atttttactt
      541 atattaattg atttaattcc cggggggatt tcaggtaatg ttgtcaggcc taattcagac
      601 aagtccaggt tcgtctcttg gttttgtaga catgatacta gtcgctgaaa agcgatgtct
      661 cgttgttctt cttgtatgcg gttatttttc cattcagtcc aatgggttag ataattttca
      721 tatgcgcggc tagtgtcgat tgaataagtg gagaacgaat ttgaaattaa tctgtgattg
      781 ttatttgtcg ggagcataaa tatcagggtg tattctttat gttgagaggt gttggattct
      841 tttttatctg ctttgcaatg ttattggttc ccttcagcag tagacaggaa cttctggagg
      901 aggcggatat atagtttgtg taagcatatt aactccatat gttatatatt gaagaacttc
      961 tgctttacac tatcctaaac tggatcggtt taatgtaaaa aaacgatact ttttgggggg
     1021 gggcaatttt tccttaaggt atgaaaaaag gagctgaagc tatataatag ctattaatgc
     1081 cactggtatc agtgaatcat gtatataaaa aactgtgtta aggaggctgt ccattagcgg
     1141 ttttttattc ttctggaacc gcggcatctt tgcgtatctt tttgtcttct caccgtctgg
     1201 tcggtgtttt gctgagactg ctaatttcct gtctttgcta gtgctgtact ggtactgccc
     1261 aatcatgatt ggcgggggcg ggcggagttg agaccgcaga cacgtcgtat gcaagaacgt
     1321 gctgcggctg gctggtgaac ttccgatagt gcgagtattg aatgatttcc agccgttact
     1381 gattttacgt gctaattagt gaacaaacca ctcgtcagca gactcccagg tatctttcag
     1441 agtttcctga acaaaagttt tagctgaatc tttatcggcg gtgcgcgtaa cagaaaggcc
     1501 atcgttgctg gtggctttta cgatcacctc tacatcgtca taacgtttac tgatgcgtcg
     1561 ggttaattct tcctttaacg catccacagc accggttggc attttagtca ttttctcttt
     1621 ggctatgcag atttcaaaac gcataaaaat ccctctgcac tgtgttttta tacagtatta
     1681 tttttaactg tatggataaa cagtgtcaag agtgagggaa agtgattttt tggggcatgc
     1741 atggggcaaa aatatttgtt gtggggcatt ttggggcatg attgggacac tttgttacat
     1801 atgaactttg ccgattttca tatgacatgg caatccaacg ctttgattat tctaaagaaa
     1861 acacatgctc ttgggcgttc tttagtgatt tttaaaaatc ccgtatcacg cagttaaagt
     1921 ggcgggcata ctcttcaagg ctggtgatcc ccaaccgcac ccatttagga tgtgaccatt
     1981 gcggtagacc gatgtaaatc ataacgcgtg aaggatctct tccacgctac gcacacgggc
     2041 gatgcgcggg tagatatgat taatgctgtt attgtgttgc tcggcgctgg cggcactaca
     2101 ggcgtcttcg gcaatcacca gattaaagcc aagttcccag gcattgcggg cggtggattc
     2161 aacaccgata ttggtcgaga tcccacataa cactattgta tcgatacctc ggcggcgcag
     2221 ttgcaactcc agatccgtac cgtaaaacgc accccactga cgtttgatga tttcgatatc
     2281 actgtcagtt gcacctaatg cagctggatg ttgccaccag ttttcgggca acactttagc
     2341 ggggg
//
LOCUS       DAZOYF010000316         2342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZOYF010000316 DAZOYF010000000
VERSION     DAZOYF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.324"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            22..531
                     /gene="eco"
                     /locus_tag="ACHUOF_004304"
     CDS             22..531
                     /gene="eco"
                     /locus_tag="ACHUOF_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR0613208.1"
                     /translation="MKKESKKMKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
     gene            complement(653..2277)
                     /gene="mqo"
                     /locus_tag="ACHUOF_004305"
                     /pseudo
     CDS             complement(653..2277)
                     /gene="mqo"
                     /locus_tag="ACHUOF_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
BASE COUNT          550 a          644 c          584 g          564 t
ORIGIN      
        1 atcatcggtg aaaacctata aatgaagaag gaaagcaaaa aaatgaagac cattctacct
       61 gaagtattgt ttgccgcttt cgctaccact tccgcctggg cggcagaaag cgtccagcca
      121 ctggaaaaaa tcgcgcctta tccacaagct gaaacaggca tgaagcgtca ggtgattcag
      181 ttaaccccgc aagaagatga atctaccctg aaagtagaac tgttaatcgg tcagacgctg
      241 gaagtcgatt gcaatttgca tcgtctcggc gggaagctgg aaagcaaaac gctggaaggc
      301 tggggctatg attattatgt ctttgataaa gttagctcgc cggtttccac gatgatggcc
      361 tgcccggatg gcaagaaaga gaagaaattt gtcaccgcgt atctgggcga tgctggaatg
      421 ctgcgttaca acagcaagct gccgatcgtg gtgtatacgc cagacaatgt agatgtgaaa
      481 taccgcgtct ggaaggcgga agagaaaatt gacaacgcgg aagttcgcta aactgttgtg
      541 aagtacggca ccccgttggt tggataaggc ggttacgccg catccaacat ctaacgcccg
      601 agccggttgc ctgatgcgac gctggcgcgt cttatcagta tacgccacat cattacaacg
      661 caatatccgc cacttctttt tgaacgggtt gcggtttcaa ctgcggtttc ggcgtactat
      721 ctgctgcttg cggtttgtcg tatttcagcc ccagcacttc gctggtgtac tgcaactcgc
      781 gttctgttgc cgctacatca ccgttcagct tgcgtccata agacggaacg atcgctttca
      841 acgtagcctg ccattgcggg ctggaaacgc gatcgccaaa tactttttcc agtagattca
      901 acataatcgg cgcggcggtt gacgcccctg gcgatgcccc cagcagcgcc gcaatggtcc
      961 cttgctggtc actgaccact tcggtaccca gacgcagtac gccacctttc tcggcatcac
     1021 gcttgataat ctgcacacgc tgccccgctt gccacaaacg ccagtcctct tttttcgctt
     1081 gcggatagta ctctttcaac gcttcaaaac gatcctcttc actcaacatc acctgactca
     1141 ccagatattt caccagatcg aaattatcca gcccgacgtg catcatcggc atcacgttag
     1201 aggtggtggt ggaactcatc agatcccaca atgaaccgtt tttgaggaat ttggttgaga
     1261 aggtggcaaa tggcccaaac agcactacgc gtttaccgtc cagaacacgg gtatcgatat
     1321 gcggaaccga catcggtggt gcgccaacgg atgctttacc gtaaactttc gccagatggc
     1381 gattaaccac gtctggattt tccgaaacaa ggaactgtcc gcccaccggg aaaccggcgt
     1441 agtctttcgc ttccggaatc cccgattcct gtaacagctt cagcgccgca ccgcccgcgc
     1501 cgataaagac aaatttggcg cggatgttct gtgcagtgcc gtttttcaga tcggcaacgg
     1561 taacggtcca ggtgttatcg tcattacgct ttagggcgcg gacttcgctg ctgagttgca
     1621 gcgagaagtt agatttcttc tgtaaggaag caattaactg gcgggtgatc tcaccatagt
     1681 tcacatcagt gccaatttct gttgcggatc gcgcccttcc atcactaacg gtgcccactc
     1741 tttgatctgc gcgtgatctt cagagtaacg cataccgcga aacagcgagc tttgttgcaa
     1801 cgcggcgtaa cgggcgcgca ggaaattaac gttatcctcg ccccagacaa agctcatatg
     1861 cggaacggta ttgataaatg aacgcggagt acgcagcacg ccgcgctcga cctggtgcgc
     1921 ccagaactgg cgggaaatct gaaatgcttc gttaatggcg actgcttttt caatactgat
     1981 gctgccatcg gcgttttgcg gggtgtagtt cagttccatc agtgcagaat gcccggttcc
     2041 ggcgttgttc tagccgttcg aactctcctg cgcgcccccc tccaggcgct ccaccatggt
     2101 catcgaccat tcaggctcca gctcgcgtca ataggtcccc aacgtggcgc tcataatgcc
     2161 gccgccaatc aacagtacat cagtttcctg ctcctcggac gctttcgctt ttgccgccat
     2221 cgaaacggca ttaagcccca cggccatcga gaagagcatg gcagtcactt ttttcatctt
     2281 gttaatgcct tacttttagt cgctttattg caggtgagat ttgcgcggca tcaacggtaa
     2341 ca
//
LOCUS       DAZOYF010000317         2334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZOYF010000317 DAZOYF010000000
VERSION     DAZOYF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.325"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..734)
                     /gene="ipaJ"
                     /locus_tag="ACHUOF_004306"
     CDS             complement(<1..734)
                     /gene="ipaJ"
                     /locus_tag="ACHUOF_004306"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFR0613209.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIG"
     gene            complement(931..1740)
                     /locus_tag="ACHUOF_004307"
                     /pseudo
     CDS             complement(931..1740)
                     /locus_tag="ACHUOF_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2087..>2334
                     /locus_tag="ACHUOF_004308"
     CDS             2087..>2334
                     /locus_tag="ACHUOF_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0613210.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT          670 a          595 c          400 g          669 t
ORIGIN      
        1 tatcctataa agttagaatt acttcttctt gcacctaatt ccatagttga aaaacaagaa
       61 taattttctc ctacagctgg atccatatag cttccatcag gtctacacaa cacccagtgg
      121 aggccgacag gaactccaat aaaggataca gcaacagctt ccaaaactct ttggttagaa
      181 gataacacat ctcgttgatg tactatatta catcccatgc caattaataa gtcccttgca
      241 tcgggataaa taaaactaat tacttgcgaa aaaatgttac tttcttcaac gacataagca
      301 ttcaatccaa gaagcctcgt agccatgact atcttatcag gcatagaata accggcatcc
      361 gcgatattat ctttgtgtat tctcggatta taattaccac tagttattaa gtataaatcc
      421 cgctcacaac gattatccaa atcaagagaa ctcttccttg tcatttcgga catagaccct
      481 ttgtattgtg gtattttatc aacccctaat tcctttgcag cacagagtaa actggcagct
      541 ccacaagaat tgtcatacat ctgcttcctc gccaaaaaac aaccttgttc gtttactcta
      601 ttctcattta ccctcacatc agtctgatga gatatcatat attctcttgg tatagccccc
      661 tgcaataaaa ccccatataa cataacacct gtatgaatac atccccgttt gcaaggcttc
      721 cgttgttccg acatcgacga gtaattgcag cacagtattg catccagcaa tgcgaactac
      781 tatgagaatt agctgaccgg gtccttattc cccgaacgaa ctttaaatcc aaaacctcat
      841 actcttttcc gtcgtcatca cgtccgtcac cataacaacc tttcagcatc caccctttta
      901 ttaagagtta atgtccatca gcatcagcaa ttaataccgc ctgatactga catatttttg
      961 tcgtatttac gtccataatc ggttattgtg atacaggtca caccagctta tttgctattg
     1021 ttacaataac caccttggtt gagagctgtt ttttcaactt tgttatcaac tcccccaact
     1081 ccttggtcag taaggctgcc gaccgagcac agtgaataga tagtatccgc aggtaccacc
     1141 atgcttactt aatactcaga aggcgaagtg ctctccaatt aactctgccg cactatgctt
     1201 ataagctgct tttcaatgcc gtctatcaac atgtccaatt cattcaatct gttgtactgg
     1261 taatccaacg cagtaatgat atatgcaggt attctttcat ccactccagt aacgtttcat
     1321 gcaaagcatt aatttacgcg ctacggaact tcaccagttg actgagcaaa cgatgtggga
     1381 ccagtactgc ctgcagttct tctattgtta cagcgatttc tttaccaaac agctgaacca
     1441 ccatcaagat aaagatagcc tgaacattca tcgcaccatt ttgttaccaa tggcaaaaga
     1501 tttaacgaac cgtgcctgca tcaggcggac tttttatccc tgctttgtta gctccctaac
     1561 cttgtacaga gagcctccat aagctcccat atcaagcaga catggttcgc agttactgac
     1621 atacaccaga aaactctgac agcagactca cttatcctcc acttcactca tatccttatt
     1681 ggtgagatga acctgaatga catgttttgc gacataaacg ccaaccagtg tatatttcac
     1741 ctgtagagat tctacaatct gtagcgttat gcatctaatg ttaggctcta tgatgtcaga
     1801 aatctgcgag actctacacc ttctgcattt acctttattt atcaccatgc tcctcagtta
     1861 acggagaacg tgttcattgc attttgtcac ttacatttcg cctttggtat cggggggata
     1921 taggttccaa cgttccgcat gagaacctga accggcttca cgccaccttg ataccagatg
     1981 ccgcacaggt agtcaacaag tgtcacgaac ggtgcaatag tgatccacac ccaacgcctg
     2041 aaatcagatc cagggggtaa tctgctctcc tgattcagga gagcttatgg tcacttttga
     2101 gacagttatg gaaattaaaa tcctgcacaa gcagggaatg agtagccgga cgattgccag
     2161 agaactgggg ctctcccgca ataccgttaa acgttatttg caggcaaaat ctgagccgcc
     2221 aaaatatacg ccgcgacctg ctgttgcttc actcctggat gaataccggg attatattcg
     2281 tcaacgcatc gccgatgctc atccttacaa aatcccggca acggtaatcg ctcg
//
LOCUS       DAZOYF010000318         2325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZOYF010000318 DAZOYF010000000
VERSION     DAZOYF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.326"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            61..282
                     /gene="kil"
                     /locus_tag="ACHUOF_004309"
     CDS             61..282
                     /gene="kil"
                     /locus_tag="ACHUOF_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR0613211.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            282..452
                     /locus_tag="ACHUOF_004310"
     CDS             282..452
                     /locus_tag="ACHUOF_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFR0613212.1"
                     /translation="MTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="ACHUOF_004311"
     CDS             527..802
                     /locus_tag="ACHUOF_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613213.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="ACHUOF_004312"
     CDS             902..>2325
                     /locus_tag="ACHUOF_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR0613214.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          720 a          531 c          528 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatattcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAZOYF010000319         2320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZOYF010000319 DAZOYF010000000
VERSION     DAZOYF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.327"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..763)
                     /locus_tag="ACHUOF_004313"
     CDS             complement(<1..763)
                     /locus_tag="ACHUOF_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0613215.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQL"
     gene            complement(1025..>2320)
                     /locus_tag="ACHUOF_004314"
     CDS             complement(1025..>2320)
                     /locus_tag="ACHUOF_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0613216.1"
                     /translation="ADNTTNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGD
                     KSDIIYKVNGLSIIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFY
                     KQCGVINPQNANTAYFGDTDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASS
                     AYIHKNNKLLYNLLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYI
                     LTLLNSMRNMEGNKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTL
                     DVCNYNSETKVLTSHHGIIRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQ
                     HMDDHLLHLIRSNAIAPTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLG
                     VDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPQ"
BASE COUNT          728 a          431 c          374 g          787 t
ORIGIN      
        1 caagttgctc attaaagttc tcccttagca atacgttgag aacttcatct tctatattaa
       61 gcaactggct aaggaaattt tctccttcag ccatgattcc caaatgtcct gttgcaaaaa
      121 gcgcgtacaa aactgcaccg actgagccat cggtatcgcc aaaataggtc ggcctggtac
      181 ttgtgggatc aacaggtgaa caattggaga aataggttct gtcagcgaac tggggtttta
      241 taattgtttc gaatttaata tcaaatacat tgtattgcga tatttcccag ttccagatgg
      301 gtttgtacga ctcagtaact gcaacatcat tagcattatc atttactgtc atattattaa
      361 gtaaataaac aatgctgcac caattaatat cggtattatc aagagggcaa ccgtaatttt
      421 tactattaat tgtcacaata aactgataac agttattcct acgtagaata tgagcgcgca
      481 tgcccgatag catactatat ttttctgaat gattcgtttg tatggtaaat gaaattttgt
      541 catcttgaat agattgttgg aaacgttcta tttgccaatc atcaattttt tccacaactt
      601 caccgagcat tgtcctggca atacttgata tatcatcacc tataccattt gcaatatacc
      661 caaacagttc tttagtgaag ttggtaacat gctcttgcac catatcattt gttgcgattg
      721 tcgttgtact gccattagtg cctgccacag ttccaatttt catcttttac ctcatattat
      781 tcaccgcgag gaatacatag ttaacatttt atcttatgac atacacctca tattcgatgg
      841 ggaaataatt atattttgca caaatcattc agccttaaag cataatatga aatttcttat
      901 ctcatagcag agaaagtccc taaatattgt agaggcgtca tcagtacata cctctacaat
      961 cgattaagaa aacctatgaa atactggcaa aattaaaaag agataccaca gcactcatga
     1021 agttttattg aggttgaaaa caactaagcc ctgggacacc ttttttatca ccacgttcgt
     1081 taggtgctat cttccaagaa tttagcccca tggtataatt atcaattttt tgatgctggt
     1141 ctacccctaa tccataatgg ccaaatttct gtttagtttt tttgagcgtc tgtccacatt
     1201 tgaagatatc ttcaggtttt ggtctgaaag cagtagtgtt attaaaataa ttttcatata
     1261 caggtgtggg tgctatagca ttgcttctaa ttagatggag aagatgatca tccatgtgtt
     1321 gtttatgttt tttattacat atatttgcta attcctcagg atcaattggg tctttcatct
     1381 gattaaatgg aacctgcaaa gcaccgagac aataacattg attttcttca tttcttatta
     1441 ttccgtgatg actggttaaa actttagttt ctgagttata attacaaaca tcgagcgttt
     1501 taattaagtt atacgtatca ccggcagaga gtttataatt ttctagtcgt gcagtatagt
     1561 taccattaaa attgatttca tgatttccag ccagaacgat gacattttta ttaatacggc
     1621 tatctttatt accctccata tttcgcatag aatttagcaa agtaaggata tatttatcac
     1681 caaaaatagt actaaatcta tcgcctgtat gatccccaag gaagatacaa ggggttatcg
     1741 catcgtcctt taaaaaaaca tgttgatgca actcattcaa tatcatgtct ctcgtattta
     1801 ataaattgta gagcaattta ttatttttat gtatgtatgc agatgaagcc atgtcttcgt
     1861 gttttaataa ttcacataat aaactccaac ccttttccct gatgcctata tgccctgaaa
     1921 cgagcaatga ataaagtaca gcgcctactc ttccatctgt atcgccaaaa taagccgtgt
     1981 tggcattttg tgggttaatc acaccacatt gtttataaaa gtctatttta gaaaacacag
     2041 atttaacata gctatcaaac ttatccttag gaaacgtctc tttagagaca tcccaacgcc
     2101 agtcagcttt aaaattaccg ctaagaagag taaagaaatt ttcaggtatg atagaaagcc
     2161 cattaacttt ataaattatg tctgatttat ctccggagaa attaataaaa taataaccat
     2221 ccttacactc aatcttaact gtaatgggag acgataattt tatcacatgg ttattgtctg
     2281 gtataattgt aaagataaac gtgttagtag tattatcggc
//
LOCUS       DAZOYF010000320         2269 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZOYF010000320 DAZOYF010000000
VERSION     DAZOYF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.328"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            65..583
                     /gene="mddA"
                     /locus_tag="ACHUOF_004315"
     CDS             65..583
                     /gene="mddA"
                     /locus_tag="ACHUOF_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR0613217.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            580..1029
                     /locus_tag="ACHUOF_004316"
     CDS             580..1029
                     /locus_tag="ACHUOF_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0613218.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1030..1263)
                     /gene="ydcY"
                     /locus_tag="ACHUOF_004317"
     CDS             complement(1030..1263)
                     /gene="ydcY"
                     /locus_tag="ACHUOF_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR0613219.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1349..1522)
                     /gene="ortT"
                     /locus_tag="ACHUOF_004318"
     CDS             complement(1349..1522)
                     /gene="ortT"
                     /locus_tag="ACHUOF_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR0613220.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1717..1812
                     /gene="yncL"
                     /locus_tag="ACHUOF_004319"
     CDS             1717..1812
                     /gene="yncL"
                     /locus_tag="ACHUOF_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR0613221.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          562 a          510 c          542 g          655 t
ORIGIN      
        1 gttgcttcgg gctgttttcg atagtattga taacatcatc atcattattt tattgaggcc
       61 agacatgtcc atccgttttg cccgcaaagc cgactgtgct gccattgcgg aaatttataa
      121 ccacgccgtg ttgtatacgg cggctatctg gaatgaccaa acggtggatg cagataaccg
      181 cattgcctgg tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga
      241 tggcgtagtt acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca
      301 taccgtggaa cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa
      361 attgttaagt cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg
      421 gatcgaatcg caaaatcagg cttcgctgca tctgcaccag tcgctgggat ttgtcgtcac
      481 cgcacaaatg ccgcaggtag gcactaaatt tggtcattgg ctggatctga catttatgca
      541 gttgcaactc gacgagcgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt
      601 gcttttttga ttgccgccgg aattggtctg gtagtccaga acacgctgat ggcgcgcatc
      661 acccagacct cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg
      721 ctctttgttt ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc
      781 agcgtgcgct ggtggacact tatacctggc ttgctgggtt cgttttttgt cttcgccagt
      841 atcagcggat atcagaatgt gggagcggcg acaactattg cggtactggt cgccagccag
      901 ctgattggcg gtctggtgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg
      961 ttcggcccaa tatgtggcgc aattttactt gtggttggcg cctggttggt ggccagacgc
     1021 tcattttaat cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc
     1081 ttctttatgc ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta
     1141 gcgatcttcc cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg
     1201 ggcaatgacg ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga
     1261 catactttct ccttagccac ttgtgaaatt tatcgattaa tccaaatgat ataagtctgc
     1321 gtggtgccgt caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga
     1381 aactcatcgg ccatgtggcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat
     1441 cgtgagaaag aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa
     1501 gcatatgttg atagagaaac atgtacgggc gtccagccat atgaataaaa cgcgcgcaaa
     1561 tatgcgccat tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa
     1621 cagcaacttg cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta
     1681 ctatacacat aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta
     1741 gtactgataa atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc
     1801 tggtttattt gatgtagtga ttttgcacgc agcacgtact gtcagagaaa tcaactctct
     1861 gacagtacgc aagatcttct catactctcc aagtttgcaa cattatcctt aatatttact
     1921 tctttttgct ggcatctaca ttgaaatgag tagaaaacat ccccctactg tcgtctatac
     1981 aaacagctta ctgattcaaa cggaatcttg ttttcacatt tttctatcac gaatacttta
     2041 tacgcgcggg agtctataaa tgctttatac tttcaaggaa taacgtaaat ataaaaagat
     2101 ggcgtaaagt atatgcatgg caggatgttt gcaacctcct tgatgacgct gccccattga
     2161 aattgcaaac cccgccctgg ttatcttgcc catttatcat gcaaaacaga taattatagg
     2221 cctctgaata cctcagaaag attgaatttt ttatagtccc tctcttgaa
//
LOCUS       DAZOYF010000321         2250 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZOYF010000321 DAZOYF010000000
VERSION     DAZOYF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.329"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            72..302
                     /locus_tag="ACHUOF_004320"
     CDS             72..302
                     /locus_tag="ACHUOF_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613222.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            280..780
                     /locus_tag="ACHUOF_004321"
     CDS             280..780
                     /locus_tag="ACHUOF_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR0613223.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            780..2198
                     /locus_tag="ACHUOF_004322"
     CDS             780..2198
                     /locus_tag="ACHUOF_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR0613224.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
BASE COUNT          570 a          534 c          594 g          552 t
ORIGIN      
        1 gttggtctgc ctaaccagac cgcgtgataa ccagagggag cttcggctcc cgtttttatc
       61 tggagacaag catgacacac atgatctttc gtcatggcga catgaagaag tggaaaggcg
      121 ttggctacga ctttgaaatc gtgaaagccg aagagcttca ggaatatctg gatgctggct
      181 ggttttcaca tcctgatgac cttttgaagg atgttgcaga gccagagcca gaagaaaaac
      241 agcgtaaaaa gcctggtcga aaacctaagg cggcagcaga tgaacctgac aacgaagggt
      301 gatttagttc ttgcggcatt acgtaagctc ggtgttgcat caaatgccac gttaaccgat
      361 gtcgaaccgc agtctatgga agacggcgtc aacgaccttg aaatgatgat ggctgaatgg
      421 cttggcggtg atgcgtcacc tggtatcaac gttggctaca tttttgctga tgcagatgtt
      481 gctccggatc cgggcgatga gcacggttta tcaaataacg ctatcaatgc cgtcattttc
      541 aaccttgcct gccgaattgc tccagattat gcgctggaag cgtctgcaaa acttataacc
      601 actgccagat acgggaaaga gcgactcgtc aaactgtctg caatggacag ggcaaaagca
      661 gctaaatgta agtccggtta tccaaaccgt atgcctgttg gtagtggtaa ccagttggcg
      721 aagtggaatg gttggaatta cttccaccga aaggaacctt gcgataacgg gagcgaataa
      781 tgcctattca gcaacttccg cttatgaaag gtgtcggcaa agacttccga aacgccgact
      841 atatcgacta tctgccagtg aatatgctgg ctacacccaa agaaatcctg aacagcagcg
      901 gatatcttcg ctcattcccg ggcattgcca aacgttctga cgtgaacggt gtatctcgcg
      961 gcgtcgagta caacatggcg cagaatgctg tttatcgcgt gtgtggcggc aagctgtaca
     1021 aaggagaaag tgaagtcggt gatgttgccg gaagtggtcg tgtatcaatg gcgcatggtc
     1081 ggacatcaca ggcggtaggc gttaatggtc aactggtcga gtatcgctat gatggcacgg
     1141 tcaaaaccgt ctcaaactgg cctacagaca gtggattcac tcagtatgag ttaggttcag
     1201 ttcgcgacat tacgcgctta cgtgggcgtt atgcgtggtc aaaagacgga actgattcat
     1261 ggtttatcac tgaccttgaa gacgaatctc atcctgaccg ttacagcgca caatatcgtg
     1321 ccgagtctca gcctgacggc atcatcggta tcgggacatg gcgagacttc atcgtctgct
     1381 ttggttcatc gactattgaa tatttctccc tgactggcgc aaccaccgtt ggtgccgctt
     1441 tgtatgttgc acaaccatcg ctgatggtgc agaaaggcat tgccggaact tactgcaaaa
     1501 cgccattcgc tgattcgtat gcgtttatca gcaatccggc aacgggtgcg ccgtctgtgt
     1561 atatcatcga ctccggtcag gtgtcaccaa ttgccagcgc gagcattgag aaaatcctcc
     1621 gctcctacac tgctgatgaa ctggctgatg gtgtgatgga atcgctgcga tttgatgctc
     1681 atgagttgct gattatccac cttccgcgtc acgtcctcgt gtacgacgca tcttcaatcg
     1741 ccaatggccc acaatggtgt gtgctgaaaa caggtctgta tgacgatgtg taccgcgcta
     1801 tcgacttcgt ttacgaaggc aatcagataa cgtgcggcga taagctggaa tctgttaccg
     1861 ggaaactgca attcgatatc agcagccagt acgacaagca acaggaacac ctgctgttta
     1921 ctccactctt caaagcggat aacgccagag tgttcgacct tgaggttgaa tcgtcaactg
     1981 gcgttgcgca gtacgccgac cgcctttttc tctctgcaac cactgactgc atcaattacg
     2041 ggcgtgagca gatgattgag cagaatgaac cgttcgttta cgacaagcgt gttttgtgga
     2101 agcgagtagg gcgcatcagg aaaaatgtcg gcttcaaatt gcgcgttatc acgaagtcac
     2161 ctgtcactct gtctggcgct cagataagga ttgagtaatg gctgattcga atctcaatgt
     2221 gccggtaatc atccaagcta cgcggctcga
//
LOCUS       DAZOYF010000322         2239 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZOYF010000322 DAZOYF010000000
VERSION     DAZOYF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.330"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(121..546)
                     /gene="arsC"
                     /locus_tag="ACHUOF_004323"
     CDS             complement(121..546)
                     /gene="arsC"
                     /locus_tag="ACHUOF_004323"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR0613225.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(559..1848)
                     /gene="arsB"
                     /locus_tag="ACHUOF_004324"
     CDS             complement(559..1848)
                     /gene="arsB"
                     /locus_tag="ACHUOF_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR0613226.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1902..2237)
                     /gene="arsR"
                     /locus_tag="ACHUOF_004325"
     CDS             complement(1902..2237)
                     /gene="arsR"
                     /locus_tag="ACHUOF_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR0613227.1"
                     /translation="MQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          595 a          615 c          560 g          469 t
ORIGIN      
        1 agtaagttgg cagcattacc tgaatttgca cggttttgta ggtcggataa gacgcgttag
       61 cgtcgcatcc gacataaaca aagcgcactt taccaactat cggcagtgga tatcgccgct
      121 ttatttcagg cgcttacccg cttcatcaac cactttctcg ccatcttcct tggagaatgc
      181 gcctttttgc gcatctggca gaatttccag caccacttct gaagggcggc acaggcgagt
      241 tcccagcggc gtcaccacaa tcgggcgatt aatcagaatc gggtgctgaa gcataaagtc
      301 gattaaccga tcgtcagtaa atttatcttc cgcaaggccc agctcctcat acggttcgac
      361 gtttttacgc agcagcgcgc gtacggaaat ccccatatcg gcaatgagtt tgaccagttc
      421 atcgcgcgtt ggcggagttt ccagataatg gataatggtc ggttctgtgc cgctgttgcg
      481 gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa tggtaatgtt
      541 gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg cagccagcgt
      601 cacaaacagc acaggcaggg tcatgataat ccctgtacgg aagtaatatc cccagctgat
      661 agtcatattc ttctgcgaaa gtacgtgcag ccagagcagc gttgccaggc taccaatcgg
      721 ggtaattttc ggccccaaat cgcagccaat cacgttggca taaaccatcg cttctttgat
      781 aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg tcggcatatt
      841 gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg cggcccacag
      901 gccgttatcc gccagcacgt tgagtacgcc agaaagatat tccgttaatc cggcattgcg
      961 caggccataa accaccagat acatgccgag cgagaagatg acaatctgcc agggggcacc
     1021 gcgcaggact ttacccgtat taatcgcatg accgcgttta gcgacgacaa ataatatcag
     1081 tgcgcccaca gctgcaatgg cgctcaccgg aatgccgagc ggttccagga cgaaaaatcc
     1141 caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat ctttgatcgc
     1201 ttctgcggga gatttcagca gcgccatatc gtagttctgc ggaatatctt tgcgaaaata
     1261 gagatgtagc atcaccagcg tggcaacaat cgcggcgata tccaccggca ccatcaccga
     1321 ggcgtattcg cgaaagccga ggccaaagaa atcagcggaa acgatattca ccaggttgga
     1381 gacaataagc ggcaggctgg cggtatcggc aatgaatccg gccgccatca cgaacgccag
     1441 cgtcgtgcct ttactgaatc ctaaagccag cagcatggcg atgacaatcg gcgtcaaaat
     1501 aagcgccgcg ccatcattgg caaacagggc cgcaacggcg gcaccgagca ggacaatcca
     1561 ggtaaacagc aagcgaccgc gtccattgcc ccagcgtgaa acgtgcagcg ctgcccattc
     1621 aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcaataa acgccgccgt
     1681 cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatgaacca cgcccgtaac
     1741 caacgccagt accgcgccga gcgttgcact ccagccgatg cctaaacctt tcggctgcca
     1801 gataaccaat acgatggtca ggacaaagat agcgcctgcc agtaacataa tgcctcccgg
     1861 ataaaacaca tgtgcaaatc catatgtgtt tagctaaatt tttaactgca aatgttctta
     1921 ctgtctccgg aacagttttg tctggccagg ttgcggacaa ttgcctgaat cttttcctgt
     1981 tcacatcgcc aggcttcatc aataattttc gccgcccatg ctggaatatg cggcgataag
     2041 cggtaatgaa cccacttacc ttgcttgcgg tccagcaata gcccgctttc acgcagcaat
     2101 gccagatggc gggatatctt gggctgcgac tgatcgaggg cagtgcagag atcgcagacg
     2161 cataactctc ccagttcgct aagcagtaaa acgatgccca gacgggtttc atcagcaaga
     2221 attttgaaca attggatgg
//
LOCUS       DAZOYF010000323         2234 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZOYF010000323 DAZOYF010000000
VERSION     DAZOYF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.331"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..373
                     /gene="tnpA"
                     /locus_tag="ACHUOF_004326"
     CDS             <1..373
                     /gene="tnpA"
                     /locus_tag="ACHUOF_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFR0613228.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
     gene            373..720
                     /gene="tnpB"
                     /locus_tag="ACHUOF_004327"
     CDS             373..720
                     /gene="tnpB"
                     /locus_tag="ACHUOF_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0613229.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            740..1969
                     /locus_tag="ACHUOF_004328"
                     /pseudo
     CDS             740..1969
                     /locus_tag="ACHUOF_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1973..2132
                     /locus_tag="ACHUOF_004329"
                     /pseudo
     CDS             1973..2132
                     /locus_tag="ACHUOF_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          544 a          595 c          620 g          475 t
ORIGIN      
        1 gcggcctaat tttccttatg aatttaaaat cgctttagtg gagcagtcac tgcagcccgg
       61 agcctgtgtg gcgcagatcg cccgtgaaaa cggaatcaac gataacctgc tcttcaactg
      121 gcgccatcaa taccggaaag gtggcctgct gccttccgga aaaaatatgc ctgcactgct
      181 tcccgtgacg ttaacgccgg agccggataa taaaatcccg gctccggcac aggaaccaga
      241 gcagataaat acaccgtccg acagtctgtg ctgtgagctg gttctgccgg ccggaactct
      301 caggcttaaa ggtaaactga cgccggcgtt attacagata cttatccgcg aaataaaagg
      361 gagcagccac tgatgatatc tttccctgca ggttcgcgta tctggctggt tgcaggtatc
      421 accgatatgc gaaatggctt taacggcctg gtctcaaaag ttcagaacgt cctgaaggat
      481 gacccgttct ccgggcatct gttcatcttc cgcggacgcc ggggtgacca gataaaagtg
      541 ttgtgggctg acagtgacgg actgtgcctc tttaccagac gcctggagcg gggccgcttc
      601 gtctggccgg tcacccgtga cggcaaggtg caccttactc cggcccagtt atccatgctt
      661 cttgagggca tcgactggaa acacccgaaa cgaacggaac gcgctggcat ccgcatataa
      721 cccgttgtaa agtgaggata tggacacctc acttgctcat gagaatgccc gcctgcgggc
      781 actgttgcag acgcaacagg acaccatccg ccagatggcg aaatacaacc gcctgctctc
      841 acagcgggtg gcggcttatg cttccgaaat caaccggctg aaggcgctgg ttgcgaaact
      901 gcagcgcatg cagttcggta aaagctcaga aaaacttcgc gcaaaaaccg aacggcagat
      961 actggaagca caggagcgaa tcagcgcact tcaggaagaa atggcggaaa cgctgggtga
     1021 gcaatatgac ccggtactgc catctcccct gcgccagtct tcagcccgta aaccgttacc
     1081 agcctcactt ccccgtgaaa cccgggtcat ccgtccggaa gaggaatgct gcccggcctg
     1141 tggtggcgaa ctcagttctc tgggatgtga tgtgtcagag cagctggagc ttatcagcag
     1201 cgcctttaag gttatcgaaa cacaacgtcc gaaactggcc tgttgccggt gtgaccatat
     1261 cgtgcaggca ccggtgcctt caaaacccat tgcacgcagt tatgccggag cggggcttct
     1321 ggcccatgtt gttgctggaa aatatgcaga ctatctgccg ttataccgcc agtcagaaat
     1381 ataccgtcgt cagggcgtgg agctgagccg tgccacactg gggcgctgga caggtgctgt
     1441 tgctgaactg ctggaaccgc tgtatgacat cctgcgccag tatgtgctga tgcccggtaa
     1501 agtccatgcc gatgatatcc ccgttccggt ccaggagccg ggcagcggta aaacaaggac
     1561 tgcccggctg tgggtctacg ttcgtgatga ccgtaacgcc ggttcggaaa tgcctccggc
     1621 ggtctggttc gcgtactcac cggaccggaa aggtatccat ccacagaatc atctggccgg
     1681 ttacagcgga gtgcttcagg ccgatgctta cggtggttac cgggcgttgt acgaatccgg
     1741 cagaataacg gaagccgcat gtatggctca tgtccggaga aaaatccacg atgtgcatgc
     1801 aagagtgcca accgacatca ccacggaagc cctgcagcgt atcggtgaac tgtatgccat
     1861 cgaggcagaa gtccggggat gttcagcaga acagcgtctg gcggcaagaa aagccagagc
     1921 cgcgccactg atgcagtcac tgtatgactg gatacagcaa cagatgaaaa ttcattcgct
     1981 gaaaatggaa tgtctccacg gagaacacta ttatccatcg ggaaatagtg cgggcaacag
     2041 tgtttagtta tatcgagtgc gattacaatc gctggcgacg gcaccgttgg tgtggtggtc
     2101 tcagtccgga acaatttgaa aaccagagcc tcacttacga ctgtgtccat attatgtggg
     2161 taggatcaat aaagacgttt tatatgtgtc tgcttgcagc cagaaaattt ataccctgca
     2221 ggtatcataa acat
//
LOCUS       DAZOYF010000324         2230 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZOYF010000324 DAZOYF010000000
VERSION     DAZOYF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.332"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..222)
                     /locus_tag="ACHUOF_004330"
     CDS             complement(<1..222)
                     /locus_tag="ACHUOF_004330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613230.1"
                     /translation="MFFSLSVLNLEDHLGLVISPQSPLSAGYITLRACRRSLPLRFST
                     VTPGGIVGFLIVSYRFNIPDRLAVHLSIHA"
     gene            651..2159
                     /gene="ltrA"
                     /locus_tag="ACHUOF_004331"
     CDS             651..2159
                     /gene="ltrA"
                     /locus_tag="ACHUOF_004331"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR0613231.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          572 a          548 c          657 g          453 t
ORIGIN      
        1 ggcatggatg cttaaatgaa ccgcgagtct gtctggaata ttgaaccggt aactcacgat
       61 gagaaaccca acaatcccac cgggtgtgac ggtggagaac ctgagcggca gtgacctgcg
      121 gcatgcccgc agggtgatgt aacccgctga caacggggat tgaggcgaga tcactaagcc
      181 gagatgatcc tcaaggttaa gtactgaaag gctgaagaac atgaacccgt taatccgcct
      241 ctgtgggttg aaaacgtcac cacggcctac gtgatctgac aggccgtgca ggaggaactg
      301 gcagtgatac gtaagcactg ccggtcgaag gtgttttgac atgtatgcga aacaccgggg
      361 cagcagcgtc tatcacactt gcgttgctga cttctgccaa cttgcggcaa gcaaggataa
      421 agagtgcgac gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac cggggaaggt
      481 cagcgacgga tgttaagggg gcatggctcc gatgacgcgc tggctggcgg agcttccgta
      541 gtagtccgcg atggggaaag cccattacat ggcgaaggga agcagtttga atgtgtttgc
      601 gacgtgaatt aactgaccta acgaggtgaa gacctttgat aatcagcgaa atgcaacgca
      661 agcttgccac atgggcagcc accgatccgt ccctacggat tcaacggctg ctgcgtctga
      721 taacacaacc agaatggctg gctgaagcgg cgcggatcac gctttcatca aagggggccc
      781 atacccccgg cgttgatggc gtgaacaaaa caatgctaca ggccagactg gctgttgagc
      841 tgcaaatcct cagggatgaa ttactctcag gccactacca gcccttgccc gccagacggg
      901 tttacatccc taaaagcaac ggcaaactgc gaccactggg tatccccgcg ttgcgggatc
      961 gtattgttca gcgggccatg ctgatggcga tggagccgat atgggagagt gattttcata
     1021 cgctctcata tggcttccgg cctgagcgca gtgtccacca cgcgatccgc acggtgaaat
     1081 tacagctcac agactgtggt gaaacccggg gacgctgggt gattgaaggc gacctgtcca
     1141 gttacttcga caccgtacat catcgactgc tgatgaaggc tgtacgccgc aggatcagtg
     1201 acgcacgttt catgactctg ctgtggaaaa ccatcaaggc gggacatatc gatgtcggtc
     1261 tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt tatatcgccg ctattatcga
     1321 acatcatgct gaatgagttc gatcaatacc tgcatgagcg ctacctgagc gggaaagcca
     1381 gaaaagatcg gtggtactgg aataacagta tccaacgggg ccgaagtacg gcggtcagag
     1441 aaaactggca gtggaaaccc gcggtggcgt actgccgcta tgccgatgat tttgtcctca
     1501 tcgtcaaagg caccaaagca caggcggaag ccatcaggga ggagtgtcgg ggtgtgctcg
     1561 aaggcagtct gaaactcagg ctgaacatgg ataagactaa aatcacccat gttaatgacg
     1621 gctttatctt tctggggcac aggatcattc gcaaacgcag tcgttatggc gagatgcgag
     1681 tggtctcaac gatcccgcag gagaaagcca gaaacttcgc cgcatcgctg acagcactgt
     1741 tatcaggcaa ctacagtgaa agcaaagtcg atatggctga acaactcaac cgaaaactga
     1801 aaggctgggc catgttctat cagttcgttg attttaaggc caaagtcttc agttatatcg
     1861 accgtgtcgt gttctggaag ctggctcact ggctggcccg caaataccgt acaggtatcg
     1921 cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg tcagagcaaa acgtgggttt
     1981 tatttggtaa aaccaatcac ggcaagctca gcggcgaaat actgtaccgg ttggtggggc
     2041 aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa tccctatctg aggacggaga
     2101 ccagaaacac gtatacatcg cgctttacag aagtggcaat ggcgttcgcc agcatttaaa
     2161 tggagagccg gatgcgctga aaggtgcacg tccggttcgg ggaggagagg cagggaaata
     2221 gtccgactac
//
LOCUS       DAZOYF010000325         2211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZOYF010000325 DAZOYF010000000
VERSION     DAZOYF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.333"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            364..1554
                     /locus_tag="ACHUOF_004332"
     CDS             364..1554
                     /locus_tag="ACHUOF_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HFR0613232.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(1944..>2211)
                     /locus_tag="ACHUOF_004333"
     CDS             complement(1944..>2211)
                     /locus_tag="ACHUOF_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR0613233.1"
                     /translation="SGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANK
                     EQIEKIYPIVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
BASE COUNT          619 a          456 c          485 g          651 t
ORIGIN      
        1 ttcaatatta atttattatt tttcaaagtc gcactatata ggcatttact tccgcagtac
       61 caccgatagt atcgatatca aatcagcctg cggccgcgat aactttcgta acattctgcc
      121 agcggctttg ttgaataaat caggttcaag ataatcgtct ctgcatcagg ttagagtttc
      181 aggcaataca tcctctttct ggcatacctg ccactctcgc accatggtca tagcctttac
      241 gatctgcata ctcaccagac cagagcccgc tcccttttag gctgaggaat ccccagaaaa
      301 gaagacaggc atagagtttt gtgatctact gattgcgcta acaaattcaa tgagatcacc
      361 tctatgcctg cccgtaaagt atgccagact tttttccgta atgctttagc ttccacgcat
      421 cagtatcgac aaaatgccat tattgattct gctgctgctt tagtcggtgg cgcatctctt
      481 tctcttacca gcattggccg gcatttaccc ggtcctgctc gcgtgaaaga taaaataaaa
      541 cgcgttgact gtctgttggg taataaacga cttcataatg atatccctct gatattcaaa
      601 aatattacat ccatgatgac aaataaactg tcgtggtgtg ttatcgctgt tgactggagc
      661 ggttaccctt ttcaggaata tcatgtcctt cgtgccagcc tcctctgcga cggtcgctct
      721 atccccctga tgagtcaggt cttcccgtca aaaaaaaata acgaggcggt ggaaatcgct
      781 tttctggatg cgctttccgg ggccatttct ccccggactc gtgttgttat tgttactgat
      841 gctggttttc aaagtgcatg gttccgtcat atcaaatcac agggatggga ttttattggt
      901 cgtatcaggg gggtagtgaa gttcaggctg gatagtgaca aagataagtg gctggacata
      961 aaaatgtgca gggggagttc agaggcaaaa tacctgggga cgggaaccct ggcccggaaa
     1021 aaacgctcgc agtgtgaagg acatttttat ctttataaac attcacctaa aggcagaaaa
     1081 agcaggcgtg ccagagggag gccgggctta ccaacaacgg aaaaagaaca aaaagccgca
     1141 ggcagagaac catggttaat attcagcaat acagctgagt ttaatgccaa aaaaatcatg
     1201 aaactgtaca gtcgaagaat gcagatagag caaaactttc gggatgaaaa aagtgaacgc
     1261 tttggttttg gccttcgggc gagcagaagt cggaggggag agcgttttct ggtattaagt
     1321 ctactggtta cactggccag tatcgtactc tggctacttg gttatcattt tgaaaataaa
     1381 ggatttcatt taaagtatca ggctaatagt ctcaaaaaac gaagagtatt gtcttttctg
     1441 acgctggctg aaaacgtcct gagatttaac cctgaacttc tacgacgagc acaacctgaa
     1501 aagatattgt gccggttggc cagcacttac cgaagtatgg tgctggctta ctgatgaaaa
     1561 ttttctgggg atccctcagc ctttcgggga ggaatgaggg ctctgatatt cttttgccgt
     1621 agttcatcgt gaaaccgtcg gatgtcattt ggcctgagtg acccatgcct cccaaaagca
     1681 gagtcgctag tgttatgtaa ttaggcggtt aaacttcaat tctcagtgtt aagtttgggt
     1741 aaacgcttgt aaatggtatt gctaattttt taattgacta atattagaaa cggttagtgt
     1801 gattgtatat agatatttga gtgtaaggta tttaaagggg cattcagccc ctccgtctat
     1861 ttgtgattgc atccattgat gtgtgtatta ttttgagctt gaaaaaggga ggcgctcttt
     1921 aataataaat aaatctatca aagctaaata ttaaatggaa gccacccttt tttctctgat
     1981 agttttatta tcaccaaacc atttatttca gctatcgaat ctggattcgg ccaggaaggt
     2041 aaactcttca caattggata aatcttctct atttgttctt tatttgctgg tttgcaatta
     2101 cagattccat agtaattcat aaatgcaacc atcctaaaat agttgccacc atcccaccaa
     2161 aaaaaagatt ttccaaaagt gtctgcgctc atggagagca tatttcctga g
//
LOCUS       DAZOYF010000326         2199 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZOYF010000326 DAZOYF010000000
VERSION     DAZOYF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.334"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(133..399)
                     /gene="ospE2"
                     /locus_tag="ACHUOF_004334"
     CDS             complement(133..399)
                     /gene="ospE2"
                     /locus_tag="ACHUOF_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HFR0613234.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(581..982)
                     /locus_tag="ACHUOF_004335"
                     /pseudo
     CDS             complement(581..982)
                     /locus_tag="ACHUOF_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1411..2199
                     /gene="virF"
                     /locus_tag="ACHUOF_004336"
     CDS             1411..2199
                     /gene="virF"
                     /locus_tag="ACHUOF_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HFR0613235.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          677 a          389 c          407 g          726 t
ORIGIN      
        1 atcgaggata gatggagtta ctcctctctg aaatagaatt actgctctac tttttctgtt
       61 tccagaaatt attagtaaca aaccctgata tattcttttg aaaatggagc ttaaacgagt
      121 ctgaaattaa cttcagaaat cagaataccg ttggctttgt ttttcatcaa ttccggcttc
      181 atttatcttt gtcaaccatt gcatttctgt agcagtgaat ttcaacgttt tcgtccggtt
      241 ggcagcaggt atttgtaatt cgtatatagc tgctttttta ttgaaaacag tatatccatc
      301 tgtagtaaca gtagaagata ataatacagg atttgatact tcaaggatga tattttcttg
      361 tttttgtgga aggcaaggga atatagtttg tgtaagcata ttgcttcctt atgttatatt
      421 ttagtaaaga atttattttt tacattatag tcggctaaat gaagtttact gtaaaaaaac
      481 gatacttttt tcgtaagcga tgagatgtgg agccagaaaa atggctgcgt tacgtcattg
      541 aactgtcctt gacccacgat attcagacag tcgttgtatc ttaagttgac attacccgct
      601 gacgtcgata ttcccttggt gagagataac ccaacgcact atgcggatgg ttttcattgt
      661 aatgctcgat cgccactgca aggttatgca atgccgttct tggtttcggc atgaacgcga
      721 tgcagtcttc cttcatcgtt ttcatgaacc gttctgctat tccattactc tgagggctac
      781 tgactgccgt cgtgcagggc tccagattca actctctggc gaactgccgt gtttcatgag
      841 ccgtataagc cgaaccgtta tcggccactg gattggccgt tccggtcctt tatcgccata
      901 tcgctcctga catgacatcc tgcaccgttg ctttgtcata atctcccgtg cctgctgccc
      961 agtctatggc ttcacgatcg caatatggaa cgtaacgctg tcatcgggag aatcgtcaat
     1021 gcggcattcg gttaacgctt accaatatca acaccaaccg gatttgtgga gcctccagtc
     1081 tgaaggggct ttatgcgttc cgtataggat attatgatgc tggagttttg cgaagctcca
     1141 ctcctttccg aacatttaca cagatattgc ctaagaaaag tgaaaccata atatggttat
     1201 agtccctttc agtgcaaata cttagcttgt tgcacagaga aatagaagct gcataagctc
     1261 tttcttcaaa aaatgtaaat aaagttaaat ataggaaaaa ttacttaatc tatcttaata
     1321 acggaaagat tttgtataca atcactgtta cagaaatttc ttagttactc tgtaaacact
     1381 aaatatagtt tggtatattc tgttgaattt atgatggata tgggacataa aaacaaaata
     1441 gatataaagg ttcgcttgca taactatatt attttatatg caaaaaggtg ttcaatgacg
     1501 gttagctcag gcaatgaaac tttgactatc gatgaagggc aaattgcttt tatagagcga
     1561 aatatacaaa taaacgtctc cataaaaaaa tctgatagca ttaatccatt tgagattata
     1621 agccttgaca gaaatttatt attaagcatt attagaataa tggaaccaat ttattcattt
     1681 caacactcct attctgagga gaaaaggggg ttaaacaaaa aaatattcct cctctctgag
     1741 gaggaggttt ctatcgattt gttcaaatct ataaaagaga tgcctttcgg caaaagaaag
     1801 atctatagtt tagcttgcct tttatcagct gtttctgatg aggaagcttt atatacttcg
     1861 atatcgatag cttcttctct tagtttttct gatcagataa ggaagattgt tgaaaaaaac
     1921 atcgagaaga gatggcgtct ttctgatatt tcaaataact tgaatttatc agaaatagct
     1981 gttagaaaac gattggagag tgaaaaatta acatttcaac aaatccttct tgatattcgc
     2041 atgcatcatg cagcaaagct tttattgaat agtcaaagct atattaatga tgtatcaaga
     2101 cttatcggaa tatcaagccc atcttatttt ataaggaaat ttaatgaata ttatggtata
     2161 actccaaaga aattttactt atatcataaa aaattttaa
//
LOCUS       DAZOYF010000327         2187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZOYF010000327 DAZOYF010000000
VERSION     DAZOYF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.335"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(220..384)
                     /locus_tag="ACHUOF_004337"
     CDS             complement(220..384)
                     /locus_tag="ACHUOF_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613236.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="ACHUOF_004338"
     CDS             1286..1570
                     /locus_tag="ACHUOF_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR0613237.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="ACHUOF_004339"
     CDS             1558..2043
                     /locus_tag="ACHUOF_004339"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0613238.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataa
//
LOCUS       DAZOYF010000328         2183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZOYF010000328 DAZOYF010000000
VERSION     DAZOYF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.336"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHUOF_004340"
     CDS             <1..203
                     /locus_tag="ACHUOF_004340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613239.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSIH"
     gene            293..694
                     /locus_tag="ACHUOF_004341"
     CDS             293..694
                     /locus_tag="ACHUOF_004341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613240.1"
                     /translation="MNGNTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDY
                     LPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSR
                     DGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            696..1301
                     /locus_tag="ACHUOF_004342"
     CDS             696..1301
                     /locus_tag="ACHUOF_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFR0613241.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="ACHUOF_004343"
     CDS             1288..>2183
                     /locus_tag="ACHUOF_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0613242.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DAZOYF010000329         2164 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZOYF010000329 DAZOYF010000000
VERSION     DAZOYF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.337"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="ACHUOF_004344"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="ACHUOF_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFR0613243.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="ACHUOF_004345"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="ACHUOF_004345"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFR0613244.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..>2164
                     /locus_tag="ACHUOF_004346"
     CDS             1849..>2164
                     /locus_tag="ACHUOF_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR0613245.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYI"
BASE COUNT          587 a          482 c          511 g          584 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atct
//
LOCUS       DAZOYF010000330         2161 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZOYF010000330 DAZOYF010000000
VERSION     DAZOYF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.338"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="ACHUOF_004347"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="ACHUOF_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /gene="yniD"
                     /locus_tag="ACHUOF_004348"
     CDS             1128..1235
                     /gene="yniD"
                     /locus_tag="ACHUOF_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR0613246.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="ACHUOF_004349"
     CDS             complement(1288..2046)
                     /locus_tag="ACHUOF_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR0613247.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          650 a          481 c          453 g          577 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttctagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattagcca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 t
//
LOCUS       DAZOYF010000331         2152 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZOYF010000331 DAZOYF010000000
VERSION     DAZOYF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.339"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(248..467)
                     /gene="ymgF"
                     /locus_tag="ACHUOF_004350"
                     /pseudo
     CDS             complement(248..467)
                     /gene="ymgF"
                     /locus_tag="ACHUOF_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
     gene            complement(599..2122)
                     /locus_tag="ACHUOF_004351"
     CDS             complement(599..2122)
                     /locus_tag="ACHUOF_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR0613248.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          642 a          471 c          450 g          589 t
ORIGIN      
        1 attatcattt ttcaccttat tttcatgaca ttgatcactt tggggtgact attttgttta
       61 acaaataact cttcgacatc aaaattatta atagttgcca tggtaattat ttcggtgata
      121 tataaaaaca actgtgataa caccaaggtt gacaactatt tgcgcaataa ttcctttcct
      181 tcaatgatcc aggcgttcac tgccagcaac ccattcagcc gcatactgac agtgagaaac
      241 tgaaagatta ttgctgatcc ctgtggctaa cgagtctttc tcttaaatcg attaaccaga
      301 ttgatcgcca gccaaacaag ggcattaagc aaatttatcg tcacaagcac ttctcccagc
      361 ccggtactgg gttttgcaat aaggatgacc aggagggtga tcagcataaa tgcaatggaa
      421 aagatgatat agagagtgaa ataatccaga ttattactat tattcatgct actaatggca
      481 atcctgtcaa aactttcaaa caattatagc aaacaaaata atgaacaata gtaacaagag
      541 gcaacgagct aattagctgt ctataattac accgcactat ggccttactg atcaattttc
      601 actcaaccac aaccttcacc ttcggtttag aaaggataat tttgaccagg tcgatgtatg
      661 taacaggttt atagaaataa taaccctgct gaaatgtgat attattttgg ttcagatagc
      721 caagttgttc tttcgtttcg acaccttcag cgacgatact gatcgaaagt ttacgcgcca
      781 gatccaatac acaatccaga attcgggttg attctgggtc tgcgtttacg cggccaacga
      841 aactgtgatc aattttgata taatcaatat gcaggtcatg aagataagaa agccctgagt
      901 agccagtacc gaaatcatcc agcgcgatga caaaaccatt ttcatgcaga atgttcaacc
      961 gctgaaccag actttcatca acattcaatg gctcacgctc ggtgacttcc agaacaaggt
     1021 ttaaatcgcg gcgggtaaaa ctgtcacgga aatttaaaca ctcgtcgaca aacgtcggcg
     1081 aaataatatg cgaagcgcta aaattaattc caatatgaaa accttccggc agtttactcg
     1141 cgatagcgtt catctgtctg gcaacctgat tcatcaggct ttgtgtaagc ggaacgatta
     1201 atccggattt ttcagcaagt ggaataaatg ccgcgggtga aatatatcca ccgtgaggtt
     1261 gtttccagcg ggctaacacc tcaactcccc gcaatgtccc ttcccgacca tttaccacag
     1321 gttggtaaaa agggacgatc tccccattat ttattgcccg tcgcaggatc tcttcaggcg
     1381 ttgcgctttt attgaaatat ctatcaagca ggaaggcagc ggcgctggcg attaacagaa
     1441 taaatattaa aatcccaaag cctttatcga taagtctgta gaagctaaag agtggcggcg
     1501 ggttaaagat aatagtgaaa tgatattttg tggagtgaac cttctcggta tagggataat
     1561 gtccattaag cgtcatcaca tcagccgttg gtccaataat gtcatccgcc acgcgtaata
     1621 cataccttac ccctttcaag ggtacattaa gcgccccgcg aatatgttga tcacttatcg
     1681 taaccaaaat gcgcgtatct gcaaattggc tctgatagag caggataggt aatctattaa
     1741 cggtgtcgat tgccggagcc aacagtaaat tactgtccgg gacaacagga atacgagaca
     1801 gcaggacccg attcccaggc agggatgtac accagactat tccctgtttg agaatgatga
     1861 ttgtgcgcag gtgaggtttc agagctgctt cagtgccaag ctgatattgc tcctcgaggt
     1921 cgcattcctt cccggcaatg ttaaccgctg tttgagtcgc ctgtgacgct tcttcaagta
     1981 tatgattgat attgttggcg gcatatcttg ctcccgccag gtactctgct tttgcggaat
     2041 accagagctg gatgttcaaa atagcgacac cggtaataaa caagcatatc gcaacgagga
     2101 tgggtatgag tgtattgcgc atttgcttgt cccggccaaa gggtttaata ca
//
LOCUS       DAZOYF010000332         2132 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZOYF010000332 DAZOYF010000000
VERSION     DAZOYF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.340"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..132
                     /locus_tag="ACHUOF_004352"
     CDS             <1..132
                     /locus_tag="ACHUOF_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR0613249.1"
                     /translation="LKVDPLKCELRGGQMRFTGLKRGYRLAEQVMMHEPLARIRWCG"
     gene            319..556
                     /locus_tag="ACHUOF_004353"
                     /pseudo
     CDS             319..556
                     /locus_tag="ACHUOF_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            670..928
                     /locus_tag="ACHUOF_004354"
                     /pseudo
     CDS             670..928
                     /locus_tag="ACHUOF_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            977..1555
                     /locus_tag="ACHUOF_004355"
     CDS             977..1555
                     /locus_tag="ACHUOF_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613250.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1664..1922)
                     /locus_tag="ACHUOF_004356"
                     /pseudo
     CDS             complement(1664..1922)
                     /locus_tag="ACHUOF_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1906..2132
                     /locus_tag="ACHUOF_004357"
                     /pseudo
     CDS             1906..2132
                     /locus_tag="ACHUOF_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          670 a          378 c          522 g          562 t
ORIGIN      
        1 ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg
       61 aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc tcgaatacgg
      121 tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa
      181 aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta
      241 cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg
      301 ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat actgacgtca
      361 aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc aacagcgatc
      421 ctagatcggc tgctacacgc tgaatatcaa aggagagagt taccggttaa aagagaaacg
      481 taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa tggtggaaaa
      541 cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa
      601 cggtcaaaat gcttctgccg tgaaggaagg agaatgccat ctcatcactt atatctggcg
      661 aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca
      721 gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg
      781 ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat ggcatcctca
      841 tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca gtaatgggga
      901 aaatgatagc tggtttctga aaatatagag gaaaatgtga tatatatatc ctgttaatat
      961 ttaaggagaa tataaaatga aagtctcgtt taagtcttta ggctatattt ttcatgatat
     1021 atataataaa aaacacacta tcgatgagtt caacgatgtg gtaagaaaag cagttttatc
     1081 aggtaaaatt aacgagctga atgcatgtca taaagtggca atttttcttg ccgaaaaaga
     1141 caatgagata acaaagaaag acaaagccaa gataatagat acattaactg aaaactattc
     1201 cattgaattt caacaattaa tgaatattag tgagcgcacg ttaaattcat ctttatatat
     1261 tactcctggt gaaagtggtt ttgtcagttt tgttaatcgt gaaggaaaaa tatgtcatac
     1321 tgcatatgta aaatcatcag ataatagcat gacatattac catgcaaatg gtagcagcat
     1381 tgacaaatat attacggata tgtgcggcct tatttgtatg aggcatattg agtctactgg
     1441 cattattttt tatatgcttg atgaaaaagt tttatctgcc attgctgaat ttatgaatga
     1501 aaaaggatgg cgggctgcgt tttgttcagc aaagaactta tataaatgtg tttaatttta
     1561 gaaaaactat ttggtaaaat ccaacaagag tcgagaggtt cgcgttgctt cagcagggcc
     1621 tcagcctgtt caaccagact cagttgcagg tcgtgacgca gatttagtgg tggtggttga
     1681 ttgtcagata ccagccgatg atcttgtagt gcatatcatc agactttgag aagcatatgg
     1741 ttttgcgggt aagccgctta agatgtgtcc gcaggttcag attatgtcgc tctatccgct
     1801 gagtgcattt cttgcttata acatgactgg ttgaggacaa taaagttcgg tataccggct
     1861 caggcatcgg tcatgtagaa ggcaatgcta aactggctca gcaaactaag agcagcgcgc
     1921 atccacttac ccaatgcggt tgagaaaata gccttcgatc gctttcatgt ggcgaaacaa
     1981 ccgggtgagg tggtggataa aacccgccag aatgaacatc cgcatcttcc tgttgaaagt
     2041 cgtcgtcagg caaagggaac tcgcttcctg tggcagcaca gcgataaatg gatgaccgaa
     2101 tccaggcagg aaaagctgat atggctgcgt gc
//
LOCUS       DAZOYF010000333         2121 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZOYF010000333 DAZOYF010000000
VERSION     DAZOYF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.341"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..124)
                     /locus_tag="ACHUOF_004358"
     CDS             complement(<1..124)
                     /locus_tag="ACHUOF_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFR0613251.1"
                     /translation="MVVGCSSKENALCHPQPKPPAPPAWAMMPPSNSLQLLDETF"
     gene            complement(69..386)
                     /locus_tag="ACHUOF_004359"
                     /pseudo
     CDS             complement(69..386)
                     /locus_tag="ACHUOF_004359"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            complement(383..916)
                     /locus_tag="ACHUOF_004360"
     CDS             complement(383..916)
                     /locus_tag="ACHUOF_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0613252.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(982..1611)
                     /locus_tag="ACHUOF_004361"
     CDS             complement(982..1611)
                     /locus_tag="ACHUOF_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613253.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1615..1830)
                     /locus_tag="ACHUOF_004362"
     CDS             complement(1615..1830)
                     /locus_tag="ACHUOF_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR0613254.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          544 a          562 c          467 g          548 t
ORIGIN      
        1 agaacgtttc gtccagcaac tggagagagt tcgacggtgg catcattgcc catgccggag
       61 gcgctggagg tttcggctga ggatggcaca gggcattttc ctttgacgag cacccgacca
      121 ccattatcaa gcttgcgccg aagagcatca ttttcagctt tcgcatcagc taactccttc
      181 gtgtatttag catcgagtgc atcagcatca cgctggcgct gctgcatgtc agtaatggtt
      241 gcgttcgcca gcttcagttc tccggcgttt ttgtcgcgct gctctttgta ggtaatggcg
      301 ttatcacggt aatgattaac agcccatgac aggcagacga tgatgcagat aaccagagcg
      361 gagataatcg cggtgactct gctcattgat ctatccccca acaggctaat gcgctttcct
      421 ggtcacgacg aataacctgt ccatagcagt tatttgaacg tatgcggcaa tcgcgcccaa
      481 catcttttat ccaccagcga atcgcctcgc atgcgccctt acgatcaccg gcattcagcc
      541 gcttataaaa cgtcgacggg aaacacttac cggggccaat gttatagggg caaaatgacg
      601 ctatacccgc tttctggggt tcggtcagag gtactttaat attgcgctcc acccatgcca
      661 gcgccttatc acgttcaata gcgttaacct ggtcacattt ttccttcgac agcttcattc
      721 ccggtatgac aggcttacca tccaccattg tggcaccacg acagatggtc catatgccag
      781 aaccatcacg gtatgccgtt gtgtgattac cctctttttc gttcagaaac tggtcaagta
      841 tctggggagc agacgcgcct gcggcaatca gcgccagaac agcagccgac aggccgtatt
      901 tgatttttgc gctcatggat atttatcagg atttatcggc aacaaataac gagccaactt
      961 atatacgcct tttaagataa gtcagccccg gctggatcca gtcatcaggc tctttcttaa
     1021 agggggagta ttgaaatcac gaagaagagc ctcccgcacc gcagcatcca tgtctgcacc
     1081 actggcaagt gcttcaatct ctgccgccac ctgcaaatac cccatgcaac ggccaatgcg
     1141 tttcataact ctggtgtttt cgtctttcag gcatctgata gcgagattaa taattttcag
     1201 gcagtgatga tgcgatgccc ggacaggttt tccgttctca taaacgacga actcaccctc
     1261 ttcacaggaa accctgaaag gccgatgggg ttcctggtct ttatccggtg cagacgcaat
     1321 aaaatcactt ttatgctctt cctctccggc actgttaacc gtaatgacgt actcaggccg
     1381 caggtaagcc gcctcttttt ctgacaatgc ggccggtaaa aacttttcct gcaaaaactt
     1441 cataaaagtc tcgtgagcga ctttgtggca gtaatcgtaa atcgccgcat aatgctcatc
     1501 gcggcgtttg ttttcatctt cagaactcac caacgccgac aattttttat taagctcagt
     1561 gatttcattt tccaggtgat tgaatcgctg attcatttct tcatgcttca ttatttactc
     1621 tccccgtgca gccttacgct tgtcttctct tattttgaaa tacagattcg tcagataagt
     1681 cagaagcccc agaagcagac ttcccagcac accaatcgca gcccactgtg atggcctgac
     1741 ctgatccaac cactgcaaaa accagtagcc ggcactgccg gcggaggtgc cgtaggcaat
     1801 gcccgttgaa attttgtcca tggatttcat agcctcacct ccgcaaataa cggatggcgt
     1861 agttttacac tgagaaatga aagggatttg aaaagaaaaa aacgcaaaag cgggcgaaac
     1921 gatatataca gtaaggaaag cactctatcc aacaaaccac ccacagttaa tcggaataaa
     1981 agcagagtgc ttatgaatga tcgcacgccc gaaggttagt atttctgcac agcaattttg
     2041 caaaaaaagg cgatcattca taacttaaac gtctttcagt cactccggga tttcccatca
     2101 tcgcagactg aaagactctg a
//
LOCUS       DAZOYF010000334         2117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZOYF010000334 DAZOYF010000000
VERSION     DAZOYF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.343"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            29..979
                     /gene="virK"
                     /locus_tag="ACHUOF_004363"
     CDS             29..979
                     /gene="virK"
                     /locus_tag="ACHUOF_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR0613255.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            1044..1988
                     /gene="lpxM"
                     /locus_tag="ACHUOF_004364"
                     /gene_synonym="msbB"
     CDS             1044..1988
                     /gene="lpxM"
                     /locus_tag="ACHUOF_004364"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0613256.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT          599 a          406 c          484 g          628 t
ORIGIN      
        1 ctcacattag cgaagcacaa gtaacaatat gttttctgta agtaacttat catttatcgg
       61 tttccttaaa aggattgttt tttcctcaga ttcactcccg gggaagtggg aacacagaaa
      121 attccggttc atgtacattt tgcgatgtgc tataaatccg gttgccagta ttcgatatta
      181 ttacgaactg cgttccttgc agtgcattga ggatattctg gcaatacaac ccacgttgcc
      241 agcaagaatt catcgacctt atttgcataa aggggggcga gcctggtccc gggggcaata
      301 tattcttgag cattatcgct ttgtgcaaaa tttgccagaa aaatattctg aatttttgtt
      361 tccacaaaaa tcggtatcat tagtccaatt catcgggaaa gatggagagg attttgatat
      421 ccagtgttct cccagtggtt ttgaccggga aggagagttg atgttgtcat tgtttttcaa
      481 taaaatagtg attgcccggt taacattttc agtcattctg actcaaaatg gacacaccgc
      541 ttttattggc ggattacagg gagcccccaa aaataccgga cctgatatta ttcggtgcgc
      601 aacccgggcc tgttatgggc ttttcccaaa acggattatt tttgaagcat tttgtgcgtt
      661 gatgaaagct tgtaatgtct ctgagtgtct ggcagtcagt gagcacagtc atgttttccg
      721 gcaattgaga tactggtatc agaaacgcaa aacctttgtt gctgtctaca gtgatttctg
      781 ggagtctgta gcagggaaaa cctgtggtga ctggtacaaa ttaccaacac aggtggttcg
      841 aaagccgcta agcaatatag ctagtaaaaa acgctctgag tatcgaaaac gatacgcttt
      901 gctggattat atccatgaaa ctgctatccg ctctttggat gcatatcctg taaactcaga
      961 acatcaggac ttaaattaag gtttgatgta ttctctgaat aaaatattaa tgatgatttt
     1021 ggtaggggca ttcgcactaa ataatgaaaa aatacaaatc cgagtttatt cctgaattta
     1081 agaaaaatta tctttcccct gtttactggt ttacatggtt cgttttggga atgattgcag
     1141 gtatttcaat gtttccccct tcattcagag atcctgtctt ggccaaaata gggcgttggg
     1201 tgggcagatt gagcagaaaa gctcgtcgca gggcgacgat taatttatcg ctttgtttcc
     1261 cggaaaagag tgatacagaa cgggaaataa ttgtcgacaa tatgtttgcc acagcattgc
     1321 aatccatagt gatgatggca gaactggcaa ttcgtggtcc ggaaaagttc cagaaacgag
     1381 tgttctggaa aggcctcgaa attcttgagg aaattcggca caataacaga aatgtgattt
     1441 ttctggttcc ccatggctgg agcgtggata ttcctgcaat gttgctggca gctcaggggg
     1501 aaaaaatggc cgccatgttt catcagcaac gaaatccagt gattgattat gtctggaatt
     1561 ctgtacggcg taaattcggg gggcgcttac attcccggga ggatgggata aaaccattta
     1621 ttcagtcagt acgccaggga tactgggggt attaccttcc agatcaggat catggtcctg
     1681 aatacagtga atttgctgat ttttttgcga cctataaagc gacattacca attattggac
     1741 gtctgatgaa catcagtcag gctatgatta taccgctttt cccggtttat gatgaaaaaa
     1801 aacatttcct gactattgaa gttcggccac caatggatgc atgcattgcc agcgcggaca
     1861 ataaaatgat tgcccgacaa atgaacaaaa cagtggaaat tttggtgggg tcacatccgg
     1921 aacagtatat ctgggtttta aaattgttaa aaacgcgcaa atcaaacgaa gcggacccgt
     1981 acccttgaaa ttataaagta caggtatttc cactagttgt ttcttacagg ttaccaatcg
     2041 aaacacatcc cccttccgtg tgttgcccgt ttggcacgga ggactgtgta gatgcagttc
     2101 gttaaattta atttatg
//
LOCUS       DAZOYF010000335         2092 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZOYF010000335 DAZOYF010000000
VERSION     DAZOYF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.344"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="ACHUOF_004365"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="ACHUOF_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR0613257.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHUOF_004366"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHUOF_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR0613258.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          559 g          512 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tt
//
LOCUS       DAZOYF010000336         2089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZOYF010000336 DAZOYF010000000
VERSION     DAZOYF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.345"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHUOF_004367"
     CDS             complement(60..1025)
                     /locus_tag="ACHUOF_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR0613259.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQIAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHUOF_004368"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHUOF_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613260.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          578 a          549 c          436 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcaatc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag acttgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcactccc gaacggctga acccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZOYF010000337         2085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZOYF010000337 DAZOYF010000000
VERSION     DAZOYF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.346"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..797
                     /locus_tag="ACHUOF_004369"
                     /pseudo
     CDS             1..797
                     /locus_tag="ACHUOF_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            912..1253
                     /locus_tag="ACHUOF_004370"
                     /pseudo
     CDS             912..1253
                     /locus_tag="ACHUOF_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1236..2073
                     /locus_tag="ACHUOF_004371"
                     /pseudo
     CDS             1236..2073
                     /locus_tag="ACHUOF_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          504 c          631 g          435 t
ORIGIN      
        1 tgggccttgc ccgtagcagt tatttttatc accgggctgc actgaaagcc ggtgataaat
       61 acgccacgat acgtaagatg ctgacagata tctttaacag taattaccag tgttatggct
      121 atcgtcgcct gcatgcgatg ctcaggcatg agggggggcg gctatcagaa aaggttgtac
      181 gcagacttat ggtggaggaa cagcttgtcg tcagtcgtaa ccgtcgtcgt cgctacagct
      241 catattgcgg agaaatcgga ccggctccgg ataaccttat cgccagagat tttaaggcgg
      301 agcaacctaa tcagaaatag ctgacagata tcacggagtt ccagctccct gcaggtaaag
      361 tctggctgtc accagtggtg gactgcttcg atggaaaagt tgtgagctgg tctctcagta
      421 cacgccctga tgctgaactg gtcaacacta tgctggatag cgctgtcgaa acgttaaatg
      481 ctggcgaacg accggtgata cacagtgaca gaggtgggca ttatcgctgg ccaggctggc
      541 tggaaagagt gaatgcagca ggtcttattc gctcaatgtc ccgtaaagga tgttcacctg
      601 ataatgctgc atgcgaaggc tttttcggca gactgaaaac ggaaatgtat tatgggcgta
      661 aatggtcggg catcacgcca gaaaagttca tgcagcaagt ggatgcttac atcagatggt
      721 ataacgagcg gcgtataaaa ttatcgctgg gtgccgtcag ccccaaaatg taccgccaac
      781 aatgcgggct ggaatgataa agcagtccag gaaatcgtcc gcatcccccc ttcaccattt
      841 ggattgtccg ctccacccaa catgttgttt ccttaaggtt cctacaccac aaaggaaact
      901 ctcatgctgc gcagtggatg gctgacgttg ccgacaggcg ggaacttcgc cagttcaggc
      961 agacaccgga acagcgcttc acgcaggaac aggagcatct gcagccgctg ctgggtacgg
     1021 acttcgatat ccgtcatgtc tcctgggatg gctatatcga ggttggcggg aaccgttaca
     1081 gtgtgccgga aagcctgtgt ggtcagctgg tatcaatacg gatctcgctg gatgatgaac
     1141 tgcggatcta cagtaatgag caacaggtag catcgcaccg tctctgttcg gctgcatacg
     1201 gctggcagac cgtgccggag catcacgctc cactctggca gcaggcttcg gagcgcatgg
     1261 ccgaatggct gggggagata cagaaacggg taatcactgt ctgtgaccgg gaagcggata
     1321 tctggcatta cctgtattat aaagtcagcc acggacagcg tggtgcgtgc tgcacagaat
     1381 cgccggctgg aagaggcacc cggcaagctc ttcgaactgc cggaagtcct ggcaaccgca
     1441 ggaagccaca cgctgaatgt gatgcaaaaa ggcgggcggg cagcccgtca ggcccggatg
     1501 ttcatcagtt acagcgaagt cagcataaaa aatcccgaca acagcggcca ggcgctcccg
     1561 ctcacgtatg tctgttgccg ggagcaggca gaggacggtg cctgctggca tctgctgacg
     1621 tcaggaaaag cggcgagtgc cgccgatgca cgacgtattg tcagccatta cgagcgacgc
     1681 tggctgacag aggaatacca caaggcgtgg aaaagtggtg gtacatggaa tcgctgcgaa
     1741 tgcagacccg ggataacctt gagcgcatgg tggttatcct ggcgtttata gcggtacggg
     1801 tgctgggcct gcggcaggga ggtgtcagtg aagaaacaca aaatgacagc tgtgagaaaa
     1861 tactgacgcc gacagagtgg aaactactgt gggttaagct ggaaggaaag ccgcttccgg
     1921 tgcaggcacc gaccctgaaa tgggcctggg gcgatggcat gacagcaaac gcacaggtcg
     1981 tcccggttgg ggcgtcatgt gggatggctg gtcagacttc aggatatggt tgagggttac
     2041 ctggtgatga agtctcttga tcaggagctc tgatcaagag acagc
//
LOCUS       DAZOYF010000338         2071 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZOYF010000338 DAZOYF010000000
VERSION     DAZOYF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.347"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..316)
                     /locus_tag="ACHUOF_004372"
     CDS             complement(<1..316)
                     /locus_tag="ACHUOF_004372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613261.1"
                     /translation="MACDYRYKNRQYHCLSGSYMARSAKPRKRKPAPQRSKLLRYVVK
                     LHEDDFFDEEEAEVLRFDNFDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEI
                     ARC"
     gene            complement(1012..1410)
                     /locus_tag="ACHUOF_004373"
                     /pseudo
     CDS             complement(1012..1410)
                     /locus_tag="ACHUOF_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
     gene            complement(1413..1779)
                     /locus_tag="ACHUOF_004374"
                     /pseudo
     CDS             complement(1413..1779)
                     /locus_tag="ACHUOF_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1781..2071)
                     /locus_tag="ACHUOF_004375"
                     /pseudo
     CDS             complement(1781..2071)
                     /locus_tag="ACHUOF_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          472 a          581 c          488 g          530 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt
      181 gaagttttac gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg
      241 gtttagctga acgggccata taactacctg aaagacaatg atattgtctg tttttataac
      301 ggtaatcaca ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa
      361 cgcttattta gtaccagatc ttacttataa taaaggggct tggcagtttg gataaactat
      421 tgtccttatt ccactaatac aaactttttg taaagtatat taaatatact tcatttcata
      481 ctgaaatccc cggaagtatt caatcaaaca attacaccat aaaaggaaga gggggcccca
      541 attaagttgt caagcatgtt tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca
      601 ttggtactct ggcgctcatg gggcttccac ttgcgcttct cccttcacat cggggtgaca
      661 ggtccacgtt ccccacaaga gcctgaatcg acttcacgcc gcttttatgc cggatgccgc
      721 gcaggcagta aacatatccc acaggaacga ctccaccatt gttttgacat catctatatg
      781 ctttcgacac atcatcagcg gttcactttc gttcgtctct tcgaaccata cctgatcggc
      841 tcacgtccga ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg
      901 atggtttggt gcctgcgcct gcacgccgac accaaggggc cttccctcat ctcttgtgct
      961 gcatggctgc acagggactc acgtccccag gacgcgcctt cgtggcacac agtcatcggc
     1021 atagcaacag taagcaacgg caggtttcca ttgccagttt tctttgacag cagtacttcg
     1081 gcctcgctgg atactgtgat tccagtacca gcgatccttt ctggcttttc cactcaggta
     1141 gcgtttattc agatactggt cgaactcatt cagtatgatg ttcgacagca acggcgagat
     1201 cacaccgccc tgtggcatac tttcactgac cgcccggaag agaccaacat caacacggcg
     1261 ttttccacag cagtctcata aaacgtgtat cactgatcct gcggcagacg gctttcatca
     1321 gcagccggtg atgtactgtg tcgaagtagc tggacaggtc accttcaata acccggcgcc
     1381 ccgtgtttcc ccgcagtcgg tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg
     1441 ttcgggccac tggcgtaaaa atcctgcgtc agcagatttt ctgatacagg caggccgtgt
     1501 gcacggtaac tgatcgggct gaacttctgg gaggcttttg cgctcagttc ctgacggcgc
     1561 agactggctg ccacggtttt tacgttaaag tggtaaccag agcacacagt tcatccgtca
     1621 ggcgtggggc accgtaacgc tggactgggt aaaagccttg cggacaacgc tgtcgcagtg
     1681 ctgacggaac tgcggacgtg tgtttatcct tgtccgccgc tgacaccacg tataccagcc
     1741 gctgcgggcc acccggagta cgcggcacat tgccttgatg acggtttacg cggtcgcctg
     1801 cggcaacggc tttccggctg acggtagtgt ggaccttttt accccggaga acaccggcaa
     1861 gccccataac cgccatgaga cgtgccacag tgcatctggc cactctgata ccttcccgta
     1921 acaactgatg ccagacttta cgcacagcgt atacctgatg attctcatcg tatacgcgca
     1981 gtatctcttt cttcagccag tcatcgcgct gcgcacgggc actgcgttta tcatgatgat
     2041 gtcgctgttg ctgacagtgg taatacgttg a
//
LOCUS       DAZOYF010000339         2068 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZOYF010000339 DAZOYF010000000
VERSION     DAZOYF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.348"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..491)
                     /locus_tag="ACHUOF_004376"
     CDS             complement(<1..491)
                     /locus_tag="ACHUOF_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR0613262.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAH"
     gene            complement(504..1421)
                     /locus_tag="ACHUOF_004377"
     CDS             complement(504..1421)
                     /locus_tag="ACHUOF_004377"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR0613263.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            complement(1418..1780)
                     /locus_tag="ACHUOF_004378"
     CDS             complement(1418..1780)
                     /locus_tag="ACHUOF_004378"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HFR0613264.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT          641 a          426 c          387 g          614 t
ORIGIN      
        1 tcatgtgctt tccataattt tgacttggat tcttcagata ttattatcat tgcaacatac
       61 aatgataaat accagtgagc atttgttata ttggcttgga cttcatcaag cccgggcatg
      121 caaattaaat atacagagat gaagattttt gatgtggtgc ttaggaattt aaatctatcg
      181 gtaaataaga accatataat tatagctcta attattattc caaaaaagtt tgataataat
      241 ggtgcgtaaa ttggattaat aaatgtcgtc gcaccaacaa tgagtgttga tactgtttga
      301 aaataaccat ttcttggggt tatgagtgaa ctgaaaaaac cttgttcgta tgcatcttta
      361 taccaaaaaa ctgcatcctc agcccaaaac tgggcatttg tcaaaatgtc aatgcgtcta
      421 agccaaatca aagtaaatgc aagtataaaa attgatatct tgtgccaatt gcgcattatg
      481 aatatcttca taagatcatc ctgttatttt ctgcttttaa ttaagtaccg tggtcttgat
      541 tttgtttcta aataaattct acctatatat tcaccgagaa caccaatccc gatcagttgc
      601 actccaccca ggaaaagtat tgatacaagc atggagggat acccgcgtac tgggttacca
      661 aagacaatgg tgtctataat catccatgca ccatataaaa atgaaatgct tgcaacaaac
      721 aagcctatat aagtccatac gcgaagagga aaggttgaaa aacttgtgat accttccagt
      781 gccagattcc ataatttcca gccattaaat tttgagatgc cagcaacacg ctctgtgcgt
      841 acatattcaa cgacatctgt ctgaccacca acccagctca gtatgccttt catgaaaaga
      901 ttgcgctcag gcaatagttt aatgttctcc acaacctcac gagacatgag tcgaaaatct
      961 ccgacatttt cctctatttt tggggtgctt attttgttgt gtaatttata gaaccactca
     1021 gctgtcttac gtttcagccg tccatcagtt gagcggtctg aacgtttagc aagcaccatg
     1081 tcagcacctg cctgccactt ttcaataaga tgagggataa cctcaatcgg gtcttgcagg
     1141 tcaacatcaa ttggaatcac ggcatcgccg gttgtatggt ctaaccctgc aaacaatgct
     1201 ggttctttac caaagttgcg tgtaaatgac agcggaacaa ctagcggatc agaaacagcc
     1261 agagcgttaa tgattgactc cgtagcgtct ttgcttccgt catttatgaa aacaatttct
     1321 acttcatatg gcttcaactc ttcgaattca cgtaccgttt tataaaaaat tggtatcgct
     1381 tcttcttcat tgaagacagg aacgacaaga gatatcttca tttcgcatcc ctaaagacaa
     1441 tgaactttga atagacgaaa ccgcacacca agctgatggc ggagaaggta acaagagtga
     1501 caattggagg aagtgaacat ctatcagcaa cccatccaac agtagcactc agtgttccca
     1561 tgaacccgac atataacatg tagcgcatcg ttgtagttga tgctttgaat gtgaattttg
     1621 cattcgcaaa gaagctaaaa ctcacagcca caacgaaacc tgcgaagttt gccatagcct
     1681 gattggtatg cgcggcatag atacatacac caaaaaccac ccagtgtata agtgtgttca
     1741 gcacgcctat agatgtgtac tttgcaaata gctttaacat ttcttctatc agctaataat
     1801 caaaggcatg aagtctatca tccaagtctc aatcgatcga tacttgcggt agttgatgag
     1861 aaaaactctg gcacacaaat ctttgcactg gattgcaagg ctttgtgctc ttctgtgaat
     1921 gtgtggctat atgtttgaag atcgttgtgc cgtatttgtg acatatatat ggcaacatca
     1981 ttcatcaact ttctgtttgt gccatcaact atatcttagt gaatgcggtt aatgcttgct
     2041 aaaacagata gttatgattg gtgctaca
//
LOCUS       DAZOYF010000340         2048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZOYF010000340 DAZOYF010000000
VERSION     DAZOYF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.349"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            16..1098
                     /locus_tag="ACHUOF_004379"
     CDS             16..1098
                     /locus_tag="ACHUOF_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR0613265.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(1587..2048)
                     /locus_tag="ACHUOF_004380"
                     /pseudo
     CDS             complement(1587..2048)
                     /locus_tag="ACHUOF_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          591 a          432 c          417 g          608 t
ORIGIN      
        1 ataaaagggc tcacgatgaa aaaattaaca gtggcaattt ctgctgtagc tgcatcagta
       61 ctgatggcga tgtctgctca ggcagcagag atttacaata aagacagtaa caagctggat
      121 ctgtacggaa aagttaatgc taagcactac ttctcttcta acgatgcaga tgatggcgac
      181 acaacttatg ttcgtctggg cttcaaaggc gaaactcaaa tcaacgatca actgactggt
      241 ttcggtcagt gggaatatga attcaaaggc aaccgtgctg aatctcaagg ttcttccaaa
      301 gataaaaccc gtcttgcctt cgctggcctg aaattcggtg actacggctc catcgattat
      361 ggccgtaact acggtgtagc atacgatatc ggtgcatgga ccgacgttct gccagaattc
      421 ggtggcgata cctggaccca aacagatgtg ttcatgactg gtcgcaccac aggtgttgca
      481 acctatcgta acaacgactt ctttggtctg gttgatggtc tgaactttgc tgctcagtat
      541 cagggtaaaa atgaccgcac tgacgtaact gaagctaacg gtgatggttt cggtttctcc
      601 actacttatg agtatgaagg attcggtgta ggtgcaacct atgctaaatc tgaccgcact
      661 aatgatcagg ttatctacgg taacaacagc ctgaatgcat ctggtcaaaa tgctgaagta
      721 tgggcagctg gtctgaaata tgatgcgaac aacatctatc tggctaccac ctattctgaa
      781 acccagaaca tgactgtttt tggtaataac catattgcca acaaagcaca aaacttcgaa
      841 gtagttgcac aatatcagtt cgacttcggt ctgcgtccgt ccgttgctta cctgcaatct
      901 aaaggaaaag acttgggtgc gtggggtgat caggacctga ttgaatatat tgatgtaggt
      961 gcaacctatt acttcaacaa aaatatgtcc acttttgttg attacaaaat caacctgatt
     1021 gataagagcg atttcacgaa agcatctggc gttgctaccg atgatatcgt tgctgtagga
     1081 ttggtttacc agttctaatc tgattactca aagatatgtt gtgggaggct ttgcctcccc
     1141 aacatataag tggcgctctc aagccacttt ccttagaagc actatcttgc ttctgactat
     1201 ataaattacc ctttattttc tgggacgttt caacgcccca tttttaataa tactcagtga
     1261 acaattaacg tatcaattag atttaataac aacgatatcc gtacctaacc tgatatctaa
     1321 tgtcataagc atccctttaa ttatcccctc aacctcttgt aaatttccca ctcttgagct
     1381 ccacatagcc caaacgccgg atgcaaaaaa aatcatacgt gcatctctga aattgtgatg
     1441 gaggaagtga gtatcaatta tatgtgctta taaacgttcc cgattggcac agaagtcacc
     1501 ggattgttca ggctccaata ggttgagtat gtcgtattta ttttccaaaa taaaaatcga
     1561 tgctgttgct ccttaataaa atacttttac tctctggacg cttttcttag ctctcttggt
     1621 gttatattaa aacgattatg gaacctttca gtaaaacgag aaggacactt gtaaccattt
     1681 tccctggcaa tctcgctgat gggttttact gtcgtttgta tagcaggaca acgcattatt
     1741 taacctcacg tcgtccaata tactttgaaa acttattccc tcgcttgcca gacggcgatg
     1801 caatgtggaa acagaaatgt agagatatct cgctaccttg tttgctgtcc attttgttct
     1861 aggttcggat agcattaggt tataacaacg acttattaat gattgtttac tatctgataa
     1921 aagtaaacaa ttaacatgat gtgctcctaa tgaaagtaga acgcccattg ctaagtgttc
     1981 ctgaatttgc gttgagaagc ctcgggaaat agatgttttt agttgctccc aacaatatat
     2041 taactcgg
//
LOCUS       DAZOYF010000341         2042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZOYF010000341 DAZOYF010000000
VERSION     DAZOYF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.350"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(53..322)
                     /locus_tag="ACHUOF_004381"
     CDS             complement(53..322)
                     /locus_tag="ACHUOF_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0613266.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            379..1047
                     /locus_tag="ACHUOF_004382"
     CDS             379..1047
                     /locus_tag="ACHUOF_004382"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0613267.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1363..>2042
                     /locus_tag="ACHUOF_004383"
     CDS             1363..>2042
                     /locus_tag="ACHUOF_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0613268.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          613 a          446 c          381 g          602 t
ORIGIN      
        1 gccaaccgca gcacgtcctg catacgtcgt gtctgcggtt tttctttttc gcttacattg
       61 tgtctggttc ttccggccac tcaatatcag gtgcagttga tgtatcaaca cggttcagca
      121 acacccgata cttcttccag gcttccagca acgagttttc ttcctccgtt gcgatctcca
      181 gatctacagc atcctgaagt ggcgcaatat gctcactgaa tccctggatg tagaactgtg
      241 tggtgacggt cttccagcca ttcggctcct gctgtatcga agcataccag gctatttcaa
      301 tatcgctatg ctgcggcagc atttaacccc ttgtaattca tcgccataat tgatttaatt
      361 cacaaataaa actataatat ggtgaaatta atgaaaaaaa acacagatga tggggctaaa
      421 atttacacac cacttaccct aaagctttat gactggtggg ttttgggagt atcaaatcgg
      481 cttgcatggg gatgtcctac aaaggaacac cttcttccac actttctgga acatttaggt
      541 aacaaccatc tggatattgg tgttggaact gggttttacc ttactcacgt acctgagagt
      601 agtctgatat ctttaatgga tttgaacgaa gctagcctga acgcggcatc tacaagggct
      661 ggggaatcaa aaattaaaca taaaattagc catgatgttt ttgaacctta tcctgcggcg
      721 ttacatggtc aatttgattc catttccatg ttttaccttc ttcactgcct gcctggaaat
      781 atatctacaa aaagctgtgt aatacgcaat gcggcgcagg ccttaactga tgatggaact
      841 ctatacggag ccacaattct tggcgatgga gttgtgcaca atagcttcgg tcaaaaactg
      901 atgcgcattt acaatcagaa aggcatcttt tcaaacacaa aagattccga agaaggctta
      961 acacatatac tctcagagca tttcgagaat gttaaaacca aggttcaagg tactgtagta
     1021 atgttttccg cttcagggaa aaaatagcat ccaaccgcag cacgttcttg cttaagacgt
     1081 gctacggttt cctttacgat ttggggttgc gactttacct ctattgataa tgcattccgg
     1141 cagaacgttc aaatatgaac gtacgatatt taactaaccg aaaaacaaaa tatagcacag
     1201 gcgagacatt taccatctaa gaaaatagta tcgttttttt acagttaaat caacatcact
     1261 tccttaaaat gaaaacaata aatttcaatc ctgaaacatt ttatttcagc ttattattcc
     1321 ccagtatcac attttatcag gatatccttc tgcgaggtta ttatgcttcc tgtaaataat
     1381 ccccccctat ccactggaaa cgtctctttt tacagaacta catcaatcga caatgttcac
     1441 aataattatc tctccgaatg ggttgaatgg actaaaaaca gcatttccgg agaaaacagg
     1501 gaaactgctt ttacccggct ccaattatgt ctggagaaca gtgaaacatc gttggactta
     1561 tcttgtttag gtctcagatc tctaccacga ttgcctgaca atcttgatga aattaatgta
     1621 agcaataacc aactatcaat gctccccgag ctaccaaggg cattgaaaga gctgaatgca
     1681 agcagtaatc aattatctgc acttcctgaa ttaccagtgt cgctggaata tataaatgtg
     1741 agtgataacc atttgttcgc acttcctgaa ttacctgcgt cactagaata tattaatgta
     1801 agtgacaatc acctgtctgt acttccgagg ttaccaatgt cattggaatt acttgatgca
     1861 gccagaaatg ctttggaagt aataccagat tttccagaaa gagatgatca tattataaga
     1921 atattctggc ttaatcagaa ccggatcacg gcaattccgg aaagcatact tggcctcagt
     1981 tctgatagcg ttgtcaatct tagagaaaat caactatctc ccagaataat gcaaactttg
     2041 tt
//
LOCUS       DAZOYF010000342         2041 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZOYF010000342 DAZOYF010000000
VERSION     DAZOYF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.351"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(13..901)
                     /locus_tag="ACHUOF_004384"
                     /pseudo
     CDS             complement(13..901)
                     /locus_tag="ACHUOF_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          551 a          476 c          474 g          540 t
ORIGIN      
        1 gtattaagac tatcacttaa ataagtgata ctggttgtct ggagattcag ggggccagtc
       61 tagcagctgc tctacggtca tatttttaat tgcgctccgg tttacaagag cccacccctg
      121 tttttccaga taaaactgga aagtatccag ggtacagacc agtgcaccgt caggaacagt
      181 ttcggtgaat ctgatattgc cgtgttcgtc gaagtgcaca accagagtgc gaccgttacc
      241 cggaatcatc ttgtcagcgg acggggtatt attctggtgc agttctgcct ccatgcggtc
      301 gaacatcagc gatgtaggcc tctttgaatg cagcggcttt tttcccagtg aagcccatca
      361 ccaggaaaac gaagccgttt ttggtgattt ggtacattgg gcgtttttcg cctttggtgt
      421 cggtgtaggt gacgtcctca aaattgaggg cgttaaattc aggtgagcat tcagtatttg
      481 cgatggcacg taacacattg tcgtgtcgtt tgtggaagaa ctctgcaacc gcaacagacg
      541 tagtgacagc gcgaccgttt tcgatggtta cgtcagggtg agaaagggca gggatagtag
      601 ccatgatggc atcctcgagt gataagttaa ttaactcacc accgaggttt tccacgacca
      661 taagggtggt gagacgtaca ggggtggaaa taccggtcac tcgagaaccc ggccagtctt
      721 gcgactgccc tgcacatccc accataattt gaatggagct gtgcattacg cataaaaaaa
      781 ccgcttcagc gcggctatgc gctcgagtaa cgttcgggtt tccacgcccg gcccccgttt
      841 tatgaggtgc agatgcacta taattccacc cgttctggtt ttcaatagct acattcaaca
      901 ttttctctta cctttcatca ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag
      961 gtgacgttaa ccagttaaca attaacgccg gatacaggga aacccataac cacccgatac
     1021 gtgggaacta atcgcttttt aactgttccg cgtgcgctca gccgcattca ccgcatcaca
     1081 aaattcactt taaaaagggc ggacatcagc cagcaatgaa actgatgccg ccaaaggtac
     1141 caatcaacat ggagtgttgt ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc
     1201 cggtgtcctc aacggggaag gaataactcc gccatactta ccgccgcgtc atttcgcgtt
     1261 gtgtgcctgc ttttaactac gtcaggcgag gtggttcctg ttattcccca acaacaagaa
     1321 atttgtataa tctgaatacc ccaacaataa gaagagtgag caagtgatgt attagcgatg
     1381 tcagctctca aagagactgc atcaatgtta aaattattca atgaagcaaa aactcaggct
     1441 gaggttgatc gcgctgtgtc tgaaataaac tctaagttgt caagcattca gcttgaatat
     1501 ttgtcatctc attgatgagc tgacaatctc taaaagcaat gaggctgcgc tcaaagcaaa
     1561 actcacggag atagaaaagt ttaagttcca aagtgaagac tgcgaacctc gctaattgat
     1621 tactggaacg attgtgtatg ccagacagtg caatactagc tctggcaaga ttaccatgta
     1681 tttttgtcca gaatgttttg cgaagagagc aatatctata ttgcaacctt tccctatcga
     1741 ggaaggcgat aaatatacga aaactcaatg cccctcctgt aaaaatgagt atctcatgga
     1801 ctacaatttc aataatgatt tttgagtcac ggtattcaga tttttaaaga acatgccgga
     1861 tactcacccg tgtccggcgc acgcactcca cctcacccgt ggagaactcc ttaattacca
     1921 accttggctt cgttggttag ccattaacgc tggtatgtaa tcattctggc aatgcttaat
     1981 ttgaagtggc acactgaatt tggccacctg aacagaggtg atatgctcac ctcagaacaa
     2041 c
//
LOCUS       DAZOYF010000343         2032 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZOYF010000343 DAZOYF010000000
VERSION     DAZOYF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.352"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1327..1929
                     /gene="fimB"
                     /locus_tag="ACHUOF_004385"
                     /pseudo
     CDS             1327..1929
                     /gene="fimB"
                     /locus_tag="ACHUOF_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
BASE COUNT          625 a          350 c          407 g          650 t
ORIGIN      
        1 tttttgtata aggtctcctg tgaaaattct cttttaacat tatttaaata aacagagatc
       61 cagattaaat acctgagtat aaaatctctt ctgattttta attgatttga atattcgtaa
      121 gttatatcac ttactcaacc cattttaccc agagtcataa aatagagtgt ctgttgatct
      181 cattttcata ttcctttcag actgaagtgc attttcatca cccgatgtga aagaactgaa
      241 gtgatgtttg ccatagatta cgagatgccc agtctcattg actatcgtgg tgcatcttta
      301 tacctgttat accagatcaa aaatcacgca atccatacaa caaaaccaga tttgcaattc
      361 gcgtcacaaa atatgtcgat ctttttctaa gaagaagatg ccatgtgaag ccagacgaac
      421 acttgcggtg gtcttcaaaa actaaagatt ttcttttaac tatacgtttt ataaataatt
      481 tatttagaat ctaaacaaag aaagctttgc aagctaactc agtgagagtg gtgaaaataa
      541 gggtttactc gccatcaggc tgagcataat tctcatcatg aaatatgttt cttggtttgt
      601 ggcttgtaac tggtcactcc tgatgtcgat ctggagaggc ttgttgatgt tggtgttttc
      661 aggatgatgt ttcacttagt ttgtttgccg tatcgcccgg cgaatagctg tgattgagga
      721 aggcttaagt cgtagtgacc aaagctatat ttaccaacgg atgtagatga aaaagcgtcg
      781 cctgcgttcc cccatatctc caggataaaa aggaatgtaa caatctcatg gcctaagctg
      841 acgaaccagg caggaataat cgctagggat ctaagaatta gcatgataat agccactaag
      901 aaattactgc gttccatgaa atagccattt tgtggcaaat gcagttaact aataatgtca
      961 tatttgagac ggctagttta acaaatatta aattttgctg aattttttat gttgatttta
     1021 cttgttacag aacatatcac atgatatata gataagatta gttgcattaa tgatgagggt
     1081 tattattaga ttcgtatcag attgataaat atataaaggt acatagcatg caagagcatg
     1141 gcgtttgtat ggcaaagtta ttataagtaa ctatcgctac tccatttaag ttcgctcaga
     1201 agaactggtc cacttacgta agttattaag caaacgttcg cttttataaa cataatcagg
     1261 ataaaaatgt tggattattg ctaacccagc acagctagtg cgcgtctgta attataaggg
     1321 aaaacgatga ggaataaggc tgataacaaa aaaaggaact tccttaccca tagtgaaatc
     1381 gagtcactcc ttaaagcagc aaataccggg cctcatgcaa cacgtaatta ttgtctgatt
     1441 ttgctttgtt ttattcatgg tttccgggcg agtgaaattt gtcgattgag gatttcggat
     1501 attgatctta aagcaaagtg tatatatatc catcgattaa aaaaaggctt ttcaacaacg
     1561 cacccgctat tgaacaaaga agttcaggct ttaaaaaact ggttgagtat ccgtacttct
     1621 tatccgcatg ctgagagcga gtgggtattt ttatcacgta aggggaatcc gctttctcgg
     1681 caacagtttt accatattat ctcgacttct ggtggtaatg ccgggttgtc actggagatt
     1741 catccgcaca tgttacgcta ttcgtgtggt tttgctttgg cgaatatggg aatagatacg
     1801 cgacttatct aggattatct tgggcatcgc aatattcgtc atactgtctg gtataccgcc
     1861 agcaatgcag ggcgttttta cggcatctgg gatagagcca gaggacgaca gcgtcatgct
     1921 gttttataga gatagttgag ataccaggga tggtgttaaa aactacatta ttcttacgat
     1981 atatcaaaaa tgattaacca tttattaatt gatgttattg taagtgtgat tc
//
LOCUS       DAZOYF010000344         2031 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZOYF010000344 DAZOYF010000000
VERSION     DAZOYF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.353"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..2031
                     /gene="sat"
                     /locus_tag="ACHUOF_004386"
                     /pseudo
     CDS             1..2031
                     /gene="sat"
                     /locus_tag="ACHUOF_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          745 a          356 c          401 g          529 t
ORIGIN      
        1 acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata ttcggagaca
       61 gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta gtcggaacaa
      121 cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa tacaatgata
      181 aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat ggcaataatg
      241 taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc actggtactc
      301 aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaagt gttctgttta
      361 aggataattt ggattttggt tgcggtggta ttatctttga cgaaggccat gaatataaca
      421 taaacggtca gagatttaca tttaaaggag caggaattga tatcggaaaa gaaagcattg
      481 taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc cccggtactc
      541 tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat gttattctta
      601 atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag gtaatactaa
      661 ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact aaacgtggtg
      721 gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc actgacgatg
      781 gaacaacaat aactaactca gatacaaaga aagaagccgt tctggcaatc aataacgaag
      841 actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac aatattaatt
      901 ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac acaaaaaatg
      961 atgttgaagt cagtaatgcc agtcttacca tgtaaggcca tgcaacagag catgcaatat
     1021 tcagaagcac agcgagtcat tgctccctgg tatttctttg tggaacggac tgggtcaccg
     1081 ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac aaaagtaata
     1141 atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa tttgatacat
     1201 tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa ggaaatatat
     1261 cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat aatcttgcag
     1321 ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact aatctatcca
     1381 taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata gccataggtg
     1441 atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct ataagtatag
     1501 ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt attgatatct
     1561 ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta acgccgggtc
     1621 tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc attgccagaa
     1681 atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt actctgggac
     1741 agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca gagactcttt
     1801 tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct acagttagca
     1861 tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta gaaacaaaag
     1921 acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca gtggacaacc
     1981 ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca g
//
LOCUS       DAZOYF010000345         2031 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZOYF010000345 DAZOYF010000000
VERSION     DAZOYF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.354"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..362)
                     /locus_tag="ACHUOF_004387"
     CDS             complement(<1..362)
                     /locus_tag="ACHUOF_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0613269.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHUOF_004388"
     CDS             complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHUOF_004388"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFR0613270.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          632 a          492 c          413 g          494 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aataccctca aaaaccacct gaataattaa
     1801 ccattattgt taaaacaaca cgttgaataa tcacataaca aactccatta atcattgaaa
     1861 atgagcacag aaatagaaat taattatatt caattaatta aaatgtgtat tgcttgatgt
     1921 atttaaaaat attattttca tggtgagagt ggaggtaaag aagacacaat aataaaaagg
     1981 aatgtgacaa ataatggttc tggttgggcg ttaaatttaa ggtcaccaga a
//
LOCUS       DAZOYF010000346         2029 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZOYF010000346 DAZOYF010000000
VERSION     DAZOYF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.355"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1824)
                     /locus_tag="ACHUOF_004389"
     CDS             complement(<1..1824)
                     /locus_tag="ACHUOF_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFR0613271.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2029)
                     /locus_tag="ACHUOF_004390"
     CDS             complement(1932..>2029)
                     /locus_tag="ACHUOF_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0613272.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          579 c          430 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagc
//
LOCUS       DAZOYF010000347         2014 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZOYF010000347 DAZOYF010000000
VERSION     DAZOYF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.356"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(73..1485)
                     /gene="pykF"
                     /locus_tag="ACHUOF_004391"
     CDS             complement(73..1485)
                     /gene="pykF"
                     /locus_tag="ACHUOF_004391"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR0613273.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1493..1687)
                     /locus_tag="ACHUOF_004392"
     CDS             complement(1493..1687)
                     /locus_tag="ACHUOF_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613274.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT          508 a          454 c          488 g          564 t
ORIGIN      
        1 ctggttatcg attaaataaa aaaagcgccc atcagggcgc ttcgatatac aaattaattc
       61 acaaaagcaa tattacagga cgtgaacaga tgcggtgtta gtagtgccgc tcggtaccag
      121 tgcaccagaa accataacta cgacgtcacc tttgtgtgcc agaccgctct gcagagccag
      181 ttctttaccc agacggtaga aatcatcagt agaagtgatc tctttaacca gctgcggcac
      241 aacgcctttg ctcagtacca actgatgagc cgttttttcg ttggtggtca gtgccaggat
      301 ggtggcatcc gggaagtatt tacgtactgc gcgagcagat ttaccgccct gggtagcaac
      361 cacgatcagc ggagcatcca gtttttcagc agtttcaacg gcaccacggc ataccgcttc
      421 ggtgatgcgc agtttacggt tgtcattgtt gaactcgaga cggctgttca tcacgcggtc
      481 ggtacgttcg cagatggtcg ccatgataga aaccgcttcc agcgggtatt taccttttgc
      541 ggattcacca gacagcatca ctgcgtcagt accgtcgagg atggcgtttg caacgtcacc
      601 ggcttctgcg cgagtcgggc gtgggttttt gatcatggaa tccagcatct gggtcgcagt
      661 gataacgact ttacgtgcac ggatacattt ttcgatcatc atcttctggg cgaagataac
      721 ttcttctacc gggatttcta cacccaggtc gccacgcgca accatgatgc cgtcagaggc
      781 ttcgaggatt tcgtcgaagt tgttgaggcc ttcctggttt tcgattttgg agatgatgtg
      841 gatgttttcg ccgccgtgcg ctttcaggtg ctcacggatt tcgataacgt cagaacgctt
      901 acgaataaag gaagcagcaa caaagtctac gcctttttcg caaccaaaga tcaggtcctg
      961 tttgtctttt tcagccagtg ctggcagagc aatggaaacg ccaggcaggt tcacaccttt
     1021 gttttcgccc aggtcaccgt tgttcagcac tttacagata actttgttac cttcaatggc
     1081 ggtaacttcc ataccgatca gaccatcgtc aaccagtacg gtgttgccaa cagacaggtc
     1141 agtagtgaaa ccttcatacg ttaccgcaac catttcgctg ttgccgataa cagatttatc
     1201 agtggtgaaa gtaaaggtct gaccagcttt cagagaaaca tcgttaccgc cttccagttt
     1261 catggtgcgg atttccggac ctttggtatc aagcaggata gcggcggttt taccagtttt
     1321 gctcatcacg ttgcgcagat tctgaatgcg ctgaccgtgt tctgcatagt caccatgaga
     1381 gaagttcaga cgcataacgt tcatgccagc gtccagcatt ttagctaaca tctcttcaga
     1441 ttcggttttc ggtccgatgg tgcaaacaat tttggtcttt ttcatgacag tcttagtctt
     1501 taagttgaga aggatgggag aaacttgctt tctgggcgca ctgccggaaa gcgaatccgg
     1561 tattacgaaa gtggtggtgc ctcgctctaa ggataggtga catcgaaaaa gcgtgcagag
     1621 gaatgtgtgc ttgtgtctca gcccaacgtg agtgtctgtt tttgagtcgt tgagttctac
     1681 agtccaaagt gctgaaacca ttcaagagtc aattggcgcg cattatacgc tgaaattaat
     1741 ccaaaaggaa aacaaaaaca gcgtttcaaa aaatatacaa cttgttagac aataaattgt
     1801 tatcaaagat ttaacgttcc agggaaaagg ttacgcaaaa acttcgctgt ttgcgcaacg
     1861 tctggtgccg aagttgtcag tgtgaggata agtgaaagtt catccggtgt ataaatatat
     1921 catcgctcaa ttgctacagt taaaataatt tatcgcgtta tttgtctgat tttgtttgat
     1981 ttttgtttaa ttggttagat tgatatatgc tgat
//
LOCUS       DAZOYF010000348         1994 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZOYF010000348 DAZOYF010000000
VERSION     DAZOYF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.357"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..494)
                     /locus_tag="ACHUOF_004393"
     CDS             complement(<1..494)
                     /locus_tag="ACHUOF_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR0613275.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(525..1217)
                     /gene="fimC"
                     /locus_tag="ACHUOF_004394"
     CDS             complement(525..1217)
                     /gene="fimC"
                     /locus_tag="ACHUOF_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR0613276.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1437..1979)
                     /gene="fimA"
                     /locus_tag="ACHUOF_004395"
     CDS             complement(1437..1979)
                     /gene="fimA"
                     /locus_tag="ACHUOF_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR0613277.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          582 a          419 c          426 g          567 t
ORIGIN      
        1 tgagcgctac tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
       61 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
      121 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
      181 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
      241 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg atacacgccc
      301 gcaggttgat gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct
      361 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca
      421 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
      481 gtgggtattt tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact
      541 tctctgaccg gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc
      601 actcctgagg gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac
      661 ttttggctgc ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca
      721 ttgatatagt taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct
      781 tgtaattgaa ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca
      841 ttcctgccgt tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac
      901 agagactcac gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg
      961 cttttgggtt cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg
     1021 ctgctgtttt caatccacga attgaccaga aagagctcat tggtatggct atttttaatc
     1081 cataccgcag tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc
     1141 cctccggcag catgggcgct ggccgaaatg attaaataaa atatcatgag catcaataac
     1201 tttattttag tcatcatagt taatacgcct tgtgtattcc tgacggcaaa aataaggagt
     1261 ataaagaaat gattgctttg gcttcttaca acacatcatt cccttttcat ttaataaagc
     1321 ggcatgatac aaagtgaacg tggtttcgat ttacattgcc accataccgc ttcagacaac
     1381 cggtacatta ctttgttata agacgtgata tataattcaa aacaacgtgg ttttgattat
     1441 tcgtatctca taataaaagt cgcgtcagca tttgcttgcc ctgctgatgc acttgtaccg
     1501 gtgcctttat aacgtgcaga aaaatgaaga acgttggtcc cggggatcaa gttctggttg
     1561 gttgagaagc tattcccatc aggtttgaga attgcggatg tattatcaag tatttcaata
     1621 ccgacacccg ttgctgttgt tgtatttgta ctggatacaa tggccaataa attatcattg
     1681 attgcatcag cctgaccaga aaatgcaacg gcagcattag cggcaacaac aggatcgcag
     1741 tcgttcaact gaatggtgaa tggaattaat gcagaggtat taccaacagc attgaaaata
     1801 tcggtacgat attgaccgag tgtgacaacc tggtctgccg agtcagtatt cactgaacag
     1861 gcagcattca ccagttcgcc ttcaaagtga attgtaccgc catctacaac ggcagcataa
     1921 ctacccgtag cggcgaatag tgcggcagcc agcgcagacg aaataaatct taatttcata
     1981 tatattcctt caat
//
LOCUS       DAZOYF010000349         1977 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZOYF010000349 DAZOYF010000000
VERSION     DAZOYF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.358"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..556)
                     /locus_tag="ACHUOF_004396"
     CDS             complement(<1..556)
                     /locus_tag="ACHUOF_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613278.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLF"
     gene            complement(727..1665)
                     /locus_tag="ACHUOF_004397"
                     /pseudo
     CDS             complement(727..1665)
                     /locus_tag="ACHUOF_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(1728..>1977)
                     /locus_tag="ACHUOF_004398"
     CDS             complement(1728..>1977)
                     /locus_tag="ACHUOF_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFR0613279.1"
                     /translation="RSNIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSY
                     LFGVLFDLLALSPIFTLFTFSLFSLVLGYCASKYFIFR"
BASE COUNT          756 a          384 c          286 g          551 t
ORIGIN      
        1 aaaacaaaga tagcgttaaa tcaatatatg cataaccaag atgatcttta agtggattga
       61 tagtaaatag taggattgta gcaataatgc ctggataata agttttcgta atttcccatg
      121 aacagccaac aagtccacag gctattacaa aaaaacagat atttgctaaa aactgagcta
      181 accttacatc tcctaactgt aaacctaaag ccattaaaag atctgcattt tgcgggaaca
      241 gaggaaaacg caaatattgt tcaacaacaa ttgcatggtg ctcaactaat gaacgagcca
      301 aaggtaagtg atacatagta tcatcccaat caccgggagc tcgcagggac gataacaatg
      361 taaccacaaa aatcaaaaca agaaataaaa taacaaacag cgtactgaac tcaatattat
      421 ttattgccag ccatctaaat tgctgacgtt taataatgct taaaacccaa cacaccgtaa
      481 aaattaaaaa gatagcaatc ttattaaatt gcccgaccat gcccagaaag taaattatca
      541 aaatatttaa agacatccct atgcataggc aataaaaaat aaagaaaatt tgttaagtcc
      601 tgccaccccc tccttaaaaa aagatgacaa tacaatatta ccaatgacaa aaaatgtcat
      661 tccaaaagca aaacaataca caacatacaa atacaacggc aattcaatca tagtaaaata
      721 ctctatttac attatcagtt tatagttttt ttcagaaaga taaaataatg ccttcatttc
      781 acgtctagtt gttgtatttg aatgcaaaat aatgccacat acaaatgcca ttattgacga
      841 taacatgatg gaggcagtta aaaaggcagt cggaaatctt ctcaccaaac ctgtatcaat
      901 ataatcaaca atgatgggca atcctaaaat tatagacaaa gcagcaagta aaacgccgat
      961 aatactaaaa aagtatagtg gccgttcaag cgagtaaagt ttaattattg ttttcagaat
     1021 cttgaaccca tcactccaag ttgatagttt gctcacactc ccttcagatc gctcgccata
     1081 ttttgtatta acctcaccat acttcatacg taattctaaa gcatgaattg taagctcagt
     1141 ttcgatttca aatccccttg atagacatgg aaaagattta acatagcgtc tggaaaatac
     1201 acgatacccc gacaacatat cagtaaaatt ccctttaaag atagtctgaa cagtaccagt
     1261 caacaattta tttcctcagc gatgcccttt tctgtatgta ttttgatctc cattctcttg
     1321 acgacatcct acaaccatat ccaattgatt ttttatgaga tggttaatca tttctggagc
     1381 agaacttgca tcataagtgt catctccatc aaccattaga taaatatccg catctacatc
     1441 tgagaacata cgtcttacga cattcccctt gcctttcaga ggaacagagt gaactgttgc
     1501 tccagcatcc tctgccactt gtgctgtact gtcagtcgaa ttattatcaa atacatatat
     1561 agaagcagtt ggaatattcg ttttgaaatc atttatgact tttgctattg ccttctgctc
     1621 attataacaa ggaataatta cagcgatatt aaaagagtga tacataagcc cctcatataa
     1681 actatttagc taatgtgcaa gtaattgtgt ttttaacaaa tcgtttacta acgaaaaatg
     1741 aaatattttg atgcgcaata tcctaaaacc aaactaaaaa gagaaaaggt gaacaatgta
     1801 aatattggag ataaagctaa gagatcaaat aaaacgccga ataaataact catcgccccc
     1861 ataaaaaata taaatataaa atatctagcc ttggttgcag atactttaaa tgagcatctt
     1921 gcatttgcaa aaaaagaaaa ggttgctgca catataaaac caacaatgtt acttcgg
//
LOCUS       DAZOYF010000350         1969 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZOYF010000350 DAZOYF010000000
VERSION     DAZOYF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.359"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            240..509
                     /gene="yacA"
                     /locus_tag="ACHUOF_004399"
                     /pseudo
     CDS             240..509
                     /gene="yacA"
                     /locus_tag="ACHUOF_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
     gene            509..678
                     /gene="yacB"
                     /locus_tag="ACHUOF_004400"
                     /pseudo
     CDS             509..678
                     /gene="yacB"
                     /locus_tag="ACHUOF_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="ACHUOF_004401"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="ACHUOF_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFR0613280.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1708..>1969
                     /locus_tag="ACHUOF_004402"
     CDS             1708..>1969
                     /locus_tag="ACHUOF_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0613281.1"
                     /translation="MTEALRLHFEEHLPRVVAGCRLGVPKSTACGMFVRFRNAGLSWP
                     LPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRR"
BASE COUNT          560 a          433 c          462 g          514 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgcctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa
     1921 agcaccgtta tgcccaaact cccggtggta aaaaaacgtc cccggcggc
//
LOCUS       DAZOYF010000351         1922 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZOYF010000351 DAZOYF010000000
VERSION     DAZOYF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.360"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            53..934
                     /gene="spa33"
                     /locus_tag="ACHUOF_004403"
                     /gene_synonym="spaO"
     CDS             53..934
                     /gene="spa33"
                     /locus_tag="ACHUOF_004403"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFR0613282.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            939..1589
                     /gene="spa24"
                     /locus_tag="ACHUOF_004404"
                     /gene_synonym="spaP"
     CDS             939..1589
                     /gene="spa24"
                     /locus_tag="ACHUOF_004404"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFR0613283.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            1604..1864
                     /gene="spaQ"
                     /locus_tag="ACHUOF_004405"
     CDS             1604..1864
                     /gene="spaQ"
                     /locus_tag="ACHUOF_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFR0613284.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
BASE COUNT          574 a          268 c          372 g          708 t
ORIGIN      
        1 ataaggggaa taaaaataat agtacaggat ataatgaaca gagtgaagaa gaatgctaag
       61 aattaaacat tttgacgcta acgaaaaact acagatttta tatgcaaagc aactctgcga
      121 gcgtttttcg attcagacat tcaaaaataa atttacaggc agtgaaagtt tagtcactct
      181 tacttccgtg tgtggggatt gggtaattcg tattgataca ttatcttttt tgaaaaaaaa
      241 atacgaggta ttttcaggat tttctacaca agaatcttta ctgcatttat caaaatgtgt
      301 ctttatagag tcgtcatctg tattttcgat tccagaactg tctgataaga ttactttccg
      361 gatcacgaat gaaatccagt atgcaactac tgggagtcat ttatgctgtt tttcctcttc
      421 tttaggtatt atttattttg acaagatgcc ggtattacgt aatcaagttt ctcttgactc
      481 attgcatcat cttttagagt tttgcttagg ttcatctaat gtaaggctgg ctactttaaa
      541 aagaattcgc actggtgata taatcatagt tcagaaactt tataatttat tattgtgtaa
      601 tcaagttatt attggggatt atattgtgaa tgataataat gaggcaaaaa ttaatctgtc
      661 agaaagtaat ggtgagtcag aacacacaga agtttctttg gcattattca attatgatga
      721 tatcaatgta aaagtggact ttattctttt agaaaaaaat atgacaatca atgaactaaa
      781 aatgtatgta gaaaacgaat tattcaagtt tcccgatgac atagttaaac atgtaaatat
      841 taaagtaaat ggttctttgg ttgggcatgg ggaacttgtt tctattgagg atggttatgg
      901 tatcgagatt agttcttgga tggtaaagga gtaatctcat gctgagtgac atgtccctca
      961 tcgctacact ttcttttttt acgctattac catttttagt tgctgctggt acctgttata
     1021 taaagttttc tattgttttt gtaatggtac gaaatgcact tggtttgcag caggttccat
     1081 caaatatgac tctcaatggt attgcattga ttatggcttt gttcgtgatg aaacccatta
     1141 ttgaagctgg ttatgaaaat tatctaaatg gccctcaaaa atttgataca atatctgata
     1201 ttgtccgttt ttctgattct ggcttgatgg aatataagca atacttgaaa aagcatacag
     1261 atcttgagtt agcacgtttt ttccaaaggt ctgaagaaga aaatgctgat ttaaagagtg
     1321 cagagaataa tgattattca ttgttttccc tcttgcctgc atatgcgcta agtgaaatca
     1381 aagatgcgtt caaaataggt ttttatttat atttgccatt tgttgttgtt gatcttgtta
     1441 tttcaagcat tttacttgca cttggtatga tgatgatgag tccaatcaca atatcagtac
     1501 caataaaact tgtattattt gttgcgcttg atgggtgggg tatcctgtct aaggccttga
     1561 ttgaacaata tatcaatatt cctgcttaga tgatggaggt aatgtgtctg atatagttta
     1621 tatgggtaat aaggctcttt atttaatcct tatcttttcc ttatggccag taggtatagc
     1681 tacggttatt ggattatcta ttggtttgtt acagacagtg actcaactcc aagagcagac
     1741 acttcctttt ggtataaagc ttataggtgt ctcaatatct ttgctacttc tttctggatg
     1801 gtatggtgag gttttattgt ctttttgtca tgaaataatg tttttaatta agagtggggt
     1861 ttgatggaca tttcaagctg gttcgaaagt attcatgtgt ttttaatact cctgaacggc
     1921 gt
//
LOCUS       DAZOYF010000352         1920 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZOYF010000352 DAZOYF010000000
VERSION     DAZOYF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.361"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHUOF_004406"
     CDS             <1..203
                     /locus_tag="ACHUOF_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613285.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="ACHUOF_004407"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="ACHUOF_004407"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR0613286.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          473 a          475 c          448 g          524 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
//
LOCUS       DAZOYF010000353         1918 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZOYF010000353 DAZOYF010000000
VERSION     DAZOYF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.362"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(53..1471)
                     /locus_tag="ACHUOF_004408"
     CDS             complement(53..1471)
                     /locus_tag="ACHUOF_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR0613287.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGCEQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            complement(1472..>1918)
                     /locus_tag="ACHUOF_004409"
     CDS             complement(1472..>1918)
                     /locus_tag="ACHUOF_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR0613288.1"
                     /translation="VASNATLTDVEPQSMEDGVNDLEMMMAEWLGGDVSPGINVGYIF
                     ADADVAPEPGDEHGLSNNAINAVIFNLACRIAPDYALEAPAKLITTARYGKERLVKLS
                     AMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEPCDNGGE"
BASE COUNT          465 a          512 c          462 g          479 t
ORIGIN      
        1 tcgagccgtg tagcctgaat gattaccggc acattgagat tcgaatccgc cattactcaa
       61 tccttatctg gcagccagac agagtgacag gtgacttcgt gataacgcgc aatttgaagc
      121 cgacattttt cctgatgcgc cctactcgct tccacaaaac gcgtttgtcg taaacgaacg
      181 gttcattctg ctcaatcatc tgctcacacc cgtaattgat gccgtcagtg gttgcagaga
      241 gaaaaaggcg gtcagcatac tgcgcaacgc cagttgacga ttcaacctca aggtcgaaca
      301 ctctggcgtt atccgctttg aacagtggag taaacagcag gtgttcctgt tgcttgtcgt
      361 actggctgct gatgtcgaat tgcaatttcc cggtcacgga ctccagctta tcgccgcacg
      421 ttatttgatt gccttcgtaa atgaagtcga tagcgcggta cacatcgtca tacaggcctg
      481 ttttcagcac acaccattgc ggaccattgg cgcttgaaga tgcgtcgtac accagtacat
      541 ggcgcggaag gtggataatc agcaactcat gcgcatcaaa ccgcagcgat tccatcacgc
      601 catcagccag ttcatcagca gtgtaggagc ggaggatttt ctcaatgctc gcgctggcga
      661 ttggtgacac ctgaccggag ccgatgatgt atacagacgg cgcacctgtt gccggattgc
      721 tgatgaacgc ataagaatca gcgaatggtg ttttgcagta agtcccggca atacctttct
      781 gcaccatcag cgatggctgt gcgacataca aagcggcacc aacggtggtt gcacctgtca
      841 gggaaaaata ttcaatcgtc gatgagccaa agcagacgat gaagtctcgc catgtaccta
      901 tgccgatgat gccgtccggc tgagactcgg cacgatattg tgcactgtag cggtcaggat
      961 gcgattcatc ttcaaggtca gtgataaacc atgaatcagt gccgtctttt gaccacgcat
     1021 aacgcccacg taagcgcgta atgtcgcgaa ctgaacctaa ctcatactga gtgaatccac
     1081 tgtctgtagg ccagtttgag acggttttaa ccgtgccatc atagcgatac tcgaccagtt
     1141 gaccattaac gcctaccgcc tgtgatgtcc gaccatgcgc cattgatacg cgaccacttc
     1201 cggcaacatc accgacctcg ctttctccct tatacaactt gccaccgcaa acgcgataaa
     1261 cagcattctg cgccatgttg tactcgacac cgcgcgatac accattcaca tcagaacgtt
     1321 tggcaatgcc cgggaatgag cgaagatatc cgctgctgtt gagtatttct ttgggtgtag
     1381 ccagcatatt cactggcaga tagtcgatgt agtcggcgtt tcggaaatct ttgccgacac
     1441 ctttcataag cggaagttgc tgaatcggca tttattcgcc cccgttatcg caaggttcct
     1501 tccggtggaa gtaattccaa ccattccact tcgccaactg gttaccgcta ccaacaggca
     1561 tacggtttgg ataaccggac ttacatttag cggcttttgc tctgtccatt gcagacagtt
     1621 tgacgagtcg ctctttcccg tatctggcag tggttataag ttttgctggc gcttccagcg
     1681 cataatctgg agcaatgcgg caggcaaggt tgaaaatgac ggcattgata gcgttatttg
     1741 ataaaccgtg ctcatcgccc ggttctggag cgacatctgc atcagcgaaa atgtagccaa
     1801 cgttgatacc aggtgacaca tcaccgccaa gccattcagc catcatcatt tcaaggtcgt
     1861 tgacgccgtc ttccatagac tgcggttcta catcggttaa cgtggcattt gatgccac
//
LOCUS       DAZOYF010000354         1913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZOYF010000354 DAZOYF010000000
VERSION     DAZOYF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.363"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(53..1702)
                     /locus_tag="ACHUOF_004410"
     CDS             complement(53..1702)
                     /locus_tag="ACHUOF_004410"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR0613289.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAZOYF010000355         1884 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZOYF010000355 DAZOYF010000000
VERSION     DAZOYF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.364"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..368
                     /locus_tag="ACHUOF_004411"
     CDS             <1..368
                     /locus_tag="ACHUOF_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613290.1"
                     /translation="LSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIMA
                     GFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRFL
                     IFNTEQSGFYEINDVYVRK"
     gene            602..730
                     /locus_tag="ACHUOF_004412"
                     /pseudo
     CDS             602..730
                     /locus_tag="ACHUOF_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            920..1324
                     /locus_tag="ACHUOF_004413"
     CDS             920..1324
                     /locus_tag="ACHUOF_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613291.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            1387..1884
                     /locus_tag="ACHUOF_004414"
                     /pseudo
     CDS             1387..1884
                     /locus_tag="ACHUOF_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          529 a          414 c          458 g          483 t
ORIGIN      
        1 cattatcact ttcattcatt attagttcat tggttatttc aataataatt cgtatatatg
       61 gacggatctt ttctgtatca tgtttttcag atatcccttt tgttagagaa caccgcatta
      121 agttaattat ggctggtttt ttggtttatt tttggttttt ttcctttctt gttggctggt
      181 ataaacctca attaaaaaaa gaatatcaga tgctttgtta taataatagt tggtattatg
      241 ttcttgctcg ttatgattca cggctcgttt tatctagttc atttaaagat gacagtaaca
      301 gattcttaat atttaataca gaacagtcgg gattttatga aatcaatgat gtttatgtca
      361 gaaagtaata atcctctccc acaaagtgaa ttaaatgaat atataatgcg ttcgaagtat
      421 atgtattgag ctgtcatact gagaaacaaa ccttagggca gcacaattaa agccagaaac
      481 aactatgcaa gaaagttact aaataagtgc tcggaacagt aaaacactca ggggggactc
      541 agtccggatt atgtaatcag ataaattgcc aaacatcaca aatcaccagc cggactgagt
      601 gatgccgagc attgcaccaa taccccgtga tgaacgatgc ctgatgcaga aagctcccta
      661 taaaacaaag aacaaaaact acgaccggcg attcaccgtc atactgatgc tgcatcgtgg
      721 tgacactgtc tgtcactttc atgttgactg aacaactctg ctgcgaagaa ggtgtaccgc
      781 cggcgggatt tgattgtccg gcgtctagct tccacttccc tgatggaact gaaagttgtt
      841 atggccggtc agcttgccct gtccgcagga catgaggtgc tgtgacggac agcaccagta
      901 acggaaagtg acaatgaaaa tgaatactgc gctgatagtt gcactaatgt gtatgtggta
      961 tgcggtaccc gctgctgcaa aagagacttt actggctatg ccacgtaatt caacagaaca
     1021 ttgctatgcg gagattaatg tgcacggacc atatggggtg tatttccgag tggtcccgca
     1081 tccccccggg ggaaagagct gggtcgaatg taacagtgac tactattact ctgataaacc
     1141 accgggagta cagatactcg gaacccgtgc agggtgtcgg gtatacggaa tctgcgggac
     1201 cacctcgaca ctgcatgtgg ctggaagagg ggttgtgtgt attaaaaata tatgcagtcc
     1261 acgtgggatg ataattcaca ggatacggaa acgtcctgta gtggctgtaa gcgatgagat
     1321 gtgatcttac ccagcaatag tagacacgcg gctaagtgag taaactctca gtcagaggtg
     1381 actcacatga caaaaacagt atcaaccagc aaaaaaacac gtaaacagca ttcgcctgaa
     1441 ttttgcagtg aagccctgaa gcttgctgaa cgcatcggtg ttgctgccgc agcccgtgaa
     1501 ctcagcctgt atgaatctca gctctatgcg tggcgcagca aactgcagca acagatgacc
     1561 tcgtctgagc gtgaaagtga actgcccgcc tgaagcggca actggcggag caggcagagg
     1621 aactggctat cctccaaaag gccgcgacat actttgcgca gcggctggta tacgtggtgt
     1681 cagcgtcgga caggaataag ccctcgtcag cagttccgtc agcactgcga cagcgttgtc
     1741 ctcgcggcgg cctttacccg gtcaaaacag cgttacggtg caccacgcct gacggatgaa
     1801 ctgcgtgctc agggttacca ctttaacgta aaaaccgtgg cggcaagcct gcgccgtcag
     1861 gggctgaggg caaaggcctc ccgg
//
LOCUS       DAZOYF010000356         1881 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZOYF010000356 DAZOYF010000000
VERSION     DAZOYF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.365"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1135)
                     /gene="pdeF"
                     /locus_tag="ACHUOF_004415"
     CDS             complement(<1..1135)
                     /gene="pdeF"
                     /locus_tag="ACHUOF_004415"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR0613292.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
     gene            complement(1317..1469)
                     /locus_tag="ACHUOF_004416"
     CDS             complement(1317..1469)
                     /locus_tag="ACHUOF_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613293.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            1487..1678
                     /locus_tag="ACHUOF_004417"
     CDS             1487..1678
                     /locus_tag="ACHUOF_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR0613294.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          594 a          415 c          378 g          494 t
ORIGIN      
        1 attgaatccg cagcatgatg ccatagttct taaccagcat ttccatgcca gggatgcgta
       61 aataacaaag cgcagaccag ggggcatcac gcaacgcgcg attcagggcg cgaacatttg
      121 gcagatgaac caccgggtcc acatacgcaa gccgctgaat gcgtctgact accgctcgct
      181 gacgagttgc cagtacagcc atataattga caataaaaga gaataccaga taactggagg
      241 aggttatggt cagctgcgtg gtatagccag gataaatggg aatgtaattt tgatagctgt
      301 ggatgctgat catcaacacg accgcccaga gcagcgaaat cagcttataa ccatagcgca
      361 ttgctcccca catcatcagg ggcagcaata atgacaaggt ataattggtg ctaaaaattg
      421 tgcttttttc atttaacggc atgcataaca gcaacaataa agcacctaat gccagtagcc
      481 agagcgcgaa ctcttttttg gtgactttgg catcaacctg ctgttttaat tgcgaataat
      541 agctacgtaa ataaaatgga tttcgcacta cccgaatgat gaagtagcac agcgggacac
      601 cgatcagatt acccaccagt aaggcctgat aattgattaa agtcccgagg ttaaaaggca
      661 tgacaccgac cagattttct ctgctcgcca gtaatcctac aaatgcagca aactggaaaa
      721 gtatcagaaa cagcgttgca ggaaacacta tctgccagaa tatacgctgg gaaattaaac
      781 gggtatcacc atgtgaaacg ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac
      841 agagaatgat aatgataata aaattagccg ttaatgagag cgtttcgtaa aaacccagct
      901 ctgcatattt gcggacaaaa atccctaagg tgatccctgg caatgccgcc cagctgaaaa
      961 acatcatcat gctgatcatc agtgccaaag gcagataaaa gagaaaaacc tcaccggaag
     1021 aaatatgcgc gaaagtatta atgtgggcga aaatgggcaa gattaaagaa ggcaggaaca
     1081 gcggaagccc ccaccattta tcacgtattt ttatataagt tgcattcagt ttcatagatg
     1141 ctcagcagaa tcccccacat cctgaaggag gtgtattcag acaggcatcc cacctgactt
     1201 cgaatgatga ttattcatca ctatagagag cattgattct aagtgtcata tgaaagttcc
     1261 aattgatata tatcaaacaa aataaccctg attaatgaat tattacgttt atcatgttaa
     1321 ttcatcatta ttacatcatc attgtaaata attaaattaa cttccataac attaaaatat
     1381 gtatccactg acgctttttt acataacgaa gaattgacca ttttgtcctg ttgtgcctta
     1441 atgtaagtac cgtccacagc gtgggacata cttcaaggaa ccttttgtga gtcaggcaac
     1501 tagtatgcga aaacgacacc gatttaacag tcgcatgacc cgtatcgtac tgctcatcag
     1561 ctttatcttc ttctttggcc gttttatcta ctcgtccgtc ggtgcctggc agcaccatca
     1621 gagcaaaaaa gaagctcagc aatccacact ctccgtcgaa tcaccggtac aacgttagcg
     1681 gttaccttct ccactttcac agaacataac ggcacttcgc tgtcggatgc ttttgctctt
     1741 tgggattatc aaagcggcag atattctttc atcttaaatt ttacgtcttt gtcctgactg
     1801 atgtttatcc tgtttggctg cgaaataaat ataaaattaa tatatatgtt gtaatgatat
     1861 atttttataa attattccct g
//
LOCUS       DAZOYF010000357         1847 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZOYF010000357 DAZOYF010000000
VERSION     DAZOYF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.366"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(1..355)
                     /locus_tag="ACHUOF_004418"
                     /pseudo
     CDS             complement(1..355)
                     /locus_tag="ACHUOF_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(355..1275)
                     /locus_tag="ACHUOF_004419"
     CDS             complement(355..1275)
                     /locus_tag="ACHUOF_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0613295.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1413..>1847
                     /gene="pgrR"
                     /locus_tag="ACHUOF_004420"
     CDS             1413..>1847
                     /gene="pgrR"
                     /locus_tag="ACHUOF_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR0613296.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          461 a          454 c          426 g          506 t
ORIGIN      
        1 ggcacccccc acacacaccc agcacgccaa tgcgttcagc atcaacatat ggcagagtta
       61 ctgcataatc gataacacgg ctaatatcct ctacgcgctg tgtcggatct tctatccaac
      121 gaggctaccc accagattct ccctggaaac tggcgtcata agcgagaacg acatagcctt
      181 tttcagccaa tgccttgcca tagatattgc cagacgtctg ctccttacaa ctgccaaaag
      241 gatggacgct gataatcgcc ggatactgtt ttgactcatc aaaatttggt ggaaactgaa
      301 tattggcagc aatatgccag gccatatcac tattgcggat ttgaatatgt ttcattacag
      361 agtttcctca aagaacggag ctaatactga aattgcttca gccacatagg ctttgccgtc
      421 ataaagatcc atatggtttg cgccttcaac aatgtgatag cgtttatcct ggcttgatgc
      481 ccgatcgtac aggtcgtcac tcatccattt gctccctgcc tggctgcccg ccacaatctg
      541 catcggctga gtcaggtaca cttccgccat atggtaagca tcataggtaa taatctggtt
      601 aaggctgcgc aaagtagcgt aacccggtgc tgttggatac tgcgcacgag gtgtgtggta
      661 atactcccag gcctgacgca gttcttcgtt cggcgcatcg gcctccttca ttggagccag
      721 tggcataatg gcgtattctt cgctgctaat atcgctggtt cgggcgtttg agccagcttc
      781 aatgtgtggc agagcatcaa tagatttcac attgttttcc caaccattgc ggaacatcga
      841 accaatattg accgcactaa cggtaccgat ggccttgatg cggcgatcct gaattgcagc
      901 attggctgta tatcctgcac cggcacaaat tcccatcgca ccaattcggg tattgtcgac
      961 ataagaaagc gttgtcaggt aatcaatcac ggcactgacg tcttcagtac gaatgtatgg
     1021 gttttctaac tgacgcggct cgccgccact ttcaccttga taagatgcgt cataagcaat
     1081 agtgacaaat cccttttccg ccagcttttc ggcataggtt ccggccgttt gctctttaac
     1141 gcccccacct gggtgagata ccacaattgc cggatactga cgggtttcat caaattttgg
     1201 agggaaatag atcactgcag acaaagagat ggttggatta ttgctgttag tgaagctgac
     1261 tttattattc atcattcagt tcctcatgga gttgtcggtt cgttttgacg gttggtgata
     1321 tcactataga tattgatcat taagttgatt agaccctaaa tcatgattag acctatcatt
     1381 taaatgatta atagattgta tggagatact gaatgaagcg tgaagaaatc gctgatctga
     1441 tggcgtttgt cgtcgttgca gaggagcgta gcttcactcg cgcagcagcc cgcctgagca
     1501 tggtgcagtc agctttaagc cagatagtgc gccgtataga agaacgattg ggattgcggc
     1561 ttctgacgcg aaccacgcgc agcgttgtac ccactgaagc gggcgagcat cttttgtctg
     1621 ttcttggccc tatattgcat gacatagatt ctgccctgac atccctgagc gatctgcaga
     1681 accgcccatc cgggacaata cgtattacta ctgtagaaca tgcagcaaaa acgatattgt
     1741 taccagccat gcgcacattc ctgaaatcgc atcctgaaat tgatattcag cttaccattg
     1801 attatggttt gaccgatgtc gtttctgaac gttttgatgc aggcgtc
//
LOCUS       DAZOYF010000358         1846 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZOYF010000358 DAZOYF010000000
VERSION     DAZOYF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.367"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..254)
                     /gene="ydfV"
                     /locus_tag="ACHUOF_004421"
     CDS             complement(<1..254)
                     /gene="ydfV"
                     /locus_tag="ACHUOF_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFR0613297.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFS"
     gene            279..518
                     /gene="relB"
                     /locus_tag="ACHUOF_004422"
     CDS             279..518
                     /gene="relB"
                     /locus_tag="ACHUOF_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFR0613298.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            518..805
                     /gene="relE"
                     /locus_tag="ACHUOF_004423"
     CDS             518..805
                     /gene="relE"
                     /locus_tag="ACHUOF_004423"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFR0613299.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            877..1032
                     /gene="hokD"
                     /locus_tag="ACHUOF_004424"
     CDS             877..1032
                     /gene="hokD"
                     /locus_tag="ACHUOF_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0613300.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1250..1501
                     /gene="rem"
                     /locus_tag="ACHUOF_004425"
     CDS             1250..1501
                     /gene="rem"
                     /locus_tag="ACHUOF_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFR0613301.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1568..1846
                     /locus_tag="ACHUOF_004426"
                     /pseudo
     CDS             1568..1846
                     /locus_tag="ACHUOF_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          506 a          409 c          466 g          465 t
ORIGIN      
        1 atgctaaagc aaaaaataga tgaataagtt gagttgtgca tatgtagcct gaccgtcaca
       61 aagtatatgg tgtctgtacc agtaagatga tggccggact ctttaaaaaa gagctgacct
      121 gcacaataca ggatggactt agcaatggct gctcctggca caaagcggac agtgatcacc
      181 gttcttacga ctactttctg acttccttcg tgacttgccc taagcatgtt gtagtgcgat
      241 acttgtaatg acatttgtaa ttacaagagg tgtaagacat gggtagcatt aacctgcgta
      301 ttgacgatga acttaaagcg cgttcttacg ccgcgcttga aaaaatgggt gtaactcctt
      361 ctgaagcgct tcgtctcatg ctcgagtata tcgctgacaa tgaacgcttg ccgttcaaac
      421 agacactcct gagtgatgaa gatgctgaac ttgtggagat agtgaaagaa cggcttcgta
      481 atcctaagcc agtacgtgtg acgctggatg aactctgatg gcgtattttc tggattttga
      541 cgagcgggca ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac agttgaaaaa
      601 gaagctggtt gaagtacttg agtcaccccg gattgaagca aacaagctcc gtggtatgcc
      661 tgattgttac aagattaagc tccggtcttc aggctatcgc cttgtatacc aggttataga
      721 cgagaaagtt gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg aagtatatag
      781 cgaggcggtc aaacgcattc tctgaaccaa agcatgacat ctctgtttcg caccgaaggt
      841 gacacttctg ctttgcgttg acaggagaag caggctatga agcagcaaaa ggcgatgtta
      901 atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac gaggaaagac
      961 ctctgcgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcac agcttacgaa
     1021 cctgaggagt aagagacccg gcgagtgaga aatccctcgc cacctctgat gtggcaggca
     1081 tcctcaacgc acccgcactt aacccgcttc ggcggttttt tgtttttatt ttcaacgcat
     1141 ttgaagttct ggatggtgcc ggaatagaat caaaaatact taagtagcgc gcagggataa
     1201 aagggatgga ccccgaacag gggagtgcta cttatctgga aggattctga tgatgaacat
     1261 cgaagaactg cgtaaaattt tttgtgaaga tggcctctat gctgtgtgcg ttgaaaatgg
     1321 aaatattgtt agtcattacc gcattgtgtg tttgcaaaaa aatggggctg cgttaattaa
     1381 ttttgtggat gcccgagtga cggacggatt tatcttgcgc gacggtgagt ttgtcacttc
     1441 attacaggca ttgaaagaga tcggaataaa agctggcttt tctgcttttt cagaagaata
     1501 aactcatcta caatcttgcg cggggctgaa ctcccgctga gtaacaccgt gccaccggag
     1561 aaaaccgatg gcacgcaacg caaaatatta caatcatgat aattcgaccg ttcttgccca
     1621 cacgcacgag cggtattctc acgcatttaa gtcagactgg taccagcatc cctcatgcac
     1681 tgaagaacag gccgaatggc ttattcagtg ttaccgcagg cgcggatgcg aggttaaaaa
     1741 agcccttagc ctcgactacc gtcactagat aatctccgtc aggctccctt actccgaaca
     1801 gccaccgcgt ccgtcccgca cattccagca acggatctgg aggtaa
//
LOCUS       DAZOYF010000359         1834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZOYF010000359 DAZOYF010000000
VERSION     DAZOYF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.368"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..182)
                     /locus_tag="ACHUOF_004427"
     CDS             complement(<1..182)
                     /locus_tag="ACHUOF_004427"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613302.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(323..877)
                     /locus_tag="ACHUOF_004428"
     CDS             complement(323..877)
                     /locus_tag="ACHUOF_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR0613303.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(874..1164)
                     /locus_tag="ACHUOF_004429"
     CDS             complement(874..1164)
                     /locus_tag="ACHUOF_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR0613304.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1164..1763)
                     /locus_tag="ACHUOF_004430"
     CDS             complement(1164..1763)
                     /locus_tag="ACHUOF_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0613305.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          496 c          422 g          471 t
ORIGIN      
        1 tggttatggg tgatcgggga gagagtttca acagagattt gagtggtcat aacgataact
       61 ccgtacattt ggaaattatc gccaccgacg acgccaatcg aactggtggc gaactgtgca
      121 gggttggcgt aaccgggtac ggaaaccggc gagcctttcg gctcccccac acagcccgcc
      181 ataaatcgcg aatgtgactg tgcaaacgat atgaaaaaag acgcgggcgc gtctcatatc
      241 gctccgtaaa catccgggac gccaatcccg acgccagatt ttgctggcgc gtgaggaata
      301 tagccccgga taacagattg agtcaacaaa cggtttttag atccccggaa gagaatgcat
      361 cacgcatcgg tagatagagc atatattcag caagatttag ccatacatct acccgactgc
      421 agcacgtccg gaaacaccac tcagggtgtg cgtcattcag caattcagcc attttacgct
      481 tagtcatccc ccgcccttca taccgttgcc ggaggacact aatcaatcca ggatgctctg
      541 ccagcacctc acttatgact ctatcaatac ataacgcctc tgcatcagta caatgcgcca
      601 gccaggtctt ttgcttgcca ttgatcatct ctcgcaaaaa cgcttccagc tcaggtttct
      661 ctattcccgc ttttttcatc ctgcgcaggg cttcattgac ggctgttttc gtcaattttt
      721 tggatgccag caactggtta aacatatttc ccgtcttacc gccgccaata tacgaccagc
      781 gcccccacat gcgcagtttt ccctgaatcc agacactttc cagcgtggtg agacgaaggt
      841 gttccccgct tttgcctgta tttgttgggt aaatcataaa taaccttcct ttctccagat
      901 ttcttgtgtg cgaaaaacac cttctgcatg catcaggcgt aattcttctt tggtgtaatc
      961 gctggttttt acccgcccgt cgattaaatc gtggcatgag ctacaggcaa tcgctgcctg
     1021 catatcgtgt ggttttgtcg ctgttccgca cgtccccgcc agcctgtaat gcgccagcac
     1081 agaagtttcg ggattgtgat tgcagtagcc aggaattctg acggtgcaca tctgcccccg
     1141 cgccgcttta cgtaaatcca ccattacgca aactccagta actgcgcggc cacattttcg
     1201 acttcctccg gagaggaaaa tttacggaac aggatccagt tccacagcac attcagtaca
     1261 gatttataaa cctgctgaaa ctcggtttcg tccatgttcg caaatgcgat agattttgcc
     1321 ctgcgcccac gactaccatc aggataaata tgctcggtgt aaaatccggc ctgaatggtt
     1381 acccactcgc ggaaagcgtc aaacgacttt agcaatgccg tatcccgggt tctgcatgtc
     1441 gcaactgtat taaggtattg ctctgcggca tcactcaggg ctggagtgtg ttcccgacct
     1501 actgattcgc acaggtaatc aacgaagcct gataccagtt ttcgttcgcg aggcgtgatc
     1561 gccccaccga ccggagtcca gtaatcaaat cccagttgca ggagtttgaa aaaacgcttg
     1621 tggaatgcgt agttacgcac acgcttaaag tctgcgtgta tccactcacc tattttgatt
     1681 tgatgcaaaa aatcgcaact ctccggcgtc gccgggagaa gtaatccgga agaggtttgt
     1741 ttgaccagtt gtatatgcgc catcgtagtt ctccgctggc gcagtagaat gggtgttcag
     1801 cccgttatgt agtataccag aattaatgcc aata
//
LOCUS       DAZOYF010000360         1812 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZOYF010000360 DAZOYF010000000
VERSION     DAZOYF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.369"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            100..293
                     /locus_tag="ACHUOF_004431"
                     /pseudo
     CDS             100..293
                     /locus_tag="ACHUOF_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            436..657
                     /locus_tag="ACHUOF_004432"
                     /pseudo
     CDS             436..657
                     /locus_tag="ACHUOF_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(678..740)
                     /locus_tag="ACHUOF_004433"
                     /pseudo
     CDS             complement(678..740)
                     /locus_tag="ACHUOF_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1190..>1812
                     /gene="ospC1"
                     /locus_tag="ACHUOF_004434"
     CDS             1190..>1812
                     /gene="ospC1"
                     /locus_tag="ACHUOF_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFR0613306.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGA"
BASE COUNT          626 a          362 c          329 g          495 t
ORIGIN      
        1 tttagctgaa cgggccatat aaccacctga aagacaatga cattgcctgt ttttataacg
       61 gtaattgcag accatgacaa accgcagccg tcaggctgcc tactcgagtc acaaggctct
      121 ttgtgtccga tatgccggca gcgcattacc catgaaactg gctggaacgt acaccataag
      181 gtgaagaaag ttatgggggc ggtgaagaat tatctaacct tgtcttgtta catccaaatt
      241 gccatcgaca gttacacagt tgtgaagccg gctctctcaa aaagggctta taaaggcttg
      301 agcggtgtac cgggaaaccg gtatgcaccg ttcttagggg agggcagtcc agcaatgaac
      361 tgtccttacc cgacaaacat ccaaaacgaa cggaacgtgc tggaatccgc atataacccg
      421 ttgtaaagtg aggaaatgga cacctcactt gctcatgaga acgcccgcct gcgggcactg
      481 ttgcagacgc aacaggacac tatccaccag atggctgaat acaaccgcct gctctcacag
      541 cggatggcgg cttatgcttc cgaaatcaac cgactgaagg cgctggttgc gaaactgcag
      601 cgtatgcagt tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca
      661 ttttcacggg cgatctacgc cacacaggct ctgggttgca gtgactgctc cattaaggcg
      721 attttgaact cataagggaa gtaacaaaaa aactgcaaaa gtacaataat tatatatggc
      781 caaactaacc tctattaaaa aacatcagtt tttcacactt agttgagctt catgaaaaaa
      841 tcatcgagga tttcattgca gaatacacat tccactaact ggaagcactc cataagagga
      901 atcagagaaa gtatcgtttt tttatagtaa aattcattgc tgttcaataa aattgatata
      961 gttattaaat tatgaaaata atttcacaaa aaagattaac ttgagttctt aagcatcgga
     1021 ttttagatat tttgaaatat ctggcatcac ataacatcaa aagtatataa tactcttatc
     1081 taattacatg cagtatattg tatatatact caaaaattgt aattcatacc ctgctatatc
     1141 ttgtagggaa agatattaat taaaactgtt ttcatataag gttcatttta tgaatatatc
     1201 agaaacactg aactcagcaa atacccaatg caatatagat tctatggata acagattaca
     1261 tacattgttt ccaaaagtga catcagtgcg aaacgctgca caacaaacta tgccagatga
     1321 aaaaaattta aaagatagtg caaatattat taaaagtttc tttaggaaaa ctatagcagc
     1381 acagagttat agtagaatgt tctctcaagg ctctaacttt aaatctttaa atatagcaat
     1441 tgatgcacca tcagacgcta aagcctcatt taaggctatt gagcaccttg acagattatc
     1501 gaagcattat atatctgaaa taagggaaaa acttcatcct ctttctgcag aggaactcaa
     1561 tttgctttcg ctaattatta attctgattt aatcttcaga catcaaagta attctgattt
     1621 gtctgataaa attttaaaca ttaagtcatt caataaaatt cagtctgaag gaatatgcac
     1681 aaaacgaaac acatacgctg atgatataaa aaaaatagct aatcatgact ttgtgttttt
     1741 tggcgttgaa atctctaacc atcagaaaaa acaccccctg aatacaaaac atcacactgt
     1801 tgattttggt gc
//
LOCUS       DAZOYF010000361         1809 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZOYF010000361 DAZOYF010000000
VERSION     DAZOYF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.371"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            173..1198
                     /locus_tag="ACHUOF_004435"
     CDS             173..1198
                     /locus_tag="ACHUOF_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0613307.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="ACHUOF_004436"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="ACHUOF_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAZOYF010000362         1799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZOYF010000362 DAZOYF010000000
VERSION     DAZOYF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.372"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            311..505
                     /locus_tag="ACHUOF_004437"
     CDS             311..505
                     /locus_tag="ACHUOF_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613308.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            866..994
                     /locus_tag="ACHUOF_004438"
     CDS             866..994
                     /locus_tag="ACHUOF_004438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613309.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(1304..1640)
                     /locus_tag="ACHUOF_004439"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="ACHUOF_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          546 a          395 c          379 g          479 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattataata acggcttta
//
LOCUS       DAZOYF010000363         1788 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZOYF010000363 DAZOYF010000000
VERSION     DAZOYF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.373"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..694
                     /locus_tag="ACHUOF_004440"
     CDS             <1..694
                     /locus_tag="ACHUOF_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR0613310.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..>1788)
                     /locus_tag="ACHUOF_004441"
     CDS             complement(1033..>1788)
                     /locus_tag="ACHUOF_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613311.1"
                     /translation="NALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVRLDAPR
                     LATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSFPYAGE
                     WLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRLVVKES
                     DHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLRIPDEP
                     PRRWFDRGVLREVVREARAEIRSMADALAKIRK"
BASE COUNT          453 a          488 c          464 g          383 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcatt
//
LOCUS       DAZOYF010000364         1775 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZOYF010000364 DAZOYF010000000
VERSION     DAZOYF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.374"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(1..279)
                     /locus_tag="ACHUOF_004442"
                     /pseudo
     CDS             complement(1..279)
                     /locus_tag="ACHUOF_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(394..1032)
                     /gene="ospI"
                     /locus_tag="ACHUOF_004443"
     CDS             complement(394..1032)
                     /gene="ospI"
                     /locus_tag="ACHUOF_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR0613312.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1179..1364)
                     /locus_tag="ACHUOF_004444"
     CDS             complement(1179..1364)
                     /locus_tag="ACHUOF_004444"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0613313.1"
                     /translation="MLPLSVNLMSSGASLAARLQQHWLWYSYNTKTGGVLAYTFGPRN
                     DETCRELLVLFTPFCIY"
     gene            complement(1389..1661)
                     /locus_tag="ACHUOF_004445"
     CDS             complement(1389..1661)
                     /locus_tag="ACHUOF_004445"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFR0613314.1"
                     /translation="MATVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKPGVKEQIVEMAHNGAGGRDTARTLKIGINTVIRTLKSSRPGG"
BASE COUNT          504 a          439 c          325 g          507 t
ORIGIN      
        1 gggggccagt ctacccagca agcgctgtaa ctcttttatt tgttttatag cggcagccag
       61 ttctgaggca ggaacgacat cctctccggc agcaacagca gtgagacttc cttccagata
      121 ttattttttc cacttaagca acaggctggg ctgaatacca tgcaaacgcg ccacatggga
      181 caccgtcatg ccaggctcca ttgtctgctg aataatggca attttttcct gaggagtttt
      241 acgtttacga agctccagcc ccagcaaagt tccagtcatc tcaaaattag aactagtgtt
      301 agacatatat ccaagcctat ctcttatttt aagatacatc cactgtctgg cgtttcaagg
      361 ggccggctca aatctatgta caggctctca gcatcagcaa agcctcttac ttttccaact
      421 actactgtta gcatagtgat caaattcctg atcagataca tgaattacct gaaccgaact
      481 attttcatct acaaatgcat ctggcgattt ttctacagga tcataaagat taaatccctt
      541 tcctgtttga acatccaaaa acataatttt ttcaccatcg ttaaatccag ctatccaatg
      601 agtggcctgt tcaatagaaa tcattactac acttcctttt gaagtatttt gtaaaatatt
      661 ttctgcaata cgctcacgaa atttactata agtttcatta ggatgtgaca ctatgccttc
      721 actatcaatt tgctctaaat gcctatgaaa aaatccggtc atatactctg atatttcatt
      781 aggtacggga gcctctctta ctccataccc taacatagcc atacaagcat gaagcgcaca
      841 ccctgaacag ttaccatccc caccagacgg atttatcatt ttctgtaaag tcgctgccaa
      901 ttcagaatct ttatccatat caccaagact tttagagttt aaagattcca gcaatttttt
      961 aacatttaca tttccctcct ctgtatgtgc ctcatatcca gcagttccct gtaacgacac
     1021 cccattaatc atattgtatc ctgacagtat atatactcac tatattttct tcataacaaa
     1081 tatccctctg atatgacaac aaaaaacgga gccggattcc tgtttttgtg aactgcagct
     1141 attcgtgtct aaaaagtgat ccacttacca ccaacggcct aatatataca gaacggagta
     1201 aacaggacta acagttcccg acaggtctca tcattccgtg gcccgaaagt gtaagccaac
     1261 acaccgccag ttttagtgtt gtacgaatac cagagccagt gttgttgcag ccttgctgcc
     1321 aacgaagcgc cactgctcat caagttcaca gataagggca acatcagcgt gagcgactgg
     1381 cgcagatgtt atccgccggg gcgggagctt tttaatgtcc gtatgacggt attgatgccg
     1441 attttcagtg tcctggcggt atcacggccc cctgcgccgt tatgtgccat ttcaacgatc
     1501 tgctccttaa cgccgggttt tctggcttca taagagtaag tgagctggaa aacacgttta
     1561 catgagcgac aacggaaacg ctcatgccgg gaacaactac ggccatgtcg ataaacttca
     1621 tcggaatgac agcgcggaca atgaacagta acggtagcca tggaaaacct taaataccag
     1681 tagattacca gaaacttctg tcgattgaag acatcacccg aagtttgaat tcattcgaat
     1741 actttttacg aggttctttt cgccagtcct gtaat
//
LOCUS       DAZOYF010000365         1748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZOYF010000365 DAZOYF010000000
VERSION     DAZOYF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.375"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            453..1301
                     /locus_tag="ACHUOF_004446"
     CDS             453..1301
                     /locus_tag="ACHUOF_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0613315.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1318..1599
                     /locus_tag="ACHUOF_004447"
                     /pseudo
     CDS             1318..1599
                     /locus_tag="ACHUOF_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
     gene            1635..>1748
                     /locus_tag="ACHUOF_004448"
     CDS             1635..>1748
                     /locus_tag="ACHUOF_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613316.1"
                     /translation="MAGIRSTLIKEKNMNTNYEATVATTDNIVHEVYLEGKH"
BASE COUNT          499 a          398 c          411 g          440 t
ORIGIN      
        1 gcattatata gttctactct ggcgagatac gttggcatat tcatttcctt actggttgtg
       61 tgagaacttc agtaaagata ccaccaaagc ccggaagtgg tgaaataaaa ccgggcacaa
      121 cacgaaggcg catttccggt atccataaag agtcggtctt gtctgtgaaa tttaaatggt
      181 gggagtgcgc ttccggttgt aaataacgac attgctgtgt gaagtaccag ttggcggcat
      241 cggtttaatt gctggctgat gtccgccctt tttaaagtga attttgtgat gcggtgaatg
      301 cggctatgcg cacgcggcac agttaaaagt catgttagtc cttattggtt tgggtgggaa
      361 agccaactgt aattgttaac tggttaacgt cacctggagg caccaggcac cgcatcaaca
      421 aagttcactt cggtgatgaa aggaaagaga aaatgttgaa tgtagctatt gaaaaccaga
      481 acgggtggaa ttatagtgca cctgcacctc ataaaacggg tgccgggatt gctaccccga
      541 cgatgactac tgcgcataac cgcgctcagg cggttttttt atgcgtcaag cacagccaca
      601 ttcagattat ggtggggcgt gcagggcagc cgcaaggctg gccggtttcg gtagtcaccg
      661 gttgtagcaa ccctgtgcgt ctcaccaccc atgagattgc tacctctggt ggtgagtcat
      721 ttaaactgac tatcgaggct gccatcatgg ctactatcct taccctttct caccctgacg
      781 caaccatcga aaatggtcgc gctgtcacta cgtctgttgc ggttgcggag ttcttccgga
      841 aaatgcacaa aaacgttatt cagaagatcg aaactctcga atgctcgccg gagtttaacc
      901 ggcttaattt taagcccgtc acctataccg acgccaaagg cgaaaaacgc ccaatgtacc
      961 aaatcaccaa aaacggcttc gttttcctgg tgatgggctt cactggcaaa aaagccgccg
     1021 cattcaagga agcctacatt gctgagttcg accgcatgga ggccgaactg cgccagaata
     1081 acaccccacc tgcagacaag atgattccgg gtgatggtcg cactctggtt gttcacttcg
     1141 acaaattcgg caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg gtctgtaccc
     1201 tggagacttt ccggttttat ctggaaaaac agggatggac tcttgttaac cggggcgcaa
     1261 ttaaaaatat gactgtggaa caattacttt ctttaaaata aaaatggagc tacggatatg
     1321 aaccctatgc ttgattattg ttttacctct gcacctcagc agaccataac acacaccgga
     1381 atcaaaaatt tgttatggat aatcaaaaaa aacctccagt agaaaggtga tcccgattta
     1441 attccctgtg cttatggcct gtgtgacaga caagtaagcc ctgataaggc ttacactgct
     1501 gaacttattt acatgcacgg aaccatgatg aggaaaaaga aacgcaaata ttgtagtgaa
     1561 atctgcgccg aaaaagacca gatggcacac gaactttaat taactgacta ttcgaaactg
     1621 aatttatgcc agcaatggca gggattcgct caaccttaat taaggagaaa aacatgaaca
     1681 ccaattatga agccactgtt gcaactactg ataacattgt tcacgaggtt tacctggaag
     1741 gaaagcat
//
LOCUS       DAZOYF010000366         1744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZOYF010000366 DAZOYF010000000
VERSION     DAZOYF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.376"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..353)
                     /locus_tag="ACHUOF_004449"
     CDS             complement(<1..353)
                     /locus_tag="ACHUOF_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR0613317.1"
                     /translation="MNRHTQIRQSVLACLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMA"
     gene            complement(350..940)
                     /locus_tag="ACHUOF_004450"
     CDS             complement(350..940)
                     /locus_tag="ACHUOF_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0613318.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(927..1298)
                     /locus_tag="ACHUOF_004451"
     CDS             complement(927..1298)
                     /locus_tag="ACHUOF_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR0613319.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHUOF_004452"
     CDS             complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHUOF_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR0613320.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          393 a          527 c          407 g          417 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtgggt tcagggtgtc gatgagtccg
       61 gaaagtgccg gtacatcatt cagggccggg aaaatggtgc tctccatcca catatccagc
      121 tctgaatccg gtgcctgtgc ccggatgaag acggcaatat gcagaacagc ctgccagtca
      181 tcttcatccg tcatttttcc ggtgtactga gcatcactca gccacaccgc cacggcaggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcatgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacagc gggattttca
      481 ctacctccac aggaagacga ttttttctta gcctgccctg atggtcatgc cctgttgcaa
      541 aacgcgcttc aggaagacac ctcagaacat gccagcggcc attcgccagt tgctgaacaa
      601 atgccccccg gaaaaagtat tttcccactc tcagtccttc accagcacgc cgccgtgttg
      661 tgttcagttt gatggcgggc ggattgcccc ggttaacgcg gatcctggcc gtcatttttc
      721 ctgacggact ggctttaaac acccggacac gctgacgtac cagtttcagg gggatccctt
      781 tcacctggtt atcgcccgca acggtattcc cggcaacctg ccgggtggca accgagaccg
      841 ctttctgtgc cacacggttt atcgcccatg cgctggcctg tggcaccata cgggtatcaa
      901 ggctgttcag attgcggatg gcattctcaa gccccttcat aatatcgtcc tgcccttacg
      961 tttcccggca gcccctcacg ccgcaaccgg atataactac atccgccatc atcaggggtg
     1021 atacgatcca cacagaaaga ctcacgacca atcatcagcg aatcattgcg acacagccgt
     1081 aaaatatctg ctgttttcac aaacagggat ggtaaagagt cttcaatacg cacaccgccg
     1141 gccaccagag aaatattttc aggatcatca aaaaccccgg aaatcatgat cccttttagc
     1201 cggcctgacg taatgaccgc tctaatcccc atattgcgaa taatggcctt gtctgcgaga
     1261 tcaagtgcaa catcaaacag attatcgaaa tcagccacgc tgcctcccgt tattgcattc
     1321 tggccaggcc acgttctgtc attccggctg ccacaccggc aaagacacgg aacgccattc
     1381 ccggcggcac aaatgccaga ggttcatccc gcgtggcatg aagtgcatcg gtatgcagcg
     1441 tcaccagtgc cacgactgta accagatcat ccatattctg aatcacggtc tccggctgcg
     1501 ctgatatcac ctcattttca tgtccggtca gcgcattttc cgggtgggca gacttatccc
     1561 ggatgtcagt gtcatccata ttggcaagtt cctcctccag ctcagccaca cgctgcgcaa
     1621 tatctgctgc gcttcccgag atatccggct cacgtcccag cgatgctgcc agttctctca
     1681 gacgtttcag gttatcgtct ttctttgcca tatctgttcc tgtccgaata aaaaacggga
     1741 gccc
//
LOCUS       DAZOYF010000367         1740 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZOYF010000367 DAZOYF010000000
VERSION     DAZOYF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.377"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..618
                     /gene="virK"
                     /locus_tag="ACHUOF_004453"
     CDS             <1..618
                     /gene="virK"
                     /locus_tag="ACHUOF_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR0613321.1"
                     /translation="PQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVI
                     ARLTFSVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMK
                     ACNISECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIR
                     KPLSDIASKKRSGYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            683..1627
                     /gene="lpxM"
                     /locus_tag="ACHUOF_004454"
                     /gene_synonym="msbB"
     CDS             683..1627
                     /gene="lpxM"
                     /locus_tag="ACHUOF_004454"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0613322.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          521 a          333 c          397 g          489 t
ORIGIN      
        1 ccacaaaaat cggtatcatt agtccaattc atcgggaaag atggagagaa ttttgatatc
       61 cagtgttctc ccagtggttt tgaccgggaa ggagagttga tgttgtcatt gttttacaat
      121 aaaacagtta ttgcccggtt aacattttca gtcattctga ctcaaaatgg acacattgct
      181 tttattggcg gattacaggg agcccctaaa aataccggac ctgatgttat tcggtgcgca
      241 acccgggcct gttatgggat tttcccaaaa cggattattt ttgaagcatt ttgtgcgttg
      301 atgaaagctt gcaatatctc tgagtgtctg gcagtcagtg agcacagtca tgttttccgg
      361 caattgagat actggtatca gaaacgcaaa acctttgttg cggtctatag tgatttctgg
      421 gagtctgtag caggaaaaac ctgtggtgac tggtacagat taccaaccca ggtgattcga
      481 aagccgctaa gcgatatagc cagtaaaaaa cgctctgggt accgaaaacg atacgctttg
      541 ctggattata tccatgaaac tactatccgc tctttggatg catatcctgt acactcagaa
      601 catcagaatt taaattaagg tttgatgtat tctctgaata aaacagtaat gataattttg
      661 gtagaggcat tcgcactaaa taatgaaaaa atataaatct gagtttattc ctgaatttaa
      721 gaaaaattac ctttcccctg tttactggtc tacatggttc cttttgggaa tgattgcagg
      781 tatttcaatg tttccccctt cattcagaga tcctgtcttg gcaaaaatag ggcgttgggc
      841 gggcagattg agcaaaaaag ctcgtcgcag ggcgacgatt aatttatcgc tttgtttccc
      901 agaaaagagt gatacagaac gggaaataat tgtcgacaaa atgtttgtca cagcattgca
      961 atccatagtg atgatggcag aactggcaat tcgtggtccg gaaaagttcc agaaacgagt
     1021 gttctggaaa ggcctcgaaa ttcttgagga aattcgacac aataatagaa atgtgatttt
     1081 tctggttccc catggctgga gcgtggatat tcctgcaatg ttgctggcag ctcagggaaa
     1141 aaaaatggcc gccatgtttc atcagcaacg aaatccagtg attgattatg tctggaattc
     1201 agtacggcgt aaattcgggg ggcgcttaca tgcccgggag gatgggataa aaccatttat
     1261 tcagtcagta cgccagggat actggggata ttacctccca gatcaggatc atggtcctga
     1321 atacagtgaa tttgctgatt tttttgcgac ctataaagcg acattaccaa tcattggacg
     1381 tctgatgaac atcagtcagg ctatgattat accacttttc ccggtttatg atgaaaaaaa
     1441 acatctactg actattgaaa ttcggccacc aatggatgct tgcattgcca gcgcggacaa
     1501 taaaacgatt gcccgacaaa tgaacaaaac agtggaaatt ttggtggggc cacatccgga
     1561 acagtatgtc tgggttttaa aattgttaaa aacacgcaaa tcaaacgaag cggacccgta
     1621 cccttgaaat tataaagtac aagtatttcc actagttgtt tgtaagcgta cagcctgaac
     1681 cgtctggtca gaatctgacg aattagacaa agtggtgtcc accaaataag tagtgggaac
//
LOCUS       DAZOYF010000368         1738 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZOYF010000368 DAZOYF010000000
VERSION     DAZOYF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.378"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            46..1146
                     /gene="ompC"
                     /locus_tag="ACHUOF_004455"
     CDS             46..1146
                     /gene="ompC"
                     /locus_tag="ACHUOF_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR0613323.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1310..>1738
                     /locus_tag="ACHUOF_004456"
     CDS             1310..>1738
                     /locus_tag="ACHUOF_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0613324.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDL"
BASE COUNT          533 a          353 c          355 g          497 t
ORIGIN      
        1 tcgtttagaa aatacgttta acgataatta aaaggattct gttaaatgaa attaaaaata
       61 gttgcggtgg ttgtaactgg tttgttagct gcgaacgtag cacacgctgc cgaagtctat
      121 aacaaggatg gtaataaact cgacctttat ggcaaggtta ccgctctacg ttattttact
      181 gatgataagc gtgacgatgg tgataaaact tatgcccgtc tcggctttaa aggagaaacg
      241 caaatcaatg atcaaatgat tggttttggt cactgggaat atgattttaa aggctataac
      301 gatgaagcca acggctcgcg cggcaacaag acccgtctgg cctatgctgg tttaaaaatt
      361 agtgaatttg gctctctgga ctatggtcgt aactacggtg tcggctacga cattggttca
      421 tggaccgata tgttgccaga atttggtggc gatacctgga gtcagaaaga tgtcttcatg
      481 acataccgta ccaccggtct agcaacctat cgcaactacg atttctttgg cttaattgaa
      541 gggctgaact ttgccgcgca atatcaaggc aaaaataaac gtactgacaa cagtcatctt
      601 tatggtgctg acgacacgcg tgccaacggt gacggtttcg gtatctcctc aacttatgtt
      661 tatgatggct ttggtatcgg tgcggtatat accaaatccg atcggacaaa tgcgcaggaa
      721 agagccgcag ctaatcctct caatgcctcc ggtaagaatg cagaactatg ggccacaggt
      781 ataaagtatg atgccaacaa catctacttt gcagctaatt acgatgaaac attaaacatg
      841 accacctatg gggatggtta tatctctaac aaagcacaaa gttttgaagt ggtggcgcaa
      901 tatcaattcg acttcggctt gcgcccatca ctcgcttacc tgaaatcgaa aggcagagat
      961 ctgggccgct acgccgacca ggacatgatt gagtatatcg acgttggtgc gacgtatttc
     1021 ttcaacaaaa atatgtcgac ctatgttgat tataaaatca acctgattga tgaaagcgac
     1081 tttacccgtg ccgtagatat tcgcaccgat aacatcgtcg ctacgggcat tacctatcag
     1141 ttctaacgtc ctctcctgac cagcccttct ctgggctggt ttttgattta agtgctgctg
     1201 tatgactcct taagtaaaaa tgagcaattc ttgatatttc tggtcattca cctcaattaa
     1261 tattattcca tatcctccgg ggacatctta ctataatatt tattgatgag tgattatgac
     1321 caatattaat acagcttgtg taaaaaataa tgccagttat caacttaata acgcattacc
     1381 taacaaagaa accatctcca gcaatttttg tgaacgacta gaacaatggg gtaataaatc
     1441 ggttaataat ggtgaagaaa gagcaattgc cgtggagcga attaaagaag cttacaattc
     1501 gaacatggca tctcttgatt tgtcatatct tgatttgagt gaattaccgc ctatcccctc
     1561 gacagttaat acgttaaatc tggaaaacaa ttgtctaact tgtcttgact tagctgacaa
     1621 tgccagtctc gttaatatca acctcagcgt taataaaatt aacacgataa cctttccaaa
     1681 agaaccaaaa ctggaaaaca tttatattaa tcataacaat ctggaaagtt tagattta
//
LOCUS       DAZOYF010000369         1731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZOYF010000369 DAZOYF010000000
VERSION     DAZOYF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.379"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..143
                     /locus_tag="ACHUOF_004457"
     CDS             <1..143
                     /locus_tag="ACHUOF_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0613325.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..486
                     /locus_tag="ACHUOF_004458"
     CDS             127..486
                     /locus_tag="ACHUOF_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613326.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLEN
                     VNITHWAVMPKAPEAGA"
     gene            486..791
                     /gene="yfdT"
                     /locus_tag="ACHUOF_004459"
     CDS             486..791
                     /gene="yfdT"
                     /locus_tag="ACHUOF_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFR0613327.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            707..1141
                     /locus_tag="ACHUOF_004460"
     CDS             707..1141
                     /locus_tag="ACHUOF_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0613328.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPLHTVQVSAGDGITEE
                     RKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            1063..>1731
                     /locus_tag="ACHUOF_004461"
     CDS             1063..>1731
                     /locus_tag="ACHUOF_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0613329.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESKIYSAMQKMTNRRHEE
                     NWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKV
                     PQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQINMQRRV
                     AWINP"
BASE COUNT          568 a          379 c          427 g          357 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cacgcaaaga
      901 tcccactaag aataaatctc cttacctcac aacacgacaa gcctgcattg cggctcttca
      961 gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg aaagaaaatg
     1021 tcactcttcc gcagaggtga aatatggtac gccagtttca cattgccgaa cggtaaaaga
     1081 tttaaacagt ctcttggaac aaaggacaaa aggcaggcga cagaactcca tgacaagcta
     1141 aaggctgaag catggcgggt cagcaaactt ggtgaaatac ctgatataac gttcgaggaa
     1201 gcgtgtgtca ggtggcttga agagaaagca cataaaaaat cactggacga tgacaaaagc
     1261 cggatcggat tctggcttca acatttcgca ggaatacaac taagagacat tactgaatca
     1321 aaaatttatt cagcaatgca gaaaatgacg aaccggcgtc atgaggaaaa ctggaaactc
     1381 agggcagaat catgcagaaa aaaagggaaa cctgttccag aatacacgcc aaaaccagcg
     1441 tccgttgcaa cgaaggctac gcatctttca tttataaagg ccctactaag agccgcagag
     1501 cgtgaatgga aaatgctgga taaggcacca attattaaag tgcctcaacc aaagaataaa
     1561 cggatccgct ggctggagcc ccatgaagca caaaggctga ttgatgaatg tccggagcca
     1621 ttaaagtctg ttgttgaatt tgcactggca acaggcttaa gacgctcgaa catcatcaac
     1681 cttgaatggc aacaaataaa tatgcagcgc cgggtggcat ggataaaccc g
//
LOCUS       DAZOYF010000370         1712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZOYF010000370 DAZOYF010000000
VERSION     DAZOYF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.380"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..263
                     /gene="yobA"
                     /locus_tag="ACHUOF_004462"
     CDS             <1..263
                     /gene="yobA"
                     /locus_tag="ACHUOF_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR0613330.1"
                     /translation="AQVTAAPQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNE
                     QDQKQLIVPLADSLKPGTYTVDWHVVSVDGHKTKGHYTFSVK"
     gene            267..1139
                     /gene="copD"
                     /locus_tag="ACHUOF_004463"
                     /pseudo
     CDS             267..1139
                     /gene="copD"
                     /locus_tag="ACHUOF_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            1152..1493
                     /locus_tag="ACHUOF_004464"
     CDS             1152..1493
                     /locus_tag="ACHUOF_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR0613331.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
BASE COUNT          414 a          400 c          444 g          454 t
ORIGIN      
        1 acgcgcaagt gacagctgcc ccgcaggcaa tcactttaaa cttctcggaa ggcgttgaaa
       61 caggattcag tggtgcaaaa atcacggggc caaaaaacga aaatattaaa acattaccag
      121 cgaagcggaa tgagcaggac caaaagcaac tgattgttcc actggccgat tcgctgaaac
      181 ccggaaccta caccgttgac tggcatgttg tttcggtgga cgggcataaa acgaaaggac
      241 actacacctt tagcgtgaaa taaatcatgc tggcgtttac ctggatcgcg ctgcgattta
      301 tccatttcac ctcgttgatg ctggtctttg gctttgcaat gtatggtgcc tggctggccc
      361 ccttaatgat tcgtcgcttg ctgacgaagc gttctctgcg tttacaacag cacgctgccg
      421 tctggagttt gatcagtgcc actgcaatgc tcgccgttca gggcgggttg atgggaacgg
      481 gatggacaga tgtattttcg ccaaacattt ggcaggctgt tttgcaaacg cagtttggtg
      541 gcgtctggtt atggcaaatt gttctcgccc tcgtaacgtt gatcgtcgcc cttatgcaac
      601 cgcggaatat gccacgtttg ctgtttatgc tcactaccgc gcaatttatt ctgctggcgg
      661 gagtggggta tgcgacgctg aatgaagggg taacagcgaa aatccatcag actaatcacg
      721 cgattcacct gatttgtgcg gcggcgtggt ttgggggatt actgccggtg ctctggtgta
      781 tgcaactcat caaaggccgt tggcgacact aggctattca ggcgctgatg cgtttttcct
      841 ggtgcgggca ttttgcggtg atcggcgtac tggcaagcgg cgtgcttaat gcattgttga
      901 ttaccggatt tccccccacg cttacgactt actggggcca actcttattg ctcaaagcca
      961 tcctggtgat gattatggtg gtcattgcgc tggctaatcg ttatgttctt gtaccgcgca
     1021 tgaggcagga tgaagatcgt gcggcaccct ggtttgtgtg gatgactaaa ctcgaatggg
     1081 cgataggtgc ggtagtgctg gtgatcatca gcctgcttgc gactctcgaa cctttttgat
     1141 ggattacgaa aatgatgaaa aaaagtattc tggcgtttct gttactcacc agttctgcag
     1201 cggcgctggc ggctccacag gtgataaccg tgagtcgttt tgaagtgggt aaagacaaat
     1261 gggcgtttaa tcgcgaagag gtaatgctga cttgccgacc gggtaatgct ttgtatgtca
     1321 tcaacccaag taccctcgtg cagtatcctt taaacgatat cgcacaaaag gaagttgcca
     1381 gtgggaagac caatgcccaa cccatttcgg tgattcagat tgatgatcct aacaatcccg
     1441 gcgaaaaaat gagtctggca ccgtttatag aacgagctga aaaactctgt taattaccta
     1501 aaatagcctt ttgatttcca ataaaaaaac cgcctcagtt ctttcaccag aacgggcggt
     1561 ttttaacatt taagctgatg agcaccgcgc tttttattga ccattttgca cgcaaactgg
     1621 aaaacctggc gtcgtcatct attcttaaag ggcaaggcaa ctaagcctgc attaatgcca
     1681 acttttagcg cacggctctc tcccaagagc ca
//
LOCUS       DAZOYF010000371         1697 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZOYF010000371 DAZOYF010000000
VERSION     DAZOYF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.381"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>1697
                     /locus_tag="ACHUOF_004465"
     CDS             <1..>1697
                     /locus_tag="ACHUOF_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HFR0613332.1"
                     /translation="AAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPEGLDRKF
                     YEICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQ
                     YLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQL
                     LPRIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESS
                     PGLTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGG
                     RGESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLR
                     IEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLIGGTL
                     NIKHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFI
                     LDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTA
                     AIVLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDG"
BASE COUNT          320 a          582 c          532 g          263 t
ORIGIN      
        1 ggcagccgtg cagaccctgc gtgagatgaa cgccgacaac ctgcgcaagg tgccggccga
       61 tgcacccacg gccttcatca agccgcgctg gaagccgctg gtgatcaccc cggaaggcct
      121 cgaccggaaa ttctacgaaa tctgcgccct gtccgagctg aagaacgccc tgcgctccgg
      181 cgacatctgg gtcaagggct cgcggcagtt ccgcgacttc gacgactacc tgctgccggc
      241 cgagaagttc gccgcactca agcgcgagca ggccctgccc ctggcgatca acccgaacag
      301 cgaccagtac ctggaagagc gtttgcagct gctggacgag cagttggcca ccgtcacccg
      361 cctggccaag gacaacgagc tgcccgatgc catcctcacc gagtcagggc tgaaaatcac
      421 cccgctggat gcggcggtgc cggatcgggc gcaggcgctg atcgaccaaa ccagccagtt
      481 actgccgcgc atcaagatca ccgaactgct gatggacgtg gacgactgga cgggcttcag
      541 ccgccacttc acccacttga aggacggggc cgaggccaaa gacaggacgt tgctgctgtc
      601 cgcaatcctc ggtgatgcga tcaacctcgg gctgaccaag atggccgagt cgagccccgg
      661 cctgacctac gccaagctgt cctggctgca agcctggcac atccgcgacg aaacctattc
      721 ggcggccttg gccgagctgg tcaaccacca gtatcgccac gcctttgccg cccactgggg
      781 cgacggcacg acctcatcct ccgatggcca gcgcttccgc gcgggtggcc ggggcgagag
      841 caccgggcac gtcaacccga agtacggtag cgagccggga cggctgttct atacccatat
      901 ctccgaccag tacgcgccgt tcagcacccg cgtggtgaat gtcggcgtcc gcgattccac
      961 ctatgtgctc gacggcctgc tgtaccacga gtccgacctg cggatcgagg agcactacac
     1021 cgacacggcc ggcttcaccg atcacgtctt tgccctgatg cacctgctag gcttccgctt
     1081 cgcgccgcgc atccgcgacc tcggcgaaac caagctgtac gtgccgcagg gcgtgcaagc
     1141 ctacccgacg ttgcgcccgc tgatcggcgg caccctgaac atcaagcacg tgcgtgccca
     1201 ctgggacgac atcctgcgcc tggccagctc gatcaagcag ggcaccgtca ccgcctcgct
     1261 gatgctgcgc aagctcggca gctacccgcg ccagaacgga ctggccgtgg ccctgcgcga
     1321 gctgggccgg atcgagcgca cgctgttcat cctggactgg ctgcaaagtg ttgaactgcg
     1381 ccgccgcgtg catgccggcc tgaacaaagg tgaggcgcgc aactcgctgg ccagggcggt
     1441 gttcttcaac cgccttgggg aaatcaggga tcggagcttc gagcagcagc gctaccgggc
     1501 cagcggcctc aacctggtga cggcggctat cgtgctgtgg aacacggtgt acctggaacg
     1561 cgccacccag gggttggtcg aggccggcaa gccggtggac ggcgagctgc tgcaattcct
     1621 gtcgccgctg ggctgggagc acatcaacct aaccggcgat tacgtctggc ggcagagccg
     1681 cagactggaa gacggga
//
LOCUS       DAZOYF010000372         1693 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZOYF010000372 DAZOYF010000000
VERSION     DAZOYF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.382"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..357
                     /locus_tag="ACHUOF_004466"
     CDS             <1..357
                     /locus_tag="ACHUOF_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR0613333.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            619..981
                     /locus_tag="ACHUOF_004467"
     CDS             619..981
                     /locus_tag="ACHUOF_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFR0613334.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            978..>1693
                     /locus_tag="ACHUOF_004468"
     CDS             978..>1693
                     /locus_tag="ACHUOF_004468"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR0613335.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVAS"
BASE COUNT          476 a          354 c          398 g          465 t
ORIGIN      
        1 gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa caaaaacagc agcggtaagg
       61 aagatgagaa ccgacagcaa caaggcatgg aagggagcgt taaagcgggc aggcattagc
      121 aacttccgct tccatgacct gaggcatacc tgggcaagct ggctggttca gtccggtgtc
      181 tctcttcttg cgcttaaaga gatgggagga tgggaaactc tcgaaatggt tcaaagatac
      241 gcccaccttt cagccgggca tcttaccgag cacgcgagca aaatcgatgc gattataagt
      301 cgcaatggca caaatacggc acaagaggag aacgtagttt acttaaatgc gaggtaactt
      361 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaattat
      421 aagaatccgc ttgtaattca aaggattatc ccatcaacac tgcgctcaca cgtcccacca
      481 catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct tatgtgtctc agttttgtct
      541 catcagacat agcaagtatc gatcaattga gacttggatg atagacttca tgcctttcag
      601 agctcattga ttaaataaat gttaaagtta tttgtaaagt acacatctat aggtgtgctg
      661 aacacactta tacactgggt ggtttttggt gtatgtatct atgccgcaca taccagtcag
      721 gctctggcaa acttcacagg tttcgtagtg gctgtgagct ttagcttctt cgcgaatgca
      781 agattcacat tcaaagcatt gactacagcg atgcgctaca tgttatacgt gggatttatg
      841 ggaatattga gtgtgattgt tggttgggca gctgataaat gctcacttcc tccaatagtc
      901 actcttatca ccttctccgc catcagtctg gtgtgcggtt tcgtctattc aaagttcatt
      961 gtctttaggg atgcgaaatg aaaatatctc ttgtcgttcc tgtcttcaat gaagaagaag
     1021 cgatacctgt tttctataaa acggtacgtg aattccaaga gttgaagcca tatgaagtag
     1081 aaattgtatt cataaatgac ggaagtaaag atgccacaga gtcaattatt aacgcgctgg
     1141 ctgtttcaga cccgctagtt gttccgctgt catttacacg caactttggt aaagaaccag
     1201 ccttatttgc agggttagac catgcaagcg gcgatgctgt aattcctatt gatgtcgacc
     1261 tgcaagaccc aattgaggtt atccctcatc ttattgaaaa gtggcaggca ggtgctgaca
     1321 tggtgcttgc taaacgttca gaccgctcaa ctgatggacg actgaaacgt aagacagctg
     1381 agtggttcta taaattacac aacaaaataa gcaccccaaa gatcgaggaa aatgtcggag
     1441 attttcgact catgtctcgt gaggttgtgg agaacattaa actgttgcct gagcgcaatc
     1501 ttttcatgaa aggcatactg agctgggtgg gtggtccgac ggatgtcgtt gaatatgtac
     1561 gcgcagagcg tgttgctggc atctcaaaat ttaatggctg gaaattatgg aatctggcac
     1621 tggaaggtat cacaagtttt tcaacctttc ctcttcgcgt atggacttat ataggcttgt
     1681 ttgttgcaag cat
//
LOCUS       DAZOYF010000373         1670 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZOYF010000373 DAZOYF010000000
VERSION     DAZOYF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.383"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(72..148)
                     /locus_tag="ACHUOF_004469"
     tRNA            complement(72..148)
                     /locus_tag="ACHUOF_004469"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Arg,seq:tct)
     gene            complement(150..225)
                     /locus_tag="ACHUOF_004470"
     tRNA            complement(150..225)
                     /locus_tag="ACHUOF_004470"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(190..192),aa:Ile,seq:cat)
     gene            complement(402..1091)
                     /locus_tag="ACHUOF_004471"
     CDS             complement(402..1091)
                     /locus_tag="ACHUOF_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFR0613336.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1088..1447)
                     /locus_tag="ACHUOF_004472"
     CDS             complement(1088..1447)
                     /locus_tag="ACHUOF_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0613337.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(1447..1635)
                     /locus_tag="ACHUOF_004473"
     CDS             complement(1447..1635)
                     /locus_tag="ACHUOF_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613338.1"
                     /translation="MPFYFRKECPLNSGYLNMVPALRELMQAKHRAQKAVDELVDCVA
                     ELETRVGKFEKLVQEVLR"
BASE COUNT          407 a          468 c          356 g          439 t
ORIGIN      
        1 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctctg cctgctgagc
       61 taagtcggcg ctggcccacc accgaggact cgaacctcgg accgtcaact tagaaggttg
      121 atgctctatc cggatgagct agtggtggtt ggtggccctt gctggacttg aaccagcgac
      181 ctggcgatta tgagtcgctc gctctcacca ctgagctaaa gggccggagg cagaataata
      241 accatatgtc atcacatctg caaactcatc tgaccaccag cacgtttaac gtcctgtgcc
      301 atttttcagg cataaaaaaa ccgctcggcg gcgggttttg cttactttac catcgcgtac
      361 aaaatcggca aagtatcaga tttgcatgag atatatgcct tttaatctac ttttgcaata
      421 ctttgctttg aaaatgccgc tttttgttct gaacgtgttc tcatcacaaa caataaagcc
      481 tcactatcca gtcggtgaaa aatgtgtttc attacaaccc agtgaccagt aaatgttttg
      541 gaccagtttt tggttgttac tcccgccagt aatgccagct cctgatattc ataaccttcc
      601 ccaccaaaaa gttctgcttt taccgcctgc gccgccagcc agattaattt tttcaggcgt
      661 tcctgtgttt tctctgcaat tttcctagtg ccagaatgag ccttaaactc ttcccacgcc
      721 cagtgggtta tggtgatctg atactcccag caaatacttc cgctgtaaca ccacaacaac
      781 caggctttat agtgctcttc aagagacaga acagcccgcc gccacgatga tgtcgaaaac
      841 gcaacctgac tgaccagagg aattgacgcc cccttcgcca gtgattgttt tcccgggatt
      901 ggtggattat cccgcgttat catttttccg gtcacctcat cgcggtaccg gattttttta
      961 cgcctgtaac gccctgtatc gaacatggca ttctcctgcc aggcttcaag ctgacctttt
     1021 gtcgccccac tcaaatcagc ggtggcgata atgagctgct cacgaacaaa ctgtaaatac
     1081 tggttattca tgcacactcc agctctgtga tttttatccc caaccgacca ccaggaacag
     1141 gcagtccgcg cacaatattg gtttcatcaa actgctcgtc gtcgataagc aaccccgcat
     1201 gtgtcagtgc atccagtggt gctttcagga tattgtccag gtcacgacgc cgcttatccg
     1261 gtggctctgc aataatcttt atcgccagtc ttccggacag gcttaatttc agtcgctgct
     1321 gacggacaat aagcgccaca tcccggcgat aacgctcacc ggcttttgat acaaaatatg
     1381 tgctgccacg acgtcgccag taagtattca ccgtcggcgg ataaggtaaa ataaactcat
     1441 ggcgcatcag cgcagcacct cctgcaccag cttttcaaac tttccgactc tggtttccag
     1501 ctctgccaca cagtccacca gctcatctac tgctttctgt gcgcgatgct tcgcctgcat
     1561 cagttcccga agcgcgggta ccatattgag gtagcctgag tttaacggac actccttcct
     1621 gaaatagaat ggcatcagaa ggagccaata atgagcagaa aaacccaacg
//
LOCUS       DAZOYF010000374         1647 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZOYF010000374 DAZOYF010000000
VERSION     DAZOYF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.384"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..149)
                     /locus_tag="ACHUOF_004474"
     CDS             complement(<1..149)
                     /locus_tag="ACHUOF_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613339.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFW"
     gene            421..723
                     /locus_tag="ACHUOF_004475"
     CDS             421..723
                     /locus_tag="ACHUOF_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR0613340.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            714..1196
                     /locus_tag="ACHUOF_004476"
     CDS             714..1196
                     /locus_tag="ACHUOF_004476"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0613341.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            1264..1647
                     /locus_tag="ACHUOF_004477"
                     /pseudo
     CDS             1264..1647
                     /locus_tag="ACHUOF_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          429 a          350 c          413 g          455 t
ORIGIN      
        1 tcccagaaac agaccgtgca ggagggatga ccggtgataa agtctgcaaa tacggactta
       61 taagggtgat tattggctct ggcgacggct ttcagaacct ctttcaccat tctggtgtgg
      121 actttctggt gctccaggtt gtgtgacatg ggaactcatt ctggatggtt actctgaaag
      181 ctcatattct gccacacccc gatttgcagc cgccaggctg ccgtggttca ggcatttcat
      241 tatcaaaatc acattaaacg gccgtaatca gacatgattt gtgcgccgac acagatcatc
      301 gtcacaatgt tcaggtctct gccattgttt ttatcagggg atcgcttttc ccgtaagtga
      361 ttctgtatag cgttctgtta ccatttgcga taacattaat tgcatagcaa attgttacca
      421 atggaggttt ttatgtctac agctgcaagc gtccgtaaaa caccgcgaga acatcagatt
      481 aacatccggg caactgatga ggaacgagcc gttattgatt atgctgcgag cttggtcaat
      541 aagaaccgta ccgatttcat catggaactg gcgtaccagg aagctaaaaa cattatcctg
      601 gatcaacgtt tatttgtgct ggataacgaa cgttatgatt cgtttattac gcaactggaa
      661 gctcctgtgc agaatgcaga agggcgtgaa cgtctgatgg cggtaaagcc tgaatggaaa
      721 taaatgtcac cgcgccagca ttgttgacgg atgagcatat acttcagcca tttgactgtg
      781 gaaatgaggt gctaagtaac tggttacgtg gtcgggctat gaaaaaccag atgctcaatg
      841 cttctcgcac gttcgttatt tgcttggagg atactttacg tattgtggga tactactcat
      901 tagctactgg ttccgtcact catgccgagc ttggtcgcag tttgcggcat aacatgccca
      961 atcctgtccc tgttgttctg ttagggcgtc tggctgttga tgtttgtacc caggggcatg
     1021 gtttcggtaa atggctacta agtgatgcca ttcaccgggt tgtcaatctg gctgatcagg
     1081 ttggcattaa ggctgttatg gtacatgcaa ttgatgatga cgccagagca ttttatgagc
     1141 gttttggttt tgttcagtca gttgtggcac ctaatacctt gttttataaa gtctgagtct
     1201 gaggtagcct gagtttaacg gacactcctt cctgaaatag aatggcatca gaaggagcca
     1261 ataatgagca gaaaaaccca acgttactct acggagttta aagccgaagc cgtcaaaacg
     1321 gttcctgaaa atcagctttc gatcagtgaa ggcgcttccc gattatctgt tcctgaaggc
     1381 actttaggac aatgggttac cgccgcaaga aaagggctca atacaccagg ctcccgcaca
     1441 gtggctgagc tggaatctga agttatgcaa ctgcgtaagg ctttaaatga agcacgactt
     1501 gagcgagata tattaaaaaa agcaacagcg tattttgcac aggagtcgct gaaaaatacg
     1561 cgttagtcga acaatggcga caacaattcc ctgttgaagc gatgtgtcgg gtacctggtg
     1621 tatccaggag cggttattac gaccggg
//
LOCUS       DAZOYF010000375         1603 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZOYF010000375 DAZOYF010000000
VERSION     DAZOYF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.385"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(335..481)
                     /locus_tag="ACHUOF_004478"
                     /pseudo
     CDS             complement(335..481)
                     /locus_tag="ACHUOF_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            535..717
                     /locus_tag="ACHUOF_004479"
     CDS             535..717
                     /locus_tag="ACHUOF_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR0613342.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(818..1147)
                     /locus_tag="ACHUOF_004480"
     CDS             complement(818..1147)
                     /locus_tag="ACHUOF_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR0613343.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1319..>1603)
                     /locus_tag="ACHUOF_004481"
     CDS             complement(1319..>1603)
                     /locus_tag="ACHUOF_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613344.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          436 a          368 c          377 g          422 t
ORIGIN      
        1 atagcaatgt ctgtcgcaca acaaccacaa tgtcacaaat aaaaaatgag aataaccagt
       61 tccctgaagg cgacgtagcc cggtacaaga gtaacccacg ttagcgcttg gcgacggcgg
      121 aacaaagctg attcgcgacg taagaaagtg gcaggctcat atgacggacc tctgtaatgc
      181 ggtatccaac ccgcgaatat cagcatgatt caccgtcgga tttactgcta cgtcgccctc
      241 agggaattaa acctaaaaag aaaatcgtca aaattagaga ttccggtttg acacggaaag
      301 tcatatcagc gtcaacggag caccgtattg acgcttattt attggtgaga actacgttcc
      361 atggcaggag ttcgtcaaca cggttggagg gccattcagg cagtatgctc agaatatggc
      421 gcagatacgc ttccggatcg ataccgttca gacggcaggt gcctgctttt ccggcaacaa
      481 gcaggaatct cagcgctgaa gcaggaatta gaggtaaaaa cgccctaccg ggccatgaat
      541 cacccggtca ttggagtagt aaccaaagca gatttagcca gcatggaaca aatatctttg
      601 gtgaaaagtt ggttacggga agctggagcg cacaacgtgt tagtaaccag tgcagttaat
      661 aacaatggag ttacagaact ttttgccttg ctgcatacag aagaaggctg ttgttaattt
      721 cgattggttc tcagatgaga atgatgacgg tgatacacag ataagaatca atgagcaatg
      781 agggttgccg ggcaaccctc attgaataaa acgggaatta cttcgcttcg ccgtttttca
      841 tttcgcccat agctttcagc tttttggaga tatcgcggcg ttctttggag agctcggcat
      901 ttttgatgat gtaatcgtca acgcgatctt catagtcacc tttcatgctg gcgatgatgc
      961 cctggattgc ttcaacgctc atccctggct tgatgtaatc gctcaggttg tccagcaaca
     1021 ggacgcgctt ctggttgtca cggatctttt tctcaacgtc ctgaatttca cgttgcagtt
     1081 tgttcttacg acggaacaga cgaacaaatt ccagtacgtc ctggaatgaa ggcttggtag
     1141 tttccatttt tatacccctg ataatgtgag agtcggattc gtttaagcaa ccgctattcg
     1201 ttagggccaa cattactcat agctgccgca gatgacaatg cttttatccc ttttcactat
     1261 catacccttt atccttgctg aatcgaagca gcagcaagat gattctgaag ttcaggaatt
     1321 atttgcgcaa ggcccggcaa atcagattcg atagcaactc aagctgatgt gccgtttcca
     1381 tgttcagcca cacataacca tagattggtg tttccacgac tttcaggtca tggtggttat
     1441 cgagcaactg acgcagctct tccacagcat cgttcaattt ttccgtattg gcaaaaaccg
     1501 gctgcggatt accttcgtac agcgcatgaa caaggctcag cagtatttgc tgcatcatgt
     1561 gctgggtatc acaaagtttt tgcgcgttca ataacacgaa atg
//
LOCUS       DAZOYF010000376         1581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZOYF010000376 DAZOYF010000000
VERSION     DAZOYF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.386"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHUOF_004482"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHUOF_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFR0613345.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1581)
                     /locus_tag="ACHUOF_004483"
     CDS             complement(1269..>1581)
                     /locus_tag="ACHUOF_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFR0613346.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          482 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac a
//
LOCUS       DAZOYF010000377         1563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZOYF010000377 DAZOYF010000000
VERSION     DAZOYF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.387"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            109..>1563
                     /locus_tag="ACHUOF_004484"
     CDS             109..>1563
                     /locus_tag="ACHUOF_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0613347.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYS"
BASE COUNT          538 a          218 c          284 g          523 t
ORIGIN      
        1 tgcttcagta tttcgttaat taatataaat acaaaaggcg ttgtgctttt ataattataa
       61 agcgcgagga gttcggtgga ttatcgtttt tcttgggtga tttttaatat gataaacaat
      121 atatctgatc aagcatcatc atttccaggc acacaactaa atcaaagtga taatttcctg
      181 gattcactga gggaattttt cgcgatatta aatcccagtc gtaagggtga gttgtctaca
      241 tgggatacca tctacttgca tctgatatta gcaataaatg ctgacagtga tctcataaaa
      301 aatgatgtgt tgctcgcgga aaatattcca tcagcgaatt accaatttaa tacatttttt
      361 agcaatactt tcgagattga tgttaaaaaa tatctgggta aatcagaaga taacgaagtt
      421 gaaataaaag cgggtaatga aagaatctct attggaatac gtaatgtatc gaatggaaaa
      481 cttgaaagac aacagtttct tttcccactg gactatgaga ataaattaca agaacaatta
      541 gataagtatt ttacgattga atcgcaccca ttattataca ggtataccat tggtagtaag
      601 attgcgaatg tgatttttga aaagttatac agtcgtattg actttaacaa agaacaatat
      661 atatccttta taaaggacgc atttattcat ttttatgatt atagcaggcg atatgctatt
      721 agtgagaata tcgataaaga cgctgtaaca aacaatatcg cattgatgag cactttttat
      781 gacagtgata atacgtcagg tgaggtttta aataatgatt ttacagaaga agagtcattt
      841 gaaactgcgt tagatgtaga gcatgcaata gtgcttggat ttgctgatga taattttgaa
      901 acaaaacctg ttcattatca ggatttatta acacgattta gtgcctttca agataccgtg
      961 tttaatctgt ttcctgaaat gcactcttcg cattaccacg atatttgtag cgtaagtgtg
     1021 gatatgacaa agggaacgca atgcatgatt cacttgatgg tgaatgaaga agttttcatg
     1081 tctctgcctg ttccagttgc cacgatggta cgtgaagatg caagtaattt agtcaatctt
     1141 aaaacactac tcaatgatgg ttgtttcatt aaatatagtc attttaatga cgtggcatta
     1201 ataaagcaaa acataagtaa tctctattta tcacacactg ttgtgaatga aagcatattg
     1261 aaaaaatgtt gttttgaaaa cggcagttta ggtgatgtta aaataacaaa tagcaatgta
     1321 ataaacagtg cattcaaaaa tatttcgttt agatctgtta aaattaataa cgtaaatact
     1381 cattctttaa aatttattaa ctgcaatttt ttcaatgttg atatgataag agttaatctc
     1441 agcaaatgtc tttttcatga atgtagtatg catggtgtca agataaaacc ctggttgcct
     1501 gtaaaatgga caaaagaatt aataagtgat tatttgtatg gctgtttact atctctgtac
     1561 tct
//
LOCUS       DAZOYF010000378         1556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZOYF010000378 DAZOYF010000000
VERSION     DAZOYF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.388"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(77..730)
                     /gene="ribB"
                     /locus_tag="ACHUOF_004485"
     CDS             complement(77..730)
                     /gene="ribB"
                     /locus_tag="ACHUOF_004485"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR0613348.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(834..982)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1104..1394
                     /gene="ubiK"
                     /locus_tag="ACHUOF_004486"
     CDS             1104..1394
                     /gene="ubiK"
                     /locus_tag="ACHUOF_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR0613349.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT          438 a          399 c          344 g          375 t
ORIGIN      
        1 atagcagggc aaaataaaaa ccgaagccag ctacaacttc ggtttcttga ttaaggcagt
       61 aattaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat gccaccaggt
      121 cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac tctggtgcac
      181 gcgccatcgt gccatcgtca ttagtcagct cacacagcac accagccggt ttaaagcctg
      241 ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg
      301 cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag atcagaaggt tttgcgccat
      361 cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg gtagtcacac
      421 cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg ttattttcta
      481 ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg caaacaatac
      541 cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct gccgggaaga
      601 tcatatcgcc ttcgttttca cggtcttcat catcaagcac cattacaccg cgtccttcac
      661 gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg
      721 tctgattcat ggtaaaaaaa cctcactaaa attatggtta ccagaatcag ggcagtctta
      781 ggagtggcgg catatagcca aaataacgtg agcgggtcca tgcccgacag aatcgttact
      841 ctctcccatc cggactctaa ccgtcggccc cggaattaca ccggatctgc tgtcctttga
      901 gttcgcaccc aaagcgctcg cgggctttca actgagttga tttaccgccg gtggggaatt
      961 tcgccccgcc ctgagaataa gcggattcac tataacgcta atgattagcg gcagcaacgc
     1021 atagcttcac ataattctgg tttatgactt acccttatcg cactacaatg gtactcaaca
     1081 cctatcagta cagggaaact acaatgattg acccgaaaaa aattgagcaa atcgctcgcc
     1141 aggttcacga atcaatgcct aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa
     1201 tccgccaaac cctacaagct cagctgacgc gcctcgacct tgtaagccgt gaagagttcg
     1261 acgtccaaac gcaagtcctg ttacggactc gtgagaaact ggcgctgctt gaacaaagaa
     1321 tcagcgaact ggaaaatcgc agtactgaga tcaaaaagca gccagatcca gagactctcc
     1381 ccccaacgct gtaaacattt gtctttaatg cactgtaaat tatgaattaa tcctctaatt
     1441 tacagtgttt tctattacat tatcgcttaa tcccattcag agtatcatca caaaaatgaa
     1501 tgattgcaat tgaaaatata attgttatgt attctttaag ttttacttaa tcgata
//
LOCUS       DAZOYF010000379         1525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZOYF010000379 DAZOYF010000000
VERSION     DAZOYF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.389"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            71..352
                     /locus_tag="ACHUOF_004487"
     CDS             71..352
                     /locus_tag="ACHUOF_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR0613350.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            318..1395
                     /locus_tag="ACHUOF_004488"
                     /pseudo
     CDS             318..1395
                     /locus_tag="ACHUOF_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          525 a          299 c          341 g          360 t
ORIGIN      
        1 tattaaaaat caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag
       61 aggtatggca atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa
      121 cccaccatca atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc
      181 tgaactacac gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa
      241 ggttgatacc gatataacac accatcagcc taatgggcga caagggagag acagtccgtt
      301 aatggagaag ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa
      361 catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta
      421 cactaggtaa aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata
      481 tatacaaaaa ctactctcct gctgccctaa ttgaaaagct taaaggggtc gactcattta
      541 ctatggcagg ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gcgtccagaa
      601 atacttataa aactcgggta aatcaactgg agacaataaa agaaaaatta ggaggggttt
      661 tactgacaga aataaccact cgccatatcg ccgagtttct tgatttgtag attgaaggag
      721 ggaaaaacac gatggcagga tcaatgcgtt ctgtgttatc tgatatgttc cgcgaagcca
      781 ttgttgaagg acgtatatct caaaatccag taacgccaac aagagcaccg aaaatagtag
      841 ttacaagaga acggctgaaa ctaaagacat acaactgcat cagggaggca gcagatcaac
      901 ttccggcatg gttcccatta gctatggact tagcccttgt aacaggacaa cgtcgcgaag
      961 acataacgaa tatgcggttc agtgatattt atgatgatcg tctccacatc aggcaaatta
     1021 agacaggaat gatgattgct atccccctgt cactcagcct tcctgtcgct ggtttacgac
     1081 ttggtgcagt agttgaacag tgccgcatgg taagtagggg ggattatcta atcagtgccg
     1141 ggattagaaa aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg
     1201 tcgcagccag aaaattaaca ggtatcactg ttcagtgaaa acccaccaac ttttcacgaa
     1261 atcagaagcc tggctggacg attgtacaaa gaaatatgtg gagaagaatt tgctcagcgt
     1321 ctacttggcc acacatcgga gaagacaaca aaaatgtatc ttgatgagag agaaaaaacg
     1381 tacttactgc tctgatttta acgtaaatgg attgttaaat gtattttggt tgtgatataa
     1441 ccaaaaaaga ccggaataca gaaattcgag taaatttcgg ggaatttcgg ggagacgttt
     1501 gcaactaatt gattttaaat acaat
//
LOCUS       DAZOYF010000380         1489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZOYF010000380 DAZOYF010000000
VERSION     DAZOYF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.390"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            41..1438
                     /gene="ccp"
                     /locus_tag="ACHUOF_004489"
     CDS             41..1438
                     /gene="ccp"
                     /locus_tag="ACHUOF_004489"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR0613351.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT          401 a          309 c          405 g          374 t
ORIGIN      
        1 tttctgggtc acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat
       61 taccgcgatc ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg
      121 gtaccacgat aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa
      181 taataaggtt ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc
      241 agaattaccc gcctattatt atattcctgg cgcgaaacag ttgatggatt acgacattaa
      301 gcttggatat aaatctttta acctcgaggc cgtgcgtgcg gcactgctgg ctgataaacc
      361 cgtttcgcaa agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccacc
      421 aacgcgttat accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat
      481 actggcctgg attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga
      541 gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt
      601 ggcgttgggc tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg
      661 cgctcattgc catacgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt
      721 tggtggcgca gttgggccga ttaatgcgcc gacggtattt aactcagtat ttaacgttga
      781 gcagttctgg gatggtcgtg cggcaacatt gcaggatcag gcaggtggac cgccgttgaa
      841 cccgattgaa atggcgtcga aatcctggga cgaaattatt gctaagctgg aaaaagatcc
      901 gcagcttaaa gcgcagttcc tcgaagttta tccgcaaggt ttcagtggcg aaaatattac
      961 tgatgccatt gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg
     1021 gttgcgtgga gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa
     1081 agataataaa tgtgcaactt gtcatggtgg tattattctc ggcggacgtt cctttgaacc
     1141 gttggggctg aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat
     1201 gaatgtgact aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt
     1261 tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa
     1321 actgatgctg cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt
     1381 agctttcctg cacagtctga acggggtgta cacgccgtat atgcaggata aacaataatt
     1441 aatttgatcg cccgaacagg aatgtttggg cgatttttat tacgataat
//
LOCUS       DAZOYF010000381         1486 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZOYF010000381 DAZOYF010000000
VERSION     DAZOYF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.391"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUOF_004490"
                     /pseudo
     CDS             complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUOF_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
     gene            complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUOF_004491"
     CDS             complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUOF_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR0613352.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          428 a          326 c          299 g          433 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtgtttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaa
//
LOCUS       DAZOYF010000382         1467 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZOYF010000382 DAZOYF010000000
VERSION     DAZOYF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.392"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            87..491
                     /gene="stpA"
                     /locus_tag="ACHUOF_004492"
     CDS             87..491
                     /gene="stpA"
                     /locus_tag="ACHUOF_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR0613353.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(538..1062)
                     /gene="ygaP"
                     /locus_tag="ACHUOF_004493"
     CDS             complement(538..1062)
                     /gene="ygaP"
                     /locus_tag="ACHUOF_004493"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR0613354.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1072..1371)
                     /locus_tag="ACHUOF_004494"
     CDS             complement(1072..1371)
                     /locus_tag="ACHUOF_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR0613355.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          401 a          339 c          345 g          382 t
ORIGIN      
        1 taaattttgt ggaaatataa taagtgatcg cttacactac gcgacgaaat acttttttgt
       61 tttggcgtta aaaggttttc tttattatgt ccgtaatgtt acaaagttta aataacattc
      121 gcaccctccg tgcgatggct cgcgaattct ccattgacgt tcttgaagaa atgctcgaaa
      181 aattcagggt tgtcactaaa gaaagacgtg aagaagaaga acagcagcag cgtgaactgg
      241 ctgagcgtca ggaaaaaatt agcacctggc tggagctgat gaaagctgac ggaattaacc
      301 cggaagagtt attgggtaat agctctgctg ctgcaccacg tgctggtaaa aaacgccagc
      361 cgcgtccggc gaaatataaa ttcactgatg ttaacggtga aactaaaacc tggaccggtc
      421 agggccgtac accgaagcca attgctcagg cgctggcaga aggtaaatct ctcgacgatt
      481 tcctgatcta attactgagg ccggatgtcg ctgctagggc gcgtccggcg tcgcttctca
      541 agctcgttgg ttccacggca tcttatctaa caaccttgcc attccacaaa aaccgctgat
      601 tcctgcaaac agtaacccgg caccaacaaa gccacttaat aagaagaaac cgctatttac
      661 ggtataaccc agtacaacgc cgtttaatat taaaccaccc gcagcgatct gcacctggcg
      721 cattaacggc aagggttgag atttatttac cgctactggc aatccagctc ttttccagcc
      781 atcaatccca tcttcgagta aaaatatttc tgcgggggcg gcaatcgctg ctaatttatc
      841 ggcgttatta ctggtgcgtt tacctgcctg gcagtggaat ataatttgct cgcgacgtaa
      901 tttaggtgga agacctgact gttccagcac ggataatgga gccagatctg cttcaggaat
      961 atgttcacga agatattcat cagcgtcacg aatatcgatt aactttgcgc cgcgtgcgat
     1021 taattcttgt gcatcatgcg gcgaaatggt tgtcaaagcc atagtgactc cttacggaca
     1081 atagacattt ttcagggtgg cgataatggc atttaccgcc tcatttttaa tggaatatag
     1141 aatgcgttgg gcatcccgtt ggctgttgat aagcccttcg tcccgcatac gagcgagatg
     1201 ctgtgatgtc gcagaggcgc tcagtccggt aatgcgcgtc agctctcccg cgctggtgcc
     1261 gggggaaccg ctaagcatgc acagaatcag caaccgttta gggtggctca ttgctttcaa
     1321 taaggccgct gcctgttcgg tactggcctg taattgcgcg agttcagtca tatttattta
     1381 agtattttct aaattaagta aactctaaac taaaaatgca acatatacca gcctcagcag
     1441 cgtaaatgag agtaaaagcg taagctg
//
LOCUS       DAZOYF010000383         1431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZOYF010000383 DAZOYF010000000
VERSION     DAZOYF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.393"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            428..597
                     /locus_tag="ACHUOF_004495"
                     /pseudo
     CDS             428..597
                     /locus_tag="ACHUOF_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            743..>1431
                     /locus_tag="ACHUOF_004496"
     CDS             743..>1431
                     /locus_tag="ACHUOF_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0613356.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          347 a          330 c          444 g          310 t
ORIGIN      
        1 caacacaaag gcaactaata aagatacaga taaagatcta aaaacagatc taacccatcc
       61 caaacccttc ccttccggaa gggagtttcg ggattttgtg gctggagtgc ttgaggggag
      121 attatctggc ggtactgcag cggaattttg taattctgcg gtggttgcgt tgcaggctgc
      181 tggcctggat gtctgtcgtg agtatccggt gccagagcgt ggtgacggtt gcggagggcg
      241 gattgatatc gtcgtgactg acaggaacgg tgtccggtgt gggatcgagc ttgaccgaaa
      301 ttctccgcga cagaaatcac tgctcaaaat cggtgctgtt gaaaccggga tatgtgtctt
      361 gcggcgcagt gatatcgcaa ggcacaccga gcagggaatt ctggttatcg gtggggctgt
      421 tcgccagaaa aaatttgacc cgttgtcagt tgatctgccc gactggttgt cagaaacact
      481 ctggcatgag tgggtccagt tcaggcaggc attgcgaaaa ccgattcgaa cggagctggg
      541 cgctaacggg gcgatacggg aactggaaaa attccgtcag cagggtttta cacctgagca
      601 ggtgattcga cacagcatcg ccaatgaata ccagggtctg ttcgcgccga aaggtgttcg
      661 gcctgagacg ttgctccgac aggttaacac cgtctcgttg ccggacagtg cgatcccgcc
      721 aggcttcagg gggtaacaga ccatgaaaaa tattgcgaca ggaggcgttc tggagcgtat
      781 ccgcagactg accccaccac atgtaaccgc cccattcaga acggttgcgg agtggcgcga
      841 gtggcaactt gctgaaggcc agaaacgttg cgaggagatc aaccgcctga atcgtcagtt
      901 gcgggtggaa aaaatcctga accgctccgg catccagccg ttgcaccgga agtgttcgtt
      961 tgcgaattac cgggcgcaga acgacggtca gcgacatgca ctgagtcagg cgaaatccat
     1021 agctgacgaa ctgatgacag gctgtacgaa ttttgtgttc agcggtaagc ccggcaccgg
     1081 aaaaaatcat cttgcagccg cgattggcaa ccggctgatg gcgaaggggc gtagcgtgat
     1141 tatcgtcacc gtgtccgatg tcatgagcgt gttgcatgag agctacgaca acggcaaatc
     1201 cggtgaaaaa tttttacagg agctttgtgg tgttgacctg ctggtcctgg atgaaattgg
     1261 catgcagcgg gatacgaaaa acgagcaggt ggtactgaac cagattgttg atcgccggac
     1321 ggcatcgtta cgcggtgtgg ggatgctgac aaatattaac catgcagcga tgaatacact
     1381 tctcggcgag cgggtgatgg atcgcatggt catgaacggc gggcgctggg t
//
LOCUS       DAZOYF010000384         1423 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZOYF010000384 DAZOYF010000000
VERSION     DAZOYF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.394"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(1..507)
                     /locus_tag="ACHUOF_004497"
                     /pseudo
     CDS             complement(1..507)
                     /locus_tag="ACHUOF_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(529..1069)
                     /gene="yhaB"
                     /locus_tag="ACHUOF_004498"
                     /pseudo
     CDS             complement(529..1069)
                     /gene="yhaB"
                     /locus_tag="ACHUOF_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
     gene            complement(1325..>1423)
                     /locus_tag="ACHUOF_004499"
     CDS             complement(1325..>1423)
                     /locus_tag="ACHUOF_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFR0613357.1"
                     /translation="ISNTGIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          442 a          253 c          264 g          464 t
ORIGIN      
        1 tgtgaaatca taataatcta aatcgacatt acgtaagtcc agagaggata gatcaactat
       61 atctccagtc ccacaactaa agattacgtt caacctcctg atttcattgt aaatatcttt
      121 taatgggcaa ttaacattga tattgcctgt agatatttcc tagtttttta acggttcatt
      181 aatggtaagt tcgcaggtat tgtccgatcg ttgaataatt tgggtgttat aacctaaaaa
      241 atccagacga agagacgagt cattataaag aattacattt tgtaagtacg ctaattcttt
      301 ttgaaaatca ccagtgataa tgcattttcc acttggttgt aacgcgttaa gtaaagtgac
      361 catttgaata gaggtctgtt ttagtttttc tatacctggt gctagtgctt cgaggttatt
      421 tcctatgggg ctttcaggaa ggtcattcat ttttcgacgg actaaatcac tactaatatc
      481 attaccaatt gatgaaacag ggaacattat tttatccttt ataaataatc aataaaaatc
      541 attattaaaa tgatcgggtg aaaaaaagag aatttgccaa caatctgaag taatgcaggt
      601 tttattaaag tgcctagggg gtgttaagat ggcttctttt tgggtgaact ttgcgcaata
      661 cttagaaatc gtctccttaa tacattccag ctctttttta ttgacggctg agcaagtaca
      721 ttgttcacca aaaggtgcaa aagtgcctac atctaatgtt tgtatgatat aatcatcatg
      781 ttctcctgta gaaatatcta tgatgaatgg actcatattt tccggttgga ttcggaatca
      841 tgacagaagg aacatcgcct tgatagaaat tcaatgcagt aattgcagat aatactctcc
      901 ggagttcatt acttatactg taaattttat taacgtcatt ggtggagaca atttctgcaa
      961 actttctttt taccgtccaa taatcaatat ttctattgtg attgttaacc actgcgtgca
     1021 ttggcgggat tgaagggtga aaaatatgtg caattgggaa gcctttcatt gatgcctgcc
     1081 ttgtcgtcat gacaaacaat aatagatgct tattttcctc gcacgacact tacgcactat
     1141 caaaaaacgc tcatttaccc ggcgttacag taaacgaagc aacattctgt gtcctacgct
     1201 gatgtaaagc acagtgaaaa tcccccttgt tgacttcgtc tgaccttatc gcggcgacca
     1261 gcaattattc gttttgtgat cctcaacaca aaatgctatt cgtcgcctat gaggggagaa
     1321 gaatttatgg aaaacgattg cattcacaaa atgacgtcct gtcagaagag aaaaagttga
     1381 cgttaatcaa ttgttcgaga tgatcaatac ctgtattcga aat
//
LOCUS       DAZOYF010000385         1414 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZOYF010000385 DAZOYF010000000
VERSION     DAZOYF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.395"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..294
                     /locus_tag="ACHUOF_004500"
     CDS             <1..294
                     /locus_tag="ACHUOF_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR0613358.1"
                     /translation="SAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLFE
                     SLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            300..1397
                     /locus_tag="ACHUOF_004501"
     CDS             300..1397
                     /locus_tag="ACHUOF_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0613359.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          438 a          320 c          347 g          309 t
ORIGIN      
        1 agcgcttatc cgatttttga tcgtattgaa gaaatggcat gggctcgtca ctaccagcag
       61 atcgttcgtg aagaaaaaga aacggaactg gcggacgacc tggaaaaagg tctgcctcag
      121 cacctgtttg aatcgctctg catcgaccat ttgcaacgcc acggtgccaa taaacaggca
      181 atcagccacg catttgatga tgatgttgag tttcaggagc gcatggcaga acacatccgg
      241 tacatggctg aaaccattgc ccaccaccag gttgatattg atttggagga ctaaaatcta
      301 tggcgctttt tcaacgagca ataaacacac aggcctatct aaaagcagga attatggggt
      361 ttgctggcga cggcaaaacc tacacagcaa gcgaactggc tattggtctg gtaatgctta
      421 tgcggcaacg gggaattgaa gctggtaacc gcccggtaat gtttctggac acagaaacag
      481 gctctgactg ggttaaaccc cgctttgatg cggaaaatat cgaattattt acggcaaaaa
      541 cccgctcgtt tgttgatctg cttgaagcca ttaatgaagc ggaaagcagc ggttccgtaa
      601 tgatcatcga ctccatcagt cacttctgga ccggattatg cgacgagtat gccagacgcc
      661 gaaaccgtaa acgaggactg gaattttcag actgggcgtg gctcaaacag gagtggcgac
      721 gctttaccga tcgatttgtt aacagtcagg cgcacattat tatgtgcggg cgtgcaggat
      781 atgagtatga ctttttcgaa ggtgatgatg gcaaacgcca gttggcaaaa accggtataa
      841 aaatgaaagc agaaacagaa accggttatg aaccctccat ccggatccag atggagaagc
      901 aaatgaacat agaaaccggt caggtgtggc gcaccgccag aatactaaaa gatcgctcca
      961 cccgtattga tggacaggta ttttcaaacc caacatttaa aaactttctt cctcatatcg
     1021 agtctcttaa cctgggaggt gaacaccccg gcatcgatac ctcacgggat aacagtgaat
     1081 tatttgccaa cgacggtaca ccgacatggc taaaagaaaa gcgtgcaaaa gaaatcgcac
     1141 ttgatgaaat tatcgagttg ctcaacaagc accatggggg caccagtaac gacgccaaac
     1201 gcgcaaaagc tgatttgctg gaaaaaacat tcacctcccg ctcatgggaa agaatcaaag
     1261 gtatggactg gccgacaatc aaagctggtc gtaatgcact atggattgaa ctggaaggcg
     1321 ttgaatatgc attcccggat ccacaaacac aaaacgaaac gcaacaagca ggatacgatg
     1381 agaatattcc ggtgtaaaac caagtgcatt ttgg
//
LOCUS       DAZOYF010000386         1404 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZOYF010000386 DAZOYF010000000
VERSION     DAZOYF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.396"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..167
                     /locus_tag="ACHUOF_004502"
     CDS             <1..167
                     /locus_tag="ACHUOF_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFR0613360.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="ACHUOF_004503"
     CDS             152..790
                     /locus_tag="ACHUOF_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFR0613361.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="ACHUOF_004504"
     CDS             1018..>1404
                     /locus_tag="ACHUOF_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613362.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          393 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DAZOYF010000387         1342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZOYF010000387 DAZOYF010000000
VERSION     DAZOYF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.399"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            124..1342
                     /locus_tag="ACHUOF_004505"
                     /pseudo
     CDS             124..1342
                     /locus_tag="ACHUOF_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          494 a          265 c          181 g          402 t
ORIGIN      
        1 gcctaaaaaa atgatatttc tgatagttat aaactgaaat ttcttaatct tgattataac
       61 aagctagcat cgctggatgt ctcccggcaa gaatctctga ttgagttaag tgcccatcac
      121 aatatgatca ctgaccttat attacacaat catcccagga tgaaaacaat cactttaaac
      181 gacaaccata ttgcacattt aaacgccaaa aacactacaa aactggaata tttaaactta
      241 agcaataaca atttactgcc aaccaatgac attgatcaac taatatcatc aaaacatctt
      301 tggcatgtat tagttaacgg catcaacaat gatccacttg cacaaatgca gtactggact
      361 gcagtaagaa atataattga tgacactaat gaagtgacca ttgatttatc aggacttaat
      421 ttaaccactc aaccaccagg gctgcaaaac ttcacctcta tcaatcttga taataaccaa
      481 ctcacacatt ttgatgcaac caactacgat agactcgtaa agctaagtct gaatagtaat
      541 gctcttgagt caataaattt tcctcaaggc agaaatgtaa gtattacaca tatatctatg
      601 aataataatg ctctcagaaa tattgatata gataggcttt catcagttac ttattttagt
      661 gcggcacata atcaactaga gtttgtgcaa ttagaatctt gcgaatggct gcaatacctg
      721 aatctcagcc ataatcaatt aactgatatt gttgcaggta ataaaaatga actcttactg
      781 ctggatctat cccataataa actaacaagt ttacacaatg tcttatttcc caacttgaat
      841 acgttactta ttaacaacaa tttgctttct gaaattaaaa tattctatag caacttccgc
      901 aatgttcaga cattaaacgc tgctaacaac cagttgaaat atataaatct tgatttcctg
      961 acttatcttc catctatcaa aagtttaaga ctggacaata ataaaataac ccacattgat
     1021 actaataata catccgacat tggaacttta ttccccataa taaaacagag caaaaactta
     1081 aattttttaa atgtttctgg gaagaacaat tgacctacta tgcagctcat gttatttaat
     1141 ttgttttccc cagcacttaa gcttaatact ggcctggcaa ttctttcgcc tggtgcattt
     1201 gaagttcact ctgacggaat agatgcggat aacgaattgt ttcactatac tattaaaaaa
     1261 gcatataccc catataatat acacacttac aagacagagg aagttgtaaa ccagaggaat
     1321 ataaaagtta aaaacatgac ct
//
LOCUS       DAZOYF010000388         1337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZOYF010000388 DAZOYF010000000
VERSION     DAZOYF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.400"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(208..363)
                     /gene="hokD"
                     /locus_tag="ACHUOF_004506"
     CDS             complement(208..363)
                     /gene="hokD"
                     /locus_tag="ACHUOF_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0613363.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(591..1256)
                     /locus_tag="ACHUOF_004507"
     CDS             complement(591..1256)
                     /locus_tag="ACHUOF_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR0613364.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          331 a          342 c          314 g          350 t
ORIGIN      
        1 tccatccctc ttctccctgc gcgctactta agtgagtcga ttctatctgc gaaggtgcgc
       61 gaatcaaatt caccggaaat aaacaacaaa aaacccgccg aaacgctgag agatcacagc
      121 gatgggcggg ttaagtgcgg gtgcgttgag gatgcctgac acatcagagg tgggcgggga
      181 tgggatcagc tccccacccg gtcactctta cttcctggat tcgtagtcta cgaagacagc
      241 gacctccgtc tggccggttc ggattcgtac ctcacagagg tctttcctcg ttaccagtgc
      301 cgtcactatg acggttaaac agatgacgat cagggcgatt aacatcgcct tttgctgctt
      361 catagcctgc ttctccttgc ctttcggcat gtaagaggct aacctacatg tgcaaagcat
      421 gaaattggcc tcagattaat gttaagcgtc ttgccggacg cgtaatgtta actggggctt
      481 ttctctatct gccttttggt gttcatgcct gaagcagata gcctcaagca cccgcagtca
      541 ttctacttac ctcgccaata tgaaatcaat cagaaaggtg ccccataaaa tcactccttc
      601 tcttctttac cgtagtggag ttgaccaatt ttgataagag ggcgtccctg agatttgcgg
      661 tgtagattgg tatcgcgcag agaatacaca cagccacaat attcctgctg atagaatttt
      721 tcgcgcttgc tgatttcaat catacgggac gagccgccct gcttgcgcca gttataatcc
      781 cagtacacca tgcccggata atgcgcgacg gctcgccgcc cacagtcgtt aacctgctgc
      841 atatttttcc agcgtgaaat gcccagtgaa ctgctgatca cactgaaacc attttcagcg
      901 gcgtacagcg ctgtccgctc aaaacgcatg tcaaaacaca tggtgcaacg gatccctcgt
      961 tcgggctccc attccattcc tttggctcgt tcaaaccagt tgtctgtgtc gtaatcagca
     1021 tcaataaacg gcacgccgtg ttgttcagca aagcgaatat tctcatcctt acgaattaaa
     1081 tactctttct gaggatgaat gttcgggttg tagaaaaaga tggtgtagtc gatttccgag
     1141 gcctgaagcg cctccatcac ctcaccggaa catggagcac agcaagagtg cagtagtagt
     1201 ttgtttgccc cgtttgggag ctccaatttg ggccgtttga aatcagcaac tgtcataaat
     1261 atgtttattg aggtcataaa aatatcaaag agtgtagcat tagaacgggg ctatcggaaa
     1321 cagatgtgtg actgctc
//
LOCUS       DAZOYF010000389         1330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZOYF010000389 DAZOYF010000000
VERSION     DAZOYF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.401"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(41..100)
                     /locus_tag="ACHUOF_004508"
     CDS             complement(41..100)
                     /locus_tag="ACHUOF_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0613365.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1265)
                     /locus_tag="ACHUOF_004509"
                     /pseudo
     CDS             complement(339..1265)
                     /locus_tag="ACHUOF_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
BASE COUNT          266 a          354 c          321 g          389 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca
//
LOCUS       DAZOYF010000390         1321 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZOYF010000390 DAZOYF010000000
VERSION     DAZOYF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.402"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            49..1321
                     /locus_tag="ACHUOF_004510"
                     /pseudo
     CDS             49..1321
                     /locus_tag="ACHUOF_004510"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          370 a          239 c          334 g          378 t
ORIGIN      
        1 ttatgccatg catattccat gttttatcga ctgtcattaa ttttgataat ggccttactt
       61 gcgggtcagt taagtgccca ggagtggtca tttgattctt cacagttgga aggaaacgtt
      121 agtgcggaca ctgtggcaat gtttaatcag ggagagcaac tccccggaaa ttatcgtgtg
      181 gaaatttatc ttaacggtga aaaagttgat gttggggagt ttccctttca tcgtccggaa
      241 tcccctgaag aaaaagagct tgttccatgt ttaaccgttg atgaccttat ccactatggc
      301 attaaaattg ataaaagcag cagtgatact gacaataaaa agaatcagtg ttttaaatgg
      361 aactctattg aaggtttgaa agtaaattat gattttgata gccagcgcgt acagataacc
      421 gttccacaac tgtatctgca agataaaaaa agtagtttag caccagtgtc gttatggaat
      481 gagggtgtag ctgcattccg catggtgtat cagacaaata ttgatatttc caaacaaaat
      541 gataatcaat ctaccacgcg taattctcgt tatggcaggt tcactccagg gtttaatctt
      601 ggtgcctgga ggtttcgtag ttcagtgaca tggagcaagg aacttggcca gtcagaaaga
      661 tggcaaaggg gttacatgtg gtttgagcga ggcataaatg cgattaaaag ccgattaacg
      721 cttggagaat cttatacgtc gtctgaggtg tttgacagca taccttttcg cggcgggatg
      781 ttagcgaccg acgatgcgat gactccgccg gaggacagct actacacacc tgtggtgcat
      841 ggaatagcac aaagtgaggc gcaggttatt attaaacaaa atgggcaaat tatttttacc
      901 cgttctgtgc cgccagggcc ttttgcgctg gataatttac caacgctggc cgttggcggg
      961 gagcttgatg ttacagtgag agagagtaat ggcgaggagc agtatttttc cgtgccattt
     1021 caaacgccgg ccatcgctct gcatgaagga tatttcaaat atagtgtgat gggggggaat
     1081 ataaagaaga aggtataaaa agctcccctg tctccgtggg cgaaatcacg gcgatgtacg
     1141 ggcttcctta tggcgtcacg gtgtatggag ggattcaaaa cgccactcat tttaatgcaa
     1201 tatctacggg tattggcatt tctctggggc tgttagggag tttatcgaca gatattacgc
     1261 gttcgattgc gaatttatat tatgggaata aatatcgtat tcgttatagt aaatccatta
     1321 g
//
LOCUS       DAZOYF010000391         1287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZOYF010000391 DAZOYF010000000
VERSION     DAZOYF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.403"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..368
                     /gene="spa40"
                     /locus_tag="ACHUOF_004511"
                     /gene_synonym="spaS"
     CDS             <1..368
                     /gene="spa40"
                     /locus_tag="ACHUOF_004511"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HFR0613366.1"
                     /translation="QEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGI
                     YFNPEIAPAPFISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFE
                     HLDEVLRLIVWLEQVENTH"
     gene            390..623
                     /locus_tag="ACHUOF_004512"
     CDS             390..623
                     /locus_tag="ACHUOF_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator YmgB/AriR"
                     /protein_id="HFR0613367.1"
                     /translation="MGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKDIIN
                     VLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT          420 a          202 c          247 g          418 t
ORIGIN      
        1 agcaagaggg acactttgag acaaagtcga gaaggcgtga gttgcatatc gagattcttt
       61 cagagcagac taaatctgat atacgtaatt caaaattagt ggtaatgaac ccgactcata
      121 ttgcaattgg tatttatttt aatccagaaa tagcgcctgc accttttatt tctctcattg
      181 aaactaacca gtgtgccttg gctgtcagaa aatatgcaaa tgaagttggt ataccgactg
      241 tgcgtgatgt gaaattagct agaaaactat ataaaacaca tacaaaatat agttttgttg
      301 attttgaaca cttggatgaa gtcctacgtc ttattgtttg gcttgagcag gttgaaaaca
      361 ctcattaata agtaaaggag atgtgttata tgggagttaa tttctgtaat aaaataggta
      421 ttgatcagtc tgaatttgaa atagaatctt ccatcattaa ctccattgct aatgaggtat
      481 tgaacccaat atctttcctt agcaataagg atataataaa tgttttactc agaaagattt
      541 catctgaatg tgacctggtt agaaaagaca tttatcgttg tgctctggag ttggtcgttg
      601 aaaaaacacc tgatgattta taatcattca taataactct tttgctgata taaatatttt
      661 tgttgaagat atttatgtag tcacaggatc aattgtagaa aattgttatg tttttgttag
      721 gaactgcact aaataatata tgataagaca gcaaaaacga ctgaccataa tccttctttt
      781 attaggagtt gataagagag actattcatc ctgtaatgtt aaaactctgt tatatagtat
      841 tcgcgattat gcaaagtctg taaatgatca tgagatacta accgagagta atcgtttatt
      901 atctcattgc atttcagata gtaacggagc tttctttaaa agtagtaaat acgttcccct
      961 taagtactta cgtaaaagga gaatagcacg aaagatacca aatgattagc actttgctag
     1021 taagcttgct ggtaaactat attgaccgtt gggtttgttt tgagactgta atttaaattg
     1081 tgtaattgcc tgtttttgat atgtttactc caacaacgga gacaggtaaa ttatggacga
     1141 aaagaagctt aaagcccttg cggctgaact ggctaaaggc ctcaaaactg aggccgacct
     1201 caatcagttt tcccgtatgc tgacgaaata tcccggctct taaaacgata gtggctcagg
     1261 ctttcatttt tcagcgttcc ccagaag
//
LOCUS       DAZOYF010000392         1284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZOYF010000392 DAZOYF010000000
VERSION     DAZOYF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.404"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            522..806
                     /locus_tag="ACHUOF_004513"
     CDS             522..806
                     /locus_tag="ACHUOF_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFR0613368.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            806..1081
                     /locus_tag="ACHUOF_004514"
     CDS             806..1081
                     /locus_tag="ACHUOF_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0613369.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          339 a          321 c          324 g          300 t
ORIGIN      
        1 acagaataag tttacgcgta aatcgccctg aatccagaag agcattaagt atcacataaa
       61 caggttccat accagtcctg accgcatcag aacgccagta aataaaatct cgtagtccac
      121 cggcatcaga gacaagagta atcgtcgtta ccggtatttc gactggtttg tactggtgtg
      181 agcggacaaa actactgtac gtctctcata cacgctcgta agcggccctc acggcccgct
      241 aacgcggaga tacgccccga ctgcgggtaa acccttgtcg ggaccactcc gaccgcgcac
      301 agaggcattt tcatcgctga caggccgcat ggcctcgcag agccggaggg ggactggtga
      361 tattcggacg accgtaccgg cttgcgccgg gcttcggcgg ttttacacct gtgctacatg
      421 taaccaccgt atgccgcctt atatgcgctg actcgcatta tttgccttgt tccgtctgaa
      481 agtaatcact atgattaaat atgattaaca actaatcggg tatgcaaatg aaaaacaata
      541 ccgcacaagc aacaaaagta attaccgcgc atgtgccatt acctatggct gataaagtcg
      601 accagatggc cgccagactg gaacgctccc ggggctgggt tatcaaacag gcgctttctg
      661 catggcttgc ccaagaggag gagcgtaatc gcctgacgct ggaagccctg gacgatgtga
      721 catccggaca ggttatcgac catcaggctg tacagtcctg ggcggacagc ctcagtactg
      781 accatctgtt accggtgcca cgctgatgga actgaagtgg accagtaagg cactttctga
      841 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgctgccg ccagaacggt
      901 gcagtccctg acacaggcgc cggtcattct gttaactcat ccacgtatgg gagaacagtt
      961 gtttcagttt gaacccaggg aggtcagacg gatttttact ggcgagtacg aaatccgtta
     1021 cgaacttact ggccagacta tttatgtatt gcgcctgtgg cacacacgag agaacaggta
     1081 gattgggtgt ggaggcaact ttaacatagc ctgcaggtat taattctgga gcattgttat
     1141 caggccagat attttgcatt ccattaaata atcttgtagg agataaaata aattacgatg
     1201 aaataacgaa aattaccgct cgtgactggc ggcaatatcg cgctccgggc tggcaaataa
     1261 cccatcagaa acgttattgc caga
//
LOCUS       DAZOYF010000393         1277 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZOYF010000393 DAZOYF010000000
VERSION     DAZOYF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.405"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="ACHUOF_004515"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="ACHUOF_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFR0613370.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          359 a          229 c          285 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actatctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DAZOYF010000394         1266 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZOYF010000394 DAZOYF010000000
VERSION     DAZOYF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.406"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..644
                     /gene="spa29"
                     /locus_tag="ACHUOF_004516"
                     /gene_synonym="spaR"
     CDS             <1..644
                     /gene="spa29"
                     /locus_tag="ACHUOF_004516"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFR0613371.1"
                     /translation="RIPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSF
                     CLSLPFWIFHAVGSIIDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMV
                     FILESIQLSYNICPLFSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVL
                     SRFAPQMNAFSVSLTIKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            651..>1266
                     /gene="spa40"
                     /locus_tag="ACHUOF_004517"
                     /gene_synonym="spaS"
     CDS             651..>1266
                     /gene="spa40"
                     /locus_tag="ACHUOF_004517"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HFR0613372.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYM"
BASE COUNT          346 a          188 c          205 g          527 t
ORIGIN      
        1 ttagaatacc tgtgattttt cttgttgcat caggattaat tacttctggt aaggtagaca
       61 taggttcttc tgtttttgaa catgtttatt tccttatgtt caaggaaata attgttggcc
      121 tccttctctc tttttgcttg tctcttccct tttggatatt tcatgctgtt ggtagcatta
      181 ttgacaacca gcgtggggca acgcttagta gttcaattga tcctgccaat ggtgttgata
      241 cgtctgagtt ggcaaaattt ttcaatcttt tttctgcagt tgtatttcta tacagtggtg
      301 gtatggtctt tattttagaa tccatacaat tgtcttataa tatatgcccg ttattttctc
      361 aatgttcttt ccgtgtctca aatatcttaa catttctgac tttattggca agtcaggctg
      421 ttattttagc cagtcctgtt atgatagtat tgttactatc agaagtatta cttggtgtat
      481 tatcgagatt tgctccgcag atgaatgctt tttccgtatc attaactatt aaaagtttac
      541 ttgcaatatt tattattttc atctgttctt ctactattta cttttctaaa gttcaatttt
      601 tcctcggtga acataaattt ttcacaaatc tatttgttag ataaaatatt atggcaaata
      661 aaacagaaaa gccgacacct aaaaaactaa aggatgccgc aaaaaaagga cagtcattta
      721 aatttaagga tttaacgact gttgttatta ttctggtagg gacatttact ataatatcat
      781 tcttttcctt aagtgatgta atgctcttat acagatatgt aataattaat gacttcgaaa
      841 ttaatgaggg taaatacttt tttgcagtgg ttattgtctt ttttaagata attggcttcc
      901 cacttttttt ctgtgttctt tcggctgtgt tgccaacatt ggttcaaaca aagtttgttc
      961 ttgcgactaa agctatcaag attgattttt cagtattaaa ccctgttaaa gggttaaaaa
     1021 aaatatttag tataaagaca ataaaagaat ttttcaaaag cattctgctt cttattattc
     1081 tagcattaac aacctatttc ttttggatta atgaccgaaa aataattttt tctcaggtgt
     1141 tttctagtgt tgatggctta tatcttattt gggggaggct gtttaaggat ataatattat
     1201 ttttcttggc attttctatt cttgttatta ttcttgactt tgtgattgag ttcattttat
     1261 acatga
//
LOCUS       DAZOYF010000395         1254 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZOYF010000395 DAZOYF010000000
VERSION     DAZOYF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.407"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(1..570)
                     /locus_tag="ACHUOF_004518"
                     /pseudo
     CDS             complement(1..570)
                     /locus_tag="ACHUOF_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            578..1004
                     /gene="tnpA"
                     /locus_tag="ACHUOF_004519"
                     /pseudo
     CDS             578..1004
                     /gene="tnpA"
                     /locus_tag="ACHUOF_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          327 a          314 c          283 g          330 t
ORIGIN      
        1 ggcaagttta gccacgccag catccagccc gaccattgat gctgaagggt gaaccggagt
       61 tgatacttca ctttctgcct gaatactgat gtaccactta ccgcaggact ggctgacagt
      121 gacatttttc acaacacccg tgacctgacg gctgttccgg tagcgcatcc agccaagttt
      181 tggcagaaaa atacggctgt tttcctgagc gagcttaacg ccctgcggat agcggaatgc
      241 atcattctgt caccgctttt tgaatcaggg gaacgccgag cgcttctgga aaaagttttt
      301 gcaggcctgc tcagggtctt tcagtgactg ttgcaatggc tgtgacggag catctttaat
      361 ccattgcgtt tcagtggcat ttttccactc aaccaatcag gaagccattt tcgcgtaagg
      421 gatgtctttg ttaccggcct tatgattctc attctgacgt gccagagtac gactgaaaac
      481 gaaacgacat gcgcctgcga agcgcctcat ttcacgcgcc tgttgaccac cgggtcttaa
      541 ccggaatttg aatacctgta gtcgtttcac agggcaaatt atactttggt ctgtgaaaaa
      601 aactgatatc cgacgcggaa ggcactgtgt tttcctgatg catgctcacc gggtctttgt
      661 taccaagtac cgacgccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt
      721 ttcaaatgta tgtgcttatt ttgaagctga actggttgaa atggatggcg aaccagatca
      781 cgtccatctg ttaattaact atccccccaa actggcgata tccagtctgg taaacaaaca
      841 gcctcaaagg cgtatcgggt aggttactgc gacgggatcg accagatatt gctgccaggt
      901 attaccgcaa agatgttttg tggcgtcccg gctattttgc cagtagctgc ggaggtgcgc
      961 caatatccgt catccgccaa tacattgaac aacagcaaac acctggtcag atgaaaaaac
     1021 gagccttata tccccgccct gaaggacggg cttttacggt gcactggata agcctggaga
     1081 ttatctacac ctgcatggac atcattgtac tatgccccgt acatgctcca tcacatcctc
     1141 aacatcaaag tattagagcc ttgcaaacca ttatgcgtct cagtttgtct catctatctt
     1201 acgcaaaaaa aatagaacta atatcaagcg gcgctatatt gcgtattaaa taaa
//
LOCUS       DAZOYF010000396         1244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZOYF010000396 DAZOYF010000000
VERSION     DAZOYF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.408"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..763)
                     /gene="ospC2"
                     /locus_tag="ACHUOF_004520"
     CDS             complement(<1..763)
                     /gene="ospC2"
                     /locus_tag="ACHUOF_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFR0613373.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFK"
     gene            1167..>1244
                     /locus_tag="ACHUOF_004521"
     CDS             1167..>1244
                     /locus_tag="ACHUOF_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0613374.1"
                     /translation="MSIRCPSCSATEGVVRNGKSTAGHQR"
BASE COUNT          392 a          192 c          232 g          428 t
ORIGIN      
        1 ctttaaagtt atctaagaaa aatgattggt gctcatggta aaaaacaggt ggaatagcat
       61 tatcaaagtg gtcggttaat gtcatatatc catatggaga gtcatgatca atgatatacg
      121 cattagcacc aaaatccaca gtatgatgtg ttttatttag tgggagtgtt tcttgatgat
      181 tggaaatttc aaccccaaaa aaaacaaagt cgtggttagc tatttcttca atatcctcag
      241 agaatgtgtt tttatatgtt tgtatattct cagacttaat tttatcaaag gacttaatat
      301 taagagtatt atttgacagg ttagcgttac tttgatgtct gaagttaaaa tcagaattaa
      361 taataataga gagcaagtga cgttcatccg aagaaagagg atgagttttt tgtattattt
      421 cagaaagata gtgtctggac accttatcga ggtgctcaag tgaccgaaat gatgaccgtg
      481 ctccagatgg cgcttcaata gaaagattta atgatttgaa actggtccct tgagagaaca
      541 ttttactata actttgcgct gctatagact tccttaaaaa gcttttcaca gtatcagcac
      601 agtgatctaa atgtttttca cttatgccag aagaggatga gtttgttacg catgatatgt
      661 ttttttgtaa tgcttttgta tctttgttcg gatttatttt tccatcaaca agatcaatat
      721 tgttctgaac attaatatga tttactgctt caggtatttt cataaaacat cccccattat
      781 gtgcgttttt atctgcagtt atttagaata atattatcta acttaatatt ttcttgtatg
      841 tattataacg aacatatttt aatatgttgt acgattttca ctctgaaatc ccctgagttt
      901 aatcacactt cctttactga aaggaattat tgcaacagta ttatttgttt ttggatatat
      961 atcttgcgcc ttgttatgta gttaatgctc agaatatgat agataaaata gagacgcaaa
     1021 acagggcatg ctcataacat gatgataaca taaaaaatta gtaacaataa atgaaagaat
     1081 catgggcgct caacaaaaag agggtaatgc tgccaactta ctgatttagt gtatgatggt
     1141 gattttaagg tgcttgcgta gcttccattt ccatcagatg tccttcctgc tccgctactg
     1201 aaggcgtggt gcgtaacggc aaaagcactg ccggacatca gcgc
//
LOCUS       DAZOYF010000397         1243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZOYF010000397 DAZOYF010000000
VERSION     DAZOYF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.409"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHUOF_004522"
     CDS             complement(<1..143)
                     /locus_tag="ACHUOF_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFR0613375.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
     gene            complement(545..>1243)
                     /gene="ydeO"
                     /locus_tag="ACHUOF_004523"
     CDS             complement(545..>1243)
                     /gene="ydeO"
                     /locus_tag="ACHUOF_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR0613376.1"
                     /translation="DYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKKYLQCT
                     NIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAACKG
                     FITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQI
                     LLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRH
                     TGMNTGNTMSALAI"
BASE COUNT          386 a          235 c          237 g          385 t
ORIGIN      
        1 gtaaagtctg agaaagcaac gtttgttttg gttgctttca gctttacatc atctgccgca
       61 tgagcagcaa atgcagccat accagatgcc agtatcaaag atatcgaggt acttacgaca
      121 cttttcttta atgcagactt cataaatgtt cctcttctta ttgtacttaa ttccgaagta
      181 attttacgaa gagaaataag tggatgtaag tgaagttagt cacataaaga gatagcagat
      241 ttagctaaaa aaagggaaat aacagtccat aaagcgttga cattactttc tgttctatta
      301 agtaatttct cgccgataaa caactaattt attgatattt aataaattat tgcattttac
      361 tgacaaaatg ccgaattgag atcataaata atcatgcaac aggttatgca agtgcataaa
      421 tatgtgatgg atgtcactta tttatttcaa taaatatatc gcctaaaaac aacgcggggc
      481 agggaatggc tgccccatat aattcttacg tagcgtgcgt ggttgactac tcgttagcaa
      541 ataatcaaat agctaaagca ctcatcgtgt tgcccgtatt catacccgtg tgcctttgac
      601 aacggtactc cttagaaact ctcttcggac tgttgccgaa atgtttgcgg aacgcataaa
      661 taaaataaga tgtactggca taaccacatt gttcggcaat tttattgact gaaccttcta
      721 cgcgtatcaa attttttgcg tgctgcattc tggcatctaa tagaatctgt gagaatgtcg
      781 tttgctcttg ctttagtttt ttcttcaaca ggctttcact gatgtacagg cagtcacaaa
      841 tatctttcag cttccatggg tgcgccagct tcatgttgac aatatttctc actttcccgg
      901 aaacggatag cacaccgtta gttaatagtg taatgaaacc tttgcatgcg gcaaaaatag
      961 acaggcaaga aaaaagcaaa agttctgaaa aattatgatg attgcgttct tccgagttga
     1021 gataagcaat catttcatta agcaatccag tcggtacatt tgaagcacgt aaaaacttcg
     1081 caggaacagg caccgtcact gtctggatgt tagtgcattg tagatatttt ttaatggtat
     1141 tttcattaaa tccgatttct ttaaaatgcc gctcataagg ttcaaaatgt gtacgaacag
     1201 cgttatctac catcaggatc tcgccgtcgg agaaggcgta atc
//
LOCUS       DAZOYF010000398         1243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZOYF010000398 DAZOYF010000000
VERSION     DAZOYF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.410"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            91..1089
                     /gene="yiaK"
                     /locus_tag="ACHUOF_004524"
     CDS             91..1089
                     /gene="yiaK"
                     /locus_tag="ACHUOF_004524"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFR0613377.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1101..>1243
                     /locus_tag="ACHUOF_004525"
     CDS             1101..>1243
                     /locus_tag="ACHUOF_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFR0613378.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT          310 a          327 c          319 g          287 t
ORIGIN      
        1 ttgttaaaaa gtgatcgata tatttgaaat ctagttccgc atattgaaat tttaagccaa
       61 aaaagcgatc aaaaaacaag gaagcctagg atgaaagtga catttgagca gttaaaagca
      121 gcctttaatc gggtcttaat ttcacgcggc gttgacagcg aaacggctga cgcctgtgca
      181 gagatgttcg cccgcaccac cgaatccggc gtttattctc acggcgttaa tcgtttccct
      241 cgtttcattc aacaactgga aaacggcgat atcattcctg atgcccaacc caaacgtata
      301 accagcctcg gcgcaattga acagtgggat gcccagcgtt cgatcggcaa cctgacggcg
      361 aaaaagatga tggatcgcgc cattgaactg gctgccgatc acggtattgg tctggtggca
      421 cttcgtaatg ccaaccactg gatgcgcggc ggcagctacg gctggcaggc ggcggaaaaa
      481 ggctatattg gcatttgctg gaccaactcc atcgccgtaa tgccgccgtg gggcgcaaaa
      541 gagtgtcgca tcggcactaa cccgctgatc gtcgccattc cttccacgcc gatcaccatg
      601 gtcgatatgt cgatgtcgat gttctcttac ggcatgttag aagttaaccg cctggcaggc
      661 cgtcagctcc cggtcgatgg tggctttgat gatgagggca atttgaccaa agaacctggc
      721 gttatcgaga agaatcgccg cattttgccg atgggctact ggaaaggttc tggcatgtcg
      781 attgtgctgg atatgatcgc tactctcctt tccgacggcg catcggttgc cgaagtcacc
      841 caggacaaca gcgacgaata cggcatttca caaattttta ttgccattga agtggacaag
      901 cttatcgacg gtcccacccg cgatgccaag ctgcaacgta tcatggatta cgttactacc
      961 gctgaacgcg ctgacgaaaa ccaggccatc cgcttacccg gccacgaatt tactaccctg
     1021 ctggccgaaa accgccgtaa cggcatcact gttgatgaca gcgtgtgggc caaaatccag
     1081 gcgttatgag gagataagtc atgatttttg gacatatcgc gcagcccaat ccgtgccgtt
     1141 tgcccgccgc cattgaaaag gcgctcgatt ttctgcgcgc taccaacttc aacgtcctgg
     1201 agccgggcgt tgtcgaaatc gacggcaaga atatttatgc gca
//
LOCUS       DAZOYF010000399         1222 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZOYF010000399 DAZOYF010000000
VERSION     DAZOYF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.411"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..82)
                     /locus_tag="ACHUOF_004526"
     CDS             complement(<1..82)
                     /locus_tag="ACHUOF_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFR0613379.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLI"
     gene            complement(148..387)
                     /locus_tag="ACHUOF_004527"
     CDS             complement(148..387)
                     /locus_tag="ACHUOF_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HFR0613380.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(522..1127)
                     /locus_tag="ACHUOF_004528"
     CDS             complement(522..1127)
                     /locus_tag="ACHUOF_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0613381.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          359 a          274 c          208 g          381 t
ORIGIN      
        1 aaataagaaa gagcgcaaca aataaaagat cgactcttag taaccatatt gctgccattg
       61 aaagactcca tgtaccatac aaggttgctg ccagacatat tcctttacgt ctgacagcct
      121 ctagagagtt tttttatttt ttataagtta cggattactc gttccggaaa tcgttacctg
      181 ttgaattgct gcagcgatag cgtcgaatgc atctttaatt gcgcccagga agcctgagtt
      241 ctgatcgcca aagatgaaag caagcagagt tgccatagca acaccaatca atgcatattc
      301 gattgcagta acaccacgtt catccttcgc gaaacgcgca aagcactgtt tagttttacc
      361 atagtactta gtaataaaag tattcatgtt ttcctcgtat attattattt aaattcaaac
      421 caccagcttc atctgcgtgt caccggtatc ccatattttt gggatgtggc tataattatc
      481 tattcccgct atttagtctt caaaaaaaat tacatttaat atcatttatc ttgattaaga
      541 aatagtaaga ctaaaaaaac aagcgtcgtc agccagcaca ctgctaacac ggtaagaacg
      601 ctaataaaaa cactccactg cttcgttcta taccattcaa tatttttgtc agtatgtttt
      661 attgggagca gctcttcagt agagaattcc tggctgttat cattctcagg aatgtttgca
      721 tcagtgttta cttccctggc ctcgctatcg ttggtatagc tactactgtc ttcatcactg
      781 tcacgctcat tctcacagac atgtttctcg acctttccca gatatctgac gccttttcgc
      841 ggaatggttt taattagaga actcgacaaa cctgcgacgt ctaatgcctt tctaagcatg
      901 taaatctgcc cataataact gctgtcgctt accgaacctt tctgctcccg ccaaacctca
      961 ttgataatat gttgcttatc ggattctccc tgtagtaaaa ggagtaaaaa tcgatagccg
     1021 ttttcagaca gattgattac attgcctgta ctggtggtga tacgtcgaga cgctgggtaa
     1081 aacttaactc cctgccctaa agaaaaataa aaacggtcat cgctcataag taccgacccc
     1141 tgttttcttc ttcacatgac tactcattac aactttgtat gaagtaagat gcaaaatcat
     1201 attgtttaca ttaagttaag ca
//
LOCUS       DAZOYF010000400         1217 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZOYF010000400 DAZOYF010000000
VERSION     DAZOYF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.412"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(108..479)
                     /locus_tag="ACHUOF_004529"
     CDS             complement(108..479)
                     /locus_tag="ACHUOF_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613382.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(540..1037)
                     /locus_tag="ACHUOF_004530"
     CDS             complement(540..1037)
                     /locus_tag="ACHUOF_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613383.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(1066..1217)
                     /locus_tag="ACHUOF_004531"
                     /pseudo
     CDS             complement(1066..1217)
                     /locus_tag="ACHUOF_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase"
BASE COUNT          286 a          316 c          290 g          325 t
ORIGIN      
        1 cataccttgg cctaggtaaa gaagatcaga ctcgtagcca catcggtggc aactgagctt
       61 gtacgcaatg cccatctggt tgtctcacct gaatcgtaac gtcagagcta ttcatccatc
      121 cattcggcat caatgtatgc cactaagccg accagctgac cgtttggact gaagtatgtt
      181 gcctgcaccc cacttgacat ctcatcaata ccaccgcact gcgctaggcg ccggtcccac
      241 ttgcccacag aagcgtcagg atcggctgag ttccccaaga cccttgtgag ccatgcctcc
      301 tttaactcaa cctctcgaat gattgcgtcg gccagctcct ccgcgttatc tagttcgaca
      361 tagtctggaa ttcggacgta cgttctgaac acagctggat acttacttgg gcgattgtca
      421 tacatgacat caacaatgta cccgtttgtg taaccgtctc ttggaggttc gtatgacact
      481 agtggttccc ctcagcttgc gactagatgt tgaggcctaa cattttatta gagagcaggc
      541 tagttgctta gatacatgat cttcaggccg ttatctgtca gggcaagcga aaattggcca
      601 tttatgacga ccaatgcccc gcagaagctc ccatctttgc cgccatagac gccgcgcccc
      661 ccttttgggg tgtagaacat ccttttgcca gatgtggaaa agaagttcgt tgtcccattg
      721 ttggcaatga cgtagtagcc ggcgaaagtg cgagacccat ttgcgctata tataagccta
      781 cgatttccgt tgcgactatt gtcgtaattg gatgaactat tatcgtagtt gctctcagag
      841 ttgtcgtaat ttgatggact attgtcgtaa ttgcttatgg agttgtcgta gttgcttgga
      901 gaaatgtcgt agttggatgg ggagtagtca tagggaagac gagcttcatc cactaaaaca
      961 attggcaggt cagcaagtgc ctgccccgat gccatcgcaa gtacgaggct tagaaccacc
     1021 ttcaacagat cgcgcatagt cttccccagc tctctaacgc ttgagttaag ccgcgccgcg
     1081 aagcggcgtc ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg
     1141 cctttcacgt agtgaacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct
     1201 tgtccaagat aagcctg
//
LOCUS       DAZOYF010000401         1211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZOYF010000401 DAZOYF010000000
VERSION     DAZOYF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.413"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..353)
                     /locus_tag="ACHUOF_004532"
     CDS             complement(<1..353)
                     /locus_tag="ACHUOF_004532"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR0613384.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVF"
     gene            complement(383..>1211)
                     /gene="wzx"
                     /locus_tag="ACHUOF_004533"
     CDS             complement(383..>1211)
                     /gene="wzx"
                     /locus_tag="ACHUOF_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HFR0613385.1"
                     /translation="GDEKFGIVNIQKSISSSCIAGIPAIFVFYSATLSAAVAGLIFAR
                     VISILVSAYYVRNDIKISGVHFCYKTFKRLFFFGGWMTVSNIISPVMVYFDRFIVSNI
                     MGADKVAFYSAPAEVILKLGIIPAAIGRAVFPRLSNIKDFKEFKRNVNKSLLLMFLIC
                     LPVIIIGLLYSGLVLKIWFGENYQINSFNILNVLLIGFFFNALAMIPFSAIQALGKSK
                     ITALIHCAELVPYLALLYFMVEKYGLLGAAISWSIRVILDALLLQWLYTRMCSVYEN"
BASE COUNT          428 a          239 c          167 g          377 t
ORIGIN      
        1 ataaaaactg ggccagctat ttttgcattt tcattatcca tatagcagga aagttttttg
       61 ataaactcat tgcgaatacg gctatcttga tcaaaaaaaa ttatcttctc tgcccctttt
      121 tcaagagaat atcgaatccc aatattttgt gcttttgcta taccttcatt cctaccaaga
      181 caaattaaat gaactttttc aatgtctgct aatttatctt tcaaggtgaa attagtccta
      241 ttatcgacta ctacaacgta acaattttgc tcctttagtt cagtgatcag tgcattcaga
      301 tttttaagtt cgggattata tgtcacaatg acagcgtaaa tattactatt cattatccaa
      361 gtgactcagt aattggttaa ttttagtttt catatacaga acacattcta gtataaagcc
      421 attgtaataa cagagcatct aaaattacac gtatgctcca ggatattgcg gcacccagta
      481 acccatattt ttcgaccata aaatacaaaa gggctaaata aggaaccaat tcagcacaat
      541 gaatcaaagc agtaatttta gattttccta atgcctggat tgcagagaat ggtatcattg
      601 ccagcgcatt gaagaaaaaa ccgatcaata acacatttaa tatattaaag gaattaattt
      661 gataattctc accaaaccat attttcaata caagccctga atataacaag cctatgatta
      721 tcaccggcaa acagattaga aacattaaaa gcaatgattt atttacatta cgtttaaatt
      781 ctttaaagtc tttgatgtta cttaaccttg gaaacactgc cctcccgatt gctgcaggta
      841 ttattcctaa ttttagaata acctccgctg gtgcagaata aaatgcaact ttatctgccc
      901 ccataatatt tgacactata aatcgatcaa aatacaccat gaccggactt atgatattac
      961 ttactgtcat ccaaccgcca aagaaaaaga gtcgtttaaa ggttttataa caaaaatgaa
     1021 cccccgaaat tttaatatca ttcctgacat aataggcaga gactaaaatc gaaataactc
     1081 ttgcaaatat taaaccagca accgctgccg acaatgtagc actataaaaa acaaatatgg
     1141 ccggaattcc cgcgatgcaa gagcttgata tagatttttg aatatttaca atgccaaatt
     1201 tttcatcccc c
//
LOCUS       DAZOYF010000402         1192 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZOYF010000402 DAZOYF010000000
VERSION     DAZOYF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.414"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..266
                     /locus_tag="ACHUOF_004534"
     CDS             <1..266
                     /locus_tag="ACHUOF_004534"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR0613386.1"
                     /translation="NYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPPYN
                     EQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            487..828
                     /gene="symE"
                     /locus_tag="ACHUOF_004535"
     CDS             487..828
                     /gene="symE"
                     /locus_tag="ACHUOF_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFR0613387.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(875..>1192)
                     /locus_tag="ACHUOF_004536"
     CDS             complement(875..>1192)
                     /locus_tag="ACHUOF_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HFR0613388.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT          351 a          292 c          224 g          325 t
ORIGIN      
        1 caaattactt attgaaatat atcctttcca ttccatttct tatgcaagtc aaaaaagcag
       61 agaacagaat aaaaatgcca aaacttaatt ctgacagttt ttacaatatt attgttgcga
      121 ttccccccta taatgaacaa caagcaatat ttgataagat aaattctatt gaagcagtat
      181 gcaatggtct tatttcttat attgggattt atcataaaac ccaactgcat cttgcagacg
      241 cactcactga cgcagcgata aactaattcc ctcgccctca caaaaccaat aaagcggcta
      301 aatagccgct ttattcacat caccaaaatt tatatttcac gctaattttc atcttatttt
      361 cattaatttt tagctggact ttccctgtat ttactgatga tgtatccagg tatttagcac
      421 ggtgcggatg tgcgccaaca cactcgcacc gctaatcaca atcactattt atagagaata
      481 gtaatcatga ctgacacgca ttctattgca caaccgttag aagcagaagt ctccccggca
      541 aataatcgtc aattaaccgt gagttatgcg agtcgttacc cggattacag ccgtattccc
      601 gccatcaccc tgaaaggtca gtggctggaa gccgccggtt ttgccaccgg cacggcggta
      661 gatgtcaaag tgatggaagg ctgtattgtc ctcaccgccc aacccgccgc cgccgaagag
      721 agcgaactga tgcagtcact acgcaaggtg tgtaagctgt cggcgcgtaa acaaaggcag
      781 gtacaggaat ttattggggt aattacaggt aaacagaaag tcgcctgata cattgacccg
      841 gtagcgccag tgctaccggg tattattcgc cgcgttatac caacgcgctc tccatctgtt
      901 tttcaaatgc ctgacgaatt aatcccataa tgtaaggaag ttgtgcgagg tcgctgaaac
      961 caatttctac atcgccatta ccccagcgcc caacatttgt cacatctttg gcaatacctt
     1021 tcggatcgac cagttcatga aactgcatat taagcgacaa tcgcagtcgg cttttttgcg
     1081 gcacaacatc aacaaaattc gtttccgctt taaacgcaat atatagcttc aatacttcct
     1141 gcgtgatccc tgcgtccagg cgcataactt catcgcgaag atgatcgaat aa
//
LOCUS       DAZOYF010000403         1186 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZOYF010000403 DAZOYF010000000
VERSION     DAZOYF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.415"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1103)
                     /locus_tag="ACHUOF_004537"
     CDS             complement(<1..1103)
                     /locus_tag="ACHUOF_004537"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR0613389.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
BASE COUNT          285 a          307 c          309 g          285 t
ORIGIN      
        1 gtacagatga actcatacgg cccctgtttg gcgatttcat ccgccagata agcggcaacc
       61 tggtaagagg cgttctgtac tttggtatag ccttcacgac cgaggcgcag gaattcatag
      121 tactgtgcaa ttacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatttga
      181 ccacccaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccaggaag
      361 ccaccgctgg cagcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tccagcgcat cgtgcagcgg ttgtgggaac tcatagttac cggtgtaggt cacgccgaaa
      481 gtcggcacca cgccgatggt gttttcgtca caggcttcaa tcatgcgttt cgggtccata
      541 aacaactgac cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat
      601 ttatgccagc agatttgtac cggaccgcac actaggtttg gtttatccgt tggtttgcct
      661 gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc
      721 tcggaagaac caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc
      781 cacagatcgg caaccatatt tacgcaacgc aggtcgatgg ctgcggattg cggatattct
      841 tctttgtcga tccagttttt attgatcgac aaatccatca atttatggac gttttcgtcg
      901 tcccaggtct ggcagaaagt ggctaggttc tgacgagcgt tgccatcaag atataattca
      961 tcattgataa tctggaatgc gacatcatcg cgcatttcgt gcagcggaaa tcgttttgac
     1021 tccgcgatag tagaaatggc ctttgcgcca aaacgtgaat cgagtagttc tgagcggaaa
     1081 tccgttaaca gcttctggtc catttcgaac tccttaaatt tatttgaagg caataaaaaa
     1141 gtaggattta tccgcaatgg aagcaaggca ttacaggctt aattta
//
LOCUS       DAZOYF010000404         1185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZOYF010000404 DAZOYF010000000
VERSION     DAZOYF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.416"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..1103)
                     /locus_tag="ACHUOF_004538"
     CDS             complement(<1..1103)
                     /locus_tag="ACHUOF_004538"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR0613390.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICT"
BASE COUNT          287 a          309 c          307 g          282 t
ORIGIN      
        1 gtacagatga actcatacgg ccccagtttg gcgatctcat ccgccagata ggcagcgacc
       61 tggtaggaag cattctgtac tttggtatag ccttcacgac caaggcgcag gaattcatag
      121 tactgtgcaa tcacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatctga
      181 ccaccgaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccagaaag
      361 ccaccgctgg cggcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tccagtgcat cgtgcagcgg ttgcgggaac tcatagttac cggtgtaggt cacgccgaaa
      481 gtcggcacca cgccgatggt attttcgtcg caggcttcaa tcatgcgttt cgggtccata
      541 aacaactgac cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat
      601 ttatgccagc agatttgcac cggaccgcac accaggtttg gtttatccgt tggtttgcct
      661 gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc
      721 tcggaagaac caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc
      781 cacagatcgg caaccatgtt tacgcaacgc aggtcgatgg ctgcggattg cggatattct
      841 tctttgtcga tccagttttt gttaatggat aaatccatca acttgtggac gttttcgtcg
      901 tcccaggtct ggcagaaagt ggccaggttc tgacgagcgt tgccatcaag atataattca
      961 tcattgataa tctgaaatgc gacatcgtcg cgcatttcgt gcagcggaaa acgttttgat
     1021 tctgcgatag tggaaataga cttcgcacca aaacgtgaat cgagtagttc cgaccttaaa
     1081 tccgttactt gcttcttatc cattttaaac tccttaaaat gattggatcg cattaaaaaa
     1141 gtaggattta tcgataaagt aagcaagtgc aaaggactcg tgttt
//
LOCUS       DAZOYF010000405         1168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZOYF010000405 DAZOYF010000000
VERSION     DAZOYF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.417"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            7..756
                     /gene="phoC"
                     /locus_tag="ACHUOF_004539"
     CDS             7..756
                     /gene="phoC"
                     /locus_tag="ACHUOF_004539"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase PhoC"
                     /protein_id="HFR0613391.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGELAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(811..915)
                     /locus_tag="ACHUOF_004540"
                     /pseudo
     CDS             complement(811..915)
                     /locus_tag="ACHUOF_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(926..>1168)
                     /locus_tag="ACHUOF_004541"
     CDS             complement(926..>1168)
                     /locus_tag="ACHUOF_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0613392.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          308 a          264 c          256 g          340 t
ORIGIN      
        1 tgttatatga aaagacagct ttttactctt agtattgtcg gggtattttc tttaaatacc
       61 tttgcatcat tccctccggg aaatgatgtg acaacaaaac ctgaccttta ctacctgaca
      121 aacgataatg ctattgacag tctggcatta ttaccgcctc caccacaaat cggaagtatt
      181 gccttcctga atgaccaggc catgtatgaa aaggggcgtt tattgcgaaa tactgaaaga
      241 ggggagctgg cagctgagga tgccaatctc agtagtggtg gcgtggcaaa tgtattttct
      301 gctgcttttg gttctccgat aacggctaaa gattcaccag aattgcataa attactgaca
      361 aatatgattg aggatgccgg agacctggca acgcgttcag caaaagaata ttatatgcgt
      421 attcgacctt ttgcttttta tggtgtttct acgtgtaata caaaagaaca ggatacatta
      481 tccagaaatg gctcttatcc atcaggtcat acatcgattg gttgggcaac agcacttgta
      541 ttgtccgaaa taaatccggc acgccaggat accattctga aacggggtta tgaactgggg
      601 gacagcaggg ttatttgcgg ttatcactgg caaagtgatg ttgacgcagc acgtattgtt
      661 ggctctgcta ttgtagcaac actgcactca aatcctgtgt tccaggcaca attacaaaaa
      721 gcgaaagatg aattcgctaa caatcagaaa aaataatgct tttagccagt acgccttata
      781 accccgacct tcagggctaa tgccagtcag ttaagcaact gactggctct ttttcagggc
      841 tgtggggtat ttccagtgcc tctcctttac cactctcgga aaggcccttt cccttcttgt
      901 cggtaatttc actaggctgt gtcccttaat tacgtaagcg ttgataaaag agtcggatgc
      961 agcccagttt cactatcgcc aggtaatttc tcgccgtttt gtcgtaacgc gtggcgatgc
     1021 ggcgatattc tttcagccgc ccaaagcacc gttcaacgac attacgattg cagtacgcat
     1081 cacgatcaag ctgtgcacgt ccatccgatg ccattttctc atttgacttt cgggggatta
     1141 ctgcctttat accgttattt ttcagctc
//
LOCUS       DAZOYF010000406         1160 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZOYF010000406 DAZOYF010000000
VERSION     DAZOYF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.418"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(12..705)
                     /gene="ospZ"
                     /locus_tag="ACHUOF_004542"
                     /pseudo
     CDS             complement(12..705)
                     /gene="ospZ"
                     /locus_tag="ACHUOF_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            761..913
                     /locus_tag="ACHUOF_004543"
                     /pseudo
     CDS             761..913
                     /locus_tag="ACHUOF_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          344 a          224 c          197 g          395 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aatctctggc gactcatctc ttaacacttg
      181 cataatctct ctttttatat catatagttc tataaaccga tttgcccgta gttctccttg
      241 ggaagaatca tttctgaata caacaattat atacaaaaaa tcactatttt ctttagagaa
      301 gatcttacaa ctaatctctg ggagctcaca taatgcatgt aagtcaggat ggtacaaact
      361 atccacagaa aattgcccca taggacgttt tccagatata actgtcaacc agcttccttc
      421 cggtgtactg gcttcggctc tggaaatagg ctgaaaatct tccggacatg gagaatacat
      481 tgtcttcata taattaagtc gtctggattt ctcattaata aactcctcta tttttaattg
      541 tgccatactt ctggttatac cagagatata cttcttgtca tatatgtttg gtgattcatt
      601 aaagtaacca tgttcaactc taggataaat atttctcact atatattcag tatttgcagt
      661 atttattgga aatacgttct taatattctt gatgggacta atcattttaa tctctatact
      721 caaatataaa cattaccatg aacaatataa ttgttcctaa gtggaatgtc tccacggaga
      781 acactttatc tatcgggaaa tagtgcgggc aacagtgttt aattatatcg agtgcaatta
      841 caatcgctgg cgacggcacc gttggtgtgg cggcctcagt ccggaacaat ttgaaaacca
      901 gaacctcgct taggcctgtg tccatattac gtgggtaggc tcacagagcc tcacttacga
      961 ctgcgtccat attatgtggg taggatcaat aaagacgttt tatatgtgtc tgcttgcagc
     1021 cagaaaattt ataccctgca ggtatcataa acatgcttga taacttaatt ggggctccct
     1081 cttcctttta tggtgtaatt gtttgattga atacttccgg ggatttcagt atgaaatgaa
     1141 gtatatttaa tatactttac
//
LOCUS       DAZOYF010000407         1159 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZOYF010000407 DAZOYF010000000
VERSION     DAZOYF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.419"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            115..>1159
                     /gene="cadB"
                     /locus_tag="ACHUOF_004544"
     CDS             115..>1159
                     /gene="cadB"
                     /locus_tag="ACHUOF_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR0613393.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctg
//
LOCUS       DAZOYF010000408         1151 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZOYF010000408 DAZOYF010000000
VERSION     DAZOYF010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.420"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            106..>1151
                     /locus_tag="ACHUOF_004545"
     CDS             106..>1151
                     /locus_tag="ACHUOF_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0613394.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          359 a          270 c          184 g          338 t
ORIGIN      
        1 gcatgatata acactcattc ccttatggcg aatattctga aacaccctcc tcattaggag
       61 agatacatta aatacaattt attcacaatc taattccata tccatatgcg agaaattaat
      121 atgctcagaa acatttcatc ctgtttattt ccacatatca gcacaattac atcccccaac
      181 cattatttgt ccgaatggga tgattgggag aaacaggggt taccggaaga acagcgtact
      241 gaggcggtaa gaagacttcg tgcatgtctt acctctaagg ggcataaact ggacctgcga
      301 gccttggcgc tttcctcgct acctgtactc cctgcttgca ttaaaaagct tgatgtgagc
      361 tgtaataaat taaccatcct tactgattta cctgaaaata ttaaagaact tattgcaaga
      421 gataatttct taacacatat atctgcatta ccacattatc taataacttt ggatgtgtcc
      481 gaaaatcaat tagagaatct gccgttatta ccagacacca tcaaatcact aagcgcagag
      541 tataataggt tatccacact gccttcatta cccttgaatt taaaaaaact tgaggttagg
      601 aacaacgaac tgcaaactct tccatctctg ccttctaatc ttaagatact taaggttgcg
      661 cacaaccatc ttactgaact gcccccttta cctaggagac tgcaacttct ttttgcatat
      721 agcaatagat taagcaactt accaaacatc caagaaaata ttatcatgag aagatttttt
      781 tattttgaaa acaaccaaat aactacaatc ccgacaaatc tttttcgttt agatcctcat
      841 ataactattg agattgcaaa taacccctta tcagatcaaa ctctgctatt cttaatacag
      901 caaacttcgg ttccaaattt taacgggcct cagtttcgta tttccctgtc agaccaaaac
      961 agactgtttt tacgccagat gttgccgcaa aatttacatt cgcgccatat cagagtcatc
     1021 actgaagggg ggcagaactt tcagatcccc cctcttcccg aaactgtggc agcctggttt
     1081 cctgaagcag atcgtcggga ggtttctaca caatggactt ctttttccac cgaggagaat
     1141 tcccgggcat t
//
LOCUS       DAZOYF010000409         1140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZOYF010000409 DAZOYF010000000
VERSION     DAZOYF010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.421"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..730
                     /locus_tag="ACHUOF_004546"
     CDS             <1..730
                     /locus_tag="ACHUOF_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFR0613395.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="ACHUOF_004547"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="ACHUOF_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          298 a          283 c          247 g          312 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
//
LOCUS       DAZOYF010000410         1110 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZOYF010000410 DAZOYF010000000
VERSION     DAZOYF010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.422"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..1019
                     /locus_tag="ACHUOF_004548"
                     /pseudo
     CDS             1..1019
                     /locus_tag="ACHUOF_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..>1110
                     /locus_tag="ACHUOF_004549"
     CDS             992..>1110
                     /locus_tag="ACHUOF_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613396.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          312 a          271 c          259 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag
//
LOCUS       DAZOYF010000411         1096 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZOYF010000411 DAZOYF010000000
VERSION     DAZOYF010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.424"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            41..220
                     /locus_tag="ACHUOF_004550"
                     /pseudo
     CDS             41..220
                     /locus_tag="ACHUOF_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(237..927)
                     /locus_tag="ACHUOF_004551"
                     /pseudo
     CDS             complement(237..927)
                     /locus_tag="ACHUOF_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1006..1096
                     /locus_tag="ACHUOF_004552"
                     /pseudo
     CDS             1006..1096
                     /locus_tag="ACHUOF_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          294 a          248 c          283 g          271 t
ORIGIN      
        1 tgacaaatta aaattactgt aaaaaaacga tacacattta atacgaggga ggccggcaga
       61 aaaacggctt gcggtcaggc aggagaaatg tgtgccgcga atgaagtcct tgtatgactg
      121 gataaagctc cagaggaaaa ctttgtcaaa ccatgctgaa atggtaaagg cgttcaacta
      181 tatcctgaat cactggaatg cactgaatga gttttgccgg gtagtacttc caatcattag
      241 aatatgtgct tttcaatgaa ggaaccgatg actttttcgt ggatttccac ggaacgcgag
      301 aagcagattg ttttacgtgc cagccgcttg atgcgggggc ggagggtcag gttgttacgt
      361 tcatacgttg cgtgaagatt ttgccggtca ggtgtttctc ctccggcact tctctggcgt
      421 agctgcccca gtcatcgctg gtcaccatgc cgatacagaa cggagtcaac agggctaaca
      481 gttcccgaca ggtctcatca ttccgtggcc cgcaagtgta agccaacaca ccgccagttt
      541 tagtgttgta cgcataccag ggccagtgtt gttgcgcctt gctgccaacg aagcgccact
      601 gctcatcaag ttcacagata agggcaacat cagcgtgagc gactggcgca gatgttatcc
      661 gccggggcga gagcttttta atgtccgtat gacggtattg atgccgattt tcagtgtcct
      721 ggcggtatca cggccccctg cgccgttatg tgccatttca acgatctgct ccttaacgcc
      781 gagttttctg gcttcataag agtaagtgag ctggaaaaca cgtttacatg agcgacaacg
      841 gaaacgctca tgccgggaac aactacggcc atgtcgataa acttcatcgg aattacagcg
      901 cggacaatga acagtaacgg tagtcatgga aaacctcaaa taccagtaga ttaccagaaa
      961 cttctatcga ttgaagacat taccttttgc cgcaataggt ggggtaacat tggtgaaaac
     1021 gcattacgcc gggtggcagt tggaaaaaaa ttatcttttt tcggctctga tcgtggtgga
     1081 gaaaatgcgg caataa
//
LOCUS       DAZOYF010000412         1091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZOYF010000412 DAZOYF010000000
VERSION     DAZOYF010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.425"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACHUOF_004553"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACHUOF_004553"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR0613397.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(986..1062)
                     /locus_tag="ACHUOF_004554"
     tRNA            complement(986..1062)
                     /locus_tag="ACHUOF_004554"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Asp,seq:gtc)
BASE COUNT          285 a          255 c          248 g          303 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     1081 caccggtttt a
//
LOCUS       DAZOYF010000413         1085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZOYF010000413 DAZOYF010000000
VERSION     DAZOYF010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.426"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..1038
                     /locus_tag="ACHUOF_004555"
     CDS             <1..1038
                     /locus_tag="ACHUOF_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR0613398.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
BASE COUNT          297 a          264 c          288 g          236 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgc
//
LOCUS       DAZOYF010000414         1070 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZOYF010000414 DAZOYF010000000
VERSION     DAZOYF010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.427"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(228..653)
                     /locus_tag="ACHUOF_004556"
     CDS             complement(228..653)
                     /locus_tag="ACHUOF_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR0613399.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(650..1033)
                     /locus_tag="ACHUOF_004557"
     CDS             complement(650..1033)
                     /locus_tag="ACHUOF_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR0613400.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          253 a          308 c          265 g          244 t
ORIGIN      
        1 tctcgcgtat ggttgattga caccacagtg cgaattcggc gaatgcgaga tcacggtacg
       61 attccgcacc acactaccaa tcaatacccc gggttttatt caggtaagca ggactgcgga
      121 tatccggtgt cgcgcctttg tgtcacgaac ggggtgggtg cgaaacacca gataaaatac
      181 aggttggcaa tacctctgaa cgccctgagc aggtcgggca tttcggatta agtactcgca
      241 cctccgcagt cctcaaacaa gtctggctgg tagctgtaaa cagacttcgt acataccgct
      301 ctgcaataaa tccccgtacc acaggcctca cagaacttct tcccatgaaa aaactgctca
      361 cacatcacac aatgccactc cagcacgacc ggtaatggcg ataaaaacat cgccatatcc
      421 tcaatgtaag ggtgggactt ctccggattc agcaccacac agtccgcctt ctgttgctca
      481 ctcagggcat gtaaatcatg ctcaaaccac gccccctgag catctgtctg cagaatcaac
      541 cggccatggc aggaaaggca gaaacactgc cggatatttc tgctgagcgc agagtcggca
      601 ctgataatgt gctcacccgg cgtgatcccc agccccgttt ttataccgtt cattctgctc
      661 ccccctcctc actgaagcgc ccggtctggc agtgtgtgca gtactgctcc ccataataat
      721 cgtggtgaca ttgcctgcag tgccagctgg ctttacgtac tacgggtaag gtatccggga
      781 cgaattgctg cagacgttta atcaaccgta tttctctgcg ttccggcctg acataagggc
      841 actcatgacc gtgctccgtc agcccttcgt cagcgtgttc aaaccagggc cgttcagtgt
      901 cgtattgcgg gtggtatctg agtgcactgc cgcaaaggtg gcaggtgtag ctatcgtaag
      961 gtgcagtctg tgcggtacgg gcaccggtca gacgtccgtt gccatcaagt gcgagaaaag
     1021 attttgcgta catagtatat gttcctcagc cgccagacga cacgcaggca
//
LOCUS       DAZOYF010000415         1068 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZOYF010000415 DAZOYF010000000
VERSION     DAZOYF010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.428"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHUOF_004558"
     CDS             <1..560
                     /locus_tag="ACHUOF_004558"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFR0613401.1"
                     /translation="TKGDAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDN
                     YQAIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKS
                     GLTGSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMV
                     NEVETALFNARALALKIYSMSGWNL"
     gene            787..>1068
                     /locus_tag="ACHUOF_004559"
     CDS             787..>1068
                     /locus_tag="ACHUOF_004559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HFR0613402.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DAZOYF010000416         1066 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZOYF010000416 DAZOYF010000000
VERSION     DAZOYF010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.429"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(570..749)
                     /locus_tag="ACHUOF_004560"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="ACHUOF_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="ACHUOF_004561"
     CDS             843..>1066
                     /locus_tag="ACHUOF_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0613403.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DAZOYF010000417         1064 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZOYF010000417 DAZOYF010000000
VERSION     DAZOYF010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.430"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(338..1033)
                     /gene="aqpZ"
                     /locus_tag="ACHUOF_004562"
     CDS             complement(338..1033)
                     /gene="aqpZ"
                     /locus_tag="ACHUOF_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFR0613404.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          299 a          322 c          251 g          192 t
ORIGIN      
        1 cctatctgag tgtttagcat tgttgcgctc ctttcatgtg caatgccata taaagccact
       61 ggcggaggga gcgcgcaggg ggcagccagt cgccgccgcc ccctgcaccc ccgggctctg
      121 gcgaacaaaa tcgccgctgc gcggtacctt cagcttatcc ccttcggcaa ccgggtcggg
      181 cgcgaggtaa cgtcctgtaa gacgcgccct cagcccacat ccatgtgggc tgccccggcc
      241 ttcggggaac gcttcagcga ttttgacgcc accaataccc gtgcggtttg taatgtcgaa
      301 gataaaacaa ccagggcaga taaaatgcca atgctaatta atcacgcttt tccagcagag
      361 tccggtaaat cagaccaccg ataatgccgc cgacaattgg caccacccag aagaaccaca
      421 gttgttctaa tgcccagccg ccctggaaga tagcaaccgc ggtgctgcgc gccgggttaa
      481 cagaagtgtt agtcaccgga atactgatta agtgaatcag ggttaaggcc agaccaatag
      541 cgatcggcgc aaaacctgcc ggagcgaatt tgtcggttgc accgtggatc actaacagga
      601 agcccgcact caataccagt tcaactacca gcgcggaaag catggaataa ccgcctggtg
      661 aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc cgcgtcaaaa cccgttttac
      721 cactggcaat taaatacagc agcgccgctg caacaatacc gccgacaacc tgggcaatta
      781 cgtagccaac gacttctttt gccggaaaac gtccgccagc ccataaacca atagtgaccg
      841 ccgggttaaa atgaccacca gaaatatgac caacagcgaa ggccatcgtc agaacggtca
      901 aaccaaatgc cagcgccacg ccggcaaaac caatgcctaa ttccgggaag cctgcggcca
      961 gtacagcact accacagcca ccaaaaacaa gccagaaagt accaaaacat tcagctgcta
     1021 attttctgaa catatccacc tcaattaaaa attgaccctg tgaa
//
LOCUS       DAZOYF010000418         1021 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZOYF010000418 DAZOYF010000000
VERSION     DAZOYF010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.431"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(50..190)
                     /gene="yqcG"
                     /locus_tag="ACHUOF_004563"
     CDS             complement(50..190)
                     /gene="yqcG"
                     /locus_tag="ACHUOF_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFR0613405.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="ACHUOF_004564"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="ACHUOF_004564"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR0613406.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          296 a          205 c          237 g          283 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt tcccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgtta
     1021 t
//
LOCUS       DAZOYF010000419         1020 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZOYF010000419 DAZOYF010000000
VERSION     DAZOYF010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.432"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..81
                     /locus_tag="ACHUOF_004565"
     CDS             <1..81
                     /locus_tag="ACHUOF_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613407.1"
                     /translation="EYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            78..>1020
                     /gene="gtrII"
                     /locus_tag="ACHUOF_004566"
     CDS             78..>1020
                     /gene="gtrII"
                     /locus_tag="ACHUOF_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HFR0613408.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPAGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRL"
BASE COUNT          320 a          183 c          141 g          376 t
ORIGIN      
        1 gaatatatag gtagaatcta tattgaagta aaaaatagac ccaaatacat cataaaaaaa
       61 tctcatcgag gtaacccatg attaaaatta atttatttaa aaatgcaaat ctccttgcct
      121 tcatatcatg ttttgcaata tcaatttatt gttattgggg gtggttgtac gatggaacac
      181 ttaatattga tggcgagttt acaaataatt tttatcaaac aatcacgctt gggcggtggt
      241 ttcacacttt tttgcgacat tactttctcc ctgagccttt ttcactttat ataacaccat
      301 taatagcctt atcttttatc atcatttcag catttataat ctgcagatcg ctaaagctag
      361 aatcttatga attattgata ggtatgttag tatttattac cttccctcag atctcctatc
      421 aattagagtt tcttaaccaa gctgatactg tgggaattgc ttttctactg gcagcgatat
      481 cagcaattat ttttcactcg caaaaaaata ggattgtgat attttctggt atagtactgt
      541 ccattctttc aatggcaatc taccaaacat tcgtaacata tattattgca ttcgtcattg
      601 ggttgcagat aaattcgata atacgaaatg agaaaaatat tcgtgaatct ttttatagtt
      661 catgtttatc tctatccctc atagctttat ctaccttaat ttacctgcta ttaaccaaag
      721 ctatcaagca ttatttttcg cttgaatcga acgagtacat ctcaaattat atacaaaatg
      781 caagcgatat taaatggctt gttaaatcag ccatagataa tatatataac ttctataaca
      841 atcctcccgc tggtttaaac ctatacaagt ggttactgat tcctttatta attctgatgt
      901 ttaccctaac atataaatta aaaacaagat caatttattt gatttcatca atcattttca
      961 tttatatact tccggttata tttatcgttg ttgttggctc aggggcgcca cctcgcctgt
//
LOCUS       DAZOYF010000420         1004 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZOYF010000420 DAZOYF010000000
VERSION     DAZOYF010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.433"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            238..>1004
                     /gene="ipaH7.8"
                     /locus_tag="ACHUOF_004567"
     CDS             238..>1004
                     /gene="ipaH7.8"
                     /locus_tag="ACHUOF_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HFR0613409.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
BASE COUNT          334 a          206 c          143 g          321 t
ORIGIN      
        1 tacttactcg gcttacattt ccttgctcac tggaatgttg aattcagtgt tattattgac
       61 tgcagccaca ataaaaaaag aattccatag aaaacctcca taaataaatt acaactaact
      121 tctgttatgt gtaaaatgga aactattaaa acttaatatc ggaaatggta agtgaaattt
      181 gtataaatat acaattttaa atatttattc tcacaaatat aaggttgacc tagcattatg
      241 ttctctgtaa ataatacaca ctcatcagtt tcttgctccc cctctattaa ctcaaactca
      301 accagtaatg aacattatct gagaatcctg actgaatggg aaaagaactc ttctcccggg
      361 gaagagcgag gcattgcttt taacagactc tcccagtgct ttcagaatca agaagcagta
      421 ttaaatttat cagacctaaa tttgacgtct cttcccgaat taccaaagca tatttctgct
      481 ttgattgtag aaaataataa attaacatca ttgccaaagc tgcctgcatt tcttaaagaa
      541 cttaatgctg ataataacag gctttctgtg ataccagaac ttcctgagtc attaacaact
      601 ttaagtgttc gttctaatca actggaaaac cttcctgttt tgccaaacca tttaacatca
      661 ttatttgttg aaaataacag gctatataac ttaccggctc ttcccgaaaa attgaaattt
      721 ttacatgttt attataacag gctgacaaca ttacccgact taccggataa actggaaatt
      781 ctctgtgctc agcgcaataa tctggttact tttcctcaat tttctgatag aaacaatatc
      841 agacaaaagg aatattattt tcattttaat cagataacca ctcttccgga gagtttttca
      901 caattagatt caagttacag gattaatatt tcagggaatc cattgtcgac tcgcgttctg
      961 caatccctgc aaagattaac ctcttcgccg gactaccacg gccc
//
LOCUS       DAZOYF010000421          990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZOYF010000421 DAZOYF010000000
VERSION     DAZOYF010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.434"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            82..>990
                     /locus_tag="ACHUOF_004568"
     CDS             82..>990
                     /locus_tag="ACHUOF_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HFR0613410.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          301 a          206 c          228 g          255 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt
//
LOCUS       DAZOYF010000422          979 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZOYF010000422 DAZOYF010000000
VERSION     DAZOYF010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.435"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            423..>979
                     /locus_tag="ACHUOF_004569"
     CDS             423..>979
                     /locus_tag="ACHUOF_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0613411.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          352 a          188 c          158 g          281 t
ORIGIN      
        1 gaaaaacaaa gccaacggta ttctgatttc tgaagttaat ttcagactcg tttaagctcc
       61 attttcaaaa aaaatatatc agggtttgtt actaataact tctgaaaaca gaaaaaaata
      121 gagcagtaat tctatttcag agaggagtaa ctccatctat cctcgatacg tactgaacgt
      181 tatcgtgata tattcatcat gctttcttca ccattattcg agtataggga gagacgcaat
      241 gaccttaaat acctttttat ggctgaatta ccattctcag gaataacagc agactccttc
      301 ccatagacgg cttaggggga aagcaatacc cggagaaagg gtacaaagca aactgtttta
      361 aaattaataa gccatattct atttaactag aattcttatc gttgataaga attctagtta
      421 taatgattaa atcaaccaat atacaggcaa tcggttctgg cattatgcat caaataaaca
      481 atgtatactc gttaactcca ttatctttac ctatggaact gactccatct tgtaatgaat
      541 tttatttaaa aacctggagc gaatgggaaa agaacggtac cccaggcgag caacgcaata
      601 tcgccttcaa taggctgaaa atatgtttac aaaatcaaga ggcagaatta aatttatctg
      661 agttagattt aaaaacatta ccagatttac cgcctcagat aacaacactg gaaataagaa
      721 aaaacctatt aacacatctc cctgatttac caccaatgct taaggtaata catgctcaat
      781 ttaatcaact ggaaagctta cctgccttac ccgagacgtt agaagagctt aatgcgggtg
      841 ataacaagat aaaagaatta ccatttcttc ctgaaaatct aactcattta cgggttcata
      901 ataaccgatt gcatattctg ccactattgc caccggaact aaaattactg gtagtttctg
      961 gaaacagatt agacagcat
//
LOCUS       DAZOYF010000423          955 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZOYF010000423 DAZOYF010000000
VERSION     DAZOYF010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.436"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..318
                     /locus_tag="ACHUOF_004570"
     CDS             <1..318
                     /locus_tag="ACHUOF_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613412.1"
                     /translation="FSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGH
                     EMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNV
                     ASI"
     gene            321..866
                     /locus_tag="ACHUOF_004571"
     CDS             321..866
                     /locus_tag="ACHUOF_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0613413.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            890..>955
                     /locus_tag="ACHUOF_004572"
     CDS             890..>955
                     /locus_tag="ACHUOF_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0613414.1"
                     /translation="MSVVISGALTDGAGIPMSGYHI"
BASE COUNT          277 a          208 c          247 g          223 t
ORIGIN      
        1 ttcagccaga gaaacccgga taacagcata gtgttcactg ttaacggaca ggtaattccg
       61 ttaaattacg gaaacttcga tgcccgctat aaatatcgaa cagagggggt acaggatgta
      121 cggtatggcc atgaaatgta ttacagcccc ggcagtaaca ccgtttcgtg gagattttgc
      181 gcaccttcgg gacacgggct gtcagggatg gcgatatcgg ataccggccg taactcagcg
      241 gataacgttg acggtgtgta ttaccgacca ctgcaaaaac tgattaatgg cacctggtat
      301 aacgtagcga gtatttaaca atgttgcatt taaaaaatat tactgcgggt aatccgaaaa
      361 cagcagaaca atatcagatg acaaaacgat attcggtcac ctggcttttt tcagaagacg
      421 gaaaaaactg gtatgaagag ctgaagaatt tcgccagcga cacaataaaa atagcttaca
      481 ccggagatgg tcgcgtggtg tgggtcggta aggatgtgac aggcatcgag ccacgcaatg
      541 ccagcgttat cgaagttccg gatattaccg ctaaccgacg gattaccgcg ccgggttact
      601 ggttttaccg caatgatgaa tttgtctttg actacagact caaagcggaa gatgagcgtg
      661 atgcccttct ggctcaggtc agtgcccgga caggggaatg ggaagaagac ctgctgctgg
      721 ggttaatcag cgacgaagat aaagaaaagc tgaaagcctg tcgtatttac gcgaaatcgc
      781 tgcaggcgat ggatttcagc accatcactg ataaatcctc atacaacgcc attgaatggc
      841 ccgcctctcc ggaaggttct tcctgattta atttatcgcg agaaaaacaa tgtctgtagt
      901 gatatcaggt gcgctgactg atggcgcagg tatccccatg tccggatacc atatt
//
LOCUS       DAZOYF010000424          933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZOYF010000424 DAZOYF010000000
VERSION     DAZOYF010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.437"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(55..243)
                     /locus_tag="ACHUOF_004573"
     CDS             complement(55..243)
                     /locus_tag="ACHUOF_004573"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613415.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            complement(540..778)
                     /locus_tag="ACHUOF_004574"
                     /pseudo
     CDS             complement(540..778)
                     /locus_tag="ACHUOF_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          245 a          181 c          217 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aag
//
LOCUS       DAZOYF010000425          925 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZOYF010000425 DAZOYF010000000
VERSION     DAZOYF010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.438"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(29..625)
                     /gene="fimE"
                     /locus_tag="ACHUOF_004575"
     CDS             complement(29..625)
                     /gene="fimE"
                     /locus_tag="ACHUOF_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFR0613416.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          298 a          202 c          170 g          255 t
ORIGIN      
        1 tttttaactt tattatcaat aagttaaatc aaacctcttc tctttttaat ttttcgttta
       61 tgagattatt tctttcccat aatccggcaa aacgagcagc attactggcg gtataacgca
      121 cagtatggcg aatatttcga tgcccgagat aatcctgaat taaacgggta tctgcaccac
      181 gctccgccag ttcataaccg caagcatgcc ttaacatatg aggatgagtc tgcgttacgg
      241 ttccagcttc aataccggca tcgcgaataa tgcgataggc ctgctggcga gaaagccgac
      301 tcccgcggcg agaaataaat atagcgtcgg tccggtcagc gcctttccag ttagcacgtt
      361 cctgggtcca gcgttccacg gcttcacgct catcaaaacg taacgggtga acggtagaaa
      421 atccgttctt cagtcggcga atatttattc gaccttcatt aaggtcaagg tcctgataat
      481 gcagatcaag cagttcacta atacgcatcc catgccgata tgccaacaga ataagacaat
      541 aatctctggc tcccgttgcc ccgtaacaaa ccgcctgcat catggcctga acttctttac
      601 cggtaagata acgacgttta ctcacaatag tagtactcct aactgagata tatttgaatg
      661 aatacctata ggaaacctca atcggtcaaa attagcccaa tagataaaaa gataaccaca
      721 cacgaagtta tgtggctatc agtcaattac attaaacatc atacaaaaat aaaatatcac
      781 tgatgtgaca acatctattt tcattgattt gattcaatat aacaatagat tacagaggat
      841 taacttccag ttacttaaaa aataaagaaa aaagtgtgga aacaggagaa tttaagtgat
      901 gtgtaaactt atgaatgatt ttgtt
//
LOCUS       DAZOYF010000426          923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZOYF010000426 DAZOYF010000000
VERSION     DAZOYF010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.439"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..200
                     /locus_tag="ACHUOF_004576"
                     /pseudo
     CDS             1..200
                     /locus_tag="ACHUOF_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..>923
                     /locus_tag="ACHUOF_004577"
     CDS             300..>923
                     /locus_tag="ACHUOF_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR0613417.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          293 a          217 c          205 g          208 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
      361 tcgtcctgtg ggcaagcgac gattttgaat cgaccagcac cactctggac tatctgctcg
      421 ttaagtcagg taaaaaactg agcaactatt ttaaagctgt tgccacaaat tttcctgtcg
      481 ttaatgacct tccccctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
      541 gcaaagactc catgacatgg gaactaaaac cgggagcagc gccagacgac gttcaccacc
      601 aggataatgc tcaagaaacc aaagaactgg cgggaggcca ggaagaaaac gcgcaggcag
      661 acgcccacga ggattgccag gattgcgaag tctctgtagc cactttgcgg ttcacacagc
      721 gtcttctgca catttttacg tatgctgccg gggatctgaa atacctgcat catgccaccc
      781 gtgaacaacg caaacacatt actgctcttg agatggatca ggaaaacagc tatgtccaga
      841 atctgctgtt ggccatacgc ggcatggcag aaccgacaac tctggataat gccgccctgc
      901 tccgcctgac tgatgcaatt aag
//
LOCUS       DAZOYF010000427          900 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZOYF010000427 DAZOYF010000000
VERSION     DAZOYF010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.440"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..308
                     /locus_tag="ACHUOF_004578"
                     /pseudo
     CDS             1..308
                     /locus_tag="ACHUOF_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            341..742
                     /locus_tag="ACHUOF_004579"
                     /pseudo
     CDS             341..742
                     /locus_tag="ACHUOF_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          260 a          193 c          246 g          201 t
ORIGIN      
        1 tgctgggcgc tgaaagaact ggcaaaggat atctggagca gaccatggag cgaggaaaga
       61 cgcaacgact ggcagagatg gctgcggcca acagtgacgt ccccatgatg aagaatgtgg
      121 ctaaaacgat agggaaaagg ttgtacggta tcctgaacgc catgcgccat ggcgtatcaa
      181 acggaaatgc ggaagcactg aacagcaaga tcaggctgct gagaataaaa gccaagggct
      241 accgcaaccg ggagcgcttt aagctgggag tgatgttcca ctatgggaaa ctgaatatag
      301 cgttctaagc cttcccacca tgatcgggga agccccagat atgaacaggc acattcagag
      361 ttactttaaa gagcttactg aacgtctgag gtgttagagc catgactgcc gcagcattgc
      421 tggcggaaat gcctgaatcg agcagcctct cgagacgtga aatcagcgca cttgtcggtg
      481 ttgctcaggt aaaccgggac tcgggaactc tgcgcggtcg acgaaccatt ttcggtgatt
      541 gtgctgggga agaacagctc tgtacatggc ggcgcttgag gccccccccc ggtttaatct
      601 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      661 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      721 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      781 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      841 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
//
LOCUS       DAZOYF010000428          880 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZOYF010000428 DAZOYF010000000
VERSION     DAZOYF010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.441"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            103..>880
                     /locus_tag="ACHUOF_004580"
     CDS             103..>880
                     /locus_tag="ACHUOF_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0613418.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITT"
BASE COUNT          303 a          174 c          152 g          251 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcatc
      661 cccccctttc cagataagct tgaagggctg gctctggcta ataattttat agaacaacta
      721 ccggaattac cttttagtat gaacagggct gtgctaatga ataataatct gacaacactt
      781 ccggaaagtg tcctgagatt agctcagaat gccttcgtaa atgttgcagg taatccattg
      841 tctggccata ccatgcgtac actacaacaa ataaccaccg
//
LOCUS       DAZOYF010000429          870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZOYF010000429 DAZOYF010000000
VERSION     DAZOYF010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.442"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>870
                     /locus_tag="ACHUOF_004581"
     CDS             <1..>870
                     /locus_tag="ACHUOF_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR0613419.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DAZOYF010000430          852 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZOYF010000430 DAZOYF010000000
VERSION     DAZOYF010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.443"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..>852)
                     /locus_tag="ACHUOF_004582"
     CDS             complement(<1..>852)
                     /locus_tag="ACHUOF_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR0613420.1"
                     /translation="ARTEATNDLYFSRVSQWDDWLSNYTTLQYRGQFDVVRPEVRKLV
                     AEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMRHNTAKIAVNVGVREQIESGVGAW
                     RLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDANSKQMDKSDAKHCTVINALSRNGW
                     KEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVYVAEYYEVEEEKEKVFIYRDPLTG
                     EPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRVYKSIITCTQILKDREKIAGEHIP
                     IVPVYGEW"
BASE COUNT          179 a          237 c          207 g          229 t
ORIGIN      
        1 ccattcgcca tacactggaa cgattggaat atgctctccg gctatcttct cgcggtcttt
       61 cagtatctgc gtacaggtga tgatcgactt atacacacgc cgacgcttca ccttgcgctc
      121 tgctacctta atgaatccac gattagccag gtcgtcgatg acgtctttga tatcctgctg
      181 gtaatagctg accggctcac ctgtcagcgg gtcgcggtag atgaagacct tctccttctc
      241 ctcttctacc tcgtaatact cagcgacgta gacgacatca ttcgacaccc acgggaatag
      301 ccatgtatcg ttaggattct ggaaagatgg cagggtatcc gggtcaatac cgtaatcctc
      361 tgcgaactct ttccagccat tgcgcgacaa ggcgttaatc accgtgcagt gcttagcgtc
      421 gctcttatcc atctgcttgc tgttggcgtc ccatatgacg tgtgagcagg cttcatggat
      481 tggcaggcgt cggattacct gattgttgct tgttggatcg ttgtcttcgt actgcgtgac
      541 cagacgccat gcaccaacgc ctgactctat ctgctcacga acgccaacgt taacggcaat
      601 ctttgccgtg ttatggcgca tatcagtacg atacatcccc atcaatacat cggctgcatc
      661 aggattagcg ccgtctttgg gtcggaagag aacgtcgaca gggttccggc gcatctctgc
      721 gaccagtttc ctgacctccg ggcgaacaac atcgaattgt ccgcgatatt gcagggtagt
      781 gtagtttgat agccagtcat cccattgcga cactcggcta aaatacaggt catttgtcgc
      841 ctcggttctg gc
//
LOCUS       DAZOYF010000431          836 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZOYF010000431 DAZOYF010000000
VERSION     DAZOYF010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.444"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>836
                     /locus_tag="ACHUOF_004583"
     CDS             <1..>836
                     /locus_tag="ACHUOF_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR0613421.1"
                     /translation="IDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GL"
BASE COUNT          270 a          159 c          184 g          223 t
ORIGIN      
        1 attgattttg attttgttgt cggcgctgac ggtgtgaaga ttttatcacc tatattaatt
       61 tgtgagcgtt tctttttttg gcagttacga tcatttaaat tagatgtaag aggatacgcg
      121 agacatttta aagtattaaa tagctgctta tttgcattac cccccattgc agaacaagag
      181 cgaatagttg aaaaagttag ctctttgatg tccctctgcg accaactaga acagcaatcc
      241 ctgaccagtc tggacgcaca tcagcaactg gttgaaaccc tgttgggaac gcttacagac
      301 agccaaaaca ccgcggaact ggctgaaaac tgggcgcgta ttagcgagca tttcgacacc
      361 ctatttacca ccgaagccag cgtggatgcg ttaaaacaga ccattctgca actggccgta
      421 atgggcaaac ttgtgccgca ggatccgaat gacgaacccg cctctgaact gctcaaacgt
      481 attgcgcagg aaaaagctca attggtgaaa gaagggaaaa taaaaaaaca aaaaccactg
      541 ccgccaatta gcgatgagga aaaaccgttt gaacttccgg aagggtggga atggtgtcgt
      601 attggtaata tagttaatat taaatctgaa ttagtatcac caaaagatta tttaaattta
      661 tatcaagtag cacctgatat tattgaaaag ggcactggga gagtgatatc aaagagaaca
      721 gtgaaagaat caggagtcaa agggccaaat agtcgctttt ataaaggaca aattgtttat
      781 tctaaaatcc gcccgtcatt atccaaagtt tttttagcag agtataatgg tctttg
//
LOCUS       DAZOYF010000432          831 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZOYF010000432 DAZOYF010000000
VERSION     DAZOYF010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.445"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..326
                     /locus_tag="ACHUOF_004584"
     CDS             <1..326
                     /locus_tag="ACHUOF_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR0613422.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="ACHUOF_004585"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="ACHUOF_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR0613423.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          154 g          241 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca agttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgctccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga g
//
LOCUS       DAZOYF010000433          803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZOYF010000433 DAZOYF010000000
VERSION     DAZOYF010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.446"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            46..803
                     /locus_tag="ACHUOF_004586"
                     /pseudo
     CDS             46..803
                     /locus_tag="ACHUOF_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          234 a          169 c          198 g          202 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct
      301 gctgctcact ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa
      361 gagcgcggag aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata
      421 tcagttgatc cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag
      481 gatcttgaaa ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa
      541 gccttagctc atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc
      601 ttgatgagct tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg
      661 ctctcagcaa gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg
      721 agaatagagg ccgatacgga taccgtaggg taacgctgtc tcttcatcga gaagggaaac
      781 agattaacca taaagctgtt cac
//
LOCUS       DAZOYF010000434          791 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZOYF010000434 DAZOYF010000000
VERSION     DAZOYF010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.447"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            40..>791
                     /gene="ipaH3"
                     /locus_tag="ACHUOF_004587"
     CDS             40..>791
                     /gene="ipaH3"
                     /locus_tag="ACHUOF_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR0613424.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          234 a          204 c          131 g          222 t
ORIGIN      
        1 ttcttttaac aaagccattt gtccatcggc tttaactgaa tgtccatcat gttaccgata
       61 aataataact tttcattgtc ccaaaattct ttttataaca ctatttccgg tacatatgct
      121 gattactttt cagcatggga taaatgggaa aaacaagcgc tccccggtga aaatcggaat
      181 gaagcggtct ccctacttaa agaatgtctc atcaatcagt tcagtgagct tcaactgaat
      241 cgtttaaatc tgtcctcgct acctgacaac ttaccacctc aaatcactgt tctggaaatt
      301 actcagaatg ccctaatatc attaccagaa ttgccagcat cgctggaata ccttgacgcc
      361 tgtgacaatc gcctgtcaac acttcctgaa ttacccgcat ctctgaaaca tcttgatgta
      421 gataacaacc aactaaccat gcttcctgaa ttgcctgcat tgctggaata tattaatgca
      481 gataacaatc agctaaccat gcttcctgaa ttacctacat cgctggaagt gctctcagta
      541 agaaataacc agctgacatt tctccctgag ttacctgaat cactggaagc gctcgatgta
      601 agtactaatc ttctggaaag cctaccagcc gtacctgtaa gaaatcatca ctcagaggaa
      661 accgagatat ttttccggtg ccgcgagaat cgcatcacac acattccgga aaatatactt
      721 agccttgatc cgacctgcac tatcatcctc gaagacaatc ctctgtcctc acggatcagg
      781 gagtctctgt c
//
LOCUS       DAZOYF010000435          782 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZOYF010000435 DAZOYF010000000
VERSION     DAZOYF010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.448"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..666)
                     /locus_tag="ACHUOF_004588"
     CDS             complement(<1..666)
                     /locus_tag="ACHUOF_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFR0613425.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..>782)
                     /locus_tag="ACHUOF_004589"
     CDS             complement(656..>782)
                     /locus_tag="ACHUOF_004589"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HFR0613426.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          251 a          203 c          177 g          151 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttatcaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
      781 tt
//
LOCUS       DAZOYF010000436          778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZOYF010000436 DAZOYF010000000
VERSION     DAZOYF010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.449"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            64..>778
                     /locus_tag="ACHUOF_004590"
     CDS             64..>778
                     /locus_tag="ACHUOF_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0613427.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          259 a          135 c          169 g          215 t
ORIGIN      
        1 ccgaattaac acatgttaac gatttaccag taatgtaaat aaattttcga ggagatcatt
       61 ccagtgggac gtaaatgggc caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg
      121 tctaaaattt atgcaaaatt cggtgtagaa atctatgctg ctgccaaaca aggtgaaccc
      181 gatccagaat taaacacatc tttaaaattc gttattgaac gtgcaaagca ggcacaagtt
      241 ccaaagcacg ttattgataa agcaattgat aaagccaaag gcggcggaga tgaaacgttc
      301 gtacagggac gttatgaagg ctttggtcct aatggctcaa tgattattgc tgagacattg
      361 acttcaaatg ttaaccgtac gattgctaac gttcgcacaa ttttcaataa aaaaggcggc
      421 aatatcggag cggcaggttc tgtcagctat atgtttgaca atacgggtgt gattgtattt
      481 aaaggaacag accctgacca tatttttgaa attttgcttg aagctgaagt tgatgttcgt
      541 gatgtaactg aagaagaagg taatattgtt atttatactg aacctgctga ccttcataaa
      601 ggaatcgcgg cgctaaaagc agctggaatc actgagttct caacaacaga attagaaatg
      661 attgctcaat ctgaagttga gctttcccca gaagatttag aaatctttga agggcttgtt
      721 gatgcccttg aagatgacga cgatgtacaa aaagtttatc ataacgtcgc aaatctct
//
LOCUS       DAZOYF010000437          772 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZOYF010000437 DAZOYF010000000
VERSION     DAZOYF010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.450"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..243
                     /locus_tag="ACHUOF_004591"
     CDS             <1..243
                     /locus_tag="ACHUOF_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0613428.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            358..>772
                     /locus_tag="ACHUOF_004592"
     CDS             358..>772
                     /locus_tag="ACHUOF_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR0613429.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPS"
BASE COUNT          206 a          198 c          201 g          167 t
ORIGIN      
        1 gagctgaaaa ataacggtat aaaggcagta atcccccgaa agtcaaatga gaaaatggca
       61 tcggatggac gtgcacagct tgatcgtgat gcgtactgca atcgtaatgt cgttgaacgg
      121 tgctttgggc ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga
      181 aattacctgg cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat
      241 taagggacac agcctaaata gctgcgcgga atagtagatc cctttgaggg aacttagccc
      301 ggattgtgcg atctgatcaa tcgccaaatc aaaacaaatc accaaccgga ctgagcaatg
      361 ccgatcatag caccaatttc ccgtgacgaa cgacgcctga tgcagaaagc catccataaa
      421 acacacgata aaaattatgc ccgcagactg actgccatgc tgatgctgca ccggggcgac
      481 cgtgtcagcg acgttgccag aacgctctgc tgcgcccgtt cctctgttgg acgctggatt
      541 aactggttca cgcagtcggg tgttgaggga ctgaaatcat tacctgccgg gcgtgcccgt
      601 cgctggccgt ttgagcatat ctgcacactg ttacgtgagc tggtaaaaca ttctcccggc
      661 gactttggct accagcgttc acgctggagt acagaactgc tggcaataaa aatcaatgag
      721 ataaccggct gccagttaaa tgccggaacc gttcgccgct ggttgccgtc tg
//
LOCUS       DAZOYF010000438          759 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZOYF010000438 DAZOYF010000000
VERSION     DAZOYF010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.451"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>759
                     /locus_tag="ACHUOF_004593"
     CDS             <1..>759
                     /locus_tag="ACHUOF_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0613430.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagca
//
LOCUS       DAZOYF010000439          747 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZOYF010000439 DAZOYF010000000
VERSION     DAZOYF010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.452"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            95..>747
                     /locus_tag="ACHUOF_004594"
     CDS             95..>747
                     /locus_tag="ACHUOF_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613431.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKI"
BASE COUNT          265 a          145 c          109 g          228 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaa
//
LOCUS       DAZOYF010000440          743 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZOYF010000440 DAZOYF010000000
VERSION     DAZOYF010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.453"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..158)
                     /locus_tag="ACHUOF_004595"
     CDS             complement(<1..158)
                     /locus_tag="ACHUOF_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR0613432.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="ACHUOF_004596"
     CDS             complement(308..469)
                     /locus_tag="ACHUOF_004596"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613433.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(469..654)
                     /locus_tag="ACHUOF_004597"
                     /pseudo
     CDS             complement(469..654)
                     /locus_tag="ACHUOF_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
BASE COUNT          196 a          216 c          144 g          187 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cct
//
LOCUS       DAZOYF010000441          741 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZOYF010000441 DAZOYF010000000
VERSION     DAZOYF010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.454"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            140..595
                     /gene="csgB"
                     /locus_tag="ACHUOF_004598"
     CDS             140..595
                     /gene="csgB"
                     /locus_tag="ACHUOF_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR0613434.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            636..>741
                     /locus_tag="ACHUOF_004599"
     CDS             636..>741
                     /locus_tag="ACHUOF_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HFR0613435.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
BASE COUNT          224 a          137 c          183 g          197 t
ORIGIN      
        1 cgcgcgggtg agttattaaa aatatttccg cagacatact ttccatcgta acgcagcgtt
       61 aacaaaatac aggttgcgtt aacaaccaag ttgaaatgat ttaatttctt aaatgtacga
      121 ccaggtccag ggtgacaaca tgaaaaacaa attgttattt atgatgttaa caatactggg
      181 tgcgcctggg attgcagccg cagcaggtta tgatttagct aattcagaat ataacttcgc
      241 ggtaaatgaa ttgagtaagt cttcatttaa tcaggcagcc ataattggtc aggctgggac
      301 taataatagt gctcagttac ggcagggagg ctcaaaactt ttggcggttg ttgcgcaaga
      361 aggtagtagc aatcgggcaa agattgacca gacaggagat tataaccttg catatattga
      421 tcaggcgggc agtgccaacg atgccagtat ttcgcaaggt gcttatggta atactgcgat
      481 gattatccag aaaggttctg gtaataaagc aaatattaca cagtatggta ctcaaaaaac
      541 ggcagttgta gtgcagagac agtcgcaaat ggcaattcgc gtgacacaac gttaattacc
      601 attcgacttt taaatcaatc cgatgggggt tttacatgaa acttttaaaa gtagcagcaa
      661 ttgcagcaat cgtattctcc ggtagcgctc tggcaggtgt tgttcctcag tacggcggcg
      721 gcggtaacca cggtggtggc g
//
LOCUS       DAZOYF010000442          710 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZOYF010000442 DAZOYF010000000
VERSION     DAZOYF010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.455"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(223..>710)
                     /gene="sseA"
                     /locus_tag="ACHUOF_004600"
     CDS             complement(223..>710)
                     /gene="sseA"
                     /locus_tag="ACHUOF_004600"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR0613436.1"
                     /translation="RGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLAS
                     HENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAI
                     FFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPV
                     K"
BASE COUNT          177 a          198 c          156 g          179 t
ORIGIN      
        1 ttattaccga acacggcgga aagggccggt gtcgataata cgctaaataa acaataatta
       61 ctctcttttg cttgacaaaa agagagttac tggtgagtat tgttttgctg tgaagcacct
      121 gcgttgctca tgtaacacaa gtcaacctgc tggaaaggcc gcgaaccaga ccagcaataa
      181 aaaaacgcca aattcggcgg ttttttattg cctgtaatat acttatttca ctggctcaac
      241 cggtaaatct gcccgcgcgc cccattcact ccatgcgccg tcgtacagtt tcacgtttgg
      301 cacatccagc gtcgcgagtg ctaacaaaac cacggctgcc gttacaccag agccgcagct
      361 gacgataatc ggtttgtcat agctgacgcc gcgaccaaaa aatatcgcat ccagttcatc
      421 ggtcgttttt agttcgcctt cgcgcaccag ttccgtccac ggaacgttca atgcaccggg
      481 aatatgtccg cgacgtaaac ctgggcgagg ttcatcaact tctgcgttaa aacgtgcagc
      541 cgggcgggca tcaataattt gcgccgtatt ttcatggctt gccaataaca catcggttac
      601 tttcaccact gcttcaggat taaacgcggc gttaaactct ccttccggca gctctactgc
      661 accttcttct aatagcaggt catcgcgctg ccagcctgca agtccccgtt
//
LOCUS       DAZOYF010000443          705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZOYF010000443 DAZOYF010000000
VERSION     DAZOYF010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.456"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..444)
                     /locus_tag="ACHUOF_004601"
     CDS             complement(<1..444)
                     /locus_tag="ACHUOF_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR0613437.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDD"
BASE COUNT          215 a          172 c          131 g          187 t
ORIGIN      
        1 atcatctgga taatcggcat gagcaataac atgcttgccg gtgcgactgt gtacttcaag
       61 aataaagggc aaatgctgac atttttccag cgccgttttc gcttgtttct gttgattatc
      121 tgccagcgca ataaaccagt cgccgccatt catcaaccac aaagacatct gctggaatgc
      181 cagcgcatcc atcgccatct gttcatgatt gcctcttacc gcacgaaccc aatgttgttc
      241 cagtaactgc agacaacgta aactttgcgg cccacgatcg ataacgtctc ccactgagat
      301 aagtaaatct cgccacggat caaaacgaca atgccataat ttgcggcgca actgctcaag
      361 acaaccgtgt atatcgccag aaagccagat atgtcgccat tgatgacccg caattctctg
      421 ataaacgggc gcaggctgtt tcatcaatat tttcctcccg cgctaaagat cacataatct
      481 taacaagaat gttaaaaaac gctggactca gacagtagag tgtgtgttat ggttgactat
      541 aaagtcagcg aaggaaatgc ttctggcttt taacagataa aaagagaccg aacatgattc
      601 ctgttttcgt caacaaacaa taaaaccctt taaaattaat gcgttgaata aatttacgtt
      661 catcttttaa tccctgacac gtaccattgc atattaatac attca
//
LOCUS       DAZOYF010000444          703 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZOYF010000444 DAZOYF010000000
VERSION     DAZOYF010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.457"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..>703)
                     /gene="rfbG"
                     /locus_tag="ACHUOF_004602"
     CDS             complement(<1..>703)
                     /gene="rfbG"
                     /locus_tag="ACHUOF_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HFR0613438.1"
                     /translation="INILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQDDIWYKD
                     KIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPGCTYVIK
                     KETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVG
                     ANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKL
                     RRKKADKIYILLMLIL"
BASE COUNT          225 a          141 c           81 g          256 t
ORIGIN      
        1 ttaaaatcaa cattaataaa atataaattt tatcagcctt tttccgcctt aacttccaaa
       61 aacttaaagc caagatcaaa ttgcctatat aacctttacc caattgatta ttcacaaagc
      121 tatcatcagc taaggcatta gctattttag ttacttcttt tcgataccac ttgtttctca
      181 ccaaaccaag ccgtttgtat tttgctttaa aactaatgtt tgctcccact tggttgtttt
      241 catgctgtct gtacagcatt gttggttttc tatcaatata ccacgaataa tttctcgtcc
      301 gcgcaaatga atataaaaac caatcatgca gacagatatc ttgtgcagca tttttattat
      361 ttatgatgaa cttcttaaat tcaataagcg tttctttttt tataacatat gtacaccccg
      421 gacctgctgc ttcaaaaaaa taatcgaatt gagtttgcga ctgagcctta tcaactaaat
      481 tttttcgccc tgatggataa tatgcaatta cattgctgga ataacaattc gcattattaa
      541 ataccaagca gtcaattgca ttttttattt tatctttata ccaaatatcg tcctgatcac
      601 taaaacagat ataatcatag ttttcaagat cgacatcttt aattaatcta taaaaatttt
      661 tacctgcccc cccaaactta ttgacgcttg gaagtatatt tat
//
LOCUS       DAZOYF010000445          696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZOYF010000445 DAZOYF010000000
VERSION     DAZOYF010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.458"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..673
                     /locus_tag="ACHUOF_004603"
     rRNA            <1..673
                     /locus_tag="ACHUOF_004603"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          164 c          206 g          151 t
ORIGIN      
        1 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
       61 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      121 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      181 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      241 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      301 aactggagga aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca
      361 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      421 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      481 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      541 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      601 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
      661 gatcacctcc ttaccttaaa gaagcgttct ttgtag
//
LOCUS       DAZOYF010000446          685 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZOYF010000446 DAZOYF010000000
VERSION     DAZOYF010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.459"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            52..685
                     /locus_tag="ACHUOF_004604"
                     /pseudo
     CDS             52..685
                     /locus_tag="ACHUOF_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          172 c          173 g          135 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttga
//
LOCUS       DAZOYF010000447          679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZOYF010000447 DAZOYF010000000
VERSION     DAZOYF010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.460"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..251)
                     /locus_tag="ACHUOF_004605"
     CDS             complement(<1..251)
                     /locus_tag="ACHUOF_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFR0613439.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>679)
                     /locus_tag="ACHUOF_004606"
     CDS             complement(272..>679)
                     /locus_tag="ACHUOF_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0613440.1"
                     /translation="HRALSEHKTLSVSWSHYRSTVHKCTFVFSPQVIKQRQFSGPHYK
                     VDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNV
                     YGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
BASE COUNT          155 a          156 c          152 g          216 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 acgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gtacttcggt aatgactcca acttactgat agtgttttat
      661 gttcagataa tgcccgatg
//
LOCUS       DAZOYF010000448          676 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZOYF010000448 DAZOYF010000000
VERSION     DAZOYF010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.461"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..534
                     /locus_tag="ACHUOF_004607"
                     /pseudo
     CDS             1..534
                     /locus_tag="ACHUOF_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          162 a          180 c          163 g          171 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta
      661 tcatattctg aggatt
//
LOCUS       DAZOYF010000449          675 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZOYF010000449 DAZOYF010000000
VERSION     DAZOYF010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.462"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(29..397)
                     /locus_tag="ACHUOF_004608"
                     /pseudo
     CDS             complement(29..397)
                     /locus_tag="ACHUOF_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          205 a          118 c          145 g          207 t
ORIGIN      
        1 ttgtctggag attcaggggg ccagtctaat cagttgtcat gcactcggaa taaatcatac
       61 cgtgaatagt gctgtttttg attgtcagac tattggcgtt ggtattggta gaaataccgt
      121 cgtccagata gtaggtagaa atcacgccgt taacggtagc gttattaatg accgggtaga
      181 tatcgccgtt gtaatagcta tcagcggcat agttgttcca gcctacataa ccatcatagt
      241 aaacgtcatc agcataggtg gcgtcgttgt agtgatggaa agtattgtaa gtttgaccag
      301 aaatatcgtt ggtcgctgca ttagcctgag aggtgatagc cagagtgcaa gccaatgcta
      361 attgtgatac tacaagtttc tttttccagg agtgcatttg tcatccctcc tcagggacgt
      421 aataagttga tccatcaatt atcaatgcat agaaaacata ttatgtttcg cgctaacgat
      481 tatgctgcgt tacagacgaa tggttcaatt attctttccc ctaagtccga atgtggacat
      541 atataattta tggagaaaaa cacgcatcaa attgataaaa ggacgatatt tcagtatgtt
      601 gcagataatg ttatttttga tgcatacgcg ttataaatat caaaaataca aaccagataa
      661 atatcttatc aatat
//
LOCUS       DAZOYF010000450          673 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZOYF010000450 DAZOYF010000000
VERSION     DAZOYF010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.463"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            78..>673
                     /locus_tag="ACHUOF_004609"
     CDS             78..>673
                     /locus_tag="ACHUOF_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR0613441.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTE"
BASE COUNT          234 a           87 c          133 g          219 t
ORIGIN      
        1 ttaaaaaagt aatcagggat gtaaaagtca aactaggatt gaagagcaaa taatgattat
       61 gatgaagggt aattaaattg gattttaaac atcttactca atttaaagat ataattgaac
      121 tggacaagcg ccccgttaaa cttgatgaac gggaaacgtt taatgtctca tggggtattg
      181 atgagaacta ccaggttggg gctgcgattt caattgcttc aattcttgaa aagaataaac
      241 aaaacaaatt tacctttcac ataatcgctg attacttaga caaagagtat attgaattat
      301 tatcacaatt agcaacgaag tatcaaacag taattaaatt atatcatatt gattctgagc
      361 cattgaaggc gctacctcaa tcaaatatct ggccagtatc tatttattat cgtttgcttt
      421 catttgatta tttttctgcg cgattggatt cattattata tcttgatgct gatatcgtct
      481 gtaagggttc attgaacgag ttaatagcat tagagtttaa agatgaatat ggggcagtgg
      541 taattgatgt agatgctatg caaagtaaaa gcgctgagcg tttgtgtaat gaggatttta
      601 acggtagcta ttttaactct ggtgtaatgt atattaattt acgggaatgg ttaaaacaaa
      661 gactaacgga aaa
//
LOCUS       DAZOYF010000451          669 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZOYF010000451 DAZOYF010000000
VERSION     DAZOYF010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.464"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..284)
                     /locus_tag="ACHUOF_004610"
     CDS             complement(<1..284)
                     /locus_tag="ACHUOF_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613442.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFA"
BASE COUNT          241 a          131 c          112 g          185 t
ORIGIN      
        1 gcaaaaaata gtctaaaaaa actcaagtct tgtattttac ttcttttctt tataagaaga
       61 aaaaataaaa taccaatccc ccaaccggta aaaagtatac ccattactat gatagcatag
      121 agtaaacttc tggcaacatc atccaaacct gcaccaacaa cccaccatgg aaatccatag
      181 taaaaagagg ttccccagcc ataagaataa gcactgaccc accctacaca tcccatatag
      241 gcgatgaata atgaagaatt tctaagcaat gaattgtcat tcataagaaa ctaactgata
      301 atgtaaaaaa tattcgagat aaaagttgat cttttaattt tcatgctagg aaattaacaa
      361 taatatcatt gttaatgcat atttatcata tcaaaatact ctgcagaatg ctactatcaa
      421 aaccagacaa gtgaacaaaa cttggtcaac atttagtaac gctagttggt aatgtcttca
      481 atcgatagaa gtttctggta atctactggt attcaaacag ttagggctca tacagcttgc
      541 tggaccttcc ccataaccgc ccgaacgtga aggtctgatg tgttgcaaga gagccggatg
      601 cgggaaatct gcaagtccgg ttcgatgaga gggacgttgt ttttacttgt tgttgatgaa
      661 gactttcat
//
LOCUS       DAZOYF010000452          668 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZOYF010000452 DAZOYF010000000
VERSION     DAZOYF010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.465"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>668
                     /locus_tag="ACHUOF_004611"
     CDS             <1..>668
                     /locus_tag="ACHUOF_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFR0613443.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAZOYF010000453          665 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZOYF010000453 DAZOYF010000000
VERSION     DAZOYF010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.466"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(109..239)
                     /locus_tag="ACHUOF_004612"
                     /pseudo
     CDS             complement(109..239)
                     /locus_tag="ACHUOF_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611212.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            237..320
                     /gene="tnpB"
                     /locus_tag="ACHUOF_004613"
                     /pseudo
     CDS             237..320
                     /gene="tnpB"
                     /locus_tag="ACHUOF_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          191 a          123 c          159 g          192 t
ORIGIN      
        1 attacttcct tatgttatat tttggtaaaa aatttatttt tttacattat agtcggctaa
       61 atgaagttta ctgtaaaaaa acgatacttt ttcgggtggc gcttccaatc agtagaatat
      121 gtgtttttca atgaaggagc cgatgacttt tcgtggattt ccacggaacg cgagaagcag
      181 attgttttac gtgccagccg cttgatgcgg gtgcggaggg tcaggttgtt acgttcaaga
      241 tatagcatgt gctggatgaa gatccctttt ctggtcacct gtttatcttt cgtggccgac
      301 ggggtgacac tgttaaaatg taatgaacgc atcccacaca attaaacacg gagtagtatg
      361 atggttaaaa gggtgaaact ggcaggtgtg gagcctcgca gctttccggc atcatactgc
      421 ccaatacggg atgcataaag cgtcctcaga ctggagactc cacaaatgaa atatacaccg
      481 gttggcgttg atatggcaaa acatgtcatt cagattcact tcatcaatga gcacatgtca
      541 gcgccagtga tataagacgg taatggggat gcggacgatt tcctggactc tcagaagatg
      601 aaacatgttt actgaagaac aaaaaattcg cgctattgag ctttatttca aatatggtaa
      661 gaagc
//
LOCUS       DAZOYF010000454          656 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZOYF010000454 DAZOYF010000000
VERSION     DAZOYF010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.467"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>656
                     /locus_tag="ACHUOF_004614"
     CDS             <1..>656
                     /locus_tag="ACHUOF_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613444.1"
                     /translation="VLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRNLPSGVVP
                     AVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHR
                     YGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELS
                     WWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPL
                     P"
BASE COUNT          144 a          179 c          189 g          144 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcc
//
LOCUS       DAZOYF010000455          656 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZOYF010000455 DAZOYF010000000
VERSION     DAZOYF010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.468"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(151..>656)
                     /locus_tag="ACHUOF_004615"
     CDS             complement(151..>656)
                     /locus_tag="ACHUOF_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR0613445.1"
                     /translation="PVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDL
                     KELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDP
                     STPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKL
                     VERLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DAZOYF010000456          641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZOYF010000456 DAZOYF010000000
VERSION     DAZOYF010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.469"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /gene="csgC"
                     /locus_tag="ACHUOF_004616"
     CDS             complement(<1..143)
                     /gene="csgC"
                     /locus_tag="ACHUOF_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HFR0613446.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLR"
     gene            complement(202..567)
                     /gene="csgA"
                     /locus_tag="ACHUOF_004617"
     CDS             complement(202..567)
                     /gene="csgA"
                     /locus_tag="ACHUOF_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR0613447.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT          166 a          146 c          155 g          174 t
ORIGIN      
        1 tcgcgcaggg acaatatttg tactctgcac agacaagatt gagtaagagt aacttcagga
       61 ataatggtgt acatatcccc ttgctgggtc gtattaaagg ttatctggct ggaaagtgcc
      121 gcaaggagta ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt
      181 ttctgtaata caaatgatgt attaatactg atgagcggtc gcgttgttac caaagccaac
      241 ctgagtcacg ttgacggagg agttcgatgc agtctggtca actgcagcac cgttgccacc
      301 accgaactgt ttaacagtca tttcagaatt tttgccgttc cactgatcaa gagtggcgct
      361 gttaccgaag ccacgttggg tcagatcgat tgagctgtca tctgagccct gaccaacatc
      421 tgcaccatta ccaccgccat gctgggtaat agtcaagtca gagttacggg catcagtttg
      481 cagagcaagt gcagagttac cgccaccgta ctggtaaatg ttcagctctg aatttgggcc
      541 gctattatta ctgaggtgta ctggcaatag cggacactac catttgttct tttttaagca
      601 gccatctgat gatatttttc cctgaaggct gccggggaga t
//
LOCUS       DAZOYF010000457          640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZOYF010000457 DAZOYF010000000
VERSION     DAZOYF010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.470"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..499
                     /locus_tag="ACHUOF_004618"
     CDS             <1..499
                     /locus_tag="ACHUOF_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HFR0613448.1"
                     /translation="KKNGLREKIHVTEGQTPFKSSVTISSGTMVSKEVFEIVGMMDEE
                     LFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGINIPIHSPVRRYYRVR
                     NAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKKHILATLDGIRGITGG
                     GRYNA"
     gene            492..>640
                     /locus_tag="ACHUOF_004619"
     CDS             492..>640
                     /locus_tag="ACHUOF_004619"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR0613449.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNT"
BASE COUNT          222 a           88 c          123 g          207 t
ORIGIN      
        1 caaaaaaaat ggtcttcgag agaaaattca tgtcactgaa ggacagacac cgtttaaaag
       61 ttcagtaaca atctcatccg gaaccatggt ttcaaaagaa gtttttgaga ttgttggaat
      121 gatggatgag gaacttttta ttgattatgt cgatacagaa tggtgtctta gatgcttaaa
      181 ctatggcata ttagttcata tcattcctga tatagaaatg gttcatgcta ttggggataa
      241 gtcagtaaaa atctgtggga ttaacatacc aattcactcg ccagtacgtc gttattatcg
      301 agtaagaaat gcatttcttt tgcttagaaa aaatcatgtg cctcttttac tctctattag
      361 ggaagttgtt ttttctttaa ttcatacgac tttaattatc gcaactcaaa aaaataaaat
      421 tgaatatatg aaaaaacata ttttggctac gctggatgga ataagaggaa ttactggggg
      481 cggtcgttat aatgcatagt tctgatcaaa aaagagtagc tgtacttatg gctacgtata
      541 atggcgaatg ctggattgag gaacaattaa aaagtattat tgaacaaaaa gatgtagaca
      601 tctcaatttt tatatcagat gatttatcaa ctgataatac
//
LOCUS       DAZOYF010000458          636 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZOYF010000458 DAZOYF010000000
VERSION     DAZOYF010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.471"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            88..153
                     /locus_tag="ACHUOF_004620"
                     /pseudo
     CDS             88..153
                     /locus_tag="ACHUOF_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            381..629
                     /locus_tag="ACHUOF_004621"
     CDS             381..629
                     /locus_tag="ACHUOF_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0613450.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DAZOYF010000459          636 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZOYF010000459 DAZOYF010000000
VERSION     DAZOYF010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.472"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            19..492
                     /locus_tag="ACHUOF_004622"
     CDS             19..492
                     /locus_tag="ACHUOF_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0613451.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          128 g          185 t
ORIGIN      
        1 ggagatgaac cctatgcaat gtcctggtta atatctcagt gtgctcatca gtgcactgat
       61 aataaaaaga cggagacaga tgccatttac gataaagttc ggagttctta tttgctttca
      121 tgtattctga aaaaaaacaa aaatgttggg cttattcttc atgcaccatc atttgtttct
      181 gtgtctgaaa aaatcgccag aattgtgatg gcaaattact cccgtaactg gagtaattcc
      241 gagttggcca gtgcggtttt aatgagtgaa tcttctttaa aaagaagaat gtacaaagag
      301 gtcggttcta taagtacatt tgtacataaa ataaagctta cagaagctat caggaaatta
      361 cgaaggacaa atactcccat ttctgtcata tcatctgagt taggttacag ttcaccatcg
      421 tatttttcta aagtcttctt taaatattta aagacatacc ctcaaaacat aaggaagaaa
      481 aatggtcggt aatgtcacca attaggagga cttgcagagc cgctttttta cgatactgag
      541 atcaaacaga cataatctat gcggagattt acagcagggc atggttcaca aatctgacag
      601 cgatgaatta tcggcactgc gggcagaaaa tgctcg
//
LOCUS       DAZOYF010000460          634 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZOYF010000460 DAZOYF010000000
VERSION     DAZOYF010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.473"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(110..634)
                     /locus_tag="ACHUOF_004623"
                     /pseudo
     CDS             complement(110..634)
                     /locus_tag="ACHUOF_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          173 a          152 c          128 g          181 t
ORIGIN      
        1 tgactgcttc agttttaaac tcttcgggat aacgcttacc gctcatgggc acctctcttt
       61 aagccatctt aaatgactct gaggtgtctg ttaaacccgt ggcgattcag gtctgcatag
      121 cattatcaac attcggatgt gcaactacag ctaatgcaac atcatcccag cttgcaatag
      181 tgctatcagc aacaccatct ccgtcagtat ctacggtgac aaagttagat ttaccactat
      241 aatcgctggc attacctgta ttaccgaacc aaccagtagt accttcatca gtccatgaac
      301 cagaggttac ggtagaatct ttaacagtga tggtgttatt aaacacttcg ctgctgttta
      361 cgccagtgct gacagtgttg tcgtcaatat cttcccactc ataaccctga gtcaacgtaa
      421 tacccgcaac gtgagagtta ttagtgatgg acagatcaac ttcctgatcc agagtaatag
      481 ccgtaccaat gttgtaaacg tctacaacat gagtatcagc ggcattattg taagtgccgt
      541 tatatgtgta gtgttcgtag ttgtcgtcga tcgttgaatt atcaacagtc agcgccagac
      601 ggtcatggta gtaatcatcc gcacgatcag caca
//
LOCUS       DAZOYF010000461          632 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZOYF010000461 DAZOYF010000000
VERSION     DAZOYF010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.474"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            276..351
                     /locus_tag="ACHUOF_004624"
     tRNA            276..351
                     /locus_tag="ACHUOF_004624"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:309..311,aa:Ile,seq:cat)
BASE COUNT          181 a          114 c          149 g          188 t
ORIGIN      
        1 tgcgaaaact cttcagctaa tgtaataaag ttatattatt agatacaaaa tcattacaaa
       61 gtatcattga atcgatgata actatagata gtgctaatta ttaacatttg cttgttgact
      121 gcgagtatcg actttgcgaa tggaggcggt gtcgggtttt atgtcgcgtg taatcacgac
      181 ccaaggtgaa tgggaacgca taaaaatctg ttatttacat cataagatca tcatgtttat
      241 tgcgtggttt ataagtattg ttattgcata cttaaggccc tttagctcag tggttagagc
      301 aggcgactca taatcgcttg gtcgctggtt caagtccagc aagggccacc aaatcatcat
      361 tcatgtatca ggaagagtta agggtacaag gggtactcga tggcggtcta tatttcatga
      421 ctaacgccat ttcaaggaag aagtgcggtg ggtggtttcg aatgagggct ggtgttttgg
      481 tacgtggcac gccactgcaa tgtatttatt catcagtact cattcaaaag tacctggaaa
      541 aaatgcccgc tatgccaagc gggcaaattg atcctaccca cgtaatatgg acacaggcct
      601 aagcgaggtt ctggttttca aattgttccg ga
//
LOCUS       DAZOYF010000462          606 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZOYF010000462 DAZOYF010000000
VERSION     DAZOYF010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.475"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            22..>606
                     /locus_tag="ACHUOF_004625"
     CDS             22..>606
                     /locus_tag="ACHUOF_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR0613452.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITR"
BASE COUNT          131 a          163 c          176 g          136 t
ORIGIN      
        1 cggagtcgcc ccgcccttct gatgctcccc cgttttgccg atatttttca gcagggaaac
       61 cgctggctta actggctgga gaaacaaccg gaaggggcag tccgtccggt ggtaattgaa
      121 tcagtgacaa aaatcatggc ctgcgggacc acactgatgg gctacacgca gtggtgttgt
      181 tcatcaccgg actgttgcca tacaaaaaag gtctgcttcc ggtgtaaaag ccgctcctgc
      241 ccgcactgcg gggtgaaggc tggcgcacag tggatacagt atctgctgag cctggtcccc
      301 tactgcccgt ggcagcatat tgtgttcaca cttccctgcc agtactggtc cctggtgttc
      361 cacaaccggt ggttactggc agagatgagc cgcattgcag cggatgtgat actggaaatc
      421 tgccatcagg cagatgtgga gccggggata ttcacggtga tccacacatg ggggcgtgac
      481 cagcagtggc atccgcatat ccatttatcg acaactgccg gtggtgtgac gtcgggccac
      541 acctggaaaa atcttcattt ttacgcccgt aaggtgatga gcatgtggcg ctaccggata
      601 acacgg
//
LOCUS       DAZOYF010000463          603 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZOYF010000463 DAZOYF010000000
VERSION     DAZOYF010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.476"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            143..523
                     /locus_tag="ACHUOF_004626"
     CDS             143..523
                     /locus_tag="ACHUOF_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFR0613453.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT          200 a          110 c          125 g          168 t
ORIGIN      
        1 tatacatact aaaaaatcag atttttacga atattcagat tatcataaaa aatgcaacag
       61 acaggaatgc aataattttg cattatctcg ttgcttgtaa tgcaaatatg cattattgtc
      121 agtgtgtgac aataacagag agatgacgat ggcgaccatt aacgcacgta ttgatgatga
      181 tataaagaac caagcagatg aagtgctgaa gctgatgaat atcagtcaaa ctcaggctat
      241 tgcagctttt tatcagtata ttactgaaca aaagaaactg ccatttgtga tcacctcaat
      301 tgtaaaaacg ccgcatgatt tattacgtga aagtacagac atgttagctg aagctcttgc
      361 agtgatttcc aatctacagg tgtggactga acagcaggat gggattggaa aagcaaagct
      421 aatggagtat taccgccgcc tcgacgcact gtactgttgt gcaaaagaga aaataggtct
      481 tctttcagat aaccgggatg ctgaactagg ctgtgtccct taatcatctg agttatagta
      541 actgtctgtt tacacaacat attgaactat ggctcgctac gaccttcccg atgaggcatg
      601 gac
//
LOCUS       DAZOYF010000464          587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZOYF010000464 DAZOYF010000000
VERSION     DAZOYF010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.477"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..453
                     /gene="ospC2"
                     /locus_tag="ACHUOF_004627"
     CDS             <1..453
                     /gene="ospC2"
                     /locus_tag="ACHUOF_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFR0613454.1"
                     /translation="TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSL
                     GNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFIS
                     NGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          213 a           83 c          120 g          171 t
ORIGIN      
        1 acagataact tcaaaaagat aagactcaga gatatatctt tggaggatgc cattaaagca
       61 tccaattatg aagaaattaa caataaggtg actgataaaa aaatggctca tcaagctctt
      121 gcttattctc taggcaatgc aaaatctgat atggcacttt atttactatc taaatttaat
      181 tttacaaaac aagatatcgc agagatggag aaaatgaaca acaatatgta ttgtgagctg
      241 tatgatgttg agtatttact tagtgaagat agtgccaact ataaagtgct agaatatttt
      301 attagtaatg gattggttga tgtaaacaaa agatttcaaa aagcaaatag tggggacact
      361 atgcttgaca atgcaatgaa aagcaaagat tcaaaaacaa ttgatttttt attaaaaaat
      421 ggagcggtat caggcaaacg atttgggagg tgacctaaaa tatatttagg ttgtgccctg
      481 aatctgacgt tgtcattttt gttatattga tcgatatagg ggatggtgca gaccgttccc
      541 ctgcaacaac acattgcatg tggtgaacgc cccgggaatc ctggaga
//
LOCUS       DAZOYF010000465          581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZOYF010000465 DAZOYF010000000
VERSION     DAZOYF010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.478"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(141..>581)
                     /locus_tag="ACHUOF_004628"
     CDS             complement(141..>581)
                     /locus_tag="ACHUOF_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR0613455.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          147 a          163 c           99 g          172 t
ORIGIN      
        1 caccagcacg tttaacgtcc tgtgccgttt ttcaggtata aaaaaaaccg cataaagcgg
       61 gctctttcaa atgtccatgt ctgctattcg cctcgcggta cagctttgcg aagcgtacaa
      121 gaattgaagc agtttttacg tcaaaaagca ataacttttt tctctatacc aaaagccata
      181 accattggtt tgtacaaaat aaattctgcc acctttagcc aatgctcaat gcgtctttca
      241 caggttctta aactccattt cgggtgtgca tcattcagca gttcagccat tttgcatttg
      301 gtcatccccc gcccctcata ccgttgccga aggacgctaa tcaatcctgg atgctctgcc
      361 agcacctcac ttatgacccg atcaatacat aacgcctctg catcagtaca atgcgccagc
      421 cagctctttt gcttaccgtt aatcatatcc cgcaaaaaag cctcaagttc agacttgttc
      481 agacctgctt ttttcatcct ccggagcgcc tcgttaattg ccgtttttgt cagcttttta
      541 gaggtcaaca tcaggttaaa catattcccc gtcttaccgc c
//
LOCUS       DAZOYF010000466          574 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZOYF010000466 DAZOYF010000000
VERSION     DAZOYF010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.479"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>574
                     /locus_tag="ACHUOF_004629"
     CDS             <1..>574
                     /locus_tag="ACHUOF_004629"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFR0613456.1"
                     /translation="NLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFS
                     GNIRIGRFCSIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITK
                     PENPIIGNDVWIGNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRF
                     DSNVIDELLRIKWWNYNYSDLPDNNKCDDIN"
BASE COUNT          184 a           83 c          117 g          190 t
ORIGIN      
        1 gaatttattt cttttctcaa aaggtgacaa gataacaatt gaagataatg ttattgctga
       61 agaatattca acaatgcctg tgaaaaattt ctccagtgtc ggcgcttttt catttccgac
      121 ctgccatttt tcagggaata tacgtatagg tcgattttgt agtattgcat caaatgtaaa
      181 aataatgggt ggaaatcatc ctttgaatcg ttttactact cacatgatga cttataacgg
      241 tgaatttgat aagtttgcta tgagtgaatt tgaacgtagc tggacattga aaccattcat
      301 taccaaacct gaaaatccga ttataggaaa tgatgtctgg ataggaaatg atgttgtatt
      361 gaaaggtggc atcgcaatag gtgatggtgc tgttattgct gccaattccg ttgttaccaa
      421 agatgtgccc ccatatgcca tagttgcagg agttcctgca aaaataatca gatttaggtt
      481 tgattcaaat gttattgatg aactgttgag aataaaatgg tggaattata actactcaga
      541 tcttccggat aataataagt gtgatgatat aaat
//
LOCUS       DAZOYF010000467          566 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZOYF010000467 DAZOYF010000000
VERSION     DAZOYF010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.480"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..297
                     /gene="pliG"
                     /locus_tag="ACHUOF_004630"
     CDS             <1..297
                     /gene="pliG"
                     /locus_tag="ACHUOF_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR0613457.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="ACHUOF_004631"
     CDS             complement(405..>566)
                     /locus_tag="ACHUOF_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR0613458.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAZOYF010000468          560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZOYF010000468 DAZOYF010000000
VERSION     DAZOYF010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.481"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..375)
                     /locus_tag="ACHUOF_004632"
     CDS             complement(<1..375)
                     /locus_tag="ACHUOF_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR0613459.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQE"
BASE COUNT          150 a          110 c          118 g          182 t
ORIGIN      
        1 ttcttgcgaa acccaaacaa cagaaaattg catccacgtc tttggtgtat gattgctgtt
       61 ccagatctcc attcctttgg gggttttatc ccatttgaat atggttttct gatctattat
      121 ttccgcatca aatgccataa gtgataatgc gatcttgaaa gttgaatctg gtgccatttg
      181 cgttgcacac tttgctttat tgaattgagc aatttcagcg tttgtggatg catcgtaaag
      241 taaaaaacaa ccttcagttc cttcaaataa tggagatgca acagtagaga tatctgttga
      301 tgcactggcg ctgctgtaga taatatttgc aattattaaa aaaatagcga agttgatatg
      361 tattgtgttt ttcataataa gtattggttt ggtaaagggc ttaattttaa cggctaacaa
      421 ttaatgaggc tccgggttcg cccaactttg ttttagggcg actgccctgc tgcgtaacat
      481 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
      541 cccgaggcat agactgtacc
//
LOCUS       DAZOYF010000469          555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZOYF010000469 DAZOYF010000000
VERSION     DAZOYF010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.482"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>555
                     /locus_tag="ACHUOF_004633"
     CDS             <1..>555
                     /locus_tag="ACHUOF_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFR0613460.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETV"
BASE COUNT          164 a          135 c          148 g          108 t
ORIGIN      
        1 tgcgccaact ctgcgtatcc gtgacccgca taaagatgaa aagatggcag caatccataa
       61 agcactggac gaatgcagcg cagagcatcc ggtcttttat gaagatgaag tggatatcca
      121 tcttcatccc aaaatcggtg cggactggca actgcgcgga cagcaaaaac gggtggtcac
      181 gccgggacag aatgaaaaat attatctggc cggagcgctg cacagcggga caggtaaagt
      241 cagctatgtg ggcggcaaca gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct
      301 taaagcgaca taccgtcgtg cgaaaaccat cacgctgatc gtggacaact acattatcca
      361 caaaagccgg gaaacacaga gctggctgaa ggagaacccg aagttcaggg gcatttatca
      421 gccggtttac tcgccatggg tgaatcatgt tgaacggcta tggcaggcac ttcacgacac
      481 aataacgcgt aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag ttcgccattt
      541 tatggaaacc gtcag
//
LOCUS       DAZOYF010000470          554 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZOYF010000470 DAZOYF010000000
VERSION     DAZOYF010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.483"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..>554)
                     /locus_tag="ACHUOF_004634"
     CDS             complement(<1..>554)
                     /locus_tag="ACHUOF_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HFR0613461.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIKW"
BASE COUNT          134 a          120 c          135 g          165 t
ORIGIN      
        1 cccatttaat atcaatctgt tgaaggtgta ttattacatc agtagcatat tccagactgc
       61 cttcctggac gatctgatcc ataacatcct cacgcacttt atatcttgtg catttcattt
      121 caagtgccgt gagtacagcc attactggtg atggtagctg tgttgttttc aggccgtcga
      181 tctgactgat cgcttgggcg acggtgtttt gccattctaa agaacaggtg ctgaagtcaa
      241 atgtggctgt tttattatcc cgttgcgaca cgctacaggc taagcgacgt aattgggtca
      301 atttgtctgc ttgctgaaca ggaactccgg gaaagcggcg atgacttaac gtggtgtgta
      361 caacttcggg cggaaaaacg acagcaatat ctttcgccag ccaaggtaaa acagcatctg
      421 tatttttgtc agccgcacgg gtttctgcta cgaaagaggg ttgttcctga ataatggtgg
      481 ataagtccgc taccttgaga gagtccgtta ccgtttggga aggttgcaaa gaattatctt
      541 tcgcttgctc caca
//
LOCUS       DAZOYF010000471          554 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZOYF010000471 DAZOYF010000000
VERSION     DAZOYF010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.484"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>554
                     /locus_tag="ACHUOF_004635"
     CDS             <1..>554
                     /locus_tag="ACHUOF_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0613462.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEM"
BASE COUNT          125 a          135 c          162 g          132 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgtt
//
LOCUS       DAZOYF010000472          537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZOYF010000472 DAZOYF010000000
VERSION     DAZOYF010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.485"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..487
                     /locus_tag="ACHUOF_004636"
                     /pseudo
     CDS             1..487
                     /locus_tag="ACHUOF_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          124 a          118 c          136 g          159 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaaatgtga ggtagcctga gtttaacgga cactccttcc tgaaatagaa tggcatc
//
LOCUS       DAZOYF010000473          534 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZOYF010000473 DAZOYF010000000
VERSION     DAZOYF010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.486"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(43..330)
                     /locus_tag="ACHUOF_004637"
     CDS             complement(43..330)
                     /locus_tag="ACHUOF_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613463.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
     gene            408..>534
                     /locus_tag="ACHUOF_004638"
     CDS             408..>534
                     /locus_tag="ACHUOF_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0613464.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRW"
BASE COUNT          155 a          130 c          116 g          133 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat cgtcttgaag tggcacactg aatttggcca
      361 cctgaacaga ggtgatatgc tcacctcaga acaacacagg tgctccaatg aaaaaaagaa
      421 attttagcgc agagtttaaa cgcgaatccg ctcaactggt tgttgaccag aaatacacgg
      481 tggcagatgc cgccaaagct atggatgttg gcctttccac aatgacaaga tggg
//
LOCUS       DAZOYF010000474          531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZOYF010000474 DAZOYF010000000
VERSION     DAZOYF010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.487"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            89..>531
                     /gene="alaE"
                     /locus_tag="ACHUOF_004639"
     CDS             89..>531
                     /gene="alaE"
                     /locus_tag="ACHUOF_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR0613465.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQV"
BASE COUNT          116 a          117 c          135 g          163 t
ORIGIN      
        1 tacgctatgc aaaaatgcag atggcaatga gatccactgc tttcatctcc attaacatcc
       61 cattacgctt ttattaagga gcattagcat gttctcaccg cagtcacgct tgcgtcatgc
      121 agttgcagat acgttcgcga tggttgttta ctgttctgtc gtgaacatgt gtattgaagt
      181 tttcctctcc ggaatgagct tcgaacagtc tttttattcc agattggtag cgattccggt
      241 gaacatctta attgcatggc catacggtat gtaccgcgat ctgtttatgc gcgcggcacg
      301 caaagttagc ccgtcgggct ggataaaaaa tctggctgat atcctggctt atgtgacgtt
      361 ccagtcaccg gtgtatgtgg cgatcttgtt agtggtgggc gcagactggc atcagattat
      421 ggcggcggtc agttcaaaca tcgttgtttc gatgttgatg ggggcggttt atggctactt
      481 cctcgattat tgccgccgac tgtttaaagt cagccgttac cagcaggtaa a
//
LOCUS       DAZOYF010000475          529 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZOYF010000475 DAZOYF010000000
VERSION     DAZOYF010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.488"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            381..>529
                     /locus_tag="ACHUOF_004640"
     CDS             381..>529
                     /locus_tag="ACHUOF_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0613466.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          129 a          140 c          115 g          145 t
ORIGIN      
        1 tagggtctct ctataggtct tctctatagg tcttctctat ggtctctcta taggtctctc
       61 tagggtctcc tctaggcatt acccccagaa gctaactcta tggccctcta tggtcctcta
      121 tggccctgta cgtcattctt taggatgccc tgtagtttcg tatagcctga tgcctagaga
      181 gaccctagag agaccctaga gtggctccta gagagaccct cccagtagct ccgcacagct
      241 acatcgtcat catcatagta ttcaatctca aagtcattct gtttgcatcc tgttgtcctg
      301 ctggtctccc ttcgcttggg gactttgagt aggtcagtgg gggtgcgact tcagggtgca
      361 aacaaaaagg aaatcaagcg atgtctcaac aaccacaaca accacaacaa gaaatcagca
      421 tcattaacct cgaccagctt gtgtccatga cctcagttga aattgctgag ctgacaggca
      481 aggagcaccg aaatgtcctt cgtgatattc gtaagatgct cgaagaact
//
LOCUS       DAZOYF010000476          523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZOYF010000476 DAZOYF010000000
VERSION     DAZOYF010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.489"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            233..514
                     /locus_tag="ACHUOF_004641"
     CDS             233..514
                     /locus_tag="ACHUOF_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFR0613467.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          134 a          127 c          123 g          139 t
ORIGIN      
        1 aagatatgcc cttaatgcca ctgaaaaacc acataatttg tacagcaata aaagtcacgg
       61 cctaatatgg ctaacaaaca atcaattgtg atatagttca caaaattaat gaaacaaaca
      121 gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc agagtgtacc acgcctcccg
      181 tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag aggaattgtt tgatggctac
      241 cctcaccact ggcgtggttc ttcttcgctg gcaacttctt agtgccgtaa tgatgtttct
      301 ggccagcaca ctcaacatcc gttttcgtcg gtctgattat gtcgggcttg cggtgatcag
      361 cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg gcatcacaat
      421 ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag agatgaatca
      481 gacaccgcca caatggccaa tgattttgac ctgatacgta gaa
//
LOCUS       DAZOYF010000477          523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZOYF010000477 DAZOYF010000000
VERSION     DAZOYF010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.490"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
BASE COUNT          169 a           78 c          106 g          170 t
ORIGIN      
        1 atttcagagt tggagttcga atgttgacac ctgagtattt cctgctaaaa atagccctgc
       61 tttacggatg gttgtattgc aataacacga aagtagtaag agattaaagg tatctgtcag
      121 cgataactct gcaagtgatg acattttgta aaccaaataa tgtaaatcag caatgtatct
      181 tttcacttaa ttataacaaa tgtggtattc actaagaatc tgatacgagc ttacatcacg
      241 actattttag tctcatcgcg ggatacaggg atgcaccgat ggatctcagc acaccgtaat
      301 gcaatacctg aacaaataga acagtaaagt ggttcagtat taatgaaagc tcttattttc
      361 cgttgggaaa acttaaaata gatgagaagg ctttttgatt tcttatgtga tctcatttcg
      421 agtattgaga aatttgtgat ttttaaattt ttcaataagc cctgtaaggg attggggttt
      481 tatgagaaat aaagagacgt attgtctttg tttaaacggt acg
//
LOCUS       DAZOYF010000478          514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZOYF010000478 DAZOYF010000000
VERSION     DAZOYF010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.491"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..392
                     /locus_tag="ACHUOF_004642"
     CDS             <1..392
                     /locus_tag="ACHUOF_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR0613468.1"
                     /translation="DFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKIINHNLPVKN
                     SAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVFVSAL
                     TGNLGSNLTSSIKAKKNAITILNTLNL"
BASE COUNT          198 a          105 c           93 g          118 t
ORIGIN      
        1 aagacttctc tggagataaa gaaagaaaca acggattaac agaagcatgg ctcgaaagta
       61 gcttaaaaat ttcaccagaa gaacaaattc aattcctgcg taaaattatt aatcacaatc
      121 tcccagttaa aaactcagcc atagaaaaca ccatagagaa catgtatcta caagatctgg
      181 ataatagtac aaaactgtat gggaaaactg gtgcaggatt cacagcaaat agaaccttac
      241 aaaacggatg gtttgaaggg tttattataa gcaaatcagg acataaatat gtttttgtgt
      301 ccgcacttac aggaaacttg gggtcgaatt taacatcaag cataaaagcc aagaaaaatg
      361 cgatcaccat tctaaacaca ctaaatttat aaaaaatcta atggcaaaat cgcccaaccc
      421 ttcaatcaag tcgggacggc caaaagcaag cttttggctc ccctcgctgg cgctcggcgc
      481 cccttatttc aaacgttaaa catcatgagg gaag
//
LOCUS       DAZOYF010000479          511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZOYF010000479 DAZOYF010000000
VERSION     DAZOYF010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.492"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..511
                     /locus_tag="ACHUOF_004643"
                     /pseudo
     CDS             1..511
                     /locus_tag="ACHUOF_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAZOYF010000480          490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZOYF010000480 DAZOYF010000000
VERSION     DAZOYF010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.493"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(149..>490)
                     /locus_tag="ACHUOF_004644"
     CDS             complement(149..>490)
                     /locus_tag="ACHUOF_004644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613469.1"
                     /translation="IHHSGVVNISSDNLQNILSKPREHTLNCLGWRFFVQRSVCVSWK
                     SDFAWTGIMLLFRGEELAFSSAFSPVTIRKIMKCPSGEEDRRKKDPPAKTLGMNSFRY
                     SDCWRVKFLMN"
BASE COUNT          149 a          115 c           95 g          131 t
ORIGIN      
        1 ccccgacaga cctgtgctat cggaaagaaa aaaggccatc agcaaaactc tgatgacctg
       61 aatcaccttt accaatattg tatgaaaaaa cacgcacgac taattgacaa taattttcat
      121 ttccattaaa aaagatcgcg tagcattctt aattcatgag gaactttacc cgccagcaat
      181 ctgagtagcg aaagctgttc atccccaatg tttttgctgg cgggtccttt tttctacggt
      241 cctcctcccc ggaggggcat ttcattattt ttctaatcgt tactggtgag aaggcggaag
      301 aaaaagccag ttcttcgccc ctaaataaca gcatgattcc agtccatgca aaatctgatt
      361 tccagctcac acataccgag cgctgtacaa aaaaccgcca gccaaggcag ttaagagtgt
      421 gttcccgggg tttgcttaga atattttgta agttgtccga agagatattt acaacaccag
      481 aatgatgaat
//
LOCUS       DAZOYF010000481          486 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZOYF010000481 DAZOYF010000000
VERSION     DAZOYF010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.494"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(24..419)
                     /locus_tag="ACHUOF_004645"
     CDS             complement(24..419)
                     /locus_tag="ACHUOF_004645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613470.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSLLNEVA"
BASE COUNT          145 a          115 c          105 g          121 t
ORIGIN      
        1 tcaggaagga gtgtccgtta aactcaggct acctcattta acagacttga ttcatcctca
       61 caaaatataa gtttttcctg gaatgcccat gtctcattga ggttccagat atcaaattcg
      121 aagtcgtgat aacgaaaacg gatcccacca aacttattaa aacgcagttg tttgacgggc
      181 agttcagcca atgtctggaa gagttcctca cgggagcgac cgatgacgat atcaaggtct
      241 gaagagaatc catcgacgcc atacaagcct aagtcacgca ccatcccgcc aaaaatcctg
      301 gccggacagg aacaggccag agtatcaagt aatactctgg ctttcttatg ctcatcacta
      361 agcgtaaaaa aatcggcgat acgctgttca agcagttggg tgtttgagac cacattcata
      421 cagtgatttt agctcatcgt gaaggcgcat taaataacta tccgtcatgc ctgagattgc
      481 gtcggt
//
LOCUS       DAZOYF010000482          484 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZOYF010000482 DAZOYF010000000
VERSION     DAZOYF010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.495"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..484
                     /locus_tag="ACHUOF_004646"
                     /pseudo
     CDS             1..484
                     /locus_tag="ACHUOF_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          177 a           81 c           96 g          130 t
ORIGIN      
        1 gtcgagtaat aatgaatatg acctgtttaa aaatagagaa acctcactgt ggaaacgcaa
       61 agtaccagaa agcgcagcgc tggcaatgac gggaacagta tataaattat ttggtaatcg
      121 cttactcaac gcccgcaatc agttagataa aaatctcctc gaaatggcta tgcaaatggt
      181 ttatggcaaa gatatggtga caaagtccgg ggatatattg attgatatac aattaaataa
      241 ggatggcagt ctccagagta agcactacac acttgatgta ggtgaagtta taaacagtta
      301 taatattgac cctctttgtg aatgcttcaa gtattaatca aacttactta catgataaaa
      361 atcctggtga cgttattagc ctgtatggca ctttagataa cattcccctt gtagttaagg
      421 aatgcacggg taaaaatcat caaattaatg ttaaagccaa attgccacaa gaacgaagtg
      481 aaaa
//
LOCUS       DAZOYF010000483          481 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZOYF010000483 DAZOYF010000000
VERSION     DAZOYF010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.496"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            32..>481
                     /locus_tag="ACHUOF_004647"
     CDS             32..>481
                     /locus_tag="ACHUOF_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR0613471.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFK"
BASE COUNT          154 a           97 c          104 g          126 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 a
//
LOCUS       DAZOYF010000484          475 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZOYF010000484 DAZOYF010000000
VERSION     DAZOYF010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.497"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
BASE COUNT          122 a           95 c           92 g          166 t
ORIGIN      
        1 aattatgtgc gtttttatct gtggttattt agataacatt atctaactgg acactttctt
       61 gtatgtatta tagctaaatg aacagatttt aatatgttgt atggttttca ctctgaaatc
      121 ccctgaattt aatcacgttt cctttactga aggcaatttt aaccgtagta tcaattatta
      181 acccttgggc ttgatatccc tctcctcttc tgggcacggg ggatgtcaga ttacttatta
      241 cagaaaccaa aagaattgta agcgtcatct cagtgtcgtc tatttcaatg tctgatgatt
      301 ggcgatgata gagtcctctt ataacgttaa tggactctat caatgtcaga ttctcttcgc
      361 ccccgcagga ttcgtgcctc ttactctatg actctatgga gtttaagctt gatcttattg
      421 aaaaatctta ccagctaggg gcctgcgtgg cccaactggc cagagagtac gggat
//
LOCUS       DAZOYF010000485          473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZOYF010000485 DAZOYF010000000
VERSION     DAZOYF010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.498"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..473
                     /locus_tag="ACHUOF_004648"
                     /pseudo
     CDS             1..473
                     /locus_tag="ACHUOF_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          104 a          117 c          147 g          105 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgc
//
LOCUS       DAZOYF010000486          472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZOYF010000486 DAZOYF010000000
VERSION     DAZOYF010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.499"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            3..>472
                     /locus_tag="ACHUOF_004649"
     CDS             3..>472
                     /locus_tag="ACHUOF_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR0613472.1"
                     /translation="MDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAZOYF010000487          467 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZOYF010000487 DAZOYF010000000
VERSION     DAZOYF010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.500"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..467
                     /locus_tag="ACHUOF_004650"
                     /pseudo
     CDS             1..467
                     /locus_tag="ACHUOF_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          112 a          133 c          136 g           86 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc agaaaat
//
LOCUS       DAZOYF010000488          467 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZOYF010000488 DAZOYF010000000
VERSION     DAZOYF010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.501"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..139
                     /locus_tag="ACHUOF_004651"
     CDS             <1..139
                     /locus_tag="ACHUOF_004651"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0613473.1"
                     /translation="AGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTS
                     C"
     gene            172..467
                     /locus_tag="ACHUOF_004652"
                     /pseudo
     CDS             172..467
                     /locus_tag="ACHUOF_004652"
                     /inference="COORDINATES: protein motif:HMM:NF014380.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          139 a          104 c          113 g          111 t
ORIGIN      
        1 ggcaggctta aagcctgaca ctatgcgtaa cgtatttcgc tgccatatac cacgatacga
       61 agcaatcatt gctagatatt tggatatgga accagcccag atttggcctt ctcggtatat
      121 ggagaaaaca tcatgttaac ccattgggta acagcgcaag aatgcgtagg aatgtcatgc
      181 tttcctaaac atgaggtgag tattcgccgc aatcttgaaa agcttgcagc caatcatcca
      241 gaactacgtc gcaagcgtac gggttcaaag gctttggaat ttcatatatc agttctacca
      301 gcggcagcac gtgctgagtt gttattaaaa tctgagcaaa tagaaacaag ctaggggtat
      361 tttgaaatcg cccgccccac gctggaagcg cacgattacg atcgtgaggc actgtggagc
      421 aaatgggata aagccagcga ttcccagcgc agacttgctg aaaaatg
//
LOCUS       DAZOYF010000489          465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZOYF010000489 DAZOYF010000000
VERSION     DAZOYF010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.502"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>465
                     /locus_tag="ACHUOF_004653"
     CDS             <1..>465
                     /locus_tag="ACHUOF_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR0613474.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASA"
BASE COUNT          147 a           96 c          102 g          120 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agcta
//
LOCUS       DAZOYF010000490          463 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZOYF010000490 DAZOYF010000000
VERSION     DAZOYF010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.503"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>463
                     /locus_tag="ACHUOF_004654"
     CDS             <1..>463
                     /locus_tag="ACHUOF_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR0613475.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          117 a          109 c          142 g           95 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcc
//
LOCUS       DAZOYF010000491          459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZOYF010000491 DAZOYF010000000
VERSION     DAZOYF010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.504"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..>459)
                     /locus_tag="ACHUOF_004655"
     CDS             complement(<1..>459)
                     /locus_tag="ACHUOF_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFR0613476.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DAZOYF010000492          458 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZOYF010000492 DAZOYF010000000
VERSION     DAZOYF010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.505"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
BASE COUNT          132 a          102 c          107 g          117 t
ORIGIN      
        1 ttaaaaaatg gagcaatatt aggcaaacga tttgagatat gacctaaaag ctcggccaaa
       61 taataatgtc gtcgattatc gaagtcatca tgagtgattc tgtccccaca gtgcagcatc
      121 agcatgacag tgaattgtct ggcgtggtcc atttaatgag atggagatgg tccctctttc
      181 ctaaatagct gtgcctaata ccgctacact tttgccagcc catgtttgcc tccggggaat
      241 gggctgacgg ttccttcaaa atccattgca cgcagttatg tcggagcggg aacttgactc
      301 ctgtctttac gggagtgagg tagcctgagt ttaacggaca ctccttcctg aaatagaatg
      361 gcatcagaag gagccaataa tgagcagaaa aacccaacgt tactctacgg agtttaaagc
      421 cgaagccgtc aaaacggttc ctgaaaatca gctttcga
//
LOCUS       DAZOYF010000493          452 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZOYF010000493 DAZOYF010000000
VERSION     DAZOYF010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.506"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(110..>452)
                     /locus_tag="ACHUOF_004656"
     CDS             complement(110..>452)
                     /locus_tag="ACHUOF_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR0613477.1"
                     /translation="YLLVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGG
                     DVKLLTVLSLAIDEHELANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAF
                     LLTYPVFPLFC"
BASE COUNT          174 a           95 c           91 g           92 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aa
//
LOCUS       DAZOYF010000494          443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZOYF010000494 DAZOYF010000000
VERSION     DAZOYF010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.507"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>443
                     /locus_tag="ACHUOF_004657"
     CDS             <1..>443
                     /locus_tag="ACHUOF_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0613478.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTND"
BASE COUNT          136 a           97 c          115 g           95 t
ORIGIN      
        1 agagagtaaa tcaaaccgcg caattggcgt tgcgctgaat gatactgcat gtcgcgtatt
       61 gaaaaaacaa atcggaaatc atcaccgttg ggtatttgtg tacaaggaaa gctgtaccaa
      121 accagacgga acgaaagcgc caacagtaag gaagatgcgg tatgacgcaa acacagcctg
      181 gaaagcggcg ctgagacggg ctggtattga tgatttcaga tttcacgact tgagacacac
      241 ctgggcaagt tggctggttc aagccggagt cccgttatca gtgttacagg aaatgggagg
      301 ctgggagtct atcgaaatgg ttcgtcgata tgctcacctc gcacctaatc accttaccga
      361 acacgcacgg caaatagact tgattctgaa cccatcggtc ccaaatttgt cccagtcaag
      421 aaataaggaa ggtactaatg atg
//
LOCUS       DAZOYF010000495          443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZOYF010000495 DAZOYF010000000
VERSION     DAZOYF010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.508"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            213..>443
                     /locus_tag="ACHUOF_004658"
     CDS             213..>443
                     /locus_tag="ACHUOF_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR0613479.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAZOYF010000496          442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZOYF010000496 DAZOYF010000000
VERSION     DAZOYF010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.509"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..228
                     /locus_tag="ACHUOF_004659"
     CDS             <1..228
                     /locus_tag="ACHUOF_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393504.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFR0613480.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            complement(241..>442)
                     /locus_tag="ACHUOF_004660"
     CDS             complement(241..>442)
                     /locus_tag="ACHUOF_004660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613481.1"
                     /translation="DKWGRNLRVRKVSNYNPLHQQTSNRVPRYAGNTRRLNWQMVGLT
                     SVSDDFLANYVRPVSIFTDWSN"
BASE COUNT          146 a           93 c           79 g          124 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt ca
//
LOCUS       DAZOYF010000497          437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZOYF010000497 DAZOYF010000000
VERSION     DAZOYF010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.510"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..269
                     /locus_tag="ACHUOF_004661"
     CDS             <1..269
                     /locus_tag="ACHUOF_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0613482.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>437
                     /gene="tnpB"
                     /locus_tag="ACHUOF_004662"
     CDS             266..>437
                     /gene="tnpB"
                     /locus_tag="ACHUOF_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0613483.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKIL"
BASE COUNT          109 a          114 c          123 g           91 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgt
//
LOCUS       DAZOYF010000498          435 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZOYF010000498 DAZOYF010000000
VERSION     DAZOYF010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.511"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
BASE COUNT          134 a           46 c           95 g          160 t
ORIGIN      
        1 aattgtcgtt ttttatctgt gaaggagctt gaatattttg gggtgggggg gcataacaaa
       61 tgatattact gagtacaaat ttagctatat tgggcggatt agttgtgtgg gaagacgaaa
      121 tgtttctctt gaaataagtg aactccctca aggaatgatt gtgtgcttaa atatttaagt
      181 gctggtaagt ttgagaaggc tgacatattt tgcagagcaa acagtgattg acttggatga
      241 tatttttatt ttagtcactc gttgtaattg gagtatgctt tattttattt gaaaatagat
      301 gtattttata cccgcaattt aaactgtaag ccctatgagg ggtatggata ttataagtta
      361 ctctttgacc aagcataact tttggggtta gaaaaataaa tctcttgtta ataattaata
      421 tgattttaaa atcaa
//
LOCUS       DAZOYF010000499          434 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZOYF010000499 DAZOYF010000000
VERSION     DAZOYF010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.512"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            35..434
                     /locus_tag="ACHUOF_004663"
                     /pseudo
     CDS             35..434
                     /locus_tag="ACHUOF_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAZOYF010000500          431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZOYF010000500 DAZOYF010000000
VERSION     DAZOYF010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.513"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..149
                     /locus_tag="ACHUOF_004664"
                     /pseudo
     CDS             1..149
                     /locus_tag="ACHUOF_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(169..>431)
                     /locus_tag="ACHUOF_004665"
     CDS             complement(169..>431)
                     /locus_tag="ACHUOF_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HFR0613484.1"
                     /translation="STGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQANSIKSRR
                     VISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          110 a          104 c          101 g          116 t
ORIGIN      
        1 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
       61 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      121 cccgactgtg tcgccccttc aggctgaggg atccccacaa aattactgct aataaaccag
      181 caccatattt cggtaggttc tggacagatg gtttagaact gtgctcagta ctgttcgtct
      241 taaaataagt ggcgagtgtc gaagaacatt cttcgctaac gtcagatatg agataacccg
      301 ccgggattta atgctgttag cctgatattt caggtgtaac cctttatttt cagcgtgata
      361 accgagtaac cacattacga tagtgctcaa cgttgccagc agactcagaa ccagaattct
      421 tcctgttgaa c
//
LOCUS       DAZOYF010000501          409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZOYF010000501 DAZOYF010000000
VERSION     DAZOYF010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.514"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            155..340
                     /locus_tag="ACHUOF_004666"
                     /pseudo
     CDS             155..340
                     /locus_tag="ACHUOF_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          118 a           97 c          108 g           86 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaagg
//
LOCUS       DAZOYF010000502          399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZOYF010000502 DAZOYF010000000
VERSION     DAZOYF010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.515"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            198..399
                     /locus_tag="ACHUOF_004667"
                     /pseudo
     CDS             198..399
                     /locus_tag="ACHUOF_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT          111 a           90 c           69 g          129 t
ORIGIN      
        1 aataatttgg tgttctcccg atttattgac cgtctaaatg agagtttttg atatataact
       61 atagacagct actataactt tcatctattt attcacagcg cgaatgaaaa ataaaacact
      121 ctgctttaca gtgtttactt tataattaac agtcttatct cactgttcac cctgtaacgt
      181 tcagagagcg tcttcagatg tctatttata aaattcctct tccgctcaat attctggaag
      241 ctgcacgaga gcgtattacc tagacactca acaccctacc ccgcgtttgt gtctcatttt
      301 ctggcggcaa agactctggt ttaatgctgc atctgacagc cgaacttgcc cgacaaatgg
      361 gcaaaaaaat ctgcgttttg tttatcaact gggaggcgc
//
LOCUS       DAZOYF010000503          390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZOYF010000503 DAZOYF010000000
VERSION     DAZOYF010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.516"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(42..>390)
                     /locus_tag="ACHUOF_004668"
     CDS             complement(42..>390)
                     /locus_tag="ACHUOF_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0613485.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           91 c           87 g          120 t
ORIGIN      
        1 ccttcgtgaa gctgttgaag ggtggattct taacggtggc gttaatcaga aatgctcctg
       61 atgtgccaga acaatttatc agcgtgaagc tcatacgctt tgcgctgagc ctctatccag
      121 tcgtgtttat tgtatacgga cattatcccg ccaagttcat gaccgagcat tcgctcagta
      181 acatgaggct cgacattcat ctcggacagc cgtgtaacaa gcgttcttct gaaatcatgt
      241 gttcgccagt aacctagatc catccctccc cttattctat ttacgaatcg gttggctgca
      301 gcgatgctga tcggtttgtt tatgtcttct ccagggaaaa gaacatcatt gtacattgtc
      361 atggcctttt cgagaaaagg cttaatttgt
//
LOCUS       DAZOYF010000504          384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZOYF010000504 DAZOYF010000000
VERSION     DAZOYF010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.517"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(1..252)
                     /locus_tag="ACHUOF_004669"
                     /pseudo
     CDS             complement(1..252)
                     /locus_tag="ACHUOF_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           88 a          123 c           86 g           87 t
ORIGIN      
        1 ctcactgtct ccgttatggc gtacaccaca tcttccagta atcgccgctt caccggactc
       61 aggaccccgt atcaccatcc ggaacccctt atccgccaca tgccaggaga acctttccat
      121 aaactcgtcg ccactcatca caaggttttc ttctcgtttt gtccggtggc tgttgtaacg
      181 caggccaatt tcatcctgac cggcataatg ttccaggcgg ctcatctgca ccggctgctt
      241 ctccagccag ttaagccatc ggtttccctg ctgaaaaata tcggcaaaac ggggaagcat
      301 cagaaaggcg gaataacgcc atccggccag tgaaccgtac cgcattcagg gcaatacatg
      361 ccgtcggcgt tgataccggc agga
//
LOCUS       DAZOYF010000505          375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZOYF010000505 DAZOYF010000000
VERSION     DAZOYF010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.518"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>375
                     /locus_tag="ACHUOF_004670"
     CDS             <1..>375
                     /locus_tag="ACHUOF_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0613486.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAZOYF010000506          374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZOYF010000506 DAZOYF010000000
VERSION     DAZOYF010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.519"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>374
                     /locus_tag="ACHUOF_004671"
     CDS             <1..>374
                     /locus_tag="ACHUOF_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0613487.1"
                     /translation="DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
                     MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRS
                     LKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT           89 a           91 c          121 g           73 t
ORIGIN      
        1 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
       61 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gctcaccatg
      121 aaagcgctgg aaatggcatg ggaaacccgt ggtaagcccg gcggggtgat gttccacagc
      181 gatcagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg ataccagatc
      241 agacagagta tgagccggcg cggaaactgc tgggataaca gcccaatgga acgcttcttc
      301 aggagtctga agaacgaatg gatgccggtg gtgggttacg taagcttcag cgaggcagct
      361 cacgccataa cgga
//
LOCUS       DAZOYF010000507          372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZOYF010000507 DAZOYF010000000
VERSION     DAZOYF010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.520"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..311)
                     /locus_tag="ACHUOF_004672"
     CDS             complement(<1..311)
                     /locus_tag="ACHUOF_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0613488.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAZOYF010000508          362 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZOYF010000508 DAZOYF010000000
VERSION     DAZOYF010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.521"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            91..282
                     /locus_tag="ACHUOF_004673"
     CDS             91..282
                     /locus_tag="ACHUOF_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613489.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT           99 a           90 c           97 g           76 t
ORIGIN      
        1 ggccctcacg gcccgctaac gcggagatac gccccgactt cgggtaaaac cctcgtcggg
       61 accactccga ccgcgcacag aagctctatc atggctgaaa acgggtatgg cttggcaggg
      121 ctggggatgg gtaaggtgaa atctgtcaat cagtaccggc tgacgccggg cttcggcggt
      181 tttactccgg tatcacatgt aaccacagcg tgccgccttc catgccgctg gcgcggcatc
      241 agaataatac aagcagcatt taatgcattt gcaaaagtgt gagtaatatt aagtggtcgt
      301 ttcagggata tcgttaagac ttgagcgata ggaattaaaa ccccaaaaag attaaaaaaa
      361 ca
//
LOCUS       DAZOYF010000509          359 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZOYF010000509 DAZOYF010000000
VERSION     DAZOYF010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.522"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            2..>359
                     /locus_tag="ACHUOF_004674"
     CDS             2..>359
                     /locus_tag="ACHUOF_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613490.1"
                     /translation="MKSLAFDSFKERLESTMAALTFFISTGQCDCDEIAESNFNRMIA
                     YMSNINYDASKLGAPALSFDTYLQDNVKYRVIINNLYGSEFRIRDINENFVGMDFTCE
                     FRPEKMTNLINIKNRLV"
BASE COUNT          115 a           56 c           75 g          113 t
ORIGIN      
        1 tattaaaagc ctggctttcg actcttttaa agaaaggctt gagtctacca tggcggcact
       61 cactttcttt atatcaacgg gtcaatgtga ctgcgatgaa atagcagagt cgaacttcaa
      121 tcgtatgatt gcctatatgt ctaacattaa ctatgatgcc tcaaagctag gagcgccagc
      181 acttagtttt gatacgtatc tgcaggacaa tgttaaatat agagtgataa ttaataatct
      241 ttatggcagt gagtttcgga taagagatat taatgagaat tttgttggga tggattttac
      301 ctgtgaattc cgacccgaaa aaatgacgaa tttgatcaat ataaaaaata ggttggtta
//
LOCUS       DAZOYF010000510          357 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZOYF010000510 DAZOYF010000000
VERSION     DAZOYF010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.523"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            39..>357
                     /locus_tag="ACHUOF_004675"
     CDS             39..>357
                     /locus_tag="ACHUOF_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR0613491.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          112 a           86 c           91 g           68 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacc
//
LOCUS       DAZOYF010000511          353 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZOYF010000511 DAZOYF010000000
VERSION     DAZOYF010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.524"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..353
                     /locus_tag="ACHUOF_004676"
                     /pseudo
     CDS             1..353
                     /locus_tag="ACHUOF_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
BASE COUNT           75 a           90 c          111 g           77 t
ORIGIN      
        1 gtacgcacca gaaaatcatt gatatggcca tgaatggcgt tggatgccgg gcaaccgccc
       61 gcattatggg cgttggcctc aacacgattt tccgccattt aaaaaactca ggccgcagtc
      121 ggtaacctcg cgcatacagc cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca
      181 gtggggatac gtcggggcta aatcgcgcca gcgctggctg ttttacgcgt atgacaggct
      241 ccggaagacg gttgttgcgc acgtattcgg tgaacgcact atggcgacgc tggggcgtct
      301 tatgagcctg ctgtcaccct ttgacgtggt gatatggatg acggatggct ggc
//
LOCUS       DAZOYF010000512          342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZOYF010000512 DAZOYF010000000
VERSION     DAZOYF010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.525"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            2..172
                     /gene="xisR"
                     /locus_tag="ACHUOF_004677"
     CDS             2..172
                     /gene="xisR"
                     /locus_tag="ACHUOF_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFR0613492.1"
                     /translation="MMLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWY
                     NYPKINQFIKDS"
     gene            174..>342
                     /locus_tag="ACHUOF_004678"
     CDS             174..>342
                     /locus_tag="ACHUOF_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFR0613493.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           65 c           83 g           86 t
ORIGIN      
        1 cctgatgctt cgcactggtc tgggtgccag acaaattgaa gcataccgcc agaactgttg
       61 ggtggaaggc ttccacttca aacgagtatc tcctttaggg aagccagaca gtaagcgagg
      121 gattatctgg tacaactatc cgaagataaa tcagtttatc aaagactcat gatatgtcta
      181 aattaccaac aggtgtcgag attcgaggta aatacattcg catctggttc atgtttcgag
      241 gaaaacgatg tcgggaaaca ttgaaaggct gggagattac taacagtaac attaaaaagg
      301 ccgggaattt aagagcgttg atagttcatc aaatcagttc cg
//
LOCUS       DAZOYF010000513          341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZOYF010000513 DAZOYF010000000
VERSION     DAZOYF010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.526"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>341
                     /locus_tag="ACHUOF_004679"
     CDS             <1..>341
                     /locus_tag="ACHUOF_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR0613494.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGR
                     AFPRVVKERPW"
BASE COUNT          100 a           60 c          110 g           71 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccgggacgta
      241 taccggagct gatgcgcgat cttgcaagta tgggacaact tgtgaaatta ccgacgagaa
      301 ggggaagggc cttcccgaga gtggtaaagg agaggccctg g
//
LOCUS       DAZOYF010000514          338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZOYF010000514 DAZOYF010000000
VERSION     DAZOYF010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.527"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..>338)
                     /locus_tag="ACHUOF_004680"
     CDS             complement(<1..>338)
                     /locus_tag="ACHUOF_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0613495.1"
                     /translation="SRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDV
                     IRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRA
                     KSLTFYGING"
BASE COUNT           76 a           96 c           88 g           78 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccgggact
//
LOCUS       DAZOYF010000515          338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZOYF010000515 DAZOYF010000000
VERSION     DAZOYF010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.528"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>338
                     /locus_tag="ACHUOF_004681"
     CDS             <1..>338
                     /locus_tag="ACHUOF_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0613496.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLAD"
BASE COUNT           93 a           79 c           78 g           88 t
ORIGIN      
        1 taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag attcagtagt
       61 cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct ctggcgttaa
      121 aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa atgcgacgtt
      181 tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc aggctaccac
      241 cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta catcggtaac
      301 tcccggtacc gaaacgtcga atattcagtt agcggatt
//
LOCUS       DAZOYF010000516          337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZOYF010000516 DAZOYF010000000
VERSION     DAZOYF010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.529"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..>337)
                     /gene="wzx"
                     /locus_tag="ACHUOF_004682"
     CDS             complement(<1..>337)
                     /gene="wzx"
                     /locus_tag="ACHUOF_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HFR0613497.1"
                     /translation="NLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAIALVGYAGIF
                     DVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSDGIVNYLKIS
                     GVEHSDIQLA"
BASE COUNT          130 a           68 c           55 g           84 t
ORIGIN      
        1 gctagttgta tatcactatg ttcaacacca gaaattttta gataattaac aattccatca
       61 gagaaaatca ataataaaaa agcaccaaag catgaaaata gcaccaaaaa agatgtactt
      121 gttgaaatta ccttggttct ttcatgatga ttatcacgat gaattgcaat ttctctaata
      181 acagagcgag tcaggcctac atcaaaaatt ccagcatacc caacaagtgc aattgcaatt
      241 gtataaacac cgaaaccttc aggccccaaa cctcgagcga gaaatcctag cgaggggata
      301 gcaattaaag ttggtattgc atagccaaaa aggttcc
//
LOCUS       DAZOYF010000517          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZOYF010000517 DAZOYF010000000
VERSION     DAZOYF010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.530"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            51..>332
                     /locus_tag="ACHUOF_004683"
     CDS             51..>332
                     /locus_tag="ACHUOF_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0613498.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DAZOYF010000518          325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZOYF010000518 DAZOYF010000000
VERSION     DAZOYF010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.531"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            103..>325
                     /locus_tag="ACHUOF_004684"
     CDS             103..>325
                     /locus_tag="ACHUOF_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR0613499.1"
                     /translation="MIYSNQLRYTGKDGVYFHLQGQPDNFLSLSATENMEFDSPMMVT
                     TQNMQSGQTVTDNVMRSPRTISISGVVVVG"
BASE COUNT          110 a           56 c           61 g           98 t
ORIGIN      
        1 aatttacgta gctcacgctt aacagagaaa atacttatat atcaaaggct tggttaaaaa
       61 tgattaaaat gcgctaagtc tttgaaatca aaagagaatt taatgattta ctctaatcag
      121 cttcgttaca ctggcaaaga tggtgtttac ttccatctac aaggccaacc agataatttc
      181 ttgtcactat cagcaactga aaacatggaa tttgatagcc ctatgatggt taccactcaa
      241 aatatgcaat cagggcagac tgtaacagat aatgtaatgc gttcacctag aactatttca
      301 atcagtggtg tggttgtggt tggtg
//
LOCUS       DAZOYF010000519          325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZOYF010000519 DAZOYF010000000
VERSION     DAZOYF010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.532"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
BASE COUNT           99 a           47 c           58 g          121 t
ORIGIN      
        1 tttttcatta aaaacaatgt aatagagaat tcttgggtgg tggcaaggtg ttttatgttg
       61 ttatttttat gcgctttctg tgcctcctga attatcacgt aaaaatcagg tattaaaata
      121 tcactattag tacttggtta ttattttgaa cgcatttata aaattattac ataaaaatag
      181 cgaatattgc taaaatcccc gccaacgatg tgttgacggg gctgttatta ttttggcaat
      241 aatactccgg tataagtatt taccggatga gaaagatatt gtttaacggc agtgttaaca
      301 ttctctaccg tcatttgttt caaca
//
LOCUS       DAZOYF010000520          324 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZOYF010000520 DAZOYF010000000
VERSION     DAZOYF010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.533"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..>324)
                     /gene="capU"
                     /locus_tag="ACHUOF_004685"
     CDS             complement(<1..>324)
                     /gene="capU"
                     /locus_tag="ACHUOF_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR0613500.1"
                     /translation="HLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFG
                     MVLAEASAFSVPVVATQIGGIPEVIQNNQTGTLLPASNKHAWMCALNDFFNDPGRFYQ
                     MARLA"
BASE COUNT           99 a           82 c           69 g           74 t
ORIGIN      
        1 ttgccaggcg agccatctga taaaaacgcc cagggtcatt aaaaaaatca ttcagggcgc
       61 acatccatgc gtgcttatta cttgctggta acaatgtccc ggtctggttg ttctgaataa
      121 cctcagggat tccaccaatc tgagtggcca ctacaggcac agaaaatgcc gatgcttctg
      181 ccagcaccat accaaaagat tcgttttctg aaggcagaac caccagactg gcaacccgat
      241 atacgggggc ggcaggaaaa acattgtcag caataaaaac atcatcatgc atgcccatac
      301 tgtcaatctg atactgtaaa tgtt
//
LOCUS       DAZOYF010000521          319 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZOYF010000521 DAZOYF010000000
VERSION     DAZOYF010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.534"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
BASE COUNT          109 a           65 c           53 g           92 t
ORIGIN      
        1 agattatata ttagccattt tgatgagtaa tttaggttat tggtgaacag agtggcgctc
       61 tctgctcgct aactccgcaa gcctgacaaa accgaagtga ataaaaatgc aatataacag
      121 aataaaagaa aacacccctc actggtgctt tctgctgtta atgtacattt tctggcatca
      181 aaaatgctgt aaatgacccg gtcatgacaa acaccgaatt ctctacgctt atagcgttac
      241 ctccccccca aactttcatt agtgaaatta aaggcaagat catatctaat tgatttaatt
      301 gaaaataaat catattggc
//
LOCUS       DAZOYF010000522          315 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZOYF010000522 DAZOYF010000000
VERSION     DAZOYF010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.535"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
BASE COUNT           79 a           73 c           75 g           88 t
ORIGIN      
        1 gcgcggggtg tgcataatac gctttcccgc tacagagtca agcatttatt tttgctttct
       61 ctgccggaat tctcaggaga accccgctga cccggcggcg tgtttgccgt tgttccgtgt
      121 cagtggtggc gcattatagg gagttattcc ggcctgacaa gcgaaaaata taaaaacttt
      181 atcgttcgct cacttttcag gcaaaacatc ttaaatatag tcttttccgt ctaacttata
      241 gacaaaaacg agccccgaag ggctaatgcc gatcagttaa ggatcagttg accgatccag
      301 tggctgtgta agaat
//
LOCUS       DAZOYF010000523          313 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZOYF010000523 DAZOYF010000000
VERSION     DAZOYF010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.536"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
BASE COUNT           86 a           57 c           53 g          117 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atg
//
LOCUS       DAZOYF010000524          304 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZOYF010000524 DAZOYF010000000
VERSION     DAZOYF010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.537"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..261
                     /locus_tag="ACHUOF_004686"
     CDS             <1..261
                     /locus_tag="ACHUOF_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0613501.1"
                     /translation="GYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGD
                     AALTSAMLGRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT           90 a           69 c           80 g           65 t
ORIGIN      
        1 ggctatctgc cgttcagtca ggaagaagca aaactgttct tccaggtcat cgctaaacgt
       61 tacgaaaaga gcgcaatgat cctgacatcc aatctaccgt tcgggcagtg ggatcaaacg
      121 ttcgccggtg atgcagcact gacctcagcg atgctgggcc gtatcttaca ccactcacat
      181 gtcgttcaaa tcaaaggaga aagctatcga ctcagacaga aacgaaaggc cggggttata
      241 gcagaagcta atcctgagta aaacggtgga tcaatattgg gccgttggtg gagatataag
      301 tgga
//
LOCUS       DAZOYF010000525          301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZOYF010000525 DAZOYF010000000
VERSION     DAZOYF010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.538"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            140..>301
                     /locus_tag="ACHUOF_004687"
     CDS             140..>301
                     /locus_tag="ACHUOF_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613502.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGIT"
BASE COUNT           92 a           59 c           58 g           92 t
ORIGIN      
        1 tatgacgcac tgatattatt catcaaataa taacaaaata gccattgcat cgggttggac
       61 aatttacaaa agaaagataa cccatattgt ataatgcgtt taaatatgcc gtcttatata
      121 aaatgatggt cagattaata tgcggattat ctttctacgc aaggagtatt tatctctact
      181 cccgtcaatg attgcatctc ttttctctgc taacggtgtc gcggcggcca ttgatttatg
      241 ccagggatat gatatcaaag cgagttgtca cgccagcagg caaagccttt caggcattac
      301 g
//
LOCUS       DAZOYF010000526          299 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZOYF010000526 DAZOYF010000000
VERSION     DAZOYF010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.539"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>299
                     /locus_tag="ACHUOF_004688"
     CDS             <1..>299
                     /locus_tag="ACHUOF_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR0613503.1"
                     /translation="VIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGV
                     FWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSEN"
BASE COUNT           65 a          100 c           86 g           48 t
ORIGIN      
        1 cgttattcag gcccgccagc gcctgggaag tgaggctgtc cgccgcgtgt tcacgaaaac
       61 agcgcagctc tggcataacg ccacgccgca tccgcactgg tgcggcctga ccctgctggc
      121 catcgatggt gtgttctggc gcacaccgga tacaccagag aacgatgcag ccttcccccg
      181 ccagacacat gccgggaacc cggcgctcta cccgcaggtc aaaatggtct gccagatgga
      241 actgaccagc catctgctga cggctgcagc cttcggcacg atgaagaaca gcgaaaatg
//
LOCUS       DAZOYF010000527          289 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZOYF010000527 DAZOYF010000000
VERSION     DAZOYF010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.540"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>289
                     /locus_tag="ACHUOF_004689"
     CDS             <1..>289
                     /locus_tag="ACHUOF_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR0613504.1"
                     /translation="TLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKG
                     DHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
BASE COUNT           84 a           68 c           89 g           48 t
ORIGIN      
        1 gacgttaatg gataaaggtt attactcact gggactgtta aatggctgga gcctggcggg
       61 agaacaccgc cactggatga tacctctcag aaagggagcg caatatgaag agctcagaaa
      121 actgggtaaa ggcgatcatc tggtgaagct gaaaaccagc ccgcaggcac gaaaaaagtg
      181 gccgggactg ggaaatgaag tgacagcccg cctgctgacc gtgacgcgca aaggaaaagt
      241 ctgccatctg ctgacgtcga tgacggacgc catgcgcttc cccggagga
//
LOCUS       DAZOYF010000528          281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZOYF010000528 DAZOYF010000000
VERSION     DAZOYF010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.542"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..281
                     /gene="ydjO"
                     /locus_tag="ACHUOF_004690"
                     /pseudo
     CDS             1..281
                     /gene="ydjO"
                     /locus_tag="ACHUOF_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
BASE COUNT           81 a           49 c           55 g           96 t
ORIGIN      
        1 atacatcctg ccgggagcat ttttgctgcg attcaaaaaa aatgagagtc tcctaaagtt
       61 tccttcgata atatcaatgc ttaatatcaa tcttcttttt aatccactaa atcttccggg
      121 aatgggaagt ggtgtgcttg aagatattat gagtattcca gatagttcac ttagaaaacg
      181 tctcgggtat gaggttttaa gcttctcact acaggcacat tcattgagcc aggaatgtat
      241 tgataagttg gacatttttt tcgctgacga tctgtttaaa t
//
LOCUS       DAZOYF010000529          278 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZOYF010000529 DAZOYF010000000
VERSION     DAZOYF010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.543"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            24..100
                     /locus_tag="ACHUOF_004691"
     tRNA            24..100
                     /locus_tag="ACHUOF_004691"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHUOF_004692"
     tRNA            143..218
                     /locus_tag="ACHUOF_004692"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZOYF010000530          276 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZOYF010000530 DAZOYF010000000
VERSION     DAZOYF010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.544"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..266)
                     /gene="virK"
                     /locus_tag="ACHUOF_004693"
     CDS             complement(<1..266)
                     /gene="virK"
                     /locus_tag="ACHUOF_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR0613505.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRG"
BASE COUNT           90 a           58 c           56 g           72 t
ORIGIN      
        1 ccacgggtcc aggcgcgccc ccctttatgc aaataagggc gatgaattct tgctggcaac
       61 gtggggtgta ttgccagaat atcctcaatg cacggcaagg aacgcagttc ataataatat
      121 cgaatactga caaccggatt tatagaacat cgcaaaatgt acatgaaccg aaattttctg
      181 tgttcccact tccccggtag tgaatctgag gaaaaaataa tccttttaag gaaaccgata
      241 aatgataagt tacttataga aaacatattg ttactt
//
LOCUS       DAZOYF010000531          270 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZOYF010000531 DAZOYF010000000
VERSION     DAZOYF010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.545"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
BASE COUNT          109 a           39 c           54 g           68 t
ORIGIN      
        1 gaaaggacag tgggattaaa aagtaagaat tgataaaaaa aacgccagca acacatagtg
       61 ccggcggagg gaatacccca tggagaaaat gtgatgccta gagcatcatg aatatataat
      121 taaaaatagt tagcgttgtc aatactttca caaaaataat tttcgtagta tagaaagata
      181 tttttatgca tgacctacct gaatttgctc cgggtagagg ttataaataa aaattgaatc
      241 acgacaaaca caatattcat agtatggcga
//
LOCUS       DAZOYF010000532          270 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZOYF010000532 DAZOYF010000000
VERSION     DAZOYF010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.546"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>270
                     /locus_tag="ACHUOF_004694"
     CDS             <1..>270
                     /locus_tag="ACHUOF_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0613506.1"
                     /translation="VWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLN"
BASE COUNT           68 a           69 c           75 g           58 t
ORIGIN      
        1 gtctggagac tgatgaaacg tgtcggaata aaggctcagg tcggataccg aagcccgcgg
       61 gcacgtaaag gcgaggccag tatcgtgtca cccaacaggc tccagcgaca gttcaatccg
      121 gatgctcctg atgagcgttg ggtaacggac ataacctaca tcaggaccca cgaaggctgg
      181 ctgtatcttg ccgttgttgt tgatctgttc tcacgcaaaa ttatcggctg gtccatgcaa
      241 tcccggatga caaaggacat tgtcctgaac
//
LOCUS       DAZOYF010000533          262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZOYF010000533 DAZOYF010000000
VERSION     DAZOYF010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.547"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..212)
                     /locus_tag="ACHUOF_004695"
     CDS             complement(<1..212)
                     /locus_tag="ACHUOF_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0613507.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETPKSIMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT           77 a           58 c           42 g           85 t
ORIGIN      
        1 taacaggatg ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt
       61 accctcatcg gtaaaaaaat tgcctttcga ccattacgat acattattga ttttggggtt
      121 tcagggaagt aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat
      181 ttgctactaa atagtttctt tattttttcc acgatgcccc caggtttata agtacaaacg
      241 gttatatcca catagagaca aa
//
LOCUS       DAZOYF010000534          258 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZOYF010000534 DAZOYF010000000
VERSION     DAZOYF010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.548"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>258
                     /locus_tag="ACHUOF_004696"
     CDS             <1..>258
                     /locus_tag="ACHUOF_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFR0613508.1"
                     /translation="GKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHD
                     VANQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGEN"
BASE COUNT           67 a           71 c           71 g           49 t
ORIGIN      
        1 ggtaaggtgg aacggatggt gcagtacacc cgtaacagtt tttacatccc actaatgact
       61 cgcctgcgcc cgatggggag cactgtcgat gttgaaacag ccaaccgcca cggtctgcgc
      121 tggctgcacg atgtcgctaa ccaacgaaag catgaaacaa tccaggcccg tccctgtgat
      181 cgctggctcg aagagcagca gtccatgctg gcactgcctc cggagaaaaa agagtatgac
      241 gtgcatcctg gtgaaaat
//
LOCUS       DAZOYF010000535          257 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZOYF010000535 DAZOYF010000000
VERSION     DAZOYF010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.549"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
BASE COUNT           85 a           48 c           59 g           65 t
ORIGIN      
        1 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta
       61 gcacgtcgta tgcaagaacg tgccacggct ggctgatgga tgttcgatag cgcgagtttg
      121 aatgaaaatc agccggagat gattttacat aattgctacg gaattattca atacaggaat
      181 tgcttgcgtc tgcatggatt gacctgaaat attcccgaaa atttctctaa aaaactcgaa
      241 aaaaatggta actggtt
//
LOCUS       DAZOYF010000536          255 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZOYF010000536 DAZOYF010000000
VERSION     DAZOYF010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.550"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>255
                     /locus_tag="ACHUOF_004697"
     CDS             <1..>255
                     /locus_tag="ACHUOF_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR0613509.1"
                     /translation="RKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHDW
                     ANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLY"
BASE COUNT          104 a           37 c           36 g           78 t
ORIGIN      
        1 agaaaatata attgtattta tactataaag tcagaatttg aagaaaaaaa tagtgaatat
       61 tacacccggt ttattaatga tgacactgtc ttcatacatt atactggtat aactaagcca
      121 tggcatgatt gggcgaacta cgcctctgca gattattttc gtaatattta taatatatca
      181 ccatggagaa atatacctta taaaaaagct gttaaaaaac atgagcacaa agaaaaatat
      241 aaacacttgc tttac
//
LOCUS       DAZOYF010000537          255 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZOYF010000537 DAZOYF010000000
VERSION     DAZOYF010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.551"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>255
                     /locus_tag="ACHUOF_004698"
     CDS             <1..>255
                     /locus_tag="ACHUOF_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0613510.1"
                     /translation="SSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLK
                     EAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRA"
BASE COUNT           70 a           62 c           74 g           49 t
ORIGIN      
        1 atcgtccatg gaaacgacat tcgtgctgga tgcactggag caggcgttat gggcccgtcg
       61 accgtccggc acggtccatc acagtgataa aggttctcag tatgtatcgc tggcctacac
      121 acagcggctt aaggaagccg gattactggc atcaacagga agtacaggcg actcgtatga
      181 caacgcgatg gcggagagca tcaatggtct ttacaaagcg gaggtaatac accgtaagag
      241 ctggaaaaac cgtgc
//
LOCUS       DAZOYF010000538          248 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZOYF010000538 DAZOYF010000000
VERSION     DAZOYF010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.552"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>248
                     /locus_tag="ACHUOF_004699"
     CDS             <1..>248
                     /locus_tag="ACHUOF_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0613511.1"
                     /translation="FHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPD
                     EGDLKPDVVKRFEEMVAQAQQSAGAAAGNAQQTAQDVA"
BASE COUNT           61 a           59 c           83 g           45 t
ORIGIN      
        1 gtttccatgc gcggactgga aaatatggtg tgtatctgaa acaggactgg cgcaacgagt
       61 acaacgttgg cgacattgct gtatatgagg actcaaagcc cggcacgctg aatgactttc
      121 tgattgctcc tgatgagggc gacctgaaac cggatgtcgt caaacgcttt gaggaaatgg
      181 tggcgcaggc gcagcagagc gccggggccg cagccggaaa cgcacagcag acggcgcagg
      241 atgtggcg
//
LOCUS       DAZOYF010000539          243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZOYF010000539 DAZOYF010000000
VERSION     DAZOYF010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.553"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>243
                     /locus_tag="ACHUOF_004700"
     CDS             <1..>243
                     /locus_tag="ACHUOF_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFR0613512.1"
                     /translation="RGGMTILRAFIRSLSVPQEQEPAVRFETEPGRQMQVDWGTMRNG
                     RSPLHVFVAVPGYSRMLYIEFTDNMRYDTLETCHRNA"
BASE COUNT           50 a           65 c           66 g           62 t
ORIGIN      
        1 cgtggcggaa tgaccattct cagggcattc attcgttctc tctcggttcc tcaggagcag
       61 gagcctgccg ttcggttcga aactgaaccc ggacgacaga tgcaggttga ctggggcact
      121 atgcgtaatg gtcgctcacc gcttcacgtg ttcgttgctg ttcccggata cagccgaatg
      181 ttgtacatcg aattcactga caatatgcgt tatgacacgc tggagacctg ccatcgtaat
      241 gcg
//
LOCUS       DAZOYF010000540          240 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZOYF010000540 DAZOYF010000000
VERSION     DAZOYF010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.554"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>240
                     /locus_tag="ACHUOF_004701"
     CDS             <1..>240
                     /locus_tag="ACHUOF_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR0613513.1"
                     /translation="EGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVS
                     EKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNE"
BASE COUNT           91 a           31 c           50 g           68 t
ORIGIN      
        1 ttgaaggtgt cgggttaaat gagtatttgg aaatttctga agatctgaag gatcaattat
       61 cagaatcgat aaaagagttg catcagcatg gaatggtgtc tggcgatcct cataaaggaa
      121 attttatagt atccgaaaaa ggtttaagac taattgacct ttcaggtaaa aaaacaaccg
      181 ctgttttaaa agcaaaagat cgcattgatc ttgaacggca ctataatata aaaaatgaat
//
LOCUS       DAZOYF010000541          237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZOYF010000541 DAZOYF010000000
VERSION     DAZOYF010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.555"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..>237)
                     /locus_tag="ACHUOF_004702"
     CDS             complement(<1..>237)
                     /locus_tag="ACHUOF_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0613514.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSR"
BASE COUNT           44 a           80 c           50 g           63 t
ORIGIN      
        1 tcacgactca ggaactgttt caccatttgt gcatagcaca ctttcgccac tggttccggt
       61 ttatccatcc ccagagcacg gtacacctgc ggcagcttct ctccacacac ccggttggca
      121 aggaacccga agtacctcac catcttaaaa aacttctccg ggatgtgctg tttcagcctc
      181 gcgaccagct cacgctgtgt cagcgtttcc gtcgccgttt ctcccgtttt gtggtcc
//
LOCUS       DAZOYF010000542          230 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZOYF010000542 DAZOYF010000000
VERSION     DAZOYF010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.556"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(<1..>230)
                     /locus_tag="ACHUOF_004703"
     CDS             complement(<1..>230)
                     /locus_tag="ACHUOF_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0613515.1"
                     /translation="MSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYI
                     EMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT           56 a           59 c           42 g           73 t
ORIGIN      
        1 gacacttccg agccgttgat aatactggtt ttcatattct gtcggtgaca tctgttcgct
       61 agaaccatgc cgacgcttac tgttataaaa catttcgatg taatcaaaaa tatcactgcg
      121 ggcttcttcc cgcgttccgt agatcttttt ctttatccgt tcacgtttca acaactggaa
      181 aaaactttct gcaaccgcat tatcatggca gttaccgcga cggctcatgc
//
LOCUS       DAZOYF010000543          224 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZOYF010000543 DAZOYF010000000
VERSION     DAZOYF010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.557"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            26..181
                     /locus_tag="ACHUOF_004704"
                     /pseudo
     CDS             26..181
                     /locus_tag="ACHUOF_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT           74 a           50 c           52 g           48 t
ORIGIN      
        1 ctccaacaac ggagacaggt aaattatgga cgaaaagaag cttaaagccc ttgcggctga
       61 actggctaaa ggcctcaaaa ccgaggccga cctcaatcag ttttcccgta tgcagacgaa
      121 attaactgtc gaaacggtgc ttaatgcgga actaactgac catctcgggc atgaaaaaag
      181 ctacatccgg tagtaaacat tatcagtggg ttatgcgata tgat
//
LOCUS       DAZOYF010000544          221 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZOYF010000544 DAZOYF010000000
VERSION     DAZOYF010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.558"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>221
                     /locus_tag="ACHUOF_004705"
     CDS             <1..>221
                     /locus_tag="ACHUOF_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0613516.1"
                     /translation="PTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQS
                     LYDWIQLQRKTLSKHAEMAKAFDYILNHW"
BASE COUNT           68 a           46 c           68 g           39 t
ORIGIN      
        1 tccgacagaa atgactcagg aagcgctcag acggatagca gagttatacg acatagaagc
       61 ggagatacgt ggcagtccgg cagaggaacg gcttgcagtc agaaaagcca gaagcgtcca
      121 gttgatgcag tcgttgtacg actggataca gttgcagagg aaaacgctgt cgaaacatgc
      181 ggagatggcg aaggcgttcg actatatcct gaatcactgg a
//
LOCUS       DAZOYF010000545          218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZOYF010000545 DAZOYF010000000
VERSION     DAZOYF010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.559"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>218
                     /locus_tag="ACHUOF_004706"
     CDS             <1..>218
                     /locus_tag="ACHUOF_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0613517.1"
                     /translation="GKDTHHAVAINRSGKRLFDKALPNDENKLRSLISDLKQHGQILL
                     VVDQPATIGALPVAVARSEGVLVGYLPG"
BASE COUNT           57 a           60 c           49 g           52 t
ORIGIN      
        1 cggtaaagat acgcatcacg ctgttgccat taatcgttca ggtaaacgcc tgttcgataa
       61 agcattaccc aacgacgaaa acaaactcag gtcgctaata tctgacctga aacaacatgg
      121 tcagatactg ctggttgttg atcagccagc taccatcggt gcgttacctg tcgccgttgc
      181 ccgctcagaa ggagtccttg tcggatacct ccctggac
//
LOCUS       DAZOYF010000546          214 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZOYF010000546 DAZOYF010000000
VERSION     DAZOYF010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.560"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..>214
                     /locus_tag="ACHUOF_004707"
     CDS             <1..>214
                     /locus_tag="ACHUOF_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0613518.1"
                     /translation="MRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYM
                     SKKTAGGRNTARYLGRYLKKPPIAASR"
BASE COUNT           54 a           51 c           74 g           35 t
ORIGIN      
        1 atgcggcaac ggcttctgaa agcgtggtca gaggggctgg cgatgccgga gtcgttgtca
       61 catatcacga cggaatcaca gaggagaagc ctggtgctga aagccggagg aaaatactgg
      121 catgtgtaca tgtcgaaaaa aacggccggc gggaggaata cggcgcgcta cctgggtcgt
      181 tatctgaaga agccgccgat agcggcctcc cggc
//
LOCUS       DAZOYF010000547          213 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZOYF010000547 DAZOYF010000000
VERSION     DAZOYF010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.denovo.561"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
BASE COUNT           40 a           67 c           54 g           52 t
ORIGIN      
        1 aggccccttc ctcgggaggg gctttcccgt ttcagcccct tgctgactcc ccgattgttg
       61 atgagcgccg gaccccgcgc aacgctttcc ctgaagcagt tttgccttcc ccgagcggtc
      121 acgcaaaacg ctgcaacgtc ctgagcattt gcacctttta gcggaacgca gatataaaaa
      181 atccccggct ttttgggcca gggatatgtg tta
//
LOCUS       DAZOYF010000548        11893 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.guided.121, whole genome shotgun sequence.
ACCESSION   DAZOYF010000548 DAZOYF010000000
VERSION     DAZOYF010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.guided.121"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            72..1196
                     /locus_tag="ACHUOF_004708"
     CDS             72..1196
                     /locus_tag="ACHUOF_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFR0613519.1"
                     /translation="MTIHYTATLNNKHLSLIALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            1177..3480
                     /gene="trbC"
                     /locus_tag="ACHUOF_004709"
     CDS             1177..3480
                     /gene="trbC"
                     /locus_tag="ACHUOF_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFR0613520.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            4344..5159
                     /gene="sul2"
                     /locus_tag="ACHUOF_004710"
     CDS             4344..5159
                     /gene="sul2"
                     /locus_tag="ACHUOF_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR0613521.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            5220..6023
                     /locus_tag="ACHUOF_004711"
                     /note="aph(3'')-Ib"
     CDS             5220..6023
                     /locus_tag="ACHUOF_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFR0613522.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            6023..6859
                     /locus_tag="ACHUOF_004712"
     CDS             6023..6859
                     /locus_tag="ACHUOF_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR0613523.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(6966..7370)
                     /locus_tag="ACHUOF_004713"
                     /pseudo
     CDS             complement(6966..7370)
                     /locus_tag="ACHUOF_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(7720..8151)
                     /locus_tag="ACHUOF_004714"
     CDS             complement(7720..8151)
                     /locus_tag="ACHUOF_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFR0613524.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(8219..10930)
                     /locus_tag="ACHUOF_004715"
     CDS             complement(8219..10930)
                     /locus_tag="ACHUOF_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFR0613525.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGSGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(10942..11274)
                     /gene="mobA"
                     /locus_tag="ACHUOF_004716"
     CDS             complement(10942..11274)
                     /gene="mobA"
                     /locus_tag="ACHUOF_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFR0613526.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            11507..11842
                     /locus_tag="ACHUOF_004717"
     CDS             11507..11842
                     /locus_tag="ACHUOF_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFR0613527.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     ILVRTKGYTLTDVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
BASE COUNT         2884 a         3334 c         2865 g         2810 t
ORIGIN      
        1 gaaaccaccg aaaccaccga aaccaccgaa accaccgaaa aacagcatcc cgccgacaag
       61 gaaaaagaac aatgaccatc cactacactg caacactgaa taataaacac ctgtccctga
      121 tcgccctgta ccctgacggt aatcgggagg tgattgccat cctgccagtc acagaactgg
      181 gcgcactgat taaccgtaat aacaggctga tgcacctgtc cttcagttcc ccttttcgcg
      241 aaataaccga aacggtgctt gttgataccg ttgatgcaga acgtcccgga catcacatcg
      301 gcagaatgct ctcagcgatt gatgcctgcc tggatgccga atatgccgga aaaggaaaac
      361 atccctcccg gaaaaatggc ttctggcgga cactgatgtg tggctttatc gccggaattg
      421 ctctgccggc gacagttgca ggttaccact ggatgaaggc agagccccct tccacgctgg
      481 cttaccctac atctcaggaa tggagagctg aaccagtaac cgacacagag acaccacgcc
      541 cacatacgga acatcatgtt aacagcatcc cggcccgccc ggctcccaca cagcaggaac
      601 cttcttctgc ccccgccccg acggcagtga cggaagaaca acacgttcag gccgctgagg
      661 cccgccgtaa tctggccaca gtacttaaac gcaacgcaga ccgggggatg ttcaccatta
      721 acctgtccac tggtcatgaa cgcacgctgt acgccttcct tgaccccgca tgcatcaact
      781 gccgtctgct ggaacccgcg cttaagcaac tggcccggga attcagcgtg gtgatttatc
      841 cggtctccgt gattggcggg gagtccagta caaagcggac tgctccgctg ctgtgtgaac
      901 cggactcaca aaaacgggcg accggatggc atcagctgta ttccgctgac agtggtatgg
      961 cccccaccgg cgatgcaccg gccgcacagg atggacagtg tctgaccgcc gcacgtgctg
     1021 ccatcgatgt caataaccag gctttccgcc agttcggttt tggcggaacc ccctgggtgt
     1081 tatcagatac cggctggcac ctgccgtccg gcatcctgaa agacgccact tccgttcgcc
     1141 tcttcctgaa agcaacagat agcgaggagt atcacaatga gccagcacca cgttaatcct
     1201 gacctcatac accgcacggc ctggggaaac ccgttgtgga atgcactgca taacctgaac
     1261 atcactggtc tgtgtctggc cggcagtatc attacggcgc tgatatggcc actggccctt
     1321 cctgtctgcc tgctgttcac cctggtcacc tccgtgatat tcatgctgca acgctggcga
     1381 tgccccctgc gtatgcccat gacactcagc cttgatgacc cctcccagga cagaaaggtc
     1441 aggagaagcc tcttcagctt ctggcccacc ctgtttcagt atgaagcgga tgaaacattc
     1501 cccgcccggg ggatttttta tgtcggttac cgccgtatca acgatatcgg tcgtgagctc
     1561 tggctgagca tggatgacct cacccggcat gtcatgtttt ttgccaccac cggtggcggt
     1621 aaaacagaaa ccacctttgc ctggctgctc aacccgctct gctggggaag aggctttacc
     1681 tttgttgacg gaaaggcaca gaacgatacc acgaggacaa tctggtatct ctcccgacgt
     1741 tttggcaggg aggatgatat cgaagtcatc aacttcatga acggcggaaa atcccgcagt
     1801 gaaatcattc agagcggcga aaaatcccgc ccacagtcaa acacctggaa cccgttcgca
     1861 ttcagcaccg aagccttcac cgccgaaacc atgcagtcca tgctgcccca gaacgttcag
     1921 ggcggtgaat ggcagtcacg ggcgatagcc atgaacaagg ccctggtctt tggcacaaaa
     1981 ttctggtgcg tccgggaagg taaaaccatg tccctgcaga tgttacggga acatatgacg
     2041 ctggaaggga tggcaaaact ctattgccgt ggccttgatg accagtggcc ggaagaagct
     2101 atcgcaccac tgcgaaacta cctgcaggat gtcccgggat tcgatatgtc actggtgaga
     2161 acgccgtcag cctggactga ggagccccgt aaacagcatg cttatctctc cggtcagttc
     2221 tcggaaacgt tcacaacctt tgccgaaacc ttcggggacg tctttgcggc agatgccggc
     2281 gacatcgata ttcgcgacag tattcacagc gaccgcattc tgattgtgat gatccctgca
     2341 ctggacacat cggctcatac cacgtcggca ctcggtcgta tgttcgtcac ccagcagagc
     2401 atgctcctcg cccgtgacct gggctaccgg ctggaaggca cagatgcaca gaccctggaa
     2461 gtcaaaaaat acaaaggcag cttcccctat atctgtatcc ttgacgaagt cggtgcctac
     2521 tacacagacc gcattgctgt ggaggccacc caggtacgtt cactggagtt ttccctgata
     2581 atgacggcgc aggatcagga gcgaattgaa gggcagacct ccgccaccaa caccgccaca
     2641 ctgatgcaga acgccggcac caaattcgcc ggacgcattg tcagtgacga caaaacggca
     2701 agaacggtga aaaatgccgc cggtgaagag gcacgggcca gaatgggcag cctgcagcgc
     2761 cacgatggtg tgatgggtga gtcctgggtg gacggtaacc agatcaccat ccagatggaa
     2821 agcaaaatcg acgtgcagga tttgatcaga ctgaatgctg gtgagttctt taccgttttc
     2881 cagggggatg tggtgccgtc agcctctgtg tatattccgg acagcgagaa aagttgcgac
     2941 agtgatccgg tggtcatcaa ccgctatatt tccgttgaag ccccccggct ggagcgtctc
     3001 cgccgtctcg tccccaggac agtgcagcgc aggctcccca caccggagca tgtcagcagc
     3061 atcatcggtg tactgacagc aaaaccatcc cgtaaacgtc gtaaaaaccg tacagagcct
     3121 taccgtatca ttgatacatt ccagcgccag ttggcaacag cgcagacatc actggatctg
     3181 cttccgcaat atgatacaga tatcgaatcc cgggcaaacg aactgtggaa aaaggctgtg
     3241 cacactatca ataacacaac cagggaagaa cgccgggtat gttatatcac actgaatcgt
     3301 ccggaagagg aacactccgg tcctgaagat ataccttcag tgaaggcgat tctgaatact
     3361 ctgctccccc tggagatgct gttacctgta ccggatctaa ctgcatcccc gccccataaa
     3421 aaaaatgtcg ctcagacacc atcaggtgga aaacaagaga gtcgtaaaaa aagattctga
     3481 taacacctgt atggctgatt ttaataacaa cctttacgcg attattaatc tcctccgctc
     3541 accatagtaa gcggaggatt caggatttca atccatcacc agaggtacaa taaatgtgta
     3601 ttagtagccc ggaaacaaac tcatggtctg ttatttatcg taaaaattcc ggagaagata
     3661 tcaatatcac ttccctgacg tttaaaaata gtcttctggc agcccggaca ctgatggtgc
     3721 ccgaaaacta tatgatatgc atactcagaa acggggagag ggtcagacga tgggacagag
     3781 aaatacttgc aggctcaaac agatggtaca agtgttctcc cgataatttc gaaatactgg
     3841 gtaagctccc cataataaat aaagtcacaa aactgataaa atcataatct acggatttta
     3901 atcagttaag ctaaatcagg atgaacgctt tgattatgaa ttatacatga aaacaaatat
     3961 gggcagcaca aaaaatggtg ccagggttcc ccacgttcgt aatagattca cttctatgga
     4021 tattggtatg aattcccaga tcccactcta ttgtaaacaa gacattttta tcttttatat
     4081 tcaatggctt attttcctgc taattggtaa taccatgaaa aataccatgc tcagaaaagg
     4141 cttaacaata ttttgaaaaa ttgcctactg agcgctgccg cacagctcca taggccgctt
     4201 tcctggcttt gcttccagat gtatgctatt ctgctcctgc agctaatgga tcaccgcaaa
     4261 caggttactc gcctggggat tccctttcga cccgagcatc cgtatgagac tcatgctcga
     4321 ttattattat tatagaagcc cccatgaata aatcgctcat cattttcggc atcgtcaaca
     4381 taacctcgga cagtttctcc gatggaggcc ggtatctggc gccagacgca gccattgcgc
     4441 aggcgcgtaa gctgatggcc gagggggcag atgtgatcga cctcggtccg gcatccagca
     4501 atcccgacgc cgcgcctgtt tcgtccgaca cagaaatcgc gcgtatcgcg ccggtgctgg
     4561 acgcgctcaa ggcagatggc attcccgtct cgctcgacag ttatcaaccc gcgacgcaag
     4621 cctatgcctt gtcgcgtggt gtggcctatc tcaatgatat tcgcggtttt ccagacgctg
     4681 cgttctatcc gcaattggcg aaatcatctg ccaaactcgt cgttatgcat tcggtgcaag
     4741 acgggcaggc agatcggcgc gaggcacccg ctggcgacat catggatcac attgcggcgt
     4801 tctttgacgc gcgcatcgcg gcgctgacgg gtgccggtat caaacgcaac cgccttgtcc
     4861 ttgatcccgg catggggttt tttctggggg ctgctcccga aacctcgctc tcggtgctgg
     4921 cgcggttcga tgaattgcgg ctgcgcttcg atttgccggt gcttctgtct gtttcgcgca
     4981 aatcctttct gcgcgcgctc acaggccgtg gtccggggga tgtcggggcc gcgacactcg
     5041 ctgcagagct tgccgccgcc gcaggtggag ctgacttcat ccgcacacac gagccgcgcc
     5101 ccttgcgcga cgggctggcg gtattggcgg cgctgaaaga aaccgcaaga attcgttaac
     5161 tgcacattcg ggatatttct ctatattcgc gcttcatcag aaaactgaag gaacctccat
     5221 tgaatcgaac taatattttt tttggtgaat cgcattctga ctggttgcct gtcagaggcg
     5281 gagaatctgg tgattttgtt tttcgacgtg gtgacgggca tgccttcgcg aaaatcgcac
     5341 ctgcttcccg ccgcggtgag ctcgctggag agcgtgaccg cctcatttgg ctcaaaggtc
     5401 gaggtgtggc ttgccccgag gtcatcaact ggcaggagga acaggagggt gcatgcttgg
     5461 tgataacggc aattccggga gtaccggcgg ctgatctgtc tggagcggat ttgctcaaag
     5521 cgtggccgtc aatggggcag caacttggcg ctgttcacag cctatcggtt gatcaatgtc
     5581 cgtttgagcg caggctgtcg cgaatgttcg gacgcgccgt tgatgtggtg tcccgcaatg
     5641 ccgtcaatcc cgacttctta ccggacgagg acaagagtac gccgcagctc gatcttttgg
     5701 ctcgtgtcga acgagagcta ccggtgcggc tcgaccaaga gcgcaccgat atggttgttt
     5761 gccatggtga tccctgcatg ccgaacttca tggtggaccc taaaactctt caatgcacgg
     5821 gtctgatcga ccttgggcgg ctcggaacag cagatcgcta tgccgatttg gcactcatga
     5881 ttgctaacgc cgaagagaac tgggcagcgc cagatgaagc agagcgcgcc ttcgctgtcc
     5941 tattcaatgt attggggatc gaagcccccg accgcgaacg ccttgccttc tatctgcgat
     6001 tggaccctct gacttggggt tgatgttcat gccgcctgtt tttcctgctc attggcacgt
     6061 ttcgcaacct gttctcattg cggacacctt ttccagcctc gtttggaaag tttcattgcc
     6121 agacgggact cctgcaatcg tcaagggatt gaaacctata gaagacattg ctgatgaact
     6181 gcgcggggcc gactatctgg tatggcgcaa tgggagggga gcagtccggt tgctcggtcg
     6241 tgagaacaat ctgatgttgc tcgaatatgc cggggagcga atgctctctc acatcgttgc
     6301 cgagcacggc gactaccagg cgaccgaaat tgcagcggaa ctaatggcga agctgtatgc
     6361 cgcatctgag gaacccctgc cttctgccct tctcccgatc cgggatcgct ttgcagcttt
     6421 gtttcagcgg gcgcgcgatg atcaaaacgc aggttgtcaa actgactacg tccacgcggc
     6481 gattatagcc gatcaaatga tgagcaatgc ctcggaactg cgtgggctac atggcgatct
     6541 gcatcatgaa aacatcatgt tctccagtcg cggctggctg gtgatagatc ccgtcggtct
     6601 ggtcggtgaa gtgggctttg gcgccgccaa tatgttctac gatccggctg acagagacga
     6661 cctttgtctc gatcctagac gcattgcaca gatggcggac gcattctctc gtgcgctgga
     6721 cgtcgatccg cgtcgcctgc tcgaccaggc gtacgcttat gggtgccttt ccgcagcttg
     6781 gaacgcggat ggagaagagg agcaacgcga tctagctatc gcggccgcga tcaagcaggt
     6841 gcgacagacg tcatactaga tatcaagcga cttctcctat cccctgggaa cacatcaatc
     6901 tcaccggaga atatcgctgg ccaaagcctt agcgtaggat tccgcccctt cccgcaaacg
     6961 accccaaaca ggaaacgcag ctgaaacggg aagctcaaca cccactgacg catgggttgt
     7021 tcaggcagta cttcatcaac cagcaaggcg gcactttcgg ccatccgccg cgccccacag
     7081 ctcgggcaga aaccgcgacg cttacagctg aaagcgacca ggtgctcggc gtggcaagac
     7141 tcgcagcgaa cccgtagaaa gccatgctcc agccgcccgc attggagaaa ttcttcaaat
     7201 tcccgttgca catagcccgg caattccttt ccctgctctg ccataagcgc agcgaatgcc
     7261 gggtaatact cgtcaacgat ctgatagaga agggtttgct cgggtcggtg gctctggtaa
     7321 cgaccagtat cccgatcccg gctggccgtc ctggccgcca catgaggcat gttccgcgtc
     7381 cttgcaatac tgtgtttaca tacagtctat cgcttagcgg aaagttcttt taccctcagc
     7441 cgaaatgcct gccgttgcta gacattgcca gccagtgccc gtcactcctg ccagacatgc
     7501 tgattaattt tgcccttatt aacatcacag acagaaagaa cgagggtacc aataccattg
     7561 atggtaactg gcaggccgat gaaggacgcc gttaccggga taatgtaaga atctattttt
     7621 gagtatgtca acataacctg aggcaaagta cctcaggttc cagtgtagta atcagccaac
     7681 ctgacagtct tctgttagcc ctacagatat gtcgtggagc taaccgcgca tgtcaaagcc
     7741 ggatttttga aaatctctat aaacttccgg attgttgaag gccgggaatt tagctttatg
     7801 ggctgctgac tttatcgctt cgcaataggc tttatcgcca ttactggttg atatttttaa
     7861 cgccgtgcca tcccgggaga attccatatg caacctgcat tttttccttt tccatttttg
     7921 cggatcatca agtttagcat taattgcatc cctgattccc cgtgcttgct ccccccattc
     7981 atcctgatca tcccaacgtc ctgaactgca actacccagg gctgtagttt tatggcaatt
     8041 tgaaggatgt aatggcgcac atcccgccac aaagctgact aaaatagcca gcataacgat
     8101 tttctttacc cccatatatt gctcctcaat ccatatagat ctcagtacca tagctctgtc
     8161 agagcaaaga aaatcttaac taatacaagt taagattttt aatatccact cctcatccct
     8221 acattccagg tgatggtggc gtccactcat tatcctgaac tggagcataa gatgcgccat
     8281 gccccccgga tatgcttttc tgttgcctga tatattcgtg gtagtactgt tcactacgct
     8341 gacgatgtaa atccacatcc gggcggctga ttacaaagtg tccacttccg gttattccag
     8401 ctgcattatg ccaggccacc atttccgcaa atacatcatt aaactgttga tatgagcctt
     8461 ccggttcaaa acccattcct ggctcacgat caaacaatac aggagcaatc agattcagct
     8521 tatccaccag ttcatttctg tcctgatgat ggtagaacac cacacggtcc ccataatcca
     8581 cacagactaa ctcgccattc ctccgatcac gaaaacgcac actgccatct ttctgcagac
     8641 gggcttcgag aacgccagtg tcttcataaa gtggagttcc tcccggttca cagagaatca
     8701 cgcagcggtc ggcattcgtt gttcgtcgct tgtctttgcg acgatcacga taatcccagc
     8761 cacgtaactg tgataatgca gctctgtctc cctgtaccgc ctgttgctcc acccactgcc
     8821 ggtaggacaa aggataccac ttaccctccg caatcagtga caaccgctct tccttcacac
     8881 tctccttcag cctgatcagc gcctgcatac gctgcacttc tgcaatatgg taatgcagct
     8941 tacgcaattg tggatcccgg aactgcacac ggatatacgc cttgcgccga cggcaggcag
     9001 catgaatttc ccgtaaccgt tctccgtaac gtaagtcggg tttacgccag tgctccttcc
     9061 atgcaagata acgcgcgcgc aaatcttccc gcgcagctgc acgggcttca cggcgttcac
     9121 gacgcagtac aggatcgcgc cggaagccgg gctccacttc atcgctggca gcaagttccg
     9181 gattgtagcg acattcaggc ttcacccgtt caaatatatc tgcacctgca atctcaaatg
     9241 gccctgcctg cggctcagcc ctggataacg tcaaatccgg gtggatactg ctggccttca
     9301 ctggtgtgag ctcatgatta aacgcatcca caatcacaag accatgatgc tgtttctgca
     9361 acataagccc tttacgcgca aacatctcat ggcacgcctg ccagctgaaa ttcccgcccg
     9421 cgatcatatc ttcaatcaac gactcacact gaacagtcgt gtacccccac agactttcct
     9481 tatcagaaaa cagttcgcgc tttattgctc cctgtggacc tgtccccatc cgcatacgat
     9541 accattcgta atcactgaca gcctgagtac caagattacg tccccggaat cgttctccgg
     9601 gtataacctg agtaaaaata tccttcggta caggctgcca tccaccatcc agatcctgca
     9661 tataatccag cgtcattaac ggccacacac tgtctgcacg tactggtgtc tgttgtttat
     9721 caaatccatc atgcagtacc agatgcccgt gctggatccc catccataat ccagcctttg
     9781 ccagagttct gtgcacatcc aggcaacttt ccagctcacc atttctcgcc atttccggta
     9841 agcgttcggc aaaatgacgg caggcgtact gtttcagact ctccgtttct gccacttttt
     9901 ccggccggat atcaacgttt atggcttccg gatcataacg accgggtgtc cccacctgac
     9961 tgaaaatatc tgtcggtaca ggctgatatt cccccagttt ccgccccagt ttttcagctg
    10021 tccatgacgg tccgaacgaa ctgagtgcaa caccttcccg cgctctgtcc cagccatcct
    10081 tcaccaccag ttccccttcc tgcatcgtga tatacagacc atcactggcc agccgtttat
    10141 gcaaagacaa ccagtcttgt gcgggctctt cacgcagccc ggcaatcgac atctgcgcaa
    10201 tatattccct gaacgtctgc tttttcccgc gtcgccaggc attacggcgc tcacgtacca
    10261 gcgctgtttt gcgcacaata cggttgccgg gtgcatgcac aaagcagcca ttatcaggcg
    10321 cgaatccgtg cttaagttca agttcacgac atgcacggct gagtttttcc tggctccagg
    10381 aaagccagtt aatataacca gtttcaggat gtacacgatt cacggcaaca tgcacatgca
    10441 gattatccgt gtccgtatga acggcagcca cgtactgatg ttttgagagt tcaagagatt
    10501 tcagtgtgtg acgcacacag tcaaaaagct gctcaggacg cgggctttca tgcgcaggcc
    10561 aggaaagaat gtagtggaaa acggggtctg tatcattacg ggaaaaaaca gctttgtccg
    10621 ccttgtactg catctcttca gcagctgttt ccagtgaatt gcagttatgg aaacaggtta
    10681 cgccatagaa atttacccac tgactgccgt ctttcatcac atcaatcagg gagacaaatt
    10741 tttcgttacg caggcgtgtt gcataatcaa caagccgtct gaaacggttg cggtgaggga
    10801 catcactctt gatttcgctg tcttctgtga gctcattttc ctgcacatca tcccgcacag
    10861 acgtgtacgc aatcaggtct tcaaaggaag acctgccgtc acgtcttttg tgcggaataa
    10921 tggcattcat cagcagttat ctcagcgtcc cattaagtct cttcgcagag cattgacata
    10981 ttcagtaatg gcaacaagaa cctctgcata ctccttatca ccagtgcgtt ttccctccac
    11041 aaacagatgc ttctgaaggc ggccaagacg cataatctca tcaatcatac ggtcgtcagt
    11101 acggcagtta accaccttat ccagtgaaga acttcgtata aatgttgaaa ccgtctgtcc
    11161 acttgactcc gctttttctc ttacgatttc agcctcatcc tccgtgaatc tcgccgtgaa
    11221 ttttacaatt ctcttcctgt tctcactgcc cgttcgggtt ttcttctcac tcatggtctg
    11281 ctccttaccg gggtgtcggg gcaaagccct gaccaggtgg ggaatgtctg agtgcgcgtg
    11341 cgcggtccga cattcccaca tcctgtcccg tttttcagtc cattatagcc tgttattttt
    11401 gtgcatcata gtcttatgca gacggcagag gcagggcgca gccctgccag gagtcagttt
    11461 ttgatgataa aatcaggctt caacattaaa cggagagaaa gtgccaatgg cgtctaaaaa
    11521 attttactca gatgatgaca tccagctggc aaaagctgct ttatcggaat tgccagacct
    11581 caccgctcaa cggaaaacat tgcgtgattt tcttgatgcc attcgtgatg acatcattat
    11641 cctggtaagg acaaagggtt atacactgac agatgttcgt gacacgctca aaaatgcagg
    11701 atatgaagta ggagagaaag cacttcgtga cattattcgg gaagcagaat cgaaaaaacc
    11761 atcccgccgt tcttcctcta aaacagcacc taaaaagaca gactcaggca ggaaagatgg
    11821 catcgatatg aacaacaatt gatgtgacaa tgtcacattg tgttctgaga ttatgatgat
    11881 tacagaaaaa aaa
//
LOCUS       DAZOYF010000549         6976 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.guided.192, whole genome shotgun sequence.
ACCESSION   DAZOYF010000549 DAZOYF010000000
VERSION     DAZOYF010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.guided.192"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            59..1102
                     /locus_tag="ACHUOF_004718"
     CDS             59..1102
                     /locus_tag="ACHUOF_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR0613528.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            1164..1778
                     /gene="prfH"
                     /locus_tag="ACHUOF_004719"
                     /pseudo
     CDS             1164..1778
                     /gene="prfH"
                     /locus_tag="ACHUOF_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACHUOF_004720"
     CDS             complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACHUOF_004720"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0613529.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3554..4012
                     /gene="gpt"
                     /locus_tag="ACHUOF_004721"
     CDS             3554..4012
                     /gene="gpt"
                     /locus_tag="ACHUOF_004721"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0613530.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4104..5348
                     /gene="frsA"
                     /locus_tag="ACHUOF_004722"
     CDS             4104..5348
                     /gene="frsA"
                     /locus_tag="ACHUOF_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0613531.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5406..5807
                     /gene="crl"
                     /locus_tag="ACHUOF_004723"
     CDS             5406..5807
                     /gene="crl"
                     /locus_tag="ACHUOF_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0613532.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="ACHUOF_004724"
     CDS             complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="ACHUOF_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR0613533.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         1689 a         1728 c         1802 g         1757 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggttc atagcggctc gagggggcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
      901 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
      961 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
     1021 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
     1081 atgtcagggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
     1141 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
     1201 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
     1261 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
     1321 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
     1381 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
     1441 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
     1501 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
     1561 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
     1621 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
     1681 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
     1741 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
     1801 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
     1861 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
     1921 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
     1981 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
     2041 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
     2101 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
     2161 tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta
     2221 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
     2281 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
     2341 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
     2401 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
     2461 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
     2521 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
     2581 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
     2641 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
     2701 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
     2761 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
     2821 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
     2881 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
     2941 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
     3001 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
     3061 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggttgac
     3121 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
     3181 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
     3241 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
     3301 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
     3361 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
     3421 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3481 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3541 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3601 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3661 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     3721 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     3781 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     3841 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3901 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
     3961 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     4021 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     4081 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     4141 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     4201 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     4261 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     4321 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
     4381 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     4441 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     4501 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     4561 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     4621 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     4681 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     4741 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
     4801 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4861 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4921 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
     4981 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     5041 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     5101 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
     5161 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     5221 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     5281 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     5341 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
     5401 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     5461 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     5521 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     5581 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     5641 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     5701 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     5761 aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg
     5821 gatgcatcac atccggcaat attcattaaa actgatacgt catcccaacc gcgacagtat
     5881 catcattatt aatattcagc ttattatcgc tatccagttg gttgatttta taatcaacaa
     5941 acgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat
     6001 cttcatcacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     6061 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     6121 agccgccagt tattggcgtc attttgcgtg tttcagaata gaaagttgcc agataaatat
     6181 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
     6241 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
     6301 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
     6361 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
     6421 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
     6481 cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat atcggtccag gcttccacgt
     6541 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
     6601 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctccgcttta tttccggcaa
     6661 actccgcttc ccaccgacca taaccagtca gttgatcgtt aatttgagtt tcacctttaa
     6721 aaccaaaacg gatataactc tggtcgccat ctttactggc gttatcactc atataatgca
     6781 tggctttaac tttgccatag acatccagtt tattaccgtc tttattatat atttctgcag
     6841 cctgtacaga tgcagatgcc acaatgccca tcaccactaa tgccagagtg ctctttttca
     6901 ttttcattcc tgattttaat taacgcgcga atattcagcg ggagagcccc gttgaaaaca
     6961 ggaaagtttt taacct
//
LOCUS       DAZOYF010000550         4970 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.guided.234, whole genome shotgun sequence.
ACCESSION   DAZOYF010000550 DAZOYF010000000
VERSION     DAZOYF010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.guided.234"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            complement(195..845)
                     /gene="tetR(A)"
                     /locus_tag="ACHUOF_004725"
     CDS             complement(195..845)
                     /gene="tetR(A)"
                     /locus_tag="ACHUOF_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFR0613534.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            951..2150
                     /gene="tet(A)"
                     /locus_tag="ACHUOF_004726"
     CDS             951..2150
                     /gene="tet(A)"
                     /locus_tag="ACHUOF_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFR0613535.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2182..2646)
                     /locus_tag="ACHUOF_004727"
                     /pseudo
     CDS             complement(2182..2646)
                     /locus_tag="ACHUOF_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            complement(2679..>4970)
                     /locus_tag="ACHUOF_004728"
     CDS             complement(2679..>4970)
                     /locus_tag="ACHUOF_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="HFR0613536.1"
                     /translation="FGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVLV
                     AFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPH
                     LLNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHIT
                     RRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTD
                     PQEAIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLS
                     KMISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIG
                     LEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASA
                     DGMRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEG
                     LLEQETGLNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGV
                     LNDIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEV
                     GRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQ
                     LGTLGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFT
                     LAELVTKGHLRPLKEASEAENVA"
BASE COUNT         1016 a         1451 c         1410 g         1093 t
ORIGIN      
        1 gcggacgccg cagcaggagc gccagaaggc cgccagagag gccgagcgcg gccgtgaggc
       61 ttggacgcta gggcagggca tgaaaaagcc cgtagcgggc tgctacgggc gtctgacgcg
      121 gtggaaaggg ggaggggatg ttgtctacat ggctctgctg tagtgagtgg gttgcgctcc
      181 ggcagcggtc ctgatcaatc gtcacccttt ctcggtcctt caacgttcct gacaacgagc
      241 ctccttttcg ccaatccatc gacaatcacc gcgagtccct gctcgaacgc tgcgtccgga
      301 ccggcttcgt cgaaggcgtc tatcgcggcc cgcaacagcg gcgagagcgg agcctgttca
      361 acggtgccgc cgcgctcgcc ggcatcgctg tcgccggcct gctcctcaag cacggcccca
      421 acagtgaagt agctgattgt catcagcgca ttgacggcgt ccccggccga aaaacccgcc
      481 tcgcagagga agcgaagctg cgcgtcggcc gtttccatct gcggtgcgcc cggtcgcgtg
      541 ccggcatgga tgcgcgcgcc atcgcggtag gcgagcagcg cctgcctgaa gctgcgggca
      601 ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg gcaccgaatg cgtatgattc
      661 tccgccagca tggcttcggc cagtgcgtcg agcagcgccc gcttgttcct gaagtgccag
      721 taaagcgccg gctgctgaac ccccaaccgt tccgccagtt tgcgtgtcgt cagaccgtct
      781 acgccgacct cgttcaacag gtccagggcg gcacggatca ctgtattcgg ctgcaacttt
      841 gtcatgcttg acactttatc actgataaac ataatatgtc caccaactta tcagtgataa
      901 agaatccgcg cgttcaatcg gaccagcgga ggctggtccg gaggccagac gtgaaaccca
      961 acagacccct gatcgtaatt ctgagcactg tcgcgctcga cgctgtcggc atcggcctga
     1021 ttatgccggt gctgccgggc ctcctgcgcg atctggttca ctcgaacgac gtcaccgccc
     1081 actatggcat tctgctggcg ctgtatgcgt tgatgcaatt tgcctgcgca cctgtgctgg
     1141 gcgcgctgtc ggatcgtttc gggcggcggc cggtcttgct cgtctcgctg gccggcgctg
     1201 ctgtcgacta cgccatcatg gcgacggcgc ctttcctttg ggttctctat atcgggcgga
     1261 tcgtggccgg catcaccggg gcgactgggg cggtagccgg cgcttatatt gccgatatca
     1321 ctgatggcga tgagcgcgcg cggcacttcg gcttcatgag cgcctgtttc gggttcggga
     1381 tggtcgcggg acctgtgctc ggtgggctga tgggcggttt ctccccccac gctccgttct
     1441 tcgccgcggc agccttgaac ggcctcaatt tcctgacggg ctgtttcctt ttgccggagt
     1501 cgcacaaagg cgaacgccgg ccgttacgcc gggaggctct caacccgctc gcttcgttcc
     1561 ggtgggcccg gggcatgacc gtcgtcgccg ccctgatggc ggtcttcttc atcatgcaac
     1621 ttgtcggaca ggtgccggcc gcgctttggg tcattttcgg cgaggatcgc tttcactggg
     1681 acgcgaccac gatcggcatt tcgcttgccg catttggcat tctgcattca ctcgcccagg
     1741 caatgatcac cggccctgta gccgcccggc tcggcgaaag gcgggcactc atgctcggaa
     1801 tgattgccga cggcacaggc tacatcctgc ttgccttcgc gacacgggga tggatggcgt
     1861 tcccgatcat ggtcctgctt gcttcgggtg gcatcggaat gccggcgctg caagcaatgt
     1921 tgtccaggca ggtggatgag gaacgtcagg ggcagctgca aggctcactg gcggcgctca
     1981 ccagcctgac ctcgatcgtc ggacccctcc tcttcacggc gatctatgcg gcttctataa
     2041 caacgtggaa cgggtgggca tggattgcag gcgctgccct ctacttgctc tgcctgccgg
     2101 cgctgcgtcg cgggctttgg agcggcgcag ggcaacgagc cgatcgctga tcgtggaaac
     2161 gataggccta tgccatgcgg gtcaaggcga cttccggcaa gctatacgcg ccctaggagt
     2221 gcggttggaa cgttggccca gccagatact cccgatcacg agcaggacgc cgatgatttg
     2281 aagcgcactc agcgtctgat ccaagaacaa ccatcctagc aacacggcgg tccccgggct
     2341 gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc gagatccccc ggaaccaaag
     2401 gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc aggccgagaa cattggttcc
     2461 tgtaggcatc gggattggcg gatcaaacac taaagctact ggaacgagca gaagtcctcc
     2521 ggccgccagt tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc acttgcgggt
     2581 cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga cgccgacagg
     2641 atctaggggt ctgacgctca gtggaacgaa aactcacgtt aagcaacgtt ttctgcctct
     2701 gacgcctctt ttaatggtct cagatgtcct ttggtcacca gttctgccag cgtgaaggaa
     2761 taatggccga gcatattgat atgtccgtgg caaagcgggg agaggcgtgc gatatcttca
     2821 tcattcagtg tttcaccctg cgcccggaga tgatccaggg ctgcctgcat ataaatagtg
     2881 ttccataaca cgacggcgtt agtgaccagc cccagtgtgc ccagttgatc ttcctgaccg
     2941 tcggtatatc gttttcttat ctcacctttt tgaccgtgac agatggctct ggcaacggca
     3001 tggcgacttt ctccccgatt aagctgggtc agaatgcgcc ggcggtaatc ttcatcatca
     3061 atataattaa gcagatacag cgttttgttg atgcgcccca cttcaatgat tgcctgagtc
     3121 agtccggaag gacgttcact tttcagcaat gaacggacca gcactgaaac ctgtactttg
     3181 cccagcttca gggagccagc ggtccggatc atttcgtccc actgaaggac tatttttcgg
     3241 ggatctgatt gccctctggc aatatcattc agcacgccat agtcggcatc atggtccatt
     3301 cgccagaaaa ccgaagcacc ggcatcagcc aggcgtggag aaaactggta tcccagcagc
     3361 cagaaaaggc caaagacaag ttcgctggca cctgctgtat cggtcataat ttcggttgga
     3421 ttcagcccgg tctcctgttc cagaagacct tccagcacaa agatagagtc cctcagcgtc
     3481 cccggtataa cgatgccatg aaagccggaa tactgatcgg acacaaagtt gtaccaggtg
     3541 atccctctgt tattaccaaa gtatttgcgg ttcggtccgg cattgattgt tctgactggc
     3601 gtaacaaagc gcattccatc tgcagatgcc acttctcctc caccccatat ctgtgccagt
     3661 ggcagcgttg cctgaaaatc aaccagtctg gcattagcgc tggtgatagt ttcagcccgc
     3721 agatagttcg cttttgtcca gttcagccgg tgtcgggtca gtgcaggaac atttgatctg
     3781 atcagtggtt ccagaccgat attgcaggct tcagccatca gcacggcgct gatgctgacg
     3841 ggcagatcat caactctggc actggcttca ctagcatgga aaaactcatc agcaaatccg
     3901 gtatgggcgt taatttcgag cagcaactcc gttaaatcca ccggagggag tagatcactg
     3961 atcattttgc tcagtcgttt cagactgtcc ggctcatcaa gactggcgag gggagaaatt
     4021 gtcaaccggg gcttcgggcc agaaacatcg agttcgacag cctcattttc gcaaagacgt
     4081 gcagcaacct gtctgtaacg actatcaagc tgatgaccca gagattttat tgcttcctgc
     4141 gggtctgtcg ggtgccccaa agaacgataa accttaatcc ggtttgcctg ccagtcagca
     4201 ccctgtagta atcttgcacg aggatctccc caccggttac tgccggtaac gtagacatcc
     4261 ctccgcctca gactatcctg cagtttactg agaaagcaga gcgtgtatcc cctgcgggtg
     4321 atatgttttt ccttgttaat caccagccgt ttccatgacc gactgataat ttccgttggt
     4381 gcgtcgtcaa aaaactgccg ccgtgagctg aactcccggc tgaggtagtc acaggcattc
     4441 agagtggtaa ccccggcagg tgcggatgaa aatttaacgg tattcagcag atggggcagg
     4501 aaacgacgaa cgcgcccgta ctgctccacc atttcttcat gaaaattatc gtctgagggc
     4561 cgggcaattt cacggacaag cgtgatgatt tcagccagct tttgccttgg gatgtagctg
     4621 aacacctcag cacgaatcga ttcgtccggt gtttcttctt tcagcaggta cgaacatgcg
     4681 ctggcgagcg ccaatgcaga tttatccaga tccttcagcg agcggagccg ttttttctgc
     4741 ccaatctttc tggcgtcacg gatgataacg gccagcatgg cgtccagaac gtccaatgca
     4801 tcatccagcg ccagcgtttc ccatgcaagg acaaaggcaa ccagaaccgc catccttttc
     4861 tgcggtgaca tcctggcaat attgaacacc gaagtcatac cagcataacg tgcgagattt
     4921 ttcaggcgca cagccgggag tgtactcagg ttttcagcat gcaggccaaa
//
LOCUS       DAZOYF010000551         4314 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.guided.245, whole genome shotgun sequence.
ACCESSION   DAZOYF010000551 DAZOYF010000000
VERSION     DAZOYF010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.guided.245"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            58..531
                     /gene="dfrA5"
                     /locus_tag="ACHUOF_004729"
     CDS             58..531
                     /gene="dfrA5"
                     /locus_tag="ACHUOF_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA5"
                     /protein_id="HFR0613537.1"
                     /translation="MKVSLMAAKAKNGVIGCGPHIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSAWTADNDNVIVFPSIEEAMYGLAELTDHVIVSGGGEI
                     YRETLPMASTLHISTIDIEPEGDVFFPNIPNTFEVVFEQHFSSNINYCYQIWQKG"
     gene            715..1062
                     /locus_tag="ACHUOF_004730"
     CDS             715..1062
                     /locus_tag="ACHUOF_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR0613538.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            1056..1895
                     /gene="sul1"
                     /locus_tag="ACHUOF_004731"
     CDS             1056..1895
                     /gene="sul1"
                     /locus_tag="ACHUOF_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR0613539.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            2023..2523
                     /locus_tag="ACHUOF_004732"
     CDS             2023..2523
                     /locus_tag="ACHUOF_004732"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0613540.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     gene            complement(2642..3394)
                     /gene="umuC"
                     /locus_tag="ACHUOF_004733"
                     /pseudo
     CDS             complement(2642..3394)
                     /gene="umuC"
                     /locus_tag="ACHUOF_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
     gene            complement(3394..3831)
                     /gene="umuD"
                     /locus_tag="ACHUOF_004734"
     CDS             complement(3394..3831)
                     /gene="umuD"
                     /locus_tag="ACHUOF_004734"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR0613541.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(3828..4076)
                     /locus_tag="ACHUOF_004735"
     CDS             complement(3828..4076)
                     /locus_tag="ACHUOF_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0613542.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
BASE COUNT          912 a         1194 c         1175 g         1033 t
ORIGIN      
        1 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aacccggaac caaaattgtg
       61 aaagtatcat taatggctgc aaaagcgaaa aacggagtga ttggttgcgg tccacacata
      121 ccctggtccg cgaaaggaga gcagctactc tttaaagcct tgacgtacaa ccagtggctt
      181 ttggtgggcc gcaagacgtt cgaatctatg ggagcactcc ctaataggaa atacgcggtc
      241 gttactcgct cagcctggac ggccgataat gacaacgtaa tagtattccc gtcgatcgaa
      301 gaggccatgt acgggctggc tgaactcacc gatcacgtta tagtgtctgg tggcggggag
      361 atttacagag aaacattgcc catggcctct acgctccata tatcgacgat tgatattgag
      421 ccggaaggag atgttttctt tccgaatatt cccaatacct tcgaagttgt ttttgagcaa
      481 cactttagct caaacattaa ctattgctat caaatttggc aaaagggtta acaaagctat
      541 gcaattgacg gtaaaaagct tcgttcgctt cgcttgctac gcttcttacc gcaattgata
      601 acggcgttag atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc
      661 ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgaaa
      721 ggctggcttt ttcttgttat cgcaatagtt ggcgaagtaa tcgcaacatc cgcattaaaa
      781 tctagcgagg gctttactaa gcttgcccct tccgccgttg tcataatcgg ttatggcatc
      841 gcattttatt ttctttctct ggttctgaaa tccatccctg tcggtgttgc ttatgcagtc
      901 tggtcgggac tcggcgtcgt cataattaca gccattgcct ggttgcttca tgggcaaaag
      961 cttgatgcgt ggggctttgt aggtatgggg ctcataattg ctgccttttt gctcgcccga
     1021 tccccatcgt ggaagtcgct gcggaggccg acgccatggt gacggtgttc ggcattctga
     1081 atctcaccga ggactccttc ttcgatgaga gccggcggct agaccccgcc ggcgctgtca
     1141 ccgcggcgat cgaaatgctg cgagtcggat cagacgtcgt ggatgtcgga ccggccgcca
     1201 gccatccgga cgcgaggcct gtatcgccgg ccgatgagat cagacgtatt gcgccgctct
     1261 tagacgccct gtccgatcag atgcaccgtg tttcaatcga cagcttccaa ccggaaaccc
     1321 agcgctatgc gctcaagcgc ggcgtgggct acctgaacga tatccaagga tttcctgacc
     1381 ctgcgctcta tcccgatatt gctgaggcgg actgcaggct ggtggttatg cactcagcgc
     1441 agcgggatgg catcgccacc cgcaccggtc accttcgacc cgaagacgcg ctcgacgaga
     1501 ttgtgcggtt cttcgaggcg cgggtttccg ccttgcgacg gagcggggtc gctgccgacc
     1561 ggctcatcct cgatccgggg atgggatttt tcttgagccc cgcaccggaa acatcgctgc
     1621 acgtgctgtc gaaccttcaa aagctgaagt cggcgttggg gcttccgcta ttggtctcgg
     1681 tgtcgcggaa atccttcttg ggcgccaccg ttggccttcc tgtaaaggat ctgggtccag
     1741 cgagccttgc ggcggaactt cacgcgatcg gcaatggcgc tgactacgtc cgcacccacg
     1801 cgcctggaga tctgcgaagc gcaatcacct tctcggaaac cctcgcgaaa tttcgcagtc
     1861 gcgacgccag agaccgaggg ttagatcatg cctagcattc accttccggc cgcccgctag
     1921 cggaccctgg tcaggttccg cgaaggtggg cgcagacatg ctgggctcgt caggatcaaa
     1981 ctgcactatg aggcggcggt tcataccgcg ccaggggagc gaatggacag cgaggagcct
     2041 ccgaacgttc gggtcgcctg ctcgggtgat atcgacgagg ttgtgcggct gatgcacgac
     2101 gctgcggcgt ggatgtccgc caagggaacg cccgcctggg acgtcgcgcg gatcgaccgg
     2161 acattcgcgg agaccttcgt cctgagatcc gagctcctag tcgcgagttg cagcgacggc
     2221 atcgtcggct gttgcacctt gtcggccgag gatcccgagt tctggcccga cgccctcaag
     2281 ggggaggccg catatctgca caagctcgcg gtgcgacgga cacatgcggg ccggggtgtc
     2341 agctccgcgc tgatcgaggc ttgccgccat gccgcgcgaa cgcaggggtg cgccaagctg
     2401 cggctcgact gccacccgaa cctgcgtggc ctatacgagc ggctcggatt cacccacgtc
     2461 gacactttca atcccggctg ggatccaacc ttcatcgcag aacgcctaga actcgaaatc
     2521 taacgtccgt tcgggcatcg aggtccatgt cggggtggga cgggcccgtg gcttcaagat
     2581 cacttgcagt ccgaccgcga tgtcttggtt gcgcgagagg ttgtcgatat cctccacttc
     2641 cactacagac aatctgctgt tttgccggcg gtgcttcttc cagagatatg caggactcgc
     2701 cgttgagttc gcgtaccgta cgctcaagaa tgacgctgaa gtttttccgg atgaatgccg
     2761 tgttagcctg cgccagctgc agtgctgtgt taatacccag cgcattcagc ttttccgtca
     2821 gtctgcgtcc tactccccag acctcaccaa ctggctgcaa ccccagtagc ttcaagatcc
     2881 gattacggtt ttctgccgtc agcgcgacca caccggaaaa ctgtggccat tgctttgttg
     2941 cccactgggc acttttagcc agcgtttttg taggcgcaat gcccaccccc atggtgagtc
     3001 ctgtccagct ctttacctgt tccctgagct gatgaccaaa aatctccgga gagatgcaat
     3061 gatttatccc ccgcaaatca atgaacattt catcaattga gtagggctca actgcgggag
     3121 aaagcgactc cagaacagcc ataacccgtt ggctcatgct gtggtacagc gcataattgc
     3181 tggaaaatac atgtattttc ttctccaggc gcatttgtct cacctgaaac cagggctgcc
     3241 ccattctgat gccaagggct tttgcctccg ggctgcgcgc gatcacacag ccatcgttat
     3301 tgctgagtac gatgaccggt tcgttgcgaa ggtccgggcg gaaaactttt tcacatgagg
     3361 cgtagaaact gttgatatca gccagtgcaa acatcagcgt aactcccgcg tcctgtgtat
     3421 cacgtgagtg acaacaccaa aaatacagat gttttccgga tacagtgtgc ggaactccgg
     3481 gctgtctgaa accggctcca gtgccgggcg tgggcgcaac agcagtcgtt tgacggtgaa
     3541 ctcaccgtcg atctcagcga taacgatgtc cccgtgttgt ggtttttcgg ccctgtccac
     3601 taccagcaga tcaccattct gcacgccagc ctggttcatc gattcaccgc tggcgcgcag
     3661 aaagaaggtg gctgcaggtc tgctgatgca atagctgttc agatccagtt cctgctcagc
     3721 ataatcagtg gcaggcgaag gaaaaccggc ctggcaacga tcggcaaaca acggtcgcac
     3781 gtaactgtca tcgcctgaag ggtcagccgg acggtgataa acggtactca tgctgcaggc
     3841 agccagctgt cgtcctgcca gatttcttcc agcagagaca ggatacgttc tttatccttg
     3901 tcgttgcggg ctcctgtgac agatacagac ggggcgctgc cctggtctat acgcagccag
     3961 atttccgggt agtgtggttt cagacgttgt tcgatttcat tctgtagcgc ctgaagtgtg
     4021 cctgatttga ggttttttgt gctctggcgg tcgaaaagaa tttcaatgcg gatcatgttc
     4081 tcatctccct tttctttatg ctgtatgttt atacagtatt ctaataactg aatccgcaca
     4141 cagtcaaatg tcattgttgt gactaaaaaa cgctcaattc tctgagtgtg tggctgtatg
     4201 cctgctgtgg gctgctgtgt ccagcttatc cacagcattt tgtgcacggt tctgtggaca
     4261 aaatacctgg ttacccaggc cgtgccggca cgttaaccgg gctgcatccg atgc
//
LOCUS       DAZOYF010000552         4295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.guided.246, whole genome shotgun sequence.
ACCESSION   DAZOYF010000552 DAZOYF010000000
VERSION     DAZOYF010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.guided.246"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            558..797
                     /locus_tag="ACHUOF_004736"
     CDS             558..797
                     /locus_tag="ACHUOF_004736"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR0613543.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHUOF_004737"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHUOF_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHUOF_004738"
     CDS             2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHUOF_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0613544.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="ACHUOF_004739"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="ACHUOF_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0613545.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4282
                     /gene="aadA1"
                     /locus_tag="ACHUOF_004740"
                     /pseudo
     CDS             3444..4282
                     /gene="aadA1"
                     /locus_tag="ACHUOF_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         1207 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     4141 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gttcactacg
     4201 tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac
     4261 gccgcttcgc ggcgcggctt aactcaagcg ttaga
//
LOCUS       DAZOYF010000553         4292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.guided.247, whole genome shotgun sequence.
ACCESSION   DAZOYF010000553 DAZOYF010000000
VERSION     DAZOYF010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.guided.247"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            558..797
                     /locus_tag="ACHUOF_004741"
     CDS             558..797
                     /locus_tag="ACHUOF_004741"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR0613546.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHUOF_004742"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHUOF_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHUOF_004743"
     CDS             2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHUOF_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0613547.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="ACHUOF_004744"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="ACHUOF_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0613548.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4279
                     /gene="aadA1"
                     /locus_tag="ACHUOF_004745"
                     /pseudo
     CDS             3444..4279
                     /gene="aadA1"
                     /locus_tag="ACHUOF_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         1204 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag gcttatcttg
     4141 gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
     4201 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
     4261 gcttcgcggc gcggcttaac tcaagcgtta ga
//
LOCUS       DAZOYF010000554         2829 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.guided.301, whole genome shotgun sequence.
ACCESSION   DAZOYF010000554 DAZOYF010000000
VERSION     DAZOYF010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.guided.301"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            84..641
                     /locus_tag="ACHUOF_004746"
     CDS             84..641
                     /locus_tag="ACHUOF_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0613549.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            824..1684
                     /locus_tag="ACHUOF_004747"
     CDS             824..1684
                     /locus_tag="ACHUOF_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR0613550.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1832..2257)
                     /locus_tag="ACHUOF_004748"
                     /pseudo
     CDS             complement(1832..2257)
                     /locus_tag="ACHUOF_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            complement(2395..>2829)
                     /locus_tag="ACHUOF_004749"
     CDS             complement(2395..>2829)
                     /locus_tag="ACHUOF_004749"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFR0613551.1"
                     /translation="QGLHGCQHALRGRPVLFPNFSLGEEYEHAPPATNRQISPYLPSG
                     RFRTGLPVEGLAIERGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLS
                     SVAWASDADYDVRLVQDCCYDPDRDAHEALLRSGFGGRVQVV"
BASE COUNT          725 a          704 c          751 g          649 t
ORIGIN      
        1 ctgttacatt aatatgtcta ttaaatcgta aatttgtaat aatagacatg agttgtccga
       61 tattcgattt aaggtacatt tttatgcgac tttttggtta cgctcgggtc tcaaccagtc
      121 agcagtctct tgatcttcag gtcagagcac tcaaagacgc aggtgtgaaa gcaaaccgta
      181 tatttaccga taaggcatcc ggcagttcaa cagaccggga agggctggat ttgctgagga
      241 tgaaggtgga ggaaggtgat gtcattctgg ttaagaagct cgaccgtctt ggccgcgaca
      301 ctgccgatat gatccaactg ataaaggaat ttgacgctca gggcgtggca gtccggttca
      361 ttgatgacgg gatcagtacc gacggtgata tggggcaaat ggtggtcacc atcctgtcgg
      421 ctgtggcaca ggctgaacgc cggaggatcc tagaacgcac gaatgagggc cgacaggaag
      481 caaagctgaa aggaatcaaa tttggccgca ggcgtaccgt ggacaggaac gtcgtgctga
      541 cgcttcatca gaagggcact ggtgcaacgg aaattgctca tcagctcagt attgcccgct
      601 ccacggttta taaaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
      661 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      721 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      781 ataaccctgg taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      841 tcgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      901 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      961 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     1021 gatgagcact tttaaagttc tgctatgtgg tgcggtatta tcccgtgttg acgccgggca
     1081 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     1141 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1201 catgagtgat aacactgctg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1261 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1321 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac
     1381 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1441 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1501 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1561 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1621 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1681 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1741 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1801 tgagttttcg ttccactgag cgtcagaccc catctagcgc tgcgtttggt gtcaacacca
     1861 acagcgccac gcccgcagtt ccgcaaatag cccccaggac cgccatcaat cgtatcgggc
     1921 tacctagcag agcggcagag atgaacacga ccatcagcgg ctgcacagcg cctaccgtcg
     1981 ccgcgacccc gcccggcagg cggtagaccg aaataaacaa caagctccag aatagcgaaa
     2041 tattaagtgc gccgaggatg aagatgcgca tccaccagat tcccgttgga atctgtcgga
     2101 cgatcatcac gagcaataaa cccgccggca acgcccgcag catcgcgacc gtcatcggtg
     2161 agaagttcgg caggtattgg gtggtgacaa tgtaggtgct gccccaaatg gcaggtgcta
     2221 tcgctgtgaa caataaatcg ggcgtgcgta atgacatgcg attacaagac ctccgctacg
     2281 gcgatgggtt tttgagagtt gcaggcgccg cgatgaccgc accctgactt ggactggccc
     2341 tgcctcgcac caatccacct gcggctatgg ccgcgatcat gcgaggcccg aaaattacac
     2401 gacctgtaca cgtccgccga acccggaacg caacagagct tcgtgggcat cccgatccgg
     2461 gtcgtagcag cagtcctgga ccaaacgcac gtcgtagtcc gcatcactag cccaggcgac
     2521 gcttgaaaga acaacgccgg tggtgcttat cccggccatg acaagcgtgc ttacgccgcg
     2581 cgtccgaagg tcggcgtcga gcgccgtgcc ataaaagacg ctggctcgcg gacatgcata
     2641 gaaaaggtcg ccccgttcga tcgcaagccc ttcgacgggc agaccggtgc gaaatcgtcc
     2701 gctcgggaga tacggtgaga tttggcgatt cgtcgccggc ggcgcatgtt catactcttc
     2761 gccgagcgaa aagttgggga acagcacggg ccggccgcgc aaggcgtgct ggcagccgtg
     2821 cagaccctg
//
LOCUS       DAZOYF010000555         2584 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.guided.309, whole genome shotgun sequence.
ACCESSION   DAZOYF010000555 DAZOYF010000000
VERSION     DAZOYF010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.guided.309"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            39..428
                     /locus_tag="ACHUOF_004750"
                     /pseudo
     CDS             39..428
                     /locus_tag="ACHUOF_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(406..1029)
                     /gene="tetR(B)"
                     /locus_tag="ACHUOF_004751"
     CDS             complement(406..1029)
                     /gene="tetR(B)"
                     /locus_tag="ACHUOF_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR0613552.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1111..2316
                     /gene="tet(B)"
                     /locus_tag="ACHUOF_004752"
     CDS             1111..2316
                     /gene="tet(B)"
                     /locus_tag="ACHUOF_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR0613553.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2429..>2584)
                     /locus_tag="ACHUOF_004753"
     CDS             complement(2429..>2584)
                     /locus_tag="ACHUOF_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFR0613554.1"
                     /translation="LKAYDDDVLKNLLNGWFMHIAIHAKNLKELADKKGQFIAIYRGF
                     LLSLKDK"
BASE COUNT          671 a          502 c          549 g          862 t
ORIGIN      
        1 tgaacataaa acactatcaa taagttggag tcattaccaa ccatggcgta catgccaaag
       61 tttccacgcc agtgcattta cgaaaagcac gtacttgcta tgatcattta gctggcgaag
      121 ttgccgttaa gatctatgat tccctttgtc aacagcaatg gatcactgaa aatggttcaa
      181 tgatcacatt aagtggtatt caatattttc atgaaatggg aattgacgtt ccttccaaac
      241 attcacgtaa aatctgttgt gcgtgtttag attggagtga acgccgtttc catttaggtg
      301 ggtacgttgg agccgcatta ttttcgcttt atgaatctaa agggtggtta actcgacatc
      361 ttggttaccg tgaagttacc atcacggaaa aaggttatgc tgcttttaag acccactttc
      421 acatttaagt tgtttttcta atccgcatat gatcaattca aggccgaata agaaggctgg
      481 ctctgcacct tggtgatcaa ataattcgat agcttgtcgt aataatggcg gcatactatc
      541 agtagtaggt gtttcccttt cttctttagc gacttgatgc tcttgatctt ccaatacgca
      601 acctaaagta aaatgcccca cagcgctgag tgcatataat gcattctcta gtgaaaaacc
      661 ttgttggcat aaaaaggcta attgattttc gagagtttca tactgttttt ctgtaggccg
      721 tgtacctaaa tgtacttttg ctccatcgcg atgacttagt aaagcacatc taaaactttt
      781 agcgttatta cgtaaaaaat cttgccagct ttccccttct aaagggcaaa agtgagtatg
      841 gtgcctatct aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat tttttacatg
      901 ccaatacaat gtaggctgct ctacacctag cttctgggcg agtttacggg ttgttaaacc
      961 ttcgattccg acctcattaa gcagctctaa tgcgctgtta atcactttac ttttatctaa
     1021 tctagacatc attaattcct aatttttgtt gacactctat cattgataga gttattttac
     1081 cactccctat cagtgataga gaaaagtgaa atgaatagtt cgacaaagat cgcattggta
     1141 attacgttac tcgatgccat ggggattggc cttatcatgc cagtcttgcc aacgttatta
     1201 cgtgaattta ttgcttcgga agatatcgct aaccactttg gcgtattgct tgcactttat
     1261 gcgttaatgc aggttatctt tgctccttgg cttggaaaaa tgtctgaccg atttggtcgg
     1321 cgcccagtgc tgttgttgtc attaataggc gcatcgctgg attacttatt gctggctttt
     1381 tcaagtgcgc tttggatgct gtatttaggc cgtttgcttt cagggatcac aggagctact
     1441 ggggctgtcg cggcatcggt cattgccgat accacctcag cttctcaacg cgtgaagtgg
     1501 ttcggttggt taggggcaag ttttgggctt ggtttaatag cggggcctat tattggtggt
     1561 tttgcaggag agatttcacc gcatagtccc ttttttatcg ctgcgttgct aaatattgtc
     1621 gctttccttg tggttatgtt ttggttccgt gaaaccaaaa atacacgtga taatacagat
     1681 accgaagtag gggttgagac gcaatcgaat tcggtataca tcactttatt taaaacgatg
     1741 cccattttgt tgattattta tttttcagcg caattgatag gccaaattcc cgcaacggtg
     1801 tgggtgctat ttaccgaaaa tcgttttgga tggaatagca tgatggttgg cttttcatta
     1861 gcgggtcttg gtcttttaca ctcagtattc caagcctttg tggcaggaag aatagccact
     1921 aaatggggcg aaaaaacggc agtactgctc ggatttattg cagatagtag tgcatttgcc
     1981 tttttagcgt ttatatctga aggttggtta gttttccctg ttttaatttt attggctggt
     2041 ggtgggatcg ctttacctgc attacaggga gtgatgtcta tccaaacaaa gagtcatcag
     2101 caaggtgctt tacagggatt attggtgagc cttaccaatg caaccggtgt tattggccca
     2161 ttactgtttg ctgttattta taatcattca ctaccaattt gggatggctg gatttggatt
     2221 attggtttag cgttttactg tattattatc ctgctatcga tgaccttcat gttaacccct
     2281 caagctcagg ggagtaaaca ggagacaagt gcttagttat ttcgtcacca aatgatgtta
     2341 ttccgcgaaa tataatgacc ctcttgataa cccaagaggg cattttttac gataaagaag
     2401 atttagcttc aaataaaacc tatctatttt atttatcttt caagctcaat aaaaagccgc
     2461 ggtaaatagc aataaattgg ccttttttat cggcaagctc ttttaggttt ttcgcatgta
     2521 ttgcgatatg cataaaccag ccattgagta agtttttaag cacatcatca tcataagctt
     2581 taag
//
LOCUS       DAZOYF010000556         2117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.guided.342, whole genome shotgun sequence.
ACCESSION   DAZOYF010000556 DAZOYF010000000
VERSION     DAZOYF010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.guided.342"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            1..1127
                     /locus_tag="ACHUOF_004754"
                     /pseudo
     CDS             1..1127
                     /locus_tag="ACHUOF_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            1216..1749
                     /gene="blc"
                     /locus_tag="ACHUOF_004755"
     CDS             1216..1749
                     /gene="blc"
                     /locus_tag="ACHUOF_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0613555.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1746..2063)
                     /gene="sugE"
                     /locus_tag="ACHUOF_004756"
     CDS             complement(1746..2063)
                     /gene="sugE"
                     /locus_tag="ACHUOF_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0613556.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
BASE COUNT          540 a          561 c          532 g          484 t
ORIGIN      
        1 aaaacgacgc tctgcgcctt attaattacc gcctcttact ccacatttgc tgcccctcaa
       61 caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca aaagatcccc
      121 ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacctg gggctatgcg
      181 gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg ttcggtcagc
      241 aaaacattta cgggcgtgct tggtggcgac gctattgctc gaggggaaat caagttaagc
      301 gatcccgcaa caaaatactg gcctgaactt accgctaaac agtggaatgg gatcacacta
      361 ttacatcttg cgacctacac cgctggcggc ctgccattgc aggtgccgga tgaagtgaaa
      421 tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc tccaggaaca
      481 caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt gaagccgtct
      541 ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc agccactcaa actcaaccat
      601 acgtggatta atgtacctcc cccagaagaa aagaattacg cctggggata tcgcgaaggt
      661 aaggcagtgc atgtttcgcc aggggcgtta gatgctgaag cttatggtgt gaagtcgacc
      721 attgaagata tggcccgctg ggtgcaaagc aatttacccc ccttgatatc aatgagaaaa
      781 cgcttcaaca agggatacaa ctggcacaat ctcgctactg gcaaaccggc gatatgtatc
      841 agggcctggg ctgggaaatg ctggactggc cggtcaatcc tgacagcatc attaacggca
      901 gtgacaataa aattgcactg gcagcacgcc cggtaaaagc gattaagccc ccaactcctg
      961 cagtacgcgc atcatgggta cataaaacgg gggcgaccgg cggatttggt agctatgtcg
     1021 cgtttattcc agaaaaagag ctgggtatcg tgatgctggc taacaaaaac tatcccaatc
     1081 cagcgagagt cgccgccgcc tgtcagattc tcaacgctct acagtaaaat ttcatcgggt
     1141 ccgaattttc ggaccttttc tgcgcttttc cttgctgtca tctacactta gaaaaaaacc
     1201 agtaaggaaa catttatgcg cctgctccct ctcgttgccg cagcgacagc tgcatttctg
     1261 gtcgttgcct gtagttttcc tacgccgccg cgtggcgtga ccgtagtgaa taatttcgac
     1321 gccaaacgtt atctcggtac ctggtatgag attgcccgtt ttgatcaccg ttttgaacgt
     1381 ggactggaaa aagtcaccgc aacatacagc ctgcgtgatg acggcggcct gaatgtcatt
     1441 aataaaggct ataaccctga cagaggaatg tggcagcaga gtgaagggaa agcgtacttt
     1501 accggcgcac caactcgcgc tgcgctgaaa gtgtcattct ttggtccttt ctatggcggt
     1561 tataacgtta ttgcactcga tcgggaatac cgccatgcgc tggtttgcgg cccggaccgc
     1621 gactacctgt ggatactctc ccgcacgcca accatttctg acgaagtgaa acaggagatg
     1681 ctggcagtcg cgacccggga agggtttgat gtcagtaagt ttatttgggt acagcagccc
     1741 ggtagttagt gagtgctgag tttcagacca ataatcccca atacgattaa cgccagactc
     1801 gccagacgca tcgggttagc ggactcaccg agcagcacaa tgccggtgat ggccgcgccg
     1861 actgcgccaa tacccgtcca cacggcataa gccgtcccta ccggtaacga tttcatcgcc
     1921 caggcaagta gcgccatact gactatcatc gccgtcacgg taataacact cggcgtcaaa
     1981 cggctaaagc cgtgggtata tttcaggcca acggcccata ccacttccag cagaccagca
     2041 ataactaaga taatccagga catatcaggc tccggaaatt tggggccgtc cccggtgaaa
     2101 agaggcgttt gcaggtc
//
LOCUS       DAZOYF010000557         1811 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.guided.370, whole genome shotgun sequence.
ACCESSION   DAZOYF010000557 DAZOYF010000000
VERSION     DAZOYF010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.guided.370"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            263..922
                     /locus_tag="ACHUOF_004757"
     CDS             263..922
                     /locus_tag="ACHUOF_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR0613557.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="ACHUOF_004758"
     CDS             complement(1123..1500)
                     /locus_tag="ACHUOF_004758"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0613558.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..>1811)
                     /locus_tag="ACHUOF_004759"
     CDS             complement(1567..>1811)
                     /locus_tag="ACHUOF_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFR0613559.1"
                     /translation="RYRASGLNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYL
                     SPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          467 a          431 c          445 g          468 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc t
//
LOCUS       DAZOYF010000558         1398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.guided.397, whole genome shotgun sequence.
ACCESSION   DAZOYF010000558 DAZOYF010000000
VERSION     DAZOYF010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.guided.397"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..434
                     /locus_tag="ACHUOF_004760"
     CDS             <1..434
                     /locus_tag="ACHUOF_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR0613560.1"
                     /translation="KIKNYLKDFDYGNQDFSGDKERNNGLTEAWLESSLKISPEEQIQ
                     FLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFI
                     ISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            547..1385
                     /gene="aadA1"
                     /locus_tag="ACHUOF_004761"
                     /pseudo
     CDS             547..1385
                     /gene="aadA1"
                     /locus_tag="ACHUOF_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          421 a          320 c          333 g          324 t
ORIGIN      
        1 ataaaatcaa gaattatctc aaagattttg attatggaaa tcaagacttc tctggagata
       61 aagaaagaaa caacggatta acagaagcat ggctcgaaag tagcttaaaa atttcaccag
      121 aagaacaaat tcaattcctg cgtaaaatta ttaatcacaa tctcccagtt aaaaactcag
      181 ccatagaaaa caccatagag aacatgtatc tacaagatct ggataatagt acaaaactgt
      241 atgggaaaac tggtgcagga ttcacagcaa atagaacctt acaaaacgga tggtttgaag
      301 ggtttattat aagcaaatca ggacataaat atgtttttgt gtccgcactt acaggaaact
      361 tggggtcgaa tttaacatca agcataaaag ccaagaaaaa tgcgatcacc attctaaaca
      421 cactaaattt ataaaaaatc taatggcaaa atcgcccaac ccttcaatca agtcgggacg
      481 gccaaaagca agcttttggc tcccctcgct ggcgctcggc gccccttatt tcaaacgtta
      541 aacatcatga gggaagtggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
      601 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
      661 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
      721 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
      781 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
      841 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
      901 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
      961 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     1021 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     1081 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     1141 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     1201 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
     1261 ttggcctcgc gcgcagatca gttggaagaa tttgttcact acgtgaaagg cgagatcacc
     1321 aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg
     1381 cttaactcaa gcgttaga
//
LOCUS       DAZOYF010000559         1395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.guided.398, whole genome shotgun sequence.
ACCESSION   DAZOYF010000559 DAZOYF010000000
VERSION     DAZOYF010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.guided.398"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            <1..434
                     /locus_tag="ACHUOF_004762"
     CDS             <1..434
                     /locus_tag="ACHUOF_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR0613561.1"
                     /translation="KIKNYLKDFDYGNQDFSGDKERNNGLTEAWLESSLKISPEEQIQ
                     FLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFI
                     ISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            547..1382
                     /gene="aadA1"
                     /locus_tag="ACHUOF_004763"
                     /pseudo
     CDS             547..1382
                     /gene="aadA1"
                     /locus_tag="ACHUOF_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          418 a          320 c          333 g          324 t
ORIGIN      
        1 ataaaatcaa gaattatctc aaagattttg attatggaaa tcaagacttc tctggagata
       61 aagaaagaaa caacggatta acagaagcat ggctcgaaag tagcttaaaa atttcaccag
      121 aagaacaaat tcaattcctg cgtaaaatta ttaatcacaa tctcccagtt aaaaactcag
      181 ccatagaaaa caccatagag aacatgtatc tacaagatct ggataatagt acaaaactgt
      241 atgggaaaac tggtgcagga ttcacagcaa atagaacctt acaaaacgga tggtttgaag
      301 ggtttattat aagcaaatca ggacataaat atgtttttgt gtccgcactt acaggaaact
      361 tggggtcgaa tttaacatca agcataaaag ccaagaaaaa tgcgatcacc attctaaaca
      421 cactaaattt ataaaaaatc taatggcaaa atcgcccaac ccttcaatca agtcgggacg
      481 gccaaaagca agcttttggc tcccctcgct ggcgctcggc gccccttatt tcaaacgtta
      541 aacatcatga gggaagtggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
      601 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
      661 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
      721 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
      781 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
      841 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
      901 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
      961 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     1021 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     1081 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     1141 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     1201 cagtatcagc ccgtcatact tgaagctagg caggcttatc ttggacaaga agatcgcttg
     1261 gcctcgcgcg cagatcagtt ggaagaattt gttcactacg tgaaaggcga gatcaccaag
     1321 gtagtcggca aataatgtct aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt
     1381 aactcaagcg ttaga
//
LOCUS       DAZOYF010000560         1102 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2107913
            SAMN43277615-rid23486583.guided.423, whole genome shotgun sequence.
ACCESSION   DAZOYF010000560 DAZOYF010000000
VERSION     DAZOYF010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277615
            Sequence Read Archive: SRR30464508
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396859.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396859.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:05:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,764
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,196
            CDSs (with protein)               :: 4,196
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 227 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 166 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277615-rid23486583.guided.423"
                     /strain="M2107913"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-11-04"
                     /collected_by="FSS"
     gene            109..939
                     /locus_tag="ACHUOF_004764"
     CDS             109..939
                     /locus_tag="ACHUOF_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HFR0613562.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          406 a          220 c          196 g          280 t
ORIGIN      
        1 acgatgttac gcagcagggc agtcgcccta aaacaaagtt gggcgaaccc ggagcctcat
       61 taattgttag ccgttaaaat taagcccttt accaaaccaa tacttattat gaaaaacaca
      121 atacatatca acttcgctat ttttttaata attgcaaata ttatctacag cagcgccagt
      181 gcatcaacag atatctctac tgttgcatct ccattatttg aaggaactga aggttgtttt
      241 ttactttacg atgcatccac aaacgctgaa attgctcaat tcaataaagc aaagtgtgca
      301 acgcaaatgg caccagattc aactttcaag atcgcattat cacttatggc atttgatgcg
      361 gaaataatag atcagaaaac catattcaaa tgggataaaa cccccaaagg aatggagatc
      421 tggaacagca atcatacacc aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa
      481 gaaataaccc aaaaaattgg attaaataaa atcaagaatt atctcaaaga ttttgattat
      541 ggaaatcaag acttctctgg agataaagaa agaaacaacg gattaacaga agcatggctc
      601 gaaagtagct taaaaatttc accagaagaa caaattcaat tcctgcgtaa aattattaat
      661 cacaatctcc cagttaaaaa ctcagccata gaaaacacca tagagaacat gtatctacaa
      721 gatctggata atagtacaaa actgtatggg aaaactggtg caggattcac agcaaataga
      781 accttacaaa acggatggtt tgaagggttt attataagca aatcaggaca taaatatgtt
      841 tttgtgtccg cacttacagg aaacttgggg tcgaatttaa catcaagcat aaaagccaag
      901 aaaaatgcga tcaccattct aaacacacta aatttataaa aaatctaatg gcaaaatcgc
      961 ccaacccttc aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc
     1021 tcggcgcccc ttatttcaaa cgttaaacat catgagggaa gtggtgatcg ccgaagtatc
     1081 gactcaacta tcagaggtag tt
//
